title: Fasciola hepatica: effects of the fasciolicide clorsulon in vitro and in vivo on the tegumental surface, and a comparison of the effects on young- and old-mature flukes.
abstract: The ultrastructural changes in Fasciola hepatica induced by the fasciolicide clorsulon were assessed using scanning electron microscopy. At 8 and 44 weeks post-infection, male Sprague-Dawley rats infected with F. hepaticawere dosed orally with clorsulon at a concentration of 12.5 mg/kg and mature flukes recovered from the bile duct after 24 h, 48 h, and 72 h in both experiments. An in vitro incubation was also set up using mature fluke (8 weeks old) incubated with clorsulon for 24 h at a concentration of 10 microg/ml. After 24 h in vivo, the young-mature flukes (8 weeks old) showed significant disruption to the tegumental surface, particularly in the anterior mid-body region, where a distinct band of swelling and blebbing was evident. The band began just behind the ventral sucker and ran posteriorly along both margins. The apical cone region of the fluke was characterised by swelling and blebbing of the surface between the spines. Similar changes were evident after 48 h in vivo, but the disruption was more severe and the mid-body band had spread posteriorly. In approximately half of the specimens recovered after 72 h in vivo, widespread disruption had occurred, with sloughing of the apical membrane or the entire syncytium, over almost all of the oral cone and anterior mid-body. For all time periods, the anterior half of the fluke was more severely affected than the posterior half. No differences were seen between the dorsal and ventral surfaces. Old-mature flukes (44 weeks old) showed regionally similar, but more severe and widespread disruption than that seen in the young-mature flukes. The onset of surface changes occurred more quickly in old-mature flukes as well. Eight-week-old flukes which had been incubated for 24 h in vitro showed surprisingly little disruption, but this may be due to the method by which the drug is taken up by the fluke.
authors.affiliation: Parasite Proteomics and Therapeutics Research Group, The School of Biology and Biochemistry, The Queen's University of Belfast, BT9 7BL Belfast, Northern Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Meaney
authors.forename: I
authors.initials: I
authors.lastname: Fairweather
authors.forename: G P
authors.initials: GP
authors.lastname: Brennan
authors.forename: L S L
authors.initials: LS
authors.lastname: McDowell
authors.forename: A B
authors.initials: AB
authors.lastname: Forbes
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects / Q000648:ultrastructure; D005210:Fasciola hepatica / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*; D005211:Fascioliasis / Q000188:drug therapy* / Q000469:parasitology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D013424:Sulfanilamides / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D013424:Sulfanilamides; C015675:clorsulon