Cloning and identification of the human LPAAT-zeta gene, a novel member of the lysophosphatidic acid acyltransferase family.
Lysophosphatidic acid (LPA) is a naturally occurring component of phospholipid and plays a critical role in the regulation of many physiological and pathophysiological processes including cell growth, survival, and pro-angiogenesis. LPA is converted to phosphatidic acid by the action of lysophosphatidic acid acyltransferase (LPAAT). Five members of the LPAAT gene family have been detected in humans to date. Here, we report the identification of a novel LPAAT member, which is designated as LPAAT-zeta. LPAAT-zeta was predicted to encode a protein consisting of 456 amino acid residues with a signal peptide sequence and the acyltransferase domain. Northern blot analysis showed that LPAAT-zeta was ubiquitously expressed in all 16 human tissues examined, with levels in the skeletal muscle, heart, and testis being relatively high and in the lung being relatively low. The human LPAAT-zeta gene consisted of 13 exons and is positioned at chromosome 8p11.21.
Li
Dan
D
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Yu
Long
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China. longyu@fudan.edu.cn.|Institute of Genetics, Fudan University, 220 Handan Road, 200433, Shanghai, P.R. China. longyu@fudan.edu.cn.
Wu
Hai
H
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Shan
Yuxi
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Guo
Jinhu
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Dang
Yongjun
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Wei
Youheng
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
Zhao
Shouyuan
S
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase