Endogenous and exogenous fibroblast growth factor-2 modulate wound healing in the chick embryo chorioallantoic membrane.
Re-epithelization and the formation of a granulation tissue consisting of inflammatory cells, newly formed blood vessels, and fibroblasts embedded in a loose collagenous extracellular matrix, are critical events occurring during wound healing. In this study, utilizing the chick embryo chorioallantoic membrane (CAM) as an in vivo model of wound healing, we investigated the role of endogenous and exogenous fibroblast growth factor-2 (FGF-2) in the wound healing reparative processes. The results showed that: (1) neutralizing anti-FGF-2 antibodies (400 ng/embryo) decreased significantly the rate of wound healing (occurring only in 25% of specimens) when applied close to the edge of the wound, causing a significant decrease of microvessel and fibroblast density, and of an inflammatory macrophage infiltrate in the wounded area; (2) conversely, the application of exogenous recombinant FGF-2 (1.0 microg/embryo) greatly accelerated the wound repair occurring approximately 24h earlier than in untreated CAMs, stimulating angiogenesis, fibroblast proliferation, and macrophage infiltration. These findings demonstrate the role of FGF-2 in wound healing of the CAM and suggest that CAM, usually employed as an in vivo assay to study angiogenesis, can also be utilized as an in vivo model for the easy, rapid, and economic screening of molecules potentially able to affect the wound healing process.