title: In vitro regeneration of roots of Phyla nodiflora and Leptadenia reticulata, and comparison of roots from cultured and natural plants for secondary metabolites.
abstract: Adventitious roots, generated using leaf explants of P. nodiflora, and meristem explants of L. reticulata, were cultured on Murashige and Skoog (MS) medium supplemented with napthylacetic acid (2 microM) and indole butyric acid (3 microM) respectively. After 30 days, subculturing of roots in liquid MS medium with napthylacetic acid (1.5 microM) for P. nodiflora and indole butyric acid (3 microM) for L. reticulata afforded considerable increase in root mass. HPTLC profiles and microscopic examination of transverse sections of in vitro and naturally grown roots provided information on secondary metabolite accumulation vis-à-vis developmental stages of the root.
authors.affiliation: Pharmacy Department, The M S University of Baroda, Faculty of Technology and Engineering, Kala-bhavan, Vadodara 390 001, India.
authors.forename: Trupti
authors.initials: T
authors.lastname: Bhatt
authors.forename: Vandana
authors.initials: V
authors.lastname: Jain
authors.forename: M G
authors.initials: MG
authors.lastname: Jayathirtha
authors.forename: G
authors.initials: G
authors.lastname: Banerjee
authors.forename: S H
authors.initials: SH
authors.lastname: Mishra
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000502:physiology*; D002855:Chromatography, Thin Layer; D018517:Plant Roots / Q000254:growth & development*; D012038:Regeneration; D029648:Verbenaceae / Q000502:physiology*