title: Investigation of biological risk in mycobacteriology laboratories: a multicentre study.
abstract: METHODS
  Microbiology laboratories constitute a special working environment that may pose a risk of infectious disease to persons in or near them.
  
  
  OBJECTIVE
  To assess whether clinical mycobacteriology laboratories in Spanish hospitals comply with occupational health and safety guidelines, and if laboratory workers are protected against the risk of exposure to biological agents in the workplace.
  
  
  METHODS
  A transversal epidemiological study was carried out in 26 hospitals from October to December 2000 by means of a standardised survey questionnaire to evaluate the workers, workload, training and information and safety practices in mycobacteria laboratories.
  
  
  RESULTS
  Less than half of employees receive periodic information on the health hazards involved in their work. More than a third of the workers in mycobacteriology laboratories stated that the laboratories lack effective air filtering systems and more than half stated that negative pressurisation was not maintained in the work area. Biosafety masks are used by little more than half of the workers. The most frequently used Biological Safety Cabinets are Class IIB.
  
  
  CONCLUSIONS
  Our findings demonstrate that clinical microbiology laboratories in Spanish hospitals comply poorly with the prevention measures outlined in the applicable directives and regulations.
authors.affiliation: Mycobacteria Reference Laboratory, University of Cordoba, Cordoba, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Vaquero
authors.forename: P
authors.initials: P
authors.lastname: Gómez
authors.forename: M
authors.initials: M
authors.lastname: Romero
authors.forename: M J
authors.initials: MJ
authors.lastname: Casal
mesh_terms: D000328:Adult; D016021:Epidemiologic Studies; D005260:Female; D019983:Guideline Adherence; D006801:Humans; D017053:Infection Control; D007753:Laboratories; D008297:Male; D017056:Medical Laboratory Personnel; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D016273:Occupational Exposure; D012134:Respiratory Protective Devices; D012307:Risk Factors; D012449:Safety; D013030:Spain; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control* / Q000635:transmission*; D014691:Ventilation; D017132:Workplace