title: Bicuculline induces synapse formation on primary cultured accessory olfactory bulb neurons.
abstract: To investigate the roles of the GABAergic inhibitory system of accessory olfactory bulb (AOB) in pheromonal memory formation, we have developed a primary culture system of AOB neurons, which had numerous excitatory and inhibitory synapses. Using this culture system of AOB neurons, we examined the correlation in rats between neuronal excitation and synaptic morphology by bicuculline-induced disinhibition of cultured AOB neurons. The exposure to bicuculline induced long-lasting oscillatory changes in the intracellular calcium level ([Ca2+]in) of cultured non-GABAergic multipolar neurons, which were identified as mitral/tufted cells (MT cells). These MT cells exhibited the appearance of dendritic filopodia structures after a 10-min treatment with bicuculline. By labelling presynaptic terminals with FM4-64, the appearance of new presynaptic terminals was clearly observed on newly formed filopodia after 120 min treatment with bicuculline. These results suggest that bicuculline-induced [Ca2+]in oscillation of MT cells induces the growth of filopodia and subsequently the formation of new presynaptic terminals. Furthermore, tetrodotoxin or the deprivation of extracellular calcium blocked bicuculline-induced synapse formation. The present results indicate that the long-lasting [Ca2+]in oscillation caused by bicuculline-induced disinhibition of cultured MT cells is significantly implicated in the mechanism underlying synapse formation on cultured AOB neurons. Our established culture system of AOB neurons will aid in clarifying the mechanism of synapse formation between AOB neurons and the molecular mechanism of pheromonal memory formation.
mesh_terms: D000199:Actins / Q000378:metabolism; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002478:Cells, Cultured; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D018756:GABA Antagonists / Q000494:pharmacology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry / Q000379:methods; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D011554:Pseudopodia / Q000187:drug effects / Q000378:metabolism; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013569:Synapses / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D016704:Synapsins / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
chemical_list: D000199:Actins; D000779:Anesthetics, Local; C092350:FM 4-64; D018756:GABA Antagonists; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011963:Receptors, GABA-A; D016194:Receptors, N-Methyl-D-Aspartate; D016704:Synapsins; D049452:Green Fluorescent Proteins; D013779:Tetrodotoxin; D011189:Potassium Chloride; D005968:Glutamate Decarboxylase; D002118:Calcium; D016257:Fura-2; D001640:Bicuculline