Oropharyngeal carriage of Candida species in HIV-infected patients in India.
The present investigation represents the first study of oropharyngeal carriage of Candida and other yeasts in HIV-infected patients in India. One hundred and fifty HIV-positive patients were investigated by culturing their swish samples on plates of CHROMagar Candida. Ninety-eight patients (65.3%) were positive for Candida and four (2.7%) were positive for other yeasts. Among them, the first Indian C. dubliniensis isolate has been recovered. Molecular typing of selected C. albicans isolates by AP-PCR revealed two major genotypes based on the banding patterns. The susceptibilities of 30 Candida isolates to five antifungal agents including the new triazole voriconazole were determined in a micro-dilution test, according to the NCCLS protocol M 27. All the 22 C. albicans isolates were susceptible to five antimycotic agents (flucytosine, amphotericin B, fluconazole, voriconazole and itraconazole) except one isolate (VPCI-122), which was resistant to flucytosine (MIC > or = 64 mg l-1). The azole-resistant isolates reported here endorse the role of antifungal susceptibility testing whenever antifungal treatment with azoles is planned.
Gugnani
H C
HC
Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110 027, India. harishgugnani@hotmail.com
Becker
K
K
Fegeler
W
W
Basu
S
S
Chattopadhya
D
D
Baveja
U
U
Satyanarayana
S
S
Kalghatgi
T
T
Murlidhar
A
A
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology* / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000453:epidemiology* / Q000382:microbiology; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D015658:HIV Infections / Q000150:complications; D006801:Humans; D007194:India / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D009960:Oropharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000935:Antifungal Agents; D004271:DNA, Fungal