Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray.
To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
Khadijah
Siti
S
Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.
Neo
Soek Ying
SY
Hossain
M S
MS
Miller
Lance D
LD
Mathavan
S
S
Kwang
Jimmy
J
D000818:Animals; D004267:DNA Viruses / Q000235:genetics*; D033364:Decapoda / Q000821:virology*; D005814:Genes, Viral; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017735:Virus Latency / Q000235:genetics*
D012367:RNA, Viral