[The induction of apoptosis and reactive oxygen species in human skin fibroblast by ultraviolet A].
OBJECTIVE
To analyse the induction of apoptosis and reactive oxygen species (ROS) in human skin fibroblast by ultraviolet A (UVA).


METHODS
Human skin fibroblasts were irradiated with UVA, apoptosis was determined with flowcytometry and fluorescent microscopy, the intracellular ROS production was measured with a spectrofluorometer, and the free radicals were determined with electron spin resonance.


RESULTS
A significant increase of apoptosis was observed in human skin fibroblast after exposure to UVA with a dosage at 110-120 kJ.m-2, mannitol could counteract apoptosis; a higher level of intracellular ROS and the hydroxyl free radical was detected in the irradiated samples.


CONCLUSIONS
A lower dose of irradiation of UVA(0.6 mW.cm-2) can induce apoptosis in human skin fibroblast, and ROS might play a role in the induction of apoptosis.
Wang
Hongwei
H
Department of Toxicology, Peking University School of Public Health, Beijing 100083, China.
Shang
Lanqin
L
Hao
Weidong
W
D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000528:radiation effects; D005609:Free Radicals; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D017304:Annexin A5; D005609:Free Radicals; D017382:Reactive Oxygen Species