[Analysis on quality control of blood in blood banks in a five-year program in Hunan province].
OBJECTIVE
To investigate the blood quality of blood banks.


METHODS
HBsAg, Anti-HCV, Anti-HIV were detected by enzyme linked immunoadsorbent assay (ELISA). Treponema pallidum was detected by Trust or rapid plasma regain card test (RPR) before August 2001 and ELISA was used afterwards. Alanine aminotransferase (ALT) was detected by Reitman-Frankel before December 1999 and by continuous monitoring assay afterwards. Results were compared between (1) sampling and spot check, (2) different determent methods, (3) two kinds of reagents with different antigen coating.


RESULTS
Seventy-two (8.28 per thousand ) of the 8,699 plasma samples were found unqualified with levels of anti-HCV, ALT, HBsAg, Treponema pallidum and anti-HIV 24 (2.76 per thousand ), 19 (2.18 per thousand ), 16 (1.84 per thousand ), 7 (0.80 per thousand ), 6 (0.69 per thousand ), respectively. There were significant differences between different determent methods and different reagents, but only ALT showed significant difference in the sampling and spot check.


CONCLUSIONS
The unqualified samples were associated with testing methods, quality of reagents as well as ability and responsibility of the staff.
Lu
Yue-Feng
YF
Hunan Center for Clinical Laboratory, Changsha 410008, China.
Yin
Peng
P
Liu
Tao-Ying
TY
D000410:Alanine Transaminase / Q000097:blood; D001771:Blood Banks / Q000592:standards*; D001782:Blood Donors; D017848:Blood-Borne Pathogens / Q000302:isolation & purification; D002681:China; D051263:D-Alanine Transaminase; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood; D006679:HIV Seropositivity; D006514:Hepatitis B Surface Antigens / Q000097:blood; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D011786:Quality Control
D015483:HIV Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D000410:Alanine Transaminase; D051263:D-Alanine Transaminase