title: Growth hormone enhances proinflammatory cytokine production by monocytes in whole blood.
abstract: Growth hormone (GH) has been used as anabolic therapy to treat catabolic patients. In a recent study, however, administration of high doses of GH to critically ill adults was associated with an increase in morbidity and mortality. Preponderance of septic shock and uncontrolled infections as causes of death in these patients suggests an immuno-modulatory effect of GH. Our hypothesis was that GH treatment may modulate the production of proinflammatory cytokines, which are implicated in sepsis. In our study, human monocytes in whole blood were activated with lipopolysaccaharide (LPS) (1-100 ng/ml) purified from a clinical isolate of group B Neisseria meningitidis in the presence of a high dose of GH (100 ng/ml). The subsequent proinflammatory cytokine response was analysed by intracellular cytokine staining and flow cytometry. Our results show that GH enhances IL1-alpha, IL-6 and TNF-alpha production by LPS activated monocytes in whole blood. The modulation of cytokines by GH may be responsible for the adverse consequences of GH in critically ill patients.
authors.affiliation: Institute of Child Health, Immunobiology, 30 Guilford Street, London WC1N 1EH, UK. h.uronen@ich.ucl.ac.uk
authors.forename: H
authors.initials: H
authors.lastname: Uronen-Hansson
authors.forename: M L
authors.initials: ML
authors.lastname: Allen
authors.forename: E
authors.initials: E
authors.lastname: Lichtarowicz-Krynska
authors.forename: A
authors.initials: A
authors.lastname: Aynsley-Green
authors.forename: T J
authors.initials: TJ
authors.lastname: Cole
authors.forename: I
authors.initials: I
authors.lastname: Höidén-Guthenberg
authors.forename: L
authors.initials: L
authors.lastname: Fryklund
authors.forename: N
authors.initials: N
authors.lastname: Klein
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000187:drug effects / Q000276:immunology; D020126:Brefeldin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000097:blood / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D020126:Brefeldin A; D013006:Growth Hormone