Heat shock transcription factor HSF1 is required for survival of sensory hair cells against acoustic overexposure.
To analyze the role of heat shock response in the cochleae, we induced major heat shock proteins, Hsp70, Hsp90, and Hsp27 by perfusion of hot saline into the middle ear cavity (called 'local heat shock') in guinea pigs. Hsps were induced in almost all of the cochlear cells including the sensory hair cells in the organ of Corti. We showed that loss of both the sensory hair cells and the auditory function induced by acoustic overexposure was inhibited by pretreatment of the inner ear with local heat shock. To examine the role of heat shock transcription factor 1(HSF), which activates heat shock genes in response to heat shock, in the protection of sensory hair cells, we analyzed acoustic injury in HSF1-null mice. We found that the loss of sensory hair cells was more significant in HSF1-null mice compared with that of wild-type mice when mice were subjected to acoustic overexposure. These results indicate that HSF1 is required for survival of the sensory hair cells against acoustic overexposure.
Sugahara
Kazuma
K
Department of Biochemistry and Molecular Biology, Yamaguchi University School of Medicine, Minami-Kogushi 1-1-1, Ube, Yamaguchi 755-8505, Japan.
Inouye
Sachiye
S
Izu
Hanae
H
Katoh
Yumiko
Y
Katsuki
Kensaku
K
Takemoto
Tsuyoshi
T
Shimogori
Hiroaki
H
Yamashita
Hiroshi
H
Nakai
Akira
A
D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology* / Q000503:physiopathology; D006317:Hearing Loss, Noise-Induced / Q000517:prevention & control; D000076249:Heat Shock Transcription Factors; D006358:Hot Temperature / Q000009:adverse effects*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009622:Noise / Q000009:adverse effects*; D014157:Transcription Factors / Q000502:physiology
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors