title: Inhibitory effects of c9, t11-conjugated linoleic acid on invasion of human gastric carcinoma cell line SGC-7901.
abstract: OBJECTIVE
  To investigate the effect of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the invasion of human gastric carcinoma cell line and its possible mechanism of preventing metastasis.
  
  
  METHODS
  Using reconstituted basement membrane invasion, chemotaxis, adhesion, PAGE substrate zymography and RT-PCR assays, we analyzed the abilities of invasion, direct migration, adhesion of intracellular matrix, as well as the activity of type IV collagenase and expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 mRNA in SGC-7901 cells which were treated with gradually increased concentrations (25, 50, 100 and 200 micromol/L) of c9, t11-CLA for 24 h.
  
  
  RESULTS
  At the concentrations of 200 micromol/L, 100 micromol/L and 50 micromol/L, c9,t11-CLA suppressed the invasion of SGC-7901 cells into the reconstituted basement membrane by 53.7 %, 40.9 % and 29.3 %, respectively, in comparison with the negative control. Only in the 200 micromol/L c9,t11-CLA group, the chemotaxis of SGC-7901 cells was inhibited by 16.0 % in comparison with the negative control. C9,t11-CLA also could inhibit the adhesion of SGC-7901 cells to laminin, fibronectin and Matrigel, increase the expression of TIMP-1 and TIMP-2 mRNA, and reduce type IV collagenase activities in the serum-free medium supernatant of SGC-7901 cells.
  
  
  CONCLUSIONS
  c9,t11-CLA can inhibit the invasion of SGC-7901 cells at multiple procedures in tumor metastasis cascade, which may be associated with the induction of TIMP-1 and TIMP-2 mRNA expression.
authors.affiliation: Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 199 Dongdazhi Street, Nangang District, Harbin 150001, Heilongjiang Province, China. bingqingchen@sina.com
authors.forename: Bing-Qing
authors.initials: BQ
authors.lastname: Chen
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Yang
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Gao
authors.forename: Jia-Ren
authors.initials: JR
authors.lastname: Liu
authors.forename: Ying-Ben
authors.initials: YB
authors.lastname: Xue
authors.forename: Xuan-Lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zheng
authors.forename: Jing-Shu
authors.initials: JS
authors.lastname: Zhang
authors.forename: Rui-Hai
authors.initials: RH
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000503:physiopathology; D002633:Chemotaxis / Q000187:drug effects; D006801:Humans; D008041:Linoleic Acids / Q000494:pharmacology*; D044243:Linoleic Acids, Conjugated; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology* / Q000503:physiopathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C046938:9,11-linoleic acid