[Effects of polysaccharides of Cryptoporus volvatus on bronchial hyperreasponsiveness and inflammatory cells in ovalbumin sensitized rats].
To study the effects of polysaccharides of cultured Cryptoporus volvatus(CVPS) on airway hyperresponsiveness of ovalbumin-sensitized rats and to evaluate their mechanisms.



Polysaccharides A, B (5mg/kg, 20mg/kg) and ketotifen(5mg/kg) or vehicle(same volume of saline) were administrated orally for 10 days in ovalbumin  -sensitized rats, methacholine bronchial provocation tests were performed to determine airway hyperresponsiveness. Bronchoalveolar lavage fluid (BALF) and peritoneal lavage fluid were prepared after the animals were challenged by nebulized antigen. The differential white cell count in BALF,and the degranulated mast cell count as well as differential white cell count in peritoneal lavage fluid were performed.



Polysaccharides markedly inhibited the increased lung resistance and the decreased lung compliance induced by antigen challenge,significantly reduced total cell counts and absolute eosinophil counts in BALF(P<0.05); polysaccharides B was more effective than polysaccharides A. They also inhibited recruitment of inflammatory cells in peritoneal lavage fluid and inhibited the allergen-induced mast cell degranulation.



Polysaccharides of CVPS inhibit airway hyperresponsiveness by stabilizing mast cell membranes and reducing infiltration and chemotaxis of eosinophils and may be developed as a potential anti-asthmatic drug.
Tang
Hui-fang
HF
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Ji-qiang
JQ
Xie
Qiang-min
QM
Zhao
Xiao-yan
XY
Ke
Chuan-kuei
CK
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D016535:Bronchial Hyperreactivity / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015550:Cell Degranulation / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology*; D011124:Polyporaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018927:Anti-Asthmatic Agents; D011134:Polysaccharides; D010047:Ovalbumin