Clear distinction of pyrimidine bases on the complementary strand by fluorescence change of novel fluorescent nucleosides.
New fluorescent nucleosides, methoxybenzodeazaadenine ((MD)A) and methoxybenzodeazainosine ((MD)I) which can sharply distinguish between C and T bases, respectably. The hybridization of an ODN probe containing (MD)A and (MD)I with a target DNA facilitates the judgment with the fluorescence spectra of the type of pyrimidine bases located at a specific site on the target DNA. The (MD)A- and (MD)I containing ODN are very effective probes for pyrimidine SNP typing.
D000225:Adenine / Q000031:analogs & derivatives; D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007288:Inosine / Q000031:analogs & derivatives; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D011743:Pyrimidines; D007288:Inosine; D000225:Adenine; C030986:pyrimidine