RECONSTITUTION OF ANTIPHAGE ANTIBODIES FROM L AND H POLYPEPTIDE CHAINS AND THE FORMATION OF INTERSPECIES MOLECULAR HYBRIDS.
Reconstitution of phage-neutralizing activity was observed after admixture of separated L and H polypeptide chains of purified antibodies obtained from single sheep or guinea pigs and directed against f1 and f2 phages. Hybrid mixtures of complementary chains of antiphage antibodies and gamma-globulins of the same animal showed less activity than the homologous mixtures of chains from antibodies of the same specificity. 7S interspecies molecular hybrids could be formed between L or H chains of sheep gamma-globulins and the complementary chains of guinea pig gamma-globulins. In contrast to the results obtained within a single animal species, mixtures of L or H chains of antiphage antibodies from one species with the complementary chains of antibodies to the same phage from the other species showed slight or negligible potentiation of neutralizing activity.
D000818:Animals; D000906:Antibodies; D001435:Bacteriophages; D004586:Electrophoresis; D006168:Guinea Pigs; D006824:Hybridization, Genetic; D009500:Neutralization Tests; D010455:Peptides; D012106:Research; D012756:Sheep; D014461:Ultracentrifugation; D005719:gamma-Globulins
D000906:Antibodies; D010455:Peptides; D005719:gamma-Globulins
ANTIBODIES; BACTERIOPHAGE; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN; GUINEA PIGS; HYBRIDIZATION; NEUTRALIZATION TESTS; PEPTIDES; SHEEP; ULTRACENTRIFUGATION