title: [Expression of alpha-SMA mRNA in the lung tissue of rat with asthma].
abstract: OBJECTIVE
  To investigate the expression of smooth muscle actin(SMA)-alpha mRNA in the lung of rat with asthma.
  
  
  METHODS
  A rat model of asthma was established by exposing the animal to aerosolized ovalbumin (OA). After being sensitized, the rat was subjected to laboratory tests. alpha-SMA mRNA of the lung tissue was measured by RT-PCR. The thickness of the airway smooth muscle(ASM) was measured by an image analysis system at the same time.
  
  
  RESULTS
  The thickness of ASM increased 2 weeks after OA challenge. The expression of alpha-SMA mRNA increased 1 week after OA challenge and increased more significantly after 2 weeks.
  
  
  CONCLUSIONS
  In the rat model of asthma, hypertrophy and hyperplasia of the airway smooth muscle were noted, and the phenotype was changed.
authors.affiliation: Department of Respiratory Disease, 452 Hospital of People's Liberation Army, Chengdu 610041, China.
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Wu
authors.forename: Fengming
authors.initials: F
authors.lastname: Luo
authors.forename: Zengli
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.forename: Chuntao
authors.initials: C
authors.lastname: Liu
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism* / Q000473:pathology; D010047:Ovalbumin; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats
chemical_list: D000199:Actins; D012333:RNA, Messenger; D010047:Ovalbumin