Identification of oral peptostreptococcus isolates by PCR-restriction fragment length polymorphism analysis of 16S rRNA genes.
Oral Peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of PCR amplification of 16S rRNA genes and restriction enzyme analysis of the amplified products. This method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.
Riggio
Marcello P
MP
Infection and Immunity Research Group, University of Glasgow Dental School, Glasgow G2 3JZ, United Kingdom. m.riggio@dental.gla.ac.uk
Lennon
Alan
A
D005232:Fatty Acids, Volatile / Q000032:analysis; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D010462:Peptostreptococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S