title: BACTERIAL METABOLISM OF 2-METHYLALANINE.
abstract: Aaslestad, H. G. (Louisiana State University, Baton Rouge), and A. D. Larson. Bacterial metabolism of 2-methylalanine. J. Bacteriol. 88:1296-1303. 1964.-A bacterium isolated from soil was found to oxidize 2-methylalanine to 65% of the theoretical value by an adaptive enzyme system. Manometric experiments with whole cells indicated that acetone but not isopropylamine was an intermediate. Cellular extracts produced carbon dioxide from 2-methylalanine when pyruvate was added to the reaction mixture. Dialysis stimulated the 2-methylalanine metabolizing system, providing pyridoxal phosphate and pyruvate were supplied. A study of the stoichiometry of this pyridoxal phosphate-dependent reaction indicated that acetone and carbon dioxide were principal products. The formation of carbon dioxide from 2-methylalanine seemed dependent upon transfer of the amino group to pyridoxal phosphate. Other 2-methyl amino acids were found to be decarboxylated by this system, thus indicating a lack of absolute specificity.
mesh_terms: D000096:Acetone; D000409:Alanine; D000588:Amines; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids; D002245:Carbon Dioxide; D008365:Manometry; D011732:Pyridoxal Phosphate; D011773:Pyruvates; D006435:Renal Dialysis; D012106:Research; D012988:Soil Microbiology
chemical_list: D000588:Amines; D000596:Amino Acids; D000621:Aminoisobutyric Acids; D011773:Pyruvates; D000096:Acetone; D002245:Carbon Dioxide; C100049:2-aminoisobutyric acid; D011732:Pyridoxal Phosphate; D000409:Alanine
keywords: ACETONE; ALANINE; AMINES; AMINO ACID METABOLISM; EXPERIMENTAL LAB STUDY; MANOMETRY; PYRIDOXAL PHOSPHATE; PYRUVATES; SOIL MICROBIOLOGY