MODIFICATION OF HOST RESPONSES TO BACTERIAL ENDOTOXINS. II. PASSIVE TRANSFER OF IMMUNIY TO BACTERIAL ENDOTOXIN WITH FRACTIONS CONTAINING 19S ANTIBODIES.
Serum from rabbits rendered tolerant or immune to 100 MPD-3/kg of endotoxin when passively transferred to normal rabbits gave partial tolerance to the standard dose of endotoxin. The same serum was fractionated by DEAE chromatography into 4 major fractions. Immunoelectrophoretic analysis indicated that the 7S gamma(2)-and the 19S gamma(1)-immunoglobulins were separated into two distinct fractions. Of the four fractions tested, only fraction IV containing 19S gamma(1)-immunoglobulm conferred complete pyrogenic tolerance to 100 MPD-3/kg of endotoxin. Additional fractionation of DEAE fraction IV by exclusion chromatography on sephadex G-200 gave 3 fractions. Of these only the first, containing 19S gamma(1)-immunoglobulin conferred complete pyrogenic and lethal tolerance to normal rabbits. There was no correlation between the quantity of O-specific antibodies and the ability to transfer tolerance. It is concluded that endotoxin tolerance involves a classical immune mechanism which includes both 19S gamma(1)-immunoglobulin specific for toxophore groups common to many endotoxins and a normally functioning RES. To avoid confusion with immunologic tolerance, it is suggested that the term endotoxin immunity be substituted for endotoxin tolerance.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D002845:Chromatography; D002850:Chromatography, Gel; D004731:Endotoxins; D007108:Immune Tolerance; D007109:Immunity; D007114:Immunization; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M; D007136:Immunoglobulins; D012157:Mononuclear Phagocyte System; D010600:Pharmacology; D011817:Rabbits; D012106:Research; D014116:Toxicology; D014611:Vaccination; D005719:gamma-Globulins
D000906:Antibodies; D004731:Endotoxins; D007075:Immunoglobulin M; D007136:Immunoglobulins; D005719:gamma-Globulins
ANTIBODY FORMATION; CHROMATOGRAPHY; ENDOTOXINS; EXPERIMENTAL LAB STUDY; GAMMA GLOBULIN, 19S; IMMUNE TOLERANCE; IMMUNIZATION; IMMUNOELECTROPHORESIS; PHARMACOLOGY; RABBITS; RETICULOENDOTHELIAL SYSTEM; TOXICOLOGIC REPORT