[A new chemiluminescence immunoassay method for the determination of cancer embryo antigen].
A new chemiluminescent probe 10,10'-dimethyl-3,3'-disulfo-9,9'-biacridine DMDSBA) is used to label anti-CEA antibody, the molar incorporation is 1.15-1.32 mole of DMDSBA per mol anti-CEA; the conditions of labeling reaction, sandwich immunoassay and chemiluminescent reaction are studied. Moreover, a new double antibody sandwich chemiluminescence immunoassay is firstly established, the linear calibration ranges is 1.5-150.0 ng.mL-1 and the detection limit is 0.53 ng.mL-1. This method is used to detect cancer embryo antigen (CEA) in human serum, the results are well-matched with that of radio immunoassay.
Zhuang
H
H
Department of Chemistry, Fuzhou University, 350002 Fuzhou.
Wang
Q
Q
Chen
G
G
Huang
J
J
Lin
P
P
D002272:Carcinoembryonic Antigen / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity
D002272:Carcinoembryonic Antigen