IDENTIFICTION OF ACETYLCHOLINE IN SYMPATHETIC GANGLIA BY CHEMICAL AND PHYSICAL METHODS.
Extracts of sympathetic ganglionic chains contain a substance which behaves like acetylcholine biologically, chromatographically, and electrophoretically. The melting point of the tetrachloroaurate salt of this substance is identical to that of acetylcholine tetrachloroaurate. No other choline esters have been detected in these extracts. Perfusion of sympathetic ganglia with C(14)-choline indicates that C(14)-acetylcholine is released.
D000109:Acetylcholine; D000818:Animals; D001341:Autonomic Nervous System; D002247:Carbon Isotopes; D002417:Cattle; D002623:Chemistry Techniques, Analytical; D002794:Choline; D002845:Chromatography; D004586:Electrophoresis; D005724:Ganglia; D005725:Ganglia, Autonomic; D005728:Ganglia, Sympathetic; D012106:Research; D014020:Tissue Extracts
D002247:Carbon Isotopes; D014020:Tissue Extracts; D002794:Choline; D000109:Acetylcholine
ACETYLCHOLINE; CARBON ISOTOPES; CATTLE; CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GANGLIA, AUTONOMIC; TISSUE EXTRACTS