title: Elimination of Candida albicans infection of the radicular dentin by intracanal medications.
abstract: Fungi have been associated with cases of secondary or persistent root canal infections. The purpose of this study was to evaluate the effectiveness of four intracanal medications in disinfecting the root dentin of bovine teeth experimentally infected with Candida albicans. Infected dentin cylinders were exposed to four different medications: calcium hydroxide/glycerin; calcium hydroxide/0.12% chlorhexidine digluconate; calcium hydroxide/camphorated paramonochlorophenol/glycerin; and 0.12% chlorhexidine digluconate/zinc oxide. Specimens were in contact with the medications for 1 h, 2 days, and 7 days. The viability of C. albicans after exposure was evaluated by specimen incubation in culture medium to compare the effectiveness of the medications in disinfecting dentin. Results showed that the specimens treated with calcium hydroxide/camphorated paramonochlorophenol/ glycerin paste or with chlorhexidine/zinc oxide paste were completely disinfected after 1 h of exposure. Calcium hydroxide/glycerin paste only consistently eliminated C. albicans infection after 7 days of exposure. Calcium hydroxide mixed with chlorhexidine was ineffective in disinfecting dentin even after 1 week of medication exposure. Among the medications tested, the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste and chlorhexidine digluconate mixed with zinc oxide were the most effective in eliminating C. albicans cells from dentinal specimens.
authors.affiliation: Department of Endodontics, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
authors.forename: Hélio P
authors.initials: HP
authors.lastname: Lopes
authors.forename: Fernando A C
authors.initials: FA
authors.lastname: Magalhães
authors.forename: Milton
authors.initials: M
authors.lastname: de Uzeda
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002164:Camphor / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D003804:Dentin / Q000382:microbiology*; D004338:Drug Combinations; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013997:Time Factors; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D002733:Chlorophenols; D004338:Drug Combinations; D012388:Root Canal Irrigants; D002164:Camphor; C029804:camphorated parachlorophenol; D002126:Calcium Hydroxide; D002710:Chlorhexidine; D015034:Zinc Oxide