title: Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.
abstract: A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.
authors.affiliation: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshida
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakano
authors.forename: Miki
authors.initials: M
authors.lastname: Kawada
authors.forename: Takahiko
authors.initials: T
authors.lastname: Oho
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Koga
mesh_terms: D018441:Biofilms; D003731:Dental Caries / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000302:isolation & purification*; D016986:Streptococcus sobrinus / Q000302:isolation & purification*; D019914:Taq Polymerase
chemical_list: D019914:Taq Polymerase