The tumor promoting effect of lime-piper betel quid in JB6 cells.
Betel quid chewing is a general behavior in Taiwan, India, southeastern Asian and South Africa. In this study, microculture tetrazolium test (MTT) showed that the extract of lime-piper betel quid (LPB) (1.0-20 mg/ml) was toxic to JB6 cells. Cells exposed of LPB (0.1, 0.5, 1.0 mg/ml) for 7 days resulted in changes in cytomorphology with characteristics of carcinogenesis. With a long-term treatment (approximately 30 days) of low doses of LPB (1, 5, 10 microg/ml), the production of H2O2 and the activity of myeloperoxidase (MPO) and ornithine decarboxylase (ODC) were increased in JB6 cells. Cell cycle analysis showed a decrease in the G1 phase and an accumulation in the S phase 48 h after LPB treatment. When treating with 0.5 mg/ml LPB for 15 days as a promoter, type III foci were formed in the JB6 culture. These results demonstrated the tumor promotional effect of LPB in JB6 cells.
Lin
Ming-Hsun
MH
Institute of Biochemistry, College of Medicine, Chung Shan Medical University, No 110, Sec 1, Chien Kuo North Road, Taichung, 402 Taiwan.
Chou
Fen-Pi
FP
Huang
Hui-Pei
HP
Hsu
Jeng-Dong
JD
Chou
Ming-Yung
MY
Wang
Chau-Jong
CJ
D000818:Animals; D001114:Areca / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D032082:Citrus aurantiifolia / Q000633:toxicity*; D016193:G1 Phase / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010087:Oxides / Q000633:toxicity*; D009195:Peroxidase / Q000378:metabolism; D031701:Piper / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D016196:S Phase / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles
D017610:Calcium Compounds; D002273:Carcinogens; D010087:Oxides; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C495400:lime-piper betel quid; D006861:Hydrogen Peroxide; D009195:Peroxidase; D009955:Ornithine Decarboxylase; C022616:thiazolyl blue