title: Role for Salmonella enterica enterobacterial common antigen in bile resistance and virulence.
abstract: Passage through the digestive tract exposes Salmonella enterica to high concentrations of bile salts, powerful detergents that disrupt biological membranes. Mutations in the wecD or wecA gene, both of which are involved in the synthesis of enterobacterial common antigen (ECA), render S. enterica serovar Typhimurium sensitive to the bile salt deoxycholate. Competitive infectivity analysis of wecD and wecA mutants in the mouse model indicates that ECA is an important virulence factor for oral infection. In contrast, lack of ECA causes only a slight decrease in Salmonella virulence during intraperitoneal infection. A tentative interpretation is that ECA may contribute to Salmonella virulence by protecting the pathogen from bile salts.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville 41080, Spain. framos@us.es
authors.forename: Francisco
authors.initials: F
authors.lastname: Ramos-Morales
authors.forename: Ana I
authors.initials: AI
authors.lastname: Prieto
authors.forename: Carmen R
authors.initials: CR
authors.lastname: Beuzón
authors.forename: David W
authors.initials: DW
authors.lastname: Holden
authors.forename: Josep
authors.initials: J
authors.lastname: Casadesús
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001647:Bile Acids and Salts / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000503:physiopathology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001647:Bile Acids and Salts; C017286:enterobacterial common antigen; D003840:Deoxycholic Acid