Two STR loci polymorphisms of adenosine deaminase gene in Japanese population.
Polymorphism of two tetranucleotide tandem repeat loci in the adenosine deaminase (ADA) gene was studied in a Japanese population. A TAAA repeat at approximately 1100 nucleotide 5' to the ADA transcription initiation site, and a TTTA repeat within intron 3 of the ADA gene were characterized using the polymerase chain reaction (PCR) and sequence analysis. Among the 235 individuals studied, seventeen genotypes at the ADA-TAAA locus and ten genotypes at the ADA-TTTA locus were identified, yielding discrimination potentials of 0.90 and 0.66, respectively. The observed and expected genotype values are consistent with Hardy-Weinberg equilibrium. The allele frequencies in the Japanese population were compared with the reported values for other populations. At the ADA-TAAA locus, there were significant differences between the Japanese population and an American Caucasian sample. At the ADA-TTTA locus, no significant differences were observed between Japanese and Chinese or between Japanese and Basques, but significant differences were found between our Japanese population and Italians and Mossi.
Tie
Jian
J
Department of Legal Medicine, Nihon University School of Medicine, Tokyo, Japan. jtie@med.nihon-u.ac.jp
Suzuki
Yuka
Y
Tsukamoto
Shojiro
S
Oshida
Shigemi
S