Structure of the lipopolysaccharide of Pseudomonas aeruginosa O-12 with a randomly O-acetylated core region.
The lipopolysaccharide of Pseudomonas aeruginosa O-12 was studied by strong alkaline and mild acid degradations and dephosphorylation followed by fractionation of the products by GPC and high-performance anion-exchange chromatography and analyses by ESI FT-MS and NMR spectroscopy. The structures of the lipopolysaccharide core and the O-polysaccharide repeating unit were elucidated and the site and the configuration of the linkage between the O-polysaccharide and the core established. The core was found to be randomly O-acetylated, most O-acetyl groups being located on the terminal rhamnose residue of the outer core region.
Bystrova
Olga V
OV
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, 119991 Moscow, Russia.
Lindner
Buko
B
Moll
Herman
H
Kocharova
Nina A
NA
Knirel
Yuriy A
YA
Zähringer
Ulrich
U
Pier
Gerald B
GB
D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003837:Deoxy Sugars / Q000032:analysis; D004731:Endotoxins / Q000737:chemistry*; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D007536:Isomerism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry*; D025942:Oligosaccharides, Branched-Chain / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013400:Sugar Acids / Q000032:analysis
D003837:Deoxy Sugars; D004731:Endotoxins; D006539:Heptoses; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; C095976:O antigen, Pseudomonas; D025942:Oligosaccharides, Branched-Chain; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C042058:core endotoxin; C048215:2-fucosamine; D005643:Fucose; C026312:glycero-manno-heptose; C120356:3-deoxy-manno-oct-2-ulopyranosonic acid