Stage-specific gene expression during Trypanosoma cruzi metacyclogenesis.
The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression in trypanosomatids. It may also improve the understanding of the mechanisms responsible for the pathology of Chagas disease, and could lead to the identification of new targets for chemotherapy of this disease. We have demonstrated that during metacyclogenesis the expression of several genes is controlled at the translational level by an alternative regulatory mechanism. This mechanism may involve the mobilization of mRNA to the translation machinery. We have been using self-made T. cruzi microarrays to investigate the role of polysomal mobilization in modulating gene expression during metacyclogenesis.
Avila
Andréa Rodrigues
AR
Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader, 3775, 81350-010, Curitiba, PR, Brasil.
Dallagiovanna
Bruno
B
Yamada-Ogatta
Sueli Fumie
SF
Monteiro-Góes
Viviane
V
Fragoso
Stênio Perdigão
SP
Krieger
Marco Aurélio
MA
Goldenberg
Samuel
S
D000818:Animals; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D008018:Life Cycle Stages / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity