ELECTRON MICROSCOPY OF A FISSION YEAST, SCHIZOSACCHAROMYCES POMBE.
Maclean, Norman (University of Edinburgh, Edinburgh, Scotland). Electron microscopy of a fission yeast, Schizosaccharomyces pombe. J. Bacteriol. 88:1459-1466. 1964.-The structure of the fission yeast, Schizosaccharomyces pombe, was studied electron microscopically, with potassium permanganate and osmium tetroxide as fixatives. The cell was found to be bounded by a cell wall, 1,000 to 2,000 A thick, and a cell membrane. A layer of material was found between the cell membrane and the wall. The central nucleus, 2 to 3 mu in diameter, was bounded by a nuclear membrane, seen in some pictures to be double. Osmium tetroxide fixation revealed a granular body within the nucleus, identified as a nucleolus. Cytoplasmic structures included numerous vacuoles (probably normally containing lipid), a number of membranes and vesicles (which may represent a poorly organized mitochondrial system), and numerous granules (probably representing ribosomes).
D002462:Cell Membrane; D002466:Cell Nucleolus; D002467:Cell Nucleus; D002473:Cell Wall; D003593:Cytoplasm; D003594:Cytoplasmic Granules; D004583:Electrons; D006651:Histocytochemistry; D008055:Lipids; D008853:Microscopy; D008854:Microscopy, Electron; D008928:Mitochondria; D012106:Research; D012270:Ribosomes; D012568:Schizosaccharomyces; D012606:Scotland; D015003:Yeasts
D008055:Lipids
CELL MEMBRANE; CELL NUCLEUS; CYTOPLASM; EXPERIMENTAL LAB STUDY; HISTOCYTOCHEMISTRY; LIPIDS; MICROSCOPY, ELECTRON; MITOCHONDRIA; RIBOSOMES; YEASTS