Expression of the gene coding for bacterial hemoglobin improves beta-galactosidase production in a recombinant Pichia pastoris.
Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidase activity to 4-fold higher than that of VHb-free cell in yeast extract/peptone/methanol medium under aerobic cultivation.
D000332:Aerobiosis / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D018014:Gene Transfer Techniques; D024861:Genetic Enhancement / Q000379:methods; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism*; D000074435:Peroxisome-Targeting Signal 1 Receptor; D010843:Pichia / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D054793:Truncated Hemoglobins; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics*
D001426:Bacterial Proteins; D006454:Hemoglobins; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D001616:beta-Galactosidase