TRYPSINIZATION OF ANIMAL TISSUES FOR CELL CULTURE: THEORETICAL CONSIDERATIONS AND AUTOMATIC APPARATUS.
The theoretical background and the experimental feasibility of an automatic control for the continuous trypsinization process are discussed. An automatic apparatus is described with experimental evidence that the optimal mean residence time of monkey kidney cells in the trypsinization flask is between 5 and 8 min, in a volume of fluid approximately ten times that of the tissue processed. A temperature of 36 C and a pH of 7.8 provide optimal conditions for cell viability.
D000818:Animals; D018929:Cell Culture Techniques; D002467:Cell Nucleus; D004864:Equipment and Supplies; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D007159:Immunologic Tests; D007668:Kidney; D012106:Research; D046509:Tissue Culture Techniques; D014357:Trypsin
D014357:Trypsin
CELL NUCLEUS; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; KIDNEY; MONKEYS; TISSUE CULTURE; TRYPSIN