Elastic cartilage engineering using novel scaffold architectures in combination with a biomimetic cell carrier.
Tissue engineering of an elastic cartilage graft that meets the criterion for both structural and functional integration into host tissue, as well as allowing for a clinically tolerable immune response, is a challenging endeavour. Conventional scaffold technologies have limitations in their ability to design and fabricate complex-shaped matrix architectures of structural and mechanical equivalence to elastic cartilage found in the body. We attempted to investigate the potential of conventionally isolated and passaged chondrocytes (2D environment) when seeded and cultured in combination with a biomimetic hydrogel in a mechanically stable and biomimetic composite matrix to form elastic cartilage within ectopic implantation sites. In vitro cultured scaffold/hydrogel/chondrocytes constructs showed islets of cartilage and mineralized tissue formation within the cell-seeded specimens in both pig and rabbit models. Specimens with no cells seeded showed only vascularized fibrous tissue ingrowth. These studies demonstrated the potential of such scaffold/hydrogel/cell constructs to support chondrogenesis in vivo. However, it also showed that even mechanically stable scaffolds do not allow regeneration of a large mass of structural and functional cartilage within a matrix architecture seeded with 2D passaged chondrocytes in combination with a cell biomimetic carrier. Hence, future experiments will be designed to evaluate an initial 3D culture of chondrocytes, effect on cell phenotype and their subsequent culture within biomimetic 3D scaffold/cell constructs.
Hutmacher
Dietmar W
DW
Division of Bioengineering, Faculty of Engineering and Department of Orthopaedics, National University of Singapore, E3-05-15-10-10 Kent Ridge Crescent, Singapore 119260, Singapore. biedwh@nus.edu.sg
Ng
Kee Woei
KW
Kaps
Christian
C
Sittinger
Michael
M
Kläring
Svea
S
D000328:Adult; D000818:Animals; D040761:Biomimetic Materials; D002356:Cartilage / Q000166:cytology / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology / Q000637:transplantation; D004337:Drug Carriers; D004548:Elasticity; D005260:Female; D006801:Humans; D008858:Microscopy, Phase-Contrast; D011817:Rabbits; D012272:Ribs; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous
D004337:Drug Carriers