Stereospecific high-performance liquid chromatographic analysis of flurbiprofen: application to pharmacokinetic studies.
A method of analysis of flurbiprofen (+/- 2-(2-fluoro-4-biphenyl)-propionic acid) in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and tissue distribution. A simple high-performance liquid chromatographic method was developed for simultaneous determination of flurbiprofen enantiomers in rat serum. Serum (0.1 ml) was extracted with 2,2,4-trimethylpentane-isopropanol (95:5, v/v) after addition of the internal standard (IS), S-naproxen and acidification with H(2)SO(4). Separation was achieved on a Chiralpak AD-RH column with UV detection at 247 nm. The calibration curve was linear ranging from 0.05 to 50 microg/ml for each enantiomer. The mean extraction efficiency was >95%. Precision of the assay was <11% (CV), and was within 12.6% at the limit of quantitation (LOQ) (0.05 microg/ml). Bias of the assay was lower than 13.1%, and was within 12.8% at the LOQ. The assay was applied successfully to the in vivo kinetic study of flurbiprofen in rats.
Teng
Xiao Wei
XW
Department of Pharmaceutical Sciences and Pharmacology, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA.
Wang
Stephen W J
SW
Davies
Neal M
NM
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D005480:Flurbiprofen / Q000032:analysis* / Q000493:pharmacokinetics*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D012996:Solutions; D005480:Flurbiprofen