title: Characterization of NADE, NRIF and SC-1 gene expression during mouse neurogenesis.
abstract: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis factor receptor superfamily. p75NTR signaling events have been implicated in both cell cycle arrest and apoptosis depending on which effector molecules are associated with its intracellular domain after ligand binding. Two such effector proteins, p75NTR-associated cell death executor (NADE) and neurotrophin receptor interacting factor (NRIF) promote p75NTR-mediated apoptosis, whereas Schwann cell factor-1 (SC-1) mediates neurotrophin-dependent withdrawal from the cell cycle. An understanding of the expression profiles of these three interacting proteins and p75NTR during embryogenesis is critical for addressing whether these effector proteins might function outside of p75NTR-mediated signaling events. The distribution of NADE, NRIF and SC-1 mRNAs during murine development suggests that the action of these genes is in fact not limited to regions of p75NTR expression. Specifically, a detailed comparison of the spatial and temporal expression domains of NADE, NRIF and SC-1 during brain development revealed regions of co-expression with p75NTR but also illustrates a distinct and discordant spatial and temporal expression. These results yield novel insights into the unique developmental characteristics of the three p75NTR-interacting proteins, thus revealing their diverse signaling potential during embryonic development.
authors.affiliation: The Center of Regenerative Medicine, The Laboratory of Stem Cell Biology, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074, USA.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Kendall
authors.forename: Michael C
authors.initials: MC
authors.lastname: Ryczko
authors.forename: Mala
authors.initials: M
authors.lastname: Mehan
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Verdi
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004268:DNA-Binding Proteins; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C485842:Ngfrap1 protein, mouse; D011506:Proteins; D012333:RNA, Messenger; C402289:Zfp110 protein, mouse