title: Distinct transcriptional activation functions of STAT1alpha and STAT1beta on DNA and chromatin templates.
abstract: Interferon-induced transcription depends upon tyrosine phosphorylation, subsequent dimerization, and binding to DNA of STAT1. Other factors, including but not necessarily limited to CBP/p300, then bind within the C-terminal 38 amino acid transactivation domain (TAD) to activate transcription. We show that both tyrosine-phosphorylated STAT1alpha (full-length wild-type protein) and STAT1beta (lacking the TAD) stimulate in vitro transcription on a naked DNA template. Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta-dependent transcription is stimulated by the TRAP/Mediator co-activator complex. Thus STAT1, through some site other than the C-terminal TAD, can interact with TRAP/Mediator or some intermediate protein. Although both STAT1alpha and STAT1beta bind to known STAT sites within in vitro assembled chromatin templates, only STAT1alpha, and not STAT1beta, in cooperation with p300 and acetyl-CoA, stimulated in vitro transcription from chromatin. After interferon-gamma treatment, cells recruit STAT1alpha or -beta to the chromosomal interferon-1 gene, but only STAT1alpha-containing cells recruit p300 and stimulate transcription. We conclude that chromatin remodeling by p300 in vivo makes TRAP/Mediator effective in stimulating transcription.
authors.affiliation: Laboratories of Molecular Cell Biology and Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Zakharova
authors.forename: Elena S
authors.initials: ES
authors.lastname: Lymar
authors.forename: Edward
authors.initials: E
authors.lastname: Yang
authors.forename: Sohail
authors.initials: S
authors.lastname: Malik
authors.forename: J Jillian
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Robert G
authors.initials: RG
authors.lastname: Roeder
authors.forename: James E
authors.initials: JE
authors.lastname: Darnell
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004330:Drosophila; D006367:HeLa Cells; D006801:Humans; D007313:Insecta; D050832:Interferon-Stimulated Gene Factor 3; D007372:Interferons / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D050832:Interferon-Stimulated Gene Factor 3; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; C079868:gamma interferon activation factor; D004247:DNA; D007372:Interferons; D003850:Deoxyribonuclease I