title: Mutations in katG, inhA, and ahpC genes of Brazilian isoniazid-resistant isolates of Mycobacterium tuberculosis.
abstract: The presence of mutations in specific regions of the katG, inhA, and ahpC genes was analyzed with 69 Mycobacterium tuberculosis isoniazid-resistant isolates from three Brazilian states. Point mutations in codon 315 of the katG gene were observed in 87.1, 60.9, and 60% of the isolates from Rio Grande do Sul, Rio de Janeiro, and São Paulo, respectively. Mutations in the inhA gene were identified only in one isolate from RJ State, and the ahpC promoter region revealed mutations in distinct positions in 12.9, 21.7, and 6.7% of the isolates from RS, RJ and SP, respectively.
authors.affiliation: Centro de Desenvolvimento Científico e Tecnológico, Laboratório Central de Saúde Pública, Fundação Estadual de Produção e Pesquisa em Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre-Rio Grande do Sul, Brazil.
authors.forename: Márcia Susana N
authors.initials: MS
authors.lastname: Silva
authors.forename: Simone G
authors.initials: SG
authors.lastname: Senna
authors.forename: Marta O
authors.initials: MO
authors.lastname: Ribeiro
authors.forename: Andréia R M
authors.initials: AR
authors.lastname: Valim
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: Telles
authors.forename: Afrânio
authors.initials: A
authors.lastname: Kritski
authors.forename: Glenn P
authors.initials: GP
authors.lastname: Morlock
authors.forename: Robert C
authors.initials: RC
authors.lastname: Cooksey
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
authors.forename: Maria Lucia R
authors.initials: ML
authors.lastname: Rossetti
mesh_terms: D001426:Bacterial Proteins; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics*; D054464:Peroxiredoxins; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; D010544:Peroxidases; D054464:Peroxiredoxins; C076136:catalase HPI; C085257:InhA protein, Mycobacterium; D007538:Isoniazid