[Study on quantitative assay of chondroitin sulfate with a spectrophotometric method of azure A].
A simple, accurate and rapid spectrophotmeric method was proposed for the determination of chondroitin sulfate. The method was based on the absorbances of AA-CS complex at 625 nm being in proportion to the chondroitin sulfate concentrations. The linear range was 0-30 micrograms.mL-1 (R = 0.999), and the recovery range was 97.4%-103.8%. The quantities of chondroitin sulfate in different sample were determined in this way.
Gao
Gui-zhen
GZ
Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China.
Jiao
Qing-cai
QC
Ding
Yi-lei
YL
Chen
Lei
L
D001399:Azure Stains / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000032:analysis* / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D001399:Azure Stains; D002614:Chelating Agents; D004365:Drugs, Chinese Herbal; D002809:Chondroitin Sulfates; C025818:Azure A