title: [Fluorescence quenching method for the determination of trace copper with DBH-PF].
abstract: A fluorescence quenching method for the determination of trace Cu(II) was developed. The method was based on the fluorescence quenching effect of DBH-PF and Triton X-100 due to the formation of a non-fluorescent ternary complex in pH 6.2-8.2 KH2PO4-Na2HPO4 medium. The excitation and emission wavelengths are 530 and 560 nm, respectively. The linear range is 0-80 micrograms.L-1 and the detection limit is 0.20 microgram.L-1 for Cu(II). The method has been used to determine trace amounts of Cu(II) in hair, tea and water. The recovery is 98%-104%, and the relative standard deviation is less than 6.9% with satisfactory results.
authors.affiliation: Department of Applied Chemistry, SIBM, 250022 Jinan.
authors.forename: P
authors.initials: P
authors.lastname: Fu
authors.forename: W
authors.initials: W
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Gao
mesh_terms: D003300:Copper / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006846:Hydrocarbons, Halogenated; D013025:Glycine max / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013662:Tea / Q000737:chemistry*
chemical_list: C481353:2,3,7-trihydroxy-9-dibromohydroxyphenylfluorone; D006846:Hydrocarbons, Halogenated; D013662:Tea; D003300:Copper