title: Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity.
abstract: A novel L-amino acid oxidase, named TSV-LAO, has been purified and cloned from the snake Trimeresurus stejnegeri. Fifty percentage cytotoxic concentrations (CC50) of TSV-LAO on C8166 cells were 24 and 390 nM in the absence or presence of catalase (400 nM), respectively. However, at concentrations that showed little effect on cell viability, TSV-LAO displayed dose dependent inhibition on HIV-1 infection and replication. The antiviral selectivity indexes (CC50/EC50) were 16 and 6, respectively, corresponding to the measurements of syncytium formation and HIV-1 p24 antigen expression. Interestingly, the presence of catalase resulted in an increase of its antiviral selectivity to 52 and 38. Under the same conditions, no anti-HIV-1 activity was observed by exogenous addition of H2O2. The complete amino acid sequence of TSV-LAO, as deduced from its cDNA, exhibits a high degree of sequence identity with other snake venom LAOs.
authors.affiliation: Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Lee
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D015497:HIV-1 / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D050830:L-Amino Acid Oxidase; D008969:Molecular Sequence Data; D014757:Viper Venoms / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D019380:Anti-HIV Agents; D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D014757:Viper Venoms; D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; C478161:TSV-LAO protein, Trimeresurus stejnegeri