Identification and characterization of p100HB, a new mutant form of p100/NF-kappa B2.
P100, which is encoded by NF-kappa B2, inhibits Rel dimers. It can also be processed into p52, one of the DNA binding sub-units of NF-kappa B/Rel factors. Several p100 C-terminal truncations that result from gene rearrangements are associated with lymphomagenesis. Here, we characterized a new p100 mutant that we termed p100HB. It originates from a point-mutation that generates a premature stop-codon, and thus the protein lacks the last 125 amino acids. We have detected p100HB in several human tumor cell lines. The truncated protein is mainly unprocessed, and although it still binds Rel dimers, it has reduced inhibitory potency compared to p100 and translocates into the nucleus. Thus, p100HB may be associated with deregulated NF-kappa B/Rel functions.
Derudder
Emmanuel
E
Oncogenèse, Différenciation et Transduction du Signal, UPR 9079 du CNRS, Institut André Lwoff,7, rue Guy Môquet, 94801 Villejuif, France.
Laferté
Arnaud
A
Ferreira
Valérie
V
Mishal
Zohair
Z
Baud
Véronique
V
Tarantino
Nadine
N
Körner
Marie
M
D021581:Active Transport, Cell Nucleus; D048868:Adaptor Proteins, Signal Transducing; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry; D003062:Codon; D019281:Dimerization; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D019169:Jurkat Cells; D008214:Lymphocytes / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D016328:NF-kappa B / Q000737:chemistry* / Q000235:genetics*; D052003:NF-kappa B p52 Subunit; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003062:Codon; D020672:I-kappa B Proteins; D016328:NF-kappa B; D052003:NF-kappa B p52 Subunit; D012333:RNA, Messenger; C110409:THAP12 protein, human