Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch.
Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.
Huang
Ying
Y
Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, People's Republic of China.
Huang
Qichen
Q
Chen
Haibao
H
Tang
Youqi
Y
Miyake
Hideo
H
Kusunoki
Masami
M
D000818:Animals; D003460:Crystallization / Q000379:methods*; D011994:Recombinant Proteins; D012604:Scorpion Venoms / Q000737:chemistry*; D014961:X-Ray Diffraction
C485978:BmK alphaIT1 toxin, Buthus martensii; D011994:Recombinant Proteins; D012604:Scorpion Venoms