Purification, crystallization and preliminary X-ray crystallographic analysis of nucleoside diphosphate kinase from rice.
Nucleoside diphosphate kinase (NDK) catalyses the transfer of the gamma-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP). NDK is involved in and required for coleoptile elongation in rice. The level of the enzyme changes during seed germination and the early stages of seedling growth. Recombinant rice NDK (rNDK) has been crystallized using the hanging-drop vapour-diffusion method. rNDK crystals diffracted to a resolution of 2.50 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.98, b = 182.26, c = 188.30 A. Preliminary analysis indicates there to be 12 rNDK molecules in each asymmetric unit, with a solvent content of 47.2%.
Huang
Jen-Yen
JY
X-ray Structural Biology Group, National Synchrotron Radiation Research Center, Hsinchu 30077, Taiwan, and Department of Life Sciences, National Tsing-Hua University, Hsinchu 30077, Taiwan.
Chang
Chia-Yu
CY
Chang
Tschining
T
Chen
Chun-Jung
CJ
D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation
D010940:Plant Proteins; D009701:Nucleoside-Diphosphate Kinase