Protein fouling in microfiltration: deposition mechanism as a function of pressure for different pH.
The influence of applied pressure on the fouling mechanism during bovine serum albumin (BSA) dead-end microfiltration (MF) has been investigated for a polyethersulfone acidic negatively charged membrane (ICE-450) from Pall Co. BSA solutions at pH values of 4, 5 (almost equal to the protein isoelectric point, IEP), and 6 were microfiltered through the membrane at different applied transmembrane pressures. Results have been analyzed in terms of the usual blocking filtration laws and a substantial change in the fouling mechanism was observed as the pressure was increased, this change can be related to the specific membrane-protein and protein-protein interactions.
Velasco
C
C
Departamento de Termodinámica y Fi;sica Aplicada, Facultad de Ciencias Universidad de Valladolid, 47071 Valladolid, Spain.
Ouammou
M
M
Calvo
J I
JI
Hernández
A
A
D000327:Adsorption; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016062:Porosity; D011312:Pressure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014462:Ultrafiltration / Q000379:methods*
D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine