Modulation of neutrophil adhesion to vascular endothelial cells in rat experimental otitis media treated with a macrolide.
OBJECTIVE
We previously reported that erythromycin (EM) affected the adhesion molecules that interact with neutrophils and inhibited neutrophil infiltration in a rat experimental otitis media model.


OBJECTIVE
The purpose of the present study was to examine whether EM affects the adhesion of rat neutrophils to endothelial cells.


METHODS
The adhesion of neutrophils to WK-5 endothelial cells was evaluated. One group of rats was given EM for 2 weeks, while the other group received no treatment. The adhesion of peripheral-blood neutrophils and neutrophils accumulated in the middle ear from these two groups were compared.


RESULTS
Two-week EM pretreatment inhibited adhesion of neutrophils to WK-5 cells. The adhesion of neutrophils accumulated in the middle ear cavity to WK-5 cells was significantly higher than that of peripheral-blood neutrophils from control rats.


CONCLUSIONS
The results suggest that EM affects the adhesion of neutrophils to endothelial cells.
Enomoto
Fuyuki
F
Department of Otorhinolaryngology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyu-ku, Tokyo 113-8421, Japan. fenomoto@med.juntendo.ac.jp
Kin
Ryutaku
R
Kataoka
Takeshi
T
Yamazaki
Yoko
Y
Yokoi
Hindenori
H
Fujimori
Masato
M
Nagaoka
Isao
I
Ichikawa
Ginichirou
G
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology* / Q000627:therapeutic use; D019041:L-Selectin / Q000032:analysis / Q000187:drug effects; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects*; D010033:Otitis Media / Q000188:drug therapy*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000900:Anti-Bacterial Agents; D016177:Macrophage-1 Antigen; D019041:L-Selectin; D004917:Erythromycin