title: Nutritional requirements for Hydrogenomonas eutropha.
abstract: Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammonium, nitrate, or urea, was used for growth; nitrite could not be utilized. Optimal growth occurred at pH 6.4 to 6.8 at 30 C. H. eutropha grew best in an atmosphere containing 15 to 25% oxygen and 10% carbon dioxide. Below these concentrations each of the gases was limiting. Growth was shown to be dependent on iron, and the rate of growth was a function of iron concentration and its state of oxidation.
mesh_terms: D002245:Carbon Dioxide; D042621:Comamonadaceae / Q000208:ethnology*; D003470:Culture Media; D007501:Iron; D009584:Nitrogen; D009751:Nutritional Requirements; D010084:Oxidation-Reduction; D010100:Oxygen
chemical_list: D003470:Culture Media; D002245:Carbon Dioxide; D007501:Iron; D009584:Nitrogen; D010100:Oxygen
keywords: CULTURE MEDIA; HYDROGENOMONAS/culture