Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line.
Previous studies have demonstrated that multidrug-resistant leukemic cells displayed nuclear texture changes. In this work, the human ovarian carcinoma cell line IGROV1 and its multidrug-resistant variant OV1/VCR were studied. Cell smears of these cell populations were analysed by image cytometry. As compared to sensitive cells, OV1/VCR display a chromatin global decondensation as assessed by textural features analysis. In order to correlate this decondensation with alterations in chromatin structure, DNase I was used. OV1/VCR DNA displayed an increased DNase I sensitivity, suggesting an increased chromatin accessibility. Furthermore, OV1/VCR cells displayed an increased level in acetylated histone H4, a mechanism known to be associated with transcriptionally active chromatin and relaxed chromatin conformation.
Yatouji
Sonia
S
Unité MéDIAN-CNRS UMR 6142, IFR 53, Faculté de Pharmacie, 51096 Reims cedex, France.
Trussardi-Regnier
Aurelie
A
Trentesaux
Chantal
C
Liautaud-Roger
Françoise
F
Dufer
Jean
J
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D006657:Histones / Q000737:chemistry; D006801:Humans; D019044:Image Cytometry; D015151:Immunoblotting; D010051:Ovarian Neoplasms / Q000473:pathology; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002843:Chromatin; D006657:Histones; D003850:Deoxyribonuclease I