Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici.
Mutagenesis of Erwinia rhapontici was performed to enhance the production of isomaltulose from sucrose. A mutant strain, BN 68089, was obtained through a screening process involving automated and miniaturized cultivation in Bioscreen C. This high-throughput, miniaturized screening system was optimized to identify the mutant strain, which had a conversion yield (90%) and productivity (194 g l(-1) h(-1)). The BN 68089 mutant cells were immobilized in sodium alginate and when operated in a packed bed reactor gave a yield of 89% and a productivity of 144 g l(-1) h(-1) of at 30 degrees C, the optimal temperature. Immobilized BN 68089 cells exhibited 8% and 15% higher yield and productivity, respectively, than those of the wild-type strain.
Ahn
Seung-Joon
SJ
BioNgene Co., Ltd. 10-1, 1Ka, Myungryun-Dong, Jongro-Ku, Seoul 110-521, Korea.
Yoo
Ji-Hyun
JH
Lee
Hyeon-Cheol
HC
Kim
Sang-Yong
SY
Noh
Bong-Soo
BS
Kim
Jung-Hoe
JH
Lee
Jung-Kul
JK
D019149:Bioreactors; D018914:Cells, Immobilized; D004885:Erwinia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D016296:Mutagenesis; D013395:Sucrose / Q000378:metabolism*; D013696:Temperature
D013395:Sucrose; D007534:Isomaltose; C008189:isomaltulose