title: Using wound fluid analyses to identify trace element requirements for efficient healing.
abstract: A series of wound fluid and blood plasma samples from 20 patients with breast cancer were analysed by Potentiometric Stripping Analysis and computer-aided chemical speciation to quantify the concentrations of the trace elements of copper and zinc in the samples and to investigate the individual species of copper and zinc present. Comparisons were made between total concentrations of copper and zinc in wound fluid, pre-operative blood plasma levels and reference values. A wound fluid model constructed using JESS identified the main copper and zinc species present. It was also used to investigate the effects of a change in pH and changes in the total concentrations of certain components on their predominance. The clinical significance of the research is discussed, together with suggestions for a continuation in the research.
authors.affiliation: Chemistry Department, Cardiff University, Wales, UK.
authors.forename: P W
authors.initials: PW
authors.lastname: Jones
authors.forename: D M
authors.initials: DM
authors.lastname: Taylor
authors.forename: D R
authors.initials: DR
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Finney
authors.forename: A
authors.initials: A
authors.lastname: Iorwerth
authors.forename: D
authors.initials: D
authors.lastname: Webster
authors.forename: K G
authors.initials: KG
authors.lastname: Harding
mesh_terms: D001943:Breast Neoplasms / Q000097:blood / Q000473:pathology / Q000601:surgery; D003300:Copper / Q000032:analysis* / Q000502:physiology; D005122:Exudates and Transudates / Q000737:chemistry*; D005260:Female; D006639:Histidine / Q000032:analysis / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008408:Mastectomy; D008991:Monitoring, Physiologic / Q000379:methods / Q000592:standards; D011199:Potentiometry / Q000379:methods* / Q000592:standards; D012016:Reference Values; D013997:Time Factors; D014364:Tryptophan / Q000032:analysis / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*; D015032:Zinc / Q000032:analysis* / Q000502:physiology
chemical_list: D006639:Histidine; D003300:Copper; D014364:Tryptophan; D015032:Zinc