The production of lipids alternately labelled with carbon-13.
Chlorella cells were shown to have similar fatty acid profiles when grown photoautotrophically or if grown photoheterotrophically with ethanoate (acetate) as carbon source. When supplied with ethanoate labelled with carbon-13 in the methyl group, the alga incorporated it into fatty acids with retention of the sequence of labelling on alternate carbon atoms, thus providing a convenient method for synthesising lipids in a form useful for nuclear magnetic resonance (NMR) studies of lipids in situ in membranes. Marine algae used in fish farming may have higher levels of very highly unsaturated fatty acids; proposals for producing these compounds labelled with carbon-13 are, therefore, presented, based on using centrally labelled glycerol. The scope for producing other substances labelled in a form suitable for NMR studies, such as carotenoids, is discussed.
Boyle-Roden
Elizabeth
E
Department of Nutrition and Food Science, College of Agriculture, University of Maryland at College Park, 3304 Marie Mount Hall, College Park, MD 20742-7521, USA.
German
J B
JB
Wood
B J B
BJ
D002247:Carbon Isotopes / Q000737:chemistry* / Q000378:metabolism*; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry* / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy
D002247:Carbon Isotopes; D005231:Fatty Acids, Unsaturated; D008055:Lipids