The activities of some glycosaminoglycan-degrading enzymes in uterine leiomyomas.
The activities of some glycosaminoglycan-degrading enzymes in uterine leiomyomas. Both normal human myometrium and uterine leiomyoma contain several glycosaminoglycans (GAGs). In contrast to many normal and tumour tissues the amount of hyaluronic acid (HA) is very low and the proportional amount of sulphated glycosaminoglycans is distinctly higher. We compared the activity of GAG-degrading enzymes in normal myometrium and in uterine leiomyomas. Growth of uterine leiomyomas results in significant reduction in the activities of neutral endoglycosidases degrading most of the sulphated glycosaminoglycans. The activities of acid endoglycosidases also decreased (with the exception of chondroitin-6-sulphate). Thus, the differentiated activity of glycosidases degrading glycosaminoglycans can be a factor modifying the quantity of GAGs.
Wolańska
Małgorzata
M
Department of Medical Biochemistry, Medical Academy of Bialystok, ul. Mickiewicza 2c, 15-230 8, Bialystok, Poland. zdbioch@amb.ac.bialystok.pl
Sobolewski
Krzysztof
K
Cechowska-Pasko
Marzanna
M
Jaworski
Stefan
S
D000328:Adult; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007068:Iduronidase / Q000378:metabolism; D007889:Leiomyoma / Q000201:enzymology*; D008875:Middle Aged; D009215:Myometrium / Q000201:enzymology; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000378:metabolism; D013429:Sulfatases / Q000378:metabolism; D014594:Uterine Neoplasms / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D006025:Glycosaminoglycans; D013429:Sulfatases; D020051:N-Acetylgalactosamine-4-Sulfatase; C018719:N-acetylglucosamine-6-sulfatase; D001616:beta-Galactosidase; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases; D007068:Iduronidase