2-D array formation of genetically engineered viral cages on au surfaces and imaging by atomic force microscopy.
The preparation and subsequent imaging of a two-dimensional array of a genetically and chemically modified cowpea chlorotic mottle virus (CCMV) is described. The genetic mutation provides symmetrically dispersed exposed thiol groups on the outer surface of the virus capsid. These functional groups can be used to covalently bind the capsid to smooth Au substrate. AFM imaging suggests that the genetic mutation by itself does not promote array formation but, rather, aggregation through disulfide linkages. However, breaking the symmetry of the capsid using a solid-phase approach and chemically passivating the exposed thiol groups with iodoacetic acid results in a capsid with exposed thiols only on one side of the particle. These symmetry-broken capsids were able to form self-assembled monolayers (SAM) on a Au surface.
Klem
Michael T
MT
Department of Chemistry & Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
Willits
Debbie
D
Young
Mark
M
Douglas
Trevor
T
D000409:Alanine / Q000737:chemistry / Q000235:genetics; D019943:Amino Acid Substitution; D017795:Bromovirus / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D014771:Virion / Q000737:chemistry / Q000235:genetics
D036022:Capsid Proteins; D006046:Gold; D003545:Cysteine; D000409:Alanine