title: Age-dependent deficiency in import of mitochondrial DNA glycosylases required for repair of oxidatively damaged bases.
abstract: The mitochondria are the major source of chronic oxidative stress, which has been implicated in the aging process. Along with other cellular changes, aged cells accumulate mutations in both their nuclear and mitochondrial genomes, and they contain increased amounts of oxidatively damaged mutagenic bases such as 7,8-dihydro-8-oxoguanine, suggesting age-dependent inhibition of its repair. Surprisingly, the level and activity of 8-oxoguanine-DNA glycosylase (OGG1), responsible for repair of 7,8-dihydro-8-oxoguanine, was found to be higher in the liver mitochondrial extract from old rodents than in that from young ones. We addressed this paradox by analyzing OGG1 in the mitochondria of young vs. old mouse livers, as well as in replicating vs. presenescent human fibroblasts. We show here that although the total OGG1 activity is higher in old mitochondria, a large fraction of the enzyme is stuck to the membrane in the precursor form, which could not be translocated to and processed in the mitochondrial matrix. A nearly identical phenomenon was observed with the mitochondrial uracil-DNA glycosylase responsible for repair of mutagenic uracil. These results indicate an age-dependent decline in the mitochondrial import of proteins needed for DNA repair and possibly for other functions.
authors.affiliation: Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Szczesny
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Hazra
authors.forename: John
authors.initials: J
authors.lastname: Papaconstantinou
authors.forename: Sankar
authors.initials: S
authors.lastname: Mitra
authors.forename: Istvan
authors.initials: I
authors.lastname: Boldogh
mesh_terms: D001483:Base Sequence; D016922:Cellular Senescence; D004249:DNA Damage; D045647:DNA Glycosylases; D017931:DNA Primers; D004260:DNA Repair; D043382:DNA-Formamidopyrimidine Glycosylase; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000378:metabolism; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D018384:Oxidative Stress; D021381:Protein Transport; D014357:Trypsin / Q000378:metabolism
chemical_list: D017931:DNA Primers; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D014357:Trypsin