Interleukin-10 expression is closely correlated with the expression of granulocyte-macrophage colony-stimulating factor in non-small cell lung cancer.
It is unclear how the cytokine network contributes to the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in non-small cell lung cancer (NSCLC). We examined the gene expression of GM-CSF and interleukin (IL)-10 in 78 surgical specimens and 16 xenografts of NSCLC by reverse transcription-polymerase chain reaction. Forty-four of the 78 (56.4%) surgical specimens and 11 of the 16 (68.8%) xenografts of NSCLC showed GM-CSF gene expression. Forty-one of the 44 (93.2%) GM-CSF-positive NSCLC specimens showed IL-10 expression, and GM-CSF expression was significantly correlated with IL-10 expression (p < 0.0171, Mann-Whitney's U-test). These results suggested that IL-10 is one of the cytokines up-regulating the GM-CSF expression in NSCLC.
Kamiya
Takashi
T
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Hatanaka
Hiroyuki
H
Abe
Yoshiyuki
Y
Kijima
Hiroshi
H
Yamazaki
Hitoshi
H
Ohnishi
Yasuyuki
Y
Inoue
Hiroshi
H
Ueyama
Yoshito
Y
Osamura
Yoshiyuki
Y
Nakamura
Masato
M
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D053701:Receptors, Interleukin-10; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D015854:Up-Regulation
D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor