title: Effects of electromagnetic fields on proteoglycan metabolism of bovine articular cartilage explants.
abstract: Electromagnetic field (EMF) exposure has been proposed for the treatment of osteoarthritis. In this study, we investigated the effects of EMF (75 Hz, 2,3 mT) on proteoglycan (PG) metabolism of bovine articular cartilage explants cultured in vitro, both under basal conditions and in the presence of interleukin-1beta (IL-1beta) in the culture medium. Proteoglycan synthesis and the residual PG tissue content resulted significantly higher in EMF-exposed explants than in controls, whereas no effect was observed on PG release and nitric oxide (NO) production. IL-1beta induced both a reduction in PG synthesis and an increase in PG release, related to a strong stimulation of NO production, which resulted in a net loss of tissue PG content. In IL-1beta-treated explants, EMF increased PG synthesis, whereas in spite of a slight stimulation of NO production EMF did not modify PG release. This resulted in the residual PG tissue content being maintained at the control level. In both experimental conditions, the effects of EMF were associated with an increase in lactate production. The results of our study show that EMFs are able to promote anabolic activities and PG synthesis in bovine articular cartilage explants. This effect also is maintained in the presence of IL-1beta, thus counteracting the catabolic activity of the cytokine. Altogether, these data suggest that EMF exposure exerts a chondroprotective effect on articular cartilage in vitro.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D002417:Cattle; D046508:Culture Techniques / Q000379:methods; D015536:Down-Regulation / Q000502:physiology / Q000528:radiation effects; D004574:Electromagnetic Fields; D004734:Energy Metabolism / Q000502:physiology / Q000528:radiation effects; D007375:Interleukin-1 / Q000494:pharmacology; D019344:Lactic Acid / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism / Q000528:radiation effects; D010003:Osteoarthritis / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D011509:Proteoglycans / Q000378:metabolism* / Q000528:radiation effects*; D012038:Regeneration / Q000502:physiology / Q000528:radiation effects*; D015854:Up-Regulation / Q000502:physiology / Q000528:radiation effects
chemical_list: D007375:Interleukin-1; D011509:Proteoglycans; D009569:Nitric Oxide; D019344:Lactic Acid