Effects of 6-aminonicotinamide gliotoxin on blood-brain barrier differentiation in the chick embryo cerebellum.
The hypothesis of astroglial cell involvement in prenatal setting up of the blood-brain barrier (BBB) has been examined by producing glial degeneration in cerebellum of chicken embryos submitted to the action of gliotoxin 6-aminonicotinamide (6-AN), which was applied onto the embryo chorioallantoic membrane during both early and late embryonic development. The effects of 6-AN on the cerebellum astroglial cells and microvessels were analysed under the light microscope by immunostaining for 3CB2 (chick-specific glial marker) and HT7 (chick-specific marker of BBB-provided brain endothelia), under the electron microscope, as well as by the vascular permeability tracer horseradish peroxidase. The results, showing good suitability of the 6-AN model also when applied in early embryonic development, demonstrated a correlation between perivascular glia depletion and endothelial barrier impairment and suggested that astroglia play a role in the BBB prenatal differentiation.
D015120:6-Aminonicotinamide / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D030161:Avian Proteins; D051926:Basigin; D001798:Blood Proteins; D001812:Blood-Brain Barrier / Q000166:cytology / Q000187:drug effects / Q000196:embryology* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002531:Cerebellum / Q000098:blood supply / Q000187:drug effects / Q000196:embryology*; D002642:Chick Embryo; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology; D006735:Horseradish Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis; D008833:Microcirculation / Q000196:embryology* / Q000648:ultrastructure; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D008562:Membrane Glycoproteins; D051926:Basigin; D015120:6-Aminonicotinamide; D006735:Horseradish Peroxidase