title: Real-time PCR as a versatile tool for investigating the susceptibility of human herpesvirus 6 to antiviral agents.
abstract: A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.
authors.affiliation: Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Macé
authors.forename: Chaysavanh
authors.initials: C
authors.lastname: Manichanh
authors.forename: Pascale
authors.initials: P
authors.lastname: Bonnafous
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Précigout
authors.forename: David
authors.initials: D
authors.lastname: Boutolleau
authors.forename: Agnès
authors.initials: A
authors.lastname: Gautheret-Dejean
authors.forename: Henri
authors.initials: H
authors.lastname: Agut
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D005434:Flow Cytometry; D015654:Herpesvirus 6, Human / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral