Characterization of CIM monoliths as enzyme reactors.
The immobilization of the enzymes citrate lyase, malate dehydrogenase, isocitrate dehydrogenase and lactate dehydrogenase to CIM monolithic supports was performed. The long-term stability, reproducibility, and linear response range of the immobilized enzyme reactors were investigated along with the determination of the kinetic behavior of the enzymes immobilized on the CIM monoliths. The Michaelis-Menten constant K(m) and the turnover number k(3) of the immobilized enzymes were found to be flow-unaffected. Furthermore, the K(m) values of the soluble and immobilized enzyme were found to be comparable. Both facts indicate the absence of a diffusional limitation in immobilized CIM enzyme reactors.
Vodopivec
Martina
M
National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
Podgornik
Ales
A
Berovic
Marin
M
Strancar
Ales
A
D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D015203:Reproducibility of Results
D004800:Enzymes, Immobilized