title: Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC.
abstract: The protective effect of Acanthopanax senticosus (AS) against ethanol (EtOH)-induced apoptosis of the human neuroblastoma cell line SK-N-MC was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 assay. It was shown that cells treated with EtOH exhibit classical apoptotic features, while cells pre-treated with Acanthopanax senticosus prior to EtOH exposure showed decreased occurrence of apoptotic features. In addition, Acanthopanax senticosus pre-treatment was shown to inhibit EtOH-induced increase in caspase-3 mRNA expression and activity. These results suggest that Acanthopanax senticosus may exert a protective effect against EtOH-induced apoptosis of human neuroblastoma cells.
authors.affiliation: Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Youn-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Seo
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Kyu-Yeol
authors.initials: KY
authors.lastname: Kim
authors.forename: Choong-Yeol
authors.initials: CY
authors.lastname: Lee
authors.forename: Kyeong-Mi
authors.initials: KM
authors.lastname: Kim
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D028001:Eleutherococcus; D000431:Ethanol; D005434:Flow Cytometry; D006801:Humans; D009447:Neuroblastoma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D000431:Ethanol; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; C022616:thiazolyl blue