Molecular cloning and characterization of a novel mannose-binding lectin gene from Amorphophallus konjac.
A new lectin gene was cloned from Amorphophallus konjac. The full-length cDNA of Amorphophallus konjac agglutinin (aka) was 736 bp and contained a 474 bp open reading frame encoding a 158 amino acid protein. Homology analysis revealed that the lectin from this Araceae species belonged to the superfamily of monocot mannose-binding proteins. Molecular modeling of AKA indicated that the three-dimensional structure of AKA strongly resembles that of the snowdrop lectin. Southern blot analysis of the genomic DNA revealed that aka belonged to a low-copy gene family. Northern blot analysis demonstrated that aka expression was tissue-specific with the strongest expression being found in root.
Fei
Jiong
J
Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Plant Biotechnology Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200030, China.
Liao
Zhihua
Z
Chai
Yourong
Y
Pang
Yongzhen
Y
Yao
Jianhong
J
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D031056:Amorphophallus / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D037601:Mannose-Binding Lectin / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D018076:DNA, Complementary; D037601:Mannose-Binding Lectin; D012333:RNA, Messenger