Glomerular territories in the olfactory bulb from the larval stage of the sea lamprey Petromyzon marinus.
The goal of this study was to investigate the spatial organization of olfactory glomeruli and of substances relevant to olfactory sensory neuron activity in the developing agnathan, the sea lamprey Petromyzon marinus. A 45-kD protein immunoreactive to G(olf), a cAMP-dependent olfactory G protein, was present in the ciliary fraction of sea lamprey olfactory epithelium and in olfactory sensory neurons of larval and adult sea lampreys. This result implies that G(olf) expression was present during early vertebrate evolution or evolved in parallel in gnathostome and agnathostome vertebrates. Serial sectioning of the olfactory bulb revealed a consistent pattern of olfactory glomeruli stained by GS1B(4) lectin and by anterograde labeling with fluorescent dextran. These glomerular territories included the dorsal cluster, dorsal ring, anterior plexus, lateral chain, medial glomeruli, ventral ring, and ventral cluster. The dorsal, anterior, lateral, and ventral glomeruli contained olfactory sensory axon terminals that were G(olf)-immunoreactive. However, a specific subset, the medial glomeruli, did not display this immunoreactivity. Olfactory glomeruli in the dorsal hemisphere of the olfactory bulb, the dorsal cluster, dorsal ring, anterior plexus, lateral chain, and medial glomeruli, were seen adjacent to 5HT-immunoreactive fibers. However, glomeruli in the ventral hemisphere, the ventral ring, and ventral cluster did not display this association. The presence of specific glomerular territories and discrete glomerular subsets with substances relevant to olfactory sensory neuron activity suggest a spatial organization of information flow in the lamprey olfactory pathway.
Frontini
Andrea
A
Department of Biological Sciences, University of Windsor, Windsor, Ontario N9B 3P4, Canada.
Zaidi
Aliya U
AU
Hua
Hong
H
Wolak
Tomasz P
TP
Greer
Charles A
CA
Kafitz
Karl W
KW
Li
Weiming
W
Zielinski
Barbara S
BS
D000818:Animals; D005075:Biological Evolution; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D005456:Fluorescent Dyes; D044385:GTP-Binding Protein alpha Subunits; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007798:Lampreys / Q000033:anatomy & histology* / Q000254:growth & development / Q000502:physiology; D007814:Larva / Q000166:cytology* / Q000254:growth & development / Q000502:physiology; D037102:Lectins; D009475:Neurons, Afferent / Q000166:cytology* / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development / Q000502:physiology; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure*; D012701:Serotonin / Q000378:metabolism; D012903:Smell / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology
D005456:Fluorescent Dyes; D044385:GTP-Binding Protein alpha Subunits; D037102:Lectins; C084618:olfactory G protein subunit alpha olf; D012701:Serotonin; D020962:Heterotrimeric GTP-Binding Proteins