title: Molecular cytogenetic approach to the diagnosis of splenic lymphoma: a case report of blastoid mantle cell lymphoma.
abstract: A 69-year-old woman was admitted to hospital due to abdominal pain. An enlarged spleen was detected and extirpated. Histological examination and FACS analysis could not clearly differentiate between a splenic marginal zone lymphoma and a mantle cell lymphoma. Using molecular cytogenetic techniques, a complex karyotype including a translocation between chromosome 11 and 14 consistent with a triple fusion between heavy chain immunoglobulin locus (IgH) and cyclin D1 (CCND1) was demonstrated. Overexpression of cyclin D1 was detected by immunostaining. Thus the diagnosis of a splenic blastoid mantle cell lymphoma could be established. In this report, various aspects of differential diagnosis of splenic lymphomas are discussed.
authors.affiliation: Department of Molecular Medicine, CMM L8:02 Karolinska Hospital and Institutet, SE-171 76, Stockholm, Sweden. erik.bjorck@cmm.ki.se
authors.forename: Erik
authors.initials: E
authors.lastname: Björck
authors.forename: Ola
authors.initials: O
authors.lastname: Landgren
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Schoumans
authors.forename: Birger
authors.initials: B
authors.lastname: Christensson
authors.forename: Magnus
authors.initials: M
authors.lastname: Björkholm
authors.forename: Anna Porwit
authors.initials: AP
authors.lastname: MacDonald
authors.forename: Magnus
authors.initials: M
authors.lastname: Nordenskjöld
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D019938:Cyclin D1 / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D013160:Splenic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: D019938:Cyclin D1