[D-limonene induces apoptosis of gastric cancer cells].
OBJECTIVE
To investigate the apoptosis effect induced by D-limonene on BGC-823 gastric cancer cells.


METHODS
The expression of p53, bc1-2 in BGC-823 cells and qualitative, quantitative index of cell apoptosis were detected with MTT, electron microscopy, flow cytometry and immunohistochemical method.


RESULTS
D-limonene could induce the formation of apoptotic bodies in a dose- and time-dependent manner. The expression of bcl-2 protein decreased and p53 protein increased in BGC-823 cells treated with D-limonene, compared with the control cells.


CONCLUSIONS
D-limonene exerts its cytotoxic effect on BGC-823 gastric cancer cells by inducing apoptosis.
Lu
Xiao-guang
XG
Department of General Surgery, Fourth Hospital of Dalian Medical University, Dalian 116001, China.
Feng
Bing-an
BA
Zhan
Li-bin
LB
Yu
Zhi-hong
ZH
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D053138:Cyclohexenes; D004305:Dose-Response Relationship, Drug; D006801:Humans; D000077222:Limonene; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D053138:Cyclohexenes; D019253:Proto-Oncogene Proteins c-bcl-2; D013729:Terpenes; D016159:Tumor Suppressor Protein p53; D000077222:Limonene