title: Augmentation of ethanol-induced cell damage and activation of nuclear factor-kappa B by annexin IV in cultured cells.
abstract: BACKGROUND
  We previously reported that the amount of annexin IV expression was increased in culture cells by exposure to ethanol. Here, to investigate the physiologic role of annexin IV, we analyzed ethanol-induced cytotoxicity and nuclear factor (NF)-kappa B activity by using annexin IV-overexpressed cells.
  
  
  METHODS
  Annexin IV overexpression was performed by transfection of expression vector, in which annexin IV complementary DNA was ligated, to culture cells (rat glioma C6 cells and human neuroblastoma SH-SY5Y cells). Ethanol-induced cytotoxicity was assayed by measuring the mitochondrial enzyme (dehydrogenase) activity or trypan blue exclusion. NF-kappa B activity was measured by electrophoretic mobility shift assay with a kappa B-oligonucleotide probe.
  
  
  RESULTS
  Ethanol-induced cytotoxicity was increased by overexpression of annexin IV in both C6 cells and SH-SY5Y cells. Annexin IV overexpression augmented ethanol-induced NF-kappa B activation.
  
  
  CONCLUSIONS
  Ethanol-induced increase in annexin IV expression might amplify ethanol-induced cytotoxicity via NF-kappa B activation.
authors.affiliation: Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo, Japan. sohma@sapmed.ac.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sohma
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ohkawa
authors.forename: Rie
authors.initials: R
authors.lastname: Sakai
authors.forename: Eri
authors.initials: E
authors.lastname: Hashimoto
authors.forename: Wataru
authors.initials: W
authors.lastname: Ukai
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D017314:Annexin A4 / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects*; D024202:Electrophoretic Mobility Shift Assay; D000431:Ethanol / Q000633:toxicity*; D005910:Glioma; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D009447:Neuroblastoma; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects*
chemical_list: D017314:Annexin A4; D016328:NF-kappa B; D000431:Ethanol