title: Injected TFF1 and TFF3 bind to TFF2-immunoreactive cells in the gastrointestinal tract in rats.
abstract: Peptides of the trefoil factor family (TFF1, TFF2 and TFF3) are co-secreted with mucus in most organ systems and are believed to interact with mucins to produce high-viscosity, stable gel complexes. We have previously demonstrated that cells in the GI tract possess binding sites to TFF2 and that injected TFF2 ends up in the mucus layer. In the present study, tissue binding and metabolism of parenterally administered human TFF1 and TFF3 in rats were described and compared to the immunohistochemical localization of the TFF peptides. 125I-TFF1 monomer and 125I-TFF3 mono- and dimer were given intravenously to female Wistar rats. The tissue distribution was assessed by gamma counting of organ samples and by autoradiography of histological sections. The degradation of 125I-TFF3 was studied by means of trichloracetic acid (TCA) precipitation and the saturability of the binding by administration of excess unlabelled peptide. The TFF peptides were localized in histologic sections from the GI tract by immunohistochemistry. Injected TFF3 dimer (12%) was taken up by the GI tract. At autoradiography, grains were localized to the same cells that were immunoreactive to TFF2. The binding could be displaced by excess TFF3. Similar binding was observed for the TFF1 and TFF3 monomers apart from binding in the stomach, where the uptake was only 15% in comparison to the dimer. There was no specific binding outside the GI tract and no binding to TFF1 or TFF3 immunoreactive cells. In conclusion, the TFF2-binding cells in the gastrointestinal tract seem to have basolateral, receptor-like activity to all three TFF peptides. The mucous neck cells of the stomach predominantly take up TFFs with two trefoil domains, indicating a different receptor-like activity in the stomach compared to the rest of the GI tract.
authors.affiliation: Anatomy Department B, The Panum Institute, University of Copenhagen, 3 Blegdamsvej, 2200 N, Copenhagen, Denmark. s.s.poulson@mai.ku.dk
authors.forename: S S
authors.initials: SS
authors.lastname: Poulsen
authors.forename: J
authors.initials: J
authors.lastname: Thulesen
authors.forename: B
authors.initials: B
authors.lastname: Hartmann
authors.forename: H L
authors.initials: HL
authors.lastname: Kissow
authors.forename: E
authors.initials: E
authors.lastname: Nexø
authors.forename: L
authors.initials: L
authors.lastname: Thim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004064:Digestive System / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D009077:Mucins / Q000008:administration & dosage / Q000378:metabolism*; D009124:Muscle Proteins / Q000008:administration & dosage / Q000378:metabolism*; D009479:Neuropeptides; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism*; D011506:Proteins / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D025521:Tumor Suppressor Proteins
chemical_list: D004967:Estrogens; D006131:Growth Inhibitors; D007457:Iodine Radioisotopes; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; C051838:TFF1 protein, human; C000604007:TFF2 protein, human; C082495:TFF3 protein, human; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D025521:Tumor Suppressor Proteins