Sugar arrays in microtiter plates.
Presented herein are a variety of methods for the attachment of saccharides to the surface of microtiter plates through long-chain hydrocarbons capable of binding to the polystyrene surface through noncovalent interaction. These glycolipids have the benefit of diverse and facile conjugation procedures and the ability to withstanding a range of biological assays. On addition of carbohydrates to the microtiter plate well, a suitable surface for both ELISA and enzymatic transformation is available, making the saccharide arrays useful for high-throughput study of specificity and inhibition in sugar-protein interactions.
Wong
Chi-Huey
CH
Department of Chemistry, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92307, USA.
Bryan
Marian C
MC
D000588:Amines / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D004987:Ethers / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry
D000588:Amines; D002241:Carbohydrates; D004987:Ethers; D014230:Triazoles