Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells.
We examined the intracellular Ca(2+) response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca(2+) with a characteristic lag phase. The cytoplasmic free Ca(2+) increase was independent of extracellular Ca(2+), but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP(3)) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca(2+) through a phospholipase C-mediated pathway, and that the release of Ca(2+) from intracellular stores mainly contributes to the increase in cytoplasmic free Ca(2+). Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca(2+) mobilization.
Zhai
Haifeng
H
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, 180 Yamashiro-Cho, 770-8514 Tokushima, Japan.
Nakade
Kousuke
K
Mitsumoto
Yasuhide
Y
Fukuyama
Yoshiyasu
Y
D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D017705:Lignans; D009447:Neuroblastoma / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D002118:Calcium; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols