Inhibitory effects of DA-9601 on ethanol-induced gastrohemorrhagic lesions and gastric xanthine oxidase activity in rats.
The exposure of gastric mucosa to ethanol produces pathological changes such as inflammatory process, hemorrhagic erosions, even acute ulcers. The gastric mucosal lesions accompanied by a significant decrease of gastric blood flow and increase of reactive oxygen species (ROS) implicate a role of xanthine oxidase in ethanol-induced gastric hemorrhagic erosions. DA-9601, a novel antipeptic formulation of extracts of Artemisia asiatica Nakai, was studied for its inhibitory effect on gastric xanthine oxidase activity and type conversion of the enzyme that has a profound role in free radical generation. Intubation of absolute ethanol (4 g/kg) significantly induced gastrohemorrhagic lesions and lipid peroxidation in the rat stomach. Oral administration of DA-9601 at 40 mg/kg body weight significantly reduced ethanol-induced gastric mucosal hemorrhagic lesions and lipid peroxidation, which was proportional to the inhibitory effect of DA-9601 on alcohol-induced xanthine oxidase-type conversion and enzyme activity. The results suggest that alcohol-induced gastric mucosal damage may be, in part, due to the increased activity of xanthine oxidase and type conversion rate of the enzyme and that the preventive effect of DA-9601 on gastrohemorrhagic lesions would result from its inhibitory action against xanthine oxidase and oxidative stress in alcohol-treated rats.
Huh
Keun
K
College of Pharmacy, Yeungnam University, Gyongsan 712-749, South Korea.
Kwon
Tae Hyup
TH
Shin
Uk Sup
US
Kim
Won Bae
WB
Ahn
Byoung Ok
BO
Oh
Tae Young
TY
Kim
Jung-Ae
JA
D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D019306:Artemisia / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000627:therapeutic use*; D000431:Ethanol / Q000633:toxicity*; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
D000897:Anti-Ulcer Agents; C116959:DA 9601; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000431:Ethanol; D014969:Xanthine Oxidase