Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess.
A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.
Rovery
Clarisse
C
Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM, Marseille, France.
Rolain
Jean Marc
JM
Raoult
Didier
D
Brouqui
Philippe
P
D017347:Brucella melitensis / Q000302:isolation & purification*; D002498:Centrifugation; D002908:Chronic Disease; D006801:Humans; D008100:Liver Abscess / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics
D012336:RNA, Ribosomal, 16S