Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field.
During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE-negative live-attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and PCR. All the techniques used identified the isolated virus as a gE-negative BHV-1 phenotypically and genotypically identical to the Za strain used as a control.
Dispas
M
M
Department Biocontrol/Section Epidemiology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium.
Schynts
F
F
Lemaire
M
M
Letellier
C
C
Vanopdenbosch
E
E
Thiry
E
E
Kerkhofs
P
P
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000821:virology*; D017353:Gene Deletion; D005838:Genotype; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D010641:Phenotype; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins; D014765:Viral Vaccines / Q000276:immunology; D017201:Virus Shedding
D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins