title: Stage-specific gene expression during Trypanosoma cruzi metacyclogenesis.
abstract: The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression in trypanosomatids. It may also improve the understanding of the mechanisms responsible for the pathology of Chagas disease, and could lead to the identification of new targets for chemotherapy of this disease. We have demonstrated that during metacyclogenesis the expression of several genes is controlled at the translational level by an alternative regulatory mechanism. This mechanism may involve the mobilization of mRNA to the translation machinery. We have been using self-made T. cruzi microarrays to investigate the role of polysomal mobilization in modulating gene expression during metacyclogenesis.
authors.affiliation: Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader, 3775, 81350-010, Curitiba, PR, Brasil.
authors.forename: Andréa Rodrigues
authors.initials: AR
authors.lastname: Avila
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.forename: Sueli Fumie
authors.initials: SF
authors.lastname: Yamada-Ogatta
authors.forename: Viviane
authors.initials: V
authors.lastname: Monteiro-Góes
authors.forename: Stênio Perdigão
authors.initials: SP
authors.lastname: Fragoso
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Krieger
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D008018:Life Cycle Stages / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity