title: Effect of quercetin on hypoxic injury in freshly isolated rat proximal tubules.
abstract: The bioflavonoid quercetin, which has antioxidant properties, protects renal tubular epithelial cells from oxidant-induced injury by inhibiting lipid peroxidation. We examined the effect of quercetin on hypoxia-induced injury in freshly isolated rat renal proximal tubules. Hypoxia induced rapid loss of cellular ATP, followed by functional and structural alterations measured as a decrease in tubular potassium content and sequentially by an increase in lactate dehydrogenase release. Furthermore, hypoxia increased lipid peroxidation, measured as thiobarbituric acid-reactive substances. Quercetin significantly inhibited hypoxia-induced functional and structural tubular injury in addition to lipid peroxidation but did not alter hypoxia-induced ATP depletion. These results demonstrate the potency of the bioflavonoid quercetin in protecting proximal tubules from hypoxic injury, which is independent of tubular energy metabolism and may be related to the inhibition of lipid peroxidation.
authors.affiliation: Klinik für Nieren- und Hochdrukkrankheiten, Universitätsklinikum, 45122, Essen, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Pietruck
authors.forename: Martin K
authors.initials: MK
authors.lastname: Kuhlmann
authors.forename: Bettina
authors.initials: B
authors.lastname: Lange
authors.forename: Thorsten
authors.initials: T
authors.lastname: Feldkamp
authors.forename: Stefan
authors.initials: S
authors.lastname: Herget-Rosenthal
authors.forename: Ursula
authors.initials: U
authors.lastname: Rauen
authors.forename: Gunther
authors.initials: G
authors.lastname: Burkhardt
authors.forename: Hans
authors.initials: H
authors.lastname: Kohler
authors.forename: Thomas
authors.initials: T
authors.lastname: Philipp
authors.forename: Andreas
authors.initials: A
authors.lastname: Kribben
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000860:Hypoxia / Q000188:drug therapy*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D011188:Potassium