Characterization and property of DNA incorporated bilayer lipid membranes.
Calf-thymus DNA-incorporated bilayer lipid membranes supported on a glassy carbon (GC) electrode was prepared by making layers of phosphatidylcholine dimyristoyl (DMPC) on GC electrode. DNA in the BLM was characterized by cyclic voltammetry, IR and AFM, and lipid layers formed on the GC electrode were demonstrated to be a bilayer lipid membrane by electrochemical impedance experiment. In IR and AFM experiments the findings indicated that DNA was incorporated into BLM. The ion channel of bilayer lipid membranes incorporated was studied. The result showed that the ion channel was opened in the presence of the stimulus quinacrine. In the absence of quinacrine the channel was switched. The process can repeat itself many times. The impedance spectroscopy measurements demonstrate that the stimulus quinacrine opens the channel for permeation of marker ion. The mechanism of forming an ion channel was investigated.
Tong
Yuehong
Y
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Han
Xiaojun
X
Song
Yonghai
Y
Jiang
Junguang
J
Wang
Erkang
E
D000818:Animals; D002244:Carbon / Q000737:chemistry; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D007473:Ion Channels / Q000737:chemistry / Q000187:drug effects; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011796:Quinacrine / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis / Q000379:methods
D020099:Coated Materials, Biocompatible; D005292:Ferricyanides; D007473:Ion Channels; D008051:Lipid Bilayers; D002244:Carbon; D004247:DNA; C427203:calf thymus DNA; D011796:Quinacrine; D004134:Dimyristoylphosphatidylcholine