Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates.
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
D001710:Biotin / Q000737:chemistry*; D002151:Calorimetry / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019544:Equipment Failure Analysis / Q000379:methods; D046911:Macromolecular Substances; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D011126:Polypropylenes; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D046911:Macromolecular Substances; D011126:Polypropylenes; D001710:Biotin; D019809:Streptavidin