Detection and identification of Mycoplasma from bovine mastitis infections using a nested polymerase chain reaction.
A polymerase chain reaction (PCR) test was compared with culture for the detection and diagnosis of bovine Mycoplasma intramammary infection. The PCR test was applied to 24-hour Mycoplasma enrichment cultures of milk from cows with suspected mastitis and from bulk tank milk. In comparison to culture, the sensitivity and specificity of the PCR method were 96.2% and 99.1% for individual cow milk and 100% and 99.8% for the bulk tank milk, respectively. However, in discrepant cases where PCR was positive and culture was negative, the PCR test was correct; subsequent PCR tests and culturing of the individual cow's milk yielded positive results. The PCR test simultaneously detected and differentiated among 11 bovine Mycoplasma species.
Baird
S C
SC
Diagnostic Laboratories, College of Veterinary Medicine and Biological Sciences, Colorado State University, Ft. Collins, CO 80523, USA.
Carman
J
J
Dinsmore
R P
RP
Walker
R L
RL
Collins
J K
JK
D000818:Animals; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D009174:Mycoplasma / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013997:Time Factors
D017931:DNA Primers; D004269:DNA, Bacterial