Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples.
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.
Agüero
M
M
Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain. aguero@inia.es
Fernández
J
J
Romero
L
L
Sánchez Mascaraque
C
C
Arias
M
M
Sánchez-Vizcaíno
J M
JM
D000358:African Swine Fever Virus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D004279:DNA, Viral