title: Disruption of a novel gene, DIRC3, and expression of DIRC3-HSPBAP1 fusion transcripts in a case of familial renal cell cancer and t(2;3)(q35;q21).
abstract: Previously, we identified a family with renal cell cancer and a t(2;3)(q35;q21). Positional cloning of the chromosome 3 breakpoint led to the identification of a novel gene, DIRC2, that spans this breakpoint. Here we have characterized the chromosome 2 breakpoint in detail and found that another novel gene, designated DIRC3, spans this breakpoint. In addition, we found that the first two exons of DIRC3 can splice to the second exon of HSPBAP1, a JmjC-Hsp27 domain gene that maps proximal to the breakpoint on chromosome 3. This splice results in the formation of DIRC3-HSPBAP1 fusion transcripts. We propose that these fusion transcripts may affect normal HSPBAP1 function and concomitant chromatin remodeling and/or stress response signals within t(2;3)(q35;q21)-positive kidney cells. As a consequence, familial renal cell cancer may develop.
authors.affiliation: Department of Human Genetics, University Medical Center Nijmegen, Nijmegen, The Netherlands. d.bodmer@antrg.azn.nl
authors.forename: Daniëlle
authors.initials: D
authors.lastname: Bodmer
authors.forename: Marga
authors.initials: M
authors.lastname: Schepens
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Eleveld
authors.forename: Eric F P M
authors.initials: EF
authors.lastname: Schoenmakers
authors.forename: Ad
authors.initials: A
authors.lastname: Geurts van Kessel
mesh_terms: D000328:Adult; D000818:Animals; D016466:CHO Cells; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D002461:Cell Line, Transformed; D019457:Chromosome Breakage / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D006224:Cricetinae; D006580:Genetic Carrier Screening; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis / Q000235:genetics*; D062085:RNA, Long Noncoding; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; C439384:DIRC1 lncRNA, human; C455829:HSPBAP1 protein, human; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D015514:Oncogene Proteins, Fusion; D062085:RNA, Long Noncoding