Transcription through the roadblocks: the role of RNA polymerase cooperation.
During transcription, cellular RNA polymerases (RNAP) have to deal with numerous potential roadblocks imposed by various DNA binding proteins. Many such proteins partially or completely interrupt a single round of RNA chain elongation in vitro. Here we demonstrate that Escherichia coli RNAP can effectively read through the site-specific DNA-binding proteins in vitro and in vivo if more than one RNAP molecule is allowed to initiate from the same promoter. The anti-roadblock activity of the trailing RNAP does not require transcript cleavage activity but relies on forward translocation of roadblocked complexes. These results support a cooperation model of transcription whereby RNAP molecules behave as 'partners' helping one another to traverse intrinsic and extrinsic obstacles.
Epshtein
Vitaly
V
Department of Biochemistry, New York University Medical Center, New York, NY 10016, USA.
Toulmé
Francine
F
Rahmouni
A Rachid
AR
Borukhov
Sergei
S
Nudler
Evgeny
E
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006706:Homeostasis; D007763:Lac Operon; D008954:Models, Biological; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000235:genetics*
D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases