Efficacy of vitamin E, phosphatidyl choline and pyruvate on Abeta neurotoxicity in culture.
Oxidative stress is a pivotal factor in neuronal degeneration including that induced by exposure to amyloid-beta (Abeta). Treatment with antioxidants such as vitamin E can alleviate Abeta neurotoxicity. However, vitamin E was only marginally effective in clinical trials in Alzheimer's disease. Recent studies indicate that treatment with vitamin E (as a-tocopherol), sodium pyruvate and phosphatidyl choline (PC) is more effective than vitamin E alone against neuronal oxidative stress. We demonstrate herein that treatment of cultured murine cortical neurons with these 3 agents is also more effective than vitamin E alone against Abeta neurotoxicity as assayed by generation of reactive oxygen species and increased levels of phospho-isoforms of the microtubule-associated protein tau. These data underscore the potential efficacy of a combinatorial neuroprotective formulation against Abeta neurotoxicity.
Shea
T B
TB
Center for Cellular Neurobiology and Neurodegeneration Research, Department of Biological Sciences, University of Massachusetts-Lowell, Lowell, MA 01854, USA. Thomas_Shea@uml.edu
Ekinci
F J
FJ
Ortiz
D
D
Wilson
T O
TO
Nicolosi
R J
RJ
D016229:Amyloid beta-Peptides / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004357:Drug Synergism; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D030781:Organisms, Genetically Modified; D018384:Oxidative Stress / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011773:Pyruvates / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D018696:Neuroprotective Agents; D010713:Phosphatidylcholines; D011773:Pyruvates; D014810:Vitamin E