Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings.
We investigated the ability of synchrotron radiation computed tomography (SR-CT) to demonstrate trabecular microstructure, detail of trabecular surfaces, and mineralization of bones. Eight rat vertebrae, six rat tibiae, and eight minipig vertebrae were scanned using SR-CT at the synchrotron radiation facility Super Photon ring-8GeV (SPring-8). Images obtained using conventional micro-CT, scanning electron microscopy (SEM), and contact microradiography (CMR) were compared with the SR-CT images. SR-CT showed high image quality without visible partial volume effect. Three-dimensional SR-CT revealed shallow concavities in the bone surface, which were considered to correspond to osteoclastic resorption areas, as well as the connectivity, anisotropy, and shape (rod- or platelike) of trabeculae. Two-dimensional SR-CT showed different density along the surface of the trabecular bone, indicating the degree of bone mineralization. In conclusion, SR-CT seems to be a useful tool for delineating trabecular surfaces, evaluating bone mineralization, and revealing precise trabecular structure.
Ito
Masako
M
Department of Radiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852-8501 Nagasaki, Japan.
Ejiri
Sadakazu
S
Jinnai
Hiroshi
H
Kono
Jun
J
Ikeda
Satoshi
S
Nishida
Akifumi
A
Uesugi
Kentaro
K
Yagi
Naoto
N
Tanaka
Mikako
M
Hayashi
Kuniaki
K
D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging*; D002113:Calcification, Physiologic; D005260:Female; D023421:Models, Animal; D051381:Rats; D013131:Spine / Q000000981:diagnostic imaging; D017356:Synchrotrons; D013977:Tibia / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed / Q000379:methods*