title: Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima.
abstract: Xylanases catalyze the hydrolysis of the beta-1,4-glycosidic bonds of xylan, which is the second most abundant component of plant cell walls after cellulose. The recombinant xylanase 10B from Thermotoga maritima MSB8 was prepared and crystallized by the sitting-drop vapour-diffusion method using 40 mM zinc acetate, 20 mM MES buffer pH 6.0 and 3% ethanol. Intensity data were collected to 2.5 A resolution at beamline BL26B2 of SPring-8. Preliminary X-ray analysis showed that the crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 77.3, b = 80.6, c = 58.2 A and one molecule per asymmetric unit.
authors.affiliation: Graduate School of Bioscience andBiotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
authors.lastname: Ihsanawati
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kaneko
authors.forename: Chihiro
authors.initials: C
authors.lastname: Morokuma
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D020124:Thermotoga maritima / Q000201:enzymology*
chemical_list: D043364:Endo-1,4-beta Xylanases