Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi.
A cellular role and the mechanism of action for small GTPase Arl1 have been defined. Arl1-GTP interacts with the GRIP domains of Golgin-97 and Golgin-245, a process dependent on conserved residues of the GRIP domains that are important for Golgi targeting. The switch II region of Arl1 confers the specificity of this interaction. Arl1-GTP mediates Golgi recruitment of Golgin-97 in a switch II-dependent manner, whereas tethering Arl1-GTP onto endosomes can mediate endosomal targeting of Golgin-97. Golgin-97 and Golgin-245 are dissociated from the Golgi when Arl1 is knocked-down by its siRNA. Arl1-GTP thus functions to recruit Golgin-97 and Golgin-245 onto the Golgi via interacting with their GRIP domains.
Lu
Lei
L
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore.
Hong
Wanjin
W
D020727:ADP-Ribosylation Factors / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D000077614:Golgi Matrix Proteins; D006801:Humans; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D020798:Two-Hybrid System Techniques
D001324:Autoantigens; D000077614:Golgi Matrix Proteins; C450721:Golgi complex autoantigen, 97-kDa; D008565:Membrane Proteins; D010455:Peptides; D034741:RNA, Small Interfering; C042459:p 230; C097313:ADP-ribosylation factor related proteins; D020727:ADP-Ribosylation Factors