title: Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model.
abstract: We examined changes in mRNA expression patterns for proinflammatory cytokines and growth factors in blood samples after application of a tourniquet to the rat hind limb. Slight upregulations of interferon (IFN)-gamma, macrophage colony-stimulating factor (M-CSF) and transforming growth factor (TGF)-beta1 mRNA began at 2h after tourniquet application and were short-lived. The levels of activating transcription factor (ATF)-3, a stress-inducible gene, had increased at 1h after tourniquet application. No significant expression of interleukin (IL)-6 mRNA was observed in most samples. There were no significant temporal changes in the levels of IL-1beta, cardiotrophin (CT)-1 mRNA compared to the control levels, but, downregulation of gp130, a receptor of the IL-6 family, began at 1h after tourniquet application. Nerve growth factor (NGF) mRNA gradually increased and reached a significantly high level at 4h after application of the tourniquet. Gene expression induction in blood leukocytes occurred soon after application of the tourniquet and was short-lived. The transient mRNA expressions probably trigger secondary events that may be beneficial to wound repair and regeneration.
authors.affiliation: Department of Legal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. sumiko@fmu.ac.jp
authors.forename: Sumiko
authors.initials: S
authors.lastname: Abe
authors.forename: Ikubumi
authors.initials: I
authors.lastname: Mizusawa
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Kanno
authors.forename: Atsuko
authors.initials: A
authors.lastname: Yabashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Honda
authors.forename: Miwako
authors.initials: M
authors.lastname: Suto
authors.forename: Mikio
authors.initials: M
authors.lastname: Kuraya
authors.forename: Kouichi
authors.initials: K
authors.lastname: Hiraiwa
mesh_terms: D051700:Activating Transcription Factor 3; D000818:Animals; D015870:Gene Expression; D006614:Hindlimb; D007371:Interferon-gamma / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology; D009414:Nerve Growth Factors / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1
chemical_list: D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma