title: High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology.
abstract: Isolation of intact RNA in high quality is the first and often the most critical step in performing many fundamental molecular biology experiments, and is essential for many techniques used in gene expression analysis. As many factors influence nucleic acid preservation, RNA isolation should include some important steps before and after the actual RNA extraction. We tested the influence of fixation and staining protocols regarding RNA integrity and concentration. A factor that is often underestimated is the absolute necessity for homogenous starting materials. Application of the LMPC technology allows for a rapidand highly precise procurement of purified cell populations suitable for a variety of downstream analyses.
authors.affiliation: P.A.L.M. Microlaser Technologies AG, Bernried, Germany. Renate.Burgemeister@palm-microlaser.com
authors.forename: Renate
authors.initials: R
authors.lastname: Burgemeister
authors.forename: Rainer
authors.initials: R
authors.lastname: Gangnus
authors.forename: Beate
authors.initials: B
authors.lastname: Haar
authors.forename: Karin
authors.initials: K
authors.lastname: Schütze
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sauer
mesh_terms: D000818:Animals; D004210:Dissection / Q000379:methods; D022781:Hepatocytes / Q000166:cytology; D016591:Histocytological Preparation Techniques / Q000379:methods; D007834:Lasers; D051379:Mice; D012313:RNA / Q000302:isolation & purification*
chemical_list: D012313:RNA