[Expression of aquaporin - 4 protein in brain from rats with hemorrhagic edema].
OBJECTIVE
To study the expression of aquaporin-4 (AQP4) protein in perihematomal tissue after intracerebral hemorrhage (ICH) in rats.


METHODS
The experimental ICH model was established in rats by injecting quantitative collagenase into the left caudate nuclei of the rats. The changes of brain water content (BWC) were measured by the wet and dry weight methods. Immunohistochemistry was used to determine the change of expression of AQP4 protein during different periods after ICH.


RESULTS
BWC and AQP4 protein expression in perihematomal tissue were increased at 6 hours after ICH and reached peak at 72 hours. In one week following ICH, it was still higher than normal level. In addition, the expression of AQP4 was positively correlated with BWC (r=0.9857, P<0.01).


CONCLUSIONS
AQP4 has a relevant relationship with the formation of brain edema after ICH.
Li
Yan-hua
YH
Department of Neurobiology, Chongqing University of Medical Science, Chongqing 400016, China.
Sun
Shan-quan
SQ
D000818:Animals; D051401:Aquaporin 4; D020346:Aquaporins / Q000032:analysis*; D001921:Brain / Q000378:metabolism*; D001929:Brain Edema / Q000378:metabolism* / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar
C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins