title: Sialic acid 9-O-acetylesterase catalyzes the hydrolyzing reaction from alacepril to deacetylalacepril.
abstract: OBJECTIVE
  In this work, the alacepril thiolesterase, which catalyzes the hydrolyzing reaction of the thiolester linkage in alacepril and the conversion from alacepril to deacetylalacepril, was purified from rat liver cytosol and characterized.
  
  
  METHODS
  A purification procedure for the thiolesterase consisted of ammonium sulfate fractionation and chromatographies with phenyl Sepharose CL-4B, Q Sepharose FF, ceramic hydroxylapatite, and phenyl Sepharose HP. The thiolesterase activity was assayed for alacepril as a substrate and the reaction product, deacetylalacepril, was measured using high-performance liquid chromatography.
  
  
  RESULTS
  The purified thiolesterase is heterodimeric with a molecular mass of 29 and 36 kDa subunits as estimated by sodium dodecyl sulfate -polyacrylamide gel electrophoresis. N-terminal amino acid sequence of these subunits reveals that the thiolesterase is identical to sialic acid 9-O-acetylesterase. The thiolesterase hydrolyzes not only the thiolester bond in alacepril, spironolactone, and acetyl coenzyme A but also the carboxylester bond in alpha-naphtyl acetate. The alacepril thiolestrase activity is competitively inhibited by alpha-naphtyl acetate.
  
  
  CONCLUSIONS
  The thiolesterase, i.e., sialic acid 9-O-acetylesterase, seems to be involved in the metabolism of certain drugs such as alacepril and spironolactone. However, drugs having ester-type and amide-type linkages, for example dilazep. aniracetam, and benazepril, are not substrates for the thiolestrase.
authors.affiliation: Department of Pharmaceutics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Usui
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kubota
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iguchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kiho
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katagiri
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hirano
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000959:Antihypertensive Agents / Q000378:metabolism; D002216:Captopril / Q000031:analogs & derivatives* / Q000378:metabolism*; D002853:Chromatography, Liquid; D006868:Hydrolysis; D008099:Liver / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D000959:Antihypertensive Agents; C048162:desacetyl-alacepril; D002216:Captopril; D000123:Acetyltransferases; C099970:sialic acid O-acetyltransferase; C046835:alacepril