THE ESTIMATION OF THE OXIDIZED AND REDUCED FORMS OF THE NICOTINAMIDE NUCLEOTIDES.
1. A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0.02mug. of NAD(+) or NADH or 0.01mug. of NADP(+) or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature.
D000273:Adipose Tissue; D000429:Alcohol Oxidoreductases; D000818:Animals; D006528:Carcinoma, Hepatocellular; D005954:Glucosephosphate Dehydrogenase; D008099:Liver; D008113:Liver Neoplasms; D008321:Mammary Glands, Animal; D009243:NAD; D009249:NADP; D009536:Niacinamide; D009711:Nucleotides; D010084:Oxidation-Reduction; D010600:Pharmacology; D010619:Phenazines; D010945:Plants, Edible; D011048:Polarography; D051381:Rats; D012106:Research
D009711:Nucleotides; D010619:Phenazines; D009243:NAD; D009536:Niacinamide; D009249:NADP; D000429:Alcohol Oxidoreductases; D005954:Glucosephosphate Dehydrogenase
ADIPOSE TISSUE; ALCOHOL OXIDOREDUCTASES; EXPERIMENTAL LAB STUDY; GLUCOSEPHOSPHATE DEHYDROGENASE; HEPATOMA; LIVER; MAMMAE; NAD; NADP; PHARMACOLOGY; PHENAZINES; PLANTS, EDIBLE; POLAROGRAPHY; RATS