Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections.
We have developed a multiplex PCR procedure to determine the distribution of nine adhesin genes in Staphylococcus aureus isolates. Only genes encoding bone sialoprotein binding protein and fibronectin binding protein B were significantly associated with hematogenous osteomyelitis/arthritis and native-valve endocarditis, respectively, suggesting their involvement in hematogenous tissue infections.
Tristan
Anne
A
Centre National de Référence des Staphylocoques, INSERM E0230, IFR62 Laennec, 69372 Lyon Cédex 08, France.
Ying
Liu
L
Bes
Michele
M
Etienne
Jerome
J
Vandenesch
Francois
F
Lina
Gerard
G
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000502:physiology; D001170:Arthritis, Infectious / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D003030:Coagulase / Q000235:genetics; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D006801:Humans; D009666:Nose / Q000382:microbiology; D010019:Osteomyelitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; C048165:adhesin, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial