Crucial roles of Brn1 in distal tubule formation and function in mouse kidney.
This study identifies a role for the gene for the POU transcription factor Brn1 in distal tubule formation and function in the mammalian kidney. Normal development of Henle's loop (HL), the distal convoluted tubule and the macula densa was severely retarded in Brn1-deficient mice. In particular, elongation and differentiation of the developing HL was affected. In the adult kidney, Brn1 was detected only in the thick ascending limb (TAL) of HL. In addition, the expression of a number of TAL-specific genes was reduced in the Brn1+/- kidney, including Umod, Nkcc2/Slc12a1, Bsnd, Kcnj1 and Ptger3. These results suggest that Brn1 is essential for both the development and function of the nephron in the kidney.
Nakai
Shigeyasu
S
Department of Cell Biology, Japanese Foundation for Cancer Research Cancer Institute, 1-37-1 Kami-Ikebukuro, Toshima-Ku, Tokyo 170-8455, Japan.
Sugitani
Yoshinobu
Y
Sato
Hiroshi
H
Ito
Sadayoshi
S
Miura
Yukio
Y
Ogawa
Masaharu
M
Nishi
Miyuki
M
Jishage
Kou-ichi
K
Minowa
Osamu
O
Noda
Tetsuo
T
D000818:Animals; D000831:Animals, Newborn; D018118:Chloride Channels; D005260:Female; D018628:Gene Dosage; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009088:Mucoproteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D050809:POU Domain Factors; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying; D011247:Pregnancy; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000378:metabolism; D058308:Receptors, Prostaglandin E, EP3 Subtype; D051437:Renal Insufficiency; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics / Q000378:metabolism; D064466:Solute Carrier Family 12, Member 1; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D058949:Uromodulin
C486687:Bsnd protein, mouse; D018118:Chloride Channels; C510693:Kcnj1 protein, mouse; D008565:Membrane Proteins; D009088:Mucoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D050809:POU Domain Factors; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; C545648:Ptger3 protein, mouse; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype; C578715:Slc12a1 protein, mouse; D028021:Sodium-Potassium-Chloride Symporters; D064466:Solute Carrier Family 12, Member 1; D015534:Trans-Activators; C548613:Umod protein, mouse; D058949:Uromodulin; C074065:Pou3f3 protein, mouse