title: Transcription of TIM9, a new factor required for the petite-positive phenotype of Saccharomyces cerevisiae, is defective in spt7 mutants.
abstract: TIM9 has been identified as an additional novel gene required for the petite-positive phenotype in Saccharomyces cerevisiae. tim9-1 was obtained through a screen for respiratory-deficient strains that are unable to survive in the absence of mitochondrial DNA. A point mutation found in the tim9-1 coding region converts codon 71 from Gly to Arg. Examination of genes encoding other Tim components indicated that the temperature-conditional alleles of essential genes for the viability of S. cerevisiae, TIM9, TIM10 and TIM12, are required for petite survival, while deletion of TIM8 and TIM13 has no notable effect on petite cell viability. Northern hybridization results suggested that the Spt7 transcription factor is strictly involved in transcription of TIM9 and that the synergistic lethality of tim9-1/spt7Delta dual mutations is due to the deficiency of TIM9 transcription together with defective function of the tim9-1 protein.
authors.affiliation: Molecular Genetics and Evolution Group, Research School of Biological Sciences, The Australian National University, GPO Box 475, ACT 2601 Canberra, Australia.
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.forename: Xin Jie
authors.initials: XJ
authors.lastname: Chen
authors.forename: G Desmond
authors.initials: GD
authors.lastname: Clark-Walker
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D004252:DNA Mutational Analysis; D017931:DNA Primers; D026901:Membrane Transport Proteins / Q000235:genetics*; D008928:Mitochondria / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D020125:Mutation, Missense / Q000235:genetics; D010641:Phenotype; D017354:Point Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics* / Q000502:physiology
chemical_list: D017931:DNA Primers; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; C093107:SPT7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C519190:Tim8 protein, S cerevisiae; C116366:Tim9 protein, S cerevisiae; D014157:Transcription Factors