Disodium cromoglycate prevents ileum hyperreactivity to histamine in Toxocara canis-infected guinea pigs.
The aim of this study was to investigate whether Toxocara canis infection in guinea pigs provokes changes in ileum responsiveness to histamine. Ileum segments from control and T. canis-infected groups were placed at isometric conditions and submitted to various doses of histamine. No changes were observed between controls and T. canis-infected groups at days 3, 6 and 12 after infection. However, at days 18 and 24 after infection, there was a significant increase in ileum responsiveness to histamine in T. canis-infected group. Pre-incubation of ileum segments with 1mgml(-1) disodium cromoglycate (DSCG) prevented the increased responsiveness to histamine in T. canis-infected guinea pigs and did not affect ileum contractility in non-infected animals. These results indicate that T. canis-infected guinea pigs develop increased intestinal responsiveness to histamine and that DSCG prevents alterations in smooth-muscle contractility.
Sá-Nunes
A
A
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Cafe s/no, Ribeirão Preto, 14040-903, São Paulo, Brazil.
Corrado
A P
AP
Baruffi
M D
MD
Faccioli
L H
LH
D000818:Animals; D004205:Cromolyn Sodium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D006633:Histamine Antagonists; D007082:Ileum / Q000187:drug effects / Q000503:physiopathology*; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects; D017166:Toxocara canis; D014120:Toxocariasis / Q000469:parasitology / Q000503:physiopathology*
D006633:Histamine Antagonists; D004205:Cromolyn Sodium