[Effect of somatostatin on modulation of IL-10 and TGF-beta 1 during acute pancreatitis].
OBJECTIVE
To observe the effect of somatostatin on modulation of IL-10 and TGF-beta 1 in acute pancreatitis and investigate the mechanism.


METHODS
SD male rats were divided into 3 groups: Group 1, the normal rats as control (n = 6); Group 2, the rats with acute pancreatitis induced by ductual injection with 5% sodium cholate sulfur at the volume of 0.6 ml/kg without any other treatment (n = 8); Group 3, after pancreatitis were induced, the rats were injected hypodermically with somatostatin 3 micrograms/(kg.hr)(n = 6). The animals were killed at 2, 6 and 24 hours after operation (each period n = 7). The blood samples were taken for measurement of IL-10 and TGF-beta 1 (by ELISA). The weight of pancreatic tissue and amylopsin was also observed.


RESULTS
Both IL-10 and TGF-beta 1 in blood of acute pancreatitis increased significantly (P < 0.05). After the injection of somatostatin, IL-10 and TGF-beta 1 were found remarkably decreased at 24 hours postoperation (P < 0.05). No changes of the weight of pancreatic tissue and amylopsin were observed after the injection of somatostatin.


CONCLUSIONS
Somatostatin can depress the increase of inflammatory-associated cytokines (IL-10 and TGF-beta 1) that increased remarkably in acute pancreatitis, which is one of the most important reasons why somatostatin cannot relieve pancreatitis.
Wu
Hao
H
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Xiaoli
X
Deng
Yiyun
Y
Huang
Xinglan
X
Zhang
Zheng
Z
D000208:Acute Disease; D000818:Animals; D016753:Interleukin-10 / Q000097:blood*; D010195:Pancreatitis / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000097:blood*
D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D013004:Somatostatin