Structure and oligomeric state of the mammalian tumour-associated antigen UK114.
The tumour-associated antigen UK114, isolated from goat liver, belongs to the YER057c/YIL051c/YjgF protein family, which has members in both the prokaryotes and eukaryotes. The crystal structure of a mammalian representative, goat UK114, was determined, revealing a trimeric arrangement in the crystal. It was confirmed by ultracentrifugation that UK114 is a trimer in solution. These results are in agreement with the published structures of homologues from unicellular organisms, but contrast with those reported for the rat homologue of UK114, for which a dimeric quaternary structure was proposed.
Deriu
Daniela
D
Institute of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Briand
Christophe
C
Mistiniene
Edita
E
Naktinis
Vytautas
V
Grütter
Markus G
MG
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization; D018360:Crystallography, X-Ray; D006041:Goats; D008099:Liver / Q000737:chemistry; D008958:Models, Molecular; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D012996:Solutions; D014461:Ultracentrifugation
D009363:Neoplasm Proteins; D012996:Solutions; C101630:UK114 antigen