2,6-Diaminonaphthyridine derivatives bind to a single nucleotide bulge in DNA.
2,6-Diamino-1,8-naphthyridine derivative (daNpt), which possesses hydrogen bonding groups in an alignment of donor-acceptor-acceptor-donor binds to a single nucleotide bulge in the duplex. The melting temperatures (Tm) of all duplexes containing a bulge were increased, especially for the cytosine (C) and thymine (T) bulges, in the presence of daNpt, whereas only a small increase of Tm was observed for the fully matched duplexes. It was suggested by pH dependency of Tm and UV spectra, that a protonation of daNpt should play an important role for the recognition of C and T bulges.
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009287:Naphthyridines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D009287:Naphthyridines; D004247:DNA