title: DNA adducts in normal bladder tissue and bladder cancer risk.
abstract: Cigarette smoking is an established cause of bladder cancer. The direct relationship between smoking-induced DNA adducts in bladder cells and cancer risk at that site has, however, been poorly assessed. We therefore investigated the relationship between bladder cancer risk and levels of DNA adducts measured in normal bladder biopsies by 32P-post-labeling in a hospital-based case-control study of 59 bladder cancer patients and 45 controls submitted to surgery for prostatic hyperplasia or urinary incontinence. An approximately 2-fold risk for bladder cancer was found in individuals with an adduct level >14.8 (median among controls) compared with those with an adduct level < or =14.8 (OR = 1.9, 95% CI 0.8-4.3, P = 0.13). A dose-response relationship was also suggested (trend test, P = 0.13): compared with adduct levels below 13.5, the OR for bladder cancer was 1.7 (95% CI 0.6-4.6) for adduct levels between 13.5 and 18.5 and 2.2 (95% CI 0.8-6.1) for adduct levels >18.5. These findings provide some evidence that DNA adducts in bladder tissue might predict smoking-induced bladder cancer. Larger studies are still warranted to confirm these results.
authors.affiliation: INSERM and Evry University EMI 00-06, Statistical Methods and Genetic Epidemiology of Multifactorial Diseases, Evry, France. benhamou@evry.inserm.fr
authors.forename: Simone
authors.initials: S
authors.lastname: Benhamou
authors.forename: Agnès
authors.initials: A
authors.lastname: Laplanche
authors.forename: Bertrand
authors.initials: B
authors.lastname: Guillonneau
authors.forename: Arnaud
authors.initials: A
authors.lastname: Mejean
authors.forename: François
authors.initials: F
authors.lastname: Desgrandchamps
authors.forename: Catherine
authors.initials: C
authors.lastname: Schrameck
authors.forename: Valérie
authors.initials: V
authors.lastname: Degieux
authors.forename: François
authors.initials: F
authors.lastname: Perin
mesh_terms: D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D018736:DNA Adducts / Q000032:analysis*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D008297:Male; D010761:Phosphorus Radioisotopes; D011237:Predictive Value of Tests; D012016:Reference Values; D012307:Risk Factors; D012907:Smoking; D001743:Urinary Bladder / Q000502:physiology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D018736:DNA Adducts; D010761:Phosphorus Radioisotopes