title: Two STR loci polymorphisms of adenosine deaminase gene in Japanese population.
abstract: Polymorphism of two tetranucleotide tandem repeat loci in the adenosine deaminase (ADA) gene was studied in a Japanese population. A TAAA repeat at approximately 1100 nucleotide 5' to the ADA transcription initiation site, and a TTTA repeat within intron 3 of the ADA gene were characterized using the polymerase chain reaction (PCR) and sequence analysis. Among the 235 individuals studied, seventeen genotypes at the ADA-TAAA locus and ten genotypes at the ADA-TTTA locus were identified, yielding discrimination potentials of 0.90 and 0.66, respectively. The observed and expected genotype values are consistent with Hardy-Weinberg equilibrium. The allele frequencies in the Japanese population were compared with the reported values for other populations. At the ADA-TAAA locus, there were significant differences between the Japanese population and an American Caucasian sample. At the ADA-TTTA locus, no significant differences were observed between Japanese and Chinese or between Japanese and Basques, but significant differences were found between our Japanese population and Italians and Mossi.
authors.affiliation: Department of Legal Medicine, Nihon University School of Medicine, Tokyo, Japan. jtie@med.nihon-u.ac.jp
authors.forename: Jian
authors.initials: J
authors.lastname: Tie
authors.forename: Yuka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Shojiro
authors.initials: S
authors.lastname: Tsukamoto
authors.forename: Shigemi
authors.initials: S
authors.lastname: Oshida