Overexpression of the dual-specificity MAPK phosphatase PYST2 in acute leukemia.
In a previous study we used gene expression arrays to identify genes that are more highly expressed by leukemic than by non-leukemic leukocytes from acute myelogenous leukemia patients. One of such genes was Phosphorylates tyrosine serine threonine 2 (PYST2), a dual-specificity Mitogen-activated protein (MAP) kinase (MAPK) phosphatase. In the present study, high levels of PYST2 mRNA were detected by RT-PCR and by Northern blotting in bone marrow (BM) leukocytes and in peripheral blood mononuclear cells from additional eight AML patients. No PYST2 mRNA was detected in nine out of twelve samples of Peripheral blood mononuclear cells (PBMC) from healthy blood bank donors and very low levels were detected by the same techniques in the other three PBMC samples from the healthy donors. Relatively high levels of PYST2 were detected in a variety of myeloid leukemia and other cancer cell lines. In view of the potential role played by PYST2 in MAPK signaling cascades we propose that an over expression of PYST2 in malignant cells may reflect a disrupted or an altered MAPK signaling pathway in malignancy processes.
Levy-Nissenbaum
Orlev
O
Department of Cell Research and Immunology, George S Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.
Sagi-Assif
Orit
O
Raanani
Pia
P
Avigdor
Abraham
A
Ben-Bassat
Isaac
I
Witz
Isaac P
IP
D000208:Acute Disease; D000328:Adult; D015152:Blotting, Northern; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D017931:DNA Primers; D054638:Dual Specificity Phosphatase 1; D054637:Dual-Specificity Phosphatases; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010749:Phosphoprotein Phosphatases; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology*
D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; C517019:DUSP1 protein, human; C107523:DUSP7 protein, human; D054638:Dual Specificity Phosphatase 1; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases