title: Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum.
abstract: Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the alpha-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.
authors.affiliation: Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 55108-6010, USA.
authors.forename: Benedict T
authors.initials: BT
authors.lastname: Green
authors.forename: Mark
authors.initials: M
authors.lastname: Lyte
authors.forename: Anjali
authors.initials: A
authors.lastname: Kulkarni-Narla
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D005260:Female; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation / Q000382:microbiology; D007583:Jejunum / Q000187:drug effects / Q000294:innervation* / Q000382:microbiology*; D008297:Male; D015213:Neuroimmunomodulation / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D010581:Peyer's Patches / Q000187:drug effects / Q000294:innervation* / Q000382:microbiology*; D019779:Salmonella enterica / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D012530:Saxitoxin / Q000494:pharmacology; D013552:Swine
chemical_list: D012530:Saxitoxin; D009638:Norepinephrine