title: Metabolic responses to dietary cholecalciferol and phosphorus in abalone Haliotis discus hannai ino.
abstract: Metabolic responses of cholecalciferol (VD(3)) and minerals (Ca, P and Mg) in abalone Haliotis discus hannai Ino to dietary VD(3) and phosphorus (P) were investigated. Based on a 2 x 2 factorial design, four casein-gelatin-based diets were formulated. The basal diet was supplemented with either 0 or 2000 IU VD(3)/kg diet and 0 or 10 g P/kg diet. The abalone was reared in P-free artificial seawater for 55 days. Results showed that dietary VD(3) was hydroxylated to 25-hydroxyvitamin D(3) [25(OH)D(3)] and 1 alpha,25-dihydroxyvitamin D(3) [1 alpha,25(OH)(2)D(3)] in abalone, and subsequently raised the serum levels of these two VD(3) metabolites. Dietary P deficiency elevated serum 1 alpha,25(OH)(2)D(3) level only when the dietary VD(3) supplementation was sufficient. The supplementations of either dietary VD(3) or P significantly increased the levels of P in serum, mantle and hepatopancreas, and only the addition of VD(3) significantly raised the concentrations of Ca in serum and mantle (P<0.05). Interaction between dietary VD(3) and P was only found significant on the concentrations of P and Mg in mantle (P<0.05). The concentrations of Ca, P and Mg in muscle were not significantly influenced by these dietary treatments. Hence, the metabolic responses in serum, muscle, mantle and hepatopancreas of abalone to dietary VD(3) and P were in different manners.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R. China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhang
authors.forename: Kangsen
authors.initials: K
authors.lastname: Mai
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Hongming
authors.initials: H
authors.lastname: Ma
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002136:Calcium, Dietary / Q000378:metabolism; D002762:Cholecalciferol / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000378:metabolism*; D011897:Random Allocation; D012908:Snails / Q000378:metabolism*
chemical_list: D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D002762:Cholecalciferol; D008274:Magnesium