[The influence of warm ischemia injury on viability and posttransplantative outcome of liver graft from non-heart-beating donor in rats].
OBJECTIVE
To investigate the dynamical changes of histology, histochemistry, energy metabolism, liver function and posttransplantive survival of liver graft under different warm ischemia times (WIT) in rats and determine the maximum limitation of liver graft to warm ischemia.


METHODS
According to WIT, the rats were randomized into 7 groups, WIT were 0, 10, 15, 20, 30, 45, 60 minutes respectively. The recovery changes of above-mentioned index were observed or measured after liver transplantation. The graft survival and postoperative complications in each subgroup were analyzed.


RESULTS
Liver graft injury was reversible and gradually resumed normal structure and function after reperfusion when WIT was less than 30 minutes. In terms of graft survival, there was no significant difference between subgroups within 30 WIT. When WIT was prolonged to 45 minutes, the recipients long-term survival was severely insulted, and both function and histological structure of liver graft would develop irreversible damage when WIT was prolonged to 60 minutes.


CONCLUSIONS
These results indicate that rat liver graft could be safely subject to warm ischemia within 30 minutes. The levels of adentriphos (ATP) and energy charge (EC) and the activities of glycogen and enzyme-histochemistry of liver graft and its recovery potency after reperfusion may serve as the important criteria to evaluate the quality of liver graft.
He
Xiao-shun
XS
Transplantation Surgery, the First Affiliated Hospital, Sun Yet-sen University of Medical Sciences, Guangzhou 510080, China.
Ma
Yi
Y
Chen
Gui-hua
GH
Zhang
Jin-xin
JX
Wu
Jin-lang
JL
Liang
Ying-jie
YJ
Lin
Guang-yun
GY
Zhu
Zhen-yu
ZY
Hu
Rui-de
RD
Huang
Jie-fu
JF
D000818:Animals; D006084:Graft Rejection; D006085:Graft Survival; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000009:adverse effects / Q000379:methods; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000401:mortality / Q000473:pathology*