High-speed genotyping of CYP1A2*1F mutation with fluorescent hybridization probes using the LightCycler.
The polymorphism CYP1A2*1F of the cytochrome 1A2 gene affects the inducibility of this cytochrome, which is involved in the metabolism of many commonly used drugs. We developed a rapid-cycle polymerase chain reaction (PCR) and fluorescent probe melting curve analysis on the LightCycler instrument for identification of this mutation. This new method was confirmed by genotyping 101 samples in parallel to restriction enzyme digestion. The 100% concordance of both methods showed the reliability of our high-speed genotyping assay, which is suitable for large epidemiological studies or routine clinical use.
Popp
Johannes
J
Institute for Clinical Chemistry and Pathobiochemistry, Munich University of Technology, Klinikum rechts der Isar, D-81675 Munich, Germany.
Messner
Barbara
B
Steimer
Werner
W
D000483:Alleles; D019388:Cytochrome P-450 CYP1A2 / Q000097:blood / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
D015342:DNA Probes; D019388:Cytochrome P-450 CYP1A2