[Studies on the simvastatin effect on the artery of atherosclerotic rabbit using proteomics approaches].
OBJECTIVE
To evaluate the inhibitory effect of simvastatin via investigating the overall expression level of proteins in the artery of atherosclerotic rabbit.


METHODS
Experimental model was established by feeding the rabbits a high fat diet (cholesterol 0.5 g.kg-1.d-1, lard 0.5 mL.kg-1.d-1) for 8 weeks. Then simvastatin (5 mg.kg-1) were fed for 4 weeks to the rabbits. The overall protein levels were measured using two-dimensional gel electrophoresis and a PDQUEST data processing.


RESULTS
Twenty nine protein spots showed significant quantitative changes in comparison with the normal and the diseased rabbits. Furthermore, after the diseased rabbit having taken simvastatin, an obvious decay of symptom of fatty liver was observed, and the intensity of most spots has not been back-regulated.


CONCLUSIONS
Simvastatin facilitates the metabolism of fat in the blood, but the lesion of the internal wall of the atherosclerotic artery cannot be restored.
D000818:Animals; D001158:Arteries / Q000187:drug effects* / Q000473:pathology; D001161:Arteriosclerosis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004036:Diet, Atherogenic; D005234:Fatty Liver / Q000473:pathology; D000960:Hypolipidemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D019821:Simvastatin / Q000494:pharmacology* / Q000627:therapeutic use
D000960:Hypolipidemic Agents; D011506:Proteins; D019821:Simvastatin