title: Cytogenetic biomonitoring in petrol station attendants: micronucleus test in exfoliated buccal cells.
abstract: To study the effects of occupational exposure to petroleum derivates such as benzene, exfoliated buccal cells from 50 petrol station attendants and 50 age- and sex-matched control subjects were examined for micronucleus (MN) frequency. Frequencies of nuclear abnormalities (NA) other than micronuclei, such as binucleates, karyorrhexis and karyolysis, were also evaluated. Benzene exposure was ascertained by measuring urinary phenol levels. The mean urinary phenol level of station workers was found to be significantly higher than that of control subjects (P < 0.05). Analysis of buccal cells revealed that MN and NA frequencies in petrol station workers were significantly higher than in control subjects (P < 0.01) and also significantly related to smoking habit (P < 0.01). Our findings indicate that the petrol station workers are under risk of significant cytogenetic damage.
authors.affiliation: Department of Biology, Faculty of Sciences and Letters, Mersin University, Ciftlikköy, 33342 Mersin, Turkey. a.celik@mersin.edu.tr
authors.forename: Ayla
authors.initials: A
authors.lastname: Celik
authors.forename: Tolga
authors.initials: T
authors.lastname: Cavaş
authors.forename: Serap
authors.initials: S
authors.lastname: Ergene-Gözükara
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D001554:Benzene / Q000009:adverse effects; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D020732:Cytogenetic Analysis; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects; D016273:Occupational Exposure; D010578:Petroleum / Q000009:adverse effects*; D019800:Phenol / Q000652:urine; D012907:Smoking
chemical_list: D000395:Air Pollutants, Occupational; D010578:Petroleum; D019800:Phenol; D001554:Benzene