title: Plasticity in developing rat uterine sensory nerves: the role of NGF and TrkA.
abstract: In the present study we investigated the effects of infantile/prepubertal chronic oestrogen treatment, chemical sympathectomy with guanethidine and combined sympathectomy and chronic oestrogen treatment on developing sensory nerves of the rat uterus. Changes in sensory innervation were assessed quantitatively on uterine cryostat tissue sections stained for calcitonin gene-related peptide (CGRP). Uterine levels of NGF protein, using immunohistochemistry and ELISA, and mRNA, using Northern blots and in situ hybridization, were also measured. Finally, levels of TrkA NGF receptor in sensory neurons of T13 and L1 dorsal root ganglia (DRG), which supply the uterus, were assessed using densitometric immunohistochemistry. These studies showed that: (1) chronic oestrogen treatment led to an 83% reduction in the intercept density of CGRP-immunoreactive nerves; (2) sympathectomy had no effect on the density of uterine sensory nerves or on the pattern of oestrogen-induced changes; (3) NGF mRNA and protein increased following sympathectomy or chronic oestrogen treatment; and (4) oestrogen produced increased intensity of labelling (28%) for TrkA receptors in small-diameter sensory neurons, but decreased labelling (13%) in medium-sized neurons, which represent the large majority of the DRG neurons supplying the upper part of the uterine horn. Contrary to expectations, increased levels of NGF after sympathectomy and oestrogen treatment did not lead to increased sensory innervation of the uterus. The possibility that alterations in neuronal levels of TrkA contribute to the lack of response of uterine sensory nerves to the oestrogen-induced increase in NGF levels is discussed.
mesh_terms: D018663:Adrenergic Agents / Q000494:pharmacology; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005727:Ganglia, Spinal / Q000033:anatomy & histology / Q000166:cytology; D006145:Guanethidine / Q000494:pharmacology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020932:Nerve Growth Factor / Q000378:metabolism*; D009473:Neuronal Plasticity; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020917:Receptor, trkA / Q000378:metabolism*; D013563:Sympathectomy, Chemical; D014599:Uterus / Q000254:growth & development / Q000294:innervation* / Q000378:metabolism
chemical_list: D018663:Adrenergic Agents; D004967:Estrogens; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D015740:Calcitonin Gene-Related Peptide; D006145:Guanethidine