[Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry].
OBJECTIVE
To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells.


METHODS
The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay.


RESULTS
The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro.


CONCLUSIONS
The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time dependent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.
Yu
Jun-long
JL
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Yu
Ping
P
Li
Li-xin
LX
D002692:Chlamydia trachomatis / Q000254:growth & development / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*
D016209:Interleukin-8; D016753:Interleukin-10