[An intensification of antiviral and interferon effects of ridostin (an interferon inducer) by cationic liposomes in vitro and in intranasal administration].
Combined application of ridostine with catonic liposomes was shown to essentially enhance the interferon-inducing and antiviral activity of the former in experiments with cell cultures L-929, which is apparently related with an improved efficiency of intracellular delivery of dsRNA. A comparative study demonstrated that ridostine, when combined with liposomes, is needed by 10(3)-10(4) times less as when it is used alone. A pretreatment of the cellular monolayer by cationic liposomes contributes also to enhancing the activity of ridostine, which can be explained by an enhanced permeability of cells for dsRNA holding on-for as long as 30 minutes after the removal of liposomes from the liquid culture. A separate successive administration of, first, liposomes and, then, of ridostine in BALB/c mice (20 mg/kg) leads to a more intensified induction of interferon in the upper respiratory tract tissues as compared with the administration of ridostine alone.
Bulychev
L E
LE
Poryvaev
V D
VD
Ryzhikov
A B
AB
Karpyshev
N N
NN
Alekseeva
A G
AG
Goncharova
E P
EP
Pliasunov
I V
IV
D000281:Administration, Intranasal; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000276:immunology; D018188:Cardiovirus Infections / Q000188:drug therapy* / Q000276:immunology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D016503:Drug Delivery Systems; D004686:Encephalomyocarditis virus / Q000187:drug effects*; D007369:Interferon Inducers / Q000008:administration & dosage / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009831:Olfactory Mucosa / Q000187:drug effects / Q000276:immunology; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000494:pharmacology*; D012331:RNA, Fungal / Q000008:administration & dosage / Q000494:pharmacology*
D000998:Antiviral Agents; D007369:Interferon Inducers; D008081:Liposomes; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; C076741:ridostin; D007372:Interferons