title: Absence of inhibition of cutaneous wound healing in mice by oral doxycycline.
abstract: Temporally controlled expression of genes in transgenic mice has advanced our understanding of many physiological processes. One of the more common modes of acutely altering gene levels involves the doxycycline-regulated "tet-on/tet-off" systems. There has been concern that the administration of doxycycline in itself might compromise many aspects of wound repair. Here we report that oral ad libitum administration of doxycycline (2 mg/ml in drinking water) to mice does not alter dermal or epidermal wound healing. The healing of both full- and partial-thickness skin wounds proceeded similarly regardless of doxycycline administration; in fact, collagen organization and tensile strength development appeared to accelerate in the presence of doxycycline. These data suggest that wound healing studies incorporating transgene expression can utilize tet-on/tet-off regulation of gene expression without interference from doxycycline.
authors.affiliation: Department of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA. hebda@pitt.edu
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Hebda
authors.forename: Diana
authors.initials: D
authors.lastname: Whaley
authors.forename: Hyung-Gyoon
authors.initials: HG
authors.lastname: Kim
authors.forename: Alan
authors.initials: A
authors.lastname: Wells
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D004318:Doxycycline / Q000008:administration & dosage*; D005109:Extracellular Matrix / Q000187:drug effects; D015603:Keratinocytes / Q000187:drug effects*; D051379:Mice; D008822:Mice, Transgenic; D012867:Skin / Q000187:drug effects / Q000293:injuries; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline