Cloning, expression and characterization of a beta-agarase gene from a marine bacterium, Pseudomonas sp. SK38.
A gene (pagA) encoding beta-agarase from Pseudomonas sp. SK38 was cloned and expressed in Escherichia coli. The structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37326 and has a signal peptide of 18 amino acids. The deduced amino acid sequence showed 57% and 58% homology to beta-agarase from Pseudoalteromonas atalntica and Aeromonas sp., respectively. The recombinant enzyme was purified and biochemically characterized. The enzyme had maximum activity at pH 9 and 30 degrees C. It was stable at pHs from 8 to 9 and below 37 degrees C.
Kang
Nam-Young
NY
Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Choi
Yong-Lark
YL
Cho
Young-Su
YS
Kim
Beom-Kyu
BK
Jeon
Beong-Sam
BS
Cha
Jae-Young
JY
Kim
Cherol-Ho
CH
Lee
Young-Choon
YC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019624:agarase