Both aging and chronic fluoxetine increase S100B content in the mouse hippocampus.
S100B is a cytokine with neurotrophic and neurite-extending activity that has been implicated in the mechanism of action of anti-depressants and in the pathobiology of aging associated disorders such as Alzheimer's disease. The antidepressant fluoxetine increases hippocampal S100B content in young adult rats. In humans, brain levels of S100B mRNA and protein increase with advancing age. We assayed hippocampal S100B protein content in young (2 month) and old (24 month) mice, and in old mice treated for 2 weeks with fluoxetine. Using quantitative Western immunoblotting and an immunoassay kit we found higher S100B content in the hippocampus of old mice. Fluoxetine treatment of old mice further increased hippocampal S100B, suggesting that aging does not interfere with fluoxetine's action on hippocampal S100B.
Akhisaroglu
Mustafa
M
The Psychiatric Institute, Department of Psychiatry, University of Illinois at Chicago, 1601 West Taylor Street, MC912, Chicago, IL 60612, USA.
Manev
Radmila
R
Akhisaroglu
Ezgi
E
Uz
Tolga
T
Manev
Hari
H
D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D005473:Fluoxetine / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D007118:Immunoassay; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000378:metabolism*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*; D013268:Stimulation, Chemical
D005473:Fluoxetine; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D017367:Selective Serotonin Reuptake Inhibitors; C579160:S100B protein, human; C579159:S100b protein, mouse; C579158:S100b protein, rat