[Study on the transcriptional activation of MTS1 gene beta promoter].
OBJECTIVE
To investigate the effect of MTS1 gene beta promoter transcriptional activation in T-cell acute lymphoblastic leukemia (T-ALL) cell lines and identify the fragment with transcriptional activation.


METHODS
Seven pGL3 recombinant plasmids with the same 3'-end transcriptional start site but the different 5'sequences were constructed by gene recombinant technique and transfected into Jurkat cell line which is biallelic deletion of MTS1 gene by transient transfection. Luciferase report gene was detected to observe beta promoter transcriptional activation.


RESULTS
Seven pGL3 recombinant plasmids containing different fragments of beta promoter were obtained, all of them showed transcriptional activation in Jurkat cell line. Among them, the 0.38 kb fragment cut by SacII-SacI is fundamental in transcription.


CONCLUSIONS
MTS1 gene beta promoter can be activated in Jurkat cell line.
Feng
Wen-li
WL
Department of Clinical Hematology, Chongqing Medical University, Chongqing 400016, China.
Liu
Xing
X
Tu
Zhi-guang
ZG
Huang
Zong-gan
ZG
D019942:Genes, p16; D006801:Humans; D019169:Jurkat Cells; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection