title: The activity of antioxidant enzymes in Arabidopsis thaliana exposed to colchicine and H2O2.
abstract: Studies on the possible interference of colchicine and H2O2 with the activity of some antioxidant enzymes were carried out on Arabidopsis thaliana v. Columbia grown in Murashige and Skooge nutrient medium. Measurements of superoxide dismutase (SOD), guaiacol peroxidase (POX), ascorbate peroxidase (APX) and catalase (CAT) activities were conducted spectrophotometrically. In the presence of colchicine, SOD activity increased, while CAT, APX and POX activities decreased. Inhibitory H2O2 effects on the activity of the enzymes were found. Colchicine pre-treatment resulted in an increase in CAT activity and a further increase in SOD activity in plants treated with H2O2.
authors.affiliation: Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Drazkiewicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Skózyńska-Polit
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wanke
authors.forename: Ewa
authors.initials: E
authors.lastname: Swiezewska
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology*; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000032:analysis; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D009195:Peroxidase / Q000032:analysis; D010544:Peroxidases / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D016877:Oxidants; D006861:Hydrogen Peroxide; D010544:Peroxidases; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D003078:Colchicine