Endoscopic laser imaging of tissue perfusion: new instrumentation and technique.
OBJECTIVE
New instrumentation, based on a previously established laser speckle perfusion imaging (LSI) technique is evaluated for its ability to capture and generate blood flow images during endoscopic surgery.


METHODS
Investigations are detailed in an in-vitro blood flow model simulating physiological properties of vascularized tissue, and in-vivo in rabbit joint capsule tissue.


RESULTS
In-vitro measurements showed a linear response of the instrument to blood flow in the range of 0-800 microl/minute, where data points were significantly correlated with an r(2) value of 0.96. In-vivo measurements showed a 58.7% decrease to the medial collateral ligament during occlusion of the femoral artery.


CONCLUSIONS
Blood flow images demonstrate that the endoscopic LSI technique is capable of measuring relative tissue blood flow changes at high resolutions and rapid response times and incorporates well with endoscopic surgeries.
Forrester
K R
KR
McCaig Centre for Joint Injury and Arthritis Research, Department of Surgery, University of Calgary, Calgary, Alberta, Canada T2N 4N1. kforrest@ucalgary.ca
Stewart
C
C
Leonard
C
C
Tulip
J
J
Bray
R C
RC
D000818:Animals; D001182:Arthroscopy / Q000379:methods*; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D017746:Joint Capsule / Q000098:blood supply* / Q000293:injuries; D007719:Knee Joint / Q000098:blood supply*; D007834:Lasers; D008955:Models, Cardiovascular; D011817:Rabbits; D012039:Regional Blood Flow; D015203:Reproducibility of Results