title: Joint homeostasis. The discrepancy between old and fresh defects in cartilage repair.
abstract: The discrepancy between successful experimental studies of cartilage repair and the clinical results is unexplained. We have evaluated the effect of metabolic alterations in joint homeostasis owing to an articular defect on the outcome of cartilage repair using tissue engineering methods. We used 21 adolescent Dutch goats divided into three groups. The control knees were left untreated while the contralateral knee was randomised to receive either no treatment (N), early treatment (E) or late treatment (L). The metabolism of proteoglycans in the surrounding joint surface was determined and correlated with the O'Driscoll score used to quantify the histological aspect of the repair of the defect. Synthesis of proteoglycan (PG) was increased in all groups. The release of glucosaminoglycan (GAG) was significantly higher in the untreated but not after early transplantation (1.3 v 1.8 NS). The cartilage repair scores in the early treatment group were not as good as those of the normal control group, but were significantly better when compared with both the untreated defects and the late treated defects. Defects which had been treated late showed a significantly decreased score when compared with those which had had early treatment or the normal control group and did not differ (p = 0.12) from those with no treatment. The histological and biochemical scores closely resembled the macroscopic and functional parameters which showed a significant deterioration for the late treated group and those without treatment compared with animals treated early. Thus, tissue-engineered cartilage repair is negatively influenced by altered matrix metabolism. Early treatment showed significantly better results for repair of cartilage than late or no treatment, with a concurrent decrease in the detrimental disturbance of cartilage metabolism which constituted a protective effect on the articulation.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries / Q000378:metabolism / Q000601:surgery* / Q000637:transplantation; D004195:Disease Models, Animal; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006041:Goats; D006706:Homeostasis; D007718:Knee Injuries / Q000378:metabolism / Q000601:surgery*; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D011509:Proteoglycans / Q000096:biosynthesis; D013997:Time Factors; D023822:Tissue Engineering; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; C020341:glucosaminoglycans