[Cloning and sequence analysis of the molecular-adjuvant gene of the complement C3d of mouse].
OBJECTIVE
Considering that the complement C3d plays a stronger positive role in the control of immunologic system, we constructed the recombinant plasmid of the molecular-adjuvant gene of the complement C3d in order to enhance the immunologic efficacy of the gene vaccine.


METHODS
The main points were amplifying the segment of complement C3d gene by using of PCR; inserting it into PGEM-T; detecting it by digesting with Eco RI and sequencing; the whole length being 897 bp; then sub-cloning it into pVAX1.


RESULTS
The DNA sequencing confirmed the C3d sequence.


CONCLUSIONS
We have successfully cloned the gene of C3d and constructed the recombinant plasmid of pVAX1 C3d.
Sun
Meiqin
M
Department of Infecious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhao
Liansan
L
Chen
Shouchun
S
Wang
Song
S
Zhou
Taoyou
T
Tang
Hong
H
Chen
Yirong
Y
Yan
Juan
J
D000818:Animals; D003001:Cloning, Molecular; D015933:Complement C3d / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D017422:Sequence Analysis, DNA; D019444:Vaccines, DNA / Q000276:immunology
D019444:Vaccines, DNA; D015933:Complement C3d