title: Multiple HPV infection in cervical cancer screened by HPVDNAChip.
abstract: This study determined the distribution of high-risk HPV type infection in cervical cancer using newly developed oligonucleotide chips (HPVDNAChips). The study subjects included 80 cases of cervical neoplasia and 746 controls with a normal Pap smear. For HPV genotyping, the commercially available HPVDNAChips was used. The risk of cervical cancer was increased in women with a family history of cervical cancer (adjusted OR=2.3, 95% CI: 0.92-6.17) and in smokers (adjusted OR=3.2, 95% CI: 1.45-7.06). There was also a trend of increased risk with the number of full term pregnancies (P(for trend)<0.001). There were only 7.2% (54 of 746) infected high-risk HPV types in the control, whereas 54.5% (six of 11) and 76.5% (52 of 68) were infected in the CIN and cervical cancer, respectively. Multiple HPV infection was observed in 0.5% (three of 592) of the control group but in 9.1% (seven of 77) of cases. Multivariate analysis revealed that subjects infected with multiple HPV types had a 31.8-fold (95% CI: 7.50-134.75) higher risk of cervical cancer, while the single HPV type had a 19.9-fold increased risk (95% CI: 10.90-36.18) (P(for trend)<0.001). These results show that the detection and typing of HPV infection by HPVDNAChip can be a useful in clinical applications because it provides information on multiple infections and the types of HPV in addition to HPV infection status.
authors.affiliation: Department of Preventive Medicine, College of Medicine, Seoul National University, 28 Yongun-dong, Chongno-gu, Seoul 110-799, South Korea.
authors.forename: Sang Ah
authors.initials: SA
authors.lastname: Lee
authors.forename: Daehee
authors.initials: D
authors.lastname: Kang
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Seo
authors.forename: Jeongmi Kim
authors.initials: JK
authors.lastname: Jeong
authors.forename: Keun Young
authors.initials: KY
authors.lastname: Yoo
authors.forename: Yong Tark
authors.initials: YT
authors.lastname: Jeon
authors.forename: Jae Weon
authors.initials: JW
authors.lastname: Kim
authors.forename: Noh Hyun
authors.initials: NH
authors.lastname: Park
authors.forename: Soon Beom
authors.initials: SB
authors.lastname: Kang
authors.forename: Hyo Pyo
authors.initials: HP
authors.lastname: Lee
authors.forename: Yong Sang
authors.initials: YS
authors.lastname: Song
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005190:Family; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D010298:Parity; D012016:Reference Values; D012907:Smoking; D011795:Surveys and Questionnaires; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral