title: The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153.
abstract: Nuclear envelope breakdown is a critical step in the cell cycle of higher eukaryotes. Although integral membrane proteins associated with the nuclear membrane have been observed to disperse into the endoplasmic reticulum at mitosis, the mechanisms involved in this reorganization remain to be fully elucidated. Here, using Xenopus extracts, we report a role for the COPI coatomer complex in nuclear envelope breakdown, implicating vesiculation as an important step. We have found that a nuclear pore protein, Nup153, plays a critical role in directing COPI to the nuclear membrane at mitosis and that this event provides feedback to other aspects of nuclear disassembly. These results provide insight into how key steps in nuclear division are orchestrated.
authors.affiliation: Department of Oncological Sciences, Huntsman Cancer Institute, 2000 Circle of Hope, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Prunuske
authors.forename: Ammon M
authors.initials: AM
authors.lastname: Fager
authors.forename: Katharine S
authors.initials: KS
authors.lastname: Ullman
mesh_terms: D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D020755:Coat Protein Complex I / Q000502:physiology*; D020756:Coatomer Protein / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D034882:Lamins / Q000378:metabolism; D018613:Microscopy, Confocal; D009685:Nuclear Envelope / Q000502:physiology*; D028861:Nuclear Pore Complex Proteins / Q000502:physiology*; D010063:Ovum; D010446:Peptide Fragments; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000737:chemistry; D016622:Silver Staining; D014981:Xenopus; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D020755:Coat Protein Complex I; D020756:Coatomer Protein; D016213:Cyclins; D034882:Lamins; C079377:NUP153 protein, human; D028861:Nuclear Pore Complex Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D020727:ADP-Ribosylation Factors