Effect of the concentration of ionogenic groups in the sorbent on the separation of protein mixtures.
Investigations of sequential and simultaneous sorption of protein pairs on ion-exchangers with various contents of ionogenic groups were carried out. The transition from synergism to competition was shown to take place with a decrease in the content of the ionogenic groups. Of significant importance also is the uniformity of ionogenic group distribution in the sorbent volume. An examination of the dynamics of sorption and desorption of the proteins using sorbents with various contents of ionogenic groups allows to conclude, that the effective processes of ion-exchange chromatography can be realized only provided the competitive mechanism of sorption.
Demin
A A
AA
Institute of Macromolecular Compounds, Russian Academy of Science, Bolshoi Pr. 31, St. Petersburg, 199004, Russia.
Melenevsky
A T
AT
Papukova
K P
KP
D000327:Adsorption; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D007477:Ions; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D000837:Anion Exchange Resins; D007477:Ions; D011506:Proteins