Adsorptive stripping voltammetric determination of cefepime at the mercury electrode in human urine and cerebrospinal fluid, and differential pulse polarographic determination in serum.
The cephalosporin cefepime has been studied by adsorptive stripping voltammetric on the hanging mercury drop electrode, followed by linear sweep voltammetry (staircase). The adsorptive stripping response was evaluated with respect to preconcentration dependence and other variables. The drug is strongly adsorbed in acid media, with maximum adsorption at pH 5.8. The detection limit found was 4.8 x 10(-10) M, with 120-s preconcentration. The relative standard deviation at the 10(-7) M level was 0.93%. This method was applied to the determination of cefepime in human urine and cerebrospinal fluid. Differential pulse polarography has been applied to determination in human serum.
Palacios
F J Jiménez
FJ
Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, E-41012 Seville, Spain.
Mochón
M Callejón
MC
Sánchez
J C Jiménez
JC
Carranza
J Herrera
JH
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D000077723:Cefepime; D002511:Cephalosporins / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006801:Humans; D008628:Mercury; D011048:Polarography / Q000379:methods; D044967:Serum
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000077723:Cefepime; D008628:Mercury