[Proteolysis but not ICAM-1 expression in fatty liver increased in tissue harvesting--role of Kupffer cells].
The analysis of amino acids in perfusion effluents in experimental liver transplantation is a valid parameter of organ viability at organ harvest. Depletion of Kupffer cells with gadolinium chloride (GdCl3) in donor animals prevents primary nonfunction of fatty livers after transplantation, diminishes amino acid release at harvest, but blocks the increased expression of the adhesion molecule ICAM-1 only after transplantation. Thus, the increased proteolysis in marginal donor livers is not induced by cytokines, but kupffer cell dependent.
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D005235:Fatty Liver, Alcoholic / Q000473:pathology / Q000503:physiopathology*; D005682:Gadolinium / Q000494:pharmacology; D015870:Gene Expression / Q000502:physiology; D006085:Graft Survival / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D007728:Kupffer Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D007958:Leukocyte Count; D008111:Liver Function Tests; D016031:Liver Transplantation; D010447:Peptide Hydrolases / Q000502:physiology*; D051381:Rats; D020858:Tissue and Organ Harvesting
D000596:Amino Acids; D018799:Intercellular Adhesion Molecule-1; D005682:Gadolinium; D001219:Aspartate Aminotransferases; D010447:Peptide Hydrolases; C038958:gadolinium chloride