title: Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus.
abstract: Backfat thickness is one of the major quantitative traits that affect carcass quality in beef cattle. In this study, we have fine mapped a QTL for backfat EBV on bovine chromosome 14, using an identical-by-descent haplotype-sharing analysis, in a commercial line of Bos taurus. We also examined the association between gene-specific single nucleotide polymorphism (SNP) markers of the genes diacylgcerol acyltransferase 1 (DGAT1) and thyroglobulin (TG) and the backfat EBV. The results indicate that the QTL region for backfat identified on chromosome 14 is in agreement with previous studies. However, neither of the two polymorphisms of candidate genes tested, DGAT1 nor TG, showed a significant (P > 0.10) association with the backfat EBV in the cattle populations examined. However, a strong association (P = 0.0058) was detected between a microsatellite marker (CSSM66) lying approximately mid-way between the two candidate genes and the backfat EBV. These results suggest that other SNP of DGAT1, TG, or other gene(s) in the chromosomal region should be examined to test whether they have a significant effect on lipid metabolism.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. stephen.moore@ualberta.ca
authors.forename: S S
authors.initials: SS
authors.lastname: Moore
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Basarab
authors.forename: W M
authors.initials: WM
authors.lastname: Snelling
authors.forename: J
authors.initials: J
authors.lastname: Kneeland
authors.forename: B
authors.initials: B
authors.lastname: Murdoch
authors.forename: C
authors.initials: C
authors.lastname: Hansen
authors.forename: B
authors.initials: B
authors.lastname: Benkel
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000273:Adipose Tissue / Q000033:anatomy & histology* / Q000254:growth & development; D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D051048:Diacylglycerol O-Acyltransferase; D005838:Genotype; D006239:Haplotypes; D008297:Male; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D013954:Thyroglobulin / Q000235:genetics*
chemical_list: D013954:Thyroglobulin; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase