[The role of supplementation or inhibition of nitric oxide production in burn injury to reduce ischemic damage].
BACKGROUND
To determine the role of nitric oxide (NO) which is vasodilator agent and inflammatory cytokine, in burn injury.


METHODS
Rats were divided into 5 groups, and a 30 % burn was inflicted. In addition to sham control and burn control groups, other 3 groups were given L-Arginine, and L-nitro-L-Arginine methylester (L-NAME), and both. Neutrophil and hematocrit percentage in blood, NO, TNF-alpha and malondialdehyde (MDA) levels in plasma and neutrophil infiltration in the lung were evaluated at 24 hours after thermal injury.


RESULTS
The inhibition of NO production with L-NAME treatment significantly decreased these parameters when compared to burned control group. MDA was decreased significantly in all groups which were given drugs.


CONCLUSIONS
The induction and inhibition of NO production both reduced lipid peroxidation but induction increased the mortality, plasma TNF-alpha and neutrophil in the blood. Inhibition of NO production is found more useful after thermal injury in rats.
Zilan
Aydin
A
Genel Cerrahi Uzmani, Istanbul, Büyuksehir Belediyesi, Sağlik Işleri Müdürlüğü, Sehzadebaşi Tip Merkezi.
Cetinkale
Oğuz
O
Kiran
Bayram
B
Unlüçerçi
Yeşim
Y
Olgaç
Vakur
V
Deniz
Günnur
G
Bekpinar
Selda
S
D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000627:therapeutic use*; D002056:Burns / Q000378:metabolism*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D007511:Ischemia / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000627:therapeutic use*; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004791:Enzyme Inhibitors; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester