title: Preferential locomotion of leukemic cells towards laminin isoforms 8 and 10.
abstract: To identify the laminin isoforms of the basement membranes that could be implicated in the extravasation process of neoplastic lymphocytes, a number of purified laminins and one native renal laminin complex were comparatively investigated for their ability to promote migration of neoplastic lymphocytes in vitro. The identity/composition of a human placental laminin complex was asserted by combining immunochemical assays, sequence determination of tryptic peptides, and ultrastructural analysis to be composed predominantly of laminin-10 in which the coiled-coil C-terminal regions and the G globular domain of the alpha5 chain were preserved intact despite the enzymatic treatment used for its isolation. Lymphoma and leukemic cell lines failed to migrate towards laminin-4, -9, -11, moved poorly in response to laminin-1, -2/4, -5 and the renal laminin complex, but markedly locomoted towards the subendothelial laminin-8 and -10. The motility-promoting interaction with these latter laminins was interchangeably mediated by the alpha3beta1 and alpha6beta1 integrins. Lymphocyte locomotion on laminins assayed in the presence of cytokines was either reduced or enhanced suggesting that local cytokine milieu could further influence motility response.
authors.affiliation: Divisione di Oncologia Sperimentale 2, CRO-IRCCS, National Cancer Institute, CRO-IRCCS, Aviano, Aviano 3081, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Spessotto
authors.forename: Anna
authors.initials: A
authors.lastname: Gronkowska
authors.forename: Rainer
authors.initials: R
authors.lastname: Deutzmann
authors.forename: Roberto
authors.initials: R
authors.lastname: Perris
authors.forename: Alfonso
authors.initials: A
authors.lastname: Colombatti
mesh_terms: D001402:B-Lymphocytes / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000378:metabolism / Q000494:pharmacology; D019169:Jurkat Cells; D007797:Laminin / Q000302:isolation & purification / Q000378:metabolism*; D007938:Leukemia / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D010920:Placenta / Q000737:chemistry; D013379:Substrate Specificity; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D007378:Interleukins; D007797:Laminin; C119013:laminin 10; C110308:laminin 8