[The spectroscopic studies on the binding of Al(III) to EHPG].
Interaction of ethylene-N,N'-bis(o-hydioxyphenylglycine) (EHPG) with Al3+ has been investigated by both UV difference and fluorescent spectra. Both results show that the molar ration of the complex is most likely 1:1. Aluminum binding produces peaks at 235 and 291 nm. The molar absorptivity of aluminum ions to EHPG at 235 nm is 1.27 x 10(4) cm-1.mol-1.L. The conditional stability constant for Al3+ binding to EHPG is determined to be IgK = 14.20 +/- 0.03 in 0.1 mol.L-1 Hepes buffer at room temperature, pH 7.4 by UV difference spectra. At the same condition, the fluorescent intensity of EHPG at 310 nm has been monitored. In result, the fluorescent intensity of EHPG at 310 nm is decreased with the addition of Al3+. Then the quench of the fluorescent intensity is ascribed to deprotonated phenolic groups coordinated to aluminum ions.
Li
Ying-qi
YQ
Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Bai
Hai-jing
HJ
Yang
Bin-sheng
BS
D000535:Aluminum / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D002614:Chelating Agents; D005029:Ethylenediamines; C011813:ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid); D000535:Aluminum