Molecular basis of constitutive production of basement membrane components. Gene expression profiles of Engelbreth-Holm-Swarm tumor and F9 embryonal carcinoma cells.
Engelbreth-Holm-Swarm (EHS) tumors produce large amounts of basement membrane (BM) components that are widely used as cell culture substrates mimicking BM functions. To delineate the tissue/organ origin of the tumor and the mechanisms operating in the BM overproduction, a genome-wide expression profile of EHS tumor was analyzed using RIKEN cDNA microarrays containing approximately 40,000 mouse cDNA clones. Expression profiles of F9 embryonal carcinoma cells that produce laminin-1 and other BM components upon differentiation into parietal endoderm-like cells (designated F9-PE) were also analyzed. Hierarchical clustering analysis showed that the gene expression profiles of EHS and F9-PE were the most similar among 49 mouse tissues/organs in the RIKEN Expression Array Database, suggesting that EHS tumor is parietal endoderm-derived. Quantitative PCR analysis confirmed that not only BM components but also the machineries required for efficient production of BM components, such as enzymes involved in post-translational modification and molecular chaperones, were highly expressed in both EHS and F9-PE. Pairs of similar transcription factor isoforms, such as Gata4/Gata6, Sox7/Sox17, and Cited1/Cited2, were also highly expressed in both EHS tumor and F9-PE. Time course analysis of F9 differentiation showed that up-regulation of the transcription factors was associated with those of BM components, suggesting their involvement in parietal endoderm specification and overproduction of the BM components.
Futaki
Sugiko
S
Sekiguchi Biomatrix Signaling Project, ERATO, Japanese Science and Technology Agency (JST), Aichi Medical University, 21 Karimata, Yazako Nagakute-cho, Aichi-gun, Aichi, 480-1195, Japan.
Hayashi
Yoshitaka
Y
Yamashita
Megumi
M
Yagi
Ken
K
Bono
Hidemasa
H
Hayashizaki
Yoshihide
Y
Okazaki
Yasushi
Y
Sekiguchi
Kiyotoshi
K
D000818:Animals; D001485:Basement Membrane / Q000737:chemistry* / Q000502:physiology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004707:Endoderm / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms; D013997:Time Factors
D018076:DNA, Complementary; D020033:Protein Isoforms