Crystal structures of human DJ-1 and Escherichia coli Hsp31, which share an evolutionarily conserved domain.
Human DJ-1 and Escherichia coli Hsp31 belong to ThiJ/PfpI family, whose members contain a conserved domain. DJ-1 is associated with autosomal recessive early onset parkinsonism and Hsp31 is a molecular chaperone. Structural comparisons between DJ-1, Hsp31, and an Archaea protease, a member of ThiJ/PfpI family, lead to the identification of the chaperone activity of DJ-1 and the proteolytic activity of Hsp31. Moreover, the comparisons provide insights into how the functional diversity is realized in proteins that share an evolutionarily conserved domain. On the basis of the chaperone activity the possible role of DJ-1 in the pathogenesis of Parkinson's disease is discussed.
Lee
Sun-Joo
SJ
Beamline Division, Pohang Accelerator Laboratory, Pohang, 790-784, Kyungbuk, Republic of Korea.
Kim
So Jung
SJ
Kim
In-Kwon
IK
Ko
Junsang
J
Jeong
Chang-Sook
CS
Kim
Gyung-Hwa
GH
Park
Chankyu
C
Kang
Sa-Ouk
SO
Suh
Pann-Ghill
PG
Lee
Heung-Soo
HS
Cha
Sun-Shin
SS
D000595:Amino Acid Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003198:Computer Simulation; D017124:Conserved Sequence; D003460:Crystallization; D019281:Dimerization; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008156:Luciferases / Q000378:metabolism; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000235:genetics; D015513:Oncogene Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010300:Parkinson Disease / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics; D010447:Peptide Hydrolases / Q000378:metabolism; D017354:Point Mutation; D000071617:Protein Deglycase DJ-1; D020836:Protein Structure, Quaternary; D051843:Synucleins; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
D029968:Escherichia coli Proteins; D047908:Intracellular Signaling Peptides and Proteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D010446:Peptide Fragments; D051843:Synucleins; C469996:hchA protein, E coli; D008156:Luciferases; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D010447:Peptide Hydrolases