Enhancement of the sliding velocity of actin filaments in the presence of ATP analogue: AMP-PNP.
The sliding velocity of actin filaments was found to increase in the presence of ATP analogues. At 0.5 mM ATP, the presence of 2.0 mM of AMP-PNP enhanced the filament velocity from 3.2 up to 4.5 microm/s. However, 2 mM ADP decreased the velocity down to 1.1 microm/s. The results suggest that the complex conformations of myosin cross-bridges interacting with an actin filament in the presence of ATP analogues makes the entire filament move faster.
Sakamaki
Jun'ichi
J
Department of BioEngineering, Nagaoka University of Technology, Nagaoka 940-2188, Japan.
Honda
Hajime
H
Imai
Ei'ichi
E
Hatori
Kuniyuki
K
Shimada
Katsuhiko
K
Matsuno
Koichiro
K
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000502:physiology*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000737:chemistry; D009218:Myosins / Q000378:metabolism / Q000502:physiology; D011817:Rabbits; D016008:Statistical Distributions
D000266:Adenylyl Imidodiphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009218:Myosins