Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs.
N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated.
Akimoto-Tomiyama
Chiharu
C
Department of Biochemistry, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba 305-8602, Japan. akimotoc@nias.affrc.go.jp
Sakata
Katsumi
K
Yazaki
Junshi
J
Nakamura
Keiko
K
Fujii
Fumiko
F
Shimbo
Kanako
K
Yamamoto
Kimiko
K
Sasaki
Takuji
T
Kishimoto
Naoki
N
Kikuchi
Shoshi
S
Shibuya
Naoto
N
Minami
Eiichi
E
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009844:Oligosaccharides / Q000494:pharmacology*; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D009844:Oligosaccharides; D011494:Protein Kinases; D018749:RNA, Plant; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C082723:N-acetylchitooctaose