LYSOSOMAL FRACTIONS FROM TRANSITIONAL EPITHELIUM.
Histochemical data suggested that the so called lipoid granules of transitional epithelium in some species are equivalent to lysosomes. Scrapings of bovine and canine transitional epithelium were subjected to differential centrifugation to confirm this identification biochemically. Fractions of rat liver, the classic source of lysosomes, were also prepared by the same methods to compare with the fractions obtained from urinary epithelium. In contrast to rat liver, uroepithelial fractions with a high relative specific activity for hydrolases were sedimented before the heavy mitochondria. Microscopically, these fractions contained the highest proportion of lipoid granules. The size and sedimentation characteristics of lysosomes from transitional epithelium more closely resembled those of lysosomes derived from rat kidney than those isolated from liver.
D000135:Acid Phosphatase; D000818:Animals; D002417:Cattle; D002498:Centrifugation; D055598:Chemical Phenomena; D002621:Chemistry; D003594:Cytoplasmic Granules; D004285:Dogs; D003576:Electron Transport Complex IV; D004848:Epithelium; D005966:Glucuronidase; D008055:Lipids; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes; D008928:Mitochondria; D011048:Polarography; D051381:Rats; D012106:Research; D001743:Urinary Bladder
D008055:Lipids; D003576:Electron Transport Complex IV; D000135:Acid Phosphatase; D005966:Glucuronidase
ACID PHOSPHATASE; BLADDER; CATTLE; CENTRIFUGATION; CHEMISTRY; CYTOCHROME OXIDASE; DOGS; EPITHELIUM; EXPERIMENTAL LAB STUDY; GLUCURONIDASE; LIPIDS; LIVER ENZYMOLOGY; LYSOSOMES; MITOCHONDRIA; POLAROGRAPHY; RATS