Involvement of dopamine D3 and neuropeptide Y Y5 receptors in diabetic gastroparetic rats without response to erythromycin.
Erythromycin may accelerate gastric emptying in animals and human probably as an motilin agonist, but its prokinetic effects show obvious individual disparity. This study was to find the mechanism of this phenomenon. Microarray analysis was used to screen genes that might be involved in the response of diabetic gastroparesis rats to erythromycin. It was found that erythromycin accelerated gastric emptying of diabetic rats with great individual disparity. Through microarray analysis we screened differential expression genes that might be involved in the effect of erythromycin. Among 10 genes screened out, dopamine D3 receptor (DRD3) and neuropeptide Y Y5 receptor (NPYY5) genes were submitted to RT-PCR quantification and showed consistent results with microarray. It can be concluded that erythromycin promote gastric emptying of gastroparetic rats; DRD3 and NPYY5 may be involved in prokinetic action of erythromycin; and targets other than motilin receptor of erythromycin might exist as prokinetics.
Qin
Xin-Yu
XY
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China. xyqin@zshospital.com
Wang
Zhi-Gang
ZG
Fei
Jian
J
Liu
Feng-Lin
FL
Cui
Da-Fu
DF
Chen
Shao-Liang
SL
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D004917:Erythromycin / Q000494:pharmacology*; D005746:Gastric Emptying / Q000187:drug effects; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D018589:Gastroparesis / Q000209:etiology / Q000235:genetics / Q000517:prevention & control*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050637:Receptors, Dopamine D3; D017476:Receptors, Neuropeptide Y / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C493595:Drd3 protein, rat; D005765:Gastrointestinal Agents; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D017476:Receptors, Neuropeptide Y; C100568:neuropeptide Y5 receptor; D012313:RNA; D004917:Erythromycin