title: Protein fouling in microfiltration: deposition mechanism as a function of pressure for different pH.
abstract: The influence of applied pressure on the fouling mechanism during bovine serum albumin (BSA) dead-end microfiltration (MF) has been investigated for a polyethersulfone acidic negatively charged membrane (ICE-450) from Pall Co. BSA solutions at pH values of 4, 5 (almost equal to the protein isoelectric point, IEP), and 6 were microfiltered through the membrane at different applied transmembrane pressures. Results have been analyzed in terms of the usual blocking filtration laws and a substantial change in the fouling mechanism was observed as the pressure was increased, this change can be related to the specific membrane-protein and protein-protein interactions.
authors.affiliation: Departamento de Termodinámica y Fi;sica Aplicada, Facultad de Ciencias Universidad de Valladolid, 47071 Valladolid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Velasco
authors.forename: M
authors.initials: M
authors.lastname: Ouammou
authors.forename: J I
authors.initials: JI
authors.lastname: Calvo
authors.forename: A
authors.initials: A
authors.lastname: Hernández
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D016062:Porosity; D011312:Pressure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine