title: Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1.
abstract: Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae) has been shown to possess anti-microbial, anti-tumoral, and anti-inflammatory properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. These results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage.
authors.affiliation: Medicinal Resources Research Center, School of Medicine, Wonkwang University, Iksan,, Chonbuk 570-749 South Korea.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Moon
authors.forename: Byung-Min
authors.initials: BM
authors.lastname: Choi
authors.forename: Gi-Su
authors.initials: GS
authors.lastname: Oh
authors.forename: Hyun-Ock
authors.initials: HO
authors.lastname: Pae
authors.forename: Jong-Duck
authors.initials: JD
authors.lastname: Kim
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.forename: Cheon-Sik
authors.initials: CS
authors.lastname: Oh
authors.forename: Dae-Han
authors.initials: DH
authors.lastname: Kim
authors.forename: Young-Deuk
authors.initials: YD
authors.lastname: Rho
authors.forename: Min-Kyo
authors.initials: MK
authors.lastname: Shin
authors.forename: Ho-Sub
authors.initials: HS
authors.lastname: Lee
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D015153:Blotting, Western; D002248:Carbon Monoxide / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004232:Diuretics / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D004232:Diuretics; D005960:Glucosides; D008565:Membrane Proteins; D018696:Neuroprotective Agents; D016877:Oxidants; C006928:catalposide; D002248:Carbon Monoxide; D006861:Hydrogen Peroxide; D007501:Iron; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D001663:Bilirubin