title: Vaccination with nontoxic mutant toxic shock syndrome toxin 1 protects against Staphylococcus aureus infection.
abstract: To investigate whether vaccination with nontoxic mutant toxic shock syndrome toxin 1 (mTSST-1) can protect against Staphylococcus aureus infection, mice were vaccinated with mTSST-1 and challenged with viable S. aureus. Survival in the mTSST-1-vaccinated group was higher, and bacterial counts in organs were significantly lower than those of control mice. Passive transfer of mTSST-1-specific antibodies also provided protection against S. aureus-induced septic death. Interferon (IFN)-gamma production in the serum samples and spleens from vaccinated mice was significantly decreased compared with that in controls, whereas interleukin-10 titers were significantly higher in vaccinated mice. IFN-gamma and tumor necrosis factor-alpha production in vitro were significantly inhibited by serum samples from mTSST-1-immunized mice but not from control mice. These results suggest that vaccination with mTSST-1 devoid of superantigenic properties provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibodies as well as by the down-regulation of IFN-gamma production.
authors.affiliation: Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan.
authors.forename: Dong-Liang
authors.initials: DL
authors.lastname: Hu
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Omoe
authors.forename: Sanae
authors.initials: S
authors.lastname: Sasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sashinami
authors.forename: Hirotake
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Shinagawa
authors.forename: Akio
authors.initials: A
authors.lastname: Nakane
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D007116:Immunization, Passive; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012772:Shock, Septic / Q000276:immunology / Q000401:mortality / Q000517:prevention & control; D013203:Staphylococcal Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D018089:Superantigens; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D011994:Recombinant Proteins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D016753:Interleukin-10; D007371:Interferon-gamma