title: Association between diabetes type 1 and DQB1 alleles in a case-control study conducted in Montevideo, Uruguay.
abstract: We studied HLA DQB1 allele frequencies and the relative risk (RR) of various genotypes in 72 type 1 diabetic patients and 40 control individuals in Uruguay. This is a tri-racial (Caucasian, Black and Indo-American) mixed population. The products of the polymerase chain reaction amplifications were hybridized with oligonucleotides by allele-specific oligonucleotide reverse or dot blot methods. Significant differences between these two groups were observed only for allele DQB1*0302 (35%, RR = 7.34, P<0.001). The frequency of the alleles carrying a non-aspartic acid residue at position 57 was significantly higher in the diabetic patients (85 vs 53%, P<0.001). In contrast, the frequency of Asp alleles was negatively associated with type 1 diabetes (RR = 0.20, P<0.001). The genotype DQB1*0302/DQB1*0201 (33%, RR = 5.41, P<0.05) was positively associated with this disease. The genotype frequencies associated with type 1 diabetes in our population were significantly different from what is known for Caucasian and Black populations as well as compared with another admixed population, from Chile.
authors.affiliation: Cytogenetics Department, Associated Unit, Institute of Biology, School of Science, University of the Republic, Montevideo, Uruguay. abmg@iibce.edu.uy
authors.forename: Adriana
authors.initials: A
authors.lastname: Mimbacas
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Bravo
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Hidalgo
authors.forename: Gerardo
authors.initials: G
authors.lastname: Javiel
authors.forename: Carmen
authors.initials: C
authors.lastname: Pisciottano
authors.forename: Rosario
authors.initials: R
authors.lastname: Grignola
authors.forename: Ana María
authors.initials: AM
authors.lastname: Jorge
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Gallino
authors.forename: Jackeline
authors.initials: J
authors.lastname: Gasagoite
authors.forename: Horacio
authors.initials: H
authors.lastname: Cardoso
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D014583:Uruguay
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen