title: Long-lasting changes in the T-cell receptor V beta repertoires of CD4 memory T-cell populations in the peripheral blood of radiation-exposed people.
abstract: To study the long-term effects of radiation-induced T-cell depletion on the T-cell receptor (TCR) Vbeta repertoires of human peripheral CD4 T-cell populations, we measured the percentages of CD4 T cells representing each of the full range of possible TCR Vbeta families in a cohort of atomic bomb survivors. We then estimated the extent to which the expression levels for individual TCR Vbeta families differed from the average expression level for that particular TCR Vbeta family across the entire cohort. We found no evidence of a systematic change in the TCR Vbeta repertoires of the naïve CD4 T-cell populations, but memory CD4 T-cell TCR Vbeta family expression levels diverged significantly from the population average for counterpart families, especially in individuals who had been exposed to higher doses and were at least 20 years of age at the time of the bombing. Comparisons of the TCR Vbeta family expression profiles in the naïve and memory CD4 T-cell pools of the same group of adult survivors revealed that differences in the TCR Vbeta repertoires of these two types of CD4 T-cell pool were larger in more heavily exposed survivors than in unexposed controls. These findings suggest that the memory CD4 T-cell pools of individuals who received significant radiation doses in adulthood may well have become (and could still be) dependent upon a much less diverse complement of TCR Vbeta families than would otherwise have been the case.
authors.affiliation: Departments of Radiobiology/Molecular Epidemiology and Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan. ykusunok@rerf.or.jp
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kusunoki
authors.forename: Mika
authors.initials: M
authors.lastname: Yamaoka
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Kasagi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Hayashi
authors.forename: Donald G
authors.initials: DG
authors.lastname: MacPhee
authors.forename: Seishi
authors.initials: S
authors.lastname: Kyoizumi
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000528:radiation effects; D007564:Japan; D008297:Male; D008875:Middle Aged; D009689:Nuclear Warfare; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood / Q000528:radiation effects*; D017741:Survivors; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000528:radiation effects*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta