Ribozymes as tools for suppression of G protein gamma subunits.
RNA suppression approaches provide a rapid survey of gene function. Of these approaches (i.e., antisense oligonucleotides, ribozymes, and RNA interference), ribozymes offer significant advantages by operating as stringent site-specific ribonucleases. The purpose of this chapter is to provide some guidelines and protocols for the use of ribozymes to identify the functions of closely related members of the G protein gamma subunit family. Improvements in their design and delivery will be discussed, including the use of "second generation" ribozymes targeted against multiple cleavage sites.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics* / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction
D044388:GTP-Binding Protein gamma Subunits; D016337:RNA, Catalytic