Resistin messenger-RNA expression is increased by proinflammatory cytokines in vitro.
Resistin is a recently discovered polypeptide that induces insulin resistance in rodents. While in rodents resistin is predominantly expressed in adipocytes, in humans peripheral blood mononuclear cells (PBMC) seem to a be a major source of resistin. In the present study, we show that in human PBMC resistin mRNA expression-determined by fluorescence-based real-time polymerase chain reaction-is strongly increased by the proinflammatory cytokines interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-alpha), and also by lipopolysaccharides (LPS), respectively, while no effect was found by interferon-gamma (IFN-gamma) or leptin. Our results suggest that in humans resistin may be a link in the well-known association between inflammation and insulin resistance.
Kaser
S
S
Department of Medicine, University Hospital Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
Kaser
A
A
Sandhofer
A
A
Ebenbichler
C F
CF
Tilg
H
H
Patsch
J R
JR
D018925:Chemokines / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D006729:Hormones, Ectopic / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D052243:Resistin
D018925:Chemokines; D016207:Cytokines; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D012333:RNA, Messenger; D052243:Resistin