ACTION OF A HISTIDINE ANALOGUE, 1,2,4-TRIAZOLE-3-ALANINE, IN SALMONELLA TYPHIMURIUM.
Levin, Alfred P. (The Johns Hopkins University, Baltimore, Md.), and Philip E. Hartman. Action of a histidine analogue, 1,2,4-triazole-3-alanine, in Salmonella typhimurium. J. Bacteriol. 86:820-828. 1963.-The effect of the histidine analogue, 1,2,4-triazole-3-alanine (TRA), on growth and enzyme synthesis in histidine auxotrophs of Salmonella typhimurium has been studied. TRA allows an increase of approximately 50% in the amount of protein in a culture but does not allow concomitant synthesis of ribonucleic acid and deoxyribonucleic acid. Although the analogue prevents the formation of active bacteriophage and of enzymatically active inosine 5'-phosphate dehydrogenase, it does not prevent the formation of enzymatically active l-histidinol phosphate phosphatase or of imidazoleacetol phosphate transaminase, two enzymes involved in the biosynthesis of histidine. Of the three known functions of histidine in the cell, TRA mimics two: it is incorporated into protein, and it acts as a repressor material for synthesis of enzymes involved in the formation of histidine. TRA fails to act as a feedback inhibitor of the first step in the formation of histidine.
D000409:Alanine / Q000031:analogs & derivatives; D001393:Azoles; D006639:Histidine; D009154:Mutation; D010088:Oxidoreductases; D010744:Phosphoric Monoester Hydrolases; D011492:Protein Hydrolysates; D011506:Proteins / Q000378:metabolism*; D011687:Purines; D012106:Research; D012486:Salmonella typhimurium; D000637:Transaminases
D001393:Azoles; D011492:Protein Hydrolysates; D011506:Proteins; D011687:Purines; C003633:triazolealanine; D006639:Histidine; D010088:Oxidoreductases; D000637:Transaminases; D010744:Phosphoric Monoester Hydrolases; D000409:Alanine; C030985:purine
ALANINE; AMINOTRANSFERASES; AZOLES; EXPERIMENTAL LAB STUDY; HISTIDINE; MUTATION; PHOSPHATASES; PROTEIN HYDROLYSATES; PROTEIN METABOLISM; PURINES; SALMONELLA TYPHIMURIUM