[Effect of push-pull maneuver on distribution of DNA and NO content in rats' brain tissue].
OBJECTIVE
To study the effect of push-pull maneuver on cerebral apoptosis and NO content of rats.


METHODS
Ninety male Wistar rats were randomly divided into the control group, +Gz exposure group and the push-pull maneuver group. The rats were killed after 30 min exposure, 3 h, 12 h, 24 h or 48 h exposure, and the brain tissues were taken. The variation of NO content measured was using chemical colormetry. Then flow cytometric method was adopted to examine the distribution diagram of cortex and hippocampus-cell DNA for determination of percentage of A0 peak, which could express apoptosis degree.


RESULTS
NO content in +Gz exposure group and push-pull group are significantly different from that of control at 30 min, 3 h and 12 h. And the difference between +Gz group and push-pull group are also significant, but not at 24 h and 48 h. Comparing with the control group, the apoptosis amount of cortex and hippocampus-cell of +Gz exposure group and push-pull maneuver group increased obviously at 3 h, 12 h and 24 h, reached its peak at 24 h, and had no remarkable difference at 48 h. The comparison of the apoptosis amount of cortex cell and hippocampus cell between push-pull maneuver group and +Gz exposure group, difference at 3 h and 24 h and remarkably different at 12 h.


CONCLUSIONS
High +Gz and push-pull maneuver may increase the percentage of cerebral apoptosis amount and NO content. Comparing with the +Gz exposure group, the push-pull maneuver group will cause more increase in apoptosis amount and NO content, but this damage is reversible.
Jiang
Li-ping
LP
Department of Physiology, Jilin Military Medical College, The Fourth Military Medical University, Jilin, China.
Li
Yun-yi
YY
Tong
Wen-ge
WG
Yao
Ping
P
Song
Yu-guo
YG
Sha
Jan-hui
JH
D000337:Aerospace Medicine; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002540:Cerebral Cortex / Q000378:metabolism / Q000502:physiology; D004247:DNA / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D018471:Hypergravity; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D009569:Nitric Oxide; D004247:DNA