title: Mitochondrial sources of H2O2 generation play a key role in flow-mediated dilation in human coronary resistance arteries.
abstract: We previously showed that hydrogen peroxide (H2O2) contributes to flow-induced dilation in human coronary resistance arteries (HCRAs); however, the source of this H2O2 is not known. We hypothesized that the H2O2 is derived from superoxide (O2*-) generated by mitochondrial respiration. HCRAs were dissected from right atrial appendages obtained from patients during cardiac surgery and cannulated with micropipettes. H2O2-derived radicals and O2*- were detected by electron spin resonance (ESR) using BMPO as the spin trap and by histofluorescence using hydroethidine (HE, 5 micromol/L) and dichlorodihydrofluorescein (DCFH, 5 micromol/L). Diameter changes to increases in pressure gradients (20 and 100 cm H2O) were examined in the absence and the presence of rotenone (1 micromol/L), myxothiazol (100 nmol/L), cyanide (1 micromol/L), mitochondrial complex I, III, and IV inhibitors, respectively, and apocynin (3 mmol/L), a NADPH oxidase inhibitor. At a pressure gradient of 100 cm H2O, ubisemiquinone and hydroxyl radicals were detected from effluents of vessels. Including superoxide dismutase and catalase in the perfusate reduced the ESR signals. Relative ethidium and DCFH fluorescence intensities in HCRAs exposed to flow were enhanced (1.45+/-0.15 and 1.57+/-0.12, respectively compared with no-flow) and were inhibited by rotenone (0.87+/-0.17 and 0.95+/-0.07). Videomicroscopic studies showed that rotenone and myxothiazol blocked flow-induced dilation (% max. dilation at 100 cm H2O: rotenone, 74+/-3% versus 3+/-13%; myxothiazol, 67+/-3% versus 28+/-4%; P<0.05). Neither cyanide nor apocynin altered flow-induced dilation. These results suggest that shear stress induced H2O2 formation, and flow-induced dilation is derived from O2*- originating from mitochondrial respiration.
authors.affiliation: Department of Internal Medicine, Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, Wis 53226, USA. ypliu@mcw.edu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhao
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Kalyanaraman
authors.forename: Alfred C
authors.initials: AC
authors.lastname: Nicolosi
authors.forename: David D
authors.initials: DD
authors.lastname: Gutterman
mesh_terms: D003326:Coronary Circulation; D003331:Coronary Vessels / Q000378:metabolism / Q000502:physiology*; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006331:Heart Diseases / Q000503:physiopathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D008689:Methacrylates; D008833:Microcirculation; D008875:Middle Aged; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D019255:NADPH Oxidases / Q000502:physiology; D012402:Rotenone / Q000494:pharmacology; D013314:Stress, Mechanical; D013844:Thiazoles / Q000494:pharmacology; D014655:Vascular Resistance; D014664:Vasodilation / Q000187:drug effects
chemical_list: D003486:Cyanides; D005609:Free Radicals; D008689:Methacrylates; D013844:Thiazoles; D012402:Rotenone; C030517:myxothiazol; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases