Reliable method for the determination of ranitidine by liquid chromatography using a microvolume of plasma.
The aim of the present study was to develop a simple method to measure ranitidine, using 100 microL of plasma, by high-performance liquid chromatography with a Symmetry C(18) column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml(-1) and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml(-1), respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.
Flores Pérez
Carmen
C
Department of Pharmacology, Instituto Nacional de Pediatría, CP 04530 Mexico City, Mexico. carmenfloresp@hotmail.com
Juárez Olguín
Hugo
H
Flores Pérez
Janett
J
Toledo López
Alejandra
A
Lares Asseff
Ismael
I
Alvarez García
Carlos
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000097:blood*; D006801:Humans; D011899:Ranitidine / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D006635:Histamine H2 Antagonists; D011899:Ranitidine