title: Short tandem repeat typing in exon 1 of the androgen receptor gene.
abstract: Exon 1 of the androgen receptor (AR) gene on the X chromosome contains a polymorphic CAG trinucleotide short tandem repeat. We describe here the rapid and reliable method of typing CAG repeats using electrophoresis, with denaturing polyacrylamide gel and an allelic ladder marker. Twenty-one alleles (the repeat number ranges from 13 to 35) were found using CAG repeat typing in normal Japanese individuals (83 males and 82 females). The allelic diversity (h) calculated was h=0.889, illustrating that CAG repeats at the AR locus is a highly polymorphic system.
authors.affiliation: Department of Legal Medicine, Ehime University School of Medicine, Ehime 791-0295, Japan. nishimuk@m.ehime-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Fujii
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Okiura
authors.forename: Koji
authors.initials: K
authors.lastname: Nishimura
authors.forename: Chitoshi
authors.initials: C
authors.lastname: Orimoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishimukai
mesh_terms: D041321:Chromosomes, Human, X; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
chemical_list: D011944:Receptors, Androgen