[Influence of the activator of transcription GAL4 on growth and development of embryos and embryonic cells in primary cultures of sand dollar].
In order to solve many tasks of biotechnology, constant lines of the cells of marine invertebrates with a high growth potential are required, which are absent at present. We used the universal activator of transcription gal4 to change the degree of expression of genes of growth factors in embryonic sea urchin cells and, thereby, increase their proliferative activity. The fertilized sea urchin eggs and dissociated embryonic cells at the blastula stage were treated with plasmids containing both the functional gene gal4 and the gene devoid of the regions encoding the activator domain. The transfection of embryonic sea urchin eggs with the functional gene led to cell dedifferentiation and formation of tumor-like structures in the embryos or increased number of embryonic cells in culture. In the cells obtained from the transfected embryos, the pigments were found within two months of cultivation, whose absorption spectrum coincided with that of echinochrome.
Odintsova
N A
NA
Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, ul. Pal'chevskoro 17, Vladivostok, 690041 Russia. anodin@mail.ru
Kiselev
K V
KV
Bulgakov
V P
VP
Kol'tsova
E A
EA
Iakovlev
K V
KV
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004625:Embryo, Nonmammalian / Q000166:cytology; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors