Regulation of the human high affinity IgE receptor beta-chain gene expression via an intronic element.
The high affinity IgE receptor, FcepsilonRI, is a key regulatory molecule in the allergic reaction. By screening for cis-acting elements over the entire region of the human FcepsilonRI beta-chain gene, a sequence located in the fourth intron was revealed to serve as a repressor element. This element was recognized by a transcription factor, myeloid zinc finger protein 1 (MZF-1). Introduction of MZF-1 antisense inhibited the suppressive effect of the element on the beta-chain promoter and increased the mRNA for the beta-chain in KU812 cells, indicating that MZF-1 repressed human FcepsilonRI beta-chain gene expression via the element in the fourth intron. Furthermore, it was suggested that a cofactor binding with MZF-1, whose expression level was different among the cell types, was required for transcriptional repression by MZF-1.
Takahashi
Kyoko
K
Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, 30-1 Oyaguchi, Kami-machi, Itabashi-ku, Tokyo 173-8610, Japan.
Nishiyama
Chiharu
C
Hasegawa
Masanari
M
Akizawa
Yushiro
Y
Ra
Chisei
C
D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007438:Introns / Q000276:immunology*; D019169:Jurkat Cells; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors; D008222:Lymphokines / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D029584:Prostatic Secretory Proteins; D011485:Protein Binding / Q000276:immunology; D021122:Protein Subunits / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D017455:Receptors, IgE / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000276:immunology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation / Q000276:immunology
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D008222:Lymphokines; C085969:MZF1 protein, human; D016376:Oligonucleotides, Antisense; D029584:Prostatic Secretory Proteins; D021122:Protein Subunits; D017455:Receptors, IgE; D012097:Repressor Proteins; D014157:Transcription Factors; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors