title: An ultra acid stable reversed stationary phase.
abstract: We report a new reversed phase liquid chromatography (RPLC) phase with remarkable acid stability using dimethyl(ethylphenylchloromethyl)chlorosilane (1), oligomeric polystyrene (PS), and octylbenzene (C8). This phase Si-1-PS-C8 was prepared using silica modification processes and Friedel-Crafts alkylation chemistry. Under highly aggressive mobile phase conditions, Si-1-PS-C8 exhibited remarkable stability as evinced by only minimal reduction in retention factor (k') after 1400 column volumes at pH = 0.5 and 150 degrees C. The peak shapes for a variety of basic solutes were symmetric using Si-1-PS-C8. Evidence for a highly cross-linked coating of the silica particles was observed using scanning electron microscopy. The remarkable stability of this phase is unparalleled as compared to all other RPLC phases reported to date.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455-0431, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Trammell
authors.forename: Lianjia
authors.initials: L
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.forename: Marc A
authors.initials: MA
authors.lastname: Hillmyer
authors.forename: Peter W
authors.initials: PW
authors.lastname: Carr
mesh_terms: D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012821:Silanes; D012822:Silicon Dioxide