title: Distribution of type IV collagen subtypes in human testes and their association with spermatogenesis.
abstract: OBJECTIVE
  To evaluate the expression of type IV collagen [alpha1(IV) to alpha6(IV)] in testes and the association with spermatogenesis.
  
  
  METHODS
  Retrospective immunohistochemical study.
  
  
  METHODS
  Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, in a university hospital.
  
  
  METHODS
  Testicular biopsy specimens were obtained from 24 patients with varicocele, 5 with Sertoli cell-only syndrome (SCO), and 5 normal volunteers.
  
  
  METHODS
  Collection of testicular tissue and blood and semen sampling.
  
  
  RESULTS
  Expression of type IV collagen subtypes assessed by immunohistochemistry and clinical parameters such as seminogram and hormonal findings. In normal testes, the alpha1(IV) chain was seen in the basement membrane (BM) of seminiferous tubules as strongly stained irregular, wavy double lines, and the alpha2(IV) chain was slightly detected, whereas other testes showed little staining. In patients with varicocele and Sertoli cell-only syndrome, the BM was thicker and alpha1(IV) and alpha2(IV) chains were stained more intensely in the BM of seminiferous tubules than in normal testes. The expression of alpha1(IV) chain, not alpha2(IV), significantly correlated positively with the BM thickness, and negatively with sperm concentration, tubular diameter, and Johnsen score.
  
  
  CONCLUSIONS
  Overabundance of the alpha1(IV) chain is associated with increased BM thickness and possibly related to spermatogenic dysfunction.
mesh_terms: D000328:Adult; D001485:Basement Membrane / Q000378:metabolism; D001706:Biopsy; D024141:Collagen Type IV / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D011388:Prolactin / Q000378:metabolism; D012189:Retrospective Studies; D012671:Seminiferous Tubules / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D014646:Varicocele / Q000378:metabolism; D014743:Videotape Recording
chemical_list: D024141:Collagen Type IV; D013739:Testosterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone