title: t(5;14)/HOX11L2-positive T-cell acute lymphoblastic leukemia. A collaborative study of the Groupe Français de Cytogénétique Hématologique (GFCH).
abstract: To accurately estimate the incidence of HOX11L2 expression, and determine the associated cytogenetic features, in T-cell acute lymphoblastic leukemia (T-ALL), the Groupe Français de Cytogénétique Hématologique (GFCH) carried out a retrospective study of both childhood and adult patients. In total, 364 patients were included (211 children </=15 years and 153 adults), and 67 (18.5%) [47 children (22.4%) and 20 adults (13.1%)] were shown to either harbor the t(5;14)q35;q32) translocation or express the HOX11L2 gene or both. Most of the common hematological parameters did not show significant differences within positive and negative populations, whereas the incidence of CD1a+/CD10+ and cytoplasmic CD3+ patients was significantly higher in positive than in negative children. Out of the 63 positive patients investigated by conventional cytogenetics, 32 exhibited normal karyotype, whereas the others 31 showed clonal chromosome abnormalities, which did not include classical T-ALL specific translocations. Involvement of the RANBP17/HOX11L2 locus was ascertained by fluorescence in situ hybridization in six variant or alternative (three-way translocation or cytogenetic partner other than 14q32) translocations out of the 223 patients. Our results also show that HOX11L2 expression essentially occurs as a result of a 5q35 rearrangement, but is not associated with another identified T-ALL specific recurrent genetic abnormality, such as SIL-TAL fusion or HOX11 expression.
authors.affiliation: EMI 0210, Hôpital Necker, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Berger
authors.forename: N
authors.initials: N
authors.lastname: Dastugue
authors.forename: M
authors.initials: M
authors.lastname: Busson
authors.forename: J
authors.initials: J
authors.lastname: Van Den Akker
authors.forename: C
authors.initials: C
authors.lastname: Pérot
authors.forename: P
authors.initials: P
authors.lastname: Ballerini
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: L
authors.initials: L
authors.lastname: Michaux
authors.forename: C
authors.initials: C
authors.lastname: Charrin
authors.forename: M P
authors.initials: MP
authors.lastname: Pages
authors.forename: F
authors.initials: F
authors.lastname: Mugneret
authors.forename: J
authors.initials: J
authors.lastname: Andrieux
authors.forename: P
authors.initials: P
authors.lastname: Talmant
authors.forename: C
authors.initials: C
authors.lastname: Hélias
authors.forename: L
authors.initials: L
authors.lastname: Mauvieux
authors.forename: M
authors.initials: M
authors.lastname: Lafage-Pochitaloff
authors.forename: M-J
authors.initials: MJ
authors.lastname: Mozziconacci
authors.forename: P
authors.initials: P
authors.lastname: Cornillet-Lefebvre
authors.forename: I
authors.initials: I
authors.lastname: Radford
authors.forename: V
authors.initials: V
authors.lastname: Asnafi
authors.forename: C
authors.initials: C
authors.lastname: Bilhou-Nabera
authors.forename: F
authors.initials: F
authors.lastname: Nguyen Khac
authors.forename: C
authors.initials: C
authors.lastname: Léonard
authors.forename: F
authors.initials: F
authors.lastname: Speleman
authors.forename: B
authors.initials: B
authors.lastname: Poppe
authors.forename: C
authors.initials: C
authors.lastname: Bastard
authors.forename: S
authors.initials: S
authors.lastname: Taviaux
authors.forename: B
authors.initials: B
authors.lastname: Quilichini
authors.forename: C
authors.initials: C
authors.lastname: Herens
authors.forename: M-J
authors.initials: MJ
authors.lastname: Grégoire
authors.forename: H
authors.initials: H
authors.lastname: Cavé
authors.forename: O A
authors.initials: OA
authors.lastname: Bernard
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D002999:Clone Cells; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D008297:Male; D015513:Oncogene Proteins / Q000235:genetics*; D011003:Ploidies; D011518:Proto-Oncogene Proteins; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014178:Translocation, Genetic
chemical_list: D018398:Homeodomain Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; C489494:TLX3 protein, human; C071250:TLX1 protein, human