title: Genome-based design of a cell-free culture medium for Tropheryma whipplei.
abstract: Empirical approaches have guided the development of bacterial cultures. The availability of sequenced genomes now provides opportunities to define culture media for growth of fastidious pathogens with computer modelling of metabolic networks. A key issue is the possibility of growing host-dependent bacteria in cell-free conditions. The sequenced Tropheryma whipplei genome was analysed to identify specific metabolic deficiencies. We used this information to design a comprehensive medium that allowed three established T whipplei strains from culture with human cells and one new strain from a clinical sample to grow axenically. Genomic information can, therefore, provide sufficient clues for designing axenic media for fastidious and uncultured pathogens.
authors.affiliation: Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385, Marseille, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Renesto
authors.forename: Nicolas
authors.initials: N
authors.lastname: Crapoulet
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ogata
authors.forename: Bernard
authors.initials: B
authors.lastname: La Scola
authors.forename: Guy
authors.initials: G
authors.lastname: Vestris
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Claverie
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D000193:Actinomycetales Infections / Q000235:genetics / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA; D008061:Whipple Disease / Q000235:genetics / Q000382:microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial