title: Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry.
abstract: Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and propidium iodide (PI) while the second protocol added the mitochondrial probe JC-1. The relationship between total sperm motility and percent viable sperm was high between staining protocols (r = 0.98). Time (0 h versus 24 h, P<0.0001) and treatment (0, 10, 25, 50, and 75% nonviable sperm, P<0.0001) affected percent total sperm motility and percent viable sperm for both staining protocols. Actual percent viable sperm for each time and treatment did not differ from expected values.
authors.affiliation: Department of Physiology and Pharmacology, Texas A&M University, College of Veterinary Medicine, College Station, TX 77843-4475, USA. clove@cvm.tamu.edu
authors.forename: C C
authors.initials: CC
authors.lastname: Love
authors.forename: J A
authors.initials: JA
authors.lastname: Thompson
authors.forename: S P
authors.initials: SP
authors.lastname: Brinsko
authors.forename: S L
authors.initials: SL
authors.lastname: Rigby
authors.forename: T L
authors.initials: TL
authors.lastname: Blanchard
authors.forename: V K
authors.initials: VK
authors.lastname: Lowry
authors.forename: D D
authors.initials: DD
authors.lastname: Varner
mesh_terms: D000818:Animals; D001562:Benzimidazoles; D002232:Carbocyanines; D002470:Cell Survival; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006736:Horses / Q000502:physiology*; D008297:Male; D009930:Organic Chemicals; D011419:Propidium; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C093207:SYBR-14; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D011419:Propidium