2-ketogluconic acid production and phosphate solubilization by Enterobacter intermedium.
Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200-250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period.
D003470:Culture Media; D004754:Enterobacter / Q000302:isolation & purification / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology; D012988:Soil Microbiology; D012995:Solubility
D003470:Culture Media; D005942:Gluconates; D010710:Phosphates; C008229:2-ketogluconate