The epidemiology of Staphylococcus aureus infections from subclinical mastitis in dairy cows during a control programme.
The objective was to investigate the spread of S. aureus in seven dairy herds. Milk samples were taken to determine mastitis pathogens and somatic cell count. An approved hygiene programme was established to control the spread of S. aureus from quarter to quarter. S. aureus isolates were differentiated by geno- and phenotyping to trace their spread within a herd. In two herds S. aureus showed a high prevalence, but were eliminated from the herds by the control programme. In these herds and a third herd typing results identified a particular type that was found much more frequently than all other types. The frequent types were repeatedly detectable during the study. In three other herds with a lower S. aureus prevalence and also in one herd with a long history of S. aureus and high prevalence subclinical mastitis was caused by several distinguishable S. aureus types. These types occurred simultaneously in the particular herd and showed little or no tendency to spread from quarter to quarter. They seemed to circumvent the control procedures resulting in a relatively high rate of new infected animals and therefore were hardly eliminated. The typing results and the clinical observations indicated that strains differed in their tendency to spread and their ability to infect udder quarters. In three herds typing methods identified a predominant type with the common epidemiological features of a contagious mastitis pathogen, while in the other herds the S. aureus type patterns were similar to that of environmental pathogens.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D005260:Female; D005838:Genotype; D015994:Incidence; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000635:transmission; D008892:Milk / Q000166:cytology / Q000382:microbiology; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*