Production of crocin using Crocus sativus callus by two-stage culture system.
Saffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate (300 mg l(-1)) at 22 degrees C in dark with naphthalene acetic acid (2 mg l(-1)) and 6-benzyladenine (1 mg l(-1)) to give maximum biomass (16 g dry wt l(-1)), and with indole 3-acetic acid (2 mg l(-1)) and 6-benzyladenine (0.5 mg l(-1)) for crocin formation. The maximum crocin production (0.43 g l(-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method.
D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D027622:Crocus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D003583:Cytokinins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D007210:Indoleacetic Acids / Q000494:pharmacology; D008027:Light; D013696:Temperature
D003470:Culture Media; D003583:Cytokinins; D007210:Indoleacetic Acids; D002338:Carotenoids; C029036:crocin