[Effects of bcr/abl fusion gene on expression of beta1 integrin and L-selectin in mouse chronic myeloid leukemia cells].
OBJECTIVE
To explore the effects of p210 bcr/abl fusion gene on expression of beta1 integrin and L-selectin mRNAs in mouse chronic myeloid leukemia (CML) cells.


METHODS
Comparisons of beta1 integrin and L-selectin mRNA levels among p210 bcr/abl negative, p210 bcr/abl positive, and p210 bcr/abl-Rb-C-Box positive cells were undertaken by quantity RT-PCR.


RESULTS
In p210 bcr/abl positive cells, L-selectin mRNA level was decreased, but beta1 integrin mRNA expression had no change as compared to those in p210 bcr/abl negative cells. When inhibition of bcr-abl tyrosine kinase activity by Rb-C-Box, the L-selectin mRNA expression restored to normal (similar to p210 bcr/abl negative cells).


CONCLUSIONS
p210 bcr/abl oncoprotein inhibits expression of L-selectin mRNA, but not of beta1 integrin mRNA.
Wang
Wei-liang
WL
Department of Hematology, Beijing Hospital, Beijing 100730, China.
Shen
Ti
T
Hui
Yu-rong
YR
Gu
Xi-chun
XC
Li
Rong-sheng
RS
D000818:Animals; D045744:Cell Line, Tumor; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D016161:Genes, Retinoblastoma / Q000235:genetics; D019012:Integrin beta1 / Q000235:genetics*; D019041:L-Selectin / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D019012:Integrin beta1; D012333:RNA, Messenger; D019041:L-Selectin; D016044:Fusion Proteins, bcr-abl