title: Intracytoplasmic lumina in medullary carcinoma of the thyroid gland. Report of a case with cytologic and immunocytochemical features.
abstract: BACKGROUND
  Intracytoplasmic lumina (ICL) have been observed frequently in breast carcinoma cells. However, they are extremely rare in thyroid gland tumors. We encountered a medullary carcinoma of the thyroid (MCT) with ICL and present a case with cytologic, immunocytochemical and ultrastructural features.
  
  
  METHODS
  A 15-year-old female was admitted with a left thyroid mass. Ultrasound examination revealed a well-defined tumor in the left lobe of the thyroid. Fine needle aspiration cytology showed mainly dispersed spindle cells with oval nuclei and some polymorphic or triangular tumor cells. The tumor cells containing ICL were noted at high magnification. The ICL contained sparse microvilli and abundant granular material with dense, round bodies on ultrastructural sections. Immunocytochemically, these tumor cells were positive for calcitonin and carcinoembryonic antigen (CEA). Moreover, CEA was recognized in the ICL with immunocytochemical staining. All tumor cells were negative for thyroglobulin. Pathologic examination confirmed the diagnosis of medullary carcinoma of the thyroid gland.
  
  
  CONCLUSIONS
  MCT can include ICL with granular material containing CEA.
authors.affiliation: Department of Pathology, Okinawa Prefectural Chubu Hospital, 208-3, Miyazato, Gushikawa City, Okinawa, 904-2293, Japan.
authors.forename: Mitsuyuki
authors.initials: M
authors.lastname: Kinjo
authors.forename: Cyourei
authors.initials: C
authors.lastname: Yohena
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Kunishima
mesh_terms: D000293:Adolescent; D002116:Calcitonin / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D018276:Carcinoma, Medullary / Q000000981:diagnostic imaging / Q000473:pathology*; D003593:Cytoplasm / Q000473:pathology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013961:Thyroid Gland / Q000473:pathology* / Q000648:ultrastructure; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D014463:Ultrasonography
chemical_list: D002272:Carcinoembryonic Antigen; D002116:Calcitonin