title: Increased seroreactivity in tic disorder patients to a 60 kDa protein band from a neuronal cell line.
abstract: In tic disorders, increased seroreactivity against neuronal antigens has been demonstrated, without performing molecular characterization of antigens. Here, unselected patients with a tic disorder were compared with healthy controls, autistic disorder (AD), and obsessive-compulsive disorder (OCD) patients. Seroreactivity against neuroblastoma cells was analyzed by Western blot. Anti-60 kDa binding occurred significantly more frequently in tic disorder patients (67.1%) than in AD (40.0%), OCD (40.0%) and healthy controls (41.9%). Sequence analysis of the 60 kDa protein band identified this as a ubiquitous heat shock protein. However, the involvement of other autoantigens with a molecular weight of 60 kDa cannot be excluded.
authors.affiliation: Child and Adolescent Psychiatry Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. Pieter.Hoekstra@kjpnn.nl
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Hoekstra
authors.forename: Gerda
authors.initials: G
authors.lastname: Horst
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Limburg
authors.forename: Pieter W
authors.initials: PW
authors.lastname: Troost
authors.forename: Natasja
authors.initials: N
authors.lastname: van Lang
authors.forename: Annelies
authors.initials: A
authors.lastname: de Bildt
authors.forename: Jakob
authors.initials: J
authors.lastname: Korf
authors.forename: Cees G M
authors.initials: CG
authors.lastname: Kallenberg
authors.forename: Ruud B
authors.initials: RB
authors.lastname: Minderaa
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000276:immunology* / Q000473:pathology; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D012720:Severity of Illness Index; D013981:Tic Disorders / Q000097:blood / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D018834:Chaperonin 60; D007074:Immunoglobulin G; D010446:Peptide Fragments