[Influence of environmental factors on the expression of Mycoplasma pneumoniae gene responsible for the synthesis of protein P1 adhesion].
The effect of different physical and chemical factors on the process of transition of persisting mycoplasmas into active virulent state was studied to find out conditions promoting the development of acute infection in the course of chronic infection. The activity of the gene coding the synthesis of protein P1, the main pathogenicity factor, was evaluated in the reverse transcriptase test--PCR under conditions of heat and cold shocks, oxidation stress, varying osmotic pressure. An increased osmotic pressure, heat and cold shocks were shown to induce the transcription activation of the gene coding the synthesis of P1 in the avirulent strain, and consequent restoration of its adhesive properties indicative of virulence. M. pneumoniae avirulent strain was characterized by greater resistance to oxidation stress and to a rise in osmotic pressure, this property requiring further study. Quite probably, M. pneumoniae DNA-binding proteins earlier detected in persisting and avirulent cultures by taking part in the compactization of DNA contribute to the adaptation of mycoplasmas to different stress influences. There are grounds to suggest that M. pneumoniae possess mechanisms regulating the expression of genes, in particular the gene coding the main pathogenicity factor, under the influence of different environmental factors.
Zigangirova
N A
NA
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Barkhatova
O I
OI
Rakovskaia
I V
IV
Gintsburg
A L
AL
D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D003080:Cold Temperature; D005798:Genes, Bacterial; D006358:Hot Temperature; D009177:Mycoplasma pneumoniae / Q000235:genetics* / Q000378:metabolism; D009997:Osmotic Pressure; D018384:Oxidative Stress; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D018829:Adhesins, Bacterial; C035471:adhesin, Mycoplasma pneumoniae
Vliianie faktorov vneshneĭ sredy na ékspressiiu gena Mycoplasma pneumoniae, determiniruiushchego sintez belka adgezii P1.