title: Effect of amino acid sequence and pH on nanofiber formation of self-assembling peptides EAK16-II and EAK16-IV.
abstract: Atomic force microscopy (AFM) and axisymmetric drop shape analysis-profile (ASDA-P) were used to investigate the mechanism of self-assembly of peptides. The peptides chosen consisted of 16 alternating hydrophobic and hydrophilic amino acids, where the hydrophilic residues possess alternating negative and positive charges. Two types of peptides, AEAEAKAKAEAEAKAK (EAK16-II) and AEAEAEAEAKAKAKAK (EAK16-IV), were investigated in terms of nanostructure formation through self-assembly. The experimental results, which focused on the effects of the amino acid sequence and pH, show that the nanostructures formed by the peptides are dependent on the amino acid sequence and the pH of the solution. For pH conditions around neutrality, one of the peptides used in this study, EAK16-IV, forms globular assemblies and has lower surface tension at air-water interfaces than another peptide, EAK16-II, which forms fibrillar assemblies at the same pH. When the pH is lowered below 6.5 or raised above 7.5, there is a transition from globular to fibrillar structures for EAK16-IV, but EAK16-II does not show any structural transition. Surface tension measurements using ADSA-P showed different surface activities of peptides at air-water interfaces. EAK16-II does not show a significant difference in surface tension for the pH range between 4 and 9. However, EAK16-IV shows a noticeable decrease in surface tension at pH around neutrality, indicating that the formation of globular assemblies is related to the molecular hydrophobicity.
authors.affiliation: Department of Chemical Engineering, University of Waterloo, 200 University Avenue, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Yooseong
authors.initials: Y
authors.lastname: Hong
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Legge
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000686:Amyloidosis; D019281:Dimerization; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D009842:Oligopeptides / Q000737:chemistry*; D012996:Solutions; D013500:Surface Tension
chemical_list: C098329:EAK16 peptide; D009842:Oligopeptides; D012996:Solutions