A new epoxy embedment for electron microscopy.
A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Epon-812-embedded tissues, Maraglas-Cardolite-embedded tissues can be sectioned in large dimensions with ease and consistent results without "chatter." No background granularity is detectable with high magnification study of Maraglas-Cardolite-embedded tissues. This epoxy is readily stained with lead hydroxide and is relatively stable in the electron beam.
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FREEMAN
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SPURLOCK
D004583:Electrons; D004853:Epoxy Resins; D008853:Microscopy; D008854:Microscopy, Electron; D008867:Microtomy; D012117:Resins, Synthetic
D004853:Epoxy Resins; D012117:Resins, Synthetic; C004875:EPON
MICROSCOPY, ELECTRON; MICROTOMY; RESINS