Use of water-dispersible Fe(2)O(3) nanoparticles with narrow size distributions in isolating avidin.
Synthetic approaches that vigorously control the microstructures of water-dispersible gamma-Fe(2)O(3) nanoparticles such as size and size uniformity are of importance to the potential biological applications of these nanomaterials. In the present paper, water-dispersible gamma-Fe(2)O(3) nanocrystals with narrow size distributions (bipy-Fe(2)O(3)) were prepared via a site-exchange reaction. These particular materials are superparamagnetic and stable within a wide range of pH. Introduction of the biotin functionality onto the surfaces of bipy-Fe(2)O(3) enabled the affinity isolation of the protein avidin from its incubation solution magnetically with 96% efficiency.
Fan
Jinda
J
Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901-4409, USA.
Lu
Junge
J
Xu
Ruisong
R
Jiang
Rong
R
Gao
Yong
Y
D001360:Avidin / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D014867:Water / Q000737:chemistry*
D005290:Ferric Compounds; D014867:Water; D001360:Avidin; C000499:ferric oxide