Cloning and expression of alpha-D-glucosidase and N-acetyl-beta-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus).
Screening a genomic library of Tannerella forsythensis (Bacteroides forsythus), using synthetic substrates conjugated to a fluorogen, 4-methylumbelliferone identified two glycosidase genes, which encode alpha-D-glucosidase and N-acetyl-beta-D-glucosaminidase, respectively. The alpha-D-glucosidase has a Mr of 81,141 and is homologous to an alpha-D-glucosidase from Bacteroides thetaiotaomicron. The N-acetyl-beta-D-glucosaminidase has a Mr of 87,787 and is homologous to an N-acetyl-beta-D-glucosaminidase in Porphyromonas gingivalis W83.
Hughes
C V
CV
Department of Pediatric Dentistry, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA.
Malki
G
G
Loo
C Y
CY
Tanner
A C R
AC
Ganeshkumar
N
N
D000118:Acetylglucosaminidase / Q000235:genetics*; D001426:Bacterial Proteins; D001439:Bacteroides / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D005456:Fluorescent Dyes; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016680:Genome, Bacterial; D006801:Humans; D006923:Hymecromone; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010510:Periodontal Diseases / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D000520:alpha-Glucosidases / Q000235:genetics*
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; C484346:RagB protein, bacteria; D006923:Hymecromone; D000520:alpha-Glucosidases; D000118:Acetylglucosaminidase; D020559:Monomeric GTP-Binding Proteins