[Study on the purification of SCAMP and the preparation of its sulfated polysaccharides].
Shark cartilage acid mucopolysaccharide (SCAMP) was extracted from cartilage by the improved method, then we can get SCAMP-F1 and SCAMP-F2 from SCAMP by sepharose-4B chromatography. It was improved that SCAMP-F2 had only one component by sepharose-4B chromatography, PAGE (polyacrylamide gel electrophoresis) and especial centrifuge. At the same time, SCAMP-F2 reacted with sulfated reagents (composed of chlorosulfonic acid and pyridine), and sulfated SCAMP-F2 was obtained. Its sulfur content is 19.6%, higher 6.1% than unsulfated SCAMP-F2, IR and 13C-NMR data showed that additional sulfates were located on the carbon-6.
Li
Dong-xia
DX
Life Science College of Nanjing Normal University, Nanjing 210097, China.
Zhang
Shuang-quan
SQ
Liu
Ping
P
D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012754:Sharks; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000737:chemistry
D011134:Polysaccharides; D013431:Sulfates