title: Trafficking of Th1 cells to lung: a role for selectins and a P-selectin glycoprotein-1-independent ligand.
abstract: Trafficking of lymphocytes to lung is a critical component of pulmonary immune defense and surveillance. Selectins, expressed on vascular endothelium, regulate T lymphocyte emigration into tissues, such as skin, but the role of the selectins in trafficking of T cells to lung has not been well characterized. Here, we used a model of lung inflammation induced by adoptive transfer of alloreactive Th1 cells to analyze the role of P- and E-selectin in Th1 cell trafficking to lung in vivo. We found that both P- and E-selectin play an important role in Th1 lymphocyte migration to lung. We confirmed that the Th1 cells express P-selectin glycoprotein ligand-1, which was functional in binding to P- and E-selectin in vitro. However, our studies reveal that a ligand distinct from P-selectin glycoprotein-1 also binds these selectins in vitro and appears to play a physiologic role in in vivo emigration of Th1 lymphocytes into the lung.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington, Seattle, WA 98109, USA. jclark@fhcrc.org
authors.forename: Joan G
authors.initials: JG
authors.lastname: Clark
authors.forename: Janis B
authors.initials: JB
authors.lastname: Mandac-Dy
authors.forename: Anne E
authors.initials: AE
authors.lastname: Dixon
authors.forename: David K
authors.initials: DK
authors.lastname: Madtes
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Burkhart
authors.forename: John M
authors.initials: JM
authors.lastname: Harlan
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Bullard
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009439:Neuraminidase / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019087:Selectins / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D011993:Recombinant Fusion Proteins; D019087:Selectins; D009439:Neuraminidase