Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation.
The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.
Morimoto
Yuki
Y
Science of Functional Recovery and Reconstruction, Department of Molecular Genetics, Graduate School of Medicine and Dentistry, Okayama University, Shikata-cho 2-5-1, Okayama 700-8558, Japan.
Ouchida
Mamoru
M
Ozaki
Toshifumi
T
Kawai
Akira
A
Ito
Tatsuo
T
Yoshida
Aki
A
Inoue
Hajime
H
Shimizu
Kenji
K
D016475:3T3 Cells; D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D002455:Cell Division / Q000235:genetics; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D016254:Mutagenesis, Insertional; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D013584:Sarcoma, Synovial / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection
D009363:Neoplasm Proteins; D020033:Protein Isoforms; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C093961:SS18 protein, human; C501211:Ss18 protein, mouse; C093962:synovial sarcoma X breakpoint proteins; D008156:Luciferases