Molecular basis of Fas and cytochrome c pathways of apoptosis induced by tartary buckwheat flavonoid in HL-60 cells.
In a previous study, we showed that tartary buckwheat flavonoid (TBF) induced HL-60 leukemic cell apoptosis, most likely via a caspase 3 activating pathway. The aim of this study was to further investigate the molecular mechanisms involved in TBF-induced apoptosis of HL-60 cells. Thus, death receptor Fas expression on HL-60 cells was detected by flow cytometry (FCM). We also studied the effect of TBF on intranuclear DNA binding activity of NF-kappaB, as well as release of mitochondrial cytochrome c into the cytosol in HL-60 cells by FCM. The results suggest that TBF-induced apoptosis of HL-60 cells may be stimulated by the release of cytochrome c to the cytosol, upregulation of Fas expression on the cell surface, and through a caspase-3-dependent mechanism. Furthermore, TBF-induced apoptosis may be partly regulated through the inactivation of NF-kappaB in HL-60 cells. The induction of apoptosis by TBF may be attributed to its cancer chemopreventive activity.
Ren
W
W
Department of Hematology, Shanxi Medical University, Shanxi, P.R. China.
Qiao
Z
Z
Wang
H
H
Zhu
L
L
Zhang
L
L
Lu
Y
Y
Zhang
Z
Z
Wang
Z
Z
D017209:Apoptosis; D003574:Cytochrome c Group / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011485:Protein Binding; D019014:fas Receptor / Q000096:biosynthesis*
D003574:Cytochrome c Group; D005419:Flavonoids; D016328:NF-kappa B; D019014:fas Receptor; D004247:DNA