Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis.
We have identified a phospholipase D gene (pld) fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted.
Leem
Young Eun
YE
Microbial Physiology Lab, Division of Life Sciences, Kangwon National University, Chunchon 200-701, South Korea.
Ross
Ian K
IK
Choi
Hyoung Tae
HT
D000363:Agaricales / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D007621:Karyotyping; D042845:Laccase / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010739:Phospholipase D / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D004271:DNA, Fungal; D042845:Laccase; D004262:DNA Restriction Enzymes; D010739:Phospholipase D