Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA.
We established an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of bovine macrophage colony-stimulating factor (M-CSF) and used it to measure the serum M-CSF levels in bovine fetuses and calves. The average serum M-CSF level was 2.7+/-1.5 ng/ml in 39 calves under 100 days old, and 1.8+/-0.8 ng/ml in 15 cattle between 101 and 418 days old. Fetal sera samples (n = 6) prepared from cattle between 150 and 280 days of gestational age had a higher average level of M-CSF (8.8+/-1.4 ng/ml). Alteration in serum M-CSF levels in each individual calf was also measured. The serum levels of M-CSF in calves at 0-1 day after birth ranged from 0.52 to 7.3 ng/ml. During the period 113-125 days after birth, serum levels were around 1.4+/-0.39 ng/ml. Although serum M-CSF levels generally decreased as the age of calves advanced, differences among individuals, especially among newborn calves, were observed.
Yoshihara
K
K
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. kazukazu@affrc.go.jp
Oshima
K
K
Muneta
Y
Y
Kikuma
R
R
Yayota
C
C
Hirai
T
T
Satoh
N
N
Matsuura
S
S
Kikyo
Y
Y
Satoh
M
M
Kubota
C
C
Inumaru
S
S
Yokomizo
Y
Y
Mori
Y
Y
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002417:Cattle / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005333:Fetus; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D011247:Pregnancy; D012680:Sensitivity and Specificity
D016173:Macrophage Colony-Stimulating Factor