title: Antigenic and genetic variability of human respiratory syncytial viruses (group A) isolated in Uruguay and Argentina: 1993-2001.
abstract: The antigenic and genetic diversity of G glycoprotein from 25 human respiratory viruses (group A) isolated during nine consecutive epidemics (1993-2001) in Montevideo, Uruguay, and 7 strains isolated in Buenos Aires, Argentina, in the same period were analyzed. Genetic variability was evaluated by partial sequence of the G protein gene. Phylogenetic analysis indicated that most Uruguayan and Argentinean group A isolates clustered into three genotypes: GA5, GA2, and GA1. Some strains clustered into the GA3 genotype characterized previously. The antigenic analysis was carried out with a panel of anti-G monoclonal antibodies that recognized conserved and strain-specific epitopes. A close correlation between the antigenic and genetic relatedness of the strains analyzed was observed.
authors.affiliation: Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
authors.forename: Sandra
authors.initials: S
authors.lastname: Frabasile
authors.forename: Adriana
authors.initials: A
authors.lastname: Delfraro
authors.forename: Luján
authors.initials: L
authors.lastname: Facal
authors.forename: Cristina
authors.initials: C
authors.lastname: Videla
authors.forename: Mónica
authors.initials: M
authors.lastname: Galiano
authors.forename: María José
authors.initials: MJ
authors.lastname: de Sierra
authors.forename: Dora
authors.initials: D
authors.lastname: Ruchansky
authors.forename: Nathalia
authors.initials: N
authors.lastname: Vitureira
authors.forename: Mabel
authors.initials: M
authors.lastname: Berois
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Carballal
authors.forename: José
authors.initials: J
authors.lastname: Russi
authors.forename: Juan
authors.initials: J
authors.lastname: Arbiza
mesh_terms: D000595:Amino Acid Sequence; D000940:Antigenic Variation; D000956:Antigens, Viral; D001118:Argentina / Q000453:epidemiology; D002675:Child, Preschool; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014583:Uruguay / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D014764:Viral Proteins