NUCLEAR MEMBRANES OF CULTURED MAMMALIAN CELLS IN THE PERIOD PRECEDING DNA SYNTHESIS.
When kidney cells are cultured directly from the rabbit, the nuclear membranes undergo a change that can be measured as an increase in electrophoretic mobility. The change appears to begin immediately upon culture and is maximal 2 hours later, after which the mobility remains constant at the elevated level. Actinomycin D and p-fluorophenylalanine, but not EDTA or ionizing radiation, suppress the increase in nuclear electrophoretic mobility. With synchronously growing L cells, no change was detected in nuclei from cells taken during various parts of the division cycle.
D000818:Animals; D002467:Cell Nucleus; D004247:DNA; D003609:Dactinomycin; D004492:Edetic Acid; D004586:Electrophoresis; D007668:Kidney; D007739:L Cells; D008660:Metabolism; D051379:Mice; D009685:Nuclear Envelope; D010600:Pharmacology; D010649:Phenylalanine; D011817:Rabbits; D011830:Radiation Effects; D012106:Research
D003609:Dactinomycin; D010649:Phenylalanine; D004247:DNA; D004492:Edetic Acid
ACTINOMYCIN; CELL NUCLEUS; DNA; EDTA; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; KIDNEY; L CELLS; METABOLISM; PHARMACOLOGY; PHENYLALANINE; RABBITS; RADIATION EFFECTS