title: [Ziehl-Neelsen-Victoria blue-iodine green compound staining method showing leprotic bacillus].
abstract: OBJECTIVE
  To compare compound staining methods showing leprotic bacillus, elastic fibre and cell nucleus etc. in skin biopsy tissue.
  
  
  METHODS
  A compound staining dye composed of Ziehl-Neelsen Phenol Fuchsin, Victoria blue, iodine green and Martius yellow were used in paraffin-embedding skin biopsy tissue.
  
  
  RESULTS
  Leprotic bacilli showed red; cell nuclei, light green; cell cytoplasm, colorless; elastic fibres around tuberculoid nodules, blue; and essential substances, light yellow in skin biopsy tissue.
  
  
  CONCLUSIONS
  Because the color was single in Ziehl-Neelsen staining method, its contrast was poor. The ZN-VB-IG compound staining method may show leprotic bacilli, elastic fibres and cell nuclei etc. distinctly. Therefore, this is a reliable and nice color contrast compound staining method.
authors.affiliation: Department of Pathology, First Affiliated Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Gong
authors.forename: Minghua
authors.initials: M
authors.lastname: Zhu
authors.forename: Jianming
authors.initials: J
authors.lastname: Zheng
authors.forename: Rongzhou
authors.initials: R
authors.lastname: Zhan
authors.forename: Wanhe
authors.initials: W
authors.lastname: Lin
mesh_terms: D006801:Humans; D009166:Mycobacterium leprae / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*