Differential effect of chromogranin A fragments on invasion and growth of prostate cancer cells in vitro.
OBJECTIVE
To investigate the effect of various chromogranin A (CgA) fragments on the invasion, haptotactic migration, and growth of prostate cancer cells.


METHODS
We investigated the effect of five kinds of CgA fragments (79-115, 286-301, 324-341, 344-374, and 356-374) on the invasion of PC-3 and DU-145 prostate cancer cells through a reconstituted basement membrane (matrigel) and the haptotactic migration of these cells using a Transwell cell culture chamber assay. Cell growth was assessed by the WST-1 Cell Counting Kit.


RESULTS
CgA (79-115) inhibited the invasive ability of PC-3 and DU-145 cells (P = 0.035 and P = 0.037, respectively). CgA (79-115) also inhibited the haptotactic migration of these cells (P = 0.031 and P = 0.021). On the other hand, other CgA fragments had no significant effect. CgA (79-115) also inhibited the cell growth of PC-3 cells (P = 0.012) and DU-145 cells (P < 0.001). CgA (324-341), CgA (344-374), and CgA (356-374) inhibited the cell growth of DU-145 cells (P < 0.001, P < 0.001, and P < 0.001, respectively).


CONCLUSIONS
These results indicate that some CgA fragments may affect the invasion and growth of prostate cancer cells.
Nagakawa
Osamu
O
Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama, Japan.
Fujiuchi
Yasuyoshi
Y
Fuse
Hideki
H
Saiki
Ikuo
I
D001485:Basement Membrane / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins