Protein composition and activation markers in plasma collected by three apheresis procedures.
BACKGROUND
Scientific and technical advances made in transfusion medicine sustain the need for more comprehensive understanding of the impact of collection procedures on the quality of plasma for fractionation and for transfusion. This prospective work evaluated protein composition and markers of activation in plasma donations collected with three different automatic collection procedures (performed on Haemonetics machines), including a new procedure using a high-separation core-molded bowl.


METHODS
A total of 90 collection procedures have been performed from a population of 37 donors, under comprehensively standardized conditions. Plasma aliquots were taken from the plasma units within 30 minutes of the end of the collection procedures and immediately frozen at -70 degrees C. Content in an extended range of proteins and of markers of activation of the coagulation and fibrinolytic systems has been measured using standard in vitro testing methods.


RESULTS
Plasma donations had normal mean total protein, IgG, IgM, and fibrinogen content. The mean levels in coagulation FV, FVII, FVIII, and FXI and in antithrombin were above the standard international requirements. There was no sign of activation of the hemostasis system, as assessed by activated FVII, thrombin antithrombin complex, Prothrombin fragment 1+2, and D-dimers. Activated complement component C3 and C5 were low.


CONCLUSIONS
Data indicates the good and consistent protein composition of plasma obtained by those automatic apheresis procedures. In particular, the new high-separation core procedure yields a high-quality plasma meeting requirements for transfusion and fractionation.
Burnouf
Thierry
T
Human Plasma Product Services, Lille, France. tburnou@attglobal.net
Kappelsberger
Claudia
C
Frank
Kerstin
K
Burkhardt
Thomas
T
D000328:Adult; D015415:Biomarkers; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000032:analysis; D001781:Blood Component Removal / Q000379:methods* / Q000592:standards; D016913:Blood Component Transfusion / Q000295:instrumentation*; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D010949:Plasma / Q000737:chemistry*; D011446:Prospective Studies
D015415:Biomarkers; D001779:Blood Coagulation Factors; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005340:Fibrinogen