Direct and fast capillary zone electrophoretic method for the determination of Gleevec and its main metabolite in human urine.
A capillary zone electrophoretic (CZE) method was investigated for the determination of Gleevec and its main metabolite (N-demethylated piperazine derivative) in human urine using a fused-silica capillary (75 microm I.D.x60 cm total length, 10 cm effective length). The separation was performed with an hydrodynamic injection time of 10 s (0.5 p.s.i.) a voltage of -25 kV, a capillary temperature of 25 degrees C and a 100 mM phosphoric acid adjusted to pH 2 with the addition of triethanolamine. Under these conditions, the analysis takes about 5 min. A linear response over the 0.4-30.0 mg l(-1) concentration range was investigated for two compounds. A dilution of the sample was the only step necessary before the electrophoresis analysis. Detection limits of 0.1 mg l(-1) for Gleevec and its metabolite (S/N=3) were obtained. The developed method is easy, rapid and sensitive and has been applied to determine Gleevec and its main metabolite in clinical urine samples.
Rodríguez Flores
J
J
Facultad de Químicas, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain. juana.rflores@uclm.es
Berzas
J J
JJ
Castañeda
G
G
Rodríguez
N
N
D000970:Antineoplastic Agents / Q000652:urine*; D001549:Benzamides; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000652:urine*; D011743:Pyrimidines / Q000652:urine*; D015203:Reproducibility of Results; D013696:Temperature
D000970:Antineoplastic Agents; D001549:Benzamides; D002021:Buffers; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate