Antinociceptive profiles of crude extract from roots of Angelica gigas NAKAI in various pain models.
To characterize the antinociceptive profiles of Angelica gigas NAKAI (ANG; Korean angelica), methanol extract from the dried roots of ANG was made and mice were administered orally at the various doses (from 0.25 to 3 g/kg). ANG produced the increased latencies of the tail-flick and hot-plate paw-licking responses in a dose-dependent manner. In acetic acid-induced writhing test, ANG dose-dependently decreased writhing numbers. Moreover, the cumulative response time of nociceptive behaviors induced by intraplantar formalin injection was reduced during both the 1st and the 2nd phases in a dose-dependent manner in ANG-treated mice. Furthermore, oral administration of ANG did not cause licking, scratching and biting responses induced by TNF-alpha (100 pg), IFN-gamma (100 pg) or IL-1beta (100 pg) injected intrathecally (i.t.), especially at higher dose (3 g/kg). Additionally, in ANG treated mice, the cumulative nociceptive response time for i.t. administration of substance P or capsaicin was dose-dependently diminished. Finally, nociceptive responses elicited by i.t. injection of glutamate (20 microg), N-methyl-D-aspartic acid (60 ng), alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (13 ng) or kainic acid (12 ng) were decreased by oral administration of ANG. Our results suggest that ANG produces antinociception via acting on the central nervous system and shows antinociceptive profiles in various pain models, especially inflammatory pain.
Choi
Seong-Soo
SS
Department of Pharmacology, College of Medicine, Hallym University, Chunchon, Kangwon Do, South Korea.
Han
Ki-Jung
KJ
Lee
Han-Kyu
HK
Han
Eun-Jung
EJ
Suh
Hong-Won
HW
D000284:Administration, Oral; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D018846:Excitatory Amino Acids / Q000494:pharmacology; D005557:Formaldehyde; D006358:Hot Temperature; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010147:Pain Measurement / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D004856:Postural Balance / Q000187:drug effects; D011930:Reaction Time / Q000187:drug effects; D013373:Substance P / Q000494:pharmacology
D000700:Analgesics; D016207:Cytokines; D018846:Excitatory Amino Acids; D010936:Plant Extracts; D005557:Formaldehyde; D013373:Substance P; D002211:Capsaicin