title: Molecular evidence for transferrin receptor 2 expression in all FAB subtypes of acute myeloid leukemia.
abstract: We examined transferrin receptor (TfR) 1 and TfR2 mRNA expression in 50 acute myeloid leukemia (AML) patients by RT-PCR, with primers specific for exons 15-17 (TfR1), 3-5 (TfR2-alpha) and 4-5 (TfR2-beta) of the corresponding gene. There were 4/50 TfR1-negative (-), 3/50 TfR2-alpha mRNA (-) and 13/50 TfR2-beta mRNA (-) cases; only three cases were TfR1/2 mRNA (-). No significant correlations were identified between TfR2-beta mRNA negativity and specific FAB subtypes, karyotype or attainment of complete remission. These findings suggest that: (i) TfR2 expression is not restricted to erythroid cells, and (ii) iron import proteins might complement each other in AML cells.
authors.affiliation: Department of Biology, University of Thessaly, Larissa, Greece. kollia@med.uth.gr
authors.forename: Panagoula
authors.initials: P
authors.lastname: Kollia
authors.forename: Maria
authors.initials: M
authors.lastname: Samara
authors.forename: Kostas
authors.initials: K
authors.lastname: Stamatopoulos
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Belessi
authors.forename: Niki
authors.initials: N
authors.lastname: Stavroyianni
authors.forename: Aliki
authors.initials: A
authors.lastname: Tsompanakou
authors.forename: Anastasia
authors.initials: A
authors.lastname: Athanasiadou
authors.forename: Nikos
authors.initials: N
authors.lastname: Vamvakopoulos
authors.forename: Nikos
authors.initials: N
authors.lastname: Laoutaris
authors.forename: Achilles
authors.initials: A
authors.lastname: Anagnostopoulos
authors.forename: Athanasios
authors.initials: A
authors.lastname: Fassas
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics*; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D007140:Immunoglobulin Fab Fragments; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011990:Receptors, Transferrin; C503640:TFR2 protein, human; D007501:Iron