Reactive oxygen species and ethylene play a positive role in lateral root base nodulation of a semiaquatic legume.
Lateral root base nodulation on the tropical, semiaquatic legume Sesbania rostrata results from two coordinated, Nod factor-dependent processes: formation of intercellular infection pockets and induction of cell division. Infection pocket formation is associated with cell death and production of hydrogen peroxide. Pharmacological experiments showed that ethylene and reactive oxygen species mediate Nod factor responses and are required for nodule initiation, whereby induction of division and infection could not be uncoupled. Application of purified Nod factors triggered cell division, and both Nod factors and ethylene induced cavities and cell death features in the root cortex. Thus, in S. rostrata, ethylene and reactive oxygen species act downstream from the Nod factors in pathways that lead to formation of infection pockets and initiation of nodule primordia.
D'Haeze
Wim
W
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
De Rycke
Riet
R
Mathis
René
R
Goormachtig
Sofie
S
Pagnotta
Sophie
S
Verplancke
Christa
C
Capoen
Ward
W
Holsters
Marcelle
M
D005030:Ethylenes / Q000378:metabolism*; D007887:Fabaceae / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism*
D005030:Ethylenes; D017382:Reactive Oxygen Species; C036216:ethylene