Assessment of genetic variation in timothy (Phleum pratense L.) using RAPD and UP-PCR.
DNA-based fingerprinting technologies including random amplified polymorphic DNA (RAPD) and universally primed PCR (UP-PCR), a novel method for studying genetic variation, were employed as genetic markers for assessing genetic diversity and relationships in timothy (Phleum pratense L.). This study sought to identify the genetic background of the genotypes used in timothy breeding. Thirty eight genotypes from fifteen countries were used as test materials. RAPD and UP-PCR dendrograms based on 132 (from 3 primers) and 44 highly reproducible bands, respectively, were analyzed. The electrophoretic gels showed that the PCR products were informative and polymorphic. Different geographic genotype groups were distinguished according to the combined RADP and UP-PCR results. The results demonstrate that methods based on molecular fingerprinting can be used for timothy identification.
Guo
Yang-Dong
YD
Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, Turku, Finland. yaguo@affrc.go.jp
Yli-Mattila
Tapani
T
Pulli
Seppo
S
D001947:Breeding; D016172:DNA Fingerprinting; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D009697:Nucleolus Organizer Region; D031784:Phleum / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000145:classification*
D005819:Genetic Markers