LOBULOALVEOLAR DIFFERENTIATION IN MOUSE MAMMARY TISSUES IN VITRO.
Administration of lobuloalveolar mammogenic hormones (for a period insufficient to induce alveolar differentiation) to immature mice prior to cultivation of their mammary glands in chemically defined, hormone-supplemented media resulted in lobuloalveolar growth in vitro comparable to that of late pregnancy. No such growth occurred in cultures of mammary tissues from mice not treated with hormones. The use of tissues from hormone-treated donors might serve as a valuable tool for studies concerned with differentiation in vitro.
D000450:Aldosterone; D000818:Animals; D002454:Cell Differentiation; D004958:Estradiol; D005260:Female; D013006:Growth Hormone; D006653:Histology; D019382:Human Growth Hormone; D066298:In Vitro Techniques; D007328:Insulin; D008321:Mammary Glands, Animal; D051379:Mice; D010600:Pharmacology; D011247:Pregnancy; D011374:Progesterone; D011388:Prolactin; D012106:Research; D046509:Tissue Culture Techniques
D007328:Insulin; D019382:Human Growth Hormone; D000450:Aldosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone
ALDOSTERONE; ESTRADIOL; EXPERIMENTAL LAB STUDY; HISTOLOGY; INSULIN; MAMMAE; MICE; PHARMACOLOGY; PROGESTERONE; PROLACTIN; SOMATOTROPIN; TISSUE CULTURE