Forced expression of cyclin D1 does not compensate for Id2 deficiency in the mammary gland.
Id2 and cyclin D1 share several biological activities, including inhibition of differentiation, stimulation of the G1-S transition in the cell cycle and stimulation of tumorigenesis. Mammary glands of Id2(-/-) mice display severely impaired lobulo-alveolar development during pregnancy, similarly to those of cyclin D1 null females. We investigated the functional relationship between Id2 and cyclin D1 in the mammary gland. Id2(-/-) mammary glands expressed a normal level of cyclin D1. No direct interaction of Id2 with cyclin D1 or its binding partner cdk4 was detected in mammalian two-hybrid assays. Ectopic expression of a cyclin D1 transgene did not rescue the mammary phenotype of Id2(-/-) mice. These results suggest that Id2 acts downstream or independently of cyclin D1 in the control of mammary cell proliferation during pregnancy.
Mori
Seiichi
S
Department of Biochemistry, Fukui Medical University, 23-3 Shimoaizuki, Matsuoka, 910-1193 Fukui, Japan.
Inoshima
Kenji
K
Shima
Yoko
Y
Schmidt
Emmett V
EV
Yokota
Yoshifumi
Y
D000818:Animals; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D051797:Inhibitor of Differentiation Protein 2; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D011247:Pregnancy; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D016213:Cyclins; D004268:DNA-Binding Proteins; C497452:Idb2 protein, mouse; D051797:Inhibitor of Differentiation Protein 2; D012097:Repressor Proteins; D014157:Transcription Factors; D019938:Cyclin D1