TOBACCO MOSAIC VIRUS: CYTOLOGICAL EVIDENCE OF THE SYNTHESIS IN THE NUCLEUS.
Living tomato hair cells were directly injected with tobacco mosaic virus or tobacco mosaic virus RNA with a fine glass needle. Use of a fluorescent tobacco mosaic virus antibody showed the presence of the virus protein in the nucleus 6 to 18 hours after the injection. As time of infection progressed, staining of the cytoplasm became more prominent.
D002467:Cell Nucleus; D003593:Cytoplasm; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D008853:Microscopy; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000378:metabolism*; D012313:RNA; D012367:RNA, Viral; D012106:Research; D013194:Staining and Labeling; D014027:Tobacco Mosaic Virus; D014764:Viral Proteins
D011506:Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA
CELL NUCLEUS; EXPERIMENTAL LAB STUDY; FLUORESCENT ANTIBODY TECHNIC; MICROSCOPY, FLUORESCENCE; PROTEIN METABOLISM; RNA, VIRAL; TOBACCO MOSAIC VIRUS; VIRAL PROTEINS