Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy.
The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorbance of the amide II band, and increased absorbance at around 1600 cm(-1). These results suggest that exposure to the ELF magnetic field has reversible effects on the N-H inplane bending and C-N stretching vibrations of peptide linkages, and changes the secondary structures of alpha-helix and beta-sheet in cell membrane proteins.
Ikehara
Toshitaka
T
Department of Physiology, Course of Preventive Medicine, School of Medicine, The University of Tokushima, Tokushima, Japan.
Yamaguchi
Hisao
H
Hosokawa
Keiko
K
Miyamoto
Hiroshi
H
Aizawa
Katsuo
K
D000418:Albumins / Q000737:chemistry / Q000528:radiation effects; D000577:Amides / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D005916:Globulins / Q000737:chemistry / Q000528:radiation effects; D006367:HeLa Cells / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000737:chemistry* / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects*; D011846:Radio Waves; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000418:Albumins; D000577:Amides; D005916:Globulins; D008565:Membrane Proteins