title: Sarcocystis inghami n. sp. (Sporozoa: Sarcocystidae) from the skeletal muscles of the Virginia opossum Didelphis virginiana in Michigan.
abstract: This report describes the newly identified Sarcocystis inghami n. sp. from the skeletal muscles of opossums (Mammalia: Didelphidae) that were collected from south central Michigan (42 degrees 43'-42 degrees 79'N, 84 degrees 18'-84 degrees 86'W), USA. The new species is distinguished from all species described from North and South American opossums by the distinctive morphology of the villar protrusions on the cyst wall. Sarcocysts of S. inghami are microscopic, up to 700 microm long and 110 microm wide. The sarcocyst wall is up to 7 microm thick, with long, stalked protrusions which average 5.5 x 1.2 microm. These are constricted at the base, expanded laterally, rounded off distally and occasionally bifid. The villar protrusions have numerous microtubules without electron-dense bodies that extend from the tips into the granular layer. Bradyzoites are 10.7 x 4.3 (8-12 x 4-5) microm. This is the second species of Sarcocystis sarcocyst described from the Virginia opossum in North America.
authors.affiliation: Molecular Epidemiology Laboratory, College of Veterinary Medicine, Michigan State University, MI 48824, USA.
authors.forename: Hany M
authors.initials: HM
authors.lastname: Elsheikha
authors.forename: Scott D
authors.initials: SD
authors.lastname: Fitzgerald
authors.forename: Linda S
authors.initials: LS
authors.lastname: Mansfield
authors.forename: A Mahdi
authors.initials: AM
authors.lastname: Saeed
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D006651:Histocytochemistry; D008824:Michigan; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000469:parasitology; D009893:Opossums / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012522:Sarcocystis / Q000648:ultrastructure*
chemical_list: D016054:DNA, Protozoan