title: Interaction between chondroitin-6-sulfate and Entamoeba histolytica as revealed by force spectroscopy.
abstract: We have observed by atomic force microscopy (AFM) the amoeba surface and probed the interaction force between Entamoeba histolytica and chondroitin-6-sulphate (C6S). We have used several substrates to adhere trophozoites. The best reproducibility in sample preparation was obtained with fibronectin-coated coverslips and when the cells were fixed with paraformaldehyde. The images obtained with the AFM showed that the trophozoite exhibits an irregular surface. Pseudopods and waving adhesion plaques could be observed. Force spectroscopy analysis showed that the trophozoite surface strongly interacts with C6S-functionalized tips. During cantilever retraction, attractive force peaks were observed at distances up to 1.3 microm above the trophozoite surface. Statistical analysis of the force distributions collected for five samples shown a reproducible 2.2 nN mean adhesion force. We observed a reduction of the adhesion force and of the interaction distance after addition of galactose to the buffer solution suggesting that the observed interaction is also Gal/GalNAc-lectin-mediated.
authors.affiliation: Departamento de Química, Universidade Federal do, Maranhão, MA, Brazil.
authors.forename: Gilza M P
authors.initials: GM
authors.lastname: Prazeres
authors.forename: Evander de J O
authors.initials: Ede J
authors.lastname: Batista
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
authors.forename: Paulo M
authors.initials: PM
authors.lastname: Bisch
authors.forename: Gilberto
authors.initials: G
authors.lastname: Weissmüller
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000378:metabolism*; D005353:Fibronectins; D018625:Microscopy, Atomic Force
chemical_list: D005353:Fibronectins; D002809:Chondroitin Sulfates