title: Streptomyces lividans contains a minimal functional signal recognition particle that is involved in protein secretion.
abstract: The bacterial version of the mammalian signal recognition particle (SRP) is well conserved and essential to all known bacteria. The genes for the Streptomyces lividans SRP components have been cloned and characterized. FtsY resembles the mammalian SRP receptor and the S. lividans SRP consists of Ffh, a homologue of the mammalian SRP54 protein, and scRNA, which is a small size RNA of 82 nt in length. Co-immunoprecipitation studies confirmed that Ffh and scRNA are probably the only components of the S. lividans SRP and that pre-agarase can co-immunoprecipitate with Ffh, suggesting that the SRP is involved in targeting secretory proteins.
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Arantxa
authors.initials: A
authors.lastname: Palacín
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: de la Fuente
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Valle
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Rivas
authors.affiliation: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D020733:RNA, Small Cytoplasmic; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D018000:Receptors, Peptide / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D018271:Signal Recognition Particle / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C087406:FtsY protein, Bacteria; D020733:RNA, Small Cytoplasmic; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D011993:Recombinant Fusion Proteins; D018271:Signal Recognition Particle; C036318:signal peptide receptor