title: A glucose biosensor employing a stable artificial peroxidase based on ruthenium purple anchored cinder.
abstract: Iron-enriched industrial waste cinder (CFe*) has been recycled for efficient and stable anchoring of Ru(CN)6(4-) to the formation of a hybrid ruthenium purple complex. The cinder/ruthenium purple hybrid-modified carbon paste electrode (designated as CPE/CFe*-RP) was worked out for hydrodynamic analysis of H2O2 at a low detecting potential of 0.0 V versus Ag/AgCl in pH 7 ammonium buffer solution. The highly active, selective, and stable electrocatalytic system with a function similar to peroxidase enzyme shows a linear calibration curve up to 0.8 mM H2O2 at a rotation rate of 3600 rpm with slope and detection limit (S/N = 3) of 0.11 microA/microM and 33 nM, respectively. Interference by direct electrochemical oxidation of easily oxidizable substances can be prevented as a result of the low detecting potential of the working system. A glucose biosensor was further constructed by coating with glucose oxidase and Tosflex on the CPE/CFe*-RP (denoted as CPE/CFe*-RP/GOx/Ts). The proposed CPE/CFe*-RP/GOx/Ts with a two-layer configuration, that is, enzyme and protecting layers, exhibits good operational performance in terms of response time, linearity, detection limit, and lifetime.
authors.affiliation: Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan. jmzen@dragon.nchu.edu.tw
authors.forename: Jyh-Myng
authors.initials: JM
authors.lastname: Zen
authors.forename: Annamalai
authors.initials: A
authors.lastname: Senthil Kumar
authors.forename: Ching-Rue
authors.initials: CR
authors.lastname: Chung
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D002244:Carbon; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007220:Industrial Waste; D009942:Organometallic Compounds / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D007220:Industrial Waste; D009942:Organometallic Compounds; D002244:Carbon; D012428:Ruthenium; D005949:Glucose Oxidase; D005947:Glucose