ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera.
An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 x His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.
Ahn
Hye-Jin
HJ
Department of Parasitology and Catholic Institute of Parasitic Diseases, Catholic University of Korea, College of Medicine, Seoul 137-701, Korea.
Kim
Sera
S
Kim
Dae-Yong
DY
Nam
Ho-Woo
HW
D000818:Animals; D000913:Antibodies, Protozoan; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology; D003048:Coccidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000032:analysis*; D018688:Neospora / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016036:Seroepidemiologic Studies
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C110716:p43 protein, Neospora caninum