title: A simplified method for purification of annexin V from human placenta.
abstract: A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.
authors.affiliation: Institute of Biochemistry, NAS of Armenia, Yerevan, Republic of Armenia.
authors.forename: G G
authors.initials: GG
authors.lastname: Poghosyan
authors.forename: V Z
authors.initials: VZ
authors.lastname: Melkonyan
authors.forename: M V
authors.initials: MV
authors.lastname: Mikaelyan
authors.forename: V K
authors.initials: VK
authors.lastname: Gasparyan
mesh_terms: D017304:Annexin A5 / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D017304:Annexin A5