title: [Use of polymerase chain reaction for determining T-cell clonality].
abstract: OBJECTIVE
  To distinguish T-cell lymphomas and reactive T-cell proliferation it is important to confirm the ability of T-cells to be cloned. Conventional histological and immunophenotypic methods fail to determine the ability of T-cells to be cloned. An experience in the use of detection of T-cell receptor gene gamma-chain (TCRy) rearrangement for determining T-cellular clonality is described.
  
  
  METHODS
  Polymerase chain reaction (PCR) and single strand conformational polymorphism (SSCP) were used to determine T-cell clonality. Twenty healthy donors, 28 patients with T-lymphomas, and 26 patients with various non-T-cell lymphoproliferative disorders or reactive processes were studied.
  
  
  RESULTS
  T-cell monoclonality was detected in 23/28 (82%) T-cell lymphoma cases, whereas in all the samples from normal subjects a polyclonal pattern of rearrangements TCRy was found. The sensitivity of the method was estimated as 2.5%, 7%, and 10% was demonstrated for bone marrow, spleen, and peripheral blood, respectively.
  
  
  CONCLUSIONS
  PCR-SSCP for TCRy was found to be a useful supplement to routine histological and immunophenotypic methods in the diagnosis of T-cell lymphomas.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Sidorova
authors.forename: E A
authors.initials: EA
authors.lastname: Nikitin
authors.forename: M
authors.initials: M
authors.lastname: Peklo
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.forename: R S
authors.initials: RS
authors.lastname: Samoĭlova
authors.forename: S K
authors.initials: SK
authors.lastname: Kravchenko
authors.forename: A L
authors.initials: AL
authors.lastname: Melikian
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Vinogradova
authors.forename: A V
authors.initials: AV
authors.lastname: Pivnik
authors.forename: A B
authors.initials: AB
authors.lastname: Sudarikov
mesh_terms: D002999:Clone Cells / Q000473:pathology; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D016393:Lymphoma, B-Cell / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000473:pathology*
chemical_list: D017931:DNA Primers; D004247:DNA