[Effects of matrine on the relative molecules expression of proliferation and apoptosis in K562 cells].
OBJECTIVE
To study the effects of matrine on proliferation and apoptosis of K562 cells.


METHODS
Cell culture and cell count, cell cycle phases and number of apoptotic cells were analyzed by flow cytometry. Morphology of apoptotic cells was studied by electron microscopy, BCL-6 and proliferating Cell nuclear antigen (PCNA) were checked by Western blot.


RESULTS
Matrine could inhibit the proliferation of K562 cells with inhibition rate of 69% after 72 hours matrine induction; number of K562 cells increased significantly in G1 phase, and number of apoptotic cell was 68% at 5th day of matrine treated cells; the typical morphology of apoptotic cells of K562 cells was observed by electron microscopy; BCL-6 was increased and PCNA was decreased in K562 cells induced by matrine.


CONCLUSIONS
Matrine inhibited the proliferation and induced the apoptosis of K562 cells significantly, the mechanism of which might be related to cell cycle arrest and the change of PCNA and BCL-6.
Zhang
Y Q
YQ
Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Huang
G S
GS
Wang
Z
Z
Guo
Y
Y
Zhang
H Y
HY
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D051560:Proto-Oncogene Proteins c-bcl-6; D011807:Quinolizines; D014157:Transcription Factors / Q000096:biosynthesis; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D011807:Quinolizines; D014157:Transcription Factors; D000093842:Matrines