Lipid peroxidation and antioxidant defence status during larval development and metamorphosis of giant prawn, Macrobrachium rosenbergii.
In the present communication we studied the involvement of reactive oxygen species and alteration in antioxidant defence status during larval development and metamorphosis of giant prawn, Macrobrachium rosenbergii. Overall results indicate that there was a decline in endogenous lipid peroxidation level during larval development. Activity of superoxide dismutase was the lowest in early larval stages (Zoea-I and II) and thereafter increased in V and VI stages, followed by a decrease in the subsequent larval stages. Catalase and glutathione peroxidase did not exhibit specific pattern of changes during development. Reduced glutathione content exhibited an incremental increase during larval progression until metamorphosis. Ascorbic acid content of the larval tissue remained unaltered during development but a sharp fall was marked in its content in the post-larvae. Hence it is concluded that early larvae face high oxidative stress as evident from the high content of thiobarbituric acid reactive substances. This may be due to direct exposure of larvae to ambient oxygen of the water as well as their low antioxidant potential. However, during development with the augmentation in antioxidant reserve of the larval tissues a diminution in the oxidative stress was recorded. Thus it is presumed that antioxidant defences play an important role in providing protection to the developing larvae from oxidative assault during larval progression and metamorphosis.
Dandapat
Jagneshwar
J
Department of Biotechnology and Department of Zoology, Utkal University, Bhubaneswar 751004, India.
Chainy
Gagan B N
GB
Rao
K Janardhana
KJ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008675:Metamorphosis, Biological / Q000502:physiology*; D033541:Palaemonidae / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D005978:Glutathione