[Significative lengthening of the time of cornea conservation supporting its natural tension and curvature].
The success of cornea transplantations has always been a concern of world researchers to identify risk factors from the proper surgical techniques up to the several tissue storage procedures, such as the ones used in the new laboratories called eyes banks. However, the integral research works are still limited at present and they continue putting emphasis in the donor cornea storage that guarantee up to for this tissue feasibility. This research had the aim to contribute to the improvement of the corneal tissue quality taking into account the maintenance of its natural curvature and tensions combining them with some standard preservation solutions. This was achieved by means of a new mechanical system. Assays with rabbits were designed according to a sequential experimental groups design. In these ones, the study of the feasibility parameters behavior under different conservation media, related to the time, was splitted into four blocks. The first and second ones attended the cornea conservation in the short and intermediate-terms culture mediums as the ones at 4 degrees C degree isolated cornea preservation and the usual ones such as the Mc Carey Kaufman, Optisol and Likorol solutions used in the eyes banks. The third one was designed for the surgical assays coming from the two named groups and the last one focused the time study of these parameters for a new conservation technique, developed as an alternative medium to keep the cornea tissue curvatures and tensions as well as its nutrition and hydration. The results showed that tissue natural tensions maintenance is an significant factor in the conservation process, which involve the cornea feasibility for the its use in future transplantations. Since the new developed technique is very simple, this study is considered to be a significant contribution for the storage and biological material handling steps.
Maffrand
R A
RA
Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Rep. Argentina. rmaffrand@cordoba.com.ar
P de Fabro
S
S
D000818:Animals; D003315:Cornea; D016039:Corneal Transplantation; D009926:Organ Preservation / Q000379:methods*; D011817:Rabbits; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D014022:Tissue Survival / Q000502:physiology