Glutamate metabolism in Brucella abortus strains of low and high virulence.
Dasinger, B. L. (University of Wisconsin, Madison) and J. B. Wilson. Glutamate metabolism in Brucella abortus strains of low and high virulence. J. Bacteriol. 84:911-915. 1962.-Brucella abortus strains of low virulence oxidize glutamate at a high rate, whereas strains of high virulence oxidize glutamate at a relatively low rate. Results indicated that this observation was not related to differences in pathway of glutamate oxidation or to differences in total enzyme activity. Permeability studies showed that the maximal rates of glutamate accumulation were 2.8 mumoles per 2 min per g (wet wt) for a strain of high virulence and 6.2 mumoles per 2 min per g for a strain of low virulence, but equal intracellular steady-state concentrations were attained by both types of strains. Evidence is presented which suggests that the site of glutamate oxidation is separate from the pool of glutamate being measured. Unequal rates of permeability at these sites could be the reason for the differences in rate of glutamate oxidation.
D002003:Brucella abortus; D005971:Glutamates; D018698:Glutamic Acid; D010084:Oxidation-Reduction; D014774:Virulence
D005971:Glutamates; D018698:Glutamic Acid
BRUCELLA ABORTUS; GLUTAMATES