A comprehensive approach to the analysis of matrix-assisted laser desorption/ionization-time of flight proteomics spectra from serum samples.
For our analysis of the data from the First Annual Proteomics Data Mining Conference, we attempted to discriminate between 24 disease spectra (group A) and 17 normal spectra (group B). First, we processed the raw spectra by (i) correcting for additive sinusoidal noise (periodic on the time scale) affecting most spectra, (ii) correcting for the overall baseline level, (iii) normalizing, (iv) recombining fractions, and (v) using variable-width windows for data reduction. Also, we identified a set of polymeric peaks (at multiples of 180.6 Da) that is present in several normal spectra (B1-B8). After data processing, we found the intensities at the following mass to charge (m/z) values to be useful discriminators: 3077, 12 886 and 74 263. Using these values, we were able to achieve an overall classification accuracy of 38/41 (92.6%). Perfect classification could be achieved by adding two additional peaks, at 2476 and 6955. We identified these values by applying a genetic algorithm to a filtered list of m/z values using Mahalanobis distance between the group means as a fitness function.
Baggerly
Keith A
KA
Department of Biostatistics, UT M.D. Anderson Cancer Center, Houston, TX 77030, USA. kabagg@mdanderson.org
Morris
Jeffrey S
JS
Wang
Jing
J
Gold
David
D
Xiao
Lian-Chun
LC
Coombes
Kevin R
KR
D001798:Blood Proteins / Q000032:analysis*; D016002:Discriminant Analysis; D001330:Electronic Data Processing; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001798:Blood Proteins