Validation of screening method for residues of diethylstilbestrol, dienestrol, hexestrol, and zeranol in bovine urine using immunoaffinity chromatography and gas chromatography/mass spectrometry.
A method was developed, using commercially available immunoaffinity chromatography cleanup cartridges, followed by detection by gas chromatography/mass spectrometry, to screen for residues of the hormone growth promotants diethylstilbestrol, dienestrol, hexestrol, and zeranol in bovine urine. The single-laboratory, in-house validation included assessment of recoveries, repeatability, linearity of response, detection capability, and specificity (cross-reactivity) with a suite of antibiotics and other hormonal growth promotants. The method was validated for screening at a target concentration of 2.0 microg/L in urine. The detection capabilities for the analytes were diethylstilbestrol, 0.24; dienestrol, 0.15; hexestrol, 0.84; and zeranol, 0.28 microg/L.
D000818:Animals; D002417:Cattle / Q000652:urine*; D002846:Chromatography, Affinity / Q000379:methods*; D004028:Dienestrol / Q000652:urine; D004054:Diethylstilbestrol / Q000652:urine; D004350:Drug Residues / Q000032:analysis; D004968:Estrogens, Non-Steroidal / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006589:Hexestrol / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015029:Zeranol / Q000652:urine
D004968:Estrogens, Non-Steroidal; D006589:Hexestrol; D004054:Diethylstilbestrol; D015029:Zeranol; D004028:Dienestrol