title: Evaluation of counterions for electrospray ionization mass spectral analysis of a highly sulfated carbohydrate, sucrose octasulfate.
abstract: A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other suffated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1 degree, 2 degrees, and 3 degrees ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.
authors.affiliation: Division of Medicinal and Natural Products Chemistry, Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA.
authors.forename: Nur Sibel
authors.initials: NS
authors.lastname: Gunay
authors.forename: Keiko
authors.initials: K
authors.lastname: Tadano-Aritomi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toida
authors.forename: Ineo
authors.initials: I
authors.lastname: Ishizuka
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D002412:Cations; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D002412:Cations; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; C053081:sucrose octasulfate