Isolation and characterization of novel halotolerant and/or halophilic denitrifying bacteria with versatile metabolic pathways for the degradation of trimethylamine.
Four denitrifying bacteria capable of degrading trimethylamine under both aerobic and denitrifying conditions were newly isolated from coastal sediments and wastewater contaminated by marine water. All strains were in alpha-Proteobacteria. Strain GP43 was classified as a member of genus Paracoccus, and strain PH32, PH34 and GRP21 were novel organisms with remote phylogenetic position from other genus alpha-Proteobacteria. Among these four strains were the halophilic strains PH32, PH34 and GRP21, which did not grow in the absence of sodium chloride in culture medium. Cells grown under denitrifying conditions possessed trimethylamine dehydrogenase while cells grown aerobically possessed two different enzymes for oxidation of trimethylamine, trimethylamine dehydrogenase and trimethylamine monooxygenase. The newly isolated strain PH32, PH34 and GRP21 may be the first halophilic bacteria to degrade trimethylamine under denitrifying conditions.
Kim
Song-Gun
SG
Environmental Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Gusong-Dong, Yusung-Gu, Daejeon 305-701, South Korea.
Bae
Hee-Sung
HS
Oh
Hee-Mock
HM
Lee
Sung-Taik
ST
D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D000693:Anaerobiosis; D001483:Base Sequence; D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000737:chemistry; D008744:Methylamines / Q000378:metabolism*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010089:Oxidoreductases, N-Demethylating / Q000502:physiology; D010105:Oxygenases / Q000502:physiology; D010230:Paracoccus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D017385:Sequence Homology; D012965:Sodium Chloride / Q000378:metabolism* / Q000494:pharmacology*; D014871:Water Microbiology
D004275:DNA, Ribosomal; D008744:Methylamines; D009566:Nitrates; D012965:Sodium Chloride; D010105:Oxygenases; C043996:tertiary amine monooxygenase; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; C023336:trimethylamine