title: Giant cell granuloma of the temporal bone: a case report with immunohistochemical, enzyme histochemical, and in vitro studies.
abstract: A case of giant cell granuloma (GCG) that occurred in the right temporal bone is reported. The lesion showed histologic features identical to GCG. The multinuclear giant cells (MGCs) in the lesion showed strong reactivity with CD68, but patchy staining for myeloid/histiocyte antigen, alpha-1-antitrypsin, alpha-1-antichymotrypsine, and lysozyme. Activity of tartrate-resistant acid phosphatase was also consistently detected in the MGCs. Some of the mononuclear cells of the lesion exhibited similar immunocytochemical and histochemical reactivity as the MGCs. Ki-67 staining, however, was only detected in the mononuclear cells. The MGCs isolated from the lesion presented characteristic morphology of osteoclasts and possessed the ability to excavate bone in vitro. Thus, the MGCs in GCG appeared to express both macrophage- and osteoclast-associated phenotypes. The mononuclear cells were the major proliferative elements in the lesion and a subpopulation of these cells may represent precursors of the MGCs.
authors.affiliation: Department of Oral Pathology, School of Stomatology, Peking University, Beijing, P. R. China.
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Tian
authors.forename: Tie-Jun
authors.initials: TJ
authors.lastname: Li
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Yu
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001847:Bone Diseases / Q000378:metabolism / Q000473:pathology*; D046508:Culture Techniques; D005500:Follow-Up Studies; D006101:Granuloma, Giant Cell / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D000071681:Tartrate-Resistant Acid Phosphatase; D013701:Temporal Bone / Q000737:chemistry / Q000473:pathology*; D000097382:CD68 Molecule
chemical_list: D000135:Acid Phosphatase; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D007527:Isoenzymes; D000071681:Tartrate-Resistant Acid Phosphatase; C067980:CD68 antigen, human; D000097382:CD68 Molecule