Induction of tyrosine kinase receptor b by retinoic acid allows brain-derived neurotrophic factor-induced amyloid precursor protein gene expression in human SH-SY5Y neuroblastoma cells.
Retinoic acid (RA) is a potent regulator of morphogenesis, growth and cell differentiation. Incubation with RA causes arrest of proliferation and neurite extension in SH-SY5Y cells, a neuroblastoma cell line of human origin. In these cells, RA regulates the expression of the beta-amyloid precursor protein. The retinoid increases the levels of intracellular and secreted forms of APP (amyloid precursor protein), APP-mRNA levels and the activity of the APP promoter in transient transfection studies. These responses require long periods of exposition to the ligand, thus suggesting a nondirect effect of the RA receptors on the APP gene. Also in these cells, RA induces the expression of TrkB, the tyrosine kinase receptor for brain-derived neurotrophic factor (BDNF), and 4 days of pretreatment with retinoic acid confers BDNF responsiveness to the APP promoter.
Ruiz-León
Y
Y
Consejo Superior de Investigaciones, Instituto de Investigaciones Biomédicas Científicas, Arturo Duperier, 4, 28029, Madrid, Spain.
Pascual
A
A
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D001345:Autoradiography; D015152:Blotting, Northern; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014162:Transfection / Q000379:methods; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016564:Amyloid beta-Protein Precursor; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D014316:Tritium; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase; D020794:Receptor Protein-Tyrosine Kinases; D020813:Receptor, trkB; D013936:Thymidine