title: Experimental results of donor site filling for autologous osteochondral mosaicplasty.
abstract: OBJECTIVE
  Autologous osteochondral mosaicplasty has become a treatment option for focal chondral and osteochondral defects in recent years. Excessive postoperative bleeding from the donor site was reported as a possible complication of the procedure. The purpose of this study was to investigate different biodegradable materials for donor site filling, which could prevent excessive postoperative bleeding from these sites but would allow reasonable blood clot formation on the articular surfaces of donor tunnels.
  
  
  METHODS
  Basic science evaluation.
  
  
  METHODS
  In an experimental model, "donor site plugs" made from hydroxylapatite, carbon fiber, polyglyconate-B, compressed collagen, and 2 versions of polycaprolactones were used to fill the bony tunnels created by harvesting. These materials were tested in 100 knees of 50 German Shepherd dogs to determine the quality of the repair tissue formation on the surfaces of the harvesting holes filled by these materials. Arthroscopies of the dogs were performed at several intervals, from 4 weeks to 26 weeks, and macroscopic studies were performed on euthanized animals between 8 weeks and 30 weeks to evaluate donor site filling and coverage. Empty donor tunnels served as controls for the evaluation of the different filling materials.
  
  
  RESULTS
  All tested materials effectively decreased postoperative bleeding. Hydroxylapatite, carbon rods, polyglyconate-B, and melted polycaprolactone materials showed a good integration to the surrounding cancellous bone, but these fillings showed only a limited repair tissue formation, even at 30 weeks postoperatively. Second-look arthroscopy and histologic evaluation of necropsies showed the best fibrocartilage coverage after filling by compressed collagen. Technical details of the filling also had certain importance in the quality of the repair tissue formation.
  
  
  CONCLUSIONS
  According to histologic results, compressed collagen appears to be a good material to fill donor tunnels of osteochondral graft harvest. This material is substituted gradually by bone formation and its articular surface can serve as an appropriate scaffold for fibrocartilage coverage created by the natural intrinsic repair process.
authors.affiliation: Orthopaedic and Trauma Department Uzsoki Hospital, Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Feczkó
authors.forename: László
authors.initials: L
authors.lastname: Hangody
authors.forename: József
authors.initials: J
authors.lastname: Varga
authors.forename: Lajos
authors.initials: L
authors.lastname: Bartha
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Diószegi
authors.forename: Gabor
authors.initials: G
authors.lastname: Bodó
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Kendik
authors.forename: László
authors.initials: L
authors.lastname: Módis
mesh_terms: D000818:Animals; D001182:Arthroscopy; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001777:Blood Coagulation; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation; D002244:Carbon; D000077482:Carbon Fiber; D002358:Cartilage, Articular / Q000601:surgery / Q000637:transplantation*; D003094:Collagen; D004285:Dogs; D017886:Durapatite; D005260:Female; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D016348:Osseointegration; D011091:Polyesters; D011108:Polymers; D019106:Postoperative Hemorrhage / Q000517:prevention & control*; D020858:Tissue and Organ Harvesting / Q000009:adverse effects*; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D000077482:Carbon Fiber; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D002244:Carbon; C046409:polyglyconate; D003094:Collagen; D017886:Durapatite