Expression of peroxisome proliferator-activated receptor and CCAAT/enhancer binding protein transcription factors in cultured human sebocytes.
Lipid synthesis and accumulation represent a major step in sebocyte differentiation and it may be of importance for sebocytes to express two families of transcription factors, CCAAT/enhancer binding proteins (c/EBPs) and peroxisome proliferator-activated receptors (PPARs), which were found to play a crucial role in the differentiation of adipocytes. Using the immortalized human sebaceous gland cell line SZ95 we examined the expression of the molecules before and after treatment with testosterone, 5alpha-dihydrotestosterone, dexamethasone, 17beta-estradiol and genistein, at 6, 12, 24, and 48 h, respectively. Reverse transcription-PCR analysis showed expression of peroxisome proliferator-activated receptors -alpha, -delta, -gamma1, -gamma2 and CCAAT/enhancer binding proteins-alpha, -beta, -gamma-delta in native SZ95 sebocytes. In western blot studies, high levels of CCAAT/enhancer binding proteins-alpha and -beta, and peroxisome proliferator-activated receptors-gamma were expressed at 6, 24, and 12 h, respectively. Immunostaining of the cultured sebocytes showed the CCAAT/enhancer binding proteins-alpha and -beta mainly localized within nuclei, whereas peroxisome proliferator-activated receptors-gamma in the cytoplasm. Strong staining of sebocytes was immunohistochemically revealed in the basal layer of sebaceous glands in human scalp and sebaceous nevus. Genistein down-regulated the expression of CCAAT/enhancer binding proteins-alpha and -beta, and peroxisome proliferator-activated receptors-gamma on the protein level. Treatment with linoleic acid for 48 h induced further differentiation of sebocytes leading to abundant lipid synthesis.
Chen
WenChieh
W
Department of Dermatology, College of Medicine, National Cheng Kung University, Sheng-Li Road 138, 704 Tainan, Taiwan. wchen@mail.ncku.edu.tw
Yang
Chao-Chun
CC
Sheu
Hamm-Ming
HM
Seltmann
Holger
H
Zouboulis
Christos C
CC
D000970:Antineoplastic Agents / Q000494:pharmacology; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D051743:CCAAT-Enhancer-Binding Protein-delta; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology; D002461:Cell Line, Transformed; D003907:Dexamethasone / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D019833:Genistein / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D012627:Sebaceous Glands / Q000166:cytology*; D013739:Testosterone / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*
D000970:Antineoplastic Agents; D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D022762:CCAAT-Enhancer-Binding Proteins; C405462:CCAAT-enhancer-binding protein-gamma; C497252:CEBPD protein, human; D004967:Estrogens; D005938:Glucocorticoids; D012739:Gonadal Steroid Hormones; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D013196:Dihydrotestosterone; D051743:CCAAT-Enhancer-Binding Protein-delta; D013739:Testosterone; D003907:Dexamethasone; D019833:Genistein