Survival of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella in juice concentrates.
The survival of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella was studied in apple, orange, pineapple, and white grape juice concentrates and banana puree. Pouches of juice concentrate or puree were inoculated with pathogens at a level > or = 10(3) CFU/g and stored at -23 degrees C (-10 degrees F). Pathogen survival was monitored at 6 and 24 h, once a week for four consecutive weeks, and biweekly thereafter until 12 weeks. When pathogens were not detectable by direct plating, samples were enriched in universal preenrichment broth for 72 h and plated on selective media. Results showed that E. coli O157:H7, L. monocytogenes, and Salmonella were recoverable from all five concentrates through 12 weeks of storage at -23 degrees C.
D001628:Beverages / Q000382:microbiology*; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000254:growth & development*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation; D005638:Fruit; D008089:Listeria monocytogenes / Q000254:growth & development*; D012475:Salmonella / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors