[Relationship between the mutation of insulin receptor substrate-1 gene 3'untranslated region and type 2 diabetes in the Chinese].
OBJECTIVE
To study the relationship between the mutation of the insulin receptor substrate-1 (IRS-1) gene 3'untranslated region and Type 2 diabetes in the Chinese.


METHODS
Samples were obtained from 120 Chinese patients with Type 2 diabetes and 120 control subjects. The 3'untranslated region sequence of IRS-1 gene was screened by the polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. All SSCP variations were submitted to DNA sequence analysis.


RESULTS
The SSCP analysis showed false positive results. No gene mutation was found in any of the subjects.


CONCLUSIONS
Mutation in the IRS-1 gene 3'untranslated region is not an important genetic factor for Type 2 diabetes in the Chinese.
Chen
Shu-hua
SH
Department of Biochemistry, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Gu
Ya-peng
YP
Zeng
Wei-min
WM
D020413:3' Untranslated Regions / Q000235:genetics*; D044466:Asian People; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D010750:Phosphoproteins