title: Activation of JNK in rat heart by exercise: effect of training.
abstract: The purpose of the study was to determine whether exercise would activate JNK in the heart and whether chronic exercise training would alter the response. Untrained rats were familiarized with the treadmill and assigned to one of four groups: low intensity (LI), 10 min, 0%, 15 m/min; medium intensity (MI), 10 min, 0%, 33 m/min; high intensity (HI), 10 min, 25%, 33 m/min; long duration (LD), 30 min, 0%, 15 m/min. Another cohort of rats was subjected to a progressive 6 wk high-intensity training protocol that produced a 12% increase in heart mass. In untrained rats, JNK activity was LI: 1.5 (fold nonrun control), MI: 2.0, HI: 2.5, LD: 1.25 immediately after a single bout of exercise. In trained rats, no activation of JNK above baseline was detected after either a 10-min or 1-h bout of exercise. We concluded that treadmill exercise activates JNK in the rat heart in an intensity-dependent manner and that chronic training abrogates the myocardial JNK response to a bout of exercise.
authors.affiliation: Laboratory of Molecular Kinesiology, Division of Kinesiology, 1209 CCRB, 401 Washtenaw Ave., Ann Arbor, MI 48109, USA. boluytm@umich.edu
authors.forename: M O
authors.initials: MO
authors.lastname: Boluyt
authors.forename: A M
authors.initials: AM
authors.lastname: Loyd
authors.forename: M H
authors.initials: MH
authors.lastname: Roth
authors.forename: M J
authors.initials: MJ
authors.lastname: Randall
authors.forename: E Y M
authors.initials: EY
authors.lastname: Song
mesh_terms: D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D048031:JNK Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
chemical_list: D012333:RNA, Messenger; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases