Optimization stacking by transient pseudo-isotachophoresis for capillary electrophoresis: example analysis of plasma glutathione.
Low concentrations of both reduced form glutathione (GSH) and oxidized form glutathione (GSSG) in diabetic nephropathy (DN) patient's plasma were measured with transient pseudo-isotachophoresis. The plasma samples were deproteined with acetonitrile and centrifuged. The method was performed at constant voltage of 5 kV using a 300 mM borate buffer (pH 8.0), with a fused-silica capillary of 21 cm x 75 microm. The sample length can reach 25% of the efficient length of the capillary, and the sensitivities of GSH and GSSG increased 15-20-fold. The method was also systematically optimized, and the results show that this type of stacking offers good repeatability for routine clinical assay of glutathione in DN plasmas.
Kong
Yu
Y
Institute of Elemento-Organic Chemistry, Nankai University, 94 Weijin Road, 300071 Tianjin, China.
Zheng
Ning
N
Zhang
Zhichao
Z
Gao
Ruyu
R
D002138:Calibration; D003928:Diabetic Nephropathies / Q000097:blood; D004586:Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005978:Glutathione / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005978:Glutathione