title: Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus.
abstract: Physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated Malpighian tubules (MTs) of Rhodnius prolixus. Here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. The glutamine-dependent increase in MT fluid secretion rate was blocked by two chemically unrelated inhibitors of the stress activated protein kinase (SAPK) pathway, SP600125 and dicumarol. Inhibitors of phosphatidyl inositol-3 kinase, p38 mitogen activated protein kinase (MAPK), extracellular-signal regulated kinases and MAPK kinase did not block glutamine's effects on fluid secretion rate when applied at commonly used concentrations. Inhibitors of protein kinase A or C reduced fluid secretion rates of serotonin-stimulated MTs, but did not block the response to glutamine. The glutamine-dependent increase in fluid secretion was also insensitive to cytoskeletal disrupting agents and protein synthesis inhibitors. Results of this study are the first to suggest a role for the SAPK pathway in the control of fluid secretion rates by insect MTs.
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D001728:Dicumarol / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005973:Glutamine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008317:Malpighian Tubules / Q000378:metabolism*; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012239:Rhodnius / Q000502:physiology*; D012636:Secretory Rate / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000378:metabolism
chemical_list: D000873:Anthracenes; D004791:Enzyme Inhibitors; D011500:Protein Synthesis Inhibitors; D005973:Glutamine; C432165:pyrazolanthrone; D012701:Serotonin; D001728:Dicumarol; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases