title: Crystallization and preliminary X-ray analysis of inorganic polyphosphate/ATP-glucomannokinase from Arthrobacter sp. strain KM.
abstract: Inorganic polyphosphate [poly(P)]/ATP-glucomannokinase from Arthrobacter sp. strain KM phosphorylates glucose and mannose, utilizing both ATP and poly(P) as phosphoryl donors. The enzyme was overexpressed in Escherichia coli, purified and crystallized by means of the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals were orthorhombic and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.2, b = 83.7, c = 103.8 A. Assuming two molecules per asymmetric unit, V(sol) is 0.49. X-ray diffraction data to 2.83 A have been collected from a single crystal.
authors.affiliation: Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Mukai
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawai
authors.forename: Shigetarou
authors.initials: S
authors.lastname: Mori
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization / Q000379:methods*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C485979:polyphosphate ATP-glucomannokinase