Cdyl: a new transcriptional co-repressor.
Cdyl (chromodomain-Y-like) is a chromodomain-containing protein that is predominantly expressed during mouse spermiogenesis. In its carboxy-terminal portion, there is a domain with homology to the coenzyme A (CoA) pocket of the enoyl-CoA hydratase/isomerase, which is shown here to be able to bind CoA and histone deacetylases (HDACs). It also efficiently represses transcription. Moreover, the binding of Hdac1 represses the ability of Cdyl to bind CoA, and a Cdyl-CoA interaction only occurs in the absence of HDACs. These data suggest that Cdyl is primarily a transcriptional co-repressor. However, the degradation of cellular Hdac1 and Hdac2, as observed here in the elongating spermatids, may provide an HDAC-free environment in which Cdyl could bind CoA and participate in the global chromatin remodelling that occurs in these cells.
Caron
Cécile
C
Laboratoire de Biologie Moléculaire et Cellulaire de la Différenciation--INSERM U309, Equipe Chromatine et Expression des Gènes, Institut Albert Bonniot, Faculté de Médecine de Grenoble, Domaine de la Merci, 38706 La Tronche Cedex, France.
Pivot-Pajot
Christophe
C
van Grunsven
Leo A
LA
Col
Edwige
E
Lestrat
Cécile
C
Rousseaux
Sophie
S
Khochbin
Saadi
S
D000595:Amino Acid Sequence; D000818:Animals; D056970:Co-Repressor Proteins; D003065:Coenzyme A / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000378:metabolism; D006836:Hydro-Lyases; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
D056970:Co-Repressor Proteins; D011506:Proteins; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases; C485563:Cdyl protein, mouse; D006836:Hydro-Lyases; D003065:Coenzyme A