FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers.
Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short hybridization time and even with the ability to hybridize without prior thermal denaturation of the template.
Silahtaroglu
Asli N
AN
Wilhelm Johannsen Centre for Functional Genome Research, Department of Medical Genetics, IMBG, The Panum Institute, University of Copenhagen, Building 24.4, Blegdamsvej 3, 2200, Copenhagen N, Denmark. asli@medgen.ku.dk
Tommerup
Niels
N
Vissing
Henrik
H
D015342:DNA Probes; D018076:DNA, Complementary; D004276:DNA, Satellite / Q000032:analysis*; D023281:Genomics / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013696:Temperature; D013997:Time Factors
D015342:DNA Probes; D018076:DNA, Complementary; D004276:DNA, Satellite; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid