Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells.
Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After proliferation of undifferentiated ES-like cells in vitro, some bovine ES-like cells differentiated to neural precursor cells, which were cultured in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet-derived growth factor (PDGF). In addition, calves were successfully cloned using ES-like cells and the frequency of term pregnancies for blastocysts derived from ES-like cells was higher than those of early pregnancies and maintained pregnancies after nuclear transplantation (NT) with bovine somatic cells. Successful cloning from bovine ES-like cells should allow the introduction into cattle of specific genetic characteristics of biomedical and/or agricultural importance.
Saito
Shigeo
S
Saito Laboratory of Cell Technology, Yaita, Tochigi, Japan.
Sawai
Ken
K
Ugai
Hideyo
H
Moriyasu
Satoru
S
Minamihashi
Akira
A
Yamamoto
Yusuke
Y
Hirayama
Hiroki
H
Kageyama
Soichi
S
Pan
Jianzhi
J
Murata
Takehide
T
Kobayashi
Yoshiro
Y
Obata
Yuichi
Y
Yokoyama
Kazunari K
KK
D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D019976:Cloning, Organism / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D019076:Transgenes
D008164:Luminescent Proteins; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins; D004815:Epidermal Growth Factor