Solubilization and refolding of inclusion body proteins in reverse micelles.
Today, many valuable proteins can be obtained in sufficient amounts using recombinant DNA techniques. However, frequently the expression of recombinant proteins results in the accumulation of the product in dense amorphous deposits inside the cells, called inclusion bodies. The challenge then is to transform these inactive and misfolded protein aggregates into soluble bioactive forms. Although a number of general guidelines have been proposed, the search for proper reconstitution conditions can be very laborious and time consuming. Here, we suggest a new versatile approach for solubilization and refolding of inclusion body proteins using a water-sodium bis-2-ethylhexyl sulfosuccinate-isooctane reverse micellar system. Instead of amorphous aggregates, a transparent solution is obtained, where refolded protein is entrapped inside the micelles. The entrapped enzyme has native-like secondary structure and catalytic activity. This approach has been implemented with Fusarium galactose oxidase and Stigmatella aurantiaca putative galactose oxidase.
Vinogradov
Alexei A
AA
Department of Chemical Enzymology, M.V. Lomonosov Moscow State University, Vorobievy gory, 119899 Moscow, Russia.
Kudryashova
Elena V
EV
Levashov
Andrei V
AV
van Dongen
Walter M A M
WM
D002942:Circular Dichroism; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism*; D008823:Micelles; D020673:Protein Renaturation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D020606:Stigmatella aurantiaca / Q000737:chemistry / Q000378:metabolism
D008823:Micelles; D011506:Proteins