Evaluation of counterions for electrospray ionization mass spectral analysis of a highly sulfated carbohydrate, sucrose octasulfate.
A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other suffated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1 degree, 2 degrees, and 3 degrees ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.
Gunay
Nur Sibel
NS
Division of Medicinal and Natural Products Chemistry, Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA.
Tadano-Aritomi
Keiko
K
Toida
Toshihiko
T
Ishizuka
Ineo
I
Linhardt
Robert J
RJ
D002412:Cations; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000032:analysis*
D002412:Cations; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; C053081:sucrose octasulfate