Liquid chromatographic determination of alpha-solasonine in frozen green peas as an indicator of the presence of nightshade berries.
Nightshade berries containing glycoalkaloids can be a contaminant in green peas. Methodology was developed to detect this contamination. The glycoalkaloid alpha-solasonine was extracted from frozen green peas with 1% (v/v) acetic acid, cleaned up on a C18 cartridge, and determined by liquid chromatography with UV detection at 200 nm. Method performance characteristics for the determination of alpha-solasonine include linearity from 140 to 1500 ng injected (r = 0.9996-0.9999); recovery ranging from 68 to 79%; limit of quantitation (LOQ) = 4.5 ppm (280 ng standard), and limit of detection = 0.64 ppm (40 ng standard). At the LOQ, the expanded uncertainty at 95% confidence was 0.38 x the reported value. The method was applied to the detection of alpha-solasonine in frozen green peas in a 2-year study of 60 samples of frozen green peas from Ontario, Canada. None of the samples contained alpha-solasonine. No unripe berries of Solanum nigrum were detected visually in the samples.
D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005615:Freezing; D005638:Fruit / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012991:Solanaceous Alkaloids / Q000032:analysis*; D032322:Solanum / Q000737:chemistry*; D032324:Solanum nigrum / Q000737:chemistry
D012991:Solanaceous Alkaloids; C026608:alpha-solamargine