Somata of nerve-injured sensory neurons exhibit enhanced responses to inflammatory mediators.
The effects of inflammatory mediators in modulating the activity of nerve-injured dorsal root ganglion (DRG) neurons were studied in rats in an in vitro nerve-DRG preparation 2-4 weeks after a loose ligation of the sciatic nerve (chronic constriction injury, CCI). An inflammatory soup (IS) of bradykinin, serotonin, prostaglandin E2 and histamine (each 10(-5) M, pH=7.4) was applied topically to the DRG. Evoked responses were recorded extracellularly from teased dorsal root fibers or intracellularly with sharp electrodes from somata of DRG neurons with myelinated (Abeta and Adelta) or unmyelinated (C) axons. IS increased the rate of ongoing spontaneous activity recorded from dorsal root fibers of CCI neurons and evoked activity in a subpopulation of previously 'silent' fibers in CCI rats but not those of unoperated controls. In comparison with DRG somata of control rats, those of CCI become more excitable as evidenced by a lower threshold to depolarizing current and a greater depolarization in response to IS. Inflammatory mediators, by increasing the excitability of DRG neurons, may contribute to paresthesiae, pain and hyperalgesia after peripheral nerve injury.
Song
Xue-Jun
XJ
Parker College Research Institute, Dallas, TX, USA Department of Anesthesiology, University of Arkansas for Medical Sciences, Little Rock, AR, USA The Fourth Military Medical University, Xi'an, People's Republic of China Department of Anesthesiology, Yale University School of Medicine, P.O. Box 208051, 333 Cedar Street, New Haven, CT 06520, USA.
Zhang
Jun-Ming
JM
Hu
San-Jue
SJ
LaMotte
Robert H
RH
D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000293:injuries / Q000502:physiology; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000494:pharmacology* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020426:Sciatic Neuropathy / Q000503:physiopathology*
D018836:Inflammation Mediators