Nardosinone enhances nerve growth factor-induced neurite outgrowth in a mitogen-activated protein kinase- and protein kinase C-dependent manner in PC12D cells.
The mechanism to enhance nerve growth factor (NGF, 2 ng/ml)-induced neurite outgrowth from PC12D cells by nardosinone isolated from Nardostachys chinensis was examined. It was shown that the potentiation of the NGF-induced neurite outgrowth by nardosinone was mitogen-activated protein (MAP) kinase-dependent, but was not accompanied by stimulation of NGF-induced increase in MAP kinase phosphorylation. Furthermore, this augmentation of NGF-induced neurite outgrowth was abolished by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that the enhancement of NGF-induced neurite outgrowth from PC12D cells by nardosinone involves activation of a down-stream step of the MAP kinase-dependent cascade of NGF coupled with PKC.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008855:Microscopy, Electron, Scanning; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D032404:Nardostachys / Q000737:chemistry; D020932:Nerve Growth Factor / Q000494:pharmacology* / Q000648:ultrastructure; D016501:Neurites / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D016716:PC12 Cells; D000081223:Polycyclic Sesquiterpenes; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*
D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C011513:nardosinone; D020932:Nerve Growth Factor; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases