Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing.
A set of seven Sm proteins assemble on the Sm-binding site of spliceosomal U snRNAs to form the ring-shaped Sm core. The U7 snRNP involved in histone RNA 3' processing contains a structurally similar but biochemically unique Sm core in which two of these proteins, Sm D1 and D2, are replaced by Lsm10 and by another as yet unknown component. Here we characterize this factor, termed Lsm11, as a novel Sm-like protein with apparently two distinct functions. In vitro studies suggest that its long N-terminal part mediates an important step in histone mRNA 3'-end cleavage, most likely by recruiting a zinc finger protein previously identified as a processing factor. In contrast, the C-terminal part, which comprises two Sm motifs interrupted by an unusually long spacer, is sufficient to assemble with U7, but not U1, snRNA. Assembly of this U7-specific Sm core depends on the noncanonical Sm-binding site of U7 snRNA. Moreover, it is facilitated by a specialized SMN complex that contains Lsm10 and Lsm11 but lacks Sm D1/D2. Thus, the U7-specific Lsm11 protein not only specifies the assembly of the U7 Sm core but also fulfills an important role in U7 snRNP-mediated histone mRNA processing.
Pillai
Ramesh S
RS
Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland.
Grimmler
Matthias
M
Meister
Gunter
G
Will
Cindy L
CL
Lührmann
Reinhard
R
Fischer
Utz
U
Schümperli
Daniel
D
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins; D020885:Ribonucleoprotein, U7 Small Nuclear / Q000096:biosynthesis / Q000737:chemistry*; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D055517:snRNP Core Proteins
D001324:Autoantigens; D006657:Histones; C477938:Lsm11 protein, mouse; D016601:RNA-Binding Proteins; D020885:Ribonucleoprotein, U7 Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; C526417:SNRPN protein, human; D055517:snRNP Core Proteins