title: [Protective effect of follicle stimulating hormone on apoptosis of human epithelial ovarian cancer cell induced by cisplatin].
abstract: OBJECTIVE
  To observe the protective effects of follicle stimulating hormone (FSH) on human epithelial ovarian cancer cell apoptosis induced by cisplatin (DDP).
  
  
  METHODS
  OVCAR3-FSHR cell were treated with DDP and FSH at serials of concentrations, MTT assay was used to examine the growth inhibition of OVCAR3-FSHR cell after treatment with DDP and FSH. Flow cytometry was used to analyze the change of cell cycle and percentage of apoptosis.
  
  
  RESULTS
  It was revealed that FSH decreased the growth inhibition induced by DDP. We also demonstrated that FSH reduced the S-phase percentage compared with the DDP only groups after treatment for 24 hours and reduced apoptosis percentage after 48 hours treatment with DDP.
  
  
  CONCLUSIONS
  It is suggested that FSH can protect the apoptosis induced by DDP. It also suggests that FSH may be an important chemoresistent reason for the chemotherapy of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Chun-fang
authors.initials: CF
authors.lastname: Huang
authors.forename: Dong-yuan
authors.initials: DY
authors.lastname: Liu
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Xu
authors.forename: Keng
authors.initials: K
authors.lastname: Shen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology; D011962:Receptors, FSH / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone; D002945:Cisplatin