[Study on determination of protein with Arsenazo I using resonance light scattering technique].
A new RLS (Resonance light scattering) probe is presented in this paper. In the Britton-Robinson buffer at pH 3.29, Arsenazo I combines with proteins by intermolecular forces, resulted in an enhanced Rayleigh light scattering. The RLS intensity reaches maximum at 400 nm and is proportional to the concentration of protein. A novel method for the determination of micro-amount of protein is developed. The assay is simple, rapid and sensitive with a detection limit at 60 ng.mL-1 and a linear range up to 18 micrograms.mL-1. This method is applied to the determination of human serum protein, and the results, compared to Bradford method, is satisfactory. The interaction of Arsenazo I with BSA, gamma-G, oval albumin, lysozyme, pepsin are investigated and the mechanism of RLS is also discussed.
Li
Shu-wei
SW
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Li
Na
N
Zhao
Feng-lin
FL
Li
Ke-an
KA
D001150:Arsenazo III; D004527:Egg Proteins / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D008027:Light; D010777:Photochemistry; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D005719:gamma-Globulins / Q000032:analysis
D004527:Egg Proteins; D007202:Indicators and Reagents; D011506:Proteins; D005719:gamma-Globulins; D001150:Arsenazo III; D012710:Serum Albumin, Bovine; C035803:arsenazo I