title: Structural analysis and genetic variation of the 16S-23S rDNA internal spacer region from Micrococcus luteus strains.
abstract: OBJECTIVE
  To clone and sequence the 16S-23S ribosomal DNA (rDNA) internal spacer region (ISR) from Micrococcus luteus.
  
  
  RESULTS
  The primer pair for 16S-23S rDNA ISR amplified a fragment of about 850 bp in length for two strains, JCM3347 and JCM3348 and a fragment of about 790 bp for a strain, ATCC9341. After sequencing the ISRs were identified by the comparison of the ISRs and the flanking regions of ISR.
  
  
  CONCLUSIONS
  Although the sequence difference of the ISR occurred at only one position between the two JCM strains, the highly variable length (440 and 370 bp) and sequence similarity (about 40%) were demonstrated between the ISRs of the two JCM strains and a ATCC strain.
  
  
  CONCLUSIONS
  A CCTCCT sequence was first detected at the 3'-end of the 16S rDNA of the three strains. Moreover, highly similar sequence to the 21-bp region containing a putative rRNA processing site was observed in the ISR of the three strains. Interestingly, no intercistronic tRNAs were demonstrated in the ISRs from the three strains.
authors.affiliation: Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Haga
authors.forename: Y
authors.initials: Y
authors.lastname: Hirano
authors.forename: O
authors.initials: O
authors.lastname: Murayama
authors.forename: B C
authors.initials: BC
authors.lastname: Millar
authors.forename: J E
authors.initials: JE
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D020459:Genes, rRNA; D014644:Genetic Variation; D016982:Micrococcus luteus / Q000235:genetics*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S