The influence of calyculin A on lymphocytes in vitro.
Human lymphocytes were cultured in vitro and treated with calyculin A. The aim of this work was to estimate the influence of calyculin A on chromosome morphology and banding patterns. It was also interesting whether calyculin A treatment is useful in cytogenetic analysis of human karyotype. We proved that calyculin A induces chromosome condensation in lymphocytes and raises the mitotic index significantly. Moreover, calyculin A does not influence the banding patterns. Therefore it is concluded that calyculin A can be clinically useful for human karyotyping.
Kowalska
Agata
A
Department of Perinatology and Gynaecology, Silesian Medical Academy, Plac Traugutta 6, 41-800 Zabrze, Poland.
Srebniak
Małgorzata
M
Wawrzkiewicz
Angelika
A
Kamiński
Kazimierz
K
D002871:Chromosome Banding / Q000379:methods; D002875:Chromosomes / Q000187:drug effects*; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008214:Lymphocytes / Q000187:drug effects*; D008387:Marine Toxins; D010080:Oxazoles / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*
D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A; D010749:Phosphoprotein Phosphatases