title: FNR-mediated oxygen-responsive regulation of the nrdDG operon of Escherichia coli.
abstract: Transcription of the nrdDG operon, which encodes the class III nucleotide reductase, which is only active under anaerobic conditions, was strongly induced after a shift to anaerobiosis. The induction was completely dependent on the transcriptional activator FNR and was independent of the ArcA-ArcB two-component response regulator system. The nrdD transcript start site was mapped to a position immediately downstream of two FNR binding sites. Transcription of the other two nucleotide reductase operons, nrdAB and nrdEF, did not respond to oxygen conditions in a wild-type background, but nrdAB expression was increased in the fnr mutant under anaerobic conditions.
authors.affiliation: Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Boston
authors.forename: T
authors.initials: T
authors.lastname: Atlung
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D009876:Operon; D010100:Oxygen / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D014764:Viral Proteins; C490362:NrdG protein, E coli; D012264:Ribonucleotide Reductases; C101141:anaerobic ribonucleotide reductase; D010100:Oxygen