Female-specific DNA sequences in geese.
1. The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
Huang
M C
MC
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan. mchuang@mail.nchu.edu.tw
Lin
W C
WC
Horng
Y M
YM
Rouvier
R
R
Huang
C W
CW
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D016172:DNA Fingerprinting / Q000662:veterinary; D017931:DNA Primers; D005260:Female; D005777:Geese / Q000235:genetics*; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*; D012737:Sex Factors
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA