Macromolecular and solution properties of Cepacian: the exopolysaccharide produced by a strain of Burkholderia cepacia isolated from a cystic fibrosis patient.
Light scattering and viscosity measurements were carried out on the previously chemically characterised exopolysaccharide produced by a strain of Burkholderia cepacia isolated from a cystic fibrosis patient. The same exopolysaccharide was also produced by other clinical strains in different laboratories. Therefore, the name Cepacian is now proposed for this exopolysaccharide. Experiments performed as a function of the ionic strength on the native polymer revealed a change in the overall shape of the polymer at low ionic strength. This behaviour was absent in the de-acetylated sample. Potentiometric titrations and light scattering experiments carried out on the acidic form of the native polymer revealed the formation of macromolecular aggregates with a stoichiometry n and 2n stabilised by interactions involving the uronic acid residues.
Sist
Paola
P
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy.
Cescutti
Paola
P
Skerlavaj
Silvia
S
Urbani
Ranieri
R
Leitão
Jorge H
JH
Sá-Correia
Isabel
I
Rizzo
Roberto
R
D000085:Acetates / Q000737:chemistry; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D002704:Chlorates / Q000737:chemistry; D002942:Circular Dichroism; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012542:Scattering, Radiation; D012972:Sodium Hydroxide / Q000737:chemistry; D013696:Temperature; D017186:Titrimetry; D014783:Viscosity
D000085:Acetates; D002704:Chlorates; D011135:Polysaccharides, Bacterial; C487212:cepacian; D012972:Sodium Hydroxide; C032706:sodium chlorate