[Transformation of S- (+)-ofloxacin enantiomer in human body and determination by synchronous-derivative fluorimetry].
At pH 3,S,R enantioners of ofloxacin can be well recognized by the synchronous-first derivative fluorimetry. Based on this, a new method for the recognition and determination of enantiomers of ofloxacin, the synchronous-first order derivative fluorimetry has been developed. The linear range is 0.36-2.16 micrograms.mL-1(R), 0.36-2.89 micrograms.mL-1(S), and 3.16-31.6 micrograms.mL-1(S). The detection limit is 0.36 microgram.mL-1, RSD is less than 6.6%, and the recovery is within the range of 97%%-104%. The transformation of S- to R- (+)-ofloxacin enantiomer in human body is founded. In 0-3 h after taking ofloxacin tablets by a healthy subject, R-enantiomer can not be detected. At the 6th hour, remarkable amount of R-enantiomer was detected, and then R-/S-enantiomer ratio increased rapidly to 21.3% at the 24th hour for 2.1% at the 6th hour.
Gong
Q J
QJ
Department of Chemistry, University of Shanxi, Taiyuan 030006, China.
Jin
W J
WJ
Dong
C
C
D000890:Anti-Infective Agents / Q000493:pharmacokinetics* / Q000652:urine; D005470:Fluorometry / Q000379:methods; D006801:Humans; D015242:Ofloxacin / Q000493:pharmacokinetics* / Q000652:urine; D013050:Spectrometry, Fluorescence / Q000379:methods; D013237:Stereoisomerism
D000890:Anti-Infective Agents; D015242:Ofloxacin