Interindividual variability of phenol- and catechol-sulphotransferases in platelets from adults and newborns.
1 Phenol- and catechol- sulphotransferase activities were measured with p-nitrophenol and dopamine as substrates in platelets obtained from 100 newborns and 100 healthy adults. 2 Mean +/- (s.d.) estimates of catechol sulphotransferase activity were 7.07 +/- 5.93 (adult) and 13.3 +/- 6.42 (newborn) pmol min(-1) mg(-1) protein, respectively (P < 0.001). The coefficients of variation were 84% (adult) and 48% (newborn). The frequency distribution of sulphotransferase activity was symmetric and did not deviate significantly from normality in newborn platelets, but was positively skewed in adult platelets. 3 Mean +/- (s.d.) estimates of phenol sulphotransferase activity were 3.01 +/- 3.21 (adult) and 4.80 +/- 4.34 (newborn) pmol min(-1) mg(-1), respectively (P < 0.001). The coefficients of variation were 107% (adult) and 90% (newborn). The frequency distribution of sulphotransferase activity was positively skewed in both newborn and adult platelets. 4 Since sulphotransferase activity in platelets is well-expressed at birth and a prenatal development of sulphotransferase has been described in mid-gestational human foetal liver, the newborn should be able to sulphate drugs.
Pacifici
G M
GM
Department of Biomedicine, Medical School, Via Roma 55, I-56100 Pisa, Italy.
Marchi
G
G
D000328:Adult; D015239:Arylsulfotransferase / Q000737:chemistry / Q000378:metabolism*; D001792:Blood Platelets / Q000201:enzymology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male
D015239:Arylsulfotransferase