title: Release of protein-bound residues of thiabendazole from liver.
abstract: Tissue-bound residues of thiabendazole (TBZ), a veterinary anthelmintic and postharvest fungicide, are formed when this compound is incubated with rabbit hepatocytes or administered to mice or pigs. Several pretreatment steps were investigated for removing free TBZ and metabolites prior to the release of bound residues, and three procedures were evaluated for the release of bound residues from solvent-extracted rabbit hepatocytes: incubation under acidic conditions, enzymatic action using cystathionine beta-lyase, and Raney nickel desulfurization. Immunoaffinity chromatography utilizes monoclonal antibodies capable of binding TBZ or its 5-hydroxy metabolite enabled isolation of crossreactive residue fractions. Residues released from incurred pig liver and isolated by immunoaffinity included TBZ, as determined by HPLC with photodiode array detection. The methodology described should facilitate food safety assessments of TBZ.
authors.affiliation: Food Safety Department, The National Food Centre, Teagasc, Dunsinea, Castleknock, Dublin 15, Ireland.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Horne
authors.forename: Tiernan
authors.initials: T
authors.lastname: Coyle
authors.forename: Michael
authors.initials: M
authors.lastname: O'Keeffe
authors.forename: Michel
authors.initials: M
authors.lastname: Alvinerie
authors.forename: Pierre
authors.initials: P
authors.lastname: Galtier
authors.forename: David L
authors.initials: DL
authors.lastname: Brandon
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000969:Antinematodal Agents / Q000378:metabolism*; D002846:Chromatography, Affinity; D022781:Hepatocytes / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism; D051379:Mice; D009532:Nickel / Q000737:chemistry; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D013552:Swine; D013827:Thiabendazole / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000969:Antinematodal Agents; D011506:Proteins; D009532:Nickel; D008190:Lyases; C019965:cystathionine beta-lyase; D013827:Thiabendazole