Dependence of metastatic cancer cell invasion on MLCK-catalyzed phosphorylation of myosin regulatory light chain.
The role of myosin phosphorylation by myosin light chain kinase (MLCK) in regulating the invasiveness of metastatic cancer cells was investigated using the Dunning rat prostatic adenocarcinoma cell line, Mat Ly Lu, and in vitro invasion assay. Treatment with MLCK inhibitors resulted in marked reduction of invasiveness, which was principally due to impaired cellular motility, whereas the ability to survive and proliferate, to adhere to matrix, and to secrete gelatinases were minimally affected.
Tohtong
R
R
Department of Biochemistry, Faculty of Science, Mahidol University, Phya Thai, Bangkok, Thailand. scrth@mahidol.ac.th
Phattarasakul
K
K
Jiraviriyakul
A
A
Sutthiphongchai
T
T
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology*; D051381:Rats
D001381:Azepines; D018994:Myosin Light Chains; D009281:Naphthalenes; C056218:ML 9; C070571:ML 7; D009219:Myosin-Light-Chain Kinase; D008727:Methotrexate