title: Early identification of bacteria leading to central venous catheter contamination.
abstract: Catheter-related bloodstream infections (CRBSI) are a common problem in patients after central venous catheterization. Using DNA analysis we compared bacteria found on the tip of central venous catheters removed because of clinical signs of CRBSI with bacteria found on needle, dilator, and guidewire used for insertion of these catheters. In five of seven central venous catheters removed because of clinical signs of CRBSI, bacteria on the catheter tip were genetically identical to bacteria found on the insertion device, proving that catheter contamination in these cases was caused by contacting bacteria during the initial puncture. These findings may be important for antibiotic prophylaxis or therapy in patients at risk for CRBSI.
  
  
  CONCLUSIONS
  In five of seven central venous catheters removed because of clinical signs of catheter-related blood infections, DNA analysis showed bacteria found on the catheter tip to be identical with bacteria found on the puncture kits used for insertion of these catheters.
mesh_terms: D001424:Bacterial Infections / Q000209:etiology / Q000382:microbiology*; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002876:Chromosomes, Bacterial / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D009339:Needles / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk
chemical_list: D004269:DNA, Bacterial