title: [Metabolism of roxithromycin in dogs].
abstract: OBJECTIVE
  To investigate the metabolic profile of roxithromycin in dogs and the effects of oral and intravenous administrations on the metabolism of roxithromycin.
  
  
  METHODS
  Liquid chromatography-tandem mass spectrometry (LC-MSn) was used for separation and analysis of roxithromycin and its metabolites in dog bile after an oral dose or intravenous dose of roxithromycin. The metabolites were identified by comparisons of their mass spectra and LC behaviors with the references.
  
  
  RESULTS
  Totally 13 metabolites were detected in dog bile, including N-demethylated derivatives, N, N-didemethylated derivatives, O-dealkylether derivatives, decladinose derivatives, and the geometric isomers of parent drug and its metabolites.
  
  
  CONCLUSIONS
  Roxithromycin underwent 4 metabolic pathways in which geometric isomerization and decladinose metabolism were found to be markedly different between the two administration routes.
authors.affiliation: Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Shu-qiu
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.forename: Da-fang
authors.initials: DF
authors.lastname: Zhong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000378:metabolism*; D001646:Bile / Q000378:metabolism*; D001711:Biotransformation; D002853:Chromatography, Liquid; D004285:Dogs; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D015575:Roxithromycin / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015575:Roxithromycin