Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay.
The INNO-LiPA Rif.TB assay is designed for the detection of rpoB gene mutations causing rifampin resistance in isolates. We applied the method directly to 60 Lithuanian and Danish clinical specimens to detect rifampin resistance rapidly. Results were obtained in 78.3% of clinical specimens, and all were concordant with those obtained by BACTEC 460. The assay could have major impact on the management of multidrug-resistant tuberculosis.
Johansen
Isik Somuncu
IS
International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Hvidovre University Hospital, Copenhagen, Denmark. isj@ssi.dk
Lundgren
Bettina
B
Sosnovskaja
Anaida
A
Thomsen
Vibeke Østergaard
VØ
D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D015994:Incidence; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000453:epidemiology
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin