[Influence of selective versus nonselective inhibitors of nitric oxide synthases on synovial microcirculation of the knee joint of the mouse in vivo].
Nitric oxide production by the inducible NO-synthase in the synovium and chondrocytes is known to be enhanced during chronic joint inflammation and aseptic loosening of joint prostheses. Due to the distinct side effects of non-selective NO-inhibitors on the macro- and microhemodynamics, we investigated the in vivo changes after selective (N-iminoethyl-L-lysine (NIL)) versus non-selective NO-synthase inhibition (NG-nitro-L-arginine methyl ester (L-NAME)) in the synovium of the mouse knee joint. Our results show a significant decrease in the functional capillary density and an increase in the leukocyte accumulation after L-NAME injection. In contrast, NIL did not alter the microhemodynamics or the leukocyte-endothelial cell interaction in the synovium, indicating its potential use for therapeutic selective inhibition of iNOS in joint inflammation.
D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007719:Knee Joint / Q000098:blood supply*; D007958:Leukocyte Count; D008239:Lysine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation / Q000187:drug effects / Q000503:physiopathology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000502:physiology; D052247:Nitric Oxide Synthase Type II; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D013583:Synovial Membrane / Q000098:blood supply*; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology
D004791:Enzyme Inhibitors; C090663:N(6)-(1-iminoethyl)lysine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D008239:Lysine; D019331:NG-Nitroarginine Methyl Ester