title: Mycelial incompatibility operative in pairings between single basidiospore isolates of Helicobasidium mompa.
abstract: Helicobasidium mompa is a clampless basidiomycete and binucleate both in secondary and primary hyphae. Single basidiospore isolates were paired in sibling and non-sibling combinations. We did not observe any alterations in colony morphology, such as tuft formation, but mycelial incompatibility, as indicated by the presence of a dark demarcation line between colonies, occurred in a unifactorial manner. Techniques using DNA molecular markers, such as internal transcribed spacer-restriction fragment-length polymorphism (ITS-RFLP), inter-simple-sequence-repeat polymerase chain reaction (PCR), and universally primed PCR, failed to identify nuclear migration to opposite single basidiospore isolates in all but one of the 92 pairings. These results imply that single basidiospore isolates of H. mompa may be incompetent to mate under laboratory conditions and that a single mycelial incompatibility factor operates in single basidiospore isolates.
authors.affiliation: National Institute for Agro-Environmental Sciences, 3-1-3 Kan-nondai, Tsukuba 305-8604, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Matsumoto
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D002467:Cell Nucleus / Q000502:physiology; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D018552:Daucus carota / Q000382:microbiology*; D005819:Genetic Markers; D025282:Mycelium / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers