[Effects of BIO-1211 on eosinophil chemotaxis, recruitment and mediator release].
To study the effects of very late antigen(VLA) antagonist BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.



Eosinophil chemotaxis was induced by platelet activating factor(PAF) in vitro and eosinophil recruitment and release were determined in vivo.



VLA antagonist BIO-1211 inhibited eosinophil chemotaxis induced by PAF. The inhibitory rates at 4x10(-11), 4x10(-10), 4x10(-9) mol x L(-1) were 24.9%, 29.9%, and 31.3%, respectively. Pretreatment by BIO-1211 1, 3 and 10 mg x kg(-1) intraperitoneally inhibited the recruitment of eosinophils in PAF in the rat induced by Sephadex in a dose dependent manner. Inhibitory rates were 60.3%, 68.9%, and 72.9%(P<0.05), respectively. BIO-1211 did not inhibit eosinophil peroxidase(EPO) release from eosinophils.



BIO-1211 inhibits eosinophil chemotaxis and recruitment, alleviates local inflammation, and may represent a new type of drug for allergic diseases.
Zhao
Xiao-yan
XY
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Ji-qiang
JQ
Xie
Qiang-min
QM
Tang
Hui-fang
HF
Bian
Ru-lain
RL
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000187:drug effects* / Q000502:physiology; D039041:Integrin alpha4beta1 / Q000037:antagonists & inhibitors* / Q000502:physiology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D039041:Integrin alpha4beta1; D009842:Oligopeptides; D010972:Platelet Activating Factor; C118154:BIO 1211; D047209:Eosinophil Peroxidase; D010544:Peroxidases