[Oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation].
OBJECTIVE
To explore whether the oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation.


METHODS
Intracellular mean fluorescence intensity was measured by flow cytometry.


RESULTS
After treatment with FITC-labeled G3139 at the concentration of 0.60 mumol.L-1 for 4 h, the G3139 uptake into peripheral blood mononuclear cell and bone marrow mononuclear cell in hematological tumor patients was significantly higher than that in normal control. There was different uptake of G3139 among the malignant hematological tumor cell strains, and the uptake in cells derived from monocyte, B lymphocyte and myeloid cell was much higher than that in cells derived from T lymphocyte. After treatment with all-trans retinoic acid (ATRA), HL60 cell proliferation was markedly inhibited and the uptake of G3139 decreased significantly.


CONCLUSIONS
Hematological tumor cells were capable of taking up oligonucleotide, and the oligonucleotide uptake in hematological tumor cells is related to its cellular species and its activation.
D001692:Biological Transport; D002455:Cell Division / Q000502:physiology; D019254:Genes, bcl-2 / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000473:pathology; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D013873:Thionucleotides / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D014212:Tretinoin; C408162:oblimersen