Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media.
Monodisperse magnetic nanoparticles conjugated with virus-surface-specific antibodies self-assemble in the presence of specific viral particles to create supramolecular structures with enhanced magnetic properties, as detected by magnetic resonance methods (NMR/MRI). The observed magnetic relaxation changes that occur upon viral-induced assembly allowed for highly sensitive and selective detection of a virus in complex biological media. The developed method was shown to specifically detect adenovirus-5 and herpes simplex virus-1 at concentrations of 5 viral particles/10 muL without the need of extensive sample preparation. The applications of this new method span from high-throughput NMR detection of viruses in biological samples to potential MR imaging of viral distribution in vivo.
Perez
J Manuel
JM
MGH-Center for Molecular Imaging Research, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA. perez@helix.mgh.harvard.edu
Simeone
F Joseph
FJ
Saeki
Yoshinaga
Y
Josephson
Lee
L
Weissleder
Ralph
R
D000256:Adenoviridae / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D003911:Dextrans / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008280:Magnetics; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000737:chemistry; D010316:Particle Size; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D003911:Dextrans; C029152:G-substrate; D009419:Nerve Tissue Proteins