[Relation of heparanase mRNA expression and angiogenesis to the progression of gastric carcinoma].
OBJECTIVE
To investigate the relation of heparanase gene and angiogenesis with the progress of gastric carcinoma (GC).


METHODS
Expression of heparanase mRNA in 52 GC tissue was detected by in situ hybridization assay. Microvessel density (MVD) was examined by immunohistochemical method. MVD and heparanase mRNA expression were analysed with their relation to histological grade, invasion depth,lymph node metastasis and organ metastasis.


RESULTS
MVD was 73.2 +/- 22.8 in 25 (48.1%) tissue with positive heparanase mRNA. It was 44.8 +/- 11.9 in 27 (51.9%) tissues with negative heparanase mRNA, between which the difference was significant (P < 0.001). MVD and heparanase mRNA expression were related with lymph node metastasis and depth of serosal invasion in GC (P < 0.005).


CONCLUSIONS
Heparanase, being related to angiogenesis in gastric cancer, promotes growth, invasion and metastasis. Heparanase mRNA expression is an important predictor of the biological behavior of human gastric carcinoma.
Ren
Xiao-long
XL
Department of Ultrasonography, Lanzhou First People Hospital, Lanzhou 730050, China.
Zhu
Ren-zhi
RZ
Wang
Li-jing
LJ
Zhang
Ming-li
ML
Li
Dong-hai
DH
Deng
Xiao-qun
XQ
D000230:Adenocarcinoma / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D000328:Adult; D000368:Aged; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology; D015972:Gene Expression Regulation, Neoplastic; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D000099175:Heparanase
D005966:Glucuronidase; D012333:RNA, Messenger; D000099175:Heparanase