Synthesis and hydrolysis studies of a peptide containing the reactive triad of serine proteases with an associated linker to a dye on a solid phase support.
The synthesis of a Tentagel-supported peptide incorporating the reactive triad of serine, histidine and aspartic acid, found within serine protease enzymes, is described.
Clough
John M
JM
Syngenta, Jealott's Hill Research Centre, Bracknell, Berkshire, UK RG42 6EY.
Jones
Ray V
RV
McCann
Hannah
H
Morris
David J
DJ
Wills
Martin
M
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000737:chemistry / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004950:Esterases / Q000737:chemistry; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008863:Microspheres; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D012694:Serine / Q000737:chemistry / Q000378:metabolism; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism
D004396:Coloring Agents; D009842:Oligopeptides; D001224:Aspartic Acid; D012694:Serine; D006639:Histidine; D004950:Esterases; D012697:Serine Endopeptidases