[HLA-A genotyping by oligonucleotide arrays].
OBJECTIVE
To investigate HLA genotyping by oligonucleotide arrays.


METHODS
Unsymmetrical PCR was used to amplify HLA-A gene exon 2, 3. The PCR products were used as templates for hybridization. The oligonucleotide probes were spotted on glass to make microarrays. High signal and specific probes were selected. The effects of the length and location of probes on hybridization signal were studied. A set of computer software was designed for scanning and genotype differentiation.


RESULTS
The genotypes of 30 samples analyzed by microarray showed concordance to that by SBT and PCR-SSP.


CONCLUSIONS
HLA-A genotyping by oligonucleotide array is a good method with advantage of high speed, low cost and high flux.
Hu
Shou-wang
SW
Shenzhen Yishengtang Biological Products Co, LTD, Shenzhen 518026, China.
Zhang
Fan
F
Wang
Hui
H
Geng
Yong-yao
YY
Wang
Sheng-qi
SQ
D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
D015234:HLA-A Antigens; D015345:Oligonucleotide Probes