Molecular cloning of canine RCAS1 cDNA.
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen, strongly expressed in invasive cancers. RCAS1 inhibited the in vitro growth of immunocytes, and induced apoptotic cell death. The cloning of canine RCAS1 cDNA was carried out and identified from the mammary gland tumor of a dog. A canine RCAS1 cDNA of 864 bp in length has an open reading frame of 642 bp nucleotides encoding a protein of 213 deduced amino acids. The predicted amino acid sequence of canine RCAS1 showed 96.2% and 96.7% homologies with those of human and mouse RCAS1 respectively. Canine RCAS1 has an N-terminal transmembrane segment and a coiled-coil structure in the C-terminal protein, which are highly conserved in mouse and human RCAS1.
Okamura
Yasuhiko
Y
Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan.
Ma
Zhiyong
Z
Khatlani
Tanvir S
TS
Okuda
Masaru
M
Une
Satoshi
S
Nakaichi
Munekazu
M
Taura
Yasuho
Y
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D005260:Female; D006801:Humans; D007107:Immune System / Q000502:physiology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000951:Antigens, Neoplasm; D017931:DNA Primers; D018076:DNA, Complementary