title: Evaluation of anti-inflammatory activity of chloroform extract of Bryonia laciniosa in experimental animal models.
abstract: The anti-inflammatory effect of the leaves of Bryonia laciniosa was evaluated using carrageenan, dextran, histamine, serotonin induced rat paw oedema and cotton pellet induced granuloma (chronic) models in rats. In mice, carrageenan peritonitis test was performed for the extract by oral administration. The chloroform extract of Bryonia laciniosa (CEBL) exhibited significant anti-inflammatory effect at the dose 50, 100 and 200 mg/kg. Maximum inhibition (52.4%) was noted at the dose of 200 mg/kg after 3 h of drug treatment in carrageenan induced paw oedema, whereas the indomethacin (standard drug) produced 62.1% of inhibition. The extract exhibited significant anti-inflammatory activity in dextran induced paw oedema in a dose dependent manner. The extract also exhibited significant inhibition on the hind paw oedema in rats caused by histamine and serotonin respectively. In the chronic model (cotton pellet induced granuloma) the CEBL (200 mg/kg) and standard drug showed decreased formation of granuloma tissue by 50.1 and 57.3% (p<0.001) respectively. The extract also inhibited peritoneal leukocyte migration in mice. Thus, the present study revealed that the chloroform extract of Bryonia laciniosa exhibited significant anti-inflammatory activity in the tested models.
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India. sivaecp@hotmail.com
authors.forename: Malaya
authors.initials: M
authors.lastname: Gupta
authors.forename: Upal Kanti
authors.initials: UK
authors.lastname: Mazumdar
authors.forename: Thangavel
authors.initials: T
authors.lastname: Sivakumar
authors.forename: Madgulav Laxmi Mohan
authors.initials: ML
authors.lastname: Vamsi
authors.forename: Subhas Somalingappa
authors.initials: SS
authors.lastname: Karki
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Sambathkumar
authors.forename: Laxmanan
authors.initials: L
authors.lastname: Manikandan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000633:toxicity; D029772:Bryonia / Q000737:chemistry*; D002351:Carrageenan; D002725:Chloroform; D003911:Dextrans; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005122:Exudates and Transudates / Q000187:drug effects; D005260:Female; D006099:Granuloma / Q000139:chemically induced / Q000517:prevention & control; D006632:Histamine / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D012997:Solvents
chemical_list: D000893:Anti-Inflammatory Agents; D003911:Dextrans; D018836:Inflammation Mediators; D010936:Plant Extracts; D012997:Solvents; D012701:Serotonin; D002725:Chloroform; D006632:Histamine; D002351:Carrageenan; D007213:Indomethacin