Nine short tandem repeat loci analysis in aged semen stains using the AmpFLSTR Profiler Kit and description of a new vWA variant allele.
Nine short tandem repeat (STR) loci, D3S1358, D5S818, vWA, TH01, D13S317, TPOX, FGA, D7S820 and CSF1PO, were investigated in semen stains of various ages using the AmpFLSTR Profiler Kit. The nine STR loci were typed from semen stains stored for up to 25 years with the application of 1-10 ng DNA. This system provides a useful tool in medicolegal individualization of aged semen stains. During this investigation we found a new variant allele 18.1 at the vWA locus.
Kido
Akira
A
Department of Legal Medicine, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi-ken 409-3898, Japan. akido@res.yamanashi-med.ac.jp
Hara
Masaaki
M
Yamamoto
Yasuhisa
Y
Kameyama
Hiroshi
H
Susukida
Rie
R
Saito
Kazuyuki
K
Takada
Aya
A
Oya
Masakazu
M
D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012661:Semen / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D013997:Time Factors
D011933:Reagent Kits, Diagnostic