title: Pathogenesis of poliovirus infection in PVRTg mice: poliovirus replicates in peritoneal macrophages.
abstract: The pathogenesis of poliovirus infection, responsible for the induction of a poliovirus-specific mucosal immune response following intraperitoneal (i.p.) inoculation of virus in mice transgenic for the poliovirus receptor (PVRTg mice), was studied. Following inoculation of poliovirus, replication was determined by increase in virus titre (TCID(50)) and by PCR of poliovirus-specific negative-strand RNA in peritoneal macrophages, mesenteric lymph nodes, Peyer's patches, duodenum, brain, kidney and liver. The presence of poliovirus antigens in several cell types was detected by immunolabelling. It was demonstrated that poliovirus replicated in the peritoneal macrophages of PVRTg mice, since the virus titre in peritoneal cells was increased compared to the titre in the inoculum. Negative-strand RNA was detected in these cells and most of the poliovirus-immunostained cells had the morphology of macrophages and expressed the macrophage-specific markers CD86 and M1/70 on their surface. Furthermore, in peritoneal lavage, poliovirus was also present in CD19(+) B cells, but not in dendritic or T cells. Moreover, poliovirus was detected in macrophage-like cells in the lamina propria of the intestine, but not in epithelial cells. Replication of poliovirus in mesenteric lymph nodes, Peyer's patches and brain was followed by excretion of virus in the faeces. This suggests that the virus is transported due to migration of macrophages from the peritoneal cavity to mesenteric lymph nodes and the lamina propria of Peyer's patches. It is likely that this route is responsible for the induction of virus-specific IgA in the gut.
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D017737:Macrophages, Peritoneal / Q000821:virology*; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D008565:Membrane Proteins; D012367:RNA, Viral; D011991:Receptors, Virus; C066100:poliovirus receptor