Multicenter evaluation of the fully automated bactec MGIT 960 system and three molecular methods for the isolation and the identification of mycobacteria from clinical specimens.
A combination of Bactec MGIT 960 system, a PCR-based assay and a PCR-Restriction Analysis procedure (PRA) was assessed for the detection and identification of mycobacteria from clinical samples. The MGIT recovered 243 from 266 mycobacterial isolates. The PCR-based assay correctly identified all (234) Mycobacterium tuberculosis isolates, while 35 nontuberculous mycobacterial isolates were identified by PRA.
Kontos
F
F
Department of Microbiology, Medical School, University Hospital of Larissa, Papakyriazi 22, Larissa, Greece.
Petinaki
E
E
Nicolaou
S
S
Gitti
Z
Z
Anagnostou
S
S
Maniati
M
M
Costopoulos
C
C
Tselentis
I
I
Maniatis
A N
AN
D001331:Automation; D015373:Bacterial Typing Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic