Determination of HBsAg subtypes in Western Siberian part of Russia.
A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2:8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg-positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2:58 (15 ayw3varA and 43 ayw3varB; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3:(both variants, 33 ayw3varA and 1 ayw3varB; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk.
Netesova
I G
IG
Vector-Best, Inc., Koltsovo, Novosibirsk oblast, Russia. sijk@online.sinor.ru
Swenson
P D
PD
Osipova
L P
LP
Gubina
M A
MA
Posukh
O L
OL
Netesov
S V
SV
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001782:Blood Donors; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood* / Q000145:classification* / Q000276:immunology; D006515:Hepatitis B virus / Q000145:classification / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D015995:Prevalence; D011933:Reagent Kits, Diagnostic; D012800:Siberia / Q000453:epidemiology; D015819:Substance Abuse, Intravenous / Q000150:complications
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic