Topology of RbsC, the membrane component of the Escherichia coli ribose transporter.
The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli, has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC.
Stewart
Jeffrey B
JB
Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, IN 49707, USA.
Hermodson
Mark A
MA
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D012266:Ribose / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; C102054:RbsC protein, E coli; D012266:Ribose; D003545:Cysteine; D019793:Fluorescein