title: AL-2512, a novel corticosteroid: preclinical assessment of anti-inflammatory and ocular hypertensive effects.
abstract: The anti-inflammatory efficacy and ocular hypertensive effect of AL-2512 were characterized in rodent and feline models of ocular inflammation. Neutrophil influx into ocular tissue following topical ocular administration of test drugs was evaluated in models of endotoxin-induced uveitis. In rats, the anti-inflammatory efficacy of AL-2512 was compared with that of 0.1% dexamethasone. Test drug or vehicle was administered topically before subplantar injection of endotoxin. Neutrophil influx was assessed at 24 hours. Feline eyes, injected intravitreally with endotoxin, were treated topically with 0.1% AL-2512, 1.0% prednisolone acetate or vehicle at various timepoints before and after endotoxin injection. At 12 hours, protein concentration and leukocyte count in aqueous humor were determined. In the feline intraocular pressure (IOP) model, after baseline IOP values were established, AL-2512, dexamethasone, or vehicle was administered topically to both eyes of cats. IOP was measured daily before and during treatment. Topical ocular administration of AL-2512 inhibited endotoxin-induced leukocyte influx in rodent and feline models of uveitis. In rats, AL-2512 significantly inhibited neutrophil influx by 89%, compared with 93% by dexamethasone. In feline eyes, AL-2512 significantly (p < 0.05) inhibited leukocyte infiltration of aqueous humor by 59%, compared to 37% inhibition by prednisolone acetate. Intraocular pressure in cats treated for 32 days with AL-2512 or dexamethasone increased 6% and 18%, respectively. The ocular anti-inflammatory effect of AL-2512 was equivalent to dexamethasone and superior to prednisolone acetate in rat and feline models of ocular inflammation, respectively. This steroid provides anti-inflammatory efficacy equivalent to dexamethasone with a reduced risk of inducing ocular hypertension.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, USA.
authors.forename: Parimal
authors.initials: P
authors.lastname: Bhattacherjee
authors.forename: Partha
authors.initials: P
authors.lastname: Mukhopadhyay
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Paterson
authors.forename: Gustav
authors.initials: G
authors.lastname: Graff
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Gamache
authors.forename: John M
authors.initials: JM
authors.lastname: Yanni
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002415:Cats; D003907:Dexamethasone / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004731:Endotoxins / Q000633:toxicity*; D007322:Instillation, Drug; D009798:Ocular Hypertension / Q000139:chemically induced* / Q000188:drug therapy / Q000517:prevention & control*; D051381:Rats; D011917:Rats, Inbred Lew; D014605:Uveitis / Q000139:chemically induced / Q000188:drug therapy
chemical_list: C480824:21-benzyloxy-9alpha-fluoro-16alpha-methylpregna-1,4-dien-11beta,17alpha-diol-3,20-dione; D000893:Anti-Inflammatory Agents; D004731:Endotoxins; D003907:Dexamethasone