A simple pen-spotting method for arraying biomolecules on solid substrates.
We describe a simple, relatively inexpensive method for depositing biomolecules on a solid substrate using Rapidograph drafting pens. The pens can be used without modification to accurately deposit spots between approximately 100 and 600 microm in diameter. When mounted on a suitable microtranslation stage, the pens can be used to easily deposit tens of spots aligned with underlying substrate features such as microfabricated sensors. The pens are particularly convenient because pre-mixed solutions can be stored in the pens for multiple uses. We demonstrate the use of this approach to deposit DNA probes on a microsensor array.
Sheehan
P E
PE
Naval Research Laboratory, Washington, DC 20375-5342, USA. paul.sheehan@nrl.navy.mil
Edelstein
R L
RL
Tamanaha
C R
CR
Whitman
L J
LJ
D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*
D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D015335:Molecular Probes; D004247:DNA