Sphingosine-1-phosphate stimulates motility and invasiveness of human glioblastoma multiforme cells.
Sphingosine-1-phosphate (S1P) is a bioactive lipid which is a potent mitogen for glioblastoma multiforme cells. Here we show that S1P also potently enhances the in vitro motility of glioblastoma cells by signaling through receptors coupled to G(i/o) proteins. Moreover, S1P also enhanced in vitro invasion of glioblastoma cells through Matrigel. S1P had no effect on matrix metalloproteinase secretion but did enhance glioblastoma cell adhesion. S1P is present at high levels in brain tissue. Thus it is possible that autocrine or paracrine signaling by S1P through its G protein-coupled receptors enhances both glioma cell proliferation and invasiveness.
Van Brocklyn
James R
JR
Department of Pathology, The Ohio State University, 4164 Graves Hall, 333 W. 10th Ave., Columbus, OH 43210, USA. van-brocklyn.1@osu.edu
Young
Nicholas
N
Roof
Rosemary
R
D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D019204:GTP-Binding Proteins / Q000378:metabolism; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D008246:Lysophospholipids; D009361:Neoplasm Invasiveness; D015398:Signal Transduction / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D019204:GTP-Binding Proteins; D013110:Sphingosine