Endocytosis at the synaptic terminal.
Exocytosis of neurotransmitter from a synaptic vesicle is followed by efficient retrieval of its constituent membrane and proteins. Real-time measurements indicate that fast and slow modes of retrieval operate in parallel at a number of presynaptic terminals. Two mechanisms can be distinguished by electron microscopy: clathrin-mediated retrieval of small vesicles and bulk retrieval of large cisternae. Methods that investigate the behaviour of individual vesicles have recently demonstrated a third route of retrieval: the rapid reversal of a pore-like connection between the vesicle and surface ('kiss-and-run'). Key aims for the future are to identify the molecules underlying different mechanisms of endocytosis at the synapse and the signals that select between them.
Royle
Stephen J
SJ
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Lagnado
Leon
L
D000818:Animals; D002966:Clathrin / Q000502:physiology; D034281:Dynamins / Q000502:physiology; D004705:Endocytosis / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology; D007700:Kinetics; D008854:Microscopy, Electron; D008959:Models, Neurological; D009469:Neuromuscular Junction / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology* / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology
D002966:Clathrin; D034281:Dynamins