title: THE EFFECT OF PROTEOLYTIC ENZYMES ON E. COLI PHAGES AND ON NATIVE PROTEINS.
abstract: Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T(2) phage is slowly inactivated by high concentrations of (alpha-, beta-, gamma-, or Delta-chymotrypsin, but not by trypsin or ficin. P(1) phage is slowly inactivated by alpha-, beta-, or gamma-chymotrypsin, or ficin, more rapidly by Delta-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystalline serum albumin, E. coli nucleoprotein, and yeast nucleoprotein are hydrolyzed slowly by alpha-chymotrypsin. Yeast nucleoprotein, like P(1) phage, is hydrolyzed more rapidly by Delta-chymotrypsin than by alpha-chymotrypsin, but not by trypsin or ficin. Neither phages nor native proteins were attacked by papain, carboxypeptidase, deoxyribonuclease, or ribonuclease.
mesh_terms: D001435:Bacteriophages; D002268:Carboxypeptidases; D002918:Chymotrypsin; D003090:Coliphages; D004247:DNA; D003851:Deoxyribonucleases; D010450:Endopeptidases; D004926:Escherichia coli; D007700:Kinetics; D009698:Nucleoproteins; D010047:Ovalbumin; D010206:Papain; D010447:Peptide Hydrolases; D011506:Proteins; D012106:Research; D012260:Ribonucleases; D012709:Serum Albumin; D014357:Trypsin; D015003:Yeasts
chemical_list: D009698:Nucleoproteins; D011506:Proteins; D012709:Serum Albumin; D010047:Ovalbumin; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D002268:Carboxypeptidases; D010450:Endopeptidases; D010447:Peptide Hydrolases; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; C117605:gamma-chymotrypsin; D014357:Trypsin; D010206:Papain
keywords: CARBOXYPEPTIDASES; CHYMOTRYPSIN; COLIPHAGES; DEOXYRIBONUCLEASE; EXPERIMENTAL LAB STUDY; KINETICS; NUCLEOPROTEINS; OVALBUMIN; PAPAIN; PEPTIDE PEPTIDOHYDROLASES; RIBONUCLEASE; SERUM ALBUMIN; TRYPSIN; YEASTS