[Effect of triamcinolone acetonide on the proliferation and monocyte chemoattractant protein expression in rat glomerular mesangial cells].
OBJECTIVE
To explore the inhibitory effect and mechanism of triamcinolone acetonide (TA) on the proliferation and monocyte chemoattractant protein-1(MCP-1) expression in rat glomerular mesangial cells (GMCs).


METHODS
TA with different concentrations was put in the cultured rat GMC line in vitro after Lipopolysaccharide (LPS). There were three groups in our experiment: the GMC group, LPS group (GMCs + LPS), and TA group(GMCs + LPS + TA). The GMC proliferation level was detected by the 3-(4,5-Dimethylthiazol-2-yl)2,5-diphenyltetrazo-lium bromid (MTT) incorporation at the 24th and 48th hours. The expression of MCP-1 and nuclear transcriptional factor-Kappa B (NF-kappa B) were checked by immunohistochemistry. The MCP-1 concentration was determined by ELISA.


RESULTS
1. In the TA group, the GMC proliferative level was obviously lower than that either in the LPS group or in the GMC group (P < 0.01). 2. The MCP-1 expression of GMCs in the TA group was obviously lower than that in any of the other groups (P < 0.01). 3. The NF-kappa B expression in GMCs in the TA group was significantly lower than that in the LPS group (P < 0.01), but there was no significant difference in the NF-kappa B expression between the TA group and LPS group (P > 0.05). 4. The concentration of MCP-1 in the TA group was obviously lower than that in the LPS group (P < 0.01), while there was no significant difference in the MCP-1 concentration between the TA group and the GMC group (P > 0.05).


CONCLUSIONS
TA can obviously inhibit the proliferation of GMCs, and down-regulate the abnormal expression and secretion of MCP-1 by reducing the activation of NF-kappa B.
Wu
Xiao-chuan
XC
Laboratory of Pediatric Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Yi
Zhu-wen
ZW
Xiao
Jian-wu
JW
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology; D008070:Lipopolysaccharides; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D014222:Triamcinolone Acetonide / Q000494:pharmacology*
D018932:Chemokine CCL2; D005938:Glucocorticoids; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014222:Triamcinolone Acetonide