COTTON CLOSURE AS AN AERATION BARRIER IN SHAKEN FLASK FERMENTATIONS.
Oxygen transfer through a cotton plug follows Fick's law of diffusion. The apparent diffusion constant decreases as the density of cotton packing is increased. Formulas are derived which relate oxygen transfer to cotton-plug dimensions. Calculations indicate that the dimensions of a cotton plug can affect both the maximal oxygen uptake rate and the gas composition inside a shaken flask. In some situations, as with large flasks, the cotton closure can become the limiting factor to aeration.
D001410:Bacillus megaterium; D001431:Bacteriological Techniques; D002751:Chlortetracycline; D032561:Cotton Fiber; D004058:Diffusion; D004864:Equipment and Supplies; D005285:Fermentation; D005740:Gases; D003368:Gossypium; D010100:Oxygen; D012106:Research
D005740:Gases; D010100:Oxygen; D002751:Chlortetracycline
BACILLUS MEGATERIUM; BACTERIOLOGICAL TECHNICS; CHLORTETRACYCLINE; COTTON; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; FERMENTATION; GASES; OXYGEN