The activities of tissue xanthine oxidase and adenosine deaminase and the levels of hydroxyproline and nitric oxide in rat hearts subjected to doxorubicin: protective effect of erdosteine.
The aim of this experimental study was to investigate the effects of erdosteine, an antioxidant agent, on doxorubicin (DXR)-induced cardio-toxicity through nitric oxide (NO) levels, collagen synthesis, xanthine oxidase (XO) and adenosine deaminase (ADA) activities in rats. Rats were treated with erdosteine (10 mg/kg b.wt. per day, orally) or saline starting 2 days before administrating a single dose of DXR (20 mg/kg i.p.) or saline. At the 10th day of the DXR administration, hearts were removed under anesthesia for biochemical measurements. Enzyme activities as well as OH-proline and NO levels were found to be significantly increased in DXR group compared with the control group. All of the parameters studied except ADA activity were decreased significantly approximating to the control levels upon erdosteine administration. In conclusion, erdosteine seems to be an alternative agent for protection of cardiac tissue against DXR-induced cardio-toxicity through its regulatory effect on XO activity and NO level.
Fadillioglu
Ersin
E
Department of Physiology, Faculty of Medicine, Inonu University, TR 44069, Malatya, Turkey. efadillioglu@yahoo.com
Yilmaz
H Ramazan
HR
Erdogan
Hasan
H
Sogut
Sadik
S
D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000037:antagonists & inhibitors / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D004317:Doxorubicin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013864:Thioglycolates / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D013864:Thioglycolates; D013876:Thiophenes; D009569:Nitric Oxide; C048498:erdosteine; D004317:Doxorubicin; D014969:Xanthine Oxidase; D000243:Adenosine Deaminase; D006909:Hydroxyproline