A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction.
OBJECTIVE
To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.


METHODS
Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.


METHODS
Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.


METHODS
Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).


RESULTS
No statistical differences were observed in any of the parameters investigated.


CONCLUSIONS
SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).
Laursen
Steen B
SB
Fertility Clinic, Odense University Hospital, Copenhagen, Denmark. slaursen@health.sdu.dk
Andersen
Claus Yding
CY
Hindkjaer
Johnny
J
Erb
Karin
K
D000328:Adult; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods* / Q000592:standards*; D003718:Denmark; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011247:Pregnancy; D012099:Reproductive Techniques; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa