Chromosomal variations within aneuploid cancer lines.
Aneuploid cancers exhibit a wide spectrum of clinical aggressiveness, possibly because of varying chromosome compositions. To test this, karyotypes from the diploid CCD-34Lu fibroblast and the aneuploid A549 and SUIT-2 cancer lines underwent fluorescence in situ hybridization (FISH) and DAPI counterstaining. The number of DAPI-stained and FISH-identified chromosomes, 1-22, X,Y, as well as structural abnormalities, were counted and compared using the chi(2), Mann-Whitney rank sum test and the Levene's equality of variance. Virtually all of the evaluable diploid CCD-34Lu karyotypes had 46 chromosomes with two normal-appearing homologues. The aneuploid chromosome numbers per karyotype were highly variable, averaging 62 and 72 for the A549 and SUIT-2 lines, respectively. However, the A549 chromosome numbers were more narrowly distributed than the SUIT-2 karyotype chromosome numbers. Furthermore, 25% of the A549 chromosomes had structural abnormalities compared to only 7% of the SUIT-2 chromosomes. The chromosomal compositions of the aneuploid A549 and SUIT-2 cancer lines are widely divergent, suggesting that diverse genetic alterations, rather than chance, may govern the chromosome makeups of aneuploid cancers.
D018195:Adenosarcoma / Q000235:genetics; D000782:Aneuploidy; D002869:Chromosome Aberrations; D004171:Diploidy; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000737:chemistry; D007621:Karyotyping / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D010190:Pancreatic Neoplasms / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured
D007211:Indoles; C007293:DAPI