Neuronal untranslated BC1 RNA: targeted gene elimination in mice.
Despite the potentially important roles of untranslated RNAs in cellular form or function, genes encoding such RNAs have until now received surprisingly little attention. One such gene encodes BC1 RNA, a small non-mRNA that is delivered to dendritic microdomains in neurons. We have now eliminated the BC1 RNA gene in mice. Three independent founder lines were established from separate embryonic stem cells. The mutant mice appeared to be healthy and showed no anatomical or neurological abnormalities. The gross brain morphology was unaltered in such mice, as were the subcellular distributions of two prototypical dendritic mRNAs (encoding MAP2 and CaMKIIalpha). Due to the relatively recent evolutionary origin of the gene, we expected molecular and behavioral consequences to be subtle. Behavioral analyses, to be reported separately, indicate that the lack of BC1 RNA appears to reduce exploratory activity.
Skryabin
Boris V
BV
Institute of Experimental Pathology (ZMBE), University of Münster, Von-Esmarch Strasse 56, D-48149 Münster, Germany. RNA.world@uni-muenster.de
Sukonina
Valentina
V
Jordan
Ursula
U
Lewejohann
Lars
L
Sachser
Norbert
N
Muslimov
Ilham
I
Tiedge
Henri
H
Brosius
Jürgen
J
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D003712:Dendrites / Q000502:physiology; D005260:Female; D015870:Gene Expression; D018390:Gene Targeting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics*; D012016:Reference Values
C117734:BC1 RNA; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D020733:RNA, Small Cytoplasmic; D022661:RNA, Untranslated; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases