Fluorescence resonance energy transfer reveals a binding site of a photosensitizer for photodynamic therapy.
Phthalocyanine (Pc) 4, like many photosensitizers for photodynamic therapy (PDT), localizes to intracellular membranes, especially mitochondria. Pc 4-PDT photodamages Bcl-2 and Bcl-xL, antiapoptotic proteins interacting with the permeability transition pore complex that forms at contact sites between the inner and outer mitochondrial membranes. These complexes and the inner membrane are unique in containing the phospholipid cardiolipin. Nonyl-acridine orange (NAO) is a specific probe of cardiolipin. Here we show evidence for fluorescence resonance energy transfer from NAO to Pc 4, defining a binding site for the photosensitizer. This observation establishes an innovative tool for exploring the localization of other photosensitizers and additional fluorescent, mitochondrion-localizing drugs having appropriate spectral properties.
D000165:Acridine Orange / Q000031:analogs & derivatives* / Q000737:chemistry; D001665:Binding Sites; D002308:Cardiolipins / Q000737:chemistry / Q000378:metabolism*; D004396:Coloring Agents / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D018613:Microscopy, Confocal; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013347:Subcellular Fractions / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002308:Cardiolipins; D004396:Coloring Agents; D007211:Indoles; D017319:Photosensitizing Agents; C107448:phthalocyanine Pc 4; C056258:N(10)-nonylacridine orange; D000165:Acridine Orange