Recombinant Pseudomonas putida carrying both the dsz and hcu genes can desulfurize dibenzothiophene in n-tetradecane.
Pseudomonas putida IFO13696, a recombinant strain with dsz desulfurization genes, desulfurized dibenzothiophene (DBT) in water but not in n-tetradecane. By introducing into this recombinant strain the hcuABC genes that take part in the uptake of DBT in the oil phase into the cell, 82% of 1 mM DBT in n-tetradecane was degraded in 24 h by resting cells. The products of hcuABC genes thus acted in the uptake of DBT in n-tetradecane into the cells and were effective in desulfurization of DBT in the hydrocarbon phase.
Noda
Ken-ichi
K
Bio-Refining Process Laboratory Technical Cooperation Department, Japan Cooperation Center, Petroleum, 58F Sun-shine 60 3-1-1 Ikebukuro, Toshima-ku, Tokyo 170-6058, Japan. JZA00636@nifty.ne.jp
Watanabe
Kimiko
K
Maruhashi
Kenji
K
D000473:Alkanes; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D012240:Rhodococcus / Q000235:genetics*; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*; D014867:Water
D000473:Alkanes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D013876:Thiophenes; C024713:n-tetradecane; D014867:Water; D013455:Sulfur; C016366:dibenzothiophene