Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco.
cDNA encoding betaine aldehyde dehydrogenase (BADH) from the halophyte Suaeda liaotungensis has been cloned, sequenced and expressed in tobacco (Nictiana tabacum 89). The full-length cDNA is 1506 base pairs (bp) long and encodes a 502 amino-acid polypeptide. The cDNA fragment coding for the mature enzyme was cloned into vector pCAMBIA-1301 for expression in tobacco. Southern blotting analysis showed that that the Badh gene was integrated into the genome of tobacco. Tobacco expressing BADH survived on MS medium containing 200 mM NaCl, whereas the untransformed plants turned yellow after about 20 d and died.
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D050728:Betaine-Aldehyde Dehydrogenase; D019663:Chenopodiaceae / Q000201:enzymology* / Q000378:metabolism*; D002850:Chromatography, Gel; D003001:Cloning, Molecular / Q000379:methods*; D004351:Drug Resistance / Q000235:genetics; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000201:enzymology; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins; D017385:Sequence Homology; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
D011994:Recombinant Proteins; D012965:Sodium Chloride; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase