Sequence and expression analysis of virB9 of the type IV secretion system of Ehrlichia canis strains in ticks, dogs, and cultured cells.
Ehrlichia canis virB9 was cloned and expressed. The sequences of virB9 from six geographic locations were identical. virB9 was transcribed by E. canis in dogs, ticks, and cell culture. Infected dogs had antibodies to recombinant VirB9, indicating that VirB9 was produced by E. canis in dogs and was antigenic.
D000595:Amino Acid Sequence; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D016873:Ehrlichiosis / Q000382:microbiology / Q000662:veterinary; D005260:Female; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000382:microbiology; D037521:Virulence Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D037521:Virulence Factors