title: Exploratory and confirmatory gene expression profiling of mac1Delta.
abstract: Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Delta. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1.
authors.affiliation: Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA.
authors.forename: Jeane Maria
authors.initials: JM
authors.lastname: De Freitas
authors.forename: Jeung Hyoun
authors.initials: JH
authors.lastname: Kim
authors.forename: Helen
authors.initials: H
authors.lastname: Poynton
authors.forename: Trent
authors.initials: T
authors.lastname: Su
authors.forename: Henri
authors.initials: H
authors.lastname: Wintz
authors.forename: Tama
authors.initials: T
authors.lastname: Fox
authors.forename: Patty
authors.initials: P
authors.lastname: Holman
authors.forename: Alex
authors.initials: A
authors.lastname: Loguinov
authors.forename: Sunduz
authors.initials: S
authors.lastname: Keles
authors.forename: Mark
authors.initials: M
authors.lastname: van der Laan
authors.forename: Chris
authors.initials: C
authors.lastname: Vulpe
mesh_terms: D001483:Base Sequence; D003300:Copper / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D007501:Iron / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; C084954:MAC1 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper; D007501:Iron