Biotransformation of [ring-U-14C]4-n-nonylphenol by Agrostemma githago cell culture in a two-liquid-phase system.
The biotransformation of [14C]4-n-nonylphenol (5 mg l(-1); 10 mg l(-1)) by Agrostemma githago cell suspensions was studied using a batch two-liquid-phase system (medium/n-hexadecane 200:1, v/v). The highly lipophilic 4-n-nonylphenol was applied via n-hexadecane phase. After 7 d of incubation, more than 85% of applied 4-n-nonylphenol was absorbed by the cells, and 40% was transformed to 10 side-chain monohydroxylated metabolites (two with additional double bond at side-chain). The primary metabolites were analyzed by GC-EIMS. In the cells, the monohydroxylated products and residual 4-n-nonylphenol were present as glycosides. The method proved to be suitable for the production of primary metabolites of 4-n-nonylphenol on a larger scale for identification purposes and for metabolic profiling of the compound.
D001711:Biotransformation; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste / Q000517:prevention & control; D010636:Phenols / Q000378:metabolism*; D027842:Syzygium / Q000254:growth & development* / Q000378:metabolism*
D002250:Carbon Radioisotopes; D007220:Industrial Waste; D010636:Phenols; C025256:nonylphenol