title: HPLC determination of imidazole antimycotis in antidandruff cosmetic products.
abstract: A simple HPLC method for the determination of imidazole antimycotics in cosmetic antidandruff formulations has been developed. HPLC was carried out on a Discovery RP-Amide C16 column and spectrophotometric detection was performed at 220 nm. The initial mobile phase was a mixture of acetonitrile and aqueous 10(-3) M NaClO4 (pH 3.0) in the ratio of 15:85 (v/v); then a linear gradient up to 46% acetonitrile in 70 min, and up to 50% in 80 min. The extraction procedure has been validated by analyzing samples of shampoo and lotion spiked with 1% of the active principles. The recoveries were greater than 95% and the reproducibility was within 3%.
authors.affiliation: Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanitá, Rome, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Gagliardi
authors.forename: Daniela
authors.initials: D
authors.lastname: De Orsi
authors.forename: Paola
authors.initials: P
authors.lastname: Chimenti
authors.forename: Rita
authors.initials: R
authors.lastname: Porra'
authors.forename: Domenica
authors.initials: D
authors.lastname: Tonelli
mesh_terms: D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000935:Antifungal Agents; D003358:Cosmetics; D007093:Imidazoles; D007202:Indicators and Reagents