ENZYMATIC DEACYLATION OF S35-BENZYLPENICILLIN.
Pruess, David L. (University of Wisconsin, Madison), and Marvin J. Johnson. Enzymatic deacylation of S(35)-benzylpenicillin. J. Bacteriol. 90:380-383. 1965.-S(35)-benzylpenicillin, penicilloic acid, and penilloic acid were deacylated by cell suspensions of Escherichia coli and Micrococcus roseus. Both cultures deacylated penicillin most rapidly and penilloic acid least rapidly. The deacylase activity of M. roseus against penicilloic acid was cell-bound, probably requiring a metal ion for activity.
D000096:Acetone; D000215:Acylation; D002021:Buffers; D002498:Centrifugation; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D003470:Culture Media; D004926:Escherichia coli; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008660:Metabolism; D008837:Micrococcus; D010397:Penicillanic Acid / Q000031:analogs & derivatives; D010400:Penicillin G / Q000031:analogs & derivatives; D010406:Penicillins; D010600:Pharmacology; D010648:Phenylacetates; D011874:Radiometry; D012106:Research; D013460:Sulfur Isotopes
D002021:Buffers; D003470:Culture Media; D010406:Penicillins; D010648:Phenylacetates; D013460:Sulfur Isotopes; C008782:penicilloic acid; D000096:Acetone; D010397:Penicillanic Acid; C017609:penilloic acid; D010400:Penicillin G
ACETONE; BUFFERS; CENTRIFUGATION; CHEMISTRY; CHROMATOGRAPHY; CULTURE MEDIA; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; FERMENTATION; HYDROGEN-ION CONCENTRATION; METABOLISM; MICROCOCCUS; PENICILLIN G; PHARMACOLOGY; PHENYLACETATES; RADIOMETRY; SULFUR ISOTOPES