Cryptosporidium parvum Cpn60 targets a relict organelle.
Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.
Riordan
Christina E
CE
Wadsworth Center, New York State Department of Health, Axelrod Institute for Public Health, 120 New Scotland Avenue, Albany, NY 12208, USA.
Ault
Jeffrey G
JG
Langreth
Susan G
SG
Keithly
Janet S
JS
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D016000:Cluster Analysis; D016785:Cryptosporidium parvum / Q000235:genetics*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D015723:Gene Library; D049452:Green Fluorescent Proteins; D007106:Immune Sera; D007150:Immunohistochemistry; D016013:Likelihood Functions; D008164:Luminescent Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000235:genetics* / Q000648:ultrastructure; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D034101:Sporozoites / Q000648:ultrastructure*
D018834:Chaperonin 60; D017931:DNA Primers; D007106:Immune Sera; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins