title: The impact of intrapartum factors on umbilical cord blood stem cell banking.
abstract: OBJECTIVE
  Umbilical cord blood can be used as an alternative source for related and unrelated allogeneic stem cell transplantation. This study was undertaken to determine whether intrapartum factors have an influence on the hematopoietic cell compartment of cord blood.
  
  
  METHODS
  Cord blood samples were obtained from 102 normal full-term deliveries for the banking of stem cells. We analyzed the influence of intrapartum factors on the count of CD34+ cells, total nucleated cells, colony forming units and total volume of collection. Fluorescence-activated cell sorting was used to measure CD34+ cell numbers. Statistical analysis was undertaken using Pearson correlation test and multiple regression analysis.
  
  
  RESULTS
  The higher the infants' birthweight the larger was the volume. A lower arterial umbilical pH and a larger blood volume resulted in an increased number of CD34+ cells. A large blood volume, long duration of labor, lower arterial and venous pH were correlated with more nucleated cells. A higher birthweight, larger blood volume and lower arterial pH resulted in an increased number of colony forming units.
  
  
  CONCLUSIONS
  Some intrapartum factors have an impact on the characteristics of collected cord blood cells. Stress during delivery may influence the number of hematopoetic cells, through altered cytokine production. This knowledge may facilitate the selection of optimal cord blood samples for unrelated banking and the early discarding of suboptimal cord blood samples thus resulting in the saving of costs related to expensive further processing.
authors.affiliation: Department of Obstetric and Gynecology, University of Basel, Switzerland.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Aufderhaar
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holzgreve
authors.forename: Enrico
authors.initials: E
authors.lastname: Danzer
authors.forename: André
authors.initials: A
authors.lastname: Tichelli
authors.forename: Carolyn
authors.initials: C
authors.lastname: Troeger
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Surbek
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D001724:Birth Weight / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods*; D001810:Blood Volume / Q000502:physiology; D002452:Cell Count; D002585:Cesarean Section; D036861:Delivery, Obstetric / Q000379:methods*; D004900:Erythroblasts / Q000166:cytology; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D015999:Multivariate Analysis; D010298:Parity; D011247:Pregnancy; D013997:Time Factors
chemical_list: D018952:Antigens, CD34