Preparation of peptide-loaded dendritic cells for cancer immunotherapy.
Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood progenitors, particularly plastic adherent or CD14+ monocytes, in media supplemented with GM-CSF and IL-4. These DC may then be matured, if desired, and loaded with antigen, such as tumor-associated peptides, prior to administration. We describe the scheme that we are currently using to generate peptide-loaded dendritic cells for our clinical trials of cancer immunotherapy.
D023201:CD40 Ligand / Q000494:pharmacology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification; D002272:Carcinoembryonic Antigen / Q000737:chemistry / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D002454:Cell Differentiation / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015788:HLA-A1 Antigen / Q000276:immunology; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D016233:Immunotherapy, Active / Q000379:methods; D007371:Interferon-gamma / Q000494:pharmacology; D007937:Leukapheresis / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D010455:Peptides / Q000276:immunology*; D048008:TNF Receptor-Associated Factor 3; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D002352:Carrier Proteins; D015788:HLA-A1 Antigen; D010455:Peptides; D048008:TNF Receptor-Associated Factor 3; C090656:TRAF3 protein, human; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D014409:Tumor Necrosis Factor-alpha; D023201:CD40 Ligand; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor