[Testing of some dibenzoazepines and dibenzocycloheptadiene derivatives with high pressure thin layer chromatography].
An influence of several adsorbents and the composition of the eluent mixtures on the separation of dibenzoazepine and dibenzocycloheptadiene derivatives was examined by thin-layer chromatography. The best system (RP-18 and methanol-buffer solution pH 8.5 19:1) was employed for the separation of amitryptiline and doxepine by HPLC. Both compounds were determined in ng amounts in pharmaceutical preparations and in blood by addition and in patients blood. Doxepine was used as internal standard for the determination of amitryptiline.
Szumiło
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Kameda Chemii Nieorganicznej i Analitycznej Akademii Medycznej w Lublinie, ul. Okopowa 1, 20-022 Lublin.
Przyborowska
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Przyborowski
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Misztal
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D000639:Amitriptyline / Q000097:blood / Q000302:isolation & purification*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods*; D003986:Dibenzocycloheptenes / Q000097:blood / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D004316:Doxepin / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration
D002021:Buffers; D003986:Dibenzocycloheptenes; D004316:Doxepin; D000639:Amitriptyline; C000914:dibenzocycloheptadiene
Badania niektórych pochodnych dibenzoazepiny i dibenzocykloheptadienu metoda chromatografii cienkowarstwoweji wysokosprawnej cieczowej.