title: In vitro effect of platelet-derived growth factor-BB on collagen synthesis and proliferation of human periodontal ligament cells.
abstract: OBJECTIVE
  Platelet-derived growth factor (PDGF)-BB is a polypeptide growth factor which has been shown to stimulate periodontal regeneration. In this study, we investigated the time- and dose-dependent effect of PDGF-BB on the proliferation and collagen synthesis of human periodontal ligament (PDL) cells.
  
  
  METHODS
  For the proliferation assay, PDL cells were cultured in 0.01-10 ng ml(-1) of PDGF-BB for 12 or 24 h, and cell numbers were counted. For the collagen synthesis assay, PDL cells were cultured in 0.1-10 ng ml(-1) of PDGF-BB for 1 to 24 h. The ratio of collagen content in total protein was evaluated, and the gene expression of type I collagen was assessed quantitatively by Northern blotting analysis.
  
  
  CONCLUSIONS
  PDGF-BB stimulated the proliferation of PDL cells in a time- and dose-dependent manner with the maximum effect at 10 ng ml(-1). PDGF-BB induced the collagen synthesis of PDL cells with the maximum effect for 24-h treatment, and 1 ng ml(-1) of PDGF-BB. PDGF-BB exhibits an inverse dose-dependent effect on proliferation and collagen synthesis by PDL cells. These findings suggest that PDGF-BB is one of the important regulators of the maintenance of the extracellular matrix in PDL, and may play an important role in the regeneration of PDL.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. yasutaka@bb.alles.or.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Ojima
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Kuboki
authors.forename: T
authors.initials: T
authors.lastname: Komori
mesh_terms: D000077214:Becaplermin; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects*; D024042:Collagen Type I / Q000032:analysis / Q000235:genetics; D017364:Collagenases / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D024042:Collagen Type I; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen; D017364:Collagenases