Molecular dissection of the vaccinia virus I7L core protein proteinase.
The vaccinia virus I7L gene product is predicted to be a cysteine proteinase and is demonstrated in this study to be responsible for cleavage of each of the three major core protein precursors (P4a, P4b, and P25K) in vivo. Mutagenesis of the putative catalytic triad of I7L or of the cleavage sites in the core protein precursors inhibits processing. A truncated protein lost the ability to cleave the core protein precursors.
D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000378:metabolism; D013329:Structure-Activity Relationship; D014616:Vaccinia virus / Q000201:enzymology*; D014758:Viral Core Proteins / Q000737:chemistry* / Q000502:physiology
D011498:Protein Precursors; D014758:Viral Core Proteins; D003546:Cysteine Endopeptidases