INDUCTION AND REPRESSION OF L-ARABINOSE ISOMERASE IN  PEDIOCOCCUS PENTOSACEUS.
Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Induction and repression of l-arabinose isomerase in Pediococcus pentosaceus. J. Bacteriol. 85:1350-1355. 1963.-The inducible l-arabinose isomerase of Pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. The enzyme is measured by its ability to catalyze the isomerization of l-arabinose to l-ribulose. Whole cells suspended in a suitable buffer and pretreated with toluene were shown to exhibit this isomerase activity at a level comparable with that observed in cell-free enzyme preparations. Conditions for optimal induction of l-arabinose isomerase are described. In addition, it was determined that the formation of this enzyme is subject to repression by glucose, i.e., via catabolite repression.
D019747:Aldose-Ketose Isomerases; D001089:Arabinose; D050260:Carbohydrate Metabolism; D004791:Enzyme Inhibitors; D005947:Glucose; D007535:Isomerases; D010376:Pediococcus; D010429:Pentoses; D012106:Research; D046509:Tissue Culture Techniques; D014050:Toluene
D004791:Enzyme Inhibitors; D010429:Pentoses; D014050:Toluene; C100182:ribulose; D001089:Arabinose; D007535:Isomerases; D019747:Aldose-Ketose Isomerases; C019714:D-arabinose isomerase; C019715:L-arabinose isomerase; D005947:Glucose
ARABINOSE; CARBOHYDRATE METABOLISM; ENZYME INHIBITORS; EXPERIMENTAL LAB STUDY; ISOMERASES; PEDIOCOCCUS; TISSUE CULTURE; TOLUENE