Eliminative split of pectic substances by phytopathogenic soft-rot bacteria.
Culture filtrates of Erwinia carotovora and Bacillus polymyxa split pectic substances to yield a product that absorbs at 230 mmicro, and which reacts with thiobarbituric acid to form a substance that absorbs at 547 mmicro. This product is believed to be a C-4,5-unsaturated oli-gouronide. The preferred substrate is polygalacturonic acid rather than pectin; the enzyme, provisionally named polygalacturonic-trans-eliminase, is activated by calcium.
D001407:Bacillus; D001419:Bacteria / Q000378:metabolism*; D002118:Calcium; D050260:Carbohydrate Metabolism; D004798:Enzymes / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism*; D014574:Uronic Acids / Q000378:metabolism*
D004798:Enzymes; D014574:Uronic Acids; D010368:Pectins; D002118:Calcium; C003181:polygalacturonic acid
BACTERIA/metabolism; ENZYMES/metabolism; PECTINS/metabolism; URONIC ACIDS/metabolism