title: Use of a novel carbon fibre composite material for the femoral stem component of a THR system: in vitro biological assessment.
abstract: A novel, low elastic modulus femoral component for THR has been developed using a composite of polyetheretherketone and carbon fibre. The objectives of this study were to investigate human osteoblast-like cell and macrophage responses to this material in vitro. Cells were grown on composite discs and controls. Osteoblast attachment and proliferation was not significantly different to that on Ti6Al4V. The levels of alkaline phosphatase activity, Type I collagen production and osteocalcin production were not significantly different to that on Ti6Al4V by the end of the experimental period. Hydrogen peroxide production by macrophages was significantly less than that detected for cells cultured on copper, but was still greater than that detected for cells cultured on tissue culture plastic and Ti6Al4V. Beta-glucoronidase activity was not significantly different to that detected for cells cultured on tissue culture plastic. The in vitro biocompatibility assessment of this composite undertaken in this study showed initial osteoblast attachment at least comparable to that of the tissue culture plastic and Ti6Al4V controls, with proliferation similar to the controls at all time points up to 11 days. Alkaline phosphatase activity was similar to that of Ti6Al4V but reduced compared to tissue culture plastic controls. Whilst hydrogen peroxide production by macrophages was raised on composite surfaces compared to controls, beta-glucoronidase activity and osteoblastic production of Type I collagen and osteocalcin were similar to levels detected on Ti6Al4V.
authors.affiliation: School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2RD, UK. colin.scotchford@nottingham.ac.uk
authors.forename: Colin A
authors.initials: CA
authors.lastname: Scotchford
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Garle
authors.forename: J
authors.initials: J
authors.lastname: Batchelor
authors.forename: John
authors.initials: J
authors.lastname: Bradley
authors.forename: David M
authors.initials: DM
authors.lastname: Grant
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002244:Carbon; D000077482:Carbon Fiber; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D019544:Equipment Failure Analysis; D005269:Femur / Q000601:surgery; D006622:Hip Prosthesis; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D008420:Manufactured Materials; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D000077482:Carbon Fiber; D024042:Collagen Type I; D015675:Osteocalcin; D002244:Carbon; D000469:Alkaline Phosphatase