title: Protein kinase C/zeta (PRKCZ) gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes.
abstract: OBJECTIVE
  To identify the susceptible gene (s) for type 2 diabetes in the previously mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.
  
  
  METHODS
  Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed.
  
  
  RESULTS
  Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/zetagene (PRKCZ) was statistically different between the case and control groups(P<0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.
  
  
  CONCLUSIONS
  PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Sun
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Wu
authors.forename: Wei-Nan
authors.initials: WN
authors.lastname: Du
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Bo-Qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yao
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Mo-Miao
authors.initials: MM
authors.lastname: Xiong
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.forename: Fu-De
authors.initials: FD
authors.lastname: Fang
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics*; D000099221:Protein Kinase C zeta
chemical_list: D011493:Protein Kinase C; D000099221:Protein Kinase C zeta