[Improvement of osseointegration of titanium dental implants by a modified sandblasted surface].
OBJECTIVE
To study effects of the modified sandblasted surface of titanium implants, developed by authors, on the bone healing process.


METHODS
Osteoblasts were derived from the 5th passage of human fetal osteoblasts after primary isolation. Alkaline phosphatase (ALP) activities and protein contents of cellular layers, and osteocalcin contents in culturing medium were employed as criteria to evaluate osteogenic functions and differentiation of osteoblasts. The ALP activity was assayed utilizing the kinetic method, the protein content utilizing the Coomassie's method, and the osteocalcin content utilizing the radioimmune assay (RIA) method. Values of all criteria were divided by the corresponding cell numbers of different groups at a respective time point for the purpose of standardization. Samples were assigned to three groups-the modified sandblasted surface group, the smooth surface group and the blank control group, The culture was ended at, 4 days and 13 days.


RESULTS
At 4 days of culture, the modified sandblasted surface group showed a superiority to the smooth group with respects to the ALP activity [(17.390 +/- 1.595) nmol PNP x min(-1) x 10(-6) cells vs. (10.978 +/- 1.879) nmol PNP x min(-1) x 10(-6) cells] and protein content [(152.7 +/- 16.3) micro g/10(6) cells vs. (58.0 +/- 5.9) micro g/10(6) cells] of cellular layers and the osteocalcincontent [(43.0 +/- 6.1) ng/10(6) cells vs. (24.9 +/- 6.0) ng/10(6) cells] in culturing medium. Till the 13th day of culture, no differences were detected.


CONCLUSIONS
It is cytologically proved that the modified sandblasted surface can accelerate the bone healing process of implants though the improvement of osteoblastic functions and differentiation.
Li
De-hua
DH
Department of Implantation, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Liu
Bao-lin
BL
Song
Ying-liang
YL
Wu
Jun-zheng
JZ
D001861:Bone Regeneration; D002478:Cells, Cultured; D019250:Dental Implants, Single-Tooth; D005333:Fetus; D006801:Humans; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties; D014025:Titanium
D014025:Titanium