Application of solid-phase extraction for determination of phenolic compounds in barrique wines.
A fast, selective and sensitive chromatographic method has been developed for determination of gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, benzoic, ferulic, sinapic, cinnamic, and ellagic acids and p-hydroxybenzaldehyde, vanillin, syringaldehyde, 2-furfural, 5-methylfurfural, and 5-methoxyfurfural. The compounds from untreated wine samples were pre-concentrated and cleaned using solid-phase extraction on RP-105 polymeric sorbent. The cartridge was conditioned with methanol and water. Co-extracted ballast substances were rinsed from the sorbent with 0.1 mol L(-1) hydrochloric acid-methanol, 1:4 (v/ v). Retained phenolic compounds were selectively eluted with diethyl ether. A linear mobile phase gradient containing 0.3% acetic acid and methanol was used for final baseline chromatographic separation on a Hypersil BDS C18 column. Limits of detection (LOD=3 s(bl)) in the range 5.2 to 181.2 microg L(-1), resolution (R) better than 1.7, and repeatability of 2.7-5.1% (RSD for real samples) were achieved. The method was applied for quantification of individual phenolic compounds in barrique wines.
Matejícek
D
D
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelská 1, 613 00 Brno, Czech Republic.
Klejdus
B
B
Mikes
O
O
Sterbová
D
D
Kubán
V
V
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014920:Wine / Q000032:analysis*
D010636:Phenols