title: Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA.
abstract: We established an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of bovine macrophage colony-stimulating factor (M-CSF) and used it to measure the serum M-CSF levels in bovine fetuses and calves. The average serum M-CSF level was 2.7+/-1.5 ng/ml in 39 calves under 100 days old, and 1.8+/-0.8 ng/ml in 15 cattle between 101 and 418 days old. Fetal sera samples (n = 6) prepared from cattle between 150 and 280 days of gestational age had a higher average level of M-CSF (8.8+/-1.4 ng/ml). Alteration in serum M-CSF levels in each individual calf was also measured. The serum levels of M-CSF in calves at 0-1 day after birth ranged from 0.52 to 7.3 ng/ml. During the period 113-125 days after birth, serum levels were around 1.4+/-0.39 ng/ml. Although serum M-CSF levels generally decreased as the age of calves advanced, differences among individuals, especially among newborn calves, were observed.
authors.affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. kazukazu@affrc.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Oshima
authors.forename: Y
authors.initials: Y
authors.lastname: Muneta
authors.forename: R
authors.initials: R
authors.lastname: Kikuma
authors.forename: C
authors.initials: C
authors.lastname: Yayota
authors.forename: T
authors.initials: T
authors.lastname: Hirai
authors.forename: N
authors.initials: N
authors.lastname: Satoh
authors.forename: S
authors.initials: S
authors.lastname: Matsuura
authors.forename: Y
authors.initials: Y
authors.lastname: Kikyo
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: C
authors.initials: C
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Inumaru
authors.forename: Y
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002417:Cattle / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005333:Fetus; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D011247:Pregnancy; D012680:Sensitivity and Specificity
chemical_list: D016173:Macrophage Colony-Stimulating Factor