Analysis of tobacco-specific N-nitrosamines in snuff by ethyl acetate extraction and liquid chromatography-tandem mass spectrometry.
A rapid, selective and sensitive method for routine analysis of the four tobacco-specific N-nitrosamines, N'-nitrosonornicotine, N'-nitrosoanatabine, N'-nitrosoanabasine and 4-(methyl-nitrosamino)-1-(3-pyridyl)-1-butanone in snuff has been developed. The nitrosamines were isolated by ethyl acetate extraction and analysed by LC-MS-MS. Except for evaporation and filtration, no additional clean-up steps are needed in the proposed method. The detection limits for standard in solvent are between 0.0005 and 0.001 microg/ml (0.005 and 0.01 microg/g).
Jansson
Christer
C
Swedish National Food Administration, Research and Development Department, Box 622, SE-751 26 Uppsala, Sweden. chja@slv.se
Paccou
Alexandre
A
Osterdahl
Bengt-Göran
BG
D000085:Acetates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009602:Nitrosamines / Q000032:analysis*; D012680:Sensitivity and Specificity; D014030:Tobacco, Smokeless / Q000737:chemistry*
D000085:Acetates; D009602:Nitrosamines; C007650:ethyl acetate