Hydrolysis of adenosine triphosphate by crystalline yeast pyrophosphatase. Effect of zinc and magnesium ions.
Schlesinger and Coon's report that crystalline yeast inorganic pyrophosphatase, in addition to its known ability to hydrolyze inorganic pyrophosphate in the presence of Mg ions, is also able to catalyze the hydrolysis of ATP and ADP in the presence of Zn ions was confirmed. A systematic study showed that the ratio of 370 of PPase-Mg over ATPase-Zn activities per milligram protein in various preparations of pyrophosphatase obtained in the course of isolation of crystalline pyrophosphatase from baker's yeast was nearly identical in all the preparations, independent of their purity. The course of hydrolysis of ATP by crystalline pyrophosphatase in the presence of Zn was carried out with the aid of ion exchange on Dowex 1. The finding of Schlesinger and Coon that the hydrolysis proceeds from ATP to ADP and then slowly to AMP was confirmed. The kinetics of the first phase of the reaction was found to depend on the molar ratio of Zn/ATP in the reaction mixture. Mg ions in the presence of Zn ions have an accelerating effect on the rate of hydrolysis of ATP. This suggests strongly that both activities-ATPase and PPase-are manifestations of the same active group in the protein molecule of crystalline pyrophosphatase.
D000227:Adenine Nucleotides / Q000737:chemistry*; D000255:Adenosine Triphosphate; D002384:Catalysis; D011756:Diphosphates; D006868:Hydrolysis; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D011755:Pyrophosphatases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae; D015032:Zinc / Q000737:chemistry*
D000227:Adenine Nucleotides; D011756:Diphosphates; C033875:zinc adenosine triphosphate; C107241:diphosphoric acid; D000255:Adenosine Triphosphate; D011755:Pyrophosphatases; D008274:Magnesium; D015032:Zinc
ADENOSINE PHOSPHATES/chemistry; MAGNESIUM/chemistry; PYROPHOSPHATASES/chemistry; ZINC/chemistry