title: Ichthyotoxic and anticarcinogenic effects of triterpenoids from Sandoricum koetjape bark.
abstract: After bioassay-guided fractionation of the extract from Sandoricum koetjape bark, which exhibited significant toxicity to killifish (Oryzias latipes), two ichthyotoxic triterpenoids were isolated and characterized as koetjapic acid and 3-oxo-olean-12-en-29-oic acid. These constituents, along with non-toxic katonic acid, had a remarkable inhibitory effect on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA), which is a preliminary in vitro screening method for possible anti-tumor-promoting agents. Of the triterpenoids active in vitro, koetjapic acid appears to be a promising cancer chemopreventive agent, since it significantly delayed tumor promotion in two-stage mouse skin carcinogenesis induced by 7,12-dimethylbenz(a)anthracene and promoted by TPA.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Intan Safinar
authors.initials: IS
authors.lastname: Ismail
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Teruo
authors.initials: T
authors.lastname: Mukainaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Higashihara
authors.forename: Fumio
authors.initials: F
authors.lastname: Enjo
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D019309:Epstein-Barr Virus Nuclear Antigens / Q000187:drug effects; D005260:Female; D023781:Fundulidae / Q000502:physiology*; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity*
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D019309:Epstein-Barr Virus Nuclear Antigens; D010936:Plant Extracts; D014315:Triterpenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate