[Survivin antisense RNA enhances taxol-induced apoptosis in leukemia cell line HL-60].
OBJECTIVE
To explore the effects of survivin antisense RNA on taxol-induced apoptosis in leukemia cell line HL-60.


METHODS
A survivin antisense eukaryotic vector pcDNA3-SVVas was transferred into HL-60 cells by electroporation. The live fraction was determined by trypan blue dye exclusion assay. Cell counting and MTT assay were performed to evaluate the sensibility of the transfected cells to taxol. Apoptosis was detected by DNA gel electrophoresis and nuclear staining.


RESULTS
Two positive cell clones, HL-60 SVVas and HL-60 neo were obtained. Compared to HL-60 and HL-60 neo cells, HL-60 SVVas cells growth was significantly reduced (P < 0.05). By MTT assay, the IC(50) of taxol to HL-60 SVVas, HL-60 neo and HL-60 cells were (14.4 +/- 1.87) ng/ml, (31.9 +/- 6.38) ng/ml and (32.0 +/- 3.52) ng/ml, respectively, the difference was significant by statistic analysis (P < 0.01). Agarose gel electrophoresis of genomic DNA from HL-60 SVVas showed typical DNA ladder, but DNA from HL-60 neo and HL-60 did not. Nuclei become condense in HL-60 SVVas cells.


CONCLUSIONS
Survivin antisense RNA could enhance taxol-induced apoptosis in leukemia cell line HL-60. This may lay an experimental foundation for further research of gene therapy in leukemia.
Wang
Xiao-juan
XJ
Department of Immunology, Tongji Medical College, Huazhong University of Science and Techonology, Wuhan 430030, China.
Dai
Guo-yi
GY
Cao
Li-min
LM
Zhu
Hui-fen
HF
Zhang
Yue
Y
Shao
Jing-fang
JF
Yang
Jing
J
Shen
Guan-xin
GX
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004357:Drug Synergism; D018922:HL-60 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D017239:Paclitaxel / Q000494:pharmacology*; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D000077022:Survivin; D014162:Transfection
D000972:Antineoplastic Agents, Phytogenic; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D016372:RNA, Antisense; D000077022:Survivin; D017239:Paclitaxel