Photodynamic antisense therapy: regulation of cervical carcinoma cells by psoralen-conjugated oligonucleotides.
To reinforce antisense effects, we focused on the photo-cross-linking ability of psoralen derivatives and examined the applicability of the photodynamic antisense therapy to cancer cells using psoralen-conjugated oligo(nucleosides phosphorothioate)s (Ps-S-Oligo). The regulatory effects of Ps-S-Oligos on the proliferation of cervical carcinoma were evaluated. The inhibitory effects were found to be significant (IC50=16 nM) without any drug delivery systems, and it was also found that the mechanism was associated with the p53 induced apoptosis. This result suggests that the photodynamic antisense therapy will be a promising concept for the cancer therapy.
D017209:Apoptosis; D002455:Cell Division; D005260:Female; D011564:Furocoumarins / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D020287:In Situ Nick-End Labeling; D009841:Oligonucleotides / Q000737:chemistry* / Q000627:therapeutic use; D010778:Photochemotherapy; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology*
D011564:Furocoumarins; D009841:Oligonucleotides