Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability.
Mg2+ at 3.5 mM increased the tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae to ethanol. After 9 h of exposure to 20% (v/v) ethanol at 30 degrees C, all cells died whereas over 50% remained viable for the cells grown with Mg2+. The effect of Mg2+ is closely related to its ability to decrease plasma membrane permeability of cells subjected to ethanol stress.
Hu
Chun-Keng
CK
Department of Biological Engineering, Dalian University of Technology, 116024 Dalian, P.R. China.
Bai
Feng-Wu
FW
An
Li-Jia
LJ
D018533:Biomass; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D005285:Fermentation; D005427:Flocculation; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008560:Membrane Fluidity; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000502:physiology*
D000431:Ethanol; D008278:Magnesium Sulfate