title: Twin pregnancy obtained with frozen-thawed embryos after in vitro maturation in a patient with polycystic ovarian syndrome.
abstract: OBJECTIVE
  A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed.
  
  
  METHODS
  The patient had received mild hMG stimulation followed by hCG injection. After culture for 24-48 h, mature oocytes were fertilized by ICSI. Embryos were cultured until day 3; supernumerary embryos were cryopreserved using a slow protocol.
  
  
  RESULTS
  Among 15 nonatretic oocytes, 9 matured, 8 were fertilized. Four embryos were transferred but they did not implant. The subsequent transfer of three frozen-thawed embryos resulted in the delivery of two healthy girls.
  
  
  CONCLUSIONS
  These results indicate that a pregnancy could be obtained with in vitro maturation-derived day-3 frozen-thawed embryos.
authors.affiliation: Gynecology-Obstetrics Department, Citadelle Hospital, Liège, Belgium.
authors.forename: P-A
authors.initials: PA
authors.lastname: Godin
authors.forename: O
authors.initials: O
authors.lastname: Gaspard
authors.forename: F
authors.initials: F
authors.lastname: Thonon
authors.forename: C
authors.initials: C
authors.lastname: Jouan
authors.forename: F
authors.initials: F
authors.lastname: Wijzen
authors.forename: M
authors.initials: M
authors.lastname: Dubois
authors.forename: J-M
authors.initials: JM
authors.lastname: Foidart
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D011085:Polycystic Ovary Syndrome; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D014427:Twins