title: Phosphoproteins in Escherichia coli.
abstract: Rafter, Gale W. (The Johns Hopkins School of Medicine and School of Hygiene and Public Health, Baltimore, Md.) and William C. Lane. Phosphoproteins in Escherichia coli. J. Bacteriol. 83:1077-1083. 1962.-The identification and metabolism of phosphoprotein were investigated in Escherichia coli. Hydrolysis of bacterial protein fractions with barium hydroxide or with phosphoprotein phosphatase released acid-soluble phosphorus. Chromatography of acid-hydrolyzed and incubated fractions also indicated the presence of phospho-amino acids. Turnover of phosphate in protein of growing cells was not observed, but incorporation of phosphate into protein of nongrowing cells was found. The protein-phosphate content decreased as organisms passed from the growing to the nongrowing state. The phosphoprotein composition, as revealed by paper electrophoresis, was heterogeneous. No protein phosphokinase or protein-phosphate phosphatase was detected in cell-free extracts, but an active principle which catalyzed the formation of acid-soluble phosphate from bacterial protein fractions was found.
mesh_terms: D001426:Bacterial Proteins; D004926:Escherichia coli / Q000378:metabolism*; D010750:Phosphoproteins; D011506:Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010750:Phosphoproteins; D011506:Proteins
keywords: ESCHERICHIA COLI/metabolism; PROTEINS/metabolism