title: Moderate disaccharide malabsorption does not affect weight gain and cecal cell proliferation in piglets.
abstract: BACKGROUND
  Intestinal malabsorption of disaccharides or oligosaccharides occurs normally in preterm infants, the majority of the world's adult population when ingesting dairy products, and in sick patients with abnormal intestinal motility or intestinal damage. Although there may be benefit to colonic fermentation, there also could be mucosal damage from excessive fermentation. Moreover, excess fluid in the colon also may impair fermentation. There are few data that address the effects of moderate amounts of carbohydrate, such as 50% of intake, reaching the colon. We addressed the hypothesis that normal weight gain and stool characteristics would occur during disaccharide malabsorption approximating 50% of intake.
  
  
  METHODS
  Twelve piglets (aged 17 days) were randomized to receive control sow milk replacement formula (CON; lactose 60 g/L) or CON modified so that lactulose replaced 50% of the lactose (LAC). During a 5- to 7-day period, weight gain and fecal characteristics were observed before autopsy for cecal histology and determination of cecal cell proliferation using bromodeoxyuridine.
  
  
  RESULTS
  Neither group developed diarrhea. In LAC and CON, formula intakes (g/kg/d) were similar (279 +/- 30 and 280 +/- 35), as were the ratios of weight gain:formula intake (g/mL): 0.23 +/- 0.04 and 0.22 +/- 0.04. Mean cecal cell proliferation was not diminished in the group receiving lactulose (p > .05).
  
  
  CONCLUSIONS
  These data suggest that moderate (50%) disaccharide malabsorption per se does not cause diarrhea and does not impair dietary energy use or colonic cell proliferation.
authors.affiliation: Children's Research Institute, and the Department of Pediatrics, The Ohio State University, Columbus, Ohio, USA. clkien@utmb.edu
authors.forename: C Lawrence
authors.initials: CL
authors.lastname: Kien
authors.forename: James R
authors.initials: JR
authors.lastname: Cooper
authors.forename: Wendy L
authors.initials: WL
authors.lastname: Frankel
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002432:Cecum / Q000166:cytology* / Q000254:growth & development / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D003967:Diarrhea / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D005765:Gastrointestinal Agents / Q000008:administration & dosage / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D007792:Lactulose / Q000008:administration & dosage / Q000378:metabolism*; D011897:Random Allocation; D013552:Swine; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D005765:Gastrointestinal Agents; D007792:Lactulose