c-Myc-deficient B lymphocytes are resistant to spontaneous and induced cell death.
C-myc gene is a member of the myc family of proto-oncogenes involved in proliferation, differentiation, and apoptosis. Overexpression of c-myc in fibroblasts causes apoptosis under low serum conditions in a process that requires the interaction of CD95 and CD95L on the surface. We have previously reported an in vivo conditional model to inactivate the c-myc gene in B lymphocytes. Here, we show that c-Myc-deficient primary B lymphocytes are resistant to different apoptotic stimuli. Nonactivated c-Myc-deficient B cells are resistant to spontaneous cell death. Upon activation, c-Myc-deficient B lymphocytes express normal surface levels of activation markers, and show resistance to staurosporine and CD95-induced cell death.
de Alborán
I Moreno
IM
Department of Immunology and Oncology (DIO) (Pharmacia-CSIC), Centro Nacional de Biotecnología (CNB-CSIC), Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain. imoreno@cnb.uam.es
Baena
E
E
Martinez-A
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D019013:CD40 Antigens / Q000276:immunology / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015847:Interleukin-4 / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D016271:Proto-Oncogene Proteins c-myc / Q000172:deficiency* / Q000235:genetics; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D016271:Proto-Oncogene Proteins c-myc; D019014:fas Receptor; D015847:Interleukin-4