Interaction of sodium nitroprusside/liposome system with bovine serum albumin: a short-cut determination.
A rapid determination of protein-liposome binding was developed to predict the circulation time of the system within an animal, which is a function of the amount and type of protein bound. The binding pattern of albumin to liposomes, with and without sodium nitroprusside (SNP), was analyzed by SDS-PAGE. Liposomes were made of egg yolk lecithin, soybean lecithin and dimyristoyl lecithin, and contained SNP. They bound 58%, 26% and 100% bovine serum albumin, respectively, when compared to their corresponding controls lacking SNP. The method applied is simpler and significantly faster than ordinary chemical determinations.
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D009599:Nitroprusside / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D008081:Liposomes; D046911:Macromolecular Substances; D010713:Phosphatidylcholines; D009599:Nitroprusside; D012710:Serum Albumin, Bovine