A COMPARATIVE STUDY OF THE TRIPHENYLTETRAZOLIUM CHLORIDE (UROSCREEN) TEST AND CONVENTIONAL METHODS FOR THE DETECTION OF BACTERIURIA.
Acute urinary tract infection may be preceded by and active pyelonephritis may be associated with asymptomatic bacteriuria. Treatment of asymptomatic bacteriuria may prevent or arrest active, chronic pyelonephritis and its sequelae. Consequently, there is a need for a reliable and simple screening procedure to detect asymptomatic bacteriuria in large segments of the population.The reliability and practicability of tests advocated for the detection of bacteriuria, including the new chemical triphenyltetrazolium chloride (T.T.C.) (Uroscreen) test, were evaluated. Reliability was assessed by correlating results of these tests with bacterial counts of tested urines. Significant bacteriuria is defined as the presence of 100,000 or more organisms per ml. of urine.The T.T.C. (Uroscreen) test was positive in 92.5% of cases of bacteriuria; there were 7.5% false-negative and 2.8% false-positive results. Bacteria on Gram-stained smear were found in 95.5% of the cases of bacteriuria and in 14.6% of those with non-infected urine; pyuria (more than three leukocytes per high-power field), in 60% of those with bacteriuria and in 15.9% of those with presumably non-infected urine. Bacteria were conspicuous in the urinary sediment in 91.1% of cases of bacteriuria and in 3.7% of presumably non-infected urines.The T.T.C. (Uroscreen) test fulfilled the criteria for a reliable and simple screening procedure. It should be used concomitantly with other screening tests when the urine is examined routinely.
D001419:Bacteria; D001431:Bacteriological Techniques; D001437:Bacteriuria; D006801:Humans; D008403:Mass Screening; D011704:Pyelonephritis; D011776:Pyuria; D015203:Reproducibility of Results; D013194:Staining and Labeling; D013778:Tetrazolium Salts; D016482:Urinalysis; D014552:Urinary Tract Infections; D014556:Urine
D013778:Tetrazolium Salts; C009591:triphenyltetrazolium
BACTERIA; BACTERIOLOGICAL TECHNICS; BACTERIURIA; MASS SCREENING; TETRAZOLIUM SALTS; URINARY TRACT INFECTIONS; URINE