Suspension culture of anchorage-dependent animal cells using nanospheres of the biodegradable polymer, poly(lactic-co-glycolic acid).
A novel method was developed for suspension culture of anchorage-dependent animal cells using biodegradable polymer nanospheres. The addition of poly(lactic-co-glycolic acid) nanospheres (907 nm average diam.) to the culture of human embryonic kidney 293 cells in stirred suspension bioreactors promoted the aggregate formation and cell growth (4.4-fold versus 2.2-fold growth for 7 d), compared to culture without nanospheres. This method may be useful for the culture of various types of anchorage-dependent animal cells in large-scale suspension bioreactors.
D000818:Animals; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology / Q000502:physiology* / Q000648:ultrastructure; D009038:Motion; D043942:Nanotubes; D023822:Tissue Engineering / Q000379:methods*