Multiprotein signaling complexes and regulation of cardiac phenotype.
There is increasing evidence across disciplines for multiprotein complexes as a mechanism for signal transduction in various cell types. These multiprotein complexes appear to be altered as a direct mechanism to confer signaling responses and to alter phenotype. Although classical experimental techniques are effective to delineate the signaling role of one or a few molecules, they are limited in their ability to thoroughly characterize multiprotein complexes. In this review, the contribution of functional proteomics to a protein complex-based portrait of cardiac cell signaling will be examined along with a discussion and brief evaluation of the state-of-the-art proteomic approaches for multiprotein complex analysis.
Vondriska
Thomas M
TM
Department of Physiology, Division of Cardiology, Cardiovascular Research Laboratory, David Geffen School of Medicine at University of California, Suite 1609 MRL Building, Los Angeles, 675 C.E. Young Drive, Los Angeles, CA 90095-1760, USA.
Ping
Peipei
P
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D046911:Macromolecular Substances; D008954:Models, Biological; D046912:Multiprotein Complexes; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D010641:Phenotype; D011493:Protein Kinase C / Q000032:analysis; D051744:Protein Kinase C-epsilon; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D015398:Signal Transduction; D019061:src-Family Kinases / Q000378:metabolism
D015415:Biomarkers; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D020543:Proteome; D019061:src-Family Kinases; C497255:PRKCE protein, human; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon