Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP.
USP7 or HAUSP is a ubiquitin-specific protease in human cells that regulates the turnover of p53 and is bound by at least two viral proteins, the ICP0 protein of herpes simplex type 1 and the EBNA1 protein of Epstein-Barr virus. We have overexpressed and purified USP7 and shown that the purified protein is monomeric and is active for cleaving both a linear ubiquitin substrate and conjugated ubiquitin on EBNA1. Using partial proteolysis of USP7 coupled with matrix-assisted laser desorption ionization time-of-flight mass spectrometry, we showed that USP7 comprises four structural domains; an N-terminal domain known to bind p53, a catalytic domain, and two C-terminal domains. By passing a mixture of USP7 domains over EBNA1 and ICP0 affinity columns, we showed that the N-terminal p53 binding domain was also responsible for the EBNA1 interaction, while the ICP0 binding domain mapped to a C-terminal domain between amino acids 599-801. Tryptophan fluorescence assays showed that an EBNA1 peptide mapping to residues 395-450 was sufficient to bind the USP7 N-terminal domain and did so with a dissociation constant of 0.9-2 microM, whereas p53 peptides spanning the USP7-binding region gave dissociation constants of 9-17 microM in the same assay. In keeping with these relative affinities, gel filtration analyses of the complexes showed that the EBNA1 peptide efficiently competed with the p53 peptide for USP7 binding, suggesting that EBNA1 could affect p53 function in vivo by competing for USP7.
D015153:Blotting, Western; D002384:Catalysis; D020134:Catalytic Domain; D002412:Cations; D002850:Chromatography, Gel; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017874:Immediate-Early Proteins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry; D011480:Protease Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012492:Salts / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry; D043222:Ubiquitin Thiolesterase; D044767:Ubiquitin-Protein Ligases; D000076084:Ubiquitin-Specific Peptidase 7
D002412:Cations; D018076:DNA, Complementary; D019309:Epstein-Barr Virus Nuclear Antigens; D017874:Immediate-Early Proteins; D010455:Peptides; D011480:Protease Inhibitors; D012492:Salts; D016159:Tumor Suppressor Protein p53; D025801:Ubiquitin; D014364:Tryptophan; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1; D010450:Endopeptidases; C464569:USP7 protein, human; D043222:Ubiquitin Thiolesterase; D000076084:Ubiquitin-Specific Peptidase 7; C417029:EBV-encoded nuclear antigen 1