title: [Screening for proteins interacting with novel gene AngRem104 in the adult human kidney cDNA library by yeast two hybrid system].
abstract: OBJECTIVE
  To screen for proteins interacting with novel gene AngRem104.
  
  
  METHODS
  The yeast strain AH109 was transformed with AngRem104-pGBKT7/c-myc, and yeast-mating was utilized to screen for interacting proteins with AngRem104 in pretransformed human kidney cDNA library.
  
  
  RESULTS
  AngRem104 protein was expressed and there was no self-activation and toxicity in AH109. Seven proteins that interacted with AngRem104, including eukaryotic translation elongation factor 1 alpha 1, glucocorticoid receptor AF-1 specific elongation factor, beta 2-microglobulin, BBS2 and four of cell metabolism related proteins, were identified.
  
  
  CONCLUSIONS
  The novel gene AngRem104 may interact with the proteins, which may interfere with cell metabolism, disturb the balance between transcription factors, or may be involved in some immunity related and hereditary diseases. This investigation provides functional clues for further exploration of the novel gene, AngRem104.
authors.affiliation: Department of Nephrology, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiubin
authors.initials: X
authors.lastname: Liang
authors.forename: Ping
authors.initials: P
authors.lastname: Hou
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D007668:Kidney; D011506:Proteins; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D011506:Proteins