AN IMPROVED METHOD FOR THE SIMPLE AND ACCURATE COLORIMETRIC DETERMINATION OF UREA WITH EHRLICH'S REAGENT.
An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12.5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow colour. The determination is accurate, very sensitive and rapid, taking place in only 10 minutes.
D001547:Benzaldehydes; D001774:Blood Chemical Analysis; D003124:Colorimetry; D006801:Humans; D007202:Indicators and Reagents; D014508:Urea; D014556:Urine
D001547:Benzaldehydes; D007202:Indicators and Reagents; D014508:Urea; C510241:p-dimethylaminobenzaldehyde
BLOOD CHEMICAL ANALYSIS; COLORIMETRY; INDICATORS AND REAGENTS; UREA; URINE