IMMUNOLOGIC RELATION OF STREPTOCOCCAL AND TISSUE ANTIGENS. II. CROSS-REACTION OF ANTISERA TO MAMMALIAN HEART TISSUE WITH A CELL WALL CONSTITUENT OF CERTAIN STRAINS OF GROUP A STREPTOCOCCI.
Further evidence of a cross-reactive relationship between a cell wall antigen of certain Group A streptococcal strains and mammalian heart tissue is provided by the demonstration that goat antisera to washed human or rabbit heart tissue homogenates exhibit precipitation with cell wall extract preparations. A single line of precipitation was observed in agar gel diffusion tests. Precipitating activity of goat antiheart sera was related to the gamma-globulin fraction in zone electrophoresis. The cell wall antigen was demonstrated in cell wall extracts of a Type 5 and Type 19 but not of a Type 12 strain. Antigenic activity was concentrated in the same neutral salt and chromatographic fractions of acid extracts of cell wall previously reported to exhibit this activity by immunofluorescent inhibition of the cross-reaction of antistreptococcal serum with heart tissue.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens; D002473:Cell Wall; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004586:Electrophoresis; D006041:Goats; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D009206:Myocardium; D011233:Precipitin Tests; D011817:Rabbits; D012106:Research; D013291:Streptococcus; D013297:Streptococcus pyogenes; D014020:Tissue Extracts
D000941:Antigens; D007106:Immune Sera; D014020:Tissue Extracts
ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; COMPLEMENT FIXATION TESTS; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GOATS; IMMUNE SERUMS; MYOCARDIUM; PRECIPITIN TESTS; RABBITS; STREPTOCOCCUS; TISSUE EXTRACTS