Specificity of one-base mismatch detection with MagiProbe.
MagiProbe have been demonstrated to be a homogeneous type of probe that emits fluorescence upon hybridization and with specificity for one-base mismatch. To obtain more comprehensive information for the properties of mismatch recognition, examination of mismatch recognition of MagiProbe for the eight single-base mismatches in variety of sequence contexts was performed. The results indicate that the recognition of base mismatches is influenced predominantly by combination of mispaired bases.
D020137:Base Pair Mismatch; D001483:Base Sequence; D015335:Molecular Probes; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D015335:Molecular Probes