title: Improved preservation of coagulation factors after pre-storage leukocyte depletion of whole blood.
abstract: Plasma and red blood cell quality are affected both by citrate concentration and the levels of extracellular leukocyte and platelet derived substances, accumulated during storage of blood. The effect of leukocyte filtration on the storage stability of whole blood was therefore studied in blood collected in standard CPD and 0.5CPD (CPD with half strength citrate concentration). A total of 52 units, 12 of them with reduced citrate concentration, were leukocyte-filtered with Pall( whole blood filter (WBF1 or 3). No differences in leukocyte or platelet reduction were observed with the two citrate concentrations. However, with 0.5CPD a significantly longer filtration time and increased complement activation was observed. The effect of pre-storage leukocyte filtration on the plasma quality of whole blood was therefore only studied with standard CPDA1 anticoagulant solution (normal strength citrate concentration). Leukocyte filtration did not affect the von Willebrand factor concentration, while a small reduction (7%, p=0.04) in factor VIII (FVIII) concentration was observed. During storage, however, FVIII decreased more slowly in the filtered than in the unfiltered product, and, from day two, the FVIII content was significantly higher in the filtered product (46% versus 30% at 28 days, p<0.001). Factor V (FV) demonstrated a 16% reduction (p<0.001) upon filtration, followed by an additional 8% in the next 24 h and only a 4% reduction the next 27 days, while unfiltered products demonstrated a continuous reduction to 26% at 28 days. While the beta-thromboglobulin (beta-TG) concentration significantly increased (from 836 to 2483 IU/ml, p<0.001) during leukocyte filtration, no further increase was observed during storage. In contrast, unfiltered products demonstrated an increase to 5762 IU/ml (p<0.001) at 14 days, followed by a slight, not significant, reduction. This indicates platelet activation during filtration and explains a parallel reduction in FV. Filtration induced no increase in prothrombin fragment 1+2, while a slight increase was observed in some unfiltered products after 28 days of storage.Pre-storage leukocyte depletion thus improves the coagulation factor content of plasma in stored whole blood.
authors.affiliation: Institute of Immunology, Medical Department, Rikshospitalet University Hospital, NO-0027 Oslo, Norway. bjarte.solheim@rikshospitalet.no
authors.forename: B G
authors.initials: BG
authors.lastname: Solheim
authors.forename: O
authors.initials: O
authors.lastname: Flesland
authors.forename: F
authors.initials: F
authors.lastname: Brosstad
authors.forename: T E
authors.initials: TE
authors.lastname: Mollnes
authors.forename: J
authors.initials: J
authors.lastname: Seghatchian
mesh_terms: D001779:Blood Coagulation Factors / Q000032:analysis*; D001793:Blood Preservation; D005169:Factor VIII / Q000378:metabolism; D005374:Filtration / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007937:Leukapheresis / Q000379:methods*; D007958:Leukocyte Count; D010976:Platelet Count; D013997:Time Factors; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D001779:Blood Coagulation Factors; D014841:von Willebrand Factor; D005169:Factor VIII