DETERMINATION OF BASE RATIOS OF SIX RIBONUCLEIC ACID BACTERIOPHAGES SPECIFIC TO ESCHERICHIA COLI.
1. A method is described for the isolation of single-stranded-RNA coliphages. Two of the six RNA coliphages investigated were new strains. 2. The base ratios of six RNA coliphages were determined by labelling the host bacterium with [(32)P]-phosphate, purification of the radioactive coliphages and separation of 2',3'-ribonucleotides liberated by alkaline hydrolysis of the coliphage RNA. 3. All six of the coliphages were morphologically similar, contained single-stranded RNA, and had sedimentation coefficient 80+/-5s. 4. The six RNA coliphages fell into two distinct groups, both serologically and in terms of their RNA base ratios.
D000225:Adenine; D001435:Bacteriophages; D001482:Base Composition; D001669:Biochemical Phenomena; D001671:Biochemistry; D002845:Chromatography; D003090:Coliphages; D003596:Cytosine; D004247:DNA; D003851:Deoxyribonucleases; D004586:Electrophoresis; D004926:Escherichia coli; D006147:Guanine; D006868:Hydrolysis; D007475:Ion Exchange Resins; D009711:Nucleotides; D012313:RNA; D012367:RNA, Viral; D012106:Research; D012260:Ribonucleases; D012270:Ribosomes; D014498:Uracil
D007475:Ion Exchange Resins; D009711:Nucleotides; D012367:RNA, Viral; D014498:Uracil; D006147:Guanine; D012313:RNA; D003596:Cytosine; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D000225:Adenine
ADENINE; BIOCHEMISTRY; CHROMATOGRAPHY; COLIPHAGES; CYTOSINE; DEOXYRIBONUCLEASE; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GUANINE; ION EXCHANGE RESINS; NUCLEOTIDES; RIBONUCLEASE; RIBOSOMES; RNA, VIRAL; URACIL