Activity of a flax pectin methylesterase promoter in transgenic tobacco pollen.
The regulatory region of the flax Lupme3 gene, which codes for a pectin methylesterase, contains two sequences (PB box) that are putative cis-active sequence elements thought to regulate transcription in pollen. The Lupme3 promoter was fused to the beta-glucuronidase (gus) reporter gene. The chimeric gene fusion was introduced into tobacco via Agrobacterium-mediated transformation. Expression of the reporter gene was monitored using a histochemical X-Gluc assay at different stages of pollen maturation and germination. The Lupme3 promoter was found to be active in germination-competent mature pollen and in pollen tube.
Lacoux
Jérôme
J
Laboratoire de Biotechnologies et Physiologie Végétales, Université de Picardie Jules Verne, Faculté des Sciences, 33, rue Saint-Leu, 80039 Amiens Cedex, France.
Duval
Isabelle
I
Dupré
Patricia
P
Gutierrez
Laurent
L
Lesueur
Sophie
S
Roger
David
D
Lainé
Eric
E
D001483:Base Sequence; D019597:Flax / Q000201:enzymology / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D011058:Pollen / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D053830:Plant Viral Movement Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins