Protein expressions in macrophage-derived foam cells: comparative analysis by two-dimensional gel electrophoresis.
OBJECTIVE
To study the overall protein expression changes induced by oxidized low-density lipoprotein (ox-LDL) in the U937 foam cells.


METHODS
Foam cell model was established by incubating the human monoblastic leukemia (U937) cells with ox-LDL. Each protein samples in U937 control cells and U937 foam cells were separated by two-dimensional gel electrophoresis (2-DE). After the gels were stained by silver staining method, the images were analyzed by PDquest 2D-image-analysis software (Bio-Rad). Some of the spots were available via the Internet with links to the U937 proteomic map provided from the ExPASy Proteomics server.


RESULTS
Using 2-DE, the overall protein map was obtained, in which 150 spots were matched with the control gel (match ratio: 75 %). Compared with U937 cells, 37 spots significantly changed in the foam cells (P<0.05), among which the expression levels in 28 spots increased and those in 9 spots decreased. Especially, 8 spots in U937 cells were absent in the foam cells, while 11 spots in the foam cells were absent in the control cells.


CONCLUSIONS
The changed protein profiles induced by ox-LDL in U937 foam cells were established to support the functional studies on the macrophage-derived foam cells in atherosclerotic pathological states.
Yu
Yan-Ling
YL
Department of Chemistry, Fudan University, Shanghai 200433, China.
Yang
Peng-Yuan
PY
Fan
Hui-Zhi
HZ
Huang
Zhen-Yu
ZY
Rui
Yao-Cheng
YC
Yang
Peng-Yuan
PY
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005487:Foam Cells / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D020298:U937 Cells
D008077:Lipoproteins, LDL; D011506:Proteins; C099252:oxidized low density lipoprotein