Increased seroreactivity in tic disorder patients to a 60 kDa protein band from a neuronal cell line.
In tic disorders, increased seroreactivity against neuronal antigens has been demonstrated, without performing molecular characterization of antigens. Here, unselected patients with a tic disorder were compared with healthy controls, autistic disorder (AD), and obsessive-compulsive disorder (OCD) patients. Seroreactivity against neuroblastoma cells was analyzed by Western blot. Anti-60 kDa binding occurred significantly more frequently in tic disorder patients (67.1%) than in AD (40.0%), OCD (40.0%) and healthy controls (41.9%). Sequence analysis of the 60 kDa protein band identified this as a ubiquitous heat shock protein. However, the involvement of other autoantigens with a molecular weight of 60 kDa cannot be excluded.
Hoekstra
Pieter J
PJ
Child and Adolescent Psychiatry Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. Pieter.Hoekstra@kjpnn.nl
Horst
Gerda
G
Limburg
Pieter C
PC
Troost
Pieter W
PW
van Lang
Natasja
N
de Bildt
Annelies
A
Korf
Jakob
J
Kallenberg
Cees G M
CG
Minderaa
Ruud B
RB
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000276:immunology* / Q000473:pathology; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D012720:Severity of Illness Index; D013981:Tic Disorders / Q000097:blood / Q000276:immunology*
D001323:Autoantibodies; D018834:Chaperonin 60; D007074:Immunoglobulin G; D010446:Peptide Fragments