Bilayered phospholipid micelles and capillary electrophoresis: a new additive for electrokinetic chromatography.
Phospholipid micelles and bilayered micelles (bicelles) were investigated as a new media for electrokinetic chromatography. The benefit to using these additives for micellar electrokinetic chromatography (MEKC) is the potential of a simple bilayer membrane model operated with fast analysis time, and low sample injection volumes. The system is used to separate peptides/protein and is tested with a series of beta-blockers. The results suggest that bicelle electrokinetic chromatography provides selectivity and holds potential as an alternative approach to modeling membrane phenomenon.
Holland
Lisa A
LA
Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA. lisa.holland@mail.wvu.edu
Leigh
Alyison M
AM
D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000737:chemistry; D009320:Atrial Natriuretic Factor / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D019075:Electrophoresis, Capillary; D013006:Growth Hormone / Q000032:analysis; D008051:Lipid Bilayers / Q000737:chemistry*; D008823:Micelles; D009211:Myoglobin / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010742:Phospholipid Ethers / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D013997:Time Factors
C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D000319:Adrenergic beta-Antagonists; D008051:Lipid Bilayers; D008823:Micelles; D009211:Myoglobin; D010455:Peptides; D010742:Phospholipid Ethers; D010743:Phospholipids; D011506:Proteins; D009320:Atrial Natriuretic Factor; D013006:Growth Hormone; D004134:Dimyristoylphosphatidylcholine