Biosynthetic origin of a branched chain analogue of the lipase inhibitor, lipstatin.
The lipophilic beta-lactone, lipstatin, inhibits pancreatic lipase and has been shown earlier to be biosynthesized by Claisen condensation of two fatty acid moieties. We present data from incorporation experiments with [U-13C6]leucine showing that a branched chain analogue of lipstatin is biosynthesized from leucine.
Eisenreich
Wolfgang
W
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Federal Republic of Germany.
Kupfer
Ernst
E
Stohler
Peter
P
Weber
Wolfgang
W
Bacher
Adelbert
A
D002247:Carbon Isotopes; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D007783:Lactones / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D007930:Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D008049:Lipase / Q000037:antagonists & inhibitors*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013302:Streptomyces / Q000378:metabolism
D002247:Carbon Isotopes; D004791:Enzyme Inhibitors; D007783:Lactones; C054164:lipstatin; D008049:Lipase; D007930:Leucine