Gene expression analysis of prostate hyperplasia in mice overexpressing the prolactin gene specifically in the prostate.
The probasin (Pb)-PRL transgenic mice that overexpress the rat PRL gene specifically in the prostate develop a dramatic enlargement of the prostate gland. The objective of this study was to characterize the molecular mechanisms involved in the prostate hyperplasia seen in the Pb-PRL transgenic mice. cDNA microarray analysis was used to identify differentially expressed transcripts in the hyperplastic prostates of 6-month-old transgenic mice compared with age-matched controls. We report the identification of 266 genes (175 up-regulated and 91 down-regulated) that were differentially expressed in the enlarged transgenic prostates compared with controls. Subsequential real-time RT-PCR was used to verify a set of differentially regulated transcripts. The hyperplastic prostates of Pb-PRL transgenic mice demonstrate a molecular pattern supporting the importance of reduced degree of apoptosis for the development of the phenotype. Immunohistochemical analysis of apoptotic activity using two different markers of apoptosis (single-stranded DNA and activated caspase-3) were performed, and the results showed diminished apoptosis activity in the prostate of Pb-PRL transgenic mice compared with control prostates. The increased stromal/epithelial ratio of the Pb-PRL transgenic prostate together with up-regulation of a significant fraction of genes involved in tissue remodeling activity, including the synthesis and degradation of the extracellular matrix and changes in protease activity, suggest that activation of the stroma is involved in the development of prostate hyperplasia. Overall, the differentially expressed transcripts identified in this study show many molecular similarities between the prostate hyperplasia of PRL-transgenic mice and human prostate pathology, including both benign prostatic hyperplasia and prostate cancer.
Dillner
Karin
K
Department of Pharmacology and Physiology, Göteborg University, 405 30, Sweden. karin.dillner@cmm.ki.se
Kindblom
Jon
J
Flores-Morales
Amilcar
A
Shao
Ruijin
R
Törnell
Jan
J
Norstedt
Gunnar
G
Wennbo
Håkan
H
D000727:Androgen-Binding Protein / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D003199:Computer Systems; D015536:Down-Regulation; D015870:Gene Expression; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D011388:Prolactin / Q000235:genetics*; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D000727:Androgen-Binding Protein; C027519:probasin; D011388:Prolactin