[Using two-dimensional gel electrophoresis to analyze the regeneration microenvironment of facial nerve and its response to acupuncture].
OBJECTIVE
To investigate the distribution of proteins in the regeneration microenvironment of facial nerve and its response to acupuncture.


METHODS
We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two-dimensional gel electrophoresis.


RESULTS
We found that most proteins in the regeneration fluid were under relative molecular mass 80 x 10(3) and in the range of pI 4-7. An average of 800 +/- 53 protein spots was found in the control group compared to the 850 +/- 78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups.


CONCLUSIONS
There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve.
Yang
Hui
H
Otonasolaryngeal Department, West China Hospital, Sichuan Universty, Chengdu 610041, China.
Yang
Cairong
C
Lu
Xiaofeng
X
Liang
Chuanyu
C
Gao
Bingqing
B
D026881:Acupuncture; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005154:Facial Nerve / Q000502:physiology / Q000503:physiopathology*; D020220:Facial Nerve Injuries / Q000503:physiopathology; D005260:Female; D008297:Male; D009416:Nerve Regeneration; D011506:Proteins / Q000032:analysis*; D011817:Rabbits
D011506:Proteins