title: Aberrations of p16INK4A, p14ARF and p15INK4B genes in pediatric solid tumors.
abstract: Expression of the p16INK4A (p16), p15INK4B (p15), and p14ARF genes, located at 9p21, was examined in pediatric neuroblastoma (NB), Ewing's sarcoma (ES), and rhabdomyosarcoma (RMS). p16 expression was absent in 4 of 5 ESs, and 2 of these 4 cases died. p16 expression was reduced or absent in 10 of 12 RMSs, and 4 of these 10 cases died. These results suggested the possibility that p16 expression was associated with the progression of ES and RMS. There has been no previous report on p14ARF in NB. Our investigation might indicate that abnormal expression of the p16 and p14ARF was associated with a poor prognosis in NB, although in some cases of NB normal p16 and abnormal p14ARF expression was seen. These findings suggest an important role of p14ARF gene in the tumorigenesis of NB. The different incidence of expression of the p16, p15, and p14ARF genes in these 3 tumor types may reflect differences of the molecular process through which the 3 tumors develop. Our results suggest that abnormal expression of the p16 and/or p14ARF may be associated with a poor prognosis in these 3 tumors.
authors.affiliation: Department of Pediatric Surgery, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Obana
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yang
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Piao
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Taki
authors.forename: Kohei
authors.initials: K
authors.lastname: Hashizume
authors.forename: Ryoji
authors.initials: R
authors.lastname: Hanada
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yukichi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Toyoda
authors.forename: Junko
authors.initials: J
authors.lastname: Takita
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tsuchida
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D017353:Gene Deletion; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics / Q000401:mortality; D011379:Prognosis; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000401:mortality; D012512:Sarcoma, Ewing / Q000235:genetics / Q000401:mortality; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D025803:Tumor Suppressor Protein p14ARF; D025521:Tumor Suppressor Proteins; D012313:RNA; D004247:DNA