title: [Production of recombinant fragments of the Clostridium tetani neurotoxin for the development of new immune-prophylaxis preparations against tetanus].
abstract: Tetanus belongs to dangerous infection diseases, whose effective prevention can be ensured by vaccines. The acting substance of tetanus vaccines, presently in use, is a partially purified and deprived-of-lethal-action Clostridium tetani neurotoxin. The construction of a subunit preparation on the basis of toxin fragments obtained through gene engineering could be a method aimed at promoting the quality of the used tetanus vaccines. With this goal in mind, we built, within the present case study, the expressing genetic constructions and obtained, in the pure form, an extensive tetanus-vaccine chain with its C-terminal (Hc) fragment, hydride peptides, containing the Hc-fragment and C-terminal fragment of toxin B C. difficile, as well as Hc-fragment and S3 collagen-binding domain of collagenase C. histolyticum. The thus obtained proteins can be used in testing their immunogenic and protective properties, while the conducted study could be a basis for further research of a new-generation vaccine against tetanus and other human infection diseases.
authors.affiliation: Gamaley's Research Institute for Epidemiology and Microbiology, Russia's Academy of Medical Sciences, Lomonosov's Moscow State University, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Varfolomeeva
authors.forename: O A
authors.initials: OA
authors.lastname: Makhotina
authors.forename: T I
authors.initials: TI
authors.lastname: Sergeeva
authors.forename: Iu F
authors.initials: IuF
authors.lastname: Belyĭ
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D016360:Clostridioides difficile / Q000235:genetics; D003013:Clostridium / Q000235:genetics; D017364:Collagenases / Q000235:genetics; D015195:Drug Design; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D013742:Tetanus / Q000517:prevention & control; D013744:Tetanus Toxin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic; D017364:Collagenases
vernacular_title: Poluchenie rekombinantnykh fragmentov neĭrotoksina Clostridium tetani dlia razrabotki novykh sredstv immunoprofilaktiki stolbniaka.