Multidrug resistance proteins and topoisomerase IIalpha expression in colon cancer: association with metastatic potential.
OBJECTIVE
To investigate the role of multidrug resistance proteins and topoisomerase IIalpha in colon cancer.


METHODS
Tissue sections from 89 Dukes' stage B-D colon cancer patients were selected. The expression of multidrug resistance proteins and topoisomerase IIalpha in primary tumour cells was assessed by standard immunohistochemistry. The extent of their expression was measured by image analysis and was correlated with clinicopathological features of the patients.


RESULTS
P-glycoprotein was associated with the presence of lymph node metastasis (P=0.005), vessel invasion (P=0.0001) and perineural invasion (P=0.020).


CONCLUSIONS
P-glycoprotein is probably involved in the processes of local invasion and metastatic dissemination.
Zorzos
Haralampos S
HS
Department of Pathology, School of Medicine, National and Capodistrian University of Athens, Greece. alazaris@med.uoa.gr
Lazaris
Andreas C
AC
Korkolopoulou
Penelope A
PA
Kavantzas
Nikolaos G
NG
Tseleni-Balafouta
Sofia
S
Patsouris
Efstratios S
ES
Tsavaris
Nikolaos V
NV
Davaris
Panagiotis S
PS
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D000230:Adenocarcinoma / Q000378:metabolism* / Q000556:secondary; D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins; D018432:Drug Resistance, Multiple / Q000502:physiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness
D018435:ATP Binding Cassette Transporter, Subfamily B; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins