Determination of mirtazapine in human plasma by liquid chromatography.
A rapid high-performance liquid chromatographic method for the quantitation of mirtazapine in human plasma is presented. The method is based on a liquid-liquid extraction and reversed-phase chromatography with fluorimetric detection. The separation was performed on a Luna microm C(18)(2) 50 x 4.6 mm I.D. column using an isocratic elution. Zolpidem hemitartrate was used as the internal standard. The between-day precision expressed by relative standard deviation was less than 5% and inaccuracy does not exceed 6%. A low limit of quantitation (1.5 ng/ml) and a short time of analysis (4 min) makes this assay suitable for pharmacokinetic studies.
Ptácek
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P
Pharmakl s.r.o., U vojenské nemocnice 1200, CZ-16200 Prague 6, Czech Republic. pharmakl@mbox.vol.cz
Klíma
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J
Macek
J
J
D000929:Antidepressive Agents, Tricyclic / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D006801:Humans; D008297:Male; D008803:Mianserin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D000078785:Mirtazapine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000929:Antidepressive Agents, Tricyclic; D008803:Mianserin; D000078785:Mirtazapine