title: Ethanol intoxication and lactated Ringer's resuscitation prolong hemorrhage-induced lactic acidosis.
abstract: Ethanol (EtOH) blunts the respiratory and metabolic compensation during hemorrhage, resulting in a more severe lactic acidemia. We hypothesized that lactated Ringer's (LR) resuscitation may exacerbate this lactic acidemia. Male guinea pigs were implanted with arterial and venous catheters. Two days after catheter placement, conscious animals were injected intraperitoneally with 1 g/kg EtOH, 0.3 g/kg EtOH, or an equal volume of water 30 min before hemorrhage (60% of estimated blood volume). After 30 min of hemorrhagic shock, animals were resuscitated with isotonic saline (S) or LR at 1 mL/min (three times shed blood volume). Mean arterial blood pressure (MABP) was not affected by pretreatment with either dose of EtOH, but was significantly decreased by hemorrhage in all groups. Both S and LR resuscitation slightly increased MABP, but neither restored it to prehemorrhage values. Blood lactate levels increased in all groups during hemorrhage and remained elevated for 3 h in animals pretreated with 1 g/kg EtOH. In the group pretreated with 0.3 g/kg EtOH, pH decreased during shock but returned to prehemorrhage levels during the resuscitation period. Resuscitation with S returned pH to prehemorrhage values in animals pretreated with 1.0 g/kg EtOH. Resuscitation with LR did not exacerbate, but did prolong, the lactic acidemia after shock in animals pretreated with 1.0 g/kg EtOH. Administration of additional lactate during intoxication and hypovolemia for hemodynamic stabilization before blood transfusion may exacerbate a metabolic stress.
authors.affiliation: Department of Physiology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.
authors.forename: Albert N
authors.initials: AN
authors.lastname: Swafford
authors.forename: Dani
authors.initials: D
authors.lastname: Bidros
authors.forename: Terrence M
authors.initials: TM
authors.lastname: Truxillo
authors.forename: Mary E
authors.initials: ME
authors.lastname: Giaimo
authors.forename: Harvey I
authors.initials: HI
authors.lastname: Miller
authors.forename: Kathleen H
authors.initials: KH
authors.lastname: McDonough
mesh_terms: D000138:Acidosis / Q000473:pathology; D000140:Acidosis, Lactic / Q000473:pathology*; D000435:Alcoholic Intoxication; D000818:Animals; D001794:Blood Pressure; D002492:Central Nervous System Depressants / Q000506:poisoning / Q000633:toxicity; D000431:Ethanol / Q000097:blood / Q000506:poisoning*; D006168:Guinea Pigs; D006339:Heart Rate / Q000187:drug effects; D006470:Hemorrhage / Q000473:pathology*; D007552:Isotonic Solutions / Q000494:pharmacology*; D008297:Male; D012151:Resuscitation; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002492:Central Nervous System Depressants; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D000431:Ethanol; D012965:Sodium Chloride