title: Use of pulsed-field gel electrophoresis in infection control issues concerning Burkholderia cepacia.
abstract: There was concern that nosocomial person-to-person transmission of Burkholderia cepacia had occurred when two patients with cystic fibrosis shared a bathroom. Pulsed-field gel electrophoresis demonstrated that the two isolates were unrelated. Subsequent testing of 34 stored isolates of B. cepacia demonstrated that no particular clone predominated in our hospital.
authors.affiliation: Microbiology Department, The Alfred Hospital, Melbourne, Victoria, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Jenney
authors.forename: Lisa
authors.initials: L
authors.lastname: Liolios
authors.forename: Denis
authors.initials: D
authors.lastname: Spelman
authors.forename: Philip
authors.initials: P
authors.lastname: Russo
authors.forename: John
authors.initials: J
authors.lastname: Wilson
authors.forename: Steven
authors.initials: S
authors.lastname: Wesselingh
authors.forename: Tom
authors.initials: T
authors.lastname: Kotsimbos
mesh_terms: D019121:Burkholderia Infections / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D016956:Burkholderia cepacia / Q000235:genetics* / Q000302:isolation & purification; D003428:Cross Infection / Q000175:diagnosis* / Q000517:prevention & control / Q000635:transmission; D003550:Cystic Fibrosis / Q000150:complications; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017053:Infection Control / Q000379:methods*; D010356:Patient Isolation; D010641:Phenotype; D013183:Sputum / Q000382:microbiology; D014739:Victoria