Analysis of processing-defective variants of human tRNA(Val).
Eukaryotic tRNAs are transcribed, processed and exported from the nucleus to the cytoplasm to participate in protein biosynthesis. A variant of human gene for tRNA(Val), HtV4, is not processed neither its 5'- nor its 3'-flanking sequences. By RT-PCR analysis and in situ hybridization, we demonstrated that the HtV4 was constitutively expressed without processing, and localized in the nucleus in HeLa cells.
D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D012366:RNA, Transfer, Val / Q000737:chemistry / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012366:RNA, Transfer, Val