Clinical relevance of matrix metalloproteinase-13 determined with a new highly specific and sensitive ELISA in ascitic fluid of advanced ovarian carcinoma patients.
Matrix metalloproteinases (MMPs) are involved in many physiological and pathophysiological processes, including tumor cell invasion and metastasis. For one member of this family, MMP-13 (collagenase-3), a new, highly specific ELISA with a sensitivity of 0.5 ng MMP-13/ml was established. The protein levels of MMP-13 in ascitic fluids of 30 patients with advanced ovarian cancer FIGO stage III (n = 19) and IV (n = 11) were measured with this ELISA. Using a cut-off value of 0.5 ng MMP-13/mg total protein, two patient subpopulations with short (median 16 months) and long (median 36 months) overall survival were identified. Together with other prognostic markers, determination of MMP-13 in ascitic fluid may help to identify patients at risk for early death and help to individualize adjuvant therapy.
Hantke
Bernd
B
Department of Chemistry, Biochemistry I, University of Bielefeld, D-33615 Bielefeld, Germany.
Harbeck
Nadia
N
Schmalfeldt
Barbara
B
Claes
Ingeborg
I
Hiller
Oliver
O
Luther
Marc-Oliver
MO
Welk
Anita
A
Kuhn
Walther
W
Schmitt
Manfred
M
Tschesche
Harald
H
Muehlenweg
Bernd
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001202:Ascitic Fluid / Q000201:enzymology* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D017364:Collagenases / Q000032:analysis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D053509:Matrix Metalloproteinase 13; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology; D012680:Sensitivity and Specificity; D015996:Survival Rate
D000911:Antibodies, Monoclonal; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse