Atrial cardiomyocyte-specific expression of Cre recombinase driven by an Nppa gene fragment.
To study the development of the atria, we produced a transgenic mouse line that expresses Cre under the regulatory control of a 7 kbp fragment of the Natriuretic peptide precursor type A gene (Nppa), from -3 kbp to +4 kbp relative to the transcription start site. Crossing this line with the R26R and Z/EG reporter lines revealed recombinase activity specifically in the cardiomyocytes of the atria and to a lesser extent the inflow tract from E10.5 onwards. At E14.5 recombination in the atria is almost complete. No recombination was observed outside the heart. These mice provide a tool to study gene function in the atria.
D000818:Animals; D009320:Atrial Natriuretic Factor; D002460:Cell Line; D017930:Genes, Reporter; D006325:Heart Atria / Q000166:cytology* / Q000196:embryology; D006579:Heterozygote; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D032383:Myocytes, Cardiac / Q000201:enzymology*; D045283:Natriuretic Agents / Q000737:chemistry; D020098:Natriuretic Peptide, C-Type / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D011995:Recombination, Genetic; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D045283:Natriuretic Agents; C431578:Nppa protein, mouse; D011498:Protein Precursors; D014764:Viral Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase