title: The relationship between the presence of extra alpha-globin genes and blood cell traits in Altamurana sheep.
abstract: Additional alpha-globin genes in sheep might produce extra alpha-globin chains and, consequently, the subject carrying triplicated (alphaalphaalpha) or quadruplicated (alphaalphaalphaalpha) haplotypes may exhibit different hematological phenotypes when compared to the normal duplicated (alphaalpha) homozygotes (NN). Both alphaalphaalpha and alphaalphaalphaalpha heterozygous (ND) and alphaalphaalpha and alphaalphaalphaalpha homozygous (DD) individuals were obtained by selection and inbreeding. Chromatographic RP-HPLC analyses of the globin chains of 65 subjects (15 DD, 20 ND and 30 NN) were performed. A highly significant linear regression (r2 = 0.967) of the alpha/beta ratio on the number of alpha-globin genes was found, and the alpha/beta ratio ranged on average from 1.0 in NN individuals to 1.2 in the ND and 1.6 in the DD subjects. Values for blood fell within the range of normality but were rather peculiar as a whole. When the erythrocytes of individuals carrying normal arrangements were compared with those of subjects with extra alpha-genes, the latter had fewer erythrocytes that were bigger in size and had a higher Hb content and a greater osmotic fragility. This hematological picture is consistent with the existence of an unbalanced alpha/beta ratio.
authors.affiliation: Department of Engineering and Management of the Agricultural, Livestock and Forest Systems, University of Bari, Via Amendola 165/A, 70100 Bari, Italy. pierelis@agr.uniba.it
authors.forename: Elisa
authors.initials: E
authors.lastname: Pieragostini
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Petazzi
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Luccia
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003433:Crosses, Genetic; D020131:Genes, Duplicate / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006453:Hemoglobinopathies / Q000235:genetics*; D007558:Italy; D011110:Polymorphism, Genetic / Q000235:genetics; D012044:Regression Analysis; D012756:Sheep / Q000097:blood / Q000235:genetics*
chemical_list: D005914:Globins