title: Production and characterization of monoclonal antibodies against midgut of ixodid tick, Haemaphysalis longicornis.
abstract: There are concerted efforts toward development of tick vaccines to replace current chemical control strategies that have serious limitations [Parasitologia 32 (1990) 145; Infectious Disease Clinics of North America (1999) 209-226]. In this study, monoclonal antibodies (mAbs) specific to Haemaphysalis longicornis midgut proteins were produced and characterized. Eight antibody-secreting hybridomas were cloned and the mAbs typed as IgG1, IgG2a and IgG2b. On immunoblots, all mAbs reacted with a midgut protein band of about 76 kDa. All mAbs uniformly immunogold-stained the surface or epithelial layers of H. longicornis midgut and endosomes. Adult ticks (50%) that fed on an ascitic mouse producing the IgGs developed a red coloration and did not oviposit. As such, the 76 kDa protein that reacted with the mAbs could, therefore, be a potential candidate for tick vaccine development.
authors.affiliation: Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Nakajima
authors.forename: Michi
authors.initials: M
authors.lastname: Kodama
authors.forename: Haruko
authors.initials: H
authors.lastname: Yanase
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Iwanaga
authors.forename: Albert
authors.initials: A
authors.lastname: Mulenga
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007132:Immunoglobulin Isotypes; D019476:Insect Proteins / Q000276:immunology*; D026863:Ixodidae / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D013984:Tick Infestations / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D019476:Insect Proteins; D014612:Vaccines