Controlled release of epidermal growth factor (EGF) from EGF-loaded polymeric nanoparticles composed of polystyrene as core and poly(methacrylic acid) as corona in vitro.
Polymeric nanoparticles composed of polystyrene (PS) as core and poly(methacrylic acid) (PMA) as corona were prepared by the dispersion copolymerization. The potential of the nanoparticles as carriers for recombinant human epidermal growth factor (EGF) was investigated. The nanoparticles showed monodispersity and good water-dispersibility. The loading content of EGF to the nanoparticles was very high due to electrostatic interaction between EGF and nanoparticles. EGF was released as a pseudo-zero order pattern after initial burst effect. The nanoparticles were sufficient for A431 cells proliferation.
Park
In-Kyu
IK
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
Seo
Seog-Jin
SJ
Akashi
Mitsuru
M
Akaike
Toshihiro
T
Cho
Chong-Su
CS
D004247:DNA / Q000096:biosynthesis; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D036103:Nanotechnology; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D003692:Delayed-Action Preparations; D011109:Polymethacrylic Acids; D011137:Polystyrenes; C030613:polymethacrylic acid; D004815:Epidermal Growth Factor; D004247:DNA