title: Effect of microbial phytase on energy availability, and lipid and protein deposition in growing swine.
abstract: Two experiments were conducted to determine the effect of phytase on energy availability in pigs. In Exp. 1, barrows (initial and final BW of 26 and 52 kg) were allotted to four treatments in a 2 x 2 factorial arrangement. Corn-soybean meal (C-SBM) diets were fed at two energy levels (2.9 and 3.2 x maintenance [M]) with and without the addition of 500 phytase units/kg of diet. The diets contained 115% of the requirement for Ca, available P (aP), and total lysine, and Ca and aP were decreased by 0.10% in diets with added phytase. Pigs were penned individually and fed daily at 0600 and 1700, and water was available constantly. Eight pigs were killed and ground to determine initial body composition. At the end of Exp. 1, all 48 pigs were killed for determination of carcass traits and protein and fat content by total-body electrical conductivity (TOBEC) analysis. Six pigs per treatment were ground for chemical composition. In Exp. 2, 64 barrows and gilts (initial and final BW of 23 and 47 kg) were allotted to two treatments (C-SBM with 10% defatted rice bran or that diet with reduced Ca and aP and 500 phytase units/kg of diet), with five replicate pens of barrows and three replicate pens of gilts (four pigs per pen). In Exp. 1, ADG was increased (P < 0.01) in pigs fed at 3.2 x M. Based on chemical analyses, fat deposition, kilograms of fat, retained energy (RE) in the carcass and in the carcass + viscera, fat deposition in the organs, and kilograms of protein in the carcass were increased (P < 0.10) in pigs fed the diets at 3.2 vs. 2.9 x M. Based on TOBEC analysis, fat deposition, percentage of fat increase, and RE were increased (P < 0.09) in pigs fed at 3.2 x M. Plasma urea N concentrations were increased in pigs fed at 3.2 x M with no added phytase but were not affected when phytase was added to the diet (phytase x energy, P < 0.06). Fasting plasma glucose measured on d 28, ultrasound longissimus muscle area (LMA), and 10th-rib fat depth were increased (P < 0.08) in pigs fed phytase, but many other response variables were numerically affected by phytase addition. In Exp. 2, phytase had no effect (P > 0.10) on ADG, ADFI, gain:feed, LMA, or 10th-rib fat depth. These results suggest that phytase had small, mostly nonsignificant effects on energy availability in diets for growing pigs; however, given that phytase increased most of the response variables measured, further research on its possible effects on energy availability seems warranted.
authors.affiliation: Department of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge 70803-4210, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Shelton
authors.forename: L L
authors.initials: LL
authors.lastname: Southern
authors.forename: T D
authors.initials: TD
authors.lastname: Bidner
authors.forename: M A
authors.initials: MA
authors.lastname: Persica
authors.forename: J
authors.initials: J
authors.lastname: Braun
authors.forename: B
authors.initials: B
authors.lastname: Cousins
authors.forename: F
authors.initials: F
authors.lastname: McKnight
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage* / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001806:Blood Urea Nitrogen; D001823:Body Composition / Q000187:drug effects* / Q000502:physiology; D004041:Dietary Fats / Q000378:metabolism*; D004044:Dietary Proteins / Q000378:metabolism*; D004553:Electric Conductivity; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D008297:Male; D009929:Organ Size; D011897:Random Allocation; D013552:Swine / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D004044:Dietary Proteins; D010832:6-Phytase