title: Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess.
abstract: A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.
authors.affiliation: Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM, Marseille, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Rovery
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Rolain
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Philippe
authors.initials: P
authors.lastname: Brouqui
mesh_terms: D017347:Brucella melitensis / Q000302:isolation & purification*; D002498:Centrifugation; D002908:Chronic Disease; D006801:Humans; D008100:Liver Abscess / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S