An efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure D-tert-leucine.
[reaction: see text] d-tert-Leucine was prepared with an enantiomeric excess of >99% by an enzyme-catalyzed oxidative resolution of the racemic mixture of dl-tert-leucine with use of leucine dehydrogenase. The l-amino acid was oxidized completely due to coupling of the primary reaction with a highly efficient irreversible NAD(+)-regenerating step by NADH oxidase.
D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D001409:Bacillus cereus / Q000201:enzymology; D002384:Catalysis; D007700:Kinetics; D007930:Leucine / Q000737:chemistry / Q000031:analogs & derivatives; D050803:Leucine Dehydrogenase; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D013237:Stereoisomerism; D014633:Valine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D009097:Multienzyme Complexes; D009243:NAD; C089871:2-amino-3,3-dimethylbutanoic acid; D000594:Amino Acid Oxidoreductases; D050803:Leucine Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D007930:Leucine; D014633:Valine