title: Regulation of cytosolic pH and lactic acid release in mesangial cells overexpressing GLUT1.
abstract: BACKGROUND
  Anaerobic glycolysis leads to the formation of lactate and H+ and thus imposes a significant challenge on cytosolic acid/base regulation. Cytosolic acidification, on the other hand, is known to inhibit flux through glycolysis and lactate formation. To explore the interplay of cytosolic pH and glycolysis, rat mesangial cells transfected with the glucose transporter GLUT1 (GLUT1 cells) were compared with those transfected with beta-galactosidase (LacZ cells).
  
  
  METHODS
  In the presence of extracellular glucose, the glycolytic rate was one order of magnitude higher in GLUT1 cells than in LacZ cells. Cytosolic pH (pHi) was significantly higher in GLUT1 than LacZ cells, an effect abolished in the presence of Na+/H+ exchange inhibitor ethylisopropylamiloride (1 micromol/L).
  
  
  RESULTS
  Addition of 40 mmol/L lactate led to marked cytosolic acidification, which was in both cell types blunted by O-methyl-glucose (20 mmol/L) and completely abolished by 100 micromol/L phloretin and 1 mmol/L p-chloromercuribenzene-sulphonic acid (p-CMBS) and in LacZ cells only by glucose (20 mmol/L). The functional characterization points to the involvement of a lactic acid transporter from the monocarboxylate transporter (MCT) family, particularly MCT1. Reverse transcription-polymerase chain reaction (RT-PCR) indeed disclosed the expression of MCT1 and MCT2 in both GLUT1 and LacZ cells.
  
  
  CONCLUSIONS
  Overexpression of GLUT1 leads to cytosolic alkalinization of mesangial cells depending on functional Na+/H+ exchanger but not on Na+ independent H+ transport.
authors.affiliation: Department of Physiology, University of Tübingen, Tübingen, Germany.
authors.forename: Karl S
authors.initials: KS
authors.lastname: Lang
authors.forename: Matthias M
authors.initials: MM
authors.lastname: Mueller
authors.forename: Valerie
authors.initials: V
authors.lastname: Tanneur
authors.forename: Sabine
authors.initials: S
authors.lastname: Wallisch
authors.forename: Olga
authors.initials: O
authors.lastname: Fedorenko
authors.forename: Monica
authors.initials: M
authors.lastname: Palmada
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.forename: Stefan
authors.initials: S
authors.lastname: Bröer
authors.forename: Charles W
authors.initials: CW
authors.lastname: Heilig
authors.forename: Erwin
authors.initials: E
authors.lastname: Schleicher
authors.forename: Cora
authors.initials: C
authors.lastname: Weigert
mesh_terms: D000468:Alkalies / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002021:Buffers; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011522:Protons; D051381:Rats; D012964:Sodium / Q000172:deficiency / Q000502:physiology; D027981:Symporters / Q000378:metabolism; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D000468:Alkalies; D000643:Ammonium Chloride; D002021:Buffers; D051272:Glucose Transporter Type 1; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D009004:Monosaccharide Transport Proteins; D011522:Protons; D012964:Sodium; D027981:Symporters; D001616:beta-Galactosidase; D000099233:Monocarboxylate Transport Protein 1; C490427:SLC16A7 protein, human; C495677:SLC2A1 protein, human; C091633:Slc16a7 protein, rat; C495679:Slc2a1 protein, rat