Radiation-induced apoptosis in scid mice spleen after low dose irradiation.
To assess the radioadaptive response of the whole body system in mice, we examined the temporal effect of low dose priming as an indicator of challenging irradiation-induced apoptosis through a p53 tumor suppressor protein- mediated signal transduction pathway. The p53 protein also plays an important role both in cell cycle control and DNA repair through cellular signal transduction. Using severe combined immunodeficiency mice defective in DNA-dependent protein kinase catalytic subunit, we examined the role of DNA-dependent protein kinase activity in radioadaptation induced by low dose irradiation. Specific pathogen free 5-week-old female severe combined immunodeficiency mice and the parental mice (CB- 17 Icr +/+) were irradiated with X-ray at 3.0 Gy at 1, 2, 3 or 4 weeks after the conditioning irradiation at 0.15, 0.30, 0.45 or 0.60 Gy. The mice spleens were fixed for immunohistochemistry 12 h after the challenging irradiation. The p53-dependent apoptosis related Bax proteins on formalin-fixed paraffin-embedded sections were stained by the avidin-biotin peroxidase complex method. The apoptosis incidence in the sections was measured by hematoxylin-eosin staining. The frequency of Bax- and apoptosis-positive cells increased up to 12 h after the challenging irradiation in the spleen of both mice. However, these cells were not observed after a low dose irradiation at 0.15-0.60 Gy. When pre-irradiation at 0.45 Gy 2 weeks before the challenging irradiation at 3.0 Gy was performed, Bax accumulation and apoptosis induced by challenging irradiation were depressed in the spleens of CB-17 Icr +/+ mice, but not in severe combined immunodeficiency mice. These data suggest that DNA-dependent protein kinase might play a major role in radioadaptation induced by pre-irradiation with a low dose in mice spleen. We expect that the present findings will provide useful information in the health care of space crews.
Takahashi
A
A
Department of Biology, Nara Medical University, Kashihara, Nara, Japan.
Kondo
N
N
Inaba
H
H
Uotani
K
K
Kiyohara
Y
Y
Ohnishi
K
K
Ohnishi
T
T
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle / Q000528:radiation effects; D004260:DNA Repair; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency / Q000378:metabolism / Q000528:radiation effects*; D011518:Proto-Oncogene Proteins / Q000378:metabolism / Q000528:radiation effects*; D019253:Proto-Oncogene Proteins c-bcl-2; D011829:Radiation Dosage; D011836:Radiation Tolerance; D016511:Severe Combined Immunodeficiency / Q000201:enzymology / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects*; D013154:Spleen / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000528:radiation effects; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D051747:DNA-Activated Protein Kinase; D017346:Protein Serine-Threonine Kinases