[Expression of mitogen-activated protein kinase in vascular tissues after coronary artery balloon injury in rat].
OBJECTIVE
To observe the changes in mitogen-activated protein kinase (MAPK) activity and gene expression after coronary artery balloon injury in rat.


METHODS
Forty Wistar rats were randomly divided into control group (without coronary artery balloon injury), and 3, 7 and 14 days after coronary artery balloon injury groups (n=10, respectively). The activity of MAPK was measured by biochemical method and the gene expression was examined by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
MAPK activity and gene expression of MAPK in the coronary artery balloon injury in rat after 3, 7 and 14 days were significantly higher than that of normal control, especially 3 days after injury [MAPK activity: (17.32+/-2.17) pmol x mg(-1) x min(-1);MAPK protein: ERK1 was 194.7+/-8.6, ERK2 was 175.8+/-7.9; MAPK gene expression: ERK1 was 1.15+/-0.21, ERK2 was 1.13+/-0.14 ].


CONCLUSIONS
The increase activity and mRNA of MAPK may be involved in the restenosis process after coronary balloon injury.
Ge
Hua
H
Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China.
Zeng
Ding-Yin
DY
Zhao
Wei-hua
WH
Qi
Guo-xian
GX
D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects*; D000818:Animals; D023903:Coronary Restenosis; D003331:Coronary Vessels / Q000201:enzymology*; D015870:Gene Expression; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3