Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis.
We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.
Fitzgerald
Anna M
AM
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Mudge
Agnieszka M
AM
Gleave
Andrew P
AP
Plummer
Kim M
KM
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development*; D005822:Genetic Vectors; D025301:Hyphae / Q000254:growth & development; D008938:Mitosis; D025282:Mycelium / Q000254:growth & development; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011523:Protoplasts; D013172:Spores, Fungal / Q000254:growth & development; D014170:Transformation, Genetic / Q000187:drug effects
D011092:Polyethylene Glycols