A sensitive method for detection of sulfamethazine and N4-acetylsulfamethazine residues in environmental samples using solid phase immunoextraction coupled with MALDI-TOF MS.
Sulfamethazine (SMT) and its major metabolite, N(4)-acetylsulfamethazine (NA-SMT), were each recovered from spiked water (0.1 ppb) and 10% (w/v) aqueous suspensions of soil (1 ppb) or composted manure (1 ppb), by using a three-stage solid phase immunoextraction (SPIE) system, followed by detection with matrix-assisted laser/desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Sulfonamide recovery rates are reported for separate stages of the SPIE system and for trace-level sulfonamide SPIE extraction from the environmental samples. SPIE MALDI-TOF MS is a rapid and definitive technique with potentially better efficiency relative to other established trace-level sulfonamide analytical methods. SPIE MALDI-TOF MS required 1.5 h per batch (8-24 samples/batch) for sample enrichment, 5 min per batch for probe preparation, and 5 min per sample to acquire and process the spectrum. This is the first time MALDI-TOF MS has been reported as a potential means of detecting trace-level drug residues in complex environmental samples.
Grant
G A
GA
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Frison
S L
SL
Sporns
P
P
D000890:Anti-Infective Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis*; D008372:Manure / Q000032:analysis; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013418:Sulfamethazine / Q000031:analogs & derivatives / Q000032:analysis*; D014867:Water / Q000032:analysis
D000890:Anti-Infective Agents; D004785:Environmental Pollutants; D008372:Manure; D012987:Soil; D014867:Water; C477731:N(4)-acetylsulfamethazine; D013418:Sulfamethazine