Immunohistochemical study on the induction of heme oxygenase-1 by traumatic brain injury.
We investigated the dynamics of the induction of heme oxygenase-1 (HO-1) in the human brain after death caused by traumatic brain injury (TBI). HO-1 was found to stain neurons and microglia/macrophages in cases with TBI, whereas no positive staining except for a few round cells in the arachnoidal space was observed in control cases. In a case with 7h survival, a considerable number of HO-1 positive neurons and microglia were observed. The number of HO-1 positive cells and level of HO-1 staining gradually increased up to 24h survival. Although HO-1 positive neurons were seldom observed in cases with more than 7-day survival, HO-1 positive microglia were still observed even in cases with 5-month survival. The results indicate that HO-1 may be induced by TBI in human cases, and suggest that prolonged HO-1 induction in microglia might reflect its role in protecting those cells from secondary damage including oxidative stress.
Orihara
Yoshiyuki
Y
Department of Legal Medicine, Faculty of Medicine, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima City 890-8520, Japan. orihara@m3.kufm.kagoshima-u.ac.jp
Tsuda
Ryouichi
R
Ikematsu
Kazuya
K
Nakasono
Ichiro
I
Ogata
Mamoru
M
D001930:Brain Injuries / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000201:enzymology*; D008565:Membrane Proteins; D017628:Microglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D013997:Time Factors
D008565:Membrane Proteins; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1