Detection of dihydrofolate reductase genes by PCR and RFLP.
The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.
Navia
Margarita M
MM
Institut Clínic d'Infeccions i Immunología, IDIBAPS. Hospital Clínic, Facultad de Medicina., Barcelona, Spain.
Ruiz
Joaquim
J
Sanchez-Cespedes
Javier
J
Vila
Jordi
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010597:Pharmacogenetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014296:Trimethoprim Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D013762:Tetrahydrofolate Dehydrogenase