Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis.
Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-gamma production by CD8+ T cells from HLA-A*0201+ healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.
Shams
Homayoun
H
Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, 11937 U.S. Hwy. 271, Tyler, TX 75708-3154, USA. amir.shams@uthct.edu
Barnes
Peter F
PF
Weis
Stephen E
SE
Klucar
Peter
P
Wizel
Benjamin
B
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015234:HLA-A Antigens / Q000378:metabolism; D015789:HLA-A2 Antigen; D006460:Hemolysin Proteins / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000942:Antigens, Bacterial; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D006460:Hemolysin Proteins; D010446:Peptide Fragments; C072681:Mycobacterium tuberculosis antigens