Soybean-derived phosphatidylinositol recovers amyloid beta protein-induced neurotoxicity in cultured rat hippocampal neurons.
Effects of soybean-derived phosphatidylinositol (PI) on amyloid beta protein (10 nM Abeta(25-35))-induced changes in Cl(-)-ATPase activity, intracellular Cl- concentration ([Cl-]i) and glutamate neurotoxicity were examined using cultured rat hippocampal neurons. Soybean-derived PI (> or =5 nM) dose-dependently recovered Abeta-induced decrease in neuronal Cl(-)-ATPase activity without any changes in the activities of Na+,K(+)-ATPase and anion-insensitive Mg(2+)-ATPase. Soybean-derived PI reduced Abeta-induced elevation of [Cl-](i) as assayed using a Cl(-)-sensitive fluorescent dye, and prevented Abeta-induced aggravation of glutamate neurotoxicity assayed by mitochondrial 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt reducing activity and plasma membrane lactate dehydrogenase release. These data suggest that soybean-derived PI may be useful as a therapeutic and/or preventive strategy for Alzheimer's disease.
Zhang
Nan-Yan
NY
Department of Pharmacology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8506, Japan.
Kitagawa
Kaori
K
Wu
Bo
B
Inagaki
Chiyoko
C
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010716:Phosphatidylinositols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max
D016229:Amyloid beta-Peptides; D010716:Phosphatidylinositols; D000251:Adenosine Triphosphatases