Aberrations of p16INK4A, p14ARF and p15INK4B genes in pediatric solid tumors.
Expression of the p16INK4A (p16), p15INK4B (p15), and p14ARF genes, located at 9p21, was examined in pediatric neuroblastoma (NB), Ewing's sarcoma (ES), and rhabdomyosarcoma (RMS). p16 expression was absent in 4 of 5 ESs, and 2 of these 4 cases died. p16 expression was reduced or absent in 10 of 12 RMSs, and 4 of these 10 cases died. These results suggested the possibility that p16 expression was associated with the progression of ES and RMS. There has been no previous report on p14ARF in NB. Our investigation might indicate that abnormal expression of the p16 and p14ARF was associated with a poor prognosis in NB, although in some cases of NB normal p16 and abnormal p14ARF expression was seen. These findings suggest an important role of p14ARF gene in the tumorigenesis of NB. The different incidence of expression of the p16, p15, and p14ARF genes in these 3 tumor types may reflect differences of the molecular process through which the 3 tumors develop. Our results suggest that abnormal expression of the p16 and/or p14ARF may be associated with a poor prognosis in these 3 tumors.
Obana
Kazuko
K
Department of Pediatric Surgery, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
Yang
Hong-Wei
HW
Piao
Hui-Ying
HY
Taki
Tomohiko
T
Hashizume
Kohei
K
Hanada
Ryoji
R
Yamamoto
Keiko
K
Tanaka
Yukichi
Y
Toyoda
Yasunori
Y
Takita
Junko
J
Tsuchida
Yoshiaki
Y
Hayashi
Yasuhide
Y
D015139:Blotting, Southern; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D017353:Gene Deletion; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics / Q000401:mortality; D011379:Prognosis; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000401:mortality; D012512:Sarcoma, Ewing / Q000235:genetics / Q000401:mortality; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D025803:Tumor Suppressor Protein p14ARF; D025521:Tumor Suppressor Proteins; D012313:RNA; D004247:DNA