Molecular and physiological characterization of Italian isolates of Pyrenochaeta lycopersici.
Corky root of tomato caused by Pyrenochaeta lycopersici is a disease of concern in Italy and for many tomato growing areas in the world. Isolates of the fungus were characterized at both the physiological and molecular level. The optimal in vitro growth temperature for all isolates was 23 degrees C. All Italian isolates of P. lycopersici showed similar RAPD and esterase banding patterns. No relevant polymorphisms were detected after enzymatic digestion of PCR-amplified ITS and IGS regions. The overall results indicate a low degree of genetic variability within a collection of 43 Italian isolates. These data are of interest in breeding programs for resistance against corky root of tomato and they provide useful information for the development of molecular diagnostic tools for the rapid identification and detection of P. lycopersici.
Infantino
Alessandro
A
Istituto Sperimentale per la Patologia Vegetale, Via C.G. Bertero 22, 00156 Roma, Italy. a.infantino@ispave.it
Aragona
Maria
M
Brunetti
Angela
A
Lahoz
Ernesto
E
Oliva
Anna
A
Porta-Puglia
Angelo
A
D004271:DNA, Fungal / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D004950:Esterases / Q000378:metabolism; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D007558:Italy; D018551:Solanum lycopersicum / Q000382:microbiology*; D016533:Mycological Typing Techniques; D010935:Plant Diseases / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D004950:Esterases