Fractionation of beta-glucosidases and related extracellular enzymes from Aspergillus niger.
Industrial concentrates from Aspergillus niger culture filtrates were fractionated by ion-exchange and adsorption chromatography. Several other types of hydrolases were completely removed. Eight partially purified components were obtained. Using specific activity as an estimate of purification, one aryl-beta-glucosidase was purified 35-fold. Another component showed 147-fold purification using a viscosimetric assay with carboxymethylcellulose as substrate. The aryl-beta-glucosidase was distinctly more thermolabile than the carboxymethylcellulase.
D001230:Aspergillus; D001234:Aspergillus niger; D002480:Cellulase; D044602:Cellulases; D005959:Glucosidases; D001617:beta-Glucosidase
D044602:Cellulases; D005959:Glucosidases; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase
ASPERGILLUS; GLUCOSIDASE