New red blood cell lysing fixative for use in fine needle aspiration and fluid cytology.
OBJECTIVE
To describe a new red blood cell (RBC) lysing fixative, Devine's lysing solution (DLS), that increases the diagnostic utility of fine needle aspiration (FNA) and fluid cytology.


METHODS
Twenty bloody FNA cases were fixed with either DLS or 95% ethanol, and the ability to render a diagnosis on these materials was analyzed. DLS was compared to the red cell lysing fixative CytoRich Red (CRR) (TriPath Care Technologies, Burlington, North Carolina, U.S.A.) in its ability to lyse RBCs by mixing human RBCs with the U266 multiple myeloma cell line. DLS was compared to CRR in its ability to suitably preserve materials for Papanicolaou and immunocytochemical analysis.


RESULTS
Comparison of DLS with 95% ethanol fixation in 20 bloody FNA cases prepared in duplicate showed that DLS reduced from 17 to 3 the number of cases that had RBCs obscuring > or = 25% of the diagnostic material. In 3 cases, DLS enabled the rendering of a definitive diagnosis of malignancy, which could not be made on the ethanol-fixed material. DLS was compared to CRR, and both fixatives were similarly effective at lysing RBCs, preserving the cellular morphology of diagnostic cells in FNA and fluid cytology, and preserving cells for use in immunocytochemistry.


CONCLUSIONS
DLS increases the visualization of diagnostic cellular material when compared to ethanol fixation. DLS is comparable to CRR in RBC lysing ability, diagnostic cell preservation and preservation of materials for immunocytochemistry.
Davis-Devine
Sharon
S
Carle Clinic Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Day
Sarah J
SJ
Freund
Gregory G
GG
D016477:Artifacts; D001707:Biopsy, Needle / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology; D005404:Fixatives / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D015203:Reproducibility of Results; D016707:Tissue Fixation / Q000379:methods*; D014407:Tumor Cells, Cultured
D005404:Fixatives; D000431:Ethanol