Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.
A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.
Gray
M
M
University of South Florida, College of Marine Science, St. Petersburg, Florida 33701, USA.
Wawrik
B
B
Paul
J
J
Casper
E
E
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004141:Dinoflagellida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000469:parasitology
D017931:DNA Primers