title: Analysis of H2-O influence on antigen presentation by B cells.
abstract: HLA-DM (DM; in mouse H2-DM) promotes the exchange of MHC class II-associated peptides, resulting in the accumulation of stable MHC class II-peptide complexes. In naive (but not germinal center) B cells, a large part of DM is tightly associated with HLA-DO (DO; in mouse H2-O), but the functional consequence of this association for Ag presentation is debated. Here, we have extended previous studies by examining the presentation of multiple epitopes after Ag internalization by fluid phase endocytosis or receptor-mediated uptake by membrane Ig (mIg) receptors. We find that the effects of H2-O are more complex than previously appreciated; thus, while only minor influences on Ag presentation could be detected after fluid phase uptake, many epitopes were substantially affected after mIg-mediated uptake. Unexpectedly, the presentation of different epitopes was found to be enhanced, diminished, or unaffected in the absence of H2-O, depending on the specificity of the mIg used for Ag internalization. Interestingly, epitopes from the same Ag did not necessarily show the same H2-O dependency. This finding suggests that H2-O may control the repertoire of peptides presented by B cells depending on the mIg-Ag interaction. The absence of DO/H2-O from germinal center B cells suggests that this control may be released during B cell maturation.
authors.affiliation: Johnson & Johnson Pharmaceutical Research and Development, 3210 Merryfield Row, San Diego, CA 92121, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Alfonso
authors.forename: G Stuart
authors.initials: GS
authors.lastname: Williams
authors.forename: Jung-Ok
authors.initials: JO
authors.lastname: Han
authors.forename: Johan A
authors.initials: JA
authors.lastname: Westberg
authors.forename: Ola
authors.initials: O
authors.lastname: Winqvist
authors.forename: Lars
authors.initials: L
authors.lastname: Karlsson
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; C416186:H-2O antigen; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D011947:Receptors, Antigen, B-Cell; D014301:Trinitrobenzenes; C038600:trinitrophenyl-ovalbumin; D010047:Ovalbumin; D009113:Muramidase