title: Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea.
abstract: We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).
authors.affiliation: Kumiai Chemical Industry Co Ltd, Life Science Research Institute, 3360 Kamo, Kikugawa, 439-0031 Japan. k-kaku@kumiai-chem.co.jp
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kaku
authors.forename: Makiichi
authors.initials: M
authors.lastname: Takagaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kozo
authors.initials: K
authors.lastname: Nagayama
mesh_terms: D000577:Amides / Q000494:pharmacology; D001483:Base Sequence; D003521:Cyclopropanes / Q000494:pharmacology; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D020082:Magnaporthe / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D008543:Melanins / Q000096:biosynthesis; D012275:Oryza / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: D000577:Amides; D003521:Cyclopropanes; D017931:DNA Primers; D008543:Melanins; D006836:Hydro-Lyases; C082741:scytalone dehydratase; C113565:carpropamid