title: Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene.
abstract: OBJECTIVE
  To identify esophageal cancer related gene2 (ECRG2) associated proteins and their possible interactions with ECRG2 gene.
  
  
  METHODS
  In the yeast forward two-hybrid system, ECRG2 was fused with the DNA-binding domain (DBD) of Gal4 and human fetal liver cDNA library was fused with the transcriptional activation domain (AD) of Gal4. We performed a high-stringency scale procedure to screen ECRG2 against human fetal liver cDNA library and characterized positives by sequence analysis.
  
  
  RESULTS
  We found the following 9 putatively associated proteins. They were metallothionein2A, metallothionein1H, metallothionein1G, ferritin, erythrocyte membrane protein band4.2, mitochondrial ribosomal protein S12, hypothetical protein FLJ10101, and a novel gene whose cDNA was found to have no strong homology to any other previously characterized gene whose DDBJ/EMBL/GenBank accession number is AF422192 mapped to human chromosome 14q31.
  
  
  CONCLUSIONS
  MT, a potential interaction partner for ECRG2, might be involved in the regulation of cell proliferation and apoptosis, and in various physiological processes. Determination of a reliability score for each single protein-protein interaction, especially interaction of ECRG2 and MT, permits the assignment of ECRG2 and unannotated proteins to biological pathways. A further understanding of the association between ECRG2 and MT should facilitate the functions of ECRG2 gene.
authors.affiliation: Department of Etiology and Carcinogenesis, Tumor Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Cui
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Zhang
authors.forename: Mei-Xia
authors.initials: MX
authors.lastname: Bi
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Guo
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Lu
mesh_terms: D005333:Fetus / Q000502:physiology; D015723:Gene Library; D006801:Humans; D008668:Metallothionein / Q000502:physiology*; D008969:Molecular Sequence Data; D053491:Proteinase Inhibitory Proteins, Secretory; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; C473799:SPINK7 protein, human; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins; D008668:Metallothionein