Identification of a gene rearranged by 2p21 aberrations in thyroid adenomas.
Thyroid adenomas belong to the cytogenetically best investigated human epithelial tumors. Cytogenetic studies of about 450 benign lesions allow one to distinguish between different cytogenetic subgroups. Two chromosomal regions, that is, 19q13 and 2p21, are frequently rearranged in these tumors. Although 2p21 aberrations only account for about 10% of the benign thyroid tumors with clonal cytogenetic deviations, 2p21 rearrangements belong to the most common cytogenetic rearrangements in epithelial tumors due to the high frequency of these benign lesions. The 2p21 breakpoint region recently has been delineated to a region of 450 kbp, but the gene affected by the cytogenetic rearrangements still has escaped detection. Positional cloning and 3' RACE-PCR allowed us to clone that gene which we will refer to as thyroid adenoma associated (THADA) gene. In cells from two thyroid adenomas characterized by translocations t(2;20;3) (p21;q11.2;p25) and t(2;7)(p21;p15), respectively, we performed 3'-RACE-PCRs and found two fusions of THADA with a sequence derived from chromosome band 3p25 or with a sequence derived from chromosome band 7p15. The THADA gene spans roughly 365 kbp and, based on preliminary results, encodes a death receptor-interacting protein.
Rippe
Volkhard
V
Center for Human Genetics, University of Bremen, Leobener Str. ZHG, D-28359 Bremen, Germany.
Drieschner
Norbert
N
Meiboom
Maren
M
Murua Escobar
Hugo
H
Bonk
Ulrich
U
Belge
Gazanfer
G
Bullerdiek
Jörn
J
D000236:Adenoma / Q000235:genetics*; D002869:Chromosome Aberrations; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D015321:Gene Rearrangement; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic
D009363:Neoplasm Proteins; C477698:THADA protein, human