Enhanced brain levels of 8,12-iso-iPF2alpha-VI differentiate AD from frontotemporal dementia.
OBJECTIVE
To quantify the isoprostane 8,12-iso-iPF2alpha-VI in brain tissues obtained from patients with AD, patients with frontotemporal dementia (FTD), and controls.


BACKGROUND
Enhanced brain oxidative stress with secondary damage to cellular macromolecules may play a role in the pathogenesis of AD and FTD. The isoprostane 8,12-iso-iPF2alpha-VI is a specific and sensitive marker of in vivo lipid peroxidation and is increased in AD.


METHODS
Levels of this isoprostane were determined by gas chromatography/negative ion chemical ionization mass spectrometry.


RESULTS
Levels of 8,12-iso-iPF2alpha-VI were markedly elevated in both frontal and temporal cortex of AD brains compared to the corresponding areas of FTD and controls. No significant difference in brain 8,12-iso-iPF2alpha-VI levels for any regions considered was observed between FTD and controls.


CONCLUSIONS
Lipid peroxidation is a feature of AD, but not FTD. The generation of 8,12-iso-iPF(2alpha)-VI in the brain is not a general or final common pathway of neurodegeneration, but may be relatively specific for disease processes in AD and not FTD.
Yao
Y
Y
Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia 19104, USA.
Zhukareva
V
V
Sung
S
S
Clark
C M
CM
Rokach
J
J
Lee
V M-Y
VM
Trojanowski
J Q
JQ
Praticò
D
D
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers; D001923:Brain Chemistry; D003704:Dementia / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009410:Nerve Degeneration; D018384:Oxidative Stress; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020774:Pick Disease of the Brain / Q000378:metabolism / Q000473:pathology; D013494:Supranuclear Palsy, Progressive / Q000378:metabolism / Q000473:pathology
C115626:8,12-iso-isoprostane F2alpha-VI; D015415:Biomarkers; D015237:Dinoprost