title: Determination of hemoglobin adducts formed in rats exposed orally with 3,3'-dichlorobenzidine by GC/MS-SIM.
abstract: 3,3'-dichlorobenzidine (DCB) can be metabolically N-acetylated and/or N-oxidized, and can form hemoglobin adducts. Gas chromatography/mass spectrometry-selected ion monitoring detection mode (GC/MS-SIM) could be a good analytical method to detect them. 4-Aminobiphenyl and phenanthrene-d10 were used as internal standards, and standard metabolites of DCB were synthesized from DCB. Pyridine is a promoter and acetic acid is a controller in the acetylation of DCB during titrating with acetyl chloride. After washing with acetone, the purity of N-acetyl DCB and N,N'-diacetyl DCB were 98.72% and 98.82%, respectively. The maximum detection limits (MDLs) by GC/MS-SIM were 0.5 microg/L in DNA adduct and 1.0 ng/g in hemoglobin for DCB and N-acetyl DCB. The base peaks of their fragmentation pattern were 252 m/z at the peak of DCB, 252 m/z and 294 m/z at the peak of N-acetyl DCB, and 252 m/z, 294 m/z, and 336 m/z at the peak of N,N'-diacetyl DCB. This analytical method was applied to determine hemoglobin adducts formed in young female Sprague-Dawley rats orally exposed with 20, 30, and 40 mg DCB/kg/day for three weeks. Two adducts were detectable by GC/MS-SIM after alkaline hydrolysis of hemoglobin samples and extraction. The structure of these adducts could be assigned to DCB and N-acetyl DCB by co-chromatography with the synthetic standards. After the first week of treatment, the total amount of hemoglobin adducts determined was 837.5 approximately 2501 ng/g hemoglobin. The adduct levels were increased up to 1203.3 approximately 2605.4 ng/g after the second week, and slightly decreased after the third week. The ratio of DCB and N-acetyl DCB was nearly similar in all treatment groups at the third week, such as 4.28 approximately 4.78. Three different treatments (20, 30, and 40 mg DCB/kg) of rats resulted in dose-proportional increases in the total and DCB amount of hemoglobin adducted formed for three weeks. The relative contribution of DCB and N-acetyl DCB to the total hemoglobin adduct level was strongly dose dependent. The results show that GC/ MS-SIM is suitable for the biological monitoring of humans exposed to DCB or DCB-containing products.
authors.affiliation: Department of Environmental Education, Abuse Drug Research Center, Kongju National University, Kongju 314-701, South Korea. ejhl@kongju.ac.kr
authors.forename: Jin Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
mesh_terms: D015101:3,3'-Dichlorobenzidine / Q000494:pharmacology / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D002273:Carcinogens; D006454:Hemoglobins; D015101:3,3'-Dichlorobenzidine