[Tests of some peptides isolated from residue after insulin isolation from the pancreas. II. Establishment of heterogeneity level].
Heterogeneity of the active substance of Lipotropin-Polfa preparation was investigated by the method of gel column chromatography. The isolated polypeptide hormone has been found to possess the average molecular weight nine times smaller than that reported in the literature. This polypeptide was separated into six fractions by chromatography on Sephadex G-10. These fractions were found to differ in respect of the qualitative and quantitative amino acid composition. With the purpose of comparison, an analogous study of Lipormone-Labor. Choay (France) was performed. Similarity of this preparation to fraction I of Lipotropin-Polfa has been shown.
Jarzebiński
J
J
Zakład Analizy Leków Instytutu Nauki o Leku Akademii Medycznej w Warszawie, ul. Banacha 1, 02-097 Warszawa.
Szrajber
Z
Z
Krasnodebska
B
B
D002850:Chromatography, Gel; D008970:Molecular Weight; D010179:Pancreas / Q000737:chemistry*; D008083:beta-Lipotropin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*
D008083:beta-Lipotropin
Badania niektórych peptydów wyodrebnionych z pozostałości po izolowaniu insuliny z trzustki. II. Ustalanie stopnia heterogeniczności.