title: Allosteric effect of water in fish and human hemoglobins.
abstract: Prompted by the reported lack of solvation effects on the oxygen affinity of fish (trout I) hemoglobin that questioned allosteric water binding in human hemoglobin A (Bellelli, A., Brancaccio, A., and Brunori, M. (1993) J. Biol. Chem. 268, 4742-4744), we have investigated solvation effects in fish and human hemoglobins by means of the osmotic stress method and allosteric analysis. In contrast to the earlier report, we demonstrate that water potential does affect oxygen affinity of trout hemoglobin I in the presence of inert solutes like betaine. Moreover, we show that upon oxygenation electrophoretically anodic hemoglobin from trout and eel bind a similar number of water molecules as does human hemoglobin A, whereas the cathodic hemoglobins of trout and eel bind smaller, but mutually similar, numbers of water molecules. Addition of cofactors strongly increases the number of water molecules bound to eel hemoglobin A (as in human hemoglobin) but only weakly affects water binding to eel hemoglobin C.
authors.affiliation: Department of Zoophysiology, Institute of Biological Sciences, C. F. Møllers Alle 131, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Christian
authors.initials: C
authors.lastname: Hundahl
authors.forename: Angela
authors.initials: A
authors.lastname: Fago
authors.forename: Hans
authors.initials: H
authors.lastname: Malte
authors.forename: Roy E
authors.initials: RE
authors.lastname: Weber
mesh_terms: D000495:Allosteric Site; D000818:Animals; D001622:Betaine / Q000737:chemistry; D004524:Eels; D006441:Hemoglobin A / Q000737:chemistry; D006444:Hemoglobin C / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008956:Models, Chemical; D009995:Osmosis; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013045:Species Specificity; D013696:Temperature; D014337:Trout; D014867:Water / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D014867:Water; D001622:Betaine; D006444:Hemoglobin C; D006441:Hemoglobin A; D010100:Oxygen