[Expression and significance of transforming growth factor-beta1, matrix metalloproteinase-2 and its inhibitor in lens epithelial cells of diabetic cataract].
OBJECTIVE
To investigate the expression and significance of transforming growth factor-beta(1) (TGF-beta(1)), matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in human Lens epithelial cells (LEC) of diabetic cataract.


METHODS
Immunohistochemical staining was used to detect the expression of TGF-beta(1), MMP-2 and TIMP-2 in the diabetic LEC (23 cases) and the normal LEC (7 cases).


RESULTS
There was significant difference of expression of MMP-2 and TGF-beta(1) between diabetic LEC and normal LEC (P < 0.01). But there was no statistic significance for TIMP-2 expression (P > 0.05). There was correlation between the expression of TGF-beta(1) and MMP-2.


CONCLUSIONS
The imbalance induced by TGF-beta(1) between the expression of MMP-2 and TIMP-2 may play a critical role in the pathological fibrosis of anterior and posterior subcapsular during the development of diabetic cataract.
Xu
Guo-xing
GX
Fujian institute of ophthalmology, The first affiliated hospital of Fujian Medical University, Fuzhou 350005, China. zjfmuxgx@pub5.fz.fj.cn
Hu
Jian-zhang
JZ
Zheng
Wei-dong
WD
Zhang
Sheng
S
Wang
Ting-ting
TT
D000328:Adult; D002386:Cataract / Q000378:metabolism*; D048909:Diabetes Complications / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2