A direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro.
A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA