Chronic treatment with imipramine inhibits cell growth and enhances serotonin 2C receptor mRNA expression in NG 108-15 cells.
We previously reported that NG108-15 cells contain intrinsic serotonin 2C receptor (5-HT2CR). The effects of imipramine, a 5-HT2CR antagonist, on cell growth, cell viability, and the 5-HT2CR mRNA level were investigated in this study. Repeated treatment with imipramine at concentrations of 1 - 10 microM for 5 days inhibited cell growth in a concentration-dependent manner without affecting cell viability. In addition, the level of 5-HT2CR mRNA was elevated. At 30 microM, imipramine significantly reduced cell viability. Our findings suggest that the effect of imipramine on neuronal growth may be related to its effects on 5-HT2CR.
Sukma
Monrudee
M
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Tohda
Michihisa
M
Watanabe
Hiroshi
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D007099:Imipramine / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D044405:Receptor, Serotonin, 5-HT2C / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D044405:Receptor, Serotonin, 5-HT2C; D007099:Imipramine