Developmental acquisition of sensory transduction in hair cells of the mouse inner ear.
Sensory transduction in hair cells requires assembly of membrane-bound transduction channels, extracellular tip-links and intracellular adaptation motors with sufficient precision to confer nanometer displacement sensitivity. Here we present evidence based on FM1-43 fluorescence, scanning electron microscopy and RT-PCR that these three essential elements are acquired concurrently between embryonic day 16 and 17, several days after the appearance of hair bundles, and that their acquisition coincides with the onset of mechanotransduction.
Géléoc
Gwénaëlle S G
GS
Department of Neuroscience and Department of Otolaryngology, University of Virginia School of Medicine, Box 801392, Charlottesville, Virginia 22908, USA.
Holt
Jeffrey R
JR
D000818:Animals; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure; D005333:Fetus; D005456:Fluorescent Dyes; D006198:Hair Cells, Auditory / Q000196:embryology* / Q000502:physiology* / Q000648:ultrastructure; D006309:Hearing / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology / Q000648:ultrastructure; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008959:Models, Neurological; D020409:Molecular Motor Proteins / Q000502:physiology; D024461:Myosin Type I; D009218:Myosins / Q000378:metabolism
D005456:Fluorescent Dyes; D007473:Ion Channels; D020409:Molecular Motor Proteins; D024461:Myosin Type I; C460367:MYO1C protein, human; D009218:Myosins