[Relationship between expressions of GATA-3 and interleukin-5 in nasal polyps].
OBJECTIVE
To evaluate the role of transcription factor GATA-3 in the pathogenesis of nasal polyps.


METHODS
The expression of GATA-3 was detected by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in 28 cases of nasal polyps and 17 specimens of normal nasal epithelium. The expression of interleukin-5 (IL-5) in these specimens was detected by enzyme-linked immunosorbent assay (ELISA) meanwhile.


RESULTS
Immunohistochemistry showed, as confirmed by RT-PCR, a positive rate of GATA-3 of 89.3% in the nasal polyp (25/28) and 29.4% in the normal nasal mucosa (5/17), P < 0.05. GATA-3 was mainly distributed in submucous inflammatory cells. The relative density ratio of GATA-3 to GAPDH was 0.618 +/- 0.137 in nasal polyp and 0.21 +/- 0.11 in normal nasal mucosa (P < 0.05) as indicated by RT-PCR and agarose electrophoresis. The concentrations of IL-5 were 69.4 +/- 15.1 pg/ml and 25.7 +/- 13.0 pg/mg in the two groups respectively. The expression of GATA-3 was positively correlated to the expression of IL-5 in the nasal polyp.


CONCLUSIONS
Contributing to the overexpression of such cytokines as IL-5, GATA-3 may play an important role in the pathogenesis of nasal polyps.
Li
Hua-bin
HB
Beijing Institute of Otolaryngology, Department of Otolaryngology and Head and Neck Surgery, Tongren Hospital, Capital Medical University, Beijing 100005 China.
Han
De-min
DM
Zhou
Bing
B
Zhu
Xiao-nong
XN
Liu
Zhong-yan
ZY
D000293:Adolescent; D000328:Adult; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D050990:GATA3 Transcription Factor; D006801:Humans; D007150:Immunohistochemistry; D015848:Interleukin-5 / Q000032:analysis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000209:etiology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000032:analysis* / Q000235:genetics
D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D015848:Interleukin-5; D012333:RNA, Messenger; D015534:Trans-Activators