Enhancement of cytotoxic effect of bleomycin with transient permeabilization of plasma membrane by laser-induced multiple stress waves in vitro.
We investigated the effect of multiple stress waves with peak stress of less than 3 MPa on chemosensitivity of HeLa cells adhered on plastic. HeLa cells exposed to stress waves retained more than 95% of the viability found in untreated cells. The scanning electron microscopy of cells exposed to stress waves showed ruffling microvilli, indicating a change in the cell surface morphology. The cytotoxicity of bleomycin (BLM) on HeLa cells was enhanced by the stress waves exposure. Our findings demonstrated that the low-intensity stress wave would allow to deliver the BLM molecules into cytoplasm by repetition exposure.
Miyamoto
Yuuichi
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan. ymiyamoto@postman.riken.go.jp
Umebayashi
Yukihiro
Y
Koyano
Mikio
M
Wakita
Masayoshi
M
Nishisaka
Tsuyoshi
T
D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D013314:Stress, Mechanical
D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin