Allele-specific expression of the IL-1 alpha gene in human CD4+ T cell clones.
A number of reports have described the monoallelic expression of murine cytokine genes. Here we describe the monoallelic expression of the human IL-1alpha gene in CD4+ T cells. Analysis of peripheral blood T cell clones derived from healthy individuals revealed that the IL-1alpha gene shows predominantly monoallelic expression. Monoallelic expression was observed in Th0, Th1, and Th2 cell clones. In addition, we demonstrate monoallelic expression in T cell clones from rheumatoid arthritis patients derived from synovial fluid of the knee joint, suggesting that the occurrence of this phenomenon is not different from that in clones derived from healthy individuals. The finding of monoallelic expression of a cytokine gene in human CD4+ T cell clones provides evidence for allele-specific silencing/activation as another layer of regulation of IL-1alpha gene expression.
Bayley
Jean-Pierre
JP
Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
van Rietschoten
Johanna G I
JG
Bakker
Aleida M
AM
van Baarsen
Lisa
L
Kaijzel
Eric L
EL
Wierenga
Eddy A
EA
van der Pouw Kraan
Tineke C T M
TC
Huizinga
Tom W J
TW
Verweij
Cornelis L
CL
D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002999:Clone Cells; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology*; D005838:Genotype; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
D007375:Interleukin-1; C069758:endodeoxyribonuclease Fnu4HI; D015252:Deoxyribonucleases, Type II Site-Specific; C024412:GGTGA-specific type II deoxyribonucleases