Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms.
The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examined tools used for gene cloning and functional studies of DNA repair in eukaryotes, using this bacterial system as a model.
Augusto-Pinto
Luiz
L
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, 30161-970 Belo Horizonte, MG, Brasil.
da Silva
Carlos Gustavo Regis
CG
Lopes
Débora de Oliveira
Dde O
Machado-Silva
Alice
A
Machado
Carlos Renato
CR
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005057:Eukaryotic Cells; D005798:Genes, Bacterial; D008957:Models, Genetic