Upregulation of uncoupling proteins by oral administration of capsiate, a nonpungent capsaicin analog.
Capsiate is a nonpungent capsaicin analog, a recently identified principle of the nonpungent red pepper cultivar CH-19 Sweet. In the present study, we report that 2-wk treatment of capsiate increased metabolic rate and promoted fat oxidation at rest, suggesting that capsiate may prevent obesity. To explain these effects, at least in part, we examined uncoupling proteins (UCPs) and thyroid hormones. UCPs and thyroid hormones play important roles in energy expenditure, the maintenance of body weight, and thermoregulation. Two-week treatment of capsiate increased the levels of UCP1 protein and mRNA in brown adipose tissue and UCP2 mRNA in white adipose tissue. This dose of capsiate did not change serum triiodothyronine or thyroxine levels. A single dose of capsiate temporarily raised both UCP1 mRNA in brown adipose tissue and UCP3 mRNA in skeletal muscle. These results suggest that UCP1 and UCP2 may contribute to the promotion of energy metabolism by capsiate, but that thyroid hormones do not.
Masuda
Yoriko
Y
Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto Univ., Kyoto 606-8502, Japan.
Haramizu
Satoshi
S
Oki
Kasumi
K
Ohnuki
Koichiro
K
Watanabe
Tatsuo
T
Yazawa
Susumu
S
Kawada
Teruo
T
Hashizume
Shu-ichi
S
Fushiki
Tohru
T
D000273:Adipose Tissue / Q000187:drug effects; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism; D005740:Gases / Q000378:metabolism; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D051379:Mice; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D018482:Muscle, Skeletal / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013963:Thyroid Hormones / Q000097:blood; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D015854:Up-Regulation / Q000187:drug effects*
D002352:Carrier Proteins; D005740:Gases; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D013963:Thyroid Hormones; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; C000604393:Ucp3 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D012313:RNA; C470213:capsiate; D002211:Capsaicin