title: [Platelet phospholipase A2 mRNA content changes and cDNA cloning in rat blood with bacteria infection].
abstract: OBJECTIVE
  To explore the changes of rat platelet phospholipase A2 (PLA2) mRNA content in bacteria infected rat and study the cDNA and amino acid sequences of the PLA2 structure to lay a good foundation for the development of new antibiotics.
  
  
  METHODS
  The PLA2 mRNA level in blood was determined by RT-PCR. The DNA sequence was cloned and analyzed.
  
  
  RESULTS
  After injection of bacteria in rats, the mRNA level of PLA2 in blood increased markedly. The cDNA and amino acid sequence were highly homologous to other PLA2 cDNA from different tissues of the rat.
  
  
  CONCLUSIONS
  Platelet PLA2 in blood responded quickly to bacteria infection in gene level. Therefore, the PLA2 protein was produced increasingly which was shown to control the infection with bacteria. Although there are little difference between PLA2 cDNA cloned from blood and other sources in DNA and amino acid sequences, the catalytic site for enzymatic activity and basic structure are identical.
authors.affiliation: Department of Pharmacology, Affiliated Tumor Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Tao-tao
authors.initials: TT
authors.lastname: Liu
authors.forename: Ning-sheng
authors.initials: NS
authors.lastname: Liang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Zi-qing
authors.initials: ZQ
authors.lastname: Meng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000097:blood*; D013211:Staphylococcus aureus
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D010741:Phospholipases A; D054467:Phospholipases A2