Chemical modification of milk xanthine oxidase with different modifiers.
Xanthine oxidase (XO), purified from buttermilk was subjected to modification with N-phenylmaleimide, p-toluene-sulfonyl chloride, and 2-mercaptobenzimidazole. Spectrophotometric monitoring of the enzyme before and after treatment with these modifiers are presented. The results show that the interaction of XO with the modifiers was accompained by a change in UV absorption, as compared with untreated enzyme. The data indicate that these modifiers caused conformational changes in the polypeptide chain of milk XO due to interaction of these modifiers with sulfahydryl and/or hydroxyl groups. Moreover, the modifiers induce uptake inhibition of milk XO and appeared to be dependant upon either the concentration or incubation time.
Aboul-Enein
Hassan Y
HY
Pharmaceutical Analysis Laboratory, Biological and Medical Department (MBC 03-65), King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
Refaie
Mohamed O I
MO
El-Gazzar
Hayam
H
El-Aziz
Magda Abd
MA
D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D008892:Milk / Q000201:enzymology*; D015394:Molecular Structure; D014105:Tosyl Compounds / Q000737:chemistry; D014969:Xanthine Oxidase / Q000737:chemistry*
D001562:Benzimidazoles; D008301:Maleimides; D014105:Tosyl Compounds; C025506:4-toluenesulfonyl chloride; C030698:2-mercaptobenzimidazole; C028525:N-phenylmaleimide; D014969:Xanthine Oxidase