Identification of HLA-B27-restricted peptides in reactive arthritis and other spondyloarthropathies: computer algorithms and fluorescent activated cell sorting analysis as tools for hunting of HLA-B27-restricted chlamydial and autologous crossreactive peptides involved in reactive arthritis and ankylosing spondylitis.
The illustrated clinical and experimental results demonstrate the strong relationship between the MHC class I antigen HLA-B27 and synovial CD8+ T cells with specificity for bacterial and possible self-antigen in SpA. These new aspects obtained in recent experimental and clinical studies might also provide clues to the pathomechanisms of joint inflammation in SpA. In particular, the newly developed techniques will be of great relevance in the near future. New and more precise bioalgorithms reflecting new insights in the biology and biochemistry of proteins as recently presented [98, 99] can be helpful (e.g., a program with an improved prediction of the features of immunoproteasomes). Intracellular and secreted cytokine staining by FACScan allows examination of a great number of cells expressing certain antigens in response to certain stimuli. The analysis of T-cell responses with tetramer/peptide complexes can be useful to screen tissue sections for TCR, recognizing foreign or self-derived epitopes on those complexes loaded with selected (e.g., bacterial) peptides. Identification of arthritogenic peptides and a further understanding of the immunology of the pathomechanisms in SpA might open ways to design new peptide vaccines to prevent inflammation, autoimmunity, and other diseases by early intervention [100].
Kuon
Wolfgang
W
Section of Rheumatology, FU-Klinikum Benjamin Franklin, Berlin Hindenburgdamm 30, 12200 Berlin, Germany. kuon@medizin.fu-berlin.de
Sieper
Joachim
J
D000465:Algorithms; D016918:Arthritis, Reactive / Q000276:immunology* / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D002690:Chlamydia Infections / Q000150:complications* / Q000276:immunology; D003429:Cross Reactions; D005434:Flow Cytometry / Q000379:methods*; D015796:HLA-B27 Antigen / Q000032:analysis* / Q000276:immunology; D006801:Humans; D012984:Software; D025242:Spondylarthropathies / Q000276:immunology / Q000382:microbiology
D001426:Bacterial Proteins; D015796:HLA-B27 Antigen