In vitro selection for enzymatic activity: a model study using adenylate cyclase.
An in vitro enzyme selection that can, in principle, be generalised to most chemical reactions, is described. It makes use of filamentous phage display and of a tailor-made antibody fragment directed against the reaction product. The conversion of ATP into 3',5'-cyclic AMP catalysed by Bordetella pertussis adenylate cyclase is taken as an example.
Strobel
Heike
H
Unité de Chimie Organique, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris 15, France.
Ladant
Daniel
D
Jestin
Jean-Luc
JL
D000262:Adenylyl Cyclases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001886:Bordetella pertussis / Q000201:enzymology; D000242:Cyclic AMP / Q000737:chemistry / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000378:metabolism; D015394:Molecular Structure; D019151:Peptide Library
D001426:Bacterial Proteins; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D000242:Cyclic AMP; D000262:Adenylyl Cyclases