title: Dissecting the role of lymphotoxin in lymphoid organs by conditional targeting.
abstract: Mice with inactivation of lymphotoxin beta receptor (LTbetaR) system have profound defects in the development and maintenance of peripheral lymphoid organs. As surface LT is expressed by lymphocytes, natural killer cells, and lymphoid tissue-initiating cells as well as by some other cell types, we dissected cell type-specific LT contribution into the complex LT-deficient phenotype by conditional gene targeting. B-LTbeta knockout (KO) mice displayed an intermediate phenotype in spleen as compared with mice with complete LTbeta deficiency. In contrast, T-LTbeta KO mice displayed normal structure of the spleen. However, inactivation of LTbeta in both T and B cells resulted in additional defects in the structure of the marginal zone and in the development of follicular dendritic cells in spleen. Structure of lymph nodes (LN) and Peyer's patches (PP) was normal in both B-LTbeta KO and T- and B-LTbeta KO mice, except that PPs were of reduced size. When compared across the panel of lymphocyte-specific LT KOs, the defects in antibody responses to T-cell-dependent antigens correlated with the severity of defects in spleen structure. Expression of CCL21 and CCL19 chemokines was not affected in spleen, LN and PP of B-LTbeta KO and T- and B-LTbeta KO mice, while CXCL13 was slightly reduced only in spleen. Collectively, our data suggest the following: (i). requirements for LT signaling to support architecture of spleen, LN and PP are different; (ii). LT complex expressed by B cells plays a major role in the maintenance of spleen structure, while surface LT expressed by T cells provides a complementary but distinct signal; and (iii). in a non-transgenic model, expression of lymphoid tissue chemokines is only minimally dependent on the expression of surface LT complex on B and T lymphocytes.
authors.affiliation: Laboratory of Molecular Immunology,Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. tumanov97@yahoo.com
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Tumanov
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Grivennikov
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shakhov
authors.forename: Stanislav A
authors.initials: SA
authors.lastname: Rybtsov
authors.forename: Ekaterina P
authors.initials: EP
authors.lastname: Koroleva
authors.forename: Junji
authors.initials: J
authors.lastname: Takeda
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Nedospasov
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Kuprash
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015398:Signal Transduction; D013154:Spleen / Q000002:abnormalities / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D018925:Chemokines; D008233:Lymphotoxin-alpha