[Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products].
OBJECTIVE
To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus.


METHODS
The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting.


RESULTS
Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting.


CONCLUSIONS
The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.
Luo
Li-min
LM
Institute of tropic diseases, First Military Medical University, Guangzhou 510515, China.
Li
Ming
M
Xia
Hu
H
Huang
Jian-sheng
JS
Cheng
Bai-hong
BH
Wang
Ping
P
D015153:Blotting, Western; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017325:Hepatitis B Vaccines / Q000627:therapeutic use*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D010957:Plasmids
D000939:Epitopes; D017325:Hepatitis B Vaccines