Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method.
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10  to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
Rubiano
Claudia C
CC
Laboratorio Bioquímica, Instituto Nacional de Salud, Bogotá, Colombia.
Wasserman
Moises
M
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D010963:Plasmodium falciparum / Q000201:enzymology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics*
D019098:Telomerase