Short hairpin RNAs induced RNA interference in human cells.
OBJECTIVE
RNA interference (RNAi) is an evolutionarily conserved posttranscriptional gene silencing, in which the introduction of double-stranded RNA into a cell leads to specific suppression of gene expression. RNAi has become an important tool for gene function studies. The aim of this study was to induce RNAi in mammalian cells by short hairpin RNAs (shRNAs) generated from a DNA vector, therefore, to provide a new approach for gene function analysis.


METHODS
Dual Luciferase System was used to assess the intracellular effect of the shRNAs generated from a DNA vector. The inhibitory effect of this DNA vector-driven shRNAs at different condition was measured.


RESULTS
shRNAs generated from the DNA vector induced RNAi in human cells, and suppressed gene expression in a sequence-specific manner. The inhibitory effect was highly related to the target sites.


CONCLUSIONS
shRNAs induced RNAi in human cells and suppressed gene expression in a sequence-specific manner. This approach may be used for gene function study.
Chen
Yang-Chao
YC
Department of Biochemistry, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Song
Chao
C
Luo
Chao-Quan
CQ
D020868:Gene Silencing; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D034622:RNA Interference