RESISTANCE OF CLOSTRIDIUM PERFRINGENS TYPE A SPORES TO GAMMA-RADIATION.
The radiation resistance of the spores of a classical strain and of an atypical, heat-resistant strain of Clostridium perfringens was determined. Spores were produced in Ellner's and in a Trypticase broth medium. Approximately 10(6) viable spores per milliliter were suspended in 0.06 m phosphate buffer and irradiated with gamma rays from cobalt-60; the survivors were counted in Tryptone-yeast extract-agar by the Prickett-tube technique. Radiation D values for spores of the atypical strain in phosphate buffer and in cooked-meat broth were 0.23 and 0.30 Mrad, respectively, and the D value of the classical strain was 0.25 Mrad in phosphate buffer. Spores of the classical and atypical strains of C. perfringens type A are characterized by differences in heat resistance; yet, all strains tested demonstrated similar radiation resistance. Also, the spores were more resistant to ionizing radiation in cooked-meat broth than in phosphate buffer.
D000362:Agar; D050260:Carbohydrate Metabolism; D003016:Clostridium perfringens; D003037:Cobalt Radioisotopes; D003470:Culture Media; D005285:Fermentation; D005720:Gamma Rays; D005780:Gelatin; D006358:Hot Temperature; D007211:Indoles; D009566:Nitrates; D010740:Phospholipases; D011830:Radiation Effects; D012106:Research; D013170:Spores; D013171:Spores, Bacterial; D013440:Sulfides
D003037:Cobalt Radioisotopes; D003470:Culture Media; D007211:Indoles; D009566:Nitrates; D013440:Sulfides; D005780:Gelatin; D000362:Agar; D010740:Phospholipases
CARBOHYDRATE METABOLISM; CLOSTRIDIUM PERFRINGENS; CULTURE MEDIA; EXPERIMENTAL LAB STUDY; FERMENTATION; GELATIN; INDOLES; NITRATES; PHOSPHOLIPASE; RADIATION EFFECTS; SPORES; SULFIDES