[The influence of selected stimulators on chemiluminescence of neutrophils in whole blood in children with bronchial asthma].
In the research the assessment of oxygen burst of neutrophils was presented, tested by chemiluminescence test, in full blood, in children suffering from atopic bronchial asthma. The examination included 34 children (aged 4-17 years) suffering from atopic bronchial asthma with positive individual, family and also clinical anamnesis as to atopy. In all cases also an increased serum level of IgE was found. The patients were divided into two groups: I-11 children with asthma, II-23 children with asthma complicated with recurrent respiratory infection (RRI). The control group consisted of 15 healthy volunteers. We estimated the spontaneous and stimulated (Zymosan, fMLP, PMA) luminolo-dependent chemiluminescence using Microlumat LB 96P (RG&G Brethold, Germany) on microplates (LB 96P-WMP). An increased spontaneous CL was demonstrated in both groups as compared to the control one. Also statistically significant differences in the maximal values of CL were observed after Zymosan, fMLP, PMA stimulation in children with asthma, with asthma and rri, compared to the control group. The most potent stimulator of oxygen burst was Zymosan.
Lewandowicz-Uszyńska
Aleksandra
A
Katedra Propedeutyki Pediatrii Klinika Immunologii Wieku Rozwojowego Akademii Medycznej we Wrocławiu. alusz@dhnet.wroc.pl
D000293:Adolescent; D001249:Asthma / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D015054:Zymosan / Q000378:metabolism
D007073:Immunoglobulin E; D015054:Zymosan