VEGF expression and its reguration by p53 gene transfection in endometrial carcinoma cells.
Vascular endothelial growth factor (VEGF) that activates endothelial cell growth induces angiogenesis, which is indispensable to tumor igenesis and tumor progression. On the other hand, tumor suppressor gene p53 has been considered to regulate VEGF expression, but the detailed relationship between them remains unclear. In this study, we aimed to study VEGF expression in endometrial carcinoma cells and the effect of p53 gene transfection on VEGF expression using p53-mutated endometrial carcinoma cell line, HEC-50B. Immunoblotting for detecting VEGF protein, p53 protein and beta-actin was performed using 11 endometrial carcinoma cell lines. Levels of VEGF in the cultured media were measured by Enzyme immunoassay(EIA). Transfection of wild p53 gene was carried out by SuperFect method in HEC-50B cells, which had mutant p53 gene and did not express p53 protein. The results of immunoblotting were analyzed by NIH image and expressed as values. The results of EIA were expressed as the relative value. The VEGF value was 0.8 +/- 0.3 (n = 6) in p53-wild group, whereas in p53-mutant group it was 1.6 +/- 0.8 (n = 5). VEGF expression was correlated significantly with p53 status (P < 0.05). VEGF levels in p53 gene-transfected cells and the conditioned medium were decreased in 48 hours after p53 gene transfection. VEGF expression was down-regulated by p53 in endometrial carcinoma cells.
Fujisawa
T
T
Department of Clinical Cytology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan.
Watanabe
J
J
Kamata
Y
Y
Hamano
M
M
Hata
H
H
Kuramoto
H
H
D045744:Cell Line, Tumor; D003470:Culture Media / Q000737:chemistry; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D003470:Culture Media; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A