title: Apolipoprotein E -491 promoter polymorphism is an independent risk factor for Alzheimer's disease in the Chinese population.
abstract: Polymorphisms at positions -491, -427 and -219 in the promoter region of the Apolipoprotein E APOE gene have been variously reported to confer an increased risk of developing Alzheimer's disease (AD) independent of the effect of epsilon 2, 3 or 4 alleles in exon 4. In order to assess APOE promoter polymorphisms as independent risk factors in AD we have compared results in 183 definite or probable AD cases with 133 controls. We assayed markers at sites -491, -427, -219, and +113 in APOE gene and a polymorphic Hha1 site in the nearby APOC1 gene. We found that APOE promoter polymorphisms and APOC1 insertion alleles were significantly associated with AD. However, after stratification for epsilon 4 allele, only the A allele at -491 in APOE remained significantly associated with AD. The effects of the other markers depended almost entirely upon linkage disequilibrium with epsilon 4 allele, and only trends remained when cases and controls were stratified for the presence or absence of epsilon 4 allele. This occurred irrespective of whether markers were examined separately or together as haplotypes. So in the Chinese population only APOE -491 promoter alleles confer significant risk of AD independent of epsilon 4 status.
authors.affiliation: Shanghai Research Centre of Life Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: J D
authors.initials: JD
authors.lastname: Yang
authors.forename: G Y
authors.initials: GY
authors.lastname: Feng
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Cheung
authors.forename: D
authors.initials: D
authors.lastname: St  Clair
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ichimura
mesh_terms: D000368:Aged; D000409:Alanine / Q000235:genetics; D000483:Alleles; D000544:Alzheimer Disease / Q000453:epidemiology / Q000235:genetics*; D001057:Apolipoproteins E / Q000145:classification / Q000235:genetics*; D044466:Asian People; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015331:Cohort Studies; D004252:DNA Mutational Analysis; D039721:Diagnostic and Statistical Manual of Mental Disorders; D005091:Exons; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D013912:Threonine / Q000235:genetics
chemical_list: D001057:Apolipoproteins E; D013912:Threonine; D000409:Alanine