[RAPD-polymerase chain reaction analysis of the genetic structure of tumor hepatocyte population MH-22A].
The viability and genetic variability of mouse hepatoma MH-22a were studied by RAPD-PCR method using 3 primers, and the genetic structure of the tumor hepatocyte population based on the number of clonal fingerprint rearrangements was identified: 56% of hepatocytes made up clones with 0-3 rearrangements (the stem line of the population); 32%--the variable part with 4-7 rearrangements per clone and 12%--the aberrant part with 8-12 rearrangements per clone. The stem line appeared the most viable featuring zero or least genetic variability while the clones characterized by the greatest genetic variability were least viable.
Aleksandrova
S A
SA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Shvemberger
I N
IN
D000818:Animals; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D017931:DNA Primers; D005819:Genetic Markers; D014644:Genetic Variation; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D005819:Genetic Markers