Antioxidant and pro-oxidant effects of tannins in digestive cells of the freshwater mussel Unio tumidus.
Bivalve molluscs, particularly mussels, are sensitive biomarkers of aquatic ecosystem pollution. The tannins, water-soluble plant polyphenols, may play an important role in this environment and, mainly as a consequence of interaction with pollutants, their toxicity may change. We studied three naturally occurring compounds, tannic acid, ellagic acid and gallic acid, for their ability to modulate DNA damage produced by these tannins alone and in the presence of the oxidative stress inducer H(2)O(2), in cells of the digestive gland of mussels (Unio tumidus). After the treatment of the cells with polyphenols at different concentrations (1, 5, 15, 30, 60, 80, 100, 120, 180, 240 microM) and with hydrogen peroxide in the range of 0.04 and 0.1mM, single-strand breaks (ssb) in DNA were investigated, using the comet assay. The ability of phenolic acids to decrease DNA damage through their antioxidant properties was also assessed. The results show that the phenols, which are known as antioxidative agents, could also act as pro-oxidants. They induced ssb in DNA of the digestive gland at concentrations higher that 10 microM, but lower doses (1 and 5 microM) did not contribute to the DNA damage. This study was also designed to evaluate the protective effect of these tannins against H(2)O(2)-mediated DNA damage in the cells. In this treatment, the two concentrations (1 and 5 microM) significantly decreased the amount of lesions induced by H(2)O(2) (0.04 and 0.1mM). In conclusion, our results demonstrate that antioxidative properties of tannins may change to pro-oxidative activities at the higher concentrations. This suggests that the biologic actions of these compounds may be rather complicated.
Labieniec
Magdalena
M
Department of General Biophysics, University of Lodz, Banacha 12/16, Lodz 90-237, Poland.
Gabryelak
Teresa
T
Falcioni
Giancarlo
G
D000818:Animals; D000975:Antioxidants; D049872:Bivalvia; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004610:Ellagic Acid / Q000494:pharmacology; D005707:Gallic Acid / Q000494:pharmacology / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013634:Tannins / Q000494:pharmacology* / Q000633:toxicity*
D000975:Antioxidants; D047348:Hydrolyzable Tannins; D017382:Reactive Oxygen Species; D013634:Tannins; D004610:Ellagic Acid; D005707:Gallic Acid; D006861:Hydrogen Peroxide