Monoclonal antibody to parathyroid hormone-related protein induces differentiation and apoptosis of chondrosarcoma cells.
We investigated the effects of treatment with anti-parathyroid hormone-related protein (1-34) monoclonal murine antibody (anti-PTHrP MoAb) on apoptosis and the differentiation of chondrosarcoma HTB-94 cells. Treatment with anti-PTHrP MoAb accelerated apoptosis of HTB-94 cells in a dose-dependent manner, and anti-PTHrP MoAb also promoted the chondrogenic differentiation of HTB-94 cells. The induction of apoptosis by anti-PTHrP MoAb via imbalance of Bcl-2/Bax ratio and activation of caspase-3 may provide a mechanistic explanation for its potential antitumor effects. Our results suggest the possibility that anti-PTHrP MoAb may be beneficial as a new treatment for chondrosarcoma.
Miyaji
Takahiro
T
Department of Orthopaedic Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita 565-0871, Japan. miyaji@ort.med.osaka-u.ac.jp
Nakase
Takanobu
T
Onuma
Eturo
E
Sato
Koh
K
Myoui
Akira
A
Tomita
Tetsuya
T
Joyama
Susumu
S
Ariga
Kenta
K
Hashimoto
Jun
J
Ueda
Takafumi
T
Yoshikawa
Hideki
H
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002813:Chondrosarcoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D024121:Collagen Type X / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D044162:Parathyroid Hormone-Related Protein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
D000911:Antibodies, Monoclonal; C494826:BAX protein, human; D024043:Collagen Type II; D024121:Collagen Type X; D017931:DNA Primers; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases