Study on interaction between T4 phage and Escherichia coli B by microcalorimetric method.
The process that T4 phages multiply in host cells of Escherichia coli B was determined using LKB-2277 Bioactivity Monitor by means of stopped-flow method, and the growth was measured turbidometrically at the same time at 37 degrees C. By analyzing thermo-curves, quantitative parameters could be obtained to characterize the interactions of host cells and phages. The parameters such as k(a), P(max), G etc. change regularly with the decrease of multiplicity of infection (MOI) value. Infection-lysis equations were fitted and the lytic rate constant k(L) was obtained. The results show that the metabolic activity of infected cells is more intensive than that of normal cells. The phenomenon of lysis inhibition (LIN) was first detected with the microcalorimetric method, and the mechanism is discussed.
Guosheng
Liu
L
College of Life Sciences, Wuhan University, Wuhan 430072, China. pingshen@whu.edu.cn
Yi
Liu
L
Xiangdong
Chen
C
Peng
Liu
L
Ping
Shen
S
Songsheng
Qu
Q
D001433:Bacteriolysis; D017122:Bacteriophage T4 / Q000254:growth & development / Q000502:physiology*; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D006358:Hot Temperature; D007700:Kinetics; D009391:Nephelometry and Turbidimetry / Q000379:methods