Immunomagnetic separation and detection of Salmonella cells using newly designed carriers.
Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and polymerase chain reaction (PCR) methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells.
D004587:Electrophoresis, Agar Gel; D018189:Immunomagnetic Separation; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000166:cytology*
D008689:Methacrylates; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate