title: [Subclasses of serum HDL in hyperlipidemia].
abstract: OBJECTIVE
  To detect the change of composition and ratio of serum HDL subclasses and explore the relationship between these changes and the plasma lipid level in patients with hyperlipidemia.
  
  
  METHODS
  The subclasses of serum HDL in 168 patients with hyperlipidemia were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.
  
  
  RESULTS
  Compared to the healthy controls, the pre-beta 1 HDL, pre-beta 2 HDL, HDL3c, HDL3b, and HDL3a increased significantly (P < 0.01, P < 0.01, P < 0.05, P < 0.01 and P < 0.01), while the HDL2a and HDL2b decreased significantly (P < 0.01) in the patients with hyperlipidemia. The pre-beta 1 HDL and HDL3b were significantly higher (P < 0.01) while the HDL2a and HDL2b were significantly lower (P < 0.01) in men than in women with hyperlipidemia. The pre-beta 1 HDL was significantly higher (P < 0.05) while the HDL2b was significantly lower (P < 0.05) in the male control group than in the female control group. In the patients with hyperlipidemia, the serum TG was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.583, 0.196, 0.130, and 0.370 respectively) and negatively correlated with HDL2a and HDL2b (r = -0.293 and -0.456 respectively); The serum TC was positively correlated with pre-beta 1 HDL and HDL3b (r = 0.348 and 0.219); The serum LDL-C was positively correlated with HDL3b (r = 0.187); The serum HDL-C was negatively correlated with pre-beta 1 HDL and HDL3a (r = -0.354 and -0.292 respectively) and positively correlated with HDL2a and HDL2b (r = 0.254 and 0.256); The TG/HDL-C ratio was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.593, 0.225, 0.123, and 0.394) and negatively correlated with HDL2a and HDL2b (r = -0.317 and -0.459).
  
  
  CONCLUSIONS
  The particle of HDL in the patients with hyperlipidemia shows a general shift towards smaller size, which indicates that the maturation of HDL could be abnormal in patients with hyperlipidemia. The change of subclasses of HDL in the patients with hyperlipidemia might be associated with coronary artery diseases.
authors.affiliation: Apolipoprotein Research Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xu
authors.forename: Mingde
authors.initials: M
authors.lastname: Fu
authors.forename: Bingwen
authors.initials: B
authors.lastname: Liu
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Xu
authors.forename: Jia
authors.initials: J
authors.lastname: Yao
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood; D001161:Arteriosclerosis / Q000209:etiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D053442:High-Density Lipoproteins, Pre-beta; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000150:complications; D008075:Lipoproteins, HDL / Q000097:blood* / Q000145:classification; D008297:Male; D008875:Middle Aged
chemical_list: D016632:Apolipoprotein A-I; D053442:High-Density Lipoproteins, Pre-beta; D008075:Lipoproteins, HDL