[Chromatographic behavior of fat-soluble vitamin in reversed phase liquid chromatography with water-isopropanol mobile phase].
The separation of a mixture fat-soluble vitamin A, D, E by reversed-phase HPLC on elution with an isopropanol-water was studied. A procedure was developed for determining vitamin mixtures and was applied to the analysis of 9 multivitamin tablet. The vitamin was fully separated in 15 min using a short C18 column and UV detection at 280 nm. Different solvent were used for sample extraction. The recoveries were measured, and were higher than 95%. Experimental results proved that the three fat-soluble vitamin A, D, E all can be fully eluted by a solution of isopropanol-water in the ratio of 75%:25%.
Ha
Sha-er
SE
Xinjiang Analytical and Testing Center, Urumqi 830011, China.
Ta
Yi-er
YE
D019840:2-Propanol; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012995:Solubility; D014801:Vitamin A / Q000032:analysis*; D014807:Vitamin D / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D014801:Vitamin A; D014807:Vitamin D; D014810:Vitamin E; D019840:2-Propanol