[BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats].
OBJECTIVE
To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.


METHODS
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.


RESULTS
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/8, 1/5, 0/5, 0/2, 0/1 respectively at 26th week after implantation. Histomorphometrical analysis showed much more new bony callus including cortical bone formed in group I than those of other groups. The compression strength of the implanted bone substitute of group I is significantly higher than those of group II and III.


CONCLUSIONS
The tissue-engineered bone substitute loaded with human BMP-2 gene transfected BMSC can repair diaphyseal segmental bone defect of large animal (goat).
Dai
Ke-rong
KR
Department of Orthopaedics, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Xu
Xiao-liang
XL
Tang
Ting-ting
TT
Zhu
Zhen-an
ZA
Yu
Chao-feng
CF
Xu
Min
M
Zhu
Liu-long
LL
Hao
Yong-qiang
YQ
Lou
Jue-ren
JR
D000818:Animals; D001696:Biomechanical Phenomena; D001847:Bone Diseases / Q000628:therapy*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D015316:Genetic Therapy; D006041:Goats; D045164:Mesenchymal Stem Cell Transplantation; D013977:Tibia; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta; D014945:Wound Healing
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta