Apoptosis in male germ cells in response to cyclin A1-deficiency and cell cycle arrest.
Male mice homozygous for a mutated allele of the cyclin A1 gene (Ccna1) are sterile due to a block in cell cycle progression before the first meiotic division. Meiosis arrest in Ccna1(-/-) spermatocytes is associated with desynapsis abnormalities, lowered MPF activity, and apoptosis as evidenced by TUNEL-positive staining. With time, adult testicular tubules exhibit severe degeneration: some tubules in the older animals are almost devoid of germ cells at various stages of spermatogenesis. The mechanisms by which the cells sense the cell cycle arrest and the regulation of the decision to undergo cell death are under investigation.
Salazar
Glicella
G
Department of Genetics & Development, Institute of Human Nutrition, Center for Reproductive Sciences, College of Physicians & Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Liu
Dong
D
Liao
Ching
C
Batkiewicz
Leah
L
Arbing
Rachel
R
Chung
Sanny S W
SS
Lele
Karen
K
Wolgemuth
Debra J
DJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology; D019925:Cyclin A / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D056750:Cyclin A1; D005854:Germ Cells / Q000473:pathology*; D008297:Male; D000090204:Mesothelin; D051379:Mice; D016296:Mutagenesis; D013091:Spermatogenesis / Q000502:physiology
C503747:Ccna1 protein, mouse; D019925:Cyclin A; D056750:Cyclin A1; C000715528:Msln protein, mouse; D000090204:Mesothelin