Determination of glutamine and serine in rat cerebrospinal fluid using capillary electrochromatography with a modified photopolymerized sol-gel monolithic column.
Capillary electrochromatographic separations of amino acid mixtures were studied using two modified porous photopolymerized sol-gel monolithic columns. One was modified with dimethyloctadecylchlorosilane (DMOS), and the other was modified with DMOS, followed by chlorotrimethylsilane to end-cap residual silanol groups. Prior to separation, amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole using as a mobile phase 50 mM phosphate (pH 2.5), water, and acetonitrile in the ratio of 1:1:8. Five derivatized amino acids (Asn, Phe, Ala, Ile, and Leu) were separated within 7 min. Theoretical plate numbers varied between 58700 and 105000/m. This separation method with the end-capped monolithic column was applied to rat cerebrospinal fluid. The dominant amino acid found was Gln at a concentration of 420 microM along with small quantities of Ser (54 microM).
Kato
Masaru
M
Department of Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada Shizuoka, Shizuoka, 422-8526, Japan. daikato@u-shizuoka-ken.ac.jp
Jin
HongMei
H
Sakai-Kato
Kumiko
K
Toyo'oka
Toshimasa
T
Dulay
Maria T
MT
Zare
Richard N
RN
D000818:Animals; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005973:Glutamine / Q000134:cerebrospinal fluid*; D010777:Photochemistry; D051381:Rats; D012694:Serine / Q000134:cerebrospinal fluid*
D005973:Glutamine; D012694:Serine