PREFERENTIAL SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS IN THE PRESENCE OF 5-METHYL-TRYPTOPHAN.
Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferentially synthesized alpha-amylase in the presence of 5-methyl-tryptophan (5-MT). 5-MT did not inhibit the formation of active alpha-amylase or the incorporation of proline-C(14) into alpha-amylase. In contrast, p-fluorophenylalanine inhibited the formation of active alpha-amylase by 92% and the incorporation of proline-C(14) into the enzyme by 95%. p-Fluorophenylalanine and 5-MT inhibited cellular protein synthesis, as measured by proline-C(14) incorporation, by 74 and 72%, respectively.
D000681:Amylases; D000963:Antimetabolites; D001407:Bacillus; D001431:Bacteriological Techniques; D001411:Geobacillus stearothermophilus; D008320:Maltose; D008660:Metabolism; D010649:Phenylalanine; D011392:Proline; D011506:Proteins / Q000378:metabolism*; D012106:Research; D014364:Tryptophan / Q000031:analogs & derivatives; D000516:alpha-Amylases
D000963:Antimetabolites; D011506:Proteins; C004182:5-methyltryptophan; D010649:Phenylalanine; D008320:Maltose; D014364:Tryptophan; D011392:Proline; D000681:Amylases; D000516:alpha-Amylases
AMYLASE; ANTIMETABOLITES; BACILLUS; BACTERIOLOGICAL TECHNICS; EXPERIMENTAL LAB STUDY; METABOLISM; PHENYLALANINE; PROLINE; PROTEIN METABOLISM; TRYPTOPHAN