[FTIR study on the normal and cancerous stomach tissues].
Tissues of cancerous and corresponding normal stomach were studied by FTIR technique. The results showed that there are obvious differences between FTIR spectra of them in spectral parameters such as frequency, intensity and band shape etc. The changes involving the phosphate symmetric stretching nu s, PO2- and asymmetric stretching nu as, PO2- modes, the CH3 and CH2 groups stretching (nu s, CH2, nu as, CH3) and bending (delta CH2) modes and the C-O stretching nu C-O mode were discussed. In addition, the changes of structure of hydrogen-bonding of nucleic acid and cell proteins and the packing and the conformational structure of the membrance lipids were analysed further. The average wavenumber of band of nu s, PO2- shifted from 1,080.92 cm-1 to 1,085.93 cm-1 and that of nu as, PO2- shifted from 1,239.64 cm-1 to 1,238.73 cm-1 which indicated that the degree of hydrogen-bonding formed by oxygen atom of the phosphodiester groups of nucleic acids was increased. The average wavenumber of band of delta CH2 of membrance lipids shifted from 1,455.23 cm-1 to 1,457.37 cm-1 that suggested that the conformational structure of the methylene chains of membrance lipids is more disordered than in normal tissues. The shift of band of nu C-O of cell proteins from 1,166.08 cm-1 to 1,166.58 cm-1 indicated that the hydrogen-bond of cell proteins become weaker.
Tong
Y
Y
Department of Biochemistry, Hanshan Normal College, 521041 Chaozhou.
Lin
Y
Y
D000230:Adenocarcinoma / Q000737:chemistry*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D008875:Middle Aged; D009696:Nucleic Acids / Q000032:analysis*; D012815:Signal Processing, Computer-Assisted; D017550:Spectroscopy, Fourier Transform Infrared; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000737:chemistry*
D009696:Nucleic Acids