Rat cerebral serine racemase: amino acid deletion and truncation at carboxy terminus.
Since mouse serine racemase had an amino acid deletion compared with human enzyme, rat enzyme was examined to see whether it had the same deletion. The nucleotide sequence of cDNA encoding rat serine racemase was determined. Deduced amino acid sequence of rat serine racemase had high homology with that of mouse and human enzyme. However, the rat enzyme had the same amino acid deletion as the mouse enzyme. In addition, the rat serine racemase was truncated at the carboxyl terminus and six amino acid residues were less than the other two enzymes. Northern hybridization detected serine racemase transcripts in many tissues.
Konno
Ryuichi
R
Department of Microbiology, Dokkyo University School of Medicine, Mibu, 321-0293 Tochigi, Japan. konno@dokkyomed.ac.jp
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000201:enzymology*; D018076:DNA, Complementary / Q000032:analysis*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D019998:Racemases and Epimerases / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D000099300:Serine Racemase
D018076:DNA, Complementary; D019998:Racemases and Epimerases; D000099300:Serine Racemase