Sub-femtomole peptide detection in ion mobility-time-of-flight mass spectrometry measurements.
Sensitivity and detection limit enhancements are obtained for peptides by performing high repetition rate (150 Hz) matrix-assisted laser desorption/ionization (MALDI) coupled with ion mobility-time-of-flight mass spectrometry. Absolute limits of detection (3sigma) for model peptides are on the order of 0.1 fmol of peptide deposited and represent a factor of 40-60 improvement over data obtained using typical low repetition (20 Hz) MALDI. This increase in sensitivity is demonstrated for two-dimensional MALDI-IM-TOFMS peptide mass mapping of bovine hemoglobin.
McLean
John A
JA
Laboratory for Biological Mass Spectrometry, Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Russell
David H
DH
D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D006454:Hemoglobins; D010446:Peptide Fragments; D000804:Angiotensin II; D000803:Angiotensin I