The influence of an internal electric field upon protein crystallization using the gel-acupuncture method.
In this work, the influence of an internal electric field upon the crystallization of lysozyme and thaumatin is explored using a modified design of the gel-acupuncture setup. From a crystallographic point of view, the orientation of crystals that grow preferentially over different types of electrodes inside capillary tubes is also evaluated. Finally, the crystal quality and the three-dimensional structure of these proteins grown with and without the electric field influence are analyzed by means of X-ray diffraction methods.
Mirkin
N
N
Instituto de Química, UNAM Circuito Exterior, C. U. Mexico, D. F. 04510, Mexico.
Frontana-Uribe
B A
BA
Rodríguez-Romero
A
A
Hernández-Santoyo
A
A
Moreno
A
A
D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D009113:Muramidase / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
D010940:Plant Proteins; D011506:Proteins; C003427:thaumatin protein, plant; D009113:Muramidase