Adenovirus-mediated gene transfer of Bcl-xL impedes neurite regeneration in vitro.
Mouse retinal explants were transfected with recombinant adenovirus vector carrying the green fluorescent protein (GFP) gene and the rat bcl-xL gene (Adeno-Bcl-xL) to determine its ability to protect retinal ganglion cells against apoptotic cell death and to promote retinal ganglion cell neurite regeneration. Adeno-Bcl-xL-incubated retinas had reduced apoptosis compared to controls. However, neurite regeneration in adeno-treated retinas was less than that of vector-free retina. These results suggest that the usefulness of adenovirus vectors for gene therapy for retinal ganglion cells may be limited.
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000235:genetics*; D016501:Neurites / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000008:administration & dosage* / Q000235:genetics*; D051381:Rats; D012160:Retina / Q000502:physiology; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; C494813:Bcl2l1 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein