title: Expanded substrate screenings of human and Drosophila type 10 17beta-hydroxysteroid dehydrogenases (HSDs) reveal multiple specificities in bile acid and steroid hormone metabolism: characterization of multifunctional 3alpha/7alpha/7beta/17beta/20beta/21-HSD.
abstract: 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) catalyse the conversion of 17beta-OH (-hydroxy)/17-oxo groups of steroids, and are essential in mammalian hormone physiology. At present, eleven 17beta-HSD isoforms have been defined in mammals, with different tissue-expression and substrate-conversion patterns. We analysed 17beta-HSD type 10 (17beta-HSD10) from humans and Drosophila, the latter known to be essential in development. In addition to the known hydroxyacyl-CoA dehydrogenase, and 3alpha-OH and 17beta-OH activities with sex steroids, we here demonstrate novel activities of 17beta-HSD10. Both species variants oxidize the 20beta-OH and 21-OH groups in C21 steroids, and act as 7beta-OH dehydrogenases of ursodeoxycholic or isoursodeoxycholic acid (also known as 7beta-hydroxylithocholic acid or 7beta-hydroxyisolithocholic acid respectively). Additionally, the human orthologue oxidizes the 7alpha-OH of chenodeoxycholic acid (5beta-cholanic acid, 3alpha,7alpha-diol) and cholic acid (5beta-cholanic acid). These novel substrate specificities are explained by homology models based on the orthologous rat crystal structure, showing a wide hydrophobic cleft, capable of accommodating steroids in different orientations. These properties suggest that the human enzyme is involved in glucocorticoid and gestagen catabolism, and participates in bile acid isomerization. Confocal microscopy and electron microscopy studies reveal that the human form is localized to mitochondria, whereas Drosophila 17beta-HSD10 shows a cytosolic localization pattern, possibly due to an N-terminal sequence difference that in human 17beta-HSD10 constitutes a mitochondrial targeting signal, extending into the Rossmann-fold motif.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
authors.forename: Naeem
authors.initials: N
authors.lastname: Shafqat
authors.forename: Hanns-Ulrich
authors.initials: HU
authors.lastname: Marschall
authors.forename: Charlotta
authors.initials: C
authors.lastname: Filling
authors.forename: Erik
authors.initials: E
authors.lastname: Nordling
authors.forename: Xiao-Qiu
authors.initials: XQ
authors.lastname: Wu
authors.forename: Lars
authors.initials: L
authors.lastname: Björk
authors.forename: Johan
authors.initials: J
authors.lastname: Thyberg
authors.forename: Eva
authors.initials: E
authors.lastname: Mårtensson
authors.forename: Samina
authors.initials: S
authors.lastname: Salim
authors.forename: Hans
authors.initials: H
authors.lastname: Jörnvall
authors.forename: Udo
authors.initials: U
authors.lastname: Oppermann
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D015094:3-Hydroxyacyl CoA Dehydrogenases; D000595:Amino Acid Sequence; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001665:Binding Sites; D019556:COS Cells; D004331:Drosophila melanogaster / Q000201:enzymology; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D007700:Kinetics; D008928:Mitochondria / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013256:Steroids / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001647:Bile Acids and Salts; D012739:Gonadal Steroid Hormones; D007527:Isoenzymes; D013256:Steroids; C479399:17 beta-hydroxysteroid dehydrogenase type 10, Drosophila; D015067:17-Hydroxysteroid Dehydrogenases; D015094:3-Hydroxyacyl CoA Dehydrogenases; C108634:HSD17B10 protein, human; C458480:Hsd17b10 protein, mouse; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase