Expression of hNP22 is altered in the frontal cortex and hippocampus of the alcoholic human brain.
BACKGROUND
Human neuronal protein (hNP22) is a gene with elevated messenger RNA expression in the prefrontal cortex of the human alcoholic brain. hNP22 has high homology with a rat protein (rNP22). These proteins also share homology with a number of cytoskeleton-interacting proteins.


METHODS
A rabbit polyclonal antibody to an 18-amino acid epitope was produced for use in Western and immunohistochemical analysis. Samples from the human frontal and motor cortices were used for Western blots (n = 10), whereas a different group of frontal cortex and hippocampal samples were obtained for immunohistochemistry (n = 12).


RESULTS
The hNP22 antibody detected a single protein in both rat and human brain. Western blots revealed a significant increase in hNP22 protein levels in the frontal cortex but not the motor cortex of alcoholic cases. Immunohistochemical studies confirmed the increased hNP22 protein expression in all cortical layers. This is consistent with results previously obtained using Northern analysis. Immunohistochemical analysis also revealed a significant increase of hNP22 immunoreactivity in the CA3 and CA4 but not other regions of the hippocampus.


CONCLUSIONS
It is possible that this protein may play a role in the morphological or plastic changes observed after chronic alcohol exposure and withdrawal, either as a cytoskeleton-interacting protein or as a signaling molecule.
D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000235:genetics* / Q000473:pathology; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000633:toxicity; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009044:Motor Cortex / Q000187:drug effects / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009473:Neuronal Plasticity / Q000235:genetics; D017397:Prefrontal Cortex / Q000187:drug effects / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D016415:Sequence Alignment; D013375:Substance Withdrawal Syndrome / Q000235:genetics / Q000473:pathology
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C422902:TAGLN3 protein, human; C486029:Tagln3 protein, rat; D000431:Ethanol