title: The 'potyvirid primers' will probably provide phylogenetically informative DNA fragments from all species of Potyviridae.
abstract: A survey of gene sequences of species of Potyviridae, the most numerous family of plant viruses, has shown that the -GNNS- encoding region of the NIb gene is present in all c. 300 publicly available sequences of that region. We also report that relationships inferred from the sequence of the 1.6-2.1 kb portion of the genome between the -GNNS- encoding region and its 3' terminus reflect those of the remainder of the genome. Thus, the use for RT-PCR tests of the 'potyvirid primers' based on the -GNNS- encoding region and the 3' terminal poly-A region of the genome, will yield DNA fragments, the sequences of which are very likely to correctly identify viruses from which they were obtained.
authors.affiliation: School of Botany and Zoology, Faculty of Science, Australian National University, Canberra, ACT 0200, Australia. adrian.gibbs@anu.edu.au
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Gibbs
authors.forename: Anne M
authors.initials: AM
authors.lastname: Mackenzie
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Gibbs
mesh_terms: D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004279:DNA, Viral