title: In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells.
abstract: The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for reliable growth of E. ruminantium. Growth of the Welgevonden stock in BHK and CHO-K1 cells could lead to the development of suspension cultures suitable for the mass production of E. ruminantium for an inactivated elementary body vaccine.
authors.affiliation: Parasitology Division, Onderstepoort Veterinary Institute Private Bag X05, Onderstepoort, 0110 South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Zweygarth
authors.forename: A I
authors.initials: AI
authors.lastname: Josemans
mesh_terms: D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002460:Cell Line / Q000382:microbiology*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D016995:Ehrlichia ruminantium / Q000254:growth & development* / Q000472:pathogenicity; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology; D006357:Heartwater Disease / Q000382:microbiology