title: Survey of Vibrio cholerae O1 and its survival over the winter in marine water of Port of Osaka.
abstract: The survey of Vibrio cholerae O1 in marine area was carried out in the Port of Osaka, Japan in 1987-2001, and 51 V. cholerae O1 strains were isolated. All strains were identified to be of El Tor biotype, Ogawa serotype and classic Ubon Kappa-phage type, and were cholera toxin (CT)-negative and CT gene-negative. In order to clarify certain ecological aspects of V. cholerae O1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-field gel electrophoresis (PFGE) with NotI and SfiI restriction enzymes. We found the indistinguishable strains by DNA analysis using PFGE with strains passed for 1 year, and also found the closely related strains with that passed for 3 and 12 years. Those results indicated that V. cholerae O1 can survive over one winter at least, and that it survives in marine water for a long time by undergoing continuous mutation.
authors.affiliation: Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyagi
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Yagi
authors.forename: M
authors.initials: M
authors.lastname: Hanafusa
authors.forename: S
authors.initials: S
authors.lastname: Imura
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Sano
mesh_terms: D000222:Adaptation, Physiological; D002771:Cholera / Q000453:epidemiology*; D002980:Climate; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D007564:Japan; D009154:Mutation; D012621:Seasons; D012703:Serotyping; D016019:Survival Analysis; D041241:Vibrio cholerae O1 / Q000235:genetics / Q000472:pathogenicity*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial