title: Female-specific DNA sequences in geese.
abstract: 1. The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung, Taiwan. mchuang@mail.nchu.edu.tw
authors.forename: M C
authors.initials: MC
authors.lastname: Huang
authors.forename: W C
authors.initials: WC
authors.lastname: Lin
authors.forename: Y M
authors.initials: YM
authors.lastname: Horng
authors.forename: R
authors.initials: R
authors.lastname: Rouvier
authors.forename: C W
authors.initials: CW
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D016172:DNA Fingerprinting / Q000662:veterinary; D017931:DNA Primers; D005260:Female; D005777:Geese / Q000235:genetics*; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*; D012737:Sex Factors
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA