Development of an "electroptode" immunosensor: indium tin oxide-coated optical fiber tips conjugated with an electropolymerized thin film with conjugated cholera toxin B subunit.
We demonstrate that it is possible to create surface-conductive fiber optics, upon which may be electropolymerized a biotinylated polypyrrole thin film, which may then be used to affinity coat the fiber with molecular recognition probes. This fiber-optic electroconductive surface modification is done by the deposition of a thin layer of indium tin oxide. Thereafter, biotin-pyrrole monomers are electropolymerized onto the conductive metal oxide surface and then exposed to avidin. Avidin-biotin interactions were used to modify the fiber optics with biotin-conjugated cholera toxin B subunit molecules, for the construction of an immunosensor to detect cholera antitoxin antibodies. The biosensor was tested for sensitivity, nonspecificity, and overall practicality.
Konry
Tania
T
The Institute for Applied Biosciences, Ben Gurion University of the Negev, P.O. Box 653, Beer Sheva, 84105, Israel.
Novoa
Andres
A
Cosnier
Serge
S
Marks
Robert S
RS
D000906:Antibodies / Q000032:analysis; D001360:Avidin / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001710:Biotin / Q000737:chemistry; D002772:Cholera Toxin / Q000737:chemistry* / Q000276:immunology; D005336:Fiber Optic Technology; D007118:Immunoassay; D055100:Optical Fibers; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry*
D000906:Antibodies; D011108:Polymers; D011758:Pyrroles; D017971:Tin Compounds; D001360:Avidin; D001710:Biotin; C109984:indium tin oxide; D002772:Cholera Toxin