title: [Immortalization of human osteoblast by transferring hTERT gene].
abstract: OBJECTIVE
  To establish an immortalized osteoblast cell line used for basal and clinical research of orthopaedics.
  
  
  METHODS
  Human marrow stromal cells (hMSCs) had been inducted into osteoblasts directionally, and human telomerase reverse transcriptase (hTERT) cDNA was transferred into the osteoblasts by retroviral vector pLPChTERT. The resultant stable clones reproduced successively and the expression of hTERT as well as the osteogenesis characteristics of different eras were identified.
  
  
  RESULTS
  hTERT gene has been transferred into human osteoblasts successfully. The transformed cells expressed telomerase activity and divided vigorously. p62 has been obtained so far. The expression of bone specific Alkaline phosphatase, Collagen type I and Osteopontin of p25, p55 were checked and it is proved that the immortalized cell line preserved typical osteogenesis characteristics.
  
  
  CONCLUSIONS
  Osteoblasts can be immortalized by transferring exogenous hTERT gene to reconstitute telomerase activity, and the immortalized cell reserved osteogenesis characteristics.
authors.affiliation: Department of Orthopaedics, Third Hospital, Peking University, Beijing 100083, China.
authors.forename: Xiao-xue
authors.initials: XX
authors.lastname: Yin
authors.forename: Zhong-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Zhao-qing
authors.initials: ZQ
authors.lastname: Guo
authors.forename: Geng-ding
authors.initials: GD
authors.lastname: Dang
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Ma
authors.forename: Shen-wu
authors.initials: SW
authors.lastname: Wang
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D019098:Telomerase