title: Gated importation of prothymocytes by adult mouse thymus is coordinated with their periodic mobilization from bone marrow.
abstract: The wavelike pattern of fetal T cell neogenesis is largely determined by the intermittent generation and exportation of waves of prothymocytes by the hemopoietic tissues in coordination with their gated importation by the thymus. Having previously shown that the importation of prothymocytes by the adult mouse thymus is also gated and that thymocytopoiesis proceeds in discrete (albeit overlapping) waves, we now demonstrate that prothymocytes are periodically exported in saturating numbers from the adult mouse bone marrow. Experiments in normal, radioablated, and parabiotic mice document the cyclical accumulation (3-5 wk) of prothymocytes in both the steady state and regenerating bone marrow, followed by their release into the blood approximately 1 wk before intrathymic gate opening. The results also show that circulating donor-origin thymocyte precursors can transiently ( approximately 1 wk) establish high level chimerism in the bone marrow after the mobilization of endogenous prothymocytes, presumably by occupying vacated microenvironmental niches. Hence, by analogy with the fetal state, we posit the existence of a feedback loop whereby diffusible chemokines of thymic origin regulate the production and/or release of bone marrow prothymocytes during each period of thymic receptivity. Because each resulting wave of thymocytopoiesis is accompanied by a wave of intrathymic dendritic cell formation, these coordinated events may help to optimize thymocyte selection as well as production.
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000473:pathology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D005246:Feedback; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000473:pathology; D038041:Lymphopoiesis / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D010227:Parabiosis / Q000379:methods; D011828:Radiation Chimera / Q000276:immunology; D012038:Regeneration / Q000235:genetics / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology* / Q000473:pathology; D013997:Time Factors