Molecular and functional characterization of the first nucleobase transporter gene from African trypanosomes.
African trypanosomes are unable to synthesize purines and depend upon purine nucleoside and nucleobase transporters to salvage these compounds from their hosts. To understand the crucial role of purine salvage in the survival of these parasites, a central objective is to identify and characterize all of the purine permeases that mediate uptake of these essential nutrients. We have cloned and functionally expressed in a purine nucleobase transport deficient strain of Saccharomyces cerevisiae a novel nucleobase transporter gene, TbNT8.1, from Trypanosoma brucei. The permease encoded by this gene mediates the uptake of hypoxanthine, adenine, guanine, and xanthine with Kms in the low micromolar range. The TbNT8.1 protein is a member of the equilibrative nucleoside transporter (ENT) family of permeases that occur in organisms as diverse as protozoa and mammals. TbNT8.1 is distinct from other ENT permeases that have been identified in trypanosomes in utilizing multiple purine nucleobases, rather than purine nucleosides, as substrates and is hence the first bona fide nucleobase permease identified in these parasites. Furthermore, unlike the mRNAs for other purine transporters, TbNT8.1 mRNA is significantly more abundant in insect stage procyclic forms than in mammalian stage bloodstream forms, and the TbNT8.1 permease thus may represent a major route for purine nucleobase uptake in procyclic trypanosomes.
Henriques
Cristina
C
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201, USA.
Sanchez
Marco A
MA
Tryon
Rob
R
Landfear
Scott M
SM
D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D005786:Gene Expression Regulation; D007700:Kinetics; D008969:Molecular Sequence Data; D033702:Nucleobase Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009865:Oocytes; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014981:Xenopus
D016054:DNA, Protozoan; D033702:Nucleobase Transport Proteins; D015800:Protozoan Proteins; D011687:Purines; D011994:Recombinant Proteins