TEGDMA causes apoptosis in primary human gingival fibroblasts.
Previous in vivo studies have revealed that resins may generate a persistent inflammation of oral tissues and cell death as well. Apoptosis is an important regulated process that results in rapid cell death. This study tested the hypothesis that the comonomer triethyleneglycol-dimethacrylate (TEGDMA) causes apoptosis. The effects of TEGDMA on proliferation and apoptosis in primary oral fibroblasts were analyzed by light microscopy and flow cytometry (FACS; Annexin V-assay). TEGDMA at 5 and 7.5 mM inhibited proliferation after 24 hrs. No increased frequency of apoptosis or necrosis was observed with 1 mM or 2.5 mM TEGDMA after 24 hrs. Apoptosis and Annexin V-positive cells were observed with 5 mM and 7.5 mM TEGDMA by light microscopy after 24 hrs. A dramatic increase in apoptotic cells was detected by FACS after 24 hrs with 7.5 mM TEGDMA. Thus, TEGDMA was cytotoxic and "apoptotic" in a dose- and time-dependent manner.
D000704:Analysis of Variance; D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects*; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D003188:Composite Resins / Q000633:toxicity*; D003764:Dental Materials / Q000633:toxicity*; D004791:Enzyme Inhibitors; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009336:Necrosis; D011092:Polyethylene Glycols / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000633:toxicity*; D013997:Time Factors
D017304:Annexin A5; D003188:Composite Resins; D003764:Dental Materials; D004791:Enzyme Inhibitors; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols