Neuropeptide FF receptors 1 and 2 exert an anti-opioid activity in acutely dissociated rat dorsal raphe and periventricular hypothalamic neurones.
We measured the reduction by nociceptin of the [Ca(2+)](i) transient triggered by depolarization in acutely dissociated neurones of the rat dorsal raphe and periventricular hypothalamic nuclei that express NPFF(2) and NPFF(1) receptors, respectively, in the absence and presence of 10 nM of NPA-NPFF or NPVF, two peptides selective for NPFF(2) and NPFF(1) receptors, respectively. In dorsal raphe neurones, NPA-NPFF reduces the inhibition of Ca(2+) conductances by nociceptin while NPVF is inactive. In periventricular hypothalamic neurones, both peptides reduce the inhibition of Ca(2+) transients by nociceptin, NPVF having a significantly larger effect than NPA-NPFF. These results demonstrate that activation of both NPFF(1) and NPFF(2) receptors has the same cellular anti-opioid effect.
Roumy
Michel
M
Institut de Pharmacologie et de Biologie Structurale (CNRS, UMR 5089), 205 route de Narbonne, 31077 Cedex 04, Toulouse, France. michel.roumy@ipbs.fr
Garnier
Marielle
M
Zajac
Jean-Marie
JM
D000818:Animals; D006801:Humans; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018847:Opioid Peptides / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism*; D011903:Raphe Nuclei / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018013:Receptors, Neuropeptide / Q000819:agonists / Q000378:metabolism*; D000097629:Nociceptin
D018847:Opioid Peptides; D018013:Receptors, Neuropeptide; D000097629:Nociceptin; C083929:neuropeptide FF receptor