title: CULTURE OF INSECT SALIVARY GLANDS IN A CHEMICALLY DEFINED MEDIUM.
abstract: A method for culturing the salivary glands of Sciara coprophila in a chemically defined medium has been developed. During a 24-hour period, the polytene chromosomes from six larval stages undergo normal developmental mental changes, including puff formation, puff condensation, and DNA synthesis.
mesh_terms: D000818:Animals; D002875:Chromosomes; D003470:Culture Media; D004261:DNA Replication; D004175:Diptera; D007313:Insecta; D007814:Larva; D012106:Research; D012469:Salivary Glands; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media
keywords: CULTURE MEDIA; EXPERIMENTAL LAB STUDY; INSECTS; SALIVARY GLANDS; TISSUE CULTURE