Virus-induced gene silencing.
In the postgenomic era, large-scale functional genomic approaches are necessary for converting sequence information into functional information. A para-genetic approach, called virus-induced gene silencing (VIGS), offers a rapid means of gaining insight into gene function in plants. VIGS system could be used to suppress endogenous gene expression by infecting plants with a recombinant virus vector (VIGS vector) carrying host-derived sequence. Here, we describe the use of tobacco rattle virus (TRV)-based VIGS technique to study gene function in Nicotiana benthamiana and tomato.
D020868:Gene Silencing; D005821:Genetic Techniques; D005822:Genetic Vectors; D018551:Solanum lycopersicum / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000235:genetics*