Comparison of structure-activity relations of corazonin using two different bioassay systems.
The structure-activity relations of [His(7)]-corazonin were studied using two different bioassay systems; i.e. inhibitory effect on spinning rate in the silkworm, Bombyx mori, and darkening response in albino nymphs of the migratory locust, Locusta migratoria. Deletion of the N-terminus, shortening of the peptide and single amino acid substitutions reduced activity in a similar manner except for the minimum effective dose in the two insects. The results also revealed that the residues at position 1, 3 and 5 were particularly important for biological activity. Despite the different physiological affects, the two insect species exhibited similar structure-activity relationships, suggesting that they might have similar receptor systems.
Tanaka
Y
Y
Laboratory of Insect Growth Regulation, National Institute of Agrobiological Science, Tsukuba, Ibaraki 305-8634, Japan. yoshiaki@affrc.go.jp
Ishibashi
J
J
Tanaka
S
S
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001120:Arginine; D001681:Biological Assay; D012831:Bombyx / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006110:Grasshoppers / Q000187:drug effects* / Q000502:physiology; D006639:Histidine; D007301:Insect Hormones / Q000494:pharmacology; D019476:Insect Proteins; D007814:Larva; D008297:Male; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000378:metabolism / Q000031:analogs & derivatives; D013329:Structure-Activity Relationship
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; D009842:Oligopeptides; C044833:adipokinetic hormone; C060602:corazonin protein, insect; D006639:Histidine; C014956:adipokinetic hormone (locust); D001120:Arginine; D011761:Pyrrolidonecarboxylic Acid