Ichthyotoxic and anticarcinogenic effects of triterpenoids from Sandoricum koetjape bark.
After bioassay-guided fractionation of the extract from Sandoricum koetjape bark, which exhibited significant toxicity to killifish (Oryzias latipes), two ichthyotoxic triterpenoids were isolated and characterized as koetjapic acid and 3-oxo-olean-12-en-29-oic acid. These constituents, along with non-toxic katonic acid, had a remarkable inhibitory effect on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA), which is a preliminary in vitro screening method for possible anti-tumor-promoting agents. Of the triterpenoids active in vitro, koetjapic acid appears to be a promising cancer chemopreventive agent, since it significantly delayed tumor promotion in two-stage mouse skin carcinogenesis induced by 7,12-dimethylbenz(a)anthracene and promoted by TPA.
Ismail
Intan Safinar
IS
Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Ito
Hideyuki
H
Mukainaka
Teruo
T
Higashihara
Hiroshi
H
Enjo
Fumio
F
Tokuda
Harukuni
H
Nishino
Hoyoku
H
Yoshida
Takashi
T
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D019309:Epstein-Barr Virus Nuclear Antigens / Q000187:drug effects; D005260:Female; D023781:Fundulidae / Q000502:physiology*; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity*
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D019309:Epstein-Barr Virus Nuclear Antigens; D010936:Plant Extracts; D014315:Triterpenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate