title: Antioxidant compounds from the leaves of Peucedanum japonicum thunb.
abstract: Seventeen compounds were isolated from the n-butanol soluble fraction of the leaves of Peucedanum japonicum Thunb. On the basis of MS and various NMR spectroscopic techniques, the structures of the isolated compounds were determined as isoquercitrin (1), rutin (2), 3-O-caffeoylquinic acid (3), 4-O-caffeoylquinic acid (4), 5-O-caffeoylquinic acid (5), cnidioside A (6), praeroside II (7), praeroside III (8), apterin (9), esculin (10), (R)-peucedanol (11), (R)-peucedanol 7-O-beta-d-glucopyranoside (12), l-tryptophan (13), uracil (14), guanosine (15), uridine (16), and thymidine (17). All compounds except 11 and 12 were isolated for the first time from P. japonicum. Several isolated compounds were quantified by high-performance liquid chromatography analysis. In addition, all isolated compounds were examined for radical scavenging on 1,1-diphenyl-2-picrylhydrazyl radical and for inhibition of oxidation of liposome induced by 2,2'-azobis(2-amidinopropane)dihydrochloride. Compounds 2-5 were found to be the major potent constituents, which contribute to the antioxidant activity of P. japonicum leaves.
authors.affiliation: Division of Food and Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-8585, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Hisamoto
authors.forename: Hiroe
authors.initials: H
authors.lastname: Kikuzaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohigashi
authors.forename: Nobuji
authors.initials: N
authors.lastname: Nakatani
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers