NTP concentration switches transcriptional activity by changing the large-scale structure of DNA.
It is becoming clearer that genetic activity is closely associated with the intracellular energy state. However, the mechanisms of this association are still unclear. In this study, we focused on large-scale changes in the structure of DNA to examine the effect of the NTP concentration on the transcription reaction with T7 RNA polymerase and compared the results with long duplex DNA to those with a short persistent-length(1) fragment. The transcriptional activity dramatically changed only for long duplex DNA within a narrow range of NTP concentrations associated with changes in the large-scale structure of DNA. This result suggests that the energy state may play an essential role in regulating ON/OFF switching on transcriptional activity.
Akitaya
Tatsuo
T
Department of Physics, Graduate School of Science, Kyoto University, and CREST, Kyoto 606-8502, Japan.
Tsumoto
Kanta
K
Yamada
Ayako
A
Makita
Naoko
N
Kubo
Koji
K
Yoshikawa
Kenichi
K
D001435:Bacteriophages; D004247:DNA / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009711:Nucleotides / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins
D009711:Nucleotides; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases