title: Expression of midkine and receptor-like protein tyrosine phosphatase (RPTP)-beta genes in the rat stomach and the influence of rebamipide.
abstract: BACKGROUND
  Midkine has been reported to bind to receptor-like protein tyrosine phosphatase (RPTP)-beta and to play important roles in growth and differentiation of various cells. Midkine is expressed in rat stomach during experimental ulcer healing, suggesting that the midkine-RPTP-beta system has some physiological functions in the stomach. Rebamipide is a mucoprotective drug used for the treatment of gastric ulcers. We have tested the hypothesis that the ulcer healing mechanism stimulated by rebamipide is linked physiologically to the gastric midkine-RPTP-beta system.
  
  
  METHODS
  Seven-week-old-male Wistar rats were used. Midkine and RPTP-beta gene expression in rat stomach was investigated by laser capture microdissection coupled with the reverse transcription-polymerase chain reaction (RT-PCR). The effects of rebamipide on midkine and RPTP-beta expression in rat stomach and the gastric epithelial cell line RGM1 were evaluated by RT-PCR and Northern blot analyses.
  
  
  RESULTS
  Midkine and RPTP-beta expression was detected in the gastric mucosal, submucosal and muscle layers. Rebamipide stimulated both midkine and RPTP-beta expression in rat stomach and RGM1 cells.
  
  
  CONCLUSIONS
  Rebamipide may protect the gastric mucosa by regulating midkine and RPTP-beta expression.
authors.affiliation: Department of Internal Medicine II, Shimane Medical University, Shimane, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yuki
authors.forename: S
authors.initials: S
authors.lastname: Ishihara
authors.forename: M
authors.initials: M
authors.lastname: Rumi
authors.forename: F
authors.initials: F
authors.lastname: Ortega-Cava Cesar
authors.forename: Y
authors.initials: Y
authors.lastname: Kadowaki
authors.forename: H
authors.initials: H
authors.lastname: Kazumori
authors.forename: M
authors.initials: M
authors.lastname: Yuki
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: Y
authors.initials: Y
authors.lastname: Miyaoka
authors.forename: N
authors.initials: N
authors.lastname: Yoshino
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D000078792:Midkine; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D000897:Anti-Ulcer Agents; D002352:Carrier Proteins; D016207:Cytokines; D009419:Nerve Tissue Proteins; D015363:Quinolones; D000078792:Midkine; D012313:RNA; D017027:Protein Tyrosine Phosphatases; C516852:Ptprg protein, rat; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; C052785:rebamipide; D000409:Alanine