title: Human embryonic stem cell lines derived from discarded embryos.
abstract: Human pluripotent embryonic stem (ES) cells have important potential in regenerative medicine and as models for human preimplantation development; however, debate continues over whether embryos should be destroyed to produce human ES cells. We have derived four ES cell lines on mouse embryonic fibroblast cells in medium supplemented with basic fibroblast growth factor, human recombinant leukemia inhibitory factor, and fetal bovine serum. The source of these cell lines was poor-quality embryos that in the course of routine clinical practice would have been discarded. After continuous proliferation in vitro for more than 12 months, these ES cell lines maintained their developmental potential to form trophoblast and somatic cells, including cardiac muscle and neuronal tissue.
authors.affiliation: BresaGen, Inc., Georgia Biobusiness Center, Athens 30605, USA. mitalipova@hotmail.com
authors.forename: Maisam
authors.initials: M
authors.lastname: Mitalipova
authors.forename: John
authors.initials: J
authors.lastname: Calhoun
authors.forename: Soojung
authors.initials: S
authors.lastname: Shin
authors.forename: David
authors.initials: D
authors.lastname: Wininger
authors.forename: Thomas
authors.initials: T
authors.lastname: Schulz
authors.forename: Scott
authors.initials: S
authors.lastname: Noggle
authors.forename: Alison
authors.initials: A
authors.lastname: Venable
authors.forename: Ian
authors.initials: I
authors.lastname: Lyons
authors.forename: Allan
authors.initials: A
authors.lastname: Robins
authors.forename: Steven
authors.initials: S
authors.lastname: Stice
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D019924:Embryo Disposition; D033041:Embryo Research; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D051379:Mice; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D015415:Biomarkers