[A new methodological approach for leptospira persistence studies in case of mixed leptospirosis].
A new methodical approach for Leptospira persistence studies in case of mixed leptospirosis, based on the use of PCR test systems with different taxonomic specificity for the indication and identification of leptospires, was developed. Two PCR test systems (G and B) were used in experiments on BALB/c white mice to study patterns of the development of mixed infection caused by leptospires of serovar poi (genomospecies L. borgpeterseni) and grippotyphosa (genomospecies L. kirschneri). The conclusion was made of good prospects of this method application in studies on symbiotic relationships of leptospires both in vivo and in vitro.
Samsonova
A P
AP
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Petrov
E M
EM
Vyshivkina
N V
NV
Anan'ina
Iu V
IuV
D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004195:Disease Models, Animal; D007668:Kidney / Q000382:microbiology; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007922:Leptospirosis / Q000175:diagnosis*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial
Novyĭ metodicheskiĭ pogkhod k izucheniiu leptospir pri smeshannoĭ leptospiroznoĭ infektsii.