A microplate fluorimetric assay for measuring dehalogenase activity.
A fluorimetric assay was developed to measure halide release from halogenated compounds being degraded by microbes. The method relies on the property of halides to quench the fluorescence of 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) by collision quenching. The assay shows a wide response to halide concentration (1-500 mM) and tolerates a wide pH range. Furthermore, it is simple to use, has the potential for automation and uses an inexpensive non-toxic reagent.
D001673:Biodegradation, Environmental; D002138:Calibration; D002712:Chlorides / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011806:Quinolinium Compounds
D002712:Chlorides; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C052335:6-methoxy-N-(3-sulfopropyl)quinolinium