Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation.
Jasmonic acid altered the accumulation of major anthocyanins in Vitis vinifera cell culture. Peonidin 3-glucoside content at day three was increased from 0.3 to 1.7 mg g(-1) dry cell wt while other major anthocyanins were increased by smaller increments. By day 14, the content of methylated and acylated anthocyanins (peonidin 3-p-coumaroylglucoside and malvidin 3-p-coumaroylglucoside) was 6.3 mg g(-1) DCW, in response to treatment with jasmonic acid, and comprising approximately 45% (w/w) of total anthocyanins. In comparison, the untreated control culture contained 1.2 mg g(-1) DCW which made up approximately 32% (w/w) of total anthocyanins. Light further enhanced anthocyanin accumulation induced by jasmonic acid elicitation. The content of peonidin 3-glucoside at day 3 was 6.6 mg g(-1) DCW, 22-fold higher than control cultures while the content in response to light irradiation alone was 0.6 mg g(-1) DCW. When a highly pigmented cell line was elicited with jasmonic acid total anthocyanins increased from 9.2 to 20.7 mg g(-1) DCW, but there was no change in the anthocyanin composition.
Curtin
Chris
C
Cooperative Research Centre for Bioproducts and Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, SA 5042, Adelaide, Australia.
Zhang
Wei
W
Franco
Chris
C
D000872:Anthocyanins / Q000096:biosynthesis* / Q000737:chemistry / Q000528:radiation effects; D018929:Cell Culture Techniques; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D005960:Glucosides / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects; D008027:Light; D054883:Oxylipins; D010860:Pigments, Biological / Q000528:radiation effects; D027843:Vitis / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
D000872:Anthocyanins; D003517:Cyclopentanes; D005960:Glucosides; D054883:Oxylipins; D010860:Pigments, Biological; C462279:cyanidin-3-O-beta-glucopyranoside; C480521:peonidin 3-glucoside; C011006:jasmonic acid; C458419:malvidin-3-glucoside