Antioxidant constituents from the stem of Sorghum bicolor.
The EtOAc soluble fraction from the stem of Sorghum bicolor showed a strong free radical scavenging activity. Five major compounds were isolated from this fraction. They were identified by spectral data as methyl ferulate (1), methyl p-hydroxycinnamate (2), p-hydroxybenzaldehyde (3), tricin (4), and quercetin 3,4'-dimethyl ether (5). Among these compounds, 1 exhibited a strong, free radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) with an IC50 value of 0.7 microM. We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. All five compounds showed anti-lipid peroxidation activity (IC50 values of 0.5, 0.4, 0.3 and 0.3 microM, respectively).
Kwon
Yong Soo
YS
College of Pharmacy, Kangwon National University, 200-701, Korea.
Kim
Chang Min
CM
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley; D045868:Sorghum
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts