title: Multiplex short tandem repeat typing in degraded samples using newly designed primers for the TH01, TPOX, CSF1PO, and vWA loci.
abstract: We performed multiplex polymerase chain reaction (PCR) for the TH01, TPOX, CSF1PO, and vWA loci using a newly designed pair of primers that yield smaller fragments than reported previously [Fujii et al., J Hum Genet 45 (2000) 303; Lederer et al., Int J Legal Med 114 (2000) 87]. These loci can be detected in the range of 74-143 bp amplifying products. This system required genomic DNA in a range of 80 pg to 2 ng, and proved to be a sensitive typing method. We compared our system against the GenePrint Fluorescent STR Multiplex Systems CTTv (Promega, Madison, WI, USA), using DNA extracted from old bloodstains left to stand for 17-26 years at room temperature. With our designed system, all allele-typing efforts were successful in the range of 1-5 ng DNA, while no signal peaks were detected, even with when using 10 ng of DNA GenePrint Fluorescent STR Multiplex Systems CTTv.
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano, Japan. tuk-lab@mx1.avis.ne.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukada
authors.forename: Kayoko
authors.initials: K
authors.lastname: Takayanagi
authors.forename: Hideki
authors.initials: H
authors.lastname: Asamura
authors.forename: Masao
authors.initials: M
authors.lastname: Ota
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Fukushima