title: Adsorption from black tea and red wine onto in vitro salivary pellicles studied by ellipsometry.
abstract: The adsorption of black tea and red wine components onto a pellicle-like protein layer formed in vitro by adsorption from whole unstimulated saliva on hydroxyapatite discs were studied by in situ ellipsometry. It was found that components from black tea readily adsorbed to the pellicle. Subsequent exposure to saliva led to further adsorption of salivary components to give an overall increase in the amounts adsorbed. The amounts adsorbed increased still further following a third tea and saliva exposure. Components of red wine gave significantly greater amounts of adsorption to the pellicle than black tea. The adsorption of components of black tea gave a concomitant increase in colour or stain as measured by a reflectance chromameter. In all cases, the black tea- and red wine-modified pellicles were not eluted by either phosphate buffer or sodium dodecyl sulphate (SDS) rinses. Thus, black tea and red wine components have been shown to have a profound effect on in vitro pellicle maturation, causing thickened layers of stained material to build up, which are not readily removed.
authors.affiliation: Unilever Oral Care, Port Sunlight Laboratory, Bebington, Wirral, UK. andrew.joiner@unilever.com
authors.forename: Andrew
authors.initials: A
authors.lastname: Joiner
authors.forename: Dries
authors.initials: D
authors.lastname: Muller
authors.forename: Ulla M
authors.initials: UM
authors.lastname: Elofsson
authors.forename: Martin
authors.initials: M
authors.lastname: Malmsten
authors.forename: Thomas
authors.initials: T
authors.lastname: Arnebrant
mesh_terms: D000327:Adsorption; D003116:Color; D044622:Dental Pellicle / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*; D014075:Tooth Discoloration / Q000378:metabolism; D014920:Wine
chemical_list: D012471:Salivary Proteins and Peptides; D013662:Tea; D017886:Durapatite