Lipid metabolism of rumen ciliates and bacteria. I. Uptake of fatty acids by Isotricha prostoma and Entodinium simplex.
Washed suspensions of the ruminal ciliates, Isotricha prostoma and Entodinium simplex, concentrated C(14)-labeled oleic, palmitic, stearic, and linoleic acids within the cells during short incubation periods. Radioautographs demonstrated that oleic acid-1-C(14) was hydrogenated to stearic acid by I. prostoma, and Warburg manometric data showed that the sodium salts of oleic, valeric, caproic, and acetic acids, and methyl myristate, methyl laurate, and the triglyceride tributyrin stimulated fermentation of I. prostoma. The total lipid and free fatty acid contents of I. prostoma were determined.
D000818:Animals; D001419:Bacteria; D016798:Ciliophora; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005285:Fermentation; D048388:Gadiformes; D006865:Hydrogenation; D008041:Linoleic Acids; D050356:Lipid Metabolism; D009829:Oleic Acids; D012417:Rumen; D013229:Stearic Acids; D016800:Trichostomatida
D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D009829:Oleic Acids; D013229:Stearic Acids; C031183:stearic acid
CILIATA; LIPID METABOLISM; RUMEN