Motifs in Schizosaccharomyces pombe ars3002 important for replication origin activity in Saccharomyces cerevisiae.
Ars3002 is an efficient single-copy replication origin in the fission yeast, Schizosaccharomyces pombe. In a previous study, we tested the effects of consecutive approximately 50-bp deletions throughout ars3002 on the replication efficiency of those origins in S. pombe. Here we report the results of our use of the same approximately 50-bp deletions to test the hypothesis that some of the cis-acting sequences important for replication origin activity in fission yeast might be conserved in the evolutionarily distant budding yeast, Saccharomyces cerevisiae. We found that in most cases there was no correlation between the effects of particular mutations in S. pombe and in S. cerevisiae. We conclude that it is unlikely that any of the cis-acting sequences recognised by homologous replication proteins is conserved between these two yeast species.
Antunes
Daiane Felberg
DF
Setor de Biologia Molecular-LIKA, Universidade Federal de Pernambuco. Av. Moraes Rego, s/n, Recife PE 50670-901, Brazil.
Kim
Soo-Mi
SM
Huberman
Joel A
JA
de Morais
Marcos Antonio
MA
D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics*; D019143:Evolution, Molecular; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D014170:Transformation, Genetic