title: Altered expression of angiopoietins during blood-brain barrier breakdown and angiogenesis.
abstract: Angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) belong to a novel family of endothelial growth factors that function as ligands for the endothelial-specific receptor tyrosine kinase, Tie-2. Ang-1 reduces endothelial permeability of noncerebral vessels and has a major role in vascular stabilization and maturation, whereas Ang-2 is thought to be an endogenous antagonist of the action of Ang-1 at Tie-2. Expression of these ligands at the mRNA and protein level were studied during both blood-brain barrier (BBB) breakdown and cerebral angiogenesis occurring in the rat cortical cold-injury model by RT-PCR analysis and immunohistochemistry respectively, during a time course of 6 hours to 6 days. In addition, immunohistochemical detection of fibronectin was used to detect BBB breakdown at the lesion site and dual labeling was used to determine whether the vessels demonstrating BBB breakdown expressed endothelial Ang-1 or Ang-2. Endothelial Ang-1 and Tie-2 proteins were present in all cerebral vessels of normal brain including those of the choroid plexuses, whereas both these proteins as well as Ang-2 were present in choroid plexus epithelium and in ependymal cells, suggesting that angiopoietins have an autocrine effect on these cell types as well. In contrast, in the early phase after injury during the known period of BBB breakdown, increased Ang-2 mRNA and protein and decreased endothelial Ang-1 and Tie-2 proteins were observed. Two to 6 days after injury, the progressive increase in Ang-1 mRNA and protein and the decrease in Ang-2 coincided with cerebrovascular angiogenesis. Confocal microscopy showed colocalization of both Ang-1 and Ang-2 in endothelium of lesion vessels, and our observation of colocalization of Ang-1 and Ang-2 in polymorphonuclear leukocytes and macrophages has not been reported previously. This study demonstrates that Ang-1 is an important factor in maintaining normal homeostasis in the brain. Thus Ang-1 therapy may have therapeutic potential in reducing BBB breakdown and the ensuing edema after massive brain injury.
authors.affiliation: Toronto Western Research Institute, University Health Network, University of Toronto, Toronto, Canada.
authors.forename: Nima
authors.initials: N
authors.lastname: Nourhaghighi
authors.forename: Krystyna
authors.initials: K
authors.lastname: Teichert-Kuliszewska
authors.forename: Jaime
authors.initials: J
authors.lastname: Davis
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Stewart
authors.forename: Sukriti
authors.initials: S
authors.lastname: Nag
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000235:genetics / Q000378:metabolism*; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D002199:Capillary Permeability; D002540:Cerebral Cortex / Q000098:blood supply* / Q000293:injuries / Q000473:pathology; D002831:Choroid Plexus / Q000098:blood supply / Q000473:pathology; D003080:Cold Temperature; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D043925:Angiogenesis Inducing Agents; D042683:Angiopoietin-1; D042702:Angiopoietin-2; C467629:Angpt1 protein, rat; D005353:Fibronectins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger