Enkephalins: Raman spectral analysis and comparison as function of pH 1-13.
Raman spectral studies are carried out on Leu- and Met-enkephalin as a function of the pH value in the range of 1-13. The molecules are dissolved in KCl solvent and the pH is controlled at each value. Spectral analyses reveal the dependence of the structural conformation on the pH, and a comparison of the two molecules is made in three frequency regions: the tyrosine Fermi doublet (850-830 cm(-1)), aromatic side chains (1650-1550 cm(-1)), and carboxylate (1430-1400 cm(-1)). All regions and frequencies are presented, discussed, and compared for the two molecules.
Abdali
S
S
Quantum Protein Centre QUP, Department of Physics, Building 309, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark. abdali@fysik.dtu.dk
Refstrup
P
P
Nielsen
O Faurskov
OF
Bohr
H
H
D045744:Cell Line, Tumor; D002942:Circular Dichroism; D004745:Enkephalins / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D008956:Models, Chemical; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry
D004745:Enkephalins; D014443:Tyrosine; D008715:Methionine; D007930:Leucine