title: Molecular recognition of proline tRNA by prolyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
abstract: To investigate the recognition mechanism of tRNA(Pro) by prolyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1, various tRNA(Pro) transcripts were prepared by in vitro transcription system. These transcripts were aminoacylated with proline by overexpressed A. pernix prolyl-tRNA synthetase. From prolylation experiments, recognition elements of A. pernix tRNA(Pro) were determined to be G35 and G36 of anticodon, discriminator base A73, and G1-C72 base pair at acceptor stem end.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D001105:Archaea / Q000201:enzymology*; D012361:RNA, Transfer, Pro / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012361:RNA, Transfer, Pro; D000604:Amino Acyl-tRNA Synthetases; C100134:prolyl T RNA synthetase