title: Strictly polyphosphate-dependent glucokinase in a polyphosphate-accumulating bacterium, Microlunatus phosphovorus.
abstract: ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-6-P(i) from glucose and polyP, but it could not phosphorylate glucose with ATP. The polyP-GK was most closely related to the polyP/ATP-GK of Mycobacterium tuberculosis.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, Hiroshima 739-8530, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Tanaka
authors.forename: Sun-Og
authors.initials: SO
authors.lastname: Lee
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Hamaoka
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
authors.forename: Noboru
authors.initials: N
authors.lastname: Takiguchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D005941:Glucokinase / Q000235:genetics / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011122:Polyphosphates / Q000378:metabolism*; D011423:Propionibacteriaceae / Q000201:enzymology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D011122:Polyphosphates; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; D005941:Glucokinase