Protective role of zinc-metallothionein (Zn-MT) in iron nitrilotriacetate (Fe-NTA)-induced renal oxidative damage.
Several studies have shown the role of thiol-rich proteins especially metallothionein (MT) in the therapeutic interventions against oxidative damage. Previously, we have provided strong evidence for the involvement of ROS in iron nitrilotriacetate (Fe-NTA)-induced renal toxicity, which may have relevance to its carcinogenicity. The purpose of this study was to evaluate the role of zinc metallothionein (Zn-MT) on the protection against Fe-NTA-induced renal oxidative damage. The results demonstrate that Zn-MT pretreatment provided protection against Fe-NTA-induced mortality in mice (40% protection). Similarly, Zn-MT pretreatment also provided protection against Fe-NTA-induced lipid peroxidation (26% inhibition, P < 0.001). It is proposed that Zn-MT protects kidney tissue against the noxious effect of Fe-NTA primarily by interference with lipid peroxides. It is concluded that Zn-MT may serve as an excellent physiological antioxidant against Fe-NTA-mediated renal oxidative damage.
Iqbal
Mohammad
M
Department of Pathological Research, Faculty of Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. m_iqbal2k@hotmail.com
Noor
Rana
R
Mizuno
Ryuichiro
R
Okada
Shigeru
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005290:Ferric Compounds / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000009:adverse effects; D008297:Male; D008668:Metallothionein / Q000494:pharmacology*; D051379:Mice; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D015032:Zinc / Q000378:metabolism
D000975:Antioxidants; D005290:Ferric Compounds; D008054:Lipid Peroxides; C021267:zinc thionein; D008668:Metallothionein; D015032:Zinc; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate