Svi3: A provirus common integration site in c-myc in SL/Kh pre-B lymphomas.
Spontaneous pre-B lymphomas in SL/Kh mice occur by somatic acquisition of a provirus genome of endogenous murine leukemia virus (MuLV). Inverse PCR amplification and sequence analyses of a provirus and its host flanking fragment revealed a proviral insertion into c-myc in 3 out of 60 SL/Kh pre-B lymphomas, named Svi3 lymphomas (SL/Kh virus integration site-3). Southern blot analysis revealed that two lymphomas had clonal integration in c-myc exon 1 and the other, in the promoter region. In 2 out of 3 Svi3 lymphomas, a fusion transcript of provirus 3' long terminal repeat and c-myc and a normal full-length c-myc transcript were obtained, but in one Svi3 lymphoma, only the normal transcript was obtained. All three Svi3 lymphomas had increased c-myc expression, producing normal 67-kDa c-Myc protein. Svi3 lymphomas had more mature phenotypes in the steps of early B-cell differentiation than Svi1 lymphomas, in which c-myc expression was indirectly up-regulated by provirus integration into Stat5a.
Jin
Guang
G
Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Tsuruyama
Tatsuaki
T
Yamada
Yoshihiro
Y
Hiai
Hiroshi
H
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D016259:Genes, myc / Q000235:genetics*; D007438:Introns; D009052:Leukemia Virus, Murine / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000821:virology; D051379:Mice; D008815:Mice, Inbred Strains; D008894:Milk Proteins; D041681:NIH 3T3 Cells; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D016662:Virus Integration
D020413:3' Untranslated Regions; D017931:DNA Primers; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D008894:Milk Proteins; D050799:STAT5 Transcription Factor; C494093:Stat5a protein, mouse; D015534:Trans-Activators