title: Genotoxicity, recombinogenicity and cellular preneoplasic transformation induced by vitamin A supplementation.
abstract: In spite of being one of the first vitamins to be discovered, the full range of biological activities of Vitamin A remains incomplete. A growing body of evidence has demonstrated an apparent enhancement of carcinogenesis, induced by dietary retinol. Since DNA damage is a well-recognized inducer of carcinogenesis, the aim of this study was to test the possible genotoxic effect of dietary retinol, using different types of bioassays. Retinol caused an increased recombinogenic activity in Drosophila melanogaster larvae as measured by the SMART test. In mammalian cell cultures, retinol supplementation-induced DNA double-strands breaks (DSB) and single-strands breaks (SSB), cell cycle progression and proliferative focus formation in terminal-differentiated rat Sertoli cells and increased DNA fragmentation in Chinese hamster lung fibroblasts (V79 cells), as measured by the comet assay. Altogether, our results suggest that retinol causes DNA damage and chromosomal rearrangements, which may disturbs key physiological processes and lead to cell cycle progression and preneoplasic transformation of terminal-differentiated mammalian cells.
authors.affiliation: Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, ICBS-Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2600 annexo, CEP, Porto Alegre, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Klamt
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
authors.forename: Rafael
authors.initials: R
authors.lastname: Roehrs
authors.forename: Ramatis Birnfeld
authors.initials: RB
authors.lastname: de Oliveira
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Dalmolin
authors.forename: João A P
authors.initials: JA
authors.lastname: Henriques
authors.forename: Heloisa Helena Rodrigues
authors.initials: HH
authors.lastname: de Andrades
authors.forename: Ana Lígia Lia
authors.initials: AL
authors.lastname: de Paula Ramos
authors.forename: Jenifer
authors.initials: J
authors.lastname: Saffi
authors.forename: José Cláudio Fonseca
authors.initials: JC
authors.lastname: Moreira
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation; D004260:DNA Repair; D019587:Dietary Supplements; D004331:Drosophila melanogaster / Q000235:genetics; D008297:Male; D009152:Mutagenicity Tests; D051381:Rats; D011995:Recombination, Genetic / Q000187:drug effects*; D014801:Vitamin A / Q000633:toxicity*
chemical_list: D014801:Vitamin A