Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene.
OBJECTIVE
To identify esophageal cancer related gene2 (ECRG2) associated proteins and their possible interactions with ECRG2 gene.


METHODS
In the yeast forward two-hybrid system, ECRG2 was fused with the DNA-binding domain (DBD) of Gal4 and human fetal liver cDNA library was fused with the transcriptional activation domain (AD) of Gal4. We performed a high-stringency scale procedure to screen ECRG2 against human fetal liver cDNA library and characterized positives by sequence analysis.


RESULTS
We found the following 9 putatively associated proteins. They were metallothionein2A, metallothionein1H, metallothionein1G, ferritin, erythrocyte membrane protein band4.2, mitochondrial ribosomal protein S12, hypothetical protein FLJ10101, and a novel gene whose cDNA was found to have no strong homology to any other previously characterized gene whose DDBJ/EMBL/GenBank accession number is AF422192 mapped to human chromosome 14q31.


CONCLUSIONS
MT, a potential interaction partner for ECRG2, might be involved in the regulation of cell proliferation and apoptosis, and in various physiological processes. Determination of a reliability score for each single protein-protein interaction, especially interaction of ECRG2 and MT, permits the assignment of ECRG2 and unannotated proteins to biological pathways. A further understanding of the association between ECRG2 and MT should facilitate the functions of ECRG2 gene.
Cui
Yong-Ping
YP
Department of Etiology and Carcinogenesis, Tumor Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
Wang
Jian-Bo
JB
Zhang
Xin-Yu
XY
Bi
Mei-Xia
MX
Guo
Li-Ping
LP
Lu
Shih-Hsin
SH
D005333:Fetus / Q000502:physiology; D015723:Gene Library; D006801:Humans; D008668:Metallothionein / Q000502:physiology*; D008969:Molecular Sequence Data; D053491:Proteinase Inhibitory Proteins, Secretory; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D053491:Proteinase Inhibitory Proteins, Secretory; C473799:SPINK7 protein, human; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins; D008668:Metallothionein