title: Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
abstract: We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science and molecular diagnostics.
authors.affiliation: QIAGEN GmbH, Max-Volmer Strasse 4, 40724 Hilden, Germany. Thomas.Hanselle@qiagen.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Hanselle
authors.forename: Michael
authors.initials: M
authors.lastname: Otte
authors.forename: Thomas
authors.initials: T
authors.lastname: Schnibbe
authors.forename: Emma
authors.initials: E
authors.lastname: Smythe
authors.forename: Frank
authors.initials: F
authors.lastname: Krieg-Schneider
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D006801:Humans; D009061:Mouth Mucosa / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012822:Silicon Dioxide; D020080:Tandem Repeat Sequences
chemical_list: D012822:Silicon Dioxide; D004247:DNA