title: Potassium channels in human umbilical artery cells.
abstract: OBJECTIVE
  To identify K+ channels of smooth muscle of human umbilical artery using the patch-clamp technique and to study their effect on resting tone of umbilical artery rings.
  
  
  METHODS
  Whole-cell and single-channel patch-clamp recordings in enzymatically isolated smooth muscle cells were made. Measurements of developed isometric force were performed on intact tissue.
  
  
  RESULTS
  Delayed rectifier K+ channels (KDR) and large-conductance Ca2+-activated K+ channels (BKCa) contribute to the whole-cell voltage- and time-dependent outward K+ current, as it was specifically inhibited by 5 mM 4-aminopyridine (4-AP; KDR blocker) (92 +/- 4% at 0 mV, n = 7), by 1 mM tetraethylammonium (TEA; BKCa blocker) (71 +/- 4% at +60 mV, n = 4), and by 200 nM iberiotoxin (BKCa blocker) (64 +/- 7% at +60 mV, n = 4). In outside-out patches, BKCa channels had a single-channel conductance of 132 +/- 4 pS (n = 24) in asymmetric K+ conditions and 216 +/- 4 pS (n = 4) in a symmetric K+ gradient. The activity of the BKCa channels was significantly augmented by 1 microM Ca2+ in the inside-out configuration. 4-AP had no effect on resting tone of intact arterial rings. TEA produced contraction of arterial rings whereas phloretin, an activator of BKCa, relaxed them, which means that BKCa channels are functional in intact tissue and are involved in the maintenance of resting tone in this human vessel.
  
  
  CONCLUSIONS
  The identities of K+ channels in the human umbilical artery were shown using the patch-clamp technique, and the physiologic effect of K+ channels on resting tone was documented.
authors.affiliation: Anatomía y Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. veronica@nahuel.biol.unlp.edu.ar
authors.forename: Verónica
authors.initials: V
authors.lastname: Milesi
authors.forename: Jesica
authors.initials: J
authors.lastname: Raingo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rebolledo
authors.forename: Angela O
authors.initials: AO
authors.lastname: Grassi de Gende
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004553:Electric Conductivity; D005260:Female; D006801:Humans; D008564:Membrane Potentials; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000032:analysis* / Q000502:physiology; D011247:Pregnancy; D019789:Tetraethylammonium / Q000494:pharmacology; D014469:Umbilical Arteries / Q000737:chemistry*
chemical_list: D010455:Peptides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D019789:Tetraethylammonium; C064719:iberiotoxin; D015761:4-Aminopyridine; D002118:Calcium