Role of NF-kappa B in liver ischemia reperfusion injury of rats.
To determine the role of NF-kappa B in ischemia reperfusion (I/R) injury of the rat liver, rats underwent partial hepatic ischemia and reperfusion. The left and median lobes of the liver were subjected to ischemia for 90 min followed by reperfusion for defined times. NF-kappa B activity was analyzed by electrophoretic mobility shift assay (EMSA). Semiquantitative reverse-transcriptase polymerase chain reaction was used to analyze TNF-alpha and ICAM-1 mRNA levels. Results showed during liver I/R injury, NF-kappa B activation was induced in a time dependent manner. NF-kappa B was activated within 1 h and 2 h after the initiation of reperfusion and decreased after 4 h. Messenger RNA expression of TNF-alpha and ICAM-1 were increased after the reperfusion of 2 h. It was concluded that during hepatic I/R injury, NF-kappa B was activated and could bind to special sequence in the promoters of budget genes, which can up-regulate the expression of TNF-alpha and ICAM-1 mRNA to result in ischemia reperfusion injury of the rat liver.
Xu
Jun
J
Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Yang
Zhen
Z
Zeng
Jinhua
J
D000818:Animals; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1