title: Morphometric classification of hairy cell leukemia in bone marrow trephine biopsy.
abstract: OBJECTIVE
  To analyze cytologic and histologic parameters in bone marrow trephine biopsy in an attempt to define heterogeneity of hairy cell leukemia cells.
  
  
  METHODS
  The study group consisted of 28 trephine biopsies. Immunohistochemistry for CD20 antigen was used. Image processing and measurements were performed with AnalySIS 3.0 image analysis system (Soft Imaging System GmbH, Germany) and custom built programs. For planimetric measurements of nuclei, automatic segmentation was implemented. The measured parameters were: surface area, perimeter, minimum, mean and maximum diameter, and a set of form factors. Relative volumes of bone trabeculae, adipose tissue, hematopoietic tissue and neoplastic infiltrate were assessed by the point counting method. Nuclear volume was measured by the point sampled intercept method. Bone marrow fibrosis was assessed using a curvilinear line test system.
  
  
  RESULTS
  Significant variability of cell nuclei was found, and their classification into 3 types was possible. The relative frequency of those types was different in various cases and allowed subdivision of cases into 3 groups that differed in some clinical and histologic manifestations.
  
  
  CONCLUSIONS
  The present study demonstrated the heterogeneity of cell populations of hairy cell leukemia.
authors.affiliation: Department of Pathomorphology, Collegium Medicum, Jagiellonian University, ul. Grzegorzecka 16, 31-531 Krakow, Poland. mpokon@cyf-kr.edu.pl
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Okon
authors.forename: Anna
authors.initials: A
authors.lastname: Szumera
authors.forename: Boleslaw
authors.initials: B
authors.lastname: Papla
authors.forename: Iwona
authors.initials: I
authors.lastname: Pietkun
authors.forename: Andrzej
authors.initials: A
authors.lastname: Zdunczyk
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Rucinska
authors.forename: Aleksander B
authors.initials: AB
authors.lastname: Skotnicki
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stachura
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D001773:Blood Cells / Q000473:pathology; D001854:Bone Marrow Cells / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure; D048429:Cell Size; D005260:Female; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000145:classification* / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged