COMPOSITION OF FOWLPOX VIRUS AND INCLUSION MATRIX.
Randall, Charles C. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, Robert W. Darlington, and James M. Hyde. Composition of fowlpox virus and inclusion matrix. J. Bacteriol. 87:939-944. 1964.-Inclusion bodies of fowlpox virus infection are especially favorable starting material for the isolation of virus and inclusion matrix. Electron micrographs of viral particles and matrix indicated a high degree of purification. Density-gradient centrifugation of virus in cesium chloride and potassium tartrate was unsatisfactory because of inactivation, and clumping or disintegration. Chemical analyses of virus and matrix revealed significant amounts of lipid, protein, and deoxyribonucleic acid, but no ribonucleic acid or carbohydrate. Approximately 47% of the weight of the virus and 83% of the matrix were extractable in chloroform-methanol. The lipid partitions of the petroleum ether extracts were similar, except that the phospholipid content of the matrix was 2.2 times that of the virus. Viral particles were sensitive to diethyl ether and chloroform.
D000818:Animals; D002498:Centrifugation; D002623:Chemistry Techniques, Analytical; D002784:Cholesterol; D004247:DNA; D004279:DNA, Viral; D004583:Electrons; D005227:Fatty Acids; D005587:Fowlpox virus; D005989:Glycerides; D002479:Inclusion Bodies; D008055:Lipids; D008853:Microscopy; D008854:Microscopy, Electron; D010743:Phospholipids; D010758:Phosphorus; D011212:Poxviridae; D011506:Proteins; D012106:Research
D004279:DNA, Viral; D005227:Fatty Acids; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D011506:Proteins; D010758:Phosphorus; D004247:DNA; D002784:Cholesterol
CENTRIFUGATION; CHEMISTRY, ANALYTICAL; CHOLESTEROL; DNA, VIRAL; EXPERIMENTAL LAB STUDY; FATTY ACIDS; GLYCERIDES; INCLUSION BODIES; MICROSCOPY, ELECTRON; PHOSPHOLIPIDS; PHOSPHORUS; POXVIRUS; PROTEINS