title: [Effect of vitamin E on myosin light chain kinase activity and endothelial permeability of the artery in atherosclerotic rabbit].
abstract: OBJECTIVE
  To study the effect of vitamin E (Vit E) on the myosin light chain kinase(MLCK) activity and the endothelial permeability of the artery in atherosclerotic rabbits.
  
  
  METHODS
  The MLCK activity of rabbit artery was measured by incorporation of gamma-(32)P. The endothelial permeability was accessed by immunofluorescence.
  
  
  RESULTS
  The model of atherosclerosis was established after rabbits were fed with cholesterol for four weeks. The activity of MLCK increased markedly, and there was significantly statistical difference compared with the normal control (P<0.05). When the rabbits were fed with cholesterol for twelve weeks or with cholesterol and Vit E for twelve weeks, the activity of MLCK did not change markedly, and there was no statistical difference compared with the normal control, respectively (P>0.05). The permeability of arterial wall was increased after the rabbits were fed with cholesterol for four weeks, and the permeability increased even more obviously after the rabbits were fed with cholesterol for twelve weeks. The permeability appeared to be decreased when Vit E was added into the cholesterol feeding.
  
  
  CONCLUSIONS
  The change in integrity of arterial wall may be associated with the increase of the activity of MLCK. Vit E may decrease the MLCK activity. Vit E may decrease the endothelial permeability of atherosclerotic rabbits.
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui, China.
authors.forename: Hua-qing
authors.initials: HQ
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Ren
authors.forename: Jiang-xia
authors.initials: JX
authors.lastname: Xiong
authors.forename: Ruo-lei
authors.initials: RL
authors.lastname: Hu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Su-mei
authors.initials: SM
authors.lastname: Zhang
authors.forename: Hui-lan
authors.initials: HL
authors.lastname: Liu
authors.forename: Zhi-kui
authors.initials: ZK
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Shu-yu
authors.initials: SY
authors.lastname: Gui
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001158:Arteries; D004730:Endothelium, Vascular / Q000187:drug effects; D006801:Humans; D009219:Myosin-Light-Chain Kinase / Q000187:drug effects*; D010539:Permeability; D011817:Rabbits; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D014815:Vitamins; D014810:Vitamin E; D009219:Myosin-Light-Chain Kinase