Recovery of infectious foot-and-mouth disease virus from suckling mice after direct inoculation with in vitro-transcribed RNA.
We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147-->P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.
D000595:Amino Acid Sequence; D000818:Animals; D000833:Animals, Suckling; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D051379:Mice; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000633:toxicity*; D014158:Transcription, Genetic; D014774:Virulence; D014779:Virus Replication
D012367:RNA, Viral