Use of adult marrow mesenchymal stem cells for regeneration of cardiomyocytes.
Bone marrow cells have been suggested to have multilineage plasticity including formation of cardiac tissue. We have established a cardiomyogenic (CMG) cell line from mouse bone marrow stromal cells that can be induced to differentiate into cardiomyocytes in vitro by 5-azacytidine treatment. A number of lines of evidence confirm the cardiomyocyte characteristics of CMG cells.
Fukuda
K
K
Institute for Advanced Cardiac Therapeutics, Keio University School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo, Japan.
D000200:Action Potentials; D000328:Adult; D001374:Azacitidine / Q000494:pharmacology; D001854:Bone Marrow Cells; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D011506:Proteins / Q000032:analysis; D012038:Regeneration; D022142:Secretory Vesicles; D017154:Stromal Cells / Q000166:cytology
D011506:Proteins; D001374:Azacitidine