title: Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pylori.
abstract: The triple-base-pair 16S rDNA mutation AGA(926-928)-->TTC mediates high-level tetracycline resistance in Helicobacter pylori. In contrast, single- and double-base-pair mutations mediated only low-level tetracycline resistance and decreased growth rates in the presence of tetracycline, explaining the preference for the TTC mutation in tetracycline-resistant H. pylori isolates.
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands.
authors.forename: Monique M
authors.initials: MM
authors.lastname: Gerrits
authors.forename: Marco
authors.initials: M
authors.lastname: Berning
authors.forename: Arnoud H M
authors.initials: AH
authors.lastname: Van Vliet
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Kuipers
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D003001:Cloning, Molecular; D017931:DNA Primers; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics* / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013753:Tetracycline Resistance / Q000235:genetics* / Q000502:physiology*
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S