[Extraction of glutethimide and methyprylon from blood using thin layer chromatography].
Glutethimide and methyprylone were extracted from blood and plasma at pH ca. 4 (acetate buffer) thrice with chloroform, using 2.5 solvent volume ratio to that of aqueous phase. Phenobarbital was used as an internal standard in thin-layer chromatography experiments on silica gel--to obtain of comparable ratios of Rf values of glutethimide and/or methyprylone to that of phenobarbital. Suitable conditions of the separation of three substances were established using one of following mobile phases: benzene-dioxane-aq.ammonia (15:4:1), chloroform-acetone-aq.ammonia (25:25:1), chloroform-acetone-diaethylamine (5:4:1) and chloroform-diethyl ether-acetone (5:3:2); on 7.5 x 2.5 cm plates; development time (at 5 cm distance) was up to 10 minutes. The concentrations as small as 0.1 microg of examined substances in 1 cm3 of plasma may be identified on chromatograms using 20% Na2CO3 solution.
Przyborowski
L
L
Katedra i Zakład Chemii Leków Akademii Medycznej w Lublinie, ul. Okopowa 1, 20-022 Lublin.
Zawisza
P
P
Hopkała
H
H
D002021:Buffers; D002855:Chromatography, Thin Layer / Q000379:methods*; D005984:Glutethimide / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010881:Piperidones / Q000097:blood* / Q000737:chemistry
D002021:Buffers; D010881:Piperidones; D005984:Glutethimide; C084583:methyprylon
Wykrywanie glutetimidu i metyprylonu we krwi metoda chromatografii cienkowarstwowej.