Crystallization and preliminary X-ray diffraction studies of a toxic phospholipase A2 from the venom of Vipera ammodytes meridionalis complexed to a synthetic inhibitor.
A toxic phospholipase A(2) (PLA(2)) is isolated from the neurotoxic complex Vipoxin, the major lethal component of the venom of Vipera ammodytes meridionalis. The enzyme is complexed to the synthetic inhibitor elaidoylamide and crystallized. The crystals belong to the space group P2(1)2(1)2(1), with unit cell dimensions a=46.57 A, b=82.68 A, c=119.47 A and beta=90 degrees. Initial diffraction data to 3.3 A resolution are collected.
Georgieva
Dessislava Nikolova
DN
Universitätsklinikum Hamburg-Eppendorf, Zentrum für Experimentelle Medizin, Institut für Biochemie und Molekularbiologie I, c/o DESY, Notkestrasse 85, Geb. 22a, 22603 Hamburg, Germany.
Rypniewski
Wojciech
W
Perbandt
Markus
M
Jain
Mahendra
M
Genov
Nicolay
N
Betzel
Christian
C
D000577:Amides / Q000378:metabolism; D000818:Animals; D018360:Crystallography, X-Ray; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D054467:Phospholipases A2; D014757:Viper Venoms / Q000737:chemistry* / Q000235:genetics; D017819:Viperidae / Q000235:genetics*
D000577:Amides; D014757:Viper Venoms; C033134:vipoxin; D010741:Phospholipases A; D054467:Phospholipases A2