[The morphological and immunological characteristics of less-differentiated acute myeloid leukemic cells].
The aim was to study the morphological, histochemical and immunological characteristics of less-differentiated acute myeloid leukemic cells, and their diagnostic significance. Wright-Giemsa and histochemical staining were used to stain bone marrow smears from 2 case of AML-Mo. Immunological phenotypes were determined with flow cytometry. The results showed that myeloperoxidase stainings of both cases were negative, PAS was positive with fine particles, CD33/CD13 were positive, CD2/CD3/CD10/CD19/CD22 were negative. It is concluded that morphology, histochemistry and immunological phenotype on bone marrow smears are the main diagnostic basis for AML-Mo. The use of multiple monoclonal antibodies for staining may improve the accuracy.
Lai
Yi-Yan
YY
Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China. cq2@21cn.com
Tan
Huo
H
Ye
Xu
X
Zhou
Xu-Hong
XH
Feng
Ying
Y
Zeng
Lin-Juan
LJ
D002454:Cell Differentiation; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged