title: The influence of the apolipoprotein E gene promoter (-219G/ T) polymorphism on postprandial lipoprotein metabolism in young normolipemic males.
abstract: The apolipoprotein E (apoE) gene promoter (-219G/T) polymorphism has been associated with increased risk of myocardial infarction, premature coronary heart disease, and decreased plasma apoE concentrations. We examined whether the -219G/T polymorphism could modify the postprandial response of triacylglycerol-rich lipoproteins (TRLs). Fifty-one healthy apoE 3/3 male volunteers (14GG, 29GT, and 8TT) were given a vitamin A fat-loading test consisting of 1 g of fat/kg body weight and 60,000 IU of vitamin A per m2 of body surface area. Blood samples were taken at time 0 and every hour until the sixth hour, and every 2 hours and 30 minutes until the eleventh hour. Cholesterol, triacylglycerols (TGs), and apoE were determined in plasma; and cholesterol, TG, apoB-100, apoB-48, and retinyl palmitate (RP) were analyzed in lipoprotein fractions. Postprandial lipemia data revealed that subjects with the -219TT genotype had a higher postprandial response of large TRL-cholesterol (P < 0.03), large TRL-triacylglycerols (P < 0.001), large TRL-RP (P < 0.004), and small TRL-apoB-48 (P < 0.03) than carriers of the -219G allele. Moreover, the -219TT subjects had the lowest postprandial levels of serum apoE (P < 0.05). In conclusion, the -219G/T polymorphism may influence TRL metabolism during the postprandial period, thus prolonging postprandial lipemia in subjects with the TT genotype.
authors.affiliation: Lipids and Atherosclerosis Research Unit, Hospital Reina Sofía University, Córdoba, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Moreno
authors.forename: José
authors.initials: J
authors.lastname: López-Miranda
authors.forename: Carmen
authors.initials: C
authors.lastname: Marín
authors.forename: Purificación
authors.initials: P
authors.lastname: Gómez
authors.forename: Pablo
authors.initials: P
authors.lastname: Pérez-Martínez
authors.forename: Francisco
authors.initials: F
authors.lastname: Fuentes
authors.forename: Rafael Angel
authors.initials: RA
authors.lastname: Fernández de la Puebla
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Paniagua
authors.forename: José María
authors.initials: JM
authors.lastname: Ordovas
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Jiménez
mesh_terms: D000293:Adolescent; D000328:Adult; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D019518:Postprandial Period; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D008074:Lipoproteins