In vitro model for the study of human posterior capsule opacification.
OBJECTIVE
To develop and evaluate a model for the organ culture of human lens capsules that reduces problems inherent in preexisting models for the study of in vitro posterior capsule opacification (PCO).


METHODS
Human lenses (N = 110) were isolated from donor eyes and supported externally within a lens holder system by medical-grade cyanoacrylate glue, allowing visualization of the entire capsular bag. After capsulorhexis and lens extraction were performed, the capsule specimens were maintained at physiological conditions for up to 4 weeks. The area of lens epithelial cell (LEC) coverage over the posterior capsule surface was determined objectively on a daily basis using a graticule. Lens epithelial cell behavior was correlated with clinical data and other in vitro PCO models.


RESULTS
Cyanoacrylate glue did not appear to be toxic to LECs at the concentration used. The amount of viable epithelium after nuclear extraction was dependent on the age and postmortem time of the specimen. Viable LEC cultures were obtained from eyes up to 9 days postmortem. The time from death to culture or from enucleation to culture did not influence LEC viability if it was fewer than 5 days. The LEC proliferation rates and confluence times were age dependent and correlated closely between pairs of eyes.


CONCLUSIONS
Results show that the lens holder model is a more physiological method for supporting the capsule and is a robust, reproducible system for the study of LEC migration and proliferation. It allows visualization within the entire capsular bag. Intraocular lenses can be implanted in this system in a way that more closely resembles the in vivo scenario. This model can be used to evaluate therapeutic measures to prevent PCO.
El-Osta
Austen A R
AA
Vision Research, the Rayne Institute, St Thomas' Hospital, London, United Kingdom.
Spalton
David J
DJ
Marshall
John
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019923:Capsulorhexis; D002386:Cataract / Q000473:pathology*; D002455:Cell Division; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D003487:Cyanoacrylates; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000473:pathology*; D008875:Middle Aged; D008954:Models, Biological; D009924:Organ Culture Techniques; D014019:Tissue Donors
D003487:Cyanoacrylates; C040229:MK 6 cyanoacrylate