Mutational analysis of salvador gene in human carcinomas.
It is believed that clonal expansion and cancer growth is the result of the deregulation of proliferation and cell death. Recently, salvador, a molecule that can regulate both cell proliferation and cell death, was identified. It was also reported that human salvador (hWW45) is mutated in some cancer cell lines. However, there have been no data regarding salvador gene mutations in human cancer tissues. To explore the hypothesis that the salvador gene might be similarly mutated in human cancer tissues, we analyzed the entire coding region of the salvador gene for the detection of somatic mutations in a series of human cancer tissues, including carcinomas from stomach, colon, liver and lung. However, using SSCP analysis, no mutation in the coding and splicing regions could be detected in the cancers. The data presented here suggest that salvador is not frequently mutated in human carcinoma tissues and that the mutation might be tumor-type specific.
Yoo
Nam Jin
NJ
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. suhulee@catholic.ac.kr
Soung
Young Hwa
YH
Lee
Jong Woo
JW
Park
Won Sang
WS
Kim
Su Young
SY
Nam
Suk Woo
SW
Han
Jung Ho
JH
Kim
Sang Ho
SH
Lee
Jung Young
JY
Lee
Sug Hyung
SH
D000230:Adenocarcinoma / Q000235:genetics*; D018797:Cell Cycle Proteins; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D011506:Proteins; C415864:SAV1 protein, human