title: Effect of insulin on the rate of hydrogen peroxide generation in mitochondria.
abstract: We studied the effect of insulin on H2O2 generation by mitochondria in rat liver and heart. Insulin markedly increased the rate of H2O2 generation, which was realized via short-term activation of mitochondrial succinate dehydrogenase. In terms of the Michaelis-Menten equation describing the dependence of H2O2 generation by mitochondria on succinate concentration (succinate dehydrogenase substrate), insulin decreased the Michaelis-Menten constant and increased the maximum rate of H2O2 generation compared to the control.
authors.affiliation: Department of General Pathology and Pathophysiology, Russian Medical Academy of Postgraduate Education, Russian Ministry of Health, Moscow. ipomytkin@mtu-net.ru
authors.forename: I A
authors.initials: IA
authors.lastname: Pomytkin
authors.forename: O E
authors.initials: OE
authors.lastname: Kolesova
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D007328:Insulin; D016877:Oxidants; D019802:Succinic Acid; D006861:Hydrogen Peroxide; D013385:Succinate Dehydrogenase