title: [Down-regulated full-length novel gene GDDR and its effect on gastric cancer].
abstract: OBJECTIVE
  To clone gene fragments from suppression substructive library established for screening down-regulated genes in gastric carcinoma, and obtain the full-length novel gene.
  
  
  METHODS
  Gene fragments were identified by sequencing plasmids of positive colonies chosen randomly. One gene fragment was amplified by RACE, and the full-length novel gene was obtained. Expression of novel gene mRNA was respectively detected by semi-quantitative PCR in the gastric carcinoma tissues and counterpart normal gastric mucous membrane of 25 patients with gastric cancer. The structure of the full-length novel gene, location on chromosome, property of protein encoded by full-length novel gene and its function were investigated by Bio-message technique.
  
  
  RESULTS
  One 331 bp gene fragment was cloned, and its full-length novel gene obtained by RACE. The novel full-length gene was named GDDR, registered in the number of AF494509 by GenBank. Significant down-regulated expression of GDDR gene mRNA in gastric carcinoma tissues was confirmed (GDDR/beta-actin 13.474 +/- 5.059 vs 1.041 +/- 0.202, P < 0.01). GDDR was located in chromosome 2p13 with 5 exons. As one member of new BRICHO family as CA11, GDDR encoding protein with transmembrane peptide revealed homology to protein encoded by CA11.
  
  
  CONCLUSIONS
  A novel full-length gene GDDR is obtained. GDDR likely is another gene of BRICHO family related to gastric cancer.
authors.affiliation: Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xian 710032, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Du
authors.forename: Ke-feng
authors.initials: KF
authors.lastname: Dou
authors.forename: Shu-you
authors.initials: SY
authors.lastname: Peng
authors.forename: Wei-zhong
authors.initials: WZ
authors.lastname: Wang
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Hua-sheng
authors.initials: HS
authors.lastname: Xiao
authors.forename: Wen-xian
authors.initials: WX
authors.lastname: Guan
authors.forename: Ying-bin
authors.initials: YB
authors.lastname: Liu
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; C503073:GKN2 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger