Comparisons of Bence-Jones proteins and L polypeptide chains of myeloma globulins after hydrolysis with trypsin.
L polypeptide chains of myeloma globulin and Bence-Jones protein isolated from the same patient were found to be identical after comparison of their tryptic hydrolysates by two-dimensional high voltage electrophoresis. The patterns of peptides from proteins belonging to antigenic group I differed markedly from those of proteins in antigenic group II. A partially purified H chain fraction was compared with L chains from the same myeloma protein. The tryptic hydrolysates yielded dissimilar patterns of peptides. These data indicate that gamma-myeloma proteins contain two kinds of polypeptide chains, Hgamma chains and either L(I) or L(II) chains. The L chains appear to be identical with those comprising the Bence-Jones protein from the same patient.
D000941:Antigens; D001536:Bence Jones Protein; D006801:Humans; D006868:Hydrolysis; D009101:Multiple Myeloma; D010455:Peptides; D012712:Serum Globulins; D014357:Trypsin; D005719:gamma-Globulins
D000941:Antigens; D010455:Peptides; D012712:Serum Globulins; D005719:gamma-Globulins; D001536:Bence Jones Protein; D014357:Trypsin
MULTIPLE MYELOMA; PEPTIDES; SERUM GLOBULIN; TRYPSIN