Altered patterns of T cell cytokine production induced by relapsed pre-B ALL cells.
Relapse of pediatric acute lymphoblastic leukemia (ALL) remains a significant clinical problem. The graft-versus-leukemia (GVL) effect after hematopoietic stem cell transplantation indicates that immune mechanisms may play a role in the control of ALL blasts. In this study, we analyzed primary diagnostic and relapsed pre-B ALL samples for the surface expression of several molecules implicated in immune responses and for the induction of allogeneic T cell responses. There were no significant differences in the expression of CD11a, CD40, CD80 and CD86 or MHC classes I and II molecules between the diagnostic and relapsed samples. We found no significant differences in the overall ability of diagnostic and relapsed pre-B ALL samples to induce T cell proliferation and cytokine production. However, in the case of T cell responses induced by diagnostic ALL samples, there was excellent correlation between proliferation and production of all cytokines analyzed. In the case of relapsed samples, the only correlation obtained was with IL-5. This observation indicates that the nature of the immune response generated by relapsed ALL cells in an allogeneic setting differs from that obtained with diagnostic samples, suggests a biasing towards a Th2 response may contribute to the evasion of effective immune responses by relapsed ALL.
Reid
Gregor S D
GS
Department of Pediatrics, University of British Columbia and B.C.'s Children's Hospital, 4480 Oak Street, BC V5Z 4H4 Vancouver, Canada.
Terrett
Luke
L
Alessandri
Angela J
AJ
Grubb
Stacey
S
Stork
Linda
L
Seibel
Nita
N
Gaynon
Paul
P
Schultz
Kirk R
KR
D016207:Cytokines / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D005805:Genes, MHC Class I; D006684:HLA-DR Antigens / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D009364:Neoplasm Recurrence, Local / Q000276:immunology* / Q000378:metabolism; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D016207:Cytokines; D017931:DNA Primers; D006684:HLA-DR Antigens