Effect of PPX1 inactivation on the exopolyphosphatase spectra in cytosol and mitochondria of the yeast Saccharomyces cerevisiae.
Inactivation of PPX1 encoding exopolyphosphatase PPX1 in Saccharomyces cerevisiae results in a change in the exopolyphosphatase spectrum in the yeast cells. In the PPX1-deficient strain, elimination of an approximately 45 kD exopolyphosphatase is observed in the cytosol, and activity of an exopolyphosphatase with molecular mass of approximately 830 kD increases fivefold. The latter activity differs greatly in properties from the low-molecular-mass enzyme of the parent strain. In the soluble fraction of the mutant mitochondria, exopolyphosphatase of approximately 45 kD characteristic of the soluble mitochondrial fraction in the parent strain is eliminated, and exopolyphosphatase with a molecular mass of approximately 440 to approximately 830 kD is found. On PPX1 inactivation, a membrane-bound form of mitochondrial exopolyphosphatase is unaffected in its activity level and properties. Therefore, the membrane-bound exopolyphosphatase of mitochondria and the high-molecular-mass enzyme of the cytosol of S. cerevisiae are not encoded by the PPX1 gene, unlike the soluble low-molecular-mass exopolyphosphatase of mitochondria, which is probably a product of this gene with a posttranslational modification. In the PPX1 mutant, exopolyphosphatase properties in the cell as a whole undergo modifications including the ability to hydrolyze polyphosphates (polyP) with different polymer degree.
Lichko
L P
LP
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Moscow Region, Russia.
Pestov
N A
NA
Kulakovskaya
T V
TV
Kulaev
I S
IS
D017766:Acid Anhydride Hydrolases / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D002455:Cell Division; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D011755:Pyrophosphatases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology*; D013379:Substrate Specificity
D017766:Acid Anhydride Hydrolases; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C020137:exopolyphosphatase