[The inhibit effect of berberine on human colon cell line cyclooxygenase-2].
OBJECTIVE
To investigate the effect of berberine on human colon cancer cell line HT-29 and the mechanisms of the effects.


METHODS
Add 0.1, 0.3, 3.0, 30.0 micro mol/L berberine into the HT-29 cell culture media. The related values were recorded everyday. NS-398 was used as a cyclooxygenase-2 (COX-2) enzyme activity inhibiting agent. The related values included cell growth, test of COX-2 mRNA using RT-PCR method, expression of COX-2 protein with immunocytochemical method, and concentration of prostaglandin E(2) with ELISA method.


RESULTS
When the concentration of berberine was higher than 0.3 micro mol/L, there was a significant dose-dependent effect. When the berberine concentration was higher than 0.3 micro mol/L, berberine could inhibit the COX-2 in mRNA and protein level. It could decrease the concentration of prostaglandin E(2) in the culture media.


CONCLUSIONS
Berberine can inhibit COX-2 in mRNA and protein level. It can also inhibit the activity of COX-2. This may be one of the mechanisms that berberine inhibit the growth and proliferation of HT-29 human colon cell line.
D001599:Berberine / Q000494:pharmacology*; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000032:analysis; D019073:HT29 Cells; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000235:genetics; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D001599:Berberine; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone