Production of FaeG, the major subunit of K88 fimbriae, in transgenic tobacco plants and its immunogenicity in mice.
Transgenic tobacco plants stably expressing recombinant FaeG, which is the major subunit and adhesin of K88ad fimbriae, were obtained. Analysis of sera from immunized mice indicates that in mice, the immunogenicity induced by plant-derived FaeG protein is comparable to that generated with traditional approaches.
Huang
Yahong
Y
The Department of Biological Science and Technology, School of Life Science of Nanjing University, Nanjing. People's Republic of China.
Liang
Wanqi
W
Pan
Aihu
A
Zhou
Zhiai
Z
Huang
Cheng
C
Chen
Jianxiu
J
Zhang
Dabing
D
D018830:Adhesins, Escherichia coli / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005260:Female; D036781:Fimbriae Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D051379:Mice; D030821:Plants, Genetically Modified; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000235:genetics*
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D021122:Protein Subunits; D011994:Recombinant Proteins; D036781:Fimbriae Proteins