PHYSICOCHEMICAL AND BIOLOGICAL STUDIES ON VARIOUS PREPARATIONS OF TUBERCULIN PURIFIED PROTEIN DERIVATIVE.
Tuberculin purified protein derivative (PPD) has been prepared by seven different precipitation methods from culture filtrate of Mycobacterium tuberculosis var. hominis. It was found to contain 48 to 99% tuberculoprotein, depending on the method of precipitation. The remaining percentage is represented by nucleic acid, polysaccharide, and ash. Activation analysis on tuberculin PPD and on tubercle bacilli has revealed the presence of trace elements. The molecular weight of tuberculin PPD has been found to be of the order of 14,800 to 27,800. The biological activity of tuberculin PPD varies from lot to lot and from method to method. A correlation between its molecular weight and its biological activity seems to exist.
D064751:Ammonium Compounds; D000818:Animals; D001426:Bacterial Proteins; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004586:Electrophoresis; D006168:Guinea Pigs; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis; D009696:Nucleic Acids; D010600:Pharmacology; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D012106:Research; D013057:Spectrum Analysis; D013431:Sulfates; D014131:Trace Elements; D014238:Trichloroacetic Acid; D014373:Tuberculin; D014461:Ultracentrifugation
D064751:Ammonium Compounds; D001426:Bacterial Proteins; D009696:Nucleic Acids; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D014131:Trace Elements; D014373:Tuberculin; D014238:Trichloroacetic Acid
AMMONIUM COMPOUNDS; BACTERIAL PROTEINS; CHEMISTRY; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; GUINEA PIGS; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACIDS; PHARMACOLOGY; POLYSACCHARIDES; PRECIPITATION; SPECTRUM ANALYSIS; SULFATES; TRACE ELEMENTS; TRICHLOROACETIC ACID; TUBERCULIN; ULTRACENTRIFUGATION