title: Effect of phosphodiesterase type 3 inhibitor on developmental competence of immature mouse oocytes in vitro.
abstract: In vitro use of arresters of meiosis could improve cytoplasmic maturation of immature oocytes by controlling the period of prophase I. Phosphodiesterases (PDE) are responsible for the breakdown and concomitant inactivation of the cyclic nucleotides cAMP and cGMP and are implicated in the regulation of oocyte meiotic maturation. Selective inhibitors of phosphodiesterase type 3 (PDE3) prevent meiotic resumption of mammalian oocytes. This study evaluated the impact of meiosis arrest by PDE3 inhibitor, Org 9935, on developmental competence of geminal vesicle (GV)-stage oocytes from small antral follicles. Cumulus-oocyte complexes (COC), retrieved from antral follicles 24 h after eCG exposure and cultured in the presence of PDE3 inhibitor (10 microM) for an additional 24 h, remained arrested in the meiotic prophase. The GV configuration of oocytes before and after the arrest by PDE3 inhibitor was examined. After the period of meiosis arrest, a significantly increased proportion of oocytes had acquired a nucleolus surrounded by a condensed chromatin rim at the GV, which is a morphological correlate of transcriptional repression. Removal of inhibitor resulted in 90.6% +/- 8.3% of oocytes with the first polar body extruded. Fertilization was significantly improved in oocytes that had been arrested compared with oocytes collected 24 h after eCG and undergoing in vitro maturation immediately. Embryonic preimplantation and live offspring rates of arrested oocytes were higher, although not significantly, than those of nonarrested oocytes. These results suggest that a temporal block of meiosis by PDE3 inhibitor promotes developmental competence of mice oocytes retrieved from small antral follicles.
authors.affiliation: Follicle Biology Laboratory, Vrije Universiteit Brussel, Brussels, 1090 Belgium. lrianad@az.vub.ac.be
authors.forename: D
authors.initials: D
authors.lastname: Nogueira
authors.forename: R
authors.initials: R
authors.lastname: Cortvrindt
authors.forename: D G
authors.initials: DG
authors.lastname: De Matos
authors.forename: L
authors.initials: L
authors.lastname: Vanhoutte
authors.forename: J
authors.initials: J
authors.lastname: Smitz
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008540:Meiosis; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D002843:Chromatin; D010726:Phosphodiesterase Inhibitors; D013876:Thiophenes; C097915:Org 9935; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3