Development of novel microsatellite DNA markers from the Pacific oyster Crassostrea gigas.
We document the potential of novel microsatellites as a genetic tool in furthering our understanding of the Crassostrea gigas genetic structure. From the microsatellite-enriched libraries we constructed, 123 repeat regions that had sufficient sequence information to design polymerase chain reaction primer sets were isolated. From these, 9 primer pairs were screened in a C. gigas population of 67 individuals to evaluate the genetic variability. All but 1 of the 9 loci showed allelic variation (number of alleles, 2-20; observed heterozygosity, 0.119-0.925; unbiased expected heterozygosity, 0.139-0.914). Considerable discrepancy of genotypic proportions from the Hardy-Weinberg equilibrium was observed at 1 locus with an apparent heterozygote deficiency. Several loci were successfully amplified in 3 other related species with the appropriate allele size: 6 loci in C. sikamea, 4 loci in C. ariakensis, and 5 loci in C. nippona.
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D007564:Japan; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics
D017931:DNA Primers