ISOLATION AND CHARACTERIZATION OF A TOXIC MOIETY OF LOW MOLECULAR WEIGHT FROM CLOSTRIDIUM BOTULINUM TYPE A.
Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, and Hardial S. Bains. Isolation and characterization of a toxic moiety of low molecular weight from Clostridium botulinum type A. J. Bacteriol. 89:1383-1386. 1965.-A toxic moiety of low molecular weight has been isolated from a type A strain of Clostridium botulinum, by a method involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. By means of electrophoresis and ultracentrifugation, the toxic substance was shown to be homogeneous; a molecular weight of 12,200 was calculated.
D064751:Ammonium Compounds; D000818:Animals; D000992:Antitoxins; D001681:Biological Assay; D002170:Canada; D002482:Cellulose; D011232:Chemical Precipitation; D002845:Chromatography; D003014:Clostridium botulinum; D047013:Clostridium botulinum type A; D003636:DEAE-Cellulose; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008970:Molecular Weight; D000644:Quaternary Ammonium Compounds; D012106:Research; D013431:Sulfates; D014118:Toxins, Biological; D014461:Ultracentrifugation
D064751:Ammonium Compounds; D000992:Antitoxins; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D014118:Toxins, Biological; D002482:Cellulose; D003636:DEAE-Cellulose
AMMONIUM COMPOUNDS; BIOLOGICAL ASSAY; CHROMATOGRAPHY; CLOSTRIDIUM BOTULINUM; DEAE-CELLULOSE; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; MICE; MOLECULAR WEIGHT; PRECIPITATION; SULFATES; TOXINS AND ANTITOXINS; ULTRACENTRIFUGATION