Periodontal repair in dogs: a bioabsorbable calcium carbonate coral implant enhances space provision for alveolar bone regeneration in conjunction with guided tissue regeneration.
BACKGROUND
Collapse or compression of a barrier device into a periodontal defect or onto the root surface compromises outcomes following guided tissue regeneration (GTR). Bone biomaterials have been suggested to support regeneration of alveolar bone and to improve space provision with GTR devices. The objective of this study was to evaluate space provision, alveolar bone, and cementum regeneration following use of a bioabsorbable, calcium carbonate biomaterial in conjunction with GTR.


METHODS
Routine, critical size, 5 to 6 mm, supraalveolar, periodontal defects were created in 5 young adult beagle dogs. Alternate jaw quadrants in consecutive animals received GTR and the coral biomaterial (cGTR) or GTR alone. The animals were euthanized 4 weeks postsurgery and tissue blocks processed for histometric analysis.


RESULTS
The coral implant particles were surrounded by newly-formed bone or immersed in connective tissue and appeared to resorb and be replaced by bone. There was limited, if any, appreciable cementum regeneration. Space provision was enhanced in cGTR compared to GTR sites (6.1 +/- 1.6 versus 2.4 +/- 0.8 mm2; P<0.05). Bone regeneration (height) was significantly increased in cGTR compared to GTR sites averaging 1.9 +/- 0.6 and 1.2 +/- 0.6 mm, respectively (P<0.05). Bone regeneration (area) was 2-fold greater in cGTR sites compared to the GTR control (3.3 +/- 1.8 versus 1.4 +/- 0.5 mm2), however the difference was not statistically significant (P>0.05).


CONCLUSIONS
The coral implant significantly enhanced space provision for GTR while alveolar bone formation appeared to be enhanced by its use. Increased healing intervals are needed to fully understand the biologic value of the coral implant as an adjunct to GTR.
Wikesjö
Ulf M E
UM
Laboratory for Applied Periodontal and Craniofacial Regeneration, Department of Periodontology, Temple University, School of Dentistry, Philadelphia, PA 19140, USA. ulf.wikesjo@temple.edu
Lim
Won Hee
WH
Razi
Saghi S
SS
Sigurdsson
Thorarinn J
TJ
Lee
Michael B
MB
Tatakis
Dimitris N
DN
Hardwick
W Ross
WR
D020341:Absorbable Implants; D000539:Alveolar Process / Q000503:physiopathology; D000540:Alveolar Ridge Augmentation / Q000379:methods; D000818:Animals; D037421:Anthozoa; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D002119:Calcium Carbonate / Q000627:therapeutic use*; D003238:Connective Tissue / Q000503:physiopathology; D003739:Dental Cementum / Q000503:physiopathology; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D016555:Matched-Pair Analysis; D010012:Osteogenesis / Q000502:physiology; D012391:Root Resorption / Q000503:physiopathology; D020254:Tooth Ankylosis / Q000503:physiopathology; D014945:Wound Healing / Q000502:physiology
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002119:Calcium Carbonate