Lack of association between interleukin-10 gene promoter polymorphism, -1082G/A, and severe malaria in Thailand.
Interleukin-10 (IL-10) is an important cytokine in the down-regulation of inflammatory responses, and it has been reported that a low plasma concentration of IL-10 is associated with severe anemia and cerebral malaria in Plasmodium falciparum infections. The IL-10 gene is located on chromosome 1q31-32, and a promoter polymorphism (-1082G/A) is known to affect IL-10 protein production. In order to examine the possible association of the -1082G/A polymorphism with the severity of malaria, we studied 203 mild malaria, 164 non-cerebral severe malaria, and 109 cerebral malaria patients living in northwest Thailand. The genotyping was performed by a fluorescence resonance energy transfer (FRET) method. The frequencies of a major allele -1082A in mild malaria, in non-cerebral severe malaria, and in cerebral malaria patients were 92.6%, 92.1%, and 92.7% respectively. Our results showed no significant association of the -1082G/A polymorphism with the severity of malaria.
Ohashi
Jun
J
Department of Human Genetics, Graduate School of Medicine, University of Tokyo, Japan. juno@m.u-tokyo.ac.jp
Naka
Izumi
I
Patarapotikul
Jintana
J
Hananantachai
Hathairad
H
Looareesuwan
Sornchai
S
Tokunaga
Katsushi
K
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016778:Malaria, Falciparum / Q000235:genetics* / Q000503:physiopathology; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012720:Severity of Illness Index; D013785:Thailand
D017931:DNA Primers; D016753:Interleukin-10