title: Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex.
abstract: The mammalian ATM/PI 3-kinase-related TRRAP protein was previously found to be a component of a multi-protein histone acetyltransferase (HAT) complex containing the HAT TIP60. In this report, we identify a previously uncharacterized protein encoded by the FLJ10914 ORF, which we designate MRGBP, as a new component of the TRRAP/TIP60 HAT complex. In addition, through purification of MRGBP and its associated proteins from HeLa cell nuclear extracts, we identify the thyroid receptor coactivating protein (TRCp120), DMAP1, and the related MRG15 and MRGX proteins as MRGBP-associating proteins, and we present biochemical evidence that they are previously unrecognized components of the TRRAP/TIP60 HAT complex. Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.forename: Jingji
authors.initials: J
authors.lastname: Jin
authors.forename: Chieri
authors.initials: C
authors.lastname: Tomomori-Sato
authors.forename: Shigeo
authors.initials: S
authors.lastname: Sato
authors.forename: Irina
authors.initials: I
authors.lastname: Sorokina
authors.forename: Tari J
authors.initials: TJ
authors.lastname: Parmely
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Conaway
authors.forename: Joan Weliky
authors.initials: JW
authors.lastname: Conaway
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry* / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D000074443:Lysine Acetyltransferase 5; D013058:Mass Spectrometry; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D016366:Open Reading Frames; D011485:Protein Binding; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C411744:DMAP1 protein, human; C470833:MORF4L1 protein, human; C479739:MRGBP protein, human; D009687:Nuclear Proteins; D011988:Receptors, Thyroid Hormone; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C113829:transformation-transcription domain-associated protein; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; C098489:KAT5 protein, human; D000074443:Lysine Acetyltransferase 5