THE EXTRACTION OF PLASMA 3-HYDROXY-17-OXO STEROID SULPHATES AND THE MEASUREMENT OF THE CONSTITUENT DEHYDROEPIANDROSTERONE SULPHATE AND ANDROSTERONE SULPHATE.
1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and androsterone sulphate concentrations of normal and pathological plasmas are given.
D015068:17-Ketosteroids; D000738:Androsterone / Q000031:analogs & derivatives; D001774:Blood Chemical Analysis; D001793:Blood Preservation; D002845:Chromatography; D003124:Colorimetry; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D005043:Etiocholanolone; D010505:Familial Mediterranean Fever; D005965:Glucuronates; D006801:Humans; D010710:Phosphates; D013256:Steroids; D013431:Sulfates
D015068:17-Ketosteroids; D005965:Glucuronates; D010710:Phosphates; D013256:Steroids; D013431:Sulfates; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D005043:Etiocholanolone; D000738:Androsterone; C026905:androsterone sulfate
17-KETOSTEROIDS; ANDROSTERONE; BLOOD CHEMICAL ANALYSIS; BLOOD PRESERVATION; CHROMATOGRAPHY; COLORIMETRY; DEHYDROEPIANDROSTERONE; GLUCURONATES; PERIODIC DISEASE; PHOSPHATES; SULFATES