title: Preparing protein microarrays by soft-landing of mass-selected ions.
abstract: Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures and gently landed at different positions on a surface to form a microarray. An array of cytochrome c, lysozyme, insulin, and apomyoglobin was generated, and the deposited proteins showed electrospray ionization mass spectra that matched those of the authentic compounds. Deposited lysozyme and trypsin retained their biological activity. Multiply charged ions of protein kinase A catalytic subunit and hexokinase were also soft-landed into glycerol-based liquid surfaces. These soft-landed kinases phosphorylated LRRASLG oligopeptide and D-fructose, respectively.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ouyang
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Takáts
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Blake
authors.forename: Bogdan
authors.initials: B
authors.lastname: Gologan
authors.forename: Andy J
authors.initials: AJ
authors.lastname: Guymon
authors.forename: Justin M
authors.initials: JM
authors.lastname: Wiseman
authors.forename: Justin C
authors.initials: JC
authors.lastname: Oliver
authors.forename: V Jo
authors.initials: VJ
authors.lastname: Davisson
authors.forename: R Graham
authors.initials: RG
authors.lastname: Cooks
mesh_terms: D001059:Apoproteins / Q000737:chemistry; D020134:Catalytic Domain; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry / Q000378:metabolism; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000737:chemistry; D007477:Ions; D013058:Mass Spectrometry; D009113:Muramidase / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry; D009496:Neurotensin / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D010766:Phosphorylation; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D007328:Insulin; D007477:Ions; D009211:Myoglobin; D009842:Oligopeptides; D009844:Oligosaccharides; D011506:Proteins; C010857:apomyoglobin; C056529:N-acetylchitohexaose; D009496:Neurotensin; C037642:kemptide; D006593:Hexokinase; D017868:Cyclic AMP-Dependent Protein Kinases; D009113:Muramidase; D014357:Trypsin