Stimulated accumulation of lectin mRNA and stress response in Helianthus tuberosus callus by methyl jasmonate.
Callus from Helianthus tuberosus expresses a mannose-specific lectin (HTA). The level of HTA mRNA significantly increased one hour after treatment of the callus with 20 mg/l methyl jasmonate. Following this, fragmentation of the callus DNA at regular intervals was observed together with strong self-fluorescence emission in the callus cells.
Nakagawa
Ryoji
R
Hokkaido Food Processing Research Center, 589-4 Bunkyodai Midori-Machi, Ebetsu 069-0836, Japan. nakagawa@foodhokkaido.gr.jp
Okumura
Yukihiro
Y
Kawakami
Makoto
M
Yasokawa
Daisuke
D
Nagashima
Koji
K
D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D005453:Fluorescence; D006368:Helianthus / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D037241:Mannose-Binding Lectins; D054883:Oxylipins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D018749:RNA, Plant; C072239:methyl jasmonate