Absorptive activity of calcium in the isolated cecal epithelium adaptively increased by 2 week's feeding of difructose anhydride III in rats.
We compared net Ca absorption and Lucifer Yellow (LY), a paracellular passage dye, permeability in the epithelium isolated from the rat small intestine, cecum, and colon after feeding with control and difructose anhydride (DFA) III diets for 14 days using the Ussing chamber system. Feeding of DFA III increased net Ca transport and LY passage in the cecal but not in small intestinal or colonic epithelium. Ability of paracellular Ca passage via Tight-junction (TJ) in the cecum was changed adaptively by feeding of DFA III. Changes in microbial fermentation may affect the functional changes of Ca transport in cecal epithelium itself.
Mineo
Hitoshi
H
Northern Advancement Center for Science and Technology, Colabo-Hokkaido, Sapporo 001-0021, Japan.
Amano
Midori
M
Chiji
Hideyuki
H
Shigematsu
Norihiro
N
Tomita
Fusao
F
Hara
Hiroshi
H
D000821:Animal Feed; D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D002432:Cecum / Q000187:drug effects / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D004187:Disaccharides; D007546:Isoquinolines; C087758:difructose anhydride III; C017475:lucifer yellow; D002118:Calcium