title: Crystallization and preliminary X-ray diffraction studies of a toxic phospholipase A2 from the venom of Vipera ammodytes meridionalis complexed to a synthetic inhibitor.
abstract: A toxic phospholipase A(2) (PLA(2)) is isolated from the neurotoxic complex Vipoxin, the major lethal component of the venom of Vipera ammodytes meridionalis. The enzyme is complexed to the synthetic inhibitor elaidoylamide and crystallized. The crystals belong to the space group P2(1)2(1)2(1), with unit cell dimensions a=46.57 A, b=82.68 A, c=119.47 A and beta=90 degrees. Initial diffraction data to 3.3 A resolution are collected.
authors.affiliation: Universitätsklinikum Hamburg-Eppendorf, Zentrum für Experimentelle Medizin, Institut für Biochemie und Molekularbiologie I, c/o DESY, Notkestrasse 85, Geb. 22a, 22603 Hamburg, Germany.
authors.forename: Dessislava Nikolova
authors.initials: DN
authors.lastname: Georgieva
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rypniewski
authors.forename: Markus
authors.initials: M
authors.lastname: Perbandt
authors.forename: Mahendra
authors.initials: M
authors.lastname: Jain
authors.forename: Nicolay
authors.initials: N
authors.lastname: Genov
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
mesh_terms: D000577:Amides / Q000378:metabolism; D000818:Animals; D018360:Crystallography, X-Ray; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D054467:Phospholipases A2; D014757:Viper Venoms / Q000737:chemistry* / Q000235:genetics; D017819:Viperidae / Q000235:genetics*
chemical_list: D000577:Amides; D014757:Viper Venoms; C033134:vipoxin; D010741:Phospholipases A; D054467:Phospholipases A2