title: CD8 T cell-specific downregulation of histone hyperacetylation and gene activation of the IL-4 gene locus by ROG, repressor of GATA.
abstract: Chromatin remodeling of type 2 cytokine gene loci occurs during differentiation of naive CD4 and CD8 T cells into type 2 helper (Th2) and cytotoxic (Tc2) T cells. IL-4 production and histone hyperacetylation in IL-4-associated nucleosomes in developing Tc2 cells were significantly lower than those of Th2 cells; however, cytokine production and histone hyperacetylation of IL-5 and IL-13 genes were equivalent. Developing Tc2 cells expressed lower GATA3 levels and dramatically increased levels of repressor of GATA (ROG). A ROG response element in the IL-13 gene exon 4 displayed Tc2-specific binding of ROG, HDAC1, and HDAC2 and exhibited repression of IL-4 gene activation. Thus, ROG may confer CD8 T cell-specific repression of histone hyperacetylation and activation of the IL-4 gene locus.
authors.affiliation: Department of Molecular Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Omori
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Yamashita
authors.forename: Masamichi
authors.initials: M
authors.lastname: Inami
authors.forename: Maki
authors.initials: M
authors.lastname: Ukai-Tadenuma
authors.forename: Motoko
authors.initials: M
authors.lastname: Kimura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nigo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hosokawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D050990:GATA3 Transcription Factor; D005786:Gene Expression Regulation; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D018793:Interleukin-13 / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics*; D020935:MAP Kinase Signaling System; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D006657:Histones; D018793:Interleukin-13; D012097:Repressor Proteins; C406863:Rog protein, mouse; D015534:Trans-Activators; D015847:Interleukin-4; D004247:DNA; D019908:Proto-Oncogene Proteins c-raf; D019703:Calcineurin