FIBRINOGEN PRECIPITATION BY STREPTOCOCCAL M PROTEIN. I. IDENTITY OF THE REACTANTS, AND STOICHIOMETRY OF THE REACTION.
Evidence confirming the identity of fibrinogen-precipitating factor and streptococcal M protein is provided by the demonstration of bactericidal, mouse protective, and long chain-producing antibodies in the sera of rabbits immunized with washed M-fibrinogen precipitates. Two precipitin lines were observed in immunoelectrophoresis of human plasma vs. rabbit anti-M-fibrinogen antiserum; no precipitin reactions were observed between human serum and rabbit anti-M-fibrinogen antiserum. The reaction between M protein and fibrinogen was stoichiometric; a constant equimolar ratio of reactants was observed in precipitates formed with a wide range of M protein concentrations.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D011232:Chemical Precipitation; D005340:Fibrinogen; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007122:Immunoelectrophoresis; D007456:Iodine Isotopes; D051379:Mice; D011233:Precipitin Tests; D011817:Rabbits; D011874:Radiometry; D012106:Research; D013291:Streptococcus; D014611:Vaccination
D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D007456:Iodine Isotopes; C014689:streptococcal M protein; D005340:Fibrinogen
BACTERIAL PROTEINS; EXPERIMENTAL LAB STUDY; FIBRINOGEN; IMMUNIZATION; IMMUNOELECTROPHORESIS; IODINE ISOTOPES; PRECIPITATION; PRECIPITIN TESTS; RABBITS; RADIOMETRY; STREPTOCOCCUS