New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element.
Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1-4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain.
Tournier
Barthélémy
B
UMR 990 INRA/INP-ENSAT, Pole de Biotechnologie Végétale, 24 Chemin de Borde Rouge, P.O. Box 107, 31326 Castanet Tolosan Cedex, France.
Sanchez-Ballesta
Maria Theresa
MT
Jones
Brian
B
Pesquet
Edouard
E
Regad
Farid
F
Latché
Alain
A
Pech
Jean-Claude
JC
Bouzayen
Mondher
M
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D018744:DNA, Plant / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics; D009928:Organ Specificity; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011487:Protein Conformation; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D005030:Ethylenes; D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C093680:ethylene-responsive element binding protein; C036216:ethylene