An immunologic study of two metabolically active peptides from the anterior pituitary gland.
Peptides I and II, two metabolically active peptides from porcine pituitary glands, stimulated antibody production in rabbits. The antiserum obtained had antibodies which interacted with peptides I and II from porcine pituitaries, peptides I and II from human pituitaries, and fraction H prepared by Rudman from hog pituitaries. None of the other pituitary preparations tested showed any interaction with peptides I and II. All three preparations stimulated the release of free fatty acids from rabbit adipose tissue in vitro. Antiserum inhibited this biological action to a different extent in each of the three preparations. Following an intravenous injection of peptides I and II there was a rapid rise in the plasma concentration of free fatty acids in rabbits. Injection of antiserum together with peptides I and II failed to block the rapid increase in free fatty acids. However, an injection of at least ten times the minimal amount of peptides I and II which raised the plasma free fatty acid concentration produced no increase in a rabbit with a high antibody titer to these peptides.
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BARRETT
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified; D006801:Humans; D050356:Lipid Metabolism; D010455:Peptides / Q000737:chemistry*; D010902:Pituitary Gland; D010903:Pituitary Gland, Anterior / Q000737:chemistry*; D011817:Rabbits; D000075370:Receptors for Activated C Kinase; D013552:Swine
D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D010455:Peptides; D000075370:Receptors for Activated C Kinase; C070301:peptide I
ADIPOSE TISSUE/metabolism; ANTIBIOTICS/metabolism; FATTY ACIDS/metabolism; PEPTIDES/chemistry; PITUITARY GLAND, ANTERIOR/chemistry