title: The structure of the extracellular region of human hepsin reveals a serine protease domain and a novel scavenger receptor cysteine-rich (SRCR) domain.
abstract: Hepsin is an integral membrane protein that may participate in cell growth and in maintaining proper cell morphology and is overexpressed in a number of primary tumors. We have determined the 1.75 A resolution structure of the extracellular component of human hepsin. This structure includes a 255-residue trypsin-like serine protease domain and a 109-residue region that forms a novel, poorly conserved, scavenger receptor cysteine-rich (SRCR) domain. The two domains are associated with each other through a single disulfide bond and an extensive network of noncovalent interactions. The structure suggests how the extracellular region of hepsin may be positioned with respect to the plasma membrane.
authors.affiliation: Celera, Inc., 180 Kimball Road, South San Francisco, CA 94080, USA. john.somoza@celera.com
authors.forename: John R
authors.initials: JR
authors.lastname: Somoza
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Ho
authors.forename: Christine
authors.initials: C
authors.lastname: Luong
authors.forename: Manjiri
authors.initials: M
authors.lastname: Ghate
authors.forename: Paul A
authors.initials: PA
authors.lastname: Sprengeler
authors.forename: Kyle
authors.initials: K
authors.lastname: Mortara
authors.forename: William D
authors.initials: WD
authors.lastname: Shrader
authors.forename: David
authors.initials: D
authors.lastname: Sperandio
authors.forename: Hedy
authors.initials: H
authors.lastname: Chan
authors.forename: Mary E
authors.initials: ME
authors.lastname: McGrath
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Katz
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry*; D051116:Receptors, Scavenger; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D012697:Serine Endopeptidases; C055645:hepsin