[Volume selective 31P NMR spectroscopy for differentiation of graph rejection and acute tubular necrosis after kidney transplantation].
Volume-selective 31P-MR spectra were obtained from 37 patients using a whole-body MR scanner in combination with surface coils and a modified ISIS sequence. The quantitative evaluation took place by line fitting to the signal in the time domain using a non-linear procedure. The following signal intensities were determined: PME, PDE, Pi, gamma-, alpha-, beta-ATP and pH. 1024 excitations were averaged to achieve an adequate signal-to-noise ratio (10.0 +/- 3.3), measurements lasting 34 minutes on average. The mean measured volume was 174 +/- 52.4 ml. Contamination by muscle tissue could be excluded based on the absence of phosphocreatinine signal in the spectra. Contamination by fatty tissue was excluded by visualisation. A reduction in the value of the Pi/alpha-ATP ratio as a function of time was observed due to the regeneration process following reperfusion injury. In transplant rejection (n = 7) a significant rise in Pi/alpha-ATP ratio was seen compared to the control group (n = 20) (0.4 +/- 0.16 vs. 0.22 +/- 0.11, p < 0.01), the calculated difference in pH was significant as well. In cases of acute tubular necrosis a reduced value fore the PME/PDE ratio was observed (0.65 +/- 0.1 vs. 0.96 +/- 0.5, p < 0.04). Acute tubular necrosis could be differentiated from rejection by difference in pH (6.93 +/- 0.1 vs. 7.14 +/- 0.19, p < 0.04).
D000255:Adenosine Triphosphate / Q000378:metabolism; D016477:Artifacts; D003937:Diagnosis, Differential; D004734:Energy Metabolism / Q000502:physiology*; D006084:Graft Rejection / Q000175:diagnosis* / Q000503:physiopathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016030:Kidney Transplantation / Q000502:physiology*; D007683:Kidney Tubular Necrosis, Acute / Q000175:diagnosis* / Q000503:physiopathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates / Q000378:metabolism*; D012016:Reference Values; D015427:Reperfusion Injury / Q000175:diagnosis / Q000503:physiopathology; D012680:Sensitivity and Specificity
D010710:Phosphates; D000255:Adenosine Triphosphate