Nucleotide sequence and phylogenetic classification of candidate human papilloma virus type 92.
From a basal cell carcinoma (BCC) the complete genome of candidate human papillomavirus (HPV) type 92 was characterized. Phylogenetically, the candidate HPV 92 was relatively distantly related to other cutaneous HPV types within the B1 group. By quantitative real time PCR, 94 viral copies were present per cell in the BCC and another BCC contained 1 viral copy per cell. Lower copy numbers were found in two solar keratoses (1 copy per 33 cells and 1 copy per 60 cells) and two squamous cell carcinomas (1 copy per 436 cells and 1 copy per 1143 cells). The high viral load of HPV 92 in two BCCs differs from the low amount of HPV DNA reported from nonmelanoma skin cancers.
Forslund
Ola
O
Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden. ola.forslund@mikrobiol.mas.lu.se
Antonsson
Annika
A
Higgins
Geoff
G
Ly
Hoang
H
Delius
Hajo
H
Hunziker
Andreas
A
de Villiers
Ethel Michele
EM
D002280:Basal Cell Carcinoma / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012867:Skin / Q000821:virology; D012878:Skin Neoplasms / Q000821:virology
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral