A model of calcium waves in pancreatic and parotid acinar cells.
We construct a mathematical model of Ca(2+) wave propagation in pancreatic and parotid acinar cells. Ca(2+) release is via inositol trisphosphate receptors and ryanodine receptors that are distributed heterogeneously through the cell. The apical and basal regions are separated by a region containing the mitochondria. In response to a whole-cell, homogeneous application of inositol trisphosphate (IP(3)), the model predicts that 1), at lower concentrations of IP(3), the intracellular waves in pancreatic cells begin in the apical region and are actively propagated across the basal region by Ca(2+) release through ryanodine receptors; 2), at higher [IP(3)], the waves in pancreatic and parotid cells are not true waves but rather apparent waves, formed as the result of sequential activation of inositol trisphosphate receptors in the apical and basal regions; 3), the differences in wave propagation in pancreatic and parotid cells can be explained in part by differences in inositol trisphosphate receptor density; 4), in pancreatic cells, increased Ca(2+) uptake by the mitochondria is capable of restricting Ca(2+) responses to the apical region, but that this happens only for a relatively narrow range of [IP(3)]; and 5), at higher [IP(3)], the apical and basal regions of the cell act as coupled Ca(2+) oscillators, with the basal region partially entrained to the apical region.
Sneyd
J
J
Department of Mathematics, University of Auckland, Auckland, New Zealand. sneyd@math.auckland.ac.nz
Tsaneva-Atanasova
K
K
Bruce
J I E
JI
Straub
S V
SV
Giovannucci
D R
DR
Yule
D I
DI
D000818:Animals; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology; D008954:Models, Biological; D008962:Models, Theoretical; D009991:Oscillometry; D010179:Pancreas / Q000166:cytology*; D010306:Parotid Gland / Q000166:cytology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D013997:Time Factors
D015220:Calcium Channels; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium