CONJUGATIONS WITH GLUTATHIONE. THE ENZYMIC CONJUGATION OF SOME CHLOROCYCLOHEXENES.
1. alpha-3,4,5,6-Tetrachlorocyclohex-1-ene and gamma-2,3,4,5,6-pentachlorocyclohex-1-ene are conjugated with glutathione in vitro by a rat-liver enzyme that is probably glutathione S-aryltransferase. 2. Chlorocyclohexane and the alpha-, beta-, gamma- and delta-isomers of hexachlorocyclohexane were not substrates for rat-liver glutathione S-aryltransferase. 3. Glutathione-S-aryltransferase activity was present in tissue preparations of houseflies of insecticide-resistant and -susceptible strains. More activity was found in a dieldrin-resistant strain of houseflies fed on dieldrin than in either a dieldrin-resistant strain not fed on dieldrin or a control strain of dieldrin-susceptible houseflies. 4. Housefly soluble supernatant preparations converted S-(2-chloro-4-nitrophenyl)glutathione into the corresponding cysteine and mercapturic acid derivatives.
D000818:Animals; D002845:Chromatography; D003516:Cycloparaffins; D004026:Dieldrin; D005978:Glutathione / Q000031:analogs & derivatives; D005982:Glutathione Transferase; D001556:Hexachlorocyclohexane; D006793:Houseflies; D008099:Liver / Q000201:enzymology*; D008660:Metabolism; D051381:Rats; D012106:Research; D014166:Transferases
D003516:Cycloparaffins; C020610:S-(2-chloro-4-nitrophenyl)glutathione; D001556:Hexachlorocyclohexane; D014166:Transferases; D005982:Glutathione Transferase; D005978:Glutathione; D004026:Dieldrin
BENZENE HEXACHLORIDE; CHROMATOGRAPHY; CYCLOPARAFFINS; DIELDRIN; EXPERIMENTAL LAB STUDY; GLUTATHIONE; HOUSEFLIES; LIVER ENZYMOLOGY; METABOLISM; RATS; TRANSFERASES