[Glucose uptake of activated platelets and the influence by integrin antibodies].
OBJECTIVE
To study the signal transduction in the course of energy metabolism of platelets, and to provide a new theoretical basis for clinical application of the anti-activation of platelets by the monoantibody of integrin GPIIb/IIIa.


METHODS
With or without the block of integrin GPIIb/IIIa monoantibody 10E5 and/or integrin alpha v beta 3 monoantibody 609, the uptake rate of tritium-labeled 2-deoxyglucose([3H]2-DG) by platelets activated by thrombin was counted in a liquid scintillation counter. The relationship between the energy metabolism of platelets and platelet integrin was investigated by the course of glucose uptake of individual vivo platelets.


RESULTS
Compared with unactivated platelets, the glucose uptake of activated platelets increased, and it could be blocked by monoantibody 10E5 and partly blocked by 609.


CONCLUSIONS
The glucose uptake of activated platelets can be suppressed by monoantibody 10E5, and the intergrin family is associated with the signal transduction of platelet energy metabolism. Integrin GPIIb/IIIa may play an important role in the procedure of platelet energy metabolism.
Fu
Gan
G
Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
Liu
Wei
W
Chen
Fang-ping
FP
D001786:Blood Glucose / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D015539:Platelet Activation / Q000502:physiology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000502:physiology*; D015398:Signal Transduction
D001786:Blood Glucose; D019039:Platelet Glycoprotein GPIIb-IIIa Complex