Specific inhibition of wheal-and-erythema responses with univalent haptens and univalent antibody fragments.
Wheal-and-erythema responses were studied in normal human volunteers and in a single human subject who is sensitive to the 2,4-dinitrophenyl group. In the normal subjects, reactive skin sites were established by intradermal injection of purified rabbit antibody specific for the 2,4-dinitrophenyl group. In both the active and passively sensitized subjects, wheal-and-erythema was elicited by intradermal injection of a 2,4-dinitrophenyl protein, but not by injection of the same conjugate mixed with certain low molecular weight 2,4-dinitrophenyl haptens or with univalent fragments split by papain from anti-2,4-dinitrophenyl antibody. The latter fragments, unlike intact, bivalent, antibody, do not sensitize normal human skin sites. From these and other observations it is concluded that the wheal-and-erythema response in human skin requires mutually multivalent antigen and antibody. This requirement suggests that multimolecular complexes, containing at least 2 antigen and 2 antibody molecules, are essential in the pathogenesis of this allergic response.
D000818:Animals; D000906:Antibodies; D019138:Antibodies, Blocking; D000941:Antigens; D004890:Erythema; D006241:Haptens; D006801:Humans; D006967:Hypersensitivity; D007128:Immunoglobulin Fragments; D011817:Rabbits; D012867:Skin; D012879:Skin Physiological Phenomena
D000906:Antibodies; D019138:Antibodies, Blocking; D000941:Antigens; D006241:Haptens; D007128:Immunoglobulin Fragments
ANTIBODIES; SKIN/physiology