title: Conversion of pyridylamino sugar chains to corresponding reducing sugar chains.
abstract: Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet reaction. The reaction conditions were optimized so as to obtain the maximal product yield using 1-amino-1-deoxylactose and 1-amino-1-deoxy-N-acetylglucosamine. When the established procedure was successively applied to pyridylamino high-mannose and complex-type sugar chains, the corresponding reducing sugar chains were obtained in yields of 30%.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Takahashi
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakakita
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Hase
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000138:chemical synthesis; D000606:Amino Sugars / Q000737:chemistry*; D000631:Aminopyridines / Q000737:chemistry*; D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D007785:Lactose / Q000138:chemical synthesis; D008358:Mannose / Q000031:analogs & derivatives; D008709:Methenamine / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D013997:Time Factors
chemical_list: D000606:Amino Sugars; D000631:Aminopyridines; D007202:Indicators and Reagents; D007785:Lactose; D008709:Methenamine; D008358:Mannose; D000117:Acetylglucosamine