The effects of DPPH on cochlear microcirculation.
To investigate the effects of the free radical, 1,1-Diphenyl-2-picylhydrazyl, on cochlear blood flow, 20 guinea pigs were divided into 3 groups at random, 6 for control group, 6 for 1 mmol/L group and 8 for 0.1 mmol/L group. 2 microliters vehicle or drugs were dropped into round window membrane (RWM). Cochlear microcirculation was monitored by laser Doppler flowmeter (LDF), and mean arterial blood flow (MABP), which was transferred by pressure conductor sensor and preamplifier, was simultaneously recorded on the computer. Our results showed that MABP was stable throughout the experiment. Cochlear blood flow (CBF) increased by 10.32% (P < 0.05) in 1 mmol/L group, and decreased by 4.89% in 0.1 mmol/L group (P < 0.05). In control group cochlear microcirculation showed no significant changes. It is concluded that DPPH exerted effects on cochlear microcirculation.
Kong
Weijia
W
Department of Otolaryngology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Chen
Min
M
D000818:Animals; D001713:Biphenyl Compounds; D001783:Blood Flow Velocity; D003051:Cochlea / Q000098:blood supply*; D005609:Free Radicals / Q000494:pharmacology; D006168:Guinea Pigs; D017078:Laser-Doppler Flowmetry; D008833:Microcirculation / Q000187:drug effects; D010851:Picrates / Q000494:pharmacology*; D011897:Random Allocation
D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl