Hyperthermia triggers apoptosis and affects cell adhesiveness in human neuroblastoma cells.
Hyperthermia is a known apoptotic inducer and has been recently utilized in combination with chemo-and/or radiotherapy in cancer treatment. In this study we have described its effect on SK-N-MC human neuroblastoma tumor cells, a line which grows as a double adherent and floating population. Considering this particular culture behavior, we also investigated the relationship between hyperthermia and cell adhesiveness by evaluating integrin expression, namely CD11a, which is, as known, closely correlated to cell adhesion properties. By a multiple, ultrastructural and flow cytometrical approach, we have demonstrated that hyperthermia, while triggering apoptosis, also determines a CD11a surface expression decrease in apoptotic and living cells. We thus suggest a further role for this treatment, which, affecting adhesion mechanisms, could down-regulate metastatic diffusion.
Luchetti
F
F
Istituto di Scienze Morfologiche, Urbino University, Italy.
Canonico
B
B
Della Felice
M
M
Burattini
S
S
Battistelli
M
M
Papa
S
S
Falcieri
E
E
D017209:Apoptosis / Q000502:physiology*; D040881:CD11a Antigen / Q000096:biosynthesis; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D004396:Coloring Agents; D005334:Fever / Q000473:pathology*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009336:Necrosis; D009447:Neuroblastoma / Q000473:pathology* / Q000648:ultrastructure; D009993:Osmium Tetroxide; D016707:Tissue Fixation
D040881:CD11a Antigen; D004396:Coloring Agents; D005456:Fluorescent Dyes; D016169:Lymphocyte Function-Associated Antigen-1; D016650:Fluorescein-5-isothiocyanate; D009993:Osmium Tetroxide