Systemic cytokine responses in patients with influenza-associated encephalopathy.
Influenza-associated encephalopathy, a severe neurologic complication of influenza, is being reported more frequently in Japan. We investigated the transcription of cytokine genes in peripheral blood leukocytes and compared patients with influenza and with encephalopathy or febrile convulsions and patients with influenza but without neurologic complications. A quantitative polymerase chain reaction (PCR) revealed that transcription of the interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha genes was up-regulated to a greater extent in patients with encephalopathy than in those without neurologic complications. Plasma IL-6 levels also were higher in patients with encephalopathy, although the difference was marginal. Viral RNA in throat swabs was quantified using a real-time quantitative PCR. The virus load was similar among patients with encephalopathy or febrile convulsions or without neurologic complications. Furthermore, virus load was not correlated with either the transcription of cytokine genes or plasma cytokine concentrations. These results suggest that influenza-associated encephalopathy might be a consequence of systemic immune responses.
Kawada
Jun-ichi
J
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan. k-kawada@qa2.so-net.ne.jp
Kimura
Hiroshi
H
Ito
Yoshinori
Y
Hara
Shinya
S
Iriyama
Masahiro
M
Yoshikawa
Tetsushi
T
Morishima
Tsuneo
T
D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D018792:Encephalitis, Viral / Q000276:immunology* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000276:immunology; D009981:Influenza B virus / Q000276:immunology; D007251:Influenza, Human / Q000150:complications* / Q000276:immunology*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D012367:RNA, Viral / Q000097:blood; D003294:Seizures, Febrile / Q000276:immunology* / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D019562:Viral Load
D016207:Cytokines; D015850:Interleukin-6; D012367:RNA, Viral; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10