Standardisation and evaluation of DNA-lanthanide fluorescence spectroscopy for determining rifampicin resistance in Mycobacterium tuberculosis clinical isolates.
METHODS
Tuberculosis Research Centre, Chennai.


OBJECTIVE
To rapidly identify multidrug-resistant Mycobacterium tuberculosis using a novel method.


METHODS
A new assay, based on DNA-lanthanide fluorescence, was standardised and evaluated using 93 each of coded rifampicin-resistant and rifampicin-sensitive M. tuberculosis clinical isolates for the correct identification of rifampicin resistance. The results obtained by the new assay were compared with the conventional results.


CONCLUSIONS
The new assay gave a sensitivity and specificity of 88% and 85%, respectively. It is simple, easy to perform and requires 48 hours for the drug susceptibility results to be available after obtaining the primary culture.
Mani
C
C
Tuberculosis Research Centre (Indian Council of Medical Research), Chetpet, Chennai, India.
Selvakumar
N
N
Gajendiran
N
N
Panigrahi
B
B
Venkatesan
P
P
Narayanan
P R
PR
D000995:Antitubercular Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial; D006801:Humans; D028581:Lanthanoid Series Elements; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity*; D012016:Reference Values; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy*
D000995:Antitubercular Agents; D004269:DNA, Bacterial; D028581:Lanthanoid Series Elements; D012293:Rifampin