Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography.
The Chinese phytomedicinal formulation Sanqi Zongdai Pian, traditionally prepared from crude extracts from roots of Panax notoginseng (Araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (HSCCC). In each operation, 283 mg methanolic extract of five tablets was separated and yielded pure 157, 17, 13 and 56 mg of ginsenoside-Rb1, notoginsenoside-R1, ginsenoside-Re and ginsenoside-Rg1, respectively, n-hexane-n-butanol-water (3:4:7, v/v/v) was used for the two-phase solvent system of the HSCCC separation. The chemical structures of three ginsenosides and one notoginsenoside were elaborated by means of electrospray ionization MS-MS and NMR analysis.
Du
Qizhen
Q
Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. qizhendu@hotmail.com
Jerz
Gerold
G
Waibel
Reiner
R
Winterhalter
Peter
P
D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis*; D000097669:Dammaranes
D014315:Triterpenes; D000097669:Dammaranes