Effects of carbohydrate availability on sustained shivering II. Relating muscle recruitment to fuel selection.
The purpose of this study was to quantify how shivering activity would be affected by large changes in fuel metabolism (see Haman F, Peronnet F, Kenny GP, Doucet E, Massicotte D, Lavoie C, and Weber J-M, J Appl Physiol 96: 000-000, 2004). Adult men were exposed to 10 degrees C for 2 h after a low-carbohydrate diet and exercise (Lo) and after high-carbohydrate diet without exercise (Hi). Using simultaneous metabolic and electromyographic (EMG) measurements, we quantified the effects of changes in fuel selection on the shivering activity of eight large muscles representing >90% of total shivering muscle mass. Contrary to expectation, drastic changes in fuel metabolism [carbohydrates 28 vs. 65% of total heat production (Hprod), lipids 53 vs. 23% Hprod, and proteins 19 vs. 12% Hprod for Lo and Hi, respectively] are achieved without altering the EMG signature of shivering muscles. Results show that total shivering activity and the specific contribution of each muscle to total shivering activity are not affected by large changes in fuel selection. In addition, we found that changes in burst shivering rate ( approximately 4 bursts/min), relative contribution of burst activity to total shivering ( approximately 10% of total shivering activity), and burst shivering intensity ( approximately 12% of maximal voluntary contraction) are the same between Lo and Hi. Spectral analysis of EMG signals also reveals that mean frequencies of the power spectrum remained the same under all conditions (whole body average of 78 +/- 5 Hz for Lo and 83 +/- 7 Hz for Hi). During low-intensity shivering, humans are therefore able to sustain the same thermogenic rate by oxidizing widely different fuel mixtures within the same muscle fibers.
Haman
François
F
Biology Dept., Univ. of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada K1N 6N5. jmweber@science.uottawa.ca
Legault
Stéphane R
SR
Rakobowchuk
Mark
M
Ducharme
Michel B
MB
Weber
Jean-Michel
JM
D000328:Adult; D004040:Dietary Carbohydrates / Q000493:pharmacokinetics*; D004576:Electromyography; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D006003:Glycogen / Q000378:metabolism*; D006801:Humans; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D012768:Shivering / Q000187:drug effects / Q000502:physiology*; D012881:Skin Temperature
D004040:Dietary Carbohydrates; D006003:Glycogen