ANTISERUMS PREPARED WITH ACRYLAMIDE GEL USED AS ADJUVANT.
Antiserums to macroglobulins were prepared by a convenient and time-saving procedure in which acrylamide gel was used as adjuvant. After electrophoresis in acrylamide gel, the macroglobulin zone from the gel pattern was homogenized and injected directly into rabbits without intermediate isolation of the purified antigen. The resulting antiserums exhibited high titers of antiserum to macroglobulin and showed only weak cross-reactions with other gamma globulins in serologic tests.
D020106:Acrylamide; D000276:Adjuvants, Immunologic; D000577:Amides; D000818:Animals; D000941:Antigens; D002623:Chemistry Techniques, Analytical; D003429:Cross Reactions; D005782:Gels; D006736:Horses; D007106:Immune Sera; D011817:Rabbits; D012106:Research; D012712:Serum Globulins; D005719:gamma-Globulins
D000276:Adjuvants, Immunologic; D000577:Amides; D000941:Antigens; D005782:Gels; D007106:Immune Sera; D012712:Serum Globulins; D005719:gamma-Globulins; D020106:Acrylamide
AMIDES; CHEMISTRY, ANALYTICAL; EXPERIMENTAL LAB STUDY; GELS; HORSES; IMMUNE SERUMS; RABBITS; SERUM GLOBULINS