title: Binding studies on aluminum(III)-albumin interaction.
abstract: The present paper reports a quantitative investigation on the binding of aluminum to human serum albumin. Equilibrium dialysis and a general thermodynamic approach have been used to determine the binding parameters. Two aluminum binding sites have been identified on the albumin molecule, involving sites with single and double occupancy modes. The binding properties of these two distinct sites appear to undergo reciprocal influences, suggesting a possible interaction between the corresponding protein moieties. Both coordination modes proceed from weak interactions, as shown by the binding energies calculated.
authors.affiliation: CNR-Institute for Biomedical Technologies, Metalloproteins Unit, Viale G. Colombo, 3, 35121, Padua, Italy. zatta@mail.bio.unipd.it
authors.forename: Paolo
authors.initials: P
authors.lastname: Zatta
authors.forename: Lisa
authors.initials: L
authors.lastname: Dalla Via
authors.forename: Vito
authors.initials: V
authors.lastname: Di Noto
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D001665:Binding Sites; D006801:Humans; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D012709:Serum Albumin; D000535:Aluminum