Alteration in cellular and biochemical markers of pulmonary toxicity in rat lung exposed to carpet dusts.
Epidemiological studies of workers in weaving units in carpet industries have shown relationships between the airborne dust concentrations and pulmonary ill health. Therefore, to predict the health risk of carpet weavers, this preliminary experiment was conducted to evaluate the effect of carpet dust (knotted, tufted) on cellular and biochemical mediators considered as potential biological markers of lung injury. Lung cytoplasmic (lactate dehydrogenase, LDH), lysosomal (acid phosphatase, ACP), type II (alkaline phoshatase, ALP) and Clara-cell marker enzymes (gamma-glutamyl transferase, GGT) were monitored in rat cell-free lung lavage (BAL) during postexposure days 1, 4, 8, and 16. Furthermore, lung microsomal cytochrome P-450 (CYP450) and Clara-cell secretory protein (CC16) content in BAL was also evaluated. These pulmonary marker enzymes were significantly elevated during the postexposure period over the respective untreated control; however, tufted carpet dust shows more responses than knotted carpet dust. Lung CYP450 content was reduced significantly at early days; the pattern shows the reoccurrence of CYP450 content in the later stage of postexposure to carpet dust. Clara-cell secretory protein in BAL shows decline in the carpet-treated group; however, tufted carpet shows more decline than knotted carpet. Thus, reduction in CC16 level may have important implication in the development of chronic lung inflammation and diseases. Present investigation found that modulation of these cellular marker enzymes is clear evidence of pulmonary damage caused by exposure to carpet dust.
Ameen
Mohamed
M
Fibre Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Musthapa
Syed
S
Ahmad
Iqbal
I
Ansari
Furquan Ahmad
FA
Baig
Masroor Alam
MA
Rahman
Qamar
Q
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004391:Dust / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure / Q000009:adverse effects*; D005430:Floors and Floorcoverings; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000473:pathology; D008297:Male; D008861:Microsomes / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014598:Uteroglobin
D015415:Biomarkers; D004391:Dust; D011506:Proteins; C556391:Scgb1a1 protein, rat; D003577:Cytochrome P-450 Enzyme System; D014598:Uteroglobin; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase