Adenosine A2A receptors regulate oxidative stress formation in rat pheochromocytoma PC12 cells during serum deprivation.
Activation of A2A adenosine receptors (A2A-Rs) was found to prevent reactive oxygen species (ROS) formation and apoptosis in serum-deprived pheochromocytoma PC12 cells. A protein kinase A (PKA) inhibitor not only blocked the anti-apoptotic roles of A2A-R but also reversed A2A-R-induced suppression of ROS formation, indicating a PKA-dependent pathway in preventing ROS formation and apoptosis. PKA stimulators and antioxidants prevented serum-deprived ROS formation and apoptosis. In addition, A2A-R activation also prevented H2O2-induced cell death, further suggesting the protective roles of A2A-R in antagonizing oxidative stress and cell death. Finally, antioxidative system-interrupting agents attenuated A2A-R-mediated protection. Taken together, these data indicate that PKA-dependent regulation and attenuation of oxidative stress by A2A-R may play at least some roles in preventing serum-deprived PC12 cell apoptosis.
Huang
Nai-Kuei
NK
National Research Institute of Chinese Medicine, No. 155-1, Li-Nung Street, Sec. 2, Ship-Pai, Peitou, Taipei 112, Taiwan, ROC. andrew@mail.nricm.edu.tw
D000818:Animals; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D016716:PC12 Cells; D058906:Purinergic P1 Receptor Agonists; D051381:Rats; D043705:Receptor, Adenosine A2A; D018047:Receptors, Purinergic P1 / Q000502:physiology*
D016895:Culture Media, Serum-Free; D058906:Purinergic P1 Receptor Agonists; D043705:Receptor, Adenosine A2A; D018047:Receptors, Purinergic P1; D017868:Cyclic AMP-Dependent Protein Kinases