title: Bisphenol A suppresses osteoclastic and osteoblastic activities in the cultured scales of goldfish.
abstract: The direct effect of bisphenol A on osteoclasts and osteoblasts was examined using a culture system of goldfish scales. Tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were used as markers of osteoclasts and osteoblasts, respectively. Bisphenol A (10(5) M) significantly suppressed both TRAP and ALP activities. These data were reproduced in an in vivo experiment. From an analysis of a reverse transcription-polymerase chain reaction in the in vitro-cultured scales, it was demonstrated that the insulin-like growth factor (IGF)-1 mRNA expression decreased by a bisphenol A treatment. On the other hand, 17beta-estradiol (E(2)) stimulated both TRAP and ALP activities and did not change IGF-1 mRNA expression, suggesting that bisphenol A has a different effect from E(2) on bone metabolism. This study is the first to demonstrate that bisphenol A functions to suppress directly osteoblasts and osteoclasts among vertebrates, which strongly suggests that this scale in an in vitro assay system can be utilized for the evaluation of the effects of endocrine disrupters on bone cells.
authors.affiliation: Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Uchiura, Ishikawa 927-0553, Japan. nobuo@kenroku.kanazawa-u.ac.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hattori
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001559:Benzhydryl Compounds; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006054:Goldfish; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D034582:Integumentary System; D007527:Isoenzymes / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010010:Osteoclasts / Q000187:drug effects* / Q000201:enzymology; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase; D000098826:Bisphenol A Compounds
chemical_list: D000135:Acid Phosphatase; D000395:Air Pollutants, Occupational; D000469:Alkaline Phosphatase; D001559:Benzhydryl Compounds; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007527:Isoenzymes; D010636:Phenols; D012333:RNA, Messenger; D000071681:Tartrate-Resistant Acid Phosphatase; C006780:bisphenol A; D000098826:Bisphenol A Compounds