title: Model description of bacterial 3-methylcatechol production in one- and two-phase systems.
abstract: Pseudomonas putida MC2 produces 3-methylcatechol from toluene in aqueous medium. A second phase of 1-octanol may improve total product accumulation. To optimise the design of such a biphasic process, a process model was developed, both for one- and two-phase applications. The insights obtained by the model predictions showed the importance of different process parameters (like growth substrate concentration and partition coefficient) on growth of biomass, accumulation of 3-methylcatechol and processing time. For future applications, the process model can be used to ensure enough extraction capacity from aqueous to octanol phase. It is a useful tool to define the optimum process conditions, depending on the desired optimisation parameter: product concentration or processing time.
mesh_terms: D020003:1-Octanol / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D002396:Catechols / Q000302:isolation & purification / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D003198:Computer Simulation; D008954:Models, Biological; D016958:Pseudomonas putida / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014050:Toluene / Q000378:metabolism*
chemical_list: D002396:Catechols; C031136:3-methylcatechol; D014050:Toluene; D020003:1-Octanol