Role of periplasmic peptidylprolyl isomerases in Salmonella enterica serovar Typhimurium virulence.
FkpA is a peptidylprolyl isomerase whose expression is regulated by the alternative sigma factor, sigma factor E (sigma(E)). In contrast to the results of a previous report, inactivation of fkpA was found to have only a minor effect on the ability of Salmonella enterica serovar Typhimurium to invade and survive within epithelial and macrophage cell lines and cause infection in mice. However, an effect of the fkpA mutation on serovar Typhimurium virulence was seen if the mutation was combined with mutations in surA or htrA, two other sigma(E)-regulated genes, which encode proteins involved in protein folding and/or degradation in the periplasm.
Humphreys
Sue
S
Molecular Bacteriology Group, Institute of Comparative Medicine, Department of Veterinary Pathology, Glasgow University Veterinary School, Glasgow G61 1QH, United Kingdom.
Rowley
Gary
G
Stevenson
Andrew
A
Kenyon
William J
WJ
Spector
Michael P
MP
Roberts
Mark
M
D000818:Animals; D018938:Caco-2 Cells; D002352:Carrier Proteins; D002460:Cell Line; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000502:physiology; D006801:Humans; D020104:Immunophilins / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009154:Mutation; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000502:physiology*; D033903:Periplasmic Proteins / Q000502:physiology; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D012697:Serine Endopeptidases / Q000502:physiology; D012808:Sigma Factor / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014774:Virulence / Q000235:genetics / Q000502:physiology
D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D033903:Periplasmic Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors; C061682:DegP protease; D012697:Serine Endopeptidases; C098640:periplasmic peptidylprolyl cis-trans isomerase; D020104:Immunophilins; D019696:Peptidylprolyl Isomerase