[Interaction between the metal ion in the active site of cytochrome C and copper sulfate by ultraviolet spectroscopy].
The direct interaction between the metal ion in the active center of cytochrome C (Fecyt-C) and inorganic copper sulfate in pH 7.1 phosphate has been studied by UV spectrometry. As a result, part of the metal ion Fe(II) in Fecyt-C was replaced by Cu(II) and the corresponding cytochrome C derivatives Cucyt-C were produced, in addition, the interaction was increased with amount of copper sulfate added. The direct interaction between the CuSO4 and metal ion of cytochrome C was verified for the first time.
Jia
Y
Y
Department of Chemistry and Chemical Engineering, Dalian University, 116622 Dalian.
Zheng
X
X
Hu
J
J
D002412:Cations; D019327:Copper Sulfate / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D004347:Drug Interactions; D005296:Ferrous Compounds / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D002412:Cations; D005296:Ferrous Compounds; D045304:Cytochromes c; D007501:Iron; D019327:Copper Sulfate