Differential interleukin-8 and nitric oxide production in epithelial cells induced by mucosally invasive and noninvasive Trypanosoma cruzi trypomastigotes.
Trypanosoma cruzi metacyclic trypomastigotes (MT), but not blood form trypomastigotes (BFT), are highly mucosally infective. We investigated the abilities of MT and BFT to induce inflammation and/or intracellular killing activity within mucosal epithelia. BFT, but not MT, induced marked increases in interleukin-8, GRO-alpha, MCP-1, and nitric oxide production in HeLa and AGS cells, despite similar infectivities. MT may avoid induction of inflammation as an important biological mechanism facilitating mucosal invasion.
Eickhoff
C S
CS
Department of Internal Medicine, Saint Louis University Health Sciences Center, St. Louis, Missouri, USA.
Eckmann
L
L
Hoft
D F
DF
D000818:Animals; D002460:Cell Line; D014355:Chagas Disease / Q000209:etiology / Q000276:immunology; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054360:Chemokine CXCL1; D018925:Chemokines / Q000096:biosynthesis; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D009092:Mucous Membrane / Q000276:immunology / Q000469:parasitology; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology
C475829:CXCL1 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D012333:RNA, Messenger; D009569:Nitric Oxide