A phage-displayed peptide can inhibit infection by white spot syndrome virus of shrimp.
White spot disease, caused by white spot syndrome virus (WSSV), results in devastating losses to the shrimp farming industry around the world, and no effective treatments have been found. Control focuses on exclusion of the virus from culture ponds but, once introduced, spread is often rapid and uncontrollable. The purpose of this study was to select a phage-displayed peptide that might be able to prevent WSSV infection. A 10-mer phage display peptide library (titre 7.2 x 10(7)) was constructed and screened against immobilized WSSV. Selected peptides were assessed for specificity and efficiency of inhibition of virus infection. Of four peptides that specifically bound to WSSV one, designated 2E6, had a high specificity and blocked virus infection, with the possible critical motif for virus inhibition being VAVNNSY. The results suggest that peptide 2E6 has potential for exploitation as an antiviral peptide drug.
Yi
Guohua
G
Department of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
Qian
Juan
J
Department of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
Wang
Zhiming
Z
Department of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
Qi
Yipeng
Y
Department of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
D020816:Amino Acid Motifs; D000818:Animals; D003400:Astacoidea / Q000821:virology; D018929:Cell Culture Techniques; D004267:DNA Viruses / Q000187:drug effects; D033364:Decapoda / Q000187:drug effects / Q000821:virology*; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology; D013045:Species Specificity
D019151:Peptide Library; D010455:Peptides