Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum.
Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the alpha-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.
Green
Benedict T
BT
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 55108-6010, USA.
Lyte
Mark
M
Kulkarni-Narla
Anjali
A
Brown
David R
DR
D000818:Animals; D004553:Electric Conductivity; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D005260:Female; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation / Q000382:microbiology; D007583:Jejunum / Q000187:drug effects / Q000294:innervation* / Q000382:microbiology*; D008297:Male; D015213:Neuroimmunomodulation / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D010581:Peyer's Patches / Q000187:drug effects / Q000294:innervation* / Q000382:microbiology*; D019779:Salmonella enterica / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D012530:Saxitoxin / Q000494:pharmacology; D013552:Swine
D012530:Saxitoxin; D009638:Norepinephrine