Mapping candidate hotspots of meiotic recombination in segments of human DNA cloned in the yeast Saccharomyces cerevisiae.
The hotspots of meiotic recombination in the human genome can be localized by genetic techniques. The resolution of these techniques is in the range of kilobases and depends on the density of the physical markers identifying allelic variants of the chromosomal loci. We thought it would be interesting to localize these sites with higher resolution. Assuming that some human chromosomal sites conserve their propensity for recombination when cloned in yeast, we localized the hotspots of recombination in several yeast artificial chromosomes (YACs) carrying human DNA. A number of potential recombination hotspots could be identified in the clones studied. Among them there are two classes of sites that are particularly recombination prone also in human meiotic cells: sites associated with CpG islands and sites located in the vicinity of long minisatellite sequences.
Mucha
M
M
Laboratoire de Biochimie de la Chromatine, Institut J. Monod, Université Paris VI et Paris VII, 2, Place Jussieu Tour 43, 75251, Paris, France.
Król
J
J
Goc
A
A
Filipski
J
J
D001483:Base Sequence; D019457:Chromosome Breakage; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular; D018899:CpG Islands; D004274:DNA, Recombinant / Q000235:genetics*; D003850:Deoxyribonuclease I; D004171:Diploidy; D015894:Genome, Human; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
D004274:DNA, Recombinant; D003850:Deoxyribonuclease I