DOUBLE-STRANDED RIBONUCLEIC ACID FORMATION IN  VITRO BY MS 2 PHAGE-INDUCED RNA SYNTHETASE.
Part of the RNA synthesized from nucleoside triphosphate precursors by partially purified RNA synthetase, an enzyme induced in Escherichia coli by the RNA-containing phage MS 2, is resistant to hydrolysis by ribonuclease. Upon heating in 0.15M sodium chloride, 0.015M sodium citrate followed by fast cooling the material becomes ribonuclease-sensitive with a sharp transition at 102 degrees to 104 degrees C. The suggestion that the ribonuclease-resistant product is double-stranded RNA is reinforced by restoration of the ribonuclease resistance of the heat-denatured material by reannealing at temperatures just below the transition point and by its buoyant density in cesium sulfate. It is suggested that double-stranded RNA is the replicative form of MS 2 phage RNA.
D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages; D004926:Escherichia coli; D066298:In Vitro Techniques; D008025:Ligases; D012313:RNA; D011130:RNA Ligase (ATP); D012317:RNA Phages; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012106:Research; D012260:Ribonucleases
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012313:RNA; D012260:Ribonucleases; D008025:Ligases; D011130:RNA Ligase (ATP)
CHEMISTRY; COLIPHAGES; EXPERIMENTAL LAB STUDY; LIGASES; RNA, VIRAL