DNA SEGREGATION IN ESCHERICHIA COLI: OBSERVATIONS BY MEANS OF TRITIATED THYMIDINE DECAY.
Escherichia coli, strain 15(T-L-), inactivation by H(3)-thymidine decay was used in a study of DNA segregation. Two characteristics of the inactivation curves due to H(3)-thymidine decay, the initial slope and stable fraction, enable the mode of segregation to be defined. The results suggest that the segregation pattern is consistent with a semiconservative model of DNA replication. The data on the stable fraction indicate that the DNA of strain 15(T-L-), under our experimental conditions, may be organized into one duplex structure. Since colony-forming ability was used as the means of assay, it is possible to compare these experiments with those in which DNA segregation is examined on a structural basis.
D004247:DNA; D004261:DNA Replication; D004269:DNA, Bacterial; D004926:Escherichia coli; D012106:Research; D013936:Thymidine; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D004247:DNA; D013936:Thymidine
DNA, BACTERIAL; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; THYMIDINE; TRITIUM