Partial purification and characterisation of a 2,4,5-trichlorophenol detoxifying O-glucosyltransferase from wheat.
An enzyme preparation with UDP-glucose-dependent O-glucosyltransferase (OGT; EC 2.4.1.-) activity toward 2,4,5-trichlorophenol has been purified 215-fold from wheat shoots. The OGT co-purified with the major extractable glucosylating activity toward the flavonol quercetin and was characterised as a monomeric 53 kDa protein. Among the xenobiotic phenols tested, the purified enzyme preparation showed at least a 10-fold preference for 2,4,5-trichlorophenol. When assayed with flavonoids, the OGT was active toward flavonols and coumestrol, showing a clear preference for 3-hydroxy flavone when incubated with a range of monohydroxylated flavonoids. It was concluded that the major 2,4,5-trichlorophenol-detoxifying OGT in wheat shoots is most probably a flavonol-3-O-glucosytransferase.
Brazier
Melissa
M
Crop Protection Group, University of Durham, Durham DH1 3LE, UK.
Cole
David J
DJ
Edwards
Robert
R
D002250:Carbon Radioisotopes; D002733:Chlorophenols / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006031:Glycosylation; D008658:Inactivation, Metabolic; D008970:Molecular Weight; D018520:Plant Shoots / Q000737:chemistry; D013379:Substrate Specificity; D014908:Triticum / Q000201:enzymology*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism
D002250:Carbon Radioisotopes; D002733:Chlorophenols; D005419:Flavonoids; D015262:Xenobiotics; C009534:2,4,5-trichlorophenol; D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose