title: Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury.
abstract: To characterize the features of JAK/STAT signaling in Drosophila immune response, we have identified totA as a gene that is regulated by the JAK/STAT pathway in response to septic injury. We show that septic injury triggers the hemocyte-specific expression of upd3, a gene encoding a novel Upd-like cytokine that is necessary for the JAK/STAT-dependent activation of totA in the Drosophila counterpart of the mammalian liver, the fat body. In addition, we demonstrate that totA activation also requires the NF-KB-like Relish pathway, indicating that fat body cells integrate the activity of NF-KB and JAK/STAT signaling pathways upon immune response. This study reveals that, in addition to the pattern recognition receptor-mediated NF-KB-dependent immune response, Drosophila undergoes a complex systemic response that is mediated by the production of cytokines in blood cells, a process that is similar to the acute phase response in mammals.
authors.affiliation: Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA. agaisse@rascal.med.harvard.edu
authors.forename: Hervé
authors.initials: H
authors.lastname: Agaisse
authors.forename: Ulla Maja
authors.initials: UM
authors.lastname: Petersen
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
authors.forename: Bernard
authors.initials: B
authors.lastname: Mathey-Prevot
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D001952:Bridged-Ring Compounds / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007239:Infections; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D019076:Transgenes
chemical_list: D001952:Bridged-Ring Compounds; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; C101626:Rel protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; C444499:dome protein, Drosophila; D011505:Protein-Tyrosine Kinases; C010349:tetramethylenedisulfotetramine