title: The absence of evidence for major effects of the frequent SNP +299G>A in the resistin gene on susceptibility to insulin resistance syndrome associated with Japanese type 2 diabetes.
abstract: Resistin, specifically secreted from adipocytes, antagonizes insulin and represents a promising candidate gene for type 2 diabetes. We reported that a frequent single nucleotide polymorphism (SNP) +299G>A in this gene is not associated with type 2 diabetes. To determine whether this SNP affects insulin resistance syndrome associated with type 2 diabetes, we examined its effects on susceptibility to obesity, hyperlipidemia and hypertension in type 2 diabetic subjects and on susceptibility to type 2 diabetes by interaction with other frequent genes involved in lipid metabolism, namely, beta3-adrenergic receptor (b3AR) Trp64Arg, phosphodiesterase 3B (PDE3B) c.1389G>A or lysosomal acid lipase (LAL) Thr-6Pro. The 99 type 2 diabetic and 99 control subjects were typed by PCR direct sequencing or PCR-RFLP. No differences in frequencies of obesity, hyperlipidemia and hypertension were found between the type 2 diabetic subjects with G/G and those with G/A or A/A genotypes of the resistin SNP. When the combination of the resistin SNP with each of b3AR, PDE3B and LAL SNPs was assessed, no association with type 2 diabetes was evident. Therefore, the frequent SNP +299G>A in the resistin gene is unlikely to have major effects on susceptibility to insulin resistance syndrome associated with type 2 diabetes in Japanese subjects.
authors.affiliation: Department of Laboratory Medicine, Ehime University School of Medicine, Shigenobu, 791-0295 Ehime, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ochi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Osawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onuma
authors.forename: Akiko
authors.initials: A
authors.lastname: Murakami
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishimiya
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimada
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kato
authors.forename: Ikki
authors.initials: I
authors.lastname: Shimizu
authors.forename: Koji
authors.initials: K
authors.lastname: Shishino
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Murase
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Fujii
authors.forename: Jun
authors.initials: J
authors.lastname: Ohashi
authors.forename: Hideichi
authors.initials: H
authors.lastname: Makino
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics; D000328:Adult; D000368:Aged; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D003920:Diabetes Mellitus / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics; D007333:Insulin Resistance / Q000235:genetics*; D007564:Japan; D008297:Male; D008875:Middle Aged; D009765:Obesity; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D052243:Resistin
chemical_list: D006729:Hormones, Ectopic; C499624:RETN protein, human; D052243:Resistin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517291:PDE3B protein, human