title: [The synthesis of MT in rat myocardial fibroblasts].
abstract: OBJECTIVE
  To study the influence on the synthesis of metallothionein (MT) induced by lipopolysaccharide (LPS), basic fibroblast growth factor (bFGF), oxidized low-density lipoproteins (oxLDL) and ZnCl2 in rat myocardial fibroblasts.
  
  
  METHODS
  The content of MT was measured by 109Cd-Hb saturation method and RT-PCR was used to determine the expression of MT mRNA.
  
  
  RESULTS
  All of the stimulus in this experiment increased the synthesis of MT in myocardial fibroblasts. Compared with control, 1 and 5 mg.L-1 LPS increased the content of MT by 94% and 130% (P < 0.01), 0.2 and 1.0 mg.L-1 bFGF by 41% and 59% (P < 0.01), 2 and 10 mg.L-1 oxLDL by 23% (P < 0.05) and 41% (P < 0.01) respectively. 1,2 and 5 mg.L-1 ZnCl2 increased the content of MT by 66%, 99% and 149% (P < 0.01) in a concentration-dependent manner. 2-8 mg.L-1 ZnCl2 increased the content of MT1 mRNA by 69%-129%, MT2 mRNA by 31%-60% (P < 0.01). But 10 mg.L-1 ZnCl2 didn't stimulate the synthesis of MT more significantly, probably because ZnCl2 of high concentration damaged myocardial fibroblasts and decreased the survival rate of the cells.
  
  
  CONCLUSIONS
  Many stimulus such as LPS, bFGF, oxLDL and ZnCl2 can induce the synthesis of MT, and ZnCl2 can increase the expression of MT mRNA in rat myocardial fibroblasts.
authors.affiliation: Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.forename: Dongyan
authors.initials: D
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Shi
authors.forename: Dingfang
authors.initials: D
authors.lastname: Bu
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Pang
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Tang
mesh_terms: D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008668:Metallothionein / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D016222:Fibroblast Growth Factor 2; D008668:Metallothionein