title: [Effects of bcr/abl fusion gene on expression of beta1 integrin and L-selectin in mouse chronic myeloid leukemia cells].
abstract: OBJECTIVE
  To explore the effects of p210 bcr/abl fusion gene on expression of beta1 integrin and L-selectin mRNAs in mouse chronic myeloid leukemia (CML) cells.
  
  
  METHODS
  Comparisons of beta1 integrin and L-selectin mRNA levels among p210 bcr/abl negative, p210 bcr/abl positive, and p210 bcr/abl-Rb-C-Box positive cells were undertaken by quantity RT-PCR.
  
  
  RESULTS
  In p210 bcr/abl positive cells, L-selectin mRNA level was decreased, but beta1 integrin mRNA expression had no change as compared to those in p210 bcr/abl negative cells. When inhibition of bcr-abl tyrosine kinase activity by Rb-C-Box, the L-selectin mRNA expression restored to normal (similar to p210 bcr/abl negative cells).
  
  
  CONCLUSIONS
  p210 bcr/abl oncoprotein inhibits expression of L-selectin mRNA, but not of beta1 integrin mRNA.
authors.affiliation: Department of Hematology, Beijing Hospital, Beijing 100730, China.
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Wang
authors.forename: Ti
authors.initials: T
authors.lastname: Shen
authors.forename: Yu-rong
authors.initials: YR
authors.lastname: Hui
authors.forename: Xi-chun
authors.initials: XC
authors.lastname: Gu
authors.forename: Rong-sheng
authors.initials: RS
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D016161:Genes, Retinoblastoma / Q000235:genetics; D019012:Integrin beta1 / Q000235:genetics*; D019041:L-Selectin / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D019012:Integrin beta1; D012333:RNA, Messenger; D019041:L-Selectin; D016044:Fusion Proteins, bcr-abl