2,4-DINITROPHENOL: LACK OF INTERACTION WITH HIGH-ENERGY INTERMEDIATES OF OXIDATIVE PHOSPHORYLATION.
If the uncoupling agent 2,4-dinitrophenol is added just prior to addition of the phosphate acceptor adenosine diphosphate to rat liver mitochondria previously incubated with substrate, orthophosphate, and oxygen, steady-state phosphorylation is abolished, whereas the initial phase of rapid phosphorylation, the so-called "adenosine triphosphate jump," is undisturbed. This leads to the conclusion that uncoupling agents operate by interfering with the synthesis of high-energy intermediates, not by hydrolyzing or otherwise inactivating them.
D019297:2,4-Dinitrophenol; D000227:Adenine Nucleotides; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004140:Dinitrophenols; D008099:Liver / Q000166:cytology*; D008660:Metabolism; D008928:Mitochondria; D008930:Mitochondria, Liver; D010085:Oxidative Phosphorylation; D010710:Phosphates; D051381:Rats; D012106:Research
D000227:Adenine Nucleotides; D004140:Dinitrophenols; D010710:Phosphates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019297:2,4-Dinitrophenol
ADENINE NUCLEOTIDES; ADENOSINE TRIPHOSPHATE; DINITROPHENOLS; EXPERIMENTAL LAB STUDY; LIVER CYTOLOGY; METABOLISM; MITOCHONDRIA; RATS