title: 8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini.
abstract: Nucleic acid damage by reactive nitrogen and oxygen species may contribute to the carcinogenesis associated with chronic infection and inflammation. We examined 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formation and nitric oxide (NO) production in hamsters infected with Opisthorchis viverrini (OV). Formation of 8-nitroguanine was assessed immunohistochemically with an antibody specific for 8-nitroguanine. 8-nitroguanine formation was found mainly in the cytoplasm and slightly in the nucleus of inflammatory cells and epithelial lining of bile duct at inflammatory areas in the liver. 8-nitroguanine immunoreactivity reached the highest intensity on day 30. A time profile of 8-nitroguanine formation was closely associated with that of plasma nitrate/nitrite. HPLC with an electrochemical detector revealed that the amount of 8-oxodG in the liver reached the maximal level on day 21. The mechanisms of 8-oxodG and 8-nitroguanine formation via O2*- and NO production triggered by OV infection were discussed in relation to cholangiocarcinoma development.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Pinlaor
authors.forename: Puangrat
authors.initials: P
authors.lastname: Yongvanit
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hiraku
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Reiji
authors.initials: R
authors.lastname: Semba
authors.forename: Shinji
authors.initials: S
authors.lastname: Oikawa
authors.forename: Mariko
authors.initials: M
authors.lastname: Murata
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kawanishi
mesh_terms: D000818:Animals; D006224:Cricetinae; D004249:DNA Damage; D018450:Disease Progression; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D008954:Models, Biological; D009569:Nitric Oxide / Q000096:biosynthesis; D009889:Opisthorchiasis / Q000378:metabolism* / Q000473:pathology
chemical_list: C095838:8-nitroguanine; D009569:Nitric Oxide; D006147:Guanine