title: Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni.
abstract: Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.
authors.affiliation: Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil. verjo@iq.usp.br
authors.forename: Sergio
authors.initials: S
authors.lastname: Verjovski-Almeida
authors.forename: Ricardo
authors.initials: R
authors.lastname: DeMarco
authors.forename: Elizabeth A L
authors.initials: EA
authors.lastname: Martins
authors.forename: Pedro E M
authors.initials: PE
authors.lastname: Guimarães
authors.forename: Elida P B
authors.initials: EP
authors.lastname: Ojopi
authors.forename: Apuã C M
authors.initials: AC
authors.lastname: Paquola
authors.forename: João P
authors.initials: JP
authors.lastname: Piazza
authors.forename: Milton Y
authors.initials: MY
authors.lastname: Nishiyama
authors.forename: João P
authors.initials: JP
authors.lastname: Kitajima
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Adamson
authors.forename: Peter D
authors.initials: PD
authors.lastname: Ashton
authors.forename: Maria F
authors.initials: MF
authors.lastname: Bonaldo
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Coulson
authors.forename: Gary P
authors.initials: GP
authors.lastname: Dillon
authors.forename: Leonardo P
authors.initials: LP
authors.lastname: Farias
authors.forename: Sheila P
authors.initials: SP
authors.lastname: Gregorio
authors.forename: Paulo L
authors.initials: PL
authors.lastname: Ho
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Leite
authors.forename: L Cosme C
authors.initials: LC
authors.lastname: Malaquias
authors.forename: Regina C P
authors.initials: RC
authors.lastname: Marques
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Miyasato
authors.forename: Ana L T O
authors.initials: AL
authors.lastname: Nascimento
authors.forename: Fernanda P
authors.initials: FP
authors.lastname: Ohlweiler
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Reis
authors.forename: Marcela A
authors.initials: MA
authors.lastname: Ribeiro
authors.forename: Renata G
authors.initials: RG
authors.lastname: Sá
authors.forename: Gaëlle C
authors.initials: GC
authors.lastname: Stukart
authors.forename: M Bento
authors.initials: MB
authors.lastname: Soares
authors.forename: Cybele
authors.initials: C
authors.lastname: Gargioni
authors.forename: Toshie
authors.initials: T
authors.lastname: Kawano
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Alda M B N
authors.initials: AM
authors.lastname: Madeira
authors.forename: R Alan
authors.initials: RA
authors.lastname: Wilson
authors.forename: Carlos F M
authors.initials: CF
authors.lastname: Menck
authors.forename: João C
authors.initials: JC
authors.lastname: Setubal
authors.forename: Luciana C C
authors.initials: LC
authors.lastname: Leite
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Dias-Neto
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000469:parasitology; D014158:Transcription, Genetic
chemical_list: D015801:Helminth Proteins