Enzymatic utilization of glucose by a basidiomycete.
Meloche, H. P., Jr. (Northern Regional Research Laboratory, Peoria, Ill.). Enzymatic utilization of glucose by a basidiomycete. J. Bacteriol. 83:766-774. 1962.-Cell-free extracts of acetone-dried Lactarius torminosus NRRL 2900 were prepared. These extracts contained hexokinase. They also contained triphosphopyridine nucleotide (TPN)-specific glucose-6-phosphate dehydrogenase and catalyzed the reduction of TPN in the presence of d-fructose-6-phosphate, 6-phospho-d-gluconic acid (6PG), and d-ribose-5-phosphate (R5P). Aged preparations oxidized d-glucose-6-phosphate (G6P) to 6PG, whereas fresh preparations oxidized G6P to a pentose with the uptake of 1 mumole of O(2) and the evolution of 1 mumole of CO(2) per mumole of G6P. Evidence for the action of transketolase in the metabolism of R5P by cell-free extracts was obtained.Cell-free preparations lacked hexosediphosphate enzymes. Triosephosphate isomerase and F6P kinase could not be demonstrated; however, aldolase activity was present. Evidence is presented for the conversion of d-glyceraldehyde-3-phosphate to pyruvate. In addition, phosphohexoisomerase was demonstrated. It appears that a hexosemonophosphate pathway operates in L. torminosus extracts and may be the major mechanism of glucose dissimilation in this organism.
D001487:Basidiomycota; D050260:Carbohydrate Metabolism; D004798:Enzymes / Q000378:metabolism*; D005636:Fructosephosphates; D005658:Fungi / Q000378:metabolism*; D005942:Gluconates; D005947:Glucose / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase; D010429:Pentoses; D011773:Pyruvates; D012267:Ribosemonophosphates; D014174:Transketolase
D004798:Enzymes; D005636:Fructosephosphates; D005942:Gluconates; D010429:Pentoses; D011773:Pyruvates; D012267:Ribosemonophosphates; C031626:ribose-5-phosphate; C027618:fructose-6-phosphate; D014174:Transketolase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; C008884:6-phosphogluconic acid
ENZYMES/metabolism; FUNGI/metabolism; GLUCOSE/metabolism