[Experimental study on effect of gusongbao contained serum of old rats on osteoblast proliferation of rabbits].
OBJECTIVE
To observe the effect of Gusongbao (GSB) on proliferation and metabolism of osteoblast cultured in vitro.


METHODS
Old rats, aged 18 months, were given GSB 1.5 g/kg, twice a day for 3 days by intragastric perfusion. Blood of the rats was collected 1 hr after the final perfusion to isolate serum for preparing, with D8900 medium, the culture media containing 7.5% or 15% GSB, which was used to culture osteoblast for 24 hrs. Besides, D8900 media containing 7.5% or 15% old rats'serum without medication, containing 20 mumol/L, sodium fluoride, and simple D8900 medium were prepared for control. The cell proliferation was detected by MTT method, and the changes of Ca2+ concentration and ALP content in supernatant of culture were also observed.


RESULTS
The osteoblast proliferation cultured in GSB serum containing medium was significantly increased than those cultured in the other control media (P < 0.01), at the same time, the Ca2+ consumption increased and the ALP content elevated significantly (P < 0.05, P < 0.01).


CONCLUSIONS
GSB could promote the DNA synthesis, increase the utilization of Ca2+ and accelerate the growth and proliferation of osteoblast.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010024:Osteoporosis / Q000473:pathology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D004247:DNA; D002118:Calcium