title: 4-Hydroxycoumarin disorganizes the actin cytoskeleton in B16-F10 melanoma cells but not in B82 fibroblasts, decreasing their adhesion to extracellular matrix proteins and motility.
abstract: This study determined the in vitro effects of 4-hydroxycoumarin (4-HC) employing the melanoma cell line B16-F10 and the non-malignant fibroblastic cell line B82. 4-HC disorganized the actin cytoskeleton in B16-F10 cells, but not in B82 fibroblasts. Cytoskeletal disorganization correlated with reductions in cell adhesion to four extracellular matrix proteins and inhibition of random motility. 4-HC did not modify cell viability or actin expression, but decreased tyrosine phosphorylation of several proteins in melanoma cells. Because adhesion of tumor cells to extracellular matrix is required during the metastatic process, 4-HC might be useful as an adjuvant therapy for melanoma.
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Apdo. Postal 70-297, Ciudad Universitaria, México, DF 04510, Mexico. marcovelasco@correo.unam.mx
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Velasco-Velázquez
authors.forename: José
authors.initials: J
authors.lastname: Agramonte-Hevia
authors.forename: Diana
authors.initials: D
authors.lastname: Barrera
authors.forename: Alejandro
authors.initials: A
authors.lastname: Jiménez-Orozco
authors.forename: María Juana
authors.initials: MJ
authors.lastname: García-Mondragón
authors.forename: Nicandro
authors.initials: N
authors.lastname: Mendoza-Patiño
authors.forename: Abraham
authors.initials: A
authors.lastname: Landa
authors.forename: Juan
authors.initials: J
authors.lastname: Mandoki
mesh_terms: D015110:4-Hydroxycoumarins / Q000494:pharmacology*; D000199:Actins / Q000187:drug effects*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008546:Melanoma, Experimental / Q000503:physiopathology; D051379:Mice; D009363:Neoplasm Proteins / Q000187:drug effects / Q000378:metabolism; D019000:Phosphotyrosine / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D015110:4-Hydroxycoumarins; D000199:Actins; D016326:Extracellular Matrix Proteins; D009363:Neoplasm Proteins; D019000:Phosphotyrosine; C068805:4-hydroxycoumarin