title: [Study on quantitative assay of chondroitin sulfate with a spectrophotometric method of azure A].
abstract: A simple, accurate and rapid spectrophotmeric method was proposed for the determination of chondroitin sulfate. The method was based on the absorbances of AA-CS complex at 625 nm being in proportion to the chondroitin sulfate concentrations. The linear range was 0-30 micrograms.mL-1 (R = 0.999), and the recovery range was 97.4%-103.8%. The quantities of chondroitin sulfate in different sample were determined in this way.
authors.affiliation: Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Gui-zhen
authors.initials: GZ
authors.lastname: Gao
authors.forename: Qing-cai
authors.initials: QC
authors.lastname: Jiao
authors.forename: Yi-lei
authors.initials: YL
authors.lastname: Ding
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
mesh_terms: D001399:Azure Stains / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000032:analysis* / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D001399:Azure Stains; D002614:Chelating Agents; D004365:Drugs, Chinese Herbal; D002809:Chondroitin Sulfates; C025818:Azure A