Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China.
A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome (SARS). SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market. All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates. The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known.
Guan
Y
Y
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China. yguan@hkucc.hku.hk
Zheng
B J
BJ
He
Y Q
YQ
Liu
X L
XL
Zhuang
Z X
ZX
Cheung
C L
CL
Luo
S W
SW
Li
P H
PH
Zhang
L J
LJ
Guan
Y J
YJ
Butt
K M
KM
Wong
K L
KL
Chan
K W
KW
Lim
W
W
Shortridge
K F
KF
Yuen
K Y
KY
Peiris
J S M
JS
Poon
L L M
LL
D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western; D002335:Carnivora / Q000821:virology*; D002681:China; D017934:Coronavirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000662:veterinary / Q000821:virology; D004197:Disease Reservoirs; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009666:Nose / Q000821:virology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C578557:spike glycoprotein, SARS-CoV