title: Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells.
abstract: Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After proliferation of undifferentiated ES-like cells in vitro, some bovine ES-like cells differentiated to neural precursor cells, which were cultured in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet-derived growth factor (PDGF). In addition, calves were successfully cloned using ES-like cells and the frequency of term pregnancies for blastocysts derived from ES-like cells was higher than those of early pregnancies and maintained pregnancies after nuclear transplantation (NT) with bovine somatic cells. Successful cloning from bovine ES-like cells should allow the introduction into cattle of specific genetic characteristics of biomedical and/or agricultural importance.
authors.affiliation: Saito Laboratory of Cell Technology, Yaita, Tochigi, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Saito
authors.forename: Ken
authors.initials: K
authors.lastname: Sawai
authors.forename: Hideyo
authors.initials: H
authors.lastname: Ugai
authors.forename: Satoru
authors.initials: S
authors.lastname: Moriyasu
authors.forename: Akira
authors.initials: A
authors.lastname: Minamihashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hirayama
authors.forename: Soichi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Pan
authors.forename: Takehide
authors.initials: T
authors.lastname: Murata
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Obata
authors.forename: Kazunari K
authors.initials: KK
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D019976:Cloning, Organism / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D019076:Transgenes
chemical_list: D008164:Luminescent Proteins; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins; D004815:Epidermal Growth Factor