Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase.
Terminal deoxynucleotidyl transferase (TdT; EC 2.7.7.31) adds nucleotides to DNA ends generated during V(D)J recombination that are subsequently processed by proteins involved in general double-strand break (DSB) repair pathways. We report an association between TdT and a 55-kDa protein in lymphoid cells. This protein, identified as hPso4, is a homolog of the protein encoded by the PS04/PRP19 gene in Saccharomyces cerevisiae that has pleiotropic functions in DNA recombination and error-prone repair. Purified hPso4 binds double-stranded DNA in a sequence-nonspecific manner but does not bind single-stranded DNA. hPso4 protein is induced 15- to 30-fold in cells by gamma radiation and chemical mutagens but not by UV treatment. Loss of hPso4 expression induced by siRNA results in accumulation of DSBs, apoptosis, and decreased cell survival after DNA damage. We conclude that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair.
Mahajan
Kiran N
KN
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599-7295, USA. mahajan@med.unc.edu
Mitchell
Beverly S
BS
D000595:Amino Acid Sequence; D017209:Apoptosis; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival; D004249:DNA Damage; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D017931:DNA Primers; D004260:DNA Repair / Q000502:physiology*; D045643:DNA Repair Enzymes; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D034741:RNA, Small Interfering; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D002352:Carrier Proteins; D017931:DNA Primers; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D004253:DNA Nucleotidylexotransferase; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human