title: Structure and oligomeric state of the mammalian tumour-associated antigen UK114.
abstract: The tumour-associated antigen UK114, isolated from goat liver, belongs to the YER057c/YIL051c/YjgF protein family, which has members in both the prokaryotes and eukaryotes. The crystal structure of a mammalian representative, goat UK114, was determined, revealing a trimeric arrangement in the crystal. It was confirmed by ultracentrifugation that UK114 is a trimer in solution. These results are in agreement with the published structures of homologues from unicellular organisms, but contrast with those reported for the rat homologue of UK114, for which a dimeric quaternary structure was proposed.
authors.affiliation: Institute of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
authors.forename: Daniela
authors.initials: D
authors.lastname: Deriu
authors.forename: Christophe
authors.initials: C
authors.lastname: Briand
authors.forename: Edita
authors.initials: E
authors.lastname: Mistiniene
authors.forename: Vytautas
authors.initials: V
authors.lastname: Naktinis
authors.forename: Markus G
authors.initials: MG
authors.lastname: Grütter
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization; D018360:Crystallography, X-Ray; D006041:Goats; D008099:Liver / Q000737:chemistry; D008958:Models, Molecular; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D012996:Solutions; D014461:Ultracentrifugation
chemical_list: D009363:Neoplasm Proteins; D012996:Solutions; C101630:UK114 antigen