Measurements of protein-protein interactions by size exclusion chromatography.
A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B(2) from positive to negative values in lysozyme and bovine serum albumin solutions. Our size exclusion chromatography results agree quantitatively with data obtained by light scattering.
D000465:Algorithms; D002850:Chromatography, Gel / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D046911:Macromolecular Substances; D009113:Muramidase / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry*
D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine; D009113:Muramidase