TRYPTOPHAN SYNTHETASE FROM NEUROSPORA: A MODIFICATION IN THE REACTION SCHEME.
In addition to indole-3-glycerolphosphate, an unreported reaction product has been detected in incubation mixtures containing tryptophan synthetase from Neurospora, indole, and glyceraldehyde-3-phosphate. This product has been tentatively identified as an indole derivative. Since this compound is not formed in incubation mixtures initially containing only enzyme and indole-3-glycerolphosphate, it was concluded that indole-3-glycerolphosphate synthesis and breakdown are not simply the forward and reverse components of a reversible reaction.
D002845:Chromatography; D005994:Glycerophosphates; D006836:Hydro-Lyases; D007211:Indoles; D008660:Metabolism; D009154:Mutation; D009491:Neurospora; D012106:Research; D014364:Tryptophan; D014367:Tryptophan Synthase
D005994:Glycerophosphates; D007211:Indoles; C008117:indoleglycerol phosphate; C030374:indole; D014364:Tryptophan; D006836:Hydro-Lyases; D014367:Tryptophan Synthase
CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; GLYCEROPHOSPHATES; HYDRO-LYASES; INDOLES; METABOLISM; MUTATION; NEUROSPORA; TRYPTOPHAN