A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system.
A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OH-dG fraction in the first HPLC is precisely and automatically collected based on the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001331:Automation; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004563:Electrochemistry / Q000295:instrumentation; D006801:Humans; D016482:Urinalysis / Q000379:methods*
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine