Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes.
The DHH1 gene in the yeast Saccharomyces cerevisiae encodes a putative RNA helicase of remarkable sequence similarity to several other DExD/H-box proteins, including Xp54 in Xenopus laevis and Ste13p in Schizosaccharomyces pombe. We show here that over-expression of Xp54, an integral component of the stored messenger ribonucleoprotein (mRNP) particles, can rescue the loss of Dhh1p in yeast. Localization and sedimentation studies showed that Dhh1p exists predominantly in the cytoplasm and is present in large complexes whose sizes appear to vary according to the growth stage of the cell culture. In addition, deletion of dhh1, when placed in conjunction with the mutant dbp5 and ded1 alleles, resulted in a synergistically lethal effect, suggesting that Dhh1p may have a role in mRNA export and translation. Finally, similar to Ste13p, Dhh1p is required for sporulation in the budding yeast. Taken together, our data provide evidence that the functions of Dhh1p are conserved through evolution.
Tseng-Rogenski
Stephanie S-I
SS
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Chong
Jean-Leon
JL
Thomas
Christopher B
CB
Enomoto
Shinichiro
S
Berman
Judith
J
Chang
Tien-Hsien
TH
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D053487:DEAD-box RNA Helicases; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D009154:Mutation; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D029867:Xenopus Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D029543:Nucleocytoplasmic Transport Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D029867:Xenopus Proteins; D049452:Green Fluorescent Proteins; C492151:sum3 protein, S pombe; C492382:DBP5 protein, S cerevisiae; C492152:DED1 protein, S cerevisiae; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases