Fluorescence behavior of Euglena photoreceptor.
To provide new information on the series of structural changes that Euglena photoreceptive proteins undergo inside the photoreceptor in response to light, we measured in vivo emission fluorescence spectra in the stable intermediates of its photocycle. Our emission spectra give a certain indication that fluorescent proteins are present in the Euglena photoreceptor and that they undergo a photocycle. On the basis of our data, we suggested that at least two stable intermediates, one of which is fluorescent, can be discriminated at room temperature and with our time resolution.
Evangelista
Valtere
V
Istituto di Biofisica CNR, Area della Ricerca di Pisa, via Moruzzi 1, Pisa, Italy. paolo.gualtieri@ib.pi.cnr.it
Passarelli
Vincenzo
V
Barsanti
Laura
L
Gualtieri
Paolo
P
D000818:Animals; D005055:Euglena / Q000737:chemistry* / Q000166:cytology; D005453:Fluorescence; D017956:Photoreceptor Cells, Invertebrate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence