[Study on interaction between the metal ion in the active site of cytochrome C and trace elements by ultraviolet spectroscopy].
The direct interaction between the metal ion in the active center of cytochrome C(Fecyt-C) and four trace elements in pH 7.1 phosphate buffer by UV spectrometry has been studied. As a result, part of the metal ion Fe(II) in Fecyt-C was replaced and the corresponding cytochrome C derivatives were produced. The Soret absorption maximum is blue-shifted and the absorption coefficient of the alpha band relative to that of the beta band is decreased in comparison to the Fecyt-C.
Jia
Y
Y
Department of Chemistry and Chemical Engineering, Dalian University, 116622 Dalian.
Zheng
X
X
Hu
J
J
D003300:Copper / Q000737:chemistry; D045304:Cytochromes c / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014131:Trace Elements / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
D005296:Ferrous Compounds; D014131:Trace Elements; D003300:Copper; D009532:Nickel; D045304:Cytochromes c; D007501:Iron; D015032:Zinc