[Study on color reaction of copper(II)-neocuprine-glutathione-alcohol system].
The colour reaction of glutathione with copper(II)-neocuproine-alcohol was investigated in the present paper. The absorbance increased distinctly by about 13.8% when alcohol was added to the system. The maximum absorption wavelength lambda max of [Cu (neocuproine)2]+ was at 456 nm and the apparent molar absorptivity was 1.2 x 10(4) L.mol-1.cm-1 in the selected system. Linear dynamic relationship was obtained in the concentration range of 2-24 micrograms.mL-1 for glutathione. The regression equation was as following: A = 0.0104 + 0.0385c. The correlation coefficients was 0.9998. The recoveries obtained were between 98.5% and 99.9%. The relative standard deviations were less than 0.9% for all analyses. This method was used to determine glutathione in soybean extracts by the electromagnetic field technique and satisfactory results were obtained.
Guo
L
L
College of Chemistry, Northeast Normal University, 130024 Changchun.
Liu
G
G
Zhang
Z
Z
Liu
S
S
Wang
L
L
D000970:Antineoplastic Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D013054:Spectrophotometry, Atomic
D000970:Antineoplastic Agents; D010618:Phenanthrolines; D010936:Plant Extracts; D000431:Ethanol; D003300:Copper; C002701:neocuproine; D005978:Glutathione