title: Reevaluation of production of paralytic shellfish toxin by bacteria associated with dinoflagellates of the Portuguese coast.
abstract: Paralytic shellfish toxins (PSTs) are potent neurotoxins produced by certain dinoflagellate and cyanobacterial species. The autonomous production of PSTs by bacteria remains controversial. In this study, PST production by two bacterial strains, isolated previously from toxic dinoflagellates, was evaluated using biological and analytical methods. Analyses were performed under conditions determined previously to be optimal for toxin production and detection. Our data are inconsistent with autonomous bacterial PST production under these conditions, thereby challenging previous findings for the same strains.
authors.affiliation: Laboratório de Microbiologia e Ecotoxicologia, Instituto Nacional de Saúde Dr. Ricardo Jorge, 1649-016 Lisbon, Portugal. lme.cqh@insa.min-saude.pt
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Martins
authors.forename: Paula
authors.initials: P
authors.lastname: Alvito
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Tavares
authors.forename: Paulo
authors.initials: P
authors.lastname: Pereira
authors.forename: Gregory
authors.initials: G
authors.lastname: Doucette
authors.forename: Susana
authors.initials: S
authors.lastname: Franca
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004141:Dinoflagellida / Q000382:microbiology*; D008387:Marine Toxins / Q000302:isolation & purification*; D009498:Neurotoxins / Q000302:isolation & purification*; D011174:Portugal; D011549:Pseudomonas / Q000472:pathogenicity*; D044223:Pseudomonas stutzeri / Q000472:pathogenicity*; D012623:Seawater; D012758:Shellfish
chemical_list: D008387:Marine Toxins; D009498:Neurotoxins