Role for calcium in heat shock-mediated synaptic thermoprotection in Drosophila larvae.
Chemical synaptic transmission is the mechanism for fast, excitation-coupled information transfer between neurons. Previous work in larval Drosophila has shown that transmission at synaptic boutons is protected by heat shock exposure from subsequent thermal stress through pre- and postsynaptic modifications. This protective effect has been, at least partially, ascribed to an up-regulation in the inducible heat shock protein, hsp70. Effects of hsp70 are correlated with changes to intracellular calcium handling, and the dynamics of intracellular calcium regulate synaptic transmission. Consistent with such a relationship, synaptic plasticity increases at locust neuromuscular junctions following heat shock, suggesting an effect of heat shock on residual presynaptic calcium. Intracellular recording from single abdominal muscle fibers of Drosophila larvae showed that prior heat shock imparts thermoprotection by increasing the upper temperature limit for synaptic transmission. Heat shock exposure enhances short-term synaptic plasticity and increases its thermosensitivity. Increasing extracellular calcium levels eliminates the physiological differences between control and heat shock preparations; excess calcium itself induces thermoprotection at elevated concentrations. These data support the hypothesis that stress-induced neuroprotection at the nerve terminal acts, at least partially, through an alteration to the physiological effects of residual presynaptic calcium.
Barclay
J W
JW
Department of Biology, Queen's University, Kingston, ON, Canada K7L 3N6.
Robertson
R M
RM
D000818:Animals; D002118:Calcium / Q000502:physiology*; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D006358:Hot Temperature; D007814:Larva; D009473:Neuronal Plasticity / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology; D013569:Synapses / Q000502:physiology*
D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D002118:Calcium