title: The production of lipids alternately labelled with carbon-13.
abstract: Chlorella cells were shown to have similar fatty acid profiles when grown photoautotrophically or if grown photoheterotrophically with ethanoate (acetate) as carbon source. When supplied with ethanoate labelled with carbon-13 in the methyl group, the alga incorporated it into fatty acids with retention of the sequence of labelling on alternate carbon atoms, thus providing a convenient method for synthesising lipids in a form useful for nuclear magnetic resonance (NMR) studies of lipids in situ in membranes. Marine algae used in fish farming may have higher levels of very highly unsaturated fatty acids; proposals for producing these compounds labelled with carbon-13 are, therefore, presented, based on using centrally labelled glycerol. The scope for producing other substances labelled in a form suitable for NMR studies, such as carotenoids, is discussed.
authors.affiliation: Department of Nutrition and Food Science, College of Agriculture, University of Maryland at College Park, 3304 Marie Mount Hall, College Park, MD 20742-7521, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Boyle-Roden
authors.forename: J B
authors.initials: JB
authors.lastname: German
authors.forename: B J B
authors.initials: BJ
authors.lastname: Wood
mesh_terms: D002247:Carbon Isotopes / Q000737:chemistry* / Q000378:metabolism*; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry* / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy
chemical_list: D002247:Carbon Isotopes; D005231:Fatty Acids, Unsaturated; D008055:Lipids