title: Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs.
abstract: The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.
authors.affiliation: USDA/ARS, Meat Animal Research Center, Clay Center, NE 68933, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kwang
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: F A
authors.initials: FA
authors.lastname: Osorio
authors.forename: S
authors.initials: S
authors.lastname: Christian
authors.forename: J G
authors.initials: JG
authors.lastname: Wheeler
authors.forename: K M
authors.initials: KM
authors.lastname: Lager
authors.forename: S
authors.initials: S
authors.lastname: Low
authors.forename: L
authors.initials: L
authors.lastname: Chang
authors.forename: A R
authors.initials: AR
authors.lastname: Doster
authors.forename: A
authors.initials: A
authors.lastname: White
authors.forename: C C
authors.initials: CC
authors.lastname: Wu
mesh_terms: D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D015588:Observer Variation; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000472:pathogenicity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine