Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: validation in a multicenter collaborative trial.
As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C. coli, and C. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100%. The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories.
Lübeck
P S
PS
Danish Veterinary Institute, DK-1790 Copenhagen V, Denmark.
Cook
N
N
Wagner
M
M
Fach
P
P
Hoorfar
J
J
D020640:Arcobacter / Q000302:isolation & purification; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000145:classification / Q000302:isolation & purification; D002168:Campylobacter fetus / Q000145:classification / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000302:isolation & purification; D044863:Campylobacter lari / Q000145:classification / Q000302:isolation & purification; D005516:Food Microbiology / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011786:Quality Control; D015203:Reproducibility of Results; D019779:Salmonella enterica / Q000302:isolation & purification