Identification and characterization of mammalian 5-formyluracil-DNA glycosylase.
5-Formyluracil is a major oxidative thymine lesion with mutagenic and cytotoxic properties. In this study, we have partially purified and characterized a mammalian 5-formyluracil-DNA glycosylase (FDG) from rat liver. FDG was a monofunctional DNA glycosylase and removed 5-formyluracil, uracil, 5-hydroxyuracil, 5-hydroxylmethyluracil in single-stranded and double-stranded DNA. Several lines of evidence indicate that FDG is a rat SMUG1 homologue. Human SMUG1 also exhibited similar enzymatic properties.
D000818:Animals; D045647:DNA Glycosylases; D006801:Humans; D008099:Liver / Q000201:enzymology; D009699:N-Glycosyl Hydrolases / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism
C007777:5-formyluracil; D014498:Uracil; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases