The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.
Kalinowski
Jörn
J
Institut für Genomforschung, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany. joern.kalinowski@genetik.uni-bielefeld.de
Bathe
Brigitte
B
Bartels
Daniela
D
Bischoff
Nicole
N
Bott
Michael
M
Burkovski
Andreas
A
Dusch
Nicole
N
Eggeling
Lothar
L
Eikmanns
Bernhard J
BJ
Gaigalat
Lars
L
Goesmann
Alexander
A
Hartmann
Michael
M
Huthmacher
Klaus
K
Krämer
Reinhard
R
Linke
Burkhard
B
McHardy
Alice C
AC
Meyer
Folker
F
Möckel
Bettina
B
Pfefferle
Walter
W
Pühler
Alfred
A
Rey
Daniel A
DA
Rückert
Christian
C
Rupp
Oliver
O
Sahm
Hermann
H
Wendisch
Volker F
VF
Wiegräbe
Iris
I
Tauch
Andreas
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000096:biosynthesis* / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003352:Corynebacterium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014815:Vitamins / Q000096:biosynthesis* / Q000235:genetics
D000596:Amino Acids; D009097:Multienzyme Complexes; D020543:Proteome; D011994:Recombinant Proteins; D014815:Vitamins; D001224:Aspartic Acid