title: Flow cytometric mapping of the leukotriene B4 receptor, BLT1, in human bone marrow and peripheral blood using specific monoclonal antibodies.
abstract: We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern was similar in bone marrow cells. In vitro differentiation of CD34+ progenitor cells induced BLT1 expression within 7 days, which remained constant up to day 17 when a further increase was measured and maintained up to day 20. BLT1 expression was modified by inflammatory mediators: LPS, TNFalpha, fMLP, as well as LTB4 itself, caused a slight down-regulation at 30 min, an effect that was particularly marked with PMA, whereas the effect was least pronounced with IL-8. The antibodies have proved to be useful in an extensive mapping of BLT1 in both peripheral blood and bone marrow and as a tool to elucidate changes in the receptor expression.
authors.affiliation: Division of Molecular Neurobiology, Wallenberg Neuroscience Center, BMC A12, Lund University, 22184 Lund, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Pettersson
authors.forename: Johan
authors.initials: J
authors.lastname: Richter
authors.forename: Christer
authors.initials: C
authors.lastname: Owman
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D018102:Receptors, Leukotriene B4 / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D018102:Receptors, Leukotriene B4