Ribozymes: a modern tool in medicine.
Since the discovery of ribozymes and self-splicing introns, it has been estimated that this biological property of RNA combined with other recombinant DNA technologies would become a tool to combat viral diseases and control oncogenes. These goals seem like a distinct possibility now. However, there is still a lot to be learned about the mobility of RNA inside the cells and the cellular factors that can impede ribozyme action in order to capitalize fully on the targeted RNA inactivation property of ribozymes. The most effective approach to maximize ribozyme function in a complex intracellular environment is to understand as much as possible about the intracellular fate of the RNA that is being targeted. As new techniques in cell biology become available, such understanding will be less problematic. Fundamental studies of ribozyme structure and mechanism of catalysis are flourishing both at the academic and industrial level and it can be expected that many new developments will continue to take place in these areas in the near future. Here, we review the design, stability and therapeutic application of these technologies illustrating relevant gene targets and applications in molecular medicine. Relevant problems in implementation of the technology, group I and II introns and the differences in applications, ribozyme structure and the application of this technology to virus attack and oncogene downregulation are discussed. Also some of the latest RNA-based technologies such as siRNA, RNA/DNA duplexes and RNA decoys have been introduced.
Khan
Asad U
AU
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. sunillal@icegeb.res.in
Lal
Sunil K
SK
D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D015316:Genetic Therapy; D006801:Humans; D007438:Introns / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009857:Oncogenes / Q000235:genetics; D016337:RNA, Catalytic / Q000235:genetics* / Q000627:therapeutic use*; D014777:Virus Diseases / Q000628:therapy*
D016337:RNA, Catalytic; C427204:hammerhead ribozyme