Liquid infrared atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry of sialylated carbohydrates.
A 2.94-microm Er:YAG laser for IR atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used for the analysis of sialylated oligosaccharides. This approach provided the opportunity to utilize liquid matrixes and is effective in determining structural features--sequence, branching, and linkage--of intact, fully sialylated molecular species.
Von Seggern
Christopher E
CE
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA.
Moyer
Susanne C
SC
Cotter
Robert J
RJ
D001274:Atmospheric Pressure; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D007259:Infrared Rays; D007834:Lasers; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002241:Carbohydrates; D012794:Sialic Acids