[Effects of sevoflurane-induced and anoxia-induced preconditioning on HSP70 expression in neonatal rat cardiomyocytes].
OBJECTIVE
To explore the effects of sevoflurane-preconditioning and anoxia-preconditioning on HSP70 expression in neonatal rat cardiomyocytes.


METHODS
The second generation of primary cultured cardiomyocytes was randomly divided into 4 groups: normal control (Group C), anoxia/reoxygenation group (Group A/R), anoxia-preconditioning group (Group IP), and sevoflurane-preconditioning group (Group S); In each group, the cardiomyocytes were subjected to anoxia (2 h) followed by reoxygenation (48 h). The immunohistochemistry was used to determine the expression of HSP70 protein in neonatal rat cardiomyocytes at 0, 1, 12, 24, 36 and 48 h after reoxygenation respectively.


RESULTS
At each point of time studying reoxygenation, the expression level of HSP70 in Group S and Group IP was significantly higher than that in Group A/R and Group C (P < 0.01). The HSP70 expression level in Group A/R was slightly higher than that in Group C. No significant difference was found in HSP70 expression between Group IP and Group S (P > 0.05). The expression level of HSP70 protein in both Group S and Group IP increased concomitantly with the reoxygenation time from 1 h after reoxygenation, and reached the peak at 24 h of reoxygenation (P < 0.01).


CONCLUSIONS
sevoflurane-preconditioning and anoxia preconditioning can induce the high level expression of HSP70 in the neonatal rat cardiomyocytes with the phase of protection delayed. It is suggested that HSP70 may be involved in the process of the delayed phase of protection induced by sevoflurane-preconditioning and anoxia-preconditioning respectively.
Tang
Ying
Y
Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Wang
Quan-yun
QY
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008738:Methyl Ethers / Q000494:pharmacology*; D051379:Mice; D017202:Myocardial Ischemia / Q000139:chemically induced; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
D018840:HSP70 Heat-Shock Proteins; D008738:Methyl Ethers; D000077149:Sevoflurane