Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science and molecular diagnostics.
Hanselle
Thomas
T
QIAGEN GmbH, Max-Volmer Strasse 4, 40724 Hilden, Germany. Thomas.Hanselle@qiagen.de
Otte
Michael
M
Schnibbe
Thomas
T
Smythe
Emma
E
Krieg-Schneider
Frank
F
D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D006801:Humans; D009061:Mouth Mucosa / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012822:Silicon Dioxide; D020080:Tandem Repeat Sequences
D012822:Silicon Dioxide; D004247:DNA