Identification of novel proteinase K-resistant C-terminal fragments of PrP in Creutzfeldt-Jakob disease.
The central event in the pathogenesis of prion diseases, a group of fatal, transmissible neurodegenerative disorders including Creutzfeldt-Jakob disease (CJD) in humans, is the conversion of the normal or cellular prion protein (PrPC) into the abnormal or scrapie isoform (PrPSc). The basis of the PrPC to PrPSc conversion is thought to involve the diminution of alpha-helical domains accompanied by the increase of beta structures within the PrP molecule. Consequently, treatment of PrPSc with proteinase K (PK) generates a large PK-resistant C-terminal core fragment termed PrP27-30 that in human prion diseases has a gel mobility of approximately 19-21 kDa for the unglycosylated form, and a ragged N terminus between residues 78 and 103. PrP27-30 is considered the pathogenic and infectious core of PrPSc. Here we report the identification of two novel PK-resistant, but much smaller C-terminal fragments of PrP (PrP-CTF 12/13) in brains of subjects with sporadic CJD. PrP-CTF 12/13, like PrP27-30, derive from both glycosylated as well as unglycosylated forms. The unglycosylated PrPCTF 12/13 migrate at 12 and 13 kDa and have the N terminus at residues 162/167 and 154/156, respectively. Therefore, PrP-CTF12/13 are 64-76 amino acids N-terminally shorter than PrP27-30 and are about half of the size of PrP27-30. PrP-CTF12/13 are likely to originate from a subpopulation of PrPSc distinct from that which generates PrP27-30. The finding of PrP-CTF12/13 in CJD brains widens the heterogeneity of the PK-resistant PrP fragments associated with prion diseases and may provide useful insights toward the understanding of the PrPSc structure and its formation.
Zou
Wen-Quan
WQ
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Capellari
Sabina
S
Parchi
Piero
P
Sy
Man-Sun
MS
Gambetti
Pierluigi
P
Chen
Shu G
SG
D000906:Antibodies / Q000737:chemistry; D001921:Brain / Q000378:metabolism; D002465:Cell Movement; D007562:Creutzfeldt-Jakob Syndrome / Q000378:metabolism*; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000494:pharmacology*; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D018622:PrP 27-30 Protein / Q000737:chemistry* / Q000378:metabolism; D011233:Precipitin Tests; D017096:Prion Diseases / Q000378:metabolism; D017434:Protein Structure, Tertiary
D000906:Antibodies; D018622:PrP 27-30 Protein; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D019286:Endopeptidase K