Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of human growth hormone.
The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison. The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique. The composite microspheres were spherical in shape (44.6 +/- 2.47 microm), and the PLGA-mannitol microspheres were 39.7 +/- 2.50 microm. Drug-loading efficiency varied from 93.2% to 104%. The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres. FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres. The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.
Capan
Yilmaz
Y
Hacettepe University, Ankara, Turkey.
Jiang
Ge
G
Giovagnoli
Stefano
S
Na
Kyu-Heum
KH
DeLuca
Patrick P
PP
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016503:Drug Delivery Systems; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D019382:Human Growth Hormone; D011100:Polyglycolic Acid