Quantitative determination of anti-fungal and insecticide amides in adult plants, plantlets and callus from Piper tuberculatum by reverse-phase high-performance liquid chromatography.
A rapid, sensitive and reliable reverse-phase HPLC method was used for the quantitative determination of the anti-fungal and insecticide amides, dihydropiplartine (1), piplartine (2), deltaalpha,beta-dihydropiperine (3) and pellitorine (4) in plants in natura, in plantlets in vitro and ex vitro, and in callus of Piper tuberculatum. Well-resolved peaks were obtained with good detection response and linearity in the range of 15.0-3000 microg/mL. The plants in natura contained compounds 1-4, the plantlets ex vitro and in vitro accumulated compounds 1-2 and 1-4, respectively, while only amide 4 was found in callus.
D000577:Amides / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D046508:Culture Techniques; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D015394:Molecular Structure; D031701:Piper / Q000737:chemistry* / Q000254:growth & development; D010880:Piperidines / Q000032:analysis*; D010881:Piperidones / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D053284:Polyunsaturated Alkamides; D013057:Spectrum Analysis
D000577:Amides; D000935:Antifungal Agents; D005231:Fatty Acids, Unsaturated; D007306:Insecticides; D010880:Piperidines; D010881:Piperidones; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; C479505:deltaalpha,beta-dihydropiperine; C479507:dihydropiplartine; C498077:piperlongumine; C008778:pellitorine