Hepatoprotective constituents in plants. 14. Effects of soyasapogenol B, sophoradiol, and their glucuronides on the cytotoxicity of tert-butyl hydroperoxide to HepG2 cells.
The effects of soyasapogenol B, sophoradiol, their glucuronides, and glycyrrhizin on the hepatotoxicity of tert-butyl hydroperoxide (t-BuOOH) in a human-liver-derived cell line (HepG2 cells) were investigated. Glycyrrhizin showed significant dose-dependent protective effects against the cytotoxicity of t-BuOOH. Among soyasapogenol B and its glucuronides, the monoglucuronide showed the most potent hepatoprotective activity, followed by soyasapogenol B itself. Soyasaponin III was weakly protective, while soyasaponin I increased the toxicity of t-BuOOH. Among sophoradiol and its glucuronides, sophoradiol itself showed the most potent hepatoprotective activity, which was equal to glycyrrhizin, while the monoglucuronide and kaikasaponin III showed an increase in cytotoxicity. These results were considerably different from those reported previously on the protective effects of these compounds using primary cultures of immunologically injured rat liver cells. Consequently, the hepatoprotective action of the triterpene derivatives investigated would be different in HepG2 cells and in rat primary hepatocyte cultures.
Kinjo
Junei
J
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan. kinjojun@fukuoka-u.ac.jp
Hirakawa
Tomoki
T
Tsuchihashi
Ryota
R
Nagao
Tsuneatsu
T
Okawa
Masafumi
M
Nohara
Toshihiro
T
Okabe
Hikaru
H
D002240:Carbohydrate Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D020719:Glucuronides / Q000494:pharmacology; D019695:Glycyrrhizic Acid / Q000494:pharmacology; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*
D020719:Glucuronides; D012503:Saponins; C407096:soyasapogenol B; C417684:sophoradiol; D019695:Glycyrrhizic Acid; D009828:Oleanolic Acid; D020122:tert-Butylhydroperoxide