title: Lack of association between interleukin-10 gene promoter polymorphism, -1082G/A, and severe malaria in Thailand.
abstract: Interleukin-10 (IL-10) is an important cytokine in the down-regulation of inflammatory responses, and it has been reported that a low plasma concentration of IL-10 is associated with severe anemia and cerebral malaria in Plasmodium falciparum infections. The IL-10 gene is located on chromosome 1q31-32, and a promoter polymorphism (-1082G/A) is known to affect IL-10 protein production. In order to examine the possible association of the -1082G/A polymorphism with the severity of malaria, we studied 203 mild malaria, 164 non-cerebral severe malaria, and 109 cerebral malaria patients living in northwest Thailand. The genotyping was performed by a fluorescence resonance energy transfer (FRET) method. The frequencies of a major allele -1082A in mild malaria, in non-cerebral severe malaria, and in cerebral malaria patients were 92.6%, 92.1%, and 92.7% respectively. Our results showed no significant association of the -1082G/A polymorphism with the severity of malaria.
authors.affiliation: Department of Human Genetics, Graduate School of Medicine, University of Tokyo, Japan. juno@m.u-tokyo.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Ohashi
authors.forename: Izumi
authors.initials: I
authors.lastname: Naka
authors.forename: Jintana
authors.initials: J
authors.lastname: Patarapotikul
authors.forename: Hathairad
authors.initials: H
authors.lastname: Hananantachai
authors.forename: Sornchai
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: Katsushi
authors.initials: K
authors.lastname: Tokunaga
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016778:Malaria, Falciparum / Q000235:genetics* / Q000503:physiopathology; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012720:Severity of Illness Index; D013785:Thailand
chemical_list: D017931:DNA Primers; D016753:Interleukin-10