title: Molecular epidemiology of human immunodeficiency virus-1 in the state of Ceará, Northeast, Brazil.
abstract: We analyzed, by env and gag heteroduplex mobility assay, 149 human immunodeficiency virus (HIV-1) positive samples collected in Ceará during the year 2000. The prevalence of subtype B was 81.2% and the prevalence of subtype F and B/F recombinants were both 2.7%. Eight (5.4%) and 12 (8%) out of 149 samples showed indeterminate results in the env and gag analysis respectively. By FokI restriction fragment length polymorphism, 34% of the subtype B samples were identified as the typical Brazilian subtype B. In the present study, we identified HIV-1 subtype F and B/F in Ceará for the first time. Our results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates.
authors.affiliation: Laboratório Avançado de Saúde Pública, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, 40295-001, Brasil.
authors.forename: Sandra Rocha
authors.initials: SR
authors.lastname: Gadelha
authors.forename: Nice
authors.initials: N
authors.lastname: Shindo
authors.forename: José Napoleão Monte
authors.initials: JN
authors.lastname: Cruz
authors.forename: Mariza Gonçalves
authors.initials: MG
authors.lastname: Morgado
authors.forename: Bernardo
authors.initials: B
authors.lastname: Galvão-Castro
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D015752:Genes, env / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000453:epidemiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence
chemical_list: D004279:DNA, Viral