title: Standardisation and evaluation of DNA-lanthanide fluorescence spectroscopy for determining rifampicin resistance in Mycobacterium tuberculosis clinical isolates.
abstract: METHODS
  Tuberculosis Research Centre, Chennai.
  
  
  OBJECTIVE
  To rapidly identify multidrug-resistant Mycobacterium tuberculosis using a novel method.
  
  
  METHODS
  A new assay, based on DNA-lanthanide fluorescence, was standardised and evaluated using 93 each of coded rifampicin-resistant and rifampicin-sensitive M. tuberculosis clinical isolates for the correct identification of rifampicin resistance. The results obtained by the new assay were compared with the conventional results.
  
  
  CONCLUSIONS
  The new assay gave a sensitivity and specificity of 88% and 85%, respectively. It is simple, easy to perform and requires 48 hours for the drug susceptibility results to be available after obtaining the primary culture.
authors.affiliation: Tuberculosis Research Centre (Indian Council of Medical Research), Chetpet, Chennai, India.
authors.forename: C
authors.initials: C
authors.lastname: Mani
authors.forename: N
authors.initials: N
authors.lastname: Selvakumar
authors.forename: N
authors.initials: N
authors.lastname: Gajendiran
authors.forename: B
authors.initials: B
authors.lastname: Panigrahi
authors.forename: P
authors.initials: P
authors.lastname: Venkatesan
authors.forename: P R
authors.initials: PR
authors.lastname: Narayanan
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial; D006801:Humans; D028581:Lanthanoid Series Elements; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity*; D012016:Reference Values; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D028581:Lanthanoid Series Elements; D012293:Rifampin