[Exogenous nitric oxide inhibits the proliferation of mouse neural progenitor cells].
OBJECTIVE
To explore the inhibitive effect of exogenous nitric oxide (NO) on the proliferation of mouse neural progenitor cells (NPCs).


METHODS
NPCs were cultured in medium containing EGF, and SNP was added as a donor of NO in vitro. The concentration of NO was measured by the Griess assay; The amount of living cells was measured by MTT assay. The NPCs were induced to differentiate in medium supplemented with serum.


RESULTS
The NO(-)2 concentrations in the supernatants of the experimental groups 1, 2, and 3 were 64.701 +/- 0.606, 82.659 +/- 0.394 and 93.648 +/- 0.394 micro mol/L respectively, 3.099 +/- 0.420 micro mol/L in th control group. After culture with SNP for 24 hours, MTT assay showed the A values of 0.546 +/- 0.016, 0.484 +/- 0.007, and 0.357 +/- 0.007 in the experimental groups 1, 2, and 3 respectively, 0.642 +/- 0.021 in th control group (all P < 0.01). After further culture in the medium without SNP for 24 hours, MTT assay showed the A values of 1.243 +/- 0.036, 1.064 +/- 0.097, and 0.834 +/- 0.031 in the experimental groups 1, 2, and 3 respectively, 1.581 +/- 0.072 in the control group (all P < 0.01). The differences between the A values of the supernatants in the same wells filled with the medium with and without SNP were all significant (P < 0.01). In the medium with serum the NPCs still proliferated actively and differentiated into neuron and glial cell after SNP was removed.


CONCLUSIONS
Exogenous nitric oxide inhibits the proliferation of mice NPC in vitro.
Zhang
Xiang
X
Institute of Neurosurgery of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'An 710032, China.
Lin
Lü-biao
LB
Ji
Xi-tuan
XT
Fei
Zhou
Z
Wu
Jing-wen
JW
Li
Xia
X
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D051379:Mice; D009474:Neurons / Q000166:cytology; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*
D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D004815:Epidermal Growth Factor