Monocyte chemoattractant protein-1 increases microglial infiltration and aggressiveness of gliomas.
Macrophages are thought to represent a first line of defense in anti-tumor immunity. Despite infiltration by microglial cells, however, malignant gliomas are still highly aggressive tumors. We here identify monocyte chemoattractant protein-1 (MCP-1) as a critical chemoattractant for glioma-infiltrating microglial cells. MCP-1-transfected rat CNS-1 gliomas were massively infiltrated by microglial cells. Whereas MCP-1 did not promote the growth of CNS-1 cells in vitro, intracerebral CNS-1-transfected tumors grew more aggressively than control-transfected tumors. This provides the first functional evidence that MCP-1 recruits microglial cells to gliomas and promotes their growth in vivo. Microglial cells may support rather than suppress glioma growth.
Platten
Michael
M
Laboratory of Molecular Neuro-Oncology, Department of Neurology, University of Tübingen Medical School, Hoppe-Seyler-Strasse 3, 72076 Tübingen, Germany.
Kretz
Alexandra
A
Naumann
Ulrike
U
Aulwurm
Steffen
S
Egashira
Kensuke
K
Isenmann
Stefan
S
Weller
Michael
M
D000818:Animals; D018932:Chemokine CCL2 / Q000502:physiology*; D002633:Chemotaxis; D003342:Corpus Striatum / Q000637:transplantation; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D005910:Glioma / Q000378:metabolism; D017628:Microglia / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection; D014407:Tumor Cells, Cultured
D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D012333:RNA, Messenger