title: Clinical relevance of matrix metalloproteinase-13 determined with a new highly specific and sensitive ELISA in ascitic fluid of advanced ovarian carcinoma patients.
abstract: Matrix metalloproteinases (MMPs) are involved in many physiological and pathophysiological processes, including tumor cell invasion and metastasis. For one member of this family, MMP-13 (collagenase-3), a new, highly specific ELISA with a sensitivity of 0.5 ng MMP-13/ml was established. The protein levels of MMP-13 in ascitic fluids of 30 patients with advanced ovarian cancer FIGO stage III (n = 19) and IV (n = 11) were measured with this ELISA. Using a cut-off value of 0.5 ng MMP-13/mg total protein, two patient subpopulations with short (median 16 months) and long (median 36 months) overall survival were identified. Together with other prognostic markers, determination of MMP-13 in ascitic fluid may help to identify patients at risk for early death and help to individualize adjuvant therapy.
authors.affiliation: Department of Chemistry, Biochemistry I, University of Bielefeld, D-33615 Bielefeld, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hantke
authors.forename: Nadia
authors.initials: N
authors.lastname: Harbeck
authors.forename: Barbara
authors.initials: B
authors.lastname: Schmalfeldt
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Claes
authors.forename: Oliver
authors.initials: O
authors.lastname: Hiller
authors.forename: Marc-Oliver
authors.initials: MO
authors.lastname: Luther
authors.forename: Anita
authors.initials: A
authors.lastname: Welk
authors.forename: Walther
authors.initials: W
authors.lastname: Kuhn
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmitt
authors.forename: Harald
authors.initials: H
authors.lastname: Tschesche
authors.forename: Bernd
authors.initials: B
authors.lastname: Muehlenweg
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001202:Ascitic Fluid / Q000201:enzymology* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D017364:Collagenases / Q000032:analysis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D053509:Matrix Metalloproteinase 13; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology; D012680:Sensitivity and Specificity; D015996:Survival Rate
chemical_list: D000911:Antibodies, Monoclonal; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse