Analysis of genetic variation in Ganoderma lucidum after space flight.
A modified CTAB method was used in the extraction of total cellular DNA of Ganoderma lucidum. Four strains Cx, Ch, C3 and C4, and their counterparts, four space flown strains Sx, Xh, S3 and S4, were analysed by amplified fragment length polymorphism (AFLP) with several primer combinations. Polymorphic bands were detected between Sx and Cx, S3 and C3, respectively. Somatic incompatibility tests further confirmed their heterogeneity. However, no disparity between Sh and Ch, S4 and C4 was detectable. The results suggest that spaceflight may be used to accelerate breeding of Ganoderma lucidum strains for commercial cultivation.
Qi
Jian-Jun
JJ
Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Beijing, China.
Ma
Rong-Cai
RC
Chen
Xiang-Dong
XD
Lan
Jin
J
D000383:Agriculture / Q000379:methods; D001947:Breeding; D004271:DNA, Fungal / Q000032:analysis*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D020940:Reishi / Q000235:genetics* / Q000254:growth & development*; D013026:Space Flight; D014893:Weightlessness
D004271:DNA, Fungal