Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes.
Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark.
D001391:Azo Compounds / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D010777:Photochemistry; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D001391:Azo Compounds; D004247:DNA; C070852:RNA polymerase SP6; D012321:DNA-Directed RNA Polymerases; C009850:azobenzene