The rate constants of the reaction of hydroxyl radicals (*OH) with alcohol dehydrogenase and Glyceraldehyde-3-phosphate dehydrogenase.
The rate constants of the reactions of alcohol dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase with hydroxyl radicals were determined using the method of steady-state competitive reactions. Ethanol was used as a scavenger of hydroxyl radicals. The rate constants of the reactions of hydroxyl radicals with alcohol dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase were found to be 2.8 x 10(12) dm(3) mol(-1) s(-1), and 1.6 x 10(12) dm(3) mol(-1) s(-1), respectively.
Kowalczyk
Aleksandra
A
Department of Molecular Biophysics, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland. olakow@biol.uni.lodz.pl
Puchała
Mieczysław
M
D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000528:radiation effects; D000431:Ethanol / Q000378:metabolism; D005609:Free Radicals; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000737:chemistry* / Q000528:radiation effects; D017665:Hydroxyl Radical / Q000737:chemistry*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013816:Thermodynamics
D005609:Free Radicals; D017665:Hydroxyl Radical; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)