title: [Quantitative detection of hepatitis B virus by fluorescence quantitative PCR and its clinical value].
abstract: OBJECTIVE
  To study the relationship between the serum contents of HBV DNA copies and the HBV serum index of immunology and the degree of hepatocyte injury in patients with HBV hepatitis.
  
  
  METHODS
  The contents of HBV DNA copies were detected by fluorescence quantitative PCR (FQ-PCR), and the HBV serum index of immunology by ELISA.
  
  
  RESULTS
  The contents of HBV DNA copies had a significant difference between HBeAg (+)/anti-HBe (-) group (42 patients) and HBeAg (-)/anti-HBe (+) group (57 patients) or HBeAg (-)/anti-HBe (-) group(11 patients) (P < 0.05), while there was no significant difference between HBeAg (-)/anti-HBe (+) group and HBeAg (-)/anti-HBe (-) group(P > 0.05). The contents of HBV DNA copies had no correlation with the severe degree of hepatocyte injury(P > 0.05).
  
  
  CONCLUSIONS
  Anti-HBe is not the terminal sign of HBV replication. The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV DNA copies.
authors.affiliation: Department of Infectious Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Xian-shi
authors.initials: XS
authors.lastname: Su
authors.forename: Xuan-he
authors.initials: XH
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005453:Fluorescence; D006509:Hepatitis B / Q000821:virology*; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens