[Cloning of differentially displayed cDNAs involved in NaF-treated osteoblasts].
OBJECTIVE
To explore the effects of excessive fluoride on the gene expression of rat osteoblasts.


METHODS
Rat osteoblasts were treated with 2.0 mmol/L of sodium fluoride (NaF) for two weeks in vitro, and difference in the gene expression between the NaF-treated and normal osteoblasts was compared with mRNA differential display (DD-PCR) technique.


RESULTS
Among the six differentially expressed gene fragments which had been cloned, expression of the ribosomal protein L5 gene, ATPase Na(+)K(+) transporting beta polypeptide 3 gene, karyopherin alpha 2 gene and cis-Golgi body p28 gene was lower and expression of ubiquitous-conjugating enzyme E2D 3 gene and a newly-discovered gene fragment in this study showed up-regulated in the NaF-treated osteoblasts of the rats.


CONCLUSIONS
Expression of genes changed in the osteoblasts after treatment with fluoride for two weeks and most of them associated with synthesis, transportation and processing of protein. It suggested that excessive fluoride could affect the protein synthesis in osteoblasts by changing the expression of the related genes. A novel gene related to excessive fluoride exposure was also found.
Miao
Qing
Q
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu
Bing-ci
BC
Zhang
Xu-chao
XC
You
Bao-rong
BR
Xu
Ming
M
Kang
Ning
N
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005333:Fetus; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D012886:Skull / Q000473:pathology; D012969:Sodium Fluoride / Q000633:toxicity*
D018076:DNA, Complementary; D012969:Sodium Fluoride