A simplified method for purification of annexin V from human placenta.
A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.
Poghosyan
G G
GG
Institute of Biochemistry, NAS of Armenia, Yerevan, Republic of Armenia.
Melkonyan
V Z
VZ
Mikaelyan
M V
MV
Gasparyan
V K
VK
D017304:Annexin A5 / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy
D017304:Annexin A5