Usefulness of deoxyribonuclease I (DNase I) polymorphism for individualization from small aged urine stains.
We devised a procedure that combines a simple extraction method, isoelectric focusing and activity staining using the dried agarose film overlay method, for deoxyribonuclease I (DNase I) typing from aged urine stains. DNase I types were determined without difficulty from urine stains kept at room temperature for 3 months or more in all of the samples tested. The amounts of urine stains required for typing after 3 months of storage were estimated to be equivalent to 60-120 microl of liquid urine. Therefore, considering that useful PCR-based DNA typing has not yet been developed for urine stains, DNase I polymorphism could be considered the first biochemical marker found to be well suited for individualization from small aged urine stains.
Yasuda
Toshihiro
T
Department of Biology, Fukui Medical University, 910-1193, Fukui, Japan.
Takeshita
Haruo
H
Ueki
Misuzu
M
Nakajima
Tamiko
T
Mogi
Koichi
K
Kaneko
Yasushi
Y
Iida
Reiko
R
Kishi
Koichiro
K
D002847:Chromatography, Agarose; D016172:DNA Fingerprinting / Q000379:methods; D003850:Deoxyribonuclease I / Q000235:genetics* / Q000652:urine*; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013997:Time Factors
D005819:Genetic Markers; D003850:Deoxyribonuclease I