Photostability studies on the furosemide-triamterene drug association.
The photostability of the diuretic drugs triamterene and furosemide, individually and combined, was evaluated. Spectrophotometric, spectrofluorimetric and chromatographic (HPLC) methods were applied to monitor the drug photodegradation. Furosemide was confirmed to be highly photolable in both pH 7.4 solution and methanol. Differently triamterene proved to be highly fluorescent (emission quantum yield: 0.9 in methanol and 0.8 in pH 7.4 solution), but essentially photostable (photochemical reaction quantum yield: congruent with 5 x 10(-4)) under exposure at 365 and 313 nm radiations. When the combined drugs in pH 7.4 solutions were exposed to 365 nm radiations a significant photoprotective effect of triamterene on furosemide was observed. The photoreactivity of the drugs was exploited to develop an HPLC method involving a post-column on-line photochemical derivatization useful to confirm the analyte identity in a commercial dosage form (tablets). The commercial product, containing the combined drugs, proved to be photostable also after long (65 h) light exposure.
D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004347:Drug Interactions; D004355:Drug Stability; D005665:Furosemide / Q000737:chemistry / Q000528:radiation effects*; D007700:Kinetics; D019999:Pharmaceutical Solutions; D010777:Photochemistry; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets; D013997:Time Factors; D014223:Triamterene / Q000737:chemistry / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004338:Drug Combinations; D019999:Pharmaceutical Solutions; D012997:Solvents; D013607:Tablets; D005665:Furosemide; D014223:Triamterene