Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants.
To investigate UV light response mechanisms in higher plants, we isolated a UV light-sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. These results indicate that UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.
Sakamoto
Ayako
A
Department of Ion-Beam-Applied Biology, Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan. sakamoto@taka.jaeri.go.jp
Lan
Vo Thi Thuong
VT
Hase
Yoshihiro
Y
Shikazono
Naoya
N
Matsunaga
Tsukasa
T
Tanaka
Atsushi
A
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D020134:Catalytic Domain / Q000235:genetics; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D003624:Darkness; D005720:Gamma Rays; D008027:Light; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D029681:Arabidopsis Proteins; D016685:Mitomycin; C425929:DNA polymerase zeta; C479807:AtREV3 protein, Arabidopsis; D004259:DNA-Directed DNA Polymerase