title: Expression of the TSLC1 adhesion molecule in pulmonary epithelium and its down-regulation in pulmonary adenocarcinoma other than bronchioloalveolar carcinoma.
abstract: TSLC1 (tumor suppressor in lung cancer-1) is an adhesion molecule of the Ig superfamily that binds homophilically and mediates cell-cell interactions. Originally, TSLC1 was cloned as a candidate tumor suppressor from the genomic region that frequently exhibits loss of heterogeneity in human non-small-cell lung cancer (NSCLC). However, there have been no studies on TSLC1 expression in normal lungs or NSCLC. Here we show that pulmonary epithelial cells express TSLC1 and its expression levels are often decreased or lost in primary pulmonary adenocarcinoma, a major histologic type of NSCLC. Immunohistochemistry revealed that TSLC1 was localized at cell-cell boundaries of all columnar epithelial cells in mouse embryonic lungs of 10.5 and 13 days postcoitus. Similar staining patterns were observed in bronchiolar and alveolar epithelial cells of adult human lungs, suggesting a physiologic role for TSLC1 in interactions of these cells. Next we performed Western blot analyses of TSLC1 in 47 primary pulmonary adenocarcinomas and judged each tumor as either decreased or nondecreased by comparing TSLC1 expression levels of the tumor with the levels of normal lungs. The expression profiles had a significant relation to histologic subtypes but not to other clinicopathologic parameters. Sixteen bronchioloalveolar carcinomas (BACs) were all judged nondecreased, while 19 of 31 (63%) adenocarcinomas other than BAC were judged decreased (p < 0.0001). Immunohistochemistry of tumors judged nondecreased revealed that not only BAC cells but also tumor cells in lepidic growth components of adenocarcinomas other than BAC expressed TSLC1 on their lateral plasma membranes. These tumor cells are considered less invasive because they proliferate in a lepidic growth pattern along alveolar walls. Thus, the present results not only support the hypothesis that TSLC1 is a tumor suppressor of NSCLC but also suggest that preserved integrity of TSLC1 may contribute to less invasive phenotypes of lepidic growth tumor cells.
authors.affiliation: Department of Pathology, Osaka University Medical School, Suita, Osaka, Japan. aito@patho.med.osaka-u.ac.jp
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ito
authors.forename: Morihito
authors.initials: M
authors.lastname: Okada
authors.forename: Kazuya
authors.initials: K
authors.lastname: Uchino
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Yu-Ichiro
authors.initials: Y
authors.lastname: Koma
authors.forename: Shoichi
authors.initials: S
authors.lastname: Iseki
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tsubota
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Okita
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kitamura
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000145:classification / Q000378:metabolism* / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015153:Blotting, Western; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D015536:Down-Regulation; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007136:Immunoglobulins; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D008175:Lung Neoplasms / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008875:Middle Aged; D009367:Neoplasm Staging; D038081:Organogenesis; D011506:Proteins / Q000378:metabolism*; D020545:Respiratory Mucosa; D025521:Tumor Suppressor Proteins
chemical_list: C403514:CADM1 protein, human; C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; D011506:Proteins; D025521:Tumor Suppressor Proteins