Effect of siliconized latex urinary catheters on the activity of carbapenems against Pseudomonas aeruginosa strains with defined mutations in ampC, oprD, and genes coding for efflux systems.
The decreased activity of carbapenems against Pseudomonas aeruginosa in the presence of eluates from siliconized latex urinary catheters is related to the loss of OprD and the expression of a new outer membrane protein (OMP). To understand this phenomenon, the activity of carbapenems and quinolones against P. aeruginosa strains, some producing AmpC beta-lactamase (BL), OprD, MexA-MexB-OprM and MexE-MexF-OprN, were determined in Mueller-Hinton broth (MH) and in MH-containing eluates. OMP profiles and BL activities were also studied. Resistance to imipenem in P. aeruginosa grown in eluate is due to both the loss of OprD and expression of BL. For meropenem this phenomenon is related to both the loss of OprD and the integrity of MexA-MexB-OprM.
Conejo
M Carmen
MC
Department of Microbiology, School of Medicine, University of Seville, Apdo. 914, Seville 41080, Spain. cconejo@us.es
Martínez-Martínez
Luis
L
García
Isabel
I
Picabea
Leandro
L
Pascual
Alvaro
A
D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins; D018441:Biofilms; D015780:Carbapenems / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D007840:Latex; D008826:Microbial Sensitivity Tests; D009154:Mutation; D018272:Porins / Q000235:genetics; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012828:Silicones; D014546:Urinary Catheterization / Q000009:adverse effects* / Q000295:instrumentation; D014552:Urinary Tract Infections / Q000188:drug therapy* / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D007840:Latex; D018272:Porins; D012828:Silicones; C077157:OprD protein, Pseudomonas aeruginosa; C048795:AmpC beta-lactamases; D001618:beta-Lactamases