Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC.
The protective effect of Acanthopanax senticosus (AS) against ethanol (EtOH)-induced apoptosis of the human neuroblastoma cell line SK-N-MC was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 assay. It was shown that cells treated with EtOH exhibit classical apoptotic features, while cells pre-treated with Acanthopanax senticosus prior to EtOH exposure showed decreased occurrence of apoptotic features. In addition, Acanthopanax senticosus pre-treatment was shown to inhibit EtOH-induced increase in caspase-3 mRNA expression and activity. These results suggest that Acanthopanax senticosus may exert a protective effect against EtOH-induced apoptosis of human neuroblastoma cells.
Jang
Mi-Hyeon
MH
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
Shin
Min-Chul
MC
Kim
Youn-Jung
YJ
Kim
Chang-Ju
CJ
Chung
Joo-Ho
JH
Seo
Jung-Chul
JC
Kim
Ee-Hwa
EH
Kim
Kyu-Yeol
KY
Lee
Choong-Yeol
CY
Kim
Kyeong-Mi
KM
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D028001:Eleutherococcus; D000431:Ethanol; D005434:Flow Cytometry; D006801:Humans; D009447:Neuroblastoma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D000431:Ethanol; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; C022616:thiazolyl blue