title: Expression of heat shock protein (Hsp) 70 and Hsp 40 in gastric cancer.
abstract: Heat shock proteins (Hsp) 70 and Hsp 40 are stress proteins that cooperate as chaperones in mammalian cells. We determined the expression of Hsp 70 and Hsp 40 in 81 gastric cancers. Immunoreactivities to Hsp 70 and Hsp 40 were detected in 67.9 and 22.2% of tumors, respectively. Immunohistochemical analysis showed enhanced Hsp 70 and Hsp 40 expression in gastric tumor tissue, relative to the surrounding normal tissue. Overexpression of Hsp 70 and Hsp 40 was also confirmed by immunoblotting. Among various clinicopathological parameters, low histopathological differentiation was associated with reduced expression of both proteins.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. hhisomot@net.nagasaki-u.ac.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Isomoto
authors.forename: Mikio
authors.initials: M
authors.lastname: Oka
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Yano
authors.forename: Yusei
authors.initials: Y
authors.lastname: Kanazawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Soda
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Terada
authors.forename: Toru
authors.initials: T
authors.lastname: Yasutake
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakayama
authors.forename: Saburo
authors.initials: S
authors.lastname: Shikuwa
authors.forename: Fuminao
authors.initials: F
authors.lastname: Takeshima
authors.forename: Heiichiro
authors.initials: H
authors.lastname: Udono
authors.forename: Ikuo
authors.initials: I
authors.lastname: Murata
authors.forename: Kenzo
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000473:pathology* / Q000601:surgery
chemical_list: D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins