Seroprevalence of rickettsial infection in commensal rodents and shrews trapped in the Bangkok Metropolitan Area.
Murine typhus and scrub typhus are important human rickettsial diseases in Thailand. Small mammals, including many species of rodents and shrews, serve as the reservoir host of rickettsial diseases. Rickettsia typhi can be transmitted to humans by fleas causing murine typhus, while infection with Orientia tsutsugamushi causing scrub typhus in humans is transmitted by chiggers. The prevalence of rickettsial infection depends on the geographic area. The seroprevalence of antibody to R. typhi and O. tsutsugamushi was studied in commensal rodents and shrews trapped in markets in the Bangkok Metropolitan Area (BMA). R. typhi and O. tsutsugamushi antigen prepared in the yolk sac of embryonated eggs were used to determine the specific antibody in trapped animals' sera by using fluorescein isothiocyanate (FITC)-anti rat immunoglobulins as a second antibody. Antibody to R. typhi was found in 25 (5%) of 500 sera tested and no antibody to O. tsutsugamushi was detected. R. typhi antibody titer ranged from 40-1280 and was found in Rattus norvegicus (4.2%), Rattus rattus (0.4%), Rattus exulans (0.2%), and Mus musculus (0.2%) trapped in 8 of 47 markets in the BMA. R. typhi antibody was commonly found in R. norvegicus. The authors concluded that murine typhus is an important rickettsial disease and R. norvegicus is an important reservoir species of rodents found in markets of the BMA.
Siritantikorn
Sontana
S
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Sangkasuwan
Vichai
V
Eamsila
Chirapa
C
Singchai
Chantra
C
Kantakamalakul
Wannee
W
Puthavathana
Pilaipan
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D009115:Muridae; D051381:Rats; D012282:Rickettsia Infections / Q000453:epidemiology* / Q000662:veterinary; D012376:Rodent Diseases / Q000453:epidemiology*; D012612:Scrub Typhus / Q000453:epidemiology / Q000662:veterinary; D016036:Seroepidemiologic Studies; D013785:Thailand / Q000453:epidemiology; D014418:Tupaiidae; D014437:Typhus, Endemic Flea-Borne / Q000453:epidemiology / Q000662:veterinary; D014504:Urban Health
D000907:Antibodies, Bacterial