title: Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms.
abstract: The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examined tools used for gene cloning and functional studies of DNA repair in eukaryotes, using this bacterial system as a model.
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, 30161-970 Belo Horizonte, MG, Brasil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Augusto-Pinto
authors.forename: Carlos Gustavo Regis
authors.initials: CG
authors.lastname: da Silva
authors.forename: Débora de Oliveira
authors.initials: Dde O
authors.lastname: Lopes
authors.forename: Alice
authors.initials: A
authors.lastname: Machado-Silva
authors.forename: Carlos Renato
authors.initials: CR
authors.lastname: Machado
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005057:Eukaryotic Cells; D005798:Genes, Bacterial; D008957:Models, Genetic