Gene expression and tyrosine kinase activity of insulin receptor in uterine leiomyoma and matched myometrium.
OBJECTIVE
Our objective was to determine the insulin receptor (IR) mRNA levels and IR tyrosine kinase activity in normal myometrium and leiomyoma.


METHODS
Experimental study.


METHODS
Academic research center.


METHODS
Five women with leiomyoma submitted to hysterectomy.


METHODS
Plasma membrane fraction of human myometrium and leiomyoma were prepared and samples were incubated with and without insulin. mRNA was isolated and RT-PCR with specific primers was performed.


METHODS
Western blots of plasma membranes incubated with and without insulin were performed. Chemoluminescent methods followed by densitometry were used to assess IR autophosphorylation. RT-PCR with specific primers for IR gene sequences was used to determine IR mRNA levels.


RESULTS
IR mRNA levels in myometrium (0.634+/-0.038) and in leiomyoma (0.649+/-0.047; p=0.813) were not different. The degree of insulin-stimulated IR autophosphorylation (relative optical density of the 95 kDa band) was also not different between myometrium (1.496+/-0.310) and leiomyoma (1.593+/-0.129; p=0.650).


CONCLUSIONS
There was no difference in IR receptor expression and IR autophosphorylation between normal myometrium and leiomyoma. Other steps of insulin signaling chain may participate in the altered proliferation of leiomyomas.
Toscani
Guilherme Kirjner
GK
Departamento de Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Chaves
Eunice Martin
EM
Cervi
Fabíola Lara
FL
Tavares
Marcelo Belmonte
MB
Silva
Ilma Simoni Brum da
IS
von Eye Corleta
Helena
H
Capp
Edison
E
D015153:Blotting, Western; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000378:metabolism*
D017931:DNA Primers; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin