title: Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes.
abstract: We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.
authors.affiliation: Department of Chemistry, University of Southampton, Highfield, Southampton, UK S017 1BJ.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: McKeen
authors.forename: Lynda J
authors.initials: LJ
authors.lastname: Brown
authors.forename: Jamie T G
authors.initials: JT
authors.lastname: Nicol
authors.forename: John M
authors.initials: JM
authors.lastname: Mellor
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D019793:Fluorescein / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008954:Models, Biological; D008956:Models, Chemical; D015341:Nucleic Acid Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D016133:Polymerase Chain Reaction
chemical_list: D001391:Azo Compounds; D017931:DNA Primers; D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D009841:Oligonucleotides; C008492:methyl red; D019793:Fluorescein