Identification of mature plasma cells in early rat yolk sac. A possible origin from the endodermal cell layer: immunohistochemistry and immunoelectron microscopic study.
Plasma cells play a pivotal role in the immune system and are responsible for the synthesis and release of immunoglobulins. Numerous in vitro culture experiments on the yolk sac demonstrated the generation of mature cells of the myeloid and lymphoid lineages under appropriate culture conditions. However, there are no reports describing the development of mature lymphoid cells in the yolk sac so far. For this reason, we undertook this study to investigate the development of antibody-containing plasma cells during early yolk sac haematopoiesis. Immunohistochemistry and immunoelectron microscopy were employed in the study. Results of this work demonstrated very weak immune staining for the intracytoplasmic IgA, IgG, and IgM at days 10 and 11 of embryonic life, while dark staining was obtained at 12 days. Positive staining was localized to the endodermal cell layer. Electron microscopic examinations revealed the existence of cells with the typical characteristics of plasma cells inside the endodermal cell layer, which may suggest their endodermal origin. To further verify the nature of these cells, intracytoplasmic immunoglobulins were demonstrated by immunoelectron microscopy. The present study demonstrated emergence of mature functioning plasma cells in early rat yolk sac. In a previous work we hypothesized the possibility of endodermal origin of yolk sac macrophages. This study adds additional evidence to support that hypothesis. The possible role of plasma cells in the yolk sac is discussed.
El-Nefiawy
Nagwa
N
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Handayama, Hamamatsu, Japan. nagwaebrahim@hotmail.com
Abdel-Hakim
Khaled
K
Kanayama
Naohiro
N
Suganuma
Nobuhiko
N
Terao
Toshihiko
T
D000818:Animals; D004707:Endoderm / Q000648:ultrastructure; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D010950:Plasma Cells / Q000276:immunology* / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D015017:Yolk Sac / Q000276:immunology* / Q000648:ultrastructure
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M