Short-term effect of epidermal growth factor on glucose uptake in endoscopic biopsies.
Epidermal growth factor (EGF) up-regulation of glucose absorption via increased Na+/glucose co-transporter (SGLT-1) activity has previously been described in rabbit jejunal brush-border membrane and in differentiated Caco-2 cells. The goal of the present study was to assess the in vitro effect of EGF (200 ng/ml) on glucose uptake in human mucosal specimens, and we describe a simple procedure that uses endoscopic biopsies for short-term gludose uptake measurements. Uptake values for the EGF-treated biopsies ranged from 2.7 to 29.0, with a mean uptake of 10.65, while uptake values for the untreated biopsies ranged from 0.9 to 17.5, with a mean uptake of 7.99 (P < 0.05, paired t test). This early effect of EGF on human enterocytes may have important therapeutic implications. A role in increasing the rate of internal rehydration is suggested.
Tonb
Dalal
D
Alfred I. duPont Hospital for Children, Wilmington, Delaware 19899, USA.
Mehta
Raj
R
Wang
Hugh
H
Tung
John
J
Mehta
Devendra I
DI
D000293:Adolescent; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques; D016099:Endoscopy, Gastrointestinal; D020895:Enterocytes / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000502:physiology; D008757:Methylglucosides / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D051269:Sodium-Glucose Transporter 1; D014882:Water-Electrolyte Balance / Q000187:drug effects
D008562:Membrane Glycoproteins; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; C027020:methylglucoside; D004815:Epidermal Growth Factor; D005947:Glucose