title: Peptide mapping and assessment of cryoprotective activity of 26/27-kDa dehydrin from soybean seeds.
abstract: To characterize the molecular weight diversity of seed dehydrin among soybean cultivars, 26/27-kDa soybean dehydrins were purified and compared in peptide mapping patterns, partial amino acid sequences, and cryoprotective activity on enzyme. In reverse phase chromatograms of their trypsin digests, we detected several distinctive peaks, one of which was attributed to a part of the internal glycine-rich region. Partial amino acid sequences of peptide fragments from trypsin and S. aureus V8 protease cleavage were found to be identical to the Mat9 translation. The CP50 of purified 26/27-kDa dehydrins were estimated to be 0.30 and 0.11 microM, respectively.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. michiko@affrc.go.jp
authors.forename: Michiko
authors.initials: M
authors.lastname: Momma
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kaneko
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Haraguchi
authors.forename: Ushio
authors.initials: U
authors.lastname: Matsukura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003451:Cryoprotective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010449:Peptide Mapping; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
chemical_list: D003451:Cryoprotective Agents; D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D010940:Plant Proteins; C070556:dehydrin proteins, plant; D007770:L-Lactate Dehydrogenase