Intracellular visualization of prostate cancer using magnetic resonance imaging.
The term "molecular imaging" can be broadly defined as the in vivo characterization and measurement of biological processes at the cellular and molecular level. Is a gene expression magnetic resonance imaging (MRI) possible? Therefore, we have developed a novel intravital and intracellular MRI contrast agent composed of a gadolinium complex, an oligonucleotide sequence [peptide nucleic acid (PNA)], and a transmembrane carrier peptide that is composed of a peptide sequence similar to that of the homeodomain of the Antennapedia protein. The goal of our study was to determine whether this contrast agent could be accumulated in tumor cells in vitro (HeLa cells) and in vivo (Dunning R3327 AT1 rat prostate adenocarcinoma) and whether the specificity of the PNA for the up-regulated c-myc mRNA in the cell's cytoplasm would have an effect on contrast agent retention in the tumor cells. Using the c-myc-specific and a c-myc-nonspecific control PNA, an increase in signal intensity in the tumor cells was observed after 10 min in vitro and in vivo (maximum was reached in HeLa cells in vitro in 60 min, in Dunning R3327 AT1 rat prostate adenocarcinoma cells in vivo in 30 min). This increase of signal intensity could be maintained in vitro in HeLa cells for only 4 h and in Dunning R3327 AT1 rat prostate adenocarcinoma cells in vivo at least for 5 h by using the c-myc mRNA-specific PNA as a "retention" agent.
Heckl
Stefan
S
Department of Neuroradiology, University of Tübingen Medical School, Germany.
Pipkorn
Rüdiger
R
Waldeck
Waldemar
W
Spring
Herbert
H
Jenne
Jürgen
J
von der Lieth
Claus-W
CW
Corban-Wilhelm
Heike
H
Debus
Jürgen
J
Braun
Klaus
K
D000230:Adenocarcinoma / Q000175:diagnosis*; D000818:Animals; D016259:Genes, myc; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D020135:Peptide Nucleic Acids; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics; D051381:Rats
D020135:Peptide Nucleic Acids