title: H2A.Z has a function reminiscent of an activator required for preferential binding to intergenic DNA.
abstract: H2A.Z has been shown to regulate transcription in yeast, and that function resides in its C-terminal region as the reciprocal portion of H2A cannot substitute for the latter. We show that fusion of a transcriptional activating region to the C-terminal region of H2A, which is substituted for that of H2A.Z, can allow the chimera to fulfil the special role of H2A.Z in positive gene regulation, as well as complement growth deficiencies of htz1delta cells. We further show that the 'transcription' function of H2A.Z is linked to its ability to preferentially localize to certain intergenic DNA regions. Our results suggest that H2A.Z modulates functional interactions with transcription regulatory components, and thus increases its localization to promoters where it helps poise chromatin for gene activation.
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Larochelle
authors.forename: Luc
authors.initials: L
authors.lastname: Gaudreau
mesh_terms: D004271:DNA, Fungal / Q000378:metabolism*; D021901:DNA, Intergenic / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006657:Histones / Q000378:metabolism*; D006918:Hydroxyurea / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D006918:Hydroxyurea