title: Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.
abstract: The N-terminal type II cohesin from the cellulosomal ScaB subunit of Acetivibrio cellulolyticus was crystallized in two different crystal systems: orthorhombic (space group P2(1)2(1)2(1)), with unit-cell parameters a = 37.455, b = 55.780, c = 87.912 A, and trigonal (space group P3(1)21), with unit-cell parameters a = 55.088, b = 55.088, c = 112.553 A. The two crystals diffracted to 1.2 and 1.9 A, respectively. A selenomethionine derivative was also crystallized and exhibited trigonal symmetry (space group P3(1)21), with unit-cell parameters a = 55.281, b = 55.281, c = 112.449 A and a diffraction limit of 1.97 A. Initial phasing of the trigonal crystals was successfully performed by the SIRAS method using Cu Kalpha radiation with the selenomethionine derivative as a heavy-atom derivative. The structure of the orthorhombic crystal form was solved by molecular replacement using the coordinates of the trigonal form.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 68790, Israel.
authors.forename: Ilit
authors.initials: I
authors.lastname: Noach
authors.forename: Raphael
authors.initials: R
authors.lastname: Lamed
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.forename: Sonia
authors.initials: S
authors.lastname: Rosenheck
authors.forename: Linda J W
authors.initials: LJ
authors.lastname: Shimon
authors.forename: Edward A
authors.initials: EA
authors.lastname: Bayer
authors.forename: Felix
authors.initials: F
authors.lastname: Frolow
mesh_terms: D001421:Bacteria, Anaerobic / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry; D018797:Cell Cycle Proteins; D044603:Cellulosomes / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D005656:Fungal Proteins; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D012645:Selenomethionine; D000097722:Cohesins
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012645:Selenomethionine; D000097722:Cohesins