RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN.
Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects.
D000614:Aminocaproates; D015119:Aminocaproic Acid; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000941:Antigens; D003956:Dialysis; D004140:Dinitrophenols; D006241:Haptens; D007120:Immunochemistry; D007456:Iodine Isotopes; D010455:Peptides; D011995:Recombination, Genetic; D006435:Renal Dialysis; D012106:Research; D005719:gamma-Globulins
D000614:Aminocaproates; D000906:Antibodies; D000941:Antigens; D004140:Dinitrophenols; D006241:Haptens; D007456:Iodine Isotopes; D010455:Peptides; D005719:gamma-Globulins; D015119:Aminocaproic Acid
AMINOCAPROIC ACID; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; DIALYSIS; DINITROPHENOLS; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN; HAPTENS; IMMUNOCHEMISTRY; IODINE ISOTOPES; PEPTIDES