INTERFERON-LIKE ACTIVITY OF EXTRACTS OF HUMAN SPLEEN.
Extracts of human splenic tissue were found to resemble interferon in (a) their physicochemical properties, and (b) their mode of action when tested in vivo in the rabbit, using vaccinia as the challenge virus. The methods used to obtain (1) the crude extract and (2) a partially purified final fraction are described. The latter was found to be non-dialysable, stable over a pH range of 2-11, and relatively heat resistant. On analysis it appeared to be, or was associated with, a glycoprotein.
D000818:Animals; D000998:Antiviral Agents; D001688:Biological Products; D002241:Carbohydrates; D055598:Chemical Phenomena; D002621:Chemistry; D002623:Chemistry Techniques, Analytical; D006601:Hexoses; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons; D007793:Lagomorpha; D011506:Proteins; D011817:Rabbits; D012106:Research; D013154:Spleen; D014020:Tissue Extracts; D014616:Vaccinia virus
D000998:Antiviral Agents; D001688:Biological Products; D002241:Carbohydrates; D006601:Hexoses; D011506:Proteins; D014020:Tissue Extracts; D007372:Interferons
ANTIVIRAL AGENTS; BIOLOGICAL PRODUCTS; CARBOHYDRATES; CHEMISTRY; CHEMISTRY, ANALYTICAL; EXPERIMENTAL LAB STUDY; HEAT; HYDROGEN-ION CONCENTRATION; INTERFERON; PROTEINS; RABBITS; SPLEEN; TISSUE EXTRACTS; VACCINIA VIRUS