Molecular identification of Erysipelothrix isolates from the tonsils of healthy cattle by PCR.
For 79 isolates from the tonsils of healthy cattle identified as Erysipelothrix by cultivation, biochemical and serological tests, genotypic identification was performed by polymerase chain reaction (PCR) using four species-specific sets of oligonucleotide primers (ER1F-ER1R, ER2F-ER2R, ER3F-ER3R and ER4F-ER4R). The results of PCR for 79 bovine isolates were compared with those of serological typing. For 19 isolates, serotyping and genotyping results were the same. PCR allowed for the identification of 36 untypable isolates as Erysipelothrix species, strain 1. Serotyping and genotyping results of the remaining 24 isolates were different. Supplemental tests are frequently needed for Erysipelothrix identification.
Hassanein
Raafat
R
Department of Veterinary Microbiology, Nippon Veterinary and Animal Science University, 7-1 Kyonan-cho, 1 Chome, Musashino-shi, Tokyo 180-8602, Japan.
Sawada
Takuo
T
Kataoka
Yasushi
Y
Gadallah
Asmaa
A
Suzuki
Yoshikazu
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004888:Erysipelothrix / Q000235:genetics* / Q000302:isolation & purification; D004889:Erysipelothrix Infections / Q000382:microbiology*; D014066:Palatine Tonsil / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity
D004269:DNA, Bacterial