Cat heart muscle in vitro. I. Cell volumes and intracellular concentrations in papillary muscle.
Methods have been developed for the simultaneous determination of total water, inulin space, and K and Na content in muscles of 0.5 to 10 mg. wet weight. These methods have been used to define steady state conditions with respect to intracellular K concentration in papillary muscles from cat hearts perfused and contracting isometrically at 27-28 degrees C. and at 37-38 degrees C. Cell volumes and intracellular ionic concentrations have been followed as a function of the external K concentration and compared with values predicted on the basis of electroneutrality and osmotic equilibrium.
D000818:Animals; D002415:Cats; D048429:Cell Size; D003593:Cytoplasm; D066298:In Vitro Techniques; D007477:Ions; D009132:Muscles; D009206:Myocardium / Q000737:chemistry*; D010210:Papillary Muscles; D012964:Sodium; D014867:Water
D007477:Ions; D014867:Water; D012964:Sodium
MYOCARDIUM/chemistry