Elevated FXIIIA and serine proteases upon filtration of platelet concentrate on negatively charged filters: comparison with other established haemostatic markers of platelet activation and storage lesion.
Tests for the assessment of the platelet storage lesion are still evolving. To be of clinical value, they should reflect one of the physiological functions of platelets and should be simple, practical and fit in to the transfusion laboratory setting. In this respect, FXIIIA, Annexin V, vWF and glycocalicin (GC), measured by the established ELISA technology, are of particular relevance, as their roles in the maintenance of haemostatic balance are fully established and they can be released or cleaved upon platelet activation/injury/microvesiculation. The usefulness of these haemostatic markers for in process validation/process qualification, in particular subsequent to harsh procedures and when blood components come in contact with various artificial surfaces, is established and compared with a practical test for routine quality monitoring of platelet functional activity and aggregation states test.
Seghatchian
Jerard
J
Blood Component Technology & Thrombosis/Haemostasis Consultancy, 50 Primrose Hill Road, London NW3 3AA, UK. jseghatchian@btopenworld.com
D017304:Annexin A5 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D026122:Factor XIIIa / Q000378:metabolism*; D005374:Filtration / Q000295:instrumentation; D015539:Platelet Activation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis; D012697:Serine Endopeptidases / Q000378:metabolism*; D013997:Time Factors; D014841:von Willebrand Factor / Q000032:analysis
D017304:Annexin A5; D015415:Biomarkers; D019038:Platelet Glycoprotein GPIb-IX Complex; C023592:glycocalicin; D014841:von Willebrand Factor; D026122:Factor XIIIa; D012697:Serine Endopeptidases