Multiple HPV infection in cervical cancer screened by HPVDNAChip.
This study determined the distribution of high-risk HPV type infection in cervical cancer using newly developed oligonucleotide chips (HPVDNAChips). The study subjects included 80 cases of cervical neoplasia and 746 controls with a normal Pap smear. For HPV genotyping, the commercially available HPVDNAChips was used. The risk of cervical cancer was increased in women with a family history of cervical cancer (adjusted OR=2.3, 95% CI: 0.92-6.17) and in smokers (adjusted OR=3.2, 95% CI: 1.45-7.06). There was also a trend of increased risk with the number of full term pregnancies (P(for trend)<0.001). There were only 7.2% (54 of 746) infected high-risk HPV types in the control, whereas 54.5% (six of 11) and 76.5% (52 of 68) were infected in the CIN and cervical cancer, respectively. Multiple HPV infection was observed in 0.5% (three of 592) of the control group but in 9.1% (seven of 77) of cases. Multivariate analysis revealed that subjects infected with multiple HPV types had a 31.8-fold (95% CI: 7.50-134.75) higher risk of cervical cancer, while the single HPV type had a 19.9-fold increased risk (95% CI: 10.90-36.18) (P(for trend)<0.001). These results show that the detection and typing of HPV infection by HPVDNAChip can be a useful in clinical applications because it provides information on multiple infections and the types of HPV in addition to HPV infection status.
Lee
Sang Ah
SA
Department of Preventive Medicine, College of Medicine, Seoul National University, 28 Yongun-dong, Chongno-gu, Seoul 110-799, South Korea.
Kang
Daehee
D
Seo
Sang Soo
SS
Jeong
Jeongmi Kim
JK
Yoo
Keun Young
KY
Jeon
Yong Tark
YT
Kim
Jae Weon
JW
Park
Noh Hyun
NH
Kang
Soon Beom
SB
Lee
Hyo Pyo
HP
Song
Yong Sang
YS
D000328:Adult; D015992:Body Mass Index; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005190:Family; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D010298:Parity; D012016:Reference Values; D012907:Smoking; D011795:Surveys and Questionnaires; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears
D004279:DNA, Viral