Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines.
While alpha- and beta-synuclein largely overlap in their expression in the vertebrate brain, only alpha-synuclein accumulates in the fibrillar aggregates typical of Parkinson's disease. It is thus critical to have immunological reagents that distinguish between these two protein isoforms. The monoclonal antibody Syn-1 (Transduction Labs) has been frequently used for the specific detection of alpha-synuclein. In this report, the epitope for Syn-1 is localized within residues 91-99 of human alpha-synuclein. Sequence differences exist in this domain that account for the specificity of Syn-1 for alpha- versus beta-synuclein. However, Syn-1 also displays reactivity with additional species (approximately 45 kDa) in brain homogenates from both wild-type and alpha-synuclein null mice, indicating a potential for cross-reactivity with a protein species that is unrelated to alpha-synuclein in brain tissue or extracts.
Perrin
R J
RJ
Medical Scholars Program, University of Illinois, Urbana, IL 61801, USA.
Payton
J E
JE
Barnett
D H
DH
Wraight
C L
CL
Woods
W S
WS
Ye
L
L
George
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001921:Brain / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D018604:Epitope Mapping; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D051381:Rats; D011994:Recombinant Proteins; D013045:Species Specificity; D051843:Synucleins; D051844:alpha-Synuclein; D051846:beta-Synuclein
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C497604:SNCA protein, human; C497607:SNCB protein, human; C497605:Snca protein, mouse; C497606:Snca protein, rat; C497608:Sncb protein, mouse; C497609:Sncb protein, rat; D051843:Synucleins; D051844:alpha-Synuclein; D051846:beta-Synuclein