Intranasal immunization of humans with Streptococcus mutans antigens.
To evaluate the effectiveness of a low dose of soluble or liposomal (L) glucosyltransferase-enriched preparation (E-GTF) in inducing mucosal immune responses after intranasal immunization, 12 adults were immunized on days 0 and 7 by the IN route with 62.5 microg of soluble E-GTF or L-E-GTF. An increase in the mean salivary IgA anti-E-GTF response (P < 0.03) was seen in the L-E-GTF but not the soluble E-GTF group. A significant increase (P < 0.05) in the mean specific IgA antibody activity was also seen in nasal wash from both groups. Although the nasal wash responses were higher in the L-E-GTF than in the soluble E-GTF group, they were not significantly different. The soluble E-GTF immunized group showed a higher serum IgG response than the L-E-GTF immunized group on day 90 (P < 0.05). These results indicate that as little as 62.5 microg of E-GTF, when given by the intranasal route, induced an IgA response in secretions.
Li
F
F
Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, AL 35294-0007, USA.
Michalek
S M
SM
Dasanayake
A P
AP
Li
Y
Y
Kirk
K
K
Childers
N K
NK
D000281:Administration, Intranasal; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000627:therapeutic use*; D005964:Glucosyltransferases / Q000008:administration & dosage / Q000627:therapeutic use; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D008081:Liposomes; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000276:immunology; D012995:Solubility; D013295:Streptococcus mutans / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D008081:Liposomes; C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases