NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD.
Direct smears from female patients have been considered unreliable for the detection of Neisseria gonorrhoeae by fluorescent-antibody (FA) methods because of inadequate background contrast of the fluorescent-stained smears and a scarcity of organisms on the smear. Evans blue dye employed as a counterstain eliminated the nonspecific background staining and increased the reliability of the direct FA procedure. Direct smears demonstrating positive fluorescence were obtained from 86% of a group of culturally positive named female contacts. The FA-counterstain technique is as sensitive as the presently recommended cultural procedures.
D004396:Coloring Agents; D003470:Culture Media; D003933:Diagnosis; D005260:Female; D005455:Fluorescent Antibody Technique; D019085:Fluorescent Antibody Technique, Direct; D006069:Gonorrhea; D006801:Humans; D009344:Neisseria gonorrhoeae; D015203:Reproducibility of Results; D012106:Research; D012749:Sexually Transmitted Diseases; D013194:Staining and Labeling; D014626:Vaginal Smears
D004396:Coloring Agents; D003470:Culture Media
CULTURE MEDIA; DIAGNOSIS; EXPERIMENTAL LAB STUDY; FLUORESCENT ANTIBODY TECHNIC; NEISSERIA GONORRHOEAE; STAINS AND STAINING; VAGINAL SMEARS; VENEREAL DISEASES