The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis.
The three genomic RNAs of alfalfa mosaic virus each contain a unique 5' untranslated region (5' UTR). Replacement of the 5' UTR of RNA 1 by that of RNA 2 or 3 yielded infectious replicons. The sequence of a putative 5' stem-loop structure in RNA 1 was found to be required for negative-strand RNA synthesis. A similar putative 5' stem-loop structure is present in RNA 2 but not in RNA 3.
D020121:5' Untranslated Regions / Q000502:physiology*; D017794:Alfalfa mosaic virus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000096:biosynthesis* / Q000737:chemistry; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D014779:Virus Replication
D020121:5' Untranslated Regions; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase