Preparation of monolayer cell cultures from tissues of some lower vertebrates.
Cold trypsin dispersion at pH 7.2 was used to obtain cultivable cells and cell groups from tissues of six species of fresh-water bony fishes, a frog, and a turtle. The cells readily attached to glass and were capable of at least limfted, and in some cases extended, division in media consisting of commercially available components.
D000663:Amphibians; D000818:Animals; D005399:Fishes; D046509:Tissue Culture Techniques; D014714:Vertebrates
AMPHIBIA; FISH; TISSUE CULTURE