title: Characterization of a new densovirus infecting the green peach aphid Myzus persicae.
abstract: A new icosahedral DNA virus was isolated from aphids (Myzus persicae) that showed abnormal growth and development. The purified virus particles have a diameter of 20 nm and contain a single-stranded DNA molecule of approximately 5.7 kb. The viral particles are composed of five structural proteins (92, 85, 68, 64, and 57 kDa). As the main biophysical properties of this virus are similar to those of the members of the genus Densovirus it was tentatively named Myzus persicae densovirus (MpDNV). A PCR-based detection method and a polyclonal antiserum raised against MpDNV allowed the detection of the virus in a single-infected aphid. MpDNV is immunologically related to Junonia coenia densovirus, but not to other members of the subfamily Densovirinae. Biological assays showed that MpDNV could be both transmitted transovarially and horizontally via honeydew and saliva. MpDNV was able to infect whiteflies but not other aphid species tested.
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D015153:Blotting, Western; D017990:Densovirus / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D018562:Disease Transmission, Infectious; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D018445:Infectious Disease Transmission, Vertical; D008854:Microscopy, Electron; D010322:Parvoviridae Infections / Q000635:transmission*; D016133:Polymerase Chain Reaction