title: Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry.
abstract: A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 microl trifluoroacetic anhydride (TFAA), 60 degrees C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-AFNZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M(+)), m/z 350, 351, 360, 378, 379(M(+)), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.
authors.affiliation: Course of Social Medicine, Department of Legal Medicine, Osaka University Graduate School of Medicine F3, Suita-shi, Osaka 565-0871, Japan. terada@legal.med.osaka-u.ac.jp
authors.forename: Masaru
authors.initials: M
authors.lastname: Terada
authors.forename: Sousuke
authors.initials: S
authors.lastname: Masui
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Watanabe
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Inoue
authors.forename: Morio
authors.initials: M
authors.lastname: Iino
authors.forename: Masato
authors.initials: M
authors.lastname: Nakatome
authors.forename: Ryoji
authors.initials: R
authors.lastname: Matoba
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shinozuka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murai
authors.forename: Einosuke
authors.initials: E
authors.lastname: Tanaka
authors.forename: Katsuya
authors.initials: K
authors.lastname: Honda
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood* / Q000737:chemistry; D005445:Flunitrazepam / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D012680:Sensitivity and Specificity
chemical_list: D014151:Anti-Anxiety Agents; D005445:Flunitrazepam; C036343:7-aminoflunitrazepam