Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method and a dual-round polymerase chain reaction.
Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.
Iwasa
Mineo
M
Department of Forensic Medical Science, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-ku, Japan. nagaom@med.nagoya-cu.ac.jp
Koyama
Hiroyoshi
H
Tsuchimochi
Tsukasa
T
Maeno
Yoshitaka
Y
Isobe
Ichiro
I
Seko-Nakamura
Yoshimi
Y
Monma-Ohtaki
Jun
J
Matsumoto
Tomohiro
T
Nagao
Masataka
M
D000328:Adult; D002614:Chelating Agents; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology; D020080:Tandem Repeat Sequences; D014621:Vagina / Q000166:cytology; D014626:Vaginal Smears
D002614:Chelating Agents; D004247:DNA