title: Purification and cDNA cloning of a novel antibacterial peptide with a cysteine-stabilized alphabeta motif from the longicorn beetle, Acalolepta luxuriosa.
abstract: An antibacterial peptide from the hemolymph of a coleopteran insect, Acalolepta luxuriosa, in the superfamily Cerambyocidea was characterized. The mature antibacterial peptide had 27 amino acid residues with a theoretical molecular weight of 3099.29 and it showed antibacterial activity against Escherichia coli and Micrococcus luteus. The deduced amino acid sequence of the peptide showed that it had a cysteine-stabilized alphabeta motif with a C...CXXXC...C...CXC consensus sequence, like insect defensins. However, the results of a multiple sequence alignment and phylogenetic analysis with CLUSTAL X indicated that this peptide is a novel peptide with a cysteine-stabilized alphabeta motif that is distant from insect defensins.
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Nakamachi 2-24-16, Koganei, 184-8588, Tokyo, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Saito
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Ueda
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Nami
authors.initials: N
authors.lastname: Miura
authors.forename: Shogo
authors.initials: S
authors.lastname: Atsumi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tabunoki
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D001517:Coleoptera / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D003545:Cysteine / Q000737:chemistry*; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects; D006458:Hemolymph / Q000737:chemistry; D016982:Micrococcus luteus / Q000187:drug effects; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D000900:Anti-Bacterial Agents; D018076:DNA, Complementary; D010455:Peptides; D003545:Cysteine