LACK OF INFLUENCE OF SUSPENDING MEDIA ON HEAT ACTIVATION OF BACILLUS SUBTILIS SPORES AND ABSENCE OF DEACTIVATION.
Bacillus subtilis strain 5230 endospores suspended in a variety of suspending media at a concentration of ca. 10(8) per ml were heated at 95 and 75 C. The effect of the heating at 75 C was measured by plate count, and was reported as the heat-activated decimal fraction of the total viable-spore population. Thermal inactivation at 95 C was influenced by the suspending medium. No effects on heat activation at 75 C were noted for suspending media containing glucose, xylose, ribose, NaCl, or sodium phosphate, nor was there any marked effect due to a change in pH from 5 to 8. The heat-activation response was retained during postheating storage at 5 C in water up to 215 days. Postheating storage in several suspending media for 7 days also indicated no deactivation.
D001412:Bacillus subtilis; D003470:Culture Media; D005079:Excipients; D005947:Glucose; D006361:Heating; D006358:Hot Temperature; D010710:Phosphates; D012106:Research; D012965:Sodium Chloride; D013171:Spores, Bacterial
D003470:Culture Media; D005079:Excipients; D010710:Phosphates; D012965:Sodium Chloride; D005947:Glucose; C018279:sodium phosphate
BACILLUS SUBTILIS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; HEAT