title: Regulation of sustained actin dynamics by the TCR and costimulation as a mechanism of receptor localization.
abstract: The localization of receptors, signaling intermediates, and cytoskeletal components at the T cell/APC interface is thought to be a major determinant of efficient T cell activation. However, important questions remain open. What are the dynamics of the T cell cytoskeleton as a potential mediator of such localization? How are they regulated by the TCR and costimulatory receptors? Do they actually mediate receptor localization? In this study, we have addressed these questions. Even under limiting T cell activation conditions, actin accumulated immediately and transiently at the T cell/APC interface, the microtubule organizing center reoriented toward it. In contrast, sustained (>5 min) actin accumulation in highly dynamic patterns depended on an optimal T cell stimulus: high concentrations of the strong TCR ligand agonist peptide/MHC and engagement of the costimulatory receptors CD28 and LFA-1 were required in an overlapping, yet distinct, fashion. Intact sustained actin dynamics were required for interface accumulation of TCR/MHC in a central pattern and for efficient T cell proliferation, as established using a novel approach to selectively block only the sustained actin dynamics. These data suggest that control of specific elements of actin dynamics by TCR and costimulatory receptors is a mechanism to regulate the efficiency of T cell activation.
authors.affiliation: Center for Immunology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Tskvitaria-Fuller
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Rozelle
authors.forename: Helen L
authors.initials: HL
authors.lastname: Yin
authors.forename: Christoph
authors.initials: C
authors.lastname: Wülfing
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D002450:Cell Communication / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006649:Histocompatibility Antigens / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D022101:Microtubule-Organizing Center / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D011554:Pseudopodia / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics
chemical_list: D000199:Actins; D018106:CD28 Antigens; D006649:Histocompatibility Antigens; D008164:Luminescent Proteins; D016169:Lymphocyte Function-Associated Antigen-1; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins