[Effect of supporting substrates on the structure of DNA and DNA-trivaline complexes studied by atomic force microscopy].
Linear DNA, circular DNA, and circular DNA complexes with trivaline (TV), a synthetic oligopeptide, were imaged by atomic force microscopy (AFM) using mica as a conventional supporting substrate and modified highly ordered pyrolytic graphite (HOPG) as an alternative substrate. A method of modifying the HOPG surface was developed that enabled the adsorption of DNA and DNA-TV complexes onto this surface. On mica, both purified DNA and DNA-TV complexes were shown to undergo significant structural distortions: DNA molecules decrease in height and DNA-TP displays substantial changes in the shape of its circular compact structures. Use of the HOPG support helps preserve the structural integrity of the complexes and increase the measured height of DNA molecules up to 2 nm. AFM with the HOPG support was shown to efficiently reveal the particular points of the complexes where, according to known models of their organization, a great number of bent DNA fibers meet. These results provide additional information on DNA organization in its complexes with TV and are also of methodological interest, since the use of the modified HOPG may widen the possibilities of AFM in studying DNA and its complexes with various ligands.
Klinov
D V
DV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia. klinov@ibch.ru
Martynkina
L P
LP
Iurchenko
V Iu
VIu
Demin
V V
VV
Strel'tsov
S A
SA
Gerasimov
Iu A
IuA
Vengerov
Iu Iu
IuIu
D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D004270:DNA, Circular / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006108:Graphite / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
D000538:Aluminum Silicates; D004270:DNA, Circular; D009842:Oligopeptides; C025780:dansylhydrazide trivaline; D006108:Graphite; D004247:DNA; C011934:mica