title: Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction.
abstract: The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA extracts of individual whiteflies (Bemisia tabaci) that had fed on TYLCV-infected plants. One of the characteristics of the LAMP method is its ability to synthesize an extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophosphate ion, is produced yielding a white precipitate of magnesium pyrophosphate in the reaction mixture. The presence or absence of this white precipitate allows easy detection of amplification of TYLCV genomic DNA without gel electrophoresis.
authors.affiliation: Aichi-ken Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi 480-1193, Japan. sfukuta@agri-rc.pref.aichi.jp
authors.forename: Shiro
authors.initials: S
authors.lastname: Fukuta
authors.forename: Shinro
authors.initials: S
authors.lastname: Kato
authors.forename: Keiko
authors.initials: K
authors.lastname: Yoshida
authors.forename: Yuko
authors.initials: Y
authors.lastname: Mizukami
authors.forename: Akira
authors.initials: A
authors.lastname: Ishida
authors.forename: Junnichi
authors.initials: J
authors.lastname: Ueda
authors.forename: Michio
authors.initials: M
authors.lastname: Kanbe
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ishimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D011232:Chemical Precipitation; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D017791:Geminiviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005784:Gene Amplification; D006430:Hemiptera / Q000502:physiology / Q000821:virology*; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010935:Plant Diseases / Q000821:virology; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral