title: Overproduction and secretion of Bacillus circulans endo-beta-1,3-1,4-glucanase gene (bglBC1) in B. subtilis and B. megaterium.
abstract: A gene coding for endo-beta-1,3-1,4-glucanase (lichenase) containing a recombinant plasmid, pLL200K, was transferred from Bacillus circulans into a new shuttle plasmid, pLLS920, by ligating linearized DNAs of pLL200K and pUB110. B. subtilis RM125 and B. megaterium ATCC14945 transformed with pLLS920 produced the endo-beta-1,3-1,4-glucanase. The enzyme was produced during active growth with maximum activity. The B. subtilis (pLLS920) enzyme was 83 times (8522 mU ml(-1)) more active than that of the gene donor cells (103 mU ml(-1)). The B. megaterium (pLLS920) enzyme was 7 times (735 mU ml(-1)) more active than that of the gene donor cells. While E. coli secreted only about 10% of the produced enzyme, B. subtilis excreted the enzyme completely into the medium and B. megaterium by about 98%. The plasmid pLLS920 was stable in B. megaterium (98%), and in B. subtilis (51%) but not in E. coli (29%).
mesh_terms: D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D001410:Bacillus megaterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001412:Bacillus subtilis / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D044644:Endo-1,3(4)-beta-Glucanase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015202:Protein Engineering / Q000379:methods*; D013045:Species Specificity
chemical_list: D044644:Endo-1,3(4)-beta-Glucanase