title: Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis.
abstract: Analyses of short tandem repeat (STR) loci are very useful for improving the accuracy of paternity determination. Combined use of several STRs makes this test even more accurate. We have devised a simple but effective method which consists of the co-amplification of two STR loci (vWF and HumTPO) in a single test tube, separation of the PCR products using discontinuous polyacrylamide gel electrophoresis, and detection using sensitive silver staining. An appropriate combination of PCR primer pairs gave a high resolution and good separation of the two STR bands in the same lane on the same gel. Many combinations of STR loci have potential usefulness in paternity and individualization testing or population genetic studies in forensic practice.
authors.affiliation: Department of Legal Medicine, Gunma University School of Medicine, Maebashi 371-8511, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashima
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Mogi
authors.forename: K
authors.initials: K
authors.lastname: Kishi