Pressure-controlled on-column injection method for microcolumn liquid chromatography.
A pressure-controlled on-column injection method was developed for microcolumn liquid chromatography. The system was assembled from a syringe pump, a Model M-445 Six-Way Micro Selection Valve, a separation column and a UV detector. The injection volume could be regulated by changing the applied pressure and/or the sample loading time. The system was evaluated in the ion-exchange mode. The system was applied to the determination of anions in river-water samples.