Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms.
Identification, localization and partial biochemical characterization of actins expressed in the larval stage of the cestode parasite Taenia solium has been carried out. Frozen tissue sections of cysticerci, the larval stage of this parasite, were reacted with rhodamine-phalloidin, parasite actin was purified by polymerization in the presence of K(+), Mg(++) and ATP actin was analyzed by SDS-PAGE and two-dimensional gel electrophoresis, and immunoblotting of actin was performed in PVDF membranes and with commercial anti-actin monoclonal antibodies. Parasitic tissues showed different fibrous actin fluorescence patterns, which correlated with the expression of isoactins. Purified globular actin had a similar molecular mass to rabbit commercial actin (approximately 44 kDa). Actin was resolved into seven isoforms, indicating a family of actin genes.
Ambrosio
Javier R
JR
Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, D.F. 04510 Mexico, Mexico. jrah@servidor.unam.mx
Reynoso-Ducoing
Olivia
O
Hernández-Sanchez
Hugo
H
Correa-Piña
David
D
González-Malerva
Laura
L
Cruz-Rivera
Mayra
M
Flisser
Ana
A
D000199:Actins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013552:Swine / Q000469:parasitology; D041201:Taenia solium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D014018:Tissue Distribution
D000199:Actins; D020033:Protein Isoforms