Identification and characterization of chicken interleukin-16 cDNA.
Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded protein, predicted to consist of 607 amino acids, showed 86% sequence identity to duck pro-IL-16 and 49-52% identity to various mammalian homologues. By Northern blot analysis, IL-16 transcripts were identified in chicken lymphoid tissues but none of the non-lymphoid tissues examined. A recombinant protein containing the 149 C-terminal amino acids of pro-IL-16, expressed in COS-7 cells, showed chemoattractant activity for splenic lymphocytes.
Min
Wongi
W
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Service, U.S. Department of Agriculture, Bldg 1040, BARC-East, Beltsville, MD 20705, USA.
Lillehoj
Hyun Soon
HS
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000276:immunology*; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D019410:Interleukin-16 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014018:Tissue Distribution
D002630:Chemotactic Factors; D018076:DNA, Complementary; D019410:Interleukin-16; D011994:Recombinant Proteins