title: Matrix-dependent plasticity of the malignant phenotype of bladder cancer cells.
abstract: The purpose of this study was to investigate the effect of cancer- and normal basement membrane-derived extracellular matrix to modulate the phenotype of bladder cancer cell lines. Five lines, varying in malignancy from papilloma to highly undifferentiated and invasive and immortalized human urothelial cells, were grown on two extracellular matrix preparations, Matrigel and SISgel. Matrigel represents matrix remodeled by malignancy while SISgel, obtained from small intestine submucosa (SIS), represents the normal matrix supporting differentiated cell growth. On Matrigel, regardless of the content of growth factors, the invasive lines displayed an invasive phenotype, while the low grade lines grew as papillary structures. In contrast, when the same cells were grown on SISgel, they grew as a layer of cells one to 5 cells thick, failed to invade, and expressed cell-surface E-cadherin. Unlike breast cancer cells, neutralization of beta 1, beta 4 and alpha 6 integrins altered cell-cell and cell-matrix adhesiveness but did not alter the phenotype. When invasive cells were grown on mixtures of SISgel and Matrigel, the phenotype changed gradually, not abruptly, indicating that factors within the gel reversibly alter the phenotypic expression of invasion. In summary, the phenotype of bladder cancer cells growing in tissue-like 3-dimensional culture is highly plastic, and malignant properties such as invasion and papillary growth can be suppressed by the matrix.
authors.affiliation: Departments of Urology, Biochemistry and Molecular Biology, Physiology (REH), University of Oklahoma Health Sciences Center, 140 BMSB, 940 S.L. Young Blvd, Oklahoma City, OK 73104, USA. robert-hurst@ouhsc.edu
authors.forename: Robert E
authors.initials: RE
authors.lastname: Hurst
authors.forename: Kimberly D
authors.initials: KD
authors.lastname: Kyker
authors.forename: Rebecca B
authors.initials: RB
authors.lastname: Bonner
authors.forename: Ron D
authors.initials: RD
authors.lastname: Bowditch
authors.forename: George P
authors.initials: GP
authors.lastname: Hemstreet
mesh_terms: D015820:Cadherins / Q000096:biosynthesis; D002455:Cell Division / Q000502:physiology; D003094:Collagen; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000502:physiology; D007797:Laminin; D010641:Phenotype; D011509:Proteoglycans; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D015820:Cadherins; D004338:Drug Combinations; D016023:Integrins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen