Procedure for the quantification of the biomarker (2-methoxyethoxy)acetic acid in human urine samples.
An accurate and precise procedure was developed for the detection and quantification of (2-methoxyethoxy)acetic acid (MEAA), a metabolite and biomarker for human exposure to 2-(2-methoxyethoxy)ethanol. The compound 2-(2-methoxyethoxy)ethanol has a wide array of industrial applications including its use as an additive in military jet fuel. Exposure to 2-(2-methoxyethoxy)ethanol is a health concern owing to its toxicity which includes developmental and teratogenic properties. Sample preparation consisted of liquid-liquid extraction (LLE) and esterification of MEAA to produce the ethyl ester. Measurement was by a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a HP-1 capillary column. Recovery studies of spiked blank urine demonstrated good accuracy and precision; recovery varied between 95 and 103% with relative standard deviations of 8.6% and less. The limit of detection (LOD) for this procedure was found to range from 0.02 to 0.08 microg/ml equivalent levels of MEAA in urine. These data and other aspects of the validation of this procedure will be discussed.
B'Hymer
C
C
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Cincinnati, OH 45226, USA. cbhymer@cdc.org
Cheever
K L
KL
Butler
M A
MA
Brown
K K
KK
D000085:Acetates / Q000652:urine*; D015415:Biomarkers / Q000652:urine*; D002849:Chromatography, Gas; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C487209:(2-methoxyethoxy)acetic acid; D000085:Acetates; D015415:Biomarkers