Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis.
OBJECTIVE
To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA).


METHODS
Controlled experimental study.


METHODS
The animal laboratory of the Experimental Surgery Center of Ege University, Izmir, Turkey.


METHODS
Ten nondiabetic (control) and 20 diabetic adult male albino rats.


METHODS
The rats were IP injected with 40 mg/kg streptozotocin for 5 days to create a diabetic condition that was confirmed by testing blood glucose levels 5-7 days after the first injection.


METHODS
Mean testicular diameter, mean semniferous tubuli diameter (MSTD), and PCNA index.


RESULTS
Six weeks after the streptozotocin injection, both the mean testicular and the seminiferous tubuli diameters were significantly decreased in diabetic rats compared with the control group.


CONCLUSIONS
The effects of diabetes on spermatogenesis can be clearly detected as a decrease in testicular diameter, MSTD, and PCNA index.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D012671:Seminiferous Tubules / Q000473:pathology*; D013091:Spermatogenesis / Q000502:physiology*
D001786:Blood Glucose; D018809:Proliferating Cell Nuclear Antigen