title: [Haemophilus influenzae and betalactam resistance: description of bla TEM gene deletion].
abstract: The resistance to betalactam antibiotics in a total of 177 clinical isolates of Haemophilus influenzae, mostly from respiratory tract samples and characterized by their betalactamase production, was studied using the cephalosporin chromogenic assay and by detecting bla(TEM) and bla(ROB) genes. A substantial number of clinical isolates carrying the bla(TEM) gene, which presented a mutation consisting of the absence of a fragment of 136 base pairs, located upstream from the coding region including the 35 but not the 10 region of the promoter were found. This suggests that a new bla(TEM) promoter exists in these strains. This finding was associated with increased resistance to the antibiotics cefaclor and loracarbef compared with normal isolates. It was also found that 3.9% of the isolates carried the bla(ROB-1) gene.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Fe, Valencia, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Molina
authors.forename: J
authors.initials: J
authors.lastname: Córdoba
authors.forename: A
authors.initials: A
authors.lastname: Monsoliu
authors.forename: N
authors.initials: N
authors.lastname: Diosdado
authors.forename: M
authors.initials: M
authors.lastname: Gobernado
mesh_terms: D000328:Adult; D001483:Base Sequence; D002648:Child; D004269:DNA, Bacterial / Q000235:genetics; D017353:Gene Deletion; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000172:deficiency* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; C056342:beta-lactamase TEM-2; C477296:beta-lactamase bla(ROB-1), Haemophilus influenzae
vernacular_title: Haemophilus influenzae y resistencia a los betalactámicos: descripción de una supresión en el gen bla TEM.