Constructing thromboresistant surface on biomedical stainless steel via layer-by-layer deposition anticoagulant.
Multilayer films consisting of polyethylenimine (PEI) and heparin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly (ESA) of the PEI and heparin. The process of ESA of PEI/heparin was monitored by static contact angle, electrochemical impedance spectroscopy (EIS), reflection adsorption spectroscopy and X-ray photoelectron spectroscopy data. The contact angle and EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. The static platelet adhesion and static clotting time experiments indicated that the PEI/heparin-deposited stainless steel could resist the platelet adhesion and prolong the static clotting time effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices.
Tan
Qinggang
Q
Department of Polymer Science, Zhejiang University, Hangzhou 310027, China.
Ji
Jian
J
Barbosa
M A
MA
Fonseca
Carlos
C
Shen
Jiacong
J
D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000502:physiology; D011094:Polyethyleneimine / Q000737:chemistry*; D013193:Stainless Steel / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D013193:Stainless Steel; D011094:Polyethyleneimine; D006493:Heparin