title: Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction.
abstract: The MALDI-TOF spectra of peptides from the sera of normal and myocardial infarction patients produced patterns that provided an accurate diagnostic of MI. In myocardial infarction, the spectral pattern originated from the cleavage of complement C3 alpha chain to release the C3f peptide and cleavage of fibrinogen to release peptide A. The fibrinogen peptide A and complement C3f peptide were in turn progressively truncated by aminopeptidases to produce two families of fragments that formed the characteristic spectral pattern of MI. Time course and inhibitor studies demonstrated that the peptide patterns in the serum reflect the balance of disease-specific-protease and aminopeptidase activity ex vivo.
authors.affiliation: SYNX PHARMA, 1 Marmac Drive, Toronto, Ontario, Canada M9W 1E7. jmarshall@synxpharma.com
authors.forename: John
authors.initials: J
authors.lastname: Marshall
authors.forename: Peter
authors.initials: P
authors.lastname: Kupchak
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.forename: Jason
authors.initials: J
authors.lastname: Yantha
authors.forename: Tammy
authors.initials: T
authors.lastname: Vrees
authors.forename: Shirley
authors.initials: S
authors.lastname: Furesz
authors.forename: Kellie
authors.initials: K
authors.lastname: Jacks
authors.forename: Chris
authors.initials: C
authors.lastname: Smith
authors.forename: Inga
authors.initials: I
authors.lastname: Kireeva
authors.forename: Rulin
authors.initials: R
authors.lastname: Zhang
authors.forename: Miyoko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Eric
authors.initials: E
authors.lastname: Stanton
authors.forename: George
authors.initials: G
authors.lastname: Jackowski
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D003176:Complement C3 / Q000378:metabolism; D003179:Complement C3b / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D003627:Data Interpretation, Statistical; D030541:Databases, Genetic; D001330:Electronic Data Processing / Q000379:methods; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D010447:Peptide Hydrolases / Q000187:drug effects / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D003176:Complement C3; D011480:Protease Inhibitors; C046821:complement C3f; C464794:fibrinogen Aalpha; D010664:Phenylmethylsulfonyl Fluoride; D003179:Complement C3b; D005340:Fibrinogen; D010447:Peptide Hydrolases