[Necrolytic properties of alkaline protease in the treatment of severe eye burns].
Both eyes of 62 rabbits (124 eyes) were experimentally studied. Necrolytic properties of alkaline protease were examined on a model of an isolated alkaline retinal burn by 10% NaOH with a 10 sec exposure; the protease optimal concentration and an optimal exposure were determined. It was established that a necrolytic effect correlating with that of lecozyme can be achieved when a protease solution with an activity of 25-27 PE is used. An increased activity of protease in the solution results not only in hydrolysis of the turbid corneal part, but it also melts the perifocal area entailing a danger of perforation. Alkaline protease can be used as an ointment to ensure the necrolytic effect, however, its dosage in such medication form must be above 30-35 PE. The administration of a preliminary enzymatic necrectomy produced a positive effect on the postoperative cause and the results of keratoplasty as compared with surgical necrectomy, which can be explained by a better quality necrectomy involving the use of alkaline protease.
D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy* / Q000473:pathology; D002424:Caustics; D016039:Corneal Transplantation; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000008:administration & dosage / Q000627:therapeutic use*; D005126:Eye Burns / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D006868:Hydrolysis; D009336:Necrosis; D009824:Ointments; D011817:Rabbits; D012972:Sodium Hydroxide; D012996:Solutions
D002424:Caustics; D009824:Ointments; D012996:Solutions; D012972:Sodium Hydroxide; D010450:Endopeptidases
Nekroliticheskie svoĭstva shchelochnoĭ proteazy v lechenii ozhogov glaz tiazhelykh stepeneĭ.