Strand breaks in X-irradiated crystalline DNA: alternating CG oligomers.
Direct ionization of crystalline d(CGCGCGCG) and d(CGCGCGCGCG) oligomers produces 3'- and 5'-phosphate-terminated fragments as the main strand breakage products detectable by ion-exchange chromatography. The nature of the base has no effect on the probability of strand breakage at the given site. The yields of 3'-phosphates are systematically lower than the yields of the 5'-phosphates originating from the same cleavage site, pointing to the possible presence of unidentified products with sugar remnants attached to the 3'-end. These results show that direct ionization is efficient at producing single-strand breaks in DNA and its action is relatively indiscriminate with respect to base sequence.
Razskazovskiy
Yuriy
Y
Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York 14642, USA.
Debije
Michael G
MG
Howerton
Shelley B
SB
Williams
Loren D
LD
Bernhard
William A
WA
D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D007477:Ions; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects*; D014965:X-Rays
D007477:Ions; D009838:Oligodeoxyribonucleotides; D004247:DNA