POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO.
Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.
D002247:Carbon Isotopes; D004926:Escherichia coli; D066298:In Vitro Techniques; D010455:Peptides; D010649:Phenylalanine; D011132:Polyribosomes; D011506:Proteins / Q000378:metabolism*; D012313:RNA; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012106:Research; D012270:Ribosomes; D012440:Saccharomyces; D012441:Saccharomyces cerevisiae
D002247:Carbon Isotopes; D010455:Peptides; D011506:Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D010649:Phenylalanine; D012313:RNA
CARBON ISOTOPES; EXPERIMENTAL LAB STUDY; PHENYLALANINE; PROTEIN METABOLISM; RIBOSOMES; RNA, MESSENGER; SACCHAROMYCES