title: Early neuronal progenitor cell line expressing solely non-catalytic isoform of TrkC.
abstract: TrkC is a receptor for neurotrophin-3 that regulates development of neuronal precursors. Transduction of signals into receptor-dependent signaling pathways is mainly due to the activation of the intrinsic tyrosine kinase of the TrkC receptor. Alternative splicing of the trkC transcripts generates catalytic and non-catalytic isoforms. The non-catalytic isoform, denoted as TrkC-NC2, contains unique sequence, instead of deleted entire kinase domain. Here, we report that neural cell line MB-G, derived from brain of embryos of transgenic tsA58-SV40 mice, contains mRNA encoding TrkC-NC2 without concomitant expression of mRNA for catalytic TrkC molecule.
authors.affiliation: Laboratory of Cellular Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Matuszyk
authors.forename: Ewa
authors.initials: E
authors.lastname: Ziolo
authors.forename: Dagmara
authors.initials: D
authors.lastname: Plawiak
authors.forename: Leon
authors.initials: L
authors.lastname: Strzadala
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000378:metabolism; D002384:Catalysis; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology*; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020812:Receptor, trkC / Q000096:biosynthesis* / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D014404:Tubulin / Q000096:biosynthesis
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D014404:Tubulin; C462941:beta3 tubulin, mouse; D020812:Receptor, trkC