The metabolic conversion of 9,11,13-eleostearic acid (18:3) to 9,11-conjugated linoleic acid (18:2) in the rat.
The presence of a significant amount of 9,11-conjugated linoleic acid (CLA, 9,11-18:2) was confirmed in the liver and plasma lipids of rats fed a 1% (w/w % of diet) eleostearic acid (ESA, 9,11,13-18:3) diet for 4 wk. The chemical structure of the 9,11-CLA apparent in the tissue lipids was identified by gas chromatography-electron impact mass spectrometry after its conversion to a 4,4-dimethyloxazoline derivative. The concentration of CLA in the total fatty acids of the liver and plasma lipids reached to about 1%, for each in the CLA-supplemented rats, while reaching 3.2% and 2.5%, respectively, in the ESA-supplemented rats. The results suggest that alpha-ESA is metabolized partially to CLA via a delta 13-saturation reaction in the rat. Some biological activities observed in alpha-ESA-fed animals may be ascribed also to CLA that is formed from ESA in the body.
Tsuzuki
Tsuyoshi
T
Laboratory of Food & Biodynamic Chemistry, Graduate School of Life Science and Agriculture, Tohoku University, Sendai 981-8555, Japan.
Igarashi
Miki
M
Komai
Michio
M
Miyazawa
Teruo
T
D000818:Animals; D002849:Chromatography, Gas; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D008042:Linolenic Acids / Q000008:administration & dosage / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010080:Oxazoles / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D044243:Linoleic Acids, Conjugated; D008042:Linolenic Acids; D010080:Oxazoles; C004934:eleostearic acid; C046938:9,11-linoleic acid; C057549:4,4-dimethyloxazoline