title: Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line.
abstract: Human embryonic stem (hES) cells have been traditionally cultured on primary mouse embryonic fibroblasts (PMEFs). However, though STO cells have some advantages over PMEFs and human embryonic fibroblasts (hEFs) as feeder cells, they have never been used as feeder cells to establish hES cell lines. In this study, three hES cell lines (Miz-hES1, Miz-hES2, and Miz-hES3) were established from inner cell masses (ICM), using STO as feeder cells. The three hES cell lines had normal karyotypes and expressed high levels of alkaline phosphatase (AP), cell surface markers (SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), and transcription factor Oct-4. After culture on STO cells for 2 yr, hES cells maintained the potential to form derivatives of all three embryonic germ layers. Our results show that STO feeder cells have the potential to support the establishment and maintenance of hES cell lines. In addition, our results suggest that laminin may play an important role in maintaining the undifferentiated proliferation of hES cells.
authors.affiliation: Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Kangseo-ku, Seoul 157-280, Korea.
authors.forename: Jong Hyuk
authors.initials: JH
authors.lastname: Park
authors.forename: Sun Jong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Oh
authors.forename: Shin Yong
authors.initials: SY
authors.lastname: Moon
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Roh
authors.forename: Chul Geun
authors.initials: CG
authors.lastname: Kim
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Yoon
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface; D015295:Antigens, Tumor-Associated, Carbohydrate; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004268:DNA-Binding Proteins / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008810:Mice, Inbred C57BL; D050814:Octamer Transcription Factor-3; D011509:Proteoglycans; D055746:Stage-Specific Embryonic Antigens; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014157:Transcription Factors
chemical_list: D000954:Antigens, Surface; D015295:Antigens, Tumor-Associated, Carbohydrate; D015415:Biomarkers; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D006028:Glycosphingolipids; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C494174:Pou5f1 protein, mouse; D011509:Proteoglycans; D055746:Stage-Specific Embryonic Antigens; C107064:TRA-1-60 antigen, human; D014157:Transcription Factors; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4; D000469:Alkaline Phosphatase