Gram-positive bacteria specific properties of silybin derived from Silybum marianum.
Silybin has a potent antibacterial activity, more potent than silymarin II, against gram-positive bacteria without hemolytic activity, whereas it has no antimicrobial activity against gram-negative bacteria or fungi. The mode of action of silybin against the gram-positive bacterial cell was examined by investigating the change in plasma membrane dynamics of bacterial cells using 1,6-diphenyl-1,3,5-hextriene (DPH) as a membrane probe and by assessing the inhibition of macromolecular synthesis using radiolabeled incorporation assay. The results showed that silybin inhibited RNA and protein synthesis on gram-positive bacteria.
Lee
Dong Gun
DG
Research Center for Proteineous Materials, Chosun University, Korea.
Kim
Hyung Keun
HK
Park
Yoonkyung
Y
Park
Seong-Cheol
SC
Woo
Eun-Rhan
ER
Jeong
Hye Gwang
HG
Hahm
Kyung-Soo
KS
D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D004912:Erythrocytes / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D020944:Silybum marianum / Q000737:chemistry*; D012329:RNA, Bacterial / Q000096:biosynthesis; D000077385:Silybin; D012838:Silymarin / Q000302:isolation & purification / Q000494:pharmacology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012838:Silymarin; D000077385:Silybin