Array-based analysis on tobacco plastid transcripts: preparation of a genomic microarray containing all genes and all intergenic regions.
The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.
Nakamura
Takahiro
T
Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya, 467-8501 Japan.
Furuhashi
Yumiko
Y
Hasegawa
Keiko
K
Hashimoto
Hiroshi
H
Watanabe
Kazufumi
K
Obokata
Junichi
J
Sugita
Mamoru
M
Sugiura
Masahiro
M
D018744:DNA, Plant; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018087:Plastids / Q000235:genetics*; D018749:RNA, Plant / Q000096:biosynthesis; D014026:Nicotiana / Q000235:genetics*
D018744:DNA, Plant; D018749:RNA, Plant