Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury.
To characterize the features of JAK/STAT signaling in Drosophila immune response, we have identified totA as a gene that is regulated by the JAK/STAT pathway in response to septic injury. We show that septic injury triggers the hemocyte-specific expression of upd3, a gene encoding a novel Upd-like cytokine that is necessary for the JAK/STAT-dependent activation of totA in the Drosophila counterpart of the mammalian liver, the fat body. In addition, we demonstrate that totA activation also requires the NF-KB-like Relish pathway, indicating that fat body cells integrate the activity of NF-KB and JAK/STAT signaling pathways upon immune response. This study reveals that, in addition to the pattern recognition receptor-mediated NF-KB-dependent immune response, Drosophila undergoes a complex systemic response that is mediated by the production of cytokines in blood cells, a process that is similar to the acute phase response in mammals.
Agaisse
Hervé
H
Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA. agaisse@rascal.med.harvard.edu
Petersen
Ulla Maja
UM
Boutros
Michael
M
Mathey-Prevot
Bernard
B
Perrimon
Norbert
N
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D001952:Bridged-Ring Compounds / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007239:Infections; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D019076:Transgenes
D001952:Bridged-Ring Compounds; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; C101626:Rel protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; C444499:dome protein, Drosophila; D011505:Protein-Tyrosine Kinases; C010349:tetramethylenedisulfotetramine