[Observation on tissue factor pathway during the onset of acute cerebral infarction].
OBJECTIVE
To establish the possible relationship between some coagulation factors and the onset of acute cerebral infarction (ACI).


METHODS
The study population consisted of 71 patients with ACI confirmed by CT and 50 age-matched healthy volunteers. Blood samples were obtained during the onset period of ACI. Tissue factor (TF) and tissue factor pathway inhibitor (TFPI) activity in plasma were assayed with the chromogenic assay. Plasma TF and TFPI antigen were measured with enzyme linked immunoadsorbent assay (ELISA). Plasma F VII coagulation activity (F VII: C) and F VIII coagulation activity (F VIII: C) were developed in the one-stage system. Plasma prothrombin (FII) was determined with Ecarin assay. Plasma fibrinogen (Fbg) was measured with thrombin assay. Plasma antithrombin III activity (ATIII) was determined using heparin cofactor activity assay.


RESULTS
Compared with the control, plasma TF activity and antigen in patients with ACI were significantly higher (both P<0.05). But plasma TFPI activity and antigen were remarkably lower in the ACI group (both P<0.05). Plasma F VII: C was significantly higher (P<0.01), and F VIII: C was markedly lower (P<0.05). Plasma FII was remarkably higher (P<0.01). Similarly the Fbg was significantly higher in the ACI than that in the control group (P<0.01), whereas ATIII was significantly lower (P<0.01).


CONCLUSIONS
The initiation of TF pathway is contributed to the onset of ACI and the blood is in hypercoagulable state during the early period of ACI.
Wen
Zhi-bin
ZB
Thrombosis and Hemostasis Lab, Department of Physiology, Xiangya Medical University, Central-South University, Changsha 410078, Hunan, China. wenzhibin2002@yahoo.com.cn
Xiong
Shi-long
SL
He
Xiao-fan
XF
He
Mei-xia
MX
He
Shi-lin
SL
Xie
Qin-zhi
QZ
Jian
Zai-fu
ZF
Chen
Fang-ping
FP
Xiao
Bo
B
Yang
Qi-dong
QD
D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D001779:Blood Coagulation Factors / Q000032:analysis; D002544:Cerebral Infarction / Q000097:blood*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008297:Male; D008875:Middle Aged; D013925:Thromboplastin / Q000032:analysis / Q000502:physiology*
D001779:Blood Coagulation Factors; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D013925:Thromboplastin