title: In vitro osteogenic differentiation of human ES cells.
abstract: Since their isolation in 1998, human embryonic stem (hES) cells have been shown to be capable of adopting various cell fates in vitro. Here, we present in vitro data demonstrating the directed commitment of human embryonic stem cells to the osteogenic lineage. Human ES cells are shown to respond to factors that promote osteogenesis, leading to activation of the osteogenic markers osteocalcin, parathyroid hormone receptor, bone sialoprotein, osteopontin, cbfa1, and collagen 1. Moreover, the mineralized nodules obtained are composed of hydroxyapatite, further establishing the similarity of osteoblasts in culture to bone. These results show that osteoblasts can be derived from human ES cultures in vitro and provide the basis for comparison of adult and embryonic-derived osteogenesis, and for an investigation of potential applications for hES cells in orthopaedic tissue repair.
authors.affiliation: Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, United Kingdom.
authors.forename: Virginie
authors.initials: V
authors.lastname: Sottile
authors.forename: Alison
authors.initials: A
authors.lastname: Thomson
authors.forename: Jim
authors.initials: J
authors.lastname: McWhir
mesh_terms: D000328:Adult; D001842:Bone and Bones; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D017886:Durapatite / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D013234:Stem Cells
chemical_list: D017077:Culture Media, Conditioned; D017886:Durapatite