title: Sensitive determination of pentazocine in human tissues by high-performance liquid chromatography.
abstract: The authors developed a simple, reliable and sensitive method for the determination of pentazocine in human solid tissues using high-performance liquid chromatography, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as the internal standard. The extract was evaporated to dryness and dissolved in the mobile phase of acetonitrile/10 mM phosphate buffer (pH 4.0). The eluent was pumped at a flow rate of 0.4 ml/min through a Spherisorb Ph (2.1 mm I.D. x 150 mm) column. A fluorescence detector with excitation at 247 nm and emission at 320 nm was used. The lower limit of detection was about 0.5 ng/g. The calibration curve was linear over the concentration ranges from 1 to 500 ng/g in each tissue examined and could be determined up to at least 10.0 microg/g by means of reduction of injected volumes. Using this method, the concentrations of pentazocine could be determined in the tissues of an autopsied individual for toxicological evaluation.
authors.affiliation: Department of Legal Medicine, Hiroshima University School of Medicine, Hiroshima 734-8551, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Kudo
authors.forename: A
authors.initials: A
authors.lastname: Namera
authors.forename: M
authors.initials: M
authors.lastname: Yashiki
authors.forename: T
authors.initials: T
authors.lastname: Kojima