Separation and determination synchronously by multichannel mode-filtered light capillary electrochromatography.
Mode-filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 micro m) and a bovine serum albumin (BSA)-modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol-gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT-IR). The separation of DL-tryptophan (10 mM) was investigated under different voltage/current conditions.
D000818:Animals; D002021:Buffers; D002417:Cattle; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005336:Fiber Optic Technology; D005374:Filtration; D005782:Gels; D008027:Light; D018625:Microscopy, Atomic Force; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry
D002021:Buffers; D005782:Gels; D012821:Silanes; D012710:Serum Albumin, Bovine; C040733:tetraethoxysilane; D014364:Tryptophan