Protective effects of a plant histaminase in myocardial ischaemia and reperfusion injury in vivo.
Grass pea seedling histaminase (a copper-diamine oxidase) was found to exert a significant cardioprotection against post-ischaemic reperfusion damage. Electrocardiogram (ECG) recordings from the rats subjected in vivo to ischaemia and reperfusion showed ventricular tachycardia (VT) and ventricular fibrillations (VF) occurring in 9 out of 12 untreated rats whereas no ventricular arrhythmias were found under histaminase (80U/kg body weight) treatment (n=16 rats). Computer-assisted morphometry of the ischaemic reperfused hearts stained with nitroblue tetrazolium showed the extension of damaged myocardium (area at risk and infarct size) significantly reduced in rats treated with histaminase, in comparison with the non-treated rats, whereas no protection was found with the semicarbazide inactivated histaminase. Biochemical markers of ischaemia-reperfusion myocardial tissue damage: malonyldialdehyde (MDA), tissue calcium concentration, myeloperoxidase (MPO), and apoptosis indicator caspase-3 were significantly elevated in untreated post-ischaemic reperfused rats, but significantly reduced under histaminase protection. In conclusion, plant histaminase appears to protect hearts from ischaemia-reperfusion injury by more than one mechanism, essentially involving histamine oxidation, and possibly as reactive oxygen species scavenger, presenting good perspectives for a novel therapeutic approach in treatment of ischaemic heart pathology.
Masini
Emanuela
E
Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Pierpaoli
Simone
S
Marzocca
Cosimo
C
Mannaioni
Pier Francesco
PF
Pietrangeli
Paola
P
Mateescu
Mircea Alexandru
MA
Zelli
Massimo
M
Federico
Rodolfo
R
Mondovì
Bruno
B
D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy*; D015428:Myocardial Reperfusion Injury / Q000175:diagnosis* / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D018532:Pisum sativum / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000378:metabolism; D016896:Treatment Outcome
D002316:Cardiotonic Agents; D006631:Amine Oxidase (Copper-Containing)