title: In vitro regulation of beta1 and beta3 integrin subunits in endometrial epithelial cells from normal endometrium.
abstract: OBJECTIVE
  To evaluate the effect of prostaglandin E2 (PGE2) and interleukin-1beta (IL-1beta) on integrin expression. DESIGN Cultures of endometrial epithelial cells from normal endometrium.
  
  
  METHODS
  All endometrial specimens were obtained from the Obstetrics and gynecology Department of the Catholic University, Rome, Italy.
  
  
  METHODS
  Four patients were normal menstrual cycles undergoing operative laparoscopy for non-endometrial problems.
  
  
  METHODS
  Endometrial samples were collected by uterine courettings.
  
  
  METHODS
  Immunocytochemistry for beta1 and beta3 integrin subunits.
  
  
  RESULTS
  PGE2 clearly enhances both beta1 and beta3 integrin subunit expression. IL-1beta seem to slightly increase only beta3 subunit expression.
  
  
  CONCLUSIONS
  In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE2 is also involved in local paracrine regulation of integrin expression.
authors.affiliation: Unità Operativa di Ginecologia ed Ostetricia, Ospedale G.B. Grassi, Roma, Italy
authors.forename: Emilia
authors.initials: E
authors.lastname: Pierro
authors.forename: Francesca
authors.initials: F
authors.lastname: Minici
authors.forename: Ornella
authors.initials: O
authors.lastname: Alesiani
authors.forename: Loredana D
authors.initials: LD
authors.lastname: Monica
authors.forename: Maria M
authors.initials: MM
authors.lastname: Anna
authors.forename: Salvatore
authors.initials: S
authors.lastname: Muncuso
authors.forename: Antonio
authors.initials: A
authors.lastname: Lanzone
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D015232:Dinoprostone / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology
chemical_list: D003470:Culture Media; D019012:Integrin beta1; D039661:Integrin beta3; D007375:Interleukin-1; D015232:Dinoprostone