Determination of bisphenol A in milk and dairy products by high-performance liquid chromatography with fluorescence detection.
This study was conducted to develop a selective and sensitive method for the determination of bisphenol A (BPA) levels in milk and dairy products. A method based on solvent extraction with acetonitrile and solid-phase extraction (SPE) was developed for the analysis of BPA in milk, yogurt, cream, butter, pudding, condensed milk, and flavored milk, and a method using two SPE cartridges (OASIS HLB and Florisil cartridge) for skim milk was also developed. The developed methods showed good recovery levels (77 to 102%) together with low detection limits (1 microg/liter for milk, yogurt, pudding, condensed milk, flavored milk, and skim milk and 3 microg/liter for cream and butter). These methods are simple, sensitive, and suitable for the analysis of BPA in milk and dairy products. When 40 milk and dairy products were analyzed by the proposed methods, BPA was not identified in noncanned products, but its levels ranged from 21 to 43 microg/kg in canned products, levels that were 60- to 140-fold lower than the migration limits in the European Union and Japan.
Kang
Jeong-Hun
JH
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Kibanadai-Nishi, Gakuen, Miyazaki-shi 889-2192, Japan.
Kondo
Fusao
F
D000818:Animals; D001559:Benzhydryl Compounds; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds