title: POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO.
abstract: Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.
mesh_terms: D002247:Carbon Isotopes; D004926:Escherichia coli; D066298:In Vitro Techniques; D010455:Peptides; D010649:Phenylalanine; D011132:Polyribosomes; D011506:Proteins / Q000378:metabolism*; D012313:RNA; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012106:Research; D012270:Ribosomes; D012440:Saccharomyces; D012441:Saccharomyces cerevisiae
chemical_list: D002247:Carbon Isotopes; D010455:Peptides; D011506:Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D010649:Phenylalanine; D012313:RNA
keywords: CARBON ISOTOPES; EXPERIMENTAL LAB STUDY; PHENYLALANINE; PROTEIN METABOLISM; RIBOSOMES; RNA, MESSENGER; SACCHAROMYCES