[Allogenic transplantation studies on human fetal pancreatic nestin positive cells].
Human fetal pancreatic nestin positive cells cultured in vitro could self assemble to form islet-like cell clusters(ICC). They are multipotential and are capable of generating insulin-producing cells. To investigate their biological character and physiological function in vivo, ICCs were implanted into NOD-Scid mice subcapsularly. The results showed that: (1) Neovascularizations were observed in implant sites. (2) Blood glucose levels of diabetic mice were reduced markedly after implantation of ICCs. (3) ICCs in non-diabetic mice proliferated abnormally and infiltrated into renal parenchyma with many cell structures formed.
Huang
Hai Xia
HX
Department of Cell Biology, Shanghai Second Medical University, Shanghai 200025.
Huang
Xin Zhi
XZ
Chen
Xin Yu
XY
Chen
Yu Ying
YY
Tang
Xue Ming
XM
D000818:Animals; D002454:Cell Differentiation; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005333:Fetus / Q000166:cytology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D016513:Mice, SCID; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D033581:Stem Cell Transplantation; D014183:Transplantation, Heterologous
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin