title: The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III.
abstract: Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase II from RNA polymerase III with good yield.
authors.affiliation: Department of Biochemistry and Biotechnology, Agricultural University, Wołyńska 35, 60-637 Poznań, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Glanc
authors.forename: Mariola
authors.initials: M
authors.lastname: Galbas
authors.forename: Piotr
authors.initials: P
authors.lastname: Dullin
authors.forename: Marlena
authors.initials: M
authors.lastname: Szalata
authors.forename: Ryszard
authors.initials: R
authors.lastname: Słomski
mesh_terms: D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D012319:RNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism; D012320:RNA Polymerase III / Q000302:isolation & purification* / Q000378:metabolism; D014908:Triticum / Q000201:enzymology*
chemical_list: D012319:RNA Polymerase II; D012320:RNA Polymerase III