title: Oral epithelial cells are susceptible to cell-free and cell-associated HIV-1 infection in vitro.
abstract: Epithelial cells lining the oral cavity are exposed to HIV-1 through breast-feeding and oral-genital contact. Genital secretions and breast milk of HIV-1-infected subjects contain both cell-free and cell-associated virus. To determine if oral epithelial cells can be infected with HIV-1 we exposed gingival keratinocytes and adenoid epithelial cells to cell-free virus and HIV-1-infected peripheral blood mononuclear cells and monocytes. Using primary isolates we determined that gingival keratinocytes are susceptible to HIV-1 infection via cell-free CD4-independent infection only. R5 but not X4 viral strains were capable of infecting the keratinocytes. Further, infected cells were able to release infectious virus. In addition, primary epithelial cells isolated from adenoids were also susceptible to infection; both cell-free and cell-associated virus infected these cells. These data have potential implications in the transmission of HIV-1 in the oral cavity.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, 35294, USA. moorejs@uab.edu
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Moore
authors.forename: Firoz
authors.initials: F
authors.lastname: Rahemtulla
authors.forename: Leigh W
authors.initials: LW
authors.lastname: Kent
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Hall
authors.forename: Mine R
authors.initials: MR
authors.lastname: Ikizler
authors.forename: Peter F
authors.initials: PF
authors.lastname: Wright
authors.forename: Huan H
authors.initials: HH
authors.lastname: Nguyen
authors.forename: Susan
authors.initials: S
authors.lastname: Jackson
mesh_terms: D000234:Adenoids / Q000821:virology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D005881:Gingiva / Q000821:virology*; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000821:virology; D009061:Mouth Mucosa / Q000821:virology; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D015728:Receptors, HIV / Q000032:analysis / Q000378:metabolism; D014771:Virion / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D016655:HIV Core Protein p24; D015728:Receptors, HIV