title: Antifibrinolytic proCPU is present in the peritoneal cavity during surgery.
abstract: The fibrinolytic capacity of the peritoneum plays a pivotal role in peritoneal wound healing. During surgery the balance between fibrin deposition and degradation is tilted towards deposition, leading to the formation of adhesions. In blood, carboxypeptidase U (CPU) stabilizes clots by retarding fibrinolysis. The purpose of this study was to investigate whether the more stable zymogen, proCPU, is also present in the peritoneal cavity and, if so, to examine its origin. Levels of proCPU were measured in plasma and serosal peritoneal fluid collected during surgery. Peritoneal biopsies were stained for proCPU. Two-dimensional gel electrophoresis was performed to study the protein composition of the serosal fluid compared to plasma and Western blotting to identify differences in glycosylation of proCPU, indicating possible different cellular origin. Cultured human mesothelial cells were examined for proCPU production under normal conditions and conditions mimicking surgery. We found comparable and correlating levels of proCPU in serosal fluid and plasma. ProCPU was also found where fibrin covered the injured peritoneal surface. A protein composition very similar in serosal fluid and plasma was shown by two-dimensional gel electrophoresis, and the proCPU pattern did not indicate a different origin. No proCPU production was found in cultured mesothelial cells. This is the first study to report on the presence of proCPU in the peritoneal cavity, which seems to be the result of plasma oozing out during the inflammatory reaction to the surgical trauma. This is likely to be important for the balance between fibrin deposition and degradation and thereby in the formation of postoperative adhesions.
authors.affiliation: Department of Surgery, Sahlgrenska University Hospital/Ostra, Göteborg University, Sweden. stina.falk@surgery.gu.se
authors.forename: K
authors.initials: K
authors.lastname: Falk
authors.forename: P
authors.initials: P
authors.lastname: Björquist
authors.forename: P
authors.initials: P
authors.lastname: Falk
authors.forename: M
authors.initials: M
authors.lastname: Hedgren
authors.forename: M L
authors.initials: ML
authors.lastname: Ivarsson
authors.forename: B
authors.initials: B
authors.lastname: Lanne
authors.forename: O
authors.initials: O
authors.lastname: Panfilov
authors.forename: L
authors.initials: L
authors.lastname: Holmdahl
mesh_terms: D000933:Antifibrinolytic Agents / Q000032:analysis; D001202:Ascitic Fluid / Q000737:chemistry*; D025901:Carboxypeptidase B2 / Q000032:analysis* / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D010529:Peritoneal Cavity / Q000601:surgery*; D010537:Peritoneum / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D000933:Antifibrinolytic Agents; D025901:Carboxypeptidase B2