Buccal mucosal ulcer healing effect of rhEGF/Eudispert hv hydrogel.
We have studied the effect of rhEGF on the buccal mucosal ulcer healing. rhEGF was rapidly degraded upon incubation with the hamster buccal mucosal homogenates; The degradation of rhEGF was significantly inhibited by sodium lauryl sulfate (SLS). Eudispert hv hydrogel and Polycarbophil 974P hydrogel were prepared for rhEGF delivery and their mucoadhesiveness was measured by the Instron method. The mucoadhesive force of Eudispert hv was significantly greater than that of Polycarbophil 974P. Moreover, rhEGF in Eudispert hv hydrogel remained stable for about 2 months. To evaluate the ulcer healing effect of rhEGF, the buccal mucosal ulcer was induced in golden hamsters using acetic acid. At 24 h after administration of rhEGF/Eudispert hv hydrogel, the ulcerous area was decreased compared with rhEGF solution and, as a result, the curative ratio was 36.8 +/- 5.68%. By the addition of SLS (0.5%) to Eudispert hv hydrogel, the curative ratio increased 1.5 times. The mechanism of the action was probably due to a combination of protection of the drug against proteases present in mucosa and prolongation of the release of rhEGF from the formulation at the site of action.
Park
Jeong Sook
JS
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
Yoon
Joon Il
JI
Li
Hong
H
Moon
Dong Cheul
DC
Han
Kun
K
D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D006224:Cricetinae; D003692:Delayed-Action Preparations; D004355:Drug Stability; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D009061:Mouth Mucosa / Q000187:drug effects*; D019226:Oral Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
D003692:Delayed-Action Preparations; D020100:Hydrogels; D011109:Polymethacrylic Acids; D011994:Recombinant Proteins; C038300:methylmethacrylate-methacrylic acid copolymer; D004815:Epidermal Growth Factor; D019342:Acetic Acid