DNA damage in digestive gland and mantle tissue of the mussel Perna perna.
Data concerning the susceptibility of DNA to damage by reactive oxygen and nitrogen species and other endogenous compounds produced by physiological stress in marine organisms is lacking, especially in bivalve mollusks. In this article, we analyzed the background levels of lipid peroxidation (malondialdehyde, MDA), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-epsilon dGuo) in digestive gland and mantle tissue of mussels Perna perna collected at a cultivation zone in Florianópolis (Santa Catarina, Brazil). The present data point to the possibility of the use of both 8-oxodGuo and 1,N2-epsilon dGuo as complementary indicators of oxidative stress processes in mussels. A sensitive method coupling high performance liquid chromatography to mass spectrometry was applied for the detection of 1,N2-epsilon dGuo in mussel tissues.
de Almeida
Eduardo Alves
EA
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26.077, Av. Prof. Lineu Prestes 748, São Paulo, SP, CEP 05513-970, Brazil.
Marques
Sabrina de Almeida
Sde A
Klitzke
Clécio Fernando
CF
Bainy
Afonso Celso Dias
AC
de Medeiros
Marisa Helena Gennari
MH
Di Mascio
Paolo
P
Loureiro
Ana Paula de Melo
AP
D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism
D017382:Reactive Oxygen Species