Post intoxicative therapeutic immunization with myelin oligodendrocyte glycoproteine (MOG 35-55) suppresses spontaneous regeneration of dopaminergic neurons injured with 1-methyl-4 phenyl-1,2,3,6-tetrahydropiridine (MPTP).
The pathological process of neurodegeneration is accompanied by an inflammatory reaction that is believed to contribute to the pathogenesis of neurodegenerative diseases. The aim of our study was to evaluate the influence of autoimmune reaction induced by post-traumatic vaccination with myelin self-antigen on spontaneous regeneration of dopaminergic neurons, injured with MPTP. C57BL mice were intoxicated with 40 mg/kg MPTP and seven days later immunized with MOG 35-55 peptide in CFA. On the 7th day following intoxication, the MPTP treated mice showed decrease of the dopamine level by 63% as compared to the control mice. However, starting from the 14th day following intoxication, a spectacular increase of dopamine content was observed. Immunization with MOG resulted in a statistically significant reduction of the increase in striatum as compared to non-immunized animals, and was lower by 23%, 17% and 15% on days 14, 28 and 50, respectively. Our results show suppressive influence of autoimmune reaction induced after injury on regeneration of dopamine cells intoxicated with MPTP.
Balkowiec-Iskra
Ewa
E
Department of Experimental and Clinical Pharmacology, Warsaw Medical University, Warsaw, Poland.
Kurkowska-Jastrzebska
Iwona
I
Joniec
Ilona
I
Czlonkowska
Anna
A
Czlonkowski
Andrzej
A
D000818:Animals; D004298:Dopamine / Q000502:physiology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000628:therapy; D006023:Glycoproteins / Q000276:immunology* / Q000494:pharmacology; D007114:Immunization; D007167:Immunotherapy / Q000379:methods*; D020267:MPTP Poisoning / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein; D009416:Nerve Regeneration / Q000276:immunology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology
D006023:Glycoproteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D004298:Dopamine