Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma.
Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanisms by which these cells migrate into the airways are not fully understood. The present study investigated the presence of the eosinophil-activating chemokines regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemotactic proteins (MCP)-3 and -4, and eotaxins-1 and -2 in the bronchoalveolar lavage (BAL) fluid obtained from both asthmatic (n=10, age 6-10 yrs) and normal children (n=10, age 5-10 yrs). Measurements of chemokines in BAL fluid showed that levels of RANTES, MCPs-3 and -4, and eotaxins-1 and -2 were significantly increased in fluid obtained from asthmatic children when compared with normal children. Among the different chemokines, RANTES was the cytokine released in greatest quantities in BAL fluid from asthmatic children. There was a significant correlation between the concentrations of MCP-4 and eosinophil numbers in BAL fluid and a trend between both chemokines MCP-3 and eotaxin-2 and eosinophils. Interestingly, the levels of most chemokines correlated with one another. These findings suggest that RANTES monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 may regulate eosinophil trafficking into the airways of asthmatic children in a coordinated manner.
Rojas-Ramos
E
E
Dept of Allergy and Clinical Immunology, National Institute of Lung Diseases, Mexico DF, Mexico.
Avalos
A F
AF
Pérez-Fernandez
L
L
Cuevas-Schacht
F
F
Valencia-Maqueda
E
E
Terán
L M
LM
D001249:Asthma / Q000378:metabolism* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5 / Q000378:metabolism*; D054410:Chemokine CCL7; D019742:Chemokines, CC / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines; D004804:Eosinophils / Q000502:physiology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D018945:Monocyte Chemoattractant Proteins / Q000378:metabolism*
C515456:CCL11 protein, human; C099677:CCL13 protein, human; C515484:CCL24 protein, human; C515450:CCL7 protein, human; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D019742:Chemokines, CC; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins