Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity.
A novel L-amino acid oxidase, named TSV-LAO, has been purified and cloned from the snake Trimeresurus stejnegeri. Fifty percentage cytotoxic concentrations (CC50) of TSV-LAO on C8166 cells were 24 and 390 nM in the absence or presence of catalase (400 nM), respectively. However, at concentrations that showed little effect on cell viability, TSV-LAO displayed dose dependent inhibition on HIV-1 infection and replication. The antiviral selectivity indexes (CC50/EC50) were 16 and 6, respectively, corresponding to the measurements of syncytium formation and HIV-1 p24 antigen expression. Interestingly, the presence of catalase resulted in an increase of its antiviral selectivity to 52 and 38. Under the same conditions, no anti-HIV-1 activity was observed by exogenous addition of H2O2. The complete amino acid sequence of TSV-LAO, as deduced from its cDNA, exhibits a high degree of sequence identity with other snake venom LAOs.
Zhang
Yu-Jie
YJ
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
Wang
Jian-Hua
JH
Lee
Wen-Hui
WH
Wang
Qian
Q
Liu
Heng
H
Zheng
Yong-Tang
YT
Zhang
Yun
Y
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D015497:HIV-1 / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D050830:L-Amino Acid Oxidase; D008969:Molecular Sequence Data; D014757:Viper Venoms / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity
D019380:Anti-HIV Agents; D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D014757:Viper Venoms; D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; C478161:TSV-LAO protein, Trimeresurus stejnegeri