[Use of polymerase chain reaction for determining T-cell clonality].
OBJECTIVE
To distinguish T-cell lymphomas and reactive T-cell proliferation it is important to confirm the ability of T-cells to be cloned. Conventional histological and immunophenotypic methods fail to determine the ability of T-cells to be cloned. An experience in the use of detection of T-cell receptor gene gamma-chain (TCRy) rearrangement for determining T-cellular clonality is described.


METHODS
Polymerase chain reaction (PCR) and single strand conformational polymorphism (SSCP) were used to determine T-cell clonality. Twenty healthy donors, 28 patients with T-lymphomas, and 26 patients with various non-T-cell lymphoproliferative disorders or reactive processes were studied.


RESULTS
T-cell monoclonality was detected in 23/28 (82%) T-cell lymphoma cases, whereas in all the samples from normal subjects a polyclonal pattern of rearrangements TCRy was found. The sensitivity of the method was estimated as 2.5%, 7%, and 10% was demonstrated for bone marrow, spleen, and peripheral blood, respectively.


CONCLUSIONS
PCR-SSCP for TCRy was found to be a useful supplement to routine histological and immunophenotypic methods in the diagnosis of T-cell lymphomas.
Sidorova
Iu V
IuV
Nikitin
E A
EA
Peklo
M
M
Vlasik
T N
TN
Samoĭlova
R S
RS
Kravchenko
S K
SK
Melikian
A L
AL
Vinogradova
Iu E
IuE
Pivnik
A V
AV
Sudarikov
A B
AB
D002999:Clone Cells / Q000473:pathology; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D016393:Lymphoma, B-Cell / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000473:pathology*
D017931:DNA Primers; D004247:DNA