Fluorescence properties, thermal duplex stability, and kinetics of formation of cyanin platinum DNAs.
Fluorescent and haptenized, monofunctionally binding platinum compounds are increasingly used for chemically labeling nucleic acids for hybridization detection purposes. Here we analyze in detail the effect of labeling density of the cyanin-3 and -5 platinum DNA adducts on fluorescence and thermal stability. We also analyzed the kinetics of the reaction of the cyanin platinum compounds with DNA. The data provided are important for the design of optimal platinum DNA labeling and hybridization conditions for fluorescence hybridization applications.
van de Rijke
Frans M
FM
Department Molecular Cell Biology, Leiden University Medical Centre, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
Heetebrij
Robert J
RJ
Talman
Eduard G
EG
Tanke
Hans J
HJ
Raap
Anton K
AK
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004048:Diethylamines / Q000737:chemistry; D004140:Dinitrophenols / Q000737:chemistry; D005453:Fluorescence; D007700:Kinetics; D051379:Mice; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010984:Platinum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D002232:Carbocyanines; D004048:Diethylamines; D004140:Dinitrophenols; C089187:cyanine dye 3; C085321:cyanine dye 5; D010984:Platinum; D004247:DNA