Misidentification of Aeromonas veronii biovar sobria as Vibrio alginolyticus by the Vitek system.
OBJECTIVE
To find the cause of misidentification of aeromonads when using the Vitek system.


RESULTS
Two Aeromonas veronii biovar sobria isolates were misidentified as Vibrio alginolyticus by the Vitek system. Both strains' identification was confirmed by biochemical testing, API 20E/20NE kits and/or 16S RFLP analysis. Thirty-one known Aeromonas species were tested by the Vitek system using 0.45 and 0.85% saline in the suspension medium. It was not clear whether low salinity causes misidentification of Aeromonas species more frequently.


CONCLUSIONS
The specified reaction time may be inappropriately short for some critical biochemical tests of some strains. An ingenious reading strategy regarding incubation time is necessary to improve identification of Aeromonas species by the Vitek system.


CONCLUSIONS
To our knowledge, this is the first report of misidentification of A. veronii biovar sobria as V. alginolyticus in the Vitek system.
Park
T S
TS
Department of Laboratory Medicine and Internal Medicine, College of Medicine, Pusan National University, Busan, Korea.
Oh
S H
SH
Lee
E Y
EY
Lee
T K
TK
Park
K H
KH
Figueras
M J
MJ
Chang
C L
CL
D000333:Aeromonas / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology; D015373:Bacterial Typing Techniques; D003951:Diagnostic Errors; D017809:Fatal Outcome; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012965:Sodium Chloride; D014735:Vibrio Infections / Q000175:diagnosis / Q000382:microbiology; D044149:Vibrio alginolyticus / Q000145:classification / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic; D012965:Sodium Chloride