Preferential binding of two compatible solutes to the glycan moieties of Peniophora lycii phytase.
Regulation of hydration behavior, and the concomitant effects on solubility and other properties, has been suggested as a main function of protein glycosylation. In this work, we have studied the hydration of the heavily glycosylated Peniophora lycii phytase in solutions (0.15-1.1 m) of the two compatible solutes glycerol and sorbitol. Osmometric measurements showed that glycerol preferentially binds to phytase (i.e., glycerol-glycoprotein interactions are more favorable than water-glycoprotein interactions resulting in a preferential accumulation of glycerol near the protein interface), while sorbitol is preferentially excluded from the hydration sphere (water-glycoprotein interactions are the more favorable). To assess contributions from carbohydrate and peptide moieties, respectively, we compared phytase (Phy) and a modified, yet enzymatically active form (dgPhy) in which 90% of the glycans had been removed. This revealed that both polyols showed a pronounced and approximately equal degree of preferential binding to the carbohydrate moiety. This preferential binding of polyols to glycans is in contrast to the exclusion from peptide interfaces observed here (for dgPhy) and in numerous previous reports on nonglycosylated proteins. Despite the distinct differences between peptide and carbohydrate groups, glycosylation had no effect on the stabilizing action provided by glycerol and sorbitol. On the basis of this, it was concluded that the carbohydrate mantle of Phy is equally accessible in the native and thermally denatured states, respectively (most likely fully accessible in both), and thus that its interactions with compatible solutes have little or no effect on conformational equilibria of the glycoprotein. For solubility and aggregation equilibria, on the other hand, the results suggest a polyol-induced stabilization of monomeric forms.
Bagger
Heidi L
HL
Department of Life Sciences and Chemistry, Roskilde University, Building 18.1, P.O. Box 260, DK-4000 Roskilde, Denmark.
Fuglsang
Claus C
CC
Westh
Peter
P
D010832:6-Phytase / Q000737:chemistry* / Q000378:metabolism*; D000665:Ampholyte Mixtures / Q000737:chemistry; D001236:Aspergillus oryzae / Q000201:enzymology; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D002152:Calorimetry, Differential Scanning; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006031:Glycosylation; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011489:Protein Denaturation; D012995:Solubility; D013012:Sorbitol / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000737:chemistry / Q000378:metabolism
D000665:Ampholyte Mixtures; D005656:Fungal Proteins; D011108:Polymers; D011134:Polysaccharides; C024617:polyol; D014867:Water; D013012:Sorbitol; D010832:6-Phytase; D005990:Glycerol