title: Detection and characterization of 45 kDa platelet activating factor acetylhydrolase in cerebrospinal fluid of children with meningitis.
abstract: Platelet activating factor acetylhydrolase (PAF-AH) activity has been identified in cerebrospinal fluid (CSF) samples taken from children with meningitis. We reported that PAF-AH activity is significantly increased, by about 3 fold, in patients with meningitis compared to control subjects. Because of limited knowledge about this enzyme in CSF, we examined the biochemical properties of CSF PAF-AH. PAF-AH of CSF was calcium independent, showed a broad pH spectrum and was relatively heat stable. In addition, this enzyme activity was strongly inhibited by phenylmethanesulfonyl fluoride (PMSF), partially inhibited by p-bromophenacylbromide (p-BPB), uninhibited by iodoacetamide, and moderately stimulated by dithiothreitol (DTT). PAF-AH of CSF did not degrade phospholipid with a long chain fatty acyl group at sn-2 position. This enzyme hydrolyzed PAF and oxidatively modified phosphatidylcholine. Furthermore, we identified a monomeric polypeptide with a molecular weight of approximately 45 kDa by Western blot using human plasma PAF-AH antibody. These results suggested that plasma type PAF-AH activity exist in CSF taken from children with meningitis.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
authors.forename: Tae Chul
authors.initials: TC
authors.lastname: Moon
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Su Jeong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Lee
authors.forename: Soon Hak
authors.initials: SH
authors.lastname: Kwon
authors.forename: Suk Hwan
authors.initials: SH
authors.lastname: Baek
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000037:antagonists & inhibitors / Q000134:cerebrospinal fluid*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000134:cerebrospinal fluid; D008581:Meningitis / Q000134:cerebrospinal fluid* / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase