LOCALIZATION OF THE SUCCINIC DEHYDROGENASE SYSTEM IN ESCHERICHIA COLI USING COMBINED TECHNIQUES OF CYTOCHEMISTRY AND ELECTRON MICROSCOPY.
The activity of the succinic dehydrogenase system was studied in Escherichia coli utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by the deposition of aggregates of TNBT-formazan, was found associated with the site of the plasma membrane of the bacterium.
D002462:Cell Membrane; D055598:Chemical Phenomena; D002621:Chemistry; D003470:Culture Media; D004583:Electrons; D004926:Escherichia coli; D006651:Histocytochemistry; D008853:Microscopy; D008854:Microscopy, Electron; D012106:Research; D013385:Succinate Dehydrogenase; D013778:Tetrazolium Salts
D003470:Culture Media; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; D013385:Succinate Dehydrogenase
CHEMISTRY; CULTURE MEDIA; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; MICROSCOPY, ELECTRON; SUCCINATE DEHYDROGENASE; TETRAZOLIUM SALTS