[RNA, unlike DNA, binds to aurintricarboxylic acid via covalent bonds: molecular-biological and spectroscopic evidence].
A selective interaction of ATA with RNA, unlike DNA, when isolating nucleic acids from plant materials was established. The data concerning the binding strength of highly purified RNAATA and DNAATA preparations to nitrocellulose and nylon filters under conditions of high ionic strength are presented. The interrelation of ATA RNA-tropism and absence of adsorption capability of chemical modified RNAATA the backbone was observed. Using IR spectroscopy under the procedure of multi-broken complete light reflection, a formation of ATA and RNA complex was fixed via phosphoric-ether bond (P-O-C), which was absent in the case of DNAATA. The obtained data raise the problem of RNAATA application in molecular biology experiments.
Martynenko
E I
EI
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv. Martynenko_Olena@ukr.net
Negrebetskaia
E N
EN
Stepaniugin
A V
AV
Samoĭlenko
S A
SA
Govorun
D N
DN
D001312:Aurintricarboxylic Acid / Q000378:metabolism*; D003101:Collodion / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification*; D031962:Kalanchoe / Q000737:chemistry; D009757:Nylons / Q000378:metabolism; D009994:Osmolar Concentration; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D018749:RNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods*
D009757:Nylons; D018749:RNA, Plant; D001312:Aurintricarboxylic Acid; D012313:RNA; D003101:Collodion; D004247:DNA