Mass spectrometric method for detecting carbon 13 enrichment introduced by folate coenzymes in uric acid.
Using [2-13C]uric acid as a test material, we developed a mass spectrometric procedure that detects and estimates the difference in 13C enrichment at the positions of carbons 2 and 8 of the purine ring. This method could replace radiochemical methods and could trace the incorporation of carbon fragments into the purine ring from 13C-labeled metabolites in humans.
D002247:Carbon Isotopes / Q000032:analysis*; D003067:Coenzymes / Q000737:chemistry* / Q000652:urine; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D014527:Uric Acid / Q000737:chemistry* / Q000652:urine
D002247:Carbon Isotopes; D003067:Coenzymes; D014527:Uric Acid; D005492:Folic Acid