title: Cold storage affects survival and growth of Erwinia amylovora on the calyx of apple.
abstract: OBJECTIVE
  To determine the effect of cold storage on the survival of Erwinia amylovora.
  
  
  RESULTS
  The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low temperatures. In contrast, populations of E. amylovora on tissue in the apple calyx decreased during cold storage.
  
  
  CONCLUSIONS
  Erwinia amylovora has the ability, in nutrient media, to multiply at low temperatures. However, populations of E. amylovora on tissue in the apple calyx decrease with the time spent in cold storage.
  
  
  CONCLUSIONS
  Cold storage of apples will provide assurance that mature fruit from orchards, free of fire blight, or even with low levels of fire blight, may be exported with a negligible risk of introducing the disease into countries free of fire blight.
authors.affiliation: The Horticulture and Food Research Institute of New Zealand Ltd, Auckland, New Zealand.
authors.forename: R K
authors.initials: RK
authors.lastname: Taylor
authors.forename: C N
authors.initials: CN
authors.lastname: Hale
mesh_terms: D003080:Cold Temperature; D015169:Colony Count, Microbial; D044084:Erwinia amylovora / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D005511:Food Handling / Q000379:methods*; D027845:Malus / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction