Gentamicin delivery to Burkholderia cepacia group IIIa strains via membrane vesicles from Pseudomonas aeruginosa PAO1.
When Pseudomonas aeruginosa PAO1 is treated with gentamicin, it releases membrane vesicles containing gentamicin (g-MVs) and peptidoglycan hydrolase, which makes the MVs bactericidal. We evaluate the ability of g-MVs to deliver gentamicin past the intrinsic permeability barrier of group IIIa Burkholderia cepacia and show that strain CEP0248 with low resistance to gentamicin is killed but the highly resistant strain C5424 is not. Immunoelectron microscopy revealed that gentamicin was delivered into both strains, suggesting that there might be another mechanism of resistance in C5424.
Allan
Nick D
ND
Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Beveridge
Terry J
TJ
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D018441:Biofilms; D016956:Burkholderia cepacia / Q000187:drug effects* / Q000648:ultrastructure; D016503:Drug Delivery Systems; D024881:Drug Resistance, Bacterial; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology*; D008566:Membranes / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D005839:Gentamicins