Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice.
Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.
Pereira
Valéria Rêgo Alves
VR
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
de Lorena
Virginia Maria Barros
VM
Nakazawa
Mineo
M
da Silva
Ana Paula Galvão
AP
Montarroyos
Ulisses
U
Correa-Oliveira
Rodrigo
R
Gomes
Yara de Miranda
Yde M
D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014349:Trypanosoma cruzi / Q000276:immunology*
D000941:Antigens; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; C063362:QF116 antigen, Plasmodium falciparum; C023456:fibrinogen-related antigen