title: BIOTIN DEFICIENCY AND THE FATTY ACIDS OF CERTAIN BIOTIN-REQUIRING BACTERIA.
abstract: Croom, Jo A. (North Carolina State of the University of North Carolina, Raleigh), J. J. McNeill, and S. B. Tove. Biotin deficiency and the fatty acids of certain biotin-requiring bacteria. J. Bacteriol. 88:389-394. 1964.-The major fatty acids of Lactobacillus plantarum were identified as myristic, palmitic, palmitoleic, stearic, cis-vaccenic, and lactobacillic. Small amounts of a C(14)-monoenoic acid were also found. The major acids of a biotin-requiring mutant of Escherichia coli were lauric, myristic, palmitic, palmitoleic, methylene-hexadecanoic, octadecenoic, and methylene-octadecanoic. The C(16)-monoenoic acid fraction of E. coli also contained small amounts of 7,8-hexadecenoate. The C(18)-monoenoic acid fraction contained 9,10-octadecenoic acid (oleic), with small amounts of 7,8-octadecenoic. Two other components, which had properties similar to tridecanoic and pentadecanoic acids, were also found. Biotin deficiency in L. plantarum decreased the relative amounts of cis-vaccenic and lactobacillic acids, and increased palmitic acid. In E. coli there was no change in the proportion of palmitic acid, whereas there were increases in monoenoic acids and a decreased level of methylene-hexadecanoic acid. In L. plantarum, palmitic and lactobacillic acids increased with age, and cis-vaccenic decreased sharply. In biotin deficiency, there was a decrease of about 20% in total lipid of L. plantarum.
mesh_terms: D001710:Biotin; D028921:Biotinidase Deficiency; D004926:Escherichia coli; D005227:Fatty Acids / Q000378:metabolism*; D007778:Lactobacillus; D048191:Lactiplantibacillus plantarum; D009829:Oleic Acids; D010600:Pharmacology
chemical_list: D005227:Fatty Acids; D009829:Oleic Acids; C028528:methylene hexadecanoic acid; D001710:Biotin; C117025:pentadecanoic acid; C046568:10-octadecenoic acid; C028716:phytomonic acid
keywords: BIOTIN; ESCHERICHIA COLI; FATTY ACID METABOLISM; FATTY ACIDS; LACTOBACILLUS; PHARMACOLOGY