title: Citrus psorosis virus RNA 1 is of negative polarity and potentially encodes in its complementary strand a 24K protein of unknown function and 280K putative RNA dependent RNA polymerase.
abstract: Citrus psorosis virus (CPsV), the type member of genus Ophiovirus, has three genomic RNAs. Complete sequencing of CPsV RNA 1 revealed a size of 8184 nucleotides and Northern blot hybridization with chain specific probes showed that its non-coding strand is preferentially encapsidated. The complementary strand of RNA 1 contains two open reading frames (ORFs) separated by a 109-nt intergenic region, one located near the 5'-end potentially encoding a 24K protein of unknown function, and another of 280K containing the core polymerase motifs characteristic of viral RNA-dependent RNA polymerases (RdRp). Comparison of the core RdRp motifs of negative-stranded RNA viruses, supports grouping CPsV, Ranunculus white mottle virus (RWMV) and Mirafiori lettuce virus (MiLV) within the same genus (Ophiovirus), constituting a monophyletic group separated from all other negative-stranded RNA viruses. Furthermore, RNAs 1 of MiLV, CPsV and RWMV are similar in size and those of MiLV and CPsV also in genomic organization and sequence.
authors.affiliation: Facultad de Ciencias Exactas, Instituto de Bioquímica y Biología Molecular, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Naum-Onganía
authors.forename: Selma
authors.initials: S
authors.lastname: Gago-Zachert
authors.forename: Eduardo
authors.initials: E
authors.lastname: Peña
authors.forename: Oscar
authors.initials: O
authors.lastname: Grau
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Garcia
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002957:Citrus / Q000821:virology*; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000737:chemistry / Q000235:genetics; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*
chemical_list: D012324:RNA-Dependent RNA Polymerase