PURIFICATION AND PROPERTIES OF STAPHYLOCOCCAL BETA-HEMOLYSIN. I. PRODUCTION OF BETA-HEMOLYSIN.
Haque, Riaz-ul (Ohio State University, Columbus), and Jack N. Baldwin. Purification and properties of staphylococcal beta-hemolysin. I. Production of beta-hemolysin. J. Bacteriol. 88:1304-1309. 1964.-Highest activity of beta-hemolysin was observed when buffered saline (pH 7.0) containing 0.001 m magnesium sulfate was used as a diluent, and the tubes were incubated at 37 C for 80 min and then refrigerated for 30 min. Either Heart Infusion semisolid agar or a dialysate of Heart Infusion containing 0.3% agar was suitable for the production of large quantities of beta-hemolysin. The concentration of beta-hemolysin in semisolid and broth cultures was greatest after incubation for 24 hr. Continued incubation resulted in a loss of active hemolysin in broth cultures but not in semisolid agar cultures. Incubation in atmospheres containing 20% carbon dioxide greatly enhanced the production of beta-hemolysin. The presence of fermentable sugars inhibited the production of beta-hemolysin. Highest yields of beta-hemolysin were obtained when the initial pH of the medium was 5.5 to 5.8.
D000362:Agar; D000438:Alcohols; D002118:Calcium; D002241:Carbohydrates; D002623:Chemistry Techniques, Analytical; D002712:Chlorides; D003470:Culture Media; D005285:Fermentation; D006460:Hemolysin Proteins; D006601:Hexoses; D007501:Iron; D008274:Magnesium; D008278:Magnesium Sulfate; D012106:Research; D013210:Staphylococcus; D013431:Sulfates
D000438:Alcohols; D002241:Carbohydrates; D002712:Chlorides; D003470:Culture Media; D006460:Hemolysin Proteins; D006601:Hexoses; D013431:Sulfates; D008278:Magnesium Sulfate; D000362:Agar; D007501:Iron; D008274:Magnesium; D002118:Calcium
ALCOHOLS; CALCIUM; CARBOHYDRATES; CHEMISTRY, ANALYTICAL; CHLORIDES; EXPERIMENTAL LAB STUDY; HEMOLYSINS; IRON; MAGNESIUM; MAGNESIUM SULFATE; STAPHYLOCOCCUS; SULFATES