[Interfering effects of radix Salviae miltiorrhizae and lingustrazine on mm-LDL activating BKCa in ECV304 cell].
OBJECTIVE
To observe the action of minimally modified low density lipoprotein (mm-LDL) on BKCa in ECV304 cell and the interfering effects of radix salviae miltiorrhizae extract powder 764-3 (30 micrograms/ml) and lingustrazine (200 micrograms/ml) on this action.


METHODS
The cell-attached configuration of patch clamp technique was applied.


RESULTS
mm-LDL (100 micrograms/ml) potentiated the activity of BKCa in ECV304. While 764-3 and lingustrazine abolished it.


CONCLUSIONS
mm-LDL acted on vascular endothelial cell ECV304 could rapidly activate the activity of BKCa and might result in the increase of electro-chemical gradient for the resting Ca2+ influx, thus resting cytoplasmic concentration of calcium could be elevated and endothelial dysfunction would be induced. 764-3 and lingustrazine might have the protective action through decreasing the activity of BKCa.
Lin
L
L
Department of Physiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zheng
Y F
YF
Qu
J H
JH
Bao
G H
GH
D002478:Cells, Cultured; D004336:Drug Antagonism; D006801:Humans; D030004:Ligusticum; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology*; D024681:Potassium Channels, Calcium-Activated / Q000187:drug effects*; D027561:Salvia miltiorrhiza; D014471:Umbilical Veins / Q000166:cytology
D008077:Lipoproteins, LDL; D028321:Plant Preparations; D024681:Potassium Channels, Calcium-Activated; C099252:oxidized low density lipoprotein