title: Comparing protein abundance and mRNA expression levels on a genomic scale.
abstract: Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA. Kenneth.Williams@yale.edu
authors.forename: Dov
authors.initials: D
authors.lastname: Greenbaum
authors.forename: Christopher
authors.initials: C
authors.lastname: Colangelo
authors.forename: Kenneth
authors.initials: K
authors.lastname: Williams
authors.forename: Mark
authors.initials: M
authors.lastname: Gerstein
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D023281:Genomics / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D011506:Proteins; D012333:RNA, Messenger