7-hydroperoxycholesterol and oxysterols as indices of oxidative stress: chronic ethanol feeding and rat skeletal muscle.
The present study is undertaken to determine if ethanol affects 7-hydroperoxycholesterol or oxysterols in rat skeletal muscle after chronic ethanol feeding. Wistar rats were fed a liquid diet containing ethanol as 35% of total calories. After 6 weeks, soleus (Type I fibre-predominant) and plantaris (Type II fibre-predominant) skeletal muscles were dissected out. We measured 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ol (7alpha-OOH and 7beta-OOH) as well as 7alpha- and 7beta-hydroxycholesterol (7alpha-OH and 7beta-OH) and 3beta-hydroxycholest-5-en-7-one (7-keto). We found that in response to chronic alcohol feeding, there were significant increases in soleus 7alpha-OH (P=0.0005), 7beta-OH (P=0.0005) and 7-keto (P=0.0007), but in the plantaris, 7beta-OH increased (P=0.0418). Their elevation in chronic experimental alcoholism, together with increases in cholesterol hydroperoxides, may possibly represent evidence of increased oxidative stress.
Adachi
Junko
J
Department of Legal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kudunoki-cho, Chuo-ku, Kobe 650-0017, Japan. adachi@med.kobe-u.ac.jp
Fujita
Tetsuo
T
Kudo
Risa
R
Asano
Migiwa
M
Nurhantari
Yudha
Y
Ueno
Yasuhiro
Y
D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000494:pharmacology*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D007653:Ketocholesterols / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D007653:Ketocholesterols; D008054:Lipid Peroxides; C041011:cholesterol 7-hydroperoxide; D000431:Ethanol; D002784:Cholesterol