title: Secretory phospholipases A2 induce neurite outgrowth in PC12 cells.
abstract: sPLA(2)s (secretory phospholipases A(2)) belong to a broad and structurally diverse family of enzymes that hydrolyse the sn -2 ester bond of glycerophospholipids. We previously showed that a secreted fungal 15 kDa protein, named p15, as well as its orthologue from Streptomyces coelicolor (named Scp15) induce neurite outgrowth in PC12 cells at nanomolar concentrations. We report here that both p15 and Scp15 are members of a newly identified group of fungal/bacterial sPLA(2)s. The phospholipid-hydrolysing activity of p15 is absolutely required for neurite outgrowth induction. Mutants with a reduced PLA(2) activity exhibited a comparable reduction in neurite-inducing activity, and the ability to induce neurites closely matched the capacity of various p15 forms to promote fatty acid release from live PC12 cells. A structurally divergent member of the sPLA(2) family, bee venom sPLA(2), also induced neurites in a phospholipase activity-dependent manner, and the same effect was elicited by mouse group V and X sPLA(2)s, but not by group IB and IIA sPLA(2)s. Lysophosphatidylcholine, but not other lysophospholipids, nor arachidonic acid, elicited neurite outgrowth in an L-type Ca(2+) channel activity-dependent manner. In addition, p15-induced neuritogenesis was unaffected by various inhibitors that block arachidonic acid conversion into bioactive eicosanoids. Altogether, these results delineate a novel, Ca(2+)- and lysophosphatidylcholine-dependent neurotrophin-like role of sPLA(2)s in the nervous system.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakashima
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ikeno
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kuwana
authors.forename: Angelo
authors.initials: A
authors.lastname: Bolchi
authors.forename: Simone
authors.initials: S
authors.lastname: Ottonello
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Arioka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001514:Bee Venoms / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002454:Cell Differentiation; D005227:Fatty Acids / Q000378:metabolism; D005658:Fungi / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D016716:PC12 Cells; D010741:Phospholipases A / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D001514:Bee Venoms; D005227:Fatty Acids; C476805:Scp15 protein, Streptomyces coelicolor; D010741:Phospholipases A; D002118:Calcium