Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.
The lysis of Group A type 1 streptococcal cell walls by phage-associated lysin has been described. In the preparation of lysin, a new semisynthetic non-protein media to support growth of the propagating strain of Group C streptococci was employed. Following lysis of the cell walls, the resulting digest was partially purified by ion-exchange chromatography and ammonium sulfate precipitation. In addition to M protein, the resulting preparation (called lysin M protein) contained the group-specific carbohydrate and the T protein-but did not contain antigens, detectable by precipitin tests, which cross-reacted with absorbed heterologous group or type antisera. Capillary precipitin reactions between the lysin M protein and type-specific antiserum did not occur in the presence of high ionic strength buffers; these buffers did not similarly affect precipitin reactions of acid M protein. Type 1 lysin M protein is shown to be a good antigen. A total of 1.5 mg. injected intradermally in saline produced bactericidal antibody in eight of nine rabbits; when injected in adjuvant in one rabbit, protective serum antibodies developed. Streptococci grown in sera from seven of nine rabbits immunized with lysin M protein demonstrated significantly longer chains than when grown in normal rabbit serum. Antibody as demonstrated by each of these three tests was shown to be type-specific. No local or systemic toxicity was noted following intradermal injection in rabbits of lysin M protein.
D000818:Animals; D000906:Antibodies; D000941:Antigens; D001435:Bacteriophages; D002473:Cell Wall; D003429:Cross Reactions; D007106:Immune Sera; D009088:Mucoproteins; D009194:Myeloma Proteins; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D013291:Streptococcus / Q000378:metabolism*; D013297:Streptococcus pyogenes
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D009088:Mucoproteins; D009194:Myeloma Proteins; D011506:Proteins; C010393:lysin, gastropoda; C085016:multiple myeloma M-proteins
ANTIBODIES; PROTEINS/metabolism; STREPTOCOCCUS/metabolism