title: Metabolic model for acetate uptake by a mixed culture of phosphate- and glycogen-accumulating organisms under anaerobic conditions.
abstract: This paper proposes a new metabolic model for acetate uptake by a mixed culture of phosphate- and glycogen-accumulating organisms (PAOs and GAOs) under anaerobic conditions. The model uses variable overall stoichiometry based on the assumption that PAOs may have the ability of using the glyoxylate pathway to produce the required reducing power for polyhydroxyalkonate (PHA) synthesis. The proposed model was tested and verified by experimental results. A sequencing batch reactor system was operated for enhanced biological phosphorus removal (EBPR) with acetate as the sole carbon source at different influent acetate/phosphate ratios. The resulting experimental data supported the validity of the proposed model, indicating the presence of GAOs for all tested HAc/P ratios, especially under P-limiting conditions. Strong agreement is observed between experimental values and model predictions for all model components, namely, PHB production, PHA composition, glycogen utilization, and P release.
authors.affiliation: Istanbul Technical University, ITU Insaat Fakultesi Cevre Muhendisligi Bolumu, Istanbul, Turkey. nartan@ins.its.edu.tr
authors.forename: Nevin
authors.initials: N
authors.lastname: Yagci
authors.forename: Nazik
authors.initials: N
authors.lastname: Artan
authors.forename: Emine Ubay
authors.initials: EU
authors.lastname: Cokgör
authors.forename: Clifford W
authors.initials: CW
authors.lastname: Randall
authors.forename: Derin
authors.initials: D
authors.lastname: Orhon
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D018920:Coculture Techniques / Q000379:methods*; D003198:Computer Simulation; D004734:Energy Metabolism / Q000502:physiology*; D006003:Glycogen / Q000493:pharmacokinetics*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism; D010710:Phosphates / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012722:Sewage / Q000382:microbiology*
chemical_list: D000085:Acetates; D009097:Multienzyme Complexes; D010710:Phosphates; D012722:Sewage; D006003:Glycogen