title: A simple pen-spotting method for arraying biomolecules on solid substrates.
abstract: We describe a simple, relatively inexpensive method for depositing biomolecules on a solid substrate using Rapidograph drafting pens. The pens can be used without modification to accurately deposit spots between approximately 100 and 600 microm in diameter. When mounted on a suitable microtranslation stage, the pens can be used to easily deposit tens of spots aligned with underlying substrate features such as microfabricated sensors. The pens are particularly convenient because pre-mixed solutions can be stored in the pens for multiple uses. We demonstrate the use of this approach to deposit DNA probes on a microsensor array.
authors.affiliation: Naval Research Laboratory, Washington, DC 20375-5342, USA. paul.sheehan@nrl.navy.mil
authors.forename: P E
authors.initials: PE
authors.lastname: Sheehan
authors.forename: R L
authors.initials: RL
authors.lastname: Edelstein
authors.forename: C R
authors.initials: CR
authors.lastname: Tamanaha
authors.forename: L J
authors.initials: LJ
authors.lastname: Whitman
mesh_terms: D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D015335:Molecular Probes; D004247:DNA