Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.
A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H(3) into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.
D002467:Cell Nucleus; D003562:Cytidine; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D018532:Pisum sativum; D010944:Plants / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
D009705:Nucleosides; D009711:Nucleotides; D003562:Cytidine; D012313:RNA
NUCLEOSIDES AND NUCLEOTIDES/metabolism; PLANTS/metabolism; RIBONUCLEIC ACID/metabolism