title: Calculating order parameter profiles utilizing magnetically aligned phospholipid bilayers for 2H solid-state NMR studies.
abstract: Solid-state deuterium NMR spectroscopy was used to study the structural and dynamic properties of stearic acid-d(35) in magnetically aligned phospholipid bilayers as a function of temperature. Magnetically aligned phospholipid bilayers or bicelles are model systems, which mimic biological membranes for magnetic resonance studies. Paramagnetic lanthanide ions (Yb(3+)) were added to align the bicelles such that the bilayer normal is colinear with the direction of the static magnetic field. The corresponding order parameters of the stearic acid-d(35) probe were calculated and compared with values obtained from unoriented samples in the literature. The addition of cholesterol to the bicelle system decreases the fluidity of the phospholipid bilayers and increases the ordering of the acyl chains of stearic acid-d(35). This study demonstrates the feasibility of utilizing magnetically aligned bicelles for calculating 2H order parameter profiles for non-biological systems such as polymer-grafted membranes and Schiff's base complexes.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
authors.forename: Paresh C
authors.initials: PC
authors.lastname: Dave
authors.forename: Elvis K
authors.initials: EK
authors.lastname: Tiburu
authors.forename: Nisreen A
authors.initials: NA
authors.lastname: Nusair
authors.forename: Gary A
authors.initials: GA
authors.lastname: Lorigan
mesh_terms: D000465:Algorithms; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000528:radiation effects; D004574:Electromagnetic Fields; D008051:Lipid Bilayers / Q000737:chemistry* / Q000528:radiation effects*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008560:Membrane Fluidity / Q000528:radiation effects*; D008968:Molecular Conformation; D009038:Motion; D010742:Phospholipid Ethers / Q000737:chemistry / Q000528:radiation effects; D010743:Phospholipids / Q000737:chemistry* / Q000528:radiation effects*; D013229:Stearic Acids / Q000737:chemistry / Q000528:radiation effects; D013696:Temperature; D015018:Ytterbium / Q000737:chemistry
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D008051:Lipid Bilayers; D010742:Phospholipid Ethers; D010743:Phospholipids; D013229:Stearic Acids; C031183:stearic acid; D015018:Ytterbium; D004134:Dimyristoylphosphatidylcholine