[Determination of deoxyribonucleic acid with alkali blue 6B by resonance light-scattering method].
Characteristics of the interaction between alkali blue 6B with deoxyribonucleic acid (DNA) was studied by resonance light-scattering (RLS) method in the pH 2.90 medium. The effects of interference substances and the optimization of procedure were investigated, and a new method based on RLS was established. The method can be used to determine the DNA at nanogram level. The linear range of the DNA is 10-100.4 micrograms.L-1 with a correlation coefficient of 0.9913. The detection limit is 4.2 micrograms-L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory results.
Long
Yun-fei
YF
Institute of Chemistry, Xiangtan University, Department of Chemistry, Xiangtan Normal University, Xiangtan, China.
Chen
Xiao-ming
XM
Wu
Qun-li
QL
Yang
Wei-jun
WJ
D000814:Aniline Compounds / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D036103:Nanotechnology; D012542:Scattering, Radiation
D000814:Aniline Compounds; D001557:Benzenesulfonates; C111519:alkali blue 6B; D004247:DNA