title: Probing protein-tannin interactions by isothermal titration microcalorimetry.
abstract: Isothermal titration microcalorimetry (ITC) has been applied to investigate protein-tannin interactions. Two hydrolyzable tannins were studied, namely myrabolan and tara tannins, for their interaction with bovine serum albumin (BSA), a model globular protein, and gelatin, a model proline-rich random coil protein. Calorimetry data indicate that protein-tannin interaction mechanisms are dependent upon the nature of the protein involved. Tannins apparently interact nonspecifically with the globular BSA, leading to binding saturation at estimated tannin/BSA molar ratios of 48:1 for tara- and 178:1 for myrabolan tannins. Tannins bind to the random coil protein gelatin by a two-stage mechanism. The energetics of the first stage show evidence for cooperative binding of tannins to the protein, while the second stage indicates gradual saturation of binding sites as observed for interaction with BSA. The structure and flexibility of the tannins themselves alters the stoichiometry of the interaction, but does not appear to have any significant affect on the overall binding mechanism observed. This study demonstrates the potential of ITC for providing an insight into the nature of protein-tannin interactions.
authors.affiliation: School of Food Biosciences, The University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6AP, United Kingdom. r.a.frazier@reading.ac.uk
authors.forename: Richard A
authors.initials: RA
authors.lastname: Frazier
authors.forename: Athina
authors.initials: A
authors.lastname: Papadopoulou
authors.forename: Irene
authors.initials: I
authors.lastname: Mueller-Harvey
authors.forename: Dawn
authors.initials: D
authors.lastname: Kissoon
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Green
mesh_terms: D002151:Calorimetry / Q000379:methods*; D005780:Gelatin / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013634:Tannins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins; D013634:Tannins; D012710:Serum Albumin, Bovine; D005780:Gelatin