Molecular cloning and characterization of a 79-kDa iron-repressible outer-membrane protein of Moraxella bovis.
Moraxella bovis expresses an iron-repressible 79-kDa outer-membrane protein, IrpA. DNA and N-terminal amino acid sequence analysis indicate that IrpA is closely related to FrpB of Neisseria meningitidis, FetA of Neisseria gonorrhoeae and CopB of Moraxella catarrhalis. The results of manganese mutagenesis and a gel-shift assay suggested that the transcription of irpA is negatively regulated by the ferric uptake regulator. The insertion of an antibiotic resistance cassette into the irpA gene affected the strain's ability to utilize bovine transferrin and lactoferrin. IrpA was detected in geographically diverse clinical isolates, and the antigenicity of IrpA was conserved in all the isolates tested. Therefore, IrpA may have potential as a candidate vaccine.
Kakuda
Tsutomu
T
Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628, Japan. kakuda@vmas.kitasato-u.ac.jp
Oishi
Daiki
D
Tsubaki
Shiro
S
Takai
Shinji
S
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016955:Moraxella bovis / Q000235:genetics* / Q000378:metabolism*; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D014168:Transferrin / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C093120:FrpB protein, bacteria; D012097:Repressor Proteins; D014168:Transferrin; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron; D007781:Lactoferrin