Identification of a novel DNA methyltransferase activity from Bacillus thuringiensis.
A DNA methyltransferase activity was identified in a strain of Bacillus thuringiensis that was found to protect DNA from cleavage by the restriction endonuclease HaeIII at overlapping sites. Site-directed mutagenesis was used to confirm therecognition sequence of the methyltransferase as ACGGC.
D001413:Bacillus thuringiensis / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*
D004269:DNA, Bacterial; D008780:Methyltransferases; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases