The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes.
An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. Antibody-antigen mixtures when incubated (37 degrees C) in medium containing fresh (i.e. non-inactivated) normal rabbit serum exert a strong chemotactic effect on rabbit polymorphonuclear leucocytes. Results are described which indicate that, when antibody-antigen complexes are incubated (37 degrees C) in fresh serum, a heat-stable (56 degrees C) substance (or substances) is produced which acts directly as a chemotactic stimulus on the polymorphs. This heat-stable chemotactic substance is not produced when antibody-antigen complexes are incubated in serum which has been heated at 56 degrees C for 30 minutes.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007962:Leukocytes / Q000737:chemistry*; D009504:Neutrophils; D011817:Rabbits
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens
ANTIBODIES; ANTIGENS; LEUKOCYTES/chemistry