BRAF and K-ras gene mutations in human pancreatic cancers.
We investigated the frequency of BRAF mutations in human pancreatic cancer specimens to determine its role in the development of pancreatic cancer. Nine pancreatic cancer samples without a K-ras codon 12 mutation and 19 with a K-ras mutation were included in the study. Analyses of the BRAF sequence revealed mutations in exon 15 (V599E) in two cases, both of which also exhibited a K-ras codon 12 mutation. No BRAF mutation was found in cases without a K-ras mutation. The BRAF V599E mutation was not found to be a major mutation in pancreatic cancers that had no K-ras codon 12 mutation.
Ishimura
Norihisa
N
Second Department of Internal Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo-shi, Shimane 693-8501, Japan.
Yamasawa
Kunihiro
K
Karim Rumi
Mohammad Azharul
MA
Kadowaki
Yasunori
Y
Ishihara
Shunji
S
Amano
Yuji
Y
Nio
Yoshinori
Y
Higami
Tetsuya
T
Kinoshita
Yoshikazu
Y
D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics; D002288:Adenocarcinoma, Mucinous / Q000209:etiology / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000209:etiology / Q000235:genetics; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D015513:Oncogene Proteins / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000209:etiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D048493:Proto-Oncogene Proteins B-raf; D015996:Survival Rate; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D015513:Oncogene Proteins; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf