[Molecular mechanism of the sensitivity of myocardial cell injury after severe hypoxia].
OBJECTIVE
To explore the molecular mechanism of the sensitivity of myocardial cell injury after severe hypoxia.


METHODS
Differential expression of the genes in rat myocardium and skeletal muscles after severe hypoxia for 6 hours was determined by DNA chip containing 4096 mice genes.


RESULTS
There were high expressions of 125 genes in skeletal muscles and 111 genes in myocardial cells after 6 hours of severe hypoxia. Among them, high expression genes in myocardial cells included those encoding apoptosis activator Mtd, complement C3, vascular cell adhesion molecule-1, integrin beta subunit and lipopolysaccharide binding proteins (LBP).


CONCLUSIONS
Activation of apoptosis of myocardial cells and inflammatory mediators played important roles in myocardial cell injury after 6-hour severe hypoxia.
Wang
Shui-ming
SM
Institute of Pathology, Southwest Hospital, The Third Military Medical University, Chongqing, PR China.
Liu
You-sheng
YS
Chen
Rui
R
D000818:Animals; D002478:Cells, Cultured; D003176:Complement C3 / Q000378:metabolism*; D020869:Gene Expression Profiling; D000860:Hypoxia / Q000378:metabolism*; D039641:Integrin beta Chains / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
C111858:Bok protein, mouse; D003176:Complement C3; D039641:Integrin beta Chains; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1