Genome-wide location of yeast RNA polymerase III transcription machinery.
RNA polymerase III (Pol III) transcribes a large set of genes encoding small untranslated RNAs like tRNAs, 5S rRNA, U6 snRNA or RPR1 RNA. To get a global view of class III (Pol III-transcribed) genes, the distribution of essential components of Pol III, TFIIIC and TFIIIB was mapped across the yeast genome. During active growth, most class III genes and few additional loci were targeted by TFIIIC, TFIIIB and Pol III, indicating that they were transcriptionally active. SNR52, which encodes a snoRNA, was identified as a new class III gene. During the late growth phase, TFIIIC remained bound to most class III genes while the recruitment of Pol III and, to a lesser extent, of TFIIIB was down regulated. This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment.
Harismendy
Olivier
O
Service de Biochimie et de Génétique Moléculaire, Bâtiment 144, CEA/Saclay, 91191 Gif-sur-Yvette Cedex, France.
Gendrel
Christiane-Gabrielle
CG
Soularue
Pascal
P
Gidrol
Xavier
X
Sentenac
André
A
Werner
Michel
M
Lefebvre
Olivier
O
D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D012320:RNA Polymerase III / Q000235:genetics*; D012331:RNA, Fungal / Q000145:classification / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D019474:Transcription Factors, TFIII / Q000378:metabolism; D014158:Transcription, Genetic; D000098832:Transcription Factor TFIIIC
D012331:RNA, Fungal; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012320:RNA Polymerase III