Characterization of surface oxide films on titanium and adhesion of osteoblast.
The relationship between surface characteristics of titanium and initial interactions of titanium-osteoblasts was investigated. Titanium plates were heat-treated in different oxidation atmospheres. The third passage rabbit osteoblasts were cultured on the titanium plates for 24h. After the heat-treatment, the crystal structure of the surface oxide films on titanium was identified using X-ray diffractometer and X-ray photoelectron spectroscopy (XPS). The surface roughness of titanium was measured with a profilometer. The surface energy was obtained by measurement of contact angles and calculation with Owens-Wendt-Kaeble's equation. The amount of surface hydroxyl (OH)(s) groups was examined using XPS. The change of binding energy of the some elements on the substrate surface suggested that the interactions between the cells and the titanium involved chemical reactions. The greater surface roughness, higher surface energy and more surface hydroxyl groups resulted in greater numbers of adhered osteoblasts and higher cell activity. Compared to the acidic hydroxyl (OH)(a) groups in (OH)(s) groups and the dispersion component of the total surface energy, the basic hydroxyl (OH)(b) groups and the polar component play more important roles in the osteoblast-titanium interaction.
Feng
B
B
School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. fengbh@163.com
Weng
J
J
Yang
B C
BC
Qu
S X
SX
Zhang
X D
XD
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010087:Oxides / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
D020099:Coated Materials, Biocompatible; D010087:Oxides; D014025:Titanium; D000469:Alkaline Phosphatase