title: Purification, crystallization and preliminary diffraction studies of the Pseudomonas aeruginosa strain K122-4 monomeric pilin.
abstract: The monomeric pilin from Pseudomonas aeruginosa strain K122-4 has been crystallized and preliminary X-ray diffraction data have been collected. Pilin is the monomeric subunit of the type IV pilus, the dominant adhesin of the opportunistic pathogen P. aeruginosa. The K122-4 pilin crystallizes as a dimer in space group P1, with unit-cell parameters a = 40.19, b = 38.93, c = 37.22 A, alpha = 66.38, beta = 111.12, gamma = 93.74 degrees. Diffraction data were collected using a synchrotron-radiation source and were processed to 1.54 A d-spacing.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Audette
authors.forename: Randall T
authors.initials: RT
authors.lastname: Irvin
authors.forename: Bart
authors.initials: B
authors.lastname: Hazes
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods*; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010861:Fimbriae, Bacterial / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000648:ultrastructure; D014961:X-Ray Diffraction
chemical_list: D018829:Adhesins, Bacterial; D036781:Fimbriae Proteins