Determination of melagatran in rabbit plasma by high-performance liquid chromatography with automated column switching.
Melagatran is an active thrombin inhibitor showing oral and parenteral bioavailability for antithrombotic therapy. A simple and convenient liquid chromatographic method has been developed and applied to the analysis of melagatran in rabbit plasma. The clean-up and separation of the sample solutions were performed by automated on-line column switching HPLC. The method validation shows the suitability of the column switching liquid chromatographic system for the quantitation of melagatran in biological fluids.
D000818:Animals; D001331:Automation; D001384:Azetidines; D001596:Benzylamines; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood*; D011817:Rabbits; D015203:Reproducibility of Results
D001384:Azetidines; D001596:Benzylamines; C109573:melagatran; D005998:Glycine