Antigenic properties of purified fractions from Ascaris lumbricoides var. Suum on naturally sensitized guinea-pig.
Purified extract of Ascaris injected intravenously into guinea-pigs produced a reaction very similar to the anaphylactic reaction. As shown by the ratio of deaths to survivals the toxic fraction contained in Ascaris extract was purified 3 to 4 times. After intravenous injection animals presented both anaphylaxis-like shock and mast cell damage similar to that observed in anaphylaxis. Perfusion of the lungs with Ascaris fractions released histamine. Isolated guinea-pig ileum when in contact with Ascaris fractions showed a response followed by desensitization. Heating the ileum to 45 degrees C or previous desensitization to anaphylatoxin excluded the participation of the latter in this contraction. In vitro, sensitization from one guinea-pig could be transferred to another, using spleen extract or a 50% ammonium sulphate precipitate obtained from the spleen extract. On the basis of these results it is concluded that guinea-pigs are naturally sensitized to antigen or antigens present in Ascaris lumbricoides.
D000707:Anaphylaxis; D000818:Animals; D000941:Antigens; D001196:Ascariasis; D001200:Ascaris; D017164:Ascaris lumbricoides; D003888:Desensitization, Immunologic; D006168:Guinea Pigs; D006632:Histamine; D007082:Ileum; D008168:Lung
D000941:Antigens; D006632:Histamine
ANTIGENS; ASCARIS/extracts