Effects of virgin olive oil phenolic compounds on LDL oxidation and vasorelaxation activity.
This study examined the efficacy of virgin olive oil phenolic extract and other phenolic compounds (oleuropein, caffeic acid) in preventing oxidative modifications of human low density lipoprotein oxidised by CuCl2. The vasorelaxant effect of these compounds on rat aortic ring with and without functional endothelium is also discussed. Olive oil phenolic extract, caffeic acid and oleuropein increased the lag time of conjugated diene formation in a concentration-dependent manner. Moreover, phenolic extract produced a vasorelaxant effect that persisted in denuded aorta and after inhibition of nitric oxide synthase by NG-methyl-L-arginine (L-NMMA) or methylene blue. Oleuropein did not produce a relaxant effect, whereas caffeic acid produced partial relaxation at concentration 0.5 g/L.
Benkhalti
Fatiha
F
Département de Biologie, Unité de Recherche Nutrition-Santé, Faculté des Sciences Semlalia, Marrakech, Morocco.
Legssyer
Abdelkhaleq
A
Gómez
Puri
P
Paz
Elier
E
Lopez-Miranda
José
J
Perez-Jimenez
Francisco
F
el Boustani
Es Saddik
ES
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D008078:Cholesterol, LDL / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D000069463:Olive Oil; D010084:Oxidation-Reduction; D010636:Phenols / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D019323:omega-N-Methylarginine / Q000494:pharmacology
D008078:Cholesterol, LDL; D004791:Enzyme Inhibitors; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D019323:omega-N-Methylarginine; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat