Anti-inflammatory properties of Bridelia ferruginea stem bark. Inhibition of lipopolysaccaride-induced septic shock and vascular permeability.
The anti-inflammatory activity of the aqueous extract of Bridelia ferruginea stem bark was further evaluated in models which are mediated by tumour necrosis factor-alpha (TNFalpha). The effect of the extract on lipopolysaccharide (LPS)-induced septic shock was evaluated by measuring the number of deaths and the levels of serum alanine and aspartate aminotransferases following intraperitoneal injection of LPS (1 microg/kg) into D-galactosamine-primed mice. LPS-induced vascular permeability on the back skin of mice was measured by the local accumulation of Evan's blue after subcutaneous injection of LPS. Pre-treatment with Bridelia ferruginea extract (10-80 mg/kg) produced a dose-dependent inhibition of the septic shock syndrome in mice, with 80 mg/kg of the extract exhibiting comparable activity as pentoxifylline (100 mg/kg). LPS-induced dye leakage in the skin of mice was also suppressed by the extract (10-80 mg/kg). Our study suggests that one of the mechanisms of the anti-inflammatory effects of Bridelia ferruginea possibly involve the suppression of TNFalpha up-regulation.
Olajide
Olumayokun A
OA
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. maylajide@hotmail.com
Okpako
David T
DT
Makinde
Janet M
JM
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002199:Capillary Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae / Q000737:chemistry*; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012772:Shock, Septic / Q000188:drug therapy* / Q000503:physiopathology; D012867:Skin / Q000098:blood supply / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D010936:Plant Extracts