title: The synergistic effect of TGF-beta and 1,25-dihydroxyvitamin D3 on SPARC synthesis and alkaline phosphatase activity in human pulp fibroblasts.
abstract: 1,25(OH)2D3 and TGF-beta can influence the function and differentiation of dental pulp fibroblasts. In this study, we examined the effect of 1,25(OH)2D3 and TGF-beta on the synthesis of SPARC and ALP activity in human pulp fibroblasts. Two isoforms of SPARC, the 43 and 38 kDa, were detected in this cell type. TGF-beta increased the synthesis of SPARC about 2.5-fold after 3 days of treatment but had no effect on the ALP activity. On the contrary, 1,25(OH)2D3 increased ALP activity 2-fold but had no effect on SPARC. The combination of TGF-beta and 1,25(OH)2D3 significantly induced SPARC synthesis and ALP activity by 5 and 9 folds, respectively (P<0.05). This finding suggested the synergistic effect between TGF-beta and 1,25(OH)2D3 in dental pulp fibroblasts on the synthesis of SPARC and ALP activity. This interaction could influence the function and differentiation of dental pulp fibroblasts.
authors.affiliation: Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Henri Dunant Road, Pathumwan, Bangkok 10330, Thailand. prasit.pav@chula.ac.th
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.forename: Tussanee
authors.initials: T
authors.lastname: Yongchaitrakul
authors.forename: Kassara
authors.initials: K
authors.lastname: Pattamapun
authors.forename: Mansuang
authors.initials: M
authors.lastname: Arksornnukit
mesh_terms: D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D002478:Cells, Cultured; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D007536:Isomerism; D008963:Molar / Q000378:metabolism; D015676:Osteonectin / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D015676:Osteonectin; D016212:Transforming Growth Factor beta; D015650:24,25-Dihydroxyvitamin D 3; D000469:Alkaline Phosphatase