Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent.
Reactive Black 5 industrial dyeing effluent was decolourized by free and immobilized laccase. The stability of the enzyme (194 h free and 79 h immobilized) depended on the dyeing liquor composition and the chemical structure of the dye. In the decolourization experiments with immobilized laccase, two phenomenons were observed--decolourization due to adsorption on the support (79%) and dye degradation due to the enzyme action (4%). Dyeing in the enzymatically recycled effluent provided consistency of the colour with both bright and dark dyes.
D001673:Biodegradation, Environmental; D019149:Bioreactors; D003116:Color; D003124:Colorimetry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007220:Industrial Waste / Q000517:prevention & control; D042845:Laccase / Q000737:chemistry*; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates / Q000737:chemistry*; D013783:Textile Industry / Q000379:methods*; D014876:Water Pollution / Q000517:prevention & control; D018508:Water Purification / Q000379:methods*
D004800:Enzymes, Immobilized; D007220:Industrial Waste; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates; D042845:Laccase; C063421:Remazol black B