Fibroblast cell shape and adhesion in vitro is altered by overexpression of the 7a and 7b isoforms of protocadherin 7, but not the 7c isoform.
Protocadherins (Pcdhs) are a family of cadherins considered to play an important role in the cell-cell adhesion of specific neurons in the central nervous system. Of the reported Pcdhs, relatively little is known about the functional role of protocadherin 7 (Pcdh7), and there is no evidence of Pcdh7 mediated cell-cell adhesion. To date, three splicing variants are known; they may have different effects on cell phenotype. We report here that mouse fibroblast L cells stably overexpressing the Pcdh7 isoforms 7a and 7b, but not 7c, showed a morphological change and Ca(2+)dependent cell adhesion.
Yoshida
Kenichi
K
Genetic Diagnosis, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. yoshidak@ims.u-tokyo.ac.jp
D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D048429:Cell Size; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D007739:L Cells; D051379:Mice; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
C121291:CDH7 protein, human; D015820:Cadherins; D020033:Protein Isoforms