title: The properties of acidic compartments in developing schistosomula of Schistosoma mansoni.
abstract: A variety of fluorescent probes have been used to study the acidic compartments in cercariae and schistosomula of Schistosoma mansoni. Freshly transformed schistosomula treated with the LysoTracker Red dye specific for lysosomes showed large acid-containing compartments (0.5-10 microm in size). The uptake of the dye is an energy-dependent process that depends on the metabolic activity of schistosomula. The compartments were quantified individually with respect to area, quantity of fluorescence and the total number/schistosomulum. Under normal conditions these compartments were not found in untreated cercariae, but appeared in cercariae slightly damaged by poly-L-lysine. The formation of these compartments seemed to be related to the development of cercariae into schistosomula as the number of compartments and uptake of fluorescence increased with time after transformation. Also, the method of transformation as well as the in vitro incubation of the parasite affected the percentage area of compartments/schistosomulum. Acid phosphatase enzyme activity was assessed using an endogenous phosphatase probe. Living and fixed schistosomula displayed the presence of enzyme activity in compartments of the same size and distribution as the acid-rich compartments. This was confirmed by histochemical staining showing deposition of enzyme-generated lead at the sites of phosphatase activity. We suggest that the development of acidic compartments is important during the transformation process or as a consequence of damage.
authors.affiliation: Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK. bhaa3f@udcf.gla.ac.uk
authors.forename: B H
authors.initials: BH
authors.lastname: Al-Adhami
authors.forename: J
authors.initials: J
authors.lastname: Thornhill
authors.forename: A
authors.initials: A
authors.lastname: Akhkha
authors.forename: M J
authors.initials: MJ
authors.lastname: Doenhoff
authors.forename: J R
authors.initials: JR
authors.lastname: Kusel
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D010744:Phosphoric Monoester Hydrolases; D012550:Schistosoma mansoni / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases