Effect of corticosterone treatment on mitochondrial oxidative energy metabolism in developing rat brain.
Effects of "acute" and "chronic" corticosterone treatment on energy metabolism in brain mitochondria from rats belonging to various age groups were examined. Both acute and chronic treatments resulted in significant reduction in the state 3 respiration rates and in the lowering of ADP/O ratios with the various substrates tested. The effects were age and substrate specific and in general the extent of uncoupling was greater in chronically treated rats. Corticosterone treatments also resulted in site-specific uncoupling in an age-dependent manner. The overall effect of corticosterone treatments was lowering of ADP phosphorylation rates, thereby causing an energy deficit. The effects on dehydrogenase activities were of a variable nature. Our results suggest that energy deficits following corticosteroid treatment may be an underlying cause for impaired brain development reported by earlier researchers.
Katyare
S S
SS
Department of Biochemistry, Faculty of Science, M.S. University of Baroda, Vadodara, Gujarat 390 002, India. katyare_ss@hotmail.com
Balasubramanian
Sureshkumar
S
Parmar
D V
DV
D000244:Adenosine Diphosphate / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D003345:Corticosterone / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004734:Energy Metabolism / Q000187:drug effects*; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D010088:Oxidoreductases; D003345:Corticosterone