A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells.
A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTC-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4-27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC.
Choong
Meng Ling
ML
Bioprocessing Technology Centre, MD 11 Level 5, 10 Medical Drive, Singapore 117597. btccml@btc.a-star.edu.sg
Tan
Amabel C L
AC
Luo
Biao
B
Lodish
Harvey F
HF
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000166:cytology / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D011388:Prolactin; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; C477730:Prl2c3 protein, mouse; D011388:Prolactin