Mass spectrometric detection and quantification of nodularin-R in flounder livers.
Livers of flounders caught during August 1995 from the western Gulf of Finland and the Archipelago Sea were analyzed for nodularin-R (NODLN) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and liquid chromatography-electrospray ionization-mass spectrometry (LC-MS). Results showed that NODLN was detected in samples by both MS techniques. NODLN content in samples varied between 0.082 and 0.637 microg g(-1) wet weight by LC-MS. Biotransformation products such as glutathione adduct were not found in the samples. The results showed that intact NODLN can be found in tissues after storage at -70 degrees C for several years.
D000818:Animals; D001711:Biotransformation; D003603:Cytotoxins / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*; D005432:Flounder; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry; D010456:Peptides, Cyclic / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*
D003603:Cytotoxins; D010456:Peptides, Cyclic; C063998:nodularin