title: [Cloning and sequence analysis of the molecular-adjuvant gene of the complement C3d of mouse].
abstract: OBJECTIVE
  Considering that the complement C3d plays a stronger positive role in the control of immunologic system, we constructed the recombinant plasmid of the molecular-adjuvant gene of the complement C3d in order to enhance the immunologic efficacy of the gene vaccine.
  
  
  METHODS
  The main points were amplifying the segment of complement C3d gene by using of PCR; inserting it into PGEM-T; detecting it by digesting with Eco RI and sequencing; the whole length being 897 bp; then sub-cloning it into pVAX1.
  
  
  RESULTS
  The DNA sequencing confirmed the C3d sequence.
  
  
  CONCLUSIONS
  We have successfully cloned the gene of C3d and constructed the recombinant plasmid of pVAX1 C3d.
authors.affiliation: Department of Infecious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Meiqin
authors.initials: M
authors.lastname: Sun
authors.forename: Liansan
authors.initials: L
authors.lastname: Zhao
authors.forename: Shouchun
authors.initials: S
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Taoyou
authors.initials: T
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Yirong
authors.initials: Y
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D015933:Complement C3d / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D017422:Sequence Analysis, DNA; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D019444:Vaccines, DNA; D015933:Complement C3d