title: cDNA cloning and transcript distribution of two novel members of the neuroparsin family in the desert locust, Schistocerca gregaria.
abstract: This study describes the identification and distribution of two novel neuroparsin precursor transcripts (Scg-NPP3/Scg-NPP4) in the desert locust, Schistocerca gregaria. Unlike Scg-NPP1 and Scg-NPP2, both transcripts were not only detected in the brain, but also in various other tissues, such as fat body, ventral nerve cord, testis and male accessory glands. Northern analysis showed that the levels of these transcripts are regulated during larval development, as well as during moulting and reproductive cycles. A significant increase in both mRNAs was observed during the period that just precedes the initial sexual activity of adult females and males. In silico analysis of sequence databases revealed the existence of several other neuroparsin-like peptides in a variety of arthropod species, including crustaceans and chelicerates. Neuroparsins also display similarities with vertebrate IGFBP.
authors.affiliation: Laboratory of Developmental Physiology and Molecular Biology, Department of Animal Physiology and Neurobiology, K.U. Leuven, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Claeys
authors.forename: G
authors.initials: G
authors.lastname: Simonet
authors.forename: T
authors.initials: T
authors.lastname: Van Loy
authors.forename: A
authors.initials: A
authors.lastname: De Loof
authors.forename: J
authors.initials: J
authors.lastname: Vanden Broeck
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics*; D006110:Grasshoppers / Q000235:genetics* / Q000254:growth & development*; D007301:Insect Hormones / Q000235:genetics*; D008018:Life Cycle Stages / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012098:Reproduction / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D007301:Insect Hormones