Anti-endotoxic effects of syringic acid of Radix Isatidis.
The anti-endotoxic effect of syringic acid (SA) isolated from Radix Isatidis (Banlangen, BLG) was studied. SA was extracted and isolated from BLG and diluted into 1% solution. The content of SA-pretreated endotoxin (ET) was quantitatively determined using Limulus test. The ability of fever induction of ET pretreated with SA was measured using endotoxin-induced fever test in rabbits. The LPS-induced death in mice pretreated with and without SA was compared. Results showed that after pretreatment with SA, 83.16% of ET was destroyed, the ET-induced fever in rabbits relieved markedly and the LPS-induced death rate in mice dropped from 68% to 20%. It was concluded that SA isolated from BLG had anti-endotoxic effects.
Liu
Yunhai
Y
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Fang
Jianguo
J
Lei
Ting
T
Wang
Wenqing
W
Lin
Aihua
A
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019446:Endotoxemia / Q000097:blood; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D004926:Escherichia coli; D005260:Female; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D031219:Isatis / Q000737:chemistry*; D008033:Limulus Test; D008297:Male; D051379:Mice; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits
D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D005707:Gallic Acid; C001945:syringic acid