title: [Effect of shenfu injection on the synthesis of pulmonary surfactant in cultured lung explants].
abstract: OBJECTIVE
  To explore the effect of shenfu injection on pulmonary surfactant synthesis.
  
  
  METHODS
  Lung explants of adult rats were cultured in free serum DMEM. [3H] choline incorporated into lung tissue was determined by liquid scintillation system. The contents of total phospholipid (TPL) and phosphatidylcholine (PC) in lung tissue were measured by inorganic phosphorus quantitative analysis and thin-layer chromatography, respectively.
  
  
  RESULTS
  Shenfu injection enhanced [3H] choline incorporation in a dose-dependent manner and increased the contents of TPL and PC as well as the ratio of PC/TPL. Both protein kinase C (PKC) antagonist H7 and calmodulin antagonist W7 inhibited the effects of Shenfu injection. The ginsenosides, ginsenoside Rb1, and ginsenoside Rg1 promoted the [3H] choline incorporation.
  
  
  CONCLUSIONS
  Shenfu injection can enhance the synthesis of pulmonary surfactant through pathways mediated by PKC and calmodulin. The ginsenoside is an effective component in Shenfu injection.
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Zi-qiang
authors.initials: ZQ
authors.lastname: Luo
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Feng
authors.forename: Shao-jie
authors.initials: SJ
authors.lastname: Yue
mesh_terms: D031841:Aconitum; D000818:Animals; D046508:Culture Techniques; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D008168:Lung / Q000378:metabolism*; D008297:Male; D005894:Panax; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; C035054:ginsenoside Rg1