Protein kinase C/zeta (PRKCZ) gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes.
OBJECTIVE
To identify the susceptible gene (s) for type 2 diabetes in the previously mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.


METHODS
Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed.


RESULTS
Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/zetagene (PRKCZ) was statistically different between the case and control groups(P<0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.


CONCLUSIONS
PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.
Li
Yun-Feng
YF
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
Sun
Hong-Xia
HX
Wu
Guo-Dong
GD
Du
Wei-Nan
WN
Zuo
Jin
J
Shen
Yan
Y
Qiang
Bo-Qin
BQ
Yao
Zhi-Jian
ZJ
Wang
Heng
H
Huang
Wei
W
Chen
Zhu
Z
Xiong
Mo-Miao
MM
Meng
Yan
Y
Fang
Fu-De
FD
D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics*; D000099221:Protein Kinase C zeta
D011493:Protein Kinase C; D000099221:Protein Kinase C zeta