PROPERTIES OF A LYSOZYME-DISSOCIATED ENDOTOXIC FRACTION FROM ESCHERICHIA COLI.
Mergenhagen, Stephan E. (National Institute of Dental Research, Bethesda, Md.), and George R. Martin. Properties of a lysozyme-dissociated endotoxic fraction from Escherichia coli. J. Bacteriol. 88:1169-1174. 1964.-Treatment of a phenol-water preparation of C(14)-labeled Escherichia coli O91-H21 endotoxin of low solubility with lysozyme at pH 5.0 or 8.0 effected a dissociation of the preparation. Such products of dissociation were equally distributed in the chloroform and water phases after extraction. beta-Glucosidase, but not beta-galactosidase, significantly dissociated this endotoxin also. Concomitant with dissociation, recoverable endotoxin after lysozyme treatment had a reduced content of bound lipid, and dissolved easily in aqueous media to yield a clear solution. Examination of lysozyme-treated endotoxin in an analytical ultracentrifuge revealed that it sedimented as a single major boundary with a sedimentation coefficient of 13.3. Lysozyme-treated endotoxin was more potent than was the conventional endotoxin as evidenced by lethal activity in rabbits and pertussis-sensitized mice. Agar-gel diffusion analysis indicated that the higher molecular weight component associated with conventional endotoxin was dissociated by lysozyme treatment. In immunoelectrophoresis, lysozyme-treated endotoxin was observed as a single sharp band of precipitation which migrated toward the cathode.
D000818:Animals; D004731:Endotoxins; D004926:Escherichia coli; D005959:Glucosidases; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D009113:Muramidase; D011817:Rabbits; D012106:Research; D014116:Toxicology; D014461:Ultracentrifugation; D001616:beta-Galactosidase
D004731:Endotoxins; D005959:Glucosidases; D009113:Muramidase; D001616:beta-Galactosidase
ENDOTOXINS; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; GEL DIFFUSION TESTS; GLUCOSIDASE; IMMUNOELECTROPHORESIS; MICE; MURAMIDASE; RABBITS; TOXICOLOGIC REPORT; ULTRACENTRIFUGATION