title: Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples.
abstract: The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.
authors.affiliation: Department of Animal Health and Production, Universidade Estadual Paulista Júlio de Mesquita Filho, Curso de Medicina Veterinária, UNESP, campus Araçatuba, Rua Clóvis Pestana, 793, Jd. D. Amélia, Araçatuba, SP 16050-680, Brazil. caris@fmva.unesp.br
authors.forename: Cáris Maroni
authors.initials: CM
authors.lastname: Nunes
authors.forename: Luiz Gustavo Ferraz
authors.initials: LG
authors.lastname: Lima
authors.forename: Camila Santos
authors.initials: CS
authors.lastname: Manoel
authors.forename: Rodrigo Norberto
authors.initials: RN
authors.lastname: Pereira
authors.forename: Mauro Massaharu
authors.initials: MM
authors.lastname: Nakano
authors.forename: José Fernando
authors.initials: JF
authors.lastname: Garcia
mesh_terms: D000818:Animals; D002353:Carrier State / Q000175:diagnosis / Q000469:parasitology; D017931:DNA Primers; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D041202:Taenia saginata / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth