Intraportal donor bone marrow transplantation improves intestinal allograft survival in rats under FK506-based immunosuppression.
Donor-specific immunosuppression is important in transplant surgery. We examined the effect of intraportal donor-specific bone marrow transplantation on heterotopic small bowel transplantation in the high responder rat combination, ACI to Lewis. The study comprised five treatment groups: untreated controls (group 1); FK506 alone (group 2); low-dose predonine + FK506 (group 3); high-dose predonine + FK506 (group 4); and intraportal donor-specific bone marrow transplantation + FK506 (group 5). Intraportal transplantation was performed pre-operatively and FK506 and predonine given post-operatively. Intestinal allograft survival and changes of intragraft cytokine expression were analysed using the reverse transcription polymerase chain reaction. Allograft survival (mean +/- SD) was lowest in group 1 and greatest in group 5. The group 5 treatment regimen also down-regulated interferon-gamma and interleukin-2 transcription in the transplanted intestine. Intraportal donor bone marrow transplant combined with FK506 immunosuppression was found therefore to be the most beneficial treatment regimen.
Sun
D S
DS
Department of Gastroenterological Surgery and Transplant, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Yagi
T
T
Oyama
T
T
Matsukawa
H
H
Matsuda
H
H
Sadamori
H
H
Inagaki
M
M
Matsuoka
J
J
Iwagaki
H
H
Tanaka
N
N
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D006085:Graft Survival; D006086:Graft vs Host Disease; D007108:Immune Tolerance / Q000187:drug effects*; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007421:Intestine, Small / Q000637:transplantation*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D011239:Prednisolone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D016559:Tacrolimus / Q000494:pharmacology*; D014184:Transplantation, Homologous
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007166:Immunosuppressive Agents; D011239:Prednisolone; D016559:Tacrolimus