THE ESTIMATION OF URANIN (FLUORESCEIN SODIUM) IN BLOOD.
The extraction and estimation of uranin (fluorescein sodium) in plasma is described. Heparin-plasma containing uranin is digested with crude ficin and extracted with acid n-pentanol. The supernatant fluid is re-extracted with N NaOH and, after volume adjustment, read against a uranin standard in a fluorimeter with appropriate filters. It appears reliable within 4% at concentrations as low as 10(-8) g./ml.
D001774:Blood Chemical Analysis; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D013053:Spectrophotometry
D005452:Fluoresceins; D019793:Fluorescein
BLOOD CHEMICAL ANALYSIS; FLUORESCEINS; SPECTROPHOTOMETRY