Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide.
This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.
D000447:Aldehydes; D004583:Electrons; D006652:Histological Techniques; D008853:Microscopy; D008854:Microscopy, Electron; D009474:Neurons / Q000033:anatomy & histology*; D009992:Osmium; D009993:Osmium Tetroxide; D010477:Perfusion
D000447:Aldehydes; D009992:Osmium; D009993:Osmium Tetroxide
HISTOLOGICAL TECHNIQUES; MICROSCOPY, ELECTRON; NEURONS/anatomy and histology; OSMIUM