title: [Researching a novel NPC-related candidate suppressor gene BRD7 by two-dimensional gel electrophoresis and MALDI-TOF-MS].
abstract: BRD7 was isolated through cDNA representational difference analysis (RDA) (GenBank accession No. AF152604). Previous studies showed that BRD7 gene was down-regulated in nasopharyngeal carcinoma (NPC) cells and tissues, and three cSNPs (coding-region single nucleotide polymorphisms) were found on BRD7. In addition, six BRD7-interacting proteins were identified by yeast two-hybrid system. To study the function of this gene on carcinogenesis in NPC, BRD7 gene was transfected into HNE1 cells low-expressed BRD7 by using liposomes and a stable cell line over-expressing BRD7 was established. After two-dimensional gel electrophoresis(2-DE), twenty differentially expressed proteins were identified by MALDI-TOF-MS including argininosuccinate lyase, metalloproteinase inhibitor-2 precursor, proteaseome activator28 beta subunit, thyroid transcriptional factor-1, cyclinH (MO15-associated protein), and so on. These differentially expressed proteins are related to cell cycling, transcription regulation, signaling pathway etc. Therefore, BRD7 may exert its functions by mediating differential expression of these proteins.
authors.affiliation: Cancer Research Institute, Xiangya Medical college, Central South University, Changsha 410078, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Peng
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Liang
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Lv
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Li
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Li
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D016147:Genes, Tumor Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D015854:Up-Regulation; D000092468:Bromodomain Containing Proteins
chemical_list: D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D011506:Proteins; C485694:BRD7 protein, human; D000092468:Bromodomain Containing Proteins