title: Changes in temperature modulate heme oxygenase-1 induction by curcumin in renal epithelial cells.
abstract: The stress protein heme oxygenase-1 (HO-1) plays an essential role in the prevention of transplant-associated organ injury and rejection. Prior to transplantation, organs are normally subjected to variable periods of cold storage in appropriate preservation solutions. Here, we examined whether curcumin, a phenolic plant extract which strongly induces HO-1 in many cell types, could up-regulate HO-1 protein in cultured renal epithelial cells at temperatures lower than the physiological 37 degrees C. We found that stimulation of HO-1 following incubation of cells with curcumin for 6h was dramatically reduced by decreasing the temperature from 37 to 10 degrees C. Interestingly, renal cells displayed high HO-1 expression and heme oxygenase activity when exposed to a programmed change in temperature that consisted of 3h at 37 degrees C followed by 1.5h at 20 degrees C and 1.5h at 10 degrees C. Increased HO-1 levels were observed also after incubation of cells with curcumin during the programmed change in temperature under hypoxia, another feature typical of cold storage procedures. Upon challenge with an oxidant-generating system, cells pretreated with curcumin at 37 degrees C or during the programmed change in temperature exhibited increased resistance to oxidative stress-mediated injury. These findings highlight the feasibility of modulating HO-1 expression during hypothermic storage to confer tissues a better protection to counteract the damage characteristic of organ transplantation.
authors.affiliation: Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex HA1 3UJ, UK.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Balogun
authors.forename: Roberta
authors.initials: R
authors.lastname: Foresti
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Green
authors.forename: Roberto
authors.initials: R
authors.lastname: Motterlini
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival; D003474:Curcumin / Q000378:metabolism*; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000737:chemistry* / Q000378:metabolism*; D051547:Heme Oxygenase-1; D000860:Hypoxia; D007668:Kidney / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D013552:Swine; D013696:Temperature; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D016877:Oxidants; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D003474:Curcumin