title: Variants of the CD40 ligand gene are not associated with increased susceptibility to tuberculosis in West Africa.
abstract: Evidence for linkage between tuberculosis and human chromosomal region Xq26 has previously been described. The costimulatory molecule CD40 ligand, encoded by TNFSF5 and located at Xq26.3, is a promising positional candidate. Interactions between CD40 ligand and CD40 are involved in the development of humoral- and cell-mediated immunity, as well as the activation of macrophages, which are the primary host and effector cells for Mycobacterium tuberculosis. We hypothesised that common variation within TNFSF5 might affect susceptibility to tuberculosis disease and, thus, might be responsible for the observed linkage to Xq26. Sequencing 32 chromosomes from a Gambian population identified nine common polymorphisms within the coding, 3' and 5' regulatory sequences of the gene. Six single nucleotide polymorphisms (SNPs) and a 3' microsatellite were genotyped in 121 tuberculosis patients and their available parents. No association with tuberculosis was detected for these variants using a transmission disequilibrium test, although one SNP at -726 showed some evidence of association in males. This finding, however, did not replicate in a separate case control study of over 1,200 West African individuals. We conclude that common genetic variation in TNFSF5 is not likely to affect tuberculosis susceptibility in West Africa and the linkage observed in this region is not due to variation in TNFSF5.
authors.affiliation: The Henry Wellcome Building of Genomic Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, UK.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Campbell
authors.forename: Pardis
authors.initials: P
authors.lastname: Sabeti
authors.forename: Katherine
authors.initials: K
authors.lastname: Fielding
authors.forename: Jackson
authors.initials: J
authors.lastname: Sillah
authors.forename: Boubacar
authors.initials: B
authors.lastname: Bah
authors.forename: Per
authors.initials: P
authors.lastname: Gustafson
authors.forename: Kebba
authors.initials: K
authors.lastname: Manneh
authors.forename: Ida
authors.initials: I
authors.lastname: Lisse
authors.forename: Giorgio
authors.initials: G
authors.lastname: Sirugo
authors.forename: Richard
authors.initials: R
authors.lastname: Bellamy
authors.forename: Steve
authors.initials: S
authors.lastname: Bennett
authors.forename: Peter
authors.initials: P
authors.lastname: Aaby
authors.forename: Keith P W J
authors.initials: KP
authors.lastname: McAdam
authors.forename: Oumou
authors.initials: O
authors.lastname: Bah-Sow
authors.forename: Christian
authors.initials: C
authors.lastname: Lienhardt
authors.forename: Adrian V S
authors.initials: AV
authors.lastname: Hill
mesh_terms: D000354:Africa, Western / Q000453:epidemiology; D023201:CD40 Ligand / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D014376:Tuberculosis / Q000235:genetics*
chemical_list: D023201:CD40 Ligand