Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test.
The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.
Sassone
Luciana Moura
LM
Faculty of Dentistry, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. lsassone@montreal.com.br
Fidel
Rivail Antonio Sergio
RA
Fidel
Sandra Rivera
SR
Dias
Marina
M
Hirata
Raphael Júnior
RJ
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002710:Chlorhexidine / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D012973:Sodium Hypochlorite / Q000008:administration & dosage*; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors
D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002710:Chlorhexidine