title: Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers.
abstract: The isolation and phylogenetic characterization of acidophilic moderate thermophilic bacteria from different locations of uranium mines and a uranium processing mill in Pakistan is reported. The dominant culturable bacteria found were related to Sulfobacillus thermosulfidooxidans in all the samples analyzed. Different strains displayed different levels of identity (95-97%) to 16S rDNA of known strains of this species, indicating group heterogeneity. Genomic DNA from five isolates was subjected to amplification using integron-specific primers HS286 and HS287. Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies.
authors.affiliation: National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Ghauri
authors.forename: Ahmad M
authors.initials: AM
authors.lastname: Khalid
authors.forename: Susan
authors.initials: S
authors.lastname: Grant
authors.forename: Shaun
authors.initials: S
authors.lastname: Heaphy
authors.forename: William D
authors.initials: WD
authors.lastname: Grant
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D032023:Integrons; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S