Validation of an HPLC method for the determination of ciprofloxacin in human plasma.
A simple HPLC method with fluorescence detection of ciprofloxacin in human plasma was developed and validated. After protein precipitation, chromatographic separation of ciprofloxacin in plasma was achieved at 35 degrees C with a C18 column and acetonitrile-phosphate mixture, pH 3, as mobile phase. Quantitative determination was performed by fluorimetry after excitation at 278 nm. The method was specific and validated with a limit of quantification of 41 ng/ml. The intra- and inter-day coefficients of variation were between 0.5 and 6.6% and accuracy between -2.02 and 7.04%. Ciprofloxacin was stable in plasma for 40 days at -20 degrees C and after three freezing-thawing cycles. The method has been applied in a bioequivalence study of two formulation of 500 mg ciprofloxacin.
Imre
Silvia
S
Faculty of Pharmacy, University of Medicine and Pharmacy, Gh. Marinescu Street, 38, Tg. Mures 4300, Romania. silsta@yahoo.com
Dogaru
Maria T
MT
Vari
C E
CE
Muntean
T
T
Kelemen
L
L
D000893:Anti-Inflammatory Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000097:blood*; D005470:Fluorometry; D005615:Freezing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013048:Specimen Handling; D013810:Therapeutic Equivalency
D000893:Anti-Inflammatory Agents; D007202:Indicators and Reagents; D002939:Ciprofloxacin