Apoptosis and clonogenic survival in three tumour cell lines exposed to gamma rays or chemical genotoxic agents.
We compared the extent to which apoptosis is induced and clonogenicity reduced in three tumour cell lines - the human melanoma Me45 and promyelocytic leukaemia HL-60, and the rat rhabdomyosarcoma R1 - after exposure to the anticancer drugs etoposide and cis-platinum or to gamma radiation; each induces different types of DNA damage. Cells which readily underwent apoptosis did not necessarily show a correlated loss of clonogenicity; for example, Me45 cells showed the highest sensitivity to all three agents in clonogenic assays but much lower levels of apoptotic cells than R1 or HL-60 cells. These results show that the efficiency of the eradication of clonogenic cells by genotoxic agents does not solely depend on the induction of apoptotic processes, and suggest that the induction of apoptosis and suppression of clonogenicity are independent processes.
Kumala
Sławomir
S
Department of Experimental and Clinical Radiobiology, Center of Oncology, Wybrzeze AK 15, 44-100 Gliwice, Poland.
Niemiec
Paweł
P
Wideł
Maria
M
Hancock
Ronald
R
Rzeszowska-Wolny
Joanna
J
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D002945:Cisplatin / Q000633:toxicity; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005047:Etoposide / Q000633:toxicity; D005720:Gamma Rays; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000473:pathology; D015162:Micronucleus Tests; D051381:Rats; D012208:Rhabdomyosarcoma / Q000473:pathology
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D005047:Etoposide; D002945:Cisplatin