title: Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings.
abstract: We investigated the ability of synchrotron radiation computed tomography (SR-CT) to demonstrate trabecular microstructure, detail of trabecular surfaces, and mineralization of bones. Eight rat vertebrae, six rat tibiae, and eight minipig vertebrae were scanned using SR-CT at the synchrotron radiation facility Super Photon ring-8GeV (SPring-8). Images obtained using conventional micro-CT, scanning electron microscopy (SEM), and contact microradiography (CMR) were compared with the SR-CT images. SR-CT showed high image quality without visible partial volume effect. Three-dimensional SR-CT revealed shallow concavities in the bone surface, which were considered to correspond to osteoclastic resorption areas, as well as the connectivity, anisotropy, and shape (rod- or platelike) of trabeculae. Two-dimensional SR-CT showed different density along the surface of the trabecular bone, indicating the degree of bone mineralization. In conclusion, SR-CT seems to be a useful tool for delineating trabecular surfaces, evaluating bone mineralization, and revealing precise trabecular structure.
authors.affiliation: Department of Radiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852-8501 Nagasaki, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Ito
authors.forename: Sadakazu
authors.initials: S
authors.lastname: Ejiri
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Jinnai
authors.forename: Jun
authors.initials: J
authors.lastname: Kono
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Akifumi
authors.initials: A
authors.lastname: Nishida
authors.forename: Kentaro
authors.initials: K
authors.lastname: Uesugi
authors.forename: Naoto
authors.initials: N
authors.lastname: Yagi
authors.forename: Mikako
authors.initials: M
authors.lastname: Tanaka
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging*; D002113:Calcification, Physiologic; D005260:Female; D023421:Models, Animal; D051381:Rats; D013131:Spine / Q000000981:diagnostic imaging; D017356:Synchrotrons; D013977:Tibia / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed / Q000379:methods*