Construction and characterisation of a full-length infectious molecular clone from a fast replicating, X4-tropic HIV-1 CRF02.AG primary isolate.
Based on our previous analysis of HIV-1 isolates from Cameroon, we constructed a full-length infectious molecular clone from a primary isolate belonging to the CRF02.AG group of recombinant viruses which dominate the HIV-epidemic in West and Central Africa. The virus derived by transfection of the proviral clone pBD6-15 replicated with similar efficiency compared to its parental isolate and used CXCR4 as coreceptor as well. Furthermore, HIV-1 BD6-15 exhibited similar replication properties and virus yield as the reference B-type HIV-1 strain NL4-3. Sequence analysis revealed open reading frames for all structural and accessory genes apart from vpr. Phylogenetic and bootscanning analyses confirmed that BD6-15 clusters with CRF02.AG recombinant strains from West and Central Africa with similar cross-over points as described for the CRF02.AG prototype strain lbNG. Thus, pBD6-15 represents the first non-subtype B infectious molecular clone of a fast replicating, high producer, X4-tropic primary HIV-1 isolate, which had only been briefly passaged in primary cells.
Tebit
Denis M
DM
Abteilung Virologie, Universität Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany.
Zekeng
Léopold
L
Kaptué
Lazare
L
Kräusslich
Hans-Georg
HG
Herchenröder
Ottmar
O
D000328:Adult; D036022:Capsid Proteins / Q000096:biosynthesis; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D015683:Gene Products, gag / Q000096:biosynthesis; D016679:Genome, Viral; D006023:Glycoproteins / Q000096:biosynthesis; D019427:HIV Integrase / Q000096:biosynthesis; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000096:biosynthesis; D011533:Proviruses / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014162:Transfection; D014776:Virus Cultivation; D014779:Virus Replication
D036022:Capsid Proteins; D015683:Gene Products, gag; D006023:Glycoproteins; D011498:Protein Precursors; D019718:Receptors, CXCR4; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; C023587:protein M (glycoprotein); D019427:HIV Integrase