Molecular design of hairpin pyrrole-imidazole polyamides possessing sequence specific DNA alkylating moiety.
By conjugating pyrrole (Py)/imidazole (Im) hairpin polyamides with CBI, a stable alkylating moiety, we have developed a new type of DNA-sequence-specific alkylating agent. The sequence specificity and alkylation efficiency of the CBI conjugates were analyzed with high-resolution denaturing gel electrophoresis using linear 450- and 1000-bp DNA. The results demonstrate that CBI conjugates selectively alkylate adenine in specific sequences, whereas the previous type of alkylating polyamide, which contains segment A of DU-86, alkylates both adenine and guanine.
D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; D004247:DNA