title: Preventing DNA re-replication--divergent safeguards in yeast and metazoa.
abstract: Eukaryotes employ redundant mechanisms to limit the replication of genomic DNA to only once per cycle. These mechanisms prevent DNA re-replication by restricting the assembly of the pre-replication complex to the cell cycle stages of late mitosis and G1 phase so that the re-initiation of DNA replication cannot occur during S phase. Here we discuss the conserved yet divergent mechanisms of replication control employed in yeast and metazoan species, including a perspective on the newly uncovered role of the CUL-4 ubiquitin ligase as a central regulator of DNA replication in the nematode Caenorhabditis elegans.
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.forename: Edward T
authors.initials: ET
authors.lastname: Kipreos
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004330:Drosophila; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D064111:Minichromosome Maintenance Complex Component 2; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D051738:Origin Recognition Complex; D018741:Replication Origin / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D014981:Xenopus / Q000235:genetics / Q000378:metabolism
chemical_list: C066503:CDC6 protein, S cerevisiae; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; C475682:Cul-4 protein, C elegans; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051738:Origin Recognition Complex; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C499592:TAH11 protein, S cerevisiae; C406818:cdt1 protein, S pombe; D064111:Minichromosome Maintenance Complex Component 2; D008025:Ligases