Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein.
A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.
Earl
Patricia L
PL
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.
Americo
Jeffrey L
JL
Moss
Bernard
B
D000818:Animals; D002452:Cell Count; D005434:Flow Cytometry / Q000379:methods*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis*; D009500:Neutralization Tests / Q000379:methods*; D011817:Rabbits; D012900:Smallpox Vaccine / Q000276:immunology*; D014611:Vaccination; D014616:Vaccinia virus / Q000235:genetics*
D008164:Luminescent Proteins; D012900:Smallpox Vaccine; D049452:Green Fluorescent Proteins