Effects of sodium magnesium fructose diphosphate on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome.
OBJECTIVE
To study the effects of sodium magnesium fructose diphosphate (SMFD) on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome, so as to explore the protective mechanisms of SMFD on cerebral ischemia.


METHODS
The synaptosomes from normal rat brain were prepared by phase partition and cultured with oxygen-glucose deprivation to establish ischemic synaptosome model. The intrasynaptosomal free calcium concentration and nitric oxide synthase activity were detected separately after the synaptosomes were co-incubated with SMFD (1.3 mmol.L-1) or fructose-1, 6-diphosphate (FDP, 4.0 mmol.L-1) for 60 min.


RESULTS
SMFD decreased the free calcium concentration and reduced the activity of nitric oxide synthase (NOS) of ischemic synaptosomes. Its effects were more powerful than those of FDP.


CONCLUSIONS
SMFD may protect neurons from ischemic injury by preventing intracellular Ca2+ overload and inhibiting the activity of nitric oxide synthase.
Zeng
Fan-xin
FX
Department of Pharmacology and Toxicology, Chongqing Pharmaceutical Research Institute, Chongqing 400061, China. zfx2806@sina.com
Dong
Zhi
Z
Zhou
Qi-xin
QX
D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology; D005635:Fructosediphosphates / Q000494:pharmacology*; D008274:Magnesium / Q000737:chemistry; D008297:Male; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000737:chemistry; D013574:Synaptosomes / Q000378:metabolism*
D002614:Chelating Agents; D005635:Fructosediphosphates; D012964:Sodium; D019001:Nitric Oxide Synthase; D008274:Magnesium; C029063:fructose-1,6-diphosphate; D002118:Calcium