The 'potyvirid primers' will probably provide phylogenetically informative DNA fragments from all species of Potyviridae.
A survey of gene sequences of species of Potyviridae, the most numerous family of plant viruses, has shown that the -GNNS- encoding region of the NIb gene is present in all c. 300 publicly available sequences of that region. We also report that relationships inferred from the sequence of the 1.6-2.1 kb portion of the genome between the -GNNS- encoding region and its 3' terminus reflect those of the remainder of the genome. Thus, the use for RT-PCR tests of the 'potyvirid primers' based on the -GNNS- encoding region and the 3' terminal poly-A region of the genome, will yield DNA fragments, the sequences of which are very likely to correctly identify viruses from which they were obtained.
Gibbs
Adrian J
AJ
School of Botany and Zoology, Faculty of Science, Australian National University, Canberra, ACT 0200, Australia. adrian.gibbs@anu.edu.au
Mackenzie
Anne M
AM
Gibbs
Mark J
MJ
D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004279:DNA, Viral