[Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro].
To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0  10 ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5  10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5  10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.
Chen
Xiao-Ping
XP
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
Liu
Hong
H
Liu
Shu-Hong
SH
Wu
Yan
Y
Wu
Hai-Tao
HT
Fan
Ming
M
D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology; D055815:Young Adult
D020934:Ciliary Neurotrophic Factor; D011994:Recombinant Proteins