DELAYED APPEARANCE OF LABELED PROTEIN IN ISOLATED NERVE ENDINGS AND AXOPLASMIC FLOW.
Subcellular fractions of mouse brain were prepared by differential and sucrose density-gradient centrifugation at intervals up to 130 hours after intracerebral injection of C(14)- labeled leucine. The specific activity of the nerve ending fraction continued to rise during this period. Between 2 and 130 hours after injection there was a fivefold rise in the specific activity of the soluble protein prepared by lysing the nerve ending particles with water while the specific activity of the soluble protein from the whole homogenate concomitantly fell almost by a factor of three. The data are interpreted as being consistent with the appearance of protein in nerve endings by axoplasmic flow.
D000818:Animals; D001370:Axonal Transport; D001921:Brain; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D007930:Leucine; D051379:Mice; D009411:Nerve Endings; D009448:Neurochemistry; D011506:Proteins / Q000378:metabolism*; D012106:Research
D002247:Carbon Isotopes; D011506:Proteins; D007930:Leucine
BRAIN CHEMISTRY; CARBON ISOTOPES; EXPERIMENTAL LAB STUDY; LEUCINE; MICE; NERVE ENDINGS; NEUROCHEMISTRY; PROTEIN METABOLISM