title: [The morphological and immunological characteristics of less-differentiated acute myeloid leukemic cells].
abstract: The aim was to study the morphological, histochemical and immunological characteristics of less-differentiated acute myeloid leukemic cells, and their diagnostic significance. Wright-Giemsa and histochemical staining were used to stain bone marrow smears from 2 case of AML-Mo. Immunological phenotypes were determined with flow cytometry. The results showed that myeloperoxidase stainings of both cases were negative, PAS was positive with fine particles, CD33/CD13 were positive, CD2/CD3/CD10/CD19/CD22 were negative. It is concluded that morphology, histochemistry and immunological phenotype on bone marrow smears are the main diagnostic basis for AML-Mo. The use of multiple monoclonal antibodies for staining may improve the accuracy.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China. cq2@21cn.com
authors.forename: Yi-Yan
authors.initials: YY
authors.lastname: Lai
authors.forename: Huo
authors.initials: H
authors.lastname: Tan
authors.forename: Xu
authors.initials: X
authors.lastname: Ye
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Lin-Juan
authors.initials: LJ
authors.lastname: Zeng
mesh_terms: D002454:Cell Differentiation; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged