BROMINE ANALYSIS IN 5-BROMOURACIL-LABELED DNA BY X-RAY FLUORESCENCE.
The degree of replacement of thymine by 5-bromouracil in the deoxyribonucleic acid (DNA) of the thymine-deficient bacterium Escherichia coli B 15 T(-) has been determined by a nondispersive x-ray fluorescence method. A measure of the fraction by weight of bromine in the purified DNA is required for determining the degree of thymine replacement. This fraction is experimentally determined on 35-microg samples of DNA by x-ray spectroanalysis.
D001966:Bromine; D001976:Bromouracil; D004247:DNA; D004269:DNA, Bacterial; D004926:Escherichia coli; D005453:Fluorescence; D012313:RNA; D012329:RNA, Bacterial; D011827:Radiation; D012106:Research; D013941:Thymine; D014498:Uracil; D014965:X-Rays
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D001976:Bromouracil; D014498:Uracil; D012313:RNA; D004247:DNA; D013941:Thymine; D001966:Bromine
BROMINE; ESCHERICHIA COLI; FLUORESCENCE; RADIATION; RNA, BACTERIAL; URACIL