[Expression of ATM protein in nasopharyngeal carcinoma cell lines with different radiosensitivity].
OBJECTIVE
It has been reported that ataxia- telangiectasia mutant(ATM) protein is closely correlated with cellular radiosensitivity in several malignant tumors. This study was designed to determine the expression of ATM protein in two nasopharyngeal carcinoma (NPC) cell lines with different radiosensitivity.


METHODS
Two NPC cell lines, CNE1 and CNE2, with different radiosensitivity were established. The localization and quantity of ATM protein were analyzed by fluorescence immunohistochemical method and laser scanning confocal microscope(LSCM).


RESULTS
ATM protein was located in both karyon and cytoplasm, especially strongly expressed in karyon. The intense fluorescence of ATM protein was stronger in karyon of CNE1 than that in CNE2.


CONCLUSIONS
The expression levels of ATM protein differ in CNE1 and CNE2. The variance may be a potential factor, which links with their different radiosensitivity.
Wang
Hong-Mei
HM
Department of Forensic Medicine, Basic Medical College, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Wu
Xin-Yao
XY
Xia
Yun-Fei
YF
Qian
Jian-Yang
JY
D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000532:radiotherapy*; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*; D011836:Radiation Tolerance; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases