title: Oxidative transformation of phenols in aqueous mixtures.
abstract: The transformation by an oxidoreductase (a laccase from Rhus vernificera) of a mixture of four phenols (catechol, methylcatechol, m-tyrosol and hydroxytyrosol) that simulates a typical wastewater derived from an olive oil factory was investigated. Results achieved in this study confirm that laccase-mediated transformation of phenols depends on the nature and the initial concentration of the involved phenol, the time course of the reaction, and mainly, on the complexity of the phenolic incubation mixture. Actually, the four phenols each have a completely different response to enzyme action both in terms of quantitative and kinetic transformation. For example, after 24-h incubation, methylcatechol was completely removed, whereas 30% of untransformed hydroxytyrosol and catechol and more than 65% of m-tyrosol were still present in the reaction mixture. A reduction of enzyme activity occurred for all phenols after enzymatic oxidation. No correspondence between phenol transformation and disappearance of enzymatic activity was observed, thus suggesting that different mechanisms are probably involved in the laccase-mediated transformation of the four phenols. The behavior of phenols became more complex when an increasing number of phenols was present in the reaction mixture, and even more so when different concentrations of phenols were used. Competitive effects may arise when more than one phenol is present in the reaction solution and interacts with the enzyme.
mesh_terms: D042845:Laccase; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism*; D029045:Rhus / Q000201:enzymology; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000378:metabolism*
chemical_list: D010636:Phenols; D014873:Water Pollutants; D010088:Oxidoreductases; D042845:Laccase