In vitro analysis of the initial steps of trans-translation.
tmRNA has a dual function both as tRNA and mRNA and facilitates trans-translation. We observed the tagging derived from tmRNA from Bacillus subtilis in vivo and in vitro. Our studies give progressive suggestions on the mechanism of trans-translation.
D001412:Bacillus subtilis / Q000235:genetics*; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics*; D014169:Transformation, Bacterial
D012329:RNA, Bacterial; C096222:tmRNA