[Molecular cloning and identification of calmegin gene in embryo and adult testes by cDNA microarray hybridization].
OBJECTIVE
To identify gene differential expression in embryo and adult testes by cDNA microarray techniques.


METHODS
cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed.


RESULTS
The calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes.


CONCLUSIONS
Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.
D000328:Adult; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000196:embryology / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D018832:Molecular Chaperones; C087341:calmegin