Man-made mineral fiber hazardous properties assessment using transgenic rodents: example of glass fiber testing.
Transgenic BigBlue rats were exposed to CM 44 glass fibers (6.3 mg/m3) by nose only, 6 h/day for 5 days. Two endpoints were examined 1, 3, 14, 28, and 90 days following exposure: fiber biopersistence and mutations in lung DNA. The half-time of the fibers >20 microm was 12.8 days, and mutant frequencies of control and exposed rats were similar across all time points. The mutation spectra of both series were similar after 28 days of fixation time. These results showed that a glass fiber with a high clearance in the lung seems to not present any significant effect on mutagenesis on lung DNA and are in marked contrast to results for asbestos, which caused a twofold mutant frequency increase as described in a previous study.
Bottin
M C
MC
Institut National de Recherche et de Sécurité, Vandoeuvre Cedex, France.
Vigneron
J C
JC
Rousseau
R
R
Micillino
J C
JC
Eypert-Blaison
C
C
Kauffer
E
E
Martin
P
P
Binet
S
S
Rihn
B H
BH
D000818:Animals; D030801:Animals, Genetically Modified; D018893:Bronchoalveolar Lavage; D004249:DNA Damage; D005898:Glass; D006207:Half-Life; D019570:Inhalation Exposure; D008297:Male; D009152:Mutagenicity Tests; D051381:Rats / Q000235:genetics; D011916:Rats, Inbred F344; D018570:Risk Assessment
C009753:fiberglass