title: Haemocytes from Pseudoplusia includens express multiple alpha and beta integrin subunits.
abstract: Cellular immune responses such as encapsulation involve the adhesion of one or more classes of haemocytes. How insect haemocytes recognize encapsulation targets as foreign or the identity of the molecules regulating haemocyte adhesion is unknown. One of the most important classes of adhesion receptors in mammalian immune cells is the integrins, which form functional heterodimers through different combinations of alpha and beta subunits. Prior studies with the moth Pseudoplusia includens indicated that encapsulation depends on two classes of haemocytes called granulocytes and plasmatocytes. Here we report the cloning and identification of three alpha integrin subunits (alphaPi1-3) and one beta subunit (betaPi1) from P. includens. Northern blot analysis indicated that all four subunits are expressed in granulocytes and that three of the four subunits are expressed in plasmatocytes. Quantification of transcription patterns using real-time PCR revealed that expression of alphaPi2 and betaPi1 increased in granulocytes and plasmatocytes when binding to a foreign surface or forming a capsule. alphaPi2 transcription in plasmatocytes was further increased by granulocyte conditioned medium, plasmatocyte spreading peptide, and the integrin recognition peptide RGD. Collectively, these results suggest that one or more integrins play an important role in regulating haemocyte adhesion during encapsulation.
authors.affiliation: Department of Entomology, University of Georgia, Athens, GA, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Lavine
authors.forename: M R
authors.initials: MR
authors.lastname: Strand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D017931:DNA Primers; D006434:Hemocytes / Q000276:immunology*; D039001:Integrin alpha Chains / Q000235:genetics* / Q000276:immunology; D039641:Integrin beta Chains / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D039001:Integrin alpha Chains; D039641:Integrin beta Chains