[Inhibition of human laryngeal cancer Hep-2 cells by taxol].
OBJECTIVE
To assess the inhibition of taxol on human laryngeal cancer Hep-2 cell line in vitro.


METHODS
Human larynx cancer cell line. Hep 2, was used for this experiment, Hep-2 cells were exposed to taxol at different concentration for 6, 12, 24, 48, and 72 h, then the cell cycle analysis was performed with flow cytometry; growth curves of the cell line were drawn with MTT assay; DNA ladder was detected by agarose gel electrohoresis.


RESULTS
The Hep-2 cells developed a G2/M block and came into apoptosis after taxol exposured at different concentration for 6, 12, 24, 48, and 72 h. The percentage of the cell population in G2/M was higher at taxol longer exposure time and higher concentration. The Hep-2 cells growth for taxol was significantly inhibited, and the growth inhibition effect of taxol on Hep-2 cells was time and dosage dependent.


CONCLUSIONS
Taxol can block Hep-2 cells in G2/M, The induction of apoptosis Hep-2 cells by taxol was closely associated with mitotic arrest of cell cycle.
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel