[The expression of mRNA and SDS-PAGE of L1210 cell strains and its cloned cells].
OBJECTIVE
To examine the expression difference of mRNA of L1210 cell strains and its cloned cells and discuss the methods for quality control of cell strains.


METHODS
We used SDS-PAGE to observe the difference of protein and performed in situ hybridization to examine the expression of mRNA with the use of 6 cDNA probes that were marked by biotin.


RESULTS
The number of protein bands of L1210 from Beijing Cancer Institute was 32. The number of protein bands of the two cloned cells L3E11 and L3F9 was 31. The 6 cDNA probes (p16, c-fos, c-jun, c-myc, p21, and p53 mRNA) were found to be existing in Beijing Cancer Institute L1210 and two different cloned cell strains. Expression of c-myc, c-fos, p53 mRNA could distinguish L3E11 and L3F9 cloned cells.
Liu
Qiuyan
Q
Department of Laboratory Animal, Hebei Medical University, Shijiazhuang 050017, China.
Lu
Zhanjun
Z
Wang
Xiufang
X
Liu
Fuying
F
D000818:Animals; D002999:Clone Cells; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D017403:In Situ Hybridization; D007939:Leukemia L1210 / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53