Direct immunoassay of estrone by capillary electrophoresis with laser-induced fluorescence detection.
An immunoassay for estrone (E(1)) in women's serum, based on the competitive reaction between fluorescein-labeled complete antigen and E(1) with limited amount of anti-estrone monoclonal antibody is described. A thermally reversible hydrogel, poly-N-isopropylacrylamide (pNIPA), was added to the buffer to improve the reproducibility. With a laser-induced fluorescence (LIF) detector, the capillary electrophoretic immunoassay (CEIA) can be applied to determine E(1) at a concentration lower than 19.6 pg/mL. The E(1) levels in ten normal women's serum were measured at the range of 118.6-222.0 pg/mL.
D000180:Acrylic Resins / Q000737:chemistry; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D019075:Electrophoresis, Capillary / Q000379:methods*; D004970:Estrone / Q000097:blood* / Q000276:immunology; D005260:Female; D005453:Fluorescence; D006801:Humans; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D013997:Time Factors
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D020100:Hydrogels; C052970:poly-N-isopropylacrylamide; D004970:Estrone