title: EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human.
abstract: A molecular understanding of porcine reproduction is of biological interest and economic importance. Our Midwest Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited into public databases 21,499 expressed sequence tag (EST) gene sequences from the 3' end of clones from these libraries. These sequences represent 10,574 different genes, based on sequence comparison among these data, and comparison with existing porcine ESTs and genes indicate as many as 4652 of these EST clusters are novel. In silico analysis identified sequences that are expressed in specific pig tissues or organs and confirmed the broad expression in pig for many genes ubiquitously expressed in human tissues. Furthermore, we have developed computer software to identify sequence similarity of these pig genes with their human counterparts, and to extract the mapping information of these human homologues from genome databases. We demonstrate the utility of this software for comparative mapping by localizing 61 genes on the porcine physical map for Chromosomes (Chrs) 5, 10, and 14.
authors.affiliation: Center for Integrated Animal Genomics and Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, Iowa 50011, USA. cktuggle@iastate.edu
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Tuggle
authors.forename: Jon A
authors.initials: JA
authors.lastname: Green
authors.forename: Carolyn
authors.initials: C
authors.lastname: Fitzsimmons
authors.forename: Rami
authors.initials: R
authors.lastname: Woods
authors.forename: Randall S
authors.initials: RS
authors.lastname: Prather
authors.forename: Sergei
authors.initials: S
authors.lastname: Malchenko
authors.forename: Bento M
authors.initials: BM
authors.lastname: Soares
authors.forename: Tamara
authors.initials: T
authors.lastname: Kucaba
authors.forename: Keith
authors.initials: K
authors.lastname: Crouch
authors.forename: Christina
authors.initials: C
authors.lastname: Smith
authors.forename: Dylan
authors.initials: D
authors.lastname: Tack
authors.forename: Natalie
authors.initials: N
authors.lastname: Robinson
authors.forename: Brian
authors.initials: B
authors.lastname: O'Leary
authors.forename: Todd
authors.initials: T
authors.lastname: Scheetz
authors.forename: Thomas
authors.initials: T
authors.lastname: Casavant
authors.forename: Daniel
authors.initials: D
authors.lastname: Pomp
authors.forename: Brad J
authors.initials: BJ
authors.lastname: Edeal
authors.forename: Yuandan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Max F
authors.initials: MF
authors.lastname: Rothschild
authors.forename: Kevin
authors.initials: K
authors.lastname: Garwood
authors.forename: William
authors.initials: W
authors.lastname: Beavis
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*