Purification of staphylococcal alpha-hemolysin.
Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914-918. 1962. - Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.
D000992:Antitoxins / Q000737:chemistry*; D001427:Bacterial Toxins; D004566:Electrodes; D005098:Exotoxins; D006460:Hemolysin Proteins; D006761:Hospitals; D008970:Molecular Weight; D013210:Staphylococcus; D014118:Toxins, Biological / Q000737:chemistry*
D000992:Antitoxins; D001427:Bacterial Toxins; D005098:Exotoxins; D006460:Hemolysin Proteins; D014118:Toxins, Biological; C021091:staphylococcal alpha-toxin
STAPHYLOCOCCUS; TOXINS AND ANTITOXINS/chemistry