Comparison of media for optimal recovery of Candida albicans and Candida glabrata from blood culture.
BACKGROUND
Candida spp., mainly Candida albicans, are frequently responsible for complications in immunocompromised patients. There are limited data comparing recovery efficiency using simple non-selective basal broth media.


OBJECTIVE
To compare several commercially available basal growth media to determine the medium that gave highest yeast proliferation.


METHODS
Eight commercially available non-selective basal broth culture media were evaluated for optimum recovery of clinical C. albicans and C. glabrata. They included nutrient broth (NB), nutrient broth no. 2 (NB2), Todd-Hewitt (TH) broth, tryptone soya broth (TSB), tryptone soya broth supplemented with yeast extract (0.5% w/v [TSBYE]), brain heart infusion broth supplemented with yeast extract (0.5% w/v [BHIYE]), salt meat broth (SMB) and 0.1% [w/v] peptone saline (PS). Differences in cell density were evaluated by spectrophotometrical analysis.


RESULTS
TSBYE>BHIYE>TSB>TH>NB2>NB>SMB>PS for the optimum proliferation of cells in vitro. Either TSBYE or BHIYE broth may be employed as suitable basal broth media for growth of C. albicans and C. glabrata. NB should be considered the least suitable medium for routine use when others are available.


CONCLUSIONS
These data may be of value to laboratories setting up simple blood culture systems to detect Candida spp., particularly in developing and underdeveloped countries.
Moore
J E
JE
Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Northern Ireland. jemoore@niphl.dnet.co.uk
McMullan
R
R
D001769:Blood / Q000382:microbiology*; D002176:Candida albicans / Q000302:isolation & purification*; D041221:Candida glabrata / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans
D003470:Culture Media