title: Hepatic CYP1A levels and EROD activity in English sole: biomonitoring of marine contaminants in Vancouver Harbour.
abstract: To assess chemical contaminant stress in the marine environment, ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) expression were measured in 88 English Sole (Pleuronectes vetulus) collected during May and June 1999 from four sites in Vancouver Harbour and at an expected reference site outside the harbour. Hepatic microsomes were prepared from the fish and analyzed for total CYP content, EROD activity, and CYP1A protein levels. Hepatic EROD activity and CYP1A protein levels were elevated in fish from two sites in the inner harbour. A comparison with sediment chemistry data showed that fish with increased EROD activity and CYP1A levels came from sites containing relatively high levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls. Unexpectedly high levels of EROD activity and CYP1A protein were also found in fish from a reference site near Gibsons, in Howe Sound. The elevated EROD activity and CYP1A expression in fish from this site cannot be explained by the chemical analysis data collected.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of British Columbia, 2146 East Mall, Vancouver, British Columbia, Canada V6T 1Z3.
authors.forename: K A
authors.initials: KA
authors.lastname: Miller
authors.forename: R F
authors.initials: RF
authors.lastname: Addison
authors.forename: S M
authors.initials: SM
authors.lastname: Bandiera
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000494:pharmacology*; D004784:Environmental Monitoring / Q000379:methods; D005432:Flounder / Q000502:physiology*; D019015:Geologic Sediments / Q000737:chemistry; D006838:Hydrocarbons / Q000506:poisoning*; D010578:Petroleum; D014873:Water Pollutants / Q000506:poisoning*
chemical_list: D015415:Biomarkers; D006838:Hydrocarbons; D010578:Petroleum; D014873:Water Pollutants; D019363:Cytochrome P-450 CYP1A1