title: AMINO GROUP FORMATION AND GLUTAMATE SYNTHESIS IN STREPTOCOCCUS BOVIS.
abstract: Burchall, J. J. (University of Illinois, Urbana), R. A. Niederman, and M. J. Wolin. Amino group formation and glutamate synthesis in Streptococcus bovis. J. Bacteriol. 88:1038-1044. 1964.-Extracts of Streptococcus bovis grown on NH(4) (+) as a nitrogen source contain a nicotinamide adenine dinucleotide phosphate (NADP)-linked glutamic dehydrogenase and are devoid of alanine dehydrogenase, other amino acid dehydrohygenases, and aspartase. A potential source of reduced nicotinamide adenine dinucleotide phosphate for glutamate synthesis is a NADP and nicotinamide adenine dinucleotide (NAD)-linked glyceraldehyde-3-phosphate dehydrogenase present in the extracts. Experiments with C(14)-labeled glucose and NaHCO(3) indicate that the glutamate carbon skeleton is synthesized by a tricarboxylic acid pathway. The synthesis of the carbon skeleton of glutamate is repressed when glutamate or casein hydrolysate supplement the NH(4) (+)-containing growth medium. Repression of glutamic dehydrogenase and a NAD-linked isocitric dehydrogenase occurs only when complex nitrogen sources, but not when free amino acids, are added to the growth medium.
mesh_terms: D000596:Amino Acids; D001216:Asparagine; D001224:Aspartic Acid; D002244:Carbon; D002364:Caseins; D003470:Culture Media; D005969:Glutamate Dehydrogenase; D005971:Glutamates; D018698:Glutamic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007521:Isocitrate Dehydrogenase; D008660:Metabolism; D009243:NAD; D009249:NADP; D009584:Nitrogen; D009711:Nucleotides; D012106:Research; D013291:Streptococcus; D016985:Streptococcus bovis
chemical_list: D000596:Amino Acids; D002364:Caseins; D003470:Culture Media; D005971:Glutamates; D009711:Nucleotides; D009243:NAD; D001224:Aspartic Acid; D018698:Glutamic Acid; D009249:NADP; C475729:casein hydrolysate; D001216:Asparagine; D002244:Carbon; D007521:Isocitrate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005969:Glutamate Dehydrogenase; D009584:Nitrogen
keywords: ASPARAGINE; ASPARTIC ACID; CASEIN; EXPERIMENTAL LAB STUDY; GLUTAMATE DEHYDROGENASE; GLUTAMATES; GLYCERALDEHYDEPHOSPHATE DEHYDROGENASE; ISOCITRATE DEHYDROGENASE; METABOLISM; NADP; NUCLEOTIDES; STREPTOCOCCUS