title: TcArf1: a Trypanosoma cruzi ADP-ribosylation factor.
abstract: Arfs (ADP-ribosylation factors) are conserved GTP-binding proteins involved in the control of coatomers assembling in budding vesicles in the eukaryotic secretory pathway and during some endocytic events. Here, we describe the gene for an Arf-homologue from the unicellular kinetoplastid parasite Trypanosoma cruzi, named TcArf1. TcArf1 is present in a discrete copy number in the T. cruzi genome and is expressed as a 1-kb transcript in the three main life forms of the parasite. Increased mRNA expression in epimastigotes may correlate with abundant protein biosynthesis and endocytosis in this stage.
authors.affiliation: Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: de Sá-Freire
authors.forename: José Luciano
authors.initials: JL
authors.lastname: Nepomuceno-Silva
authors.forename: Júlio César
authors.initials: JC
authors.lastname: da Paixão
authors.forename: Sérgio Maurício
authors.initials: SM
authors.lastname: de Mendonça
authors.forename: Luiz Dione Barbosa
authors.initials: LD
authors.lastname: de Melo
authors.forename: Ulisses Gazos
authors.initials: UG
authors.lastname: Lopes
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015698:Genomic Library; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D020823:ADP-Ribosylation Factor 1