title: Inter-individual variability in levels of human microsomal protein and hepatocellularity per gram of liver.
abstract: OBJECTIVE
  To determine levels of microsomal protein (MPPGL) and hepatocellularity (HPGL) per gram of human liver and their interindividual variability.
  
  
  METHODS
  Triplicate liver samples were used to determine values of MPPGL (n = 20) and HPGL (n = 7) after accounting for the fractional loss of microsomal protein or hepatocytes during processing. Repeated measurements from each liver sample allowed the estimation of true interindividual variability in MPPGL and HPGL using ANOVA.
  
  
  RESULTS
  The value of MPPGL ranged from 26 to 54 mg g(-1) (mean(geo)= 33 mg g(-1)). The value of HPGL ranged from 65 to 185 x 10(6) cells g(-1) (mean(geo)= 10(7) x 10(6) cells g(-1)).
  
  
  CONCLUSIONS
  There is significant interindividual variability in MPPGL, which has implications for the accurate extrapolation of in vitro data on drug metabolism to predict in vivo metabolic clearance.
authors.affiliation: Molecular Pharmacology and Pharmacogenetics, Division of Clinical Sciences (South), The University of Sheffield, Sheffield, UK.
authors.forename: Z E
authors.initials: ZE
authors.lastname: Wilson
authors.forename: A
authors.initials: A
authors.lastname: Rostami-Hodjegan
authors.forename: J L
authors.initials: JL
authors.lastname: Burn
authors.forename: A
authors.initials: A
authors.lastname: Tooley
authors.forename: J
authors.initials: J
authors.lastname: Boyle
authors.forename: S W
authors.initials: SW
authors.lastname: Ellis
authors.forename: G T
authors.initials: GT
authors.lastname: Tucker
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008875:Middle Aged; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins; D003577:Cytochrome P-450 Enzyme System