title: Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation.
abstract: When paternally transmitted, two independent ENU-induced mutations showed reduced whole body wet weight soon after birth. The mutations were mapped to Chromosome 9 (Chr 9) between the markers D9Mit208 and D9Mit215. Their map position and imprinted status suggested that they might alter RAS protein-specific guanine nucleotide releasing factor 1 expression. Both mutations introduced premature chain termination codons into the coding sequence of Rasgrf1, and no Ras-GRF1 protein was detected in the brain. The GENA53 line had a C to T transition at nucleotide 2137, and the line GENA37 had a T to A transversion at nucleotide 3552 of the cDNA sequence. Mutant mice had near normal body weight at birth, but their weight started to lag behind that of wild-type littermates during the first week, and they were about 15% lighter as adults.
authors.affiliation: Zoology Department, South Parks Road, Oxford, OX1 3PS, UK.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Clapcott
authors.forename: Josephine
authors.initials: J
authors.lastname: Peters
authors.forename: Paul C
authors.initials: PC
authors.lastname: Orban
authors.forename: Riccardo
authors.initials: R
authors.lastname: Brambilla
authors.forename: Chris F
authors.initials: CF
authors.lastname: Graham
mesh_terms: D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002874:Chromosome Mapping; D018388:Codon, Terminator / Q000235:genetics; D003433:Crosses, Genetic; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D005038:Ethylnitrosourea / Q000633:toxicity; D019204:GTP-Binding Proteins / Q000235:genetics*; D020690:GTPase-Activating Proteins; D008040:Genetic Linkage / Q000235:genetics*; D006130:Growth Disorders / Q000235:genetics*; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D009153:Mutagens / Q000633:toxicity; D012097:Repressor Proteins; D017422:Sequence Analysis, DNA; D020666:ras-GRF1
chemical_list: C513041:Arhgap35 protein, mouse; D018388:Codon, Terminator; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020690:GTPase-Activating Proteins; D009153:Mutagens; C513037:Rasgrf1 protein, mouse; D012097:Repressor Proteins; D020666:ras-GRF1; D019204:GTP-Binding Proteins; D005038:Ethylnitrosourea