Structure-function studies of the lustrin A polyelectrolyte domains, RKSY and D4.
The lustrin superfamily represents a unique group of biomineralization proteins localized between layered aragonite mineral plates (i.e., nacre layers) in mollusk shell. These proteins not only exhibit elastomeric behavior within the mineralized matrix, but also adhesion to the aragonite-containing composite layer. One member of the lustrin superfamily, Lustrin A, has been sequenced; the protein is organized into defined, modular sequence domains that are hypothesized to perform separate functions (i.e., force unfolding, mineral adhesion, intermolecular binding) within the Lustrin A protein. Using nuclear magnetic resonance (NMR) and in vitro mineralization assays, we investigated structure-function relationships for two Lustrin A putative mineral binding domains, the 30 AA Arg, Lys, Tyr, Ser-rich (RKSY) and the 24 AA Asp-rich (D4) sequence regions domain of the Lustrin A protein. The results indicate that both sequences adopt open, unfolded structures that represent either extended or random coil states. Using geologic calcite overgrowth assays and scanning electron microscopic analyses, we observe that the RKSY polypeptide does not significantly perturb calcium carbonate growth. However, the D4 domain does influence crystal growth in a concentration-dependent manner. Collectively, our data indicate that D4, and not the RKSY domain, exhibits structure-function activity consistent with a mineral binding region.
Wustman
Brandon A
BA
Laboratory for Chemical Physics, New York University, New York 10010, USA.
Weaver
James C
JC
Morse
Daniel E
DE
Evans
John Spencer
JS
D000595:Amino Acid Sequence; D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002113:Calcification, Physiologic / Q000502:physiology*; D004573:Electrolytes / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D008974:Mollusca; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship
D001704:Biopolymers; D004573:Electrolytes; D016326:Extracellular Matrix Proteins; C109744:lustrin A