Metoclopramide protection of cholinesterase from paraoxon inhibition.
This study evaluated the protective effect of the benzamide compound metoclopramide (MCP) against inhibition by paraoxon (POX) as assessed by red blood cell acetylcholinesterase (RBC-AChE) activity. Three groups of 6 rats each were used. All substances were applied ip daily for 5 d, followed by a 2-d rest. The 7-d cycle was repeated 6 times. Group 1 received 100 nM POX, Group 2 received 50 microM MCP. Group 3 received 100 nM POX + 50 microM MCP. Red blood cell acetylcholinesterase measurements were performed at base line and then after each 7-d cycle. Enzyme activities were compared using the Mann-Whitney rank order test. Metoclopramide conferred significant in vivo protection from inhibition of RBC-AChE by POX.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology*; D010261:Paraoxon / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats
D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D000110:Acetylcholinesterase; D008787:Metoclopramide; D010261:Paraoxon