title: THE MICRODETERMINATION OF BIOLOGICAL COPPER WITH OXALYLDIHYDRAZIDE.
abstract: Methods are described for the determination of microgram quantities of biological copper in aqueous medium as the intensely coloured oxalyldihydrazide-acetaldehyde complex (molar extinction coefficient 23,000 to 23,500). The methods are applicable on a routine basis to any biological material, in particular to serum, urine, or tissues, such as liver, brain, or kidney. In the case of urine a simple semi-quantitative screening method is also described. For quantitative work copper is liberated from serum by acid extraction and protein precipitation, and from urine and tissues by a rapid wet-ashing procedure. Recoveries of added copper from urine are quantitative. Precision is high although day-to-day control with standards and blanks is desirable. The methods are applied in an investigation of the diurnal variations in a normal man and in two cases of Wilson's disease.
mesh_terms: D001769:Blood; D001826:Body Fluids; D001921:Brain; D003300:Copper; D006527:Hepatolenticular Degeneration; D006801:Humans; D006834:Hydrazines; D007668:Kidney; D008297:Male; D008832:Microchemistry; D010070:Oxalates; D010507:Periodicity; D014556:Urine
chemical_list: D006834:Hydrazines; D010070:Oxalates; D003300:Copper; C014724:oxalic acid hydrazide
keywords: BLOOD; COPPER; HEPATOLENTICULAR DEGENERATION; HYDRAZINES; MICROCHEMISTRY; OXALATES; PERIODICITY; URINE