[Different effect of the ''recombinant'' bradykinin on the contractile activity of cultured atrial and ventricular cardiomyocytes].
The physiological activity of the "recombinant" bradykinin expressed by retrovirus recombinant pPS-3-neo (brd) was tested on cultural atrial (aCMC) and ventricular (vCMC) cardiomyocytes in newborn rats. The "recombinant" bradykinin was shown to have a chronotropic effect on aCMC and an inotropic effect on vCMC. The effects are in line with the action of the synthetic bradykinin preparation at a concentration of around 10(-15) M. A pretreatment of CMC by parmidine, i.e. a bradykinin antagonist, blocked the effect of bradykinin. The contractive CMC activity in the cultural cell medium, transferred by pPS-3-neo without the bradykinin gene, was not different from the control value.
Neverova
M E
ME
Research Center for Medical Genetics, Russia's Academy of Medical Sciences, Moscow, Russia.
Adamskaia
E I
EI
Terekhov
S M
SM
Ivanov
D S
DS
Prasolov
V S
VS
Kalinin
V N
VN
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002478:Cells, Cultured; D006325:Heart Atria / Q000166:cytology / Q000187:drug effects*; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009200:Myocardial Contraction / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D001920:Bradykinin
Razlichnoe vliianie "rekombinantnogo" bradikinina na sokrashchenie kul'tiviruemykh kardiomiotsitov predserdiĭ i zheludochkov.