Determination of proteins using the p-acetylchlorophosphonazo-barium(II) complex as a spectroprobe.
A novel spectrophotometric method has been developed for the determination of proteins using the p-acetychlorophosphonazo (CPA-pA)-barium(II) complex as a spectroprobe. In the pH range of 2.0-2.8, the absorbance of the CPA-pA-barium(II) complex at 649 nm is greatly decreased by protein. The absorbance decrease is in proportion to the concentration of protein in the range of 0-20 mg/L. The apparent molar absorptivities are 1.56 x 10(6), 1.70 x 10(6) and 6.04 x 10(5) L mol(-1) cm(-1) for bovine serum albumin (BSA), human serum albumin (HAS) and ovalbumin (OVA), respectively. The method is reproducible and simple, and has been used to determine total protein in human sera. The results are in agreement with those obtained by the pyrocatechol violet (PV)-Mo(VI) method, with relative standard deviations of 2.5-3.5% (n=6).
Zhao
Shulin
S
Department of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.
Liu
Dianmei
D
Li
Shuting
S
D001464:Barium / Q000737:chemistry; D002021:Buffers; D002138:Calibration; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009942:Organometallic Compounds / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D002021:Buffers; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D011506:Proteins; D013501:Surface-Active Agents; C484402:p-acetylchlorophosphonazo-barium; D001464:Barium; D000431:Ethanol