title: Differential interleukin-8 and nitric oxide production in epithelial cells induced by mucosally invasive and noninvasive Trypanosoma cruzi trypomastigotes.
abstract: Trypanosoma cruzi metacyclic trypomastigotes (MT), but not blood form trypomastigotes (BFT), are highly mucosally infective. We investigated the abilities of MT and BFT to induce inflammation and/or intracellular killing activity within mucosal epithelia. BFT, but not MT, induced marked increases in interleukin-8, GRO-alpha, MCP-1, and nitric oxide production in HeLa and AGS cells, despite similar infectivities. MT may avoid induction of inflammation as an important biological mechanism facilitating mucosal invasion.
authors.affiliation: Department of Internal Medicine, Saint Louis University Health Sciences Center, St. Louis, Missouri, USA.
authors.forename: C S
authors.initials: CS
authors.lastname: Eickhoff
authors.forename: L
authors.initials: L
authors.lastname: Eckmann
authors.forename: D F
authors.initials: DF
authors.lastname: Hoft
mesh_terms: D000818:Animals; D002460:Cell Line; D014355:Chagas Disease / Q000209:etiology / Q000276:immunology; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054360:Chemokine CXCL1; D018925:Chemokines / Q000096:biosynthesis; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D009092:Mucous Membrane / Q000276:immunology / Q000469:parasitology; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology
chemical_list: C475829:CXCL1 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D012333:RNA, Messenger; D009569:Nitric Oxide