title: Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus.
abstract: Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D002448:Cell Adhesion; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009154:Mutation; D010641:Phenotype; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins