The stability of intramolecular DNA quadruplexes with extended loops forming inter- and intra-loop duplexes.
We have examined the formation of intramolecular quadruplex DNA structures in which the loops have been extended so as to generate short DNA duplexes. Fluorescence melting and DNase I cleavage studies show that duplexes can be formed within each loop, but that duplexes between the loops are not stable.
Risitano
Antonina
A
Division of Biochemistry & Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK SO16 7PX.
Fox
Keith R
KR
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry; D013696:Temperature
D009841:Oligonucleotides; D004247:DNA; D003850:Deoxyribonuclease I