Trypanosoma cruzi: genetic group with peculiar biochemical and biological behavior.
Thirty-two Trypanosoma cruzi strains, isolated from chronic chagasic patients in the northwest of the state of Paran  (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior.
Gomes
Mônica Lúcia
ML
Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maring , PR, 87020-900, Brasil. mlgomes@uem.br
Toledo
Max Jean de Ornelas
MJ
Nakamura
Celso Vataru
CV
Bittencourt
Nilza de Lucas Rodrigues
Nde L
Chiari
Egler
E
de Araújo
Silvana Marques
SM
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D016054:DNA, Protozoan / Q000235:genetics*; D005838:Genotype; D006001:Glycoconjugates / Q000032:analysis; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D009593:Nitroimidazoles / Q000494:pharmacology; D021261:Parasitic Sensitivity Tests; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014344:Trypanocidal Agents / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
D016054:DNA, Protozoan; D006001:Glycoconjugates; D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole