Detailed kinetic analysis of a family 52 glycoside hydrolase: a beta-xylosidase from Geobacillus stearothermophilus.
Geobacillus stearothermophilus T-6 encodes for a beta-xylosidase (XynB2) from family 52 of glycoside hydrolases that was previously shown to hydrolyze its substrate with net retention of the anomeric configuration. XynB2 significantly prefers substrates with xylose as the glycone moiety and exhibits a typical bell-shaped pH dependence curve. Binding properties of xylobiose and xylotriose to the active site were measured using isothermal titration calorimetry (ITC). Binding reactions were enthalpy driven with xylobiose binding more tightly than xylotriose to the active site. The kinetic constants of XynB2 were measured for the hydrolysis of a variety of aryl beta-D-xylopyranoside substrates bearing different leaving groups. The Brønsted plot of log k(cat) versus the pK(a) value of the aglycon leaving group reveals a biphasic relationship, consistent with a double-displacement mechanism as expected for retaining glycoside hydrolases. Hydrolysis rates for substrates with poor leaving groups (pK(a) > 8) vary widely with the aglycon reactivity, indicating that, for these substrates, the bond cleavage is rate limiting. However, no such dependence is observed for more reactive substrates (pK(a) < 8), indicating that in this case hydrolysis of the xylosyl-enzyme intermediate is rate limiting. Secondary kinetic isotope effects suggest that the intermediate breakdown proceeds with modest oxocarbenium ion character at the transition state, and bond cleavage proceeds with even lower oxocarbenium ion character. Inhibition studies with several gluco analogue inhibitors could be measured since XynB2 has low, yet sufficient, activity toward 4-nitrophenyl beta-D-glucopyranose. As expected, inhibitors mimicking the proposed transition state structure, such as 1-deoxynojirimycin, bind with much higher affinity to XynB2 than ground state inhibitors.
Bravman
Tsafrir
T
Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Zolotnitsky
Gennady
G
Belakhov
Valery
V
Shoham
Gil
G
Henrissat
Bernard
B
Baasov
Timor
T
Shoham
Yuval
Y
D001406:Bacillaceae / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D003903:Deuterium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D013816:Thermodynamics; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism*
D001426:Bacterial Proteins; D014990:Xylans; D003903:Deuterium; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase