Mast cells in tissue response to dentistry materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement.
Synthetic materials used in dentistry may trigger various inflammatory responses. In order to evaluate biocompatibility, standardized implants of Calcium Hydroxide (CH), Glass Ionomer Cement (GIC) and Light-activated Dental Adhesive (LDA) were surgically introduced into Wistar rats' back bone. Six (experimental) animal groups, five each, and two Sham (S) groups were studied after 15 and 30 days from surgery. In each animal, the density of mast cells and interstitial fibrosis volume was evaluated by quantitative light microscopy. In addition, the interaction between the disk material and its fibrous capsule was evaluated by scanning electron microscopy. The density of mast cells per area (N(A)[mast cells]) was lower in CH group than in LDA group. GIC group displayed N(A)[mast cells] results intermediate between CH and LDA groups (p<0.05). The smallest interstitial fibrosis volume density (Vv[f]) was observed in CH group, then in GIC group, while the greatest in LDA group. After 30 days, the fibrosis in LDA group was 30% higher than in CH group (p<0.05). In S group, discreet fibrosis restricted to surgical area was present, with few mast cells near the vessels. Significant interaction between fibrous capsule and the surrounding disk material was most evident in CH group. The implanted materials induced mast cell migration, distinct fibrosis development, suggesting that CH is the most biocompatible material among those tested.
Mussel
R L De Oliveira
RL
Laboratory of Morphometry & Cardiovascular Morphology, State University of Rio de Janeiro, Av. 28 de Setembro, 87 (fds) - 20551-030 - Rio de Janeiro, RJ, Brasil.
De Sá Silva
E
E
Costa
Andréa Monte Alto
AM
Mandarim-De-Lacerda
C A
CA
D000818:Animals; D001672:Biocompatible Materials; D002126:Calcium Hydroxide / Q000378:metabolism*; D003188:Composite Resins / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005899:Glass Ionomer Cements / Q000378:metabolism*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D008422:Materials Testing; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D019279:Resin Cements / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000378:metabolism / Q000648:ultrastructure
D001672:Biocompatible Materials; D003188:Composite Resins; D005899:Glass Ionomer Cements; D019279:Resin Cements; D002126:Calcium Hydroxide