Comparison of virology and immunology in SHIV 89.6 proviral DNA and virus-inoculated rhesus macaques.
Inoculation of cats, goats and monkeys with plasmids encoding full-length proviral genomes results in persistent lentiviral infections. This system could be used as a method for administration of an attenuated human immunodeficiency virus (HIV) vaccine. Here, we compare the virology and immunology in rhesus macaques inoculated with either simian/human immunodeficiency virus 89.6 (SHIV 89.6) virus or a plasmid containing the SHIV 89.6 proviral genome. There was a delay in appearance of systemic infection in DNA-inoculated animals compared with virus-inoculated animals, but otherwise the pattern of infection was similar. The serum immunoglobulin G anti-simian immunodeficiency virus (SIV) binding antibody response in DNA-inoculated animals was also delayed compared with virus-inoculated animals, but ultimately there was no difference between live virus and DNA-inoculation in the ability to induce the anti-SIV immune responses that were measured. Thus, the data support the concept that plasmid DNA encoding an attenuated virus could be used instead live virus for vaccination.
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D016679:Genome, Viral; D006678:HIV / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008253:Macaca mulatta; D010957:Plasmids / Q000276:immunology; D011533:Proviruses / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014765:Viral Vaccines