title: The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
abstract: The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.
authors.affiliation: Institut für Genomforschung, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany. joern.kalinowski@genetik.uni-bielefeld.de
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Brigitte
authors.initials: B
authors.lastname: Bathe
authors.forename: Daniela
authors.initials: D
authors.lastname: Bartels
authors.forename: Nicole
authors.initials: N
authors.lastname: Bischoff
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
authors.forename: Nicole
authors.initials: N
authors.lastname: Dusch
authors.forename: Lothar
authors.initials: L
authors.lastname: Eggeling
authors.forename: Bernhard J
authors.initials: BJ
authors.lastname: Eikmanns
authors.forename: Lars
authors.initials: L
authors.lastname: Gaigalat
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hartmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Huthmacher
authors.forename: Reinhard
authors.initials: R
authors.lastname: Krämer
authors.forename: Burkhard
authors.initials: B
authors.lastname: Linke
authors.forename: Alice C
authors.initials: AC
authors.lastname: McHardy
authors.forename: Folker
authors.initials: F
authors.lastname: Meyer
authors.forename: Bettina
authors.initials: B
authors.lastname: Möckel
authors.forename: Walter
authors.initials: W
authors.lastname: Pfefferle
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Rey
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.forename: Oliver
authors.initials: O
authors.lastname: Rupp
authors.forename: Hermann
authors.initials: H
authors.lastname: Sahm
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
authors.forename: Iris
authors.initials: I
authors.lastname: Wiegräbe
authors.forename: Andreas
authors.initials: A
authors.lastname: Tauch
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000096:biosynthesis* / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003352:Corynebacterium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014815:Vitamins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000596:Amino Acids; D009097:Multienzyme Complexes; D020543:Proteome; D011994:Recombinant Proteins; D014815:Vitamins; D001224:Aspartic Acid