[Cyclosporin A inhibits insulin release and down-regulates gene expressions of mitochondrial oxidative phosphorylation enzymes in NIT-cells].
OBJECTIVE
To investigate the effects of cyclosporin A on insulin release and the gene expression profiles of mitochondrial oxidative phosphorylation in NIT-1 cells.


METHODS
NIT-1 cells were exposed to cyclosporin A (10 micromol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.


RESULTS
Cyclosporin A reduced insulin release in the cell culture after 24 and 48 h exposure and decreased Nuox23, Cox7c and Atp5K mRNA expressions.


CONCLUSIONS
Cyclosporin A induces inhibition of insulin release in NIT-1 cells, possibly due to the reduction of ATP synthesis involving the down-regulation of the gene expression of mitochondrial oxidative phosphorylation enzymes.
Yu
Le
L
Institute of Pharmaceutic Science, First Military Medical University, Guangzhou 510515, China.
Lei
Lin-sheng
LS
Wu
Shu-guang
SG
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology*; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D010085:Oxidative Phosphorylation / Q000187:drug effects*
D007328:Insulin; D016572:Cyclosporine; D000255:Adenosine Triphosphate; D002118:Calcium