title: High frequency transformation of the Amphotericin-producing bacterium Streptomyces nodosus.
abstract: This study has investigated DNA transformation in the Amphotericin-producing organism Streptomyces nodosus. Amphotericin B is an antifungal drug with severe side effects in humans and the availability of structural variants would aid investigations into the mode of action and cytotoxity of the drug. Analogs of related polyketide drugs have been rapidly made by genetic engineering of biosynthetic genes; however, this requires the introduction of foreign DNA into the host. Protocols for protoplast formation and regeneration were established; however, preparations were recalcitrant to DNA uptake. Electroporation-mediated methodologies also were not successful. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies of 5 x 10(-5) exconjugants generated per recipient. Use of DNA methylation-impaired E. coli donor strains resulted in 100-fold higher transformation efficiencies, indicating that DNA methylation recognition systems are operable in the organism. This methodology will enable genetic and biochemical analysis of the gene cluster responsible for making Amphotericin B.
mesh_terms: D000666:Amphotericin B / Q000096:biosynthesis*; D003227:Conjugation, Genetic; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014170:Transformation, Genetic
chemical_list: D000666:Amphotericin B