title: PRODUCTION OF PROTOPLASTS IN AN OSMOTIC MUTANT OF NEUROSPORA CRASSA WITHOUT ADDED ENZYME.
abstract: Hamilton, James G. (Tulane University School of Medicine, New Orleans, La.), and Janet Calvet. Production of protoplasts in an osmotic mutant of Neurospora crassa without added enzyme. J. Bacteriol. 88:1084-1086. 1964.-An osmotic mutant of Neurospora crassa was grown in minimal medium containing increasing concentrations of sorbose. Protoplasts were produced in the higher concentrations by inhibition of cell-wall synthesis. Similar molar concentrations of fructose, glucose, and sucrose also produced protoplasts, suggesting a relationship between inhibition of cell-wall synthesis and osmotic pressure. Increased sugar concentration led to decreased growth.
mesh_terms: D003470:Culture Media; D005632:Fructose; D005947:Glucose; D008660:Metabolism; D009154:Mutation; D009491:Neurospora; D009492:Neurospora crassa; D009995:Osmosis; D011523:Protoplasts; D012106:Research; D013013:Sorbose; D013395:Sucrose
chemical_list: D003470:Culture Media; D005632:Fructose; D013395:Sucrose; D005947:Glucose; D013013:Sorbose
keywords: CULTURE MEDIA; EXPERIMENTAL LAB STUDY; FRUCTOSE; GLUCOSE; METABOLISM; MUTATION; NEUROSPORA; PROTOPLASTS; SORBOSE; SUCROSE