title: Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10.
abstract: OBJECTIVE
  To investigate the effect of interleukin-10 (IL-10) gene on experimental autoimmune thyroiditis mice.
  
  
  METHODS
  Mice were immunized to induce autoimmune thyroiditis with porcine thyroglobulin (pTg), and thyroids of mice were injected with IL-10 DNA. On d 28 after immunization with pTg, mRNA expression of IL-10 in thyroid glands was detected and thyroid specimens were histopathological studied.
  
  
  RESULTS
  The mRNA expression of IL-10 was detected in thyroid glands on d 7 and 14 after injection of IL-10 plasmid DNA or on COS-7 cells 48 h after IL-10 plasmid DNA transfection. In addition, hIL-10 levels in culture media significantly increased 48 h and 72 h after IL-10 plasmid DNA transfection. Infiltration index of lymphocytes (1.1+/-0.4) in thyroids of IL-10-treated mice was significantly lower than that of pcDNA3-null-treated mice (2.2+/-0.5) (P<0.01). Compared with pcDNA3-null control mice, IL-10-treated mice had lower levels of serum IFN-gamma (P<0.01).
  
  
  CONCLUSIONS
  The direct injection of DNA expression vectors encoding IL-10 into thyroid significantly inhibited development of lymphocytic infiltration of thyroid of autoimmune thyroiditis mice, and alleviated the progression of this disease.
authors.affiliation: Department of Osteoporosis, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
authors.forename: Lu-Yang
authors.initials: LY
authors.lastname: Yu
authors.forename: Shui-Xian
authors.initials: SX
authors.lastname: Shen
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhu
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Wang
authors.forename: Li-He
authors.initials: LH
authors.lastname: Guo
mesh_terms: D000818:Animals; D019556:COS Cells; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013954:Thyroglobulin; D013961:Thyroid Gland / Q000378:metabolism*; D013967:Thyroiditis, Autoimmune / Q000139:chemically induced / Q000628:therapy*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma; D013954:Thyroglobulin