Improving sensitivity by large-volume sample stacking using the electroosmotic flow pump to analyze some nonsteroidal anti-inflammatory drugs by capillary electrophoresis in water samples.
Large-volume sample stacking using the electroosmotic flow (EOF) pump (LVSEP) has been used to analyze some nonsteroidal anti-inflammatory drugs (NSAIDs) in water samples. With methanol as the run buffer solvent to suppress the EOF, sensitivity was enhanced by 80-100-fold. The sample for the analysis of real water sample was pretreated by solid-phase extraction (SPE). When the method was based on off-line SPE-LVSEP-CE, sensitivity improved by as much as 1000 times.
Macià
Alba
A
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Plaça Imperial Tàrraco 1, E-43005 Tarragona, Spain.
Borrull
Francesc
F
Aguilar
Carme
C
Calull
Marta
M
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000302:isolation & purification*; D002021:Buffers; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D009995:Osmosis; D015203:Reproducibility of Results; D012997:Solvents; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002021:Buffers; D012997:Solvents; D014874:Water Pollutants, Chemical