Ultrastructural analysis of DNA complexes during transfection and intracellular transport.
We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-micro m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.
Cartier
Régis
R
Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
Velinova
Maria
M
Lehman
Cathleen
C
Erdmann
Bettina
B
Reszka
Regina
R
D001692:Biological Transport; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D014162:Transfection; D014407:Tumor Cells, Cultured
D010455:Peptides; D008156:Luciferases