The potential of site-specific recombinases as novel reporters in whole-cell biosensors of pollution.
DNA recombinases show some promise as reporters of pollutants providing that appropriate promoters are used and that the apparent dependence of expression on cell density can be solved. Further work is in progress using different recombinases and other promoters to optimize recombinase expression as well as to test these genetic constructs in contaminated environmental samples such as soil and water. It may be that a graded response reflecting pollutant concentration may not be possible. However, they show great promise for providing definitive detection systems for the presence of a pollutant and may be applicable to address the problem of bioavailability of pollutants in complex environments such as soil.
Hinde
Paul
P
School of Biological Sciences, University of Liverpool, Liverpool, L69 7ZB United Kingdom.
Meadows
Jane
J
Saunders
Jon
J
Edwards
Clive
C
D001419:Bacteria / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D017930:Genes, Reporter / Q000502:physiology; D045522:Recombinases / Q000378:metabolism*; D012988:Soil Microbiology; D015262:Xenobiotics / Q000378:metabolism*
D004785:Environmental Pollutants; D045522:Recombinases; D015262:Xenobiotics