title: Genotyping of TF*Dchi allele in human transferrin polymorphism.
abstract: Transferrin (TF) polymorphism, one of the most useful genetic markers, have been studied extensively. TF*Dchi allele is widely distributed both among east Asian populations and American Indian populations. The TFDchi peptide was characterized by replacement of His by Arg at position 300 by amino acid sequencing. In the present study, one base substitution at the 956th nucleotide from the first nucleotide in the starting codon that induced His300Arg exchange was confirmed by direct DNA sequencing. The genotyping method used to detect the TF*Dchi allele involved the use of PCR-RFLP and restriction enzyme Acc II. Analysis of the 1765th nucleotide, which determines the common TF alleles, TF*C1 and TF*C2, in TF*Dchi cDNA indicated that the TF*Dchi allele is derived from the TF*C1 allele.
authors.affiliation: Division of Veterinary Surgery, Nippon Veterinary and Animal Science University, Tokyo 180-8602, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsughida
authors.forename: S
authors.initials: S
authors.lastname: Ikemoto
authors.forename: E
authors.initials: E
authors.lastname: Kajii