Determination of furanic compounds in traditional balsamic vinegars by ion-exclusion liquid chromatography and diode-array detection.
A method for the determination of furanic compounds in traditional balsamic vinegars is proposed. It is based on ion-exclusion chromatographic separation and diode-array detection of furans through an isocratic elution with 0.01 N phosphoric acid and 16% acetonitrile. Preliminary trials on standard compounds stability in heat-acidic conditions are also performed. In all the 19 samples analyzed, 2-furoic acid, 5 HMF, and furfural are found. No sample contains 4-hydroxy-2,5-dimethyl-3-(2H)-furanone (DHMF); 2-acetylfuran; or furfuryl alcohol. Three unknown compounds are also detected. The last eluting of these compounds is identified as 5-acethoxymethylfurfural, and, notwithstanding a partial hydrolysis in our chromatographic conditions, its quantitation can be carried out.
Chinnici
Fabio
F
Dipartimento di Scienze degli Alimenti, Università di Bologna, Via Fanin 40, 40127, Bologna, Italy. chinninci@agrsci.unibo.it
Masino
Francesca
F
Antonelli
Andrea
A
D019342:Acetic Acid / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005663:Furans / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D005663:Furans; D019342:Acetic Acid