title: Mechanosensitive cation channels in arterial smooth muscle cells are activated by diacylglycerol and inhibited by phospholipase C inhibitor.
abstract: Mechanosensitive cation channels may be involved in the development of the myogenic tone of arteries. The molecular identity of these channels is not clear, but transient receptor potential channels (TRPCs) are good candidates. In the present study, we searched for mechanosensitive channels at the single-channel level in arterial smooth muscle cells using the patch-clamp technique and investigated the channel properties in the light of properties of TRPCs. With 140 mmol/L CsCl in the pipette solution, application of negative pressures to the back of the pipette induced the activation of channels the open probability of which increased with the amount of negative pressure. The current-voltage relationship was linear in symmetrical ionic conditions, and the single-channel conductances for Cs+, K+, and Na+ were 30, 36, and 27 pS, respectively. When NMDG+ was substituted for Cs+ in the pipette solution, inward currents were abolished, whereas outward currents remained active, indicating that the channels were nonselective to cations. The channel activity was blocked by intracellular Gd3+ and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid and increased by diacylglycerol and by cyclopiazonic acid. Phospholipase C inhibitor (U73122) inhibited not only channel activity but also the development of myogenic tone induced by stretching of the basilar arteries. These results suggest that the ion channel responsible for the development of myogenic tone is the 30-pS mechanosensitive cation channel that exhibits properties similar to those of TRPCs.
authors.affiliation: Department of Physiology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Kyoung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Yangmi
authors.initials: Y
authors.lastname: Kim
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Yung E
authors.initials: YE
authors.lastname: Earm
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Ho
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000166:cytology / Q000502:physiology*; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology*; D004553:Electric Conductivity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology; D005260:Female; D005682:Gadolinium / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007473:Ion Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D009119:Muscle Contraction; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011760:Pyrrolidinones / Q000494:pharmacology; D011817:Rabbits; D013314:Stress, Mechanical; D019284:Thapsigargin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
chemical_list: D002412:Cations; D004075:Diglycerides; D004791:Enzyme Inhibitors; D004963:Estrenes; D007211:Indoles; D007473:Ion Channels; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D019284:Thapsigargin; D005682:Gadolinium; D010738:Type C Phospholipases; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; C000543:cyclopiazonic acid