Histochemical and immunohistochemical characterization of canine mammary mucinous carcinoma.
Mucinous carcinoma is a rare mammary tumour, characterized by intracellular and extracellular mucin. It is still uncertain whether the origin of the mucin is epithelial, myoepithelial or fibroblastic. Eleven canine cases originally classified as mucinous carcinomas were reassessed and compared with myoepithelial nests of mixed tumours. All samples were examined (1) histochemically by the periodic acid-Schiff (PAS) and PAS-diastase methods, and with alcian blue (pH 2.5 and pH 1.0), mucicarmine and Grimelius silver stain, and (2) immunohistochemically for cytokeratin 19, vimentin, alpha-actin and chromogranin A. This examination revealed that only five of the 11 tumours were genuine mucinous carcinomas. In these five tumours the mucus-secreting cells showed cytoplasmic cytokeratin 19 positivity; the mucus showed PAS-diastase and mucicarmine positivity, and alcianophilia which was stronger at pH 2.5 than at 1.0. The remaining six cases were re-classified as mixed tumours because both mucus and mucus-producing cells shared the following similarities with myoepithelial nests of mixed tumours: vimentin and alpha-actin cytoplasmic positivity, PAS negativity, alcianophilia both at pH 2.5 and 1.0, and mucicarmine positivity.
Brunetti
B
B
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Alma Mater Studiorum Università di Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
Sarli
G
G
Marcato
P S
PS
Benazzi
C
C
D002288:Adenocarcinoma, Mucinous / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D000423:Alcian Blue; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D004285:Dogs; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D007633:Keratins / Q000032:analysis; D015674:Mammary Neoplasms, Animal / Q000737:chemistry* / Q000473:pathology; D018198:Mixed Tumor, Malignant / Q000737:chemistry / Q000473:pathology / Q000662:veterinary; D009077:Mucins / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction / Q000662:veterinary; D013194:Staining and Labeling / Q000662:veterinary
D014408:Biomarkers, Tumor; D009077:Mucins; D007633:Keratins; D000423:Alcian Blue