Perfluorocarbons attenuate oxidative lung damage.
The aim of this study was to investigate the effect of tidal liquid ventilation (TLV) compared to conventional mechanical ventilation (CMV) on oxidative lung damage in the setting of acute respiratory distress syndrome (ARDS). After repeated lung lavages, 10 minipigs were treated with CMV or TLV for 4 hr before the animals were sacrificed. Samples for blood gas analysis and bronchial aspirate samples were withdrawn before the induction of lung injury, and at 10 min, 2 hr, and 4 hr after the beginning of ventilatory support. To assess lung oxidative damage, total hydroperoxide (TH) and advanced oxidation protein product (AOPP) concentrations were measured in bronchial aspirate samples. After 2 and 4 hr of ventilatory support, partial oxygen tension (PaO(2)) and base excess (BE) were significantly higher in the TLV group than in the CMV group, while PaCO(2) was slightly higher, but with no statistical significance. In the CMV group, the AOPP level was significantly higher at 4 hr than at baseline. TH and AOPP bronchial aspirate concentrations were higher in the CMV group than in the TLV group at 2 and 4 hr of ventilation. We conclude that animals treated with TLV showed lower oxidative lung damage compared to animals treated with CMV.
Dani
C
C
Division of Neonatology, Careggi University Hospital of Florence, University of Florence School of Medicine, Viale Morgagni 85, 50134 Florence, Italy. cdani@unifi.it
Costantino
M L
ML
Martelli
E
E
Corno
C
C
Fiore
G B
GB
Buonocore
G
G
Longini
M
M
Di Filippo
A
A
Tozzini
S
S
Rubaltelli
F F
FF
D000818:Animals; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000097:blood; D021061:Liquid Ventilation; D008168:Lung / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D012121:Respiration, Artificial; D012128:Respiratory Distress Syndrome / Q000473:pathology*; D013552:Swine; D013556:Swine, Miniature
D005466:Fluorocarbons; D006861:Hydrogen Peroxide