Synthesis, characterization, DNA-binding and cleavage studies of [Ru(bpy)2(actatp)]2+ and [Ru(phen)2(actatp)]2+ (actatp=acenaphthereno[1,2-b]-1,4,8,9-tetraazariphenylence).
New ligand acenaphthereno[1,2-b]-1,4,8,9-tetraazariphenylence (actatp) and its complexes [Ru(bpy)(2)(actatp)](ClO(4))(2).2H(2)O (1) (bpy=2,2'-bipyridine) and [Ru(phen)(2)(actatp)](ClO(4))(2).2H(2)O (2) (phen=1,10-phenanthroline) have been synthesized and characterized by UV-vis, 1H NMR, and mass spectra. The electrochemical behavior of the two complexes was studied by cyclic voltammetry. The interaction of the two complexes with calf thymus DNA has been investigated by spectrophotometric methods and viscosity measurements. The experimental results suggest that both complexes bind to DNA through an intercalative mode. The circular dichroism signals of the dialysates of the racemic complexes against calf thymus DNA are discussed. When irradiated at 302 nm, both complexes have also been found to promote the photocleavage of plasmid pBR 322 DNA.
D015082:2,2'-Dipyridyl / Q000737:chemistry; D000073:Acenaphthenes / Q000737:chemistry*; D001667:Binding, Competitive; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010083:Oxidants, Photochemical / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D010777:Photochemistry; D010957:Plasmids / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013816:Thermodynamics; D014783:Viscosity
C487124:(acenaphthereno(1,2-b)-1,4,8,9-tetraazariphenylence)bis(1,10-phenanthroline)ruthenium(II); C487123:(acenaphthereno(1,2-b)-1,4,8,9-tetraazariphenylence)bis(2,2'-bipyridine)ruthenium(II); D000073:Acenaphthenes; D007364:Intercalating Agents; D009942:Organometallic Compounds; D010083:Oxidants, Photochemical; D010618:Phenanthrolines; C415335:acenaphtheno(1,2-b)-1,4,8,9-tetraazatriphenylene; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; D004996:Ethidium; C025205:1,10-phenanthroline