Matrix metalloproteinase profile in patients with Creuztfeldt-Jakob disease.
Preliminary findings suggest that abnormalities in matrix metalloproteinase (MMP) activity may be found in the cerebrospinal fluid (CSF) of patients with Creutzfeldt-Jakob disease (CJD). In this study of 16 subjects with CJD and 16 age-, and sex-matched controls, we determined the presence of MMP-2 and MMP-9 in their active and proenzyme forms, the relative levels of MMP-3 and four inhibitors of MMP activity (TIMP-1, TIMP-2, TIMP-3 and TIMP-4), and the concentration of 4-3-3 protein. The methodology used involved zymography and immunological techniques. The results indicate that, compared with controls, CJD patients have a significantly higher positive frequency of pro-MMP-9 and of the active form of MMP-2, along with significantly higher levels of TIMP-1 and TIMP-2, classical inhibitors of MMP-9 and MMP-2, respectively. We also found a positive correlation between 14-3-3 protein concentration and that of TIMP-1 and TIMP-2 levels (correlation coefficients of 0.793 and 0.798, respectively). These results suggest that abnormalities in MMP and TIMP profiles may be helpful in the biochemical characterisation of CJD.
Kettlun
A
A
Department of Biochemistry and Molecular Biology, School of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile.
Collados
L
L
García
L
L
Cartier
L A
LA
Wolf
M E
ME
Mosnaim
A D
AD
Valenzuela
M A
MA
D048948:14-3-3 Proteins; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000134:cerebrospinal fluid*; D008875:Middle Aged; D019714:Tissue Inhibitor of Metalloproteinases / Q000134:cerebrospinal fluid*; D014446:Tyrosine 3-Monooxygenase / Q000134:cerebrospinal fluid*
D048948:14-3-3 Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D014446:Tyrosine 3-Monooxygenase; D020782:Matrix Metalloproteinases