Characterization of anthocyanins from the fruits of baguaçu (Eugenia umbelliflora Berg).
Anthocyanin pigments in the berries of baguaçu (Eugenia umbelliflora Berg), a tropical fruit from Brazil, were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by Amberlite XAD-7 open-column chromatography. Six major anthocyanins were isolated by preparative HPLC, and their chemical structures were identified by spectroscopic methods (TLC, UV-vis, MS, and (1)H NMR). Delphinidin 3-O-beta-glucopyranoside, cyanidin 3-O-beta-glucopyranoside, petunidin 3-O-beta-glucopyranoside, pelargonidin 3-O-beta-glucopyranoside, peonidin 3-O-beta-glucopyranoside, and malvidin 3-O-beta-glucopyranoside were identified. On the basis of chromatographic data the total anthocyanin content was 342 mg/100 g of fresh baguaçu berries. Therefore, the concomitant presence of six anthocyanins in a single plant species makes this product promising as a new pigment source.
Kuskoski
E Marta
EM
Department of Analytical Chemistry, Department of Pharmaceutical and Organic Chemistry, Food Science and Toxicology, Faculty of Pharmacy, The University of Seville, 41012 Seville, Spain.
Vega
José M
JM
Rios
José J
JJ
Fett
Roseane
R
Troncoso
Ana M
AM
Asuero
Agustin G
AG
D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005638:Fruit / Q000737:chemistry*; D006851:Hydrochloric Acid; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D027842:Syzygium / Q000737:chemistry*
D000872:Anthocyanins; D010936:Plant Extracts; D006851:Hydrochloric Acid