Hard-food mastication suppresses complete Freund's adjuvant-induced nociception.
The effect of food hardness during mastication on nociceptive transmission in the spinal cord was studied by analyzing complete Freund's adjuvant (CFA) induced nocifensive behavior and Fos expression. The behavioral study showed that the shortening of the withdrawal latency following CFA injection into the hind paw was depressed after a change in the given food hardness from soft to hard. The depression of nocifensive behavior in the rats with hard food was reversed after i.v. injection of naloxone. Fos protein-like immunoreactive cells (Fos protein-LI cells) were expressed in the superficial and deep laminae of the L4-6 spinal dorsal horn after s.c. injection of CFA into the hind paw during soft food mastication. The number of Fos protein-LI cells was decreased in the rats with hard food mastication followed by soft food. This reduction of Fos protein-LI cells following change in food hardness was reversed after i.v. application of naloxone. Furthermore, the depression of Fos protein-LI cells following hard food intake was significantly inhibited after bilateral inferior alveolar nerve transection or bilateral ablation of the somatosensory cortex. These findings suggest that the change in food hardness during mastication might drive an opioid descending system through the trigeminal sensory pathway and somatosensory cortex resulting in an antinociceptive effect on chronic pain. However, IAN transection and cortical ablation did not induce 100% reversal of Fos expression, suggesting other than trigeminal sensory system may be involved in this phenomena, such as the pathway through the brainstem reticular formation.
Ogawa
A
A
Department of Dental Anesthesiology, Osaka University, Graduate School, Faculty of Dentistry, Suita, 565-0871, Osaka, Japan.
Morimoto
T
T
Hu
J W
JW
Tsuboi
Y
Y
Tashiro
A
A
Noguchi
K
K
Nakagawa
H
H
Iwata
K
K
D000818:Animals; D001522:Behavior, Animal; D002452:Cell Count; D003714:Denervation / Q000379:methods; D005502:Food; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007839:Functional Laterality; D006614:Hindlimb / Q000187:drug effects / Q000294:innervation; D008297:Male; D008340:Mandibular Nerve / Q000502:physiology / Q000601:surgery; D008409:Mastication; D009270:Naloxone / Q000008:administration & dosage; D009292:Narcotic Antagonists / Q000008:administration & dosage; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D013003:Somatosensory Cortex / Q000502:physiology / Q000601:surgery; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism; D013997:Time Factors
D009292:Narcotic Antagonists; D016760:Proto-Oncogene Proteins c-fos; D009270:Naloxone; D005620:Freund's Adjuvant