Total parenteral nutrition entrains the central and peripheral circadian clocks.
The administration schedule of total parenteral nutrition (TPN) ignores the human physiological food-intake rhythm. This study examined the effects of TPN on the central and peripheral circadian clocks. Male Wistar rats were divided into a control group, a nocturnal-TPN group, and a diurnal-TPN group. On the seventh day after TPN administration, expression of rat period2 (rPer2) and D-site binding protein (rDBP) mRNA were measured in the suprachiasmatic nucleus (SCN) and liver. While rPer2 and rDBP mRNA expression of the nocturnal-TPN rats showed similar oscillation patterns to those in the free-fed control rats both in the SCN and liver, they were shifted significantly in the diurnal-TPN rats. This phase shift occurred as early as day 1. TPN, which ignores physiological food-intake rhythms, alters the rhythm of the central and peripheral clocks.
Miki
Hirofumi
H
Department of Surgery and Clinical Oncology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Yano
Masahiko
M
Iwanaga
Hiroshi
H
Tsujinaka
Toshimasa
T
Nakayama
Mitsuo
M
Kobayashi
Masaru
M
Oishi
Katsutaka
K
Shiozaki
Hitoshi
H
Ishida
Norio
N
Nagai
Katsuya
K
Monden
Morito
M
D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D002490:Central Nervous System / Q000502:physiology*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D004076:Digoxigenin / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D010289:Parenteral Nutrition, Total; D017933:Peripheral Nervous System / Q000502:physiology*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000502:physiology
D009687:Nuclear Proteins; D012333:RNA, Messenger; D012313:RNA; D004076:Digoxigenin