title: Subcellular localization of adenosine A(1) receptors in nerve terminals and synapses of the rat hippocampus.
abstract: Adenosine is a neuromodulator in the CNS that mainly acts through pre- and postsynaptic A(1) receptors to inhibit the release of excitatory neurotransmitters and NMDA receptor function. This might result from a highly localized distribution of A(1) receptors in the active zone and postsynaptic density of CNS synapses that we now investigated in the rat hippocampus. The binding density of the selective A(1) receptor antagonist, [3H]1,3-dipropyl-8-cyclopentylxanthine ([3H]DPCPX), was enriched in membranes from Percoll-purified nerve terminals (B(max)=1839+/-52 fM/mg protein) compared to total membranes from the hippocampus (B(max)=984+/-31 fM/mg protein), the same occurring with A(1) receptor immunoreactivity. [3H]DPCPX binding occurred mainly to the plasma membrane rather than to intracellular sites, since the binding of the membrane permeable A(1) receptor ligand [3H]DPCPX to intact hippocampal nerve terminals (B(max)=1901+/-192 fM/mg protein) was markedly reduced (B(max)=321+/-30 fM/mg protein) by the membrane impermeable adenosine receptor antagonist, 8-sulfophenyltheophilline (25 microM). Further subcellular fractionation of hippocampal nerve terminals revealed that A(1) receptor immunoreactivity was strategically located in the active zone of presynaptic nerve terminals, as expected to understand the efficiency of A(1) receptors to depress neurotransmitter release. A(1) Receptors were also present in nerve terminals outside the active zone in accordance with the existence of a presynaptic A(1) receptor reserve. Finally, A(1) receptor immunoreactivity was evident in the postsynaptic density together with NMDA receptor subunits 1, 2A and 2B and with N-and P/Q-type calcium channel immunoreactivity, emphasizing the importance of A(1) receptors in the control of dendritic integration.
mesh_terms: D058916:Adenosine A1 Receptor Antagonists; D000818:Animals; D015153:Blotting, Western; D015220:Calcium Channels / Q000378:metabolism; D020864:Calcium Channels, N-Type / Q000378:metabolism; D006624:Hippocampus / Q000737:chemistry*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D009474:Neurons / Q000737:chemistry*; D017729:Presynaptic Terminals / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D043682:Receptor, Adenosine A1 / Q000032:analysis*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D013569:Synapses / Q000737:chemistry*; D014316:Tritium; D014970:Xanthines / Q000378:metabolism
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D015220:Calcium Channels; D020864:Calcium Channels, N-Type; D053496:Inositol 1,4,5-Trisphosphate Receptors; D043682:Receptor, Adenosine A1; D018160:Receptors, Cytoplasmic and Nuclear; D014970:Xanthines; D014316:Tritium; C051360:1,3-dipropyl-8-cyclopentylxanthine