Evaluation of a new agar medium containing cetrimide, kanamycin and nalidixic acid for isolation and enhancement of pigment production of Pseudomonas aeruginosa in clinical samples.
A new selective agar medium (CKNA), containing cetrimide 0.3 g/l, kanamycin sulfate 50 mg/l, and nalidixic acid 5 mg/l, was developed for the isolation of Pseudomonas aeruginosa. It was compared to Nalidixic Acid Cetrimide agar (NAC), Pseudomonas Aeruginosa Selective Agar (PASA) and Pseudosel(TM) agar (CET) using 1,148 clinical specimens. The sensitivities rates of P. aeruginosa with CKNA, NAC, PASA, and CET were 88.2%, 81.3%, 79.2%, and 84.0%, respectively. The specificities of CKNA, NAC, PASA, and CET were 99.2%, 98.4%, 99.2%, and 99.7%, respectively.
Kodaka
Hidemasa
H
Department of Microbiology, School of Medicine, Toho University, 5-21-16, Omori-nishi, Otaku, Tokyo 143-8540, Japan. h-kodaka@yki.nissui-pharm.jp
Iwata
Morihiro
M
Yumoto
Shigeo
S
Kashitani
Fusako
F
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry*; D007612:Kanamycin / Q000494:pharmacology; D009268:Nalidixic Acid / Q000494:pharmacology; D009842:Oligopeptides; D009930:Organic Chemicals; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; D003470:Culture Media; D009842:Oligopeptides; D009930:Organic Chemicals; D010860:Pigments, Biological; D009268:Nalidixic Acid; D007612:Kanamycin; C042453:pyoverdin; D000362:Agar; C042454:pyorubin; D000077286:Cetrimonium