Altered expression of connective tissue genes in postnatal chondroinduced human dermal fibroblasts.
In a novel model for postnatal chondroinduction, normal human dermal fibroblasts (hDFs) cultured with demineralized bone powder (DBP) express chondrocyte features after 7 days. Representational difference analysis (RDA) prior to overt chondroblastogenesis (3 days) revealed altered expression of connective tissue genes (collagens, collagen receptors, and post-translational enzymes). Northern or RT-PCR analysis at 3, 7, 14, or 21 d showed different expression patterns for those genes. COL3A1 was transiently upregulated, whereas upregulation of COL11A1, integrin alpha-11, lysyl oxidase, and lysyl hydroxylase 2 mRNAs persisted for 7 days. Downregulation of decorin was sustained for 21 d. The expression of the post-translational enzymes induced by DBP was unique when compared with human skin and human articular chondrocytes. Thus, the data suggest an "induced chondroblast" stage with a unique connective tissue gene expression profile that may result in a matrix supportive of chondrogenesis by postnatal cells.
D000368:Aged; D002356:Cartilage / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics; D024161:Collagen Type XI / Q000235:genetics; D003238:Connective Tissue / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D058575:Decorin; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D016326:Extracellular Matrix Proteins; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D039001:Integrin alpha Chains / Q000235:genetics; D008297:Male; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics; D011509:Proteoglycans / Q000235:genetics; D038981:Receptors, Collagen / Q000235:genetics; D012038:Regeneration / Q000187:drug effects / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
C439971:COL3A1 protein, human; D024061:Collagen Type III; D024161:Collagen Type XI; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; C121535:ITGA11 protein, human; D039001:Integrin alpha Chains; D011509:Proteoglycans; D038981:Receptors, Collagen; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D008249:Protein-Lysine 6-Oxidase