title: [Design of primers to DNA of lactic acid bacteria].
abstract: Primers LP1-LP2 to the gene 16S rRNA have been developed, which permit to differentiate lactic acid bacteria: Lactobacillus plantarum, L. delbrueckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus. The strain-specific and species-specific differentiations are possible under different annealing temperature. Additional fragments, which are synthesized outside the framework of gene 16S rRNA reading, provide for the strain-specific type of differentiation, and the fragment F864 read in the gene 16S rRNA permits identifying L. plantarum.
authors.affiliation: Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Lashchevskiĭ
authors.forename: N K
authors.initials: NK
authors.lastname: Kovalenko
mesh_terms: D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S