Semiquantitative RT-PCR method coupled to capillary electrophoresis to study 5alpha-reductase mRNA isozymes in rat ventral prostate in different androgen status.
The development and growth of the prostate gland depends on androgen stimulation. Dihydrotestosterone (DHT) is the primary androgen responsible for prostate development and also for the pathogenesis of benign prostatic hyperplasia (BPH). The incidence of prostate cancer (PCa) and benign prostatic hypertrophy (BPH) continues to rise in the Western world. DHT is synthesized in prostate from circulating testosterone (T) through the action of 5alpha-Reductase (5alpha-R) (EC 1.3.99.5), which occurs as two isozymes, type 1 and type 2. Type-1 5alpha-R is widely distributed in the body, and type-2 5alpha-R is confined to androgen-dependent structures. Both types are expressed in the prostate: type-2 isozyme is implicated in BPH and PCa; type-1 isozyme is also increased in some prostatic adenocarcinomas. In recent years, various inhibitors of type-2 isozyme or of both type-1 and type-2 isozyme have been used in prostatic diseases. In this work we present measurements of mRNA levels of steroid 5alpha-R isozymes in the ventral prostate of rats of different androgen status. We used a novel method that combines the high specificity of semiquantitive PCR with the sensitivity of laser-induced fluorescence capillary electrophoresis (LIF-CE). We demonstrated that T control the expression of 5alpha-R2 isozyme in rat prostrate. This approach could be of great value for the study of prostate diseases in humans and would allow study at the transcriptional level the effects of drugs that inhibit either or both of these isozymes.
Torres
Jesus M
JM
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain.
Gómez-Capilla
José A
JA
Ruiz
Estrella
E
Ortega
Esperanza
E
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D042944:Cholestenone 5 alpha-Reductase / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D008297:Male; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000201:enzymology*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013739:Testosterone / Q000378:metabolism; D014158:Transcription, Genetic
D000728:Androgens; D009841:Oligonucleotides; D020033:Protein Isoforms; D012333:RNA, Messenger; D013739:Testosterone; D042944:Cholestenone 5 alpha-Reductase