Molecular identification of Trichinella spiralis and Trichinella britovi by diagnostic multiprimer large mitochondrial rRNA amplification.
Trichinella parasites with different epidemiological features still occur in Europe and four species of genus Trichinella have been identified: T. spiralis, T. britovi, T. nativa and T. pseudospiralis. Until now, two of them, T. spiralis and T. britovi, have been identified in Poland. In our studies we selected sequence coding for large mitochondrial rRNA (mt LrDNA) as a genetic marker and developed a sensitive LrDNA multiprimer PCR assay allowing for rapid identification of T. spiralis and T. britovi, parasites present in wild and domestic animals in Poland.
D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D001483:Base Sequence; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D011044:Poland; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012313:RNA / Q000235:genetics; D018510:RNA, Helminth / Q000235:genetics; D000077278:RNA, Mitochondrial; D013552:Swine / Q000469:parasitology; D013553:Swine Diseases / Q000469:parasitology*; D017160:Trichinella spiralis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*
D018510:RNA, Helminth; D000077278:RNA, Mitochondrial; D012313:RNA