Clustering versus random segregation of plasmids lacking a partitioning function: a plasmid paradox?
Plasmids lacking a functional partition system are randomly distributed to the daughter cells; plasmid-free daughter cells are formed with a frequency of (1/2)2n per cell and cell generation where 2n is the (average) copy number at cell division. Hence, the unit of segregation is one plasmid copy. However, plasmids form clusters in the cells. A putative solution to this potential paradox is presented: one plasmid copy at a time is recruited from the plasmid clusters to the replication factories that are located in the cell centres. Hence, replication offers the means of declustering that is necessary in a growing host population. The daughter copies diffuse freely and each copy may with equal probability end up in either of the two cell halves. In this way, the random segregation of the plasmids is coupled to replication and occurs continuously during the cell cycle, and is not linked to cell division. The unit of segregation is the plasmid copy and not the plasmid clusters. In contrast, the two daughters of a Par+ plasmid are directed in opposite directions by the plasmid-encoded partition system, thereby assuring that each daughter cell receives the plasmid.
Nordström
Kurt
K
Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, P.O. Box 596, Uppsala S-751 24, Sweden. kurt.nordstrom@icm.uu.se
Gerdes
Kenn
K
D001426:Bacterial Proteins; D020090:Chromosome Segregation / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics*
D001426:Bacterial Proteins; C052883:chromosome partition proteins, bacterial