Genotoxic effects of vanadium(IV) in human peripheral blood cells.
Vanadium has been considered an aneuploidogen; however, there is controversial information about the clastogenic effects of vanadium compounds. In this study, the genotoxicity of vanadium(IV) tetraoxide (V(2)O(4)) was evaluated in human cultured lymphocytes and leukocytes using the mitotic index (MI), the replicative index (RI), chromosome aberrations (CA), sister chromatid exchanges (SCE), satellite associations (SA) and the single cell gel electrophoresis (SCGE) assay. This chemical induced a clear dose-response in MI inhibitions and modifications in the RI. In the CA, including breaks and exchanges and in the SCE, a significant increase appeared in the treated group compared with the controls. The SA test did not reveal an important difference. For the detection of genotoxic properties of vanadium(IV) using the SCGE assay, the 2 h evaluation period was not long enough for the chemical to enter the cell. These results indicate that vanadium(IV) tetraoxide is capable of inducing cytotoxic and cytostatic effects and chromosomal damage.
Rodríguez-Mercado
Juan José
JJ
Unidad de Investigación en Genética y Toxicología Ambiental, Bioterio Campo-II, Facultad de Estudios Superiores-Zaragoza, UNAM A.P. 9-020, Mexico 15000, D.F., Mexico.
Roldán-Reyes
Elia
E
Altamirano-Lozano
Mario
M
D000328:Adult; D001681:Biological Assay; D018929:Cell Culture Techniques; D002869:Chromosome Aberrations / Q000139:chemically induced*; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology*; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology*; D008297:Male; D009152:Mutagenicity Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013997:Time Factors; D014639:Vanadium / Q000493:pharmacokinetics / Q000633:toxicity*
D014639:Vanadium