[The synthesis of MT in rat myocardial fibroblasts].
OBJECTIVE
To study the influence on the synthesis of metallothionein (MT) induced by lipopolysaccharide (LPS), basic fibroblast growth factor (bFGF), oxidized low-density lipoproteins (oxLDL) and ZnCl2 in rat myocardial fibroblasts.


METHODS
The content of MT was measured by 109Cd-Hb saturation method and RT-PCR was used to determine the expression of MT mRNA.


RESULTS
All of the stimulus in this experiment increased the synthesis of MT in myocardial fibroblasts. Compared with control, 1 and 5 mg.L-1 LPS increased the content of MT by 94% and 130% (P < 0.01), 0.2 and 1.0 mg.L-1 bFGF by 41% and 59% (P < 0.01), 2 and 10 mg.L-1 oxLDL by 23% (P < 0.05) and 41% (P < 0.01) respectively. 1,2 and 5 mg.L-1 ZnCl2 increased the content of MT by 66%, 99% and 149% (P < 0.01) in a concentration-dependent manner. 2-8 mg.L-1 ZnCl2 increased the content of MT1 mRNA by 69%-129%, MT2 mRNA by 31%-60% (P < 0.01). But 10 mg.L-1 ZnCl2 didn't stimulate the synthesis of MT more significantly, probably because ZnCl2 of high concentration damaged myocardial fibroblasts and decreased the survival rate of the cells.


CONCLUSIONS
Many stimulus such as LPS, bFGF, oxLDL and ZnCl2 can induce the synthesis of MT, and ZnCl2 can increase the expression of MT mRNA in rat myocardial fibroblasts.
Gao
Lin
L
Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, China.
Wang
Dongyan
D
Li
Yang
Y
Shi
Yanrong
Y
Bu
Dingfang
D
Pang
Yongzheng
Y
Tang
Chaoshu
C
D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008668:Metallothionein / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D016222:Fibroblast Growth Factor 2; D008668:Metallothionein