[TLC-FT-SERS study on ingredients of Isrhynchophylline].
A new method for analysing the ingredients of Isrhynchophylline in Uncaria Rhynchophylla Jacks by thin layer chromatography (TLC) and the surface-enhanced Raman spectroscopy (SERS) is reported in this paper. The results show that the characteristic spectra bands of Isrhynchophylline situated at the thin layer with the amount of sample about 2.5 micrograms were obtained. The difference between SERS and solid spectra was found. Great enhancement of the 1,615 cm-1 spectral band was abstained. Molecule was absorbed in surface silver sol by pi electrons in phenyl and by pair of electrons in N together. An absorption model of Isrhynchophylline and silver sol was proposed. This method can be used to analyse the chemical ingredients with high sensitivity.
Wang
Yuan
Y
Chemistry Department, Capital Normol University, Beijing 100037, China.
Wang
Song-ying
SY
Zhao
Yi-xue
YX
Ren
Gui-fen
GF
Zi
Feng-lan
FL
D002317:Cardiovascular Agents / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D013141:Spiro Compounds / Q000032:analysis / Q000737:chemistry*; D032070:Uncaria / Q000737:chemistry*
D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; C482693:Isrhynchophylline; D013141:Spiro Compounds