Molecular cloning and characterization of a novel lectin gene from Lycoris radiata.
By the use of RACE-PCR with RNA extracted from Lycoris radiata young leaves, the full-length cDNA of L. radiata agglutinin (LRA) was cloned. The full-length cDNA of LRA was 669 bp and contained a 477 bp open reading frame encoding a 158 amino acid protein. Through comparative analysis of lra gene and its deduced amino acid sequence with those of other Amaryllidaceae species, it was found that lra encoded a precursor lectin with signal peptide. LRA was a mannose-binding lectin with three mannose-binding boxes like lectins from other Amaryllidaceae species. Southern blot analysis of genomic DNA revealed that lra belonged to a low copy gene family. Northern blot analysis demonstrated that lra constitutively expressed in various plant tissues including leaf, root, stem and inflorescence, and highly expressed in the inflorescence.
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017931:DNA Primers; D015870:Gene Expression; D031426:Lycoris / Q000235:genetics*; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D037601:Mannose-Binding Lectin