[Spectroscopic studies on the binding of effective component of Chinese herbs with human serum albumin].
Tanshione II A and Cryptotanshinone are the main effective components of the traditional Chinese medicine Salvia miltiorrhiza bung. They both have many kinds of physiologic activities. When a medicine enters the body, it always goes to the acceptor site and has pharmacodynamic action by way of storage and carriage of plasma. Serum albumin is the major serum protein, which can combine with many inner and outer source compounds. In this paper, we have studied the interaction of Tanshinone with the human serum albumin(HSA) in beta-CD buffer system by spectroscopic method. The results show that the absorption maximum of Tanshinone exhibit red shift upon binding to HSA and the absorbance enhance. Tanshinone have stronger fluorescence quenching effect on HSA and the dissociation constants of Tanshinone bound to HSA are calculated. The distance of protein Trp-214 to the first binding site is also calculated on the basis of energy transfer between HSA and bound Tanshinone.
Zhang
L W
LW
Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Yang
P
P
Wang
F
F
D045784:Abietanes; D001665:Binding Sites; D003505:Cyclodextrins; D004735:Energy Transfer; D006801:Humans; D010616:Phenanthrenes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan
D045784:Abietanes; D003505:Cyclodextrins; D010616:Phenanthrenes; D012709:Serum Albumin; C021751:tanshinone; C037886:cryptotanshinone; D014364:Tryptophan