Noncovalent attachment of psoralen derivatives with DNA: Hartree-Fock and density functional studies on the probes.
Two psoralen derivatives (probes) were prepared. Their geometries were optimized at the Hartree-Fock (HF) and Density Functional (B3LYP) levels employing 6-31G** and cc-pVDZ basis sets. Their interaction with DNA was investigated using spectrophotometric and computational techniques. Both of them have shown strong binding to calf thymus DNA. The red-shift and hypochromism that detected in the spectrum were taken as an evidence for the strong interaction between these probes and DNA. The spectrophotometric DNA titration data were treated by two different methodologies to calculate the intercalation affinity. Half-reciprocal plots gave binding constants of 5.5065 x 10(4) and 6.4727 x 10(4) for 8-butoxypsoralen (8-BOP) and 8-hexoxypsoralen (8-HOP), respectively. Schatchard plots gave a comparable intercalation binding constants and also the surface binding constants along with the number of intercalated probe molecules per base pair. The interaction between these probes and DNA were studied theoretically. The energy of interaction was computed using molecular mechanics method. Strength of interaction of these probes with different types of DNA was computed and compared. Calculated energies of interaction were compared with the observed intercalation affinities. HOMO and LUMO energies were computed and used to account for the strength of interaction.
El-Gogary
Tarek M
TM
Chemistry Department, Faculty of Science (Domyat), Mansoura University, Domyat AL-Gideda, Egypt. tarekelgogary@hotmail.com
El-Gendy
Eman M
EM
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005363:Ficusin / Q000378:metabolism*; D008958:Models, Molecular; D015335:Molecular Probes
D015335:Molecular Probes; D004247:DNA; D005363:Ficusin