Development of a device for selective removal of CD4+ T cells.
To control antigen (Ag)-specific immune cells is important in the treatment of autoimmune diseases. In particular, controlling the immune response of autoimmune T cells is effective in the treatment of these diseases. The development of a device that can remove CD4+ T cells specifically by extracorporeal circulation is now in progress, with the aim to deplete autoimmune T cells. We developed a removal material made of polypropylene non-woven fabrics with anti human CD4 monoclonal antibody immobilized on the surface. Using a column packed with the removal material, we succeeded in removing CD4+ T cells specifically from peripheral whole blood by direct perfusion. Moreover, CD4+ T cells can be specifically removed even from blood with lower surface antigen density by in vitro activation.
Onodera
Hirokazu
H
Research and Development Laboratory 2, Asahi Medical Co., Oita, Japan. onodera.hc@om.asahi-kasei.co.jp
Ninomiya
Kasumi
K
Yoshida
Makoto
M
Matsuo
Hidenori
H
Shibuya
Noritoshi
N
D001327:Autoimmune Diseases / Q000628:therapy; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002478:Cells, Cultured; D004867:Equipment Design; D004869:Equipment Safety; D006801:Humans; D012680:Sensitivity and Specificity