title: Optical sectioning by two-pinhole confocal fluorescence microscopy.
abstract: A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.
authors.affiliation: Departamento de Optica, Universidad de Valencia, Burjassot 46100, Spain. manuel.martinez@uv.es
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Corral
authors.forename: M T
authors.initials: MT
authors.lastname: Caballero
authors.forename: C
authors.initials: C
authors.lastname: Ibáñez-López
authors.forename: V
authors.initials: V
authors.lastname: Sarafis
mesh_terms: D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D013382:Subtraction Technique / Q000295:instrumentation*