title: [Determination of ciprofloxacin in urine using terbium ion as fluorescent probe].
abstract: Factors effecting the fluorescence characteristics of the reaction CPFX-Tb were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Tb. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 x 10(-8)-1.0 x 10(-6) mol.L-1. The regression equation is F = 1.57 + 4.87 x 10(8) c, r = 0.9999 with the detection limit of 5.0 x 10(-9) mol.L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe.
authors.affiliation: Department of Pharmacy, Shandong Medical University, Jinan 250012, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: L H
authors.initials: LH
authors.lastname: Sang
authors.forename: Y L
authors.initials: YL
authors.lastname: Wu
authors.forename: X M
authors.initials: XM
authors.lastname: Cheng
authors.forename: Z D
authors.initials: ZD
authors.lastname: Su
authors.forename: A H
authors.initials: AH
authors.lastname: Yu
mesh_terms: D000890:Anti-Infective Agents / Q000652:urine*; D002614:Chelating Agents; D002939:Ciprofloxacin / Q000652:urine*; D005456:Fluorescent Dyes; D006801:Humans; D013050:Spectrometry, Fluorescence; D013725:Terbium
chemical_list: D000890:Anti-Infective Agents; D002614:Chelating Agents; D005456:Fluorescent Dyes; D013725:Terbium; D002939:Ciprofloxacin