title: Gram-positive bacteria specific properties of silybin derived from Silybum marianum.
abstract: Silybin has a potent antibacterial activity, more potent than silymarin II, against gram-positive bacteria without hemolytic activity, whereas it has no antimicrobial activity against gram-negative bacteria or fungi. The mode of action of silybin against the gram-positive bacterial cell was examined by investigating the change in plasma membrane dynamics of bacterial cells using 1,6-diphenyl-1,3,5-hextriene (DPH) as a membrane probe and by assessing the inhibition of macromolecular synthesis using radiolabeled incorporation assay. The results showed that silybin inhibited RNA and protein synthesis on gram-positive bacteria.
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Korea.
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
authors.forename: Hyung Keun
authors.initials: HK
authors.lastname: Kim
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Hye Gwang
authors.initials: HG
authors.lastname: Jeong
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D004912:Erythrocytes / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D020944:Silybum marianum / Q000737:chemistry*; D012329:RNA, Bacterial / Q000096:biosynthesis; D000077385:Silybin; D012838:Silymarin / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012838:Silymarin; D000077385:Silybin