Enhanced enzymatic synthesis of a semi-synthetic cephalosprin, cefaclor, with in situ product removal.
In the enzymatic synthesis of cefaclor, 3-chloro-7-d-(2-phenylglycinamide)-3-cephem-4-carboxylic acid, from phenylglycine methyl ester and 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid, the in situ product could influence both the overall conversion and hydrolysis of the ester. Optimization of the parameters, such as pH 6.2, 5 degrees C and substrate molar ratio of 2:1, made in situ product removal improve the overall conversion from 64% to 85% (mol/mol).
Yang
Liu
L
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, P.R. China.
Wei
Dong-Zhi
DZ
D019149:Bioreactors; D002433:Cefaclor / Q000138:chemical synthesis* / Q000737:chemistry; D002511:Cephalosporins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008956:Models, Chemical; D010399:Penicillin Amidase / Q000378:metabolism*; D013053:Spectrophotometry; D013379:Substrate Specificity
D002511:Cephalosporins; D004800:Enzymes, Immobilized; C033393:methyl phenylglycine; D002433:Cefaclor; C014320:7-aminodesacetoxycephalosporanic acid; D010399:Penicillin Amidase; D005998:Glycine