title: Impact of osmotic stress on volume regulation, cytoplasmic solute composition and lysine production in Corynebacterium glutamicum MH20-22B.
abstract: The response of the L-lysine producing Corynebacterium glutamicum strain MH20-22B to osmotic stress was studied in batch cultures. To mimic the conditions during a fermentation process the long term adaptation of cells subjected to a constant osmotic stress between 1.0 and 2.5 osM was investigated. Cytoplasmic water content and volume of C. glutamicum cells were found to depend on growth phase, extent of osmotic stress and availability of betaine. The maximal cytoplasmic volumes, which were highest at maximal growth rate, were linearily related to osmotic stress, whereas in stationary cells no active volume regulation was observed. Under severe osmotic stress proline was the prominent compatible solute in growing cells. Uptake of betaine, if available in the medium, reduced the concentration of proline from 750 to 300 mM, indicating that uptake of compatible solutes is preferred to synthesis. Furthermore, betaine was shown to have a higher efficiency to counteract osmotic stress, since the overall concentration of compatible solutes was lower in the presence of betaine. Under severe osmotic stress, the addition of betaine shifted L-lysine production in MH20-22B to earlier fermentation times and increased both product concentration and yield in these phases, but did not improve the final L-lysine yield.
authors.affiliation: Institut für Biochemie der Universität zu Köln, D-50674 Köln, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Rönsch
authors.forename: Reinhard
authors.initials: R
authors.lastname: Krämer
authors.forename: Susanne
authors.initials: S
authors.lastname: Morbach
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D001622:Betaine / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D003352:Corynebacterium / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D008239:Lysine / Q000096:biosynthesis*; D012996:Solutions; D014867:Water / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D003470:Culture Media; D012996:Solutions; D014867:Water; D001622:Betaine; D008239:Lysine