The influence of inflammation on the expression of neuropeptides in the ileum-projecting primary sensory neurones in the pig.
In the present study the ELISA test was used to investigate the influence of chemically-induced ileitis on the dorsal root ganglia (DRG) neurons in the pig. The preliminary retrograde fluorescent tracing study revealed that ileum-projecting sensory neurones (IPN) are located in the thoracic ganglia (Th; Th8-Th13). The ileum wall in experimental (E) pigs was subjected to multiple injection with 4% paraformaldehyde to induce inflammation, while in the control (C) animals the organ was injected with 0.1 M phosphate buffer. Three days later the DRGs (Th8-Th13) collected from all the animals were evaluated for VIP, SP, CGRP, NPY, GAL and SOM content with an ELISA test. It was found that the inflammation increased clearly the tissue level of SP, GAL and SOM.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005456:Fluorescent Dyes; D005557:Formaldehyde; D019004:Galanin / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D007079:Ileitis / Q000139:chemically induced / Q000378:metabolism*; D007082:Ileum / Q000294:innervation* / Q000378:metabolism*; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D011108:Polymers; D013004:Somatostatin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D034421:Sus scrofa; D013904:Thoracic Vertebrae; D015854:Up-Regulation / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D017833:Visceral Afferents / Q000166:cytology / Q000378:metabolism*
D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D009479:Neuropeptides; D011108:Polymers; D005557:Formaldehyde; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019004:Galanin; D015740:Calcitonin Gene-Related Peptide; C003043:paraform