Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood.
This is the first report of the direct identification of a HLA-DRB1*03 measles-derived peptide from measles virus infected EBV-transformed B cells. We purified HLA-DR3-peptide complexes from EBV-B cells infected with measles virus (Edmonston strain) and sequenced the HLA-DR3-peptides by mass spectrometry. A class II peptide, derived from a measles phosphoprotein, ASDVETAEGGEIHELLRLQ (P1, residues 179-197), exhibited the capacity to stimulate peripheral blood mononuclear cells to proliferate. Our data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. The approach described herein provides a useful methodology for the future identification of HLA-presented pathogen-derived epitopes using mass spectrometry. The study of cell-mediated immune responses to the measles-derived peptide in immune persons should provide significant insight into the design and development of new vaccines.
Ovsyannikova
Inna G
IG
Mayo Vaccine Research Group, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Johnson
Kenneth L
KL
Naylor
Stephen
S
Muddiman
David C
DC
Poland
Gregory A
GA
D000293:Adolescent; D000595:Amino Acid Sequence; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000032:analysis / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D002648:Child; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology* / Q000378:metabolism*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008458:Measles Vaccine / Q000276:immunology; D008459:Measles virus / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D000956:Antigens, Viral; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D008458:Measles Vaccine; D010455:Peptides