Hyaluronidase in sera of tumour-bearing nude mice.
Cancer cell lines often secrete hyaluronidase, suggesting that this enzyme could be used as a marker of growing tumours. We have measured hyaluronidase in the sera of non-grafted mice and mice grafted with human tumour-derived hyaluronidase-secreting H460M and SA87 cells or non-secreting CB 193 cells. Mouse serum hyaluronidase was measured at pH 3.8 using the enzyme-linked sorbent assay (ELSA) technique by reference to human serum whose activity at pH 3.8 was determined by the Reissig technique. The serum hyaluronidase in non-grafted mice ranged from 310-520 mU l(-1) (mean+/-SD 432+/-70 mU l(-1), median 440 mU l(-1)). Hyaluronidase increased in the sera of tumour-bearing mice grafted with H460M cells or with SA87 cells, but not in the sera of mice grafted with CB 193 cells. Serum hyaluronidase activity in H460M or SA87 tumour-bearing mice correlated with the tumour mass, increased with time, and decreased after tumour removal. Zymography detected two different hyaluronidase forms in the sera of non-grafted mice: type 1 had only one hyaluronidase band and type 2 had five different bands. In both types, enzyme augmentation in tumour-bearing mice correlated with the presence of an additional enzyme band that was not seen in normal sera and that migrated as the cancer cell enzyme did; there was no augmentation of the normal isoform(s). These results show that serum hyaluronidase can be used to follow the development of tumours in mice grafted with hyaluronidase-secreting cells.
Maingonnat
Catherine
C
Department of Molecular Oncology, Centre Henri-Becquerel, Rue d'Amiens, 76000 Rouen, France. catherine.maingonnat@rouen.fnclcc.fr
Courel
Marie-Noëlle
MN
Bertrand
Philippe
P
Vincent
Jean-Claude
JC
Sesboüé
Richard
R
Delpech
Bertrand
B
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D045744:Cell Line, Tumor; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000097:blood* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000097:blood*; D009374:Neoplasms, Experimental / Q000175:diagnosis* / Q000201:enzymology* / Q000473:pathology; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D006821:Hyaluronoglucosaminidase