title: Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray.
abstract: To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
authors.affiliation: Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.
authors.forename: Siti
authors.initials: S
authors.lastname: Khadijah
authors.forename: Soek Ying
authors.initials: SY
authors.lastname: Neo
authors.forename: M S
authors.initials: MS
authors.lastname: Hossain
authors.forename: Lance D
authors.initials: LD
authors.lastname: Miller
authors.forename: S
authors.initials: S
authors.lastname: Mathavan
authors.forename: Jimmy
authors.initials: J
authors.lastname: Kwang
mesh_terms: D000818:Animals; D004267:DNA Viruses / Q000235:genetics*; D033364:Decapoda / Q000821:virology*; D005814:Genes, Viral; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017735:Virus Latency / Q000235:genetics*
chemical_list: D012367:RNA, Viral