Effect of dietary pectin on the production of immunoglobulins and cytokines by mesenteric lymph node lymphocytes in mouse colitis induced with dextran sulfate sodium.
The present study explores the dietary effect of pectin on the MLN lymphocyte functions of mice with dextran sulfate sodium (DS)-induced colitis. We found that the immunoglobulin (Ig)A level in mesenteric lymph node (MLN) lymphocytes was high, while the IgE level was lower, in mice fed with pectin than in those fed with cellulose. Interestingly, the fecal IgA concentration of the pectin-fed mice was significantly higher than that of the cellulose-fed mice. The concentrations of interferon-gamma and interleukin (IL)-2 treated with concanavalin A (ConA) were significantly higher in the pectin-fed group than in the cellulose-fed group. Although dietary pectin did not affect the IL-4 and IL-10 levels, the activation-induced IL-4 and IL-10 secretion was lower in MLN cells of the pectin-fed mice than of the cellulose-fed mice following DS-induced colitis. Based on these findings, we propose that the effect of dietary pectin on mice with DS-induced colitis is mediated by the manipulation of Th1 cells. Furthermore, the inhibitory effect of IL-4 and IL-10 by dietary pectin may play an important role in promoting a change in Th1/Th2 balance toward Th1-dominant immunity.
Lim
Beong Ou
BO
Graduate School of East-West Medical Science, Department of Medical Nutrition, Kyung Hee University, 1 Hoeki-Dong, Dongdaemoon-ku, Seoul 130-701, Korea. beongou@khu.ac.kr
Lee
Sun Hee
SH
Park
Dong Ki
DK
Choue
Ryo Won
RW
D000818:Animals; D001835:Body Weight; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D016264:Dextran Sulfate; D004043:Dietary Fiber / Q000494:pharmacology; D004435:Eating; D005243:Feces / Q000737:chemistry; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008643:Mesentery / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010368:Pectins / Q000494:pharmacology*; D013997:Time Factors
D016207:Cytokines; D004043:Dietary Fiber; D007070:Immunoglobulin A; D007073:Immunoglobulin E; D010368:Pectins; D016264:Dextran Sulfate