Liquid chromatographic method for analysis of saponins in Maesa balansae extract active against leishmaniasis.
A liquid chromatographic method was developed for the separation of six related triterpenoid saponins in Maesa balansae extracts with different purity, active against leishmaniasis. As stationary phase a Hypersil BDS C18 column (3 microm), 100 x 4.6 mm was used. The mobile phase was a mixture of methanol, acetonitrile, 5% (m/v) ammonium acetate, pH 6.5 and water. A linear gradient was developed for the analysis of crude extracts. An isocratic method was developed to analyze purified samples that mainly contained saponins 3 and 4, the most active saponins. The isocratic LC method was optimized and the robustness was evaluated with an experimental design. The method showed good selectivity, repeatability, linearity and sensitivity.
D002853:Chromatography, Liquid / Q000379:methods*; D007896:Leishmaniasis / Q000188:drug therapy*; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000494:pharmacology; D012680:Sensitivity and Specificity
D010936:Plant Extracts; D012503:Saponins