Capillary high-performance liquid chromatographic determination of lutein and zeaxanthin in aqueous humor from a single mouse eye.
To protect the eye from ultraviolet phototoxicity caused by free radicals, ocular components such as the aqueous humor accumulate antioxidants, such as the carotenoids. Lutein and zeaxanthin are the only carotenoids known to be present in the aqueous humor. Due to the small sample volume, pooling of samples from an undesirable large number of animals is often required for sufficient sensitivity and statistically significant differences to be achieved. In this paper we present a rapid, sensitive and robust packed capillary high-performance liquid chromatographic visible detection method for the quantification of lutein and zeaxanthin in the aqueous humor of single mouse eyes.
Karlsen
Anette
A
Institute for Nutrition Research, University of Oslo, N-0316 Oslo, Norway.
Alexander
George
G
Blomhoff
Rune
R
Gundersen
Thomas E
TE
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D014975:Lutein / Q000032:analysis*; D051379:Mice; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000032:analysis*
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D014975:Lutein