Sustained intracellular Ca2+ elevation induced by a brief BDNF application in rat visual cortex neurons.
A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats. BDNF increased intracellular Ca2+ through the PLC-gamma phosphorylation after the TrkB receptor tyrosine kinase activation. Either K252a or U73122 suppressed intracellular Ca2+ in the absence of BDNF. We suggest that sustained activation of Trk B receptor tyrosine kinase and PLC-gamma occurs after a brief BDNF application and contributes to the short-term maintenance (< 30 min) of the sustained intracellular Ca2+ elevation.
Mizoguchi
Yoshito
Y
Cellular and Systems Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Nabekura
Junichi
J
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002227:Carbazoles / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D066298:In Vitro Techniques; D026121:Indole Alkaloids; D051966:Phospholipase C gamma; D010766:Phosphorylation; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism*; D011760:Pyrrolidinones / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism; D014793:Visual Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D002227:Carbazoles; D004791:Enzyme Inhibitors; D004963:Estrenes; D005456:Fluorescent Dyes; D026121:Indole Alkaloids; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C049985:staurosporine aglycone; D020813:Receptor, trkB; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D002118:Calcium; D016257:Fura-2