RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong.
A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3D(pol) gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3D(pol) gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3D(pol) sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3D(pol) gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3D(pol) gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D(pol) amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.
Chen
Xi
X
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Feng
Qian
Q
Wu
Zhiyin
Z
Liu
Yingying
Y
Huang
Kun
K
Shi
Rui
R
Chen
Sidi
S
Lu
Wen
W
Ding
Mingxiao
M
Collins
Richard A
RA
Fung
Yin-Wan Wendy
YW
Lau
Lok-Ting
LT
Yu
Albert Cheung-Hoi
AC
Chen
Jianguo
J
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology* / Q000235:genetics*; D014644:Genetic Variation; D006723:Hong Kong; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases