Morphology of the type strain of Bacillus anthracis EY 3169T=ATCC 14578T grown either aerobically or anaerobically on agar plates--observation by light and laser microscopes.
Growth characteristics including cell-arrangement of the type strain of Bacillus anthracis EY 3169T=ATCC 14578T grown on agar plates in level 3 laboratory were observed by both light and laser microscopes. Small daughter colonies appeared on parent colonies grown on 5% sheep blood or chocolate agar plates after 12 days incubation at room temperature. Daughter colonies, stained by Wirtz-Conklin method, were composed with vegetative cells and spores. Growth of daughter colonies might be supported by the debris of cells in the parent colony. Colonies grown under anaerobic conditions were flat with smooth edges, and the cells neither formed chains of any length, nor produced any spores after 25 days incubation at room temperature. It was thought that spores of B. anthracis were produced at the terminal stage of individual cell life instead of under unfavorable conditions for the organism. Air is needed for spore formation and cell-chain formation. More nutrients, probably amino acids, are needed for anaerobic growth rather than aerobic.
Yabuuchi
Eiko
E
Department of Microbiology, Gifu University School of Medicine, Gifu, Gifu 500-8705, Japan. achei08@k2.dion.ne.jp
Koseki
Masayuki
M
D000332:Aerobiosis; D000693:Anaerobiosis; D001408:Bacillus anthracis / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D007834:Lasers; D008853:Microscopy; D018613:Microscopy, Confocal; D013171:Spores, Bacterial / Q000166:cytology / Q000378:metabolism
D003470:Culture Media