Rejection and indirect revascularization of glycerin-preserved tracheal implant.
The objective of the following study was to evaluate antigenicity, malacia and revascularization in glycerin-preserved canine tracheal allografts. Trachea with six cartilage rings (2.4 to 3.1 cm) were distributed in three study groups: autograft (21), allograft (18) and glycerin-preserved (22). We implanted two segments from different groups in the greater omentum of dogs. After 28 days, latex was injected in the canine aorta before the segments were harvested. We evaluated number of sectors with functional vessels, number of vessels dyed in the submucosa, acute arteritis score, incidence of acute rejection, cartilage lesion score, and malacia. The autograft group had a larger number of dyed vessels than the glycerin-preserved group. The autograft group also had a higher average number of quadrants with functional vessels than the allograft group and the glycerin-preserved group. The allograft group had a higher mean score for acute arteritis than the autograft group and more acute rejection than the glycerin-preserved group. The cartilage lesion score did not show any significant difference between groups. Malacia was not observed in any tracheal segment. Overall, the glycerin-preserved tracheal implant had low antigenicity and good rigidity, but showed incomplete revascularization.
D000818:Animals; D003451:Cryoprotective Agents / Q000627:therapeutic use; D004285:Dogs; D005990:Glycerol / Q000627:therapeutic use; D006084:Graft Rejection / Q000276:immunology*; D018919:Neovascularization, Physiologic / Q000276:immunology*; D016377:Organ Transplantation / Q000379:methods / Q000502:physiology*; D014132:Trachea / Q000098:blood supply / Q000637:transplantation*; D014181:Transplantation Immunology / Q000276:immunology
D003451:Cryoprotective Agents; D005990:Glycerol