Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples.
The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.
Nunes
Cáris Maroni
CM
Department of Animal Health and Production, Universidade Estadual Paulista Júlio de Mesquita Filho, Curso de Medicina Veterinária, UNESP, campus Araçatuba, Rua Clóvis Pestana, 793, Jd. D. Amélia, Araçatuba, SP 16050-680, Brazil. caris@fmva.unesp.br
Lima
Luiz Gustavo Ferraz
LG
Manoel
Camila Santos
CS
Pereira
Rodrigo Norberto
RN
Nakano
Mauro Massaharu
MM
Garcia
José Fernando
JF
D000818:Animals; D002353:Carrier State / Q000175:diagnosis / Q000469:parasitology; D017931:DNA Primers; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D041202:Taenia saginata / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D017931:DNA Primers; D018509:DNA, Helminth