title: A novel erythromycin, 6-desmethyl erythromycin D, made by substituting an acyltransferase domain of the erythromycin polyketide synthase.
abstract: The acyltransferase (AT) domain in module 4 of the erythromycin polyketide synthase (PKS) was substituted with an AT domain from the rapamycin PKS module 2 in order to alter the substrate specificity from methylmalonyl-CoA to malonyl-CoA. The resulting strain produced 6-desmethyl erythromycin D as the predominant product. This AT domain swap completes the library of malonyl-CoA AT swaps on the erythromycin PKS and reinforces PKS engineering as a robust and generic tool.
authors.affiliation: Biotica Technology Limited, 181 A Huntingdon Road, Cambridge CB3 0DJ, UK.
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Petkovic
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Lill
authors.forename: Rose M
authors.initials: RM
authors.lastname: Sheridan
authors.forename: Barrie
authors.initials: B
authors.lastname: Wilkinson
authors.forename: Ellen L
authors.initials: EL
authors.lastname: McCormick
authors.forename: Hamish A I
authors.initials: HA
authors.lastname: McArthur
authors.forename: James
authors.initials: J
authors.lastname: Staunton
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
authors.forename: Steven G
authors.initials: SG
authors.lastname: Kendrew
mesh_terms: D000217:Acyltransferases; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D004917:Erythromycin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D009097:Multienzyme Complexes; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes; D004917:Erythromycin; D000217:Acyltransferases