The TATA-binding protein as a regulator of cellular transformation.
The TATA-binding protein, TBP, is used by all three RNA polymerases and is therefore central to the process of gene expression. TBP associates with several subsets of proteins, called TATA-binding protein-associated factors (TAFs). This results in the formation of at least three distinct complexes, SL1, TFIID, and TFIIIB, which dictates whether TBP functions in RNA polymerase (pol) I, pol II, or pol III transcription, respectively. The regulation of gene expression has focused largely on proteins that serve to modulate the efficiency by which the general transcription components, such as TBP, interact with promoters. The possibility of a basal transcription factor, itself, being regulated, and influencing cellular homeostasis, has not been extensively considered. However, recent studies have indicated that TBP is indeed regulated, and that modulation of its cellular concentration has a profound, and surprisingly selective, impact on gene expression that can mediate the normal proliferative responses of cells to growth stimuli as well as the transformation potential of cells.
Johnson
Sandra A S
SA
Department of Biochemistry and Molecular Biology, Keck School of Medicine and Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California 90033, USA.
Dubeau
Louis
L
White
Robert J
RJ
Johnson
Deborah L
DL
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D011905:Genes, ras / Q000235:genetics / Q000502:physiology; D006801:Humans; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D035361:Transcription Factor TFIIIB / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
C427472:BDP1 protein, human; C090956:BRF1 protein, S cerevisiae; C441028:BRF1 protein, human; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D035361:Transcription Factor TFIIIB; D016159:Tumor Suppressor Protein p53; D012321:DNA-Directed RNA Polymerases