[Identification of four new ceramides from Anthopleura pacifica].
OBJECTIVE
To study the bioactive constituents from Anthopleura pacifica.


METHODS
Compounds were separated by Pyricularia oryzae bioassay-guided fractionation method with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence.


RESULTS
A portion showing activity against P. oryzae was obtained and from the portion four compounds were identified as N-hydroxyethyl-N-tetradecanoyl-(2S,3R)-octadecasphinga-4(E), 8(E)-dienine (a), N-hydroxyethyl-N-(9Z-hexadecenoyl)-(2S,3R)-octadecasphinga-4 (E), 8 (E)-dienine (b), N-hydroxyethyl-N-hexadecanoyl-(2S,3R)-octadecasphinga-4(E), 8 (E)-dienine (c) and N-hydroxyethyl-N-(13Z-docosenoyl-(2S,3R)-octadecasphinga-4(E), 8(E)-dienine(d).


CONCLUSIONS
All the four compounds are new ceramides.
Zhang
Shu-yu
SY
Resarch Center for Marine Drugs, College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Tang
Hai-feng
HF
Yi
Yang-hua
YH
Li
Ling
L
Zhou
Da-zheng
DZ
Zou
Zheng-rong
ZR
D000818:Animals; D001681:Biological Assay; D002518:Ceramides / Q000737:chemistry / Q000302:isolation & purification*; D003064:Cnidarian Venoms / Q000737:chemistry*; D003904:Mitosporic Fungi / Q000187:drug effects*; D008968:Molecular Conformation; D015394:Molecular Structure; D012615:Sea Anemones / Q000737:chemistry*
D002518:Ceramides; D003064:Cnidarian Venoms