title: Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis.
abstract: OBJECTIVE
  To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA).
  
  
  METHODS
  Controlled experimental study.
  
  
  METHODS
  The animal laboratory of the Experimental Surgery Center of Ege University, Izmir, Turkey.
  
  
  METHODS
  Ten nondiabetic (control) and 20 diabetic adult male albino rats.
  
  
  METHODS
  The rats were IP injected with 40 mg/kg streptozotocin for 5 days to create a diabetic condition that was confirmed by testing blood glucose levels 5-7 days after the first injection.
  
  
  METHODS
  Mean testicular diameter, mean semniferous tubuli diameter (MSTD), and PCNA index.
  
  
  RESULTS
  Six weeks after the streptozotocin injection, both the mean testicular and the seminiferous tubuli diameters were significantly decreased in diabetic rats compared with the control group.
  
  
  CONCLUSIONS
  The effects of diabetes on spermatogenesis can be clearly detected as a decrease in testicular diameter, MSTD, and PCNA index.
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D012671:Seminiferous Tubules / Q000473:pathology*; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D018809:Proliferating Cell Nuclear Antigen