HYBRIDIZATION OF HALF MOLECULES OF RABBIT GAMMA GLOBULIN.
Specifically purified rabbit antiovalbumin and normal gamma-globulin labeled with 1-131 were dissociated into half molecules by reduction and acidification. When a mixture of the two preparations was neutralized, a large proportion of mixed molecules having active combining sites and the same sedimentation coefficient as the original gamma-globulins was formed. Since the sulfhydryl groups were inactivated after reduction, the recombined subunits appear to be linked by noncovalent bonds.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006824:Hybridization, Genetic; D011817:Rabbits; D012106:Research; D013438:Sulfhydryl Compounds; D005719:gamma-Globulins
D013438:Sulfhydryl Compounds; D005719:gamma-Globulins
CHEMISTRY; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN; HYBRIDIZATION; RABBITS