Establishment of embryonic neuroepithelial cell lines exhibiting an epiplastic expression pattern of region specific markers.
Neuroepithelial b2T cells were derived from the hindbrain and the spinal cord of mouse transgenic embryos, which expressed SV40 T antigen under the control of a Hoxb2 enhancer. Strikingly, b2T cell lines of either origin exhibit a very similar gene expression pattern, including markers of the hindbrain and the spinal cord, such as Hox genes, but not of more anterior cephalic regions. In addition, the broad expression pattern of b2T cells, probably linked to culture conditions, appeared to be appropriately modulated when the cells were reimplanted at different longitudinal levels into chick host embryos, suggesting that these cells are responsive to exogenous signalling mechanisms. Further support for these allegations was obtained by culturing b2T cells in defined medium and by assessing the expression of Krox20, an odd-numbered rhombomere marker, which appeared to be modulated by a complex interplay between FGF, retinoic acid (RA), and noggin. With respect to these as yet unique properties, b2T cells constitute an original alternative tool to in vivo models for the analysis of molecular pathways involved in the patterning of the neural tube.
Nardelli
Jeannette
J
INSERM U368, Ecole Normale Supérieure, Paris, France. nardelli@idf.ext.jussieu.fr
Catala
Martin
M
Charnay
Patrick
P
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D030161:Avian Proteins; D019521:Body Patterning / Q000502:physiology*; D002352:Carrier Proteins; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D051767:Early Growth Response Protein 2; D004622:Embryo, Mammalian; D004742:Enhancer Elements, Genetic / Q000502:physiology; D004848:Epithelium / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018398:Homeodomain Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051258:MafB Transcription Factor; D008636:Mesencephalon / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis; D009419:Nerve Tissue Proteins; D064231:Nestin; D009432:Neural Crest; D015513:Oncogene Proteins / Q000378:metabolism; D016548:Prosencephalon / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology; D015347:RNA Probes / Q000378:metabolism; D012249:Rhombencephalon / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D000099176:Noggin Protein
D000952:Antigens, Polyomavirus Transforming; D000970:Antineoplastic Agents; D030161:Avian Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; D005346:Fibroblast Growth Factors; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D051258:MafB Transcription Factor; D009419:Nerve Tissue Proteins; D064231:Nestin; D015513:Oncogene Proteins; D011506:Proteins; D015347:RNA Probes; D015534:Trans-Activators; D014157:Transcription Factors; D014212:Tretinoin; D000099176:Noggin Protein; C497327:Egr2 protein, mouse; C490406:Mafb protein, mouse; C577485:Nes protein, mouse; C091157:maf-B protein, chicken