title: Cases of scrapie with unusual features in Norway and designation of a new type, Nor98.
abstract: Five cases of scrapie with unusual features have been diagnosed in Norway since 1998. The affected sheep showed neurological signs dominated by ataxia, and had the PrP genotypes homozygous A136 H154 Q171/ A136H154Q171 or heterozygous A136H154Q171/A136R154Q171, which are rarely associated with scrapie. Brain histopathology revealed neuropil vacuolisation essentially in the cerebellar and cerebral cortices; vacuolation was less prominent in the brainstem, and no lesions were observed at the level of the obex. The deposits of PrPSc were mainly in the cortex of the cerebellum and cerebrum, and no PrPSC was detectable by immunohistochemistry and ELISA in the lymphoid tissues investigated. Western blot analysis showed that the glycotype was different from other known scrapie strains and from the BSE strain. From a diagnostic point of view, these features indicate that this type of scrapie, designated Nor98, could have been overlooked and may be of significance for sampling in scrapie surveillance programmes.
authors.affiliation: National Veterinary Institute, Department of Pathology, Oslo, Norway.
authors.forename: S L
authors.initials: SL
authors.lastname: Benestad
authors.forename: P
authors.initials: P
authors.lastname: Sarradin
authors.forename: B
authors.initials: B
authors.lastname: Thu
authors.forename: J
authors.initials: J
authors.lastname: Schönheit
authors.forename: M A
authors.initials: MA
authors.lastname: Tranulis
authors.forename: B
authors.initials: B
authors.lastname: Bratberg
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D007150:Immunohistochemistry; D009664:Norway / Q000453:epidemiology; D018620:PrPSc Proteins / Q000235:genetics; D012608:Scrapie / Q000145:classification* / Q000453:epidemiology / Q000235:genetics / Q000473:pathology*; D034561:Sheep, Domestic / Q000235:genetics
chemical_list: D018620:PrPSc Proteins