title: Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates in the Archangel prison in Russia: predominance of the W-Beijing clone family.
abstract: Prisons play a significant role in the epidemiology of drug-resistant tuberculosis. A total of 114 Mycobacterium tuberculosis isolates recovered from patients in the Archangel prison (Archangel, Russia) in 2001 were studied using restriction fragment-length polymorphism analysis and spoligotyping. Drug susceptibility was analyzed by the radiometric broth method (BACTEC; Becton Dickinson Diagnostic Systems). According to genotyping studies, 87 (76.3%) of the isolates belonged to the W-Beijing family. Nearly all (96.6%) W-Beijing isolates were part of a cluster, whereas only 25.9% of the other isolates were clustered (P<.001). The largest cluster comprised 43 patients. Multidrug resistance was high among new (34.0%) and previously treated (55.0%) cases. Resistance to ethambutol (OR, 3.4; 95% CI, 1.0-12.7; P=.03) and streptomycin (OR, 4.2; 95% CI, 1.5-11.6; P=.001) was significantly associated with infection with W-Beijing isolates. Tuberculosis due to drug-resistant W-Beijing isolates is a major problem in the Archangel prison.
authors.affiliation: Division of Infectious Disease Control, Norwegian Institute of Public Health. o.s.toungoussova@samfunnsmed.uio.no
authors.forename: Olga S
authors.initials: OS
authors.lastname: Toungoussova
authors.forename: Andrey
authors.initials: A
authors.lastname: Mariandyshev
authors.forename: Gunnar
authors.initials: G
authors.lastname: Bjune
authors.forename: Per
authors.initials: P
authors.lastname: Sandven
authors.forename: Dominique A
authors.initials: DA
authors.lastname: Caugant
mesh_terms: D000293:Adolescent; D000328:Adult; D000904:Antibiotics, Antitubercular / Q000378:metabolism / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D004977:Ethambutol / Q000378:metabolism / Q000627:therapeutic use; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000378:metabolism / Q000627:therapeutic use; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011329:Prisoners; D012426:Russia / Q000453:epidemiology; D013045:Species Specificity; D013307:Streptomycin / Q000378:metabolism / Q000627:therapeutic use; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000453:epidemiology* / Q000235:genetics* / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D004977:Ethambutol; D007538:Isoniazid; D013307:Streptomycin