title: Urinary excretion of 8-hydroxy-2-deoxyguanosine and 1-hydroxypyrene in coke-oven workers.
abstract: Coke-oven workers (COWs) are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons (PAH). Urinary 8-hydroxy-2-deoxyguanosine (8-OH-dG) and 1-hydroxypyrene (1-OHP) are biological markers of oxidative DNA damage and PAH metabolism, respectively. We investigated the relationship between urinary 8-OH-dG and 1-OHP in 217 Taiwanese COWs, 55 topside-oven, and 162 sideoven workers. For topside-oven workers, mean 8-OH-dG and 1-OHP concentrations (ng/ml +/- SD) were 13.8 +/- 12.0 and 93.5 +/- 104.4, respectively. These levels were significantly higher than those for sideoven workers: 10.2 +/- 7.9 ng/ml (P = 0.04) and 19.8 +/- 28.6 ng/ml (P < 0.001), respectively. Individual urinary 8-OH-dG concentrations were directly correlated with urinary 1-OHP concentrations: the higher the 1-OHP level, the higher the 8-OH-dG level (Spearman correlation coefficients: r = 0.43, P < 0.0001, n = 217). Multiple regression analysis indicated that a 10-fold increase in 1-OHP was associated with a 1.91-fold increase in 8-OH-dG. Compared to no vitamin intake, intake of at least one multiple vitamin pill per week reduced 8-OH-dG excretion (P = 0.02). Our findings suggest that urinary 1-OHP and 8-OH-dG reflect occupational PAH exposure and oxidative DNA damage in COWs. In addition, multiple vitamins may reduce oxidative stress caused by PAH exposure.
authors.affiliation: Graduate Institute of Occupational Safety and Health and Department of Occupational Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. mingsangwu@yahoo.com
authors.forename: Ming-Tsang
authors.initials: MT
authors.lastname: Wu
authors.forename: Chih-Hong
authors.initials: CH
authors.lastname: Pan
authors.forename: Yeou-Lih
authors.initials: YL
authors.lastname: Huang
authors.forename: Perng-Jy
authors.initials: PJ
authors.lastname: Tsai
authors.forename: Chiou-Jong
authors.initials: CJ
authors.lastname: Chen
authors.forename: Trong-Neng
authors.initials: TN
authors.lastname: Wu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers; D003077:Coke / Q000009:adverse effects*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D004522:Educational Status; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000378:metabolism; D016273:Occupational Exposure; D010084:Oxidation-Reduction; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D011721:Pyrenes / Q000378:metabolism*; D012044:Regression Analysis; D012907:Smoking
chemical_list: D015415:Biomarkers; D003077:Coke; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C033146:1-hydroxypyrene