Identification of telomerase activity in gametocytes of Plasmodium falciparum.
Telomerase, a specialized cellular reverse transcriptase, compensates the chromosome shortening during the replication of most eukaryotic cells and contributes to cellular immortalization in cell culture (in vitro) and cancerous cell (in vivo). In the present study, the telomerase activity in the gametocytes of Plasmodium falciparum was investigated. Here, we report for the first time, the presence of telomerase activity in the gametocytes of P. falciparum using P. falciparum telomere repeat amplification protocol (Pf-TRAP) assay and Southern blot hybridization. Telomerase inhibitors such as 7-deaza-dGTP and AZT-TP, when used with the cytoplasmic extract of gametocytes in the Pf-TRAP assay, efficiently inhibit the product, which confirms the presence of telomerase in the gametocytes. The presence of telomerase activity in the laboratory adapted local (eastern India) isolates of P. falciparum indicates that telomerase might be the major player in chromosomal end protection during replication. The finding suggests that telomerase can be a potent target for the transmission blocking vaccine and drugs for combating malaria caused by P. falciparum.
Raj
Dipak Kumar
DK
Institute of Life Sciences, Chandrasekharpur, Bhubaneswar 751 023, India.
Das
Bibhu Ranjan
BR
Dash
A P
AP
Supakar
Prakash C
PC
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D005854:Germ Cells / Q000187:drug effects / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere
D018894:Reverse Transcriptase Inhibitors; D019098:Telomerase