PCR and restriction endonuclease assay for detection of a novel mutation associated with sulfonamide resistance in Neisseria meningitidis.
We identified a previously undocumented mutation in the dihydropteroate synthase (folP) gene associated with Neisseria meningitidis sulfonamide resistance. A PCR-based assay to detect this mutation, which is 100% predictive of sulfonamide resistance, was developed.
D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004094:Dihydropteroate Synthase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D009154:Mutation; D009345:Neisseria meningitidis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013449:Sulfonamides / Q000494:pharmacology*
D017931:DNA Primers; D013449:Sulfonamides; D004094:Dihydropteroate Synthase; D004262:DNA Restriction Enzymes