title: The prevalence and diagnostic value of specific IgE antibodies to inhalant, animal and plant food, and ficus allergens in patients with natural rubber latex allergy.
abstract: BACKGROUND
  It is well recognised that natural rubber latex allergy can be associated with serological cross-reactivity to plant allergens, especially tropical fruits and Ficus. In contrast, data on the frequency and clinical value of specific IgE antibodies against these allergens remain rare. In addition, little is known about the prevalence and diagnostic value of specific IgE antibodies to classical inhalant and animal allergens in NRL allergic patients.
  
  
  OBJECTIVE
  The purpose of this study was to investigate the prevalence, the sensitivity, and the specificity of these different specific IgE antibodies in patients suffering from NRL allergy.
  
  
  METHODS
  Serum samples of 42 NRL allergic adults were investigated. All had a history of NRL allergy confirmed by a positive skin test for latex and a positive latex-specific IgE. Samples were analysed for IgE antibodies against 9 plant food allergens (avocado, banana, chestnut, fig, kiwi, papaya, peanut, pineapple and tomato) and Ficus benjamina. A specific IgE quantification for 3 animal food allergens (codfish, cow's milk, egg's white) and 8 common inhalant allergens (Dermatophagoïdes pteronyssinus, birch pollen, timothy grass pollen, mugwort pollen, cat and dog epithelium, Aspergillus fumigatus and Cladosporium herbarum) was also performed. Because double blind placebo-controlled challenges could not be considered, for ethical reasons, patient's food allergy or immediate hypersensitivity for Ficus and inhalant allergens was documented by a standardised questionnaire. Diagnosis of atopy was based on a relevant history and the presence of a specific IgE antibody to at least one classical inhalant allergen. For some IgE determinations presence or absence of cross-reactivity was investigated by CAP-inhibition tests.
  
  
  RESULTS
  A specific IgE antibody to at least one of the investigated inhalant and animal food allergens was found in respectively 76% and 12% of the serum samples. A plant food-specific IgE antibody was observed in 88% of the serum samples, most frequently to papaya (71%) and least frequently to kiwi (17%). Twenty-nine percent of the serum samples contained Ficus-IgE. According to the questionnaire and the threshold of 0.35 kUa/L, sensitivity of the plant food IgE antibodies varied between 0% for papaya and 73% for avocado. Specificity varied between 28% for papaya and 91% for kiwi. For Ficus-IgE sensitivity was 20% and specificity 70%.
  
  
  CONCLUSIONS
  For inhalant and animal food allergens sensitivity and specificity of the IgE quantification correlated generally well with the values obtained in non-NRL allergic adults. Determination of specific IgE to the investigated plant foods and Ficus was not always a sensitive neither a specific test to establish the clinical diagnosis of this allergy.
authors.affiliation: Departement Immunologie, Allergologie en Reumatologie Universiteit Antwerpen Universiteitsplein 1, B 2610 Antwerpen, België.
authors.forename: D G
authors.initials: DG
authors.lastname: Ebo
authors.forename: C H
authors.initials: CH
authors.lastname: Bridts
authors.forename: M M
authors.initials: MM
authors.lastname: Hagendorens
authors.forename: L S
authors.initials: LS
authors.lastname: De Clerck
authors.forename: W J
authors.initials: WJ
authors.lastname: Stevens
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001530:Belgium / Q000453:epidemiology; D002417:Cattle; D015331:Cohort Studies; D015897:Comorbidity; D003429:Cross Reactions; D005260:Female; D030681:Ficus / Q000009:adverse effects*; D005500:Follow-Up Studies; D005512:Food Hypersensitivity / Q000453:epidemiology* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E; D020315:Latex Hypersensitivity / Q000453:epidemiology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016269:Milk Hypersensitivity / Q000175:diagnosis / Q000453:epidemiology; D015995:Prevalence; D018570:Risk Assessment; D012494:Sampling Studies
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E