Absence of inhibition of cutaneous wound healing in mice by oral doxycycline.
Temporally controlled expression of genes in transgenic mice has advanced our understanding of many physiological processes. One of the more common modes of acutely altering gene levels involves the doxycycline-regulated "tet-on/tet-off" systems. There has been concern that the administration of doxycycline in itself might compromise many aspects of wound repair. Here we report that oral ad libitum administration of doxycycline (2 mg/ml in drinking water) to mice does not alter dermal or epidermal wound healing. The healing of both full- and partial-thickness skin wounds proceeded similarly regardless of doxycycline administration; in fact, collagen organization and tensile strength development appeared to accelerate in the presence of doxycycline. These data suggest that wound healing studies incorporating transgene expression can utilize tet-on/tet-off regulation of gene expression without interference from doxycycline.
Hebda
Patricia A
PA
Department of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA. hebda@pitt.edu
Whaley
Diana
D
Kim
Hyung-Gyoon
HG
Wells
Alan
A
D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D004318:Doxycycline / Q000008:administration & dosage*; D005109:Extracellular Matrix / Q000187:drug effects; D015603:Keratinocytes / Q000187:drug effects*; D051379:Mice; D008822:Mice, Transgenic; D012867:Skin / Q000187:drug effects / Q000293:injuries; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D004318:Doxycycline