title: Development of a device for selective removal of CD4+ T cells.
abstract: To control antigen (Ag)-specific immune cells is important in the treatment of autoimmune diseases. In particular, controlling the immune response of autoimmune T cells is effective in the treatment of these diseases. The development of a device that can remove CD4+ T cells specifically by extracorporeal circulation is now in progress, with the aim to deplete autoimmune T cells. We developed a removal material made of polypropylene non-woven fabrics with anti human CD4 monoclonal antibody immobilized on the surface. Using a column packed with the removal material, we succeeded in removing CD4+ T cells specifically from peripheral whole blood by direct perfusion. Moreover, CD4+ T cells can be specifically removed even from blood with lower surface antigen density by in vitro activation.
authors.affiliation: Research and Development Laboratory 2, Asahi Medical Co., Oita, Japan. onodera.hc@om.asahi-kasei.co.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Onodera
authors.forename: Kasumi
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsuo
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Shibuya
mesh_terms: D001327:Autoimmune Diseases / Q000628:therapy; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002478:Cells, Cultured; D004867:Equipment Design; D004869:Equipment Safety; D006801:Humans; D012680:Sensitivity and Specificity