title: Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle.
abstract: An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ota
authors.forename: Atsushi
authors.initials: A
authors.lastname: Arakaki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D020137:Base Pair Mismatch / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D020099:Coated Materials, Biocompatible; D008565:Membrane Proteins; C405425:MpsA protein, Magnetospirillum sp.; D015345:Oligonucleotide Probes