Cholesteryl glucoside-induced protection against gastric ulcer.
The cytoprotective effect of heat shock proteins (HSPs) promises new therapeutic modalities for medical treatment. We examined the anti-ulcer effect of cholesteryl glucoside (1-O-cholesteryl-beta-D-glucopyranoside, CG) on cold-restraint stress-induced gastric ulcer in rats, in terms of its correlative ability to activate heat shock factor (HSF) and to induce HSP70. Rapid induction of CG occurred in animal tissues, especially in stomach, after exposure to stress, indicating that this glycolipid might act as an anti-stress, lipid mediator involved in the very early stages of stress-induced signal transduction. Orally administered CG apparently showed anti-ulcer activity in rats via HSF activation and HSP70 induction. When compared with geranylgeranylacetone (GGA), the well known as an effective, synthetic anti-ulcer agent, CG proved to have the same level of strength on ulcer inhibition. GGA caused CG and HSP70 induction in gastric mucosa, indicating that GGA induced HSP70 via CG production. CG thus might be useful for medical treatment of stress-induced diseases, and as an anti-stress supplement for daily diet.
Kunimoto
Shohko
S
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo 112-8610, Japan.
Murofushi
Wataru
W
Yamatsu
Isao
I
Hasegawa
Yukie
Y
Sasaki
Narie
N
Kobayashi
Susumu
S
Kobayashi
Tetsuyuki
T
Murofushi
Hiromu
H
Murakami-Murofushi
Kimiko
K
D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism* / Q000627:therapeutic use; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000627:therapeutic use; D004224:Diterpenes / Q000378:metabolism / Q000627:therapeutic use; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000188:drug therapy / Q000378:metabolism*; D013315:Stress, Psychological; D013696:Temperature
D000897:Anti-Ulcer Agents; D004224:Diterpenes; D018840:HSP70 Heat-Shock Proteins; C027578:cholesteryl glucoside; D002784:Cholesterol; C031049:geranylgeranylacetone