title: [Immune response in oral and rectal immunization by the attenuated strain of salmonella carrying the HIV DNA-vaccine].
abstract: The recombinant strain of Salmonella typhimurium SL7207/pBMC-env, carrying a plasmid containing the gene of protein HIV-1 gp-160, was obtained under the monitoring by CMV-promoter. The above strain was used in the rectal and oral immunization of BALB/c mice. HIV-specific antibodies were detected in serum after a one-time immunization; such antibodies were able to inhibit the viral replication in vitro. Furthermore, the shaping-up of the specific cytotoxic and of proliferative responses was registered. Finally, the rectal immunization by cells of the Salmonella recombinant strain can be regarded as a promising delivery system of DNA-vaccine (pBMC-env), and it is more effective versus the oral immunization variant.
authors.forename: L I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: N A
authors.initials: NA
authors.lastname: Nekrasova
authors.forename: G M
authors.initials: GM
authors.lastname: Ignat'ev
authors.forename: A P
authors.initials: AP
authors.lastname: Agafonov
authors.forename: T P
authors.initials: TP
authors.lastname: Proniaeva
authors.forename: B V
authors.initials: BV
authors.lastname: Murashev
authors.forename: A E
authors.initials: AE
authors.lastname: Romanovich
authors.forename: N A
authors.initials: NA
authors.lastname: Klimov
authors.forename: A P
authors.initials: AP
authors.lastname: Kozlov
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000285:Administration, Rectal; D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005822:Genetic Vectors / Q000008:administration & dosage; D015483:HIV Antibodies / Q000097:blood; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D015497:HIV-1; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D012486:Salmonella typhimurium / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D019444:Vaccines, DNA