title: Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test.
abstract: The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.
authors.affiliation: Faculty of Dentistry, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. lsassone@montreal.com.br
authors.forename: Luciana Moura
authors.initials: LM
authors.lastname: Sassone
authors.forename: Rivail Antonio Sergio
authors.initials: RA
authors.lastname: Fidel
authors.forename: Sandra Rivera
authors.initials: SR
authors.lastname: Fidel
authors.forename: Marina
authors.initials: M
authors.lastname: Dias
authors.forename: Raphael Júnior
authors.initials: RJ
authors.lastname: Hirata
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002710:Chlorhexidine / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D012973:Sodium Hypochlorite / Q000008:administration & dosage*; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002710:Chlorhexidine