Aldosterone regulation of T-type calcium channels.
Voltage-operated calcium channels play a crucial role in signal transduction in many excitable and non-excitable cell types. While a rapid modulation of their activity by hormone-activated kinases and/or G proteins has been recognized for a long time, a sustained control of their expression level has been only recently demonstrated. In adrenal H295R cells, for example, aldosterone treatment selectively increased low threshold T-type calcium current density without affecting L-type currents. Antagonizing the mineralocorticoid receptor (MR) with spironolactone prevented aldosterone action on T-type currents. By RT-PCR, we detected in these cells the presence of two different isoforms of L-type channels, alpha(1)C and alpha(1)D, and one isoform of T channel, alpha(1)H. A second T channel isoform (alpha(1)G) was also observed under particular culture conditions. Quantification of the specific messenger RNA by real time RT-PCR allowed us to show a 40% increase of the alpha1H messenger levels upon aldosterone treatment (alpha(1)G was insensitive), a response that was also completely prevented by spironolactone. Because T-type, but not L-type channel activity is linked to steroidogenesis, this modulation represents a positive, intracrine feed back mechanism exerted by aldosterone on its own production. Aldosterone has been also implicated in the pathogenesis and progression of ventricular hypertrophy and heart failure independently of its action on arterial blood pressure. We have observed that, in rat neonatal cardiomyocytes, aldosterone increases (by two-fold) L-type calcium current amplitude in ventricular but not in atrial cells. No significant effect of aldosterone could be detected on T-type currents, that were much smaller than L-type currents in these cells. However, aldosterone exerted opposite effects on T channel isoform expression, increasing alpha(1)H and decreasing alpha(1)G. Although the functional role of T channels is still poorly defined in ventricular cardiomyocytes, an overexpression of alpha(1)H could be partially responsible for the arrhythmias linked to hyperaldosteronism.Finally, T channels also appear to be involved in the neuroendocrine differentiation of prostate epithelial cells, a poor prognosis in prostate cancer. We have shown that the only calcium channel expressed in the prostatic LNCaP cells is the alpha(1)H isoform and that induction of cell differentiation with cAMP leads to a concomitant increase in both T-type current and alpha(1)H mRNA. In spite of the presence of MR in these cells, aldosterone only modestly increased alpha(1)H mRNA levels. A functional role for these channels was suggested by the observation that low nickel concentrations prevent neuritic process outgrowth. In conclusion, it appears that T-type calcium channel expression vary in different patho-physiological conditions and that aldosterone, in several cell types, is able to modulate this expression.
Rossier
Michel F
MF
Division of Endocrinology & Diabetology, University Hospital of Geneva, 24 rue Micheli-du-Crest, CH-1211 Geneva 14, Switzerland . michel.rossier@medecine.unige.ch
Lesouhaitier
Olivier
O
Perrier
Emeline
E
Bockhorn
Liliane
L
Chiappe
Alberto
A
Lalevée
Nathalie
N
D000310:Adrenal Gland Neoplasms; D000450:Aldosterone / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D020747:Calcium Channels, T-Type / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D020013:Calcium Signaling; D002478:Cells, Cultured; D006321:Heart / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D008958:Models, Molecular; D032342:Muscle Cells / Q000502:physiology; D011471:Prostatic Neoplasms; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D051381:Rats; D014407:Tumor Cells, Cultured
D020747:Calcium Channels, T-Type; D021122:Protein Subunits; D000450:Aldosterone