Neurochemical analysis of brain monoamines after L-histidine and chlorpheniramine administration in goldfish.
This study investigated the effects of chlorpheniramine (CPA) and L-histidine (LH) administration on catecholaminergic levels in goldfish brain using neurochemical analysis. Fifty-eight animals were used. After 20 min of i.p. administration of the drugs or saline the animals were decapitated, and the telencephalon and the diencephalon were dissected. We also measured catecholamines in a non-injected (NI) group. Results showed lower homovanillic acid (HVA) levels after treatment with 100 mg/kg of LH when compared to saline and 5-hydroxyindoleacetic acid levels were lower in the saline group when compared to the NI group. In the diencephalon the NI group and animals treated with CPA at 4.0 and 8.0 mg/kg had lower HVA levels. Results suggest that LH had an inhibitory effect on dopaminergic activity and an anxiolytic-like effect for CPA results is suggested.
Medalha
Carla C
CC
Center of Biological Sciences and Health, Universidade Federal de São Carlos, SP, São Carlos, Brazil.
Santangelo
Erika M
EM
Mattioli
Rosana
R
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002744:Chlorpheniramine / Q000494:pharmacology*; D004027:Diencephalon / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013687:Telencephalon / Q000187:drug effects / Q000378:metabolism
D014151:Anti-Anxiety Agents; D015306:Biogenic Monoamines; D006634:Histamine H1 Antagonists; D012701:Serotonin; D002744:Chlorpheniramine; D006639:Histidine; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid