Development of markers for simple sequence repeat-rich regions that discriminate between Pisolithus albus and P. microcarpus.
Inter-simple sequence repeat PCR (ISSR-PCR) was used to develop markers for simple sequence repeat-rich (SSR) regions for investigation of genetic relatedness of Pisolithus isolates collected from eastern mainland Australia. Primers were designed to amplify ten SSR-rich regions and these were used to screen 14 Pisolithus isolates. Two amplified loci showed size polymorphisms among the isolates (regarded as polymorphic), two were monomorphic for all isolates, while the remainder amplified alleles for only some isolates. UPGMA analysis of the alleles for each isolate at each locus together with ITS-RFLP analysis, separated the isolates into groups. These two groups appear to correspond to isolates that ITS sequence data have previously separated as P. albus and P. microcarpus.
Hitchcock
Catherine J
CJ
Mycorrhiza Research Group, Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
Chambers
Susan M
SM
Anderson
Ian C
IC
Cairney
John W G
JW
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis; D005819:Genetic Markers; D018598:Minisatellite Repeats / Q000235:genetics*; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D005819:Genetic Markers