Stabilisation of eudesmane cation by tryptophan 334 during aristolochene synthase catalysis.
Analysis of the hydrocarbons produced during catalysis by mutants of aristolochene synthase from Penicillium roqueforti indicated that Trp 334 had a pivotal function for the efficient production of aristolochene from farnesylpyrophosphate most likely by stabilising the intermediate, eudesmane cation.
D002384:Catalysis; D002412:Cations; D007535:Isomerases / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry*; D045787:Sesquiterpenes, Eudesmane; D014364:Tryptophan / Q000737:chemistry*
D002412:Cations; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; C054277:aristolochene; C015872:eudesmane; C004808:farnesyl pyrophosphate; D014364:Tryptophan; D007535:Isomerases; C060050:aristolochene synthase