Molecular epidemiology of human immunodeficiency virus-1 in the state of Ceará, Northeast, Brazil.
We analyzed, by env and gag heteroduplex mobility assay, 149 human immunodeficiency virus (HIV-1) positive samples collected in Ceará during the year 2000. The prevalence of subtype B was 81.2% and the prevalence of subtype F and B/F recombinants were both 2.7%. Eight (5.4%) and 12 (8%) out of 149 samples showed indeterminate results in the env and gag analysis respectively. By FokI restriction fragment length polymorphism, 34% of the subtype B samples were identified as the typical Brazilian subtype B. In the present study, we identified HIV-1 subtype F and B/F in Ceará for the first time. Our results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates.
Gadelha
Sandra Rocha
SR
Laboratório Avançado de Saúde Pública, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, 40295-001, Brasil.
Shindo
Nice
N
Cruz
José Napoleão Monte
JN
Morgado
Mariza Gonçalves
MG
Galvão-Castro
Bernardo
B
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D015752:Genes, env / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000453:epidemiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence
D004279:DNA, Viral