[Application of a micromethod in ovarian cancer chemosensitivity testing in vitro].
OBJECTIVE
To assess the efficacy of microdetermination in the ATP-bioluminescence assay (ATP-CVA) of ovarian cancer chemosensitivity testing in vitro.


METHODS
A micromethod ATP-CVA was employed in determining the chemosensitivity of 35 specimens of fresh ovarian cancer cells using Victor 2 Multilabel Counter.


RESULTS
1. By use of Victor 2 Multilabel Counter to determine ATP, in a range of 10(-9)-10(-5) mol/ml, a linear relationship was remarkable between the log ATP and the log luminescence. The equation was Y = 0.892X + 10.257, the correlation coefficient r was 0.998, P < 0.01. 2. The coefficient of variation obtained from ATP-CVA was 7.3%. 3. The sensitivity of the test was 92.0%, the specificity 70.0%, and the total predictive accuracy of ATP-CVA 87.1%.


CONCLUSIONS
With the use of Victor 2 Multilabel Counter, ATP-CVA is fast, cost-effective, and easy to perform with only small error.
Liu
Shanling
S
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Qian
Xiaolei
X
Peng
Zhilan
Z
Wang
He
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D000255:Adenosine Triphosphate