title: Vacuolar accumulation and extracellular extrusion of electrophilic compounds by wild-type and glutathione-deficient mutants of the methylotrophic yeast Hansenula polymorpha.
abstract: The methylotrophic yeast Hansenula polymorpha CBS4732 leu2 detoxifies electrophilic xenobiotics by glutathione (GSH)-dependent accumulation in vacuoles, as shown by fluorescence microscopy. GSH-dependent and GSH-independent export of xenobiotic derivatives were also demonstrated by high-performance liquid chromatography (HPLC). Conjugates of GSH and N-acetylcysteine with monobromobimane and N-[1-pyrene]maleimide were observed among the HPLC fractions, along with unidentified derivatives.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.
authors.forename: Vira M
authors.initials: VM
authors.lastname: Ubiyvovk
authors.forename: Janusz
authors.initials: J
authors.lastname: Maszewski
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Sibirny
mesh_terms: D001692:Biological Transport; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D005978:Glutathione / Q000235:genetics / Q000502:physiology*; D008301:Maleimides / Q000378:metabolism; D009154:Mutation; D010843:Pichia / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure*; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D001643:Bridged Bicyclo Compounds; D008301:Maleimides; D015262:Xenobiotics; C003944:N-(3-pyrene)maleimide; D005978:Glutathione; C028381:monobromobimane