NBS-DMSO as a nonaqueous nonbasic oxidation reagent for the synthesis of oligonucleotides.
A new method for the oxidation of nucleoside phosphite triester into phosphate triester under nonbasic and nonaqueous conditions using NBS-DMSO in CH(3)CN has been developed. The utility of this method for solution- and solid-phase synthesis of oligonucleotide is demonstrated.
D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010084:Oxidation-Reduction
D007202:Indicators and Reagents; D009841:Oligonucleotides; D004121:Dimethyl Sulfoxide