Pyrene as a fluorescent probe for DNA base radicals.
The steady-state emission spectra of 5-(1-pyrenyl)-modified pyrimidine and 8-(1-pyrenyl)-modified purine nucleosides in water at different pH values provide important information about the acidity or basicity of photochemically generated DNA base radicals which are key intermediates in DNA-mediated charge transport processes.
Huber
Robert
R
Technical University of Munich, Institute for Organic Chemistry and Biochemistry, D-85747 Garching, Germany.
Fiebig
Torsten
T
Wagenknecht
Hans-Achim
HA
D004247:DNA / Q000737:chemistry*; D004583:Electrons; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes; D011687:Purines / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D005609:Free Radicals; D015335:Molecular Probes; D011687:Purines; D011721:Pyrenes; D011743:Pyrimidines; D014867:Water; D004247:DNA