Planimetric and histological study of the aortae in atherosclerotic chickens treated with nifedipine, verapamil and diltiazem.
Calcium appears to be involved in many of the cellular events which are thought to be important in atherogenesis. Calcium channel blockers have been shown to reduce arterial lipid accumulation in animals without altering serum cholesterol. Avian models of atherosclerosis offer economic and technical advantages over mammalian models. In this study, we examine the effects of nifedipine, verapamil and diltiazem at clinical and higher doses, on the extent of atherosclerosis of egg-fed chickens. In order to assess the extent of atherosclerosis quantitatively, the aortic lesions of the thoracic and abdominal aorta, aortic arch and supraaortic regions were measured by planimetry. Atherosclerotic lesions were evaluated histologically. Statistically significant reductions in the lipid deposition of the aorta were found in all the treated groups. The extent and distribution of atherosclerotic lesions were decreased in a significant way by verapamil, nifedipine and diltiazem. The higher the dosage used, the higher the regression of the atherosclerotic lesions. At clinical dosage, nifedipine showed the highest decrease of the lesions. In addition, the chicken atherosclerosis model has proved itself useful and very suitable for in vivo drug intervention studies.
García Pérez
B
B
Clinical Universitary Hospital, Virgen de la Arrixaca, Murcia, Spain.
Ayala
I
I
Castells
M T
MT
Madrid
J F
JF
Ortega
M R
MR
Ortega
J V
JV
Ballesta
J
J
Fernández Pardo
J
J
Valdés
M
M
D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology* / Q000648:ultrastructure; D001013:Aorta, Thoracic / Q000473:pathology* / Q000648:ultrastructure; D001161:Arteriosclerosis / Q000188:drug therapy* / Q000473:pathology*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D002645:Chickens / Q000502:physiology*; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004396:Coloring Agents; D004036:Diet, Atherogenic; D004110:Diltiazem / Q000627:therapeutic use*; D008297:Male; D008854:Microscopy, Electron; D009543:Nifedipine / Q000627:therapeutic use*; D016707:Tissue Fixation; D014700:Verapamil / Q000627:therapeutic use*
D002121:Calcium Channel Blockers; D002791:Cholesterol, Dietary; D004396:Coloring Agents; D014700:Verapamil; D004110:Diltiazem; D009543:Nifedipine