[Eggs and reproductive organs of female Angiostrongylus costaricensis are more intensely recognized by human sera from acute phase in abdominal angiostrongyliasis].
Angiostrongylus costaricensisis is an intra-arterial nematode of rodents. Man may become accidentally infected through ingestion of contaminated food or water. Our objective was to describe the parasite structures recognized by human antibodies in serum samples from acute and convalescent phases of abdominal angiostrongyliasis. An indirect immunofluorescent method was employed to study reactivity on whole eggs and sections of female worms and first stage larvae (L1). L1 were also studied before and after sonication. Fluorescence, always higher with acute phase sera, was detected on the surface of whole eggs and in L1 fragments and was neither present on whole L1 nor on their sections. An inespecific reactivity was seen on the cuticular border of the general cavity and reproductive organs. The data indicate the latter as a main source of antigenicity.
Bender
Ana Lígia
AL
Laboratório de Parasitologia Molecular, Instituto de Pesquisas Biomédicas, Pontificia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Maurer
Rafael Lucyk
RL
da Silva
Márcia Cristina Fernandes
MC
Ben
Renata
R
Terraciano
Paula Barros
PB
da Silva
Ana Cristina Aramburu
AC
Graeff-Teixeira
Carlos
C
D000208:Acute Disease; D000801:Angiostrongylus / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010057:Oviducts / Q000276:immunology*; D010063:Ovum / Q000276:immunology*; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000276:immunology*
D000947:Antigens, Helminth