HPLC determination of imidazole antimycotis in antidandruff cosmetic products.
A simple HPLC method for the determination of imidazole antimycotics in cosmetic antidandruff formulations has been developed. HPLC was carried out on a Discovery RP-Amide C16 column and spectrophotometric detection was performed at 220 nm. The initial mobile phase was a mixture of acetonitrile and aqueous 10(-3) M NaClO4 (pH 3.0) in the ratio of 15:85 (v/v); then a linear gradient up to 46% acetonitrile in 70 min, and up to 50% in 80 min. The extraction procedure has been validated by analyzing samples of shampoo and lotion spiked with 1% of the active principles. The recoveries were greater than 95% and the reproducibility was within 3%.
Gagliardi
Luigi
L
Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanitá, Rome, Italy.
De Orsi
Daniela
D
Chimenti
Paola
P
Porra'
Rita
R
Tonelli
Domenica
D
D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results
D000935:Antifungal Agents; D003358:Cosmetics; D007093:Imidazoles; D007202:Indicators and Reagents