Laccase from a non-melanogenic, alkalotolerant gamma-proteobacterium JB isolated from industrial wastewater drained soil.
A gram-negative, alkalotolerant bacterium, isolated from the soil continually drained with industrial wastewater and identified as gamma-proteobacterium by partial 16S rRNA sequence analysis, produced a polyphenol oxidase, which showed laccase but not tyrosinase activity. The organism grew well from pH 6 to 10 and produced laccase maximally at pH 10. The enzyme was stable from pH 3 to 10.6 for at least 24 h and was optimally active at 55 degrees C and pH 6.5 in a 5 min assay.
Bains
Jasleen
J
Department of Microbiology, Panjab University, Chandigarh 160014, India.
Capalash
Neena
N
Sharma
Prince
P
D004269:DNA, Bacterial / Q000235:genetics; D020563:Gammaproteobacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D042845:Laccase / Q000096:biosynthesis* / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants; D013379:Substrate Specificity; D013696:Temperature; D014443:Tyrosine / Q000378:metabolism
D004269:DNA, Bacterial; D007220:Industrial Waste; D008543:Melanins; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D014443:Tyrosine; D042845:Laccase