title: In vitro selection for enzymatic activity: a model study using adenylate cyclase.
abstract: An in vitro enzyme selection that can, in principle, be generalised to most chemical reactions, is described. It makes use of filamentous phage display and of a tailor-made antibody fragment directed against the reaction product. The conversion of ATP into 3',5'-cyclic AMP catalysed by Bordetella pertussis adenylate cyclase is taken as an example.
authors.affiliation: Unité de Chimie Organique, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris 15, France.
authors.forename: Heike
authors.initials: H
authors.lastname: Strobel
authors.forename: Daniel
authors.initials: D
authors.lastname: Ladant
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Jestin
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001886:Bordetella pertussis / Q000201:enzymology; D000242:Cyclic AMP / Q000737:chemistry / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000378:metabolism; D015394:Molecular Structure; D019151:Peptide Library
chemical_list: D001426:Bacterial Proteins; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D000242:Cyclic AMP; D000262:Adenylyl Cyclases