title: Utilization efficiency of the oxidized purine nucleotide analogs by DNA polymerase eta.
abstract: To elucidate the behavior of DNA polymerase eta against the oxidized purine nucleotides, we determined the utilization efficiency of 2-hydroxy-dATP and 8-hydroxy-dGTP by the recombinant yeast DNA polymerase eta using the primer extension assay with the synthetic oligonucleotide template-primers, and compared those by DNA polymerase alpha. Results indicate that DNA polymerase eta incorporates 2-hydroxy-dATP opposite template G in addition to template T and 8-hydroxy-dGTP opposite A in addition to C, respectively. Kinetic analysis revealed that the rate of mutation caused by 2-OH-dATP and 8-OH-dGTP with DNA polymerase eta should be much higher than those with DNA polymerase alpha.
mesh_terms: D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011685:Purine Nucleotides / Q000378:metabolism*; D000099270:Y-Family DNA Polymerases
chemical_list: D004259:DNA-Directed DNA Polymerase; D011685:Purine Nucleotides; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases