Isolation of cucurmoschin, a novel antifungal peptide abundant in arginine, glutamate and glycine residues from black pumpkin seeds.
A novel antifungal peptide, with a molecular mass of 8 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in gel filtration on Superdex 75 and designated cucurmoschin, was isolated from the seeds of the black pumpkin. The peptide was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. Cucurmoschin inhibited mycelial growth in the fungi Botrytis cinerea, Fusarium oxysporum and Mycosphaerella oxysporum. It inhibited translation in a cell-free rabbit reticulocyte lysate system with an IC50 of 1.2 microM. The N-terminal sequence of cucurmoschin was rich in arginine, glutamate and glycine residues.
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D001120:Arginine / Q000032:analysis; D020171:Botrytis / Q000187:drug effects; D002474:Cell-Free System; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D028464:Cucurbita / Q000737:chemistry*; D023082:Defensins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D018698:Glutamic Acid / Q000032:analysis; D005998:Glycine / Q000032:analysis; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry
D000935:Antifungal Agents; D023082:Defensins; D010940:Plant Proteins; D014227:Triazines; C487127:cucurmoschin protein, Cucurbita moschata; D018698:Glutamic Acid; C012842:Cibacron Blue F 3GA; D001120:Arginine; D005998:Glycine