[Distribution of HIV-1 coreceptor CX3CR1 allelic polymorphisms in general population, HIV-1 high-risk group and HIV-1 carriers of Chinese indigenous Han and Uygur people].
OBJECTIVE
To investigate the V249I and T280M allelic polymorphisms of human immunodeficiency virus (HIV) coreceptor CX3CR1 in HIV-1 infected and uninfected population of Chinese indigenous Han and Uygur people and to probe the association between I249-M280 haplotype and HIV-1 susceptibility as well as AIDS progression.


METHODS
Genomic DNA of 223 Uygur subjects and 316 Han subjects were purified from PBMC. I249 and M280 allelic frequencies were identified by polymerase chain reaction (PCR)/nest polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. All data were tested by chi(2) or u statistics analysis.


RESULTS
Allelic frequencies of I249 and M280 were 16.1% and 13.3% in Uygur people, and 3.3% and 2.4% in Han people. No obvious difference existed between three groups of either ethnic group. However the allelic frequencies of HIV infected population were higher than those of general population, and those of general population higher than those of HIV-1 high-risk group. There was a strong linkage between I249 and M280 (P almost zero).


CONCLUSIONS
I249 mutation was the sine qua non of M280 mutation, and most I249 alleles were accompanied by M280. The frequency of I249-M280 haplotype in Uygur population (13.3%) was adjacent to Caucasian people (15.8%), and that of I249-T280 haplotype (2.8%) was obviously lower than Caucasian people (12.5%); while both of them in Han people were much lower (0.9% and 2.4%). I249-M280 haplotype could accelerate AIDS progression according to Faure et al, while might be associated with HIV-1 susceptibility.
Liu
Ming-xu
MX
Institute of Infectious Disease, People's Liberation Army 302 Hospital, Beijing 100039, China.
Wang
Fu-sheng
FS
Hong
Wei-guo
WG
Wang
Chang-qing
CQ
Wang
Bo
B
Jin
Lei
L
Hou
Jing
J
Lei
Zhou-yun
ZY
D000483:Alleles; D044466:Asian People / Q000235:genetics; D000073883:CX3C Chemokine Receptor 1; D002681:China / Q000453:epidemiology / Q000208:ethnology; D002893:Chromosomes, Human, Pair 3; D005006:Ethnicity; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D015728:Receptors, HIV / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D012307:Risk Factors
D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D008565:Membrane Proteins; D019707:Receptors, Chemokine; D015728:Receptors, HIV