Migration, proliferation, and invasion of human glioma cells following treatment with simvastatin.
This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines, GaMg and U-87 Mg, grown as multicellular tumour spheroids after 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase inhibitor (simvastatin) therapy. Migration and proliferation studies were performed using simvastatin in concentrations of 0.2-30 microg/ml(-1). A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. A dose-dependent growth and migratory inhibitory response to simvastatin treatment was observed. Marked invasion of the glioma spheroids into the brain aggregates could be seen in both treated and nontreated groups. Simvastatin therapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.
Gliemroth
Jan
J
Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany. jan.gliemroth@t-online.de
Zulewski
Henryk
H
Arnold
Hans
H
Terzis
A Jorge A
AJ
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000503:physiopathology; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology*; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018874:Spheroids, Cellular / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019821:Simvastatin