title: An electrochemical investigation of ligand-binding abilities of film-entrapped myoglobin.
abstract: Film-entrapped myoglobin exhibits well-defined electrochemistry which, upon ligand binding, displays a titratable redox potential shift. This effect has been observed to be highly dependent on the charged state of involved films. We have demonstrated that this approach may act as a model system for studies of molecular recognition between proteins and ligands.
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Yuting
authors.initials: Y
authors.lastname: Sun
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D008024:Ligands; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D009211:Myoglobin / Q000737:chemistry*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011485:Protein Binding; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D007093:Imidazoles; D008024:Ligands; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D009211:Myoglobin; D010713:Phosphatidylcholines; D012967:Sodium Dodecyl Sulfate; C029899:imidazole; D011094:Polyethyleneimine; D004247:DNA; D000077286:Cetrimonium