[Effect of hypothermic preservation on ICAM-1 mRNA induction, leukocyte-endothelium interaction and microvascular perfusion after pancreas transplantation in the rat].
A prolonged period of 18 h hypothermic preservation in pancreas grafts led to a significant induction of ICAM-1 mRNA, enhanced leukocyte-adherence/-extravasation and compromised microvascular perfusion upon 2 h of reperfusion when compared to a 2 h preservation period. Therefore, during the period of prolonged cold ischemia changes within the graft must take place causing a reinforced ICAM-1 mRNA-induction already early after the onset of reperfusion. These results underline the relevance of the ischemia/reperfusion injury for the development of complications arising in the early course following pancreas transplantation.
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D003080:Cold Temperature; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005786:Gene Expression Regulation / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D007962:Leukocytes / Q000502:physiology*; D008833:Microcirculation / Q000503:physiopathology; D009926:Organ Preservation / Q000379:methods*; D010179:Pancreas / Q000098:blood supply*; D016035:Pancreas Transplantation / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000235:genetics* / Q000503:physiopathology
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1