title: Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping.
abstract: The radish Rfo gene restores male fertility in radish or rapeseed plants carrying Ogura cytoplasmic male-sterility. This system was first discovered in radish and was transferred to rapeseed for the production of F1 hybrid seeds. We aimed to identify the region of the Arabidopsis genome syntenic to the Rfo locus and to characterize the radish introgression in restored rapeseed. We used two methods: amplified consensus genetic markers (ACGMs) in restored rapeseed plants and construction of a precise genetic map around the Rfo gene in a segregating radish population. The use of ACGMs made it possible to detect radish orthologs of Arabidopsis genes in the restored rapeseed genome. We identified radish genes, linked to Rfo in rapeseed and whose orthologs in Arabidopsis are carried by chromosomes 1, 4 and 5. This indicates several breaks in colinearity between radish and Arabidopsis genomes in this region. We determined the positions of markers relative to each other and to the Rfo gene, using the progeny of a rapeseed plant with unstable meiotic transmission of the radish introgression. This enabled us to produce a schematic diagram of the radish introgression in rapeseed. Markers which could be mapped both on radish and restored rapeseed indicate that at least 50 cM of the radish genome is integrated in restored rapeseed. Using markers closely linked to the Rfo gene in rapeseed and radish, we identified a contig spanning six bacterial artificial chromosome (BAC) clones on Arabidopsis chromosome 1, which is likely to carry the orthologous Rfo gene.
authors.affiliation: Station de Génétique et d'Amélioration des Plantes, INRA, Route de Saint-Cyr, 78026, Versailles cedex, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Giancola
authors.forename: Sylvie
authors.initials: S
authors.lastname: Marhadour
authors.forename: Sophie
authors.initials: S
authors.lastname: Desloire
authors.forename: Vanessa
authors.initials: V
authors.lastname: Clouet
authors.forename: Hélène
authors.initials: H
authors.lastname: Falentin-Guyomarc'h
authors.forename: Wassila
authors.initials: W
authors.lastname: Laloui
authors.forename: Cyril
authors.initials: C
authors.lastname: Falentin
authors.forename: Georges
authors.initials: G
authors.lastname: Pelletier
authors.forename: Michel
authors.initials: M
authors.lastname: Renard
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
authors.forename: Régine
authors.initials: R
authors.lastname: Delourme
authors.forename: Françoise
authors.initials: F
authors.lastname: Budar
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D001937:Brassica / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D018745:Genome, Plant; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins