Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 microm inside diameter silica microparticles by polar-organic mode capillary electrochromatography.
Different types of fused-silica capillaries of 75 microm inside diameter (ID) were packed, namely type A and B, and evaluated for the direct resolution of racemates of several basic compounds by enantioselective capillary electrochromatography (e-CEC). Type A was packed with a chiral stationary phase (CSP) containing teicoplanin (TE) mixed with silica microparticles (3:1 w/w) while type B contained only the TE-CSP. In both cases, particles of different sizes (3.5 and 5 microm ID) were employed. A polar-organic mobile phase containing methanol-acetonitrile (60-40% v/v and 0.05% w/v ammonium acetate was used. Several beta-blockers (alprenolol, oxprenolol, metoprolol, pindolol, salbutamol, propranolol, atenolol, acebutolol) were baseline-enantioresolved with both capillary types, in very short times.
Catarcini
Paolo
P
Istituto di Metodologie Chimiche, C.N.R., Monterotondo Scalo (Roma), Italy.
Fanali
Salvatore
S
Presutti
Carla
C
D'Acquarica
Ilaria
I
Gasparrini
Francesco
F
D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D010316:Particle Size; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D017334:Teicoplanin / Q000737:chemistry*
D000085:Acetates; D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists; D017334:Teicoplanin; D012822:Silicon Dioxide; C018824:ammonium acetate; D000432:Methanol; C032159:acetonitrile