title: Quantitative elimination of Flavokavines A and B from Kava Kava (Piper methysticum G. Forst) by isoelectric focused adsorptive bubble separation.
abstract: Adsorptive bubble separation, though still rarely used, is a suitable method for enrichment of surface-active macromolecules such as enzymes and proteins. There is a lack of investigations with small molecules, which can also be separated from complex mixtures by this method. In this work, an aqueous extract of Kava Kava ( Piper methysticum G. Forst) was used as a model system. Enrichment of undesirable Flavokavine A (7) and Flavokavine B (8) in the foam was influenced by the pH value, the amount of saponin as surface active substance, and the flow rate of the foam-forming gas. Efficiency was highest with diluted samples at pH 6.5. Under these conditions, transfer of Kavapyrone (1-6) to the foam was negligible.
authors.affiliation: Department of Chemical Technical Analysis and Chemical Food Technology, Technical University of Munich, Weihenstephaner Steig 23, 85354 Freising, Germany.
authors.forename: Marlène
authors.initials: M
authors.lastname: Backleh
authors.forename: Perihan
authors.initials: P
authors.lastname: Ekici
authors.forename: Günther
authors.initials: G
authors.lastname: Leupold
authors.forename: Harun
authors.initials: H
authors.lastname: Parlar
mesh_terms: D000327:Adsorption; D006254:Hawaii; D007525:Isoelectric Focusing / Q000379:methods; D020901:Kava / Q000737:chemistry*; D015394:Molecular Structure; D009512:New Guinea; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D011753:Pyrones