Theozyme for antibody aldolases. Characterization of the transition-state analogue.
A theozyme for antibody aldolases has been studied at the MP2/6-31G** computational level. Formation of two cooperative hydrogen-bonds between the acidic hydrogen atoms of the enamine and of a methanol molecule with the oxygen atom of the aldol acceptor markedly favors the C-C bond-formation associated with the aldol reaction. A comparative analysis of the geometry, the charge distribution and the shape of the molecular electrostatic potential of the transition structure (TS) with the covalent adduct, resulting from the reaction of methylamine and the beta-diketone used as a hapten allows us to characterize the transition-state analogue (TSA) generated at immunization. This finding allows us to propose a hapten based on a chiral beta-ketosulfoxide that could give the formation of a TSA that addresses the tetrahedral geometry of the TS.
Arnó
Manuel
M
Instituto de Ciencia Molecular, Departamento de Química Orgánica, Universidad de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain.
Domingo
Luis R
LR
D000446:Aldehyde-Lyases / Q000737:chemistry*; D000588:Amines / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D002384:Catalysis; D000432:Methanol / Q000737:chemistry; D008958:Models, Molecular
D000588:Amines; D000906:Antibodies; D000446:Aldehyde-Lyases; D000432:Methanol