title: NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity.
abstract: The Mixed Stain Study 3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the linkages connecting the measurement of the concentration of DNA ([DNA]) to the observed STR multiplex signal intensities. There is a causal relationship between [DNA] measurement accuracy and the efficiency of STR multiplex analysis. There are no intrinsic measurement performance differences among the [DNA] measurement technologies reported. However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems.
authors.affiliation: Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
authors.forename: Margaret C
authors.initials: MC
authors.lastname: Kline
authors.forename: David L
authors.initials: DL
authors.lastname: Duewer
authors.forename: Janette W
authors.initials: JW
authors.lastname: Redman
authors.forename: John M
authors.initials: JM
authors.lastname: Butler
mesh_terms: D004247:DNA / Q000032:analysis*; D004586:Electrophoresis / Q000295:instrumentation; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D020080:Tandem Repeat Sequences
chemical_list: D004247:DNA