Vacuolar accumulation and extracellular extrusion of electrophilic compounds by wild-type and glutathione-deficient mutants of the methylotrophic yeast Hansenula polymorpha.
The methylotrophic yeast Hansenula polymorpha CBS4732 leu2 detoxifies electrophilic xenobiotics by glutathione (GSH)-dependent accumulation in vacuoles, as shown by fluorescence microscopy. GSH-dependent and GSH-independent export of xenobiotic derivatives were also demonstrated by high-performance liquid chromatography (HPLC). Conjugates of GSH and N-acetylcysteine with monobromobimane and N-[1-pyrene]maleimide were observed among the HPLC fractions, along with unidentified derivatives.
Ubiyvovk
Vira M
VM
Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.
Maszewski
Janusz
J
Bartosz
Grzegorz
G
Sibirny
Andrei A
AA
D001692:Biological Transport; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D005978:Glutathione / Q000235:genetics / Q000502:physiology*; D008301:Maleimides / Q000378:metabolism; D009154:Mutation; D010843:Pichia / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure*; D015262:Xenobiotics / Q000378:metabolism
D001643:Bridged Bicyclo Compounds; D008301:Maleimides; D015262:Xenobiotics; C003944:N-(3-pyrene)maleimide; D005978:Glutathione; C028381:monobromobimane