L-Alanine dehydrogenase: a mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores.
O'Connor, R. J. (University of Wisconsin, Madison), and Harlyn O. Halvorson. L-Alanine dehydrogenase: A mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores. J. Bacteriol. 82:706-713. 1961.-A study has been undertaken of the properties and specificity of germination of spores of Bacillus cereus strain T. In the absence of additional carbon sources, only l-alanine, l-alpha-NH(2)-n-butyric acid, and l-cysteine were effective germinating agents. The physical properties of germination, induced by l-alanine and l-alpha-NH(2)-n-butyric acid following extended heat shock, were in close agreement with those of l-alanine dehydrogenase. The specificity of the germination system, as well as amino acid deamination in vivo, support the view that l-alanine dehydrogenase activity is essential for germination and that the enzyme serves as the initial binding site for l-alanine in heat-shocked spores.
D000409:Alanine; D050788:Alanine Dehydrogenase; D000596:Amino Acids / Q000494:pharmacology*; D001407:Bacillus / Q000502:physiology*; D001409:Bacillus cereus; D003545:Cysteine; D010088:Oxidoreductases / Q000494:pharmacology*; D013171:Spores, Bacterial
D000596:Amino Acids; D010088:Oxidoreductases; D050788:Alanine Dehydrogenase; D003545:Cysteine; D000409:Alanine
AMINO ACIDS/pharmacology; BACILLUS/physiology; DEHYDROGENASES/pharmacology