The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group.
Injection of rabbits with Freund's complete adjuvant containing bovine gamma-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen).
D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies; D000941:Antigens; D002417:Cattle; D005620:Freund's Adjuvant; D006241:Haptens; D009596:Nitrophenols; D011817:Rabbits; D005719:gamma-Globulins
D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; D006241:Haptens; D009596:Nitrophenols; D005719:gamma-Globulins; D005620:Freund's Adjuvant
ANTIBODIES; NITROPHENOLS