[Determination of clozapine in blood serum using high pressure liquid chromatography (HPLC)].
For the simultaneous determination of clozapine in presence of its metabolite in serum a sensitive reversed phase HPLC method was used. The mobile phase was a mixture of acetonitril-methanol-phosphoric buffer pH 4.5 (5:4:1). The solvent for extracting clozapine from serum was diethyl ether. Dezipramine hydrochloride was used as an internal standard. The method is sufficiently sensitive (25 ng) and reproducible for clinical and pharmacokinetic studies.
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Katedra i Zakład Chemii Leków Akademii Medycznej w Lublinie, ul. Okopowa 1, 20-022 Lublin.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003024:Clozapine / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
D003024:Clozapine
Oznaczanie klozapiny w surowicy krwi metoda wysokosprawnej chromatografii cieczowej (HPLC).