Role of the charge in continuous beds in the chiral separation of hydroxy acids by ligand-exchange capillary electrochromatography.
This paper deals with the chiral separation of hydroxy acids using diallyl-dimethylammonium chloride as a positive charge-providing agent in the continuous bed. The chiral continuous bed was prepared by in situ copolymerization of monomers, including an L-4-hydroxyproline derivative as a chiral selector. This phase was applied to the chiral separation of hydroxy monocarboxylic acids and hydroxy dicarboxylic acids, respectively. The influence of both the selector concentration and the charge-providing agent on retention and separation was investigated.
Lecnik
Oliver
O
Institute of Pharmaceutical Chemistry and PharmaceuticalTechnology, Karl-Franzens University, Graz, Austria.
Gübitz
Gerald
G
Schmid
Martin G
MG
D002852:Chromatography, Ion Exchange / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006880:Hydroxy Acids / Q000032:analysis / Q000737:chemistry*; D006909:Hydroxyproline / Q000737:chemistry; D007773:Lactates / Q000032:analysis / Q000737:chemistry; D008024:Ligands; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013237:Stereoisomerism; D013997:Time Factors
D006880:Hydroxy Acids; D007773:Lactates; D008024:Ligands; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C017648:3-phenyllactic acid; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D006909:Hydroxyproline