HPLC method with UV detection for evaluation of digoxin tablet dissolution in acidic medium after solid-phase extraction.
A simple and reliable method for the evaluation of dissolution of digoxin tablets in 0.01 M hydrochloric acid was developed. Digoxin and its degradation products after solid-phase extraction using C18 Sep-Pak cartridges were evaluated. Analyses were performed on C18 column (LiChrospher RP-18e, 5 microm, 125 x 4.0 mm), as mobile phase water and acetonitrile (72:28, v/v) were used. Detection wavelength was 218 nm. Identity of digoxin degradation products was confirmed by HPLC-MS.
Jedlicka
A
A
Léciva a.s., U kabelovny 130, Dolní Mecholupy, 102 37 Prague 10, Czech Republic. jedlicka@leciva.cz
Grafnetterová
T
T
Miller
V
V
D000465:Algorithms; D002316:Cardiotonic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004077:Digoxin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D002316:Cardiotonic Agents; D007202:Indicators and Reagents; D012996:Solutions; D013607:Tablets; D004077:Digoxin