[A retrospective analysis of population type variability of the region V3 gp120 in HIV infected patients with a unified infection focus].
Regions of HIV-1 genome, encoding the V3 gp120 region, were sequenced in materials that were sampled from persons belonging to the category of individuals infected from a single source during the Rostov-Elista outbreak. The samples were obtained in 1991-1992 (10 pieces) and in 2000-2001 (16 pieces), which amounts to 8% and 13%, respectively, of a total number of patients infected by the beginning of 1990. It was established that the level of the population variability of amino-acid sequences in region V3 amounted, in 1991-1992, to 5.2% and increased to 9.1% in the samples of 2000-2001. A comparison of amino-acid sequences in region V3 from the collections of 1991-1992 and of 2000-2001 revealed the below amino-acid substitutions: from Ser13 to His, from Ley14 to Phe, from Phe to Ley and from Ley to Ala. An analysis of V3 B-epitope showed that the basic trend of its evolution consists in reciprocal transitions of L to F in position 14 and of F to L in position 20. Such substitutions ensure further changes at the "top" of the V3-loop from GPG to APG.
Romashkin
P A
PA
Saukhat
S P
SP
Shemshura
A B
AB
Pazilin
A S
AS
Matiash
E V
EV
Garaev
M M
MM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D015331:Cohort Studies; D004196:Disease Outbreaks; D000939:Epitopes / Q000032:analysis; D014644:Genetic Variation; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000235:genetics* / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology; D012189:Retrospective Studies; D012426:Russia / Q000453:epidemiology; D016415:Sequence Alignment
D000596:Amino Acids; D000939:Epitopes; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments