Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy.
The early detection of carcinoma is very essential for the diagnosis and prognosis of a bladder cancer patient. In this study we have investigated the use of hypericin as a fluorescent tumour marker and laser confocal microscopy as a diagnostic tool to aid the diagnosis of such cancers. Both cellular and clinical studies have been conducted. In the cellular studies, we have compared two bladder cell lines for the uptake and sub-cellular localization of hypericin. It was found that there was a rapid uptake and clearance of hypericin and significant localization in mitochondria and lysosomes. The study also revealed that there was a time-dependent increase in fluorescence intensity in bladder cells. The optimum localization was found to be 2-4 h post drug incubation. In the clinical study, consisting of 30 patients, both white light and fluorescence cystoscopy were performed after hypericin instillation. Biopsies taken from fluorescing regions were imaged using the confocal microscope. The order of fluorescence was detected to be as follows: normal < inflammation < grade 1 < grade 2 < CIS < grade 3. It was also found that the fluorescence intensity increased with the stage of the disease thereby enabling the determination of the degree of invasiveness of cancer. This enables the use of hypericin as a prognostic marker and laser confocal microscopy as a tool to aid in diagnosis of bladder cancer.
Olivo
Malini
M
Division of Medical Sciences, National Cancer Centre, Singapore 169610. dmsmcd@nccs.com.sg
Lau
Weber
W
Manivasager
Vanaja
V
Tan
Puay Hoon
PH
Soo
Khee Chee
KC
Cheng
Christopher
C
D000873:Anthracenes; D002295:Carcinoma, Transitional Cell / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008247:Lysosomes / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D000873:Anthracenes; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D010569:Perylene; C004965:hypericin