Representational difference analysis: critical appraisal and method development for the identification of unique DNA sequences from prokaryotes.
Representational difference analysis (RDA) has great potential for preferential amplification of unique but uncharacterised DNA sequences present in one source such as a whole genome, but absent from a related genome or other complex population of sequences. While a few examples of its successful exploitation have been published, the method has not been well dissected and robust, detailed published protocols are lacking. Here we examine the method in detail, suggest improvements and provide a protocol that has yielded key unique sequences from a pathogenic bacterial genome.
D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D015723:Gene Library; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial