Method for the quantitative assay of fatty acid-bile acid conjugates by tandem mass spectrometry.
Fatty acid-bile acid conjugates and especially arachidyl amido cholic acid are synthetic molecules that were shown to prevent cholesterol gallstone formation in mice and hamsters as well as to dissolve pre-existing gallstones in mice. To measure these novel compounds we developed a liquid chromatography electrospray tandem mass spectrometry method based on the analysis of 100 microL of plasma with stearyl amido cholic acid (stamchol, 1.5 microM/L) added as internal standard. Repeatable calibrations between 0 and 50 microM/L exhibited consistent linearity and reproducibility. Inter- and intraassay C.V.s were 5.3-11.4% and 2.6-6.4%, respectively, at targeted concentrations of 0.1, 2.3 and 50 microM/L.
Goldiner
Ilana
I
Department of Gastroenterology, Tel Aviv Sourasky Medical Center, 64239 Tel Aviv, Israel.
Overmars
Henk
H
Groen
Albert K
AK
Kulik
Willem
W
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis / Q000737:chemistry*; D002138:Calibration; D006224:Cricetinae; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D012015:Reference Standards; D015203:Reproducibility of Results
D001647:Bile Acids and Salts; D005227:Fatty Acids