[Expression of elongation factor-1 alpha-A and beta-actin promoters in embryos of transgenic Medaka (Oryzias latipes)].
Two expression vectors with the promoter of either Medaka (Oryzias latipes) elongation factor gene or beta-actin gene were constructed based on pBluescript SK+. Both of them are linked with green-fluorescent protein (GFP) gene. And they are named as pB-EF and pB-BA, respectively. The microinjection experiments were conducted with fertilized Medaka eggs at one-cell stage. The expression of two vectors, pB-EF and pB-BA, was observed under stereo-fluorescence microscope. The detection results showed that both EF-1 alpha-A promoter and beta-actin promoter are strong. In the process of embryo development, the activity of beta-actin promoter became stronger while that of EF-1 alpha-A promoter weaker gradually. beta-actin promoter was but EF-1 alpha-A promoter distributed throughout fish body uniformly. The expression rate of two vectors, pB-EF and pB-BA, are 8.23% and 6.10%, respectively.
Long
Hua
H
Key Laboratory of Freshwater Fish Germplasm Resources & Biotechnology, Ministry of Agriculture, Yangtze River Fisheries Institute, Chinese Academy of Fisheries Sciences, Jingzhou 434000. longhua0000@yahoo.com.cn
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D009990:Oryzias / Q000235:genetics* / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
D000199:Actins; D008164:Luminescent Proteins; D020648:Peptide Elongation Factor 1; D049452:Green Fluorescent Proteins