title: Mouse blastocyst vitrification compared with the conventional slow-freezing method.
abstract: To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrification and slow-freezing were 68.33 and 65.52 per cent (p = 0.89), whereas hatching rates were 51.22 and 44.74 per cent, respectively (p = 0.64). Therefore, vitrification of blastocyst-stage-embryos may be a useful, economic method for freezing the excess blastocysts in some centers where blastocysts are routinely transferred.
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Tharnprisarn
authors.forename: S
authors.initials: S
authors.lastname: Suwajanakorn
authors.forename: W
authors.initials: W
authors.lastname: Sereepapong
authors.forename: K
authors.initials: K
authors.lastname: Pruksananonda
authors.forename: W
authors.initials: W
authors.lastname: Boonyakasemsanti
authors.forename: P
authors.initials: P
authors.lastname: Virutamasen
authors.forename: V
authors.initials: V
authors.lastname: Ahnonkitpanit
authors.forename: D
authors.initials: D
authors.lastname: Chompurat
authors.forename: P
authors.initials: P
authors.lastname: Numchaisrika
mesh_terms: D000818:Animals; D001755:Blastocyst; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013997:Time Factors