Ethanol production by Saccharomyces cerevisiae grown in sugarcane blackstrap molasses through a fed-batch process: optimization by response surface methodology.
We studied the effect of reactor filling time (T) (3-5 h), initial mass of inoculum (M) (1000-2100 g), and exponential time decay constant for the substrate feed rate (K) (0.6-1.6 h-1) on ethanol production by Saccharomyces cerevisiae grown in sugarcane blackstrap molasses through a fed-batch culture. The highest ethanol productivity (16.9 g/[L x h]) occurred at T = 3 h, K = 1.6 h-1, and M = 1300 g. In addition, productivity was affected by both M (for T = 3 and 4 h) and K (for T = 3 h) and varied inversely with T under any value fixed for M and K. By the quadratic regression multivariable analysis method, equations were determined to estimate ethanol yield and productivity as function of the variables studied (T, K, and M).
D018533:Biomass; D019149:Bioreactors; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D000431:Ethanol