Maximum-entropy decomposition of fluorescence correlation spectroscopy data: application to liposome-human serum albumin association.
Fluorescence correlation spectroscopy was used to measure the diffusion behavior of a mixture of DMPC or DMPC/DMPG liposomes with human serum albumin (HSA) and mesoporphyrin (MP), which was used as the fluorescent label for liposomes and HSA as well. For decomposing the fluorescence intensity autocorrelation function (ACF) into components corresponding to a liposome population, HSA and MP, we used a maximum entropy procedure that computes a distribution of diffusion times consistent with the ACF data. We found that a simple parametric non-linear fit with a discrete set of decay components did not converge to a stable parameter set. The distribution calculated with the maximum entropy method was stable and the average size of the particles calculated from the effective diffusion time was in good agreement with the data determined using the discrete-component fit.
Módos
Károly
K
Division of Biophysics of Macromolecules, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Galántai
Rita
R
Bárdos-Nagy
Irén
I
Wachsmuth
Malte
M
Tóth
Katalin
K
Fidy
Judit
J
Langowski
Jörg
J
D055592:Biophysical Phenomena; D001703:Biophysics; D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D019277:Entropy; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry*; D008652:Mesoporphyrins; D010715:Phosphatidylglycerols / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000706:statistics & numerical data
D005456:Fluorescent Dyes; D008081:Liposomes; D008652:Mesoporphyrins; D010715:Phosphatidylglycerols; D012709:Serum Albumin; C031468:mesoporphyrin IX; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine