title: Sesquiterpene lactone from Wunderlichia crulsiana inhibits the respiratory burst of leukocytes triggered by distinct biochemical pathways.
abstract: The sesquiterpene lactone tubiferin was chemically purified from the brazilian native plant Wunderlichia crulsiana and identified by NMR and GC/MS data. Its ability to inhibit the respiratory burst of peritoneal inflammatory polymorphonuclear leukocytes (PMN) stimulated upon addition of phorbol miristate acetate (PMA), opsonized zymosan (OZ), and N-formyl-methionyl-leucyl-phenylalanine (fMLP) was evaluated. The tubiferin inhibition was more pronounced when PMN were stimulated through the protein kinase C pathway (PMA) compared to the alternative complement pathway (OZ). The inhibition when PMN were triggered by a chemoattractant stimulus (fMLP) was similar to that achieved with OZ-stimulated phagocytes. Tubiferin showed dose-dependent effects on the PMN respiratory burst triggered by the three different substances, and also decreased substantially the carrageenan-induced mice paw edema.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Cecilia Veronica
authors.initials: CV
authors.lastname: Nuñez
authors.forename: Fabíola Maria
authors.initials: FM
authors.lastname: Zacheu
authors.forename: Ernani
authors.initials: E
authors.lastname: Pinto
authors.forename: Nídia Franca
authors.initials: NF
authors.lastname: Roque
authors.forename: Pio
authors.initials: P
authors.lastname: Colepicolo
authors.forename: Maísa Ribeiro Pereira Lima
authors.initials: MR
authors.lastname: Brigagão
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry; D001938:Brazil; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D006614:Hindlimb; D007213:Indomethacin / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D026082:Singlet Oxygen / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; C477275:tubiferin; D026082:Singlet Oxygen; D007213:Indomethacin