title: Evaluation of generic chiral liquid chromatography screens for pharmaceutical analysis.
abstract: Two different automated generic liquid chromatography screens for the separation of chiral compounds of pharmaceutical interest have been evaluated. The test set comprised 53 chemically diverse chiral compounds involving 55 enantiomeric pairs from the pharmaceutical industry (i.e. starting materials, synthetic intermediates and drug substances). The first screen utilised four polysaccharide-based columns with five mobile phases and showed enantioselectivity for 87% of the test compounds. The second screen employed three macrocyclic glycopeptide columns with two mobile phases and showed enantioselectivity for 65% of the test compounds. Merging of the two screening procedures resulted in an enantioselectivity for 96% of the chiral compounds. It is anticipated that the systematic use of the automated chiral HPLC screens described in this report will substantially reduce the necessary time for method development of pharmaceutically related chiral analytical methods.
authors.affiliation: Department of Analytical Chemistry, AstraZeneca, Process R&D, SE-151 85 Södertälje, Sweden. margareta.e.andersson@astrazeneca.com
authors.forename: Margareta E
authors.initials: ME
authors.lastname: Andersson
authors.forename: David
authors.initials: D
authors.lastname: Aslan
authors.forename: Adrian
authors.initials: A
authors.lastname: Clarke
authors.forename: Johan
authors.initials: J
authors.lastname: Roeraade
authors.forename: Gunnar
authors.initials: G
authors.lastname: Hagman
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D006020:Glycopeptides / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D006020:Glycopeptides; D004364:Pharmaceutical Preparations