Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.
Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing toxin on a diethylaminoethyl cellulose column and eluting it with a gradient concentration of NaCl. Toxin preparations thus obtained consisted mainly of protein; their toxicity was destroyed by trypsin but not by ribonuclease or deoxyribonuclease.
D000941:Antigens; D000992:Antitoxins; D001886:Bordetella pertussis / Q000276:immunology*; D006358:Hot Temperature; D014118:Toxins, Biological
D000941:Antigens; D000992:Antitoxins; D014118:Toxins, Biological
ANTIGENS; BORDETELLA PERTUSSIS/immunology; TOXINS AND ANTITOXINS