[Expression of alpha-SMA mRNA in the lung tissue of rat with asthma].
OBJECTIVE
To investigate the expression of smooth muscle actin(SMA)-alpha mRNA in the lung of rat with asthma.


METHODS
A rat model of asthma was established by exposing the animal to aerosolized ovalbumin (OA). After being sensitized, the rat was subjected to laboratory tests. alpha-SMA mRNA of the lung tissue was measured by RT-PCR. The thickness of the airway smooth muscle(ASM) was measured by an image analysis system at the same time.


RESULTS
The thickness of ASM increased 2 weeks after OA challenge. The expression of alpha-SMA mRNA increased 1 week after OA challenge and increased more significantly after 2 weeks.


CONCLUSIONS
In the rat model of asthma, hypertrophy and hyperplasia of the airway smooth muscle were noted, and the phenotype was changed.
Wu
Zhaoping
Z
Department of Respiratory Disease, 452 Hospital of People's Liberation Army, Chengdu 610041, China.
Luo
Fengming
F
Wang
Zengli
Z
Liu
Xiaojing
X
Liu
Chuntao
C
Wang
Wenzhi
W
Zhang
Xiaohong
X
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism* / Q000473:pathology; D010047:Ovalbumin; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats
D000199:Actins; D012333:RNA, Messenger; D010047:Ovalbumin