Mapping of viral RNA sequences required for assembly of peanut clump virus particles.
RNA sequences required for assembly into rod-shaped virions of RNA-1 and RNA-2 of Peanut clump virus (PCV) were mapped by testing the ability of different RNA-1 and -2 deletion mutants to be encapsidated in vivo in an RNase-resistant form. Encapsidation of RNA-1 was found to require a sequence domain in the 5'-proximal part of the P15 gene, the 3'-proximal gene of RNA-1. On the other hand, the subgenomic RNA which encodes P15 was not encapsidated, suggesting that other features of RNA-1 are important as well. Two sequences which could drive encapsidation of RNA-2 deletion mutants were located. One was in the 5'-proximal coat protein gene and the other in the P14 gene near the RNA 3' terminus. There were no obvious sequence homologies between the different assembly initiation sequences.
Hemmer
Odile
O
Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Dunoyer
Patrice
P
Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Richards
Kenneth
K
Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Fritsch
Christiane
C
Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D017353:Gene Deletion; D009710:Nucleotide Mapping; D010942:Plant Viruses / Q000502:physiology*; D012328:RNA Viruses / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics; D017421:Sequence Analysis; D014157:Transcription Factors / Q000235:genetics; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics; D019065:Virus Assembly / Q000235:genetics*
D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D012367:RNA, Viral; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins