[Abnormal methylation of several tumor suppressor genes in sporadic breast cancer].
Multiplex methylation-sensitive PCR was employed in studying the methylation of CpG islands in the RB1, p16/CDKN2A, p15/CDKN2B, p14/ARF, CDH1, MGMT, HIC1, and N33 promoter regions in breast carcinoma (105 tumors). Methylation was often observed for the two major suppressor genes involved in cell-cycle control through the Cdk-Rb-E2F signaling pathway, RB1 (18/105, 17%) and p16 (59/105, 56%); both genes were methylated in 13 tumors. Methylation involved p15 in two (2%) tumors; CDH1, in 83 (79%) tumors; MGMT, in eight (8%) tumors, and N33, in nine (9%) tumors. The p14 promoter was not methylated in the tumors examined.
Zemliakova
V V
VV
Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia. valzem@mail.com
Zhevlova
A I
AI
Strel'nikov
V V
VV
Liubchenko
L N
LN
Vishnevskaia
Ia V
IaV
Tret'iakova
V A
VA
Zaletaev
D V
DV
Nemtsova
M V
MV
D001943:Breast Neoplasms / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D018899:CpG Islands; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics; D012016:Reference Values; D016160:Retinoblastoma Protein / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; C096870:Hic1 protein, mouse; D051741:Kruppel-Like Transcription Factors; D016160:Retinoblastoma Protein; D014157:Transcription Factors; D025803:Tumor Suppressor Protein p14ARF; D025521:Tumor Suppressor Proteins; D019853:O(6)-Methylguanine-DNA Methyltransferase