title: Tools for genetic engineering in the amino acid-producing bacterium Corynebacterium glutamicum.
abstract: During the last decades, the gram-positive soil bacterium Corynebacterium glutamicum has been shown to be a very versatile microorganism for the large-scale fermentative production of L-amino acids. Up to now, a vast amount of techniques and tools for genetic engineering and amplification of relevant structural genes have been developed. The objectives of this study are to summarize the published literature on tools for genetic engineering in C. glutamicum and to focus on new sophisticated and highly efficient methods in the fields of DNA transfer techniques, cloning vectors, integrative genetic tools, and antibiotic-free self-cloning. This repertoire of C. glutamicum methodology provides an experimental basis for efficient genetic analyses of the recently completed genome sequence.
authors.affiliation: Lehrstuhl für Gentechnologie/Mikrobiologie, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Kirchner
authors.forename: Andreas
authors.initials: A
authors.lastname: Tauch
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins