QUANTITATIVE SEPARATION OF RADIOACTIVE STEROLS AND BILE ACIDS IN HUMAN FAECES.
A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination between the bile-acid and sterol fractions is between 0.7 and 3.8%. The method gives better results than one based on separation of the faecal steroids into saponifiable and unsaponifiable material.
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D002784:Cholesterol; D002845:Chromatography; D005243:Feces; D006801:Humans; D012492:Salts; D013256:Steroids; D013261:Sterols; D014316:Tritium
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D012492:Salts; D013256:Steroids; D013261:Sterols; D014316:Tritium; D002784:Cholesterol
BILE ACIDS AND SALTS; CARBON ISOTOPES; CHOLESTEROL; CHROMATOGRAPHY; FECES; TRITIUM