title: Plasmodium falciparum: diversity studies of isolates from two Colombian regions with different endemicity.
abstract: The population structure of Plasmodium falciparum has been widely studied in diverse epidemiological contexts, but emphasis has been made in regions with high and stable transmission. In order to establish the genetic structure of P. falciparum in areas of Colombia with different degree of endemicity, we studied 100 samples from malaria patients of two different municipalities. The frequency of multiclonal infection in these areas and the correlation with the endemicity were carried out by comparison of the amplified products from polymorphic segments of MSP-1, MSP-2, and GLURP genes. We found low size polymorphism of the studied genes: 1 MSP-1 allele, 3 MSP-2 alleles, and 4 GLURP alleles. We conclude that the P. falciparum population in the regions studied is genetically homogeneous.
authors.affiliation: Grupo Malaria, Laboratorio de Hemoparásitos, Faculty of Medicine, University of Antioquia, Carrera 51D # 62-29, AA:1226, Medellín, Colombia.
authors.forename: L
authors.initials: L
authors.lastname: Montoya
authors.forename: A
authors.initials: A
authors.lastname: Maestre
authors.forename: J
authors.initials: J
authors.lastname: Carmona
authors.forename: D
authors.initials: D
authors.lastname: Lopes
authors.forename: V
authors.initials: V
authors.lastname: Do Rosario
authors.forename: S
authors.initials: S
authors.lastname: Blair
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002648:Child; D003105:Colombia / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics*; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics; D012424:Rural Population
chemical_list: D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium; C072492:glutamate-rich protein, Plasmodium