PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.
Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified diethylaminoethyl-cellulose columns. The material thus isolated appears to be electrophoretically and ultracentrifugally homogeneous. A molecular weight of 18,600 was calculated for the toxin.
D000992:Antitoxins; D001905:Botulinum Toxins; D001955:British Columbia; D002170:Canada; D002623:Chemistry Techniques, Analytical; D002845:Chromatography; D003014:Clostridium botulinum; D047970:Clostridium botulinum type E; D004586:Electrophoresis; D008970:Molecular Weight; D012106:Research; D014118:Toxins, Biological; D014461:Ultracentrifugation
D000992:Antitoxins; D014118:Toxins, Biological; D001905:Botulinum Toxins; C096326:botulinum toxin type E
CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY; CLOSTRIDIUM BOTULINUM; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; MOLECULAR WEIGHT; TOXINS AND ANTITOXINS; ULTRACENTRIFUGATION