title: High genetic diversity in the coat protein and 3  untranslated regions among geographical isolates of Cardamom mosaic virus from south India.
abstract: A survey was conducted to study the biological and genetic diversity of Cardamom mosaic virus (CdMV) that causes the most widespread disease in the cardamom growing area in the Western Ghats of south India. Six distinct subgroups were derived based on their symptomatology and host range from the sixty isolates collected. The serological variability between the virus isolates was analysed by ELISA and Western blotting. The 3  terminal region consisting of the coat protein (CP) coding sequence and 3  untranslated region (3 UTR) was cloned and sequenced from seven isolates. Sequence comparisons revealed considerable genetic diversity among the isolates in their CP and 3 UTR, making CdMV one of the highly variable members of Potyviridae. The possible occurrence of recombination between the isolates and the movement of the virus in the cardamom tract of south India are discussed.
mesh_terms: D020413:3' Untranslated Regions / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics*; D028523:Elettaria / Q000821:virology*; D014644:Genetic Variation; D007194:India; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010802:Phylogeny; D010942:Plant Viruses / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D020413:3' Untranslated Regions; D036022:Capsid Proteins