Continuous culture of ruminal microorganisms in chemically defined medium. I. Design of continuous-culture apparatus.
An apparatus is described which has been used for successful continuous culture of the ciliates from the rumen of cattle. Automatic control of feeding rate, pH, oxidation-reduction potential, temperature, stirring rate, aeration rate, salinity, and volume of the culture is provided for, using standard commercial equipment, whenever possible. The operation of this apparatus is described.
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D016798:Ciliophora; D048388:Gadiformes; D012417:Rumen
BACTERIOLOGICAL TECHNICS; CILIATA; RUMEN