Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.
OBJECTIVE
To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.


METHODS
Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.


RESULTS
Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.


CONCLUSIONS
siRNA may be effective in inhibiting SARS-associated coronavirus replication.
Zhang
Renli
R
Sun Yat-Sen University, Guangzhou 510089, China.
Guo
Zhongmin
Z
Lu
Jiahai
J
Meng
Jinxiu
J
Zhou
Canquan
C
Zhan
Ximei
X
Huang
Bing
B
Yu
Xinbing
X
Huang
Min
M
Pan
Xinghua
X
Ling
Wenhua
W
Chen
Xigu
X
Wan
Zhuoyue
Z
Zheng
Huanying
H
Yan
Xinge
X
Wang
Yifei
Y
Ran
Yanchao
Y
Liu
Xinjian
X
Ma
Junxin
J
Wang
Chengyu
C
Zhang
Biliang
B
D000818:Animals; D002522:Chlorocebus aethiops; D034741:RNA, Small Interfering / Q000494:pharmacology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects*; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
D034741:RNA, Small Interfering