Contribution of E-amino groups to ninhydrin color production in proteins.
The ninhydrin color given by model compounds and by characterized proteins shows a consistent contribution from the epsilon-amino group of lysine of about 67 percent of that of an alpha-amino group, except in free lysine or in N-terminal lysine, where the epsilon-amino group makes a small contribution (7 to 10 percent) to the total color. This information can be applied to structure determination of N(epsilon) peptides of lysine.
E
E
SLOBODIAN
G
G
MECHANIC
M
M
LEVY
D008239:Lysine / Q000737:chemistry*; D009555:Ninhydrin; D010455:Peptides; D011506:Proteins / Q000737:chemistry*
D010455:Peptides; D011506:Proteins; D009555:Ninhydrin; D008239:Lysine
LYSINE/chemistry; PROTEINS/chemistry