Simple preparation of biotinylated RNA by transcription via an unnatural base pair.
To develop a convenient method for RNA fragment biotinylation at a specific position, we synthesized ribonucleoside 5'-triphosphates of biotinylated unnatural bases. These unnatural bases were composed of 2-oxo(1H)pyridine (denoted as y), specifically pairing with 2-amino-6-(2-thienyl)purine (denoted as s) in transcription. The y base was linked with biotin through an allylamine or propynylamine linker at position 5 of y. These triphosphate derivatives were specifically incorporated by T7 RNA polymerase into RNA opposite s in templates. The specific biotinylation and immobilization of an RNA aptamer were demonstrated by this system.
D020029:Base Pairing; D001710:Biotin / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D014158:Transcription, Genetic
D012313:RNA; D001710:Biotin