Specific interaction of lectins with liposomes and monolayers bearing neoglycolipids.
The interaction of three lectins (wheat germ, Ulex europaeus I, and Lotus tetragonolobus agglutinins: WGA, UEA-I and LTA) with either N-acetyl-D-glucosamine or L-fucose neoglycolipids incorporated into phospholipid monolayers and liposome bilayers was studied at the air/water interface and in bulk solution. The results show that for both systems studied, synthesized neoglycolipids were capable of binding their specific lectin and that, in general, the binding of lectins increased with the increase in the molar fraction of the saccharide derivative incorporated in either the monolayers or bilayers. However, whereas for UEA-I, molecular recognition was enhanced by a strong hydrophobic interaction, for WGA and LTA successful recognition was predominantly related to the distance between neighboring sugar groups. The observed lengthy adsorption times of these lectins onto their specific ligands were attributed to interfacial conformational changes occurring in the proteins upon their adsorption at the interfaces.
D000117:Acetylglucosamine / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry; D019245:Compressive Strength; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005643:Fucose / Q000737:chemistry; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D037102:Lectins / Q000737:chemistry; D008024:Ligands; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry*; D013499:Surface Properties; D013500:Surface Tension; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D002413:Cations, Divalent; D006017:Glycolipids; D037102:Lectins; D008024:Ligands; D008081:Liposomes; D010743:Phospholipids; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; C027112:fucose-binding lectin; D005643:Fucose; D004134:Dimyristoylphosphatidylcholine; D000117:Acetylglucosamine