title: Enhancement of antigen-presenting capacity and antitumor immunity of dendritic cells pulsed with autologous tumor-derived RNA in mice.
abstract: Dendritic cells (DCs) are antigen-presenting cells that play an important role in antitumor immunity. Several studies have reported that DCs pulsed with RNA from tumor cells have the ability to suppress tumors, but the details regarding the function and the immune-mechanism of DCs transfected with RNA remain to be elucidated. In this study, we investigated the transfection efficiency of RNA into DCs, and the functional modification and the antitumor efficacy of DCs pulsed with tumor-derived RNA. After the transfection of tumor-derived RNA into DCs cultured from the bone marrow of mice, pulsed DCs exhibited a high expression of both MHC antigens and CD86 on the cell surface as well as cultured DCs, and had a stronger ability both to present antigen on the MHC antigens and to stimulate T cells compared with DCs without transfection. DCs could sufficiently translate luciferase encoding RNA into luciferase proteins, and luciferase protein was expressed up to 12 hours in pulsed DCs. The DCs pulsed with tumor-derived RNA could elite a potent induction of cytotoxic T lymphocytes against autologous tumors, but not lysis against syngeneic normal cells. RNA-pulsed DCs exhibited a significant antitumor immunity in animal model. In conclusion, DCs could sufficiently uptake exogenous tumor-derived RNA, and consequently grow to be an intermediate maturate type, and induce potent T-cell stimulation and fully cause an antitumor effect in vivo. Therapy with DCs pulsed with tumor-derived RNA is sufficiently effective and safe, and thus it is considered to be clinically useful for tumor-immunotherapy.
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashiku, Fukuoka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Minami
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Miiru
authors.initials: M
authors.lastname: Izumi
authors.forename: Taishi
authors.initials: T
authors.lastname: Harada
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hara
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017951:Antigen Presentation; D001854:Bone Marrow Cells / Q000166:cytology; D018827:Carcinoma, Lewis Lung; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D004705:Endocytosis; D005260:Female; D017930:Genes, Reporter; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation; D008546:Melanoma, Experimental; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000628:therapy*; D012334:RNA, Neoplasm; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
chemical_list: D012334:RNA, Neoplasm; D012321:DNA-Directed RNA Polymerases