title: Flow cytometric analysis of peripheral blood mononuclear cells induced by experimental endotoxemia in horse.
abstract: Cellular activation and functional cell surface markers were evaluated during experimentally-induced endotoxemia in healthy horses. Eight healthy adult horses were infused a low dose of endotoxin (lipopolysaccharide from Escherichia coli O26: B6, 30 ng/kg of body weight, IV) and five control horses were given an equivalent volume of sterile saline solution. Venous blood samples were collected for flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and to measure plasma endotoxin concentrations. Clinical signs of endotoxemia were recorded at 10, 20, 30, 40, 50 min, 1, 2, 3, 4, 8, 16, 24 and 48 hr after endotoxin or saline solution administration. Clinical findings characteristic of endotoxemia (tachycardia, tachypnea, increased rectal temperature, and leukopenia) occurred transiently in all horses administered endotoxin; however, plasma endotoxin concentrations were detectable in only 50% (4/8) of the endotoxin-infused horses. The percentage of CD4(+), CD5(+), and CD8(+) cells decreased while the percentage of CD14(+), IgM(+), and MHC class II(+) cells increased significantly after endotoxin infusion. Alterations in the immunophenotype of PBMCs from horses with experimentally-induced endotoxemia were associated with changes in vital signs, indicating that endotoxin altered the immuno balance.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.forename: Kan-ichi
authors.initials: K
authors.lastname: Kusano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Miyake
authors.forename: Shigeo
authors.initials: S
authors.lastname: Fukuda
authors.forename: Junkichi
authors.initials: J
authors.lastname: Takahashi
authors.forename: Miho
authors.initials: M
authors.lastname: Inotsume
authors.forename: Shiro
authors.initials: S
authors.lastname: Hirano
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Yoshihara
authors.forename: Ramiro E
authors.initials: RE
authors.lastname: Toribio
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Tomo-o
authors.initials: TO
authors.lastname: Yoshino
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000097:blood; D001831:Body Temperature; D019446:Endotoxemia / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000097:blood; D006734:Horse Diseases / Q000097:blood* / Q000503:physiopathology / Q000628:therapy; D006736:Horses; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D012143:Respiratory Physiological Phenomena
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II