title: A first-order-like state transition for recombinant protein folding.
abstract: Normally, proteins will aggregate and precipitate by direct folding processes. In this study, we report that quasi-static processes can restore both the structure and bio-function of two kinds of fish recombinant growth hormones (Plecoglossus altivelis and Epinephelus awoara). The conformational changes and the particle-size-distribution (PSD) of each refolding intermediate can be monitored by circular dichroism spectroscopy (CD) and dynamic light scattering (DLS), respectively. Conformation analysis of the CD spectra of the refolding intermediates indicated that the secondary structures were restored in the initial refolding intermediate. However, the tertiary interactions of the proteins were restored during the last two refolding stages, as elucidated by thermal stability tests. This is consistent with a sequential model. DLS analysis suggested that the average hydrodynamic radii of the refolding intermediates shrank to their native-like sizes after the first refolding stage. This is consistent with a collapse model. After comparison with the data on the direct folding process, it is concluded that the denaturant-containing protein folding reaction is a first-order-like state transition process.
authors.affiliation: Department of Physics, National Dong Hwa University,Hualien, Taiwan 97401. chiaching@mail.ndhu.edu.tw
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Chang
authors.forename: Ming-Sung
authors.initials: MS
authors.lastname: Cheng
authors.forename: Ya-Chi
authors.initials: YC
authors.lastname: Su
authors.forename: Lou-Sing
authors.initials: LS
authors.lastname: Kan
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D005399:Fishes; D013006:Growth Hormone / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D013816:Thermodynamics
chemical_list: D011994:Recombinant Proteins; D013006:Growth Hormone