title: [Determination of peroxidase positive cells in semen: is it a secure parameter for the diagnosis of silent genital infections?].
abstract: BACKGROUND
  The presence of leukocytes, detected by peroxidase test in semen, can be a good indicator of infections in the male genital tract. Peroxidase positive cells have been positively correlated with elevated values of elastase, one of the major proteases liberated by granulocytes at the inflammation place. However, seminal granulocytes may not be adequately detected by the peroxidase test in comparison with immunological methods.
  
  
  OBJECTIVE
  To correlate the determination of peroxidase positive cells with the elastase level in the seminal plasma.
  
  
  METHODS
  Seminal plasma from 64 patients with a high number of round cells (> 106/ml) in semen, was studied. Correlation analysis was done using the Pearson correlation coefficient.
  
  
  RESULTS
  No correlation between the level of granulocyte elastase and the number of peroxidase positive cells (r = 0.2237, p > 0.05), or even the number of round cells (r = 0.03934, p > 0.05) was observed.
  
  
  CONCLUSIONS
  Our results suggest that the determination of peroxidase positive cells is not a reliable indicator of leukocytes in the seminal plasma and their absence do not discard a silent genital tract infection.
authors.affiliation: Centro de Biotecnología en Reproducción, Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile. rsanchez@ufro.cl
authors.forename: Raúl
authors.initials: R
authors.lastname: Sánchez
authors.forename: Juana
authors.initials: J
authors.lastname: Villegas
authors.forename: Patricio
authors.initials: P
authors.lastname: Peña
authors.forename: Werner
authors.initials: W
authors.lastname: Miska
authors.forename: Wolf-Bernhard
authors.initials: WB
authors.lastname: Schill
mesh_terms: D015415:Biomarkers / Q000032:analysis; D004796:Clinical Enzyme Tests / Q000379:methods*; D005832:Genital Diseases, Male / Q000175:diagnosis*; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007239:Infections / Q000175:diagnosis*; D019272:Leukocyte Elastase / Q000032:analysis*; D007962:Leukocytes / Q000201:enzymology; D008297:Male; D009195:Peroxidase / Q000032:analysis*; D015203:Reproducibility of Results; D012661:Semen / Q000166:cytology / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D009195:Peroxidase; D019272:Leukocyte Elastase