title: Enhancement of the sliding velocity of actin filaments in the presence of ATP analogue: AMP-PNP.
abstract: The sliding velocity of actin filaments was found to increase in the presence of ATP analogues. At 0.5 mM ATP, the presence of 2.0 mM of AMP-PNP enhanced the filament velocity from 3.2 up to 4.5 microm/s. However, 2 mM ADP decreased the velocity down to 1.1 microm/s. The results suggest that the complex conformations of myosin cross-bridges interacting with an actin filament in the presence of ATP analogues makes the entire filament move faster.
authors.affiliation: Department of BioEngineering, Nagaoka University of Technology, Nagaoka 940-2188, Japan.
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Sakamaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Honda
authors.forename: Ei'ichi
authors.initials: E
authors.lastname: Imai
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Hatori
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shimada
authors.forename: Koichiro
authors.initials: K
authors.lastname: Matsuno
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000502:physiology*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000737:chemistry; D009218:Myosins / Q000378:metabolism / Q000502:physiology; D011817:Rabbits; D016008:Statistical Distributions
chemical_list: D000266:Adenylyl Imidodiphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009218:Myosins