Influence of genetic polymorphisms of CYP1A1 and GSTM1 on the urinary levels of 1-hydroxypyrene.
The aim of the present study is to evaluate the influence of the genetic polymorphism of two enzymes involved in the biotransformation of xenobiotics, cytochrome P450 1A1 (CYP1A1) and glutathione-S-transferase M1 (GSTM1), on the urinary levels of 1-hydroxypyrene (1-OH-P) in workers exposed to polycyclic aromatic hydrocarbons (PAHs) and in unexposed workers (controls). The study group consisted of 30 controls recruited among employees of a service company and 171 PAHs-exposed workers from two electric steel plants and an iron foundry (all males, ranging between 18 and 60 years of age). Determination of airborne PAHs and urinary 1-OH-P was performed by high-performance liquid chromatography (HPLC) with fluorimetric detection. Polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) was used to determine the genetic polymorphisms of CYP1A1 (CYP1A1*2A and CYP1A1*2B) and GSTM1. No influence of the genetic polymorphism of CYP1A1 and GSTM1 on the urinary levels of 1-OH-P was observed in this study.
Apostoli
P
P
Chair of Industrial Hygiene, Department of Experimental and Applied Medicine, University of Brescia, P.le Spedali Civili, 1, Brescia 25123, Italy. apostoli@med.unibs.it
Neri
G
G
Lucas
D
D
Manno
M
M
Berthou
F
F
D000293:Adolescent; D000328:Adult; D000397:Air Pollution / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D004784:Environmental Monitoring; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008669:Metallurgy; D008875:Middle Aged; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011110:Polymorphism, Genetic / Q000235:genetics*; D011721:Pyrenes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C033146:1-hydroxypyrene