title: Components in the inoculum determine the kinetics of Azotobacter vinelandii cultures and the molecular weight of its alginate.
abstract: In cultures of Azotobacter vinelandii inoculated using washed cells (avoiding exhausted broth components) alginates of a higher molecular weight (1200 kDa) than those obtained in cultures conventionally inoculated (350 kDa), were produced. Also, when comparing conventionally inoculated cultures with those inoculated with washed-cells, the alginate lyase activity was delayed and the final polymer concentration decreased from 4.8 to 3.5 g l(-1). This suggests that components in the exhausted inoculum broth play important regulatory roles in alginate biosynthesis and needs to be taken into account when describing polymer biosynthesis.
mesh_terms: D000464:Alginates / Q000737:chemistry*; D016948:Azotobacter vinelandii / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D020723:Glucuronic Acid / Q000096:biosynthesis* / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D007700:Kinetics; D008970:Molecular Weight; D014783:Viscosity
chemical_list: D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D020723:Glucuronic Acid