title: Instrument-independent flux units for laser Doppler perfusion monitoring assessed in a multi-device study on the renal cortex.
abstract: To investigate the feasibility of instrument-independent perfusion units for laser Doppler flowmetry, a comparison was performed of two commercial fiberoptic laser Doppler perfusion monitors measuring the same flux situation for two different types of probes. In vivo measurements were performed on the cortex of pig's kidney, with an ultrasonic arterial flow meter as reference. The flow was mainly varied by internal arterial constriction using a balloon catheter. For each probe, instruments are compared in terms of the ratio of laser Doppler flux and arterial flow. For a given probe, the flux-to-flow ratios of the two instruments show a linear mutual relationship for a wide variety of arterial flows and laser Doppler fluxes. In vitro measurements were performed on an aqueous suspension of polystyrene microspheres. For the probe with interfiber distance 500 microm the ratio of the in vivo fluxes appears to agree within 16% to the value found in vitro, while for the 250-microm probe a difference of 28% was found. For a wide range of fluxes, the in vivo flux values of one instrument can be translated into flux values for the other instrument, in spite of the instrumental differences. This enables the user to render experimental results independent of the specific instrument, thus facilitating multi-center studies.
authors.affiliation: Faculty of Applied Physics, Biophysical Techniques Group, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: A L
authors.initials: AL
authors.lastname: Petoukhova
authors.forename: W
authors.initials: W
authors.lastname: Steenbergen
authors.forename: F
authors.initials: F
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: Graaff
authors.forename: E D
authors.initials: ED
authors.lastname: de Jong
authors.forename: J M
authors.initials: JM
authors.lastname: Elstrodt
authors.forename: F F M
authors.initials: FF
authors.lastname: de Mul
authors.forename: G
authors.initials: G
authors.lastname: Rakhorst
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D005240:Feasibility Studies; D007672:Kidney Cortex / Q000098:blood supply*; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D008991:Monitoring, Physiologic; D010477:Perfusion; D012016:Reference Values; D012039:Regional Blood Flow; D013552:Swine