[Down-regulated full-length novel gene GDDR and its effect on gastric cancer].
OBJECTIVE
To clone gene fragments from suppression substructive library established for screening down-regulated genes in gastric carcinoma, and obtain the full-length novel gene.


METHODS
Gene fragments were identified by sequencing plasmids of positive colonies chosen randomly. One gene fragment was amplified by RACE, and the full-length novel gene was obtained. Expression of novel gene mRNA was respectively detected by semi-quantitative PCR in the gastric carcinoma tissues and counterpart normal gastric mucous membrane of 25 patients with gastric cancer. The structure of the full-length novel gene, location on chromosome, property of protein encoded by full-length novel gene and its function were investigated by Bio-message technique.


RESULTS
One 331 bp gene fragment was cloned, and its full-length novel gene obtained by RACE. The novel full-length gene was named GDDR, registered in the number of AF494509 by GenBank. Significant down-regulated expression of GDDR gene mRNA in gastric carcinoma tissues was confirmed (GDDR/beta-actin 13.474 +/- 5.059 vs 1.041 +/- 0.202, P < 0.01). GDDR was located in chromosome 2p13 with 5 exons. As one member of new BRICHO family as CA11, GDDR encoding protein with transmembrane peptide revealed homology to protein encoded by CA11.


CONCLUSIONS
A novel full-length gene GDDR is obtained. GDDR likely is another gene of BRICHO family related to gastric cancer.
Du
Jian-jun
JJ
Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xian 710032, China.
Dou
Ke-feng
KF
Peng
Shu-you
SY
Wang
Wei-zhong
WZ
Wang
Zhong-hua
ZH
Xiao
Hua-sheng
HS
Guan
Wen-xian
WX
Liu
Ying-bin
YB
Gao
Zhi-qing
ZQ
D000595:Amino Acid Sequence; D002352:Carrier Proteins; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000235:genetics*
D002352:Carrier Proteins; D018076:DNA, Complementary; C503073:GKN2 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger