title: Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.
abstract: OBJECTIVE
  To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.
  
  
  METHODS
  Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.
  
  
  RESULTS
  Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.
  
  
  CONCLUSIONS
  siRNA may be effective in inhibiting SARS-associated coronavirus replication.
authors.affiliation: Sun Yat-Sen University, Guangzhou 510089, China.
authors.forename: Renli
authors.initials: R
authors.lastname: Zhang
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Guo
authors.forename: Jiahai
authors.initials: J
authors.lastname: Lu
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Meng
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
authors.forename: Ximei
authors.initials: X
authors.lastname: Zhan
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Xinghua
authors.initials: X
authors.lastname: Pan
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ling
authors.forename: Xigu
authors.initials: X
authors.lastname: Chen
authors.forename: Zhuoyue
authors.initials: Z
authors.lastname: Wan
authors.forename: Huanying
authors.initials: H
authors.lastname: Zheng
authors.forename: Xinge
authors.initials: X
authors.lastname: Yan
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Ran
authors.forename: Xinjian
authors.initials: X
authors.lastname: Liu
authors.forename: Junxin
authors.initials: J
authors.lastname: Ma
authors.forename: Chengyu
authors.initials: C
authors.lastname: Wang
authors.forename: Biliang
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D034741:RNA, Small Interfering / Q000494:pharmacology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects*; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D034741:RNA, Small Interfering