A surface plasmon resonance probe with a novel integrated reference sensor surface.
A surface plasmon resonance (SPR) sensor probe with integrated reference surface is described. In order to fabricate the integrated reference surface, two dielectric layers with different thickness were deposited on the single gold SPR sensor surface via plasma polymerization of hexamethyldisiloxane. The working sensor surface was a 34 nm dielectric layer with immobilized bovine serum albumin (BSA) antigen and an adjacent thin 1 nm dielectric layer without BSA provided reference surface. A specific immunoreaction of anti-BSA antibody was detected after immersion of the SPR probe into sample solution. Simultaneous observation of reference and working surface response enabled determination of the immunoreaction without the need for the baseline measurement. Moreover, compensation of nonspecific adsorption could be confirmed using anti-human serum albumin antibody.
Akimoto
Takuo
T
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-0031, Japan.
Ikebukuro
Kazunori
K
Karube
Isao
I
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D002138:Calibration / Q000592:standards; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007124:Immunoenzyme Techniques / Q000295:instrumentation* / Q000379:methods; D015335:Molecular Probes; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D013499:Surface Properties; D018511:Systems Integration; D014159:Transducers
D000906:Antibodies; D000936:Antigen-Antibody Complex; D020099:Coated Materials, Biocompatible; D015335:Molecular Probes; D012710:Serum Albumin, Bovine