title: An alternative protocol for the preparation of the polytene chromosomes of larval Simulium damnosum s.l. (Diptera: Simuliidae).
mesh_terms: D000818:Animals; D002875:Chromosomes; D000431:Ethanol; D005404:Fixatives; D007814:Larva; D012469:Salivary Glands / Q000648:ultrastructure; D012843:Simuliidae / Q000145:classification / Q000235:genetics*; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D005404:Fixatives; D000431:Ethanol