ANTIGEN-BINDING ACTIVITY OF 6S SUBUNITS OF BETA-2-MACROGLOBULIN ANTIBODY.
Direct evidence is provided for the antigen binding activity of the 6S subunits formed by reduction and alkylation of rabbit beta2-macroglobulin antibody. Binding activity of the subunits was clearly demonstrated by radioimmunoelectrophoresis with a preparation of purified rabbit antibody against the p-azobenzenearsonate group, which contained 40 percent of beta2M-antibody. The precipitate arc formed between the subunits and sheep antiserum against rabbit macroglobulin was shown to bind radioactive antigen specifically.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000941:Antigens; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008259:Macroglobulins; D011817:Rabbits; D012106:Research; D012756:Sheep; D014461:Ultracentrifugation
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D008259:Macroglobulins
ANTIGEN-ANTIBODY REACTIONS; EXPERIMENTAL LAB STUDY; IMMUNOELECTROPHORESIS; MACROGLOBULINS