[Correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria gonorrhoeae].
OBJECTIVE
To investigate the correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria Gonorrhoeae.


METHODS
The gene fragments of quinolone resistance-determining region (QRDR) in GyrA and ParC genes in 20 Neisseria gonorrhoeae strains clinically isolated in Wuxi, China, were sequenced, and the susceptibility of the 20 strains to quinolone was examined by agar diffusion method.


RESULTS
The mutations at the Asp95 point in GyrA gene were found in 20 strains. Of the 19 stains examined, 16 had mutations at the 86, 87, 88, 91 points in ParC genes.


CONCLUSIONS
The mutations of Asp95 in GyrA gene and Asp86, Ser87, Ser88, Glu91 in ParC gene contribute to quinolone resistance in Neisseria Gonorrhoeae.
Xie
Ping
P
Central Laboratory, No. 1 People's Hospital of Wuxi, Wuxi, Jiangsu 214002, China.
Mi
Zu-Huang
ZH
Li
Qin
Q
Zhang
Ji
J
Xiao
Chen-Yue
CY
D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D015363:Quinolones / Q000494:pharmacology*
D015363:Quinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase