title: Molecular cloning and characterization of a cDNA encoding cytochrome c oxidase subunit Va from the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae).
abstract: A cDNA encoding subunit Va of cytochrome c oxidase (EC 1.9.3.1) was cloned and characterized from a lesser grain borer (Rhyzopertha dominica) cDNA library. The complete cDNA consists of 693-bp and contains an open reading frame of 450-bp that encodes 150 amino acid residues. The sequence includes a 28-bp putative N-terminal and a 122-bp putative mature protein. The estimated molecular weight and pI for the predicted mature protein are 13,962 and 4.60, respectively. The cDNA-deduced amino acid sequence of the mature protein shows 73% identity to that of a corresponding subunit of African malaria mosquito (Anopheles gambiae) and 59% identity to that of the fruit fly (Drosophila melanogaster). In addition, 31% of all amino acid residues are conserved among six different animal species. Evolutionary distance analysis suggests that cytochrome c oxidase subunit Va from R. dominica is most similar to the corresponding subunit from the malaria mosquito. Northern analysis revealed a single 4.9-kb transcript that is much larger than that found in mammalian species.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics*; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D021122:Protein Subunits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D021122:Protein Subunits; D003576:Electron Transport Complex IV