The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III.
Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase II from RNA polymerase III with good yield.
Glanc
Paweł
P
Department of Biochemistry and Biotechnology, Agricultural University, Wołyńska 35, 60-637 Poznań, Poland.
Galbas
Mariola
M
Dullin
Piotr
P
Szalata
Marlena
M
Słomski
Ryszard
R
D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D012319:RNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism; D012320:RNA Polymerase III / Q000302:isolation & purification* / Q000378:metabolism; D014908:Triticum / Q000201:enzymology*
D012319:RNA Polymerase II; D012320:RNA Polymerase III