title: Antibody response in the female rabbit reproductive tract to influenza haemagglutinin encoded by a recombinant myxoma virus.
abstract: The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisations. HA IgG was also detected in reproductive tract fluids but was only about 1% the titer of that in serum. HA IgA was not detected in serum of any infected groups and was occasionally detected in reproductive tract fluids at a low titer only after infections through mucosal sites. HA IgM was also detected only in some of the reproductive tract fluids at very low levels. Induction of ovulation did not change these patterns and B cell homing to the reproductive tract was not profound. In contrast, HA IgG and IgM titers in ovarian follicular fluids were comparable to that in serum. These data suggest that if this virus is used to deliver an immunocontraceptive vaccine that requires a high-level antibody response, the target antigen needs to be accessible to serum antibody or in the ovary.
authors.affiliation: Division of Biochemistry and Molecular Biology, the School of Life Science, The Australian National University, Canberra, Australia. wgu@cicr.uq.edu.au
authors.forename: Wenyi
authors.initials: W
authors.lastname: Gu
authors.forename: Michael
authors.initials: M
authors.lastname: Holland
authors.forename: Peter
authors.initials: P
authors.lastname: Janssens
authors.forename: Peter
authors.initials: P
authors.lastname: Kerr
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D003269:Contraception, Immunologic; D005260:Female; D015571:Follicular Fluid / Q000276:immunology; D005822:Genetic Vectors; D005836:Genitalia, Female / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis; D023421:Models, Animal; D009233:Myxoma virus / Q000235:genetics / Q000378:metabolism; D009975:Orthomyxoviridae / Q000276:immunology*; D010060:Ovulation; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines