ELECTRON MICROSCOPIC AND BIOCHEMICAL STUDIES OF PYRUVATE DEHYDROGENASE COMPLEX OF ESCHERICHIA COLI.
Examination with the electron microscope of the pyruvate dehydrogenase complex and its component enzymes from Escherichia coli indicates that the complex has a polyhedral structure with a diameter of about 300 to 350 angstroms and a height of 200 to 250 angstroms. The reconstituted complex closely resembles the native complex in appearance. A tentative model of this multienzyme complex is proposed on the basis of the correlated biochemical and electron-microscopic data
D000085:Acetates; D000217:Acyltransferases; D002262:Carboxy-Lyases; D004396:Coloring Agents; D004583:Electrons; D004926:Escherichia coli; D008853:Microscopy; D008854:Microscopy, Electron; D010088:Oxidoreductases; D010772:Phosphotungstic Acid; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D012106:Research; D013194:Staining and Labeling; D008063:Thioctic Acid
D000085:Acetates; D004396:Coloring Agents; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D010772:Phosphotungstic Acid; D008063:Thioctic Acid; D010088:Oxidoreductases; D000217:Acyltransferases; D002262:Carboxy-Lyases
ACETATES; ACYLTRANSFERASES; CARBOXY-LYASES; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; LIPOIC ACID; MICROSCOPY, ELECTRON; OXIDOREDUCTASES; PHOSPHOTUNGSTIC ACID; PYRUVATES; STAINS AND STAINING