PARTIAL PURIFICATION OF THE EXTRACELLULAR HEMOLYSIN OF PSEUDOMONAS AERUGINOSA.
Berk, Richard S. (Wayne State University, College of Medicine, Detroit, Mich.). Partial purification of the extracellular hemolysin of Pseudomonas aeruginosa. J. Bacteriol. 88:559-565. 1964.-Through a series of chemical fractionation steps, the extracellular hemolysin of Pseudomonas aeruginosa was purified 126-fold with a recovery of 49%. Hemolytic activity of crude preparations was irreversibly lost upon contact with anionic exchange materials such as diethylaminoethyl Sephadex or ECTEOLA-Cellulose, but traveled with the solvent front during passage through Sephadex G-200 and carboxymethyl Sephadex. The hemolysin was soluble in water and ethanol, and was partially extractable with ether, but not with trichlorotrifluoroethane (Freon). Although normal serum and serum albumin blocked hemolytic activity, it was unaffected by trypsin, deoxyribonuclease, or ribonuclease. Partially purified hemolysin was studied in vivo, but did not exert dermonecrotic activity in mice or rabbits in the concentrations tested. Although preparations were toxic to mice, lethality appeared to be more a reflection of the nonhemolytic protein content of the preparations rather than of hemolytic activity.
D000818:Animals; D001769:Blood; D002463:Cell Membrane Permeability; D002482:Cellulose / Q000031:analogs & derivatives; D002712:Chlorides; D004247:DNA; D003851:Deoxyribonucleases; D006460:Hemolysin Proteins; D007475:Ion Exchange Resins; D051379:Mice; D010600:Pharmacology; D011506:Proteins; D011550:Pseudomonas aeruginosa; D011817:Rabbits; D012106:Research; D012260:Ribonucleases; D012709:Serum Albumin; D013053:Spectrophotometry; D014116:Toxicology; D014357:Trypsin
D002712:Chlorides; D006460:Hemolysin Proteins; D007475:Ion Exchange Resins; D011506:Proteins; D012709:Serum Albumin; D002482:Cellulose; D004247:DNA; C020311:epichlorohydrin triethanolamine cellulose; D003851:Deoxyribonucleases; D012260:Ribonucleases; D014357:Trypsin
BLOOD; CELL MEMBRANE PERMEABILITY; CHLORIDES; DEOXYRIBONUCLEASE; EXPERIMENTAL LAB STUDY; HEMOLYSINS; ION EXCHANGE RESINS; MICE; PHARMACOLOGY; PSEUDOMONAS AERUGINOSA; RABBITS; RIBONUCLEASE; SERUM ALBUMIN; SPECTROPHOTOMETRY; TOXICOLOGIC REPORT; TRYPSIN