Distinct modes of floor plate induction in the chick embryo.
To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses of cell behaviour, we provide evidence that the principal contributor to the early neural midline, the future anterior floor plate, exists as a separate population of floor plate precursor cells in the epiblast of the gastrula stage embryo, and does not share a lineage with axial mesoderm. Analysis of the tissue interactions associated with differentiation of these cells to a floor plate fate reveals a role for the nascent prechordal mesoderm, indicating that more than one inductive event is associated with floor plate formation along the length of the neuraxis. We show that Nr1, a chick nodal homologue, is expressed in the nascent prechordal mesoderm and we provide evidence that Nodal signalling can cooperate with Shh to induce the epiblast precursors to a floor-plate fate. These results indicate that a shared lineage with axial mesoderm cells is not a pre-requisite for floor plate differentiation and suggest parallels between the development of the floor plate in amniote and anamniote embryos.
Patten
Iain
I
Centre for Developmental Genetics, Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK.
Kulesa
Paul
P
Shen
Michael M
MM
Fraser
Scott
S
Placzek
Marysia
M
D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D002642:Chick Embryo; D004627:Embryonic Induction / Q000502:physiology*; D004707:Endoderm / Q000502:physiology; D053823:Hedgehog Proteins; D008648:Mesoderm / Q000502:physiology; D055457:Nodal Protein; D020897:Organizers, Embryonic / Q000502:physiology; D015534:Trans-Activators / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
D053823:Hedgehog Proteins; D055457:Nodal Protein; D015534:Trans-Activators; D016212:Transforming Growth Factor beta