Effects of hydrostatic pressure on cultured bovine trabecular meshwork cells.
In order to explore the effects of pressure on trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and subjected to different levels of hydrostatic pressure. The cellular morphology, ultrastructure and phagocytosis were studied with inverted phase-contrast microscopy, light microscopy and transmission electron microscopy, etc. It was found that the cells subjected to 2.0 kPa or 2.67 kPa for 48 h had no remarkable difference as compared with the controls in terms of parameters observed. Those under 4.0 kPa for 24 h showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer. From the above we conclude that trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level.
Jiang
F
F
Department of Ophthalmology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
Wei
H
H
Lu
Y
Y
Zhou
Y
Y
Zhang
Y
Y
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006874:Hydrostatic Pressure; D008853:Microscopy; D010587:Phagocytosis; D014129:Trabecular Meshwork