title: Proteomic sensitivity to dietary manipulations in rainbow trout.
abstract: Changes in dietary protein sources due to substitution of fish meal by other protein sources can have metabolic consequences in farmed fish. A proteomics approach was used to study the protein profiles of livers of rainbow trout that have been fed two diets containing different proportions of plant ingredients. Both diets control (C) and soy (S) contained fish meal and plant ingredients and synthetic amino acids, but diet S had a greater proportion of soybean meal. A feeding trial was performed for 12 weeks at the end of which, growth and protein metabolism parameters were measured. Protein growth rates were not different in fish fed different diets; however, protein consumption and protein synthesis rates were higher in the fish fed the diet S. Fish fed diet S had lower efficiency of retention of synthesised protein. Ammonia excretion was increased as well as the activities of hepatic glutamate dehydrogenase and aspartate amino transferase (ASAT). No differences were found in free amino acid pools in either liver or muscle between diets. Protein extraction followed by high-resolution two-dimensional electrophoresis, coupled with gel image analysis, allowed identification and expression of hundreds of protein. Individual proteins of interest were then subjected to further analysis leading to protein identification by trypsin digest fingerprinting. During this study, approximately 800 liver proteins were analysed for expression pattern, of which 33 were found to be differentially expressed between diets C and S. Seventeen proteins were positively identified after database searching. Proteins were identified from diverse metabolic pathways, demonstrating the complex nature of gene expression responses to dietary manipulation revealed by proteomic characterisation.
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D017756:Aquaculture; D004044:Dietary Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010449:Peptide Mapping; D010940:Plant Proteins / Q000008:administration & dosage / Q000378:metabolism*; D020543:Proteome
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D010940:Plant Proteins; D020543:Proteome; D000641:Ammonia