Analysis of three variable number terminal repeat loci is sufficient to characterize the deoxyribonucleic acid fingerprints of a panel of human tumor cell lines.
Using primers for the MCT118, YNZ22, and COL2A1 loci in polymerase chain reaction analysis we could distinguish among the approximately 20 cell lines routinely maintained in our laboratory. We also demonstrated that the cell line NB-1691 (a neuroblastoma) and its xenograft had an identical number of repeats at two loci. Rh30 (a rhabdomyosarcoma) made resistant to rapamycin was identical to its parent line and to a subline that had reverted to sensitivity after it was cultured without rapamycin in the medium.
Anding
Allyson L
AL
Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Reiss
Tanika
T
Germain
Glen S
GS
D001483:Base Sequence; D045744:Cell Line, Tumor; D016172:DNA Fingerprinting; D017931:DNA Primers; D006801:Humans; D018598:Minisatellite Repeats; D020079:Terminal Repeat Sequences
D017931:DNA Primers