Simultaneous determination of ticarcillin and clavulanate in rabbit serum and tissue cage fluid by liquid chromatography.
A gradient elution HPLC method with a wavelength switch technique was developed to simultaneously analyze the beta-lactam ticarcillin and the beta-lactamase inhibitor clavulanate in rabbit serum and tissue cage fluid (TCF). A C18 reversed-phase column with a programmable UV detector changing the wavelength from 218 to 254 nm at 9 min was used for chromatographic separation. The mobile phase consisted of acetonitrile, phosphate buffer and tetrabutylammonium hydrogen sulfate by following a gradient elution program at a flow-rate of 1 ml/min. Sample processing was carried out with liquid-liquid extraction. Good linearity, recoveries, precision and accuracy were obtained. The ranges of the standard curves were 1-100 microg/ml for ticarcillin, and 0.2-20 microg/ml for clavulanate. This assay has been successfully applied to analyze ticarcillin and clavulanate in rabbit serum and tissue cage fluid samples from a pharmacokinetic study.
Li
Chonghua
C
Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, USA.
Geng
Qiuming
Q
Nicolau
David P
DP
Nightingale
Charles H
CH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D019818:Clavulanic Acid / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013982:Ticarcillin / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D014018:Tissue Distribution
D000900:Anti-Bacterial Agents; D019818:Clavulanic Acid; D013982:Ticarcillin