title: Probing the conformation of hemoglobin presbyterian in the R-state.
abstract: The influence of allosteric effectors on the R-state (liganded) conformation of Tg-HbP (human hemoglobin Presbyterian expressed in transgenic pig) has been probed using a number of biophysical techniques, and the results have been compared with that of liganded of HbA (human normal adult hemoglobin) to gain insight into the molecular basis of Asn-108(beta)->Lys mutation-induced low-oxygen affinity of Hb. The nuclear magnetic resonance studies of Tg-HbP revealed that the conformation of the alpha1beta1 and alpha1beta1 interfaces of the protein in the deoxy state are indistinguishable from that of deoxy HbA, whereas the conformation of the microenvironment of His-103(alpha) of Tg-HbP, a residue of the alpha1beta1 interface, is distinct from that of HbA in the R-state. In addition, the Presbyterian mutation also influences the structure of oxy Hb in other regions of the molecule. First, it facilitates the generation of deoxy (T)-state marker at 14.2 ppm (from 2,2-dimethyl-s-silapentane-5-sulfonate) on the interaction of oxy Hb with inositol hexa-phosphate without changing the ligation state. Second, it increases the geminate yield of the 10 ns photoproduct of CO-Hb. Third, it enhances the propensity of phosphate to increase the geminate yield. Fourth, it potentiates the ability of phosphate to induce deoxy-like features at the heme environment in the R-state. Fifth, it induces T-state-like signatures at the switch and hinge regions of the alpha1beta2 interface. Finally, molecular modeling studies have indicated an increased affinity for the four anion binding sites mapped in the midcentral cavity of Hb caused by the presence of Lys-108(beta). In short, Lys-108(beta) in HbP induces a propensity for oxy Hb to access T-like conformational features in different regions of the oxy Hb molecule and also enhances the T-like signatures in the oxy state on interaction with allosteric effectors without changing its ligation. Interestingly, the intrinsic T-like conformational features of the R-state of HbP, in addition to those induced by the addition of allosteric effectors to liganded HbP, appear to be reminiscent of features of the B-state conformation of Hb found in rHb 1.1 (recombinant hemoglobin). We propose that the lowered oxygen affinity of Tg-HbP in the presence of allosteric effectors is a consequence of an altered R-state conformation of Hb, which reflects the facilitation of switching the R-state of HbP to the T-state compared with the normal R-state of HbA, thereby reducing HbA's affinity to oxygen.
authors.affiliation: Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA. acharya@aecom.yu.edu
authors.forename: Seetharama A
authors.initials: SA
authors.lastname: Acharya
authors.forename: Ashok
authors.initials: A
authors.lastname: Malavalli
authors.forename: Eric
authors.initials: E
authors.lastname: Peterson
authors.forename: Philip D
authors.initials: PD
authors.lastname: Sun
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.forename: Muthuchidambaram
authors.initials: M
authors.lastname: Prabhakaran
authors.forename: Arthur
authors.initials: A
authors.lastname: Arnone
authors.forename: Belur N
authors.initials: BN
authors.lastname: Manjula
authors.forename: Joel M
authors.initials: JM
authors.lastname: Friedman
mesh_terms: D000494:Allosteric Regulation; D000495:Allosteric Site; D000818:Animals; D030801:Animals, Genetically Modified; D006441:Hemoglobin A / Q000737:chemistry / Q000378:metabolism; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009154:Mutation; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010777:Photochemistry; D020836:Protein Structure, Quaternary; D013059:Spectrum Analysis, Raman; D013552:Swine; D013696:Temperature
chemical_list: D006455:Hemoglobins, Abnormal; D007477:Ions; D008024:Ligands; C017361:hemoglobin Presbyterian; D006441:Hemoglobin A; D010100:Oxygen