[Skin tissue engineering].
Cultivated epithelial autografts as multilayered, thin sheets represent a common standard in clinically applied tissue engineering substitutes, outnumbering all experimental alternatives. However, the unsatisfying short- and long-term results concerning mechanical stability and scarring require alternatives. The cultivation and transplantation of cultured autologous keratinocytes as a single cell suspension in a fibrin matrix, combined with allogenic skin grafting, has been investigated extensively in athymic nude mice. Wounds can be reliably reepithelialized after a cultivation period of only 14 days. Moreover, the successful combination of keratinocyte fibrin suspension and acellular dermis in an attempt to regenerate full thickness skin defects in a pig model has been demonstrated. The usefulness of subconfluently cultured keratinocytes-which can be harvested very early and are easy to handle-is enhanced by cotransplantation with decellularized dermis.
D000818:Animals; D002478:Cells, Cultured; D020405:Dermis / Q000637:transplantation; D004817:Epidermis / Q000637:transplantation; D015718:Fibrin Tissue Adhesive; D015316:Genetic Therapy; D006133:Growth Substances; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering
D015718:Fibrin Tissue Adhesive; D006133:Growth Substances