HPLC determination of aminophylline, methoxyphenamine hydrochloride, noscapine and chlorphenamine maleate in compound dosage forms with an aqueous-organic mobile phase.
A high-performance liquid chromatography procedure for the simultaneous determination of aminophylline, methoxyphenamine hydrochloride, noscapine and chlorphenamine maleate in commercially available compound capsule dosage forms has been developed and validated. The separation and quantification were achieved on an Ultrasphere C18 column using a mobile phase of dichloromethane-methanol-0.25% (v/v) diethylamine aqueous solution (20:60:20, v/v/v) at a flow rate of 1 ml min(-1) with detection of all analytes at 264 nm. The separation was achieved within 6 min for each drug mixture. The method showed good linearity for the aminophylline, noscapine, chlorphenamine maleate and methoxyphenamine hydrochloride mixture in the 125-750, 35-210, 10-60 and 62.5-375 microg ml(-1) ranges, respectively. The intra- and inter-day R.S.D.s ranged from 0.4 to 0.5%, 0.4-0.6%, 0.5-0.7% and 0.4-0.6% for aminophylline, noscapine, chlorphenamine maleate and methoxyphenamine hydrochloride, respectively. The recoveries (mean+/-S.D.) of low, middle and high concentrations were 99.9+/-0.9, 100.4+/-1.3 and 99.7+/-0.7% for aminophylline; 99.9+/-1.1, 100.4+/-0.7 and 100.1+/-0.8% for noscapine; 99.8+/-1.1, 99.7+/-1.0 and 100.7+/-0.8% for chlorphenamine maleate; and 99.8+/-0.9, 100.4+/-1.6 and 99.9+/-0.9% for methoxyphenamine hydrochloride, respectively.
Yin
Chunhua
C
State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China. chyin@fudan.edu.cn
Tang
Cui
C
Wu
Xiaoying
X
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000628:Aminophylline / Q000032:analysis*; D000996:Antitussive Agents / Q000032:analysis*; D001993:Bronchodilator Agents / Q000032:analysis*; D002214:Capsules; D002744:Chlorpheniramine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000032:analysis*; D009665:Noscapine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000318:Adrenergic beta-Agonists; D000996:Antitussive Agents; D001993:Bronchodilator Agents; D002214:Capsules; D004338:Drug Combinations; D007202:Indicators and Reagents; D012996:Solutions; D000628:Aminophylline; D002744:Chlorpheniramine; D008694:Methamphetamine; D009665:Noscapine; C005157:methoxyphenamine