title: Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples.
abstract: This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain. aguero@inia.es
authors.forename: M
authors.initials: M
authors.lastname: Agüero
authors.forename: J
authors.initials: J
authors.lastname: Fernández
authors.forename: L
authors.initials: L
authors.lastname: Romero
authors.forename: C
authors.initials: C
authors.lastname: Sánchez Mascaraque
authors.forename: M
authors.initials: M
authors.lastname: Arias
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000358:African Swine Fever Virus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral