Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea.
Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.
Chai
Jong-Yil
JY
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. cjy@plaza.snu.ac.kr
Lin
Aifen
A
Shin
Eun-Hee
EH
Oh
Myoung-Don
MD
Han
Eun-Taek
ET
Nan
Ho-Woo
HW
Lee
Soon-Hyung
SH
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D007723:Korea; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008875:Middle Aged; D018512:Parasitemia / Q000469:parasitology; D012510:Sarcoma 180; D012692:Serial Passage; D013047:Specific Pathogen-Free Organisms; D014122:Toxoplasma / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000469:parasitology; D014407:Tumor Cells, Cultured; D014774:Virulence
D000953:Antigens, Protozoan