title: Structure and expression of goat GLYCAM1 gene: lactogenic-dependent expression in ruminant mammary gland and interspecies conservation of the proximal promoter.
abstract: A macroarray approach used to list genes differentially expressed in goat mammary gland (gestation vs. lactation), other than milk protein genes, allowed us to detect the Glycosylation-dependent Cell Adhesion Molecule 1 (GLYCAM1) gene. GLYCAM1, a member of the glycoprotein mucin family, is a component of the milk fat globule membrane (MFGM). Its complete cDNA and gene sequences were determined and it was mapped by fluorescent in situ hybridization (FISH) on goat and cattle chromosome 5 (CHI5q21 and BTA5q21), and on sheep chromosome 3 (OAR3q21). Northern blot analyses confirmed its differential expression during the development and differentiation of the mammary gland of ruminants with a significantly higher mRNA amount during lactation than during pregnancy. An experimental in vivo induction model for lactation, developed by Kann et al., showed that the expression of GLYCAM1 is hormonally regulated in the mammary gland of ewes. Interspecies comparison of the gene promoter revealed the evolutionary conservation of a short proximal nucleotide sequence encompassing several transcription factor binding sites that could mediate the above-mentioned hormonal regulation.
authors.affiliation: Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France. leprovos@diamant.jouy.inra.fr
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Provost
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cassy
authors.forename: Hélène
authors.initials: H
authors.lastname: Hayes
authors.forename: Patrice
authors.initials: P
authors.lastname: Martin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019143:Evolution, Molecular; D005091:Exons; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005796:Genes / Q000235:genetics; D006041:Goats / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D010928:Placental Lactogen / Q000494:pharmacology; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D009077:Mucins; D014157:Transcription Factors; C075185:sulfated glycoprotein p50; D004247:DNA; D010928:Placental Lactogen