title: Validation of air-displacement plethysmography for estimation of body fat mass in healthy elderly subjects.
abstract: BACKGROUND
  Air-displacement plethysmography (ADP) is a non-invasive method for body composition analysis that divides the body into fat-free mass (FFM) and fat mass (FM) (= 2 compartment model, 2C). It places low demands on subject performance and is therefore most convenient in the elderly.
  
  
  OBJECTIVE
  To validate ADP against dual energy X-ray absorptiometry (DEXA) and to compare it to a four-compartment model of body composition (4C; fat mass, total body water, bone mineral content and residual mass) in the elderly.
  
  
  METHODS
  Body composition was assessed by ADP, DEXA and bioelectrical impedance analysis (BIA) in 26 healthy elderly subjects (15 women, 11 men) aged 60-82 years.
  
  
  RESULTS
  Despite a high correlation of %FM assessed by ADP and DEXA we observed significant differences between the results of these methods for both sexes (2.5 +/-3.4%; bias +/- SD). Deviations of %FM(ADP) from %FM(DEXA) were dependent on bone mineral content (BMC(DEXA)) fraction of FFM. A low BMC(DEXA) was related to an overestimation of DEXA-derived %FM by ADP. There was a systematic bias between results from ADP and the 4C model. 76% of its variance was explained by the assumption of a fixed density of FFM. 96% of the variance in the density of FFM was explained by water content and only 4% by BMC(DEXA) of FFM. When compared to a 4C model, overestimation of %FM(ADP) increases with increasing water fraction of FFM.
  
  
  CONCLUSIONS
  Although there is a tendency for overestimation of %FM(ADP),ADP is a valid method for body composition measurement in the elderly. The bias in %FM(ADP) is mainly related to water content of FFM and indicates that a correction factor for TBW may improve the accuracy of the ADP measurements in the elderly.
authors.affiliation: Institut für Humanernährung & Lebensmittelkunde, Christian-Albrechts-Universität zu Kiel, Düsternbroker Weg 17, 24105, Kiel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bosy-Westphal
authors.forename: M
authors.initials: M
authors.lastname: Mast
authors.forename: C
authors.initials: C
authors.lastname: Eichhorn
authors.forename: C
authors.initials: C
authors.lastname: Becker
authors.forename: D
authors.initials: D
authors.lastname: Kutzner
authors.forename: M
authors.initials: M
authors.lastname: Heller
authors.forename: M J
authors.initials: MJ
authors.lastname: Müller
mesh_terms: D015502:Absorptiometry, Photon; D000273:Adipose Tissue; D000368:Aged; D000369:Aged, 80 and over; D001823:Body Composition / Q000502:physiology*; D001824:Body Constitution; D001834:Body Water / Q000378:metabolism; D015519:Bone Density; D017097:Electric Impedance; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010991:Plethysmography / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity