title: Alpha-synuclein expression localizes to the Golgi apparatus in bovine adrenal medullary chromaffin cells.
abstract: Alpha-synuclein (alphaS) is a protein of unknown function linked to Parkinson's disease. We examined alphaS expression in adrenal medullary chromaffin cells. Immunocytochemistry showed expression of alphaS in the Golgi apparatus and vesicles, consistent with its putative role in vesicular function within synapses, and with O-linked glycosylation of alphaS in autosomal-recessive Parkinson's disease. The chromaffin cell culture system offers advantages in studying the role of alphaS in vesicular trafficking and secretion.
authors.affiliation: Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, P.O. Box 913, Dunedin, New Zealand. peggy.tompkins@stonebow.otago.ac.nz
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Tompkins
authors.forename: Wei Ping
authors.initials: WP
authors.lastname: Gai
authors.forename: Shirley
authors.initials: S
authors.lastname: Douglas
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Bunn
mesh_terms: D000313:Adrenal Medulla / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D051843:Synucleins; D051844:alpha-Synuclein
chemical_list: D009419:Nerve Tissue Proteins; C497606:Snca protein, rat; D051843:Synucleins; D051844:alpha-Synuclein