Comparison of collagen fibre architecture between slow-twitch cranial and fast-twitch caudal parts of broiler M. latissimus dorsi.
1. Collagen fibre architectures of perimysium and endomysium in the slow-twitch cranial and fast-twitch caudal parts of broiler M. latissimus dorsi were compared. 2. Type I and III collagens were distributed in both perimysium and endomysium as indicated by their positive immunohistochemical reactions to polyclonal antibodies. 3. Cells invested by endomysium with no myofibres were larger in the cranial part because of the presence of larger slow-twitch myofibres. The honeycomb structure of endomysium was divided into several parts by thick perimysium. 4. The thick perimysial collagen fibres with parallel fibrils, which were interconnected by the loose reticular fibrils and thin fibres, were more numerous and thicker in the cranial part than the caudal. 5. Thick endomysial sidewall of cells in the cranial part was composed of a rougher reticulum of slightly thicker collagen fibrils compared with the thin sidewall in the caudal part. 6. These results indicated that both perimysial constitutions of collagen fibres and endomysial collagen fibrils had attained much larger growth in the slow-twitch cranial part than the fast-twitch caudal in broiler latissimus dorsi muscle.
Nakamura
Y N
YN
Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka, Japan.
Iwamoto
H
H
Tabata
S
S
Ono
Y
Y
D000818:Animals; D002645:Chickens / Q000033:anatomy & histology* / Q000502:physiology; D024042:Collagen Type I / Q000502:physiology / Q000648:ultrastructure*; D024061:Collagen Type III / Q000502:physiology / Q000648:ultrastructure*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D018656:Muscle Fibers, Fast-Twitch / Q000648:ultrastructure; D018657:Muscle Fibers, Slow-Twitch / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000502:physiology / Q000648:ultrastructure*
D024042:Collagen Type I; D024061:Collagen Type III