A non-mitochondrial carboxylase, related to glutamate action is synthesized in the retina of the chick embryo.
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot of chick retinal proteins at 21 days after intravitreal injection with 5 or 10 microM/eye Glu showed decreases in 37, 42, 53 and 57 kDa proteins and increases in 35, 72 and >94 kDa proteins. These proteins were carboxylases except for the 35 and 37 kDa proteins. With > or =15 microM/eye Glu, non-specific loss of retinal proteins was observed. In embryonic retinas, the 42 kDa protein was seen a few days before hatching, with biotin incorporation on days 3-6 after hatching. Immunohistochemistry indicated that this protein was a component of both the inner nuclear layer and the photoreceptor. Immunocytochemistry located it to the cell surface.
Sattayasai
Nison
N
Faculty of Science, Khon Kaen University, 40002 Khon Kaen, Thailand. nison@kku.ax.th
Sattayasai
Jintana
J
Daduang
Sakda
S
Chahomchuen
Thippayarat
T
Ketkaew
Somporn
S
Puchongkavarin
Hathairat
H
D000818:Animals; D000831:Animals, Newborn; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D002474:Cell-Free System / Q000201:enzymology; D002642:Chick Embryo / Q000201:enzymology* / Q000254:growth & development; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005136:Eye Proteins / Q000096:biosynthesis* / Q000378:metabolism; D018698:Glutamic Acid / Q000008:administration & dosage / Q000502:physiology*; D007150:Immunohistochemistry; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D009924:Organ Culture Techniques; D012160:Retina / Q000196:embryology / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D014822:Vitreous Body
D005136:Eye Proteins; D018698:Glutamic Acid; D002262:Carboxy-Lyases