title: Factors predicting peripheral blood progenitor cell collection from pediatric donors for allogeneic transplantation.
abstract: OBJECTIVE
  Although several studies have reported on the use of children as donors for peripheral blood progenitor cells (PBPC), no specific characteristics have been identified as predictors of PBPC collection in this population. In this study we analyzed predictive factors for PBPC collection in pediatric donors.
  
  
  METHODS
  We retrospectively analyzed factors predicting the yield for a target CD34+ cell dose of > or =4x10(6)/Kg donor or recipient body weight, in 105 aphereses from 76 healthy pediatric donors (36 boys and 40 girls) included in the Spanish National Donor Registry. Mobilization consisted of granulocyte colony-stimulating factor (G-CSF) in single doses of 10 microg/kg per day subcutaneously for 4 or 5 days. Apheresis started after the fourth dose of G-CSF.
  
  
  RESULTS
  Median age and body weight were 10 years (range 1-18) and 42 kg (range 9-89), respectively. The median number of CD34+ cells/kg recipient body weight was 4.22 (range 0.1-32). On multivariate analysis variables that had a significant negative impact on the CD34+ cell yield, considering the recipient's body weight were the total blood volume processed (regression coefficient (RC): 0.41, 95% CI: 0.21-0.81; p=0.01) and day of apheresis other than first (RC: 0.16, 95% CI: 0.07-0.34; p<0.0001). When considering donor's body weight the variables that positively influenced collection were younger age (RC: 6.79, 95% CI: 1.57-29.25; p<0.01) and large volume leukapheresis (RC: 3.33, 95% CI: 1.13-9.77; p<0.02).
  
  
  CONCLUSIONS
  Our data suggest that pediatric donors mobilized by G-CSF may donate sufficient numbers of CD34+ cells for allogeneic transplantation. The variables that influenced the yield were the donor's age, blood volume processed and the first day of the apheresis.
authors.affiliation: Spanish Group for Allogeneic Peripheral Blood Stem Cell Transplantation.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Díaz
authors.forename: Julián
authors.initials: J
authors.lastname: Sevilla
authors.forename: Javier
authors.initials: J
authors.lastname: de la Rubia
authors.forename: Amparo
authors.initials: A
authors.lastname: Verdeguer
authors.forename: Ildefonso
authors.initials: I
authors.lastname: Espigado
authors.forename: Marta G
authors.initials: MG
authors.lastname: Vicent
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Pascual
authors.forename: Concha
authors.initials: C
authors.lastname: Zamora
authors.forename: Rosario
authors.initials: R
authors.lastname: Arrieta
authors.forename: David
authors.initials: D
authors.lastname: Serrano
authors.forename: Consuelo
authors.initials: C
authors.lastname: del Cañizo
authors.forename: Cristina
authors.initials: C
authors.lastname: Arbona
authors.forename: Felipe
authors.initials: F
authors.lastname: de Arriba
authors.forename: Joan
authors.initials: J
authors.lastname: Bargay
authors.forename: Salut
authors.initials: S
authors.lastname: Brunet
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Sanz
mesh_terms: D000293:Adolescent; D000367:Age Factors; D018952:Antigens, CD34 / Q000096:biosynthesis; D001781:Blood Component Removal / Q000379:methods / Q000706:statistics & numerical data; D001782:Blood Donors / Q000706:statistics & numerical data*; D001835:Body Weight; D002452:Cell Count / Q000706:statistics & numerical data; D002648:Child; D002675:Child, Preschool; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods / Q000706:statistics & numerical data; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods / Q000706:statistics & numerical data*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D007223:Infant; D008297:Male; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D013030:Spain; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000706:statistics & numerical data*
chemical_list: D018952:Antigens, CD34