B cell deficiency confers protection from renal ischemia reperfusion injury.
Recent data have demonstrated a role for CD4(+) cells in the pathogenesis of renal ischemia reperfusion injury (IRI). Identifying engagement of adaptive immune cells in IRI suggests that the other major cell of the adaptive immune response, B cells, may also mediate renal IRI. An established model of renal IRI was used: 30 min of renal pedicle clamping was followed by reperfusion in B cell-deficient ( mu MT) and wild-type mice. Renal function was significantly improved in mu MT mice compared with wild-type mice at 24, 48, and 72 h postischemia. mu MT mice also had significantly reduced tubular injury. Both groups of mice had similar renal phagocyte infiltration postischemia assessed by myeloperoxidase levels and similar levels of CD4(+) T cell infiltration postischemia. Peritubular complement C3d staining was also similar in both groups. To identify the contribution of cellular vs soluble mechanism of action, serum transfer into mu MT mice partially restored ischemic phenotype, but B cell transfers did not. These data are the first demonstration of a pathogenic role for B cells in ischemic acute renal failure, with a serum factor as a potential underlying mechanism of action.
Burne-Taney
Melissa J
MJ
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Ascon
Dolores B
DB
Daniels
Frank
F
Racusen
Lorraine
L
Baldwin
William
W
Rabb
Hamid
H
D019264:Adoptive Transfer; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology* / Q000637:transplantation; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D015933:Complement C3d / Q000032:analysis; D007106:Immune Sera / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007275:Injections, Intravenous; D007668:Kidney / Q000098:blood supply* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D007677:Kidney Function Tests; D008231:Lymphopenia / Q000235:genetics* / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010586:Phagocytes / Q000473:pathology; D015427:Reperfusion Injury / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D013194:Staining and Labeling
D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015933:Complement C3d