title: Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression.
abstract: We have studied the genomic organization and transcription of the histone H2A genes in the protozoan parasite Leishmania infantum. In the parasite genome 2 gene clusters exist, each containing 3 H2A gene copies. Sequence analyses showed the existence of significant sequence divergence among the H2A genes, mainly in their 5'- and 3'-untranslated regions (UTRs). Also, the existence of allelic alternatives has been evidenced. Based on the divergence in the 3'UTR regions, we have defined 3 classes of H2A transcripts, which are present at different levels in L. infantum promastigotes. However, transcription of the 3 classes of H2A genes occurs at similar levels, as measured by nuclear run-on assays, indicating that their abundance is regulated post-transcriptionally. Also, differences in regulation were observed among the H2A transcripts: the levels of transcripts with 3'-UTR type I and type III are affected by growth phase whereas transcripts with 3'-UTR type II, that are barely detected, remain constant. It is likely that the complexity, in both gene organization and differential expression exhibited by the L. infantum H2A genes, is imposed by the nature of the post-transcriptional mechanisms of regulation operating in this parasite.
authors.affiliation: Centro de Biologia Molecular 'Severo Ochoa', Universidad Autónoma de Madrid, Madrid, E-28049 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Soto
authors.forename: L
authors.initials: L
authors.lastname: Quijada
authors.forename: R
authors.initials: R
authors.lastname: Larreta
authors.forename: S
authors.initials: S
authors.lastname: Iborra
authors.forename: C
authors.initials: C
authors.lastname: Alonso
authors.forename: J M
authors.initials: JM
authors.lastname: Requena
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation; D017125:Genes, Protozoan / Q000235:genetics*; D018503:Genome, Protozoan; D006657:Histones / Q000235:genetics*; D018314:Leishmania infantum / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D012333:RNA, Messenger