title: Epidemiological survey of Anaplasma platys and Ehrlichia canis using ticks collected from dogs in Japan.
abstract: Detection of Anaplasma platys and Ehrlichia canis in ticks recovered from dogs in Japan was attempted using a species-specific nested PCR based on the 16S rRNA gene. A total of 1211 ticks recovered from 1211 dogs from all over Japan were examined for A. platys and E. canis. Four tick samples from Fukushima, Miyazaki and Kagoshima Prefectures recovered from four different dogs showed a positive reaction for A. platys. Although the four dogs did not show any clinical signs and no blood examination data were available, it is possible that A. platys has already been spread widely in Japan. No positive reactions were observed in any ticks examined for E. canis.
authors.affiliation: Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. inokuma@yamaguchi-u.ac.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Takeshi
authors.initials: T
authors.lastname: Beppu
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Shimada
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Sakata
mesh_terms: D000709:Anaplasma / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000453:epidemiology* / Q000382:microbiology; D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D041061:Ehrlichia canis / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S