[Whole amount in situ hybridization and transgene via microinjection in zebrafish].
Zebrafish is new model organism to study development of the vertebrate. In this report, we chose a fragment of gene GATA-1 which specifically expressed in zebrafish hematopoiesis system as probe to carry on whole amount in situ hybridization. Then we transported reporter gene driven by GATA-1 promoter into the embryos via microinjection approach and observed the spatial and temporal expression pattern of GFP. Our results demonstrated that efficient and reliable technology of whole amount in situ hybridization and microinjection in zebrafish was established.
Yang
Xue Yan
XY
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China.
Yao
Ji Hua
JH
Cheng
Lu
L
Wei
Dong Wang
DW
Lu
Da Ru
DR
Xue
Jing Lun
JL
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D017403:In Situ Hybridization; D008845:Microinjections; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D019076:Transgenes / Q000235:genetics; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
D018076:DNA, Complementary; D011994:Recombinant Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins