Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes.
We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.
McKeen
Catherine M
CM
Department of Chemistry, University of Southampton, Highfield, Southampton, UK S017 1BJ.
Brown
Lynda J
LJ
Nicol
Jamie T G
JT
Mellor
John M
JM
Brown
Tom
T
D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D019793:Fluorescein / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008954:Models, Biological; D008956:Models, Chemical; D015341:Nucleic Acid Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D016133:Polymerase Chain Reaction
D001391:Azo Compounds; D017931:DNA Primers; D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D009841:Oligonucleotides; C008492:methyl red; D019793:Fluorescein