title: Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method and a dual-round polymerase chain reaction.
abstract: Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.
authors.affiliation: Department of Forensic Medical Science, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-ku, Japan. nagaom@med.nagoya-cu.ac.jp
authors.forename: Mineo
authors.initials: M
authors.lastname: Iwasa
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Koyama
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Tsuchimochi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Maeno
authors.forename: Ichiro
authors.initials: I
authors.lastname: Isobe
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Seko-Nakamura
authors.forename: Jun
authors.initials: J
authors.lastname: Monma-Ohtaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Nagao
mesh_terms: D000328:Adult; D002614:Chelating Agents; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology; D020080:Tandem Repeat Sequences; D014621:Vagina / Q000166:cytology; D014626:Vaginal Smears
chemical_list: D002614:Chelating Agents; D004247:DNA