Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine.
Chinese Meishan (MS) boars have smaller testes due to fewer Sertoli cells compared with White Composite (WC) boars. The objective was to describe Sertoli cell development relative to circulating FSH concentrations in fetal and neonatal MS and WC boars. Testes and blood samples were collected on days 60, 75, 90 and 105 postcoitum (dpc) and 1, 7, 14 and 25 postpartum (dpp). One testis was immunostained for GATA4 or Ki67 antigen to evaluate total and proliferating Sertoli cell numbers respectively. Testicular size was greater (P<0.01) in WC than MS boars at all ages, associated with a greater mass of interstitial tIssue. Tubular mass (P<0.01) was greater in prenatal WC boars, but postnatally increased more rapidly (P<0.001) in MS boars, exceeding WC boars by 25 dpp. Sertoli cell numbers increased with age, was greater (P<0.001) in WC than MS boars during prenatal development but increased rapidly (P<0.01) by 1 dpp in MS and thereafter was similar in both breeds. The proportion of Ki67-positive Sertoli cells was maximal at 90 dpc, declining thereafter, did not differ between breeds through 7 dpp, but was greater (P<0.05) in WC than MS boars at 14 and 25 dpp. Plasma FSH concentrations were greater (P<0.05) in WC than MS boars at 75 dpc. FSH concentrations were elevated at 105 dpc (MS) and 1 dpp (WC) but declined thereafter with advancing postnatal age in both breeds. This study illustrates that late gestation represents the period of maximal Sertoli cell proliferation. Despite asynchronous Sertoli cell population growth between breeds during early postnatal life, differential mature Sertoli cell numbers and testicular size are probably due to differences in duration of the proliferative period after 25 dpp, potentially regulated by Sertoli cell maturation and blood-testis barrier formation. These events were not associated with fetal or early postnatal changes in FSH secretion.
McCoard
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SA
United States Department of Agriculture, Agricultural Research Service, US Meat Animal Research Center, Clay Center, Nebraska 68933, USA. sue.mccoard@agresearch.co.nz
Wise
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TH
Lunstra
D D
DD
Ford
J J
JJ
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D002452:Cell Count; D002455:Cell Division; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005865:Gestational Age; D008297:Male; D012708:Sertoli Cells / Q000166:cytology*; D013045:Species Specificity; D013552:Swine / Q000033:anatomy & histology / Q000097:blood / Q000196:embryology*; D013737:Testis / Q000033:anatomy & histology / Q000196:embryology
D005640:Follicle Stimulating Hormone