title: Essential role of ATP synthesized by creatine kinase in contraction of alpha-toxin permeabilized preparations of tonic type smooth muscle.
abstract: The role of ATP newly synthesized from ADP and phosphocreatine (PC) by creatine kinase (CK) in the contraction of tonic type smooth muscle, rat femoral artery was studied, since its necessity for phasic type smooth muscle was previously shown. In alpha-toxin-permeabilized preparations obtained from rat femoral artery, Ca(2+) induced a tonic type contraction in the presence of ATP and PC. Omission of PC inhibited significantly the contraction. Treatment of the preparations with 2,4-dinitrofluorobenzene, an inhibitor of CK, also inhibited the contraction. In the presence of ADP and PC, Ca(2+) also induced the contraction to a level comparable to that in the presence of ATP and PC. The extent of phosphorylated myosin light chain was fairly consistent with that of Ca(2+)-induced contraction under all experimental conditions planned above. These results suggest that ATP newly synthesized by CK essentially participates in the whole of the contraction in tonic type smooth muscle, although it participates only in a rapid phasic contraction in phasic type muscle as previously shown.
authors.affiliation: Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University, Japan. takeuchi@vet.osakafu-u.ac.jp
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Muneyoshi
authors.initials: M
authors.lastname: Kitayama
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kushida
authors.forename: Akikazu
authors.initials: A
authors.lastname: Fujita
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hata
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis / Q000502:physiology*; D000818:Animals; D003402:Creatine Kinase / Q000096:biosynthesis*; D005263:Femoral Artery / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D010738:Type C Phospholipases / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
chemical_list: D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D010738:Type C Phospholipases