Practical use of DD-peptidase 64-575 for the assay of inhibition activity of natural and synthetic beta-lactam compounds.
DD-peptidase 64-575 is produced by Saccharopolyspora erythrea PZH TZ 64-575. This enzyme is very sensitive to beta-lactam antibiotics. It could be used to assay the affinity of natural and synthetic beta-lactam compounds. In this paper an enzymatic method is described for investigation of synthetic, insoluble in water beta-lactams.
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D047090:beta-Lactams; D002268:Carboxypeptidases; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase