[Expression and clinical significance of p27(kip1), p16 and proliferating cell nuclear antigen in nasopharyngeal carcinoma].
OBJECTIVE
To evaluate the roles of p27(kip1), p16 gene protein and proliferating cell nuclear antigen expression in nasopharyngeal carcinoma (NPC).


METHODS
The EnVision immunohistochemical method was used to detect the expression of p27(kip1), p16 gene protein and PCNA in 66 cases of non-keratinized carcinoma (NKC) and 25 cases of non-tumor nasopharyngeal tissue.


RESULTS
(1) The positive expression rates of p27(kip1), p16 gene protein were 65%, 68% in NKC respectively. There were significant differences between NKC and non-tumor group (P < 0.05). (2) The expression of p27(kip1), p16 protein correlated with cranial nerve encroaching and the 5-year survival rates of the patients (P < 0.05), but had no significant correlation to lymph node metastases and clinical staging (P > 0.05). The expression of PCNA was related to clinical staging and to the patient's 5-year survival rates (P < 0.05), but not to lymph node metastases and cranial nerve encroaching (P > 0.05). (3) The positive expression of p27(kip1), p16 gene protein and PCNA were correlated.


CONCLUSIONS
The results suggest that immunological labeling of p27(kip1), p16 gene protein and PCNA might be used to determine the prognosis of NKC.
D000328:Adult; D000368:Aged; D018797:Cell Cycle Proteins / Q000032:analysis*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000032:analysis*
D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018809:Proliferating Cell Nuclear Antigen; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27