REPLICATION OF THE RNA OF BACTERIOPHAGE R17.
Escherichia coli cells were irradiated with ultraviolet light to stop ribosomal RNA synthesis. After infection of such cells with the single-stranded RNA phage R17, the RNA most rapidly labeled by a pulse of tritiated uridine sedimented in a broad band in the 16S region of sucrose gradients. The effect of ribonuclease on this material and its behavior during a "chase" period in nonradioactive medium suggest that it consists of a core of double-stranded RNA, one strand of which-the viral strand-is continually displaced by a growing strand forming single-stranded tails and ultimately free 27S viral RNA.
D003090:Coliphages; D004261:DNA Replication; D004926:Escherichia coli; D008660:Metabolism; D009705:Nucleosides; D010600:Pharmacology; D012313:RNA; D012330:RNA, Double-Stranded; D012335:RNA, Ribosomal; D012367:RNA, Viral; D012106:Research; D012260:Ribonucleases; D012270:Ribosomes; D014316:Tritium; D014466:Ultraviolet Rays; D014529:Uridine
D009705:Nucleosides; D012330:RNA, Double-Stranded; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D012313:RNA; D012260:Ribonucleases; D014529:Uridine
COLIPHAGES; EXPERIMENTAL LAB STUDY; METABOLISM; NUCLEOSIDES; PHARMACOLOGY; RIBONUCLEASE; RNA, VIRAL; TRITIUM; ULTRAVIOLET RAYS