title: Effects of sodium magnesium fructose diphosphate on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome.
abstract: OBJECTIVE
  To study the effects of sodium magnesium fructose diphosphate (SMFD) on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome, so as to explore the protective mechanisms of SMFD on cerebral ischemia.
  
  
  METHODS
  The synaptosomes from normal rat brain were prepared by phase partition and cultured with oxygen-glucose deprivation to establish ischemic synaptosome model. The intrasynaptosomal free calcium concentration and nitric oxide synthase activity were detected separately after the synaptosomes were co-incubated with SMFD (1.3 mmol.L-1) or fructose-1, 6-diphosphate (FDP, 4.0 mmol.L-1) for 60 min.
  
  
  RESULTS
  SMFD decreased the free calcium concentration and reduced the activity of nitric oxide synthase (NOS) of ischemic synaptosomes. Its effects were more powerful than those of FDP.
  
  
  CONCLUSIONS
  SMFD may protect neurons from ischemic injury by preventing intracellular Ca2+ overload and inhibiting the activity of nitric oxide synthase.
authors.affiliation: Department of Pharmacology and Toxicology, Chongqing Pharmaceutical Research Institute, Chongqing 400061, China. zfx2806@sina.com
authors.forename: Fan-xin
authors.initials: FX
authors.lastname: Zeng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dong
authors.forename: Qi-xin
authors.initials: QX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology; D005635:Fructosediphosphates / Q000494:pharmacology*; D008274:Magnesium / Q000737:chemistry; D008297:Male; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000737:chemistry; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D005635:Fructosediphosphates; D012964:Sodium; D019001:Nitric Oxide Synthase; D008274:Magnesium; C029063:fructose-1,6-diphosphate; D002118:Calcium