Fluorescent and colored trinitrophenylated analogs of ATP and GTP.
Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleotide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visible region. Recent years have seen dramatic developments in the application of the TNP nucleotide analogs as spectroscopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants. This review is intended as a broad overview of currently extensively used applications of the nucleotide analogs in various biological systems.
Hiratsuka
Toshiaki
T
Department of Chemistry, Asahikawa Medical College, Japan. toshiaki@asahikawa-med.ac.jp
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D011485:Protein Binding; D014301:Trinitrobenzenes / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D008024:Ligands; D014301:Trinitrobenzenes; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate