Trans-repression of beta-catenin activity by nuclear receptors.
The signaling/oncogenic activity of beta-catenin can be repressed by the activation of nuclear receptors such as the vitamin A, vitamin D, and androgen receptors. Although these receptors directly interact with beta-catenin and can sequester it away from its transcription factor partner T-cell factor, it is not known if this is the mechanism of trans-repression. Using several different promoter constructs and nuclear receptors and mammalian two-hybrid and mutation analyses we now show that interaction with the co-activator, p300, underlies the trans-repression of beta-catenin signaling by nuclear receptors and their ligands.
Shah
Salimuddin
S
The Lombardi Cancer Center and the Department of Oncology, Georgetown University School of Medicine, Washington, DC 20057, USA.
Hecht
Andreas
A
Pestell
Richard
R
Byers
Stephen W
SW
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D008024:Ligands; D008156:Luciferases / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015398:Signal Transduction; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D008024:Ligands; D009687:Nuclear Proteins; D015534:Trans-Activators; D018808:Transcription Factor AP-1; D051176:beta Catenin; D019938:Cyclin D1; D014212:Tretinoin; D008156:Luciferases