A dual binding site for integration host factor and the response regulator CtrA inside the Caulobacter crescentus replication origin.
The response regulator CtrA controls chromosome replication by binding to five sites, a, b, c, d, and e, inside the Caulobacter crescentus replication origin (Cori). In this study, we demonstrate that integration host factor (IHF) binds Cori over the central CtrA binding site c. Surprisingly, IHF and CtrA share DNA recognition sequences. Rather than promoting cooperative binding, IHF binding hinders CtrA binding to site c and nearby site d. Unlike other CtrA binding sites, DNA mutations in the CtrA c/IHF site uniquely impair autonomous Cori plasmid replication. These mutations also alter transcription from distant promoters more than 100 bp away. When the CtrA c/IHF site was deleted from the chromosome, these cells grew slowly and became selectively intolerant to a CtrA phosphor-mimic allele (D51E). Since CtrA protein concentration decreases during the cell cycle as IHF protein concentration increases, we propose a model in which IHF displaces CtrA in order to bend Cori and promote efficient chromosome replication.
Siam
Rania
R
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4.
Brassinga
Ann Karen C
AK
Marczynski
Gregory T
GT
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D016935:Caulobacter crescentus / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D034982:Integration Host Factors / Q000235:genetics / Q000378:metabolism*; D018716:Molecular Mimicry; D009154:Mutation; D010758:Phosphorus / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D018741:Replication Origin; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C097706:CtrA protein, Caulobacter; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D014157:Transcription Factors; D010758:Phosphorus