Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA analysis.
Isoenzymes and RAPD (random amplified polymorphic DNA) analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be related to the clonal reproduction of this protozoan.
Rocha
Míriam O
MO
School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. morocha@farmacia.ufmg.br
Gomes
Maria A
MA
Costa
Adriana O
AO
Furst
Cinthia
C
Silva
Edward F
EF
D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis*; D004592:Electrophoresis, Starch Gel; D019353:Endemic Diseases; D005260:Female; D016829:Giardia lamblia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005873:Giardiasis / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan; D007527:Isoenzymes