[Molecular responses of rice (Oryza sativa L.) to the stress of lowly enhanced UV-B radiation].
The approach of mRNA differential display (DD) was used to analyze the gene differential expression of rice seedling subjected to lowly enhanced Ultraviolet-B radiation. The results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as RUVB1 and RUVB2 respectively, which could be recovered and re-amplified, were specifically induced. Sequence analysis for RUVB2 through Internet BLAST searching indicated that it was more than 95% identities with three rice ESTs, but very low similarity to others in the GenBank, and was mapped on the OSJNBb0091N21BAC(GenBank accession No.: AC091122) in Oryza sativa L.
Zeng
Jianmin
J
Agroecological Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Lin
Wenxiong
W
Liang
Kangjing
K
Chen
Fangyu
F
Guo
Yuchun
Y
Tang
Lina
L
He
Huaqin
H
Wang
Jingyuan
J
Liang
Yiyuan
Y
D012275:Oryza / Q000235:genetics / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays