Molecular characterization of dsRNA segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, W370, of the plant pathogen Rosellinia necatrix.
A hypovirulent isolate, W370, of the white root rot fungus Rosellinia necatrix has previously been shown to harbour 12 dsRNA segments. In this study, complete nucleotide sequences of segments 2 and 5 of W370 dsRNAs were determined. The nucleotide sequence of genome segment 2 was 3773 bases long with a single long open reading frame (ORF) encoding 1226 amino acid residues with a predicted molecular mass of approximately 138.5 kDa. The nucleotide sequence of segment 5 was 2089 bases long with a single long ORF, whose deduced polypeptide contained 646 amino acid residues with a predicted molecular mass of about 72 kDa. Comparative analysis showed that the deduced protein sequence of segment 2 had significant homology with the putative VP2 of Colorado tick fever virus (CTFV) and European Eyach virus (EYAV) in the genus Coltivirus, but the deduced protein sequence of segment 5 had no similarity with other virus proteins. Double-shelled spherical particles approximately 80 nm in diameter associated with W370 dsRNAs were observed in a preparation from the mycelial tissue of isolate W370. The results demonstrated that the virus associated with W370 dsRNAs is a novel reovirus of the family Reoviridae. The virus was named Rosellinia anti-rot virus (RArV).
Wei
Chuan Zhao
CZ
National Institute of Fruit Tree Science, Fujimoto, Tsukuba 305-8605, Japan.
Osaki
Hideki
H
National Institute of Fruit Tree Science, Fujimoto, Tsukuba 305-8605, Japan.
Iwanami
Toru
T
National Institute of Fruit Tree Science, Fujimoto, Tsukuba 305-8605, Japan.
Matsumoto
Naoyuki
N
National Institute for Agro-Environmental Sciences, Kan-nondai, Tsukuba 305-8604, Japan.
Ohtsu
Yoshihiro
Y
National Institute of Fruit Tree Science, Fujimoto, Tsukuba 305-8605, Japan.
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000472:pathogenicity / Q000821:virology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D012330:RNA, Double-Stranded; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure*; D016415:Sequence Alignment; D009626:Terminology as Topic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins