Measurement of molt-inhibiting hormone titer in hemolymph of the American crayfish, Procambarus clarkii, by time-resolved fluoroimmunoassay.
In order to determine the titer of molt-inhibiting hormone (Prc-MIH) in the hemolymph of the American crayfish Procambarus clarkii, a time-resolved fluoroimmunoassay (TR-FIA) was established using specific antibodies against N-terminal and C-terminal segments of Prc-MIH. The lowest limit of detection of Prc-MIH in TR-FIA was 10 amol/assay. The Prc-MIH titers in the hemolymph were 6.53 fmol/ml at the intermolt stage and 1.25 fmol/ml at the early premolt stage. This result is consistent with the long-known hypothesis that the Y-organ is inhibited by MIH during the intermolt stage, whereas the Y-organ is activated by being freed from the inhibitory regulation of MIH.
Nakatsuji
Teruaki
T
Department of Life and Functional Material Science, Graduate School of Natural Sciences, Konan University, Kobe, Japan. dn122001@center.konan-u.ac.jp
Sonobe
Haruyuki
H
D000818:Animals; D003400:Astacoidea / Q000276:immunology*; D015200:Fluoroimmunoassay; D006458:Hemolymph / Q000737:chemistry; D008297:Male; D018978:Molting / Q000502:physiology*; D009479:Neuropeptides / Q000097:blood*
D009479:Neuropeptides; C101529:molt-inhibiting hormone, crayfish