title: Thermodynamic study of hybridization properties of heterochiral nucleic acids.
abstract: Heterochiral DNA and RNA heptamers, which contained an unnatural L-nucleotide, were synthesized, and thermodynamic analyses of their hybridization properties with complementary DNA and RNA strands were systematically conducted by UV melting experiments. The results clearly demonstrated that the incorporation of an L-ribonucleotide into the RNA strand leads to more significant destabilization of the duplexes than that of an L-deoxyribonucleotide into the DNA strand, regardless of whether the complementary strand is DNA or RNA. The destabilization of the duplexes by the substitution of D-thymidine with L-thymidine in the DNA strand is entropically driven, whereas that by the substitution of D-uridine with L-uridine in the RNA strand is enthalpically driven. The thermodynamic characteristic that the stability of homochiral duplex is far superior to that of heterochiral duplex is much more remarkable in RNA than in DNA. Thus, RNA might have been a self-replicating system superior to DNA to exclude the chiral antipode.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, Japan. urata@gly.oups.ac.jp
authors.forename: Hidehito
authors.initials: H
authors.lastname: Urata
authors.forename: Hana
authors.initials: H
authors.lastname: Shimizu
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Hiroaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kohda
authors.forename: Masao
authors.initials: M
authors.lastname: Akagi
mesh_terms: D004247:DNA / Q000737:chemistry*; D019277:Entropy; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D013936:Thymidine / Q000737:chemistry; D014466:Ultraviolet Rays; D014529:Uridine / Q000737:chemistry
chemical_list: D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine