Experimental model of acute pancreatitis in Wistar rat: glucocorticoid treatment profile.
Severe acute pancreatitis may be triggered by an extrapancreatic insult at the peri-Vaterian duodenum such as that occurring in the short-term, 20 min closed duodenal loop model in Wistar rat, which mimics biliary acute pancreatitis or that following endoscopy. Glucocorticoids are immunological modulators whose therapeutic value is worth investigating. Wistar male rats were used under standardized conditions. Acute pancreatitis was induced by instillation of a 7% sodium tauraocholate solution with 5 drops of methylene blue to monitor absence of duodenal bilio pancreatic reflux into the peri-Vaterian duodenum for 20 min. Detection of biliopancreatic reflux with methylene blue was an exclusion criterion. Different doses and times of administration of subcutaneous hydrocortisone were evaluated. Biochemical assays were carried out in blood samples and pancreatic and lung tissue, while histpathological studies were done in the pancreas, lung liver, duodenum, spleen, kidneys, suprarenal glands, and stomach. Animals subjected to the experimental model developed severe acute pancreatitis. According to the dose and time of administration, hydrocortisone therapy was effective and beneficial at a dose of 4 mg/kg give 30 min before inducing acute pancreatitis. It was ineffective when doses were <4 mg/kg and given before sodium taurocholate harmful when the dose was >4 mg/kg and given either before or after. Thus, the proposed model is valid and useful to study the initiation mechanism of acute pancreatitis caused extrapancreatically while its amelioration by glucocorticoid is related the dose and time factor to achieve therapeutical results.
Cosen-Binker
Laura Iris
LI
Programa de Estudios Pancreáticos, Hospital de Clínicas, Universidad de Buenos Aires, Argentina.
Binker
Marcelo Gustavo
MG
Negri
Gustavo
G
Tiscornia
Osvaldo
O
D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002769:Cholelithiasis / Q000150:complications / Q000276:immunology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006854:Hydrocortisone / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D010195:Pancreatitis / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D016717:Sphincterotomy, Endoscopic / Q000009:adverse effects; D013656:Taurocholic Acid; D016896:Treatment Outcome
D000893:Anti-Inflammatory Agents; D013656:Taurocholic Acid; D006854:Hydrocortisone