New antihypertensive peptides isolated from rapeseed.
Four potent angiotensin converting enzyme (ACE) inhibitory peptides, IY, RIY, VW and VWIS, were isolated from subtilisin digest of rapeseed protein. Among them RIY and VWIS are new peptides with IC(50) 28 and 30 microM, respectively. All isolated peptides lowered blood pressure of spontaneously hypertensive rats (SHR) following oral administration. The maximum effect in the case of RIY was observed 4h after administration, while maximum effect of other peptides on blood pressure occurred 2h after administration. Furthermore, the antihypertensive effect of RIY was observed even in old rats, in which ACE inhibitors become less effective, suggesting that a different mechanism other than ACE inhibition is also involved in lowering blood pressure by this peptide. Subtilisin digest of rapeseed protein also significantly lowered blood pressure of SHR after oral administration of a single dosage 0.15 g/kg, exerting maximum antihypertensive effect 4h after administration. This digest appears promising as a functional food, which may be useful in the prevention and treatment of hypertension.
Marczak
Ewa D
ED
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Usui
Hachiro
H
Fujita
Hiroyuki
H
Yang
Yanjun
Y
Yokoo
Megumi
M
Lipkowski
Andrzej W
AW
Yoshikawa
Masaaki
M
D000284:Administration, Oral; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D029741:Brassica rapa / Q000737:chemistry*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D020128:Inhibitory Concentration 50; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D013552:Swine; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010446:Peptide Fragments; D010455:Peptides