Effects of RGD peptides on cells derived from the human eye.
OBJECTIVE
To investigate the effects of Arg-Gly-Asp (RGD) peptides on cells derived from the human eye.


METHODS
SV40-transformed human corneal epithelial cells (SCE), SV40-transformed human lens epithelial cells (SLE), normal human keratocytes (NK), and human retinal pigment epithelial cells stably expressing human telomerase reverse transcriptase (TRPE) were used. A human corneal cDNA library was screened to isolate the human homologue of p130 Crk-associated substrate (Cas). Next, after these cells were attached to the substratum, RGD-containing soluble tetrapeptides Arg-Gly-Asp-Ser (RGDS) were added to the culture medium and morphological changes were observed.


RESULTS
A clone, H-2, consisting of 3228 nucleotides, with a long open reading frame (870 amino acid residues) was isolated, and determined to be the human homologue of Cas. After addition of the RGDS peptides, both SLE and TRPE detached from the plastic culture plate, but SCE and NK did not. Detached SLE and TRPE showed decreased levels of tyrosine phosphorylation in Cas.


CONCLUSIONS
These results indicated that the signaling pathway through Cas played an important role in epithelial cell adhesion in the eye.
Imoto
Yoshiko
Y
Department of Ophthalmology, Osaka University Medical School, Osaka, Japan.
Ohguro
Nobuyuki
N
Yoshida
Atsushi
A
Tsujikawa
Motokazu
M
Inoue
Yoshitsugu
Y
Tano
Yasuo
Y
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002461:Cell Line, Transformed; D029563:Cellular Apoptosis Susceptibility Protein / Q000235:genetics / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D004268:DNA-Binding Proteins; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D019098:Telomerase / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D029563:Cellular Apoptosis Susceptibility Protein; D004268:DNA-Binding Proteins; D016023:Integrins; D009842:Oligopeptides; D010446:Peptide Fragments; D014443:Tyrosine; C047981:arginyl-glycyl-aspartic acid; D019098:Telomerase