Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
Hybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were An. minimus species A: V form (X1,Y1), M form (X2,Y1); species C: P form (X3,Y2). The results of reciprocal and back crosses indicated that the two morphologically variant forms of species A were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes, whereas they were genetically incompatible with species C and/or the P form. Hybrid progeny was only obtained from both forms of species A females x species C males, but asynaptic salivary gland polytene chromosomes on 3L and partial development of ovarian follicles in females were seen. Back crosses of F1 hybrid males with parental species A females provided viable progeny, while back crosses of F1 hybrid females with parental species C males provided progeny of low viability and adult males with abnormal spermatozoa, suggesting the partial reproductive isolation of An. minimus species A and C.
Choochote
Wej
W
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. wchoocho@mail.med.cmu.ac.th
Rongsriyam
Yupha
Y
Leemingsawat
Somjai
S
Jitpakdi
Atchariya
A
Komalamisra
Narumon
N
Surathin
Kamhaeng
K
Somboon
Pradya
P
Chen
Bin
B
Wongkamchai
Sirijit
S
Jariyapan
Narissara
N
Tippawangkosol
Pongsri
P
Pitasawat
Benjawan
B
Riyong
Doungrat
D
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics / Q000502:physiology*; D005260:Female; D006824:Hybridization, Genetic; D007621:Karyotyping; D008297:Male; D013045:Species Specificity; D013785:Thailand