title: Characteristics of humic substances in the Kuji River waters as determined by high-performance size exclusion chromatography with fluorescence detection.
abstract: Direct measurement by high-performance size exclusion chromatography with fluorescence detection was applied to the characterization of humic substances in river waters from the Kuji River system, which runs through forest hills and an agricultural plain in Japan. The monitoring wavelength of excitation 320 nm and emission 430 nm corresponds to the fluorescence maxima for aquatic fulvic acid. Chromatograms of the river waters showed four peaks; each peak position was in good agreement among these samples. Peak height ratios for the samples from the upstream Kuji River and its tributaries were different from those of the midstream and downstream sections of the Kuji River, which may reflect differences in the characteristics of humic substances and other organic materials supplied from soil to river.
authors.affiliation: Department of Environmental Sciences, Japan Atomic Energy Research Institute, 2-4 Shirakata-Shirane, Tokai-mura, Naka-gun, 319-1195 Ibaraki, Japan. nagao@ees.hokudai.ac.jp
authors.forename: Seiya
authors.initials: S
authors.lastname: Nagao
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Ueno
authors.forename: Hikaru
authors.initials: H
authors.lastname: Amano
mesh_terms: D002850:Chromatography, Gel; D005453:Fluorescence; D006812:Humic Substances / Q000032:analysis*; D007564:Japan; D045483:Rivers; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D006812:Humic Substances; D014873:Water Pollutants