title: Carbazole/dioxin-degrading car gene cluster is located on the chromosome of Pseudomonas stutzeri strain OM1 in a form different from the simple transposition of Tn4676.
abstract: The carbazole-degrading (car) operon on the chromosome of Pseudomonas stutzeri strain OM1 showed > 99% identity to that in the 72.8 kb catabolic transposon, Tn4676, on plasmid pCAR1. Southern hybridization using probes prepared from the pCAR1 sequence and sequencing analyses showed that the OM1 chromosome contained the 55 kb DNA region, almost all of which was a part of Tn4676, flanked by two copies of novel insertion sequence, ISPst3, and included the car gene.
mesh_terms: D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004147:Dioxins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D044223:Pseudomonas stutzeri / Q000201:enzymology* / Q000235:genetics*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: D002227:Carbazoles; D004251:DNA Transposable Elements; D004147:Dioxins; C041514:carbazole; D010105:Oxygenases