Differential modulation of interleukin-6 expression by interleukin-1beta in neuronal and glial cultures.
We analysed the specific effects of IL-1beta immunoneutralization on the expression of IL-6 in different pure cultures of neurones and glia after both experimental subliminal hypoxia and recovery. Whereas the IL-1beta-deprivation signal induced a decrease in IL-6 expression and release of normoxic neurones, it provoked an increase in IL-6 protein in hypoxic neurones. Moreover, the direct correlation between IL-1beta and IL-6, observed in normal and recovering neuronal cultures, was reversed in hypoxic conditions. These reversals were not observed in glial cells, in which IL-1beta immunosuppression led to a decrease in IL-6 under all conditions considered. In conclusion, the IL-1beta modulates IL-6 in different ways according to the ambient physiological or pathological conditions, and also acts via different mechanisms, depending on the cellular phenotype.
Di Loreto
Silvia
S
Istituto Trapianti díOrgano e Immunocitologia, CNR, L'Aquila, Italy. s.diloreto@area.ba.cnr.it
Maccarone
Rita
R
Corvetti
Luigi
L
Sebastiani
Pierluigi
P
Piancatelli
Daniela
D
Adorno
Domenico
D
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000502:physiology*; D015850:Interleukin-6 / Q000235:genetics*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007375:Interleukin-1; D015850:Interleukin-6