title: Concomitant induction of apoptosis and expression of monocyte chemoattractant protein-1 in cultured rat luteal cells by nuclear factor-kappaB and oxidative stress.
abstract: It has previously been shown that expression of monocyte chemoattractant protein (mcp)-1 and apoptosis of luteal cells occur concomitantly during the estrous cycle in the rat corpus luteum; however, luteal cells containing mcp-1 mRNA did not seem to be apoptotic. In the present study, the relationship between the induction of apoptosis and mcp-1 expression in cultures of dispersed rat luteal cells was examined. Both apoptosis and mcp-1 expression were spontaneously induced in cultured luteal cells in a manner inhibitable by antioxidative reagents or an inhibitor of nuclear translocation of nuclear factor-kB. However, the cells containing mcp-1 mRNA were distinct from those undergoing apoptosis, and the inhibition of apoptosis by the pan-caspase inhibitor z-VAD-fmk did not influence the induction of mcp-1 expression. These results collectively indicate that oxidative stress simultaneously, but independently, induces apoptosis and mcp-1 expression in luteal cells through the activation of nuclear factor-kB. This phenomenon might help to explain how monocytes/macrophages accumulate in regressive corpora lutea where their target apoptotic cells exist.
authors.affiliation: Graduate School of Natural Science and Technology, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920-0934, Japan.
authors.forename: Kaz
authors.initials: K
authors.lastname: Nagaosa
authors.forename: Akiko
authors.initials: A
authors.lastname: Shiratsuchi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D018932:Chemokine CCL2 / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000502:physiology*; D016328:NF-kappa B / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats
chemical_list: D018932:Chemokine CCL2; D016328:NF-kappa B