title: [Observation on tissue factor pathway during the onset of acute cerebral infarction].
abstract: OBJECTIVE
  To establish the possible relationship between some coagulation factors and the onset of acute cerebral infarction (ACI).
  
  
  METHODS
  The study population consisted of 71 patients with ACI confirmed by CT and 50 age-matched healthy volunteers. Blood samples were obtained during the onset period of ACI. Tissue factor (TF) and tissue factor pathway inhibitor (TFPI) activity in plasma were assayed with the chromogenic assay. Plasma TF and TFPI antigen were measured with enzyme linked immunoadsorbent assay (ELISA). Plasma F VII coagulation activity (F VII: C) and F VIII coagulation activity (F VIII: C) were developed in the one-stage system. Plasma prothrombin (FII) was determined with Ecarin assay. Plasma fibrinogen (Fbg) was measured with thrombin assay. Plasma antithrombin III activity (ATIII) was determined using heparin cofactor activity assay.
  
  
  RESULTS
  Compared with the control, plasma TF activity and antigen in patients with ACI were significantly higher (both P<0.05). But plasma TFPI activity and antigen were remarkably lower in the ACI group (both P<0.05). Plasma F VII: C was significantly higher (P<0.01), and F VIII: C was markedly lower (P<0.05). Plasma FII was remarkably higher (P<0.01). Similarly the Fbg was significantly higher in the ACI than that in the control group (P<0.01), whereas ATIII was significantly lower (P<0.01).
  
  
  CONCLUSIONS
  The initiation of TF pathway is contributed to the onset of ACI and the blood is in hypercoagulable state during the early period of ACI.
authors.affiliation: Thrombosis and Hemostasis Lab, Department of Physiology, Xiangya Medical University, Central-South University, Changsha 410078, Hunan, China. wenzhibin2002@yahoo.com.cn
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Wen
authors.forename: Shi-long
authors.initials: SL
authors.lastname: Xiong
authors.forename: Xiao-fan
authors.initials: XF
authors.lastname: He
authors.forename: Mei-xia
authors.initials: MX
authors.lastname: He
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: He
authors.forename: Qin-zhi
authors.initials: QZ
authors.lastname: Xie
authors.forename: Zai-fu
authors.initials: ZF
authors.lastname: Jian
authors.forename: Fang-ping
authors.initials: FP
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Qi-dong
authors.initials: QD
authors.lastname: Yang
mesh_terms: D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D001779:Blood Coagulation Factors / Q000032:analysis; D002544:Cerebral Infarction / Q000097:blood*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D013925:Thromboplastin / Q000032:analysis / Q000502:physiology*
chemical_list: D001779:Blood Coagulation Factors; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D013925:Thromboplastin