DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON.
Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resistance to tetracycline (TC), chloramphenicol, streptomycin, and sulfonamide by cell-to-cell contact, were found to be transduced in the system of Salmonella E group with phage epsilon (epsilon(15) and epsilon(34)). The R(+) transductants of S. newington (S-84) and S. chittagong (S-224) were all found to be unable to transfer their R factors by conjugation, and their R factors were not eliminated by treatment with acridine dyes so far as tested. The R factors containing TC resistance were consistently segregated when transduced. At low multiplicities of infection, the R(+) transductants with epsilon(15) were all nonlysogenic and unable to produce normal epsilon(15) phage particles; among the R(+) transductants with epsilon(34), 34% were lysogenic and 66% were sensitive to epsilon(34).
D000166:Acridines; D000900:Anti-Bacterial Agents; D001435:Bacteriophages; D001459:Bangladesh; D002701:Chloramphenicol; D004096:Dihydrostreptomycin Sulfate; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae; D004926:Escherichia coli; D007106:Immune Sera; D007564:Japan; D011815:R Factors; D011817:Rabbits; D012106:Research; D012475:Salmonella; D012483:Salmonella Phages; D013307:Streptomycin; D013444:Sulfisoxazole; D013752:Tetracycline
D000166:Acridines; D000900:Anti-Bacterial Agents; D007106:Immune Sera; C077681:Camedon; D002701:Chloramphenicol; D013444:Sulfisoxazole; D013752:Tetracycline; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin
ACRIDINES; CHLORAMPHENICOL; DIHYDROSTREPTOMYCIN; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; IMMUNE SERUMS; RABBITS; SALMONELLA; SALMONELLA PHAGES; SULFISOXAZOLE; TETRACYCLINE