A linkage map of meadow fescue ( Festuca pratensis Huds.) and comparative mapping with other Poaceae species.
A genetic linkage map has been constructed for meadow fescue ( Festuca pratensis Huds.) (2n=2 x=14) using a full-sib family of a cross between a genotype from a Norwegian population (HF2) and a genotype from a Yugoslavian cultivar (B14). The two-way pseudo-testcross procedure has been used to develop separate maps for each parent, as well as a combined map. A total number of 550 loci have been mapped using homologous and heterologous RFLPs, AFLPs, isozymes and SSRs. The combined map consists of 466 markers, has a total length of 658.8 cM with an average marker density of 1.4 cM/marker. A high degree of orthology and colinearity was observed between meadow fescue and the Triticeae genome(s) for all linkage groups, and the individual linkage groups were designated 1F-7F in accordance with the orthologous Triticeae chromosomes. As expected, the meadow fescue linkage groups were highly orthologous and co-linear with Lolium, and with oat, maize and sorghum, generally in the same manner as the Triticeae chromosomes. It was shown that the evolutionary 4AL/5AL translocation, which characterises some of the Triticeae species, is not present in the meadow fescue genome. A putative insertion of a segment orthologous to Triticeae 2 at the top of 6F, similar to the rearrangement found in the wheat B and the rye R genome, was also observed. In addition, chromosome 4F is completely orthologous to rice chromosome 3 in contrast to the Triticeae where this rice chromosome is distributed over homoeologous group 4 and 5 chromosomes. The meadow fescue genome thus has a more ancestral configuration than any of the Triticeae genomes. The extended meadow fescue map reported here provides the opportunity for beneficial cross-species transfer of genetic knowledge, particularly from the complete genome sequence of rice.
Alm
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Department of Chemistry and Biotechnology, Agricultural University of Norway, PO. Box 5040, 1432, As, Norway.
Fang
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C
Busso
C S
CS
Devos
K M
KM
Vollan
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Grieg
Z
Z
Rognli
O A
OA
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D002523:Edible Grain / Q000235:genetics; D019143:Evolution, Molecular; D031751:Festuca / Q000235:genetics*; D015321:Gene Rearrangement; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014178:Translocation, Genetic
D018744:DNA, Plant; D005819:Genetic Markers