The extracorporeal perfusion of swine uterus as an experimental model: the effect of oxytocic drugs.
The objective of this study was to establish an experimental model for extracorporeal perfusion of swine uterus. In order to validate this model, we examined some biochemical parameters and determined the effect of oxytocic drugs (Oxytoxin, Prostaglandin E (2)) on extracorporeal perfused swine uteri. Thirty swine uteri were perfused with Krebs-Ringer bicarbonate-glucose buffer for a period up to eleven hours with the aim to preserve a viable organ, which should be responsive to hormones. The intrauterine pressure was recorded after administration of various concentrations of oxytocin and prostaglandin E (2). Perfusate pH, perfusate lactate, partial oxygen and carbon dioxide tensions, oxygen saturation, and hydrogencarbonate levels in the perfusate, all indicators of tissue ischemia or cell necrosis, showed good preservation of the organ for up to seven hours. We examined the relation of intrauterine pressure to oxytocin and prostaglandin E (2). Both were able to induce contractions of the uterus, whereas prostaglandin E (2) produced rhythmical contractions of smaller amplitude and a higher frequency. We could demonstrate that our perfusion system was able to preserve the swine uterus in a functional condition appropriate for the study of physiological questions.
D000818:Animals; D015232:Dinoprostone / Q000502:physiology*; D005260:Female; D023421:Models, Animal; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009215:Myometrium / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods; D010120:Oxytocics / Q000494:pharmacology; D010121:Oxytocin / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D013552:Swine; D014021:Tissue Preservation / Q000379:methods; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
D010120:Oxytocics; D010121:Oxytocin; D015232:Dinoprostone