title: Relationship among location of T-antigen-induced DNA distortion, auxiliary sequences, and DNA replication efficiency.
abstract: T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or two copies of the 21-bp repeat. In a construct with enhanced replication efficiency, bending occurred in a 69-bp cellular sequence located upstream of a single copy of the 21-bp repeat. Bending occurred both upstream of ori and in the three 21-bp repeats located downstream of ori in a construct with reduced replication efficiency. In a construct with no 21-bp repeats, DNA distortion occurred downstream of ori. The results indicate that SV40 DNA replication is enhanced when the structure of the regulatory region allows the DNA to form a bent structure upstream of the initial movement of the replication fork.
authors.affiliation: Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Okuley
authors.forename: Mindy
authors.initials: M
authors.lastname: Call
authors.forename: Tara
authors.initials: T
authors.lastname: Mitchell
authors.forename: Bugen
authors.initials: B
authors.lastname: Hu
authors.forename: Mary E
authors.initials: ME
authors.lastname: Woodworth
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011196:Potassium Permanganate / Q000494:pharmacology; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014779:Virus Replication
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D011196:Potassium Permanganate