Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1.
Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae) has been shown to possess anti-microbial, anti-tumoral, and anti-inflammatory properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. These results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage.
Moon
Mi Kyoung
MK
Medicinal Resources Research Center, School of Medicine, Wonkwang University, Iksan,, Chonbuk 570-749 South Korea.
Choi
Byung-Min
BM
Oh
Gi-Su
GS
Pae
Hyun-Ock
HO
Kim
Jong-Duck
JD
Oh
Hyuncheol
H
Oh
Cheon-Sik
CS
Kim
Dae-Han
DH
Rho
Young-Deuk
YD
Shin
Min-Kyo
MK
Lee
Ho-Sub
HS
Chung
Hun-Taeg
HT
D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D015153:Blotting, Western; D002248:Carbon Monoxide / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004232:Diuretics / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects
D004232:Diuretics; D005960:Glucosides; D008565:Membrane Proteins; D018696:Neuroprotective Agents; D016877:Oxidants; C006928:catalposide; D002248:Carbon Monoxide; D006861:Hydrogen Peroxide; D007501:Iron; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D001663:Bilirubin