[Determination of deoxyribonucleic acid with rosaniline by a resonance light scattering method].
The resonance light scattering (RLS) spectra of Rosaniline with DNA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak ofat 485 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg.L-1 with the correlation coefficient of 0.9980. The detection limit is 14.2 ng.mL-1. The relative standard deviation(n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.
Liu
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Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
Chen
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XM
Xiang
H Y
HY
Li
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S
Xia
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Liu
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AX
D001288:Attention; D002138:Calibration; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D012394:Rosaniline Dyes; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D012394:Rosaniline Dyes; C025485:basic fuchsin; D004247:DNA