Analysis of synthetic anti-diabetic drugs in adulterated traditional Chinese medicines by high-performance capillary electrophoresis.
Four synthetic anti-diabetic drugs, acetohexamide (ACE), chlorpropamide (CHL), glibenclamide (GLI) and tolbutamide (TOL), which can be found as adulterants in traditional Chinese medicines (TCMs) were assayed simultaneously using high-performance capillary electrophoresis (HPCE) in 4 min with UV detection at 200 nm. The electrolyte was a buffer solution containing 100 mM phosphate buffer (NaH2PO4/Na2B4O7, pH 7.5). Applied voltage was 15.0 kV and temperature was 30 degrees C. 2-(4-Hydroxyphenyl) ethyl ammonium chloride (HEA) was used as an internal standard. The effects of buffer concentration, pH and supplied voltage on separation were investigated. The relative standard deviations (R.S.D.) of these anti-diabetic drugs for intra-day and inter-day analyses were 0.23-4.27 and 1.23-6.33%, respectively. The recoveries of the synthetic drug adulterants in traditional Chinese medicinal formula ranged from 81.3 to 105.5%. GLI was found and determined in a real sample of TCM.
Ku
Yoe-Ray
YR
National laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2, Kuen-Yang Street, Nankang, Taipei, Taiwan. ayoe1344@nlfd.gov.tw
Chag
Li-Yun
LY
Ho
Li-Kang
LK
Lin
Jer-Huei
JH
D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000032:analysis*; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D012015:Reference Standards; D015203:Reproducibility of Results
D002021:Buffers; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions