Release of inflammatory mediators from eosinophils following a hyperosmolar stimulus.
Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction (EIB). It has been shown that an in vitro hyperosmolar stimulation of basophils and mast cells with mannitol can induce the release of histamine and leukotrienes. The aim of this study was to establish if a hyperosmolar challenge could trigger activation of eosinophils to release chemokines and lipid mediators. Peripheral blood eosinophils were isolated from seven asthmatic and six non-asthmatic subjects. Hyperosmolar stimulation of eosinophils with mannitol (0.7 M), resulted in a significant increase in LTC4 levels compared to baseline in both asthmatic (15.2+/-4.6 vs. 70.1+/-9.5; P = 0.0002) and control subjects (14.3+/-4.0 vs. 55.6+/-5.6; P = 0.0001). ECP levels did not increase significantly above baseline following mannitol stimulation in either group. This study shows that eosinophils can be activated by a hyperosmolar stimulus. Therefore it seems reasonable to suggest that eosinophils could contribute to EIB.
Moloney
E D
ED
Department of Respiratory Medicine, James Connolly Memorial Hospital, Dublin, Ireland. e.moloney@ic.ac.uk
Griffin
S
S
Burke
C M
CM
Poulter
L W
LW
O'Sullivan
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D001249:Asthma / Q000378:metabolism* / Q000473:pathology; D001798:Blood Proteins / Q000378:metabolism; D016084:Bronchoconstriction / Q000502:physiology; D004234:Diuretics, Osmotic / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism*; D015444:Exercise; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D017997:Leukotriene C4 / Q000378:metabolism; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008875:Middle Aged; D009994:Osmolar Concentration; D012260:Ribonucleases
D001798:Blood Proteins; D004234:Diuretics, Osmotic; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D017997:Leukotriene C4; D008353:Mannitol; D012260:Ribonucleases