title: DNA damage in digestive gland and mantle tissue of the mussel Perna perna.
abstract: Data concerning the susceptibility of DNA to damage by reactive oxygen and nitrogen species and other endogenous compounds produced by physiological stress in marine organisms is lacking, especially in bivalve mollusks. In this article, we analyzed the background levels of lipid peroxidation (malondialdehyde, MDA), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-epsilon dGuo) in digestive gland and mantle tissue of mussels Perna perna collected at a cultivation zone in Florianópolis (Santa Catarina, Brazil). The present data point to the possibility of the use of both 8-oxodGuo and 1,N2-epsilon dGuo as complementary indicators of oxidative stress processes in mussels. A sensitive method coupling high performance liquid chromatography to mass spectrometry was applied for the detection of 1,N2-epsilon dGuo in mussel tissues.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26.077, Av. Prof. Lineu Prestes 748, São Paulo, SP, CEP 05513-970, Brazil.
authors.forename: Eduardo Alves
authors.initials: EA
authors.lastname: de Almeida
authors.forename: Sabrina de Almeida
authors.initials: Sde A
authors.lastname: Marques
authors.forename: Clécio Fernando
authors.initials: CF
authors.lastname: Klitzke
authors.forename: Afonso Celso Dias
authors.initials: AC
authors.lastname: Bainy
authors.forename: Marisa Helena Gennari
authors.initials: MH
authors.lastname: de Medeiros
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Mascio
authors.forename: Ana Paula de Melo
authors.initials: AP
authors.lastname: Loureiro
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species