Amiloride inhibition of glycinergic miniature IPSCs in mechanically dissociated rat spinal neurons.
The effect of amiloride on glycinergic transmission in mechanically dissociated rat spinal dorsal horn neurons was examined with the use of whole-cell patch-clamp recording. Amiloride reversibly reduced both the frequency and amplitude of spontaneous glycinergic miniature IPSCs (mIPSCs) and its inhibitory effect on glycinergic mIPSCs persisted in either Ca2+-free or Na+-free external solutions while it disappeared in K+-free external solution. Analysis of the relationship between mIPSCs amplitude and frequency at various holding potentials shows that amiloride inhibition of glycinergic mIPSC frequency could result indirectly from its inhibition of amplitude.
Li
Yong
Y
Department of Neurobiology and Biophysics, University of Science and Technology of China, Hefei 230027, China.
Li
Yan-Fang
YF
Xu
Tian-Le
TL
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005998:Glycine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects* / Q000502:physiology; D010328:Patch Tests; D020671:Posterior Horn Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011191:Potassium Deficiency / Q000378:metabolism; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology
D000584:Amiloride; D005998:Glycine