Comparison of different approaches for the incorporation of non-radioactive labels into polymerase chain reaction products.
Different methods for labelling polymerase chain reaction (PCR) products with non-radioactive labels for their detection by hybridization with immobilized DNA probes were compared. The use of digoxigenin (DIG) as a label provided greater sensitivity than biotin in a PCR system targeting the invA gene from Salmonella typhimurium. Incorporation of digoxigenin into amplicons in the form of 5'-DIG-labelled oligonucleotide primers resulted in better assay signals and was more economical than DIG-labelled dUTP.
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001710:Biotin; D004076:Digoxigenin; D007553:Isotope Labeling / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D001426:Bacterial Proteins; C075518:invA protein, Bacteria; D001710:Biotin; D004076:Digoxigenin