title: Isolation and characterisation of pentraxin-like serum proteins from the common carp Cyprinus carpio.
abstract: There is increasing economic and ecological interest in the development of assays for the early detection of infection, disease activity and environmental stress in marine and freshwater animals. In humans the serum pentraxin C-reactive protein (CRP) is universally used as a clinical indicator of inflammation and underlying infection. As a first step towards assessing the potential of an immunoassay for CRP in fish, we have isolated and characterised common carp Cyprinus carpio CRP and a highly specific and sensitive anti-carp CRP polyclonal antibody has been raised. The results show levels of CRP in healthy fish similar to those found in healthy humans. A protein of unknown function, which displays the characteristic calcium-dependent phosphate monoester binding exhibited by CRP and some similarity to the known fish pentraxin sequences, has also been identified.
authors.affiliation: School of Life Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK.
authors.forename: Jamie R
authors.initials: JR
authors.lastname: Cartwright
authors.forename: Hazel A
authors.initials: HA
authors.lastname: Tharia
authors.forename: Ian
authors.initials: I
authors.lastname: Burns
authors.forename: Annette K
authors.initials: AK
authors.lastname: Shrive
authors.forename: David
authors.initials: D
authors.lastname: Hoole
authors.forename: Trevor J
authors.initials: TJ
authors.lastname: Greenhough
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002097:C-Reactive Protein / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002347:Carps / Q000235:genetics / Q000276:immunology*; D002846:Chromatography, Affinity; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D000906:Antibodies; D002097:C-Reactive Protein