[The T-lymphocyte clonal proliferation in a patient with chronic eosinophilic leukemia].
The gene fingerprinting of T cell receptor beta varial region (TCRbetaV) was used to analyze the T cell clone variations in peripheral blood lymphocytes from a patient with chronic eosinophilic leukemia (CEL) and to find out the characters of CDR3 amino acid sequence related to CEL. RT-PCR was used to amplify betaV1-24 family genes and the gene fingerprinting was set up by denatured polyacrylamide sequence gel electrophoresis. The expanded band was cut and then sequenced. The results showed that one clonal expansion of T cells could be found in TCRbetaV13.2 family. Its nucleotide sequence indicated a monoclonality. The CDR3 amino acid sequence was SFSYEQY and the modif SFSY was special reserved sequence. The other TCRbetaV families remained unchanged. It is concluded that the monoclonal T cells in TCRbetaV13.2 may be related to CEL and it is conjectured that the monoclonal T cells proliferation is induced by CEL malignant cells.
Yang
Yong-Hong
YH
Department of Hematology, The First Hospital, Peking University, Beijing 100034, China.
Li
Hui-Fang
HF
Zhu
Ping
P
D000368:Aged; D000595:Amino Acid Sequence; D002908:Chronic Disease; D022801:Complementarity Determining Regions / Q000737:chemistry; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*
D022801:Complementarity Determining Regions