IMMUNITY IN EXPERIMENTAL SYPHILIS. 3. ATTENUATION OF VIRULENT TREPONEMA PALLIDIUM BY GAMMA-IRRADIATION.
Miller, James N. (University of California School of Medicine, Los Angeles). Immunity in experimental syphilis. III. Attenuation of virulent Treponema pallidum by gamma-irradiation. J. Bacteriol. 90:297-301. 1965.-Virulent, freshly isolated cells of Treponema pallidum strain Nichols suspended in a 50% rabbit serum-saline solution and exposed to a gamma-irradiation dosage of 652,800 r were rendered noninfectious without apparent loss of motility or change in observable morphological and staining characteristics. Although 5 x 10(7) gamma-irradiated organisms failed to elicit an immobilizing antibody response in rabbits, the same organisms retained their capacity to react with classical T. pallidum-immobilizing antibody.
D000818:Animals; D000937:Antigen-Antibody Reactions; D004396:Coloring Agents; D007109:Immunity; D011817:Rabbits; D011830:Radiation Effects; D012106:Research; D013194:Staining and Labeling; D013587:Syphilis; D014208:Treponema; D014209:Treponema Immobilization Test; D014210:Treponema pallidum; D014774:Virulence
D004396:Coloring Agents
ANTIGEN-ANTIBODY REACTIONS; EXPERIMENTAL LAB STUDY; IMMUNITY; RABBITS; RADIATION EFFECTS; STAINS AND STAINING; SYPHILIS; TREPONEMA IMMOBILIZATION TEST; TREPONEMA PALLIDUM; VIRULENCE