An immuno-affinity method for the purification of mannose 6-phosphate receptor proteins.
In a recent study, we have developed an ELISA method to quantify the mannose 6-phosphate receptor (MPR) proteins [J. Biochem. Biophys. Methods 52 (2002) 111]. In the present study, we have used the goat MPR 300 antibody and peptide specific antibodies to human MPR 46 to develop simple and efficient immuno-affinity matrices, which can be used to purify the MPR proteins from goat liver in a single step. The identity of the immuno-affinity purified receptors is confirmed by their molecular masses as well as by their immunoreactivity.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002846:Chromatography, Affinity / Q000379:methods*; D006041:Goats; D006801:Humans; D011233:Precipitin Tests / Q000379:methods; D017527:Receptor, IGF Type 2 / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D017527:Receptor, IGF Type 2