title: [Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry].
abstract: OBJECTIVE
  To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells.
  
  
  METHODS
  The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay.
  
  
  RESULTS
  The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro.
  
  
  CONCLUSIONS
  The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time dependent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.
authors.affiliation: Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Jun-long
authors.initials: JL
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Li
mesh_terms: D002692:Chlamydia trachomatis / Q000254:growth & development / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*
chemical_list: D016209:Interleukin-8; D016753:Interleukin-10