title: Frameshift mutations in infectious cDNA clones of Citrus tristeza virus: a strategy to minimize the toxicity of viral sequences to Escherichia coli.
abstract: The advent of reverse genetics revolutionized the study of positive-stranded RNA viruses that were amenable for cloning as cDNAs into high-copy-number plasmids of Escherichia coli. However, some viruses are inherently refractory to cloning in high-copy-number plasmids due to toxicity of viral sequences to E. coli. We report a strategy that is a compromise between infectivity of the RNA transcripts and toxicity to E. coli effected by introducing frameshift mutations into "slippery sequences" near the viral "toxicity sequences" in the viral cDNA. Citrus tristeza virus (CTV) has cDNA sequences that are toxic to E. coli. The original full-length infectious cDNA of CTV and a derivative replicon, CTV-DeltaCla, cloned into pUC119, resulted in unusually limited E. coli growth. However, upon sequencing of these cDNAs, an additional uridinylate (U) was found in a stretch of U's between nts 3726 and 3731 that resulted in a change to a reading frame with a stop codon at nt 3734. Yet, in vitro produced RNA transcripts from these clones infected protoplasts, and the resulting progeny virus was repaired. Correction of the frameshift mutation in the CTV cDNA constructs resulted in increased infectivity of in vitro produced RNA transcripts, but also caused a substantial increase of toxicity to E. coli, now requiring 3 days to develop visible colonies. Frameshift mutations created in sequences not suspected to facilitate reading frame shifting and silent mutations introduced into oligo(U) regions resulted in complete loss of infectivity, suggesting that the oligo(U) region facilitated the repair of the frameshift mutation. Additional frameshift mutations introduced into other oligo(U) regions also resulted in transcripts with reduced infectivity similarly to the original clones with the +1 insertion. However, only the frameshift mutations introduced into oligo(U) regions that were near and before the toxicity region improved growth and stability in E. coli. These data demonstrate that, when hosts are sufficiently susceptible for infection by transcripts of reduced specific infectivity, introduction of frameshift mutations at "slippery sequences" near toxic regions of viral cDNAs can be used as an additional strategy to clone recalcitrant viral sequences in high-copy-number plasmids for reverse genetics.
authors.affiliation: Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred 33850, USA.
authors.forename: Tatineni
authors.initials: T
authors.lastname: Satyanarayana
authors.forename: Siddarame
authors.initials: S
authors.lastname: Gowda
authors.forename: María A
authors.initials: MA
authors.lastname: Ayllón
authors.forename: William O
authors.initials: WO
authors.lastname: Dawson
mesh_terms: D002957:Citrus / Q000821:virology*; D003001:Cloning, Molecular; D017790:Closterovirus / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012093:Replicon; D014169:Transformation, Bacterial
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral