title: [Whole amount in situ hybridization and transgene via microinjection in zebrafish].
abstract: Zebrafish is new model organism to study development of the vertebrate. In this report, we chose a fragment of gene GATA-1 which specifically expressed in zebrafish hematopoiesis system as probe to carry on whole amount in situ hybridization. Then we transported reporter gene driven by GATA-1 promoter into the embryos via microinjection approach and observed the spatial and temporal expression pattern of GFP. Our results demonstrated that efficient and reliable technology of whole amount in situ hybridization and microinjection in zebrafish was established.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China.
authors.forename: Xue Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Ji Hua
authors.initials: JH
authors.lastname: Yao
authors.forename: Lu
authors.initials: L
authors.lastname: Cheng
authors.forename: Dong Wang
authors.initials: DW
authors.lastname: Wei
authors.forename: Da Ru
authors.initials: DR
authors.lastname: Lu
authors.forename: Jing Lun
authors.initials: JL
authors.lastname: Xue
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D017403:In Situ Hybridization; D008845:Microinjections; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D019076:Transgenes / Q000235:genetics; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins