title: Expression pattern of glypican-1 mRNA after brain injury in mice.
abstract: Glypican-1, a heparan sulfate proteoglycan, is expressed in various tissues including developing and postnatal central nervous system. It serves as a receptor for heparin-binding molecules such as fibroblast growth factors (FGFs). We investigated whether glypican-1 was expressed after brain injury in adult mice. In situ hybridization study showed that glypican-1 mRNA was expressed in the region surrounding necrotic tissue, and that the signal intensity peaked 7 days after the cryo-injury. In addition, both FGF-2 and amyloid precursor protein (APP) were concurrently upregulated and colocalized with glypican-1 mRNA. Since FGF-2 and APP can bind to glypican-1 in vitro, the present study suggested that their autocrine/paracrine interactions with glypican-1 may be involved in neuronal regeneration and/or neurite-outgrowth inhibition after brain injury.
authors.affiliation: Department of Orthopedic Surgery, Fukushima Medical University, School of Medicine, Fukushima 960-1295, Japan. shagino@fmu.ac.jp
authors.forename: Seita
authors.initials: S
authors.lastname: Hagino
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Nobuko
authors.initials: N
authors.lastname: Sakai
authors.forename: Sachihiko
authors.initials: S
authors.lastname: Yokoya
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Hikake
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Akio
authors.initials: A
authors.lastname: Wanaka
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D019898:Autocrine Communication / Q000235:genetics; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D005911:Gliosis / Q000378:metabolism / Q000503:physiopathology; D020439:Growth Cones / Q000378:metabolism / Q000648:ultrastructure; D006133:Growth Substances / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009416:Nerve Regeneration / Q000235:genetics*; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D020127:Recovery of Function / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D006133:Growth Substances; D019812:Heparan Sulfate Proteoglycans; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2