[Effects of naloxone on rat cortical neuron apoptosis induced by anoxia].
OBJECTIVE
To investigate the damage of anoxia on the cultured rat's cortical neurons and the protective effects of naloxone.


METHODS
Cortical neurons cultured for 12 days were randomly divided into three groups: control, anoxic group and anoxic group plus naloxone treated. Cortical neurons were exposed to anoxic environment for 6 hours and then cultured for 24 hours under normoxic condition. The apoptosis of neurons was detected with the method of TdT-mediated dUTP nick end labeling (TUNEL) and the apoptotic rate of neurons was measured using flow cytometry.


RESULTS
Anoxia could lead to increasing apoptosis and death of rate cortical neurons. Naloxone could reduce neuron apoptosis. There was a significant difference between the anoxic group and anoxic group plus naloxone (P<0.01).


CONCLUSIONS
Naloxone can ameliorate the toxicity damage of neurons caused by anoxia through reducing neuron apoptosis, which suggests that naloxone could protect rat's cortical neurons from damage under anoxia condition.
Song
Yang
Y
Department of Emergency, General Hospital of People's Liberation Army, Beijing 100853, China.
Shen
Hong
H
Ding
Ai-shi
AS
Fan
Ming
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology; D020287:In Situ Nick-End Labeling; D009270:Naloxone / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D018696:Neuroprotective Agents; D009270:Naloxone