title: Stabilisation of eudesmane cation by tryptophan 334 during aristolochene synthase catalysis.
abstract: Analysis of the hydrocarbons produced during catalysis by mutants of aristolochene synthase from Penicillium roqueforti indicated that Trp 334 had a pivotal function for the efficient production of aristolochene from farnesylpyrophosphate most likely by stabilising the intermediate, eudesmane cation.
mesh_terms: D002384:Catalysis; D002412:Cations; D007535:Isomerases / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry*; D045787:Sesquiterpenes, Eudesmane; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D002412:Cations; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; C054277:aristolochene; C015872:eudesmane; C004808:farnesyl pyrophosphate; D014364:Tryptophan; D007535:Isomerases; C060050:aristolochene synthase