title: A single nucleotide polymorphism in the E-cadherin gene promoter-160 is not associated with risk of Korean gastric cancer.
abstract: Recently, the -160 C/A polymorphism, located within the regulatory region of Ecadherin promoter, has been shown to influence E-cadherin transcription by altering transcription factor binding. We examined the effect of this polymorphism on risk of gastric cancer and on histological classification of intestinal- and diffuse-type gastric cancer in 146 normal healthy individuals and 292 Korean gastric cancer patients. Genomic DNA samples were examined by polymerase chain reaction (PCR)-single strand conformational polymorphism (SSCP)-sequencing and confirmed by restriction fragment length polymorphism (RFLP). Unexpectedly, there was no significant difference in the genotype frequencies of the polymorphism between normal control and gastric cancer patients (x(2) test, p=0.433). The estimated odd ratio of C/C to A/A genotype in gastric cancer cases was 1.07 (95% confidence interval, 0.396-2.870). We also found no evidence for differences in risk for the intestinal- and diffuse-type gastric cancer. These results suggest that the -160 C/A polymorphism of the E-cadherin has no direct effect on the risk of Korean gastric cancer development and on its histological classification.
authors.affiliation: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.forename: Yong Gu
authors.initials: YG
authors.lastname: Cho
authors.forename: Jik Young
authors.initials: JY
authors.lastname: Park
authors.forename: Chang Jae
authors.initials: CJ
authors.lastname: Kim
authors.forename: Jong Heun
authors.initials: JH
authors.lastname: Lee
authors.forename: Hong Sug
authors.initials: HS
authors.lastname: Kim
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Young Hwa
authors.initials: YH
authors.lastname: Song
authors.forename: Cho Hyun
authors.initials: CH
authors.lastname: Park
authors.forename: Yong Kyu
authors.initials: YK
authors.lastname: Park
authors.forename: Su Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
authors.forename: Sug Hyung
authors.initials: SH
authors.lastname: Lee
authors.forename: Nam Jin
authors.initials: NJ
authors.lastname: Yoo
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000483:Alleles; D015820:Cadherins / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007723:Korea; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D012306:Risk; D013274:Stomach Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015820:Cadherins; D004247:DNA