[Chimerism in allogeneic mini-transplantation].
The need of quantitation of chimerism is increasing as mini-transplantation has been on trial and they are possibly become the mixed chimeras. FISH method using XY chromosome as a marker is effective in heterosexual transplantation, And STR-PCR method is effective in homosexual transplantation. There is wide range in strength of pre-conditioning in Mini-transplantation; almost all stronger pre-conditioning with fludarabine end up in completely donor-type, and the pre-conditioning with ATG, TBL, L-PAM, M-Pred, 40% became mixed chimera but they all became completely donor type with DLI. There is a report that there are less GVHD with mixed chimera. To decide which pre-conditioning to use, we need to examine the rate of the relapse.
D000818:Animals; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017710:Lymphocyte Transfusion; D016176:T-Lymphocyte Subsets; D018183:Transplantation Chimera / Q000235:genetics; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous