title: Gastrointestinal cryptosporidiosis in a puppy.
abstract: An 8-week-old female Yorkshire terrier with a history of weakness, diarrhea and intestinal isosporiasis was euthanized and a post mortem examination performed. Histologically, there was severe gastrointestinal cryptosporidiosis, severe intestinal isosporiasis and thymic lymphoid depletion. PCR revealed visible bands for the actin and 18S rRNA genes but not for the acetyl CoA synthetase gene for Cryptosporidium spp. The PCR product for the actin gene was sequenced and found to have a 97.6-99.8% similarity to that of Cryptosporidium canis. To our knowledge, this is the first report of gastric cryptosporidiosis in a canine.
authors.affiliation: The University of Georgia, College of Veterinary Medicine, Veterinary Diagnostic and Investigational Laboratory, Tifton, GA 31793, USA. dmiller@tifton.uga.edu
authors.forename: D L
authors.initials: DL
authors.lastname: Miller
authors.forename: A
authors.initials: A
authors.lastname: Liggett
authors.forename: Z A
authors.initials: ZA
authors.lastname: Radi
authors.forename: L O
authors.initials: LO
authors.lastname: Branch
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry / Q000235:genetics; D000199:Actins / Q000737:chemistry / Q000235:genetics; D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D005260:Female; D005767:Gastrointestinal Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000469:parasitology / Q000473:pathology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
chemical_list: D000199:Actins; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D000105:Acetyl Coenzyme A