title: Medial preoptic area adrenergic receptors modulate glycemia and insulinemia in freely moving rats.
abstract: In order to investigate the role of medial preoptic area (MPOA) adrenoceptors in regulation of plasma glucose and insulin secretion, we injected 40 nmol of noradrenaline, clonidine or isoproterenol into the MPOA of freely moving Wistar rats. The animals were fitted with chronic jugular catheters for blood sampling and unilateral intracerebral cannulae placed into MPOA. The results showed that noradrenaline injection into MPOA produced a rapid increase in plasma glucose levels and insulin secretion, reaching a peak at 15 min post stimulus (25% over basal, P<0.01) for plasma glucose and at 30 min for insulin secretion (94% over basal, P<0.05). Injection of the alpha2-adrenergic agonist clonidine into MPOA produced a faster, more intense and longer-lasting hyperglycemic response (69% over basal, P<0.01). In contrast to the noradrenaline effect on insulin secretion, clonidine markedly decreased plasma insulin levels, reaching a maximal suppression at 10 min (72% below basal, P<0.01). On the other hand, the beta-adrenergic agonist isoproterenol only produced a small, transient increase in plasma glucose levels. When rats were pre-treated with guanethidine (10 mg/100 g, i.p.), despite reduced baseline of plasma glucose (35% smaller then control group, P<0.01) and increased plasma insulin baseline (300% higher then control group, P<0.01), they still showed a hyperglycemic response to noradrenaline injection into MPOA. We conclude that the activation of preoptic alpha2-adrenoceptors induced hyperglycemia and inhibit insulin secretion, probably by activation of the sympathoadrenal system that cannot be blocked by prior administration of guanethidine.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biological Science, Federal University of Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Rodrigo Bastos
authors.initials: RB
authors.lastname: Fóscolo
authors.forename: Mauricio Gustavo Bravim
authors.initials: MG
authors.lastname: de Castro
authors.forename: Umeko
authors.initials: U
authors.lastname: Marubayashi
authors.forename: Adelina Martha
authors.initials: AM
authors.lastname: dos Reis
authors.forename: Cândido Celso
authors.initials: CC
authors.lastname: Coimbra
mesh_terms: D018663:Adrenergic Agents / Q000494:pharmacology; D000316:Adrenergic alpha-Agonists / Q000008:administration & dosage / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D003000:Clonidine / Q000008:administration & dosage / Q000494:pharmacology; D006145:Guanethidine / Q000494:pharmacology; D007276:Injections, Intraventricular; D007328:Insulin / Q000097:blood*; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D009068:Movement; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D011301:Preoptic Area / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology*; D013997:Time Factors
chemical_list: D018663:Adrenergic Agents; D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D001786:Blood Glucose; D007328:Insulin; D011941:Receptors, Adrenergic; D007545:Isoproterenol; D003000:Clonidine; D009638:Norepinephrine; D006145:Guanethidine