title: Improving reliability of gene selection from microarray functional genomics data.
abstract: Constructing a classifier based on microarray gene expression data has recently emerged as an important problem for cancer classification. Recent results have suggested the feasibility of constructing such a classifier with reasonable predictive accuracy under the circumstance where only a small number of cancer tissue samples of known type are available. Difficulty arises from the fact that each sample contains the expression data of a vast number of genes and these genes may interact with one another. Selection of a small number of critical genes is fundamental to correctly analyze the otherwise overwhelming data. It is essential to use a multivariate approach for capturing the correlated structure in the data. However, the curse of dimensionality leads to the concern about the reliability of selected genes. Here, we present a new gene selection method in which error and repeatability of selected genes are assessed within the context of M-fold cross-validation. In particular, we show that the method is able to identify source variables underlying data generation.
mesh_terms: D000465:Algorithms; D003110:Colonic Neoplasms / Q000145:classification / Q000175:diagnosis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000145:classification* / Q000235:genetics*; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D023281:Genomics / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000175:diagnosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004273:DNA, Neoplasm