Immunohistochemical localization of Bis protein in the rat central nervous system.
We studied the distribution of Bis (Bcl-2 interacting death suppressor) protein in the adult rat brain and spinal cord using immunohistochemistry. Immunoreactivity was observed in specific neuronal populations in distinct nuclei. The most intensely labeled cells were associated with the motor system, including most cranial nerve motor nuclei, Purkinje cells of the cerebellum, the red nucleus, and the ventral motor neurons of the spinal cord. Bis protein was also expressed in several structures associated with the ventricular system, including the subventricular zone of the lateral ventricle and its rostral extension, in the subcommissural organ, and in tanycytes, radial glial cells in the hypothalamus. Using double-labeling techniques, Bis-immunoreactive cells in the rostral migratory stream, coexpressing Bcl-2, were confirmed as glial fibrillary acidic protein-positive astrocytes comprising the glial tubes. The widespread distribution of Bis suggests that this protein has broader functions in the adult rat central nervous system than previously thought, and that it could be associated with a particular role in the rostral migratory system.
Park
Hyun-Jung
HJ
Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, 137-701 Seoul, Korea.
Choi
Jeong-Sun
JS
Chun
Myung-Hoon
MH
Chung
Jin-Woong
JW
Jeon
Mi-Hee
MH
Lee
Jeong-Hwa
JH
Lee
Mun-Yong
MY
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology* / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D004027:Diencephalon / Q000166:cytology / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution
D019253:Proto-Oncogene Proteins c-bcl-2