[Cloning and sequencing of human vascular endothelial growth factor-C encoded cDNA].
OBJECTIVE
A functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in order to study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignancies in gene level.


METHODS
RT-PCR was employed to clone the human VECF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then it was subcloned into plasmid vector pCRII and sequenced.


RESULTS
A 1.1 kb human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1,611 bp according the sequence of Genbank Entry X94216).


CONCLUSIONS
An encoded fragment VEGF-C cDNA was successfully cloned from a lingual SCC and provided a necessary material for further study.
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA; D014062:Tongue Neoplasms / Q000235:genetics*; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
D018076:DNA, Complementary; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C