Analysis of ellagitannins and conjugates of ellagic acid and quercetin in raspberry fruits by LC-MSn.
The use of gradient reversed phase HPLC with diode array and MS(n) detection for the analysis of ellagitannins, ellagic acid conjugates and quercetin conjugates in raspberries (Rubus idaeus L.) is described. MS(n) is a particularly powerful tool for the analysis of trace levels of natural products in impure extracts as interpretation of fragmentation patterns, coupled in some instances with knowledge of HPLC retention properties, can facilitate the partial identification of components when reference compounds are unavailable.
Mullen
William
W
Plant Products and Human Nutrition Group, Graham Kerr Building, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
Yokota
Takao
T
Lean
Michael E J
ME
Crozier
Alan
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004610:Ellagic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D011794:Quercetin / Q000032:analysis* / Q000737:chemistry*; D027824:Rosaceae / Q000737:chemistry*; D013634:Tannins / Q000032:analysis* / Q000737:chemistry*
D010936:Plant Extracts; D013634:Tannins; D004610:Ellagic Acid; D011794:Quercetin