title: Evidence for a persistent Na-conductance in identified command neurones of the snail, Helix pomatia.
abstract: Neurones RPa3 and LPa3 were identified as 'command' neurones in the Helix parietal ganglia. The physiological role of these cells is the integration of sensory information before triggering withdrawal behaviour. Properties of the Na-channels are poorly understood in these neurones which produce Na(+)-dependent action potentials in Ca(2+)-free solution. Our aim was to describe the kinetic properties and TTX-sensitivity of the Na-channels of these cells, and to provide evidence for the existence of a persistent inward sodium current (I(NaP)) in them. Two-microelectrode voltage- and patch-clamp techniques were used on isolated or semi-isolated neurones. The kinetics and potential dependence of the transient inward sodium current (I(NaT)) agreed well with those obtained on other molluscan neurones. We concluded that I(NaT) present in these neurones is slow and TTX-resistant (k(D)=8 microM of TTX) and has two components with different rates of inactivation. In addition, the presence of an I(NaP) component was revealed. We showed that I(NaP) is neither an artifact nor the contribution of a Ca-channel or a 'window' current. With slow voltage ramp pulses I(NaP) could be activated and separated from I(NaT). Like I(NaT) it appeared to be TTX-resistant and Na-dependent. I(NaP) was upregulated by increased pH (8.0) and decreased by elevated extracellular Mg(2+) concentration parallel with the I(NaT). Our results suggest that I(NaP) originates from the same set of sodium channels that underlie I(NaT).
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D006372:Helix, Snails / Q000502:physiology*; D015640:Ion Channel Gating / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology*
chemical_list: D015222:Sodium Channels