Biochemical study of cellular antigen-antibody reaction in tissue culture. II. Release of a protease inhibitor.
Decrease in the protease activity of the culture fluid observed at later stages of the antigen-antibody reaction is believed to be due to the release of an inhibitor by the cells. The inhibitor was submitted to partial purification: it is heat-stable, non-precipitated by trichloracetic acid and non-dialyzable. It inhibits certain cellular and tissue proteases and papain but is inactive against trypsin. It is suggested that the balance between protease and anti-protease released may determine the intensity, extent, and duration of certain sensitization phenomena.
D000932:Antiemetics / Q000628:therapy*; D000937:Antigen-Antibody Reactions; D010450:Endopeptidases; D010447:Peptide Hydrolases; D011480:Protease Inhibitors; D046509:Tissue Culture Techniques / Q000378:metabolism*; D014357:Trypsin
D000932:Antiemetics; D011480:Protease Inhibitors; D010450:Endopeptidases; D010447:Peptide Hydrolases; D014357:Trypsin
ANTIEMETICS/therapy; PROTEASES/antagonists; TISSUE CULTURE/metabolism