Early steps of deoxyxylulose phosphate pathway in chromoplasts of higher plants.
1-Deoxy-D-xylulose 5-phosphate and 2C-methyl-D-erythritol 4-phosphate have been shown as intermediates of the deoxyxylulose phosphate pathway used for terpenoid biosynthesis in plants and many microorganisms. In plants this non-mevalonate pathway is located in plastids. In order to investigate the formation of five carbon intermediates, chromoplasts from Capsicum annuum and Narcissus pseudonarcissus were incubated with isotope-labeled 1-deoxy-D-xylulose 5-phosphate or 2C-methyl-D-erythritol 4-phosphate. The downstream metabolites were detected and separated by reversed-phase ion-pair radio-HPLC and their structures elucidated by mass spectroscopy. Here we report the isolation and structural identification of 4-diphosphocytidyl-2C-methyl-D-erythritol and 2C-methyl-D-erythritol 2,4-cyclodiphosphate from chromoplasts; the genes of the corresponding enzymes had been previously identified from Escherichia coli and Arabidopsis.
Fellermeier
Monika
M
Biozentrum der Universität Halle, Weinbergweg 22, D-06120 Halle, Germany.
Sagner
Silvia
S
Spiteller
Peter
P
Spiteller
Michael
M
Zenk
Meinhart H
MH
D001711:Biotransformation; D002212:Capsicum / Q000378:metabolism; D002250:Carbon Radioisotopes; D002338:Carotenoids / Q000096:biosynthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D031427:Narcissus / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010428:Pentosephosphates / Q000737:chemistry / Q000378:metabolism; D018087:Plastids / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013403:Sugar Phosphates / Q000378:metabolism*; D014996:Xylulose / Q000031:analogs & derivatives* / Q000378:metabolism*
C109460:1-deoxylulose 5-phosphate; D002250:Carbon Radioisotopes; D009711:Nucleotides; D010428:Pentosephosphates; D013403:Sugar Phosphates; D002338:Carotenoids; D014996:Xylulose; D004896:Erythritol