Isolation and characterization of simple sequence repeat markers in the hexaploid forage grass timothy (Phleum pratense L.).
To develop simple sequence repeat (SSR) markers for the hexaploid forage grass timothy ( Phleum pratense L.), we used four SSR-enriched genomic libraries to isolate 1,331 SSR-containing clones. All four libraries contained a high percentage of perfect clones, ranging from 78.1% to 91.6%. From these clones, we developed 355 SSR markers when tested from 502 SSR primer pairs. Using all 355 SSR markers we tested one screening panel consisting of eight timothy clones to detect the level of polymorphism and identify a set of loci suitable for framework mapping. The SSR markers detected 90.4% polymorphism between the parents of a pseudo-testcross F(1) population. These SSR markers will provide an ideal marker system to assist with gene targeting, QTL (quantitative trait locus) mapping, and marker-assisted selection in timothy.
Cai
Hong-Wei
HW
Japan Grassland Farming and Forage Seed Association, Forage Crop Research Institute, 388-5, Higashiakada, Nishinasuno, Tochigi 329-2742, Japan. hcai@jfsass.or.jp
Yuyama
Nana
N
Tamaki
Hiroyuki
H
Yoshizawa
Akira
A
D001483:Base Sequence; D017931:DNA Primers; D005819:Genetic Markers; D031784:Phleum / Q000235:genetics*; D011110:Polymorphism, Genetic; D011123:Polyploidy; D040641:Quantitative Trait Loci
D017931:DNA Primers; D005819:Genetic Markers