[Expression of nm23 genes in acute myeloid leukemia and its clinical significance].
OBJECTIVE
To explore nm23 gene mRNA expression and its clinical significance in acute leukemias (AML).


METHODS
The levels of nm23-H1 and nm23-H2 transcripts in 22 patients with acute myeloid leukemia (AML), 9 AML in complete remission (AML-CR), 12 acute lymphoblastic leukemia (ALL) and 4 chronic myeloid leukemia in chronic phase (CML-CP) were assayed by reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
The expression of nm23-H1 in AL especially in AML-M4 and AML-M5 was significantly higher than that in normal blood cells. An analysis of correlation between nm23 expression and clinicopathological parameters showed that increased nm23-H1 mRNA levels were associated with some poor-prognostic factors such as extramedullary infiltration, high white blood cell count (WBC), high lactate dehydrogenase (LDH) activity and high CD(7) expression, while inversely correlated with t(8; 21) and t(15; 17) which had a good-prognostic effect. The expression of nm23-H1 in AML patients in CR was significantly decreased compared with those untreated.


CONCLUSIONS
nm23-H1 was overexpressed in AL, especially in AML-M4 and AML-M5. High expression of nm23-H1 may be a poor prognostic factor.
Meng
Qing-xiang
QX
Peking University Shenzhen Hospital, Shenzhen 518036, China.
Jiang
Rong
R
Yang
Bao-qing
BQ
Zhang
Hong-yu
HY
Liu
Jin
J
Pang
Li-ping
LP
Wang
Jun
J
D000328:Adult; D005260:Female; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase