Relationship among location of T-antigen-induced DNA distortion, auxiliary sequences, and DNA replication efficiency.
T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or two copies of the 21-bp repeat. In a construct with enhanced replication efficiency, bending occurred in a 69-bp cellular sequence located upstream of a single copy of the 21-bp repeat. Bending occurred both upstream of ori and in the three 21-bp repeats located downstream of ori in a construct with reduced replication efficiency. In a construct with no 21-bp repeats, DNA distortion occurred downstream of ori. The results indicate that SV40 DNA replication is enhanced when the structure of the regulatory region allows the DNA to form a bent structure upstream of the initial movement of the replication fork.
Okuley
Susan
S
Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
Call
Mindy
M
Mitchell
Tara
T
Hu
Bugen
B
Woodworth
Mary E
ME
D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011196:Potassium Permanganate / Q000494:pharmacology; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D011196:Potassium Permanganate