[Effects of epithelial growth factor receptor inhibitor on the growth and repair of airway epithelial cells].
OBJECTIVE
To explore the possible effects of epithelial growth factor receptor inhibitor on the growth and repair of airway epithelial cells.


METHODS
MTT assay was used to measure the effects of several concentrations of epithelial growth factor receptor inhibitor Tyrphostin AG1478 on the cell proliferation of rat tracheal epithelial cells in primary culture. And 24 hours after scraping to off a certain amount of cells, we measured the changes of scraped area in two groups of cell culture exposed to Tyrphostin AG 1478 at the concentration of 2.5 mumol/L and low-serum culture medium.


RESULTS
Tyrphostin AG 1478 inhibited the proliferation of tracheal epithelial cells depending on concentrations. In the meantime, the scraped area of Tyrphostin AG 1478 group was much larger than that of low-serum group, P < 0.001.


CONCLUSIONS
Epithelial growth factor receptor inhibitor Tyrphostin AG1478 has an inhibitory effect on the growth of rat tracheal epithelial cells and it may prevent the repair of airway epithelium.
Long
Huaicong
H
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China.
Wang
Zengli
Z
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000378:metabolism; D066246:ErbB Receptors / Q000037:antagonists & inhibitors*; D008297:Male; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000166:cytology*; D020032:Tyrphostins / Q000494:pharmacology*
D016228:Endothelial Growth Factors; D020032:Tyrphostins; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases