Apoptotic effect of gossypol on human lymphocytes.
The overall objective of this study was to determine the effect of gossypol on human lymphocytes. Blood samples were taken from healthy donors and lymphocytes were cultured with various concentrations of gossypol (25-150 microM). The percentage of apoptotic cells was determined by assessing DNA fragmentation by agarose gel electrophoresis; morphological features of apoptosis were assessed by light microscopy. The concentrations of gossypol required to induce apoptosis in human lymphocytes without causing necrosis through cytotoxic effects were between 25-50 microM.
Yurtcu
Erkan
E
Hematology Laboratory, Gazi University, 06510 Besevler, Ankara, Turkey.
Ergun
Mehmet Ali
MA
Menevse
Adnan
A
D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D006072:Gossypol / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects
D000970:Antineoplastic Agents; D006072:Gossypol