Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis.
The solution structure of the N-terminal region (151 amino acids) of a copper ATPase, CopA, from Bacillus subtilis, is reported here. It consists of two domains, CopAa and CopAb, linked by two amino acids. It is found that the two domains, which had already been separately characterized, interact one to the other through a hydrogen bond network and a few hydrophobic interactions, forming a single rigid body. The two metal binding sites are far from one another, and the short link between the domains prevents them from interacting. This and the surface electrostatic potential suggest that each domain receives copper from the copper chaperone, CopZ, independently and transfers it to the membrane binding site of CopA. The affinity constants of silver(I) and copper(I) are similar for the two sites as monitored by NMR. Because the present construct "domain-short link-domain" is shared also by the last two domains of the eukaryotic copper ATPases and several residues at the interface between the two domains are conserved, the conclusions of the present study have general validity for the understanding of the function of copper ATPases.
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D020816:Amino Acid Motifs; D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D027682:Cation Transport Proteins / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D003300:Copper / Q000737:chemistry; D000073840:Copper-Transporting ATPases; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D015534:Trans-Activators / Q000378:metabolism
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; C092379:CopZ protein, Enterococcus hirae; D018832:Molecular Chaperones; D015534:Trans-Activators; D003300:Copper; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases