title: Two separate replication modes of the bovine papillomavirus BPV1 origin of replication that have different sensitivity to p53.
abstract: We have shown previously that transient amplificational replication of reporter plasmids that carry the papillomavirus origin of replication is efficiently blocked by p53 protein in several cell lines. We demonstrate now that the replication of stably maintained episomal bovine papillomavirus BPV1 URR (upstream regulatory region) reporter plasmid is not sensitive to p53. In addition, these two replication modes--initial transient amplificational replication and stable maintenance replication of essentially the same BPV1 URR reporter plasmid--can take place in the same cells, where amplificational replication does not interfere with the stable maintenance replication. These data suggest that BPV1 replicons could follow two clearly separable replication mechanisms during initial amplification and during stable extrachromosomal maintenance.
authors.affiliation: Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, 23 Riia Street, 51010 Tartu, Estonia.
authors.forename: Ivar
authors.initials: I
authors.lastname: Ilves
authors.forename: Meelis
authors.initials: M
authors.lastname: Kadaja
authors.forename: Mart
authors.initials: M
authors.lastname: Ustav
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000187:drug effects* / Q000235:genetics; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004274:DNA, Recombinant; D004279:DNA, Viral; D016679:Genome, Viral; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects* / Q000502:physiology
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53