title: Immobilised enzymes: carrier-bound or carrier-free?
abstract: Recent advances have been made in the development of carrier-free immobilised enzymes and several criteria established for the selection of immobilised enzymes for biotransformations. The pros and cons of carrier-free versus carrier-bound immobilised enzymes and of each type of carrier-free enzyme are discussed.
authors.affiliation: Laboratory of Biocatalysis and Organic Chemistry, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands.
authors.forename: Linqiu
authors.initials: L
authors.lastname: Cao
authors.forename: Luuk van
authors.initials: Lv
authors.lastname: Langen
authors.forename: Roger A
authors.initials: RA
authors.lastname: Sheldon
mesh_terms: D002384:Catalysis; D020134:Catalytic Domain; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000138:chemical synthesis / Q000737:chemistry* / Q000191:economics; D007700:Kinetics; D008863:Microspheres; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D012995:Solubility; D012997:Solvents; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011108:Polymers; D012997:Solvents