Nicotinamide induces both proliferation and differentiation of embryonic stem cells into insulin-producing cells.
In contrast to the consistent observation that methods that promote proliferation also dedifferentiate insulin-producing cells, useful in vitro differentiation protocols must drive both proliferation and differentiation. We herein describe an strategy in which the combination of nutrient restriction and nicotinamide supplementation results in a consistent increase in the mass of insulin-producing cells.
Vaca
P
P
Institute of Bioengineering, University Miguel Hernandez, San Juan de Alicante, Alicante, Spain.
Berná
G
G
Martín
F
F
Soria
B
B
D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D009536:Niacinamide / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007328:Insulin; D009536:Niacinamide