[Determination of ciprofloxacin in urine using terbium ion as fluorescent probe].
Factors effecting the fluorescence characteristics of the reaction CPFX-Tb were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Tb. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 x 10(-8)-1.0 x 10(-6) mol.L-1. The regression equation is F = 1.57 + 4.87 x 10(8) c, r = 0.9999 with the detection limit of 5.0 x 10(-9) mol.L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe.
Wang
L
L
Department of Pharmacy, Shandong Medical University, Jinan 250012, China.
Sang
L H
LH
Wu
Y L
YL
Cheng
X M
XM
Su
Z D
ZD
Yu
A H
AH
D000890:Anti-Infective Agents / Q000652:urine*; D002614:Chelating Agents; D002939:Ciprofloxacin / Q000652:urine*; D005456:Fluorescent Dyes; D006801:Humans; D013050:Spectrometry, Fluorescence; D013725:Terbium
D000890:Anti-Infective Agents; D002614:Chelating Agents; D005456:Fluorescent Dyes; D013725:Terbium; D002939:Ciprofloxacin