Molecular differentiation of Panax species by RAPD analysis.
Traditional taxonomic methods used for the identification and differentiation of ginsengs rely primarily on morphological observations or physiochemical methods, which cannot be used efficiently when only powdered forms or shredded material is available. Randomly amplified polymorphic DNA (RAPD) was used to determine the unique DNA profiles that are characteristic not only of the genus Panax but also of various Panax subgroups collected from five different countries. RAPD results of OP-5A primer showed a specific single band that is characteristic of all ginseng samples. RAPD results of OP-13B primer demonstrated that OP-13B primer could be used as a unique RAPD marker to differentiate Panax species. These results support that this approach could be applied to distinguish Korean Ginseng (Panax ginseng) from others at the molecular level.
Shim
Young-Hun
YH
College of Pharmacy, Chung Ang University, 221, Huksuk-Dong, Dongjak-Ku, Seoul 156-756, Korea.
Choi
Jung-Ho
JH
Park
Chan-Dong
CD
Lim
Chul-Joo
CJ
Cho
Jung-Hee
JH
Kim
Hong-Jin
HJ
D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005894:Panax / Q000737:chemistry / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D018744:DNA, Plant