P53 gene of chang-liver cells (Atcc-Ccl13) exposed to aflatoxin B1 (Afb): the effect of lysine on mutation at codon 249 of exon 7.
The effect of different regimes of lysine-pre treatment on mutation at the 3rd nucleotide base of codon 249 which is located at the 7th exon of p53 gene of Chang-liver cells (CCIL13) exposed to aflatoxin B1 (AFB1) has been investigated. There is an indication of inhibition of 1 ug/ml AFB1--induced mutation by pretreatment of cells for 72 hr with 5-molar fold lysine equivalent of 1 ug/ml AFB1 1 u/g ml AFB1 was the does at which there was 50% survival among the cells of CC13 in cytotoxicity studies. The results suggest chemo prevention of AFB1 induced mutation at codon 249 locus of Exon 7 in CCL13's p53 gene and by implication, maybe, AFB1-induced primary liver cancer.
Uwaifo
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Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.
D016604:Aflatoxin B1 / Q000009:adverse effects* / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000517:prevention & control*; D003043:Cocarcinogenesis; D003062:Codon / Q000235:genetics*; D003601:Cytotoxicity Tests, Immunologic; D004279:DNA, Viral / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure / Q000009:adverse effects*; D005091:Exons / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000517:prevention & control*; D008239:Lysine / Q000737:chemistry / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D003062:Codon; D004279:DNA, Viral; D016604:Aflatoxin B1; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases; D008239:Lysine