Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries.
Cytotoxic T-cells are the most important effector cells in immune responses against tumors. The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines. Here, we describe a new approach to the determination of tumor-associated T-cell epitopes which employs combinatorial peptide libraries with singly defined sequence positions in a randomized context. The analysis of the responses of a T-cell clone to these libraries yields the amino acid constituents of the epitope which can be combined to obtain mimotopes that are suitable as vaccine antigens for the induction of tumor-specific responses.
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D020126:Brefeldin A / Q000494:pharmacology; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D030562:Databases, Protein; D015195:Drug Design; D018604:Epitope Mapping / Q000379:methods; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D015448:Leukemia, B-Cell / Q000276:immunology / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology; D015166:Monitoring, Immunologic; D019151:Peptide Library; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D019151:Peptide Library; D020126:Brefeldin A; C016658:phorbolol myristate acetate; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate