A role for isothiocyanates in plant resistance against the specialist herbivore Pieris rapae.
We experimentally reanalyzed the classic interaction between Pieris rapae, a specialist lepidopteran herbivore, and isothiocyanates (mustard oils) that are characteristic phytochemicals of the Brassicaceae. Previous investigations have suggested that P. rapae is unaffected by isothiocyanates. Using whole plants, root extracts, and a microencapsulated formulation of allyl isothiocyanate, we now show that isothiocyanates reduce herbivore survival and growth, and increase development time, each in a dose-dependent manner. Neither the substrate allyl glucosinolate, nor myrosinase, the enzyme that results in the breakdown of glucosinolates, negatively affected P. rapae. Thus, we present strong evidence for a role for isothiocyanates in plant resistance against the specialist herbivore P. rapae.
Agrawal
Anurag A
AA
Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada. agrawal@botany.utoronto.ca
Kurashige
Nile S
NS
D000222:Adaptation, Physiological; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry* / Q000254:growth & development; D001937:Brassica / Q000737:chemistry* / Q000254:growth & development; D002080:Butterflies / Q000254:growth & development / Q000502:physiology*; D005247:Feeding Behavior; D017879:Isothiocyanates / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011157:Population Dynamics; D016019:Survival Analysis
D017879:Isothiocyanates