[Determination of Na, K and Ca in brains of less white mouse by AAS].
Sodium, potassium and calcium in brain of less white mouse were determined using an atomic absorption spectrometry. The sodium was atomized by air-town-gas flame, and the potassium and calcium were atomized by air-acetylene flame. The brains took from 46 less white mice which came from 4 sets of contrast samples. The brain (0.05-0.1 g) after drying up was digested by a nitric-perchloric acid system. The interference effect of phosphor in the brain on the calcium was overcome by adding in lanthanum chloride. Sodium and potassium in the brain itself were used as deionization agents for determining calcium. Cesium chloride was used as the deionization agent for determining sodium and potassium. The experimental results showed that the RSD (n = 6) was 0.886% for sodium, 0.691% for potassium and 0.824% for calcium, and the addition standard recovery (ASR) (n = 3) was 97.5%-102.4% for sodium, 100.3%-104.0% for potassium and 96.0%-103.2% for calcium, respectively.
Yang
Jian-nan
JN
Department of Chemical Engineering, Jiangsu Institute of Petrochemical Technology, Changzhou 213016, China.
Luo
Shi-ping
SP
D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000032:analysis*; D051379:Mice; D011188:Potassium / Q000032:analysis*; D012964:Sodium / Q000032:analysis*; D013054:Spectrophotometry, Atomic
D012964:Sodium; D011188:Potassium; D002118:Calcium