title: Mycobiota associated with the coffee berry borer (Hypothenemus hampei) in Mexico.
abstract: Field surveys were carried out in coffee plantations in Chiapas, Mexico, to collect and identify fungi associated with the cuticle, gut, faeces and galleries of the coffee berry borer, Hypothenemus hampei. Insects and coffee berries containing galleries were collected in three coffee farms at different altitudes: Rosario Izapa (425 m), La Alianza (700 m) and Monteperla (950 m). An additional sample consisting of coffee berry borers reared in the laboratory on meridic diets was also included. Results show that there is a great diversity of fungi associated with this insect. 212 cultures, including 40 species distributed in 22 genera, were isolated. The recovery of fungi from the galleries was markedly less than from the borer's body. Three of the isolated species were undescribed; two belonging to the Penicillium and one to Hanseniaspora. Most of the species were collected from the cuticle of the insect, and the presence of fungi was not correlated with altitude. Fusarium, Penicillium, Candida and Aspergillus were the dominant genera with percentage abundance of 26.4, 18.7, 13.4 and 12.5%, respectively. The present study provides a detailed description of the mycobiota associated with H. hampei and represents a significant advance in the understanding of the relationship among this insect and the fungi associated with it.
authors.affiliation: El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto km 2.5, Tapachula, 30700 Chiapas, México.
authors.forename: Jeanneth
authors.initials: J
authors.lastname: Pérez
authors.forename: Francisco
authors.initials: F
authors.lastname: Infante
authors.forename: Fernando E
authors.initials: FE
authors.lastname: Vega
authors.forename: Francisco
authors.initials: F
authors.lastname: Holguín
authors.forename: Jorge
authors.initials: J
authors.lastname: Macías
authors.forename: Javier
authors.initials: J
authors.lastname: Valle
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Nieto
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
authors.forename: Cletus P
authors.initials: CP
authors.lastname: Kurtzman
authors.forename: Kerry
authors.initials: K
authors.lastname: O'Donnell
mesh_terms: D000818:Animals; D003069:Coffee / Q000469:parasitology*; D001517:Coleoptera / Q000382:microbiology*; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification*; D008800:Mexico; D010935:Plant Diseases / Q000469:parasitology*
chemical_list: D003069:Coffee