title: Haemoglobin biosynthesis site in rabbit embryo erythroid cells.
abstract: Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, with erythroid cells obtained from rabbit embryos, in order to compare these results with those of animals treated with saponine or phenylhydrazine. Our results are similar to those obtained in assays with adult mammals, birds, amphibians, reptiles and fish, after induction of haemolytic anaemia. Therefore, the treatment did not interfere with the process studied, confirming our previous findings. Immunoelectron microscopy showed no labelling of mitochondria or other cellular organelles supposedly involved in the final biosynthesis of haemoglobin molecules, suggesting instead that it occurs free in the cytoplasm immediately after the liberation of haeme from the mitochondria, by electrostatic attraction between haeme and globin chains.
authors.affiliation: Laboratório de Genética, Instituto Butantan, Avenida Vital Brazil 1500, 05503-900 São Paulo-SP, Brazil. amcianciarullo@hotmail.com
authors.forename: Aurora M
authors.initials: AM
authors.lastname: Cianciarullo
authors.forename: Alvaro L
authors.initials: AL
authors.lastname: Bertho
authors.forename: Maurilio J
authors.initials: MJ
authors.lastname: Soares
authors.forename: Tânia M
authors.initials: TM
authors.lastname: Hosoda
authors.forename: Simone
authors.initials: S
authors.lastname: Nogueira-Silva
authors.forename: Willy
authors.initials: W
authors.lastname: Beçak
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology*; D004906:Erythrocyte Count; D041905:Erythroid Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D006454:Hemoglobins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D011817:Rabbits
chemical_list: D006454:Hemoglobins