The 3'UTR of stem cell factor suppresses protein expression from a cotransfected vector.
One of the isoforms of stem cell factor (SCF) was isolated from an embryonic kidney library with the ability to suppress the expression of cotransfected gene. The responsible region resides in the SCF 3' untranslated region (UTR). The SCF 5'UTR also has repressive element with a smaller effect than 3'UTR. The mRNA expression level of the cotransfected gene was not markedly changed. These results suggest that the SCF 3'UTR should negatively regulate gene expression at a posttranscriptional level.
Hirokawa
Yoshifumi S
YS
Second Department of Pathology, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. ultray2k@clin.medic.mie-u.ac.jp
Watanabe
Masatoshi
M
Shiraishi
Taizo
T
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D019089:Stem Cell Factor / Q000235:genetics* / Q000378:metabolism*; D013489:Suppression, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
D002352:Carrier Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D019089:Stem Cell Factor