Molecular cytogenetic approach to the diagnosis of splenic lymphoma: a case report of blastoid mantle cell lymphoma.
A 69-year-old woman was admitted to hospital due to abdominal pain. An enlarged spleen was detected and extirpated. Histological examination and FACS analysis could not clearly differentiate between a splenic marginal zone lymphoma and a mantle cell lymphoma. Using molecular cytogenetic techniques, a complex karyotype including a translocation between chromosome 11 and 14 consistent with a triple fusion between heavy chain immunoglobulin locus (IgH) and cyclin D1 (CCND1) was demonstrated. Overexpression of cyclin D1 was detected by immunostaining. Thus the diagnosis of a splenic blastoid mantle cell lymphoma could be established. In this report, various aspects of differential diagnosis of splenic lymphomas are discussed.
Björck
Erik
E
Department of Molecular Medicine, CMM L8:02 Karolinska Hospital and Institutet, SE-171 76, Stockholm, Sweden. erik.bjorck@cmm.ki.se
Landgren
Ola
O
Schoumans
Jacqueline
J
Christensson
Birger
B
Björkholm
Magnus
M
MacDonald
Anna Porwit
AP
Nordenskjöld
Magnus
M
D000368:Aged; D002478:Cells, Cultured; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D019938:Cyclin D1 / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D013160:Splenic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D014178:Translocation, Genetic
D019938:Cyclin D1