title: [Interfering effects of radix Salviae miltiorrhizae and lingustrazine on mm-LDL activating BKCa in ECV304 cell].
abstract: OBJECTIVE
  To observe the action of minimally modified low density lipoprotein (mm-LDL) on BKCa in ECV304 cell and the interfering effects of radix salviae miltiorrhizae extract powder 764-3 (30 micrograms/ml) and lingustrazine (200 micrograms/ml) on this action.
  
  
  METHODS
  The cell-attached configuration of patch clamp technique was applied.
  
  
  RESULTS
  mm-LDL (100 micrograms/ml) potentiated the activity of BKCa in ECV304. While 764-3 and lingustrazine abolished it.
  
  
  CONCLUSIONS
  mm-LDL acted on vascular endothelial cell ECV304 could rapidly activate the activity of BKCa and might result in the increase of electro-chemical gradient for the resting Ca2+ influx, thus resting cytoplasmic concentration of calcium could be elevated and endothelial dysfunction would be induced. 764-3 and lingustrazine might have the protective action through decreasing the activity of BKCa.
authors.affiliation: Department of Physiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
authors.forename: J H
authors.initials: JH
authors.lastname: Qu
authors.forename: G H
authors.initials: GH
authors.lastname: Bao
mesh_terms: D002478:Cells, Cultured; D004336:Drug Antagonism; D006801:Humans; D030004:Ligusticum; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology*; D024681:Potassium Channels, Calcium-Activated / Q000187:drug effects*; D027561:Salvia miltiorrhiza; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D008077:Lipoproteins, LDL; D028321:Plant Preparations; D024681:Potassium Channels, Calcium-Activated; C099252:oxidized low density lipoprotein