title: Genes expressed by the kidney, but not by bone marrow-derived cells, underlie the genetic predisposition to progressive glomerulosclerosis after mesangial injury.
abstract: Progressive renal failure is accompanied by uncontrolled accumulation of extracellular matrix in glomeruli and tubulointerstitium, eventually resulting in glomerulosclerosis. Although glomerulosclerosis occurs secondary to various renal diseases, the fact that not all patients develop progressive glomerulosclerosis suggests that genetic factors may underlie the tendency to progress, or not to progress. Identified were two Lewis rat substrains with small genetic differences but with considerable difference in resolution of glomerulonephritis after anti-Thy-1 administration. In the Lewis/Møllegard rat strain, anti-Thy-1 glomerulonephritis spontaneously resolves within 4 wk. In contrast, Lewis/Maastricht rats develop progressive glomerulosclerosis after induction of this disease. The involvement of bone marrow-derived cells and kidney cells in the development of glomerulosclerosis was determined. In the first study, exchange of bone marrow between these substrains did not affect the course of anti-Thy-1 nephritis. Lewis/Møllegard rats recovered rapidly, but Lewis/Maastricht rats showed progressive disease regardless of the genotype of the bone marrow they received. In the second study, kidneys were exchanged between the substrains. After transplantation, anti-Thy-1 nephritis was induced and glomerular damage assessed at day 21. Severe damage was observed in Lewis/Maastricht glomeruli independent of whether the kidney had been transplanted or not. Similarly, Lewis/Møllegard glomeruli, whether transplanted or not, revealed no residual histopathologic abnormalities. The inherited differences between the two substrains with regard to their insusceptibility to develop progressive glomerulosclerosis after mesangial injury are governed by genes expressed by the kidney, but not by bone marrow-derived cells.
authors.affiliation: Departments of Pathology and Nephrology, Leiden University Medical Center, Leiden, The Netherlands. j.a.aben@lumc.nl
authors.forename: Joris A
authors.initials: JA
authors.lastname: Aben
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Hoogervorst
authors.forename: Leendert C
authors.initials: LC
authors.lastname: Paul
authors.forename: Maria C
authors.initials: MC
authors.lastname: Borrias
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Noble
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Border
authors.forename: Jan A
authors.initials: JA
authors.lastname: Bruijn
authors.forename: Emile
authors.initials: E
authors.lastname: de Heer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D005920:Glomerular Mesangium / Q000473:pathology / Q000503:physiopathology*; D005923:Glomerulosclerosis, Focal Segmental / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D007518:Isoantibodies; D007668:Kidney / Q000503:physiopathology*; D016030:Kidney Transplantation; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C035678:anti-Thy antibody