title: Experimental paracoccidioidomycosis in dogs.
abstract: The aim of this study was to evaluate the susceptibility of dogs to develop paracoccidioidomycosis by experimental infection. Puppies were inoculated with Paracoccidioides brasiliensis by an intravenous route and two out of four died 1 week postinoculation, showing, at histopathological analysis, granulomas in the lungs, spleen and liver. P. brasiliensis was isolated from these organs. The animals that survived the infection showed a strong reaction when skin was tested with gp43, a specific antigen of P. brasiliensis. These animals were killed at 1 and 5 months after infection, and no lesions, macroscopic or microscopic, were observed in the lungs, spleen or liver; furthermore no P. brasiliensis culture was obtained from these organs. These results suggest that dogs can develop paracoccidioidomycosis and reinforces the importance of this animal as a sensitive indicator of P. brasiliensis in the environment.
authors.affiliation: Universidade Estadual de Londrina, Departamento de Ciências Patológicas, Londrina, PR, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Ono
authors.forename: M O
authors.initials: MO
authors.lastname: Kishima
authors.forename: E N
authors.initials: EN
authors.lastname: Itano
authors.forename: A P F R L
authors.initials: AP
authors.lastname: Bracarense
authors.forename: Z P
authors.initials: ZP
authors.lastname: Camargo
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000946:Antigens, Fungal / Q000276:immunology; D004283:Dog Diseases / Q000382:microbiology* / Q000473:pathology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005656:Fungal Proteins / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D010228:Paracoccidioides / Q000302:isolation & purification / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013154:Spleen / Q000382:microbiology / Q000473:pathology
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000907:Antibodies, Bacterial; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins