Short tandem repeat typing in exon 1 of the androgen receptor gene.
Exon 1 of the androgen receptor (AR) gene on the X chromosome contains a polymorphic CAG trinucleotide short tandem repeat. We describe here the rapid and reliable method of typing CAG repeats using electrophoresis, with denaturing polyacrylamide gel and an allelic ladder marker. Twenty-one alleles (the repeat number ranges from 13 to 35) were found using CAG repeat typing in normal Japanese individuals (83 males and 82 females). The allelic diversity (h) calculated was h=0.889, illustrating that CAG repeats at the AR locus is a highly polymorphic system.
Fujii
Kenji
K
Department of Legal Medicine, Ehime University School of Medicine, Ehime 791-0295, Japan. nishimuk@m.ehime-u.ac.jp
Okiura
Tatsuyuki
T
Nishimura
Koji
K
Orimoto
Chitoshi
C
Nishimukai
Hiroaki
H
D041321:Chromosomes, Human, X; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D011944:Receptors, Androgen