T helper 2 immunity to hepatitis B surface antigen primed by gene-gun-mediated DNA vaccination can be shifted towards T helper 1 immunity by codelivery of CpG motif-containing oligodeoxynucleotides.
Gene-gun-mediated DNA immunization usually induces predominant T helper 2 (Th2) type immune response. As oligodeoxynucleotides (ODN)-containing unmethylated CpG motifs can activate the innate immune system in a Th1-biased way, the potential of codelivery of CpG motifs-containing ODN (CpG-ODN) with plasmid DNA to switch the gene-gun-mediated Th2 immune response was evaluated in this study. Here we show that codelivery of CpG-ODN with plasmid DNA at certain ratio (10/1) can enhance the Th1 humoral and cell-mediated immune responses in gene-gun-mediated DNA immunization in BALB/c mice, including increasing the hepatitis B surface antigen-specific total immunoglobulin G (IgG), IgG2a subclass, cytotoxic T-cell lymphocyte activity as well as interferon-gamma (IFN-gamma) secretion. Taken together, these results demonstrate that codelivery of CpG-ODN with recombinant plasmid DNA by gene gun can shift the gene-gun-mediated DNA immune response from Th2 towards Th1.
Zhou
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Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai, China.
Zheng
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Liu
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Xiang
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Yuan
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Z
D000276:Adjuvants, Immunologic; D000818:Animals; D019470:Biolistics; D004279:DNA, Viral / Q000008:administration & dosage / Q000276:immunology*; D015226:Dinucleoside Phosphates / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006046:Gold / Q000008:administration & dosage / Q000378:metabolism; D006509:Hepatitis B / Q000276:immunology / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000097:blood / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007132:Immunoglobulin Isotypes; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009841:Oligonucleotides / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D012867:Skin / Q000276:immunology / Q000648:ultrastructure; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
D000276:Adjuvants, Immunologic; D004279:DNA, Viral; D015226:Dinucleoside Phosphates; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007132:Immunoglobulin Isotypes; D009841:Oligonucleotides; D019444:Vaccines, DNA; C018309:guanylyl-(3'-5')-guanosine; D006046:Gold; D007371:Interferon-gamma