Auxin action in a cell-free system.
The plant hormone auxin regulates diverse aspects of plant growth and development. Despite its importance, the mechanisms of auxin action remain poorly understood. In particular, the identities of the auxin receptor and other signaling proteins are unknown. Recent studies have shown that auxin acts by promoting the degradation of a family of transcriptional regulators called the Aux/IAA proteins. These proteins interact with another large family of plant-specific transcription factors called Auxin Response Factors (ARF) and negatively regulate their activity. Auxin stimulates Aux/IAA degradation by promoting the interaction between a ubiquitin protein ligase (E3) called SCF(TIR1) and the Aux/IAA protein. In this report, we demonstrate that auxin promotes the interaction between the Aux/IAA proteins and SCF(TIR1) in a soluble extract free of membranes, indicating that this auxin response is mediated by a soluble receptor. In addition, we show that the response is not dependent on protein phosphorylation or dephosphorylation but rather is prevented by an inhibitor of peptidyl-prolyl isomerases.
Dharmasiri
Nihal
N
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Dharmasiri
Sunethra
S
Jones
Alan M
AM
Estelle
Mark
M
D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D002474:Cell-Free System; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009285:Naphthoquinones / Q000494:pharmacology; D019696:Peptidylprolyl Isomerase / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism; D011392:Proline / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism; D012995:Solubility; D014157:Transcription Factors / Q000378:metabolism
C100794:AUX1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D007210:Indoleacetic Acids; D009285:Naphthoquinones; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C083846:auxin-binding protein 1; D011392:Proline; D044843:SKP Cullin F-Box Protein Ligases; D019696:Peptidylprolyl Isomerase; C005134:juglone