title: Bone mass changes in vivo during the entire reproductive cycle in rats feeding different dietary calcium and calcium/phosphorus ratio content.
abstract: The purpose of this study was to quantify in vivo the impact of different dietary Ca contents on the maternal total skeleton and skeletal sub-areas in adult rats during pregnancy and lactation, using DXA. Twenty-four female Wistar rats (approximately 5 months old) were mated and divided into three groups (n = 8) and fed one of the following diets, varying only in Ca content (LCD: 0.14%, NCD: 0.6% or HCD: 1.2%). Pups were adjusted to 8-9 per dam. Maternal ionic calcium and in vivo bone mineral density (BMD) were measured at the beginning, after delivery and after weaning. Regardless of the diet, ionized calcium decreased from onset to weaning ( P < 0.05). At weaning, bone mass decreased 7.3% in NCD, 15% in LCD and 10.5% in HCD from initial values. Total skeleton, whole and proximal tibia and spine BMDs only decreased at delivery in the LCD group ( P < 0.05) but, irrespective of the diet, at weaning, they were lower compared to delivery and initial values ( P < 0.05). LCD group presented the lowest BMD in the proximal tibia and spine regions ( P < 0.05). At birth, pups did not present differences, however, at weaning, LCD pups reached the lowest body weight ( P < 0.05), NCD presented the highest body Ca content ( P < 0.05) and there were no differences between LCD and HCD. This in vivo study showed that regardless of the dietary calcium content, the maternal skeleton is slightly affected by pregnancy but severely affected by lactation. However, the degree of such response appears to depend not only on dietary Ca content but also on dietary Ca/P molar ratio.
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D002136:Calcium, Dietary / Q000008:administration & dosage* / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D007774:Lactation / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016226:Phosphorus, Dietary / Q000008:administration & dosage / Q000097:blood; D011247:Pregnancy / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002136:Calcium, Dietary; D016226:Phosphorus, Dietary