title: Physical properties of type I collagen extracted from fish scales of Pagrus major and Oreochromis niloticas.
abstract: Type I collagens were extracted from fish scales of Pagrus major and Oreochromis niloticas as a possible underutilized resource for medical materials. The fish scales were demineralized with EDTA and digested by pepsin. The resultant type I collagens contained more than 33.6% of glycine as the most abundant amino acid. The denaturation temperatures of the collagens from P. major and O. niloticas were 303 and 308K, respectively, both of which were relatively lower than that of porcine dermis collagen (314K). CD spectra indicated that the denaturation temperatures were dependent on the amount of hydroxyproline, rather than proline residues. Raman spectra also indicated that the relative intensities of Raman lines at 879 and 855cm(-1) assigned to Hyp and Pro rings were changed due to the contents of the imino acids. Significantly, the content of sulphur-containing methionine was higher in the fish scales than in porcine dermis. The enthalpy and entropy estimated from thermal analyses could be correlated to amino acid sequences (Gly-Pro-Hyp) of type I collagens and the number of methionine amino acid residues.
authors.affiliation: Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan. IKOMA.Toshiyuki@nims.go.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ikoma
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Junzo
authors.initials: J
authors.lastname: Tanaka
authors.forename: Dominic
authors.initials: D
authors.lastname: Walsh
authors.forename: Stephen
authors.initials: S
authors.lastname: Mann
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D024042:Collagen Type I / Q000737:chemistry* / Q000302:isolation & purification*; D005399:Fishes; D006909:Hydroxyproline / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011489:Protein Denaturation; D013059:Spectrum Analysis, Raman; D013696:Temperature
chemical_list: D024042:Collagen Type I; D011392:Proline; D006909:Hydroxyproline