title: [Effects of BIO-1211 on eosinophil chemotaxis, recruitment and mediator release].
abstract: To study the effects of very late antigen(VLA) antagonist BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.
  
  
  
  Eosinophil chemotaxis was induced by platelet activating factor(PAF) in vitro and eosinophil recruitment and release were determined in vivo.
  
  
  
  VLA antagonist BIO-1211 inhibited eosinophil chemotaxis induced by PAF. The inhibitory rates at 4x10(-11), 4x10(-10), 4x10(-9) mol x L(-1) were 24.9%, 29.9%, and 31.3%, respectively. Pretreatment by BIO-1211 1, 3 and 10 mg x kg(-1) intraperitoneally inhibited the recruitment of eosinophils in PAF in the rat induced by Sephadex in a dose dependent manner. Inhibitory rates were 60.3%, 68.9%, and 72.9%(P<0.05), respectively. BIO-1211 did not inhibit eosinophil peroxidase(EPO) release from eosinophils.
  
  
  
  BIO-1211 inhibits eosinophil chemotaxis and recruitment, alleviates local inflammation, and may represent a new type of drug for allergic diseases.
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhao
authors.forename: Ji-qiang
authors.initials: JQ
authors.lastname: Chen
authors.forename: Qiang-min
authors.initials: QM
authors.lastname: Xie
authors.forename: Hui-fang
authors.initials: HF
authors.lastname: Tang
authors.forename: Ru-lain
authors.initials: RL
authors.lastname: Bian
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000187:drug effects* / Q000502:physiology; D039041:Integrin alpha4beta1 / Q000037:antagonists & inhibitors* / Q000502:physiology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D039041:Integrin alpha4beta1; D009842:Oligopeptides; D010972:Platelet Activating Factor; C118154:BIO 1211; D047209:Eosinophil Peroxidase; D010544:Peroxidases