METABOLISM OF OMEGA-AMINO ACIDS. V. ENERGETICS OF THE  GAMMA-AMINOBUTYRATE FERMENTATION BY CLOSTRIDIUM AMINOBUTYRICUM.
Hardman, John K. (National Heart Institute, National Institutes of Health, Bethesda, Md.) and Thressa C. Stadtman. Metabolism of omega-amino acids. V. Energetics of the gamma-aminobutyrate fermentation by Clostridium aminobutyricum. J. Bacteriol. 85:1326-1333. 1963.-Clostridium aminobutyricum utilizes gamma-aminobutyrate as its sole carbon, nitrogen, and energy source, producing ammonia, acetate, and butyrate as a result of this fermentation. Coenzyme A (CoA)-transferase, phosphotransacetylase, and acetokinase activities have been demonstrated in crude extracts of the organism; the coupling of the reactions catalyzed by these enzymes to the fermentation reactions provides a mechanism whereby C. aminobutyricum can obtain energy, in the form of adenosine triphosphate, from the decomposition of gamma-aminobutyrate. Indirect evidence of additional phosphorylation, at the electron-transport level, has been obtained from molar growth yield studies and from the inhibition by 2,4-dinitrophenol of butyrate synthesis from gamma-aminobutyrate and from crotonyl-CoA.
D019297:2,4-Dinitrophenol; D000085:Acetates; D000214:Acyl Coenzyme A; D000255:Adenosine Triphosphate; D000596:Amino Acids; D000613:Aminobutyrates; D000641:Ammonia; D002087:Butyrates; D003013:Clostridium; D003065:Coenzyme A; D015256:Coenzyme A-Transferases; D004140:Dinitrophenols; D005285:Fermentation; D010770:Phosphotransferases; D011506:Proteins / Q000378:metabolism*; D012106:Research; D014166:Transferases; D014481:United States
D000085:Acetates; D000214:Acyl Coenzyme A; D000596:Amino Acids; D000613:Aminobutyrates; D002087:Butyrates; D004140:Dinitrophenols; D011506:Proteins; D000641:Ammonia; D000255:Adenosine Triphosphate; C010701:crotonyl-coenzyme A; D014166:Transferases; D010770:Phosphotransferases; D015256:Coenzyme A-Transferases; D019297:2,4-Dinitrophenol; D003065:Coenzyme A
ACETATES; ADENOSINE TRIPHOSPHATE; AMINOBUTYRIC ACID; AMMONIA; BUTYRATES; CLOSTRIDIUM; COENZYME A; DINITROPHENOLS; EXPERIMENTAL LAB STUDY; FERMENTATION; PHOSPHOTRANSFERASES; PROTEIN METABOLISM; TRANSFERASES