title: Molecular cloning of canine RCAS1 cDNA.
abstract: Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen, strongly expressed in invasive cancers. RCAS1 inhibited the in vitro growth of immunocytes, and induced apoptotic cell death. The cloning of canine RCAS1 cDNA was carried out and identified from the mammary gland tumor of a dog. A canine RCAS1 cDNA of 864 bp in length has an open reading frame of 642 bp nucleotides encoding a protein of 213 deduced amino acids. The predicted amino acid sequence of canine RCAS1 showed 96.2% and 96.7% homologies with those of human and mouse RCAS1 respectively. Canine RCAS1 has an N-terminal transmembrane segment and a coiled-coil structure in the C-terminal protein, which are highly conserved in mouse and human RCAS1.
authors.affiliation: Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okamura
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ma
authors.forename: Tanvir S
authors.initials: TS
authors.lastname: Khatlani
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Une
authors.forename: Munekazu
authors.initials: M
authors.lastname: Nakaichi
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D005260:Female; D006801:Humans; D007107:Immune System / Q000502:physiology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000951:Antigens, Neoplasm; D017931:DNA Primers; D018076:DNA, Complementary