H-NMR spin-lattice and correlation times of burned soft-tissue after treatment with an infrared pulsed laser device.
OBJECTIVE
The aim of this study was to investigate the spin-lattice (T(1), 1/T(1)) and correlation times (tau(c)) of burned soft-tissue after treatment with an infrared (IR) pulsed laser device (IPLD, 904 nm pulsed at 3 MHz).


METHODS
Seven groups (GI-GVII), each consisting of four albino rats, were used. Groups I-VI were anesthetized and burned with a hot tip: GI, GIII, GV were not irradiated; GII, GIV, GVI were irradiated at 0; 0 and 24; and 0, 24, and 48 hours, respectively. A control group (GVII) was neither burned nor irradiated. Samples from all groups were evaluated using a 90 MHz hydrogen nuclear magnetic resonance (H-NMR) spectrometer. An unpaired Student's t-test and an ANOVA I were preformed (alpha = 0.05).


RESULTS
At 0 and 24 hours, 1/T(1) and tau(c) data revealed significant differences between GVII and both the non-irradiated (GI, GIII), and irradiated (GII, GIV) groups. At 48 hours, only the difference in tau(c) between GVII and the irradiated group (GVI) remained significant.


CONCLUSIONS
Spin-lattice data reflected significant changes in tissues induced by the burn and a tendency towards control values for all burned groups. Meanwhile, the tau(c) value of GVI suggests the possibility of enhanced reparative effects attributable to chaotic intra- and inter-molecular energy transport to biopolymers in injured soft-tissue.
Rodríguez-Santana
Elizabeth
E
Fundalas, Foundation for Interdisciplinary Research and Development, Caracas, Venezuela. fundalals@mail2science.com
Santana-Blank
Luis A
LA
Reyes
Heberto
H
Santana-Rodríguez
Karin E
KE
Hunger
Manfred
M
Orellana
Rafael
R
Ortega
Daniel
D
D000818:Animals; D002056:Burns / Q000503:physiopathology* / Q000532:radiotherapy*; D004195:Disease Models, Animal; D007259:Infrared Rays / Q000627:therapeutic use*; D053685:Laser Therapy; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000503:physiopathology* / Q000528:radiation effects*; D019097:Spin Trapping; D013997:Time Factors