Enrichment for isolating Salmonella Choleraesuis and other Salmonella spp. from pigs.
The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42 degrees C. As the enrichment in RV at 37 degrees C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37 degrees C and HTT enrichment at 42 degrees C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42 degrees C (43.4%) than with RV at 37 degrees C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.
Osumi
Takayuki
T
Zen-noh Institute of Animal Health, Sakura, Chiba, Japan.
Asai
Tetsuo
T
Namimatsu
Takanori
T
Sato
Shizuo
S
Yamamoto
Koshi
K
D000818:Animals; D003470:Culture Media; D005843:Geography; D012475:Salmonella / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D013045:Species Specificity; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology*
D003470:Culture Media