title: The role and expression of CD27 and CD70 lymphocytes in the human tonsil.
abstract: In this study, we investigated the expression and function of CD27 and CD70 in the human tonsil. We found that IgG production was significantly reduced after the administration of anti-CD27 and anti-CD70 antibodies in the T cell dependent B cell activation system, suggesting the involvement of CD27/CD70 in the production of IgG. In addition, the amounts of peripheral and tonsililar CD4+ CD27+ cells were almost identical, whereas the expression of CD4+ CD27- cells was higher (4.9%) in tonsil than in peripheral blood (2.4%). Furthermore, anti-CD27 antibody and anti-CD4 antibody-positive cells were found mainly in the interfollicular region, and a small number of this cell population was observed in both germinal center (GC) and mantle. Double-staining showed that the germinal center was almost completely composed of CD4+ CD27+ cells. In contrast to CD27 cells, only a small number of CD70+ cells were present in the follicles. Moreover, anti-CD19 antibody, a B cell marker, was stained in the GC and mantle zone, and CD19+ CD27+ cells were observed in the GC close to the mantle. The above findings favor the involvement and role of CD27/CD70 in the T-B cell interaction by inducing IgG production and increasing memory cell (CD4+ CD27-) number in the tonsil.
authors.affiliation: Department of Otorhinolaryngology, Juntendo University School of Medicine, Tokyo, Japan. hiddy@aurora.dti.ne.jp
authors.forename: H
authors.initials: H
authors.lastname: Yokoi
authors.forename: M
authors.initials: M
authors.lastname: Seki
authors.forename: S
authors.initials: S
authors.lastname: Okazoe
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D053282:CD27 Ligand; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D014066:Palatine Tonsil / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; D007074:Immunoglobulin G; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7