title: The AF-6 homolog canoe acts as a Rap1 effector during dorsal closure of the Drosophila embryo.
abstract: Rap1 belongs to the highly conserved Ras subfamily of small GTPases. In Drosophila, Rap1 plays a critical role in many different morphogenetic processes, but the molecular mechanisms executing its function are unknown. Here, we demonstrate that Canoe (Cno), the Drosophila homolog of mammalian junctional protein AF-6, acts as an effector of Rap1 in vivo. Cno binds to the activated form of Rap1 in a yeast two-hybrid assay, the two molecules colocalize to the adherens junction, and they display very similar phenotypes in embryonic dorsal closure (DC), a process that relies on the elongation and migration of epithelial cell sheets. Genetic interaction experiments show that Rap1 and Cno act in the same molecular pathway during DC and that the function of both molecules in DC depends on their ability to interact. We further show that Rap1 acts upstream of Cno, but that Rap1, unlike Cno, is not involved in the stimulation of JNK pathway activity, indicating that Cno has both a Rap1-dependent and a Rap1-independent function in the DC process.
mesh_terms: D000818:Animals; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D020827:rap1 GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; C092747:cno protein, Drosophila; D011505:Protein-Tyrosine Kinases; D009218:Myosins; D016547:Kinesins; D020827:rap1 GTP-Binding Proteins