Stretching of single DNA molecules by LB technique for restriction site mapping.
Stretched single DNA molecules were prepared on substrates by Langmuir-Blodgett (LB) method for the restriction site mapping. Stretched single Lambda DNA and EcoRI bound to the DNA were observed by scanning near-field optical microscope (SNOM). As a result, the specific binding sites of EcoRI on the Lambda DNA molecules were determined.
D004247:DNA / Q000737:chemistry*; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry*; D005453:Fluorescence; D015183:Restriction Mapping
D004247:DNA; D015246:Deoxyribonuclease EcoRI