[Platelet phospholipase A2 mRNA content changes and cDNA cloning in rat blood with bacteria infection].
OBJECTIVE
To explore the changes of rat platelet phospholipase A2 (PLA2) mRNA content in bacteria infected rat and study the cDNA and amino acid sequences of the PLA2 structure to lay a good foundation for the development of new antibiotics.


METHODS
The PLA2 mRNA level in blood was determined by RT-PCR. The DNA sequence was cloned and analyzed.


RESULTS
After injection of bacteria in rats, the mRNA level of PLA2 in blood increased markedly. The cDNA and amino acid sequence were highly homologous to other PLA2 cDNA from different tissues of the rat.


CONCLUSIONS
Platelet PLA2 in blood responded quickly to bacteria infection in gene level. Therefore, the PLA2 protein was produced increasingly which was shown to control the infection with bacteria. Although there are little difference between PLA2 cDNA cloned from blood and other sources in DNA and amino acid sequences, the catalytic site for enzymatic activity and basic structure are identical.
Liu
Tao-tao
TT
Department of Pharmacology, Affiliated Tumor Hospital, Guangxi Medical University, Nanning 530021, China.
Liang
Ning-sheng
NS
Yang
Fan
F
Li
Yan
Y
Lu
Yi
Y
Meng
Zi-qing
ZQ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000097:blood*; D013211:Staphylococcus aureus
D018076:DNA, Complementary; D012333:RNA, Messenger; D010741:Phospholipases A; D054467:Phospholipases A2