ISOLATION AND CHARACTERIZATION OF A NEW ANTIBIOTIC, ABBOTT 29119, FROM STREPTOMYCES ERYTHREUS.
A new antibiotic was isolated from fermentations of Streptomyces erythreus designed for erythromycin A production. Isolation of this compound was accomplished by use of ion-exchange chromatography and countercurrent distribution. It crystallized from methyl isobutyl ketone in colorless acicular plates which melted at 127 to 130 C. The empirical formula was C(42)H(75)NO(14) with a molecular weight of 820. Electrometric titration in 66% dimethylformamide showed an apparent pKa of 8.4. Nuclear magnetic resonance, infrared, and ultraviolet absorption indicated a close resemblance of the compound to known erythromycins, although its chromatographic behavior and solubility differed from those of the known erythromycins. Its antimicrobial spectrum was similar to erythromycin A, but the minimal inhibitory concentrations were much higher.
D000900:Anti-Bacterial Agents; D000904:Antibiotics, Antitubercular; D001412:Bacillus subtilis; D001419:Bacteria; D001433:Bacteriolysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004351:Drug Resistance; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis; D004917:Erythromycin; D004926:Escherichia coli; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D011511:Proteus; D012106:Research; D016930:Saccharopolyspora; D012486:Salmonella typhimurium; D013210:Staphylococcus; D013302:Streptomyces
D000900:Anti-Bacterial Agents; D000904:Antibiotics, Antitubercular; D004917:Erythromycin
ANTIBIOTICS; BACILLUS SUBTILIS; BACTERIOLYSIS; CHEMISTRY, PHYSICAL; CHROMATOGRAPHY; DRUG RESISTANCE, MICROBIAL; ERYTHROMYCIN; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; FERMENTATION; PROTEUS; SALMONELLA TYPHIMURIUM; STAPHYLOCOCCUS; STREPTOCOCCUS FAECALIS; STREPTOMYCES