title: Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase.
abstract: Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg-->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp-->Asn mutation, corresponding to the ammonium binding site, in the second mutant. The phenotypic changes in these mutants are discussed in relation to their genotypes.
authors.affiliation: Center of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Van Dommelen
authors.forename: Veerle
authors.initials: V
authors.lastname: Keijers
authors.forename: An
authors.initials: A
authors.lastname: Wollebrants
authors.forename: Jozef
authors.initials: J
authors.lastname: Vanderleyden
mesh_terms: D019943:Amino Acid Substitution; D001216:Asparagine; D001224:Aspartic Acid; D015806:Azospirillum brasilense / Q000201:enzymology / Q000235:genetics*; D001665:Binding Sites; D005798:Genes, Bacterial / Q000235:genetics*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D001224:Aspartic Acid; D001216:Asparagine; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase