title: Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs.
abstract: N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated.
authors.affiliation: Department of Biochemistry, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba 305-8602, Japan. akimotoc@nias.affrc.go.jp
authors.forename: Chiharu
authors.initials: C
authors.lastname: Akimoto-Tomiyama
authors.forename: Katsumi
authors.initials: K
authors.lastname: Sakata
authors.forename: Junshi
authors.initials: J
authors.lastname: Yazaki
authors.forename: Keiko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Fumiko
authors.initials: F
authors.lastname: Fujii
authors.forename: Kanako
authors.initials: K
authors.lastname: Shimbo
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takuji
authors.initials: T
authors.lastname: Sasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Kishimoto
authors.forename: Shoshi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.forename: Eiichi
authors.initials: E
authors.lastname: Minami
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009844:Oligosaccharides / Q000494:pharmacology*; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D009844:Oligosaccharides; D011494:Protein Kinases; D018749:RNA, Plant; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C082723:N-acetylchitooctaose