Further variability within the genus Crinivirus, as revealed by determination of the complete RNA genome sequence of Cucurbit yellow stunting disorder virus.
The complete nucleotide (nt) sequences of genomic RNAs 1 and 2 of Cucurbit yellow stunting disorder virus (CYSDV) were determined for the Spanish isolate CYSDV-AlLM. RNA1 is 9123 nt long and contains at least five open reading frames (ORFs). Computer-assisted analyses identified papain-like protease, methyltransferase, RNA helicase and RNA-dependent RNA polymerase domains in the first two ORFs of RNA1. This is the first study on the sequences of RNA1 from CYSDV. RNA2 is 7976 nt long and contains the hallmark gene array of the family Closteroviridae, characterized by ORFs encoding a heat shock protein 70 homologue, a 59 kDa protein, the major coat protein and a divergent copy of the coat protein. This genome organization resembles that of Sweet potato chlorotic stunt virus (SPCSV), Cucumber yellows virus (CuYV) and Lettuce infectious yellows virus (LIYV), the other three criniviruses sequenced completely to date. However, several differences were observed. The most striking novel features of CYSDV compared to SPCSV, CuYV and LIYV are a unique gene arrangement in the 3'-terminal region of RNA1, the identification in this region of an ORF potentially encoding a protein which has no homologues in any databases, and the prediction of an unusually long 5' non-coding region in RNA2. Additionally, the CYSDV genome resembles that of SPCSV in having very similar 3' regions in RNAs 1 and 2, although for CYSDV similarity in primary structures did not result in predictions of equivalent secondary structures. Overall, these data reinforce the view that the genus Crinivirus contains considerable genetic variation. Additionally, several subgenomic RNAs (sgRNAs) were detected in CYSDV-infected plants, suggesting that generation of sgRNAs is a strategy used by CYSDV for the expression of internal ORFs.
Aguilar
Juan M
JM
Estación Experimental 'La Mayora', Consejo Superior de Investigaciones Científicas, 29750 Algarrobo-Costa, Málaga, Spain.
Franco
Maribel
M
Estación Experimental 'La Mayora', Consejo Superior de Investigaciones Científicas, 29750 Algarrobo-Costa, Málaga, Spain.
Marco
Cristina F
CF
Estación Experimental 'La Mayora', Consejo Superior de Investigaciones Científicas, 29750 Algarrobo-Costa, Málaga, Spain.
Berdiales
Benjamín
B
Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
Rodriguez-Cerezo
Emilio
E
Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, 28049 Cantoblanco, Madrid, Spain.
Truniger
Verónica
V
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas, Campus Universitario de Espinardo, Apdo Correos 164, 30100 Espinardo, Murcia, Spain.
Aranda
Miguel A
MA
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas, Campus Universitario de Espinardo, Apdo Correos 164, 30100 Espinardo, Murcia, Spain.
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins; D029383:Crinivirus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D028464:Cucurbita / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D018840:HSP70 Heat-Shock Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020365:RNA Helicases; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase; D016415:Sequence Alignment; D013030:Spain
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D036022:Capsid Proteins; D018840:HSP70 Heat-Shock Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase; D020365:RNA Helicases