title: Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice.
abstract: Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
authors.forename: Valéria Rêgo Alves
authors.initials: VR
authors.lastname: Pereira
authors.forename: Virginia Maria Barros
authors.initials: VM
authors.lastname: de Lorena
authors.forename: Mineo
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Ana Paula Galvão
authors.initials: AP
authors.lastname: da Silva
authors.forename: Ulisses
authors.initials: U
authors.lastname: Montarroyos
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Correa-Oliveira
authors.forename: Yara de Miranda
authors.initials: Yde M
authors.lastname: Gomes
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000941:Antigens; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; C063362:QF116 antigen, Plasmodium falciparum; C023456:fibrinogen-related antigen