Induction of infectious petunia vein clearing (pararetro) virus from endogenous provirus in petunia.
Infection by an endogenous pararetrovirus using forms of both episomal and chromosomal origin has been demonstrated and characterized, together with evidence that petunia vein clearing virus (PVCV) is a constituent of the Petunia hybrida genome. Our findings allow comparative and direct analysis of horizontally and vertically transmitted virus forms and demonstrate their infectivity using biolistic transformation of a provirus-free petunia species. Some integrants within the genome of P.hybrida are arranged in tandem, allowing direct release of virus by transcription. In addition to known inducers of endogenous pararetroviruses, such as genome hybridization, tissue culture and abiotic stresses, we observed activation of PVCV after wounding. Our data also support the hypothesis that the host plant uses DNA methylation to control the endogenous pararetrovirus.
Richert-Pöggeler
Katja R
KR
Friedrich Miescher Institute, PO Box 2543, CH-4002 Basel, Switzerland. richert@fmi.ch
Noreen
Faiza
F
Schwarzacher
Trude
T
Harper
Glyn
G
Hohn
Thomas
T
D001483:Base Sequence; D019470:Biolistics; D019175:DNA Methylation; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D016366:Open Reading Frames; D032306:Petunia / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D011533:Proviruses / Q000502:physiology; D012190:Retroviridae / Q000235:genetics*; D016662:Virus Integration
D017931:DNA Primers; D004279:DNA, Viral