Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex.
The nuclear-transport protein importin-beta mediates the nuclear import of the transcription factor SREBP-2 without requiring adaptor proteins such as importin-alpha. An importin-beta-SREBP-2 HLHZ domain complex was purified and crystallized. The crystals belong to space group P2(1)2(1)2(1) and show diffraction to at least 3.0 A resolution. The unit-cell parameters are a = 101.0, b = 113.2, c = 240.0 A. Structure determination using the MAD or SAD method is under way.
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D051379:Mice; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D051782:Sterol Regulatory Element Binding Protein 2; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D028961:beta Karyopherins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics
D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; C497390:SREBF2 protein, human; C497391:Srebf2 protein, mouse; D051782:Sterol Regulatory Element Binding Protein 2; D014157:Transcription Factors; D028961:beta Karyopherins