A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.
Doménech
N
N
Unidad de Investigación, Complejo Hospitalario Juan Canalejo, 15006 A Coruña, Spain. ndomenech@canalejo.org
Alvarez
B
B
Bullido
R
R
Alonso
F
F
Ezquerra
A
A
Domínguez
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D015153:Blotting, Western; D018956:CD5 Antigens / Q000096:biosynthesis* / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D000939:Epitopes; D005434:Flow Cytometry; D006023:Glycoproteins / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D011233:Precipitin Tests; D011487:Protein Conformation; D013552:Swine
D000911:Antibodies, Monoclonal; D018956:CD5 Antigens; D003432:Cross-Linking Reagents; D000939:Epitopes; D006023:Glycoproteins