HISTOCHEMICAL DEMONSTRATION OF UPTAKE OF EXOGENOUS NOREPINEPHRINE BY ADRENERGIC FIBERS IN VITRO.
Adrenergic fibers in isolated iris preparations were demonstrated histochemically by means of the formaldehyde condensation technique. In the presence of adenosine triphosphate (ATP) there was a net uptake of norepinephrine added in vitro by the adrenergic fibers producing a striking increase in fluorescence. In the absence of ATP only a slight increase in fluorescence was observed when the tissue was exposed to high concentrations of norepinephrine.
D000255:Adenosine Triphosphate; D000320:Adrenergic Fibers; D005453:Fluorescence; D005557:Formaldehyde; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007498:Iris; D008853:Microscopy; D008856:Microscopy, Fluorescence; D009411:Nerve Endings; D009638:Norepinephrine; D010600:Pharmacology; D051381:Rats; D012106:Research; D013564:Sympathetic Nervous System
D005557:Formaldehyde; D000255:Adenosine Triphosphate; D009638:Norepinephrine
ADENOSINE TRIPHOSPHATE; EXPERIMENTAL LAB STUDY; FORMALDEHYDE; HISTOCYTOCHEMISTRY; IRIS; MICROSCOPY, FLUORESCENCE; NERVE ENDINGS; NOREPINEPHRINE; PHARMACOLOGY; RATS; SYMPATHETIC NERVOUS SYSTEM