Inhibition of beta-fructofuranosidases and alpha-glucosidases by synthetic thio-fructofuranoside.
A synthetic beta-thio-fructofuranoside of mercaptoethanol inhibited not only beta-fructofuranosidases but also alpha-glucosidases. The compound was hardly hydrolyzed by the glycosidases. The thio-fructoside competitively inhibited beta-fructofuranosidases from Aspergillus niger, Candida sp., and Saccharomyces cerevisiae, but not Arthrobacter beta-fructofuranosidase at all. Sucrase activity of rat intestinal sucrase/isomaltase complex was also suppressed in the presence of the thio-fructoside. The thio-fructoside showed noncompetitive inhibition toward maltase activity of the rat intestinal enzyme complex and Saccharomyces sp. alpha-glucosidase. Inhibition against the Bacillus stearothermophilus alpha-glucosidase, Rhizopus glucoamylase, and porcine kidney trehalase were more slight than that against these two alpha-glucosidases.
Kiso
Taro
T
Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan. tkiso@omtri.city.osaka.jp
Hamayasu
Kenichi
K
Fujita
Koki
K
Hara
Kozo
K
Kitahata
Sumio
S
Nakano
Hirofumi
H
D000818:Animals; D001173:Arthrobacter / Q000201:enzymology; D001234:Aspergillus niger / Q000201:enzymology; D001667:Binding, Competitive; D002175:Candida / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005632:Fructose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D006868:Hydrolysis; D007422:Intestines / Q000201:enzymology; D007700:Kinetics; D008320:Maltose / Q000378:metabolism; D051381:Rats; D012233:Rhizopus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013393:Sucrase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013552:Swine; D014198:Trehalase / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D013440:Sulfides; D005632:Fructose; D013395:Sucrose; D008320:Maltose; D043324:beta-Fructofuranosidase; D014198:Trehalase; D005087:Glucan 1,4-alpha-Glucosidase; D013393:Sucrase