Towards on-line monitoring of cell growth in microporous scaffolds: Utilization and interpretation of complex permittivity measurements.
Here we demonstrate the ability to characterize microporous scaffolds and evaluate cell concentration variation via the utilization and interpretation of complex permittivity measurements (CP), a direct and nondestructive method. Polymer-based microporous scaffolds are of importance to tissue engineering, particularly in the promotion of cell adhesion, proliferation, and differentiation in predefined shapes. Chitosan gel scaffolds were seeded with increasing concentrations of macrophages to simulate cell growth. Complex permittivity measurements were performed using a dielectric probe and a vector network analyzer over a frequency ranging from 200 MHz to 2 GHz. An effective medium theory was applied to interpret the data obtained; respectively, Looyenga and Maxwell-Wagner-Hanai functions were used to retrieve the porosity and the variation of the cell concentration from the CP measurements. Calculated porosities were in agreement with experimental evaluation-porosity ranged from 81-96%. Changes in cell concentration inside the scaffolds upon injection of differing cell concentrations into the scaffold were detected distinguishably. Variations resulting from the cumulative injection of 400-1800 microL of 10(6) cells/mL solution into the scaffold were monitored. Results suggest that CP measurements in combination with an appropriate effective medium approximation can enable on-line monitoring of cell growth within scaffolds.
Bagnaninchi
Pierre-Olivier
PO
Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, Quebec H3A 2B4, Canada.
Dikeakos
Maria
M
Veres
Teodor
T
Tabrizian
Maryam
M
D000465:Algorithms; D000818:Animals; D002452:Cell Count / Q000379:methods*; D002455:Cell Division / Q000502:physiology*; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003198:Computer Simulation; D046508:Culture Techniques / Q000379:methods; D025802:Electric Capacitance; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D005109:Extracellular Matrix / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008567:Membranes, Artificial; D051379:Mice; D008954:Models, Biological; D009862:Online Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D023822:Tissue Engineering / Q000379:methods*
D008567:Membranes, Artificial; D002686:Chitin; D048271:Chitosan