title: [Suppression subtractive hybridization and its application in study of tumors].
abstract: Suppression subtractive hybridization (SSH) has been recently reported as a differential display technique at the basis of suppressive polymerase chain reaction(PCR). This technique has such advantages as easy and feasible, highly specific, capable of separating the low abundant specific fragments. Now it has been successfully applied in the study of tumors, developing biology, immune regulations, etc. Here we summarized its principle, method, and advances in the study of tumors, then analyzed its merits, defects, and applicable potentials.
authors.affiliation: Department of Pathology, The First Military Medical University, Guangzhou, Guangdong, 510515, PR China. redsnow007@sina.com
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Ding
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Shi
mesh_terms: D017209:Apoptosis; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015398:Signal Transduction