title: [Determination of tanshinone IIA in rat plasma and the pharmacokinetics by RP-HPLC method].
abstract: OBJECTIVE
  To develop a sensitive and rapid HPLC method for the determination of tanshinone IIA (TS) in rat plasma and to study its pharmacokinetics in rats.
  
  
  METHODS
  TS and 4-chlorodiphenyl (internal standard) were extracted from plasma with ethyl acetate. After liquid-liquid extraction, the sample was analyzed by HPLC with YMC C18 column (5 microns, 150 mm x 3.0 mm ID). The mobile phase consisted of acetontrile-water-acetic acid (74:26:1) at the flow rate of 0.3 mL.min-1, the UV detection wave length was 270 nm.
  
  
  RESULTS
  The calibration curve was linear (r = 0.9981) in the range from 0.05 to 6.40 mg.L-1. The lowest detectable concentration was 0.05 mg.L-1. The recoveries at the concentration of 0.05, 1.60 and 6.40 mg.L-1 were 98.9%, 102.1% and 100.4%, respectively. The inter- and intra-day RSDs were all less than 5%.
  
  
  CONCLUSIONS
  This method is proved to be rapid, precise and reliable enough to be applied to the pharmacokinetics studies of TS in rats after a single dose of 15 mg.kg-1 by oral administration.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Qiao
authors.forename: Pei-ling
authors.initials: PL
authors.lastname: Hou
authors.forename: Ya-wei
authors.initials: YW
authors.lastname: Li
authors.forename: Zeper
authors.initials: Z
authors.lastname: Abliz
mesh_terms: D045784:Abietanes; D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D008297:Male; D010616:Phenanthrenes / Q000097:blood* / Q000493:pharmacokinetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D010616:Phenanthrenes; C021751:tanshinone