Effect of 8-azaguanine on growth and viability of Bacillus megaterium.
Mangalo, R. (University of Illinois, Urbana) and J. T. Wachsman. Effect of 8-azaguanine on growth and viability of Bacillus megaterium. J. Bacteriol. 83:27-34. 1962.-The addition of 8-azaguanine to exponentially growing cells of Bacillus megaterium results in an inhibition of growth after a lag of approximately 30 min. However, 8-azaguanine-2-C(14) is incorporated into the nucleic acids in a linear fashion without a detectable lag. The inhibitory action is reversed by purines and their derivatives, but not by uridine, thymidine, or cytidine. 8-Azaguanine is bactericidal, especially under conditions where growth (ribonucleic acid synthesis) is possible. Growth in the presence of a complete amino acid mixture, either before or during exposure to 8-azaguanine, increases the rate of killing. Chloramphenicol has little or no effect on the bactericidal action of the analogue.
D000596:Amino Acids; D000963:Antimetabolites / Q000494:pharmacology*; D001375:Azaguanine; D001407:Bacillus / Q000494:pharmacology*; D001410:Bacillus megaterium; D009696:Nucleic Acids; D011687:Purines / Q000494:pharmacology*; D012313:RNA; D013936:Thymidine; D014529:Uridine
D000596:Amino Acids; D000963:Antimetabolites; D009696:Nucleic Acids; D011687:Purines; D012313:RNA; D001375:Azaguanine; D013936:Thymidine; C030985:purine; D014529:Uridine
ANTIMETABOLITES/pharmacology; BACILLUS/pharmacology; PURINES/pharmacology