title: Beta-secretase (BACE) and GSK-3 mRNA levels in Alzheimer's disease.
abstract: Beta-secretase (BACE) and glycogen synthase kinase (GSK 3) are two enzymes thought to play a role in Alzheimer's disease. We extracted mRNA from 90 Alzheimer and 81 control brains. Levels of mRNA were quantified for BACE and GSK 3 with TaqMan real-time RT-PCR. We found no change in the Alzheimer's disease brains relative to controls for either the BACE or the GSK 3alpha mRNA levels.
authors.affiliation: Quantuum, Departamento de Neurología, Rua das Brañas 7-bajo-D, Mera, La Coruña, Galicia 15177, Spain. pablo@quantuum.com
authors.forename: Paul
authors.initials: P
authors.lastname: Preece
authors.forename: David J
authors.initials: DJ
authors.lastname: Virley
authors.forename: Moheb
authors.initials: M
authors.lastname: Costandi
authors.forename: Robert
authors.initials: R
authors.lastname: Coombes
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Moss
authors.forename: Anne W
authors.initials: AW
authors.lastname: Mudge
authors.forename: Elena
authors.initials: E
authors.lastname: Jazin
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Cairns
mesh_terms: D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D053829:Amyloid Precursor Protein Secretases; D016564:Amyloid beta-Protein Precursor / Q000032:analysis / Q000235:genetics; D016282:Aspartic Acid Endopeptidases; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000235:genetics; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012727:Sex Characteristics
chemical_list: D016564:Amyloid beta-Protein Precursor; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D038362:Glycogen Synthase Kinase 3; D053829:Amyloid Precursor Protein Secretases; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human; D010751:Phosphopyruvate Hydratase