Anastellin, an FN3 fragment with fibronectin polymerization activity, resembles amyloid fibril precursors.
Anastellin is a carboxy-terminal fragment of the first FN3 domain from human fibronectin. It is capable of polymerizing fibronectin in vitro, and it displays anti-tumor, anti-metastatic and anti-angiogenic properties in vivo. We have determined the structure of anastellin using nuclear magnetic resonance spectroscopy and identified residues critical for its activity. Anastellin exhibits dynamic fluctuations and conformational exchange in solution. Its overall topology is very similar to the corresponding region of full-length FN3 domains. However, its hydrophobic core becomes solvent-accessible and some of its beta-strands lose their protection against hydrogen bonding to beta-strands from other molecules. These features seem to be relevant for the fibronectin polymerization activity of anastellin and resemble the characteristics of amyloid fibril precursors. We suggest that this analogy is not random and may reflect similarities between fibronectin and amyloid fibril formation.
Briknarová
Klára
K
The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037-1062, USA.
Akerman
Maria E
ME
Hoyt
David W
DW
Ruoslahti
Erkki
E
Ely
Kathryn R
KR
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry*; D002793:Cholic Acids / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013499:Surface Properties
D016564:Amyloid beta-Protein Precursor; D002793:Cholic Acids; D003902:Detergents; D005353:Fibronectins; D010446:Peptide Fragments; C423921:anastellin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate