Vaccenic acid (t11-18:1) is converted to c9,t11-CLA in MCF-7 and SW480 cancer cells.
The aims of this study were to determine whether vaccenic acid (VA; t11-18:1) is converted to c9,t11-CLA in human mammary (MCF-7) and colon (SW480) cancer cell lines and whether VA influences cell viability and other CLA-bioresponsive markers. When cells were incubated in the presence of VA at concentrations of 5 to 20 microg/mL, both VA and c9,t11-CLA increased in cellular lipids in a dose-dependent manner. After 4 d of incubation of SW480 and MCF-7 cells with VA (20 microg/mL), c9,t11-CLA increased from undetectable levels to 8.57 and 12.14 g/100 g FAME in cellular lipids, respectively. VA supplementation for 4 d at 5, 10, and 15 microg/mL had no effect on cell growth, whereas 20 microg/mL significantly (P < 0.05) reduced cell growth in both cell lines. VA (20 microg/mL) treatment induced DNA fragmentation and significantly (P < 0.05) depleted cytosolic GSH levels in the SW480 cell line after 4 d of incubation, suggesting that apoptosis was the mode of cell death induced by VA. Both VA and c9,t11-CLA reduced (P < 0.05) total ras expression in SW480 cells. 14C-Arachidonic acid uptake into the MG fraction was significantly increased (P < 0.05) in both cell lines while uptake into the phospholipid fraction decreased in response to VA. VA treatment significantly (P < 0.05) increased 8-epi-prostaglandin F2alpha in both cell lines. The data indicate that growth suppression and cellular responses of both cells lines are likely mediated by VA desaturation to c9,t11-CLA via delta9-desaturase.
Miller
Aine
A
School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
McGrath
Emma
E
Stanton
Catherine
C
Devery
Rosaleen
R
D017209:Apoptosis / Q000187:drug effects; D016718:Arachidonic Acid / Q000378:metabolism; D001711:Biotransformation; D002250:Carbon Radioisotopes; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D005989:Glycerides / Q000378:metabolism; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000378:metabolism*; D009829:Oleic Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism
D002250:Carbon Radioisotopes; D005227:Fatty Acids; D005989:Glycerides; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D010743:Phospholipids; D011453:Prostaglandins; D016718:Arachidonic Acid; C050413:11-octadecenoic acid