Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein.
Pichia pastoris secretes few native proteins. However, the more than 1 g l(-1)of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A(13)C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.
Palczewska
Małgorzata
M
Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
Batta
Gyula
G
Groves
Patrick
P
D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000302:isolation & purification*; D010843:Pichia / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D011994:Recombinant Proteins / Q000378:metabolism*; D012685:Sepharose / Q000378:metabolism*
D002247:Carbon Isotopes; D008351:Mannans; D010940:Plant Proteins; D011134:Polysaccharides; D011994:Recombinant Proteins; D003208:Concanavalin A; D012685:Sepharose