Cluster stability scores for microarray data in cancer studies.
BACKGROUND
A potential benefit of profiling of tissue samples using microarrays is the generation of molecular fingerprints that will define subtypes of disease. Hierarchical clustering has been the primary analytical tool used to define disease subtypes from microarray experiments in cancer settings. Assessing cluster reliability poses a major complication in analyzing output from clustering procedures. While most work has focused on estimating the number of clusters in a dataset, the question of stability of individual-level clusters has not been addressed.


RESULTS
We address this problem by developing cluster stability scores using subsampling techniques. These scores exploit the redundancy in biologically discriminatory information on the chip. Our approach is generic and can be used with any clustering method. We propose procedures for calculating cluster stability scores for situations involving both known and unknown numbers of clusters. We also develop cluster-size adjusted stability scores. The method is illustrated by application to data three cancer studies; one involving childhood cancers, the second involving B-cell lymphoma, and the final is from a malignant melanoma study.


BACKGROUND
Code implementing the proposed analytic method can be obtained at the second author's website.
Smolkin
Mark
M
Department of Biostatistics, University of Michigan, Ann Arbor, Michigan, USA. Marksmolkin@hotmail.com
Ghosh
Debashis
D
D000465:Algorithms; D002648:Child; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data / Q000639:trends; D020869:Gene Expression Profiling / Q000379:methods* / Q000706:statistics & numerical data* / Q000639:trends; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics; D008545:Melanoma / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data* / Q000639:trends; D012512:Sarcoma, Ewing / Q000235:genetics