IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO.
Phytohemagglutinin causes cultured lymphocytes to agglutinate, divide, and produce gamma-globulin. Most cells are transformed into large lymphocytes, some resembling plasmocytes. Actinomycin D stops gamma-globulin production after 2 hours. When specific antigens are added to lymphocytes from sensitized individuals, only some cells undergo morphological transformation, produce gamma-globulin, and divide. When cells or cell extracts from an unrelated individual are added to a culture, a similar reaction occurs.
D000941:Antigens; D002455:Cell Division; D003609:Dactinomycin; D004168:Diphtheria Toxoid; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes; D008938:Mitosis; D010406:Penicillins; D010567:Pertussis Vaccine; D010600:Pharmacology; D037121:Plant Lectins; D010950:Plasma Cells; D012106:Research; D014373:Tuberculin
D000941:Antigens; D004168:Diphtheria Toxoid; D010406:Penicillins; D010567:Pertussis Vaccine; D037121:Plant Lectins; D014373:Tuberculin; D003609:Dactinomycin
ACTINOMYCIN; ANTIGENS; CELL DIVISION; DIPHTHERIA TOXOID; EXPERIMENTAL LAB STUDY; LYMPHOCYTES; PENICILLIN; PERTUSSIS VACCINE; PHARMACOLOGY; PLANT AGGLUTININS; TUBERCULIN