Sesquiterpene lactone from Wunderlichia crulsiana inhibits the respiratory burst of leukocytes triggered by distinct biochemical pathways.
The sesquiterpene lactone tubiferin was chemically purified from the brazilian native plant Wunderlichia crulsiana and identified by NMR and GC/MS data. Its ability to inhibit the respiratory burst of peritoneal inflammatory polymorphonuclear leukocytes (PMN) stimulated upon addition of phorbol miristate acetate (PMA), opsonized zymosan (OZ), and N-formyl-methionyl-leucyl-phenylalanine (fMLP) was evaluated. The tubiferin inhibition was more pronounced when PMN were stimulated through the protein kinase C pathway (PMA) compared to the alternative complement pathway (OZ). The inhibition when PMN were triggered by a chemoattractant stimulus (fMLP) was similar to that achieved with OZ-stimulated phagocytes. Tubiferin showed dose-dependent effects on the PMN respiratory burst triggered by the three different substances, and also decreased substantially the carrageenan-induced mice paw edema.
Nuñez
Cecilia Veronica
CV
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Zacheu
Fabíola Maria
FM
Pinto
Ernani
E
Roque
Nídia Franca
NF
Colepicolo
Pio
P
Brigagão
Maísa Ribeiro Pereira Lima
MR
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry; D001938:Brazil; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D006614:Hindlimb; D007213:Indomethacin / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D026082:Singlet Oxygen / Q000032:analysis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; C477275:tubiferin; D026082:Singlet Oxygen; D007213:Indomethacin