Organogenesis of heart-vascular system derived from mouse 2 cell stage embryos and from early embryonic stem cells in vitro.
Regenerative medical treatment with embryonic stem cells (an ES cell) is a goal for organ transplantation. Structures that are tubular in nature (i.e. blood capillaries) were induced from early embryonic stem (EES) cells in vitro using embryotrophic factor (ETFs). In addition, cardiac muscle cells could be identified as well. However, differentiation of EES cells into a complete cardiovascular system was difficult because 3 germ layer primordial organs are directed embryologically in various ways and it is not possible to guide only cardiovascular organs. Thus, we introduced ETFs after the formation of an embryoid body and were successful in cloning cell clusters that beat, thus deriving only cardiovascular organs. The application of this to the treatment of various cardiovascular diseases is promising.
Ishiwata
Isamu
I
Ishiwata Obstet Gynecologic Hosp, Mito-shi, Ibaraki-ken, Japan.
Tamagawa
Tomoharu
T
Tokieda
Yuko
Y
Iguchi
Megumi
M
Sato
Kahei
K
Ishikawa
Hiroshi
H
D000818:Animals; D002319:Cardiovascular System / Q000196:embryology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002999:Clone Cells; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D038081:Organogenesis; D013234:Stem Cells / Q000166:cytology*