Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus.
Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.
D000818:Animals; D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D002448:Cell Adhesion; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009154:Mutation; D010641:Phenotype; D014774:Virulence / Q000235:genetics
D005656:Fungal Proteins