Synthesis and photoinduced DNA cleaving activity of hairpin polyamide-chlorobenzenesulfonyl conjugates.
A hairpin polyamide-chlorobenzenesulfonyl conjugate was synthesized in solution by a haloform reaction and the dicyclohexylcarbodiimide/1-hydroxybenzotriazole (DCC/HOBT) coupling reaction. Its ability to cleave DNA was investigated with supercoiled DNA (pBluescript SK), and the DNA cleaving efficiency of chlorobenzenesulfonyl group was enhanced by the hairpin polyamide under UV irradiation (365 nm).
Tang
Fei Li
FL
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Education Ministry, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, People's Republic of China.
Yuan
Gu
G
Wang
Jin
J
D000577:Amides / Q000138:chemical synthesis / Q000494:pharmacology*; D004247:DNA / Q000138:chemical synthesis / Q000187:drug effects / Q000528:radiation effects*; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects; D008968:Molecular Conformation; D013449:Sulfonamides / Q000138:chemical synthesis / Q000494:pharmacology*; D014466:Ultraviolet Rays; D000096926:Benzenesulfonamides
D000577:Amides; D004247:DNA; D004278:DNA, Superhelical; D013449:Sulfonamides; C479130:N-PyPyPygammaImImImbetaCO-4-aza-4-methylheptyl 4-chlorobenzenesulfonamide; D000096926:Benzenesulfonamides