ADAM33 is not associated with asthma in Puerto Rican or Mexican populations.
A recent study identified the ADAM33 gene as a promising candidate contributing to asthma. In Puerto Rican and Mexican populations, we have genotyped six single nucleotide polymorphisms (SNPs) that were used in the Genetics of Asthma in Latino Americans Study. We chose to study these two populations because in the United States, Puerto Ricans have the highest asthma prevalence, morbidity, and mortality and Mexicans the lowest. We used the transmission disequilibrium test to analyze associations between the ADAM33 gene variants and asthma, asthma severity, bronchodilator responsiveness, and total IgE levels using single SNPs, two to six SNP combinations, and specific haplotypes in 583 trios (proband with asthma and both biological parents). We also genotyped matched control samples to allow case-control analyses. None of the transmission disequilibrium test or case-control results showed significant association in either population. We found no evidence for association of single SNPs with asthma severity, bronchodilator response, or IgE levels in Mexicans or in the combined population. Two SNPs showed a modest association in Puerto Ricans, insignificant when the number of comparisons was taken into account. We conclude that the ADAM33 gene is not an important risk factor for asthma or for asthma-associated phenotypes in Mexicans or in Puerto Ricans.
Lind
Denise L
DL
University of California at San Francisco, San Francisco, CA 94143-0833, USA.
Choudhry
Shweta
S
Ung
Ngim
N
Ziv
Elad
E
Avila
Pedro C
PC
Salari
Keyan
K
Ha
Connie
C
Lovins
Elizabeth G
EG
Coyle
Natasha E
NE
Nazario
Sylvette
S
Casal
Jesus
J
Torres
Alfonso
A
Rodriguez-Santana
Jose R
JR
Matallana
Henry
H
Lilly
Craig M
CM
Salas
Jorge
J
Selman
Moises
M
Boushey
Homer A
HA
Weiss
Scott T
ST
Chapela
Rocio
R
Ford
Jean G
JG
Rodriguez-Cintron
William
W
Silverman
Edwin K
EK
Sheppard
Dean
D
Kwok
Pui-Yan
PY
González Burchard
Esteban
E
D051722:ADAM Proteins; D000293:Adolescent; D001249:Asthma / Q000097:blood / Q000188:drug therapy / Q000235:genetics*; D001993:Bronchodilator Agents / Q000008:administration & dosage; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005838:Genotype; D006630:Hispanic or Latino / Q000235:genetics*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics*; D017058:Mexican Americans / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012720:Severity of Illness Index
D001993:Bronchodilator Agents; D007073:Immunoglobulin E; D051722:ADAM Proteins; C450441:ADAM33 protein, human; D008666:Metalloendopeptidases