Effect of different doses of cyanamide on striatal salsolinol formation after ethanol treatment.
To assess the dose-dependent effect of cyanamide (CY, a potent aldehyde dehydrogenase inhibitor) on salsolinol release in the striatum, rats were treated with CY (25, 50 and 100 mg/kg) plus ethanol (EtOH,1 g/kg) intraperitoneally. Striatal salsolinol was detected using in vivo microdialysis coupled with high-performance liquid chromatography with an electrochemical detector in free-moving rats, and blood acetaldehyde (AcH) and EtOH were detected using the head-space gas chromatographic method. With the increase in the doses of CY following EtOH, the peak concentrations of striatal salsolinol and blood AcH were increased significantly. Our study indicated that the magnitude of striatal salsolinol levels may depend on the concentration of blood AcH, and that there is a correlation between the blood AcH and striatal salsolinol.
Jamal
Mostofa
M
Department of Forensic Medicine, Faculty of Medicine, Kagawa Medical University, 1750-1 Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. jamal@kms.ac.jp
Ameno
Kiyoshi
K
Ameno
Setsuko
S
Okada
Naoko
N
Ijiri
Iwao
I
D000079:Acetaldehyde / Q000097:blood; D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D003484:Cyanamide / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D007263:Infusions, Parenteral; D007546:Isoquinolines / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D007546:Isoquinolines; D000431:Ethanol; D003484:Cyanamide; C036617:salsolinol; D000079:Acetaldehyde