The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low).
The complete genome of Mycoplasma gallisepticum strain R(low) has been sequenced. The genome is composed of 996,422 bp with an overall G+C content of 31 mol%. It contains 742 putative coding DNA sequences (CDSs), representing a 91 % coding density. Function has been assigned to 469 of the CDSs, while 150 encode conserved hypothetical proteins and 123 remain as unique hypothetical proteins. The genome contains two copies of the rRNA genes and 33 tRNA genes. The origin of replication has been localized based on sequence analysis in the region of the dnaA gene. The vlhA family (previously termed pMGA) contains 43 genes distributed among five loci containing 8, 2, 9, 12 and 12 genes. This family of genes constitutes 10.4% (103 kb) of the total genome. Two CDSs were identified immediately downstream of gapA and crmA encoding proteins that share homology to cytadhesins GapA and CrmA. Based on motif analysis it is predicted that 80 genes encode lipoproteins and 149 proteins contain multiple transmembrane domains. The authors have identified 75 proteins putatively involved in transport of biomolecules, 12 transposases, and a number of potential virulence factors. The completion of this sequence has spawned multiple projects directed at defining the biological basis of M. gallisepticum.
Papazisi
Leka
L
Department of Pathobiology and Veterinary Science, The University of Connecticut, Storrs, CT 06269-3089, USA.|Center of Excellence for Vaccine Research, The University of Connecticut, Storrs, CT 06269-3089, USA.
Gorton
Timothy S
TS
Department of Pathobiology and Veterinary Science, The University of Connecticut, Storrs, CT 06269-3089, USA.|Center of Excellence for Vaccine Research, The University of Connecticut, Storrs, CT 06269-3089, USA.
Kutish
Gerald
G
Plum Island Animal Disease Center, US Department of Agriculture, Greenport, NY 11944, USA.
Markham
Philip F
PF
Department of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia.
Browning
Glenn F
GF
Department of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia.
Nguyen
Di Kim
DK
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Swartzell
Steven
S
Regulome Corporation, Seattle, WA 98195, USA.
Madan
Anup
A
The Institute for Systems Biology, Seattle, WA 98103-8904, USA.
Mahairas
Greg
G
Regulome Corporation, Seattle, WA 98195, USA.
Geary
Steven J
SJ
Department of Pathobiology and Veterinary Science, The University of Connecticut, Storrs, CT 06269-3089, USA.|Center of Excellence for Vaccine Research, The University of Connecticut, Storrs, CT 06269-3089, USA.
D000818:Animals; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D016366:Open Reading Frames / Q000235:genetics; D011200:Poultry / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D018741:Replication Origin / Q000235:genetics; D014774:Virulence / Q000235:genetics