A novel method for the assessment of pro- and anti-coagulant properties of platelet-derived microvesicles and its usefulness for quality monitoring of platelet haemostatic function.
To investigate the pro- and anti-coagulant properties of platelet-derived microvesicle (MV), a novel procedure for the removal of plasma proteins was designed, which allows determination of these activities in a purified system in the same reaction vessel. While the pro-coagulant activity of the isolated MV increased on day 5 as compared to day 1, the anti-coagulant activity was significantly reduced.
Seghatchian
J
J
Blood Component Technology & Thrombosis/Haemostasis Consultancy, 50 Primrose Hill Road, London NW3 3AA, UK. jseghatchian@btopenworld.com
D001777:Blood Coagulation / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005165:Factor V / Q000378:metabolism; D006405:Hematology / Q000379:methods; D006487:Hemostasis / Q000502:physiology; D011516:Prothrombin / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism; D013997:Time Factors
D005165:Factor V; D011516:Prothrombin; D013917:Thrombin