title: Multicenter evaluation of the fully automated bactec MGIT 960 system and three molecular methods for the isolation and the identification of mycobacteria from clinical specimens.
abstract: A combination of Bactec MGIT 960 system, a PCR-based assay and a PCR-Restriction Analysis procedure (PRA) was assessed for the detection and identification of mycobacteria from clinical samples. The MGIT recovered 243 from 266 mycobacterial isolates. The PCR-based assay correctly identified all (234) Mycobacterium tuberculosis isolates, while 35 nontuberculous mycobacterial isolates were identified by PRA.
authors.affiliation: Department of Microbiology, Medical School, University Hospital of Larissa, Papakyriazi 22, Larissa, Greece.
authors.forename: F
authors.initials: F
authors.lastname: Kontos
authors.forename: E
authors.initials: E
authors.lastname: Petinaki
authors.forename: S
authors.initials: S
authors.lastname: Nicolaou
authors.forename: Z
authors.initials: Z
authors.lastname: Gitti
authors.forename: S
authors.initials: S
authors.lastname: Anagnostou
authors.forename: M
authors.initials: M
authors.lastname: Maniati
authors.forename: C
authors.initials: C
authors.lastname: Costopoulos
authors.forename: I
authors.initials: I
authors.lastname: Tselentis
authors.forename: A N
authors.initials: AN
authors.lastname: Maniatis
mesh_terms: D001331:Automation; D015373:Bacterial Typing Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic