[Development of a live culture of cold-adapted reassortant influenza vaccines].
Optimal conditions for the cultivation of the MDCK cell lines in the laboratory spinner or by using the Eagle-MEM with or without fetal serum were worked out. The cold-adapted reassortant vaccine strains of virus influenza A/H1N1, A/H3N2 and B are well replicated in the MDCK cells both in a monolayer and in the spinner by using the serum-free medium. A maximum virus titer depends on a multiplicity of infection used in a fetal medium and on the addition of trypsin. Under the optimal conditions, the titer of the studied cold-adapted reassortants, while using a serum-free medium, reaches as much as 10(9.0)-10(9.5) EID50/ml.
Gendon
Iu Z
IuZ
Markushin
S G
SG
Akopova
I I
II
Koptiaeva
N B
NB
Isachenko
A A
AA
Mazurkova
N A
NA
Nechaeva
E A
EA
D000222:Adaptation, Physiological; D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D016895:Culture Media, Serum-Free; D004285:Dogs; D009980:Influenza A virus / Q000276:immunology / Q000502:physiology*; D009981:Influenza B virus / Q000276:immunology / Q000502:physiology*; D007252:Influenza Vaccines / Q000276:immunology*; D016865:Reassortant Viruses / Q000276:immunology / Q000502:physiology*; D014779:Virus Replication
D016895:Culture Media, Serum-Free; D007252:Influenza Vaccines