MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS.
Eliminating most of the ionic calcium from growth medium does not affect cell growth, but it allows simple and preferential detachment of HeLa cells in mitosis from nearly confluent monolayers.
D002118:Calcium; D002455:Cell Division; D049109:Cell Proliferation; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D010600:Pharmacology; D012106:Research; D046509:Tissue Culture Techniques
D003470:Culture Media; D002118:Calcium
CALCIUM; CELL DIVISION; EXPERIMENTAL LAB STUDY; PHARMACOLOGY; TISSUE CULTURE