Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit.
Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is important in the human genome project. Here an automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed by using a homemade kit, which has lower cost but higher resolution than commercial kit. With this method, oncogene K-ras was investigated, four known SNPs of K-ras gene exon 1 in 31 coloerctal cancer patients were detected. Results indicate that mutations were present in 8(26%) of 31 patients, and most mutations were localized in codon 12. The presence of these mutations is thought to be a critical step and plays an important role in human colorectal carcinogenesisas.
Zhao
Chunxia
C
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, 116011 Dalian, China.
Xu
Guowang
G
Shi
Xianzhe
X
Ma
Jianmei
J
Zhang
Yan
Y
Lv
Shen
S
Yang
Qing
Q
D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D011905:Genes, ras; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers