INDUCTION OF ACQUIRED RESISTANCE IN GUINEA PIGS WITH DEFATTED MYCOBACTERIUM TUBERCULOSIS VACCINES.
Smith, D. W. (University of Wisconsin, Madison), G. B. Fregnan, L. DeLaquerriere-Richardson, and E. Valdivia. Induction of acquired resistance in guinea pigs with defatted Mycobacterium tuberculosis vaccines. J. Bacteriol. 88:87-92. 1964.-Residues of mycobacteria exhaustively extracted with neutral organic solvents have been shown to produce a level of resistance against tuberculosis in guinea pigs comparable to that produced by BCG. Disruption of defatted tubercle bacilli in adjuvant eliminated the acid-fast staining property of the residue, but immunizing and sensitizing properties were retained in the fraction which sedimented at 44,000 x g. To demonstrate these activities with 50 mug of vaccine, it was necessary to use Bayol-Arlacel adjuvant. A similar quantity of vaccine given in saline was inactive. A defatted vaccine prepared from a virulent strain of Mycobacterium tuberculosis recently passed through a guinea pig was significantly more immunogenic than a similar vaccine prepared from H37Ra.
D000818:Animals; D001500:BCG Vaccine; D006168:Guinea Pigs; D009169:Mycobacterium tuberculosis; D012106:Research; D014376:Tuberculosis / Q000276:immunology*; D032581:Tuberculosis Vaccines; D014611:Vaccination; D014612:Vaccines
D001500:BCG Vaccine; D032581:Tuberculosis Vaccines; D014612:Vaccines
EXPERIMENTAL LAB STUDY; GUINEA PIGS; TUBERCULOSIS IMMUNITY; TUBERCULOSIS VACCINES