Establishment and characterization of a human uterine endometrial undifferentiated carcinoma cell line, TMG-L.
A new cell line of human uterine endometrial undifferentiated carcinoma, designated as TMG-L, was established from the metastatic lymph node of 56-year-old patient TMG-L cells have been cultured with Ham's F-12 medium supplemented with 10% FCS and grew as a loosely adherent monolayer with polygonal or spindle-shaped cells exhibiting poor cell-cell contact and piled up against each other, showing a tendency to grow as floating cells. The doubling time of this cell line was about 48 hours, and chromosomal analysis revealed aneuploidy at passage 25. The cells formed tumors in SCID mouse, the histology of which was similar to that of undifferentiated carcinoma component of primary tumor. TMG-L cells showed the loss of expression and membranous localization of either E-cadherin or alpha-catenin, implied corresponding loss of their adhesive function. And this dysfunction implicated the biological aggressive behavior of uterine endometrial undifferentiated carcinoma. This cell line appears to provide a useful system for studying uterine undifferentiated carcinoma in vivo and in vitro.
Hasegawa
Kiyoshi
K
Department of Obstetrics and Gynecology, Fujita Health University School of Medicine, Aichi, Japan. uda9294@fujita.hu.ac.jp
Suzuki
Machiko
M
Ishikawa
Kunimi
K
Yasue
Akira
A
Kato
Rina
R
Nakamura
Azumi
A
Kuroki
Jun
J
Udagawa
Yasuhiro
Y
D000782:Aneuploidy; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003584:Cytological Techniques; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D051178:alpha Catenin
D014408:Biomarkers, Tumor; C495273:CTNNA1 protein, human; D015820:Cadherins; C495272:Ctnna1 protein, mouse; D003598:Cytoskeletal Proteins; D016159:Tumor Suppressor Protein p53; D051178:alpha Catenin