[Preparation and biological activities of monoclonal antibody against p185erbB2].
OBJECTIVE
To produce anti- p185erbB2 monoclonal antibody and investigate its biological activities on cancer cells that overexpress p185erbB2.


METHODS
BALB/c mice were immunized with NIH3T3-erbB2 cell to prepare the anti- p185erbB2 monoclonal antibody. The specificity of the monoclonal antibody 1H3 was identified with ELISA, Western blot, immunoprecipitation and immunohistochemistry assays. The effects of monoclonal antibody 1H3 on the proliferation of cell lines that overexpressed p185erbB2 were measured by MTT assay.


RESULTS
One strain of monoclonal antibody1H3 was obtained. In ELISA assay, 1H3 specifically reacted with cells overexpressing p185erbB2, but had no reaction with cells not overexpressing p185erbB2. The specificity of the monoclonal antibody 1H3 was verified by immunoprecipitation, Western blot and immunohistochemistry techniques. MTT assay was used to confirm that 1H3 could inhibit the growth of cells that overexpressed p185erbB2 to different extent.


CONCLUSIONS
The monoclonal antibody 1H3 obtained can specifically bind to p185erbB2 and significantly inhibit proliferation of cells overexpressing p185erbB2.
Han
Mei
M
Department of Biochemistry and Molecular Biology, Peking University School of Oncology, Beijing 100034, China.
Jiang
Beihai
B
Wu
Jian
J
Shou
Chengchao
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018719:Erb-b2 Receptor Tyrosine Kinases