Use of phosphorus-32 in microassay for nucleic acid synthesis in Escherichia coli.
A method was developed to facilitate studies of ribonucleic acid and deoxyribonucleic acid synthesis in growing bacterial cultures of small volume. The nucleic acid fractions were separated by means of a modified Schmidt-Thannhauser procedure applied to micro quantities of P(32)-labeled cells collected on membrane filters. The validity and usefulness of the method are discussed.
D004926:Escherichia coli / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism*; D010758:Phosphorus; D012313:RNA; D011868:Radioisotopes
D009696:Nucleic Acids; D011868:Radioisotopes; D010758:Phosphorus; D012313:RNA
ESCHERICHIA COLI/metabolism; NUCLEIC ACIDS/metabolism