title: Non-cytocidal infection of keratinocytes by canine distemper virus in the so-called hard pad disease of canine distemper.
abstract: A late, but not uncommon sequel to canine distemper virus (CDV) infection of dogs is thickening of footpads and nasal planum, the so-called hard pad disease, originally described as vacuolar degeneration of epidermal keratinocytes with inclusion body formation and massive hyperkeratosis. However, in a recent study of footpads of naturally CDV-infected dogs only hyperkeratosis was observed without any of the other changes. Instead, acanthosis was frequently noticed. CDV nucleoprotein was present in the suprabasal keratinocytes and eccrine epithelial glands only. No CDV nucleoprotein was present in basal keratinocytes. This observation in combination with lack of obvious cytocidal changes strongly suggested the possibility of a restricted viral infection with presence of viral mRNA but without protein expression. Therefore, the presence of CDV nucleoprotein mRNA was investigated using in situ hybridization and compared to the localization of the nucleoprotein in footpads of clinically healthy and distemper dogs. Viral nucleoprotein and nucleoprotein mRNA in nearly all cases co-localized to the same compartments and basal keratinocytes did not contain nucleoprotein mRNA. These findings dispute the idea of a restricted viral infection of footpad keratinocytes in dogs with natural CDV infection. Instead, a migration of the virus to the epidermal surface along with the proliferating and differentiating epithelium is the most likely explanation for the lack of virus antigen in basal keratinocytes.
mesh_terms: D000818:Animals; D004216:Distemper / Q000150:complications / Q000473:pathology* / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000472:pathogenicity*; D004285:Dogs; D005534:Foot Diseases / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D015603:Keratinocytes / Q000473:pathology / Q000821:virology*; D012333:RNA, Messenger / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D012333:RNA, Messenger; D014764:Viral Proteins