Involvement of prostanoids in the inhibitory effect of endothelin-1 on progesterone production of ovarian granulosa cells.
The present study was undertaken to investigate the possible involvement of prostanoids in the inhibitory effect of endothelin-1 (ET-1) on progesterone production of ovarian granulosa cells. ET-1 (1 x 10(-7) M) decreased the basal and follicle-stimulating hormone- (FSH) stimulated progesterone production from both human and porcine granulosa cells. Indomethacin dose-dependently inhibited progesterone release, but did not alter the inhibitory effect of ET-1 (1 x 10(-7) M) on progesterone production of cultured ovarian granulosa cells. This study shows that ET-1 suppresses basal and FSH-stimulated progesterone production by ovarian granulosa cells, but this effect is not mediated by prostanoids.
Yaneva
E
E
Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria. kateyan@abv.bg
Denkova
R
R
Bourneva
V
V
Zvetkova
E
E
Baleva
K
K
Nikolov
B
B
D000818:Animals; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D011374:Progesterone / Q000096:biosynthesis*; D011453:Prostaglandins / Q000378:metabolism* / Q000494:pharmacology; D013552:Swine
D019332:Endothelin-1; D011453:Prostaglandins; D011374:Progesterone