The effect of different intraabdominal pressures on lipid peroxidation and protein oxidation status during laparoscopic cholecystectomy.
BACKGROUND
This prospective, randomized, and controlled study was designed to investigate the effects of different intraabdominal pressures (IAPs) on lipid peroxidation and protein oxidation status during laparoscopic cholecystectomy (LC).


METHODS
Twenty-four patients (12 men, 12 women) who underwent LC at either 10 or 15 mmHg of IAP were randomized into two groups. Repeated blood samples were collected to measure thiobarbituric acid reactive substances (TBARS) levels to assess lipid peroxidation and protein carbonyl content and protein sulfhydryl groups to assess protein oxidation status.


RESULTS
Serum protein carbonyls and TBARS levels were found to be increased immediately after desufflation in both study groups when compared to the preoperative levels. On the other hand, protein sulfhydryl levels were found to be decreased in both study groups. Although increases in protein carbonyls and TBARS levels were more prominent in patients who underwent LC at 15 mmHg of IAP, this difference was not statistically significant between both groups.


CONCLUSIONS
The results suggest that both 15 and 10 mmHg of LAP could lead to an increased oxidative stress response during LC, but no difference was found between the groups.
Polat
C
C
Department of General Surgery, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey. coskunpolat2001@yahoo.com
Yilmaz
S
S
Serteser
M
M
Koken
T
T
Kahraman
A
A
Dilek
O N
ON
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D002245:Carbon Dioxide / Q000008:administration & dosage / Q000494:pharmacology; D017081:Cholecystectomy, Laparoscopic; D005260:Female; D006801:Humans; D007327:Insufflation; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011312:Pressure; D013438:Sulfhydryl Compounds / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D001798:Blood Proteins; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002245:Carbon Dioxide