Production of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine.
Murine monoclonal anti-human deoxyribonuclease II (DNase II) antibodies were obtained from BALB/c mice immunized with human DNase II purified from human liver. Both single radial enzyme diffusion and DNA-cast polyacrylamide gel electrophoresis were very useful screening methods for obtaining the DNase II-specific antibodies. All of the antibodies showed specific inhibition of human DNase II enzyme activity and specific immunostaining. Insertion of the immunoaffinity step in our purification procedure made the purification of human DNase II easier, faster and more effective than the conventional procedure.
Nakajima
Tamiko
T
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Gunma, 371-8511, Japan.
Yasuda
Toshihiro
T
Takeshita
Haruo
H
Mogi
Kouichi
K
Kaneko
Yasushi
Y
Kishi
Koichiro
K
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014556:Urine / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II