title: Cloning and identification of the human LPAAT-zeta gene, a novel member of the lysophosphatidic acid acyltransferase family.
abstract: Lysophosphatidic acid (LPA) is a naturally occurring component of phospholipid and plays a critical role in the regulation of many physiological and pathophysiological processes including cell growth, survival, and pro-angiogenesis. LPA is converted to phosphatidic acid by the action of lysophosphatidic acid acyltransferase (LPAAT). Five members of the LPAAT gene family have been detected in humans to date. Here, we report the identification of a novel LPAAT member, which is designated as LPAAT-zeta. LPAAT-zeta was predicted to encode a protein consisting of 456 amino acid residues with a signal peptide sequence and the acyltransferase domain. Northern blot analysis showed that LPAAT-zeta was ubiquitously expressed in all 16 human tissues examined, with levels in the skeletal muscle, heart, and testis being relatively high and in the lung being relatively low. The human LPAAT-zeta gene consisted of 13 exons and is positioned at chromosome 8p11.21.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China. longyu@fudan.edu.cn.|Institute of Genetics, Fudan University, 220 Handan Road, 200433, Shanghai, P.R. China. longyu@fudan.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Shan
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Jinhu
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Dang
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Youheng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, P.R. China.
authors.forename: Shouyuan
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase