Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni.
Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.
Verjovski-Almeida
Sergio
S
Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil. verjo@iq.usp.br
DeMarco
Ricardo
R
Martins
Elizabeth A L
EA
Guimarães
Pedro E M
PE
Ojopi
Elida P B
EP
Paquola
Apuã C M
AC
Piazza
João P
JP
Nishiyama
Milton Y
MY
Kitajima
João P
JP
Adamson
Rachel E
RE
Ashton
Peter D
PD
Bonaldo
Maria F
MF
Coulson
Patricia S
PS
Dillon
Gary P
GP
Farias
Leonardo P
LP
Gregorio
Sheila P
SP
Ho
Paulo L
PL
Leite
Ricardo A
RA
Malaquias
L Cosme C
LC
Marques
Regina C P
RC
Miyasato
Patricia A
PA
Nascimento
Ana L T O
AL
Ohlweiler
Fernanda P
FP
Reis
Eduardo M
EM
Ribeiro
Marcela A
MA
Sá
Renata G
RG
Stukart
Gaëlle C
GC
Soares
M Bento
MB
Gargioni
Cybele
C
Kawano
Toshie
T
Rodrigues
Vanderlei
V
Madeira
Alda M B N
AM
Wilson
R Alan
RA
Menck
Carlos F M
CF
Setubal
João C
JC
Leite
Luciana C C
LC
Dias-Neto
Emmanuel
E
D000818:Animals; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000469:parasitology; D014158:Transcription, Genetic
D015801:Helminth Proteins