Changes in some characteristics between the wild and Al-tolerant coffee (Coffea arabica L.) cell line.
An aluminium (Al)-tolerant cell line (LAMt) of coffee (Coffea arabica L.) was obtained from a cell suspension culture and biochemically and molecularly characterized in an MS medium at half ionic strength and low pH. LAMt grew 30% more than the control line (susceptible to Al) in the presence of different concentrations of Al, showed a lower free Al concentration in the medium and had higher phospholipase C specific activity (80%). Membrane integrity of the LAMt was 50% greater than the control line when both were incubated in the presence of different Al concentrations (measured by Evans Blue uptake). Finally, the use of microsatellite primers revealed no difference in the DNA pattern of both cell lines.
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D000483:Alleles; D000535:Aluminum / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003069:Coffee / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D018895:Microsatellite Repeats / Q000235:genetics; D009994:Osmolar Concentration; D013054:Spectrophotometry, Atomic; D010738:Type C Phospholipases / Q000378:metabolism
D003069:Coffee; D017931:DNA Primers; D000535:Aluminum; D010738:Type C Phospholipases