Bacillus subtilis diacylglycerol kinase (DgkA) enhances efficient sporulation.
The sn-1,2-diacylglycerol kinase homologue gene, dgkA, is a sporulation gene indispensable for the maintenance of spore stability and viability in Bacillus subtilis. After 6 h of growth in resuspension medium, the endospore morphology of the dgkA mutant by standard phase-contrast microscopy was normal; however, after 9 h, the endospores appeared mostly dark by phase-contrast microscopy, suggesting a defect in the spores. Moreover, electron microscopic studies revealed an abnormal cortex structure in mutant endospores 6 h after the onset of sporulation, an indication of cortex degeneration. In addition, a significant decrease in the dipicolinic acid content of mutant spores was observed. We also found that dgkA is expressed mainly during the vegetative phase. It seems likely that either the DgkA produced during growth prepares the cell for an essential step in sporulation or the enzyme persists into sporulation and performs an essential function.
Amiteye
Samuel
S
International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Kobayashi
Kazuo
K
Imamura
Daisuke
D
Hosoya
Shigeo
S
Ogasawara
Naotake
N
Sato
Tsutomu
T
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D019852:Diacylglycerol Kinase / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008854:Microscopy, Electron; D009154:Mutation; D013171:Spores, Bacterial / Q000502:physiology
D019852:Diacylglycerol Kinase