Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers.
Three bovine amphistomes, Calicophoron calicophorum, Orthocoelium streptocoelium and Homalogaster paloniae, are common in Japan. This study was carried out to describe ITS2 sequences in the 3 species, and to evaluate a PCR-RFLP technique based on ITS2 sequence for the species identification in single eggs of the parasites. The ITS2 sequences of the three species contained 19 variable sites including one gap. The sequence difference between species was 4.2-5.3%. The three species of amphistomes were identified based on the difference in the restriction sites of Acc II on the ITS2 sequence.
Itagaki
Tadashi
T
Department of Disease Control, Iwate University, Morioka, Japan.
Tsumagari
Nobuyuki
N
Tsutsumi
Ken-ichi
K
Chinone
Shiro
S
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000469:parasitology*; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology*; D004247:DNA / Q000235:genetics*; D018509:DNA, Helminth; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014200:Trematoda / Q000145:classification / Q000235:genetics*; D014201:Trematode Infections / Q000175:diagnosis / Q000662:veterinary*
D018509:DNA, Helminth; D005819:Genetic Markers; D004247:DNA