Antioxidant effect of flavonoids on DCF production in HL-60 cells.
A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated.
D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D005452:Fluoresceins / Q000737:chemistry; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D010084:Oxidation-Reduction; D008517:Phytotherapy; D010946:Plants, Medicinal; D016897:Respiratory Burst / Q000187:drug effects*
D000975:Antioxidants; D005419:Flavonoids; D005452:Fluoresceins; C029569:diacetyldichlorofluorescein