[Secondary Raman spectrum of beta-carotene molecule in living leaf of French phoenix tree].
Under visible incidence light 514.5 nm, the Raman scattering spectrum from the beta-carotene molecule in the leaf was directly obtained after it was immediately collected from French phoenix tree without any preparing the sample but cleaning. It is very easy to collect the secondary Raman lines addition to the first Raman spectrum in situ by micro Raman. By careful comparing and analyzing the Raman lines between 2,000-3,100 cm-1 and below 2,000 cm-1 regions, we obtained the correlated relation of the first and secondary Raman lines. The study results indicated that there is no damage to the structure and configuration of beta-carotene molecule in the live leaf by controlling laser power on the sample surface and integrating time for Raman signal, but large power laser or long time irradiation on the live sample would cause very strong fluorescence background in Raman spectrum which indicated that there is a photo damage in the center of photo reaction. The Micro Raman would become one of possible in situ methods for investigating live plant molecules growing up in different environment. At last we proposed and discussed the advantages and limits in micro Raman when it is applied to investigating live molecules in botany field.
Zhao
Jin-tao
JT
Dept. of Applied Physics, School of Science and Arts, Hangzhou Institute of Electronic Engineering, Hangzhou 310037, China.
Zhang
Peng-xiang
PX
Xu
Cun-ying
CY
D019684:Magnoliopsida / Q000737:chemistry; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D014197:Trees / Q000737:chemistry*; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene