title: Evidence for the existence of an oligomeric, non-DNA-binding complex of the progesterone receptor in the cytoplasm.
abstract: Steroid receptors are found as a hetero-oligomeric complex in cell extracts. Due to the dynamic interaction between receptor-associated proteins and receptors, it is difficult to study the oligomeric complex in living cells. Here this was attempted in cells in which the interaction was stabilized by introducing molybdate into the cells or by incubating the cells at low temperature. The complex was studied with an antibody (aD) recognizing only the dissociated form of the chicken progesterone receptor (PR) and with antibodies (PR22, PR6). Recognizing also oligomeric forms of the receptor. When wild-type chicken PR was transfected, all antibodies showed nuclear staining. Molybdate or cold treatment of cells resulted in cytoplasmic accumulation of the PR as detected with PR22/PR6. aD, however, stained predominantly the nuclear PR in treated cells. These findings suggest that when the oligomeric complex of the PR is stabilized in intact cells in vivo and then crosslinked with paraformaldehyde, a portion of the cytoplasmic receptor is seen as an oligomeric complex, whereas, in the nucleus, most, if not all receptor molecules are in dissociated form.
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D046911:Macromolecular Substances; D018613:Microscopy, Confocal; D008982:Molybdenum / Q000494:pharmacology; D011980:Receptors, Progesterone / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D046911:Macromolecular Substances; D011980:Receptors, Progesterone; C044659:molybdate; D008982:Molybdenum; D004247:DNA