[Mutation of GCK gene of Chinese patients with late-onset type 2 diabetes].
OBJECTIVE
To determine the mutation of the Glucokinase (GCK) gene in Chinese patients with Type 2 diabetes.


METHODS
Forty-seven families with Type 2 diabetes were recruited. GCK gene was analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct sequencing.


RESULTS
Sequence analysis of two abnormal DNA fragments demonstrated a replacement of C-->T at the 38th base in intron 6 and a replacement of C-->T at the 8th base in intron 9, forming two polymorphic sites.


CONCLUSIONS
IVS6--38C-->T and IVS9--8C-->T were found and mutation of GCK gene may not be a major genetic factor in Chinese patients with Type 2 diabetes.
Liu
Zheng
Z
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Deng
Hao
H
Tang
Wei-Li
WL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D005941:Glucokinase