OXIDATION OF SECONDARY ALCOHOLS BY EXTRACTS OF A CORYNEBACTERIUM.
Rosenberg, Eugene (University of California, Los Angeles), and Paul Holmes. Oxidation of secondary alcohols by extracts of a Corynebacterium. J. Bacteriol. 89:1212-1216. 1965.-A Corynebacterium was isolated from soil by use of the enrichment culture technique. This Corynebacterium oxidized and grew on the chemically synthesized secondary alcohol, 3-tetrahydrofuranol. Extracts of this organism contained at least two different nicotinamide adenine dinucleotide-requiring soluble secondary alcohol dehydrogenases. These enzymes were distinguished by the relative rates at which they oxidized thymidylic acid and 3-tetrahydrofuranol. In addition to their substrate specificity, the two enzymes differed in pH optima and thermal stability. Also, the 3-tetrahydrofuranol dehydrogenase was induced by 3-tetrahydrofuranol, whereas the thymidylic acid dehydrogenase was constitutive.
D000429:Alcohol Oxidoreductases; D000438:Alcohols; D003352:Corynebacterium; D005663:Furans; D006863:Hydrogen-Ion Concentration; D009243:NAD; D009711:Nucleotides; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D012106:Research; D013379:Substrate Specificity; D013696:Temperature
D000438:Alcohols; D005663:Furans; D009711:Nucleotides; D009243:NAD; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP)
ALCOHOL OXIDOREDUCTASES; ALCOHOLS; CORYNEBACTERIUM; EXPERIMENTAL LAB STUDY; FURANS; HYDROGEN-ION CONCENTRATION; NAD; NUCLEOTIDES; OXIDATION-REDUCTION; TEMPERATURE