title: A mutation in the gene involved in sister chromatid separation causes a defect in nuclear mRNA export in fission yeast.
abstract: Fission yeast ptr4-1 is one of the mRNA transport mutants that accumulate poly(A)(+) RNA in the nuclei at the nonpermissive temperature. We cloned the ptr4(+) gene and found that it is identical with the cut1(+) gene essential for chromosome segregation during mitosis. ptr4/cut1 has no defects in nucleocytoplasmic transport of a protein, indicative of a specific blockage of mRNA export by this mutation. A mutant of Cut2p cooperating with Cut1p in sister chromatid separation also showed defective mRNA export at the nonpermissive temperature. Our results suggest a novel linkage between the cell division cycle and nuclear mRNA export in eukaryotic cells.
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002842:Chromatids; D017404:In Situ Hybridization, Fluorescence; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger