Production of a thermostable extracellular lipase by Kluyveromyces marxianus.
Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g tributyrin l(-1). Addition of surfactants (1 g l(-1)) did not improve production. The lipase had a high thermal stability in aqueous solution (73% residual activity after 9 d at 50 degrees C, 16 min half-life time at 100 degrees C). It was also stable at acidic pH and showed good tolerance to organic solvents (70% residual activity after 2 d in n-hexane of cyclohexane).
D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007716:Kluyveromyces / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D050356:Lipid Metabolism; D011489:Protein Denaturation; D013696:Temperature; D014280:Triglycerides / Q000378:metabolism*
D014280:Triglycerides; D008049:Lipase; C005830:tributyrin