[Influence of hypoxia-reoxygenation on nitric oxide synthesis III gene expression in cultured cerebral arterial endothelial cell].
OBJECTIVE
To investigate the change of nitric oxide synthase (NOS) III gene expression in cultured cerebral arterial endothelial cells during hypoxia and reoxygenation.


METHODS
(1) The cells were divided into six groups: control, hypoxia for 1 hour, reoxygenation for 2, 6, 12, 24 hours after hypoxia for 1 hour. (2) The expression of NOSIII mRNA was detected semiquantitatively by reverse transcription-polymerase chain reaction (RT-PCR). (3) Immunocytochemistry was used to detect the expression of NOSIII protein.


RESULTS
(1) The gene and protein expression of NOSIII was increased during hypoxia for 1 hour. (2) The gene and protein expression of NOSIII was decreased during reoxygenation for 2, 6, 12 hours after hypoxia for 1 hour, especially at 6 hours after reoxygenation. After cells were reoxygenation for 24 hours, the expression was restored to the normal level.


CONCLUSIONS
The experiment showed that hypoxia could increase the levels of NOSIII gene and protein expression and reoxygenation inhibited the increment of this gene expression.
Song
Zhen-ju
ZJ
Department of Emergency Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Yang
Guang-tian
GT
Lu
De-qin
DQ
Wang
Di-xun
DX
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D002536:Cerebral Arteries / Q000166:cytology / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D015870:Gene Expression; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine
D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III