A single nucleotide polymorphism in the E-cadherin gene promoter-160 is not associated with risk of Korean gastric cancer.
Recently, the -160 C/A polymorphism, located within the regulatory region of Ecadherin promoter, has been shown to influence E-cadherin transcription by altering transcription factor binding. We examined the effect of this polymorphism on risk of gastric cancer and on histological classification of intestinal- and diffuse-type gastric cancer in 146 normal healthy individuals and 292 Korean gastric cancer patients. Genomic DNA samples were examined by polymerase chain reaction (PCR)-single strand conformational polymorphism (SSCP)-sequencing and confirmed by restriction fragment length polymorphism (RFLP). Unexpectedly, there was no significant difference in the genotype frequencies of the polymorphism between normal control and gastric cancer patients (x(2) test, p=0.433). The estimated odd ratio of C/C to A/A genotype in gastric cancer cases was 1.07 (95% confidence interval, 0.396-2.870). We also found no evidence for differences in risk for the intestinal- and diffuse-type gastric cancer. These results suggest that the -160 C/A polymorphism of the E-cadherin has no direct effect on the risk of Korean gastric cancer development and on its histological classification.
Park
Won Sang
WS
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Cho
Yong Gu
YG
Park
Jik Young
JY
Kim
Chang Jae
CJ
Lee
Jong Heun
JH
Kim
Hong Sug
HS
Lee
Jong Woo
JW
Song
Young Hwa
YH
Park
Cho Hyun
CH
Park
Yong Kyu
YK
Kim
Su Young
SY
Nam
Suk Woo
SW
Lee
Sug Hyung
SH
Yoo
Nam Jin
NJ
Lee
Jung Young
JY
D000483:Alleles; D015820:Cadherins / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007723:Korea; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic; D012306:Risk; D013274:Stomach Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic
D015820:Cadherins; D004247:DNA