title: The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated from tissues and faeces.
abstract: The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated in BACTEC cultures from clinical samples was assessed by spiking tissues and faeces at various points during the decontamination procedure. Routine protocols in the laboratory were shown to decrease the number of organisms isolated per sample by about 2.7 log(10) and 3.1 log(10) for faeces and tissues, respectively. These findings are important for the interpretation of negative culture results and may be useful in epidemiological studies. Addition of a centrifugation step to the tissue protocol increased the recovery by about 1 log, but resulted in increased contamination of BACTEC cultures. These studies may also facilitate future improvements to decontamination procedures.
authors.affiliation: Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, PMB 8, Camden, NSW, Australia. leslie.reddacliff@agric.nsw.gov.au
authors.forename: L A
authors.initials: LA
authors.lastname: Reddacliff
authors.forename: A
authors.initials: A
authors.lastname: Vadali
authors.forename: R J
authors.initials: RJ
authors.lastname: Whittington
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000662:veterinary; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology; D007082:Ileum / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*