Effect of intracarotid administration of adrenomedullin on the spontaneous electrical activity of area postrema neurons in sino-aortic denervated rats.
To observe the effect of intracarotid administration of adrenomedullin (AM) on the spontaneous electrical activity of area postrema (AP) neurons, 78 spontaneous active units were recorded from 63 sino-aortic denervated Sprague-Dawley rats using extracellular recording technique. The results obtained are as follows. (1) Following intracarotid administration of AM (0.3 nmol/kg), the discharge rate of 47 out of 78 units increased markedly from 2.99+/-0.24 to 4.79+/-0.29 spikes/s (P<0.001), 20 units decreased from 3.24+/-0.46 to 1.97+/-0.37 spikes/s (P<0.001), and the remaining 11 showed no response. Blood pressure (BP) and heart rate (HR) did not change throughout the experimentation. (2) Pretreatment with intracarotid administration of calcitonin gene-related peptide receptor antagonist CGRP8-37 (3 nmol/kg) did not change the effects of AM. (3) Following intracarotid injection of NO precursor L-arginine (30 mg/kg), the excitatory effect of AM was attenuated. The above results indicate that AM can excite spontaneous electrical activity of AP neurons, this effect is not mediated by calcitonin gene-related peptide receptor but may be attenuated by NO precursor L-arginine.
Ji
Shu-Mei
SM
Department of Physiology, Hebei Medical University, Shijazhuang 050017.
Yue
Hua
H
He
Rui-Rong
RR
D000200:Action Potentials / Q000502:physiology*; D053607:Adrenomedullin / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000294:innervation* / Q000502:physiology / Q000601:surgery; D031608:Area Postrema / Q000502:physiology*; D002346:Carotid Sinus / Q000294:innervation* / Q000502:physiology / Q000601:surgery; D003714:Denervation; D007269:Injections, Intra-Arterial; D008297:Male; D009474:Neurons / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D053607:Adrenomedullin