title: The thioltransferase (glutaredoxin) 1 gene of fission yeast is regulated by Atf1 and Pap1.
abstract: We previously isolated a gene encoding thioltransferase (TTase1) from the fission yeast Schizosaccharomyces pombe. Using a TTase-lacZ fusion plasmid, carrying a 666 bp region upstream of the translation initiation point, we found that expression of TTase1 was enhanced by metal ions, diamide and NO-generating S-nitroso-N-acetylpenicillamine (SNAP). In the present work, we examined the regulation of TTase1 expression using a series of deletion mutants and identified a negatively acting sequence between bp -469 and -339. Atf1 is required for basal expression of TTase1, and Pap1 is required for its inducible expression by mercuric chloride, diamide and SNAP. The -469 approximately -339 bp region is also responsible for mediating the inducible expression.
mesh_terms: D051697:Activating Transcription Factor 1; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003958:Diamide / Q000378:metabolism; D004351:Drug Resistance / Q000502:physiology; D004791:Enzyme Inhibitors / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D054477:Glutaredoxins; D008627:Mercuric Chloride / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D026423:S-Nitroso-N-Acetylpenicillamine / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D051697:Activating Transcription Factor 1; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D054477:Glutaredoxins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; D010750:Phosphoproteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D013439:Sulfhydryl Reagents; C106740:atf1 protein, S pombe; D003958:Diamide; D008627:Mercuric Chloride; D026423:S-Nitroso-N-Acetylpenicillamine; D010088:Oxidoreductases; D001616:beta-Galactosidase