Expression of ARPP-16/19 in rat denervated skeletal muscle.
It is known that denervation of rat skeletal muscle causes atrophy and this is often adopted as a model for human muscle atrophy. To understand the molecular changes that occur, it is important to identify the profiles of differential gene expression. In the present study, we investigated differentially expressed genes in denervated muscle using DNA microarrays with printed genes preferentially expressed in skeletal muscle. We found that several genes are differentially expressed. Of these genes, ARPP-16/19 (cAMP-regulated phosphoprotein 16/19) is selectively enhanced after denervation. The expression of ARPP-16/19 in denervated muscles starts to increase from two days after denervation surgery. On the other hand, the expression of ARPP-16/19 does not change in hind-limb suspended muscles, such as EDL and soleus muscles. These results suggest that the increase in ARPP-16/19 mRNA expression is regulated by unknown factor(s) secreted from nerves, and not by electrical muscle activity.
Yoshikawa
Ayumu
A
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902.
Mitsuhashi
Hiroaki
H
Sasagawa
Noboru
N
Tsukahara
Toshifumi
T
Hayashi
Yukiko
Y
Nishino
Ichizo
I
Goto
Yu-ichi
Y
Ishiura
Shoichi
S
D000818:Animals; D015152:Blotting, Northern; D015870:Gene Expression; D006614:Hindlimb / Q000294:innervation / Q000378:metabolism; D007120:Immunochemistry; D008297:Male; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation* / Q000378:metabolism* / Q000648:ultrastructure; D009929:Organ Size; D010750:Phosphoproteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000601:surgery; D014158:Transcription, Genetic
D010750:Phosphoproteins; D012333:RNA, Messenger; C064322:cyclic AMP-regulated phosphoprotein 16; C064321:cyclic AMP-regulated phosphoprotein 19