title: Limited reliability of E-cadherin as a specific marker for in vitro-generated Langerhans cells.
abstract: Langerhans cells (LC) are a unique population of dendritic cells (DC) found in the epidermis where they can be identified by the expression of CD1a, E-cadherin and cytoplasmic Birbeck granules (BG) as their hallmark. Over the past years many techniques have been described to generate LC in vitro from either monocytes or CD34(+) hematopoietic cell progenitors. Antibodies against Lag and Langerin (two epitopes associated with BG) and E-cadherin (a Ca(2+)-dependent homophilic adhesion molecule) have been used to detect in vitro-generated LC. In this study we investigated whether the expression of E-cadherin on in vitro-generated CD1a(+) from either CD34(+) cells or monocytes is able to discriminate LC from other DC. Our results demonstrate that E-cadherin alone is not a reliable marker to specifically identify in vitro-generated LC.
authors.affiliation: Department of Dermatology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
authors.forename: J-P
authors.initials: JP
authors.lastname: Allam
authors.forename: E
authors.initials: E
authors.lastname: Geiger
authors.forename: S
authors.initials: S
authors.lastname: Kraft
authors.forename: T
authors.initials: T
authors.lastname: Bieber
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers; D015820:Cadherins / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods / Q000592:standards*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D007801:Langerhans Cells / Q000166:cytology* / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D000093322:Lymphocyte Activation Gene 3 Protein
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; D015415:Biomarkers; C121916:CD207 protein, human; D015820:Cadherins; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D000093322:Lymphocyte Activation Gene 3 Protein; C000720326:Lag3 protein, human