title: The decline in B lymphopoiesis in aged mice reflects loss of very early B-lineage precursors.
abstract: The primary age-related loss in B cell progenitors is thought to be at the pro- to pre-B cell transition. However, we show that the frequencies and absolute numbers of all progenitor populations for the B cell lineage, including B-lineage-committed pro-B cells and multipotent B-lymphoid progenitors, decline in aged C57BL/6 mice. Moreover, when derived from aged mice, lymphoid progenitors within every population examined exhibited suboptimal IL-7 responsiveness, demonstrating that age-associated suboptimal IL-7R signaling is a general property of all early B-lineage precursors. Collectively, these data indicate that aging results in a previously unappreciated decline in the earliest stages of B cell development.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
authors.forename: Juli P
authors.initials: JP
authors.lastname: Miller
authors.forename: David
authors.initials: D
authors.lastname: Allman
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000145:classification* / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000145:classification / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019070:Cell Lineage / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation / Q000276:immunology*; D016193:G1 Phase / Q000276:immunology; D016195:G2 Phase / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000145:classification* / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018960:Hyaluronan Receptors; D016130:Immunophenotyping; D015851:Interleukin-7 / Q000502:physiology; D018655:Lymphocyte Count; D038041:Lymphopoiesis / Q000276:immunology*; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins; D011951:Receptors, Complement / Q000096:biosynthesis
chemical_list: C490193:C1qbp protein, mouse; D018960:Hyaluronan Receptors; D015851:Interleukin-7; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor