The isolation of nuclei from eggs and embryos of the sea urchin.
NUCLEI CAN BE ISOLATED FROM THE EGGS AND EMBRYOS OF THE SEA URCHIN BY A FOUR STEP METHOD: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used.
D000818:Animals; D002467:Cell Nucleus; D004447:Echinodermata; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D010063:Ovum; D012617:Sea Urchins
CELL NUCLEUS; ECHINODERMATA; EMBRYO; OVUM