title: IFN-gamma-producing gamma delta T cells help control murine West Nile virus infection.
abstract: West Nile (WN) virus causes fatal meningoencephalitis in laboratory mice, thereby partially mimicking human disease. Using this model, we have demonstrated that mice deficient in gammadelta T cells are more susceptible to WN virus infection. TCRdelta(-/-) mice have elevated viral loads and greater dissemination of the pathogen to the CNS. In wild-type mice, gammadelta T cells expanded significantly during WN virus infection, produced IFN-gamma in ex vivo assays, and enhanced perforin expression by splenic T cells. Adoptive transfer of gammadelta T cells to TCRdelta(-/-) mice reduced the susceptibility of these mice to WN virus, and this effect was primarily due to IFN-gamma-producing gammadelta T cells. These data demonstrate a distinct role for gammadelta T cells in the control of and prevention of mortality from murine WN virus infection.
authors.affiliation: Department of Internal Medicine, Section of Rheumatology, Yale University School of Medicine, 300 Cedar Street, New Haven, CT 06520, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.forename: Eileen
authors.initials: E
authors.lastname: Scully
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Yin
authors.forename: Jung H
authors.initials: JH
authors.lastname: Kim
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Mark
authors.initials: M
authors.lastname: Mamula
authors.forename: John F
authors.initials: JF
authors.lastname: Anderson
authors.forename: Joe
authors.initials: J
authors.lastname: Craft
authors.forename: Erol
authors.initials: E
authors.lastname: Fikrig
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001769:Blood / Q000276:immunology / Q000821:virology; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D018792:Encephalitis, Viral / Q000235:genetics / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D005260:Female; D019674:Genes, T-Cell Receptor beta / Q000235:genetics; D019676:Genes, T-Cell Receptor delta / Q000235:genetics; D020022:Genetic Predisposition to Disease; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000502:physiology; D008221:Lymphoid Tissue / Q000276:immunology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000172:deficiency / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D012720:Severity of Illness Index; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D019562:Viral Load; D014901:West Nile Fever / Q000235:genetics / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014902:West Nile virus / Q000276:immunology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma