[Study on the active spirostanol saponins of Gualou xiebai baijiutang].
OBJECTIVE
To study the basis of actions of Gualou xiebai baijiutang.


METHODS
Guided by bioactivities, chemical and chromatographic ways were applied to isolate and purify the prescription. The chemical structures were identified by chemical and spectral ways. The activities on cardiovascular system of the pure compounds were measured.


RESULTS
Seven steroidal compounds were isolated and identified from the active parts.


CONCLUSIONS
Compounds 5 and 6 showed good anti-platelet aggregation activities induced by ADP and PAF. The IC50 of compound 5 and 6 induced by 2 mumol.L-1 ADP were 0.082 and 0.078 mmol.L-1, and the IC50 induced by 0.5 mumol.L-1 PAF were 0.182 and 0.151 mmol.L-1, respectively.
D000490:Allium / Q000737:chemistry*; D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D000431:Ethanol; D005638:Fruit / Q000737:chemistry; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029778:Trichosanthes / Q000737:chemistry*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012503:Saponins; C488004:gualou xiebai baijiutang; D000431:Ethanol