title: Functional activity and ultrastructure of mitochondria isolated from myocardial apoptotic tissue.
abstract: Apoptosis in myocardial tissue slices was induced by extended incubation under anoxic conditions. Mitochondria were isolated from the studied tissue. A new method of isolation of mitochondria in special conditions by differential centrifugation at 1700, 10,000, and 17,000 g resulted in three fractions of mitochondria. According to the data of electron microscopy the heavy mitochondrial fraction (1700 g) consisted of mitochondrial clusters only, the middle mitochondrial fraction (10,000 g) consisted of mitochondria with typical for isolated mitochondria ultrastructure, and the light fraction consisted of small mitochondria (2 or 3 cristae) of various preservation. The heavy fraction contained unusual structural elements that we detected earlier in apoptotic myocardial tissue--small electron-dense mitochondria incorporated in bigger mitochondria. The structure of small mitochondria from the light fraction corresponded to that of the small mitochondria from these unusual elements--"mitochondrion in mitochondrion". The most important functions of isolated mitochondria are strongly inhibited when apoptosis is induced in our model. The detailed study of the activities of the two fractions of the apoptotic mitochondria showed that the system of malate oxidation is completely altered, the activity of cytochrome c as electron carrier is partly inhibited, while succinate oxidase activity is completely preserved (complexes II, III, and IV of the respiration chain). Succinate oxidase activity was accompanied by high permeability of the internal membrane for protons: the addition of uncoupler did not stimulate respiration. ATP synthesis in mitochondria was inhibited. We demonstrated that in our model of apoptosis cytochrome c remains in the intermembrane space, and, consequently, is not involved in the cascade of activation of effector caspases. The possible mechanisms of induction of apoptosis during anoxia are discussed.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119899, Russia. yag@genebee.msu.su
authors.forename: A A
authors.initials: AA
authors.lastname: Tonshin
authors.forename: V B
authors.initials: VB
authors.lastname: Saprunova
authors.forename: I M
authors.initials: IM
authors.lastname: Solodovnikova
authors.forename: L E
authors.initials: LE
authors.lastname: Bakeeva
authors.forename: L S
authors.initials: LS
authors.lastname: Yaguzhinsky
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D045304:Cytochromes c / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology* / Q000648:ultrastructure; D008293:Malates / Q000378:metabolism; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000648:ultrastructure; D008933:Mitochondrial Swelling / Q000502:physiology; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats
chemical_list: D008293:Malates; C030298:malic acid; D045304:Cytochromes c; D010088:Oxidoreductases; C022691:succinate oxidase; D020169:Caspases