title: The use of poly(ethylene glycol)-block-poly(lactic acid) derived copolymers for the rapid creation of biomimetic surfaces.
abstract: For many tissue engineering applications biomimetic or bioactive polymers would allow for a more precise control of cell behavior in growing tissues than has so far been possible. For this application recently developed amine reactive diblock copolymers (N-succinimidyl tartrate monoamine poly(ethylene glycol)-block-poly(D,L-lactic acid) [ST-NH-PEGxPLAy]) were investigated concerning their reactivity in binding model substances. Their ability to covalently immobilize proteins on their surfaces was examined using polymer films with amine reactive surfaces. Furthermore, thiol reactive polymers were obtained by attaching N-succinimidyl 3-maleinimido propionate, a thiol reactive linker to monoamine poly(ethylene glycol)-block-poly(D,L-lactic acid) [H2N-PEGxPLAy]. This allowed the immobilization of proteins carrying free thiol groups. The amine and thiol reactive polymers were characterized by 1H-NMR spectroscopy and gel permeation chromatography (GPC). Investigation of glass transitions temperatures using modulated differential scanning calorimetry proved suitability for the fabrication of polymeric scaffolds for tissue engineering applications. The functionality of the polymers was demonstrated by investigating their ability to bind model amines, like the fluorescent dye EDANS. Moreover, insulin and somatostatin were covalently attached to the active linker groups via amine and thiol groups. The polymers will permit covalently attaching different bioactive molecules, such as growth and differentiation factors, with fast and gentle procedures securing their biological activity.
authors.affiliation: Department of Pharmaceutical Technology, University of Regensburg, Regensburg 93040, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Tessmar
authors.forename: Antonios
authors.initials: A
authors.lastname: Mikos
authors.forename: Achim
authors.initials: A
authors.lastname: Göpferich
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D007328:Insulin / Q000737:chemistry; D007773:Lactates / Q000737:chemistry*; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols / Q000737:chemistry*; D011487:Protein Conformation; D013004:Somatostatin / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D007328:Insulin; D007773:Lactates; D013438:Sulfhydryl Compounds; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D013004:Somatostatin