title: Immunohistochemical endothelial localisation--a novel method of vessel delineation in tendon tissue.
abstract: The mechanism of tendon healing is still not fully understood. A dual source of nutrition for the tendon is important at times of injury either from synovial-type fluid bathing the tendon or its own blood supply. In addition, neovascularisation occurs at the site of injury from the time of the insult. The aim of this study was to develop a method of precise endothelial localisation in archived paraffin tendon sections, therefore facilitating the study of the healing of tendons within different species. The sections had to retain a high degree of cytoarchitecture and the stain be of enough contrast to allow quantitative assessment of tendon vascularity in different sites and different species. Endothelial staining was produced using an antibody to the endothelial cell surface marker CD-31 (Dako, Cambridge, UK). The signal was intensified using the Catalytic Signal Amplification kit (Dako). It resulted in a dark brown staining of the tendon endothelium, which was in sufficient contrast to allow automated image analysis.
authors.affiliation: The RAFT Institute, Mount Vernon Hospital, Northwood, HA6 2RN, Middlesex, UK. martin.jones@royalfree.nhs.org
authors.forename: Martin
authors.initials: M
authors.lastname: Jones
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Clayton
authors.forename: Carole
authors.initials: C
authors.lastname: Noel
authors.forename: Kaetan
authors.initials: K
authors.lastname: Ladhani
authors.forename: Addie
authors.initials: A
authors.lastname: Grobbelaar
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000293:injuries / Q000378:metabolism*; D005260:Female; D006736:Horses; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D009389:Neovascularization, Pathologic; D016612:Paraffin Embedding; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011817:Rabbits; D013708:Tendon Injuries / Q000378:metabolism / Q000473:pathology; D013710:Tendons / Q000098:blood supply* / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1