Biodegradation of polyglycolic acid-collagen composite tubes for nerve guide in the peritoneal cavity.
The rate of biodegradation of new types of polyglycolic acid (PGA)-collagen composite tubes for nerve regeneration was evaluated in the peritoneal cavity. PGA mesh tubes with a diameter of 2 or 4 mm were coated with collagen solution and dried at room temperature. The tubes were then subjected to dehydrothermal treatment (composite tube). A 2 mm PGA-collagen composite tube filled with collagen sponge was also investigated in this study (sponge tube). Tubes with a length of 15 mm were fixed at the parietal peritoneum of BALB/c mice and excised 2 weeks and 1, 2, and 3 months after the operation. The inner areas of the excised tubes were measured microscopically. Statistical analysis was performed by one way ANOVA and Fisher's PLSD test. Although the inner areas of the 2 and 4 mm composite tubes were not maintained 1 month after the operation (62 +/- 6.8% and 21 +/- 3.8%, respectively), they were well maintained in the sponge tubes (83 +/- 6.4%). The inner areas of the sponge tubes were significantly larger than those of the composite tubes until 2 months after surgery. These results suggest that sponge tubes are more suitable than composite tubes for nerve regeneration in the peritoneal cavity.
Ito
Tadao
T
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Nakamura
Tatsuo
T
Takagi
Tsuyoshi
T
Toba
Toshinari
T
Hagiwara
Akeo
A
Yamagishi
Hisakazu
H
Shimizu
Yasuhiko
Y
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D003094:Collagen; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000502:physiology; D010529:Peritoneal Cavity; D011100:Polyglycolic Acid; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation
D001672:Biocompatible Materials; D011100:Polyglycolic Acid; D003094:Collagen