title: Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method.
abstract: Biosynthesis of (S)-(+)-2-amino-4-phenylbutanoic acid (1) was performed by nonenantioselective hydantoinase and L-N-carbamoylase using racemic 5-[2-phenylethyl]-imidazolidine-2,4-dione (rac-2) as a substrate. The compounds involved in this biocatalysis process could be simultaneously resolved by high-performance liquid chromatography using Chirobiotic T column with a mobile phase of EtOH/H(2)O = 10/90 at pH 4.2-4.5. To our knowledge, this is the first report of the successful production of 1 by the combination of recombinant hydantoinase and L-N-carbamoylase.
authors.affiliation: Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Hsueh-Hsia
authors.initials: HH
authors.lastname: Lo
authors.forename: Chao-Hung
authors.initials: CH
authors.lastname: Kao
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Lee
authors.forename: Teng-Kuei
authors.initials: TK
authors.lastname: Yang
authors.forename: Wen-Hwei
authors.initials: WH
authors.lastname: Hsu
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry*; D000613:Aminobutyrates / Q000138:chemical synthesis*; D001407:Bacillus / Q000201:enzymology; D002384:Catalysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D000613:Aminobutyrates; D011994:Recombinant Proteins; D000581:Amidohydrolases; C020047:dihydropyrimidinase; C479550:2-amino-4-phenylbutanoic acid