title: Expression of class I MHC molecule, HSP70 and TAP in human hepatocellular carcinoma.
abstract: OBJECTIVE
  To demonstrate whether class I MHC molecule, transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine against hepatocellular carcinoma (HCC).
  
  
  METHODS
  We studied 30 HCC specimens by labeled streptavidin biotin (LSAB) method of immunohistochemistry.
  
  
  RESULTS
  The results showed that the majority of HCC cells investigated naturally expressed class I MHC and TAP, which were different from other tumor cells. Furthermore, we found that HSP70 expressed not only in cellular cytoplasm, but also on the cell surface in HCC.
  
  
  CONCLUSIONS
  Our findings indicate that our understanding about immune escape mechanisms employed by HCC cells may be further improved. It is important to design and construct CTL vaccine against HCC.
authors.affiliation: Immunology Department, West China Medical Center of Sichuan University, Chengdu 610044, Sichuan Province, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Deng
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Cai
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Wei
mesh_terms: D018528:ATP-Binding Cassette Transporters; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D018840:HSP70 Heat-Shock Proteins; D015395:Histocompatibility Antigens Class I; C477327:transporter associated with antigen processing (TAP)