Diethyldithiocarbamate inhibits the catalytic activity of xanthine oxidase.
We sought to determine the effects of the superoxide dismutase (SOD) inhibitor diethyldithiocarbamate (DETC) on vascular superoxide production. Rat aortic rings treated with DETC (10 mM) showed no change of superoxide generation (5 microM lucigenin). Likewise, DETC did not change the expression and activity of vascular soluble guanylyl cyclase, an enzyme known to be extremely sensitive to superoxide. In striking contrast, DETC completely inhibited the superoxide production induced by 6-anilino-5,8-quinolinedione (LY83583) and abolished the catalytic activity of xanthine oxidase (XO). Thus, DETC inhibits vascular superoxide production by blocking oxidoreductase enzymes such as XO and those reducing LY83583 in rat aorta.
Kober
Thorsten
T
Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum der Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
König
Ilona
I
Weber
Martina
M
Kojda
Georg
G
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000201:enzymology*; D002384:Catalysis; D046508:Culture Techniques; D004050:Ditiocarb / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004837:Epinephrine / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism*
D000634:Aminoquinolines; D004791:Enzyme Inhibitors; D013481:Superoxides; D019820:Xanthine; C041715:6-anilino-5,8-quinolinedione; D004050:Ditiocarb; D014969:Xanthine Oxidase; D006162:Guanylate Cyclase; D004837:Epinephrine