title: MITOMYCIN C: EFFECT ON RIBOSOMES OF ESCHERICHIA COLI.
abstract: Sucrose-gradient analyses of cell-free extracts of Escherichia coli B show that the sedimentation patterns are markedly affected by exposure of the cells to mitomycin C; 50S subunits are progressively degraded in the cells after 30 minutes exposure. The 30S subunits, although affected, are less sensitive to the antibiotic than the 50S subunits. Incorporation of uracil-2-C(14) into the ribosomal particles by the treated cells is markedly reduced by exposure.
mesh_terms: D004926:Escherichia coli; D016685:Mitomycin; D008937:Mitomycins; D010600:Pharmacology; D011506:Proteins / Q000378:metabolism*; D012106:Research; D012270:Ribosomes
chemical_list: D008937:Mitomycins; D011506:Proteins; D016685:Mitomycin
keywords: ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; MITOMYCIN; PHARMACOLOGY; PROTEIN METABOLISM; RIBOSOMES