Sequence and expression of a cDNA encoding both pituitary adenylate cyclase activating polypeptide and growth hormone-releasing hormone in grouper (Epinephelus coioides).
Both pituitary adenylate cyclase activating polypeptide  PACAP   and growth hormone-releasing hormone   GHRH   belong to the vasoactive intestinal polypeptide- glucagon-secretin family. They are encoded on the same gene in vertebrates (birds, amphibian, fish). Although the gene encoding PACAP and GHRH has been cloned in other fish species, characterization of this gene in the commercially important grouper (Epinephelus coioides) has not been previously reported. In this study, the GHRH/PACAP cDNA was cloned from grouper hypothalamic tissue. Two cDNA variants of the GHRH/PACAP precursor gene were identified as a result of alternative splicing, a long form encoding both PACAP and GHRH and a short form encoding only PACAP. Both the long and the short forms of the GHRH/PACAP precursor cDNA were identified in grouper 22 issues as well as expression in embryos and larvae by semi-quantitative RT-PCR detection. PACAP / GHRH precursor mRNA was expressed at a high level in the central nervous system than in several peripheral tissues. The data presented here provide the report of PACAP/GHRH mRNA expression in eye and gill tissues in fish. PACAP/GHRH mRNA was expressed in grouper embryo and all larval stages examined and was expressed at a high level starting on neurula stage onwards. The result suggests that PACAP/GHRH had an important role in the development of embryos and larvae, especially in neurula appearance stage.
Jiang
Yong
Y
Institute of Aquatic Economical Animal Guangdong Provincial Key Laboratory for Aquatic Economical Animal, Zhongshan University, Guangzhou 510275, China.
Li
Wen-Sheng
WS
Xie
Jun
J
Lin
Hao-Ran
HR
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D020033:Protein Isoforms / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D020033:Protein Isoforms; D011498:Protein Precursors; D012333:RNA, Messenger; D013007:Growth Hormone-Releasing Hormone