title: [Effects of brain-derived neurotrophic factor on H2O2-induced apoptosis of human arterial smooth muscle cell].
abstract: OBJECTIVE
  To investigate the protective effects of brain-derived neurotrophic factor (BDNF) on hydrogen peroxide (H2O2) induced apoptosis of human arterial smooth muscle cells (SMC).
  
  
  METHODS
  The hBDNF cDNA was transfected into the SMC mediated by Lipofect- AMINE, and ectopic expression in SMC was driven by the regulatory elements of I type collagen gene(COLIA1). The cell proliferation was measured with a colorimetric assay based on the cleavage of the tetrazolium salt MTT. Microscopic analysis of cell apoptosis was performed with fluorescent stain of acridine orange (AO). Apoptosis rate of SMC was tested by flow cytometry (FCM). DNA pattern was examined by agarose gel electrophoresis.
  
  
  RESULTS
  Exposure of growing SMC to 100-800 mumol/L H2O2 for 24 h remarkably suppressed the cell proliferation. After treatment of the cells with 200 mumol/L H2O2 for 24 h, morphological and biochemical changes of classic apoptosis such as condensation of cytoplasm, fragmentation of the nuclear chromatin, DNA "ladder" pattern after electrophoresis were observed. Yet, the above-mentioned apoptosis changes were not observed and the apoptosis rate was reduced obviously in the BDNF-transfected SMC treated with the same concentration of H2O2.
  
  
  CONCLUSIONS
  H2O2 can induce apoptosis of SMC, but BDNF inhibits H2O2-induced SMC apoptosis markedly and has strong anti-apoptotic activity.
authors.affiliation: Institute of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.forename: Ruohan
authors.initials: R
authors.lastname: Wang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D006861:Hydrogen Peroxide; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D014162:Transfection
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D006861:Hydrogen Peroxide