Polymerase chain reaction amplification of samples from Japanese monkeys using primers for human short tandem repeat loci.
We examined the species specificity of six commercially available human short tandem repeat systems (for CSF1PO, TPOX, TH01, F13A01, FESFPS and vWA) using six samples obtained from the Japanese monkey Macaca fuscata. Macaque genes were amplified with human TPOX and F13A01 primers. During electrophoresis, macaque polymerase chain reaction (PCR) products amplified with the TPOX primer migrated more slowly than human ones, and migrated close to human CSF1PO bands. Macaque PCR products amplified with the F13A01 primer also migrated more slowly than the human equivalent. The six macaque samples were classified into three types by the TPOX primer and into six types by the F13A01 primer. These results suggest that the primers for human TPOX and F13A01 loci can be used to distinguish between human and Japanese macaque samples.
Ago
Kazutoshi
K
Department of Legal Medicine, Faculty of Medicine, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima 890-8520, Japan. ago@m.kufm.kagoshima-u.ac.jp
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Mihoko
M
Orihara
Yoshiyuki
Y
Nakagawa
Shiro
S
Ogata
Mamoru
M
D000818:Animals; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000032:analysis*; D004586:Electrophoresis; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA