title: Usefulness of deoxyribonuclease I (DNase I) polymorphism for individualization from small aged urine stains.
abstract: We devised a procedure that combines a simple extraction method, isoelectric focusing and activity staining using the dried agarose film overlay method, for deoxyribonuclease I (DNase I) typing from aged urine stains. DNase I types were determined without difficulty from urine stains kept at room temperature for 3 months or more in all of the samples tested. The amounts of urine stains required for typing after 3 months of storage were estimated to be equivalent to 60-120 microl of liquid urine. Therefore, considering that useful PCR-based DNA typing has not yet been developed for urine stains, DNase I polymorphism could be considered the first biochemical marker found to be well suited for individualization from small aged urine stains.
authors.affiliation: Department of Biology, Fukui Medical University, 910-1193, Fukui, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
authors.forename: Misuzu
authors.initials: M
authors.lastname: Ueki
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.forename: Koichi
authors.initials: K
authors.lastname: Mogi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Reiko
authors.initials: R
authors.lastname: Iida
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kishi
mesh_terms: D002847:Chromatography, Agarose; D016172:DNA Fingerprinting / Q000379:methods; D003850:Deoxyribonuclease I / Q000235:genetics* / Q000652:urine*; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013997:Time Factors
chemical_list: D005819:Genetic Markers; D003850:Deoxyribonuclease I