An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage.
A two-plasmid dual reporter Escherichia coli biosensor was developed using the genes for bacterial bioluminescence and a mutant of the green fluorescent protein, GFPuv4. To achieve this, the two plasmids, which were derivatives of pBR322 and pACYC184, had compatible origins of replication and different antibiotic selection markers: ampicillin and tetracycline. The parent strains DK1 and ACRG43, each carrying a single plasmid with one of the fusion genes (strain DK1 harboring a fusion of the katG promoter to the lux operon while in ACRG43, the recA promoter was fused with the GFP gene), were responsive to oxidative and DNA damage, respectively, resulting in higher bioluminescence or fluorescence under the relevant toxic conditions. The responses of the dual sensor strain, DUAL22, to various toxicants, e.g., mitomycin C, N-methyl-N-nitro--nitrosoguanidine, hydrogen peroxide and cadmium chloride, were characterized and compared with the responses of the parent strains to the same chemicals. Finally, several chemical mixtures that cause various stress responses were tested to demonstrate the ability of this biosensor to detect specific stress responses within a multiple toxicity environment.
Mitchell
R J
RJ
National Research Laboratory on Environmental Biotechnology, Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology, 1 Oryong-dong, Puk-gu, 500-712 Kwangju, South Korea.
Gu
M B
MB
D019677:Artificial Gene Fusion; D015374:Biosensing Techniques; D019256:Cadmium Chloride / Q000633:toxicity; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005453:Fluorescence; D005798:Genes, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide / Q000633:toxicity; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D018384:Oxidative Stress; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018741:Replication Origin; D012641:Selection, Genetic
D029968:Escherichia coli Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D008769:Methylnitronitrosoguanidine; D049452:Green Fluorescent Proteins; D016685:Mitomycin; D006861:Hydrogen Peroxide; D002374:Catalase; C051253:katG protein, E coli; D008156:Luciferases; D011938:Rec A Recombinases; D019256:Cadmium Chloride