Excessive production of nitric oxide induces the neuronal cell death in lipopolysaccharide-treated rat hippocampal slice culture.
In this study, we investigated the role of nitric oxide (NO) in neuronal cell death in the lipopolysaccharide (LPS)-treated rat hippocampal slice culture. Neuronal cell death was induced after a LPS treatment in a rat hippocampal slice. The region and time course of the cell death caused by LPS were different from those by N-methyl-D-aspartate. Cell death was inhibited when the slice was co-treated with both LPS and N-monomethyl-L-arginine, a nitric oxide synthase inhibitor. On the other hand, sodium nitroprusside, a NO donor, induced cell death significantly. A reverse transcription-polymerase chain reaction and Western analysis showed that inducible nitric oxide synthase (iNOS) was induced in the LPS-treated hippocampal slice culture. These results suggest that NO produced by iNOS is a major mediator of cell death in LPS-treated hippocampal slice culture.
Lee
Pyeongjae
P
Department of Herbal Pharmacology, Graduate School of East-West Medical Science, Kyunghee University, #1 Hoigi-dong, Dongdaemun-gu, Seoul 130-701, South Korea.
Son
Dongwook
D
Lee
Jongseok
J
Kim
Yong Sik
YS
Kim
Hocheol
H
Kim
Sun Yeou
SY
D000818:Animals; D000831:Animals, Newborn; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D016923:Cell Death / Q000502:physiology*; D002544:Cerebral Infarction / Q000378:metabolism* / Q000503:physiopathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009410:Nerve Degeneration / Q000378:metabolism* / Q000503:physiopathology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D020030:Nitric Oxide Donors; D012333:RNA, Messenger; D009569:Nitric Oxide; D016202:N-Methylaspartate; D019001:Nitric Oxide Synthase