Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase.
Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg-->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp-->Asn mutation, corresponding to the ammonium binding site, in the second mutant. The phenotypic changes in these mutants are discussed in relation to their genotypes.
Van Dommelen
Anne
A
Center of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium.
Keijers
Veerle
V
Wollebrants
An
A
Vanderleyden
Jozef
J
D019943:Amino Acid Substitution; D001216:Asparagine; D001224:Aspartic Acid; D015806:Azospirillum brasilense / Q000201:enzymology / Q000235:genetics*; D001665:Binding Sites; D005798:Genes, Bacterial / Q000235:genetics*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D001224:Aspartic Acid; D001216:Asparagine; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase