title: IS1999 increases expression of the extended-spectrum beta-lactamase VEB-1 in Pseudomonas aeruginosa.
abstract: The integron-borne bla(VEB-1) gene encodes an extended-spectrum beta-lactamase. This gene was associated mostly with IS1999 and rarely with an additional IS2000 element in Pseudomonas aeruginosa isolates from Thailand, whereas IS1999 was only very rarely associated with bla(VEB-1) in Enterobacteriaceae. Expression experiments and promoter study identified promoter sequences in IS1999 that increased the expression of VEB-1 in P. aeruginosa.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Aubert
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C119424:beta-lactamase VEB-1, E coli; D001618:beta-Lactamases