title: Live cell catapulting and recultivation.
abstract: Laser micromanipulation systems are used worldwide in the field of life science research. Most of their applications focus on the isolation of specific cells from different types of tissue and the manipulation of subcellular structures within fixed or living cells. Using the PALM MicroBeam, it is possible to microdissect living cells from a cell culture, to catapult them into collection devices, and to re-cultivate the isolated cells. For this purpose, new protocols and special equipment were developed. It has also been demonstrated that Laser Microdissection and Pressure Catapulting (LMPC) have no influence on the proliferation rate of the cells. Even re-cultivated cell colonies, trypsinized and seeded out again, are still viable after a second LMPC-procedure. This new approach opens a wide field of interesting applications in cell biology, molecular pathology, and pharmacology.
authors.affiliation: P.A.L.M. Microlaser Technologies AG, Bernried, Germany. monika.stich@palm-microlaser.com
authors.forename: Monika
authors.initials: M
authors.lastname: Stich
authors.forename: Stefan
authors.initials: S
authors.lastname: Thalhammer
authors.forename: Renate
authors.initials: R
authors.lastname: Burgemeister
authors.forename: Gabriele
authors.initials: G
authors.lastname: Friedemann
authors.forename: Susanne
authors.initials: S
authors.lastname: Ehnle
authors.forename: Carsten
authors.initials: C
authors.lastname: Lüthy
authors.forename: Karin
authors.initials: K
authors.lastname: Schütze
mesh_terms: D004210:Dissection / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007834:Lasers; D008113:Liver Neoplasms / Q000648:ultrastructure; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure