title: Effect of quinones and phenols on the triple-enzyme bioluminescent system with protease.
abstract: The study addressed the effects of redox-active compounds on trypsin activity. Series of organic oxidizers (quinones) and reducers (phenols) were chosen as model redox-active compounds. Trypsin activity was quantified by bioluminescent technique. Interactions of these compounds with trypsin were studied by fluorescent and light absorption methods. Luminescence intensity decay constants in the reduced nicotinamidadeninedinucleotide (NADH): flavinmononucleotide (FMN)-oxidoreductase (R)-luciferase (L)-trypsin (T) (R + L + T) triple-enzyme system were calculated and compared in the presence of different concentrations of quinones and phenols. The triple-enzyme system was shown to be sensitive to quinones and not sensitive to phenols. It has been found that the effects produced by quinones on the coupled enzyme system (R + L) and on the trypsin molecule (T) are not related. The conclusions were extrapolated to the properties of other proteases and antiproteases.
authors.affiliation: Institute of Biophysics, Akademgorodok, 660036 Krasnoyarsk, Russia.
authors.forename: N S
authors.initials: NS
authors.lastname: Kudryasheva
authors.forename: E N
authors.initials: EN
authors.lastname: Esimbekova
authors.forename: N N
authors.initials: NN
authors.lastname: Remmel
authors.forename: V A
authors.initials: VA
authors.lastname: Kratasyuk
authors.forename: A J W G
authors.initials: AJ
authors.lastname: Visser
authors.forename: A
authors.initials: A
authors.lastname: van Hoek
mesh_terms: D010450:Endopeptidases / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D038181:FMN Reductase / Q000378:metabolism; D005454:Fluorescence Polarization; D008156:Luciferases / Q000378:metabolism; D049449:Luminescence; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis*; D011809:Quinones / Q000032:analysis*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010636:Phenols; D011809:Quinones; D008156:Luciferases; D038181:FMN Reductase; D010450:Endopeptidases; D014357:Trypsin