Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini(Opisthorchidae, Trematoda).
The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.
Choi
Min-Ho
MH
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
Ryu
Jin-Sook
JS
Lee
Mejeong
M
Li
Shunyu
S
Chung
Byung-Suk
BS
Chai
Jong-Yil
JY
Sithithaworn
Paiboon
P
Tesana
Smarn
S
Hong
Sung-Tae
ST
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D003003:Clonorchiasis / Q000175:diagnosis*; D003004:Clonorchis sinensis / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006801:Humans; D015151:Immunoblotting / Q000662:veterinary; D008970:Molecular Weight; D009889:Opisthorchiasis / Q000175:diagnosis*; D009891:Opisthorchis / Q000276:immunology*
D000947:Antigens, Helminth