DRB1*1446 allele variations in the group-specific annealing sites of common amplification primers.
A cord blood unit from the Umbilical Cord Bank of Barcelona was initially typed by sequence-based typing (SBT) as DRB1* 0701. However, the subsequent confirmation by sequence-specific oligonucleotide probes (SSOP) revealed an unusual hybridization pattern that did not fit any of the known HLA-DRB1 alleles or allelic combinations. Molecular cloning and sequencing with primers located at introns 1 and 2 provided the definitive identification of a novel DRB1*1446 allele. It was similar to DRB1*1402 at exon 2 except for nucleotide substitutions at codons 10, 11 and 12 (from YST to LRK) that coincide with the 5'-end group specific site for the annealing of common amplification primers used by SBT and sequence-specific primers.
Morante
M P
MP
Department of Immunology, Hospital Sant Pau, Barcelona, Spain.
Mosquera
A M
AM
Querol
S
S
Garcia
J
J
Rodriguez-Sanchez
J L
JL
Vidal
S
S
D000483:Alleles; D017931:DNA Primers; D005312:Fetal Blood; D014644:Genetic Variation; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006579:Heterozygote; D006801:Humans; D015335:Molecular Probes
D017931:DNA Primers; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015335:Molecular Probes