Synthesis of double-headed 2-5A-antisense chimeras and their ability to activate human RNase L.
The synthesis of a novel 2-5A-antisense chimera having two molecules of 2-5A tetramer at the 5'-terminus of the antisense moiety with an ethylene glycol linker is described. The ability of the synthesized 2-5A antisense chimeras to activate RNase L was estimated by monitoring the cleavage of a target RNA by the activated RNase L. It was found that the double-headed 2-5A-antisense chimera linked with two molecules of a butanediol linker more efficiently cleaved the target RNA as compared with the single-headed 2-5A-antisense chimera and the double-headed 2-5A-antisense chimera linked with a molecule of the butanediol linker.
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000494:pharmacology
D016376:Oligonucleotides, Antisense; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease