Fine structures of intracytoplasmic organelles of mycobacteria.
The fine structure of the intracytoplasmic organelles of mycobacteria was studied by means of electron microscopy of ultrathin sections. A well-preserved nuclear apparatus was obtained by fixation with OsO(4) in acetate-veronal buffer, containing calcium and tryptone, or in collidine-HCl buffer, followed by uranyl-acetate treatment and embedding in araldite. A low density nuclear region was filled with fine fibrils, 30 A in diameter, in parallel or concentric arrangement. A membranous organelle, tentatively designated as "lamellar structure," consists of unit membranes in lamellar arrangement. The thickness of each lamella in this membranous organelle coincides with that of the three-layered cytoplasmic membrane Moreover, the continuity of this unit membrane with the cytoplasmic membrane was demonstrated.
D002462:Cell Membrane; D004853:Epoxy Resins; D005109:Extracellular Matrix; D006652:Histological Techniques; D008854:Microscopy, Electron; D009161:Mycobacterium / Q000033:anatomy & histology*; D015388:Organelles; D009942:Organometallic Compounds; D010796:Phthalic Anhydrides; D011309:Preservation, Biological; D011725:Pyridines; D012106:Research
D004853:Epoxy Resins; D009942:Organometallic Compounds; D010796:Phthalic Anhydrides; D011725:Pyridines; C005460:uranyl acetate; C005752:araldite; C007106:gamma-collidine
MYCOBACTERIUM/anatomy and histology