Citrus psorosis virus RNA 1 is of negative polarity and potentially encodes in its complementary strand a 24K protein of unknown function and 280K putative RNA dependent RNA polymerase.
Citrus psorosis virus (CPsV), the type member of genus Ophiovirus, has three genomic RNAs. Complete sequencing of CPsV RNA 1 revealed a size of 8184 nucleotides and Northern blot hybridization with chain specific probes showed that its non-coding strand is preferentially encapsidated. The complementary strand of RNA 1 contains two open reading frames (ORFs) separated by a 109-nt intergenic region, one located near the 5'-end potentially encoding a 24K protein of unknown function, and another of 280K containing the core polymerase motifs characteristic of viral RNA-dependent RNA polymerases (RdRp). Comparison of the core RdRp motifs of negative-stranded RNA viruses, supports grouping CPsV, Ranunculus white mottle virus (RWMV) and Mirafiori lettuce virus (MiLV) within the same genus (Ophiovirus), constituting a monophyletic group separated from all other negative-stranded RNA viruses. Furthermore, RNAs 1 of MiLV, CPsV and RWMV are similar in size and those of MiLV and CPsV also in genomic organization and sequence.
Naum-Onganía
Gabriela
G
Facultad de Ciencias Exactas, Instituto de Bioquímica y Biología Molecular, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina.
Gago-Zachert
Selma
S
Peña
Eduardo
E
Grau
Oscar
O
Garcia
Maria Laura
ML
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002957:Citrus / Q000821:virology*; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000737:chemistry / Q000235:genetics; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*
D012324:RNA-Dependent RNA Polymerase