title: A recombinant vaccine expressing a mammalian Mycobacterium sp. antigen is immunostimulatory but not protective in striped bass.
abstract: A recombinant vaccine was constructed for piscine mycobacteriosis utilizing a Brucella abortus strain RB51 vector expressing a mammalian Mycobacterium sp. 85A antigen. Juvenile striped bass were inoculated with the resulting construct at doses equivalent to 10(6), 10(7), 10(8), 10(9), and 10(10) colony-forming units/fish. Blood and tissue samples from these fish demonstrated significant specific humoral and cell-mediated immune responses towards the 85A antigen in a dose-dependent manner. However, survival studies determined that inoculated fish failed to demonstrate cross-protective responses after live Mycobacterium marinum challenge 70 days post-inoculation.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine (VMRCVM), Virginia Polytechnic Institute and State University (VPI&SU), Duck Pond Drive, Blacksburg, VA 24061, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Pasnik
authors.forename: Ramesh
authors.initials: R
authors.lastname: Vemulapalli
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Smith
authors.forename: Gerhardt G
authors.initials: GG
authors.lastname: Schurig
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D001483:Base Sequence; D001492:Bass; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D007114:Immunization / Q000662:veterinary*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000276:immunology*; D009164:Mycobacterium Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D016415:Sequence Alignment; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000276:immunology / Q000592:standards
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic