title: Inhibitory effects of DA-9601 on ethanol-induced gastrohemorrhagic lesions and gastric xanthine oxidase activity in rats.
abstract: The exposure of gastric mucosa to ethanol produces pathological changes such as inflammatory process, hemorrhagic erosions, even acute ulcers. The gastric mucosal lesions accompanied by a significant decrease of gastric blood flow and increase of reactive oxygen species (ROS) implicate a role of xanthine oxidase in ethanol-induced gastric hemorrhagic erosions. DA-9601, a novel antipeptic formulation of extracts of Artemisia asiatica Nakai, was studied for its inhibitory effect on gastric xanthine oxidase activity and type conversion of the enzyme that has a profound role in free radical generation. Intubation of absolute ethanol (4 g/kg) significantly induced gastrohemorrhagic lesions and lipid peroxidation in the rat stomach. Oral administration of DA-9601 at 40 mg/kg body weight significantly reduced ethanol-induced gastric mucosal hemorrhagic lesions and lipid peroxidation, which was proportional to the inhibitory effect of DA-9601 on alcohol-induced xanthine oxidase-type conversion and enzyme activity. The results suggest that alcohol-induced gastric mucosal damage may be, in part, due to the increased activity of xanthine oxidase and type conversion rate of the enzyme and that the preventive effect of DA-9601 on gastrohemorrhagic lesions would result from its inhibitory action against xanthine oxidase and oxidative stress in alcohol-treated rats.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan 712-749, South Korea.
authors.forename: Keun
authors.initials: K
authors.lastname: Huh
authors.forename: Tae Hyup
authors.initials: TH
authors.lastname: Kwon
authors.forename: Uk Sup
authors.initials: US
authors.lastname: Shin
authors.forename: Won Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Byoung Ok
authors.initials: BO
authors.lastname: Ahn
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Oh
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D019306:Artemisia / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000627:therapeutic use*; D000431:Ethanol / Q000633:toxicity*; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D000897:Anti-Ulcer Agents; C116959:DA 9601; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000431:Ethanol; D014969:Xanthine Oxidase