title: Rapid subpicogram protein detection on a microchip without denaturing.
abstract: Protein size separation based on sodium dodecyl sulfate-gel electrophoresis (SDS-GE) requires denaturing, but we propose that denaturing is unnecessary for analysis by microchip electrophoresis (micro-CE). By omitting the protein denaturing process, we achieved not only shortened total analysis time, but also dramatically improved sensitivity without compromising size determination. The detection limit was improved to 0.1 ng/microL under conditions without denaturing and 600 pg (9.0 femtomol) of bovine serum albumin was detectable, which equals levels detectable by Silver stain, although a routine method by microchips in the Coomassie Blue detection level.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Tokushima, Japan. tabuchi@ph.tokushima-u.ac.jp
authors.forename: Mari
authors.initials: M
authors.lastname: Tabuchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D030562:Databases, Protein; D004586:Electrophoresis / Q000379:methods; D006360:Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D019169:Jurkat Cells / Q000737:chemistry; D013058:Mass Spectrometry; D008832:Microchemistry / Q000379:methods*; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D006360:Heat-Shock Proteins; D011506:Proteins; D020543:Proteome