Characterization and analysis of osteopontin-immobilized poly(2-hydroxyethyl methacrylate) surfaces.
A wide array of technologies exist for the characterization and quantification of molecules present at surfaces. We have used several of these experimental and instrumental techniques for the analysis of a novel biomaterial surface. Osteopontin, an extracellular matrix molecule involved in wound-healing processes, has been chosen as a relevant model protein to immobilize onto poly(2-hydroxyethyl methacrylate) [poly(HEMA)]. Electron spectroscopy for chemical analysis and time-of-flight secondary ion mass spectrometry were used to verify the surface chemistry and the presence of protein. Iodination of osteopontin yielded quantitative data supportive of dose-dependent immobilization. Enzyme-linked immunosorbent assay was also used to investigate the presence of osteopontin on poly(HEMA). Finally, the cell adhesive properties of immobilized osteopontin were confirmed by using a bovine aortic endothelial cell adhesion assay. The use of multiple tools to characterize the many facets of a biomaterial surface will undoubtedly improve our understanding of the surface and facilitate the amelioration of in vivo performance.
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018076:DNA, Complementary; D042783:Endothelial Cells / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007457:Iodine Radioisotopes; D008239:Lysine / Q000378:metabolism; D053495:Osteopontin; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012795:Sialoglycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018629:Spectrometry, Mass, Secondary Ion
D001672:Biocompatible Materials; D018076:DNA, Complementary; D007457:Iodine Radioisotopes; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D011102:Polyhydroxyethyl Methacrylate; D008239:Lysine