Plasma steroid and nutrient levels during the active season in wild Testudo horsfieldi.
Plasma concentrations of sex steroids (testosterone and progesterone), proteins (total protein and albumin), lipids (phospholipids, cholesterol, and triglycerides), and minerals (calcium and phosphorus) were measured in wild Testudo horsfieldi in Uzbekistan, during the short, 3-month activity period (March-May, 1998). Testosterone concentration in males was highest (52 ng/ml) when they had just emerged from brumation (hibernation) in mid-March, which was also the beginning of the mating period, and fell in April. In females, progesterone peaked in mid-April (at 10 ng/ml), just before ovulation of the first clutches at the end of April and beginning of May. Testosterone levels in females and progesterone levels in males were low (<3 ng/ml) throughout the activity period. In general, the plasma concentrations of proteins, lipids, and phosphorus increased slowly in males, but more rapidly in females, during the activity season. These increases were particularly strong in females in the second half of April, coinciding with the peaks in female hormone levels. The changes in plasma hormones and nutrients reflected the timing of the different behaviours. The four first weeks of above-ground activity (mid-March to mid-April) by males, when they had high testosterone levels, were primarily allocated to fighting other males, courtship, and mating, while females spent much of that time feeding. Thereafter, both sexes concentrated on feeding. Females were probably preparing to ovulate in late-April, when their progesterone levels were highest and when plasma nutrient levels increased considerably.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002118:Calcium / Q000097:blood; D005260:Female; D006605:Hibernation / Q000502:physiology; D008055:Lipids / Q000097:blood; D008297:Male; D009752:Nutritional Status / Q000502:physiology*; D010758:Phosphorus / Q000097:blood; D011374:Progesterone / Q000097:blood*; D012098:Reproduction / Q000502:physiology*; D012621:Seasons; D012727:Sex Characteristics; D013739:Testosterone / Q000097:blood*; D014426:Turtles / Q000378:metabolism*
D001798:Blood Proteins; D008055:Lipids; D010758:Phosphorus; D013739:Testosterone; D011374:Progesterone; D002118:Calcium