Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells.
The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (DeltaPsim) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.
Miyoshi
Noriyuki
N
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan.
Nakamura
Yoshimasa
Y
Ueda
Yasuhiro
Y
Abe
Masako
M
Ozawa
Yoshio
Y
Uchida
Koji
K
Osawa
Toshihiko
T
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002396:Catechols; D003474:Curcumin / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism; D004032:Diet; D004224:Diterpenes / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D020939:Zingiber officinale / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D009153:Mutagens / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013141:Spiro Compounds / Q000494:pharmacology; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D002396:Catechols; D004224:Diterpenes; D005233:Fatty Alcohols; D009153:Mutagens; D009281:Naphthalenes; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013141:Spiro Compounds; D051028:bcl-2-Associated X Protein; C476264:galanal A; C476265:galanal B; C081562:8,17-epoxylabd-12-ene-15,16-dial; D045304:Cytochromes c; C007845:gingerol; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D003474:Curcumin