Albumin replacement by fatty acids in clonal growth of mammalian cells.
Clonal growth of Chinese hamster strain CHD-3 cells in a synthetic nutrient mixture supplemented with 10 micrograms of purified fetuin per milliliter exhibits an apparent requirement for serum albumin. The albumin can be replaced by linoleic acid, which occurs as a tightly bound component of most albumin preparations. Linolenic acid and corn oil can also replace albumin, while oleic acid and esters of linoleic and linolenic acids cannot.
D000418:Albumins; D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids; D019301:Oleic Acid; D009829:Oleic Acids; D012709:Serum Albumin; D046509:Tissue Culture Techniques
D000418:Albumins; D005227:Fatty Acids; D009829:Oleic Acids; D012709:Serum Albumin; D019301:Oleic Acid
FATTY ACIDS; SERUM ALBUMIN; TISSUE CULTURE