title: [Potent and specific inhibition of hepatitis B virus antigen expression by RNA interference].
abstract: OBJECTIVE
  To develop an RNAi approach that specifically targets the core gene sequence of hepatitis B virus by synthesizing short interfering RNA (siRNA) in vivo, and to assess the inhibitory effect of this siRNA on HBV replication.
  
  
  METHODS
  The eukaryotic expression plasmid pHBV1.3, which contains 1.3-fold-overlength genome of HBV, were cotransfected into HepG2 cells with either the RNAi plasmid pSIHBV/C or unrelated control plasmid pSI. At 24, 48, 72 hours post transfection (p.t.), the levels of HBsAg and HBeAg in the cell culture medium were determined with Abbott MEIA Kits. The expression of intracellular viral proteins was also determined by immunofluorescence staining.
  
  
  RESULTS
  Successfully constructed expressing siRNA vector pSIHBV which targeted the core gene of Hepatitis B virus.The introduction of RNAi plasmid was shown to efficiently and specifically inhibit the synthesis of surface and e antigen of HBV by Abbot MEIA kits, with inhibitory rates at 92%, 85% peaking at 24 hours p.t. Immunofluorescence staining showed that intracellular synthesis of viral antigen was sharply reduced to nearly background levels when the ratio of pSIHBV/C and pHBV1.3 was at 1:20, whereas the control vector did not exhibit any inhibitory effect on the replication and expression of HBV.
  
  
  CONCLUSIONS
  Our results demonstrate that the short interfering RNA targeting HBV core gene exerts robust inhibition on HBV viral replication, suggesting that RNAi-based anti-HBV replication strategy may represent a potentially efficacious approach to the clinical management of HBV infection.
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Tang
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
authors.forename: Bing-qiang
authors.initials: BQ
authors.lastname: Zhang
authors.forename: Ge
authors.initials: G
authors.lastname: Yan
authors.forename: Tong-Chuan
authors.initials: TC
authors.lastname: He
mesh_terms: D005455:Fluorescent Antibody Technique; D015316:Genetic Therapy; D006509:Hepatitis B / Q000628:therapy*; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000502:physiology; D006801:Humans; D034622:RNA Interference; D014779:Virus Replication
chemical_list: D006512:Hepatitis B Core Antigens