HUMAN INFLUENZA A VIRUSES: RAPID PLAQUE ASSAY IN HAMSTER KIDNEY CELLS.
The production of focal necrosis in hamster kidney cells maintained beneath an overlay of semisolid agar medium free of serum permits rapid, reproducible, quantitative assay for the three subtypes of influenza A viruses. Adaptation of virus to these cells was not required. A straight-line dose-response relationship was observed betweeen the number of plaques produced and the relative concentration of virus. Specificity was demonstrated by quantitative neutralization with immune serum.
D003470:Culture Media; D004847:Epithelial Cells; D007106:Immune Sera; D007159:Immunologic Tests; D009980:Influenza A virus; D007668:Kidney; D009975:Orthomyxoviridae; D012106:Research; D046509:Tissue Culture Techniques; D014776:Virus Cultivation
D003470:Culture Media; D007106:Immune Sera
EXPERIMENTAL LAB STUDY; INFLUENZA VIRUSES; TISSUE CULTURE; VIRUS CULTIVATION