[Ziehl-Neelsen-Victoria blue-iodine green compound staining method showing leprotic bacillus].
OBJECTIVE
To compare compound staining methods showing leprotic bacillus, elastic fibre and cell nucleus etc. in skin biopsy tissue.


METHODS
A compound staining dye composed of Ziehl-Neelsen Phenol Fuchsin, Victoria blue, iodine green and Martius yellow were used in paraffin-embedding skin biopsy tissue.


RESULTS
Leprotic bacilli showed red; cell nuclei, light green; cell cytoplasm, colorless; elastic fibres around tuberculoid nodules, blue; and essential substances, light yellow in skin biopsy tissue.


CONCLUSIONS
Because the color was single in Ziehl-Neelsen staining method, its contrast was poor. The ZN-VB-IG compound staining method may show leprotic bacilli, elastic fibres and cell nuclei etc. distinctly. Therefore, this is a reliable and nice color contrast compound staining method.
Gong
Zhijin
Z
Department of Pathology, First Affiliated Hospital, Second Military Medical University, Shanghai 200433, China.
Zhu
Minghua
M
Zheng
Jianming
J
Zhan
Rongzhou
R
Lin
Wanhe
W
D006801:Humans; D009166:Mycobacterium leprae / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*