title: Experimental colonic obstruction increases collagen degradation by matrix metalloproteinases in the bowel wall.
abstract: OBJECTIVE
  Emergency resections for colonic obstruction are accompanied with increased risk of anastomotic dehiscence. Elevated local degradation of submucosal collagens by matrix metalloproteinases may predispose to anastomotic leakage. This study was designed to study the effect of colon obstruction and surgical trauma on matrix metalloproteinase activities and correlate these results to collagen concentration in the colon wall.
  
  
  METHODS
  Colonic obstruction was induced in male, Sprague-Dawley rats (n = 58) by applying a constricting silicone ring around the left colon 3 cm above the peritoneal reflection. After four days of obstruction, 2-mm wide colonic segments were resected approximately 3 mm proximal and 3 mm distal to the stenosis for biochemical analyses. Colonic segments at corresponding locations were obtained from sham-operated rats (n = 5) without obstruction but with silicone ring placed adjacent to colon and from normal, nontraumatized rats (n = 10). Matrix metalloproteinase activity was determined by liberation of fragmented collagens from homogenized colonic tissue incubated ex vivo. Matrix metalloproteinase-2 specifically was analyzed by gelatin zymography.
  
  
  RESULTS
  Endogenous collagenolysis by matrix metalloproteinases increased (P < 0.001) in colon as a consequence of obstruction (4.1-fold) and trauma (1.7-fold) compared with normal colon. In the proximity of the colon stenosis, total matrix metalloproteinase activity and matrix metalloproteinase-2 were significantly (P < 0.05) higher above than below the obstruction. Total activity was 22.9 (13.1-32.9) units/mg collagen proximal and 16.6 (12.7-18.4) units/mg collagen distal to the stenosis. Collagen concentration correlated inversely (r = -0.76; P < 0.001) with total matrix metalloproteinase activity.
  
  
  CONCLUSIONS
  Colonic obstruction and trauma up-regulated matrix metalloproteinases and decreased collagen concentration in colonic wall.
authors.affiliation: Department of Surgery, Malmö University Hospital, Lund University, Malmö, Sweden.
authors.forename: Ingvar
authors.initials: I
authors.lastname: Syk
authors.forename: Ursula
authors.initials: U
authors.lastname: Mirastschijski
authors.forename: Bengt W
authors.initials: BW
authors.lastname: Jeppsson
authors.forename: Magnus S
authors.initials: MS
authors.lastname: Agren
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003106:Colon / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006909:Hydroxyproline / Q000032:analysis; D007415:Intestinal Obstruction / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D023421:Models, Animal; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003094:Collagen; D009195:Peroxidase; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D006909:Hydroxyproline