title: Glycine mediated alterations in intracellular pH.
abstract: Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005849:Gerbillinae; D005998:Glycine / Q000494:pharmacology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects* / Q000502:physiology; D009847:Olivary Nucleus / Q000187:drug effects / Q000502:physiology
chemical_list: D005998:Glycine