Microkinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques.
By tissue culture methods and with the use of phase contrast, interference color contrast, and time-lapse cinematographic equipment, the activity of a cytoplasmic organoid, termed the microkinetosphere, has been followed and correlated with pinocytosis. A transformation of several microkinetospheres by coherence and coalescence into the solitary VP satellite was observed in cells undergoing pinocytosis in serum nutrients. A correlation of both of these structures to cytoplasmic organoids described by others, notably with the electron microscope, and a hypothesis on the nature of the microkinetosphere and its transformation was presented.
D006367:HeLa Cells; D006801:Humans; D008858:Microscopy, Phase-Contrast; D010873:Pinocytosis; D044967:Serum; D046509:Tissue Culture Techniques
TISSUE CULTURE