[Preliminary identification of up-regulated genes after the interaction of human peripheral blood mononuclear cell and porcine endothelial cell].
OBJECTIVE
To investigate the change of gene expression after the interactions of human peripheral blood mononuclear cell (PBMC) and porcine endothelial cell.


METHODS
Human PBMC were co-cultured with porcine vascular endothelial cell line PIEC for 18 h. The mixed un-co-cultured PBMC and PIEC were used as controls. The cDNAs of both groups were prepared and subtracted by suppression subtractive hybridization (SSH). The up-regulated cDNAs were cloned into pGEM-T Easy vector to be sequenced. Nucleic acid homology searches were performed using the BLAST program.


RESULTS
A subtracted cDNA library was obtained through SSH and cloning, which included about 300 clones corresponding to up-regulated genes. We randomly selected 24 of these clones for sequencing tests and analysis of homology in the GenBank. Four of the 24 clones were part of known human genes, 8 were known porcines expressed sequence tag (EST), and 9 held similar homology with known human genes. Three clones were not matched.


CONCLUSIONS
Many genes are up-regulated after the interaction of human PBMC and porcine endothelial cell.
Hu
Weimin
W
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Cheng
Jingqiu
J
Li
Shengfu
S
Lu
Xiaofeng
X
Wan
Lin
L
Li
Youping
Y
D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018920:Coculture Techniques; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology*; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism*; D016324:Sequence Tagged Sites; D013552:Swine; D014183:Transplantation, Heterologous; D015854:Up-Regulation
D018076:DNA, Complementary