title: Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth.
abstract: The purpose of this investigation was to compare the antimicrobial effect of MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) with that of NaOCl with and without EDTA. Eighty-five extracted human teeth were contaminated with Enterococcus faecalis for 4 weeks. After biomechanical instrumentation using 1.3% or 5.25% NaOCl as root canal irrigant, the root canal and the external surface of each tooth were exposed to a 5-min application of MTAD, 1.3% NaOCl, 5.25% NaOCl or a 1-min application of EDTA followed by irrigation with 5 ml of 1.3% NaOCl or 5.25% NaOCl. Teeth or dentin shavings were cultured to determine presence or absence of the test bacteria. Fisher's exact test showed that the combination of 1.3% NaOCl as a root canal irrigant and MTAD as a final rinse was significantly more effective against E. faecalis than the other regimens. The chi2 test showed no difference between the other regimens.
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002614:Chelating Agents / Q000627:therapeutic use; D016009:Chi-Square Distribution; D019343:Citric Acid / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology; D004202:Disinfectants / Q000627:therapeutic use; D004318:Doxycycline / Q000627:therapeutic use*; D004492:Edetic Acid / Q000627:therapeutic use; D013293:Enterococcus faecalis / Q000187:drug effects*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000628:therapy*; D006801:Humans; D011136:Polysorbates / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation; D016037:Single-Blind Method; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013501:Surface-Active Agents / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D004202:Disinfectants; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D013501:Surface-Active Agents; D019343:Citric Acid; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D004318:Doxycycline