title: The inducible CXCR3 ligands control plasmacytoid dendritic cell responsiveness to the constitutive chemokine stromal cell-derived factor 1 (SDF-1)/CXCL12.
abstract: The recruitment of selected dendritic cell (DC) subtypes conditions the class of the immune response. Here we show that the migration of human plasmacytoid DCs (pDCs), the blood natural interferon alpha-producing cells, is induced upon the collective action of inducible and constitutive chemokines. Despite expression of very high levels of CXCR3, pDCs do not respond efficiently to CXCR3 ligands. However, they migrate in response to the constitutive chemokine stromal cell-derived factor 1 (SDF-1)/CXCL12 and CXCR3 ligands synergize with SDF-1/CXCL12 to induce pDC migration. This synergy reflects a sensitizing effect of CXCR3 ligands, which, independently of a gradient and chemoattraction, decrease by 20-50-fold the threshold of sensitivity to SDF-1/CXCL12. Thus, the ability of the constitutive chemokine SDF-1/CXCL12 to induce pDC recruitment might be controlled by CXCR3 ligands released during inflammation such as in virus infection. SDF-1/CXCL12 and the CXCR3 ligands Mig/CXCL9 and ITAC/CXCL1 display adjacent expression both in secondary lymphoid organs and in inflamed epithelium from virus-induced pathologic lesions. Because pDCs express both the lymph node homing molecule l-selectin and the cutaneous homing molecule cutaneous lymphocyte antigen, the cooperation between inducible CXCR3 ligands and constitutive SDF-1/CXCL12 may regulate recruitment of pDCs either in lymph nodes or at peripheral sites of inflammation.
authors.affiliation: Laboratory for Immunological Research, Schering-Plough, 27 chemin des Peupliers, BP 11, 69571 Dardilly, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Vanbervliet
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bendriss-Vermare
authors.forename: Catherine
authors.initials: C
authors.lastname: Massacrier
authors.forename: Bernhard
authors.initials: B
authors.lastname: Homey
authors.forename: Odette
authors.initials: O
authors.lastname: de Bouteiller
authors.forename: Francine
authors.initials: F
authors.lastname: Brière
authors.forename: Giorgio
authors.initials: G
authors.lastname: Trinchieri
authors.forename: Christophe
authors.initials: C
authors.lastname: Caux
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D039521:CD11c Antigen / Q000276:immunology; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000276:immunology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000097:blood; D008024:Ligands; D008213:Lymphocyte Activation / Q000276:immunology; D010950:Plasma Cells / Q000187:drug effects / Q000276:immunology; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000502:physiology*; D017154:Stromal Cells / Q000276:immunology
chemical_list: D015703:Antigens, CD; D039521:CD11c Antigen; C515255:CXCL12 protein, human; C515187:CXCR3 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016898:Interferon-alpha; D008024:Ligands; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine