title: Identification and determination of flavonoids in buckwheat (Fagopyrum esculentum Moench, Polygonaceae) by high-performance liquid chromatography with electrospray ionisation mass spectrometry and photodiode array ultraviolet detection.
abstract: An analytical method for flavonoids present in the seed extract of buckwheat (Fagopyrum esculentum Moench, Polygonaceae), using HPLC and a photodiode array detector and interfaced to an electrospray ionisation mass spectrometer, has been developed. Structural information about the flavonols was obtained from the retention time characteristics, the UV-visible spectra and the mass spectra without the need to isolate the individual compounds. The methanol extract of buckwheat contained principally four flavonol glycosides: rutin, quercetin, kaempferol-3-rutinoside and a trace quantity of a flavonol triglycoside.
authors.affiliation: Texas A&M University Kingsville, Citrus Center, 312 N. International Blvd., Weslaco, TX 78596, USA.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Tian
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Bhimanagouda S
authors.initials: BS
authors.lastname: Patil
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts