title: [Laser doppler flowmetry--repeatability of the method].
abstract: The aim of the study was to evaluate the repeatability of flow microcirculation measurements by laser doppler flowmetry (Periflix, firm Perimed, Sweden). Measurements of right forearm skin perfusion were performed twice in 3-4 day intervals in 40 healthy subject aged 39.5 +/- 18.3. Two independent investigators analysed the results. Pearson correlation and analysis ac. Bland and Altman (Lancet 1986, 1, 307-310) were performed during comparing results obtained in the same studied persons and by the investigators. There was a high correlation between the compared results performed on the same subjects within a few days interval, especially between the results for post-occlusive congestion reaction in basic temperature (r = 0.64-0.72) and the results for congestion reaction at a temperature of 44 degrees C (r = 0.5-0.56). High correlation values (r = 0.8-0.95) were also observed when comparing both investigators' evaluations. Repeatability evaluation using Bland and Altman analysis was poorer, especially for values observed in resting conditions and for some values observed after stimulation with both investigators (2.5-10% of results were out of interval: mean +/- 2SD). The results show good repeatability of microcirculation flow measurements, especially under stimulation.
authors.affiliation: Katedra i Klinika Chorób Wewnetrznych i Geriatrii CM UJ 31-531 Kraków, ul. Sniadeckich 10. mireknec@poczta.onet.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Grodzicki
authors.forename: Mirosław
authors.initials: M
authors.lastname: Necki
authors.forename: Marcin
authors.initials: M
authors.lastname: Cwynar
authors.forename: Barbara
authors.initials: B
authors.lastname: Gryglewska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001783:Blood Flow Velocity; D001794:Blood Pressure; D005260:Female; D005542:Forearm / Q000098:blood supply; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D011237:Predictive Value of Tests; D012016:Reference Values; D015203:Reproducibility of Results; D012867:Skin / Q000098:blood supply*; D013696:Temperature