[Sequence analysis and genetic polymorphisms of D4S1647 and D6S2414 loci in Chinese Han, Mongolian and Tibetan populations].
OBJECTIVE
To investigate the polymorphisms of two short tandem repeats (STR) loci D4S1647 and D6S2414 in Chinese Han, Mongolian and Tibetan populations.


METHODS
Blood and saliva samples were collected from 308 unrelated individuals in the three populations. DNA was extracted by chelex-100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by nondenaturing polyacrylamide gel electrophoresis and silver staining.


RESULTS
The genotype distributions of D4S1647 and D6S2414 loci were in accordance with Hardy-Weinberg equilibrium. The frequencies of alleles showed no significant differences among the three populations. Analyses of Han families indicated that the alleles agreed with Mendelian inheritance law.


CONCLUSIONS
This study yielded information on the genetic polymorphisms of the two STR loci in Chinese Han, Mongolian and Tibetan populations.
Tang
Zhiyu
Z
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liao
Miao
M
Hu
Yu
Y
Liang
Weibo
W
Zhang
Lin
L
Chen
Guodi
G
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*