title: Cell-derived sequences in the N-terminal region of the polyprotein of a cytopathogenic pestivirus.
abstract: Efficient proteolytic release of nonstructural protein 3 (NS3) from the viral polyprotein is considered to be crucial for the cytopathogenicity of pestiviruses. Here we describe a novel cytopathogenic (cp) bovine viral diarrhea virus strain (BVDV CP8) with a complex insertion composed of viral and cell-derived sequences, including two fragments of the cellular J-domain protein Jiv (J-domain protein interacting with viral protein) located in the N-terminal region of the polyprotein. BVDV CP8 expresses a Jiv fusion protein of 513 amino acids in addition to a complete set of viral proteins. This protein has the capacity to induce NS2-3 cleavage in trans. Accordingly, CP8 is a representative of a novel type of cp pestivirus with a cp-specific mutation located outside of the NS2-3 gene.
authors.affiliation: Institut für Virologie (Fachbereich Veterinärmedizin), Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Müller
authors.forename: G
authors.initials: G
authors.lastname: Rinck
authors.forename: H-J
authors.initials: HJ
authors.lastname: Thiel
authors.forename: N
authors.initials: N
authors.lastname: Tautz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D020815:Polyproteins / Q000737:chemistry* / Q000502:physiology; D020365:RNA Helicases; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000502:physiology; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D010447:Peptide Hydrolases; D020815:Polyproteins; D020365:RNA Helicases; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C074956:p125 protein, bovine diarrhea virus; C086730:p80 protein, bovine viral diarrhea virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases