Expression, purification, crystallization and preliminary crystallographic analysis of the calponin-homology domain of Rng2.
Rng2 is a multidomain protein component of the actiomyosin ring and the spindle pole body necessary for cytokinesis in Schizosaccharomyces pombe. The calponin-homology domain of Rng2 from S. pombe has been overexpressed, purified and crystallized. The crystals belong to space group P2(1). Br- and Hg-derivative data sets were measured to 2.21 A using synchrotron radiation from crystals that were partially fixed with glutaraldehyde. Electron-density maps have been obtained from two-wavelength MAD on the Br derivative and SAD on the Hg derivative.
D002135:Calcium-Binding Proteins / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D020690:GTPase-Activating Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D017356:Synchrotrons; D013917:Thrombin / Q000378:metabolism; D000096985:Calponins
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D020690:GTPase-Activating Proteins; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D013917:Thrombin; D000096985:Calponins; C114172:Rng2 protein, S pombe