title: Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line.
abstract: OBJECTIVE
  To confirm the enhancing effect of excision repair cross complementing rodent repair deficiency gene 2 (ERCC2) on alkylating agents resistance.
  
  
  METHODS
  The authors constructed a pcDNA3-ERCC2 plasmid. The pcDNA3-ERCC2 was transfected into a selected ERCC2 negative human glioma cell line, SKMG-4, using liposome-mediated transfection. After G418 selection, a stable transfected cell line was obtained and tested for cytotoxicity of several alkylating agents.
  
  
  RESULTS
  The stable transfectant was obtained and confirmed by RT-PCR as well as Western blot analysis to be strongly expressing ERCC2 at both mRNA and protein levels. The IC(90) ( micro mol/L) of two alkylating agents, cisplatin and melphalan, increased from 1.0 to 1.75 (75%) and 5.6 to 9.0 (61%), respectively, compared with control cell line.
  
  
  CONCLUSIONS
  The present data provided evidences and confirmed the authors' previous results that ERCC2 contributes, at least partially, to alkylating agent resistance in human glioma cell line.
authors.affiliation: Department of Neurosurgery, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, China. chenzp@gzsums.edu.cn
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.forename: Junying
authors.initials: J
authors.lastname: Zhang
authors.forename: Gérard
authors.initials: G
authors.lastname: Mohr
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005910:Glioma; D006801:Humans; D008558:Melphalan / Q000494:pharmacology; D011506:Proteins / Q000235:genetics*; D014157:Transcription Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D051759:Xeroderma Pigmentosum Group D Protein
chemical_list: D018906:Antineoplastic Agents, Alkylating; D004268:DNA-Binding Proteins; D011506:Proteins; D014157:Transcription Factors; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D002945:Cisplatin; D008558:Melphalan