title: Cell wall-associated enzymes in fungi.
abstract: This review compiles and discusses previous reports on the identity of wall-associated enzymes (WAEs) in fungi and addresses critically the widely different terminologies used in the literature to specify the type of bonding of WAEs to other entities of the cell wall compartment, the extracellular matrix (ECM). A facile and rapid fractionation protocol for catalytically active WAEs is presented, which uses crude cell walls as the experimental material, a variety of test enzymes (including representatives of polysaccharide synthases and hydrolases, phosphatases, gamma-glutamyltransferases, pyridine-nucleotide dehydrogenases and phenol-oxidising enzymes) and a combination of simple hydrophilic and hydrophobic extractants. The protocol provides four fully operationally defined classes of WAEs, with constituent members of each class displaying the same basic type of physicochemical interaction with binding partners in situ. The routine application of the protocol to different species and cell types could yield easily accessible data useful for building-up a general objective information retrieval system of WAEs, suitable as an heuristic basis both for the unravelling of the role and for the biotechnological potentialities of WAEs. A detailed account is given of the function played in the ECM by WAEs in the metabolism of chitin (chitin synthase, chitinase and beta-N-acetylhexosaminidase) and of phenols (tyrosinase).
authors.affiliation: Department of Plant Biology, University of Zürich, CH-8008 Zürich, Switzerland. rast@borinst.unizh.ch
authors.forename: Dora M
authors.initials: DM
authors.lastname: Rast
authors.forename: Daniel
authors.initials: D
authors.lastname: Baumgartner
authors.forename: Christoph
authors.initials: C
authors.lastname: Mayer
authors.forename: G O
authors.initials: GO
authors.lastname: Hollenstein
mesh_terms: D002473:Cell Wall / Q000201:enzymology*; D004798:Enzymes / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*; D009172:Mycology / Q000379:methods; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D004798:Enzymes