title: Diamond paste based immunosensor for the determination of azidothymidine.
abstract: An amperometric immunosensor, based on diamond paste (diamond powder and paraffin oil), has been constructed for the assay of azidothymidine (AZT). The diamond paste is impregnated with anti-AZT. The immunosensor can be used reliably for the assay of azidothymidine in its pharmaceutical formulations. The potential used for azidothymidine assay was + 240 mV vs. Ag/AgCl electrode. The surface of the immunosensor can be regenerated by simply polishing, thereby obtaining fresh immunocomposite ready to be used in a new assay. The new amperometric immunosensor, based on diamond paste, gives reliable results for the assay of AZT as raw material and from its pharmaceutical formulation.
authors.affiliation: Department of Chemistry, University of Pretoria, Pretoria, South Africa. Raluca.stefan@chem.up.ac.za
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan
authors.forename: Rahel Girmai
authors.initials: RG
authors.lastname: Bokretsion
mesh_terms: D018130:Diamond; D004563:Electrochemistry; D004566:Electrodes; D007118:Immunoassay / Q000379:methods*; D015215:Zidovudine / Q000032:analysis* / Q000276:immunology
chemical_list: D015215:Zidovudine; D018130:Diamond