[In vitro model system of transepithelial migration of neutrophil by Helicobacter pylori].
OBJECTIVE
Migration of neutrophil across the intestinal epithelium is a structural hallmark of active intestinal diseases. One of the characteristic histologic features of Helicobacter pylori (H. pylori) infection is neutrophil infiltration of the gastric mucosa. This study was aimed to establish an in vitro model system of transepithelial migration of neutrophil by H. pylori.


METHODS
Caco-2 cells were grown on polycarbonate membrane. Confluence and integrity were assessed by measuring the transepithelial electrical resistance (TER) and paracellular permeability of macromolecular marker. IL-8 mRNA expression was assessed after H. pylori infection by quantitative RT-PCR. Isolated neutrophils and H. pylori were applied to the basolateral and apical compartment in separate. Also neutrophil transmigration was assessed by light and electron microscopy.


RESULTS
Paracellular leakage of 3[H]-mannitol was negligible over TER 166 Omega x cm2. IL-8 mRNA expression increased after H. pylori infection. Neutrophil transmigration was increased by adding of N-formyl-methionyl-leucyl-phenylalanine or H. pylori.


CONCLUSIONS
This in vitro model system could be applied to the study of pathogenesis of H. pylori infection. A further study of quantification of transmigrated neutrophils is demanded to reach a confirmation.
Jung
Hyun Chae
HC
Department of Internal Medicine, Chung Ang University College of Medicine, Jung-gu, Seoul, Korea.
Kim
Jae Gyu
JG
Kim
Jung Mogg
JM
Song
In Sung
IS
Park
Sill Moo
SM
D018938:Caco-2 Cells; D002465:Cell Movement; D002478:Cells, Cultured; D017097:Electric Impedance; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology*; D016481:Helicobacter Infections / Q000378:metabolism / Q000473:pathology*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D008567:Membranes, Artificial; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D010539:Permeability; D011076:Polycarboxylate Cement; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016209:Interleukin-8; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; D012333:RNA, Messenger; C005506:polycarbonate; D009240:N-Formylmethionine Leucyl-Phenylalanine