Isolation and culture of umbilical vein mesenchymal stem cells.
Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when properly stimulated, gave origin to adipocytes and osteocytes in culture. Immunophenotypically, this cell population was found to be positive for the CD29, CD13, CD44, CD49e, CD54, CD90 and HLA-class 1 markers and negative for CD45, CD14, glycophorin A, HLA-DR, CD51/61, CD106, and CD49d. The characteristics described are the same as those presented by bone marrow mesenchymal stem cells. Taken together, these findings indicate that the umbilical cord obtained from term deliveries is an important source of mesenchymal stem cells that could be used in cell therapy protocols.
Covas
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DT
Centro de Terapia Celular (CEPID-FAPESP), Fundação Hemocentro de Ribeirão Preto, Centro Regional de Hemoterapia, Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil. dimas@fmrp.usp.br
Siufi
J L C
JL
Silva
A R L
AR
Orellana
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MD
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D016130:Immunophenotyping; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014471:Umbilical Veins / Q000166:cytology*