title: Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish.
abstract: We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage lambda causes transposition primarily into the vicinity of the lambda operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.
authors.affiliation: Environmental Biosafety Research Institute, Agricultural Biotechnology Center, Szent-Györgyi Albert St. 4, H-2101 Gödöllo, Hungary.
authors.forename: Mónika
authors.initials: M
authors.lastname: Szabó
authors.forename: Ferenc
authors.initials: F
authors.lastname: Müller
authors.forename: János
authors.initials: J
authors.lastname: Kiss
authors.forename: Carolin
authors.initials: C
authors.lastname: Balduf
authors.forename: Uwe
authors.initials: U
authors.lastname: Strähle
authors.forename: Ferenc
authors.initials: F
authors.lastname: Olasz
mesh_terms: D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D004625:Embryo, Nonmammalian; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D006367:HeLa Cells; D006801:Humans; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D011387:Prokaryotic Cells / Q000502:physiology; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019895:Transposases / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D000071676:Zinc Finger Protein GLI1
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D015513:Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D000071676:Zinc Finger Protein GLI1; C016649:phage repressor proteins; D004247:DNA; D019895:Transposases