Detection of a gene cluster that is dispensable for human herpesvirus 6 replication and latency.
The U3-U7 gene cluster of human herpesvirus 6 (HHV-6) was replaced with an enhanced green fluorescent protein-puromycin gene cassette containing the cytomegalovirus major immediate-early promoter. Neither viral replication in T cells nor latency and reactivation in macrophages was impaired. During HHV-6 latency, the cytomegalovirus promoter used the transcription start sites employed in cytomegalovirus latency.
Kondo
Kazuhiro
K
Department of Microbiology, The Jikei University School of Medicine, Minato-ku, Tokyo, 105-8461, Japan. kkondo@jikei.ac.jp
Nozaki
Hideo
H
Shimada
Kazuya
K
Yamanishi
Koichi
K
D003587:Cytomegalovirus / Q000235:genetics; D017781:Genes, Immediate-Early; D006367:HeLa Cells; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D014775:Virus Activation; D017735:Virus Latency; D014779:Virus Replication