title: Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of human growth hormone.
abstract: The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison. The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique. The composite microspheres were spherical in shape (44.6 +/- 2.47 microm), and the PLGA-mannitol microspheres were 39.7 +/- 2.50 microm. Drug-loading efficiency varied from 93.2% to 104%. The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres. FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres. The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.
authors.affiliation: Hacettepe University, Ankara, Turkey.
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Capan
authors.forename: Ge
authors.initials: G
authors.lastname: Jiang
authors.forename: Stefano
authors.initials: S
authors.lastname: Giovagnoli
authors.forename: Kyu-Heum
authors.initials: KH
authors.lastname: Na
authors.forename: Patrick P
authors.initials: PP
authors.lastname: DeLuca
mesh_terms: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016503:Drug Delivery Systems; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D019382:Human Growth Hormone; D011100:Polyglycolic Acid