EFFECTS OF MALIGNANT EFFUSIONS ON THE MITOTIC INDEX   OF L STRAIN MOUSE CELLS GROWN IN TISSUE CULTURE.
By employing a clone of L strain mouse fibroblasts (L(E)) which does not exhibit cell clumping and lysis (cytolytic antibody reaction), it was possible to screen for the presence of growth-regulating factors in human sera and effusions, exclusive of an antigen-antibody reaction. Under conditions of the test a mitotic index greater than 20% indicated the presence of a growth-promoting factor.A total of 11 pleural effusions was tested. Four of the eight malignant effusions possessed a growth-promoting factor, while none of the three non-malignant effusions or the one sample of human umbilical cord serum possessed such a factor. Overnight storage of the unfiltered effusions at 5 degrees C. resulted in complete loss of the growthpromoting activity.
D000818:Animals; D002455:Cell Division; D002999:Clone Cells; D003078:Colchicine; D003581:Cytodiagnosis; D005122:Exudates and Transudates; D006801:Humans; D051379:Mice; D008940:Mitotic Index; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology*; D010996:Pleural Effusion; D016066:Pleural Effusion, Malignant; D012106:Research; D046509:Tissue Culture Techniques
D003078:Colchicine
CELL DIVISION; COLCHICINE; CYTODIAGNOSIS; EXPERIMENTAL LAB STUDY; EXUDATES AND TRANSUDATES; MICE; NEOPLASM DIAGNOSIS; NEOPLASM IMMUNOLOGY; PLEURAL EFFUSION; TISSUE CULTURE