Triazinic herbicide determination by gas chromatography-mass spectrometry in breast milk.
A solid-phase extraction procedure using a graphitized carbon black cartridge for extraction and cleaning of a series of five triazines (atrazine, deethylatrazine, deisopropylatrazine, ametryne and prometryne) from breast milk samples was developed. Using a chemometric methodology, the optimisation of both the analysis time and the triazinic herbicide separation by gas chromatography-mass spectrometry (GC-MS) was then carried out with only 18 experiments. Detection and quantification limits for 1ml breast milk sample were, respectively, 0.3 and 1 ppb for each studied compound. The variation coefficients were less than 5% over the concentration range from 1 to 100 ppb. The accuracy was between 98.63 and 104.62% for each triazinic herbicide. The recovery was between 58.64 and 63.22% for the concentration range from 1 to 100 ppb for each triazinic herbicide. The assay was successfully applied to the analysis of several breast milk samples.
Balduini
L
L
Equipe Sciences Séparatives et Biopharmaceutiques, UFR des Sciences Médicales et Pharmaceutiques, 25030 Cedex, Besançon, France.
Matoga
M
M
Cavalli
E
E
Seilles
E
E
Riethmuller
D
D
Thomassin
M
M
Guillaume
Y C
YC
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014227:Triazines
D006540:Herbicides; D014227:Triazines