title: Effects of moisture content in cigar tobacco on nicotine extraction. Similarity between soxhlet and focused open-vessel microwave-assisted techniques.
abstract: The effects of tobacco moisture on nicotine yield were investigated in this study. Soxhlet and microwave-assisted techniques were used to extract nicotine from cigar fillers of varying moisture contents (5-20%), using a polar (methanol) and a non-polar (isooctane) solvent. The extracts were analyzed by a gas chromatograph equipped with a flame-ionization detector. For both extraction techniques, higher nicotine yields were consistently obtained with methanol than with isooctane from the same samples. Solubility of nicotine salts in methanol but not in isooctane is the major cause of this observation. Moreover, pronounced effects of the tobacco moisture content on extraction efficiency were observed with isooctane but not with methanol. For microwave assisted extraction (MAE) with isooctane, nicotine yield increased from 3 to 70% as the moisture level in tobacco was raised from 3 to 13%, and leveled off thereafter. Similar observations were made with Soxhlet extraction. While MAE results were rationalized by the known cell-rupture process, a mechanism based on the interaction between the solvents and the structural components of the plant cells has been proposed to account for the observations made with Soxhlet extraction.
mesh_terms: D002849:Chromatography, Gas; D008872:Microwaves; D009538:Nicotine / Q000302:isolation & purification*; D014026:Nicotiana / Q000737:chemistry*; D014867:Water / Q000032:analysis*
chemical_list: D014867:Water; D009538:Nicotine