EphB-ephrinB bi-directional endocytosis terminates adhesion allowing contact mediated repulsion.
Eph receptors and their membrane-associated ephrin ligands mediate cell-cell repulsion to guide migrating cells and axons. Repulsion requires that the ligand-receptor complex be removed from the cell surface, for example by proteolytic processing of the ephrin ectodomain. Here we show that cell contact-induced EphB-ephrinB complexes are rapidly endocytosed during the retraction of cells and neuronal growth cones. Endocytosis occurs in a bi-directional manner that comprises of full-length receptor and ligand complexes. Endocytosis is sufficient to promote cell detachment and seems necessary for axon withdrawal during growth cone collapse. Here, we show a mechanism for the termination of adhesion and the promotion of cell repulsion after intercellular (trans) interaction between two transmembrane proteins.
Zimmer
Manuel
M
Max-Planck Institute of Neurobiology, Am Klopferspitz 18A, D-82152 Martinsried, Germany.
Palmer
Amparo
A
Köhler
Jenny
J
Klein
Rüdiger
R
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004705:Endocytosis / Q000502:physiology*; D036387:Ephrin-B1 / Q000235:genetics / Q000378:metabolism*; D036388:Ephrin-B2 / Q000235:genetics / Q000378:metabolism*; D020439:Growth Cones / Q000378:metabolism; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D036081:Receptors, Eph Family / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D036387:Ephrin-B1; D036388:Ephrin-B2; D011993:Recombinant Fusion Proteins; D036081:Receptors, Eph Family