The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000.
We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.
Buell
C Robin
CR
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. rbuell@tigr.org
Joardar
Vinita
V
Lindeberg
Magdalen
M
Selengut
Jeremy
J
Paulsen
Ian T
IT
Gwinn
Michelle L
ML
Dodson
Robert J
RJ
Deboy
Robert T
RT
Durkin
A Scott
AS
Kolonay
James F
JF
Madupu
Ramana
R
Daugherty
Sean
S
Brinkac
Lauren
L
Beanan
Maureen J
MJ
Haft
Daniel H
DH
Nelson
William C
WC
Davidsen
Tanja
T
Zafar
Nikhat
N
Zhou
Liwei
L
Liu
Jia
J
Yuan
Qiaoping
Q
Khouri
Hoda
H
Fedorova
Nadia
N
Tran
Bao
B
Russell
Daniel
D
Berry
Kristi
K
Utterback
Teresa
T
Van Aken
Susan E
SE
Feldblyum
Tamara V
TV
D'Ascenzo
Mark
M
Deng
Wen-Ling
WL
Ramos
Adela R
AR
Alfano
James R
JR
Cartinhour
Samuel
S
Chatterjee
Arun K
AK
Delaney
Terrence P
TP
Lazarowitz
Sondra G
SG
Martin
Gregory B
GB
Schneider
David J
DJ
Tang
Xiaoyan
X
Bender
Carol L
CL
White
Owen
O
Fraser
Claire M
CM
Collmer
Alan
A
D017360:Arabidopsis / Q000382:microbiology*; D001483:Base Sequence; D001692:Biological Transport; D016680:Genome, Bacterial; D018551:Solanum lycopersicum / Q000382:microbiology*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000096:biosynthesis; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D017382:Reactive Oxygen Species; D017262:Siderophores / Q000096:biosynthesis; D014774:Virulence
D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D017262:Siderophores