Potentiation of antimetastatic activity of pentoxifylline in B16F10 and B16F1 melanoma cells through inhibition of glutathione content.
OBJECTIVE
The aim of this work is to evaluate the antioxidative activity of antimetastatic agent pentoxifylline (PTX) in B16F10 and B16F1 melanoma cells.


METHODS
B16F10 and B16F1 cell lines were treated with PTX with different doses at different time intervals. Reduced glutathione, glutathione-S-transferase, and lipid peroxides were estimated to evaluate the action of PTX.


RESULTS
The activity of pentoxifylline treatment showed glutathione depletion, increased glutathione-S-transferase activity with upregulation of lipid peroxidation in B16F10 and B16F1 melanoma cells. However, the effect on GSH, GST, and LPOs was dependent on dose concentrations and time intervals.


CONCLUSIONS
Pentoxifylline, an effective membrane modifier, showed significant depletion in glutathione level in conjunction with increased lipid peroxidation. The results suggested an antimetastatic property by PTX at a nontoxic dose in B16F10 and B16F1 melanoma cells.
D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010431:Pentoxifylline / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D005982:Glutathione Transferase; D005978:Glutathione; D010431:Pentoxifylline