title: Focal length variability and protein leakage as tools for measuring photooxidative damage to the lens.
abstract: Hypericin is the ingredient used to standardize the popular over-the-counter antidepressant medication St. John's Wort. Because hypericin readily produces singlet oxygen and other excited state intermediates, it is a very efficient phototoxic agent in the eye that can potentially induce the development of the cataract photooxidative mechanism. Hypericin absorbs in the UV and visible ranges, binds to the lens crystallins (alpha, beta and gamma) and damages these proteins through a photooxidative mechanism. Effects were measured previously using fluorescence, UV and mass spectrometry. We report here two additional methods to monitor lens damage: (1) measuring focal length variability using a ScanTox instrument and (2) measuring protein leakage from the damaged lens. Because nonenzymic glycation results in free radical production, we chose to use elevated glucose concentrations as a convenient model for studying oxidative stress. To compare and contrast photooxidative damage against oxidative damage to the lens, we also measured the focal length variability and protein leakage induced by the presence of elevated glucose concentrations. We found that the total accumulated protein leakage was positively correlated (r = 0.9) with variability in focal length. Lenses treated with hypericin and irradiated with UVB had an increase in focal length variability as compared with the lenses that were only UVB-irradiated. Lenses without UVB irradiation had much lower focal length variability than irradiated lenses. For non-hypericin-treated lenses, UVB-irradiated lenses had a larger variability (4.58 mm) than the unirradiated lenses (1.78 mm). The lenses incubated in elevated glucose concentrations had a focal length variability (3.23 mm) equivalent to that of the unirradiated hypericin-treated lenses (3.54 mm). We conclude that photooxidative damage by hypericin results in changes in the optical properties of the lens, protein leakage and finally cataract formation. In contrast to this, high concentrations of glucose induced protein leakage but not changes in optical properties or the opacity associated with a cataract. This work provides further evidence that people should protect their eyes from intense sunlight when taking St. John's Wort.
authors.affiliation: Department of Biochemistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: Judit
authors.initials: J
authors.lastname: Wahlman
authors.forename: Maurice
authors.initials: M
authors.lastname: Hirst
authors.forename: J E
authors.initials: JE
authors.lastname: Roberts
authors.forename: Claire D
authors.initials: CD
authors.lastname: Prickett
authors.forename: John R
authors.initials: JR
authors.lastname: Trevithick
mesh_terms: D000818:Animals; D000873:Anthracenes; D002386:Cataract / Q000473:pathology; D002417:Cattle; D007908:Lens, Crystalline / Q000166:cytology / Q000473:pathology / Q000528:radiation effects*; D008027:Light; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D018384:Oxidative Stress; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D011506:Proteins / Q000032:analysis
chemical_list: D000873:Anthracenes; D011506:Proteins; D010569:Perylene; C004965:hypericin