Propagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodon.
Yellow head virus is a highly pathogenic agent that can cause a fatal disease in several species of penaeid shrimps. Using a primary cell culture and an in vitro quantal assay (TCID50), this study sought to determine the propagation profile of yellow head virus after inoculation at a low multiplicity of infection in the lymphoid tissue (oka organ) of Penaeus monodon. Detectable levels of virus were present as early as 24 h post-inoculation. Maximal viral yields were reached by 4 d post-infection, approximately 24 h after the onset of a detectable cytopathic effect, which was normally observable at 3 d post-inoculation. The methodology provides a useful tool for studying yellow head virus-host-cell interactions.
D000818:Animals; D016367:Baculoviridae / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D008214:Lymphocytes / Q000166:cytology / Q000821:virology*; D033561:Penaeidae / Q000821:virology*