PRODUCTION OF AFLATOXINS IN SUBMERGED CULTURE.
Aflatoxins can be produced on a synthetic medium in submerged culture. Glucose, sucrose, or fructose are the preferred carbon sources, and Casamino Acids are the preferred nitrogen source. Ammonia is almost as good a nitrogen source. Zinc is required at levels of at least 0.4 mg per liter. Concentrations of aflatoxin of 60 to 80 mg per liter (as determined by optical-density measurements of a chloroform extract of the unfiltered broth) can readily be obtained in indented shake flasks; somewhat lower yields were obtained in 5-liter fermentors.
D000348:Aflatoxins; D000596:Amino Acids / Q000378:metabolism*; D000641:Ammonia; D064751:Ammonium Compounds; D000992:Antitoxins; D001230:Aspergillus; D050260:Carbohydrate Metabolism; D002244:Carbon; D002273:Carcinogens; D003470:Culture Media; D005632:Fructose; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D009566:Nitrates; D009584:Nitrogen; D010600:Pharmacology; D000644:Quaternary Ammonium Compounds; D012106:Research; D013395:Sucrose; D014118:Toxins, Biological; D014131:Trace Elements
D000348:Aflatoxins; D000596:Amino Acids; D064751:Ammonium Compounds; D000992:Antitoxins; D002273:Carcinogens; D003470:Culture Media; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D014118:Toxins, Biological; D014131:Trace Elements; C017721:casamino acids; D005632:Fructose; D013395:Sucrose; D002244:Carbon; D000641:Ammonia; D005947:Glucose; D009584:Nitrogen
AMINO ACID METABOLISM; AMMONIUM COMPOUNDS; ASPERGILLUS; CARBOHYDRATE METABOLISM; CARCINOGENS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; NITRATES; PHARMACOLOGY; TOXINS AND ANTITOXINS; TRACE ELEMENTS