ASSOCIATION OF "NATURAL" ANTIBODIES TO GRAM-NEGATIVE BACTERIA WITH THE GAMMA-1-MACROGLOBULINS.
"Natural" antibodies to representative species of Enterobacteriaceae were found to be principally associated with the gamma(1)-macroglobulin prepared from Cohn fraction III-I of pooled human plasma. The amount of antibodies present was estimated by measuring the bactericidal, hemagglutinating, mouse-protective, and opsonophagocytic activities of this fraction. Comparison with the antibody activity of gamma(2)-globulin of Cohn fraction II showed that up to 100 times more antibody activity on a weight basis was present in the macroglobulin fraction. These findings suggest that differences between natural and immune antibody result from the physicochemical properties of 2 different classes of immune globulin.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D004926:Escherichia coli; D006384:Hemagglutination; D006801:Humans; D007709:Klebsiella; D008259:Macroglobulins; D051379:Mice; D010587:Phagocytosis; D011550:Pseudomonas aeruginosa; D012106:Research; D012485:Salmonella typhi; D012712:Serum Globulins; D012760:Shigella; D005719:gamma-Globulins
D000906:Antibodies; D008259:Macroglobulins; D012712:Serum Globulins; D005719:gamma-Globulins
ANTIGEN-ANTIBODY REACTIONS; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN; HEMAGGLUTINATION; KLEBSIELLA; MICE; PHAGOCYTOSIS; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHOSA; SERUM GLOBULINS; SHIGELLA