Cyclospora cayetanensis: oocyst characteristics and excystation.
In Thailand in 1999-2000, Cyclospora oocysts from two HIV-infected patients and one patient with prolonged diarrhea were detected by formalin-ether concentration technique. Sporulation was performed by mixing stool samples in 2.5% potassium dichromate solution, sporulated oocysts were then treated with various solutions before mechanical rupturing in order to establish excystation, fewer than 10% of the sporulated oocysts could be excysted. Our techniques provided more details of the characteristic appearance of sporocysts and sporozoites within the oocysts (DMSO-modified acid-fast technique with our modification).
Siripanth
Chutatip
C
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmcsr@mahidol.ac.th
Phraevanich
Rangson
R
Suphadtanaphongs
Waraporn
W
Thima
Niramol
N
Radomyos
Prayong
P
D000818:Animals; D002460:Cell Line; D021744:Cyclospora / Q000254:growth & development*; D004285:Dogs; D015658:HIV Infections / Q000523:psychology; D006801:Humans; D013785:Thailand