title: Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements.
abstract: We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.
authors.affiliation: Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario, Canada K1N 6N5.
authors.forename: Lori
authors.initials: L
authors.lastname: Beach
authors.forename: Claude
authors.initials: C
authors.lastname: Schweitzer
authors.forename: J C
authors.initials: JC
authors.lastname: Scaiano
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D004996:Ethidium / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D009930:Organic Chemicals; D012996:Solutions / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C099476:PicoGreen; D012996:Solutions; D004247:DNA; D004996:Ethidium