Functions of the ectodomain and cytoplasmic tyrosine phosphatase domains of receptor protein tyrosine phosphatase Dlar in vivo.
The receptor protein tyrosine phosphatase (PTPase) Dlar has an ectodomain consisting of three immunoglobulin (Ig)-like domains and nine fibronectin type III (FnIII) repeats and a cytoplasmic domain consisting of two PTPase domains, membrane-proximal PTP-D1 and C-terminal PTP-D2. A series of mutant Dlar transgenes were introduced into the Drosophila genome via P-element transformation and were then assayed for their capacity to rescue phenotypes caused by homozygous loss-of-function genotypes. The Ig-like domains, but not the FnIII domains, are essential for survival. Conversely, the FnIII domains, but not the Ig-like domains, are required during oogenesis, suggesting that different domains of the Dlar ectodomain are involved in distinct functions during Drosophila development. All detectable PTPase activity maps to PTP-D1 in vitro. The catalytically inactive mutants of Dlar were able to rescue Dlar(-/-) lethality nearly as efficiently as wild-type Dlar transgenes, while this ability was impaired in the PTP-D2 deletion mutants DlarDeltaPTP-D2 and Dlar(bypass). Dlar-C1929S, in which PTP-D2 has been inactivated, increases the frequency of bypass phenotype observed in Dlar(-/-) genotypes, but only if PTP-D1 is catalytically active in the transgene. These results indicate multiple roles for PTP-D2, perhaps by acting as a docking domain for downstream elements and as a regulator of PTP-D1.
Krueger
Neil X
NX
Dana-Farber Cancer Institute/Harvard Cancer Center, Harvard Medical School, Boston, Massachusetts 02115, USA.
Reddy
R Sreekantha
RS
Johnson
Karl
K
Bateman
Jack
J
Kaufmann
Nancy
N
Scalice
Daniella
D
Van Vactor
David
D
Saito
Haruo
H
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins; D005260:Female; D017353:Gene Deletion; D015870:Gene Expression; D005982:Glutathione Transferase / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009866:Oogenesis; D017354:Point Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D054557:Receptor-Like Protein Tyrosine Phosphatases; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D019076:Transgenes
D029721:Drosophila Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D005982:Glutathione Transferase; C117978:Lar protein, Drosophila; D017027:Protein Tyrosine Phosphatases; D054557:Receptor-Like Protein Tyrosine Phosphatases