Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening.
An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target.
D000818:Animals; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D014227:Triazines / Q000737:chemistry*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
D014227:Triazines