A new micellar electrokinetic capillary chromatography method for separation of the components of the aminoglycoside antibiotics.
Aminoglycoside antibiotics are always a mixture of structurally related amino sugars, which do not have a chromophore or fluorophore. The aim of the study was to find one method for evaluation of the components and impurities of the antibiotics. Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for all antibiotics. The components of gentamicin (GM), sisomicin, netilmicin, kanamycin, amikacin, and tobramycin were tried to separate by means of micellar electrokinetic chromatography. The background electrolyte was composed of sodium tetraborate (100 mM, pH 10.0), sodium deoxycholate (20 mM), and beta-cyclodextrin (15 mM). This method is valid for evaluation of GM, kanamycin, and tobramycin. It has to be improved for amikacin and netilmicin. In addition, 46 bulk samples of GM of different manufacturer or pharmaceutical companies were investigated. Many samples were found to contain many minor products and different amounts. Beside different patterns of the main compounds GM C1, GM C1a, GM C2a, and GM C2, many lots were found consisting of a substantial number of minor products. The appearance of a high number of minor products is always associated with the existence of sisomicin, which is not found in "pure" samples. However, almost all samples met the requirements of the European Pharmacopoeia (EP) and United States Pharmacopoeia (USP).
Wienen
Frank
F
Institute of Pharmacy and Food Chemistry, University of Würzburg,Würzburg, Germany.
Holzgrabe
Ulrike
U
D000583:Amikacin / Q000032:analysis / Q000737:chemistry; D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003840:Deoxycholic Acid / Q000737:chemistry; D005839:Gentamicins / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007612:Kanamycin / Q000032:analysis / Q000737:chemistry; D009428:Netilmicin / Q000032:analysis / Q000737:chemistry; D012853:Sisomicin / Q000032:analysis / Q000737:chemistry; D013696:Temperature; D014031:Tobramycin / Q000032:analysis / Q000737:chemistry
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D003840:Deoxycholic Acid; D009428:Netilmicin; D007612:Kanamycin; D000583:Amikacin; D014031:Tobramycin; D012853:Sisomicin