title: cDNA cloning of the lysozyme of the white shrimp Penaeus vannamei.
abstract: Lysozyme, an antibacterial protein, has been implicated in innate immunity in invertebrates, but its activity in shrimp remained to be determined. We cloned the white shrimp lysozyme cDNA using a PCR strategy and detected its activity in haemocytes using a lytic-zone assay against Micrococcus luteus. The cloning was based on a reported EST (dbEST BE18831). The deduced amino acid sequence resulted in 150 amino with 46% identity to hen egg white lysozyme. RT-PCR was used to detect lysozyme mRNA in haemocytes. Analysis of the amino acid sequence of the shrimp lysozyme showed that it belongs to the C-type family of lysozymes. Furthermore, the lysozyme amino acid sequence contained extra residues at its C-terminus, which are characteristic of marine invertebrates. This information will be useful in future studies on the molecular mechanisms of immunity in marine invertebrates.
authors.affiliation: Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, P.O. Box 1735, Hermosillo, Sonora 83000, Mexico. rrs@cascabel.ciad.mx
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
authors.forename: Maria A
authors.initials: MA
authors.lastname: Islas-Osuna
authors.forename: Enrique
authors.initials: E
authors.lastname: de-la-Re-Vega
authors.forename: Jorge
authors.initials: J
authors.lastname: Hernández-López
authors.forename: Francisco
authors.initials: F
authors.lastname: Vargas-Albores
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D006434:Hemocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009113:Muramidase