title: Improved transfection efficiency of cultured human cells.
abstract: We simultaneously tested the transfection efficiency of NT2/D1 and HeLa cells with Lipofectamine (Life Technologies) and Effectene (Qiagen) transfection reagents using the pCH110 eukaryotic assay vector, which contains the lacZ reporter gene. Under our culture conditions for NT2/D1 and HeLa cells, Effectene transfection efficiency could be augmented by simply increasing the amount of plasmid DNA 1.5-3 times above the recommended concentration without any visible cytotoxicity. With the Lipofectamine reagent, optimal transfection efficiency was obtained for both cell lines within the recommended concentrations, but at the top of the range. The results indicate that optimization of the transfection process should include plasmid DNA concentrations above the levels suggested by the manufacturers, in order to accomplish the highest transfection efficiency.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 446, 11001, Serbia and Montenegro, Belgrade, Yugoslavia. gordnik@eunet.yu
authors.forename: Gordana
authors.initials: G
authors.lastname: Nikcevic
authors.forename: Natasa
authors.initials: N
authors.lastname: Kovacevic-Grujicic
authors.forename: Milena
authors.initials: M
authors.lastname: Stevanovic
mesh_terms: D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D008055:Lipids; D010957:Plasmids; D014162:Transfection / Q000379:methods*
chemical_list: D008055:Lipids; C086724:Lipofectamine