Effect of native phosphocaseinate on the in vitro preservation of fresh semen.
The fertilization capacity of goat sperm stored in milk extenders is approximately 12-24h. Long-term storage of goat sperm (up to 3 days) is desirable as it would confer greater flexibility to breeding farms. The aim of this study was to evaluate in vitro motility parameters of buck spermatozoa for up to 7 days of storage using skim milk or chemically defined extender supplemented with native phosphocaseinate (NPPC). Four experiments were conducted to determine optimum temperature (4 or 15 degrees C) and storage conditions (aerobic versus anaerobic), the effect of seminal plasma on sperm survival, the optimal concentration of NPPC and the effect of beta lactoglobulin (BL). Both skim milk and NPPC were found to be more efficient for preserving goat sperm at 4 degrees C than at 15 degrees C (P<0.01). Furthermore, when sperm was stored at 4 degrees C, no detrimental effects of seminal plasma were observed. Our results showed that motility parameters can be maintained with success until Day 4. However, NPPC-based extenders extend the in vitro survival to 7 days of storage. The optimal concentration of NPPC for the preservation of sperm cells for 4 days of storage was 81g/l and for 7 days of storage was 81 and 54g/l. No effect of the supplementation of the NPPC extender with BL was found.
Leboeuf
B
B
Unité Expérimentale d'Insémination Artificielle, INRA, 86480 Rouillé, France. leboeuf@lusignan.inra.fr
Guillouet
Ph
P
Batellier
F
F
Bernelas
D
D
Bonné
J L
JL
Forgerit
Y
Y
Renaud
G
G
Magistrini
M
M
D000818:Animals; D002470:Cell Survival; D006041:Goats; D007782:Lactoglobulins / Q000008:administration & dosage; D008297:Male; D008892:Milk / Q000737:chemistry*; D010100:Oxygen / Q000008:administration & dosage; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D007782:Lactoglobulins; D010100:Oxygen