title: [Expression of heparanase mRNA and its clinical significance in primary hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the expression of heparanase mRNA and its relation with the clinicopathological features and angiogenesis in primary hepatocellular carcinoma (HCC).
  
  
  METHODS
  Expression of heparanase mRNA was detected by RT-PCR in 51 HCC lesions, and microvessel density (MVD) was detected by immunohistochemical stain with a factor VIII-related monoclonal antibody.
  
  
  RESULTS
  Expression of heparanase mRNA was shown in 49.0% (25/51) HCC lesions. The positive rate of heparanase expression in tumors larger than 3 cm (63.6%, 21/33) was significantly higher than those in smaller tumors (22.2%, 4/18; P < 0.01). Heparanase expression was more frequent in highly invasive tumors (70.0%, 14/20) compared with moderately invasive tumors (46.7%, 7/15) and low invasive ones (25.0%, 4/16; P < 0.05). Moreover, heparanase expression in tumors with high MVD (62.5%, 20/32) was significantly higher than those in tumors with low MVD (26.3%, 5/19; P < 0.05).
  
  
  CONCLUSIONS
  Heparanase mRNA expression may be important for the growth, invasion and angiogenesis of hepatocellular carcinoma.
authors.affiliation: Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: You-lei
authors.initials: YL
authors.lastname: Zhang
authors.forename: Zhi-ren
authors.initials: ZR
authors.lastname: Fu
authors.forename: Guo-shan
authors.initials: GS
authors.lastname: Ding
authors.forename: Hong
authors.initials: H
authors.lastname: Fu
authors.forename: Yuan-he
authors.initials: YH
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D047368:Tumor Burden; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D012333:RNA, Messenger; D000099175:Heparanase