title: [Role of p38 pathway in PMA-induced in vitro invasion of JAR human choriocarcinoma cell line].
abstract: OBJECTIVE
  To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathways in regulating the in vitro invasion of JAR human choriocarcinoma cells induced by phorbol 12-myristate 13-acetate (PMA).
  
  
  METHODS
  ELISA was used to detect the kinase activity of the JAR cells in response to PMA stimulation, and the in vitro invasion capabilities of the stimulated cells were observed using transwell assay. Changes in the proliferation and activity of the JAR cells cultured in vitro following PMA treatment were also observed by MTT assay.
  
  
  RESULTS
  p38 MAPK was activated dose-dependently in JAR cells upon the stimulation by PMA, which significantly enhanced the in vitro invasion of the JAR cells, while treatment of the cells with SB203580, a specific inhibitor of p38 MAPK, inhibited the invasion of the cells. The growth of the cells, as observed from the growth curves, was not affected by the treatment of PMA and/or SB203580.
  
  
  CONCLUSIONS
  Activation of p38 MAPK signal transduction pathway may enhance the invasion capability of JAR cells, and p38 MAPK inhibition may therefore yield new possibility to control the invasion of choriocarcinoma.
authors.affiliation: Department of Obstetrics Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. zhangxq@fimmu.com
authors.forename: Xi-Qian
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Zhao
authors.forename: Zhan-Jun
authors.initials: ZJ
authors.lastname: Pang
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Si-Mei
authors.initials: SM
authors.lastname: Chen
authors.forename: Ren
authors.initials: R
authors.lastname: Luo
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Fu-Qi
authors.initials: FQ
authors.lastname: Xing
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002822:Choriocarcinoma / Q000201:enzymology / Q000473:pathology*; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D009361:Neoplasm Invasiveness; D011247:Pregnancy; D011725:Pyridines / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014594:Uterine Neoplasms / Q000201:enzymology / Q000473:pathology*; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D007093:Imidazoles; D011725:Pyridines; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate; C093642:SB 203580