An economical adaptation of the RosetteSep procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells.
This study reports an economical adaptation of the RosetteSep procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45x10(6) PBMC with 45x10(8) Alsevers' stored red blood cells (RBC) in 1 ml requires 50 microl of RosetteSep bifunctional antibody cocktail and provides NK cells of 99% purity with average yields of 43%.
Warren
Hilary S
HS
Division of Immunology and Genetics, John Curtin School of Medical Research, Australian National University, PO Box 334, Canberra, ACT 2601, Australia. Hilary.Warren@amu.edu.au
Rana
Purna M
PM
D001793:Blood Preservation; D002469:Cell Separation / Q000191:economics / Q000379:methods*; D015925:Cryopreservation; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D012397:Rosette Formation