title: Oral administration of a turmeric extract inhibits erythrocyte and liver microsome membrane oxidation in rabbits fed with an atherogenic diet.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of an oral supplementation with a Curcuma longa ethanol and aqueous extract on the susceptibility to oxidation of cellular and subcellular membranes affected in the atherosclerotic process, such as erythrocyte membranes and liver microsomes, in rabbits fed with a high-fat diet.
  
  
  METHODS
  Twenty-four male rabbits were randomly assigned to one of two groups: group T was treated with a turmeric hydroalcoholic extract (1.66 mg/kg of body weight) dissolved in a hydroalcoholic mixture vehicle (7:2), and group C (control): received a curcuma-free hydroalcoholic solution (7:2). All rabbits had access ad libitum to 150 g/d of an experimental diet rich in cholesterol and lard to provoke an atherosclerotic process. Erythrocyte membranes and liver microsomes were isolated, and the levels of hydroperoxides and thiobarbituric acid-reactive substances were measured after oxidation induction.
  
  
  RESULTS
  The oxidation of erythrocyte membranes in group T was significantly lower than that in group C, mainly by 30 d (P < 0.05). Levels of hydroperoxides and thiobarbituric acid-reactive substances in liver microsomes also were significantly lower in group T than in group C (P < 0.05).
  
  
  CONCLUSIONS
  The results of this study indicated that oral administration of a nutritional dose of C. longa extracts reduces the susceptibility to oxidation of erythrocyte and liver microsome membranes in vitro and may contribute to the prevention of effects caused by a diet high in fat and cholesterol in blood and liver during the development of atherosclerosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Granada, Spain.
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Mesa
authors.forename: Concepción M
authors.initials: CM
authors.lastname: Aguilera
authors.forename: Cesar L
authors.initials: CL
authors.lastname: Ramírez-Tortosa
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Ramírez-Tortosa
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.forename: Luis
authors.initials: L
authors.lastname: Baró
authors.forename: Emilio
authors.initials: E
authors.lastname: Martínez de Victoria
authors.forename: Angel
authors.initials: A
authors.lastname: Gil
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001161:Arteriosclerosis / Q000378:metabolism*; D030024:Curcuma; D004036:Diet, Atherogenic; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D011817:Rabbits; D011897:Random Allocation; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C012596:turmeric extract; D006861:Hydrogen Peroxide