Anti-solasodine glycoside single-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants.
We constructed a recombinant antibody fragment--single chain fragment-variable (scFv) antibody--derived from hybridoma cell lines to control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The properties of the As-scFv protein expressed in Escherichia coli were almost identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root, as reflected by the soluble As-scFv level and antigen binding activities. These results suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level, thus representing a novel plant breeding methodology that can produce secondary metabolites.
D000595:Amino Acid Sequence; D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006027:Glycosides / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D006825:Hybridomas; D007128:Immunoglobulin Fragments / Q000276:immunology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012991:Solanaceous Alkaloids / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D032322:Solanum / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D006027:Glycosides; D007128:Immunoglobulin Fragments; D010940:Plant Proteins; D011994:Recombinant Proteins; D012991:Solanaceous Alkaloids; C055966:immunoglobulin Fv; C026609:beta-solamarine; C012037:solasodine