Detection of rabbit corneal epithelium over amniotic membrane patch by white-light and laser confocal microscopy.
OBJECTIVE
To report confocal microscopic observation of rabbit corneal epithelium through amniotic membrane patch (AMP).


METHODS
Experimental study.


METHODS
Six rabbit eyes were patched by amniotic membrane. Then, white-light and laser confocal microscopic observation was performed.


RESULTS
Images of human amniotic epithelium, amniotic basement membrane, and amniotic stroma were obtained using both devices, followed by detection of rabbit corneal epithelium.


CONCLUSIONS
This study indicates that observation of corneal epithelium through AMP is possible in rabbit eyes using both these methods. This implies that both devices might be used clinically to observe the epithelial healing process under an AMP to better determine when it should be removed.
Kobayashi
Akira
A
Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa-prefecture, Japan. kobaya@kenroku.kanazawa-u.ac.jp
Yoshita
Tsuyoshi
T
Sugiyama
Kazuhisa
K
Tseng
Scheffer C G
SC
D000650:Amnion / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D019573:Epithelium, Corneal / Q000166:cytology* / Q000601:surgery; D007834:Lasers; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D011817:Rabbits