Effect of quercetin on hypoxic injury in freshly isolated rat proximal tubules.
The bioflavonoid quercetin, which has antioxidant properties, protects renal tubular epithelial cells from oxidant-induced injury by inhibiting lipid peroxidation. We examined the effect of quercetin on hypoxia-induced injury in freshly isolated rat renal proximal tubules. Hypoxia induced rapid loss of cellular ATP, followed by functional and structural alterations measured as a decrease in tubular potassium content and sequentially by an increase in lactate dehydrogenase release. Furthermore, hypoxia increased lipid peroxidation, measured as thiobarbituric acid-reactive substances. Quercetin significantly inhibited hypoxia-induced functional and structural tubular injury in addition to lipid peroxidation but did not alter hypoxia-induced ATP depletion. These results demonstrate the potency of the bioflavonoid quercetin in protecting proximal tubules from hypoxic injury, which is independent of tubular energy metabolism and may be related to the inhibition of lipid peroxidation.
Pietruck
Frank
F
Klinik für Nieren- und Hochdrukkrankheiten, Universitätsklinikum, 45122, Essen, Germany.
Kuhlmann
Martin K
MK
Lange
Bettina
B
Feldkamp
Thorsten
T
Herget-Rosenthal
Stefan
S
Rauen
Ursula
U
Burkhardt
Gunther
G
Kohler
Hans
H
Philipp
Thomas
T
Kribben
Andreas
A
D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000860:Hypoxia / Q000188:drug therapy*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D011188:Potassium