Investigation of physical and physiological properties of 4'-thioribonucleotide (4'-thioRNA).
An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.
D001483:Base Sequence; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012265:Ribonucleotides / Q000737:chemistry*
D012265:Ribonucleotides