Diamond paste based immunosensor for the determination of azidothymidine.
An amperometric immunosensor, based on diamond paste (diamond powder and paraffin oil), has been constructed for the assay of azidothymidine (AZT). The diamond paste is impregnated with anti-AZT. The immunosensor can be used reliably for the assay of azidothymidine in its pharmaceutical formulations. The potential used for azidothymidine assay was + 240 mV vs. Ag/AgCl electrode. The surface of the immunosensor can be regenerated by simply polishing, thereby obtaining fresh immunocomposite ready to be used in a new assay. The new amperometric immunosensor, based on diamond paste, gives reliable results for the assay of AZT as raw material and from its pharmaceutical formulation.
Stefan
Raluca-Ioana
RI
Department of Chemistry, University of Pretoria, Pretoria, South Africa. Raluca.stefan@chem.up.ac.za
Bokretsion
Rahel Girmai
RG
D018130:Diamond; D004563:Electrochemistry; D004566:Electrodes; D007118:Immunoassay / Q000379:methods*; D015215:Zidovudine / Q000032:analysis* / Q000276:immunology
D015215:Zidovudine; D018130:Diamond