title: Changes of plasma membrane ATPase activities and proline accumulation in twigs of Casuarina under different growth conditions.
abstract: Twigs of Casuarina were collected from Dongshan and Xiamen Island, China. Crude plasma membrane preparation was obtained from Casuarina twigs by ultra-centrifugation. Sealed plasma membrane vesicles were isolated with aqueous PEG3350-Dextran T500 two phase partitioning. We studied the proline accumulation in twigs and the ATPase activities of plasma membrane of Casurina under different growth conditions. The results showed that free proline content and the PM ATPase activities from unfavorable growth conditions were obviously higher than those from favorable environment. This suggested that Casuarina responded to osmotic stress by increasing osmotic material content and the proton transportation activities of PM ATPase.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361005.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Chong Ling
authors.initials: CL
authors.lastname: Yan
authors.forename: Yu Hong
authors.initials: YH
authors.lastname: Li
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D019684:Magnoliopsida / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012965:Sodium Chloride / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D012987:Soil / Q000032:analysis; D014887:Weather
chemical_list: D012987:Soil; D012965:Sodium Chloride; D011392:Proline; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase