title: Multiplex PCRs for identification of necrotoxigenic Escherichia coli.
abstract: Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.
authors.affiliation: University of Liège, Faculty of Veterinary Medicine, Laboratory of Bacteriology, Sart-Tilman B43a, B-4000 Liège, Belgium. svanbost@ulg.ac.be
authors.forename: Sigrid
authors.initials: S
authors.lastname: Van Bost
authors.forename: Etienne
authors.initials: E
authors.lastname: Jacquemin
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
authors.forename: Jacques
authors.initials: J
authors.lastname: Mainil
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D002417:Cattle; D003603:Cytotoxins / Q000235:genetics; D004285:Dogs; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D006801:Humans; D006877:Hydroxamic Acids; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine; D037521:Virulence Factors
chemical_list: D018830:Adhesins, Escherichia coli; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006877:Hydroxamic Acids; D037521:Virulence Factors; C067044:cytolethal distending toxin; C060296:cytotoxic necrotizing factor type 1; C031819:aerobactin