Bradykinins and their cDNA from piebald odorous frog, Odorrana schmackeri, skin.
Bradykinin, (des-Arg(9))-bradykinin and bradykinyl-VAPAS, were identified in the skin secretion of the piebald odorous frog, Odorrana schmackeri. Using 3'- and 5'-RACE reactions, bradykinin precursor cDNA was cloned and found to contain an open-reading frame of 311 amino acid residues. The preprobradykinin was found to consist of a putative signal peptide of approximately 20 amino acid residues, followed by seven tandem repeat coding domains of 43-44 amino acids. Bradykinin and its C-terminal extended molecular form were encoded on this single precursor and could be generated by differential post-translational processing.
Li
Long
L
Pharmaceutical Biotechnology Research Group, School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK.
Bjourson
Anthony J
AJ
He
Jianyuan
J
Cai
Guangxian
G
Rao
Pingfan
P
Shaw
Chris
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001920:Bradykinin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D011898:Ranidae / Q000235:genetics*; D012867:Skin / Q000737:chemistry / Q000378:metabolism*
D018076:DNA, Complementary; D011498:Protein Precursors; D001920:Bradykinin