Analysis of plant sterol and stanol esters in cholesterol-lowering spreads and beverages using high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy.
Plant sterol and stanol esters were separated on a Luna hexyl-phenyl column using a gradient of acetonitrile (90-100%) in water. The eluted compounds were detected by atmospheric pressure chemical ionization (APCI)-mass spectroscopy (MS) in the positive mode. Sterol and stanol esters produced [M + H - HOOCR](+) ions. Application of the hyphenated technique-LC-MS-allowed differentiation between a number of esters of sitosterol, campesterol, stigmasterol, and (tentatively) avenasterol, as well as sitostanol and campestanol esters. With cholesteryl decanoate used as the internal standard, the method showed good linearity, precision, and reproducibility. The method required minimal sample pretreatment and can be applied to samples with high water content (juices) as well as samples with high oil content (margarine spreads). The method could be useful for the analysis of sterol and stanol esters in fortified food products.
Mezine
Igor
I
A. M. Todd Group, 150 Domorah Drive, Montgomeryville, PA 18936, USA. imezine@amtbotanicals.com
Zhang
Huizhen
H
Macku
Carlos
C
Lijana
Robert
R
D000924:Anticholesteremic Agents; D001628:Beverages / Q000032:analysis*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus; D004952:Esters / Q000032:analysis; D005638:Fruit; D008383:Margarine / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010840:Phytosterols / Q000032:analysis*; D012680:Sensitivity and Specificity; D012855:Sitosterols / Q000032:analysis / Q000737:chemistry*; D013265:Stigmasterol / Q000032:analysis
D000924:Anticholesteremic Agents; D004952:Esters; D010840:Phytosterols; D012855:Sitosterols; C407042:plant stanol ester; C003535:campestanol; C021273:campesterol; C025473:gamma-sitosterol; D008383:Margarine; D002784:Cholesterol; D013265:Stigmasterol; C021255:stigmastanol