title: Molecular characterization of the full-length genome of the Japanese encephalitis viral strain K87P39.
abstract: We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain K87P39, isolated from a pool of circulating Culex tritaeniorhynchus mosquitoes in Korea. In comparison with 27 fully sequenced JEV genomes currently available, we found that the 10968-nucleotide RNA genome of K87P39 has a nine-nucleotide deletion in the 3' nontranslated variable region and that its single open reading frame has a total of eight amino acid substitutions. The K87P39 isolate is highly similar to other JEV isolates, and homology ranges from 97.9 to 89.0% at the nucleotide level, and 99.1 to 96.7% at the deduced amino acid level. Phylogenetic analyses using the full-length sequence of the 27 available JEV genomes showed that the K87P39 strain is most closely related to six Chinese SA14 derivatives and that it is distantly related to the Australian FU, Korean K94P05 and Japanese Ishikawa strains. In addition, we also found that phylogenetic relationships based on the full-length genome are highly similar to those based on the E gene, indicating that phylogenetic analysis of the E gene will be useful for studying the genetic relationships among JEV isolates. We therefore performed a more extensive E gene-based phylogenetic analysis on a selection of 70 JEV isolates available from GenBank, which represent a temporally and geographically wide variety of JEV strains.
authors.affiliation: Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, 48 Gaeshin-Dong, Heungduk-Ku, Cheongju, South Korea.
authors.forename: Sang-Im
authors.initials: SI
authors.lastname: Yun
authors.forename: Seok-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Choi
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Ju
authors.forename: Keun-Yong
authors.initials: KY
authors.lastname: Park
authors.forename: Hae-Wol
authors.initials: HW
authors.lastname: Cho
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D012367:RNA, Viral