KLP-18, a Klp2 kinesin, is required for assembly of acentrosomal meiotic spindles in Caenorhabditis elegans.
The proper segregation of chromosomes during meiosis or mitosis requires the assembly of well organized spindles. In many organisms, meiotic spindles lack centrosomes. The formation of such acentrosomal spindles seems to involve first assembly or capture of microtubules (MTs) in a random pattern around the meiotic chromosomes and then parallel bundling and bipolar organization by the action of MT motors and other proteins. Here, we describe the structure, distribution, and function of KLP-18, a Caenorhabditis elegans Klp2 kinesin. Previous reports of Klp2 kinesins agree that it concentrates in spindles, but do not provide a clear view of its function. During prometaphase, metaphase, and anaphase, KLP-18 concentrates toward the poles in both meiotic and mitotic spindles. Depletion of KLP-18 by RNA-mediated interference prevents parallel bundling/bipolar organization of the MTs that accumulate around female meiotic chromosomes. Hence, meiotic chromosome segregation fails, leading to haploid or aneuploid embryos. Subsequent assembly and function of centrosomal mitotic spindles is normal except when aberrant maternal chromatin is present. This suggests that although KLP-18 is critical for organizing chromosome-derived MTs into a parallel bipolar spindle, the order inherent in centrosome-derived astral MT arrays greatly reduces or eliminates the need for KLP-18 organizing activity in mitotic spindles.
Segbert
Christoph
C
Institut für Genetik, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
Barkus
Rosemarie
R
Powers
Jim
J
Strome
Susan
S
Saxton
William M
WM
Bossinger
Olaf
O
D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism* / Q000502:physiology; D018385:Centrosome / Q000378:metabolism / Q000502:physiology; D002875:Chromosomes / Q000378:metabolism / Q000502:physiology; D005260:Female; D019476:Insect Proteins / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D016547:Kinesins / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008540:Meiosis / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism* / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D008941:Spindle Apparatus / Q000378:metabolism* / Q000502:physiology
D019476:Insect Proteins; C092846:KLP2 protein, Bombyx mori; D034741:RNA, Small Interfering; D016547:Kinesins