Presence of calcitonin receptors in shell gland of the guineafowl and changes in binding property during an oviposition cycle.
Radioligand binding assays of membrane fraction of the shell gland endometrium of guineafowl revealed the presence of receptor for calcitonin. The equilibrium dissociation constant (Kd) obtained by Scatchard analyses was 0.50 to 1.25 nM in laying birds and 1.12 to 1.19 nM in nonlaying birds. The maximum binding capacity (Bmax) per milligram of protein was 33.1 to 107.5 femtomoles in laying birds and 101.4 to 114.9 femtomoles in nonlaying birds. Both Kd and Bmax values changed during eggshell formation in laying birds. The results suggest that calcitonin receptor binding may be related to eggshell formation in guineafowl.
Ogawa
Hiroshi
H
Department of Animal Science, Tokyo University of Agriculture, Atsugi-shi, Kanagawa 243-0034, Japan.
Takahashi
Tetsuya
T
Kuwayama
Takehito
T
Kawashima
Mitsuo
M
D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D004528:Egg Shell; D005088:Exocrine Glands / Q000737:chemistry*; D005260:Female; D007457:Iodine Radioisotopes; D007700:Kinetics; D010058:Oviposition / Q000502:physiology*; D011200:Poultry / Q000378:metabolism*; D018003:Receptors, Calcitonin / Q000032:analysis* / Q000378:metabolism*
D007457:Iodine Radioisotopes; D018003:Receptors, Calcitonin; D002116:Calcitonin