Metabolite profiling as a functional genomics tool.
Plants accumulate a very large number of small molecules (phytochemicals) with important functions in the ecology of plants and in the protection against biotic and abiotic stress conditions. Little is known on how phytochemical biosynthetic pathways are regulated, which is a key step to successfully engineering plant metabolism. Plant natural products are usually not essential, and genetic analyses often fail to identify phenotypes associated with the absence of these compounds. We have investigated the use of metabolite profiling of plant cells in culture to establish the function of transcription factors suspected to control plant metabolic pathways.
D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling / Q000379:methods*; D018745:Genome, Plant; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D003313:Zea mays / Q000166:cytology / Q000235:genetics*
D018744:DNA, Plant