title: Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development.
abstract: Fibroblast growth factor (Fgf) signaling plays an important role during development of posterior mesoderm in vertebrate embryos. Blocking Fgf signaling by expressing a dominant-negative Fgf receptor inhibits posterior mesoderm development. In mice, Fgf8 appears to be the principal ligand required for mesodermal development, as mouse Fgf8 mutants do not form mesoderm. In zebrafish, Fgf8 is encoded by the acerebellar locus, and, similar to its mouse otholog, is expressed in early mesodermal precursors during gastrulation. However, zebrafish fgf8 mutants have only mild defects in posterior mesodermal development, suggesting that it is not the only Fgf ligand involved in the development of this tissue. We report here the identification of an fgf8-related gene in zebrafish, fgf24, that is co-expressed with fgf8 in mesodermal precursors during gastrulation. Using morpholino-based gene inactivation, we have analyzed the function of fgf24 during development. We found that inhibiting fgf24 function alone has no affect on the formation of posterior mesoderm. Conversely, inhibiting fgf24 function in embryos mutant for fgf8 blocks the formation of most posterior mesoderm. Thus, fgf8 and fgf24 are together required to promote posterior mesodermal development. We provide both phenotypic and genetic evidence that these Fgf signaling components interact with no tail and spadetail, two zebrafish T-box transcription factors that are required for the development of all posterior mesoderm. Last, we show that fgf24 is expressed in early fin bud mesenchyme and that inhibiting fgf24 function results in viable fish that lack pectoral fins.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA. draper@fred.fhcrc.org
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Draper
authors.forename: David W
authors.initials: DW
authors.lastname: Stock
authors.forename: Charles B
authors.initials: CB
authors.lastname: Kimmel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004742:Enhancer Elements, Genetic; D005326:Fetal Proteins; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005775:Gastrula / Q000166:cytology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008024:Ligands; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000378:metabolism; D015027:Zebrafish / Q000033:anatomy & histology / Q000196:embryology* / Q000502:physiology; D029961:Zebrafish Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000098908:Brachyury Protein
chemical_list: D005326:Fetal Proteins; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D008024:Ligands; D016376:Oligonucleotides, Antisense; D020825:T-Box Domain Proteins; D029961:Zebrafish Proteins; D000098908:Brachyury Protein; C479568:fgf18a protein, zebrafish; C479567:fibroblast growth factor 24, zebrafish; C112539:tbx16 protein, zebrafish