title: HBO suppresses Nogo-A, Ng-R, or RhoA expression in the cerebral cortex after global ischemia.
abstract: Nogo-A, a myelin-associated neurite outgrowth inhibitory protein, binds with the Ng-R receptor to activate RhoA intracellular signals and inhibit the plasticity after CNS injury. We evaluated the effect of hyperbaric oxygen (HBO) on the expression of Nogo-A, Ng-R, and RhoA after transient global ischemia in a rat 2 vessel occlusion global ischemic model. Male SD rats (n=78) were randomly divided into 13 groups: 1 sham group, 6 groups of global ischemia, and 6 groups of HBO treatment after global ischemia. HBO (3ATA) was applied for 2 hr at 1 hr after global ischemia. Rats were sacrificed at 6, 12, 24, 48, and 96 hr and 7 days. Global ischemia (10 min) produced a marked increase of Nogo-A/B, Nogo-A, Ng-R, and RhoA expression. Immunohistochemistry showed increased Nogo-A/B and Nogo-A located in the myelin sheath of ischemic brain cortex. Ng-R expressed on the surface of neurons and their processes, and RhoA expressed inside the cytoplasm of neurons in ischemic brain. HBO significantly reduced neurological injury, decreased the levels of Nogo-A, Ng-R, and RhoA in ischemic injured cortex (p<0.05).
authors.affiliation: Department of Neurosurgery, Louisiana State University Health Sciences Center-Shreveport, Shreveport, LA, USA.
authors.forename: Changman
authors.initials: C
authors.lastname: Zhou
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Anil
authors.initials: A
authors.lastname: Nanda
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D002452:Cell Count; D002470:Cell Survival; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D058851:GPI-Linked Proteins; D006931:Hyperbaric Oxygenation; D008297:Male; D009185:Myelin Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide / Q000378:metabolism*; D012016:Reference Values; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070798:Nogo Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C000603197:Rtn4 protein, rat; C486581:Rtn4r protein, rat; D020742:rhoA GTP-Binding Protein