Adenine, an inhibitor of platelet aggregation, from the leaves of Cassia alata.
Adenine was isolated as a platelet aggregating inhibitor from the leaves of Cassia alata by HPLC using a triacontylsilyl silica (C(30)) column. The inhibitory effects of adenine and adenosine (positive control) on the platelet aggregation induced by collagen or adenosine 5'-diphosphate (ADP) as an aggregating agent was evaluated with a platelet aggregometer using a laser-scattering method. As a result, the inhibitory effect of adenine was observed in the platelet aggregation induced by collagen (1.0 microg/ml as the final concentration), but little inhibitory effect was noted in the aggregation induced by ADP (5.0 microM as the final concentration), whereas adenosine exhibited potent inhibitory effects on platelet aggregation induced both by collagen and ADP under the same experimental conditions.
Moriyama
Hiroyoshi
H
Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan. fwkp3841@mb.infoweb.ne.jp
Iizuka
Toru
T
Nagai
Masahiro
M
Hoshi
Keiko
K
D000225:Adenine / Q000494:pharmacology*; D000241:Adenosine / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D002366:Cassia / Q000737:chemistry*; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D018515:Plant Leaves / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012542:Scattering, Radiation; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D010975:Platelet Aggregation Inhibitors; D000244:Adenosine Diphosphate; D003094:Collagen; D000225:Adenine; D000241:Adenosine