title: Novel spastin mutations and their expression analysis in two Italian families.
abstract: Mutations in spastin cause the most common form of pure autosomal dominant hereditary spastic paraparesis (SPG4). Here, we report two Italian families affected with SPG4-linked HSP harboring two novel spastin mutations. SSCP/sequencing analysis of the spastin gene showed a single base pair deletion causing a frame-shift in one family (1442delT) and a missense mutation (1726T>C) resulting in a leucine to proline amino-acid change (L534P) in the other family. Total RNA from the mutant and the wild-type spastin allele in muscle biopsies from patients from the two affected families was quantitated. RNA expression was almost absent from the spastin allele harboring the single base pair deletion, while it was nearly normal for the spastin allele harboring the missense mutation. These data suggest that varying spastin RNA levels are found in out-of-frame and missense spastin mutations and imply different mechanisms involved in the molecular pathology of SPG4 linked HSP.
authors.affiliation: Department of Neurosciences, University of Padua, via Giustiniani 5, 35128 Padua, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Molon
authors.forename: Pasquale
authors.initials: P
authors.lastname: Montagna
authors.forename: Corrado
authors.initials: C
authors.lastname: Angelini
authors.forename: Elena
authors.initials: E
authors.lastname: Pegoraro
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000483:Alleles; D004252:DNA Mutational Analysis; D017931:DNA Primers; D016368:Frameshift Mutation / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007558:Italy; D007797:Laminin; D020125:Mutation, Missense / Q000235:genetics; D020336:Paraparesis, Spastic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000074181:Spastin
chemical_list: D017931:DNA Primers; D007797:Laminin; C102645:laminin alpha 2; D000251:Adenosine Triphosphatases; D000074181:Spastin; C400283:SPAST protein, human