title: Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA analysis.
abstract: Isoenzymes and RAPD (random amplified polymorphic DNA) analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be related to the clonal reproduction of this protozoan.
authors.affiliation: School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. morocha@farmacia.ufmg.br
authors.forename: Míriam O
authors.initials: MO
authors.lastname: Rocha
authors.forename: Maria A
authors.initials: MA
authors.lastname: Gomes
authors.forename: Adriana O
authors.initials: AO
authors.lastname: Costa
authors.forename: Cinthia
authors.initials: C
authors.lastname: Furst
authors.forename: Edward F
authors.initials: EF
authors.lastname: Silva
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis*; D004592:Electrophoresis, Starch Gel; D019353:Endemic Diseases; D005260:Female; D016829:Giardia lamblia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005873:Giardiasis / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan; D007527:Isoenzymes