THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION.
A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.
D000818:Animals; D000906:Antibodies; D000941:Antigens; D002353:Carrier State; D002648:Child; D002845:Chromatography; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D007136:Immunoglobulins; D009393:Nephritis; D011135:Polysaccharides, Bacterial; D011817:Rabbits; D012106:Research; D012213:Rheumatic Fever; D018805:Sepsis; D013290:Streptococcal Infections; D013291:Streptococcus; D013297:Streptococcus pyogenes
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D007136:Immunoglobulins; D011135:Polysaccharides, Bacterial; C036112:streptococcal polysaccharide group A
ANTIBODIES; CARRIER STATE; CHILD; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; HEMAGGLUTINATION; HEMAGGLUTINATION INHIBITION TESTS; NEPHRITIS; POLYSACCHARIDES, BACTERIAL; RABBITS; RHEUMATIC FEVER; SEPTICEMIA; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS