Determination of itraconazole and hydroxyitraconazole in plasma by use of liquid chromatography-tandem mass spectrometry with on-line solid-phase extraction.
In this paper a method for the simultaneous quantification of the anti-fungal drug itraconazole and its co-active metabolite hydroxyitraconazole in plasma employing liquid chromatography tandem-mass spectrometry and automated solid-phase extraction is described. The method proved rugged, enables short turn-around times and is highly specific. Since there is growing evidence for the importance of therapeutic drug monitoring of itraconazole in the prophylaxis and treatment of invasive fungal infections, the method described here is of interest for a large number of tertiary care hospital laboratories.
Vogeser
Michael
M
Institute of Clinical Chemistry, Hospital of the University of Munich, Munich, Germany. Michael.Vogeser@klch.med.uni-muenchen.de
Spöhrer
Ute
U
Schiel
Xaver
X
D000935:Antifungal Agents / Q000097:blood; D001331:Automation; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; C084794:hydroxyitraconazole; D017964:Itraconazole