Gene and enhancer traps for gene discovery.
Gene traps and enhancer traps provide a valuable tool for gene discovery. With this system, genes can be identified based solely on the expression pattern of an inserted reporter gene. The use of a reporter gene, such as beta-glucuoronidase (GUS), provides a very sensitive assay for the identification of tissue- and cell-type specific expression patterns. In this chapter, protocols for examining and documenting GUS reporter gene activity in individual lines are described. Methods for the amplification of sequences flanking transposant insertions and subsequent molecular and genetic characterization of individual insertions are provided.
D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D005821:Genetic Techniques; D005966:Glucuronidase / Q000235:genetics; D007202:Indicators and Reagents; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004251:DNA Transposable Elements; D018744:DNA, Plant; D007202:Indicators and Reagents; D005966:Glucuronidase