title: Immunohistochemical localization of Bis protein in the rat central nervous system.
abstract: We studied the distribution of Bis (Bcl-2 interacting death suppressor) protein in the adult rat brain and spinal cord using immunohistochemistry. Immunoreactivity was observed in specific neuronal populations in distinct nuclei. The most intensely labeled cells were associated with the motor system, including most cranial nerve motor nuclei, Purkinje cells of the cerebellum, the red nucleus, and the ventral motor neurons of the spinal cord. Bis protein was also expressed in several structures associated with the ventricular system, including the subventricular zone of the lateral ventricle and its rostral extension, in the subcommissural organ, and in tanycytes, radial glial cells in the hypothalamus. Using double-labeling techniques, Bis-immunoreactive cells in the rostral migratory stream, coexpressing Bcl-2, were confirmed as glial fibrillary acidic protein-positive astrocytes comprising the glial tubes. The widespread distribution of Bis suggests that this protein has broader functions in the adult rat central nervous system than previously thought, and that it could be associated with a particular role in the rostral migratory system.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, 137-701 Seoul, Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
authors.forename: Jin-Woong
authors.initials: JW
authors.lastname: Chung
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Jeon
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology* / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D004027:Diencephalon / Q000166:cytology / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2