[Expression of vascular cell adhesion molecule-1 in lung slices from antigen  sensitized rats and pharmacological modulation].
To investigate the expression of vascular cell adhesion molecule-1(VCAM-1) in lung slices from antigen  -sensitized rats and the modulation by drugs.



In isolated lung slices from ovalbumin(OVA)-sensitized rats, the relative expression of VCAM-1 was determined after drug treatment and OVA challenge.



The expression of VCAM-1 was enhanced in the sensitized rat lungs,and OVA challenge did not further increase the expression. Glycocorticosteroid dexamethasone and leukotriene cysLT receptor antagonist ONO-1078 inhibited the expression,but tachykinin NK-1 receptor antagonist SR-140333 had no such effect.



VCAM-1 expression is enhanced in the sensitized rat lungs, and antigen challenge does not further up regulate the expression. Anti-inflammatory drugs have different effects on VCAM-1 expression. Dexamethasone and ONO-1078, but not SR-140333, can inhibit the expression.
Yang
Xiao-hong
XH
Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Wei
Er-qing
EQ
D000818:Animals; D002867:Chromones / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008168:Lung / Q000737:chemistry*; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D010880:Piperidines / Q000494:pharmacology; D011812:Quinuclidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*
D002867:Chromones; D010880:Piperidines; D011812:Quinuclidines; D019010:Vascular Cell Adhesion Molecule-1; C085215:SR 140333; D003907:Dexamethasone; D010047:Ovalbumin; C047681:pranlukast