Purification of G protein subunits from Sf9 insect cells using hexahistidine-tagged alpha and beta gamma subunits.
G protein-mediated pathways are the most fundamental mechanisms of cell signaling. In order to analyze these pathways, the availability of purified recombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D002845:Chromatography / Q000379:methods*; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000302:isolation & purification; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000302:isolation & purification; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics / Q000302:isolation & purification; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D006639:Histidine / Q000378:metabolism; D007313:Insecta; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*; D015398:Signal Transduction
D044385:GTP-Binding Protein alpha Subunits; D044387:GTP-Binding Protein beta Subunits; D044388:GTP-Binding Protein gamma Subunits; D011994:Recombinant Proteins; D006639:Histidine; D020962:Heterotrimeric GTP-Binding Proteins