title: Modulation of neutrophil adhesion to vascular endothelial cells in rat experimental otitis media treated with a macrolide.
abstract: OBJECTIVE
  We previously reported that erythromycin (EM) affected the adhesion molecules that interact with neutrophils and inhibited neutrophil infiltration in a rat experimental otitis media model.
  
  
  OBJECTIVE
  The purpose of the present study was to examine whether EM affects the adhesion of rat neutrophils to endothelial cells.
  
  
  METHODS
  The adhesion of neutrophils to WK-5 endothelial cells was evaluated. One group of rats was given EM for 2 weeks, while the other group received no treatment. The adhesion of peripheral-blood neutrophils and neutrophils accumulated in the middle ear from these two groups were compared.
  
  
  RESULTS
  Two-week EM pretreatment inhibited adhesion of neutrophils to WK-5 cells. The adhesion of neutrophils accumulated in the middle ear cavity to WK-5 cells was significantly higher than that of peripheral-blood neutrophils from control rats.
  
  
  CONCLUSIONS
  The results suggest that EM affects the adhesion of neutrophils to endothelial cells.
authors.affiliation: Department of Otorhinolaryngology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyu-ku, Tokyo 113-8421, Japan. fenomoto@med.juntendo.ac.jp
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Enomoto
authors.forename: Ryutaku
authors.initials: R
authors.lastname: Kin
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kataoka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Hindenori
authors.initials: H
authors.lastname: Yokoi
authors.forename: Masato
authors.initials: M
authors.lastname: Fujimori
authors.forename: Isao
authors.initials: I
authors.lastname: Nagaoka
authors.forename: Ginichirou
authors.initials: G
authors.lastname: Ichikawa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology* / Q000627:therapeutic use; D019041:L-Selectin / Q000032:analysis / Q000187:drug effects; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects*; D010033:Otitis Media / Q000188:drug therapy*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000900:Anti-Bacterial Agents; D016177:Macrophage-1 Antigen; D019041:L-Selectin; D004917:Erythromycin