[Effect of pingyangmycin on the growth and cell cycle of human vascular endothelial cells].
OBJECTIVE
To study the effect of pingyangmycin (PYM, bleomycin A5) on the proliferation and cell cycle of the cultured ECV304 cells, a human umbilical vein endothelial cell (HUVEC) line.


METHODS
The growth inhibition of PYM on ECV304 cells was measured by MTT assay and the changes in the cell cycle by flow cytometry.


RESULTS
After 10 microg/ml PYM treatment of the cells for 24, 48, and 72 h, the inhibition rates were 44.7%, 59.7%, and 74.4% respectively, showing a dose- and time-dependent inhibitory effect, with the 50% inhibitory concentration (IC50) of PYM corresponding to treatment durations of 24, 48 and 72 h being 32.94, 2.56 and 0.75 microg/ml respectively. Flow cytometry showed that ECV304 cell cycle was arrested at G2-M phase after 24-hour treatment with 1 microg/ml PYM, with significant reduction in the cell ratio of S phase and increase of G2-M phase (P<0.01)


CONCLUSIONS
PYM can effectively inhibit the proliferation of ECV304 cells, the mechanism of which might involve the blocking of cell cycle.
Kong
Wei-dong
WD
Department of Interventional Radiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. kongwd@msn.com
Li
Yan-hao
YH
Zeng
Qing-le
QL
Chen
Yong
Y
He
Xiao-feng
XF
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C021771:zhengguangmycin