title: Direct comparison of demyelinating disease induced by the Daniel's strain and BeAn strain of Theiler's murine encephalomyelitis virus.
abstract: We compared CNS disease following intracerebral injection of SJL mice with Daniel's (DA) and BeAn 8386 (BeAn) strains of Theiler's murine encephalomyelitis virus (TMEV). In tissue culture, DA was more virulent then BeAn. There was a higher incidence of demyelination in the spinal cords of SJL/J mice infected with DA as compared to BeAn. However, the extent of demyelination was similar between virus strains when comparing those mice that developed demyelination. Even though BeAn infection resulted in lower incidence of demyelination in the spinal cord, these mice showed significant brain disease similar to that observed with DA. There was approximately 100 times more virus specific RNA in the CNS of DA infected mice as compared to BeAn infected mice. This was reflected by more virus antigen positive cells (macrophages/microglia and oligodendrocytes) in the spinal cord white matter of DA infected mice as compared to BeAn. There was no difference in the brain infiltrating immune cells of DA or BeAn infected mice. However, BeAn infected mice showed higher titers of TMEV specific antibody. Functional deficits as measured by Rotarod were more severe in DA infected versus BeAn infected mice. These findings indicate that the diseases induced by DA or BeAn are distinct.
authors.affiliation: Department of Immunology, Mayo Medical and Graduate Schools, Rochester, Minn, USA.
authors.forename: Laurie J
authors.initials: LJ
authors.lastname: Zoecklein
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Pavelko
authors.forename: Jeff
authors.initials: J
authors.lastname: Gamez
authors.forename: Louisa
authors.initials: L
authors.lastname: Papke
authors.forename: Dorian B
authors.initials: DB
authors.lastname: McGavern
authors.forename: Daren R
authors.initials: DR
authors.lastname: Ure
authors.forename: M Kariuki
authors.initials: MK
authors.lastname: Njenga
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Johnson
authors.forename: Shunya
authors.initials: S
authors.lastname: Nakane
authors.forename: Moses
authors.initials: M
authors.lastname: Rodriguez
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000941:Antigens / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D001921:Brain / Q000473:pathology / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line / Q000821:virology; D020805:Central Nervous System Viral Diseases; D006224:Cricetinae; D003711:Demyelinating Diseases / Q000209:etiology / Q000473:pathology* / Q000821:virology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000592:standards; D020233:Gait Disorders, Neurologic / Q000821:virology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007120:Immunochemistry / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000473:pathology / Q000821:virology; D051379:Mice; D008815:Mice, Inbred Strains / Q000821:virology; D011051:Poliomyelitis / Q000150:complications*; D012313:RNA / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012196:Review Literature as Topic; D013045:Species Specificity; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology / Q000821:virology; D017945:Theilovirus / Q000472:pathogenicity*; D013997:Time Factors; D010948:Viral Plaque Assay / Q000379:methods
chemical_list: D000941:Antigens; D000943:Antigens, Differentiation; D036022:Capsid Proteins; D005904:Glial Fibrillary Acidic Protein; C442556:VP2 protein, Theilovirus; C036763:monocyte-macrophage differentiation antigen; D012313:RNA