title: Functional role of granule-associated genes, phaP and phaR, in poly-beta-hydroxybutyrate biosynthesis in recombinant E. coli harboring phbCAB operon.
abstract: The expression characteristic of two granule-associated genes, phaP and phaR, in a recombinant E. coli harboring the phbCAB operon was investigated. Polybetamydroxybutyrate (PHB) accumulation increased up from 16% to 57% (w/w) after transformation of the granule-associated genes due to the stabilization of PHB granules rather than by a direct effect on PHB biosynthetic enzymes. The morphology of PHB granules also varied depending on the transformed phaP and phaR genes.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020118:Cupriavidus necator / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013499:Surface Properties; D000094122:Polyhydroxybutyrates
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D011994:Recombinant Proteins; D012097:Repressor Proteins; C118828:PHAP protein, Bacteria; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates