[Resorcinol-spectrophotometric method for the determination of fructose in mustard leaf's amylose].
A method using seliwanoff reaction is proposed for the determination of fructose in the amylose of mustard leaves. Fructose reacts with resorcinol forming a red compound with a maximum absorption at 473 nm. The calibration curve is linear over the range of 0-83 micrograms.mL-1 with a correlation coefficient of 0.9998. The content of fructose in the amylose of mustard leaves is found to be 11.41%. Quantitative analysis used for the fructose of syrup include such as volumetric analysis, then layer chromatography, polarimetry, high pressure liquid chromatography and spectrophotometric analysis. According to different color-developing agents, spectrophotometric analysis may be classified as carbazole method, ammonium molybdate way and resorcinol way etc. reported by foreign papers is hard to operate and colored complex is unstable.
Hasenqimeng
School of Technology, University of Inner Mongolia Nationalities, Tongliao 028043, China.
He
Feng-ga
FG
D000688:Amylose / Q000737:chemistry*; D002138:Calibration; D005632:Fructose / Q000032:analysis* / Q000737:chemistry; D009149:Mustard Plant / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D005632:Fructose; D000688:Amylose