HLA-DQ polymorphisms modify the risk of thyroid autoimmunity in children with type 1 diabetes mellitus.
OBJECTIVE
Type 1 diabetes mellitus (DM1) is frequently accompanied by thyroid autoimmunity (TAI). The aims of the present study were to estimate the prevalence of TAI and to determine the contribution of HLA-DQA1 and -DQB1 polymorphisms to TAI susceptibility among children with DM1. PATIENTS AND METLHODS: Screening for TAI was performed in 285 children with DM1 by measuring autoantibodies against thyroid peroxidase (anti-TPO) and thyroglobulin (anti-Tg). HLA-DQA1 and -DQB1 were genotyped using PCR-SSP.


RESULTS
Repeated positivity of anti-TPO and/or anti-Tg was found in 45/285 children with DM1 (15.8%). The prevalence was significantly higher in girls than in boys (26.7% vs 6.7%; p<10(-5)). The HLA-DQB1*0302 allele conferred susceptibility to TAI in children with DM1 (OR 2.7, 95% CI 1.1-6.4), while the DQB1*05 alleles acted protectively (OR 0.2, CI 95% 0.08-0.7).


CONCLUSIONS
HLA-DQ polymorphisms significantly modify the risk of TAI in children with DM1.
Sumník
Z
Z
2nd Department of Pediatrics, 2nd Medical School, Charles University, Prague, Czech Republic. Zdenek.Sumnik@Lfmotol.cuni.cz
Drevínek
P
P
Snajderová
M
M
Kolousková
S
S
Sedláková
P
P
Pechová
M
M
Vavrinec
J
J
Cinek
O
O
D000293:Adolescent; D000483:Alleles; D001323:Autoantibodies / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D008297:Male; D010641:Phenotype; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic / Q000235:genetics; D018570:Risk Assessment; D013960:Thyroid Function Tests; D013967:Thyroiditis, Autoimmune / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology
D001323:Autoantibodies; D006683:HLA-DQ Antigens