[Analysis of subtype of porcine endogenous retrovirus in two species of Chinese pigs].
OBJECTIVE
To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs-Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six.


METHODS
Three primers which are specific for the three PERV subtypes (subtypes A, B and C) were used to amplify the genome DNA extracted from the pigs.


RESULTS
In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found.


CONCLUSIONS
In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate.
Zhang
Li
L
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Bu
Hong
H
Li
Shengfu
S
Yu
Ping
P
Li
Youping
Y
Cheng
Jingqiu
J
D000818:Animals; D017849:Animals, Inbred Strains; D004279:DNA, Viral / Q000235:genetics; D020077:Endogenous Retroviruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity; D013552:Swine / Q000821:virology*; D013556:Swine, Miniature; D015047:Zoonoses
D004279:DNA, Viral