title: [Using two-dimensional gel electrophoresis to analyze the regeneration microenvironment of facial nerve and its response to acupuncture].
abstract: OBJECTIVE
  To investigate the distribution of proteins in the regeneration microenvironment of facial nerve and its response to acupuncture.
  
  
  METHODS
  We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two-dimensional gel electrophoresis.
  
  
  RESULTS
  We found that most proteins in the regeneration fluid were under relative molecular mass 80 x 10(3) and in the range of pI 4-7. An average of 800 +/- 53 protein spots was found in the control group compared to the 850 +/- 78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups.
  
  
  CONCLUSIONS
  There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve.
authors.affiliation: Otonasolaryngeal Department, West China Hospital, Sichuan Universty, Chengdu 610041, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Cairong
authors.initials: C
authors.lastname: Yang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Lu
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Liang
authors.forename: Bingqing
authors.initials: B
authors.lastname: Gao
mesh_terms: D026881:Acupuncture; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005154:Facial Nerve / Q000502:physiology / Q000503:physiopathology*; D020220:Facial Nerve Injuries / Q000503:physiopathology; D005260:Female; D008297:Male; D009416:Nerve Regeneration; D011506:Proteins / Q000032:analysis*; D011817:Rabbits
chemical_list: D011506:Proteins