Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and northeastern Colombia.
Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (F ST: 0.035; Nm: 6.8) but lower between these two and the northeastern population (F ST: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (phi ST 0.1131, P < 0.001). The variation among individuals within populations (phi ST 0.8869, P < 0.001)was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific.
Posso
Carmen Elisa
CE
Departamento de Biología, Facultad de Ciencias, Universidad del Valle, apartado Aéreo 25360, Cali, Colombia. ceposso@univalle.edu.co
González
Ranulfo
R
Cárdenas
Heiber
H
Gallego
Gerardo
G
Duque
Myriam Cristina
MC
Suarez
Marco Fidel
MF
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D003105:Colombia; D017344:Genes, Insect / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D006579:Heterozygote; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers