title: Monoclonal antibodies against blood group A secretors and nonsecretors saliva.
abstract: To obtain monoclonal antibodies (MAbs) that distinguish secretor and nonsecretor from their saliva in forensic casework, two (K7405 and K7422) and one (K7516) MAbs reacting to blood group A antigen were produced by immunization of mice with salivary mucin obtained from blood group A secretors and nonsecretors, respectively. K7405, produced by immunization with salivary mucin obtained from A secretor, reacted with the A substances bound to the carrier protein but not with the A substance separated from the carrier protein. On the other hand, the K7422 and K7516 were reactive to the A substance separated from carrier protein. From these results, we conclude that K7405 recognizes the A substances clustered on the carrier protein and K7422 and K7516 recognize the isolated A substance. In the forensic blood typing of body fluids, A secretors and A nonsecretors can be clearly discriminated by the combined application of two MAbs (K7405 and K7516), which react differently against saliva samples.
authors.affiliation: National Research Institute of Police Science, 6-3-1, Kashiwanoba, Kashiwa, Chiba, 277-0882, Japan. ohmori@nrips.go.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohmori
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Rie
authors.initials: R
authors.lastname: Aoi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shiraishi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ito
authors.forename: Hajime
authors.initials: H
authors.lastname: Sato
mesh_terms: D000017:ABO Blood-Group System; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009077:Mucins; D009844:Oligosaccharides; D012471:Salivary Proteins and Peptides