Influence of inocula and grains on sclerotia biomass and carotenoid yield of Penicillium sp. PT95 during solid-state fermentation.
Various inocula and grains were evaluated for carotenoid production by solid-state fermentation using Penicillium sp. PT95. Millet medium was more effective in both sclerotia growth and carotenoid production than other grain media. An inoculum in the form of sclerotia yielded higher sclerotia biomass compared to either a spore inoculum or a mycelial pellet inoculum. Adding wheat bran to grain medium favored the formation of sclerotia. However, neither the inoculum type nor addition of wheat bran resulted in a significant change in the carotenoid content of sclerotia. Among grain media supplemented with wheat bran (wheat bran:grain =1:4 w/w, dry basis), a medium consisting of rice and wheat bran gave the highest sclerotia biomass (15.10 g/100 g grain), a medium consisting of buckwheat and wheat bran gave the highest content of carotenoid in sclerotia (0.826 mg/g dry sclerotia), and a medium consisting of millet and wheat bran gave the highest carotenoid yield (11.457 mg/100 g grain).
Han
Jian-Rong
JR
Institute of Biotechnology, Shanxi University, 030006, Taiyuan, People's Republic of China. hjr@sxu.edu.cn
Yuan
Jing-Ming
JM
D002338:Carotenoids / Q000096:biosynthesis*; D003470:Culture Media; D004043:Dietary Fiber; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D025282:Mycelium / Q000254:growth & development; D012275:Oryza; D008897:Panicum; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism*; D013171:Spores, Bacterial / Q000254:growth & development; D003313:Zea mays
D003470:Culture Media; D004043:Dietary Fiber; D002338:Carotenoids