[Comparative genome analysis of Helicobacter pylori strains].
DNA macroarrays were used to characterize 17 Helicobacter pylori strains isolated in four geographic regions of Russia (Moscow, St. Petersburg, Kazan, and Novosibirsk). Of all genes, 1272 (81%) proved to occur in all strains and to constitute a functional core of the genome, and 293 (18.7%) were strain-specific and greatly varied among the H. pylori strains. Most (71%) of the latter had unknown functions; the remainder included restriction-modification genes (3-9%), transposition genes (2-4%), and genes coding for outer membrane proteins (2-4%). The Russian H. pylori strains did not differ in genome organization or in the number and distribution of strain-specific genes from strains isolated in other countries.
Momynaliev
K T
KT
Institute of Physico-Chemical Medicine, Ministry of Health of the Russian Federation, Moscow, 119992 Russia. dhoroshun@nm.ru
Smirnova
O V
OV
Kudriavtseva
L V
LV
Govorun
V M
VM
D001426:Bacterial Proteins / Q000235:genetics; D023061:Gene Order; D005798:Genes, Bacterial; D014644:Genetic Variation; D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D008562:Membrane Glycoproteins / Q000235:genetics; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012426:Russia; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C077520:VapD protein, Bacteria