title: Prevalence of amebiasis in inflammatory bowel disease in Turkey.
abstract: OBJECTIVE
  To explore the prevalence of amebiasis in inflammatory bowel disease (IBD) in Turkey.
  
  
  METHODS
  In this study, amoeba prevalence in 160 cases of IBD, 130 of ulcerative colitis and 30 of Crohn's disease were investigated in fresh faeces by means of wet mount+Lugol's iodine staining, modified formol ethyl acetate and trichrome staining methods and to compare the diagnostic accuracy of wet mount+Lugol's iodine staining, modified formol ethyl acetate and trichrome staining methods in the diagnosis of Entamoeba histolytica (E. histolytica)/ Entamoeba dispar (E. dispar).
  
  
  RESULTS
  E. histolytica/E. dispar cysts and trophozoites were found in 14 (8.75 %) of a total of 160 cases, 13 (10.0 %) of the 130 patients with ulcerative colitis and 1 (3.3 %) of the 30 patients with Crohn's disease. As for the 105 patients in the control group who had not any gastrointestinal complaints, 2 (1.90 %) patients were found to have E. histolytica /E. dispar cysts in their faeces. Parasite prevalence in the patient group was determined to be significantly higher than that in the control group (Fischer's Exact Test, P<0.05). When the three methods of determining parasites were compared with one another, the most effective one was found to be trichrome staining method (Kruskal-Wallis Test, P<0.01).
  
  
  CONCLUSIONS
  Consequently, amoeba infections in IBD cases have a greater prevalence compared to the normal population. The trichrome staining method is more effective for the detection of E. histolytica /E. dispar than the wet mount+Lugol's iodine staining, modified formol ethyl acetate methods.
authors.affiliation: Department of Gastroenterology, School of Medicine, University of Ege, 35100, Bornova, Izmir, Turkey. sustun@med.ege.edu.tr
authors.forename: Sebnem
authors.initials: S
authors.lastname: Ustun
authors.forename: Hande
authors.initials: H
authors.lastname: Dagci
authors.forename: Umit
authors.initials: U
authors.lastname: Aksoy
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Guruz
authors.forename: Galip
authors.initials: G
authors.lastname: Ersoz
mesh_terms: D000562:Amebiasis / Q000150:complications* / Q000175:diagnosis; D003093:Colitis, Ulcerative / Q000453:epidemiology* / Q000469:parasitology*; D003424:Crohn Disease / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D013194:Staining and Labeling / Q000379:methods; D014421:Turkey / Q000453:epidemiology