Rational improvement of centrifugal partition chromatographic settings for the production of 5-n-alkylresorcinols from wheat bran lipid extract. I. Flooding conditions--optimizing the injection step.
A dedicated CPC prototype permits direct flow pattern visualization in the partition cells of a CPC column. It was used to understand "flooding", a frequent phenomenon associated with large injections. A general strategy was developed to optimize the injection step in the framework of a particular preparative separation: the purification of 5-n-alkylresorcinols from a wheat bran lipid extract on a several hundred milligram scale. The construction of the "mobile phase/stationary phase/sample" pseudo ternary diagram characterizes the effect of the injected solution on mobile and stationary phases. The position of the binodal curve maximum indicates if the biphasic system is "robust" towards a large injection or not, and can be used for optimum mass load determination.
Marchal
Luc
L
Laboratoire de Génie des Procédés-Environnement-Agroalimentaire, Université de Nantes, UMR CNRS 6144, CRTT, BP 406-44602, Saint-Nazaire Cedex, France.
Intes
Olivier
O
Foucault
Alain
A
Legrand
Jack
J
Nuzillard
Jean-Marc
JM
Renault
Jean-Hugues
JH
D002498:Centrifugation; D003377:Countercurrent Distribution / Q000379:methods*; D012118:Resorcinols / Q000302:isolation & purification*
D012118:Resorcinols