title: Microsatellite instability and MBD4 mutation in unselected colorectal cancer.
abstract: BACKGROUND
  We investigated the prognostic significance of microsatellite instability (MSI) and the association with clinicopathological factors in colorectal cancer, and further identified MBD4 mutations and their clinicopathological significance.
  
  
  METHODS
  MSI was analyzed in 201 colorectal cancers. Sequencing analysis of MBD4 was performed in 26 MSI and 28 microsatellite stable (MSS) tumors.
  
  
  RESULTS
  Twenty-seven tumors (13.4%) were MSI but did not correlate with improved survival. MSI was significantly correlated with proximal colon tumors (p < 0.001), poor differentiation or mucinous type (p = 0.005) and multiple tumors (p = 0.04). MBD4 mutations were found in 15% MSI but not in MSS tumors. The mutated cases showed female overrepresentation, proximal site and poorly-differentiated/mucinous type.
  
  
  CONCLUSIONS
  MSI was not correlated with survival, but shared other features associated with MSI in colorectal cancer as demonstrated by others. The clinicopathological variables associated with the MBD4 mutations were probably the reflection of MSI features.
authors.affiliation: Division of Oncology, Department of Biomedicine and Surgery, Linköping University, S-581 85 Linköping, Sweden. sofev@ibk.liu.se
authors.forename: Sofia
authors.initials: S
authors.lastname: Evertson
authors.forename: Asa
authors.initials: A
authors.lastname: Wallin
authors.forename: Gunnar
authors.initials: G
authors.lastname: Arbman
authors.forename: Sabine
authors.initials: S
authors.lastname: Rütten
authors.forename: Anna
authors.initials: A
authors.lastname: Emterling
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Sun
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004706:Endodeoxyribonucleases / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation
chemical_list: D004706:Endodeoxyribonucleases; C118712:MBD4 protein, human