[Revitalizing donor livers after cardiovascular arrest with venous oxygen persufflation].
The experimental study investigates the resuscitation of livers procured from non heart beating donors by venous systemic oxygen persufflation in a pig transplantation model. In group 1 livers were harvested from donor animals after 60 min of potassium induced cardiac arrest. Livers were then flushed and preserved in 4 degrees C cold University of Wisconsin solution for 4 hrs. Then liver transplantation was performed in recipient animals. Animals of group 2 received the same treatment, but during hypothermic storage we applied venous systemic oxygen gas persufflation (VSOP) via the supra-hepatic vena cava. Livers of group 3 were harvested without warm ischemia and without oxygen treatment during cold ischemic storage and were then transplanted. All recipient animals of group 2 and 3 survived the five day follow-up time. All recipient animals of group 1 died a few hours after liver reperfusion due to circulatory shock or liver failure. The liver quality of group 2 recipient animals was similar to group 3 livers, because serum transaminase levels and blood coagulation test showed no significantly different values between those groups. Our data suggest that livers procured from non heart beating donors after 1 hr of cardiac arrest can be resuscitated and successfully transplanted by therapeutic treatment with VSOP.
D000818:Animals; D005260:Female; D006085:Graft Survival / Q000502:physiology; D006323:Heart Arrest / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion; D015427:Reperfusion Injury / Q000503:physiopathology*; D013552:Swine; D014019:Tissue Donors; D014683:Vena Cava, Superior
D010100:Oxygen