title: A highly infective Plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development.
abstract: The New World primate Aotus nancymaae is susceptible to infection by the human malaria parasite Plasmodium vivax and has therefore been recommended by the World Health Organization as a model for malaria vaccine candidate evaluation. We report the isolation, adaptation, titration and genetic characterization of a P. vivax wild strain in splenectomized A. nancymaae monkeys. Parasitemia remained high after 22 passages, reaching 7.88% by Giemsa and Acridine Orange staining and Real-Time PCR determination, making this P. vivax strain a highly infective and reliable asset to be used in P. vivax biological studies and vaccine development.
authors.affiliation: Departamento de Biología Molecular-Malaria, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
authors.forename: Yago
authors.initials: Y
authors.lastname: Pico de Coaña
authors.forename: Josefa
authors.initials: J
authors.lastname: Rodríguez
authors.forename: Eduar
authors.initials: E
authors.lastname: Guerrero
authors.forename: Carlos
authors.initials: C
authors.lastname: Barrero
authors.forename: Raúl
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Marcela
authors.initials: M
authors.lastname: Mendoza
authors.forename: Manuel Alfonso
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D001029:Aotus trivirgatus; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D007621:Karyotyping; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology*; D016780:Malaria, Vivax / Q000097:blood / Q000276:immunology* / Q000469:parasitology*; D008856:Microscopy, Fluorescence; D010966:Plasmodium vivax / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D017780:Malaria Vaccines