title: SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE.
abstract: Suzuki, T. (Children's Hospital, Buffalo, N.Y.), E. A. Gorzynski, and E. Neter. Separation by ethanol of common and somatic antigens of Enterobacteriaceae. J. Bacteriol. 88:1240-1243. 1964.-Ethanol in a concentration of 85% permits separation from crude culture supernatant fluids of the common enterobacterial antigen and the O antigen, the former being ethanol-soluble, the latter being ethanol-insoluble. The evidence was obtained in hemagglutination, hemolysis, and hemagglutination-inhibition tests. In contrast to the crude antigens obtained from enteric bacteria other than Escherichia coli O14, the ethanol-soluble fraction is immunogenic in the rabbit upon intravenous injection, and antibodies against the common antigen are produced in relatively high titers. Aqueous mixtures of ethanol-soluble and -insoluble fractions engender antibodies against the common antigen in titers significantly lower than those stimulated by the soluble fraction alone. Ethanol-treated whole antigen fails to stimulate antibody formation against this antigen. These results suggest the presence of an inhibitor in the ethanol-insoluble fraction.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D000942:Antigens, Bacterial; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae; D004926:Escherichia coli; D000431:Ethanol; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006461:Hemolysis; D006801:Humans; D011817:Rabbits; D012106:Research; D012486:Salmonella typhimurium
chemical_list: D000941:Antigens; D000942:Antigens, Bacterial; C017286:enterobacterial common antigen; D000431:Ethanol
keywords: ALCOHOL, ETHYL; ANTIBODY FORMATION; ANTIGENS; BACTERIOLOGICAL TECHNICS; ENTEROBACTERIACEAE; EXPERIMENTAL LAB STUDY; HEMAGGLUTINATION; HEMAGGLUTINATION INHIBITION TESTS; HEMOLYSIS; RABBITS