A COMPARISON OF A FLUORESCENT ANTIBODY TECHNIQUE WITH A CULTURAL METHOD IN THE DETECTION OF INFECTIONS WITH SHIGELLA SONNEI.
A comparison has been made of a fluorescent antibody technique with a cultural method for the detection of Sh. sonnei in faeces. The two methods were in agreement in 73% of the 394 specimens examined; 57 (14.5%) speciments were positive by culture only; 52 (13.2%) specimens gave positive results by fluorescence microscopy which were not confirmed by culture. Most of the latter are thought to be ;false positive' results.The value and usefulness of fluorescence microscopy in the diagnosis of Sonne dysentery is discussed.
D001431:Bacteriological Techniques; D003470:Culture Media; D046508:Culture Techniques; D005243:Feces; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D006801:Humans; D008853:Microscopy; D008856:Microscopy, Fluorescence; D012106:Research; D012760:Shigella; D012764:Shigella sonnei
D003470:Culture Media
BACTERIOLOGICAL TECHNICS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; FECES; FLUORESCENT ANTIBODY TECHNIC; MICROSCOPY, FLUORESCENCE; SHIGELLA; SHIGELLA SONNEI