[Effect of conjunctive instillation of cholecystokinin 26-33 on pancreatic endocrine function in type 1 diabetes mellitus].
To investigate the role of cholecystokinin (ChCK) in the pathogenesis of insulin-dependent diabetes mellitus (IDDM) and to search the ways of its treatment with good prospects we conducted a comparative study of the effects of chronic conjunctive instillations (c.i.)) and intracerebroventricular administrations (i.c.v.) of cholecystokinin octapeptide (ChCK-8) to intact and streptozotocin-induced IDDM rats. The state of alpha- and beta-cells in pancreatic islets was studied by immunocytochemical method with a subsequent quantitative analysis on an automatic image analysis system. Our investigation has shown that in healthy rats both i.c.v. and c.i. administrations of ChCK-8 induced a significant increase in glycemia due to stimulation of alpha-cells and depression of beta-cells. However effects of ChCK-8 on the synthesis and secretion of insulin prevailed at i.c.v. administrations, while ChCK-8 administrated by c.i. was more potent in stimulating alpha-cells. Both ways of ChCK-8 administrations to a IDDM rats caused a positive effect on those animals by inhibiting a destruction of beta-cells, stimulating their function, and decreasing the content of alpha-cells in pancreatic islets which lead to a significant increase in insulin and a decrease in glucose in blood.
D000818:Animals; D003228:Conjunctiva / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D007276:Injections, Intraventricular; D007322:Instillation, Drug; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology*
D007328:Insulin; C044201:cholecystokinin (26-33); D012844:Sincalide