title: The influence of an internal electric field upon protein crystallization using the gel-acupuncture method.
abstract: In this work, the influence of an internal electric field upon the crystallization of lysozyme and thaumatin is explored using a modified design of the gel-acupuncture setup. From a crystallographic point of view, the orientation of crystals that grow preferentially over different types of electrodes inside capillary tubes is also evaluated. Finally, the crystal quality and the three-dimensional structure of these proteins grown with and without the electric field influence are analyzed by means of X-ray diffraction methods.
authors.affiliation: Instituto de Química, UNAM Circuito Exterior, C. U. Mexico, D. F. 04510, Mexico.
authors.forename: N
authors.initials: N
authors.lastname: Mirkin
authors.forename: B A
authors.initials: BA
authors.lastname: Frontana-Uribe
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Romero
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Santoyo
authors.forename: A
authors.initials: A
authors.lastname: Moreno
mesh_terms: D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D009113:Muramidase / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D010940:Plant Proteins; D011506:Proteins; C003427:thaumatin protein, plant; D009113:Muramidase