CHARACTERIZATION OF CONJUGATION FACTORS IN ESCHERICHIA COLI CELL WALLS. I. INHIBITION OF RECOMBINATION BY CELL WALLS AND CELL EXTRACTS.
Lancaster, John H. (University of Texas, Austin), E. P. Goldschmidt, and Orville Wyss. Characterization of conjugation factors in Escherichia coli cell walls. I. Inhibition of recombination by cell walls and cell extracts. J. Bacteriol. 89:1478-1481. 1965.-An assay procedure was devised to determine quantitatively the interference with conjugation of cell-wall fragments from both male and female strains of Escherichia coli. The fertility of the donor is reflected in the assay. Phenol extracts from the cell walls were active, and chemical analysis suggested that the activity resided in the lipopolysaccharide fraction.
D002457:Cell Extracts; D002473:Cell Wall; D003470:Culture Media; D003593:Cytoplasm; D004926:Escherichia coli; D005823:Genetics; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011995:Recombination, Genetic; D012106:Research
D002457:Cell Extracts; D003470:Culture Media; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial
CULTURE MEDIA; CYTOPLASM; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; GENETICS; LIPOPOLYSACCHARIDES; POLYSACCHARIDES, BACTERIAL