Molecular evidence for transferrin receptor 2 expression in all FAB subtypes of acute myeloid leukemia.
We examined transferrin receptor (TfR) 1 and TfR2 mRNA expression in 50 acute myeloid leukemia (AML) patients by RT-PCR, with primers specific for exons 15-17 (TfR1), 3-5 (TfR2-alpha) and 4-5 (TfR2-beta) of the corresponding gene. There were 4/50 TfR1-negative (-), 3/50 TfR2-alpha mRNA (-) and 13/50 TfR2-beta mRNA (-) cases; only three cases were TfR1/2 mRNA (-). No significant correlations were identified between TfR2-beta mRNA negativity and specific FAB subtypes, karyotype or attainment of complete remission. These findings suggest that: (i) TfR2 expression is not restricted to erythroid cells, and (ii) iron import proteins might complement each other in AML cells.
Kollia
Panagoula
P
Department of Biology, University of Thessaly, Larissa, Greece. kollia@med.uth.gr
Samara
Maria
M
Stamatopoulos
Kostas
K
Belessi
Chrysoula
C
Stavroyianni
Niki
N
Tsompanakou
Aliki
A
Athanasiadou
Anastasia
A
Vamvakopoulos
Nikos
N
Laoutaris
Nikos
N
Anagnostopoulos
Achilles
A
Fassas
Athanasios
A
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics*; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D007140:Immunoglobulin Fab Fragments; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011990:Receptors, Transferrin; C503640:TFR2 protein, human; D007501:Iron