SPLEEN-COLONY FORMATION IN ANEMIC MICE OF GENOTYPE WW.
The hemopoietic cells from anemic mice of genotype WW are less able by 200-fold to take part in colony formationin the spleen than cells from the normal littermates of genotype ww. The genetic defect shows itself in the colony-forming cells, since cells from normal littermate mice formcolonies in the spleens of unirradiated mice of genotype WW. Use of animals of genotype WVVWT as recipients improves the spleen-colony method by removing bias resulting fromthe death of irradiated recipients.
D000740:Anemia; D000748:Anemia, Macrocytic; D000818:Animals; D016026:Bone Marrow Transplantation; D003585:Cell Biology; D004920:Erythropoiesis; D005838:Genotype; D051379:Mice; D011830:Radiation Effects / Q000276:immunology*; D012106:Research; D013154:Spleen; D046509:Tissue Culture Techniques; D014181:Transplantation Immunology
ANEMIA, MACROCYTIC; BONE MARROW TRANSPLANTATION; CYTOLOGY; ERYTHROPOIESIS; EXPERIMENTAL LAB STUDY; MICE; RADIATION EFFECTS; RADIATION IMMUNOLOGY; SPLEEN; TISSUE CULTURE; TRANSPLANTATION IMMUNOLOGY