title: Photosynthetic genes are differentially transcribed during the dehydration-rehydration cycle in the resurrection plant, Xerophyta humilis.
abstract: One of the desiccation-tolerant mechanisms of the resurrection plant, Xerophyta humilis, is the ability to shut down photosynthesis reversibly. The X. humilis psbR and ChlP genes, encoding the 10 kDa polypeptide of photosystem II (PSII) and a geranylgeranyl reductase, respectively, were isolated in a differential display screen as dehydration-down-regulated and rehydration-up-regulated transcripts. Two other PSII genes, psbA (chloroplast-encoded) and psbP (nuclear-encoded), isolated by degenerate primer PCR, display a similar trend in expression.
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa. collett@science.uct.ac.za
authors.forename: Helen
authors.initials: H
authors.lastname: Collett
authors.forename: Rafal
authors.initials: R
authors.lastname: Butowt
authors.forename: James
authors.initials: J
authors.lastname: Smith
authors.forename: Jill
authors.initials: J
authors.lastname: Farrant
authors.forename: Nicola
authors.initials: N
authors.lastname: Illing
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D032181:Craterostigma / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers; D003890:Desiccation; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010788:Photosynthesis / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000235:genetics; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D045332:Photosystem II Protein Complex