Nutrition of five Bacteroides strains.
Quinto, Grace (University of Kentucky College of Medicine, Lexington). Nutrition of five Bacteroides strains. J. Bacteriol. 84:559-562. 1962.-Some of the nutritional requirements of five gram-negative anaerobic bacilli, including Ristella perfoetens, Zuberella clostridiformis, and three Bacteroides strains freshly isolated from clinical exudates, were investigated. A fluid maintenance medium was developed in which the three freshly isolated strains, C-4, C-7, and C-2795, grew maximally in 12 to 24 hr. The maintenance medium contained 2.0% Trypticase and Proteose Peptone, 0.5% glucose, and 0.1% sodium thioglycolate; it was adjusted to pH 7.2 and supplemented with 0.1 mug of hemin/ml. Strains C-4, C-7, and C-2795 were cultivated through 14 serial cultures in fluid maintenance medium containing 0.1 mug of hemin/ml. The most satisfactory inoculum was a 1:100 or 1:1,000 dilution of a 24-hr seed culture. All the strains except Z. clostridiformis grew serially in a defined medium. R. perfoetens required pantothenic acid, nicotinic acid, and the following amino acids: histidine, tryptophan, tyrosine, valine, phenylalanine, cystine, and probably arginine, glutamic acid, methionine, glycine, isoleucine, leucine, and lysine. The C-4, C-7, and C-2795 strains required the hemin supplement in defined medium, but not vitamins, purines, or pyrimidines.
D000596:Amino Acids; D001120:Arginine; D001439:Bacteroides; D003470:Culture Media; D005673:Fusobacterium; D005947:Glucose; D018698:Glutamic Acid; D006639:Histidine; D008239:Lysine; D010649:Phenylalanine; D011687:Purines; D014443:Tyrosine; D014815:Vitamins
D000596:Amino Acids; D003470:Culture Media; D011687:Purines; D014815:Vitamins; D018698:Glutamic Acid; D014443:Tyrosine; D010649:Phenylalanine; D006639:Histidine; D001120:Arginine; D005947:Glucose; D008239:Lysine; C030985:purine
BACTEROIDES; CULTURE MEDIA