title: Effects of polyamines on histone polymerization.
abstract: It is generally accepted that the nucleosome structure is not static, and that alternative conformations are adopted in response to several stimuli associated with the different functions. Histones are substrates for transglutaminase (TGase), and polymerized histone and polyamine binding histone have been suggested to play important roles in nucleus. We examined whether histone polymerization catalyzed by TGase was influenced by polyamines such as putrescine (PUT), spermidine (SPD), and spermine (SPM). PUT inhibited histone polymerization, and SPD slightly prevented it. However, SPM slightly enhanced histone polymerization. These results indicate that the nuclear accumulation of the polyamines may play an important role in nuclear remodeling by histone modification. We speculate that histone cross-linking by TGase may be involved in the chromatin structure. Also, we propose that histone cross-linking by TGase may be responsible for the changes in DNA function such as transcription and replication and that TGase may be involved in cell growth and differentiation.
authors.affiliation: Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan. n-sato@tohoku-pharm.ac.jp
authors.forename: Naoko
authors.initials: N
authors.lastname: Sato
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ohtake
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kato
authors.forename: Shinya
authors.initials: S
authors.lastname: Abe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kohno
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Ohkubo
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002384:Catalysis / Q000187:drug effects; D006168:Guinea Pigs; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D001704:Biopolymers; D006657:Histones; D011073:Polyamines; D013096:Spermine; D011503:Transglutaminases; D002118:Calcium; D013095:Spermidine; D011700:Putrescine