Identification and characterization of NIF3L1 BP1, a novel cytoplasmic interaction partner of the NIF3L1 protein.
The NIF3L1 protein is strongly conserved during evolution from bacteria to mammals and recently its function in neuronal differentiation has been demonstrated. In the present study we identified novel binding partners of human NIF3L1 by screening a HeLa cDNA-library using the yeast two-hybrid system. We could show that the NIF3L1 protein is interacting with itself and with the NIF3L1 binding protein 1 (NIF3L1 BP1), a novel protein of 23.67kDa bearing a putative leucine zipper domain. Furthermore, both interactions were confirmed using the mammalian two-hybrid system. Deletion analyses clearly demonstrated that a C-terminal region of 100 amino acids of the NIF3L1 BP1 is sufficient for the interaction with NIF3L1. The NIF3L1 BP1 is ubiquitously expressed and cotransfection experiments revealed that NIF3L1 and NIF3L1 BP1 interact in the cytoplasm of human LNCaP cells. This study provides novel insights into the cellular function of the NIF3L1 protein.
Tascou
S
S
Institute of Human Genetics, Georg-August University Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany.
Kang
T W
TW
Trappe
R
R
Engel
W
W
Burfeind
P
P
D016475:3T3 Cells / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D056970:Co-Repressor Proteins; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D019154:Protein Splicing; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques
D056970:Co-Repressor Proteins; C419199:NIF3L1 protein, human; C486006:Nif3l1 protein, mouse; D011506:Proteins; D014157:Transcription Factors