Crystallization and initial X-ray diffraction studies of higher plant photosystem I.
Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 A resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P2(1), with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 A, beta = 90.484 degrees. Data sets from three crystals have been collected and were merged into a 4.4 A resolution data set, taking advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit.
D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D018532:Pisum sativum / Q000737:chemistry; D045331:Photosystem I Protein Complex / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011487:Protein Conformation; D017356:Synchrotrons
D045331:Photosystem I Protein Complex; D010940:Plant Proteins