Characteristics of apoptotic cell death induced by coxsackievirus B in permissive Vero cells.
Coxsackievirus B (CVB) causes a wide spectrum of human diseases which are closely associated with direct destruction of infected cells. We investigated the morphological and biochemical characteristics of CPEs in permissive Vero cells caused by different CVB serotypes. Regardless of serotype, the infected cells experienced similar degrees of CPEs within 24 h postinfection (p.i.). Using both Hoechst 33342 staining and transmission electron microscopy, we consistently observed morphological properties of apoptosis, heavily condensed nuclei and subsequent chromatin condensation into the periphery of the nuclei within 12 h p.i. Moreover, we noticed typical oligonucleosomal DNA fragmentation, while productive CVB multiplication was accomplished within 6 h p.i. prior to an apoptotic signal. Caspase inhibitor significantly prohibited nuclear changes due to apoptosis with no influence on virus production and cell death, demonstrating that all the CVBs induced more than one type of pathological effect, including apoptotic alteration in permissive Vero cells.
Ahn
Jeonghyun
J
Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea.
Joo
Chul-Hyun
CH
Seo
Ilsun
I
Kim
DongHou
D
Hong
Hea Nam
HN
Kim
Yoo Kyum
YK
Lee
Heuiran
H
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001562:Benzimidazoles; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000145:classification / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D012703:Serotyping; D013194:Staining and Labeling; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication
D001562:Benzimidazoles; C017807:bisbenzimide ethoxide trihydrochloride