Study on DNA precipitation with a cationic polymer PAC (poly aluminuim chloride).
We studied on the interactions between DNA and polyaluminuimchloride (PAC) by ultraviolet spectroscopy and electron microscopy to understand the interaction between negative-charged macromolecules and PAC. Sodium ion seems to have only weak effect on the manner of DNA/PAC complex. However, acetate and phosphate anions competed against phosphate in DNA for interaction with PAC. It seems that negative macromolecule in a water system may be similar behavior with DNA under PAC.
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D002412:Cations; D011232:Chemical Precipitation; D002712:Chlorides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron
D017607:Aluminum Compounds; D002412:Cations; D002712:Chlorides; D000077410:Aluminum Chloride; D004247:DNA