Changes in salivary cortisol concentrations during a 24-hour period in dogs.
The purpose of this investigation was to clarify whether salivary cortisol secretion in dogs had a circadian rhythm. Saliva sampling during a 24-hour period was performed in 4 non-consecutive days. Eight adult beagle dogs (4 males and 4 females) were divided into 4 groups, and 2 dogs (1 male and 1 female) were used for each repetition. Saliva samples were taken at 1 h intervals from 9:00 a. m. to 9:00 a. m. of the following day. Salivary cortisol concentrations were determined using enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary cortisol, and the differences among salivary cortisol concentrations measured every hour were not demonstrated during a 24-hour period in dogs.
Koyama
T
T
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan. atsushis@obihiro.ac.jp
Omata
Y
Y
Saito
A
A
D000818:Animals; D002940:Circadian Rhythm; D004285:Dogs / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006854:Hydrocortisone / Q000032:analysis*; D008297:Male; D012463:Saliva / Q000737:chemistry*
D006854:Hydrocortisone