Survival rate and antioxidant enzyme activity of ram spermatozoa after dilution with different extenders or selection by a dextran swim-up procedure.
The aim of this study was to evaluate the effect of four extenders (Sucrose (S), Galactose (G), milk-yolk (MY), and Fiser (F)) on the motility, membrane integrity, and functional integrity of ram spermatozoa during liquid storage at 15 degrees C. The use of either S or MY for the selection of high quality spermatozoa by a swim-up procedure was comparatively analyzed. Additionally, the activity of three antioxidant enzymes, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) was evaluated in both swim-up selected samples maintained at 15 degrees C for 6h. Sperm motility was better preserved in MY and was significantly higher after 6h of incubation than in either S or F (P<0.0001) and G (P<0.0005). Likewise, the incidence of spermatozoa with integral and functional membranes was higher in samples diluted in MY, with no significant decrease after 6h of incubation. The comparative analysis of the swim-up procedure performed with either MY or S revealed that not only was total sperm recovery significantly (P<0.001) higher (67.3%+/-3.21 versus 47.6%+/-3.78), but also that the best survival rate of spermatozoa was found in the MY stored sample. Sperm motility, viability and response to a hypoosmotic swelling (HOS) test were also significantly higher in the MY extended sample, maintaining still significantly higher values after 6h of incubation. In addition, this sample showed higher activity values for the antioxidant defense enzyme system.
Martí
J I
JI
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, 50013 Zaragoza, Spain.
Martí
E
E
Cebrián-Pérez
J A
JA
Muiño-Blanco
T
T
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002469:Cell Separation; D002470:Cell Survival; D003911:Dextrans; D005690:Galactose; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008297:Male; D008892:Milk; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D012996:Solutions; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology* / Q000502:physiology*; D013395:Sucrose; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D003911:Dextrans; D012996:Solutions; D013395:Sucrose; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005690:Galactose