title: Myoendothelial differentiation of human umbilical cord blood-derived stem cells in ischemic limb tissues.
abstract: Human umbilical cord blood (UCB) contains high numbers of endothelial progenitors cells (EPCs) characterized by coexpression of CD34 and CD133 markers. Prior studies have shown that CD34+/CD133+ EPCs from the cord or peripheral blood (PB) can give rise to endothelial cells and induce angiogenesis in ischemic tissues. In the present study, it is shown that freshly isolated human cord blood CD34+ cells injected into ischemic adductor muscles gave rise to endothelial and, unexpectedly, to skeletal muscle cells in mice. In fact, the treated limbs exhibited enhanced arteriole length density and regenerating muscle fiber density. Under similar experimental conditions, CD34- cells did not enhance the formation of new arterioles and regenerating muscle fibers. In nonischemic limbs CD34+ cells increased arteriole length density but did not promote formation of new muscle fibers. Endothelial and myogenic differentiation ability was maintained in CD34+ cells after ex vivo expansion. Myogenic conversion of human cord blood CD34+ cells was also observed in vitro by coculture onto mouse myoblasts. These results show that human cord blood CD34+ cells differentiate into endothelial and skeletal muscle cells, thus providing an indication of human EPCs plasticity. The full text of this article is available online at http://www.circresaha.org.
authors.affiliation: Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Monzino, IRCCS, Milan, Italy. m.pesce@idi.it
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pesce
authors.forename: Alessia
authors.initials: A
authors.lastname: Orlandi
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Iachininoto
authors.forename: Stefania
authors.initials: S
authors.lastname: Straino
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Torella
authors.forename: Vania
authors.initials: V
authors.lastname: Rizzuti
authors.forename: Giulio
authors.initials: G
authors.lastname: Pompilio
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Bonanno
authors.forename: Giovanni
authors.initials: G
authors.lastname: Scambia
authors.forename: Maurizio C
authors.initials: MC
authors.lastname: Capogrossi
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006614:Hindlimb / Q000098:blood supply*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007511:Ischemia / Q000628:therapy; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D012038:Regeneration; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34