In vitro demonstration of the hemolytic, cytotoxic activities and induction of apoptosis in Orientia tsutsugamushi infected L929 mouse fibroblast cells.
Using cultured mouse fibroblast L929 cells, this study demonstrated the hemolytic and cytotoxic activities and induction of apoptosis in cells infected with Orientia tsutsugamushi. Low levels of hemolytic activity were detected using heavily infected cells. No hemolysin or cytotoxin were detected in the infected culture fluid regardless of the pathogenicity of the O. tsutsugamushi strains in mice. Using propidium iodide uptake assay, acridine orange/ethidium bromide staining and terminal deoxynucleotide transferase-mediated dUTP-digoxigenin nick-end labeling assay, apoptosis was observed in L929 cells infected with Karp and Gilliam strains.
Tay
S T
ST
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Rohani
M Y
MY
Ho
T M
TM
Devi
S
S
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D004396:Coloring Agents; D006461:Hemolysis; D066298:In Vitro Techniques; D051379:Mice; D012285:Orientia tsutsugamushi / Q000472:pathogenicity*; D012756:Sheep; D037521:Virulence Factors
D004396:Coloring Agents; D037521:Virulence Factors