title: [HLA-A genotyping by oligonucleotide arrays].
abstract: OBJECTIVE
  To investigate HLA genotyping by oligonucleotide arrays.
  
  
  METHODS
  Unsymmetrical PCR was used to amplify HLA-A gene exon 2, 3. The PCR products were used as templates for hybridization. The oligonucleotide probes were spotted on glass to make microarrays. High signal and specific probes were selected. The effects of the length and location of probes on hybridization signal were studied. A set of computer software was designed for scanning and genotype differentiation.
  
  
  RESULTS
  The genotypes of 30 samples analyzed by microarray showed concordance to that by SBT and PCR-SSP.
  
  
  CONCLUSIONS
  HLA-A genotyping by oligonucleotide array is a good method with advantage of high speed, low cost and high flux.
authors.affiliation: Shenzhen Yishengtang Biological Products Co, LTD, Shenzhen 518026, China.
authors.forename: Shou-wang
authors.initials: SW
authors.lastname: Hu
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yong-yao
authors.initials: YY
authors.lastname: Geng
authors.forename: Sheng-qi
authors.initials: SQ
authors.lastname: Wang
mesh_terms: D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
chemical_list: D015234:HLA-A Antigens; D015345:Oligonucleotide Probes