Characterization of a small cryptic plasmid, pHP51, from a Korean isolate of strain 51 of Helicobacter pylori.
The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because it lacked known functional domains or sequence similarity with other known proteins, although it had a HPFXXGNG motif that was also found in the cAMP-induced filamentation (fic) gene. Three kinds of repeats were present on the plasmid outside of the ORFs, including the R1 and R2 repeats that are common in H. pylori plasmids. One 100-bp sequence detected in the noncoding region of pHP51 was highly similar to the genomic sequence of H. pylori 26695.
Song
Jae-Young
JY
Department of Microbiology, Gyeongsang Institute of Health Science, Gyeongsang National University College of Medicine, 90 Chiram-dong, Jinju, Gyeongsangnam-do 660-751, South Korea.
Choi
Sang-Haeng
SH
Byun
Eun-Young
EY
Lee
Seung-Gyu
SG
Park
Ye-Hyoung
YH
Park
Seong-Gyu
SG
Lee
Sun-kyung
SK
Kim
Kyung-Mi
KM
Park
Jeong-Uck
JU
Kang
Hyung-Lyun
HL
Baik
Seung-Chul
SC
Lee
Woo-Kon
WK
Cho
Myung-Je
MJ
Youn
Hee-Shang
HS
Ko
Gyung-Hyuck
GH
Bae
Dong-Won
DW
Rhee
Kwang-Ho
KH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004265:DNA Helicases / Q000235:genetics; D004268:DNA-Binding Proteins; D016480:Helicobacter pylori / Q000235:genetics*; D007723:Korea; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases