EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY.
Anti-p-azophenyl-beta-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, a 10S gamma(1) globulin, and three antigenically distinct 7S gamma-globulins. The specific hapten-binding activity of the 7S beta(2A)-antibody and of the 10S gamma(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S gamma(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins.
D000818:Animals; D000906:Antibodies; D000941:Antigens; D001609:Beta-Globulins; D002845:Chromatography; D006027:Glycosides; D006241:Haptens; D006433:Hemocyanins; D006736:Horses; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007785:Lactose; D012106:Research; D012709:Serum Albumin; D014461:Ultracentrifugation; D005719:gamma-Globulins
D000906:Antibodies; D000941:Antigens; D001609:Beta-Globulins; D006027:Glycosides; D006241:Haptens; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012709:Serum Albumin; D005719:gamma-Globulins; C008245:lactosides; D006433:Hemocyanins; D007785:Lactose
ANTIBODIES; BETA GLOBULIN; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN, 7S; HAPTENS; HORSES; IMMUNOELECTROPHORESIS; LACTOSE; SERUM ALBUMIN; ULTRACENTRIFUGATION