HPLC analysis of fusaric acid, 9,10-dehydrofusaric acid and their methyl esters, toxic metabolites from weed pathogenic Fusarium species.
A simple and rapid HPLC method, using a high-density C18 column, has been developed for the quantitative analysis of fusaric and dehydrofusaric acids and their methyl esters in the methanol extract of lyophilised culture filtrates of species of Fusarium. The method has been used to determine the content of these metabolites in two strains of Fusarium oxysporum and in strains of F. nygamai and F. udum. Fusaric acid has been isolated and identified from a strain of F. udum for the first time.
Amalfitano
Carmine
C
Dipartimento di Scienze Chimico-Agrarie, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy. amalfita@unina.it
Pengue
Raffaele
R
Andolfi
Anna
A
Vurro
Maurizio
M
Zonno
Maria C
MC
Evidente
Antonio
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005669:Fusaric Acid / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry* / Q000378:metabolism*
C477627:9,10-dehydrofusaric acid; D005669:Fusaric Acid