Functional split and crosslinking of the membrane domain of the beta subunit of proton-translocating transhydrogenase from Escherichia coli.
Proton pumping nicotinamide nucleotide transhydrogenase from Escherichia coli contains an alpha subunit with the NAD(H)-binding domain I and a beta subunit with the NADP(H)-binding domain III. The membrane domain (domain II) harbors the proton channel and is made up of the hydrophobic parts of the alpha and beta subunits. The interface in domain II between the alpha and the beta subunits has previously been investigated by cross-linking loops connecting the four transmembrane helices in the alpha subunit and loops connecting the nine transmembrane helices in the beta subunit. However, to investigate the organization of the nine transmembrane helices in the beta subunit, a split was introduced by creating a stop codon in the loop connecting transmembrane helices 9 and 10 by a single mutagenesis step, utilizing an existing downstream start codon. The resulting enzyme was composed of the wild-type alpha subunit and the two new peptides beta1 and beta2. As compared to other split membrane proteins, the new transhydrogenase was remarkably active and catalyzed activities for the reduction of 3-acetylpyridine-NAD(+) by NADPH, the cyclic reduction of 3-acetylpyridine-NAD(+) by NADH (mediated by bound NADP(H)), and proton pumping, amounting to about 50-107% of the corresponding wild-type activities. These high activities suggest that the alpha subunit was normally folded, followed by a concerted folding of beta1 + beta2. Cross-linking of a betaS105C-betaS237C double cysteine mutant in the functional split cysteine-free background, followed by SDS-PAGE analysis, showed that helices 9, 13, and 14 were in close proximity. This is the first time that cross-linking between helices in the same beta subunit has been demonstrated.
Althage
Magnus
M
Department of Biochemistry and Biophysics, Göteborg University, Sweden.
Karlsson
Jenny
J
Gourdon
Pontus
P
Levin
Mikael
M
Bill
Roslyn M
RM
Tigerström
Anna
A
Rydström
Jan
J
D002384:Catalysis; D003062:Codon; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D015951:Factor Xa / Q000737:chemistry; D007700:Kinetics; D008954:Models, Biological; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009243:NAD / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D009250:NADP Transhydrogenases / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011510:Proteolipids / Q000737:chemistry; D011522:Protons; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D003062:Codon; D003432:Cross-Linking Reagents; D010455:Peptides; D011510:Proteolipids; D011522:Protons; C020485:proteoliposomes; D009243:NAD; D009249:NADP; D009250:NADP Transhydrogenases; D014357:Trypsin; D015951:Factor Xa; D003545:Cysteine