Effect of L-tryptophan on diuresis and 5-hydroxyindoleacetic acid excretion in the rat.
Oral administration of L-tryptophan to rats produced two main biochemical and pharmacological effects: a marked increase in urinary 5-hydroxyindoleacetic acid excretion, and a significant reduction in the urine flow after a water load. Urinary 5-hydroxyindoleacetic acid excretion reached its maximum 2 to 6 hr after the administration of tryptophan, and it increased with the dose of the amino acid. Antidiuresis was seen after the administration of L-tryptophan, 200 mg/kg, or more. The effect appeared promptly and it was roughly proportional to the dose of the amino acid administered. Both antidiuretic effect and increase in urinary 5-hydroxyindoleacetic acid excretion were more intense after oral than after parenteral administration of L-tryptophan. D-Tryptophan, in oral doses up to 1,000 mg/kg, produced neither an increase in urinary 5-hydroxyindoleacetic acid nor a reduction of diuresis. Available evidence suggests that reduction of urine flow is a consequence of biosynthesis and release of 5-hydroxytryptamine by the gastrointestinal mucosa. Tryptamine produced by direct decarboxylation of L-tryptophan does not seem to play any important role.
D000284:Administration, Oral; D000596:Amino Acids; D000818:Animals; D004231:Diuresis; D006897:Hydroxyindoleacetic Acid; D007210:Indoleacetic Acids / Q000031:analogs & derivatives; D007668:Kidney / Q000494:pharmacology*; D008297:Male; D051381:Rats; D012701:Serotonin; D014363:Tryptamines; D014364:Tryptophan / Q000494:pharmacology*
D000596:Amino Acids; D007210:Indoleacetic Acids; D014363:Tryptamines; D012701:Serotonin; C030820:tryptamine; D006897:Hydroxyindoleacetic Acid; C030737:indoleacetic acid; D014364:Tryptophan
INDOLACETIC ACID/related compounds; KIDNEY/pharmacology; TRYPTOPHAN/pharmacology