title: [The study of orthopoxvirus genes for Kelch-like proteins. II. Construction of cowpox virus variants with targeted gene deletions].
abstract: Integrative plasmids p delta C, p delta D, and p delta G were designed to contain a selective marker beyond the region of homology to virus DNA and to allow construction of recombinant cowpox viruses (CPV) that lack C18L, D11L, or G3L coding for kelch-like proteins. CPV mutants lacking one (C18L, D11L, or G3L), two (D11L/G3L or C18L/D11L), or three (D11L/G3L/C18L, that is, all) kelch-like protein genes of the left variable region of the virus genome were obtained. Impaired reproduction was observed for the triple mutant. Pocks produced by the triple mutant and the original virus differed in size and morphology. In addition, the two CPV variants differed in destructive changes caused in the chorioallantoic membrane of chicken embryos.
authors.affiliation: Institute of Molecular Biology, VECTOR State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 630559 Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Kolosova
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: G V
authors.initials: GV
authors.lastname: Kochneva
authors.forename: E I
authors.initials: EI
authors.lastname: Riabchikova
authors.forename: E V
authors.initials: EV
authors.lastname: Bessmel'tseva
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
authors.forename: T E
authors.initials: TE
authors.lastname: Solenova
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo / Q000821:virology; D002823:Chorion / Q000821:virology; D015605:Cowpox / Q000473:pathology / Q000821:virology; D015608:Cowpox virus / Q000235:genetics* / Q000472:pathogenicity; D017353:Gene Deletion; D009154:Mutation; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D014764:Viral Proteins