BETA-GLUCOSIDASE SYSTEM OF NEUROSPORA CRASSA. I. BETA-GLUCOSIDASE AND CELLULASE ACTIVITIES OF MUTANT AND WILD-TYPE STRAINS.
Eberhart, Bruce (University of North Carolina, Greensboro), David F. Cross, and Lewis R. Chase. beta-Glucosidase system of Neuspora crassa. I. beta-Glucosidase and cellulose activities of mutant and wild-type strains. J. Bacteriol. 87:761-770. 1964.-A mutant strain, gluc-1, of Neurospora crassa was isolated and characterized by its low level of beta-glucosidase activity. The mutant was selected by testing irradiated colonies for extracellular beta-glucosidase activity. Strains containing the gluc-1 gene were also visibly detected by their reduced ability to destroy esculin in their growth media. The mutant strain grew at wild-type rates with cellobiose or carboxymethylcellulose as carbon sources. This auxotrophic similarity with wild type is explained by the presence of at least two beta-glucosidases (and possibly two cellulases) in Neurospora that act complementarily. The thermolabile beta-glucosidase was destroyed after 1 min of incubation at 60 C. This enzyme was present in mycelia but absent in conidial extracts. A second beta-glucosidase that is comparatively stable at 60 C was present in both mycelia and conidia. A partial separation of these enzymes was achieved with ammonium fractionation of mycelial extracts of gluc-1 and wild-type strains. Thermolabile beta-glucosidase and cellulase activity appear not to be affected by the gluc-1 mutation, whereas the thermostable glucosidase is greatly reduced in gluc-1 strains.
D001431:Bacteriological Techniques; D001669:Biochemical Phenomena; D001671:Biochemistry; D002475:Cellobiose; D044602:Cellulases; D002482:Cellulose; D005419:Flavonoids; D005823:Genetics; D005959:Glucosidases; D008660:Metabolism; D009154:Mutation; D009491:Neurospora; D009492:Neurospora crassa; D012106:Research; D001617:beta-Glucosidase
D005419:Flavonoids; D002475:Cellobiose; D002482:Cellulose; D044602:Cellulases; D005959:Glucosidases; D001617:beta-Glucosidase
BACTERIOLOGICAL TECHNICS; BIOCHEMISTRY; BIOFLAVONOIDS; CELLULOSE; EXPERIMENTAL LAB STUDY; GENETICS; GLUCOSIDASE; METABOLISM; MUTATION; NEUROSPORA