Interaction between acidic polysaccharides and proteins.
There was an ionic interaction between acidic polysaccharides (APS) and proteins at the pH range in which APS were negatively charged and proteins were positively charged, and in enzymes the interaction was detected as a change in the enzyme activity. At pH 4.7, acid phosphatase (pI, 5.4), alpha-glucosidase (pI, 5.7), and beta-glucosidase (pI, 7.3) were inhibited by APS to various extents. On the other hand, alpha-glucosidase and alkaline phosphatase (pI, 4.5) were not inhibited by APS at pH 6.8 and 9.8, respectively, most of these two enzymes being negatively charged at the respective pHs. Sulfated polysaccharides combined with hemoglobin (pI, 6.8 to approximately 7.0) by an ionic bond at pH 2 to make hemoglobin unsusceptible to proteolysis by pepsin, but polyuronides which were not charged at this pH did not affect hydrolysis of hemoglobin.
Tadera
Kenjiro
K
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan. tadera@chem.agri.kagoshima-u.ac.jp
Minami
Yuji
Y
Chohchi
Miki
M
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D006454:Hemoglobins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010434:Pepsin A / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D027861:Prunus / Q000201:enzymology; D013270:Stomach / Q000201:enzymology; D013552:Swine; D015003:Yeasts / Q000201:enzymology; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism
D004791:Enzyme Inhibitors; D004798:Enzymes; D065089:Glycoside Hydrolase Inhibitors; D006454:Hemoglobins; D011134:Polysaccharides; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D010434:Pepsin A