The fine structure of Streptomyces coelicolor. II. The nuclear material.
Colonies and spore suspensions of Streptomyces coelicolor were fixed for electron microscopy by the method of Kellenberger, Ryter, and Séchaud (1958). In thin sections the nuclear regions have a lower average density than the cytoplasm and the outlines of these regions correspond well with the profiles of the chromatinic bodies observed with the light microscope. The nuclear regions contain fibrils, about 5 mmicro in diameter. In contrast, after fixation by the method of Palade (1952) the nuclear material is coagulated into irregular dense masses and tubular structures about 20 mmicro in diameter, lying in a nuclear "vacuole." The significance of these observations is discussed in relation to the observations of other workers on the fine structure of the nuclear material of other bacteria and the chromosomes of higher cells.
D001419:Bacteria; D002843:Chromatin; D003029:Coagulants; D003593:Cytoplasm; D006652:Histological Techniques; D008854:Microscopy, Electron; D013302:Streptomyces; D048372:Streptomyces coelicolor; D014617:Vacuoles
D002843:Chromatin; D003029:Coagulants
STREPTOMYCES