[Study on the direct interaction between alkaline phophatase and Cu(II) ions by spectral analysis].
In this paper, the results of EPR, VIS and enzyme activity show that alkaline phophatase from calf intrest directly interacted with additional Cu(II). The added Cu(II) enter into A site of active sites of AKP which decrease enzyme activity. With increase of pH value, the decrease in the intensity of the Cu(II) EPR signal and the change of place signal in VIS were observed. These results suggest that Cu(II) migrate in the active center of the enzyme.
Wang
J
J
Department of Bioengineering, Dalian University of Technology, 116012 Dalian.
Zheng
X
X
An
L
L
Gai
H
H
Hu
J
J
D000469:Alkaline Phosphatase / Q000737:chemistry*; D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D004347:Drug Interactions; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration
D003300:Copper; D000469:Alkaline Phosphatase