Real-time PCR as a versatile tool for investigating the susceptibility of human herpesvirus 6 to antiviral agents.
A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.
Macé
Muriel
M
Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.
Manichanh
Chaysavanh
C
Bonnafous
Pascale
P
Précigout
Stéphanie
S
Boutolleau
David
D
Gautheret-Dejean
Agnès
A
Agut
Henri
H
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D005434:Flow Cytometry; D015654:Herpesvirus 6, Human / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral