Site-selective DNA hydrolysis by the combination of Ce(IV) and oligonucleotide bearing EDTA groups.
By using appropriately modified oligonucleotides, a gap-structure is formed in substrate DNA, and 2-6 ethylenediamine-N,N,N'-triacetate residues are placed near this gap. When these composites are treated with homogenous Ce(IV)/EDTA complex, the phosphodiester linkages in the gap-site are selectively hydrolyzed at much greater rates than achieved previously by using unmodified oligonucelotides.
D001483:Base Sequence; D002563:Cerium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D002563:Cerium; D004247:DNA; D004492:Edetic Acid