ESTIMATION OF SERUM HAEMOGLOBIN-BINDING CAPACITY (HAPTOGLOBIN) ON SEPHADEX G.100.
A method is described for the estimation of serum haptoglobin as ;haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide range of values.
D002845:Chromatography; D003911:Dextrans; D006242:Haptoglobins; D006454:Hemoglobins; D007122:Immunoelectrophoresis; D011485:Protein Binding
D003911:Dextrans; D006242:Haptoglobins; D006454:Hemoglobins; C035492:haptoglobin-hemoglobin complex; C025614:sephadex
CHROMATOGRAPHY; DEXTRAN; HAPTOGLOBINS; HEMOGLOBIN; IMMUNOELECTROPHORESIS