Production of poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) with flexible 3-hydroxyhexanoate content in Aeromonas hydrophila CGMCC 0911.
Aeromonas hydrophila CGMCC 0911 isolated from lake water was found to be able to synthesize a polyhydroxyalkanoate (PHA) copolymer (PHBHHx) consisting of 3-hydroxybutyrate (HB) and 4-6 mol% 3-hydroxyhexanoate (HHx). The wild-type bacterium accumulated 49% PHBHHx containing 6 mol% HHx in terms of cell dry weight (CDW) when grown on lauric acid for 48 h. When A. hydrophila CGMCC 0911 expressed the Acyl-CoA dehydrogenase gene ( yafH) of Escherichia coli, the recombinant strain could accumulate 47% PHBHHx, while the HHx content reached 17.4 mol%. The presence of changing glucose concentration in the culture changed the HHx content both in wild type and recombinant A. hydrophila CGMCC 0911. When 5 g l(-1) glucose was added to a culture containing 5 g l(-1) lauric acid as co-substrate, 45% PHBHHx/CDW consisting of 8.8 mol% HHx was produced by wild-type A. hydrophila CGMCC 0911 compared with only 5% in the absence of glucose. When the recombinant A. hydrophila CGMCC 0911 was grown on a mixed substrate containing lauric acid and 8-10 g l(-1) glucose, the HHx content could be further increased to 35.6 mol%. When the glucose concentration exceeded 10 g l(-1), cell growth, PHA content and mole percentages of HHx in PHBHHx were significantly reduced.
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Dept of Biological Science and Biotechnology, Tsinghua University, 100084 Beijing, China.
Wu
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Chen
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D020155:3-Hydroxybutyric Acid / Q000737:chemistry / Q000378:metabolism*; D042964:Acyl-CoA Dehydrogenase / Q000235:genetics / Q000502:physiology; D016980:Aeromonas hydrophila / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D020148:Butyric Acid / Q000378:metabolism; D002208:Caproates / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D002210:Caprylates / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D007850:Lauric Acids / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D000077559:Sodium Citrate; D013395:Sucrose / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D042964:Acyl-CoA Dehydrogenase; D020148:Butyric Acid; D002208:Caproates; D002210:Caprylates; D002951:Citrates; D003470:Culture Media; D005632:Fructose; D005942:Gluconates; D005947:Glucose; D007850:Lauric Acids; D019301:Oleic Acid; D011994:Recombinant Proteins; D000077559:Sodium Citrate; D013395:Sucrose; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C045051:3-hydroxyhexanoic acid; C030358:lauric acid; C031492:octanoic acid; C030691:gluconic acid