A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach.
A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allele- specific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.
D019921:Biotinylation; D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000032:analysis; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant; D005456:Fluorescent Dyes; D005819:Genetic Markers; D015345:Oligonucleotide Probes