title: Smoking and atopy as determinants of sputum eosinophilia and bronchial hyper-responsiveness in adults with normal lung function.
abstract: Data concerning the determinants of sputum eosinophilia and bronchial hyper-responsiveness (BHR) in large cohorts of individuals with normal lung function are limited. Here, we assessed the occurrence of sputum eosinophilia and BHR and identified the risk factors for these variables in two populations living in North Karelia, Finland, and in Pitkäranta, the Republic of Karelia, Russia. These areas are geographically adjacent, but differ, however, fundamentally in major cultural, socioeconomical and lifestyle aspects. The study population comprised 790 Finns and 387 Russian, aged 25-54 years, who were randomly enrolled from the population registers. A methacholine challenge test to measure BHR was successfully performed in 581 (74%) Finns and 307 (79%) Russians with virtually normal lung function (FEV1 > 70% of predicted). Of these, induced sputum samples were obtained from 41% of the Finns and from 67% of the Russians. The proportion of current smokers was 27% among the former and 42% among the latter. Sputum eosinophilia was assessed using a semi-quantitative method, and total concentrations of sputum eosinophilic cationic protein (ECP) and myeloperoxidase (MPO) were measured using an immunoassay. Risk factors for BHR and sputum eosinophilia were identified with a regression analysis. The prevalence of sputum eosinophilia was 22% among the Finns and 19% among the Russians, and the respective figures for BHR were 14% and 13%. The median ECP concentration in sputum was significantly higher among the Russians as compared with the Finns (P<0.001), whereas for MPO, the difference did not achieve significance. Current smoking was significantly associated with both sputum eosinophilia and BHR in Russia (OR 3.1, 95% CI 1.2-7.6 for sputum eosinophilia, 2.8, 1.3-6.1 for BHR) and with BHR in Finland (2.1, 1.3-3.7). Atopy showed a tendency to be another risk factor for BHR in Finland (1.6, 0.98-2.6). In conclusion, sputum eosinophilia and BHR occurred commonly among the Finns and the Russians with normal lung function. Current smoking was significantly associated with BHR in both countries and additionally with sputum eosinophilia in Russia. Atopy was identified as a risk factor, albeit of borderline significance, for BHR in Finland only, suggesting that there may be differences in the aetiology and nature of BHR between the two countries.
authors.affiliation: Skin and Allergy Hospital, Helsinki University Central Hospital, Meilahdentie 2, 000250 Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Petäys
authors.forename: L
authors.initials: L
authors.lastname: von Hertzen
authors.forename: T
authors.initials: T
authors.lastname: Metso
authors.forename: P
authors.initials: P
authors.lastname: Rytilä
authors.forename: P
authors.initials: P
authors.lastname: Jousilahti
authors.forename: I
authors.initials: I
authors.lastname: Helenius
authors.forename: E
authors.initials: E
authors.lastname: Vartiainen
authors.forename: T
authors.initials: T
authors.lastname: Haahtela
mesh_terms: D000328:Adult; D001980:Bronchi / Q000187:drug effects; D001985:Bronchial Provocation Tests; D016084:Bronchoconstriction; D016085:Bronchoconstrictor Agents; D004802:Eosinophilia / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D004804:Eosinophils; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D008297:Male; D016210:Methacholine Chloride; D008875:Middle Aged; D012130:Respiratory Hypersensitivity / Q000473:pathology* / Q000503:physiopathology; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects* / Q000473:pathology / Q000503:physiopathology; D013183:Sputum / Q000166:cytology*
chemical_list: D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride