Rapid and sensitive determination of phenol in honey by high-performance liquid chromatography with fluorescence detection.
The objective of this research was to develop a novel high-performance liquid chromatographic (HPLC) method involving a simple sample preparation procedure for the rapid, low-cost, and sensitive quantitation of phenol in honey at levels of regulatory and practical importance. After proper dilution of honey with water, the samples were analyzed by a gradient HPLC system, using a reversed-phase column with fluorescence detection at excitation and emission wavelengths of 270 and 300 nm, respectively. The eluents applied were water-acetonitrile-85% orthophosphoric acid (10:10:0.01, v/v/v) and water-85% orthophosphoric acid (20:0.01, v/v). The retention time of phenol was found to be 14.1 min, and the limit of quantitation for phenol in honey was set at 5 microg/kg. Overall recovery was 98%. The proposed method has been successfully applied to real sample analysis.
Gyorik
Márta
M
Institute of Food Science, Faculty of Agricultural and Food Sciences, University of West Hungary, 15-17 Lucsony Street, 9200 Mosonmagyaróvár, Hungary. gyorik@mtk.nyme.hu
Herpai
Zoltán
Z
Szécsényi
Ildikó
I
Varga
László
L
Szigeti
Jeno
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006722:Honey / Q000032:analysis*; D007202:Indicators and Reagents; D019800:Phenol / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D019800:Phenol