Antimicrobial peptides containing arginine.
Tetradecapeptides (RLARLAR)2, D-(RLARLAR)2, (RLARLAA)2, and (RLGRLGR)2 were synthesized by a solid phase method using Fmoc-amino acids. The antibacterial activity of the synthesized peptides was studied against Escherichia coli cells. The minimum inhibitory concentration (MIC) was, correspondingly, 3, 1, 3, and 12 micro M, which is comparable with MIC of such natural antimicrobial peptides as temporin, magainin, and dermaseptin. It was found that all of the synthesized peptides have no effect on human erythrocytes and rat thymocytes. The peptides form alpha-helices in 30% trifluoroethanol and in 2.5 mM SDS, which have amphipathic structure.
Ryadnov
M G
MG
Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.
Degtyareva
O V
OV
Kashparov
I A
IA
Mitin
Yu V
YV
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology; D001120:Arginine / Q000737:chemistry* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects
D029845:Amphibian Proteins; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; D011506:Proteins; C104501:temporin; C070905:dermaseptin; D001120:Arginine