title: Centrosome positioning in interphase cells.
abstract: The position of the centrosome is actively maintained at the cell center, but the mechanisms of the centering force remain largely unknown. It is known that centrosome positioning requires a radial array of cytoplasmic microtubules (MTs) that can exert pushing or pulling forces involving MT dynamics and the activity of cortical MT motors. It has also been suggested that actomyosin can play a direct or indirect role in this process. To examine the centering mechanisms, we introduced an imbalance of forces acting on the centrosome by local application of an inhibitor of MT assembly (nocodazole), and studied the resulting centrosome displacement. Using this approach in combination with microinjection of function-blocking probes, we found that a MT-dependent dynein pulling force plays a key role in the positioning of the centrosome at the cell center, and that other forces applied to the centrosomal MTs, including actomyosin contractility, can contribute to this process.
authors.affiliation: Department of Physiology and Center for Biomedical Imaging, University of Connecticut Health Center, Technology, Farmington, CT 06032-1507, USA.
authors.forename: Anton
authors.initials: A
authors.lastname: Burakov
authors.forename: Elena
authors.initials: E
authors.lastname: Nadezhdina
authors.forename: Boris
authors.initials: B
authors.lastname: Slepchenko
authors.forename: Vladimir
authors.initials: V
authors.lastname: Rodionov
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D018385:Centrosome / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004398:Dyneins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007399:Interphase / Q000235:genetics*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009218:Myosins / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D014404:Tubulin; D009218:Myosins; D004398:Dyneins; D020764:cdc42 GTP-Binding Protein; D015739:Nocodazole