Effects of a thermal injury on brain and blood nitric oxide (NO) content in the rat.
In the present study, the effects of a thermal injury on the nitric oxide (NO)-ergic system was investigated in freely moving rats. Using a voltammetric method allowing direct and in situ NO measurements, a significant decrease in cortical NO concentration was observed during the 24h following burning procedure. Since in the burning procedure halothane was employed, it was verified that this anaesthetic did not induce significant effect on cortical NO level. Experiments conducted in ex vivo conditions showed that blood NO and nitrites (NO(2)(-)) + nitrates (NO(3)(-)) concentrations increased strongly after burn injury while hypothalamic inducible NO-synthase (NOS(2)) mRNA level decreased significantly. A thermal injury was thus accompanied by a rapid impairment of the NO-ergic pathways, which might partly have been responsible for numerous changes occurring after burn injury.
Lestaevel
Philippe
P
Département de Radiobiologie et de Radiopathologie, Centre de Recherches du Service de Santé des Armées Emile Pardé, BP 87, 38702 La, Tronche, France. philippe.lestaevel@irsn.fr
Agay
Diane
D
Peinnequin
André
A
Cruz
Catherine
C
Cespuglio
Raymond
R
Clarençon
Didier
D
Multon
Eric
E
Chancerelle
Yves
Y
D000818:Animals; D002056:Burns / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D006221:Halothane / Q000494:pharmacology; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009573:Nitrites / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D009566:Nitrates; D009573:Nitrites; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D006221:Halothane