[Study on cyto-compatibility of PGLA film for periodontal guided tissue regeneration].
This study was aimed to evaluate the cyto-compatibility of PGLA film for periodontal guided tissue regeneration (GTR) and their degradable products. Different extraction temperature, time and ratio were used to assess the cell relative growth rate (RGR) for PGLA. The degradable solution were brought into contact with cultured cells in vitro to observe the effects of cytotoxicity at 2, 4, 6, 8 and 10 weeks. The results showed when the extraction ratio was 0.1 g/ml, the extraction time has no effects on cytotoxicity at 37 degrees C. When the ratio was 0.1 cm2/ml, slight cytotoxic reaction appeared with the increase of extraction temperature (50 degrees C or 70 degrees C). When the extraction ratio was 0.5 cm2/ml in contract with the degradable solution at 37 degrees C for 72 h, the cell growth rate decreased. When the ratio was 6 cm2/ml, the cytotoxicity existed in some degree even if the temperature was at 37 degrees C for 24 h. After 2-4 w, the degradable solution had no obvious toxic effects on cells. However, the RGR gradually decreased as the degradation period increased. In conclusion, the effects of the extraction temperature, time, ratio and the degradation products accumulating in solution on RGR may exist. PGLA film has a good cytocompatibility.
Sun
Jiao
J
Ninth People's Hospital, Shanghai Second Medical University, Shanghai Biomaterials Research & Testing Center, Shanghai 200023. jiaosun59@yahoo.com
He
Wei
W
Meng
Aiying
A
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration; D008422:Materials Testing; D051379:Mice
D023181:Antimicrobial Cationic Peptides; C045866:peptide-Gly-Leu-amide