title: Carotenoid cleavage products modify respiratory burst and induce apoptosis of human neutrophils.
abstract: Carotenoid supplementation in the treatment of diseases associated with oxidative stress has been recently questioned because of the cell damage and the increased risk of lung cancer in male smokers. Because of the complex role of neutrophils in lung diseases, we investigated whether carotenoid derivatives could affect respiratory burst and apoptosis of human neutrophils purified from peripheral blood. Stimulation of superoxide production was induced by nanomolar and micromolar concentrations of carotenoid cleavage products with aliphatic chains of different length, but not by carotenoids lacking the carbonyl moiety. The stimulatory effect of carotenoid cleavage products was observed in cells activated by phorbol myristate acetate (PMA), while a slight inhibition of superoxide production was noticed with cells activated by the chemotactic tripeptide N-formyl-Met-Leu-Phe (f-MLP). At higher concentrations, carotenoid cleavage products inhibited superoxide production in the presence of both PMA and f-MLP. In the presence of 20 microM carotenoid cleavage products, inhibition of superoxide production was accompanied by DNA fragmentation and increased level of intracellular caspase-3 activity.
authors.affiliation: Herzog Julius Hospital for Rheumatology and Orthopaedics Bad Harzburg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Siems
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Capuozzo
authors.forename: Carlo
authors.initials: C
authors.lastname: Crifò
authors.forename: Olaf
authors.initials: O
authors.lastname: Sommerburg
authors.forename: Claus-Dieter
authors.initials: CD
authors.lastname: Langhans
authors.forename: Lance
authors.initials: L
authors.lastname: Schlipalius
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wiswedel
authors.forename: Klaus
authors.initials: K
authors.lastname: Kraemer
authors.forename: Costantino
authors.initials: C
authors.lastname: Salerno
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002338:Carotenoids / Q000378:metabolism*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology*; D016897:Respiratory Burst / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013481:Superoxides; D002338:Carotenoids; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases