title: [Antitumor effect of gene gun-mediated DNA vaccine pWRG-neu immunization in C57BL/6 mice].
abstract: OBJECTIVE
  To investigate the inhibitory effect of DNA vaccine immunization on neu-overexpressed melanoma growth in prophylactic treatment and anti-lung-metastasis experiments in C57BL/6 mice.
  
  
  METHODS
  pcDNA-neu transfected into B16F10 with transfection reagent Fugene 6, neu-overexpressed cell clone B16F10-neu was selected with limited dilution method. The growth curve was drawn to analyse its proliferating character in vitro. With Helios gene gun system, DNA vaccine pWRG-neu was immunized to 8-week-old C57BL/6 mice in the shaved abdominal skin for 3 times at two-weekly interval. After immunization, the life span was analyzed. Using MTT assay, the cytolysis activity of the DNA immunized mice spleen cells was compared.
  
  
  RESULTS
  One clone of neu-overexpressed B16F10-neu was selected and its proliferating character was the same as B16F10 and B16F10-pcDNA. In prophylactic, treatment and anti-lung-metastasis experiments, gene gun-mediated pWRG-neu immunization could exhibit antitumor effects. The growth and metastasis of neu-overexpressed melanoma was reduced dramatically. The spleen cells of the immuned mice showed cytotoxic T lymphocyte (CTL) activity.
  
  
  CONCLUSIONS
  Gene gun-mediated gene transfer is effective and practicable. DNA vaccine pWRG-neu is potent in preventing subsequent tumor cells challenge, inhibiting the tumor growth and metastasis.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zha
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Fu
authors.forename: Zi-qiang
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D019470:Biolistics; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D018734:Genes, erbB-2; D007114:Immunization; D008175:Lung Neoplasms / Q000517:prevention & control* / Q000556:secondary; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D010957:Plasmids; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D019444:Vaccines, DNA