Expression of adhesion molecules by cultured spiral ligament fibrocytes stimulated with proinflammatory cytokines.
Secondary cultures from murine spiral ligament (SL) fibrocytes were stimulated with proinflammatory cytokines interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), and expression of various adhesion molecules was investigated. Cultures without cytokine stimulation did not show positive immunostaining for vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), or mucosal addressin cell adhesion molecule-1 (MAdCAM-1). Although staining was also negative after stimulation with IL-1beta, VCAM-1 and ICAM-1 staining was observed after the cells were stimulated with TNF-alpha. Reverse transcription-polymerase chain reaction analysis showed messenger RNAs for both VCAM-1 and ICAM-1 expression to be present after fibrocytes were stimulated with TNF-alpha. These data suggest that activated fibrocytes may cause inflammatory cells to persist in the SL. Given that SL fibrocytes may play a role in homeostasis of cochlear fluid and ion concentrations, prolongation of the inflammatory response could lead to fibrocyte damage that might ultimately result in cochlear malfunction.
Ichimiya
Issei
I
Department of Immunology and Allergy (Otolaryngology), Oita Medical University, Oita, Japan.
Yoshida
Kazuhide
K
Suzuki
Masashi
M
Mogi
Goro
G
D000818:Animals; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured / Q000187:drug effects; D003051:Cochlea / Q000166:cytology / Q000187:drug effects*; D007136:Immunoglobulins / Q000187:drug effects; D018836:Inflammation Mediators / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D007375:Interleukin-1 / Q000494:pharmacology*; D008022:Ligaments / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009088:Mucoproteins / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics
D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D018836:Inflammation Mediators; D007375:Interleukin-1; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1