[Induction of primary immune response by rat dendritic cells].
In this work we generated dendritic cells (DC) from rat bone marrow cultures stimulated with GM-CSF and IL-4. After 10 days of culture, we obtained numerous mature DC showing morphological characteristics of DC and high levels of MHC class II molecules. Also, we isolated DC from rat spleen on the bases of their differential adherence and low-density properties. The purity of these cells was > 90% according to morphology and MHC class II expression. To evaluate the capacity of bone marrow DC, immature spleen DC or spleen DC cultured 24h with GM-CSF (mature spleen DC), to elicit an immune response to ovalbumin (OVA), DC were loaded with this antigen and transferred to normal rats. Both bone marrow and spleen DC induced delayed type hypersensitivity responses (DTH). However, mature DC from spleen induced a stronger immune response against OVA with the highest DTH values (p < 0.05). These differences in the induction of the immune response correlated with higher expression of MHC class II molecules on mature DC.
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Inmunología, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba (5000), Rep. Argentina.
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Riera
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Iribarren
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D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology* / Q000648:ultrastructure; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D015847:Interleukin-4; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology*
D000949:Histocompatibility Antigens Class II; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin