title: A simple, low-cost device for processing and embedding tissues at sub-zero temperatures.
abstract: A simple apparatus to maintain tissues samples at sub-zero temperatures during dehydration, infiltration, and polymerization is described. The device uses a conventional siphon-type carbon dioxide gas cylinder to maintain an aluminum block at temperatures as low as -35 degrees C for over 15 hours/cylinder.
mesh_terms: D000818:Animals; D003080:Cold Temperature; D005612:Freeze Drying / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D005615:Freezing; D006652:Histological Techniques / Q000295:instrumentation; D007082:Ileum; D051379:Mice; D013696:Temperature; D016610:Tissue Embedding / Q000379:methods*