Multiple dendritic cell (DC) subpopulations in human gingiva and association of mature DCs with CD4+ T-cells in situ.
Gingival epithelium is a site of active trafficking of Langerhans cells (LCs), while the lamina propria in chronic periodontitis (CP) contains CD83+ mature dendritic cells (mDCs) and CD4+ T-cells. The immune cells that contribute to the mDCs, and whether mDCs engage with T-cells in situ, are unclear. Using several immunohistochemical approaches, combined with fluorescence-, light-, and scanning laser confocal-microscopy, we show that, in addition to LCs, the gingiva contains dermal DCs (DDCs) in the lamina propria; moreover, DDCs increase in number during CP. Furthermore, DDCs, LCs, and B-cells co-express CD83 in CP and contribute to the mDC pool. Double-staining for CD83 and CD4 revealed that mDCs associate with clusters of CD4+ T-cells in the lamina propria. Analysis of these data suggests that multiple DC subsets mature in the gingiva and that mature DCs engage in antigen presentation with T-cells in chronic periodontitis.
Jotwani
R
R
Department of Periodontics, 110 Rockland Hall, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-8703, USA.
Cutler
C W
CW
D000328:Adult; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000145:classification*; D002452:Cell Count; D002908:Chronic Disease; D003713:Dendritic Cells / Q000145:classification*; D004848:Epithelium / Q000276:immunology; D019085:Fluorescent Antibody Technique, Direct; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D018655:Lymphocyte Count; D008562:Membrane Glycoproteins / Q000032:analysis; D018613:Microscopy, Confocal; D010518:Periodontitis / Q000276:immunology; D000097802:CD83 Antigen
D015703:Antigens, CD; D015704:CD4 Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen