Effects of selective prostaglandin EP2 and EP4 receptor agonists on bone resorption and formation in fetal rat organ cultures.
Prostaglandin E2 (PGE2) can stimulate bone resorption and formation through receptors which activate adenylyl cyclase. We examined the effects of selective EP2 and EP4 agonists (EP2A and EP4A) on the release of previously incorporated 45Ca from fetal rat long bones and the incorporation of [3H]-proline or [3H]-thymidine (TdR) in fetal rat calvaria to assess the relative effects of these selective agonists on bone formation and resorption. Only EP4A was effective in increasing 45Ca release. Both agonists increased [3H]-TdR incorporation and [3H]-proline incorporation into calvariae, particularly in the presence of cortisol.
D000818:Animals; D001862:Bone Resorption / Q000196:embryology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D015232:Dinoprostone / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D009924:Organ Culture Techniques; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011392:Proline / Q000378:metabolism; D051381:Rats; D018078:Receptors, Prostaglandin E / Q000819:agonists*; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D012886:Skull / Q000187:drug effects / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002132:Calcium Radioisotopes; C545646:Ptger2 protein, rat; C545652:Ptger4 protein, rat; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D014316:Tritium; D003094:Collagen; D004247:DNA; D011392:Proline; D015232:Dinoprostone; D002118:Calcium; D013936:Thymidine; D006854:Hydrocortisone