[Effect of low-power laser irradiation on the structure and properties of protein molecules under non-specific energy absorbance].
The influence of the helium-neon laser emission on HSA was investigated at present work by means of the fluorescent probe MNBIS (4-morpholino-7-oxy-7-H-benzo-[de]-benzo-[4,5]-imidazo-[2,1-a]- izoquinolin-5-sulfonic acid) sensitive to protein conformational changes using. Fluorescence spectra parameters of the probe and kinetics of probe interaction with protein molecules were analyzed. Possible mechanisms of the helium-neon laser irradiation on water-protein solution structure are discussed.
Romodanova
E O
EO
Laser Biology and Laser Medicine Research Iinstitute, V.N. Karazin Kharkov National University.
Diubko
T S
TS
Morozova
T F
TF
Roshal'
O D
OD
Distanov
V B
VB
Hurkalenko
Iu O
IuO
D005456:Fluorescent Dyes / Q000737:chemistry / Q000528:radiation effects; D006371:Helium; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D015394:Molecular Structure; D009356:Neon; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D012709:Serum Albumin / Q000737:chemistry / Q000528:radiation effects; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D014867:Water; D006371:Helium; D014443:Tyrosine; D009356:Neon; D014364:Tryptophan