title: Serum concentrations of pepsinogen A in healthy dogs after food deprivation and after feeding.
abstract: OBJECTIVE
  To develop and validate an ELISA for measurement of serum canine pepsinogen A (cPG A) as a diagnostic marker of gastric disorders in dogs and to measure serum cPG A in healthy dogs after food deprivation and after feeding.
  
  
  METHODS
  Sera from 72 healthy dogs.
  
  
  METHODS
  A sandwich ELISA was developed and validated. The reference range for serum concentrations of cPG A was determined in 64 healthy dogs. Postprandial changes in serum concentrations of cPG A were evaluated in 8 healthy dogs.
  
  
  RESULTS
  Assay sensitivity was 18 microg/L, and the maximum detectable concentration was 1,080 microg/L. The observed-to-expected ratio (O:E) for 3 serial dilutions of 3 serum samples ranged from 69.3 to 104.1%. The O:E for 3 serum samples spiked with 8 concentrations of cPG A ranged from 58.8 to 120.4%. Coefficients of variation for intra- and interassay variability of 3 serum samples ranged from 7.6 to 11.9% and from 10.1 to 13.1%, respectively. Mean +/- SD serum concentration of cPG A in healthy dogs was 63.8 +/- 31.0 microg/L and the reference range was 18 to 129 microg/L. Significant increases in serum concentrations of cPG A were observed between 1 and 7 hours after feeding.
  
  
  CONCLUSIONS
  The ELISA for measuring cPG A was sufficiently sensitive, linear, accurate, precise, and reproducible for clinical use. Serum concentrations of cPG A increase substantially after feeding, which should be taken into account when conducting clinical studies.
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis; D004285:Dogs / Q000097:blood*; D004435:Eating / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D005756:Gastritis / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary; D008297:Male; D020116:Pepsinogen A / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D020116:Pepsinogen A