Molecular characterization of a xylanase-producing thermophilic fungus isolated from Japanese soil.
We describe the molecular characteristics of Scytalidium thermophilum isolated from Japanese soil. The S. thermophilum isolates produced higher xylanase activity than Humicola lanuginosa isolated from Japanese soil. A G/11 family xylanase-encoding gene was detected in the S. thermophilum genome by using the polymerase chain reaction technique. The S. thermophilum AF101-3 strain, which was one of the isolates in this study, grew well at 37 degrees C and 50 degrees C, and contained the maximum xylanase activity detected among the isolates. Phylogenetic analysis revealed that the S. thermophilum strains isolated from Japanese soil were clustered in a different group from the S. thermophilum strains reported by Lyons et al. [Mycol Res 104:1431, 2000], suggesting that the S. thermophilum strains isolated in this study are genetically new isolates. Therefore, the genetic diversity of S. thermophilum might be higher than that of H. lanuginosa. Moreover, this is the first report about detection of a xylanase-encoding gene in S. thermophilum.
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D006358:Hot Temperature; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase