Nylon/DNA: Single-stranded DNA with a covalently stitched nylon lining.
The synthesis of DNA/nylon ladder oligomers is described. Three stages of the development are addressed: the synthesis of 2'-beta-substituted phosphoramidites, the deprotection/purification protocols of ODNs modified with both amino and carboxyl groups, and amide bond-forming reactions on the ODNs. The established technology and the novel DNA-based ladder oligomer structure opens a pathway to the synthesis of topological molecular objects and networks templated by DNA through versatile DNA nanotechnology. The DNA-based ladder oligomers may find application in the antisense area.
Zhu
Lei
L
Department of Chemistry, New York University, New York, New York 10003, USA.
Lukeman
Philip S
PS
Canary
James W
JW
Seeman
Nadrian C
NC
D000478:Alkylation; D004277:DNA, Single-Stranded / Q000138:chemical synthesis* / Q000737:chemistry; D009757:Nylons / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D004277:DNA, Single-Stranded; D009757:Nylons; D009841:Oligonucleotides