title: A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 strong gyrase sites: the role of DNA sequence in modulating gyrase supercoiling and biological activity.
abstract: Replication of bacteriophage Mu DNA, a process requiring efficient synapsis of the prophage ends, takes place within the confines of the Escherichia coli nucleoid. Critical to ensuring rapid synapsis is the function of the SGS, a strong gyrase site, located at the centre of the Mu genome. Replacement of the SGS by the strong gyrase sites from pSC101 or pBR322 fails to support efficient prophage replication. To probe the unique SGS properties we undertook a biochemical analysis of the interaction of DNA gyrase with the Mu SGS, pSC101 and pBR322 sites. In binding and cleavage assays the order of efficacy was pSC101 > Mu SGS >> pBR322. However, in supercoiling assays the Mu SGS (cloned into pUC19) exhibited a strong enhancement of gyrase-catalysed supercoiling over pUC19 alone; the pSC101 site showed none and the pBR322 site gave a moderate improvement. Most striking was the Mu SGS-dependent increase in processivity of the gyrase reaction. This highly processive supercoiling coupled with efficient binding may account for the unique biological properties of the SGS. The results emphasize the importance of the DNA substrate as an active component in modulating the gyrase supercoiling reaction, and in determining the biological roles of specialized gyrase sites.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D010583:Bacteriophage mu / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D027081:DNA Gyrase / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics; D004278:DNA, Superhelical / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D039002:Prophages / Q000235:genetics / Q000254:growth & development; D011485:Protein Binding; D015363:Quinolones / Q000378:metabolism; D011815:R Factors / Q000378:metabolism*; D011995:Recombination, Genetic; D014779:Virus Replication / Q000235:genetics
chemical_list: D004278:DNA, Superhelical; D004279:DNA, Viral; D015363:Quinolones; D000255:Adenosine Triphosphate; D027081:DNA Gyrase; D002118:Calcium