Conjugate DNAzymes.
Conjugate DNAzymes were synthesized by solid phase fragment condensation and their biological properties were characterized. They have increased affinity to target RNA, enhanced stability against DNase 1 digestion and comparable or higher RNA cleaving activity compared with native and also phosphorothioate DNA zymes. It was also demonstrated that conjugate DNAzymes could inhibit BCR-ABL tyrosine kinase in cellular lysis of human leukemia cell line. Consequently DNAzymes can be expected to act effectively in cellular system and also in vivo system.
D001483:Base Sequence; D021881:DNA, Catalytic / Q000138:chemical synthesis / Q000737:chemistry*; D016044:Fusion Proteins, bcr-abl; D006801:Humans; D007938:Leukemia / Q000201:enzymology; D010455:Peptides / Q000737:chemistry; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured
D021881:DNA, Catalytic; D010455:Peptides; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl