title: Short- and long-range effects of Sonic hedgehog in limb development.
abstract: The secreted protein Sonic hedgehog (Shh) and its transmembrane receptor Patched (Ptc) control a major signal transduction pathway in early vertebrate limb development. Ligand-free Ptc interacts with the transmembrane protein Smoothened (Smo) and blocks expression of Smo-controlled genes including ptc. Ligand-bound Ptc removes the block and leads to further expression of ptc, which in turn restricts the range of Shh transport. Currently it is not certain that Shh functions as a morphogen on the 300-microm scale of early chick limb development, because it has been difficult to determine how far different forms of Shh are transported. We develop a model to study the effects of two forms of Shh used experimentally and propose a mechanism for Shh signal transduction based on a two-state model for the Ptc-Smo interaction. Recent bead- and tissue-implant experiments can be explained by using this model without postulating different diffusivities for the two forms of Shh; a difference in other parameters such as the rate of release of Shh from the bead or transplant can explain the results equally well. The model also predicts that lower concentrations of Shh in a bead will produce a response similar to that after a tissue transplant. Our results provide an explanation for the counterintuitive experimental results and show that the same signal transduction mechanism can explain both short- and long-range Shh signaling. We conclude that Shh can function as a long-range morphogen.
authors.affiliation: Department of Mathematics, Washington State University, Pullman, WA 99164, USA. dillon@math.wsu.edu
authors.forename: Robert
authors.initials: R
authors.lastname: Dillon
authors.forename: Chetan
authors.initials: C
authors.lastname: Gadgil
authors.forename: Hans G
authors.initials: HG
authors.lastname: Othmer
mesh_terms: D000818:Animals; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D053823:Hedgehog Proteins; D008433:Mathematics; D008565:Membrane Proteins / Q000502:physiology; D008954:Models, Biological; D009024:Morphogenesis; D000072079:Patched Receptors; D011956:Receptors, Cell Surface / Q000502:physiology; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction; D015534:Trans-Activators / Q000502:physiology*
chemical_list: D053823:Hedgehog Proteins; D008565:Membrane Proteins; D000072079:Patched Receptors; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015534:Trans-Activators