title: Assessing the separation of neutral plant secondary metabolites by micellar electrokinetic chromatography.
abstract: In this work, partition coefficients (Pwm) and solute-micelle association constants per monomer (Km/N) were measured using micellar electrokinetic chromatography in tetraborate-sodium dodecylsulfate electrolytes for 18 important plant secondary metabolites (coumarin, verbenone, camphor, eucalyptol, carvone, alpha-terpineol, linalool, jasmone, bergapten, rose oxide, geraniol, t-anethole, citronellal, citronellol, p-cymene, limonene, caryophyllene and nerol) of wide occurrence in herbal extracts and essential oils. Caryophyllene presented a retention time longer than anthracene (micelle marker) and its set of constants could not be determined accurately. Pwm and Km/N were generated by the non-linear data fitting of both partition and solute-micelle association models for the 17 solutes under consideration (caryophyllene excluded). Pwm varied from 147 (coumarin) to 13175 (limonene) while Km/N varied from 37 (coumarin) to 3721 (limonene). Under optimal conditions, the separation of the selected compounds was attempted successfully in commercialized samples of rose, anise and geranium essential oils.
authors.affiliation: Institute of Chemistry, University of Sao Paulo, Av. Professor Lineu Prestes 748, 05508-900 Sao Paulo, SP, Brazil.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Micke
authors.forename: Edgar P
authors.initials: EP
authors.lastname: Moraes
authors.forename: João P S
authors.initials: JP
authors.lastname: Farah
authors.forename: Marina F M
authors.initials: MF
authors.lastname: Tavares
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008962:Models, Theoretical; D010944:Plants / Q000737:chemistry*