title: Effects of transcorneal iridal photocoagulation on the canine corneal endothelium using a diode laser.
abstract: OBJECTIVE
  To investigate the potential damage to the canine corneal endothelium following transcorneal iridal laser photocoagulation using a semiconductor diode laser.
  
  
  METHODS
  Sixteen young mongrel dogs.
  
  
  METHODS
  Baseline corneal endothelial cell counts and corneal thickness were measured in the central and temporal quadrants using a noncontact specular microscope under general anesthesia. Transcorneal iridal photocoagulation was applied using a semiconductor diode laser in a continuous mode with the use of an operating microscope. Fifteen dogs were treated, and the sixteenth dog served as a control. Fifteen different treatment combinations were randomly assigned to the 30 eyes; the fellow eye was treated differently. Three treatment factors were investigated: (1) laser energy intensity, (2) target tissue to endothelial distance, and (3) laser application duration. After 3 weeks the dogs were euthanized, specular microscopy was repeated, and the cornea was examined by scanning electron microscopy.
  
  
  RESULTS
  Dyscoria and focal iris darkening were noted in all eyes immediately following laser treatment. Focal corneal edema (n = 2) and an incipient anterior capsular cataract (n = 1) were also noted. Baseline mean corneal endothelial cell densities were 2530 cells/mm2 centrally and 2607 cells/mm2 temporally. Postlaser corneal endothelial cell densities were 2499 cells/mm2 centrally and 2523 cells/mm2 temporally. Mean prelaser corneal thickness measurements were 0.555 mm centrally and 0.549 mm temporally. Postlaser corneal thickness measurements were 0.580 mm centrally and 0.554 mm temporally. Statistical analyzes revealed no significant changes in endothelial cell densities (P > 0.05) or corneal thickness (P > 0.05) induced by any treatment combination. Aside from tissue handling and processing artifacts, scanning electron microscopy revealed no endothelial cell damage.
  
  
  CONCLUSIONS
  Our study demonstrated by specular and scanning electron microscopy that diode laser iridal photocoagulation had no significant effect on the canine corneal endothelium within the parameters described. However, one must take into consideration the young age of the dogs and the potential for corneal endothelial cell regeneration in young dogs, and the relatively short period of postoperative study.
authors.affiliation: Department of Small Animal Medicine, College of Veterinary Medicine, Department of Statistics, University of Georgia, 501 DW Brooks Dr, Athens, GA 30602, USA. mjchandler@yahoo.com
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Chandler
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Moore
authors.forename: Ursula M
authors.initials: UM
authors.lastname: Dietrich
authors.forename: Charles L
authors.initials: CL
authors.lastname: Martin
authors.forename: Anand
authors.initials: A
authors.lastname: Vidyashankar
authors.forename: Guorong
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D003315:Cornea / Q000473:pathology*; D004285:Dogs; D004728:Endothelium, Corneal / Q000528:radiation effects*; D005260:Female; D053685:Laser Therapy / Q000009:adverse effects / Q000662:veterinary; D008028:Light Coagulation / Q000009:adverse effects / Q000662:veterinary*; D008297:Male