title: Molecular evolution and diversification of snake toxin genes, revealed by analysis of intron sequences.
abstract: The genes encoding erabutoxin (short chain neurotoxin) isoforms (Ea, Eb, and Ec), LsIII (long chain neurotoxin) and a novel long chain neurotoxin pseudogene were cloned from a Laticauda semifasciata genomic library. Short and long chain neurotoxin genes were also cloned from the genome of Laticauda laticaudata, a closely related species of L. semifasciata, by PCR. A putative matrix attached region (MAR) sequence was found in the intron I of the LsIII gene. Comparative analysis of 11 structurally relevant snake toxin genes (three-finger-structure toxins) revealed the molecular evolution of these toxins. Three-finger-structure toxin genes diverged from a common ancestor through two types of evolutionary pathways (long and short types), early in the course of evolution. At a later stage of evolution in each gene, the accumulation of mutations in the exons, especially exon II, by accelerated evolution may have caused the increased diversification in their functions. It was also revealed that the putative MAR sequence found in the LsIII gene was integrated into the gene after the species-level divergence.
authors.affiliation: Faculty of Science and Technology, Sophia University, 7-1 Chiyoda-ku, 102-8554 Tokyo, Japan.
authors.forename: T J
authors.initials: TJ
authors.lastname: Fujimi
authors.forename: T
authors.initials: T
authors.lastname: Nakajyo
authors.forename: E
authors.initials: E
authors.lastname: Nishimura
authors.forename: E
authors.initials: E
authors.lastname: Ogura
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: T
authors.initials: T
authors.lastname: Tamiya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017815:Elapidae / Q000235:genetics*; D004870:Erabutoxins / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009498:Neurotoxins; D004870:Erabutoxins; D004247:DNA