title: Predictive thermal inactivation model for effects of temperature, sodium lactate, NaCl, and sodium pyrophosphate on Salmonella serotypes in ground beef.
abstract: Analyses of survival data of a mixture of Salmonella spp. at fixed temperatures between 55 degrees C (131 degrees F) and 71.1 degrees C (160 degrees F) in ground beef matrices containing concentrations of salt between 0 and 4.5%, concentrations of sodium pyrophosphate (SPP) between 0 and 0.5%, and concentrations of sodium lactate (NaL) between 0 and 4.5% indicated that heat resistance of Salmonella increases with increasing levels of SPP and salt, except that, for salt, for larger lethalities close to 6.5, the effect of salt was evident only at low temperatures (<64 degrees C). NaL did not seem to affect the heat resistance of Salmonella as much as the effects induced by the other variables studied. An omnibus model for predicting the lethality for given times and temperatures for ground beef matrices within the range studied was developed that reflects the convex survival curves that were observed. However, the standard errors of the predicted lethalities from this models are large, so consequently, a model, specific for predicting the times needed to obtained a lethality of 6.5 log(10), was developed, using estimated results of times derived from the individual survival curves. For the latter model, the coefficient of variation (CV) of predicted times range from about 6 to 25%. For example, at 60 degrees C, when increasing the concentration of salt from 0 to 4.5%, and assuming that the concentration of SPP is 0%, the time to reach a 6.5-log(10) relative reduction is predicted to increase from 20 min (CV = 11%) to 48 min (CV = 15%), a 2.4 factor (CV = 19%). At 71.1 degrees C (160 degrees F) the model predicts that more than 0.5 min is needed to achieve a 6.5-log(10) relative reduction.
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA. vjuneja@arserrc.gov
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Juneja
authors.forename: Harry M
authors.initials: HM
authors.lastname: Marks
authors.forename: Tim
authors.initials: T
authors.lastname: Mohr
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D011756:Diphosphates / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology*; D008954:Models, Biological; D011200:Poultry; D012044:Regression Analysis; D012475:Salmonella / Q000145:classification* / Q000254:growth & development*; D012703:Serotyping / Q000379:methods; D012965:Sodium Chloride / Q000494:pharmacology*; D019354:Sodium Lactate / Q000494:pharmacology*; D013552:Swine; D013696:Temperature; D013816:Thermodynamics
chemical_list: D011756:Diphosphates; D012965:Sodium Chloride; C003319:sodium pyrophosphate; D019354:Sodium Lactate