title: Absence of Chlamydia pneumoniae in brain of vascular dementia patients.
abstract: We recently detected cytomegalovirus (CMV) in brains of 83% of vascular dementia (VaD) patients and 34% of age-matched normal people. Since CMV and also Chlamydia pneumoniae (Cpn) have been found in some studies to be associated with coronary artery disease (which shares several risk factors with VaD), we sought Cpn DNA in VaD brain DNA. We examined brain specimens from 19 VaD patients, 16 elderly normal people and four Alzheimer's disease (AD) patients for the presence of a sequence in the Cpn gene for rRNA, using polymerase chain reaction (PCR) and taking stringent precautions against contamination. We did not detect Cpn DNA in any of the brain specimens, the sensitivity of detection being 10 copies or fewer bacterial DNA sequences per tube or, in terms of infectious units (IFU), 0.025 IFU. Our results do not support a role for Cpn in the aetiology of VaD, either in the 83% of patients in whose brains we detected CMV, or in the remaining 17% without CMV in brain.
authors.affiliation: Department of Optometry and Neuroscience, Molecular Neurobiology Laboratory, University of Manchester Institute of Science & Technology, Manchester M60 1QD, UK.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Wozniak
authors.forename: Ann
authors.initials: A
authors.lastname: Cookson
authors.forename: Gordon K
authors.initials: GK
authors.lastname: Wilcock
authors.forename: Ruth F
authors.initials: RF
authors.lastname: Itzhaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000382:microbiology; D001921:Brain / Q000382:microbiology*; D002690:Chlamydia Infections / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D015140:Dementia, Vascular / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D016555:Matched-Pair Analysis; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012016:Reference Values
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral