[Inhibition property of a polypeptide APS against phytopathogenic fungi and the possible antifungal mechanism].
OBJECTIVE
To investigate the inhibition property of a novel antifungal polypeptide APS in vitro and its possible antifungal mechanism.


METHODS
The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of APS against different fungi were detected by microdilution test. And the voltaic change of planar lipid bilayer was observed during APS treatment.


RESULTS
APS at low MIC and MBC was shown to have effects on phytopathogenic fungi tested except aspergillus fumigatus, the minimum values of MIC and MBC being 1.43 micrograms/ml and 1.78 micrograms/ml respectively. After APS was put in, the transmembrane current could be recorded.


CONCLUSIONS
APS has strong inhibitory effects on the germination of spores of several phytopathogenic fungi. Its antifungal mechanism is likely to form persistent pores on the cell membrane, thus causing membrane permeation and the death of fungi.
Chen
Gang
G
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Qiu
Xiaoqing
X
Lu
Xiaofeng
X
Pei
Yan
Y
Wei
Qiou
Q
Cheng
Jingqiu
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D008051:Lipid Bilayers; D008826:Microbial Sensitivity Tests; D010455:Peptides
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D008051:Lipid Bilayers; D010455:Peptides