title: Gcn4 occupancy of open reading frame regions results in the recruitment of chromatin-modifying complexes but not the mediator complex.
abstract: Eukaryotic transcriptional activators usually recognize short DNA motifs, which are not only located within promoter regions, but also scattered throughout the genome. Assuming that the function of activators at non-promoter regions is wasteful and perhaps harmful, one can ask whether such binding is somehow prevented or if transcription is blocked at a downstream step. Here, we show that the yeast transcriptional activator Gcn4 is associated in vivo with several non-promoter euchromatic sites. This association results in the recruitment of the SAGA (Spt3/Ada/Gcn5/acetyltransferase) complex and the consequent activity of the Gcn5 histone acetyltransferase. The functional recruitment of the Swi/Snf nucleosome-remodelling complex was also evident at sites located in positioned nucleosomes. We show that this assemblage of coactivator complexes is not productive because of the absence of core promoter elements, other than the TATA box, that are required for stable mediator recruitment.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, PO Box 1527, Heraklion 711 10, Crete, Greece.
authors.forename: Irini
authors.initials: I
authors.lastname: Topalidou
authors.forename: George
authors.initials: G
authors.lastname: Thireos
mesh_terms: D000251:Adenosine Triphosphatases; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051548:Histone Acetyltransferases; D009687:Nuclear Proteins; D016366:Open Reading Frames / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D000251:Adenosine Triphosphatases; D002843:Chromatin; D004247:DNA; D004268:DNA-Binding Proteins; D051548:Histone Acetyltransferases; D009687:Nuclear Proteins; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C499333:SNF2 protein, S cerevisiae