Genetic and cytological characterization of the RecA-homologous proteins Rad51 and Dmc1 of Schizosaccharomyces pombe.
The Schizosaccharomyces pombe rad51(+) and dmc1(+) genes code for homologues of the Escherichia coli recombination protein RecA. Deletion of rad51(+) causes slow growth, retardation of cell division and a decrease in viability. rad51Delta cells have a defect in mating-type switching. The DNA modification at the mating-type locus required for mating-type switching contributes to slow growth in the rad51 mutant. Cell mating is reduced in crosses homozygous for rad51Delta. Ectopic expression of the dmc1(+) gene allowed us to demonstrate that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dmc1- rad24 bicistronic RNA. We describe the functional defects of terminally epitope-tagged Dmc1 and Rad51 and discuss it in terms of protein interaction. Presumptive Rad51 and Dmc1 foci were detected on spreads of meiotic chromatin.
Grishchuk
Alexandra L
AL
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland.
Kraehenbuehl
Rolf
R
Molnar
Monika
M
Fleck
Oliver
O
Kohli
Juerg
J
D000251:Adenosine Triphosphatases; D000818:Animals; D000911:Antibodies, Monoclonal; D002451:Cell Compartmentation; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D003434:Crossing Over, Genetic; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D000939:Epitopes; D004926:Escherichia coli; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D007120:Immunochemistry; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D051135:Rad51 Recombinase; D011938:Rec A Recombinases / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C513032:Dmc1 protein, mouse; D000939:Epitopes; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; C084232:RHP51 protein, S pombe; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D051135:Rad51 Recombinase; C495154:Rad51 protein, mouse; D011938:Rec A Recombinases; D000251:Adenosine Triphosphatases