title: Protein expressions in macrophage-derived foam cells: comparative analysis by two-dimensional gel electrophoresis.
abstract: OBJECTIVE
  To study the overall protein expression changes induced by oxidized low-density lipoprotein (ox-LDL) in the U937 foam cells.
  
  
  METHODS
  Foam cell model was established by incubating the human monoblastic leukemia (U937) cells with ox-LDL. Each protein samples in U937 control cells and U937 foam cells were separated by two-dimensional gel electrophoresis (2-DE). After the gels were stained by silver staining method, the images were analyzed by PDquest 2D-image-analysis software (Bio-Rad). Some of the spots were available via the Internet with links to the U937 proteomic map provided from the ExPASy Proteomics server.
  
  
  RESULTS
  Using 2-DE, the overall protein map was obtained, in which 150 spots were matched with the control gel (match ratio: 75 %). Compared with U937 cells, 37 spots significantly changed in the foam cells (P<0.05), among which the expression levels in 28 spots increased and those in 9 spots decreased. Especially, 8 spots in U937 cells were absent in the foam cells, while 11 spots in the foam cells were absent in the control cells.
  
  
  CONCLUSIONS
  The changed protein profiles induced by ox-LDL in U937 foam cells were established to support the functional studies on the macrophage-derived foam cells in atherosclerotic pathological states.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Yu
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Yang
authors.forename: Hui-Zhi
authors.initials: HZ
authors.lastname: Fan
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Huang
authors.forename: Yao-Cheng
authors.initials: YC
authors.lastname: Rui
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Yang
mesh_terms: D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005487:Foam Cells / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D020298:U937 Cells
chemical_list: D008077:Lipoproteins, LDL; D011506:Proteins; C099252:oxidized low density lipoprotein