title: Telomerase activity and cell apoptosis in colon cancer cell by human telomerase reverse transcriptase gene antisense oligodeoxynucleotide.
abstract: OBJECTIVE
  To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (As-ODN) on telomerase activity and cell apoptosis in colon cancer cell line SW480.
  
  
  METHODS
  As-ODN was transfected into cells SW480 by liposomal transfection. Cultured cells were divided into three groups: ASODN (5'GGAGCGCGCGGCATCGCGGG-3'), sense oligodeoxynucleotide (5'-CCCGCGATGCCGCGCGCTCC-3', S-ODN) and control. The concentration of oligodeoxynucleotide and liposome was 10 micromol/L and 16 mg/L, respectively. The activity of telomerase was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA), and cell apoptosis was observed by morphology and flow cytometry in each group.
  
  
  RESULTS
  Telomerase activity began to be down-regulated or inhibited when cells SW480 were treated with As-ODN for 72 h, and cell apoptosis was induced.
  
  
  CONCLUSIONS
  It is suggested that hTERT As-ODN might specially inhibit the activity of telomerase in colon cancer cells and it is further proved that the hTERT gene has a significant correlation with telomerase activity. Further evidence is needed to prove whether hTERT As-ODN is a potential tool for the treatment of colon cancer.
authors.affiliation: Department of Gastroenterology, Renming Hospital of Wuhan University, Hubei Province China. weijin@hs.hb.cninfo.net
authors.forename: Ying-An
authors.initials: YA
authors.lastname: Jiang
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Luo
authors.forename: You-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Fan
authors.forename: Chong-Qing
authors.initials: CQ
authors.lastname: Jiang
authors.forename: Wei-Jin
authors.initials: WJ
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D003110:Colonic Neoplasms / Q000201:enzymology / Q000503:physiopathology*; D004268:DNA-Binding Proteins; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D019098:Telomerase