Chondroitin O-methyl ester: an unusual substrate for chondroitin AC lyase.
Chondroitin O-methyl ester was depolymerized by chondroitin AC lyase (EC 4.2.2.5) from Flavobacterium heparinum. The major product isolated from the depolymerization reaction was found to be methyl alpha-L-threo-hex-4-enopyranosyluronate-(1-->4)-2-acetamido-2-deoxy-alpha,beta-D-galactopyranoside.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D002807:Chondroitin / Q000031:analogs & derivatives; D002808:Chondroitin Lyases / Q000378:metabolism*; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D005417:Flavobacterium / Q000201:enzymology; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004187:Disaccharides; C487421:chondroitin O-methyl ester; D002807:Chondroitin; D002808:Chondroitin Lyases