title: Bacteriophages provide regulatory signals in mitogen-induced murine splenocyte proliferation.
abstract: The aim of this investigation was to reveal the regulatory properties of bacteriophage preparations in a model of mitogen-induced splenocyte proliferation in mice. We showed that sepharose 4B-purified preparations of the Staphylococcus aureus phage A20/R exhibited costimulatory activity in splenocyte proliferation induced by suboptimal (0.25 microg/ml) concentrations of ConA. On the other hand, the purified phage fraction was regulatory with regard to splenocyte proliferation induced by the optimal (2.5 microg/ml) ConA concentration. We also showed that the phage preparation can elicit IL-6 production in splenocyte cultures and enhance ConA-induced production of that cytokine. Furthermore, the phages preferentially induced IL-6 production in adherent splenocytes and increased levels of that cytokine in cultures of peritoneal cells from mice and rats. This phenomenon may explain the costimulatory activity of phages in the model described.
authors.affiliation: Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Zimecki
authors.forename: Beata
authors.initials: B
authors.lastname: Weber-Dabrowska
authors.forename: Marzanna
authors.initials: M
authors.lastname: Łusiak-Szelachowska
authors.forename: Marian
authors.initials: M
authors.lastname: Mulczyk
authors.forename: Janusz
authors.initials: J
authors.lastname: Boratyński
authors.forename: Gryzelda
authors.initials: G
authors.lastname: Poźniak
authors.forename: Danuta
authors.initials: D
authors.lastname: Syper
authors.forename: Andrzej
authors.initials: A
authors.lastname: Górski
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D003124:Colorimetry; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013211:Staphylococcus aureus / Q000254:growth & development
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A