[Differential effect of preservative solutions (UW vs HTK) on mitochondrial redux status and energy metabolism during liver ischemia with oxygen persufflation].
Venous-systemic oxygen persufflation (VSOP) was performed in rat livers stored at 4 degrees C in either UW or HTK preservation solution. Since tissue anoxia is associated with a transformation of cellular NAD+ to NADH and the latter fluoresces upon UV-epiillumination, homogeneity and intensity of liver oxygenation could be analysed by intravital microscopic detection of NADH fluorescence. VSOP resulted in a significant decrease of the NADH signal, documenting effective tissue oxygenation in both UW and HTK. This effect was quite homogeneous (spatial variance < 15%). After 48 h of cold storage tissue levels of ATP (mumol/g dry weight) were increased upon VSOP in UW to 17.3 +/- 4.8 but only to 2.9 +/- 0.6 in HTK, while ATP amounted to less than 0.4 without VSOP in either of the groups. It is concluded that VSOP is an appropriate tool to prevent alterations of the hepatic redox status during ischemic preservation in UW and HTK. Metabolic preservation of energy-rich adenine nucleotides seems to be largely improved in combination with UW compared with HTK.
D000241:Adenosine / Q000494:pharmacology*; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D007327:Insufflation; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000008:administration & dosage*; D010101:Oxygen Consumption / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology*; D011343:Procaine / Q000494:pharmacology*; D011887:Raffinose / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen