Equilibrium water exchange between the intra- and extracellular spaces of mammalian brain.
This report describes the measurement of water preexchange lifetimes and intra/extracellular content in intact, functioning mammalian brain. Intra- and extracellular water magnetic resonance (MR) signals from rat brain in vivo were quantitatively resolved in the longitudinal relaxation domain following administration of an MR relaxation agent into the extracellular space. The estimated intracellular water content fraction was 81% +/- 8%, and the intra- to extracellular exchange rate constant was 1.81 +/- 0.89 s(-1) (mean +/- SD, N = 9), corresponding to an intracellular water preexchange lifetime of approximately 550 ms. These results provide a temporal framework for anticipating the water exchange regime (fast, intermediate, or slow) underlying a variety of compartment-sensitive measurements. The method also supplies a means by which to evaluate membrane water permeability and intra/extracellular water content serially in intact tissue. The data are obtained in an imaging mode that permits detection of regional variations in these parameters.
Quirk
James D
JD
Department of Chemistry, Washington University, St. Louis, Missouri 63110, USA.
Bretthorst
G Larry
GL
Duong
Timothy Q
TQ
Snyder
Avi Z
AZ
Springer
Charles S
CS
Ackerman
Joseph J H
JJ
Neil
Jeffrey J
JJ
D000818:Animals; D001499:Bayes Theorem; D001834:Body Water / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003287:Contrast Media / Q000008:administration & dosage; D017352:Echo-Planar Imaging / Q000379:methods*; D005110:Extracellular Space / Q000378:metabolism; D019786:Gadolinium DTPA / Q000008:administration & dosage; D042541:Intracellular Space / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013238:Stereotaxic Techniques
D003287:Contrast Media; D019786:Gadolinium DTPA