Spectroscopic and electrochemical studies of DNA breakage induced by dopamine and copper ion.
Dopamine may show some biological activities in antitumor and cell apoptosis. Herein, we attempted to employ UV-Vis, CD, and electrochemical methods to investigate the interaction between DNA and dopamine. Both the spectroscopic and electrochemical evidence indicated that dopamine, which was a cation, could interact with polyanion DNA. However, this kind of interaction, mainly by a static electronic force, did not result in any damage of the DNA structure. This situation was quite different when Cu ion was added to the dopamine-DNA system; an obvious change in the color of the solution and in the spectra of UV-Vis and CD showed that some chemical changes occurred in this system, and that the DNA native structure was destroyed. The results of gel electrophores further revealed that DNA might be broken into small segments by dopamine in the presence of Cu ion. The possible reaction mechanisms are discussed.
Liu
Jun
J
National Research Center for Certified Reference Materials, Beijing, 100013, PR China. liujun@nrccrm.com.cn
Li
Qingwen
Q
Yu
Yadong
Y
Fang
Xiang
X
D002942:Circular Dichroism; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D004298:Dopamine / Q000494:pharmacology*; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel; D013056:Spectrophotometry, Ultraviolet
D003300:Copper; D004247:DNA; D004298:Dopamine