Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.
Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.
Gregory
Matthew A
MA
Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom.
Till
Rob
R
Smith
Margaret C M
MC
D000595:Amino Acid Sequence; D001287:Attachment Sites, Microbiological / Q000235:genetics*; D001483:Base Sequence; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000235:genetics*; D013302:Streptomyces / Q000821:virology*; D016662:Virus Integration
D008565:Membrane Proteins