title: Interference-free analysis using three-way fluorescence data and the parallel factor model. Determination of fluoroquinolone antibiotics in human serum.
abstract: Three-way fluorescence data and multivariate calibration based on parallel factor analysis (PARAFAC) are combined for the simultaneous quantitation of three fluoroquinolone anitibiotics (norfloxacin, enoxacin, and ofloxacin) in human serum samples. The three analytes can be adequately determined with limits of detection of 0.2, 3.0, and 0.5 microg L(-1), respectively, with minimum experimental effort. The selected analytical methodology fully exploits the so-called second-order advantage of the employed three-way data, allowing obtaining individual concentrations of calibrated analytes in the presence of any number of uncalibrated (serum) components. In contrast to PARAFAC, less satisfactory results were obtained with a multidimensional partial least-squares (nPLS) model trained with the same calibration set.
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, España.
authors.forename: Arsenio
authors.initials: A
authors.lastname: Muñoz de la Peña
authors.forename: Anunciación
authors.initials: A
authors.lastname: Espinosa Mansilla
authors.forename: David
authors.initials: D
authors.lastname: González Gómez
authors.forename: Alejandro C
authors.initials: AC
authors.lastname: Olivieri
authors.forename: Héctor C
authors.initials: HC
authors.lastname: Goicoechea
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood*; D002138:Calibration; D003627:Data Interpretation, Statistical; D024841:Fluoroquinolones / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D015999:Multivariate Analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones