Effect of acupuncture on nitric oxide synthase expression in cerebral cortex of streptozotocin-induced diabetic rats.
Effect of acupuncture on the expressions of nitric oxide synthase (NOS) and neuronal NOS (nNOS) in the cerebral cortex of streptozotocin (STZ)-induced diabetic rats was investigated. Animals were divided into four groups; the control group, the nondiabetic and acupunctured group, the STZ-induced-diabetes group, and the STZ-induced diabetic and acupunctured group. To produce the diabetic animal model, a single intraperitoneal injection of STZ (50 mg/kg) was given to each animal; animals of the nondiabetic groups received equivalent amounts of normal saline, also via intraperitoneal injection. From the results, acupuncture was shown to increase the numbers of nicotinamide adenine dinucleotide phosphate-diaphorase-positive and nNOS-positive neurons in STZ-induced diabetic rats. From the present study, it may be suggested that acupuncture modulates NOS and nNOS expressions in the cerebral cortex under diabetic conditions.
Jang
Mi-Hyeon
MH
Dept. of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
Shin
Min-Chul
MC
Kim
Young-Pyo
YP
Kim
Ee-Hwa
EH
Kim
Chang-Ju
CJ
D015670:Acupuncture Therapy / Q000379:methods; D000704:Analysis of Variance; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000378:metabolism / Q000628:therapy; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase