Expression of interleukin-10 and interleukin-12 in piglets experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV).
The expression of mRNA encoding interleukin-10 (IL-10) and IL-12 was studied, by the reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled cDNA probe, in formalin-fixed, paraffin wax-embedded lung tissue from piglets inoculated intranasally with a Korean isolate (North American genotype) of porcine reproductive and respiratory syndrome virus (PRRSV). IL-12p35-positive cells were detected in the lung at 1 day post-inoculation (dpi), their number increasing at 5 dpi, and rapidly decreasing thereafter. In contrast, IL-10- and IL-12p40-positive cells were detected in the lung at 1 dpi, their number increasing at 3 dpi, and rapidly decreasing thereafter. Hybridization signals for IL-10, IL-12p35 and IL-12p40 were always associated with inflammation. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected piglets and in normal lung from control piglets. In-situ hybridization in serial sections of lung tissues indicated close co-localization of PRRSV and these cytokines in interstitial pneumonia. The results suggest that the expression of IL-10 and IL-12 plays a role in pulmonary defence mechanisms against PRRSV infection.
Chung
H-K
HK
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul, South Korea.
Chae
C
C
D000818:Animals; D002452:Cell Count; D015967:Gene Expression Regulation, Viral; D017403:In Situ Hybridization; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D017563:Lung Diseases, Interstitial / Q000378:metabolism / Q000473:pathology / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000473:pathology / Q000635:transmission; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D012333:RNA, Messenger; D012367:RNA, Viral; D016753:Interleukin-10; D018664:Interleukin-12