title: Reperfusion injury after detorsion of unilateral ovarian torsion in rabbits.
abstract: OBJECTIVE
  To determine if reperfusion injury takes place in ovarian tissue following the detorsion of the torsioned ovary.
  
  
  METHODS
  Fifty-four New Zealand mature nonpregnant female rabbits were divided into six groups. One group served to determine the basal values of thiobarbituric acid reacting substance (TBARS) and another group was sham. In the third and fourth groups, ovarian torsions and subsequent oophorectomy were performed in 1 and 3h, respectively. In the fifth and sixth groups, detorsions were carried out after unilateral ovarian torsion lasting 1 and 3h, respectively, then oophorectomies of the detorsioned ovaries were performed 2h after detorsion. The level of TBARS in ovarian tissues was determined in all subjects. Statistical analysis was performed using analysis of variance and Duncan's multiple range test. Differences were considered to be significant if P<0.05.
  
  
  RESULTS
  The levels of TBARS were not different in the basal and sham groups (P>0.05), while ovarian torsion caused significant increase in TBARS in the ovary (P<0.05), and detorsion caused a further significant increase in ovarian TBARS (P<0.05).
  
  
  CONCLUSIONS
  Reperfusion injury in ovarian tissue, following the detorsion after the torsion of the ovary lasting up to 3h was demonstrated biochemically in this study.
authors.affiliation: Department of Obstetrics and Gynecology, Medical Faculty, Pamukkale University, Denizli, Turkey. bkaleli@netscape.net
authors.forename: Babur
authors.initials: B
authors.lastname: Kaleli
authors.forename: Erdal
authors.initials: E
authors.lastname: Aktan
authors.forename: Semra
authors.initials: S
authors.lastname: Gezer
authors.forename: Guldal
authors.initials: G
authors.lastname: Kirkali
mesh_terms: D000818:Animals; D005260:Female; D010049:Ovarian Diseases / Q000601:surgery*; D010052:Ovariectomy; D011817:Rabbits; D015427:Reperfusion Injury / Q000453:epidemiology / Q000209:etiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors; D014102:Torsion Abnormality / Q000601:surgery
chemical_list: D017392:Thiobarbituric Acid Reactive Substances