[Suggestive evidences for a microanatomical relationship between mast cells and nerve in the tongue of the toad Bufo marinus demonstrated by means of high resolution light microscopy].
Mast cells are connective tissue cells, present in all vertebrates and characterized by the metachromatic stain of their granules. Nowadays mast cells have been recognized as a potent cellular source of multiple cytokines, suggesting an important role in immunoregulation and host defense. These cells have been described as preferentially located around blood vessels but more recently close spatial relationship between mast cells and nerves has been reported mostly in mammalian species. The microanatomy of nerve tissue and associated mast cells in the toad Bufo marinus tongue have been studied here by means of high resolution light microscopy. Mast cell population was identified by the metachromatic staining of their cytoplasm granules in Epon embedded semithin sections counterstained with Toluidine Blue and Azure A. Numerous mast cells were observed scattered throughout the submucosal region, adjacent and/or within of nerve bundles and nerve ganglia, near skeletal muscle fibers and adjacent of blood vessels. Additionally, mast cells adjacent to single but conspicuous myelinated nerve fibers were seen under endothelia of lymphatic vessels wall and this is apparently a unreported event. Results suggest nerve-mast cell associations are functionally important in the toad tongue.
González-Elorriaga
Miren Alatzne
MA
Facultad de Ciencias, Universidad Central de Venezuela, Caracas. mgonzale@electra.ciens.ucv.ve
Canepa
Gloria
G
D000818:Animals; D002024:Bufo marinus / Q000033:anatomy & histology*; D008036:Lingual Nerve / Q000648:ultrastructure; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure*; D009417:Nerve Tissue / Q000648:ultrastructure*; D014059:Tongue / Q000166:cytology* / Q000294:innervation* / Q000648:ultrastructure