STAPHYLOCOCCAL ALPHA-HEMOLYSIN: DETECTION ON THE ERYTHROCYTE MEMBRANE BY IMMUNOFLUORESCENCE.
Purified staphylococcal alpha-hemolysin (but not the toxoid) was demonstrated on the surface of rabbit and human erythrocytes by immunofluorescence. This occurred during the period of maximal hemolysis and was a transient event. These findings have been analyzed in relation to previous data on the kinetics of leakage of both small and complex molecular constituents of the erythrocyte.
D000818:Animals; D000992:Antitoxins; D001427:Bacterial Toxins; D004910:Erythrocyte Membrane; D004912:Erythrocytes; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D006460:Hemolysin Proteins; D006461:Hemolysis; D006801:Humans; D008853:Microscopy; D008856:Microscopy, Fluorescence; D011817:Rabbits; D012106:Research; D013210:Staphylococcus; D014118:Toxins, Biological
D000992:Antitoxins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D014118:Toxins, Biological; C021091:staphylococcal alpha-toxin
ERYTHROCYTES; EXPERIMENTAL LAB STUDY; FLUORESCENT ANTIBODY TECHNIC; HEMOLYSINS; HEMOLYSIS; MICROSCOPY, FLUORESCENCE; RABBITS; STAPHYLOCOCCUS; TOXINS AND ANTITOXINS