title: Binding of live conidia of Aspergillus fumigatus activates in vitro-generated human Langerhans cells via a lectin of galactomannan specificity.
abstract: Aspergillus fumigatus is the most common aetiological fungus responsible for human pulmonary aspergilloses. This study investigated the primary contact between Langerhans cells (LC), corresponding to dendritic cells present in pulmonary mucosa and live conidia of A. fumigatus. LC play a key role in antigen presentation for initiation of the primary T cell response. In vitro-generated LC (iLC) were differentiated from cultured human cord blood CD34+ cells and incubated at 4 degrees C or 37 degrees C with fluorescein-isothiocyanate (FITC)-stained conidia or control latex beads. In vitro, conidia were shown by microscopy and cytometry to adhere to iLC in a dose- and time-dependent manner. This adhesion was not limited to iLC because interstitial dendritic and other cells also fluoresced in the presence of conidia-FITC. A lectin other than mannose receptor-type lectin was demonstrated to be responsible of conidial binding. Inhibition of binding was observed with heterologous galactomannan and EDTA, indicating a C-lectin-like receptor with galactomannan structure specificity. After binding only a few conidia were internalized in acidic vesicles, as indicated by the cessation of conidial fluorescence. Conidial binding was followed by activation and maturation of iLC, suggesting that LC present in the lung may play a role in cellular host defence against aspergilloses.
authors.affiliation: EA 3087, Université Claude Bernard, Lyon, France and Unité INSERM 346, Lyon, France. florence.persat@univ.Lyon1.fr
authors.forename: F
authors.initials: F
authors.lastname: Persat
authors.forename: N
authors.initials: N
authors.lastname: Noirey
authors.forename: J
authors.initials: J
authors.lastname: Diana
authors.forename: M-J
authors.initials: MJ
authors.lastname: Gariazzo
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
authors.forename: S
authors.initials: S
authors.lastname: Picot
authors.forename: C
authors.initials: C
authors.lastname: Vincent
mesh_terms: D001228:Aspergillosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000378:metabolism*; D002448:Cell Adhesion; D004492:Edetic Acid / Q000378:metabolism; D005434:Flow Cytometry; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D007801:Langerhans Cells / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008168:Lung / Q000276:immunology*; D008172:Lung Diseases, Fungal / Q000276:immunology*; D008351:Mannans / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008985:Monensin / Q000378:metabolism; D009092:Mucous Membrane / Q000276:immunology; D010587:Phagocytosis; D011485:Protein Binding
chemical_list: D037102:Lectins; D008351:Mannans; C012990:galactomannan; D008985:Monensin; D004492:Edetic Acid; D005690:Galactose