title: Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and northeastern Colombia.
abstract: Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (F ST: 0.035; Nm: 6.8) but lower between these two and the northeastern population (F ST: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (phi ST 0.1131, P < 0.001). The variation among individuals within populations (phi ST 0.8869, P < 0.001)was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad del Valle, apartado Aéreo 25360, Cali, Colombia. ceposso@univalle.edu.co
authors.forename: Carmen Elisa
authors.initials: CE
authors.lastname: Posso
authors.forename: Ranulfo
authors.initials: R
authors.lastname: González
authors.forename: Heiber
authors.initials: H
authors.lastname: Cárdenas
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gallego
authors.forename: Myriam Cristina
authors.initials: MC
authors.lastname: Duque
authors.forename: Marco Fidel
authors.initials: MF
authors.lastname: Suarez
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D003105:Colombia; D017344:Genes, Insect / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D006579:Heterozygote; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers