Crotapotin induced modification of T lymphocyte proliferative response through interference with PGE2 synthesis.
The immunosuppressive property has been demonstrated for the venom of the Crotalus durissus terrificus. Using a simple, novel method for obtaining crotapotin and phospholipase A2 isoforms from venom, it was possible to demonstrate that the addition of crotapotin to cultures of isolated lymphocytes resulted in a significant inhibition of the cellular proliferative response to Concanavalin A. This reduction in blastogenic response of lymphocytes is accompanied by a significant increase in the production of PGE2 by macrophages. This effect on the innate immune response suggests that this compound may modify the subsequent adaptative immune response.
D000818:Animals; D003439:Crotoxin / Q000276:immunology* / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis* / Q000276:immunology; D004791:Enzyme Inhibitors / Q000276:immunology / Q000494:pharmacology; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D010741:Phospholipases A / Q000276:immunology / Q000494:pharmacology; D054467:Phospholipases A2; D013601:T-Lymphocytes / Q000187:drug effects*
D004791:Enzyme Inhibitors; D007166:Immunosuppressive Agents; D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone