Spin-state selection in solid-state NMR.
Spin-state selection in solid-state NMR is demonstrated, using similar pulse sequences as used in liquid-state NMR. The different transitions of all three carbon resonances in fully 13C-labeled L-alanine are separated in different spectra. By selecting spin-states, the contribution of the J-coupling to the linewidth is removed, leading to a considerable enhancement in resolution. The spin-state-selective technique is demonstrated for magic-angle spinning frequencies from 6 to 35kHz. Other experimental conditions affecting the sensitivity of the experiments are discussed. Sensitivity losses due to the introduction of the spin-state-selective filter are shown to be acceptable. Finally, spin-state selection was used to experimentally confirm the differential broadening expected for the two transitions of the CH3 resonance.
Duma
Luminita
L
Laboratoire de Chimie (UMR-5532 CNRS/ENS), Laboratoire de Recherche Conventionné du CEA (23V), Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon, France.
Hediger
Sabine
S
Lesage
Anne
A
Emsley
Lyndon
L
D000409:Alanine / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry*; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
D002247:Carbon Isotopes; D013113:Spin Labels; D000409:Alanine