title: Effect of tumor stage and nephrectomy on CD62P expression and sP-selectin concentration in renal cancer.
abstract: The CD62P receptor and its soluble form sP-selectin is a marker of platelet (PLT) activation, and constitutes a ligand for CD24 antigen of neoplastic cells and tumor stroma components. The aim of the study was to evaluate the relationship dynamics of the percentage of CD62P+ platelets, the level of the receptor expression and the concentration of soluble form of sP-selectin in renal cancer. Examinations were performed before and after nephrectomy in patients with renal cancer (group A - 25, T2N0M0; group B - 27, T2N1-2M0) and in control group (C - 24 subjects). The two groups A and B showed an increased subpopulation of CD62P+ platelets (p<0.01) and elevated sP-selectin concentration (p<0.001) before and after nephrectomy. Although following nephrectomy sP-selectin concentration decreased markedly, it was still higher 3 months after the procedure compared to control group (p<0.05). Following nephrectomy, however, no statistically significant differences were found in the % of CD62P+ platelets and the receptor expression. Greater dynamics of changes before and after nephrectomy in the percentage of CD62P+ platelets (B1:B2 p<0.05) and the receptor expression (B1:B3 p<0.001) was observed in patients with local lymph node involvement (group B) while sP-selectin concentration was similar in both groups. Nephrectomy did not normalize intravascular activation of PLT and TNM had no significant effect on the expression of CD62P and concentration of sP-selectin.
authors.affiliation: Department of Laboratory Diagnostics, Medical Academy of Bialystok, Bialystok, 15-274 Poland. mantur@amb.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Mantur
authors.forename: H
authors.initials: H
authors.lastname: Kemona
authors.forename: R
authors.initials: R
authors.lastname: Kozłowski
authors.forename: I
authors.initials: I
authors.lastname: Kemona-Chetnik
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000556:secondary / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation; D010976:Platelet Count
chemical_list: D014408:Biomarkers, Tumor; D019007:P-Selectin