[An experimental study of inhibiting effect of decoy oligodeoxynucleotides on the gene expression of collagen in NIH3T3 cell].
OBJECTIVE
To investigate the effect of activator protein-1 (AP-1) decoy-oligodeoxynucleotides (Decoy-ODNs) on the expression of fibroblast alpha2 type I collagen, so as to explore the gene therapy of pathologic scar.


METHODS
Decoy-ODNs targeting AP-1 were designed and synthesized. NIH3T3 cells were transfected by cationic liposomes. The distribution of Decoy-ODNs in the cells was investigated. The inhibiting effects of Decoy-ODNs on AP-1 were determined by electrophoretic mobility shift assay (EMSA). And the effects of Decoy-ODNs on the collagen synthesis in the cells were analyzed by RT-PCR.


RESULTS
AP-1 Decoy-ODNs could competitively inhibit the AP-1 in vitro activity. Cationic liposomes could play roles by effectively transfecting Decoy-ODNs into the plasma and nucleus. The mRNA expression of fibroblast alpha2 type I collagen decreased evidently after 24 hours of Decoy-ODNs action.


CONCLUSIONS
Decoy-ODNs could inhibit the mRNA expression of fibroblast alpha2 type I collagen by antagonizing AP-1.
Hao
Tian-zhi
TZ
Department of neurosurgery, Research Institute of surgery, Daping Hospital, Third military Medical University, Chongqing 400042, PR China.
Liang
Huan-ping
HP
Lv
Feng-lin
FL
Xu
Xiang
X
Guo
Hua
H
Wang
Fu-long
FL
Yang
Wen-jun
WJ
Luo
Yan
Y
Li
Lei
L
D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014162:Transfection
D024042:Collagen Type I; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D018808:Transcription Factor AP-1