Simultaneous determination of D-glucose and 3-hydroxybutyrate by chemiluminescence detection with immobilized enzymes in a flow injection system.
A chemiluminometric flow-through sensor for the simultaneous determination of glucose (Glu) and 3-hydroxybutyrate (HB) in a single sample has been developed. Coimmobilized 3-hydroxybutyrate dehydrogenase/NADH oxidase/peroxidase, a support material, and coimmobilized glucose dehydrogenase/NADH oxidase/peroxidase were packed sequentially in a transparent PTFE tube. The tube was then placed in front of a photomultiplier tube as a flow cell. A two-peak recording was obtained by one injection of the sample solution. The peak heights of the first and second peaks were dependent on the concentrations of HB and Glu, respectively. The calibration graphs for HB and Glu were linear at 0.05-10 and 0.1-30 microM, respectively. The maximum sample throughput was 30 h(-1). The sensor was stable for two weeks.
Kiba
Nobutoshi
N
Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400-8511, Japan. kibat@yamanashi.ac.jp
Inoue
Youhei
Y
Tachibana
Masaki
M
Tani
Kazue
K
Koizumi
Hitoshi
H
D020155:3-Hydroxybutyric Acid / Q000032:analysis*; D015374:Biosensing Techniques; D002138:Calibration; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry; D006884:Hydroxybutyrate Dehydrogenase / Q000737:chemistry; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D006884:Hydroxybutyrate Dehydrogenase; D042843:Glucose 1-Dehydrogenase; D010544:Peroxidases; D009247:NADH, NADPH Oxidoreductases; D005947:Glucose; D020155:3-Hydroxybutyric Acid