title: PULMONARY ALVEOLAR CELL INCLUSIONS: THEIR DEVELOPMENT IN THE RAT.
abstract: Cytoplasmic inclusions in the special pulmonary alveolar cells of adult, newborn, and fetal rats were investigated by electron microscopy and histochemistry, and lung extracts were analyzed for surface-tension activity. The inclusions were positive in the periodic acid-Schiff test after digestion with diastase and were not stained with toluidine blue; they formed myelin figures and possessed alkaline phosphatase activity. Both the typical surface tension activity and the inclusions developed 2 days before birth. These properties differentiated the inclusions from mitochondria.
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D004396:Coloring Agents; D004583:Electrons; D005333:Fetus; D006651:Histocytochemistry; D002479:Inclusion Bodies; D008168:Lung; D008853:Microscopy; D008854:Microscopy, Electron; D008928:Mitochondria; D010336:Pathology; D051381:Rats; D012106:Research
chemical_list: D004396:Coloring Agents; D000469:Alkaline Phosphatase
keywords: ALKALINE PHOSPHATASE; ANIMALS, NEWBORN; EXPERIMENTAL LAB STUDY; FETUS; HISTOCYTOCHEMISTRY; INCLUSION BODIES; LUNG; MICROSCOPY, ELECTRON; PATHOLOGY; RATS