Oleanolic glycosides from Pometia ridleyi.
Six triterpenoid saponins were isolated from the stem bark of Pometia ridleyi along with two known saponins, acutoside A and calenduloside C. Their structures were established using one- and two-dimensional NMR and mass spectrometry as 3-O-beta-D-apiofuranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-beta-D-glucopyranosyl-, 3-O-beta-D-apiofuranosyl-(1-->3)-alpha-L-arabinopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-beta-D-glucopyranosyl-, 3-O-beta-D-apiofuranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-beta-D-glucopyranosyl-, 3-O-alpha-L-arabinopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyl-, 3-O-beta-D-galactopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyl-, 3-O-beta-D-apiofuranosyl-(1-->3)-beta-D-galactopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyl-oleanolic acid. The EtOH and EtOAc extracts of the stem bark showed no cytotoxic activity. At a concentration of 23 microg/ml, the saponin mixture showed haemolytic activity and caused 50% haemolysis of a 10% suspension of sheep erythrocytes.
D000818:Animals; D002236:Carbohydrate Conformation; D004912:Erythrocytes / Q000187:drug effects; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D029631:Sapindaceae / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012756:Sheep; D021241:Spectrometry, Mass, Electrospray Ionization
D006027:Glycosides; D012503:Saponins; D009828:Oleanolic Acid