Transduction of hematopoietic stem cells with a retroviral vector expressing the neomycin phosphotransferase gene.
Transduction of stem cells with a marking gene holds promise to determine if tissue repair or regeneration is derived from the adult hematopoietic stem cell and if relapse of an autoimmune disease should occur whether relapse arises from the stem cell compartment or from lymphocytes surviving the conditioning regimen. New safety concerns about gene-modified stem cell would entail new safety testing such as documentation of the insertional site prior to release.
Link
C J
CJ
Stoddard Cancer Research Institute, Iowa Methodist Medical Center, 1415 Woodland Ave., Des Moines, IA 50309, USA.
Seregina
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T
Edleman
M
M
Young
W-B
WB
Burt
R K
RK
Kisselev
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D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D019868:Kanamycin Kinase / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods*
D015703:Antigens, CD; D018952:Antigens, CD34; D019868:Kanamycin Kinase