Efficient cross-linking reactions by functional nucleobases capable of in situ activation under neutral conditions.
We have previously described that the oligonucleotides containing phenylsulfide derivatives of 2-amino-6-vinylpurine nucleoside analog are activated within duplex to form cross-link toward cytidine selectively at the target site. However, the cross-linking reaction via the in-situ activation needs acidic conditions. In this paper, we wish to report that the new 2-amino-6-vinylpurine derivatives designed to have a structure of "double-activation" for in-situ activation exhibit fast cross-linking with cytidine under neutral conditions.
D003432:Cross-Linking Reagents / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
D003432:Cross-Linking Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides