Beta-hemolysin-independent induction of apoptosis of macrophages infected with serotype III group B streptococcus.
Group B streptococcus (GBS) induces apoptosis in macrophages. Growth conditions minimizing beta-hemolysin expression, such as high glucose, reduce apoptosis. We constructed an isogenic mutant strain of GBS 874391 lacking the beta-hemolysin structural gene cylE and investigated the role that beta-hemolysin plays in apoptosis of J774 macrophages. Viability of macrophages infected with wild-type or cylE GBS was similar and significantly less than that of macrophages infected with GBS grown in high-glucose media. Thus, apoptosis in GBS-infected macrophages is dependent not on beta-hemolysin but on a factor coregulated with beta-hemolysin by glucose.
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001426:Bacterial Proteins; D053938:DNA Fragmentation; D005947:Glucose / Q000276:immunology / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D020287:In Situ Nick-End Labeling; D008264:Macrophages / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008854:Microscopy, Electron; D016254:Mutagenesis, Insertional; D010587:Phagocytosis; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000382:microbiology* / Q000473:pathology; D013292:Streptococcus agalactiae / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C071495:streptococcal group B hemolysin; D005947:Glucose