[The dosage of SDS when extracting membranous labyrinth proteins].
OBJECTIVE
To determine the appropriate dosage of SDS when extracting membranous labyrinth proteins.


METHODS
The yield of proteins extracted with various amounts of SDS were determined by BCA method, and the components of the extracted proteins were analyzed by SDS-PAGE.


RESULTS
The amount of proteins extracted with 0.1% SDS is less than that with 0.23%, 1% or 2% SDS, meanwhile it shows less bands than the other and the corresponding bands are stained weaker. 0.23% SDS is less efficiently than 1% and 2% SDS in solubilizing proteins. There is no significant difference between 2% and 1% SDS in yield of proteins, while 2% SDS accompanies a higher ratio of detergent to protein.


CONCLUSIONS
1% SDS is solutions is relatively suitable for extracting membranous labyrinth proteins extensively while the ratio of detergent to protein is from 5 to 6.
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D007758:Ear, Inner / Q000737:chemistry* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006168:Guinea Pigs; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate
D001323:Autoantibodies; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate