title: The galactose-binding lectin from Vatairea macrocarpa seeds induces in vivo neutrophil migration by indirect mechanism.
abstract: To explore the pathways by which lectins induce an inflammatory response, the lectin from Vatairea macrocarpa (VML) seeds was used to induce neutrophil migration in rats. The lectin was shown to cause cell migration, with the effect partially blocked when galactose was added to inhibit lectin activity. Neutrophil migration was also reduced when peritoneal cavity of the animals was depleted of their resident cells beforehand, suggesting that neutrophil migration was mediated by an indirect mechanism. Pre-treatment of rats with thioglycollate increased recruitment of neutrophils while depletion of mast cells by the addition of compound 48/80 had little effect on neutrophil infiltration, suggesting the involvement of macrophages in the inflammatory process induced by the lectin. Inhibition of the cyclooxigenase, leukotriene and PAF activities by indomethacin, MK886 and BN50730, respectively, did not modify the pro-inflammatory effect previously observed. However, dexamethasone and thalidomide significantly reduced the population of neutrophils in the peritoneal cavity after lectin injection. The present study suggests that the effects produced by a galactose-binding lectin do not involve lipoxygenase, cyclooxygenase or PAF mediators that are well known to be involved in the inflammatory process. The blocking actions of dexamethasone and thalidimide suggest that as yet unidentified pro-inflammatory mediators are involved.
authors.affiliation: Departamentos de Fisiologia e Farmacologia, Universidade Federal do Ceará, 60.451-970 Ceará, Fortaleza, Brazil. nylanenunes@hotmail.com
authors.forename: Nylane M N
authors.initials: NM
authors.lastname: Alencar
authors.forename: Ana M S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Veruska B M
authors.initials: VB
authors.lastname: Alencar
authors.forename: Sabrina C
authors.initials: SC
authors.lastname: Melo
authors.forename: Marcio V
authors.initials: MV
authors.lastname: Ramos
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Fernando Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: Ronaldo A
authors.initials: RA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D037161:Galectins / Q000378:metabolism / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010533:Peritoneal Lavage; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013792:Thalidomide / Q000494:pharmacology; D013864:Thioglycolates / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D037161:Galectins; D005938:Glucocorticoids; D007166:Immunosuppressive Agents; D013864:Thioglycolates; D013792:Thalidomide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003907:Dexamethasone; D008358:Mannose; D005690:Galactose; D007213:Indomethacin