CONTACT-INDUCED CYTOTOXICITY BY LYMPHOID CELLS CONTAINING FOREIGN ISOANTIGENS.
In tissue culture, immune lymph node cells containing foreign histocompatibility antigens of the H-2 type exert marked cytotoxic effects on tumor cells incompatible with the H-2 antigen. An equally pronounced effect is obtained when normal allogeneic and semi-isologous lymphoid cells of F(1) hybrids are caused to aggregate around the target tumor cells by treating the cultures with either heat-inactivated rabbit antiserum to mouse cells or phytohemagglutinin. Isologous lymph node cells have no effect. Thus, aggregation of lymphoid cells and target cells is a necessary but insufficient requirement for cytotoxicity in vitro; in addition, close contact must be established between histoincompatible cells.
D000818:Animals; D000941:Antigens; D003470:Culture Media; D006183:H-2 Antigens; D007106:Immune Sera; D007114:Immunization; D007159:Immunologic Tests; D007519:Isoantigens; D008198:Lymph Nodes; D008214:Lymphocytes; D008748:Methylcholanthrene; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000276:immunology*; D037121:Plant Lectins; D011817:Rabbits; D012106:Research; D012509:Sarcoma; D012513:Sarcoma, Experimental; D046509:Tissue Culture Techniques; D014116:Toxicology; D014611:Vaccination
D000941:Antigens; D003470:Culture Media; D006183:H-2 Antigens; D007106:Immune Sera; D007519:Isoantigens; D037121:Plant Lectins; D008748:Methylcholanthrene
ANTIGENS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; IMMUNE SERUMS; IMMUNIZATION; LYMPH NODES; METHYLCHOLANTHRENE; MICE; NEOPLASM IMMUNOLOGY; NEOPLASM TRANSPLANTATION; PLANT AGGLUTININS; RABBITS; SARCOMA, EXPERIMENTAL; TISSUE CULTURE; TOXICOLOGIC REPORT