Anti-Herpes simplex virus activity of Bidens pilosa and Houttuynia cordata.
The present study evaluated the antiviral activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb., using cytotoxicity test with XTT-based colorimetric assay. BCC-1/KMC cells were infected with herpes simplex virus (HSV) and then were cultured with hot water extract of B. pilosa (HWBP) or H. cordata (HWHC). Results showed that HWBP significantly inhibited the replication of HSV at a concentration of 100 microg/ml (11.9% for HSV-1, p < 0.01; 19.2% for HSV-2, p < 0.005), whereas HWHC had the same effect at a concentration of 250 microg/ml (10.2% for HSV-1, p < 0.05; 32.9% for HSV-2, p < 0.005). The ED50 of HSV type 1 (HSV-1) and HSV type 2 (HSV-2) for HWBP was 655.4 microg/ml and 960 microg/ml respectively, for HWHC it was 822.4 microg/ml and 362.5 microg/ml respectively. Both drugs had selective indexes above 1.04. H. cordata had better effect against HSV-2 than HSV-1, and had a low ED50 against HSV-2. We suggest that H. cordata might be a useful medicinal plant against infection of HSV-2.
Chiang
Lien-Chai
LC
Department of Microbiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Chang
Jung-San
JS
Chen
Chi-Chain
CC
Ng
Lean-Teik
LT
Lin
Chun-Ching
CC
D000998:Antiviral Agents / Q000494:pharmacology*; D031176:Bidens; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D003124:Colorimetry; D019610:Cytoprotection / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006561:Herpes Simplex / Q000188:drug therapy*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D052859:Houttuynia; D006801:Humans; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract; C442780:plant extract, Bidens pilosa