title: Analysis of N-acyl-L-homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography--electrospray ionization-ion trap mass spectrometry.
abstract: A method for the analysis of N-acyl-L-homoserine lactones (AHLs) with micellar electrokinetic chromatography coupled to electrospray ionization-ion trap mass spectrometry, combining the flexibility of capillary electrophoresis with the unmatched structural information provided by mass spectrometry is presented. Different surfactants were evaluated, with sodium dodecyl sulfate (SDS) yielding the best results considering sensitivity and flexibility. We examined the interaction of AHLs with the SDS micelles at different analysis conditions and applied the optimized method to the analysis of a real bacterial sample. Two AHLs from Burkholderia cepacia colonizing the rhizosphere of traditional Indian rice cultivars could be unambiguously determined in an ethyl acetate extract with high resolution flexibility.
authors.affiliation: Institute of Ecological Chemistry, GSF--National Research Center for Environment and Health, Neuherberg, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Frommberger
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt-Kopplin
authors.forename: Franz
authors.initials: F
authors.lastname: Menzinger
authors.forename: Valerie
authors.initials: V
authors.lastname: Albrecht
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
authors.forename: Anton
authors.initials: A
authors.lastname: Hartmann
authors.forename: Antonius
authors.initials: A
authors.lastname: Kettrup
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis*; D016956:Burkholderia cepacia / Q000737:chemistry / Q000378:metabolism*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013501:Surface-Active Agents
chemical_list: D013501:Surface-Active Agents; C088386:homoserine lactone; D012967:Sodium Dodecyl Sulfate; D015107:4-Butyrolactone