PRODUCTION OF 2-KETOGLUCONIC ACID BY SERRATIA MARCESCENS.
Production of 2-ketogluconic acid from glucose by fermentation with Serratia marcescens NRRL B-486 was studied in 20-liter stainless-steel fermentors. Conditions for 2-ketogluconic acid production included the following: glucose-salt medium, aeration rate of 0.75 volumes per volume per minute, agitation rate of 400 rev/min, temperature of 30 C, CaCO(3) to neutralize the acid formed, and a 5% (v/v) inoculum. Foaming was controlled with an antifoam agent added at intervals during the fermentation. When 120 g per liter of glucose were supplied, 95 to 100% yields of 2-ketogluconic acid were obtained in 16 hr. Larger amounts of glucose could be used in the fermentation provided that the carbohydrate was fed continuously. Continuous feeding of glucose to a total amount of 180 g per liter gave 95 to 100% yields of 2-ketogluconic acid in 24 hr; feeding glucose to a total amount of 240 g per liter gave 85 to 90% yields in 32 to 40 hr.
D050260:Carbohydrate Metabolism; D002845:Chromatography; D003470:Culture Media; D005285:Fermentation; D005942:Gluconates; D005947:Glucose / Q000378:metabolism*; D008660:Metabolism; D010600:Pharmacology; D012106:Research; D012706:Serratia marcescens; D013696:Temperature
D003470:Culture Media; D005942:Gluconates; C008229:2-ketogluconate; D005947:Glucose
CHROMATOGRAPHY; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; FERMENTATION; GLUCONATES; GLUCOSE METABOLISM; METABOLISM; PHARMACOLOGY; SERRATIA MARCESCENS; TEMPERATURE