Construction of targeting vector and expression of green fluorescent protein in the silkworm, Antheraea pernyi.
The transcription initiation site of Anrheraea pernyi was identified, and a gene targeting vector was constructed. The green fluorescent protein (GFP) gene was inserted into this vector under the control of a fibroin promoter. This recombinant vector was used to target the GFP gene to the fibroin region of the silkworm genome. The DNA was injected into the testes during the pupae, and was also transferred into the egg via the sperm during fertilization. The analysis showed that the GFP gene was integrated into the fibroin gene on the genome by homologous recombination, and was expressed in the silk gland.
Li
Wenli
W
Bioengineering Department, Dalian University of Technology, Dalian 116012, Liaoning Province, PR China. liwenli2002@yahoo.com
Jin
Liji
L
An
Lijia
L
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005349:Fibroins / Q000235:genetics; D015870:Gene Expression; D018390:Gene Targeting; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D014158:Transcription, Genetic
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D004247:DNA; D005349:Fibroins