title: Roles of TolC-dependent multidrug transporters of Escherichia coli in resistance to beta-lactams.
abstract: AcrAB exports some beta-lactam antibiotics in the periplasm out of cells via an outer-membrane channel, TolC. It has been reported that eight drug transporters in Escherichia coli cooperate with TolC. In this study, the roles of the drug exporters of E. coli in beta-lactam resistance were examined. We found that five of five resistance-nodulation-cell division-type drug exporters confer beta-lactam antibiotic resistance, while no other drug exporters confer any beta-lactam resistance even when they cooperate with TolC.
authors.affiliation: Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Nishino
authors.forename: Junko
authors.initials: J
authors.lastname: Yamada
authors.forename: Hidetada
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hirata
authors.forename: Akihito
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics* / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C073126:tolC protein, E coli