title: Microbial ingrowth around single- and multi-component adhesives studied in vitro.
abstract: The aim of this study was to compare the in vitro microbial leakage in 4 micro-hybrid composites in combination with 4 single-component dental adhesives (Scotchbond 1/Z100 MP = group 1; Syntac Single-Component/Tetric Flow = group 3; OptiBond Solo/XRV Herculite = group 5; Solobond M/Arabesk Top = group 7) and 4 multi-component dental adhesives (Scotchbond Multi-Purpose/Z100 MP = group 2; Syntac/Tetric Flow = group 4; OptiBond FL/XRV Herculite = group 6; Solobond Plus/Arabesk Top = group 8). Ninety-four mixed standardized Class V cavities of human caries-free extracted premolars were filled with eight different composite adhesive systems using a one-layer (groups 1-4) or a two-layer technique (groups 5-8). After thermocycling and incubation in a broth culture of Streptococcus mutans and Lactobacillus acidophilus, followed by decalcification and staining, the extent and the type of microbial leakage were measured histologically. The extent of microbial leakage in the composite restorations was very low in all groups and there were no significant differences between adhesives. Z100 MP in combination with single- and multi-component adhesives showed a significantly higher microbial leakage than Tetric Flow systems (U test: p=0.037). XRV Herculite adhesive systems showed significantly less extensive microbial leakage than Arabesk Top adhesive systems (U test: p<0.001). The single-component dental adhesives achieved a marginal adaptation of composites comparable to that of multi-component adhesives in vitro.
authors.affiliation: Department of Conservative Dentistry, University Clinic Carl Gustav Carus, Fetscherstrasse 74, Dresden, Germany. susann.preussker@mailbox.tu-dresden.de
authors.forename: S
authors.initials: S
authors.lastname: Preussker
authors.forename: W
authors.initials: W
authors.lastname: Klimm
authors.forename: M
authors.initials: M
authors.lastname: Pöschmann
authors.forename: R
authors.initials: R
authors.lastname: Koch
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D003737:Dental Cavity Preparation / Q000145:classification; D003763:Dental Leakage / Q000382:microbiology; D018772:Dental Marginal Adaptation; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D007779:Lactobacillus acidophilus / Q000254:growth & development*; D008689:Methacrylates / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012822:Silicon Dioxide; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000254:growth & development*; D013499:Surface Properties; D015040:Zirconium
chemical_list: D000269:Adhesives; C425826:Arabesk; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C469607:OptiBond FL; C428805:OptiBond SOLO; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C425825:Solobond M; C078484:Syntac dentine adhesive; C083568:Z100 composite resin; C452226:tetric flow composite resin; C065770:Herculite XR; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; C041330:Scotchbond; D015040:Zirconium