Activity and heavy metal resistance of non-specific esterases in leaf beetle Chrysomela lapponica from polluted and unpolluted habitats.
We compared the general activity and heavy metal resistance of non-specific esterases in two populations of the leaf beetle Chrysomela lapponica from habitats severely contaminated by heavy metals (mostly Ni and Cu) and two populations from unpolluted habitats. Concentrations of Ni and Cu in adult beetles from the most polluted site were 7.7 and 3.6 times higher that in beetles from unpolluted habitats. Larval esterases showed higher activity and lower susceptibility to heavy metals than esterases of adults. Larval esterase activity did not differ between populations from polluted and unpolluted sites, but adult beetles from polluted localities had lower esterase activity than beetles from unpolluted habitats. Both Cu and Ni sulfates in millimolar concentrations in vitro suppressed esterase activity of larvae from unpolluted habitats, but caused no negative effect on esterases of larvae from polluted sites. Similarly, inhibition of adult esterase activity by Ni was stronger in beetles from unpolluted localities than in beetles from polluted localities. This indicates that resistance of non-specific esterases to heavy metals is higher in leaf beetle populations from contaminated environment.
Zvereva
Elena
E
Section of Ecology, Department of Biology, University of Turku, Turku FIN-20014, Finland. elena.zvereva@utu.fi
Serebrov
Valery
V
Glupov
Viktor
V
Dubovskiy
Ivan
I
D000704:Analysis of Variance; D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D003300:Copper / Q000032:analysis / Q000506:poisoning*; D004777:Environment; D004785:Environmental Pollutants / Q000032:analysis / Q000506:poisoning*; D004791:Enzyme Inhibitors / Q000032:analysis / Q000506:poisoning; D004950:Esterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007814:Larva / Q000201:enzymology; D009532:Nickel / Q000032:analysis / Q000506:poisoning*
D004785:Environmental Pollutants; D004791:Enzyme Inhibitors; D003300:Copper; D009532:Nickel; D004950:Esterases