title: LC determination of impurities in azithromycin tablets.
abstract: A LC method with UV detection for determining azithromycin impurities in tablets as pharmaceutical form has been developed. It is to be employed in routine and stability tests. A linear gradient elution was employed starting with 47% A and 53% B to reach 28% A and 72% B at 48 min. Mobile phase A was KH2PO4 10 mM (H2O) at pH 7.00. B was a mixture methanol:acetonitrile 1:1 (v/v). UV detection was performed at 210 nm. The chromatographic column was Phenomenex Synergi MAX-RP 4 microm 250 x 460 mm kept at 50 degrees C. Six impurities were separated and identified and it was possible to quantify five out of the six with reasonable accuracy and precision.
authors.affiliation: Facultad de CC Experimentales y de la Salud. Universidad San Pablo-CEU, Urbanización Montepri;ncipe, Ctra. Boadilla del Monte, km 5,3-28668 Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Miguel
authors.forename: C
authors.initials: C
authors.lastname: Barbas
mesh_terms: D000143:Acids; D000900:Anti-Bacterial Agents / Q000032:analysis*; D017963:Azithromycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006358:Hot Temperature; D008027:Light; D016877:Oxidants / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D000143:Acids; D000900:Anti-Bacterial Agents; D016877:Oxidants; D013607:Tablets; D017963:Azithromycin