title: Genotoxic effects of occupational exposure to lead and cadmium.
abstract: This study was designed to assess genotoxic damage in somatic cells of workers in a Polish battery plant after high-level occupational exposure to lead (Pb) and cadmium (Cd), by use of the following techniques: the micronucleus (MN) assay, combined with in situ fluorescence hybridization (FISH) with pan-centromeric probes, analysis of sister chromatid exchanges (SCEs), and the comet assay. Blood samples from 44 workers exposed to lead, 22 exposed to cadmium, and 52 unexposed persons were used for SCE and MN analysis with 5'-bromodeoxyuridine (BrdU) or cytokinesis block, respectively. In parallel, the comet assay was performed with blood samples from the same persons for detection of DNA damage, including single-strand breaks (SSB) and alkali-labile sites (ALS). In workers exposed mostly to lead, blood Pb concentrations ranged from 282 to 655 microg/l, while the range in the controls was from 17 to 180 microg/l. Cd concentration in lead-exposed workers fell in the same range as for the controls. In workers exposed mainly to cadmium, blood Cd levels varied from 5.4 to 30.8 microg/l, with respective values for controls within the range of 0.2-5.7 microg/l. Pb concentrations were similar as for the controls. The incidence of MN in peripheral lymphocytes from workers exposed to Pb and Cd was over twice as high as in the controls (P<0.01). Using a combination of conventional scoring of MN and FISH with pan-centromeric probes, we assessed that this increase may have been due to clastogenic as well as aneugenic effects. In Cd- and Pb-exposed workers, the frequency of SCEs as well as the incidence of leukocytes with DNA fragmentation in lymphocytes were slightly, but significantly increased ( P<0.05) as compared with controls. After a 3h incubation of the cells to allow for DNA repair, a clear decrease was found in the level of DNA damage in the controls as well as in the exposed workers. No significant influence of smoking on genotoxic damage could be detected in metal-exposed cohorts. Our findings indicate that lead and cadmium induce clastogenic as well as aneugenic effects in peripheral lymphocytes, indicating a potential health risk for working populations with significant exposures to these heavy metals.
authors.affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland. jadwigap@imp.lodz.pl
authors.forename: J
authors.initials: J
authors.lastname: Palus
authors.forename: K
authors.initials: K
authors.lastname: Rydzynski
authors.forename: E
authors.initials: E
authors.lastname: Dziubaltowska
authors.forename: K
authors.initials: K
authors.lastname: Wyszynska
authors.forename: A T
authors.initials: AT
authors.lastname: Natarajan
authors.forename: R
authors.initials: R
authors.lastname: Nilsson
mesh_terms: D000328:Adult; D024422:Aneugens / Q000009:adverse effects; D002104:Cadmium / Q000009:adverse effects* / Q000097:blood; D016022:Case-Control Studies; D020552:Comet Assay; D004247:DNA / Q000032:analysis / Q000187:drug effects; D004249:DNA Damage; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D007854:Lead / Q000009:adverse effects* / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009152:Mutagenicity Tests; D009153:Mutagens / Q000009:adverse effects; D016273:Occupational Exposure / Q000009:adverse effects*; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D024422:Aneugens; D009153:Mutagens; D002104:Cadmium; D007854:Lead; D004247:DNA