title: Oral immunization with rotavirus VP7 expressed in transgenic potatoes induced high titers of mucosal neutralizing IgA.
abstract: Rotaviruses (RV) are a common cause of severe diarrhea in young children, resulting in nearly one million deaths worldwide annually. Rotavirus VP7 was the rotavirus neutralizing protein. Previous study reported that VP7 DNA vaccine can induce high levels of IgG in mice but cannot protect mice against challenge (Choi, A.H., Basu, M., Rae, M.N., McNeal, M.M., Ward, R.L., 1998. Virology 250, 230-240). We found that rotavirus VP7 could maintain its neutralizing immunity when it was transformed into the potato genome. Mice immunized with the transformed tubers successfully elicited serum IgG and mucosal IgA specific for VP7. The mucosal IgA titer was as high as 1000, while serum IgG titer was only 600. Neutralizing assays indicated that IgA could neutralize rotavirus. These results indicate the potential usefulness of plants for production and delivery of edible rotavirus vaccines.
authors.affiliation: Institute of Immunology, Third Military Medical University, District Shapingba, Chongqing, People's Republic of China.
authors.forename: Yu-Zhang
authors.initials: YZ
authors.lastname: Wu
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Li
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Mou
authors.forename: Lei
authors.initials: L
authors.lastname: Fei
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Miao
authors.initials: M
authors.lastname: Geng
authors.forename: Zheng-Cai
authors.initials: ZC
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Zou
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005243:Feces / Q000821:virology; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology* / Q000821:virology; D051379:Mice; D009500:Neutralization Tests; D030821:Plants, Genetically Modified / Q000378:metabolism; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control; D022243:Rotavirus Vaccines / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D022462:Vaccines, Edible / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; C050779:VP7 protein, Rotavirus; D022462:Vaccines, Edible