Behavior of SaOS-2 cells cultured on different titanium surfaces.
Surface properties may affect the clinical outcome of titanium dental implants. The aim of the present study was to investigate the effects of 3 different titanium surfaces-smooth (S), sandblasted (SB), and titanium plasma-sprayed (TPS)-on proliferation, differentiation, and apoptosis of human osteoblast-like cells, SaOS-2. Cell proliferation was significantly (p < 0.05) higher on the S surface, and synthesis of extracellular matrix proteins was more abundant on TPS and SB than on S surfaces. Analysis of integrin receptors showed a higher expression of alpha2, alpha5, alphaVbeta3, and ss1 on TPS as compared with SB and S surfaces. An increase in alkaline phosphatase activity was detected only on SB and TPS surfaces. Analysis of cell apoptosis did not demonstrate any significant difference among the 3 different surfaces. The results indicate that titanium surface topography affects proliferation and differentiation of osteoblast-like SaOS-2 cells, suggesting that surface properties might be important for bone response around dental implants in vivo.
Postiglione
L
L
Dept. of Cellular and Molecular Biology and Pathology, "L. Califano", Naples, Italy. lorposti@unina.it
Di Domenico
G
G
Ramaglia
L
L
Montagnani
S
S
Salzano
S
S
Di Meglio
F
F
Sbordone
L
L
Vitale
M
M
Rossi
G
G
D000469:Alkaline Phosphatase / Q000032:analysis; D017209:Apoptosis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000032:analysis; D003764:Dental Materials / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D016023:Integrins / Q000032:analysis; D010006:Osteoblasts / Q000166:cytology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; D016023:Integrins; D004247:DNA; D014025:Titanium; D000469:Alkaline Phosphatase