Trade-off between synthesis of salicylates and growth of micropropagated Salix pentandra.
We studied the relationship between biosynthesis of salicylates, the main chemical defenses in willow and growth of Salix pentandra by cultivating plants in the presence of 2-aminoindan-2-phosphonic acid (AIP), a powerful inhibitor of phenylalanine ammonia-lyase (PAL: EC 4.3.1.5.). AIP inhibited efficiently, though not totally, the endogenous synthesis of salicylates. This inhibition markedly increased plant growth. Exogenous application of the precursors of salicylates, benzoic acid (BA), salicylic acid (SA), and helicin, increased the levels of several individual salicylates, but decreased the growth of plants cultivated in the presence of AIP. These results suggest a trade-off between plant growth and the synthesis of salicylates in S. pentandra. Phenylalanine, which accumulated in response to inhibitor treatment, but was decreased by precursor treatments, may be a common and limiting precursor for both plant growth and salicylate synthesis. The biosynthesis of salicin is suggested to proceed mainly via benzoyl-glucose, an intermediate in the synthesis of salicylic acid. Salicin is the most obvious precursor of more substituted salicylates, salicortin, acetylsalicortin, and tremulacin. In addition, we found that the salicylate pools of mature plant parts of S. pentandra were not subject to turnover, implying that the maintenance of salicylates does not demand high resources of plants, although their initial construction is costly.
Ruuhola
Teija
T
Department of Biology, University of Joensuu, P.O. Box 111 FIN-80101 Joensuu, Finland. teiruu@utu.fi
Julkunen-Titto
Riitta
R
D000222:Adaptation, Physiological; D000818:Animals; D005247:Feeding Behavior; D007189:Indans; D007313:Insecta; D063065:Organophosphonates / Q000494:pharmacology; D010649:Phenylalanine / Q000032:analysis / Q000096:biosynthesis; D010945:Plants, Edible; D012459:Salicylates / Q000032:analysis*; D032108:Salix / Q000737:chemistry / Q000254:growth & development*
C411146:2-aminoindan-2-phosphonic acid; D007189:Indans; D063065:Organophosphonates; D012459:Salicylates; D010649:Phenylalanine