Multiple antibiotic resistance gene transfer from animal to human enterococci in the digestive tract of gnotobiotic mice.
It has been proposed that food animals represent the source of glycopeptide resistance genes present in enterococci from humans. We demonstrated the transfer of vanA and of other resistance genes from porcine to human Enterococcus faecium at high frequency in the digestive tract of gnotobiotic mice. Tylosin in the drinking water favored colonization by transconjugants.
Moubareck
C
C
Laboratoire de Microbiologie, UFR de Sciences Pharmaceutiques et Biologiques, Université René Descartes, 75270 Paris Cedex 06, France.
Bourgeois
N
N
Courvalin
P
P
Doucet-Populaire
F
F
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004064:Digestive System / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D013552:Swine; D015645:Tylosin / Q000494:pharmacology; D014881:Water Supply / Q000032:analysis
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015645:Tylosin