Determination of trace RA by capillary electrophoresis-solid-phase microextraction with direct UV detection.
A new method of offline solid-phase microextraction coupled with capillary electrophoresis (CE) [in an uncoated capillary using borax and boric acid in acetonitrile-water (40:60) as the running buffer] with UV detection at 254 nm is presented and used for direct determination of trace biomedically important retinoic acid (RA) without any derivation. As low as 138 ng/mL RA in aqueous solution can be determined using CE with UV detection with a concentration factor of 19.6 times.
Fang
Mei
M
National Chromatographic R&A Center of China, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian, 116011, PR China. mfangcd@yahoo.com
Xu
Guowang
G
D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014212:Tretinoin / Q000032:analysis*
D014212:Tretinoin