title: Evaluation of shotgun sequencing for proteomic analysis of human plasma using HPLC coupled with either ion trap or Fourier transform mass spectrometry.
abstract: This paper reports on studies directed to the characterization of the proteome of human plasma by the shotgun sequencing approach, namely the use of HPLC coupled to mass spectrometry (MS). The report will present data from two laboratories that allows the comparison of peptide and protein identifications by either accurate mass measurement on a Fourier transform mass spectrometry or MS/MS fragmentation on an ion trap mass spectrometer. Because the dynamic range of the protein components of plasma is one of the largest for a biological sample, the analysis of such a challenging sample was aided by the use of these two MS approaches. The major classes of proteins observed were transport proteins, enzymes, and enzyme inhibitors, blood-clotting factors, membrane-associated proteins including soluble forms of receptors, hormones, immunoglobulins, and other glycoproteins. The protein identifications were also highly consistent with results obtained from 2D gel studies, although a larger number of additional proteins were observed with the shotgun sequencing approach. The quantitation of low to medium level proteins was explored in the ion trap with an add-back of a known amount of human growth hormone (hGH) at a clinically relevant level (5 ug/L). The isotope coded affinity tag (ICAT) approach was used to quantitate successfully different levels of hGH in replicate analysis via the disulfide linked tryptic peptide (T6-T16). These studies suggest that the shotgun sequencing approach can be used to characterize part of the plasma proteome and serve as a starting point for the use of multidimensional analytical approaches for the analysis of complex biological samples.
authors.affiliation: ThermoFinnigan, 355 River Oaks Parkway, San Jose, California, USA.
authors.forename: Shiaw-Lin
authors.initials: SL
authors.lastname: Wu
authors.forename: Gargi
authors.initials: G
authors.lastname: Choudhary
authors.forename: Margareta
authors.initials: M
authors.lastname: Ramström
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
authors.forename: William S
authors.initials: WS
authors.lastname: Hancock
mesh_terms: D000465:Algorithms; D001798:Blood Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019295:Computational Biology; D030562:Databases, Protein; D001330:Electronic Data Processing; D015180:Electrophoresis, Gel, Two-Dimensional; D005583:Fourier Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D019382:Human Growth Hormone / Q000032:analysis; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D001798:Blood Proteins; D010446:Peptide Fragments; D019382:Human Growth Hormone