Monitoring some phenoxyl-type N-methylcarbamate pesticide residues in fruit juices using high-performance liquid chromatography with peroxyoxalate-chemiluminescence detection.
A systematic investigation of optimal conditions for determining three of the most common phenoxyl-type N-methylcarbamate pesticides (carbaryl, carbofuran and propoxur) in fruit juices by HPLC with peroxyoxalate-chemiluminescence detection is described. The required pre-column hydrolysis of pesticides and derivatization of their hydrolytic metabolites with dansyl chloride was simultaneously carried out in a short time thanks to the micellar catalytic effect provided by cetyltrimethyl ammonium bromide micelles on the hydrolysis step. The liquid chromatographic separation of the dansylated phenols was performed on a reversed-phase C18 column with isocratic elution. The analytes were detected by using an integrated derivatization chemiluminescence detection unit based on the bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide system. Fruit juice samples containing 4.0-1500 microg/l pesticides were analysed with a precision of ca. 6.5%. After contamination of the fruit juice samples, average recovery > 93% at fortification levels of 10-100 microg/l was obtained.
Orejuela
Eva
E
Department of Analytical Chemistry, Campus of Rabanales, University of Córdoba, E-14004 Córdoba, Spain.
Silva
Manuel
M
D001628:Beverages / Q000032:analysis*; D002219:Carbamates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit; D007306:Insecticides / Q000032:analysis*; D008163:Luminescent Measurements; D010070:Oxalates / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
D002219:Carbamates; D007306:Insecticides; D010070:Oxalates; D010573:Pesticide Residues; C044622:peroxyoxalate