title: The small RNA profile during Drosophila melanogaster development.
abstract: Small RNAs ranging in size between 20 and 30 nucleotides are involved in different types of regulation of gene expression including mRNA degradation, translational repression, and chromatin modification. Here we describe the small RNA profile of Drosophila melanogaster as a function of development. We have cloned and sequenced over 4000 small RNAs, 560 of which have the characteristics of RNase III cleavage products. A nonredundant set of 62 miRNAs was identified. We also isolated 178 repeat-associated small interfering RNAs (rasiRNAs), which are cognate to transposable elements, satellite and microsatellite DNA, and Suppressor of Stellate repeats, suggesting that small RNAs participate in defining chromatin structure. rasiRNAs are most abundant in testes and early embryos, where regulation of transposon activity is critical and dramatic changes in heterochromatin structure occur.
authors.affiliation: Department of Animal Molecular Genetics, Institute of Molecular Genetics, Kurchatov sq. 2, 123182, Moscow, Russia. ttuschl@rockefeller.edu
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Aravin
authors.forename: Mariana
authors.initials: M
authors.lastname: Lagos-Quintana
authors.forename: Abdullah
authors.initials: A
authors.lastname: Yalcin
authors.forename: Mihaela
authors.initials: M
authors.lastname: Zavolan
authors.forename: Debora
authors.initials: D
authors.lastname: Marks
authors.forename: Ben
authors.initials: B
authors.lastname: Snyder
authors.forename: Terry
authors.initials: T
authors.lastname: Gaasterland
authors.forename: Jutta
authors.initials: J
authors.lastname: Meyer
authors.forename: Thomas
authors.initials: T
authors.lastname: Tuschl
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000502:physiology
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering; D012313:RNA