title: [Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products].
abstract: OBJECTIVE
  To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus.
  
  
  METHODS
  The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting.
  
  
  RESULTS
  Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting.
  
  
  CONCLUSIONS
  The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.
authors.affiliation: Institute of tropic diseases, First Military Medical University, Guangzhou 510515, China.
authors.forename: Li-min
authors.initials: LM
authors.lastname: Luo
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Hu
authors.initials: H
authors.lastname: Xia
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Huang
authors.forename: Bai-hong
authors.initials: BH
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017325:Hepatitis B Vaccines / Q000627:therapeutic use*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D010957:Plasmids
chemical_list: D000939:Epitopes; D017325:Hepatitis B Vaccines