title: DNA capillary electrophoresis using poly(vinyl alcohol). I. Inner capillary coating.
abstract: Two new methods of inner capillary coating with poly(vinyl alcohol) (PVAL) have been investigated and evaluated by performing DNA capillary electrophoresis (CE) using PVAL as a separation medium and by measuring the electroosmotic flow (EOF) mobility. The treatment of capillaries with a silanol-group modified PVAL (PVAL-Si) has been found to give good coating effects for improving the resolution of DNA CE and for reducing the EOF. This coating must be effectively achieved by combining the adsorptive property of PVAL chains onto silica with the reaction between the silanol groups of PVAL-Si and the silica surface. The adsorption of PVAL onto silica has been observed by using atomic force microscopy (AFM) for PVAL-Si as well as for a nonmodified PVAL as a control. The coating with PVAL that links to the capillary wall surface with more hydrolytically stable bonding, -Si-C-, has been formed by performing the Grignard reaction, followed by in-capillary polymerization of vinyl acetate (VAc) and hydrolysis. This coating has been found to be effective for improving the resolution of DNA CE and for reducing the EOF.
authors.affiliation: Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
authors.forename: Tohei
authors.initials: T
authors.lastname: Moritani
authors.forename: Kyunghwan
authors.initials: K
authors.lastname: Yoon
authors.forename: Miriam
authors.initials: M
authors.lastname: Rafailovich
authors.forename: Benjamin
authors.initials: B
authors.lastname: Chu
mesh_terms: D000327:Adsorption; D004247:DNA / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D018625:Microscopy, Atomic Force; D011142:Polyvinyl Alcohol; D012822:Silicon Dioxide; D013499:Surface Properties
chemical_list: D012822:Silicon Dioxide; D011142:Polyvinyl Alcohol; D004247:DNA