title: BONE CELLS: BIOCHEMICAL AND BIOLOGICAL STUDIES AFTER ENZYMATIC ISOLATION.
abstract: Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.
mesh_terms: D001842:Bone and Bones; D050260:Carbohydrate Metabolism; D003585:Cell Biology; D010450:Endopeptidases; D005947:Glucose; D051381:Rats; D012106:Research; D012886:Skull; D046509:Tissue Culture Techniques
chemical_list: D010450:Endopeptidases; D005947:Glucose
keywords: BONE AND BONES; CARBOHYDRATE METABOLISM; CYTOLOGY; EXPERIMENTAL LAB STUDY; GLUCOSE; PEPTIDE PEPTIDOHYDROLASES; RATS