EFFECTS OF PROTEIN, LIPIDS, AND SURFACTANTS ON THE ANTIMICROBIAL ACTIVITY OF SYNTHETIC STEROIDS.
Three 4-azacholestanes and two A-norcholestanes were inactivated by 10 and 20% bovine serum and by 1.0, 2.5, and 5.0% sheep blood. The five compounds exhibited hemolytic properties when tested with 2% sheep blood and 2% human blood. These cholestanes inhibited Streptococcus pyogenes and were completely inactivated by 0.1% lecithin. Tween 80 was comparable to lecithin in causing the inactivation of steroids; 1% polyethylene glycol-4000 was inert; 1% Tween 20 and 1.0% Span 20 caused the inactivation of 3beta,4-dimethyl-4-aza-5alpha-cholestane (ND-307). The sodium salts of four fatty acids, oleate, stearate, deoxycholate, and lauryl sulfate (0.1 to 1.0 mg/ml), effectively interfered with the action of ND-307. The steroids appear to have some properties similar to those of antimicrobial surfactants of the cationic type but have certain distinct features.
D000818:Animals; D000890:Anti-Infective Agents; D002412:Cations; D002417:Cattle; D005227:Fatty Acids; D006801:Humans; D054709:Lecithins; D008055:Lipids; D010600:Pharmacology; D010713:Phosphatidylcholines; D011136:Polysorbates; D012106:Research; D012440:Saccharomyces; D012756:Sheep; D012967:Sodium Dodecyl Sulfate; D013256:Steroids; D013297:Streptococcus pyogenes; D013501:Surface-Active Agents
D000890:Anti-Infective Agents; D002412:Cations; D005227:Fatty Acids; D054709:Lecithins; D008055:Lipids; D010713:Phosphatidylcholines; D011136:Polysorbates; D013256:Steroids; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate
EXPERIMENTAL LAB STUDY; FATTY ACIDS; LECITHINS; PHARMACOLOGY; SACCHAROMYCES; STEROIDS; STREPTOCOCCUS PYOGENES; SURFACE-ACTIVE AGENTS