Development of a triplex PCR assay for the specific detection of Campylobacter jejuni, Salmonella spp., and Escherichia coli O157:H7.
A triplex PCR assay was developed and evaluated for efficacy in detecting Campylobacter jejuni, Salmonella spp., and Escherichia coli O157:H7 in a variety of raw and ready-to-eat food products. Following a short enrichment period, artificially contaminated food samples were subjected to a triplex PCR assay, which incorporated published primers for each food pathogen, a protocol for sample collection, and a PCR procedure designed specifically for the assay. The selected primers amplified fragment sizes of 159 bp, 252 bp, and 360 bp for C. jejuni, E. coli O157:H7, and Salmonella spp., respectively. This assay provides specific and reliable results and allows for the cost-effective detection of all three bacterial pathogens in one reaction tube.
Gilbert
Carl
C
Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, POSC O-310, Fayetteville, AR 72701, USA.
Winters
Debra
D
O'Leary
Awilda
A
Slavik
Michael
M
D001431:Bacteriological Techniques; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012680:Sensitivity and Specificity
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial