title: Stereospecific high-performance liquid chromatographic analysis of flurbiprofen: application to pharmacokinetic studies.
abstract: A method of analysis of flurbiprofen (+/- 2-(2-fluoro-4-biphenyl)-propionic acid) in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and tissue distribution. A simple high-performance liquid chromatographic method was developed for simultaneous determination of flurbiprofen enantiomers in rat serum. Serum (0.1 ml) was extracted with 2,2,4-trimethylpentane-isopropanol (95:5, v/v) after addition of the internal standard (IS), S-naproxen and acidification with H(2)SO(4). Separation was achieved on a Chiralpak AD-RH column with UV detection at 247 nm. The calibration curve was linear ranging from 0.05 to 50 microg/ml for each enantiomer. The mean extraction efficiency was >95%. Precision of the assay was <11% (CV), and was within 12.6% at the limit of quantitation (LOQ) (0.05 microg/ml). Bias of the assay was lower than 13.1%, and was within 12.8% at the LOQ. The assay was applied successfully to the in vivo kinetic study of flurbiprofen in rats.
authors.affiliation: Department of Pharmaceutical Sciences and Pharmacology, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA.
authors.forename: Xiao Wei
authors.initials: XW
authors.lastname: Teng
authors.forename: Stephen W J
authors.initials: SW
authors.lastname: Wang
authors.forename: Neal M
authors.initials: NM
authors.lastname: Davies
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D005480:Flurbiprofen / Q000032:analysis* / Q000493:pharmacokinetics*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D012996:Solutions; D005480:Flurbiprofen