[Determination of zearalenone and its metabolites in grains by capillary electrophoresis].
OBJECTIVE
To establish a method for the determination of zearalenone and its metabolites in grains by capillary electrophoresis.


METHODS
The samples were extracted with liquid-liquid partition and ultrasonic extraction, cleaned up with C18 cartridges, then determined by micellar electrokinetic capillary chromatography (MEKC).


RESULTS
The limits of detection for Zearalenone (ZON), alpha-Zearalenol (alpha-ZOL), beta-Zearalenol (beta-ZOL), Aflatoxin B1 (AFT B1) and Ochratoxin A (Och A) were 0.0084, 0.081, 0.14, 0.0016 and 0.031 microgram/ml, respectively. Recoveries of all compounds were in the range of 77.9%-103.1%. Relative standard deviations (RSDs) were 0.63%-1.98%.


CONCLUSIONS
This sensitive and accurate method can be used in the determination of zearalenone and its metabolites in grains.
Zeng
Hongyan
H
Department of Sanitary Technology, West China School of Pulic Health, Sichuan University, Chengdu 610041, China.
Li
Yuanqian
Y
Jin
Jun
J
Sun
Huiqin
H
D016604:Aflatoxin B1 / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary; D002523:Edible Grain / Q000382:microbiology*; D019075:Electrophoresis, Capillary; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D009183:Mycotoxins / Q000032:analysis; D009793:Ochratoxins / Q000032:analysis; D015025:Zearalenone / Q000032:analysis* / Q000378:metabolism*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis*
D009183:Mycotoxins; D009793:Ochratoxins; C029659:zearalenol; C025589:ochratoxin A; D015025:Zearalenone; D015029:Zeranol; D016604:Aflatoxin B1