title: Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine.
abstract: Organophosphate (OP) pesticides are widely used as antiparasitic chemicals in finfish aquaculture. However, current antidotes cannot be applied to treat intoxicated fish. We showed in previous studies the importance of glutathione (GSH) metabolism in pesticide resistance of the European eel Anguilla anguilla L. The present work studied the effects of the antioxidant and glutathione pro-drug N-acetyl-L-cysteine (NAC) on the recovery of European eels exposed for 96 h to a sublethal concentration (0.17 mg l(-1); 20% of its 96 h LC50) of the OP pesticide dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP). This insecticide and acaricide decreased muscular GSH content and increased oxidised glutathione (GSSG), lowering the GSH:GSSG ratio, which is indicative of a condition of oxidative stress. Acetylcholinesterase (AChE) and glutathione reductase (GR) activities in the brain, which were biomarkers of neurotoxicity and oxidative stress, respectively, were also highly inhibited. Recovery in a 0.5 mM (81.6 mg l(-1)) NAC concentration ameliorated muscular GSH depletion, GSH:GSSG ratio, and the inhibition of brain AChE and GR activities. Hence, this is the first evidence of improved recovery of organophosphate-poisoned fish by bath treatments.
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000704:Analysis of Variance; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001494:Baths; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002800:Cholinesterase Inhibitors / Q000493:pharmacokinetics / Q000633:toxicity; D004006:Dichlorvos / Q000493:pharmacokinetics* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000187:drug effects; D008658:Inactivation, Metabolic; D007306:Insecticides / Q000493:pharmacokinetics* / Q000633:toxicity; D011355:Prodrugs / Q000008:administration & dosage / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D011355:Prodrugs; D014874:Water Pollutants, Chemical; D004006:Dichlorvos; D005980:Glutathione Reductase; D000110:Acetylcholinesterase; D005978:Glutathione; D000111:Acetylcysteine