Antigenic and genetic variability of human respiratory syncytial viruses (group A) isolated in Uruguay and Argentina: 1993-2001.
The antigenic and genetic diversity of G glycoprotein from 25 human respiratory viruses (group A) isolated during nine consecutive epidemics (1993-2001) in Montevideo, Uruguay, and 7 strains isolated in Buenos Aires, Argentina, in the same period were analyzed. Genetic variability was evaluated by partial sequence of the G protein gene. Phylogenetic analysis indicated that most Uruguayan and Argentinean group A isolates clustered into three genotypes: GA5, GA2, and GA1. Some strains clustered into the GA3 genotype characterized previously. The antigenic analysis was carried out with a panel of anti-G monoclonal antibodies that recognized conserved and strain-specific epitopes. A close correlation between the antigenic and genetic relatedness of the strains analyzed was observed.
Frabasile
Sandra
S
Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Delfraro
Adriana
A
Facal
Luján
L
Videla
Cristina
C
Galiano
Mónica
M
de Sierra
María José
MJ
Ruchansky
Dora
D
Vitureira
Nathalia
N
Berois
Mabel
M
Carballal
Guadalupe
G
Russi
José
J
Arbiza
Juan
J
D000595:Amino Acid Sequence; D000940:Antigenic Variation; D000956:Antigens, Viral; D001118:Argentina / Q000453:epidemiology; D002675:Child, Preschool; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014583:Uruguay / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
D000956:Antigens, Viral; D014764:Viral Proteins