title: Reciprocal Cdc25A and p27 expression in B-cell non-Hodgkin lymphomas.
abstract: Cell cycle regulation is often altered in cancer and deregulation of the cell cycle checkpoints is common in human neoplasia. The dual-specificity phosphatase Cdc25A and the cell cycle inhibitor p27 both play an important role in the regulation of the G1-S transition. We evaluated Cdc25A mRNA expression by in situ hybridization and p27 protein expression by immunohistochemistry in 42 histologically indolent B-cell non-Hodgkin lymphoma (NHL and 51 histologically aggressive B-cell NHL. Overexpression of Cdc25A (>50% tumor cells positive) was detected in 5 of 42 cases (12%) of histologically indolent B-cell NHL and in 29 of 51 (57%) of histologically aggressive B-cell NHL (P < 0.001). In contrast, high p27 protein expression (>50% tumor cells positive) was observed in 29 (69%) cases of indolent but in only one case (2%) of aggressive B-cell NHL (P < 0.0001). Thus, overexpression of Cdc25A and concomitant loss of p27 expression are associated with high grade B-cell NHL and may contribute to their aggressive biologic behavior.
authors.affiliation: Hematology and Transfusion Medicine Service, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Paulo, SP, Brazil.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Moreira Júnior
authors.forename: Gisele W B
authors.initials: GW
authors.lastname: Colleoni
authors.forename: M Giulia
authors.initials: MG
authors.lastname: Cangi
authors.forename: Michael
authors.initials: M
authors.lastname: Murphy
authors.forename: Bradford
authors.initials: B
authors.lastname: Sherburne
authors.forename: José O
authors.initials: JO
authors.lastname: Bordin
authors.forename: Massimo
authors.initials: M
authors.lastname: Loda
mesh_terms: D002452:Cell Count; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D009124:Muscle Proteins; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D012189:Retrospective Studies; D020687:cdc25 Phosphatases / Q000235:genetics* / Q000378:metabolism
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C058992:Tagln protein, mouse; C517004:CDC25A protein, human; C517005:Cdc25a protein, mouse; D020687:cdc25 Phosphatases