Light emitting diode irradiation protect against the amyloid beta 25-35 induced apoptosis of PC12 cell in vitro.
OBJECTIVE
Whether light emitting diode (LED) irradiation has effects on "beta-amyloid (A beta) induced apoptosis," a leading hypothesis of the cause of Alzheimer's disease (AD), or not?


METHODS
Monolayer cell cultures of PC12 were subjected to A beta or/and LED irradiation at various intensity. Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay.


RESULTS
Treatment of the cells with LED irradiation significantly diminished A beta induced apoptosis within 24 hours.


CONCLUSIONS
The LED irradiation, when utilized at power of 0.9 W/m(2) and 60 minutes has significantly diminished A beta induced apoptosis of PC12 cells.
Duan
Rui
R
Laboratory of Laser Sports Medicine, College of Sports Science, South China Normal University, Guangzhou, 510631, China.
Zhu
Ling
L
Liu
Timon Cheng-Yi
TC
Li
Yan
Y
Liu
Jiang
J
Jiao
Jianling
J
Xu
Xiaoyang
X
Yao
Libo
L
Liu
Songhao
S
D016229:Amyloid beta-Peptides / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D008027:Light; D016716:PC12 Cells; D010446:Peptide Fragments / Q000502:physiology*; D051381:Rats; D013997:Time Factors
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)