title: The varicella-zoster virus induces apoptosis in vitro in subpopulations of primary human peripheral blood mononuclear cells.
abstract: Varicella-zoster virus (VZV), a member of Herpesviridae, subfamily alpha-Herpesvirinae, is pathogenic exclusively in the human. Chickenpox is the result of primary infection of VZV. During the viremic stage, VZV infects peripheral blood mononuclear cells (PBMC) and spreads to the periphery. In skin cells it causes typical lesions. Apoptosis has been demonstrated in different cell types by other alpha-herpesviruses. VZV-infected T lymphocytes, B lymphocytes, and monocytes, respectively, were examined in this in vitro study by flow cytometry, immunofluorescence and electron microscopy. All infected cell types showed signs of apoptosis: a lower DNA content, DNA fragmentation, loss of membrane integrity, and an altered nuclear morphology. The results observed led to the suggestion that VZV can induce apoptosis during infection in vivo in the PBMC subpopulations.
authors.affiliation: Institute for Microbiology and Virology, University of Witten/Herdecke, Stockumer Str. 10, 58448, Witten, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: König
authors.forename: Claudia
authors.initials: C
authors.lastname: Hömme
authors.forename: Bärbel
authors.initials: B
authors.lastname: Hauröder
authors.forename: Alexandra
authors.initials: A
authors.lastname: Dietrich
authors.forename: Manfred H
authors.initials: MH
authors.lastname: Wolff
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001562:Benzimidazoles / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000648:ultrastructure; D014645:Herpesvirus 3, Human / Q000472:pathogenicity*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D008854:Microscopy, Electron; D011419:Propidium / Q000378:metabolism
chemical_list: D017304:Annexin A5; D001562:Benzimidazoles; D011419:Propidium; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride