Glycine mediated alterations in intracellular pH.
Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.
D000818:Animals; D000831:Animals, Newborn; D005849:Gerbillinae; D005998:Glycine / Q000494:pharmacology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects* / Q000502:physiology; D009847:Olivary Nucleus / Q000187:drug effects / Q000502:physiology
D005998:Glycine