Protective activity of Lentinan in experimental tuberculosis.
Protective effects of Lentinan (Ajinomoto, Japan) against Mycobacterium tuberculosis infection were studied by in vitro and in vivo mouse models. The effectiveness of Lentinan administrated intraperitoneally (i.p.) before infection at a dose of 1 mg/kg three times at 2-day intervals was monitored in vivo by several parameters (body temperature; spleen weight; CFU counts of M. tuberculosis in spleen, liver and lung; and histomorphological observations). Peritoneal macrophages obtained from animals treated with Lentinan were greatly stimulated, as assayed by establishing their number, acid phosphatase activity, H2O2 production and killing ability against M. tuberculosis in vitro. The in vivo model demonstrated that administration of Lentinan before infection can mobilize host defense potential and reduce mycobacterial infection.
Markova
Nadya
N
Department of Pathogenic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26 G. Bonchev St., 1113 Sofia, Bulgaria. semahead@geobiz.net
Kussovski
Vesselin
V
Drandarska
Ivanka
I
Nikolaeva
Sascha
S
Georgieva
Neli
N
Radoucheva
Tatyana
T
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D007274:Injections, Intraperitoneal; D007912:Lentinan / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000302:isolation & purification; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D014376:Tuberculosis / Q000517:prevention & control*
D000276:Adjuvants, Immunologic; D007912:Lentinan