[Expression of Fas, FasL and IFN-gamma in gastric cancer].
OBJECTIVE
To study the expression of Fas, Fas ligand (FasL) and IFN-gamma in gastric cancer and its possible significance.


METHODS
Fifty-eight gastric paraffin wax embedded cancer tissues and fifty-three normal tissues adjacent gastric cancer were tested for the expression of Fas and FasL protein by immunohistochemistry and IFN-gamma mRNA by in situ hubridisation respectively.


RESULTS
The positive rate of Fas in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the tissues adjacent cancer(19.0% and 64.2%, respectively; chi 2 = 23.46, P = 0.00). The positive rate of FasL in cancer cells of gastric cancer tissues was significantly higher than that in gastric epithelial cells of the tissues adjacent cancer(63.8% and 45.3%, respectively; chi 2 = 3.83, P = 0.05). The positive rate of IFN-gamma in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the tissues adjacent cancer(0.0% and 49.1%, respectively; chi 2 = 37.16, P = 0.00).


CONCLUSIONS
The Fas-FasL system was unbalanced, and the expression of IFN-gamma was low in gastric cancer cells in this study. These may be related to the carcinogenesis of gastric epithelial cells and might be responsible for the immune escape of these cells.
Li
Zhaohui
Z
Department of General Surgery, Qilu Hospital of Shandong University, Ji'nan 250012, China.
Wang
Zhanmin
Z
Zhao
Zhilun
Z
Zhang
Yong
Y
Ke
Yang
Y
D004847:Epithelial Cells; D053222:Fas Ligand Protein / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism*; D012333:RNA, Messenger; D013274:Stomach Neoplasms / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C573415:IFNG protein, human; D012333:RNA, Messenger; D019014:fas Receptor; D007371:Interferon-gamma