Chemical analysis and sediment toxicity bioassays to assess the contamination of the River Lambro (Northern Italy).
This study consisted of a 10-day whole sediment toxicity test with the chironomid Chironomus riparius and a 28-day sediment toxicity test with the oligochaete Tubifex tubifex at seven sites to assess the quality of the River Lambro (Italy), one of the most contaminated rivers of the Po Basin. Endpoints measured were survival and growth for chironomids and cocoon deposition and development of young worms for tubificid oligochaetes. Responses were evaluated in relation to the occurence of organic micropollutants (PCBs, DDT, HCB, and HCH) representative of the industrial and agricultural contamination of the area. Though survival of the organisms remained unaffected, sublethal effects were observed at all sites. The sediment sampled at the farthest upstream site differed from the control only in the number of cocoons deposited by the worms. Both test species in the next three sediments, where concentrations of PCB and DDT were in the range 21.9-39.5 ng g(-1) DW and 0.6-1.3 ng g(-1) DW, respectively, experienced greater toxicity in terms of growth and reproduction. Contamination was particularly high in the site closest to Milan, where the river receives untreated urban and industrial discharges. Levels of total PCBs and total DDT here were up to two orders of magnitude higher than those found at the other sampling locations, and chironomid growth and the reproductive endpoints of tubificids were significantly lower than in the control and the other sites. The test results for the next two stations showed improvement relative to that of Milan, although contamination was still evident. Sublethal effects were in agreement with the measured concentrations of the principal persistent organic pollutants and should be included as part of environmental monitoring efforts as a basis for assessing the recovery of the river.
Bettinetti
R
R
Università degli Studi di Milano, Biology Department, Ecology Section, Via Celoria 26, Milan, Italy. roberta.bettinetti@tiscali.it
Giarei
C
C
Provini
A
A
D000818:Animals; D002683:Chironomidae; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D019015:Geologic Sediments / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis / Q000633:toxicity*; D007558:Italy; D009835:Oligochaeta; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D004785:Environmental Pollutants; D007306:Insecticides; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls