PATHWAYS FOR BIOSYNTHESIS OF A BACTERIAL CAPSULAR POLYSACCHARIDE. IV. CAPSULE RESYNTHESIS BY DECAPSULATED RESTING-CELL SUSPENSIONS.
Juni, Elliot (Emory University, Atlanta, Ga.), and Gloria A. Heym. Pathways for biosynthesis of a bacterial capsular polysaccharide. IV. Capsule resynthesis by decapsulated resting-cell suspensions. J. Bacteriol. 87:461-467. 1964.-Methods were devised for stripping capsules from encapsulated bacteria. By use of stripped resting-cell suspensions of a gram-negative capsule-forming coccus, it was shown that polysaccharide capsule resynthesis depends upon the presence of air and an oxidizable substrate. Capsule resynthesis proceeds linearly with time. For a given quantity of stripped cells, the net amount of polysaccharide capsule synthesized is a linear function of the amount of substrate oxidized. The only factor that appears to limit the extent of capsule synthesis by resting cells is the amount of substrate utilized. A series of photomicrographs of wet mounts made in India ink show the appearance of stripped cells and resynthesized capsules formed during oxidation of pyruvate and glucose by stripped resting-cell suspensions.
D000085:Acetates; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D005947:Glucose; D007281:Ink; D007773:Lactates; D008293:Malates; D008365:Manometry; D008853:Microscopy; D008858:Microscopy, Phase-Contrast; D010429:Pentoses; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D012106:Research; D013535:Suspensions
D000085:Acetates; D002247:Carbon Isotopes; D007773:Lactates; D008293:Malates; D010429:Pentoses; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D013535:Suspensions; D005947:Glucose
ACETATES; CARBOHYDRATE METABOLISM; CARBON ISOTOPES; EXPERIMENTAL LAB STUDY; GLUCOSE; INK; LACTATES; MALATES; MANOMETRY; MICROSCOPY, PHASE CONTRAST; PENTOSES; POLYSACCHARIDES, BACTERIAL; PYRUVATES