title: [Inhibition property of a polypeptide APS against phytopathogenic fungi and the possible antifungal mechanism].
abstract: OBJECTIVE
  To investigate the inhibition property of a novel antifungal polypeptide APS in vitro and its possible antifungal mechanism.
  
  
  METHODS
  The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of APS against different fungi were detected by microdilution test. And the voltaic change of planar lipid bilayer was observed during APS treatment.
  
  
  RESULTS
  APS at low MIC and MBC was shown to have effects on phytopathogenic fungi tested except aspergillus fumigatus, the minimum values of MIC and MBC being 1.43 micrograms/ml and 1.78 micrograms/ml respectively. After APS was put in, the transmembrane current could be recorded.
  
  
  CONCLUSIONS
  APS has strong inhibitory effects on the germination of spores of several phytopathogenic fungi. Its antifungal mechanism is likely to form persistent pores on the cell membrane, thus causing membrane permeation and the death of fungi.
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Qiu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
authors.forename: Qiou
authors.initials: Q
authors.lastname: Wei
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D008051:Lipid Bilayers; D008826:Microbial Sensitivity Tests; D010455:Peptides
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D008051:Lipid Bilayers; D010455:Peptides