title: Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction.
abstract: We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikatacho Okayama city, Japan. inagaki@md.okayama-u.ac.jp
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Inagaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Doi
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Takata
authors.forename: Takaki
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Kei
authors.initials: K
authors.lastname: Yoshitome
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyaishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Ishizu