Induction and temporal changes of osteopontin mRNA and protein in the brain following systemic lipopolysaccharide injection.
We analyzed expression of osteopontin (OPN), a cytokine regulating tissue repair and inflammation, in astrocytes and microglia in response to systemic lipopolysaccharide (LPS) administration (250 microg/100 g). OPN mRNA expression appeared in subpial astrocytes as early as 6 h, and then spread over the brain parenchyma. The signal for OPN mRNA reached a peak at 24 h post-injection, and returned to basal levels after 48 h. Changes in OPN immunoreactivity in the LPS-injected rat mirrored OPN mRNA induction patterns. These results provide the first evidence of OPN induction in astrocytes and microglia following peripheral immune challenge, and suggest that OPN may play a key role in the pathogenesis of neuroinflammation.
Choi
Jeong-Sun
JS
College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, 137-701 Seoul, South Korea.
Park
Hyun-Jung
HJ
Cha
Jung-Ho
JH
Chung
Jin-Woong
JW
Chun
Myung-Hoon
MH
Lee
Mun-Yong
MY
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000276:immunology*; D007031:Hypothalamus / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D008473:Median Eminence / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D053495:Osteopontin; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics* / Q000648:ultrastructure; D013495:Supraoptic Nucleus / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin