Expression of class I MHC molecule, HSP70 and TAP in human hepatocellular carcinoma.
OBJECTIVE
To demonstrate whether class I MHC molecule, transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine against hepatocellular carcinoma (HCC).


METHODS
We studied 30 HCC specimens by labeled streptavidin biotin (LSAB) method of immunohistochemistry.


RESULTS
The results showed that the majority of HCC cells investigated naturally expressed class I MHC and TAP, which were different from other tumor cells. Furthermore, we found that HSP70 expressed not only in cellular cytoplasm, but also on the cell surface in HCC.


CONCLUSIONS
Our findings indicate that our understanding about immune escape mechanisms employed by HCC cells may be further improved. It is important to design and construct CTL vaccine against HCC.
Deng
Xiao-Ling
XL
Immunology Department, West China Medical Center of Sichuan University, Chengdu 610044, Sichuan Province, China.
Chen
Wei
W
Cai
Mei-Ying
MY
Wei
Da-Peng
DP
D018528:ATP-Binding Cassette Transporters; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D018840:HSP70 Heat-Shock Proteins; D015395:Histocompatibility Antigens Class I; C477327:transporter associated with antigen processing (TAP)