Rat neuronal cells in primary culture as a model for nociceptin/orphanin FQ metabolism.
Cortical cells in primary culture contain active proteolytic enzymes capable of cleaving nociceptin/orphanin FQ (N/OFQ) and dynorphins to shorter, bioactive fragments. Cells were divided into subpopulations (neurons, astrocytes and oligodendrocytes), and the metabolic pathways in particular populations of the cells were investigated. Processing of N/OFQ and dynorphins in the cytosolic fractions differed significantly. The proposed cell model can be used for the more detailed studies on the central nervous system proteomics.
Suder
Piotr
P
Regional Laboratory, Jagiellonian University, Krakow, Poland.
Bogusiewicz
Anna
A
Rolka
Krzysztof
K
Laidler
Piotr
P
Kotlinska
Jolanta
J
Silberring
Jerzy
J
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004399:Dynorphins / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D000094942:Nociceptin Receptor
D004399:Dynorphins; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D011957:Receptors, Opioid; D000094942:Nociceptin Receptor; C000722285:Oprl1 protein, rat