[Influence of huangqi on TGF-beta 1 inducing extracellular matrix secretion of human peritoneal mesothelial cell].
OBJECTIVE
To study the effect of huangqi on peritoneal sclerosis and its possible mechanism.


METHODS
Isolated human peritoneal mesothelial cells (HPMC) was cultured. Cultured HPMC were treated with F12 without serum (control group), F12 with TGF-beta 1 (TGF-beta 1 group), F12 with TGF-beta 1 and huangqi 100 micrograms/ml (huangqi 1 group), F12 with TGF-beta 1 and huangqi 1 mg/ml (huangqi 2 group). Fibronectin(FN) and plasminogen activator inhibitor-1 (PAI-1) were detected by ELISA method. The expression of FN mRNA, PAI-1 mRNA and bFGF mRNA was detected by RT-PCR method.


RESULTS
1. HPMC expressed FN, PAI-1, and bFGF. 2. Fn and PAI-1 significantly increased compared with control with 5 ng/ml TGF-beta 1 stimulated at 24 hours and 48 hours(P < 0.05); 3. The different concentration of huangqi decreased FN and PAI-1 expression compared with TGF-beta 1 group, especially 1 mg/ml huangqi at 24 hours(P < 0.05). 4. FN, PAI-1 and bFGF mRNA expression were higher in 12 hours after stimulation with TGF-beta 1 compared with control. FN, PAI-1 and bFGF mRNA expression were lower in 12 hours after stimulation with different concentration of huangqi (100 micrograms/ml and 1 mg/ml) than those in the TGF-beta 1 group.


CONCLUSIONS
TGF-beta 1 promotes extracellular matrix synthesis and secretion of HPMC. Huangqi partly counteracts the synthesis of human peritoneal mesothelial cell's extracellular matrix by the stimulation of TGF-beta 1.
Liu
Ying-hong
YH
Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Liu
Fu-you
FY
Duan
Shao-bing
SB
D027883:Astragalus Plant; D027885:Astragalus propinquus; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004848:Epithelium / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D004365:Drugs, Chinese Herbal; D005353:Fibronectins; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C027492:Huang Qi