[Pharmacokinetics of the alkaloids from the processed seeds of Strychnos nux-vomica in rats].
OBJECTIVE
To study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of Strychnos nux-vomica with RP-HPLC method.


METHODS
Hypersil BDS C18 column was used and the mobile phase consisted of acetonitrile-water at the flow rate of 0.8 mL.min-1. The UV detection wave length was 254 nm.


RESULTS
The concentration-time data of strychnine, brucine, strychnine N-oxide and brucine N-oxide were all in accordance with an open two-compartment model after i.v. alkaloids. Their parameters were as follows: T1/2 alpha were (8 +/- 5), (4 +/- 3), (6.2 +/- 1.7) and (3.0 +/- 0.8) min, T1/2 beta were (262 +/- 125), (416 +/- 131), (285 +/- 50) and (342 +/- 141) min, CL were (17 +/- 4), (21 +/- 12), (1.9 +/- 1.8) and (2.8 +/- 1.1) mL.min-1, Vc were (1.4 +/- 0.5), (1.7 +/- 1.1), (0.24 +/- 0.16) and (0.23 +/- 0.06) L.kg-1, Vd were (6.0 +/- 1.2), (12 +/- 7), (0.8 +/- 0.6) and (1.5 +/- 0.6) L.kg-1, AUC were (57,578 +/- 25,578), (35,240 +/- 15,616), (93,088 +/- 22,375) and (177,712 +/- 120,110) h.microgram.L-1, respectively.


CONCLUSIONS
The method is a good reference for pharmacokinetics in human bodies.
D000470:Alkaloids / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D003497:Cyclic N-Oxides / Q000302:isolation & purification / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005260:Female; D006358:Hot Temperature; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D013331:Strychnine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000493:pharmacokinetics; D031461:Strychnos nux-vomica / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
D000470:Alkaloids; D003497:Cyclic N-Oxides; D004365:Drugs, Chinese Herbal; C083067:brucine N-oxide; C083806:brucine; C083068:strychnine N-oxide; D013331:Strychnine