title: Caries progression and inhibition in human and bovine root dentine in situ.
abstract: Since the use of bovine instead of human dentine to evaluate cariogenic and anticariogenic substances is not well established, this in situ study was conducted. Eleven volunteers wore palatal acrylic devices containing 4 dentine slabs (2 human and 2 bovine). Sucrose solution (20%) was dripped over all slabs 4 times a day, simulating a cariogenic challenge. Dentifrice slurries, fluoridated or not, were dripped over specified dentine slabs 3 times a day to evaluate caries reduction. After 14 days, the biofilm formed on the dentine slabs was collected for microbiological analysis. In dentine, mineral loss (DeltaZ) and lesion depth (LD) were determined by cross-sectional microhardness and by polarized light microscopy, respectively. The total streptococci and mutans streptococci counts in the biofilm formed either on human or on bovine slabs, whether treated or not with fluoride dentifrice, were not statistically different. The DeltaZ and the LD values of dentine treated with fluoride dentifrice were significantly lower than the values of dentine treated with non-fluoride dentifrice. The differences in the DeltaZ and LD values between the human and bovine dentine were not statistically significant. The results suggest that bovine dentine can be used instead of human to evaluate caries development and inhibition.
authors.affiliation: Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
authors.forename: A T
authors.initials: AT
authors.lastname: Hara
authors.forename: C S
authors.initials: CS
authors.lastname: Queiroz
authors.forename: A F
authors.initials: AF
authors.lastname: Paes Leme
authors.forename: M C
authors.initials: MC
authors.lastname: Serra
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000818:Animals; D018441:Biofilms; D002326:Cariogenic Agents / Q000009:adverse effects; D002327:Cariostatic Agents / Q000627:therapeutic use; D002417:Cattle; D015169:Colony Count, Microbial; D003802:Dentifrices / Q000627:therapeutic use; D003804:Dentin / Q000382:microbiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D005459:Fluorides / Q000627:therapeutic use; D006244:Hardness; D006801:Humans; D008859:Microscopy, Polarization; D017213:Root Caries / Q000382:microbiology / Q000503:physiopathology* / Q000517:prevention & control; D018709:Statistics, Nonparametric; D013291:Streptococcus / Q000254:growth & development; D013295:Streptococcus mutans / Q000254:growth & development; D013395:Sucrose / Q000009:adverse effects
chemical_list: D002326:Cariogenic Agents; D002327:Cariostatic Agents; D003802:Dentifrices; D013395:Sucrose; D005459:Fluorides