title: [Core promoter mutations of HBV isolated from patients with chronic hepatitis B in Guangxi].
abstract: OBJECTIVE
  To explore the relationship between HBV core promoter mutations and liver damage or HBeAg status.
  
  
  METHODS
  Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter in 59 sera from patients with chronic hepatitis B in Guangxi, then the HBV DNA positive products were sequenced by direct sequencing.
  
  
  RESULTS
  The HBV DNA positive rate of was 59.3%(35/59). All the patients were infected by mutants. The commonest mutation was the double mutation (A --> T at nt1762 and G --> A at nt1764), counting for 57.1% (20/35). The next was C --> G at nt1799, counting for 54.4% (19/35), but this was no function. A --> G at nt1752 (resulting in isoleucine to valine) was seen in 37.1% (13/35) of the HBV DNA positive patients, and T --> C at nt1753 was seen in 20% (7/35). The significant difference in the frequency of T1762A1764 mutant was found between HBeAg positive patients (31.3%) and negative patients (79.0%).
  
  
  CONCLUSIONS
  HBV core promoter mutations are common among patients with chronic hepatitis B in Guangxi. T1762A1764 mutant is associated with HBeAg status and chronic hepatitis.
authors.affiliation: Centre for Diseases Control of Guangxi Zhuangzu Autonomous Region, Nanning 530021, China.
authors.forename: Zhong-liao
authors.initials: ZL
authors.lastname: Fang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhuang
authors.forename: Xian-min
authors.initials: XM
authors.lastname: Ge
authors.forename: Jin-ye
authors.initials: JY
authors.lastname: Yang
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.forename: Rong-cheng
authors.initials: RC
authors.lastname: Li
authors.forename: Roger
authors.initials: R
authors.lastname: Ling
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Harrison
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens