title: One-pot synthesis of L-Fructose using coupled multienzyme systems based on rhamnulose-1-phosphate aldolase.
abstract: Two methods have been developed for the highly efficient enzymatic synthesis of L-fructose: one is based on rhamnulose-1-phosphate aldolase and acid phosphatase using racemic glyceraldehyde and dihydroxyacetone phosphate as substrates; the other is to generate enantiomerically pure L-glyceraldehyde in situ from glycerol for the aldol reaction, using galactose oxidase catalyzed oxidation of glycerol in the presence of catalase. Using this four-enzyme system, enantiomerically pure L-fructose was obtained. Using the more expensive dihydroxyacetone phosphate, the yield was 55% after purification.
authors.affiliation: Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Dirk
authors.initials: D
authors.lastname: Franke
authors.forename: Timothy
authors.initials: T
authors.lastname: Machajewski
authors.forename: Che-Chang
authors.initials: CC
authors.lastname: Hsu
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D000135:Acid Phosphatase; D000446:Aldehyde-Lyases; D002374:Catalase; D005632:Fructose / Q000138:chemical synthesis* / Q000737:chemistry*; D007202:Indicators and Reagents; D010766:Phosphorylation; D013237:Stereoisomerism
chemical_list: D007202:Indicators and Reagents; D005632:Fructose; D002374:Catalase; D000135:Acid Phosphatase; D000446:Aldehyde-Lyases; C022526:rhamnulose-1-phosphate adolase