title: A newly identified AMSH-family protein is specifically expressed in haploid stages of testicular germ cells.
abstract: Associated Molecule with SH3 domain of STAM (AMSH) plays a critical role in the cytokine-mediated intracellular signal transduction downstream of the Jak2/Jak3-STAM complex. We newly identified a family molecule of AMSH, AMSH-FP (AMSH-Family Protein) in the mouse brain. AMSH-FP encodes the intracellular protein and has a highly conserved JAB1 Subdomain Homologous (JSH) region, suggesting that AMSH-FP may act as adaptor of gene transcription and/or regulation system. AMSH-FP has two splicing forms, one is expressed in various tissues, whereas the other one is restricted to expression in testis. We named the abundant type AMSH-FPalpha and the testis type AMSH-FPbeta. AMSH-FPbeta is a variant lacking N-terminal 166 amino acid residues of AMSH-FPalpha. Analysis of the 5(')-untranslated regions in AMSH-FPalpha and AMSH-FPbeta mRNAs and exon-intron structure of AMSH-FP gene suggests that testis-specific transcripts are generated due to alternative promoter usage and/or alternative splicing. Importantly, AMSH-FPbeta mRNA was not detected in juvenile and infertile mouse testis but was restrictively expressed in the haploid stage of testicular germ cells in the normal mature testis. We suggested that AMSH-FPbeta had a functional role in the spermiogenesis.
authors.affiliation: Division of Molecular Regenerative Medicine, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kitajima
authors.forename: Kunio
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Tahara
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary / Q000378:metabolism; D010450:Endopeptidases; D056827:Endosomal Sorting Complexes Required for Transport; D005091:Exons; D005854:Germ Cells / Q000378:metabolism*; D006238:Haploidy; D017403:In Situ Hybridization; D007438:Introns; D008297:Male; D045726:Metalloproteases; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D014176:Protein Biosynthesis; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology*; D014158:Transcription, Genetic; D043222:Ubiquitin Thiolesterase
chemical_list: D020121:5' Untranslated Regions; D002352:Carrier Proteins; D003062:Codon; D018076:DNA, Complementary; D056827:Endosomal Sorting Complexes Required for Transport; D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger; D010450:Endopeptidases; D045726:Metalloproteases; D010447:Peptide Hydrolases; C441451:Stambp protein, mouse; C477877:Stambpl1 protein, mouse; D043222:Ubiquitin Thiolesterase