Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize.
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
Masarik
Michal
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic.
Kizek
Rene
R
Kramer
Karl J
KJ
Billova
Sabina
S
Brazdova
Marie
M
Vacek
Jan
J
Bailey
Michele
M
Jelen
Frantisek
F
Howard
John A
JA
D000327:Adsorption; D001360:Avidin / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D001710:Biotin / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D009693:Nucleic Acid Hybridization / Q000379:methods; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D019809:Streptavidin / Q000032:analysis / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics*
D001360:Avidin; D001710:Biotin; D004247:DNA; D019809:Streptavidin