title: The crystal structure of the glutathione S-transferase-like domain of elongation factor 1Bgamma from Saccharomyces cerevisiae.
abstract: The crystal structure of the N-terminal 219 residues (domain 1) of the conserved eukaryotic translation elongation factor 1Bgamma (eEF1Bgamma), encoded by the TEF3 gene in Saccharomyces cerevisiae, has been determined at 3.0 A resolution by the single wavelength anomalous dispersion technique. The structure is overall very similar to the glutathione S-transferase proteins and contains a pocket with architecture highly homologous to what is observed in glutathione S-transferase enzymes. The TEF3-encoded form of eEF1Bgamma has no obvious catalytic residue. However, the second form of eEF1Bgamma encoded by the TEF4 gene contains serine 11, which may act catalytically. Based on the x-ray structure and gel filtration studies, we suggest that the yeast eEF1 complex is organized as an [eEF1A.eEF1Balpha.eEF1Bgamma]2 complex. A 23-residue sequence in the middle of eEF1Bgamma is essential for the stable dimerization of eEF1Bgamma and the quaternary structure of the eEF1 complex.
authors.affiliation: Department of Molecular Biology, University of Arhus, Gustav Wieds vej 10 C, 8000 Arhus C, Denmark.
authors.forename: Mads Gravers
authors.initials: MG
authors.lastname: Jeppesen
authors.forename: Pedro
authors.initials: P
authors.lastname: Ortiz
authors.forename: William
authors.initials: W
authors.lastname: Shepard
authors.forename: Terri Goss
authors.initials: TG
authors.lastname: Kinzy
authors.forename: Jens
authors.initials: J
authors.lastname: Nyborg
authors.forename: Gregers Rom
authors.initials: GR
authors.lastname: Andersen
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D005982:Glutathione Transferase / Q000737:chemistry; D015394:Molecular Structure; D020648:Peptide Elongation Factor 1 / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D020648:Peptide Elongation Factor 1; D029701:Saccharomyces cerevisiae Proteins; D005982:Glutathione Transferase