[Identification of fungi in tissues and cultures: the importance of argirophilic substances on its cellular walls].
An experimental development based on the combination of microwaves action with one of the methods of silver staining by Del Río Hortega is presented. Material from pathological tissues and culture of fungi were studied. Besides morphological studies, were considered the causes of reduction from ionic to metalic silver, some characteristics of the silver reagent and its relationship with histochemical constitution of cellular walls. It is pointed the rapidity in fungi demonstration, the satisfactory definition of affected tissues, the advantages of working with a stable reagent, the omission of carcinogenetic substances, the possibility of stain fungical structures in previously stained materials with anilinic techniques, and the extent of the method to cultured materials without necessity of previous formaldehidic fixation.
Piva
J R
JR
Servicio de Análisis por Imágenes, Facultad de Cs. Veterinarias, Univ. Nac. del Litoral. rampi@fadu.unl.edu.ar
Ortega
H H
HH
Canal
A M
AM
Piva
C E
CE
Reus
V
V
Seib
E P
EP
D000328:Adult; D000818:Animals; D001706:Biopsy; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005658:Fungi / Q000302:isolation & purification*; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D008297:Male; D008872:Microwaves; D008875:Middle Aged; D051381:Rats; D016622:Silver Staining / Q000379:methods*; D012867:Skin / Q000382:microbiology
D003470:Culture Media