title: A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction.
abstract: OBJECTIVE
  To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.
  
  
  METHODS
  Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.
  
  
  METHODS
  Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.
  
  
  METHODS
  Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).
  
  
  RESULTS
  No statistical differences were observed in any of the parameters investigated.
  
  
  CONCLUSIONS
  SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).
authors.affiliation: Fertility Clinic, Odense University Hospital, Copenhagen, Denmark. slaursen@health.sdu.dk
authors.forename: Steen B
authors.initials: SB
authors.lastname: Laursen
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
authors.forename: Johnny
authors.initials: J
authors.lastname: Hindkjaer
authors.forename: Karin
authors.initials: K
authors.lastname: Erb
mesh_terms: D000328:Adult; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods* / Q000592:standards*; D003718:Denmark; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011247:Pregnancy; D012099:Reproductive Techniques; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa