title: Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling.
abstract: The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C(2)-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C(2)-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C(2)-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.
authors.affiliation: Center for Basic Neuroscience, Department of Molecular Genetics, The University of Texas Southwestern Medical Center, Dallas, TX 75390-9111, USA.
authors.forename: Tuhin
authors.initials: T
authors.lastname: Virmani
authors.forename: Weiping
authors.initials: W
authors.lastname: Han
authors.forename: Xinran
authors.initials: X
authors.lastname: Liu
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Südhof
authors.forename: Ege T
authors.initials: ET
authors.lastname: Kavalali
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D000831:Animals, Newborn; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D014644:Genetic Variation; D006624:Hippocampus; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D013569:Synapses / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure*; D050857:Synaptotagmins
chemical_list: D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C494304:Syt7 protein, rat; D050857:Synaptotagmins