Expression of cytokines, neurotrophins, neurotrophin receptors and NOS mRNA in dorsal root ganglion of a rat tourniquet model.
We studied temporal changes in mRNA expression patterns for nitric oxide synthase (NOS), cytokines, neurotrophins and neurotrophin receptors in the dorsal root ganglion (DRG) of the rat, after application of a tourniquet to the hind limb. Collapsed myelin and degenerated axons were observed in the tourniquet segment of the sciatic nerve. Gene expression level of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS) was significantly increased in ipsilateral DRG samples at 4h after application of the tourniquet but not in the contralateral or control DRG samples. Upregulation of tumor necrosis factor (TNF)-alpha, activating transcription factor (ATF)-3 and neurotrophin-3 (NT3) expressions began at 1h after application of the tourniquet in ipsilateral DRGs. It is likely that transient expression of these molecules triggers secondary events that may be beneficial to wound repair and regeneration.
Mizusawa
Ikubumi
I
Department of Legal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. mizusawa@fmu.ac.jp
Abe
Sumiko
S
Kanno
Katsuyuki
K
Yabashi
Atsuko
A
Honda
Takashi
T
Suto
Miwako
M
Hiraiwa
Kouichi
K
D051700:Activating Transcription Factor 3; D000818:Animals; D001369:Axons / Q000473:pathology; D005727:Ganglia, Spinal / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D009186:Myelin Sheath / Q000473:pathology; D020933:Neurotrophin 3 / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries*; D014157:Transcription Factors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D020933:Neurotrophin 3; D012333:RNA, Messenger; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D019001:Nitric Oxide Synthase