title: Functional cloning and characterization of a multidrug efflux pump, mexHI-opmD, from a Pseudomonas aeruginosa mutant.
abstract: We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiya
authors.forename: Takehiko
authors.initials: T
authors.lastname: Mima
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morita
authors.forename: Teruo
authors.initials: T
authors.lastname: Kuroda
authors.forename: Tohru
authors.initials: T
authors.lastname: Mizushima
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D003001:Cloning, Molecular; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D014639:Vanadium / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins; C477890:OpmD protein, Pseudomonas aeruginosa; D014639:Vanadium; D013752:Tetracycline