[Analysis of cell components of CD59+ normal cells and abnormal clons in bone marrow of patients with paroxysmal nocturnal hemoglobinuria].
OBJECTIVE
To explore the cell components of normal cells and abnormal clons in bone marrow of patients with paroxysmal nocturnal hemoglobinuria (PNH).


METHODS
Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.


RESULTS
1. There was significant difference in BMNCs between normal CD59+ cells and abnormal clons (CD59-) cells, the normal CD59+ BMNCs were mainly composed of the cells in lymphocyte-window, while the abnormal CD59- BMNCs were mainly in blast window and in granulocyte-window. 2. The CD34+ cells in CD59+ BMNCs were also markedly decreased when compared with their CD59- counterparts, and the decrease of CD34+ cells in CD59+ BMNCs was more obvious in lymphocyte window than that in blast window.


CONCLUSIONS
The constituents of BMNCs and the amount of CD34+ cells in CD59+ and CD59- cell populations of PNH patients are significantly different, the normal hematopoiesis is exhausted. On account of the defects in normal hematopoietic cells in patients with PNH, the feasibility of using autologous bone marrow transplantation following purging of abnormal clons as a treatment of PNH should be carefully judged, the normal hematopoietic cells alone might not be able to sustain adequate hematopoiesis without further manipulations.
Li
Qiang
Q
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Zhinan
Z
D018952:Antigens, CD34 / Q000032:analysis / Q000276:immunology; D001854:Bone Marrow Cells / Q000473:pathology*; D018957:CD59 Antigens / Q000032:analysis / Q000276:immunology; D002999:Clone Cells; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006457:Hemoglobinuria, Paroxysmal / Q000473:pathology*; D006801:Humans
D018952:Antigens, CD34; D018957:CD59 Antigens