Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system.
Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in 1/4 strength Murashige and Skoog medium (1/4 MS) plus 30 gr x L(-1) sucrose and supplemented with 4.4 microM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr x L(-1) sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in 1/4 MS (30 gr x L(-1) sucrose, 0.25% Phytagel) with 7.3 microM IBA and 2) 21 days in the same medium without IBA and 20 microM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation schedule can be implemented in breeding programs of Ilex dumosa.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology*; D003583:Cytokinins / Q000494:pharmacology; D030017:Ilex / Q000187:drug effects / Q000254:growth & development*; D007701:Kinetin; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011687:Purines; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology
D001593:Benzyl Compounds; D003470:Culture Media; D003583:Cytokinins; D010937:Plant Growth Regulators; D011687:Purines; D013395:Sucrose; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin