title: Heterologous diploid nuclei in the violet root rot fungus, Helicobasidium mompa.
abstract: Allelic genes hga1-1 and hga1-2, which encode G protein alpha subunit in the violet root rot fungus, Helicobasidium mompa, were sequenced and characterized. Restriction fragment polymorphism (RFLP) analysis determined that the gene is present as a single locus in the single basidiospore isolates, while strain V169 possessed both alleles of this gene. Therefore, although basidiospore isolates are dikaryon, they are homokaryotic. Field-isolated strain V169, the putative parent strain, is a dikaryotic heterokaryon. Allelic genes hga1-1 and hga1-2 segregated in almost a 1:3 ratio among single basidiospore isolates from the same fruiting body. Moreover, the copy number of hga1-1 was found to be less than that of hga1-2 in the V169 strain. These results suggest that one of the nuclei in the V169 parent strain is homozygous diploid and the other heterozygous diploid. This parent strain produced four homokaryotic and dikaryon basidiospores on each basidium.
authors.affiliation: Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan.
authors.forename: Tadanori
authors.initials: T
authors.lastname: Aimi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Sanae
authors.initials: S
authors.lastname: Kano
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yotsutani
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Morinaga
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004171:Diploidy; D005656:Fungal Proteins / Q000235:genetics; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics; D005800:Genes, Fungal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D044385:GTP-Binding Protein alpha Subunits