[Development of rapid methods for quantitative analysis of proteolytic reactions].
The approaches for development of express methods for quantitative control of proteolytic reactions are discussed. Recently, these reactions have taken on special significance for revealing many important problems of theoretical and practical medicine and biology as well as for technological, pharmacological and ecological monitoring. Traditional methods can be improved both by use of immobilized enzymes and substrates, and on the basis of combination of various classic biochemical and immunological approaches. The synthesis of substrates with specified properties allows new methods to be realized for the study of the proteinase activity and kinetic characteristics of the corresponding reactions both in vitro and in vivo. An application of biosensor technology is promising trend since it allows the analysis time and cost to be saved, the direct interaction between enzymes and their inhibitors and activators to be studied in a real time scale, the quantitative measurements to be performed both in liquids and in the air. Besides, biosensor technique is well compatible with computer data processing.
Beloivan
O A
OA
Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv. beloivan@biosensor.kiev.ua
Tsvetkova
M N
MN
Bubriak
O A
OA
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D001330:Electronic Data Processing; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000639:trends*
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D010450:Endopeptidases