Ginsenoside F1 protects human HaCaT keratinocytes from ultraviolet-B-induced apoptosis by maintaining constant levels of Bcl-2.
Ginsenosides, the major active ingredients of ginseng, show a variety of biomedical efficacies such as antiaging and antioxidation. Here, we investigate the protective activity of the ginsenoside F1, an enzymatically modified derivative of ginsenoside Rg1, against ultraviolet-B-induced damage in human HaCaT keratinocytes. Ginsenoside F1 significantly reduced ultraviolet-B-induced cell death and protected HaCaT cells from apoptosis caused by ultraviolet B irradiation. Furthermore, ginsenoside F1 prevented ultraviolet-B-induced cleavage of poly(ADP-ribose) polymerase in HaCaT cells. In search of the molecular mechanism responsible for the antiapoptotic effect of ginsenoside F1, we find that protection from ultraviolet-B-induced apoptosis is tightly correlated with ginsenoside-F1-mediated inhibition of ultraviolet-B-induced downregulation of Bcl-2 and Brn-3a expression.
Lee
Enn Hee
EH
R & D Center, AmorePacific Corporation, 314-1 Bora-ri, Giheung-eup, Yongin-si, Kyeonggi-do 449 729, Korea.
Cho
Si Young
SY
Kim
Su Jong
SJ
Shin
Eui Seok
ES
Chang
Hui Kyoung
HK
Kim
Duck Hee
DH
Yeom
Myeong Hoon
MH
Woe
Kwang Sik
KS
Lee
Jinseon
J
Sim
Young Chul
YC
Lee
Tae Ryong
TR
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000528:radiation effects; D000078404:Epidermal Cells; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D011401:Promoter Regions, Genetic / Q000502:physiology / Q000528:radiation effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
D004268:DNA-Binding Proteins; D036145:Ginsenosides; C494188:POU4F1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors; C055327:ginsenoside F1