Protein promoting vibrations in enzyme catalysis--a conserved evolutionary motif.
A computational method to identify residues important in creating a protein promoting vibration (PPV) in enzymes was previously developed and applied to horse liver alcohol dehydrogenase (HLADH), resulting in the identification of eight important residues. From these residues, we define a sequence motif, the PPV generating sequence, and find it to be unique and general to a larger group of alcohol dehydrogenases from diverse sources, demonstrating that nature has selected for the PPV generating sequence.
Mincer
Joshua S
JS
Schwartz
Steven D
SD
D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D030562:Databases, Protein; D004798:Enzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D006736:Horses; D006801:Humans; D051379:Mice; D010944:Plants / Q000201:enzymology / Q000235:genetics; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D013816:Thermodynamics
D004798:Enzymes; D000426:Alcohol Dehydrogenase