N-ETHYLMALEIMIDE INHIBITION OF HORSESHOE CRAB HEMOCYTE AGGLUTINATION.
The sulfhydryl inhibitor N-ethylmaleimide (NEM) inhibits the agglutination of horseshoe crab hemocytes. The inhibitory action is neutralized by adding cysteine to the NEM before it is mixed with the hemolymph. This new method for arresting horseshoe crab hemocyte agglutination suggests the participation of sulfhydryl groups in the process.
D000371:Agglutination; D000818:Animals; D003445:Crustacea; D005033:Ethylmaleimide; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006434:Hemocytes; D006458:Hemolymph; D006737:Horseshoe Crabs; D037102:Lectins; D010600:Pharmacology; D011758:Pyrroles; D012106:Research
D037102:Lectins; D011758:Pyrroles; D005033:Ethylmaleimide
CRUSTACEA; EXPERIMENTAL LAB STUDY; HEMAGGLUTINATION; HEMOLYMPH; PHARMACOLOGY; PYRROLES