Directed evolution of xylanase J from alkaliphilic Bacillus sp. strain 41M-1: restore of alkaliphily of a mutant with an acidic pH optimum.
Alkaliphilic Bacillus sp. strain 41M-1 produces an alkaliphilic xylanase (xylanase J). The newly constructed mutant E177Q deltaJC had an acidic pH optimum and showed almost no activity at pH 8.0. The alkaliphily of the enzyme was restored by directed evolution. The evolved mutants, Y176S/E177Q deltaJC and G32V/Y176D/E177Q deltaJC, retained about 30% and 43% activity of their maximal activities at pH 6.0, respectively.
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D019020:Directed Molecular Evolution; D043364:Endo-1,4-beta Xylanases; D016153:Genes, Suppressor; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism
D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases