Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.
Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression of ToxA, ToxB and Cpe fragments either as individual components or as a hybrid multidomain (ToxA-ToxB-Cpe) protein. Rabbit monospecific sera raised against individual peptides reacted with the chimeric product indicating that the corresponding antigenic determinants were correctly expressed on the hybrid molecule. Furthermore, mice immunized with the fusion protein produced antibodies specific to each of the three separate components. These data suggest that the constructed three-domain molecule could be used in future studies for development of a vaccine against enteric clostridial diseases.
Belyi
Iouri F
IF
Laboratory of Molecular Pathogenesis, Gamaleya Research Institute of Epidemiology and Microbiology, ulitsa Gamalei 18, Moscow 123098, Russia. belyi@riem.ru
Varfolomeeva
Nina A
NA
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics* / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D016360:Clostridioides difficile / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D003016:Clostridium perfringens / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D007114:Immunization; D051379:Mice; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; D011993:Recombinant Fusion Proteins; C035598:enterotoxin, Clostridium; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile