Highly enhanced duplex stability of dipyrido [3,2-a:2',3'-c] phenazine-modified oligonucleotide conjugate.
Dipyrido [3,2-a:2',3'-c] phenazine (DPPZ) or 1,10-phenanthroline (Phen) was tethered to the 5'-end of a short oligonucleotide (ODN) to generate two ODN conjugates. The conjugates formed stable duplexes with complementary 6 mer (d(TTAGGG)), which is one unit of telomeric repeats of human. The melting temperature of the duplex with DPPZ conjugate was higher than that of the corresponding duplex with unmodified 6 mer by 19.6 degrees C. This stabilization is enormous compared with those observed in other ODN conjugates reported previously. It would be attributed to the effective interaction of tethered heteroaromatic groups with DNA base stack of the duplex.
D009841:Oligonucleotides / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D009841:Oligonucleotides; D010619:Phenazines; C443808:dipyrido(3,2-a-2',3'-c)phenazine