Immunogenicity of polymerizable synthetic peptides derived from a vaccine candidate against schistosomiasis: the asparaginyl endopeptidase (Sm32).
The asparaginyl endopeptidase (Sm32) is expressed in the gastrodermal cells of the schistosome gut and in the head glands of the cercariae. Possibly, Sm32 hydrolyzes pro-proteins involved in the degradation of host hemoglobin [Parasitol. Today 12 (1996) 125]. Preliminary evidences using an Sj32/Sm32 murine vaccine have shown a profound effect on oviposition and worm burden [Chin. J. Schist. Control. 7 (1995) 72; Bull. Human Med. Univ. 24 (1999) 225; Vaccine 20 (2002) 439]. The importance of Sm32 as a novel vaccine candidate is based on the possibility of preventing the maturation of other cathepsins and/or preventing schistosome skin invasion. We studied the immunogenicity of polymerizable peptides derived from Sm32 to select potential protective epitopes. Sm32 prediction of T and B epitopes and homology studies with human legumain were performed. Among the variety of factors that influence the antibody response, we specifically examined the effect of: (i) genetic background of mouse strain, inbred (C57BL/6) versus outbred (Swiss) mice; and (ii) vaccination with a single peptide versus pool of peptides. Swiss mice raised antibodies to three different regions of the Sm32, as tested by the Multiple Antigen Blot Assay (MABA): 182-215 (peptides IMT-70 and 72), 244-273 (IMT-64) and 336-355 (IMT-66). None of these regions were immunogenic for C57BL/6. On the contrary, other peptides, IMT-4 (21-40), IMT-12 (101-120) and IMT-26 (292-313) were highly immunogenic for this inbred strain. Only Swiss mice immunized with a single peptide (IMT-64 and 72) or with three different pools of IMT-peptides (Pool A-II: 14, 16, 18, 70, 72, 89; pool A-III: 22, 64, 24, 26, 28 and pool A-V: 64, 66, 28, 70, 72) recognized the original protein in a crude extract of the worm antigen by Western blot. Peptides IMT-64, 14 and 26 were responsible for this recognition. In general, the vaccination with pool of peptides was more immunogenic for both mouse strains. Predicted B cell epitopes, with hydrophilicity scores over +10 (IMT-12, 64, 26) were always immunogenic after either single or combined peptide vaccination. Sm32 sequences 41-80 (IMT-6 and 8), 141-160 (IMT-16) and 182-215 (IMT-70 and 72) were nearly identical to the corresponding human legumain regions and should be excluded from the human vaccine. We can conclude that the regions of Sm32 that were recognized by antibodies of mice immunized with polymerizable peptides depended on the mice strain and on the hydrophilicity score of the peptides.
Chacón
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N
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela, Apartado Postal 47623, Zona Postal 1041-A, Los Chaguaramos, Caracas, Venezuela.
Losada
S
S
Bermúdez
H
H
Cesari
I M
IM
Hoebeke
J
J
Noya
Oscar
O
D000595:Amino Acid Sequence; D000818:Animals; D003429:Cross Reactions / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000201:enzymology / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology* / Q000469:parasitology; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology; D022223:Vaccines, Subunit / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology*; D000099240:Asparaginyl Endopeptidase
D003546:Cysteine Endopeptidases; D018985:Epitopes, B-Lymphocyte; D022223:Vaccines, Subunit; D000099240:Asparaginyl Endopeptidase