Absolute gene expression patterns of thioredoxin and glutaredoxin redox systems in mouse.
This work provides the first absolute expression patterns of genes coding for all known components of both thioredoxin (Trx) and glutaredoxin (Grx) systems in mouse: Trx1, Trx2, Grx1, Grx2, TrxR1, TrxR2, thioredoxin/glutathione reductase, and glutathione reductase. We devised a novel assay that, combining the advantages of multiplex and real-time PCR, streamlines the quantitation of the actual mRNA copy numbers in whole-animal experiments. Quantitations reported establish differences among adult organs and embryonic stages, compare mRNA decay rates, explore the significance of alternative mRNA isoforms derived from TrxR1 and Grx2 genes, and examine the time-course expression upon superoxide stress promoted by paraquat. Collectively, these quantitations show: i) unique expression profiles for each transcript and mouse organ examined, yet with some general trends like the higher amounts of mRNA species coding for thioredoxins than those coding for the reductases that control their redox states and activities; ii) continuous expression during embryogenesis with outstanding up-regulations of Trx1 and TrxR1 mRNAs in specific temporal sequences; iii) drastic differences in mRNA stability, liver decay rates range from 2.8 h (thioredoxin/glutathione reductase) to >/= 35 h (Trx1 and Trx2), and directly correlate with mRNA steady-state values; iv) testis-specific differences in the amounts (relative to total isoforms) of transcripts yielding the mitochondrial Grx2a and 67-kDa TrxR1 variants; and v) coordinated up-regulation of TrxR1 and glutathione reductase mRNAs in response to superoxide stress in an organ-specific manner. Further insights into in vivo roles of these redox systems should be gained from more focused studies of the mechanisms underlying the vast differences reported here at the transcript level.
Jurado
Juan
J
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071 Córdoba, Spain.
Prieto-Alamo
María-José
MJ
Madrid-Rísquez
José
J
Pueyo
Carmen
C
D000818:Animals; D005786:Gene Expression Regulation; D054477:Glutaredoxins; D005980:Glutathione Reductase / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010088:Oxidoreductases; D010269:Paraquat / Q000494:pharmacology; D014176:Protein Biosynthesis; D020033:Protein Isoforms; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054481:Thioredoxin Reductase 1; D054482:Thioredoxin Reductase 2; D013880:Thioredoxin-Disulfide Reductase / Q000096:biosynthesis; D013879:Thioredoxins / Q000096:biosynthesis*; D013997:Time Factors
C516006:Glrx protein, mouse; C516009:Glrx2 protein, mouse; D054477:Glutaredoxins; D008565:Membrane Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C516112:Txn2 protein, mouse; C491200:Txndc2 protein, mouse; D013879:Thioredoxins; D012313:RNA; D010088:Oxidoreductases; D005980:Glutathione Reductase; D054481:Thioredoxin Reductase 1; D054482:Thioredoxin Reductase 2; D013880:Thioredoxin-Disulfide Reductase; C516125:Txnrd1 protein, mouse; C516128:Txnrd2 protein, mouse; D010269:Paraquat