[Study on the gene knockout model mice of Duchenne muscular dystrophy].
OBJECTIVE
With a view to further experimental studies on Duchenne muscular dystrophy (DMD), we investigated the motor function, serum creatine kinase (CK) level and pathological characteristics of muscular tissue in C57, mdx, dystrophin/utrophin gene double knockout (dko)mice.


METHODS
Gene identification was performed for the filial generation of heterozygote of utrophin gene knockout of mdx mice for acquiring mdx and dko mice. The motor function, serum CK level, and the pathology of muscular tissue of C57, mdx and dko mice were compared.


RESULTS
There were significant differences in all detecting aspects between C57 mouse and dko mouse. In the aspects of serum CK level and pathology of muscular tissue, there were differences between C57 mouse and mdx mouse; however, no significant difference in motor function was observed between them.


CONCLUSIONS
Compared with mdx mouse, dko mouse is in a more serious illness state which is nearer to the natural state of DMD. Therefore, dko mouse is a more ideal model for studying the praxiology of DMD.
Chen
Songlin
S
Department of Neurology, First Affiliated Hospital of Zhong Shan University, Guangzhou 510080, China.
Zhang
Cheng
C
Liu
Xiaorong
X
Gao
Ling
L
Zhang
Weixi
W
Huang
Wen
W
Lu
Xilin
X
Wang
Zhanhang
Z
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D003598:Cytoskeletal Proteins / Q000235:genetics; D004195:Disease Models, Animal; D016189:Dystrophin / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018345:Mice, Knockout; D009043:Motor Activity / Q000502:physiology*; D020388:Muscular Dystrophy, Duchenne / Q000097:blood / Q000235:genetics / Q000503:physiopathology; D049411:Utrophin
D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008565:Membrane Proteins; C483600:Utrn protein, mouse; D049411:Utrophin; D003402:Creatine Kinase