title: Characterization of a small cryptic plasmid, pHP51, from a Korean isolate of strain 51 of Helicobacter pylori.
abstract: The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because it lacked known functional domains or sequence similarity with other known proteins, although it had a HPFXXGNG motif that was also found in the cAMP-induced filamentation (fic) gene. Three kinds of repeats were present on the plasmid outside of the ORFs, including the R1 and R2 repeats that are common in H. pylori plasmids. One 100-bp sequence detected in the noncoding region of pHP51 was highly similar to the genomic sequence of H. pylori 26695.
authors.affiliation: Department of Microbiology, Gyeongsang Institute of Health Science, Gyeongsang National University College of Medicine, 90 Chiram-dong, Jinju, Gyeongsangnam-do 660-751, South Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Sang-Haeng
authors.initials: SH
authors.lastname: Choi
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Byun
authors.forename: Seung-Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Ye-Hyoung
authors.initials: YH
authors.lastname: Park
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Park
authors.forename: Sun-kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Jeong-Uck
authors.initials: JU
authors.lastname: Park
authors.forename: Hyung-Lyun
authors.initials: HL
authors.lastname: Kang
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Gyung-Hyuck
authors.initials: GH
authors.lastname: Ko
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Bae
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004265:DNA Helicases / Q000235:genetics; D004268:DNA-Binding Proteins; D016480:Helicobacter pylori / Q000235:genetics*; D007723:Korea; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases