GnRH-PAP hormonotoxin targets cytotoxicity to prostate cancer cell lines.
Pokeweed antiviral protein (PAP) is a plant-derived, highly potent ribosome inactivating protein that causes inhibition of protein translation and rapid cell death. We have previously described potent cytotoxic activity of a GnRH (gonadotropin-releasing hormone) receptor-targeted conjugate protein (GnRH-PAP) and demonstrated that cytotoxicity depended on the number of GnRH receptors and the duration of exposure. Here, we demonstrate that the GnRH-PAP conjugate was cytotoxic to three different prostate cancer cell lines, supporting the feasibility of using such hormonotoxins as novel therapeutics for hormone-responsive cancers such as prostate cancer.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D016466:CHO Cells; D002277:Carcinoma / Q000378:metabolism / Q000503:physiopathology*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004338:Drug Combinations; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor; D005240:Feasibility Studies; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D006801:Humans; D008297:Male; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000008:administration & dosage*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1; D014410:Tumor Stem Cell Assay
D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D010940:Plant Proteins; D012333:RNA, Messenger; D011966:Receptors, LHRH; D054789:Ribosome Inactivating Proteins, Type 1; D007987:Gonadotropin-Releasing Hormone; C035029:LHRH, Lys(6)-; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein