Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP.
DC-LAMP, a member of the lysosomal-associated membrane protein (LAMP) family, is specifically expressed by human dendritic cells (DC) upon activation and therefore serves as marker of human DC maturation. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. The present study describes the cloning and characterization of mouse DC-LAMP, whose predicted protein sequence is over 50% identical to the human counterpart. The mouse DC-LAMP gene spans over 25 kb and shares syntenic chromosomal localization (16B2-B4 and 3q26) and conserved organization with the human DC-LAMP gene. Analysis of mouse DC-LAMP mRNA and protein revealed the expression in lung peripheral cells, but also its unexpected absence from mouse lymphoid organs and from mouse DC activated either in vitro or in vivo. In conclusion, mouse DC-LAMP is not a marker of mature mouse DC and this observation raises new questions regarding the role of human DC-LAMP in human DC.
Salaun
Bruno
B
Schering Plough, Laboratory for Immunological Research, Dardilly, France.
de Saint-Vis
Blandine
B
Clair-Moninot
Valérie
V
Pin
Jean-Jacques
JJ
Barthélemy-Dubois
Clarisse
C
Kissenpfennig
Adrien
A
Peronne
Catherine
C
Bates
Elizabeth
E
Mattei
Marie-Geneviève
MG
Lebecque
Serge
S
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000378:metabolism; D051907:Lysosomal Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000502:physiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
D015703:Antigens, CD; D051907:Lysosomal Membrane Proteins; D012333:RNA, Messenger