TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY.
Karakawa, Walter W. (Connecticut State Department of Health, Hartford), Earle K. Borman, and Clarence R. McFarland. Typing of group A streptococci by immunofluorescence. I. Preparation and properties of type 1 fluorescein-labeled antibody. J. Bacteriol. 87:1377-1382. 1964.-Unadsorbed, fluorescein-labeled globulins derived from rabbits immunized with acid-extracted M-protein of type 1 streptococci (plus adjuvant) were found to have high fluorescent-antibody (FA) staining titers and to be considerably more type-specific than were similar preparations derived from whole-cell immunization. Appropriate adsorption rendered the anti-M reagent entirely type-specific without appreciable loss of titer; whole-cell reagent was appreciably weakened in FA titer by comparable adsorption. Type-specificity was confirmed by parallel bactericidal, long-chain, and precipitin studies. Removal of reactivity by adsorption with homologous M protein was complete, confirming that the FA reaction was truly a manifestation of an M anti-M protein system. The data indicate that the development of FA reagents specific for the streptococcal types is feasible.
D000818:Animals; D001431:Bacteriological Techniques; D019793:Fluorescein; D005455:Fluorescent Antibody Technique; D011817:Rabbits; D012106:Research; D013194:Staining and Labeling; D013291:Streptococcus; D013297:Streptococcus pyogenes
D019793:Fluorescein
BACTERIOLOGICAL TECHNICS; EXPERIMENTAL LAB STUDY; FLUORESCENT ANTIBODY TECHNIC; STREPTOCOCCUS