A high-throughput capillary assay for bacterial chemotaxis.
We present a high-throughput capillary assay in order to characterize the chemotactic response of the E. coli bacterium. We measure the number of organisms attracted into an array of 96 capillary tubes containing the attractant L-aspartate. The effect of bacterial concentration on the chemotactic response is reported. Such high-throughput assay can be used to characterize bacterial chemotaxis function of a wide range of biochemical parameters.
D002633:Chemotaxis; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000502:physiology*