Optical sectioning by two-pinhole confocal fluorescence microscopy.
A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.
Martínez-Corral
M
M
Departamento de Optica, Universidad de Valencia, Burjassot 46100, Spain. manuel.martinez@uv.es
Caballero
M T
MT
Ibáñez-López
C
C
Sarafis
V
V
D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D013382:Subtraction Technique / Q000295:instrumentation*