The improvement of monitoring methods of cattle infection with bovine leukemia virus (BLV).
This study demonstrates the usefulness of PLA and nested-PCR methods for the detection of cattle infection with enzootic bovine leukemia virus. Serological control with PLA was by 1.7% more sensitive than reference ELISA examination. Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D004279:DNA, Viral / Q000097:blood; D003612:Dairying; D016583:Enzootic Bovine Leukosis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000592:standards / Q000662:veterinary*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D004279:DNA, Viral