title: Effects of oestrogen deficiency on rat mandibular and tibial microarchitecture.
abstract: OBJECTIVE
  To investigate the effects of oestrogen deficiency on the microarchitecture of trabecular bone in the mandible and the tibia and to test whether they are correlated.
  
  
  METHODS
  Twenty-four age-matched Lewis-Brown-Norway female rats underwent surgical intervention either to remove ovaries (ovariectomy, n=12) or to create a complementary control group (sham-operated, n=12). Sixteen weeks later, the animals were sacrificed and the left side of the mandibles and the tibias were scanned with high resolution micro-CT (15 micro m). Multiple morphological measures including the ratio of bone volume/tissue volume, trabecular thickness, trabecular separation and structure model index were obtained from the experimental and control groups.
  
  
  RESULTS
  Ovariectomy significantly decreased the ratio of bone volume/soft tissue volume and trabecular thickness, whilst significantly increasing trabecular separation and structure model index in the mandible (P<0.005) and the tibia (P<0.005). There were significant positive correlations between the mandible and the tibia for trabecular separation (r=0.68, P<0.01) and structure model index (r=0.60, P<0.01).
  
  
  CONCLUSIONS
  Oestrogen deficiency results in microarchitectural alterations of trabecular bone in both the mandible and the tibia within 16 weeks. The size of marrow spaces and the shape of trabeculae in the mandible correlate with osteoporotic changes in the long bone.
mesh_terms: D000818:Animals; D015519:Bone Density; D001853:Bone Marrow / Q000000981:diagnostic imaging / Q000648:ultrastructure; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D006132:Growth Plate / Q000000981:diagnostic imaging / Q000648:ultrastructure; D008334:Mandible / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000473:pathology; D010052:Ovariectomy; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D018709:Statistics, Nonparametric; D013977:Tibia / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D004967:Estrogens