title: A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium.
abstract: A new enzymatic route for biodiesel production from soybean oil was developed using methyl acetate as a novel acyl acceptor. Novozym 435 (immobilized Candida antarctica lipase) gave the highest methyl ester (ME) yield of 92%. The optimum conditions of the transesterification were 30% enzyme based on oil weight; a molar ratio of methyl acetate/oil of 12:1; temperature 40 degrees C and reaction time 10 h. Since no glycerol was produced in the process, this method is very convenient for recycling the catalyst and by-product triacetylglycerol showed no negative effect on the fuel property.
mesh_terms: D000085:Acetates / Q000737:chemistry*; D003247:Conservation of Natural Resources; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins; D005742:Gasoline; D008049:Lipase / Q000737:chemistry*; D010865:Pilot Projects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013024:Soybean Oil / Q000737:chemistry*; D013696:Temperature
chemical_list: D000085:Acetates; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005742:Gasoline; D013024:Soybean Oil; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase; C046923:methyl acetate