Serum amyloid P component binding to Shiga toxin 2 requires both a subunit and B pentamer.
Solid-phase binding, competitive binding, and cytotoxicity neutralization assays indicate that the B pentamer and A subunit both contribute to human serum amyloid P (HuSAP) component binding to Stx2. A polyvalent globotriaosyl-ceramide receptor analog, Daisy, did not competitively inhibit HuSAP binding, implying that the two ligands bind to different Stx2 domains.
D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D009500:Neutralization Tests; D009844:Oligosaccharides / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D022622:Shiga Toxin 1 / Q000737:chemistry / Q000378:metabolism; D022641:Shiga Toxin 2 / Q000737:chemistry / Q000378:metabolism*
C472587:Daisy carbohydrate; D008024:Ligands; D009844:Oligosaccharides; D021122:Protein Subunits; D000683:Serum Amyloid P-Component; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2