[Role of different isoforms of nitric oxide synthase in development of tumor mutants in Drosophila melanogaster].
We studied the role of nitric oxide synthase during tumor growth in oncovirus-induced tumor mutants of Drosophila melanogaster. The lines with different capacity for malignancy differed reliably in the level of enzymatic activity. It was shown using specific inhibitors of neuronal and inducible isoforms that the neuronal isoform was not involved in tumor formation, while the inducible one appears to play an important role in tumor growth inhibition. This isoform was identified with the help of immunoblotting and monoclonal antibodies against inducible nitric oxide synthase.
Dzhansugurova
L B
LB
Al-Farabi Kazakh National University, Institute of General Genetics and Cytology, Ministry of Education and Science, prosp. Al-Farabi 75, Almaty, 480060 Kazakhstan.
Bekmanov
B O
BO
Bersimbaev
R I
RI
D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007814:Larva; D009154:Mutation; D009369:Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D012190:Retroviridae / Q000235:genetics
D004791:Enzyme Inhibitors; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II