title: [Study on the purification of SCAMP and the preparation of its sulfated polysaccharides].
abstract: Shark cartilage acid mucopolysaccharide (SCAMP) was extracted from cartilage by the improved method, then we can get SCAMP-F1 and SCAMP-F2 from SCAMP by sepharose-4B chromatography. It was improved that SCAMP-F2 had only one component by sepharose-4B chromatography, PAGE (polyacrylamide gel electrophoresis) and especial centrifuge. At the same time, SCAMP-F2 reacted with sulfated reagents (composed of chlorosulfonic acid and pyridine), and sulfated SCAMP-F2 was obtained. Its sulfur content is 19.6%, higher 6.1% than unsulfated SCAMP-F2, IR and 13C-NMR data showed that additional sulfates were located on the carbon-6.
authors.affiliation: Life Science College of Nanjing Normal University, Nanjing 210097, China.
authors.forename: Dong-xia
authors.initials: DX
authors.lastname: Li
authors.forename: Shuang-quan
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012754:Sharks; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D013431:Sulfates