[Effects of chemokine-like factor 1 (CKLF1) on proliferation and metabolism of chondrocytes].
OBJECTIVE
To investigate the effects of chemokine-like factor 1 (CKLF1) on proliferation and metabolism of chondrocytes.


METHODS
Cell culture, 3H-TdR and 3H-Proline incorporation methods were used. Chondrocytes were harvested from rabbit articular cartilage. First passage cells were seeded on 96-well plates. After synchronization, the medium was replaced by DMEM containing 5% FCS with various concentration of CKLF1 conditioned medium. The synthesis of mucopolysaccharide was detected by Saffraan O staining. The transcription of inducible nitricoxide synthase (iNOS) was detected by semi-quantitative RT-PCR.


RESULTS
CKLF1 inhibited the DNA, collagen and mucopolysaccharide synthesis significantly, meanwhile, stimulated the transcription of iNOS.


CONCLUSIONS
CKLF1 inhibits the proliferation and matrix synthesis of chondrocytes, which might be an important factor resulting in cartilage destructive lesions. CKLF1 may exert its effects on chondrocytes through iNOS pathway.
Cheng
Aixin
A
Department of Orthopaedics Surgery, Peking University Third Hospital, Beijing, China.
Han
Wenling
W
Ma
Dalong
D
Lou
Siquan
S
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D003094:Collagen; D004247:DNA; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011817:Rabbits
D018925:Chemokines; D003094:Collagen; D004247:DNA; D052247:Nitric Oxide Synthase Type II