[Fluorescence quenching method for the determination of p-hydroxyphenylpyruvic acid].
A fluorescence quenching method for the determination of p-hydroxyphenylpyruvic acid with trytophan in the medium pH 11.01 NH3-NH4Cl is studied. The calibration curve is linear for p-hydroxyphenylpyruvic acid from 0 to 15 micrograms.mL-1 with lambda ex/lambda em = 285/356 nm. The detection limit is 0.37 microgram.mL-1. Twenty replicate determinations of solutions containing 10 micrograms.mL-1 p-hydroxyphenylpyruvic acid show a relative standard deviation of 1.2%. This method can be applied to the determination of p-hydroxyphenylpyruvic acid in serum with satisfactory results.
Wu
F
F
Institute of Applied Chemistry, Nanchang University, 330047 Nanchang.
Fu
M
M
Wei
X
X
Yang
W
W
Hu
R
R
Guo
L
L
D002138:Calibration; D004735:Energy Transfer; D006801:Humans; D007202:Indicators and Reagents; D010667:Phenylpyruvic Acids / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan
D007202:Indicators and Reagents; D010667:Phenylpyruvic Acids; C010590:4-hydroxyphenylpyruvic acid; D014364:Tryptophan