[Selection of targets locating on the surface of epithelial ovarian cancer cells by using phage peptide library].
OBJECTIVE
The aim of this study was to select specific targets locating on the surface of epithelial ovarian cancer cells.


METHODS
Peptide phage display library was used to isolate specific ligand to ovarian cancer cell receptors. The diluted library was incubated with the normal ovarian cells that primarily cultured before hand, and then the supernatant of nonbonding phage was added to the first epithelial ovarian cancer cell line A2780. Phage that binds to the cell surface are eluted and then amplified to be used in the next round. After the third round of panning, the elute was used as input phage for the next cell line biopanning. Four epithelial ovarian cancer cell lines were used one by one in this way. Finally the positive clones were identified by ELISA assay. Sequencing analysis was carried out for further identification.


RESULTS
10 positive clones were chosen and one was regarded as target clone. A candidate sequence (YYGLAEVDAGGS) was identified by amino acid sequence assay.


CONCLUSIONS
The phage peptide library provides an efficient selection system for searching special targets locating on the cell surface.
Wang
Shi-xuan
SX
Department of Obstetrics and Gynecology, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhou
Hong-yuan
HY
Ma
Xiang-yi
XY
Chen
Gang
G
Lu
Yun-ping
YP
He
Fu-xian
FX
Ma
Ding
D
D005260:Female; D006801:Humans; D008024:Ligands; D010051:Ovarian Neoplasms / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D011956:Receptors, Cell Surface