Complete genome sequence of the oral pathogenic Bacterium porphyromonas gingivalis strain W83.
The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.
Nelson
Karen E
KE
The Institute for Genomic Research, Rockville, Maryland 20850, USA. kenelson@tigr.org
Fleischmann
Robert D
RD
DeBoy
Robert T
RT
Paulsen
Ian T
IT
Fouts
Derrick E
DE
Eisen
Jonathan A
JA
Daugherty
Sean C
SC
Dodson
Robert J
RJ
Durkin
A Scott
AS
Gwinn
Michelle
M
Haft
Daniel H
DH
Kolonay
James F
JF
Nelson
William C
WC
Mason
Tanya
T
Tallon
Luke
L
Gray
Jessica
J
Granger
David
D
Tettelin
Hervé
H
Dong
Hong
H
Galvin
Jamie L
JL
Duncan
Margaret J
MJ
Dewhirst
Floyd E
FE
Fraser
Claire M
CM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D016680:Genome, Bacterial; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D010510:Periodontal Diseases / Q000382:microbiology; D010802:Phylogeny; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D006388:Hemagglutinins