Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pylori.
The triple-base-pair 16S rDNA mutation AGA(926-928)-->TTC mediates high-level tetracycline resistance in Helicobacter pylori. In contrast, single- and double-base-pair mutations mediated only low-level tetracycline resistance and decreased growth rates in the presence of tetracycline, explaining the preference for the TTC mutation in tetracycline-resistant H. pylori isolates.
Gerrits
Monique M
MM
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands.
Berning
Marco
M
Van Vliet
Arnoud H M
AH
Kuipers
Ernst J
EJ
Kusters
Johannes G
JG
D003001:Cloning, Molecular; D017931:DNA Primers; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics* / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013753:Tetracycline Resistance / Q000235:genetics* / Q000502:physiology*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S