In vitro regulation of beta1 and beta3 integrin subunits in endometrial epithelial cells from normal endometrium.
OBJECTIVE
To evaluate the effect of prostaglandin E2 (PGE2) and interleukin-1beta (IL-1beta) on integrin expression. DESIGN Cultures of endometrial epithelial cells from normal endometrium.


METHODS
All endometrial specimens were obtained from the Obstetrics and gynecology Department of the Catholic University, Rome, Italy.


METHODS
Four patients were normal menstrual cycles undergoing operative laparoscopy for non-endometrial problems.


METHODS
Endometrial samples were collected by uterine courettings.


METHODS
Immunocytochemistry for beta1 and beta3 integrin subunits.


RESULTS
PGE2 clearly enhances both beta1 and beta3 integrin subunit expression. IL-1beta seem to slightly increase only beta3 subunit expression.


CONCLUSIONS
In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE2 is also involved in local paracrine regulation of integrin expression.
Pierro
Emilia
E
Unità Operativa di Ginecologia ed Ostetricia, Ospedale G.B. Grassi, Roma, Italy
Minici
Francesca
F
Alesiani
Ornella
O
Monica
Loredana D
LD
Anna
Maria M
MM
Muncuso
Salvatore
S
Lanzone
Antonio
A
D002478:Cells, Cultured; D003470:Culture Media; D015232:Dinoprostone / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology
D003470:Culture Media; D019012:Integrin beta1; D039661:Integrin beta3; D007375:Interleukin-1; D015232:Dinoprostone