Exploring the molecular basis of selectivity in A1 adenosine receptors agonists: a case study.
Adenosine is a naturally occurring purine nucleoside that has a wide variety of well-documented regulatory functions and physiological roles. Selective activation of the adenosine A1 receptor has drawn attention in drug discovery for the therapeutic effects on neural and cardiovascular disorders. We have developed a model of the human A1 adenosine receptor using bovine rhodopsin as a template. A flexible docking approach has been subsequently carried out for evaluating the molecular interactions of twenty-one selective A1 agonists with the receptor model. The results of these studies are consistent with mutational and biochemical data. In particular, they highlight a wide hydrogen-bonding network between the nucleoside portion of the ligands and the A1 receptor as well as key amino acids for hydrophobic interactions with the different N6-groups of the agonists. The models presented here provide a detailed molecular map for the selective stimulation of the adenosine A1 receptor subtype and a steady basis for the rational design of new A1 selective ligands.
Giordanetto
Fabrizio
F
Centre for Computational Science, Department of Chemistry, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom.
Fossa
Paola
P
Menozzi
Giulia
G
Schenone
Silvia
S
Bondavalli
Francesco
F
Ranise
Angelo
A
Mosti
Luisa
L
D000241:Adenosine / Q000737:chemistry; D058907:Adenosine A1 Receptor Agonists; D000595:Amino Acid Sequence; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D043682:Receptor, Adenosine A1 / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D058907:Adenosine A1 Receptor Agonists; D043682:Receptor, Adenosine A1; D000241:Adenosine