Human cord blood-derived mast cells synthesize and release I-309 in response to IgE.
Mast cells are the central mediating cells of allergic reactions. Binding of allergen specific IgE to high affinity IgE receptor (Fcepsilon RI) and subsequent binding of allergen by the IgE causes receptor cross-linking and activation. In a study examining the differential gene expression in human cord blood-derived mast cells (CBMCs) mediated by activation of Fcepsilon RI both with IgE and IgE followed by cross-linking with alpha-IgE, the chemokine I-309 was found to be upregulated. I-309 is the ligand for the CCR8 receptor and is responsible for chemoattraction of TH2 type T-cells. Interestingly, I-309 RNA and protein levels were elevated not only in response to IgE/alpha-IgE activation but also by IgE alone. In addition, the I-309 levels were augmented by growth of the CBMCs in the presence of the proinflammatory cytokine IL-4. GM-CSF and MIP-1alpha secretion was also induced by IgE. These results suggest that IgE, through the production and release of cytokines such as I-309, GM-CSF and MIP-1alpha could promote an inflammatory reaction in the absence of antigen stimulation of mast cells.
Gilchrest
Helen
H
Schering-Plough Research Institute, 2015 Galloping Hill Rd. MS-1600, Kenilworth, NJ 07033, USA.
Cheewatrakoolpong
Boonlert
B
Billah
Motasim
M
Egan
Robert W
RW
Anthes
John C
JC
Greenfeder
Scott
S
D054402:Chemokine CCL1; D018925:Chemokines / Q000378:metabolism; D019742:Chemokines, CC / Q000378:metabolism*; D003432:Cross-Linking Reagents; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers; D005312:Fetal Blood / Q000166:cytology*; D015870:Gene Expression; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D008407:Mast Cells / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D054401:Receptors, CCR8; D019707:Receptors, Chemokine / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C065971:CCL1 protein, human; C103330:CCR8 protein, human; D054402:Chemokine CCL1; D018925:Chemokines; D019742:Chemokines, CC; D003432:Cross-Linking Reagents; D016207:Cytokines; D017931:DNA Primers; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine