Methods and principles of fixation by freeze-substitution.
D000096:Acetone; D017110:Freeze Substitution; D005615:Freezing; D006652:Histological Techniques; D006653:Histology; D008854:Microscopy, Electron; D009993:Osmium Tetroxide; D011309:Preservation, Biological; D012997:Solvents; D013696:Temperature
D012997:Solvents; D000096:Acetone; D009993:Osmium Tetroxide
HISTOLOGY