Changes of plasma membrane ATPase activities and proline accumulation in twigs of Casuarina under different growth conditions.
Twigs of Casuarina were collected from Dongshan and Xiamen Island, China. Crude plasma membrane preparation was obtained from Casuarina twigs by ultra-centrifugation. Sealed plasma membrane vesicles were isolated with aqueous PEG3350-Dextran T500 two phase partitioning. We studied the proline accumulation in twigs and the ATPase activities of plasma membrane of Casurina under different growth conditions. The results showed that free proline content and the PM ATPase activities from unfavorable growth conditions were obviously higher than those from favorable environment. This suggested that Casuarina responded to osmotic stress by increasing osmotic material content and the proton transportation activities of PM ATPase.
Liang
Jie
J
School of Life Sciences, Xiamen University, Xiamen 361005.
Yan
Chong Ling
CL
Li
Yu Hong
YH
Chen
Ying Hua
YH
Pan
Jie
J
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D019684:Magnoliopsida / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012965:Sodium Chloride / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D012987:Soil / Q000032:analysis; D014887:Weather
D012987:Soil; D012965:Sodium Chloride; D011392:Proline; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase