Lymph drainage in the human dental pulp.
We investigated which structural components are responsible for maintaining interstitial fluid equilibrium in the pulpal tissue, for which the existence of an effective lymph drainage is postulated. There have been only a small number of investigations on pulpal lymph tissue. Therefore, we decided to perform a detailed structural analysis. Twenty vital, healthy teeth that had to be extracted for orthodontic reasons were immersed in Patent Blue for 10 to 15 minutes after opening the pulpal cavity. They were then extracted and the dental pulps were opened by cleavage of the surrounding hard tooth structure. Subsequently, the specimens were prepared for light and electron microscopic investigation. A clear blue ring of stain was detected by light microscopy in Weil's zone in the coronal region of the pulp, the cell-rarefied layer surrounded by the odontoblasts. No dye deposition was observed in the apical part. However, using transmission electron microscopy, capillary structures with typical morphological characteristics of lymphatic vessels were found apically. The coronal part of the pulp did not reveal any such vascular structures. It may be concluded from these findings that the lymph in the coronal region is collected in interstitial tissue clefts and drained towards the apex, whence it is further transported via lymph capillaries.
D000328:Adult; D002196:Capillaries / Q000033:anatomy & histology; D003782:Dental Pulp / Q000033:anatomy & histology / Q000098:blood supply* / Q000648:ultrastructure; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure; D008208:Lymphatic System / Q000033:anatomy & histology* / Q000098:blood supply / Q000000981:diagnostic imaging; D008854:Microscopy, Electron; D008875:Middle Aged; D014463:Ultrasonography