Mouse blastocyst vitrification compared with the conventional slow-freezing method.
To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrification and slow-freezing were 68.33 and 65.52 per cent (p = 0.89), whereas hatching rates were 51.22 and 44.74 per cent, respectively (p = 0.64). Therefore, vitrification of blastocyst-stage-embryos may be a useful, economic method for freezing the excess blastocysts in some centers where blastocysts are routinely transferred.
Tharnprisarn
W
W
Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Suwajanakorn
S
S
Sereepapong
W
W
Pruksananonda
K
K
Boonyakasemsanti
W
W
Virutamasen
P
P
Ahnonkitpanit
V
V
Chompurat
D
D
Numchaisrika
P
P
D000818:Animals; D001755:Blastocyst; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013997:Time Factors