POLYRIBOSOMES FROM ESCHERICHIA COLI: ENZYMATIC METHOD FOR ISOLATION.
Polyribosomes can be rapidly extracted from Escherichia coli by sequential passage of the cells through solutions of a chelating agent and lysozyme in a centrifugal field. Biosynthesis of protein in whole cells and in a cell-free system occurs almost exclusively in the polyribosomes.
D000818:Animals; D001669:Biochemical Phenomena; D001671:Biochemistry; D002474:Cell-Free System; D004492:Edetic Acid; D004721:Endoplasmic Reticulum; D004926:Escherichia coli; D009113:Muramidase; D011132:Polyribosomes; D012270:Ribosomes
D004492:Edetic Acid; D009113:Muramidase
BIOCHEMISTRY; EDTA; ENDOPLASMIC RETICULUM; ESCHERICHIA COLI; MURAMIDASE; RIBOSOMES