Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae.
Epidemic Vibrio cholerae contain a large essential virulence gene cluster called the Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded mop gene but this mutant was hypervirulent and reactogenic in rabbit ileal loops. In this paper, we report in vitro studies showing that independent Mop mutants of strain 3083 are significantly attenuated (approximately 40-fold) in cholera toxin (CT) production and have significantly increased motility and biofilm forming ability but appear to be unaffected in TcpA, hemagglutinin protease and hemolysin compared to their parent. The 3083 Mop mutant showed a 100-fold decrease in its in vivo intestinal colonization ability in the infant mouse competition assays. While reverse transcription polymerase chain reaction and phenotypic studies of a mop plasmid in both mutant and wild-type backgrounds suggest Mop is expressed by the plasmid, the differences in CT and biofilm formation could not be restored in any of the mutants. The inability to complement the Mop mutants in trans may be due either to the selection of secondary mutations or to mop possibly being part of an operon. Our findings that Mop is associated with CT, motility, biofilm formation and intestinal colonization support a hypothesis in which Mop has a pleiotropic role in the pathogenesis and persistence of epidemic V. cholerae.
Zhang
Dalin
D
Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Rajanna
Chythanya
C
Sun
Weiyun
W
Karaolis
David K R
DK
D000818:Animals; D018441:Biofilms / Q000254:growth & development; D015153:Blotting, Western; D002448:Cell Adhesion / Q000235:genetics; D002772:Cholera Toxin / Q000096:biosynthesis; D036781:Fimbriae Proteins / Q000096:biosynthesis; D015870:Gene Expression; D005816:Genetic Complementation Test; D006460:Hemolysin Proteins / Q000096:biosynthesis; D007422:Intestines / Q000382:microbiology; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D009068:Movement; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D006460:Hemolysin Proteins; C085807:TcpA protein, Vibrio cholerae; D037521:Virulence Factors; D036781:Fimbriae Proteins; D002772:Cholera Toxin; D008666:Metalloendopeptidases; C470925:Mop protein, Vibrio cholerae; C039814:hemagglutinin-protease