Glycolate metabolism in Escherichia coli.
Hansen, Robert W. (University of Illinois College of Medicine, Chicago) and James A. Hayashi. Glycolate metabolism in Escherichia coli. J. Bacteriol. 83:679-687. 1962.-This study of glycolate-adapted Escherichia coli indicates that the most probable route for utilization of the substrate includes glyceric acid, 3-phosphoglyceric acid, and the tricarboxylic acid cycle. A glyceric acid dehydrogenase, which reduces tartronic semialdehyde to glycerate in the presence of reduced diphosphopyridine nucleotide, and a kinase, which catalyzes the formation of 3-phosphoglycerate from glyceric acid and adenosine triphosphate, were shown to be present. Carbon recoveries in growing cultures and manometric data obtained with resting cells showed the complete oxidation of glycolate to carbon dioxide. Measurements of the oxidation of tricarboxylic acid cycle intermediates indicated that these compounds are oxidized without lag and at a rate commensurate with the rate of glycolate oxidation. Assays of the enzymes characteristic of known pathways of terminal oxidation, such as isocitratase, malate synthetase, isocitric dehydrogenase, and condensing enzyme, provided further evidence for an operating tricarboxylic acid cycle. A postulated pathway for the utilization of glycolic acid is as follows: glycolate --> glycerate --> 3-phosphoglycerate --> pyruvate --> tricarboxylic acid cycle.
HANSEN
R W
RW
HAYASHI
J A
JA
D000085:Acetates / Q000378:metabolism*; D002950:Citrate (si)-Synthase; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000378:metabolism*; D005988:Glyceric Acids; D006016:Glycolates; D009243:NAD; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D010770:Phosphotransferases
D000085:Acetates; D005988:Glyceric Acids; D006016:Glycolates; D009243:NAD; C031149:glycolic acid; C005156:3-phosphoglycerate; D010088:Oxidoreductases; D002950:Citrate (si)-Synthase; D010770:Phosphotransferases
ACETATES/metabolism; ESCHERICHIA COLI/metabolism