Effects of different perfusates on functional parameters of isolated perfused dog kidneys.
BACKGROUND
The isolated perfused canine kidney has been established as a valid model for conducting both renal physiology and transplantation research. This model is of particular importance for developing new strategies to improve graft function after renal transplantation. In the present study, a newly developed method using isolated haemoperfused porcine kidneys was adapted for use in canine kidneys. In contrast to haemoperfusion, synthetic perfusion media can be standardized and can prevent the initiation of blood-mediated reperfusion reactions. Thus, an additional aim was to determine whether blood could be replaced by synthetic cell-free perfusion solutions.


METHODS
Canine kidneys (n = 30) were harvested from donors euthanized in veterinary practices for causes unrelated to the present study. The kidneys were isolated and perfused with autologous blood or cell-free synthetic electrolyte buffer (Tyrode solution). During perfusion, we monitored renal perfusate flow (RPF), glomerular filtration rate (GFR), electrolyte and glucose reabsorption, oxygen consumption and urine concentration.


RESULTS
Changes in perfusion medium did not affect the RPF. In contrast, GFR, urine concentration and oxygen consumption were significantly higher, whereas fractional excretion of sodium and glucose were significantly lower in blood- than in Tyrode-perfused kidneys.


CONCLUSIONS
This system offers a simple model for studying whole-organ functional alterations after acute renal ischaemia. Renal function indicators were below values reported during in vivo physiological conditions. These functions were better conserved when kidneys were perfused with autologous blood than with Tyrode.
Höchel
Joachim
J
Institute of Veterinary Physiology, Freie Universität Berlin, Berlin, Germany. hoechel@vetmed.fu-berlin.de
Lehmann
Dörte
D
Fehrenberg
Claudia
C
Unger
Volker
V
Groneberg
David A
DA
Grosse-Siestrup
Christian
C
D000818:Animals; D001769:Blood; D004285:Dogs; D066298:In Vitro Techniques; D007552:Isotonic Solutions / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000503:physiopathology*; D016030:Kidney Transplantation / Q000502:physiology; D023421:Models, Animal; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010477:Perfusion / Q000379:methods*; D015424:Reperfusion
D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C037692:Tyrode's solution