The augertoxins: biochemical characterization of venom components from the toxoglossate gastropod Terebra subulata.
We describe the purification and biochemical characterization of three components from the venom of the toxoglossate gastropod Terebra subulata. The three polypeptide venom components, augertoxins s6a, s7a and s11a, are 40-41AA in length with 3-4 disulfide linkages. The arrangement of Cys residues is reminiscent of certain conopeptide superfamilies, but molecular cloning failed to show the highly conserved sequence features diagnostic of the conopeptide gene superfamily with a similar arrangement of Cys residues. One of the purified peptides, s7a, elicited an uncoordinated twisting syndrome when injected into the nematode Caenorhabditis elegans, but had no effect on mice. T. subulata belongs to the family Terebridae, one of four major groups of toxoglossate gastropods in the superfamily Conacea. The results reveal that some features of the augertoxins and conotoxins are generally similar, such as the organization of prepropeptide precursors and their proteolytic processing into mature toxins; however, Terebra may have evolved generally larger venom components that are less highly post-translationally modified. The results suggest that Conus peptide gene superfamilies probably do not extend to the Terebridae, suggesting that distinctive venom gene superfamilies may be expressed in each major division of Conacean gastropods.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020916:Conotoxins / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D011487:Protein Conformation; D012313:RNA / Q000032:analysis; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D012908:Snails / Q000737:chemistry*
D020916:Conotoxins; D018076:DNA, Complementary; D008978:Mollusk Venoms; D012313:RNA