The direct isolation of the mitotic apparatus.
A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.
D000818:Animals; D002455:Cell Division; D010063:Ovum; D012617:Sea Urchins; D008941:Spindle Apparatus; D013395:Sucrose
D013395:Sucrose
CELL DIVISION