COOLING DEVICE FOR USE WITH A SONIC OSCILLATOR.
A cooling cell is described that facilitates maintenance of biological materials at low temperatures during prolonged sonic treatment. Using this cell and a Branson S-75 Sonifier, I examined temperatures and the release of protein and enzyme activity from suspensions of Saccharomyces cerevisiae. With the Sonifier at full power, it was possible to maintain cell temperature within 9 C of the cooling-bath temperature, and to disrupt 10% (w/v) suspensions of S. cerevisiae in 10 min.
D001431:Bacteriological Techniques; D003080:Cold Temperature; D004864:Equipment and Supplies; D008283:Maintenance; D009991:Oscillometry; D011506:Proteins; D012106:Research; D012440:Saccharomyces; D012441:Saccharomyces cerevisiae; D013696:Temperature; D014116:Toxicology
D011506:Proteins
BACTERIOLOGICAL TECHNICS; COLD; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; OSCILLOMETRY; SACCHAROMYCES; TOXICOLOGIC REPORT