Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain.
The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D1 receptor ligand binding could be observed.


CONCLUSIONS
The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism / Q000502:physiology; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D014162:Transfection
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017447:Receptors, Dopamine D1