In situ detection of antigen-specific tumor-infiltrating lymphocytes using newly designed tetramers.
We described a new process for the design of HLA tetramers using soluble MHC class I molecules purified from Epstein Barr Virus-transformed B cells. This method does not rely on genetic engineering and presents a significant advantage in view of the polymorphism of MHC class I molecules because tetramers can be produced with any HLA molecule. Here, we showed that our HLA-A*0201 tetramers provided experimental results similar to those obtained with tetramers made with recombinant MHC molecules. Moreover, they can be used to efficiently identify peptide-specific T cells from ex vivo PBMCs as well as from lymphocytes infiltrating human tumor. This innovative and simple method could be widely adopted, specially in diagnostic procedures for monitoring peptide-based immunotherapy.
Bras-Gonçalves
Rachel
R
Département d'Immunologie, Inserm U567,CNRS UMR 8104, Université Paris V René Descartes, IFR Alfred Jost, Institut Cochin, 27 rue du Faubourg Saint Jacques 75014 Paris, France.
Le Gal
Frédérique-Anne
FA
Avril
Marie-Françoise
MF
Dalod
Marc
M
Connan
Francine
F
Chaboissier
Aude
A
Choppin
Jeannine
J
Guillet
Jean-Gérard
JG
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D002460:Cell Line; D015658:HIV Infections / Q000276:immunology; D015234:HLA-A Antigens / Q000737:chemistry* / Q000302:isolation & purification; D015789:HLA-A2 Antigen; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008545:Melanoma / Q000276:immunology; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology; D020836:Protein Structure, Quaternary; D013601:T-Lymphocytes / Q000276:immunology
D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D009842:Oligopeptides