[Studies on the interaction mode between polymyxin B sulfate and DNA with EB as a fluorescence probe].
The interaction between polymyxin B sulfate and ct-DNA was studied with ethidium bromide (EB) probe by UV spectroscopy, fluorescence spectroscopy, fluorescent polarization etc. The phenomena of hyperchromism effect and fluorescence quenching proved that polymyxin B sulfate in buffer solution at pH 7.4 can intercalate into the base pairs of ct-DNA. This interaction can cause conformational change of ct-DNA double helix. This interaction can be inhibited in the presence of high concentration of ions. At the same time, PB can combine with the phosphate group of ct-DNA by non-characteristic static force. In this paper, the interaction of PB and ct-DNA was made further discussion.
Jiang
Chong-qiu
CQ
Shandong Normal University, Jinan 250014, China.
He
Ji-xiang
JX
Wang
Jin-shan
JS
D004247:DNA / Q000737:chemistry*; D004347:Drug Interactions; D004996:Ethidium; D005456:Fluorescent Dyes; D007364:Intercalating Agents / Q000737:chemistry*; D011112:Polymyxin B / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D004247:DNA; D004996:Ethidium; D011112:Polymyxin B