title: Use of water-dispersible Fe(2)O(3) nanoparticles with narrow size distributions in isolating avidin.
abstract: Synthetic approaches that vigorously control the microstructures of water-dispersible gamma-Fe(2)O(3) nanoparticles such as size and size uniformity are of importance to the potential biological applications of these nanomaterials. In the present paper, water-dispersible gamma-Fe(2)O(3) nanocrystals with narrow size distributions (bipy-Fe(2)O(3)) were prepared via a site-exchange reaction. These particular materials are superparamagnetic and stable within a wide range of pH. Introduction of the biotin functionality onto the surfaces of bipy-Fe(2)O(3) enabled the affinity isolation of the protein avidin from its incubation solution magnetically with 96% efficiency.
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901-4409, USA.
authors.forename: Jinda
authors.initials: J
authors.lastname: Fan
authors.forename: Junge
authors.initials: J
authors.lastname: Lu
authors.forename: Ruisong
authors.initials: R
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
mesh_terms: D001360:Avidin / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D014867:Water / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D014867:Water; D001360:Avidin; C000499:ferric oxide