title: Protein PknE, a novel transmembrane eukaryotic-like serine/threonine kinase from Mycobacterium tuberculosis.
abstract: Protein PknE from Mycobacterium tuberculosis has been overproduced and purified, and its biochemical properties have been analyzed. This protein is shown to be a eukaryotic-like (Hanks'-type) protein kinase with a structural organization similar to that of membrane-bound eukaryotic sensor serine/threonine kinases. It consists of a N-terminal catalytic domain located in the cytoplasm, linked via a single transmembrane-spanning region to an extracellular C-terminal domain. The full-length enzyme, as well as the cytosolic domain alone, can autophosphorylate on serine and threonine residues. Such autokinase activity requires the presence of a lysine residue at position 45 in subdomain II, which is known to be essential also for eukaryotic kinase activity. Involvement of PknE in the transduction of external signals into the cytosol of bacteria is proposed.
authors.affiliation: Institut de Biologie et Chimie des Protéines, Université de Lyon, Centre National de la Recherche Scientifique, Lyon, France. virginie_molle@yahoo.fr
authors.forename: Virginie
authors.initials: V
authors.lastname: Molle
authors.forename: Christine
authors.initials: C
authors.lastname: Girard-Blanc
authors.forename: Laurent
authors.initials: L
authors.lastname: Kremer
authors.forename: Patricia
authors.initials: P
authors.lastname: Doublet
authors.forename: Alain J
authors.initials: AJ
authors.lastname: Cozzone
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Prost
mesh_terms: D020816:Amino Acid Motifs; D020134:Catalytic Domain; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008239:Lysine / Q000737:chemistry; D008957:Models, Genetic; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013912:Threonine / Q000737:chemistry
chemical_list: D011993:Recombinant Fusion Proteins; D013912:Threonine; D012694:Serine; D005982:Glutathione Transferase; D011494:Protein Kinases; C477875:PknE protein, Mycobacterium tuberculosis; D017346:Protein Serine-Threonine Kinases; D008239:Lysine