[Effect of eucalyptus globulus oil on activation of nuclear factor-kappaB in THP-1 cells].
To study the effect of eucalyptus globulus oil on the activity of nuclear factor-kappaB(NF-kappaB) in THP-1 cell line.



THP-1 cells were cultured with or without eucalyptus globulus oil at different concentrations (1, 10, 100 mg x L(-1), 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg x L(-1), 30 min). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by indirect immunofluorescence and laser scanning confocal microscope. The expression of NF-kappaB/p65 in nuclei was measured by Western-blot analysis.



The FITC-label NF-kappaB/p65 was mainly located in the nuclei after THP-1 cells were stimulated with LPS. Whereas, no fluorescence were seen in the nuclei of cells pretreated with eucalyptus globulus oil. This effect on NF-kappaB/p65 nuclear translocation was in a concentration dependent manner.



Eucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells.
Zhou
Jian-ya
JY
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Tang
Fa-di
FD
Mao
Guo-gen
GG
Shao
Jie
J
Wang
Yan
Y
Bian
Ru-lian
RL
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005052:Eucalyptus / Q000737:chemistry*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D008070:Lipopolysaccharides; D016328:NF-kappa B; D010938:Plant Oils; D014107:Tosyllysine Chloromethyl Ketone