[Gene expression changes after heat shock by cDNA array analysis].
OBJECTIVE
To study gene expression changes of cells in response to heat stress, we isolated total RNA from Jurkat cells before and after heat shock treatment.


METHODS
cDNA was labeled with alpha-32P-dATP during reverse transcription of RNA and then used as probe to cDNA expression array. Autoradiogram images were analyzed by ESTblot software.


RESULTS
After heat shock, the expression level of some forty genes increased while that of sixteen genes decreased. In addition to the elevated expression of heat shock genes, expression of c-Jun and CLK-1 increased most remarkably. The genes with notably decreased expression were integrin alpha-4 and transforming growth factor beta. The elevated expression of c-Jun and hsp90 alpha was further confirmed by Northern blot analysis.


CONCLUSIONS
The expression of some genes in Jurkat cells changes after heat shock treatment. Heat shock induces elevated expression of hsp, c-Jun, CLK-1 gene while decreases expression of integrin alpha-4 and transforming growth factor beta.
Wang
T Y
TY
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Wu
N H
NH
Shen
Y F
YF
D015152:Blotting, Northern; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D015870:Gene Expression; D016758:Genes, jun / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D039001:Integrin alpha Chains / Q000235:genetics; D019169:Jurkat Cells; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics
C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D006360:Heat-Shock Proteins; D039001:Integrin alpha Chains; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta