[8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate inhibited the reduction of cerebral blood flow evoked by 5-HT and KCl in rats].
OBJECTIVE
To investigate the impact of 8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate (TMB-8) on the change of cerebral blood flow(CBF) induced by 5-HT or KCl in rats.


METHODS
The CBF in rats was measured by a Laser-Doppler flowmeter. The cranial window field was superfused with artificial cerebral spinal fluid containing TMB-8, 5-HT or KCl.


RESULTS
12.5, 25 and 50 mumol.L-1 TMB-8 showed no significant effect on rest CBF in rats. 12.5, 25 and 50 mumol.L-1 TMB-8 apparently inhibited the decline of CBF evoked by 1 or 2 mumol.L-1 5-HT. When persistent reduction of CBF were evoked by 1 mumol.L-1 5-HT, TMB-8 markedly increased the CBF in a concentration-dependent manner. The reduction of CBF induced by 20 or 40 mmol.L-1 KCl was also suppressed by 12.5, 25 and 50 mumol.L-1 TMB-8. While persistent reduction of CBF was evoked by 20 mmol.L-1 KCl. TMB-8 markedly increased the CBF in a concentration-dependent manner.


CONCLUSIONS
These results indicate that TMB-8 is effective in preventing and treating the reduction of CBF induced by 5-HT or KCl, and improved the supply of blood in rat brain during ischemia.
Wang
Bin
B
Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China. binwang@njmu.edu.cn
Zhang
Ai-xia
AX
Zou
Ying
Y
Wang
Juan
J
Xiao
Ji-gao
JG
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004347:Drug Interactions; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011189:Potassium Chloride / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D012702:Serotonin Antagonists / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D012702:Serotonin Antagonists; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D011189:Potassium Chloride