title: Detection of a metallo-beta-lactamase (IMP-1) by fluorescent probes having dansyl and thiol groups.
abstract: A fluorescent probe for the detection of a metallo-beta-lactamase (IMP-1), N-[2-(5-dimethylaminonaphthalen-1-ylsulfonylamino)ethyl]-3- mercaptopropionamide (Dansyl-C2SH), 1, was designed based on combining the inhibitory function of mercaptocarboxylate and a fluorophore. The binding of 1 to IMP-1 was investigated by fluorescence spectroscopy. Compound 1 can act as fluorescent probe for detecting IMP-1 selectively.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kumamoto University, Oe-honmachi 5-1, Kumamoto 862-0973, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Kurosaki
authors.forename: Hisami
authors.initials: H
authors.lastname: Yasuzawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Wanchun
authors.initials: W
authors.lastname: Jin
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Goto
mesh_terms: D002264:Carboxylic Acids / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008956:Models, Chemical; D013050:Spectrometry, Fluorescence; D001618:beta-Lactamases / Q000032:analysis* / Q000737:chemistry*
chemical_list: D002264:Carboxylic Acids; D005456:Fluorescent Dyes; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases