title: [Characterization of anti-DNA antibody on IgG3-kappa type M-protein in a patient with rheumatoid arthritis].
abstract: A 66 year-old male patient with rheumatoid arthritis, was admitted due to bronchial pneumonia. Anti-nuclear antibody and anti-DNA antibody were both detected in the patient's serum and many LE-like cells were found in the pleural effusion. In addition, M-proteins(IgG-kappa type, IgM-kappa type) were detected in both serum and pleural effusion by immunofixation electrophoresis. Purified IgG fraction by gel filtration(Superdex 200HR) showed high titer of anti-nuclear antibody(x160) and anti-DNA(39.2 AU/ml) antibody. Furthermore, anti-DNA antibody was detected in the fraction III(fraction of M-protein) by ion-exchange chromatography. In conclusion, these results indicate that M-protein(IgG3-kappa type) contains anti-DNA antibody and it is suggests that M-protein induces the production of LE like cell in this case.
authors.affiliation: Department of Clinical Pathology, Aomori Prefectural Central Hospital, Aomori 030-8553.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Sato
authors.forename: Norio
authors.initials: N
authors.lastname: Terasawa
authors.forename: Mitsuomi
authors.initials: M
authors.lastname: Kaimori
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Takemori
authors.forename: Teruko
authors.initials: T
authors.lastname: Otake
authors.forename: Ikunosuke
authors.initials: I
authors.lastname: Sakurabayashi
mesh_terms: D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D004247:DNA / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology*; D008297:Male; D009194:Myeloma Proteins / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; C085016:multiple myeloma M-proteins; D004247:DNA