IL-17 enhances the susceptibility of U-2 OS osteosarcoma cells to NK cell lysis.
We investigated the effect of the proinflammatory cytokine interleukin 17 (IL-17) on the lysis of osteosarcoma cells by human NK cells. NK cells and U-2 OS, MG-63, HOS osteosarcoma cell lines express the IL-17 receptor, the highest amount being found on U-2 OS. Pre-incubation of NK cells with IL-17 did not affect the cytotoxicity against osteosarcomas, that was increased when U-2 OS were pre-incubated with IL-17. In IL-17 treated U-2 OS osteosarcoma cells FACS analysis demonstrated an increased expression of fibronectin among the panel of adhesion molecules assayed, and the treatment with anti-fibronectin antibodies decreased the NK cytotoxicity. The comparison between interferon gamma (IFN-gamma) treated and IFN-gamma/IL-17-treated U-2 OS showed a decreased susceptibility to NK lysis associated with a reduced expression of CD49f on U-2 OS treated with IFN-gamma/IL-17. IL-17 appears to be a modulator of NK adhesion molecules on U-2 OS cells but antagonizes with IFN-gamma on NK lysis.
Honorati
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MC
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy. labimge@alma.unibo.it
Neri
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S
Cattini
L
L
Facchini
A
A
D000328:Adult; D001859:Bone Neoplasms / Q000276:immunology* / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D039421:Integrin alpha2 / Q000378:metabolism; D039503:Integrin alpha6 / Q000378:metabolism; D007371:Interferon-gamma / Q000627:therapeutic use; D020381:Interleukin-17 / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000276:immunology*; D012516:Osteosarcoma / Q000276:immunology* / Q000378:metabolism; D018123:Receptors, Interleukin / Q000032:analysis; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins / Q000032:analysis; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D005353:Fibronectins; C109985:IL17RA protein, human; D039421:Integrin alpha2; D039503:Integrin alpha6; D020381:Interleukin-17; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins; D007371:Interferon-gamma