title: [Expression of heme oxygenase-1 in rat prostate and effects of androgen and estrogen on it].
abstract: OBJECTIVE
  To investigate the influences of androgen and estrogen on expression of HO-1 gene in rat ventral prostate.
  
  
  METHODS
  To create the model of castrated male rats, the testes were removed via scrotal incision. HO-1 mRNA level was tested by RT-PCR. The use of immunohistochemical staining procedures with image analytic technical system allowed us to assess the effects of exogenous androgen and estrogen on expression and location of HO-1 protein in rat ventral prostate.
  
  
  RESULTS
  The levels of HO-1 mRNA transcription and HO-1 protein expression in the castrated group were markedly decreased, compared with those in normal control group (P < 0.01); But both these levels in the groups of castration plus exogenous androgen and castration plus exogenous estrogen were much higher than those in the control group (P < 0.01). Furthermore, estrogen could increase the level of HO-1 protein expression in prostatic stroma.
  
  
  CONCLUSIONS
  Carbon monoxide-heme oxygenase system might be involved in the pathologic processes of prostatic abnormal proliferation.
authors.affiliation: Department of Physiology, Chengdu Military Medical College of Third Military University, Chengdu 610083, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tian
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004967:Estrogens / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D000728:Androgens; D004967:Estrogens; D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing)