Correlation between protein expression and epigenetic and mutation changes of Wnt pathway-related genes in oral cancer.
The components of the Wnt-signaling pathway are reported to be mutated in human cancer cells, but the relationship between the components and oral squamous carcinoma (SCC) is still unknown. In this study, we analyzed the epigenetic changes and expression patterns of four member proteins of the Wnt-signaling pathway and analyzed the mutations of beta-catenin and AXIN 1 genes, in order to explore the roles of the pathway in the development of oral cancer. The results showed that there are no beta-catenin and AXIN 1 gene mutations and no methylation of the CpG island of beta-catenin, AXIN I and GSK3beta genes in oral cancer cells; methylation of the CpG island of APC occurs in the precancerous stage and it is a dynamic change; the aberrant expressions or abnormal localization of the Wnt-signaling pathway proteins have no relationship with methylation status or mutation. From our results, we suggest that the Wnt pathway related genes play a very limited role in the development of oral SCC.
Yeh
Kun-Tu
KT
Department of Molecular Medicine, China Medical College Hospital, Taichung, Taiwan, R.O.C.
Chang
Jan-Gowth
JG
Lin
Ting-Hsu
TH
Wang
Yu-Fen
YF
Chang
Jan-Yi
JY
Shih
Mu-Chin
MC
Lin
Cheng-Chieh
CC
D000328:Adult; D000368:Aged; D060466:Axin Protein; D003062:Codon; D018899:CpG Islands; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin
C555451:AXIN1 protein, human; D060466:Axin Protein; C495270:CTNNB1 protein, human; D003062:Codon; D003598:Cytoskeletal Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin