title: [BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats].
abstract: OBJECTIVE
  To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
  
  
  METHODS
  The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
  
  
  RESULTS
  X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/8, 1/5, 0/5, 0/2, 0/1 respectively at 26th week after implantation. Histomorphometrical analysis showed much more new bony callus including cortical bone formed in group I than those of other groups. The compression strength of the implanted bone substitute of group I is significantly higher than those of group II and III.
  
  
  CONCLUSIONS
  The tissue-engineered bone substitute loaded with human BMP-2 gene transfected BMSC can repair diaphyseal segmental bone defect of large animal (goat).
authors.affiliation: Department of Orthopaedics, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Ke-rong
authors.initials: KR
authors.lastname: Dai
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Xu
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Tang
authors.forename: Zhen-an
authors.initials: ZA
authors.lastname: Zhu
authors.forename: Chao-feng
authors.initials: CF
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Liu-long
authors.initials: LL
authors.lastname: Zhu
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Hao
authors.forename: Jue-ren
authors.initials: JR
authors.lastname: Lou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001847:Bone Diseases / Q000628:therapy*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D015316:Genetic Therapy; D006041:Goats; D045164:Mesenchymal Stem Cell Transplantation; D013977:Tibia; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta; D014945:Wound Healing
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta