title: [Synthesis and characterization of a time-resolved fluorescence analysis chelate reagent-BCPDA].
abstract: In this paper, we report on a solid phase time-resolved fluorescence immunoassay chelate reagent-4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA), which is suitable as a fluorescent labeling agent. The five step synthesis product of BCPDA was presented for improving the purity of the product based on the three step synthesis product. The approach involves chlorization, hydrolyzing the ester, preparing disodium, carboxylate to diacid, sulfonation. The yield of five step product is 99%, 45%, 94%, 95%, 80% respectively. The structure and purity of product was characterized by the melting point, IR, 1H NMR, UV spectrum, element analysis, and proved to be consistent with the structure predictal.
authors.affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, National Analytical Research Center of Electrochemistry and Spectroscopy, Key Laboratory of Rare Earth Chemistry and Physics, Changchun 130022, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Pan
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Ding
authors.forename: Wen-wei
authors.initials: WW
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D010618:Phenanthrolines / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D010618:Phenanthrolines; C054206:4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid