Hydrogen peroxide from the oxidative burst is not involved in the induction of taxol biosynthesis in Taxus chinensis cells.
In cell suspension cultures of Taxus chinensis, 40 mg/l fungal elicitor from Aspergillus niger and 20 microM HgCl2 elicited 5.7 and 3.6 mg/l taxol, which was a 9-fold and 5-fold increase vs. compared with the control, respectively. The fungal elicitor induced hydrogen peroxide (H2O2) accumulation but HgCl2 did not, indicating that H2O2 was not necessary for enhancement of taxol induced by elicitor. Compared with the treatment with fungal elicitor alone, exogenous catalase, ascorbic acid, diphenylene iodonium and superoxide dismutase induced a 0.45, 0.4, 0.7 and 1.4-fold H2O2, but elicited taxol production, which was 0.98, 1.2, 1.1 and 0.9-fold, respectively, vs. non-treated cells Elicitor-induced taxol production was not accorded with the amount of H2O2 production.
Lan
Wen Zhi
WZ
School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. lanwz73@sohu.com
Qin
Wen Min
WM
Yu
Long Jiang
LJ
Yang
Xi
X
D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis / Q000378:metabolism*; D001234:Aspergillus niger / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D017239:Paclitaxel / Q000138:chemical synthesis / Q000378:metabolism*; D016897:Respiratory Burst; D020946:Taxus / Q000382:microbiology / Q000502:physiology*
D000972:Antineoplastic Agents, Phytogenic; D006861:Hydrogen Peroxide; D017239:Paclitaxel