Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle.
An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.
Ota
Hiroyuki
H
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Arakaki
Atsushi
A
Tanaka
Tsuyoshi
T
Takeyama
Haruko
H
Matsunaga
Tadashi
T
D001426:Bacterial Proteins / Q000737:chemistry*; D020137:Base Pair Mismatch / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D001426:Bacterial Proteins; D020099:Coated Materials, Biocompatible; D008565:Membrane Proteins; C405425:MpsA protein, Magnetospirillum sp.; D015345:Oligonucleotide Probes