Xanthofulvin, a novel semaphorin inhibitor produced by a strain of Penicillium.
A new semaphorin inhibitor xanthofulvin was isolated from the cultured broth of a fungus Penicillium sp. SPF-3059 along with a known compound vinaxanthone by solvent extraction and bioassay-guided fractionation. The tautomeric structure of xanthofulvin was determined by spectroscopic analyses. The two compounds exhibited significant semaphorin inhibitory activity with IC50 values of 0.09 and 0.1 microg/ml, respectively, in semaphorin3A-induced growth cone collapse assay using cultured chick dorsal root ganglia neurons.
D000818:Animals; D002642:Chick Embryo; D002867:Chromones; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005285:Fermentation; D005727:Ganglia, Spinal / Q000187:drug effects / Q000201:enzymology; D020439:Growth Cones / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010407:Penicillium / Q000737:chemistry / Q000378:metabolism*; D040121:Semaphorin-3A / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014966:Xanthenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D044004:Xanthones
D002867:Chromones; D004791:Enzyme Inhibitors; D040121:Semaphorin-3A; D014966:Xanthenes; D044004:Xanthones; C477901:xanthofulvin