In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase.
The influence of an extremely low frequency (ELF) magnetic field (50 Hz and 1 mT, EMF) on the activity of a soluble and insoluble horseradish peroxidase (E.C. 1.11.17) has been studied as a function of time. Insoluble derivatives were obtained by enzyme entrapment into two different gelatin membranes or by covalent attachment of the enzyme on two nylon membranes, differently preactivated. Results have shown that the field affects the inactivation rate of the soluble enzyme, while no effects are observed with insoluble derivatives. Since in vivo enzymes are immobilised into the biomembrane bilayer or entrapped into the cytoplasmic mixture, one might speculate that our experimental conditions do not reflect the catalytic activity of the enzymes in vivo.
Portaccio
M
M
Department of Experimental Medicine, Faculty of Medicine and Surgery, Second University of Naples, Naples, Italy.
De Luca
P
P
Durante
D
D
Rossi
S
S
Bencivenga
U
U
Canciglia
P
P
Lepore
M
M
Mattei
A
A
De Maio
A
A
Mita
D G
DG
D052160:Benzothiazoles; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D004789:Enzyme Activation / Q000528:radiation effects*; D004795:Enzyme Stability / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000528:radiation effects*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008567:Membranes, Artificial; D011846:Radio Waves; D012995:Solubility; D012996:Solutions; D013451:Sulfonic Acids / Q000737:chemistry
D052160:Benzothiazoles; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D012996:Solutions; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase