title: Nitric oxide regulation of L-arginine uptake in murine and human macrophages.
abstract: L-arginine uptake systems in macrophages play a role in regulating nitric oxide synthesis via the inducible L-arginine nitric oxide pathway. This paper describes the association of L-arginine transport with nitric oxide production in human peripheral blood monocyte-derived macrophages and in peritoneal macrophages from control and inducible nitric oxide synthase knock out C57BL6 mice. Experiments performed with human macrophages suggested that little or no nitric oxide was produced in human macrophages in vitro and that human macrophages exhibit a different arginine transport-specific response to stimuli compared with rodent macrophages. We conclude that increased L-arginine transport in both human and murine macrophages is dependent on the requirement for intracellular nitric oxide.
authors.affiliation: Department of Medicine, National Tuberculosis Center, and Center for Emerging and Re-emerging Pathogens, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark NJ 07103, USA.
authors.forename: Vishwanath
authors.initials: V
authors.lastname: Venketaraman
authors.forename: Meliza T
authors.initials: MT
authors.lastname: Talaue
authors.forename: Yaswant K
authors.initials: YK
authors.lastname: Dayaram
authors.forename: Marcy A
authors.initials: MA
authors.lastname: Peteroy-Kelly
authors.forename: Wei
authors.initials: W
authors.lastname: Bu
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Connell
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport; D002478:Cells, Cultured; D006801:Humans; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009163:Mycobacterium bovis / Q000254:growth & development; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000502:physiology; D052247:Nitric Oxide Synthase Type II; D013045:Species Specificity
chemical_list: D009569:Nitric Oxide; D001120:Arginine; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse