Activity-evoked capacitative Ca2+ entry: implications in synaptic plasticity.
The Ca2+ influx controlled by intracellular Ca2+ stores, called store-operated Ca2+ entry (SOC), occurs in various eukaryotic cells, but whether CNS neurons are endowed with SOC capability and how they may operate have been contentious issues. Using Ca2+ imaging, we present evidence for the presence of SOC in cultured hippocampal pyramidal neurons. Depletion of internal Ca2+ stores by thapsigargin caused intracellular Ca2+ elevation, which was prevented by SOC channel inhibitors 2-aminoethoxydiphenyl borate (2-APB), SKF96365, and La3+. Interestingly, these inhibitors also accelerated the decay of NMDA-induced Ca2+ transients without affecting their peak amplitude. In addition, SOC channel inhibitors attenuated tetanus-induced dendritic Ca2+ accumulation and long-term potentiation at Schaffer collateral-CA1 synapses in hippocampal slice preparations. These data suggest a novel link between ionotropic receptor-activated SOC and neuroplasticity.
Baba
Atsushi
A
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
Yasui
Takuya
T
Fujisawa
Shigeyoshi
S
Yamada
Ryuji X
RX
Yamada
Maki K
MK
Nishiyama
Nobuyoshi
N
Matsuki
Norio
N
Ikegaya
Yuji
Y
D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials; D007093:Imidazoles / Q000494:pharmacology; D017136:Ion Transport; D007811:Lanthanum / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000187:drug effects; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
D001896:Boron Compounds; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007093:Imidazoles; D016194:Receptors, N-Methyl-D-Aspartate; D007811:Lanthanum; C109986:2-aminoethoxydiphenyl borate; C063159:1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; D002118:Calcium