A comparative study of Edwardsiella ictaluri parent (EILO) and E. ictaluri rifampicin-mutant (RE-33) isolates using lipopolysaccharides, outer membrane proteins, fatty acids, Biolog, API 20E and genomic analyses.
The biological properties of Edwardsiella ictaluri RE-33 rifampicin-mutant and its parent strain EILO were analysed. RE-33 is an avirulent isolate used as a modified live vaccine against enteric septicaemia of catfish. Electrophoretic analysis of lipopolysaccharide (LPS) patterns showed high homology between both isolates. Further characterization of LPS by immunoblotting revealed the main differences in LPS composition. The RE-33 isolate lacks the high molecular weight bands of LPS (HMW-LPS). Outer membrane protein analysis also showed some immunological differences between RE-33 and the EILO parent strain. Only two fingerprinting techniques, fatty acid composition analysis and Biolog phenotypic profiles, were able to discriminate between both isolates.
Arias
C R
CR
Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA. ariascr@acesag.auburn.edu
Shoemaker
C A
CA
Evans
J J
JJ
Klesius
P H
PH
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D020610:Edwardsiella ictaluri / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D005227:Fatty Acids / Q000032:analysis*; D005393:Fish Diseases / Q000188:drug therapy / Q000382:microbiology; D016680:Genome, Bacterial; D007059:Ictaluridae / Q000382:microbiology; D008070:Lipopolysaccharides / Q000032:analysis*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D012293:Rifampin / Q000494:pharmacology*
D001425:Bacterial Outer Membrane Proteins; D005227:Fatty Acids; D008070:Lipopolysaccharides; D012293:Rifampin