[Cbfa1 induces the expression of the mineral-related proteins in human dental papilla cells].
OBJECTIVE
To explicit whether the expression of the mineral-related proteins is regulated by cbfa1 in human dental papilla cells.


METHODS
Human dental papilla cells were cultured in vitro and transfected with pcDNA3-cbfa1 recombinant plasmids. After selected with G418 sulfate, a cell clone named PC-3, which could stably express the cbfa1 mRNA and protein, was proved by PCR and western blot. Then the amount of ALP and OC and the expression of OPN, BSP, ON, DMP1, DSP and DSPP were detected by immunohistochemistry, Western blot and PCR methods.


RESULTS
We established the human dental papilla cells model PC-3 which could stably express the cbfa1 mRNA and protein. Compared with normal cells, a lot of mineral-related proteins such as ALP, OC, OPN, BSP, ON, DMP1 were upregulated in PC-3 cells.


CONCLUSIONS
In human dental papilla cells, cbfa1 can induce the expression of most mineral-related genes and proteins. It may implicate that cbfa1 must play a key role during tooth development and mineralization.
Yu
Qing
Q
Department of Operative Dentistry and Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Xiao
Ming-zhen
MZ
Wu
Bu-ling
BL
Zhu
Qing-lin
QL
Guo
Ting
T
Li
Feng
F
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000502:physiology*; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015675:Osteocalcin / Q000096:biosynthesis*
D050659:Core Binding Factor Alpha 1 Subunit; D015675:Osteocalcin; D000469:Alkaline Phosphatase