A novel and convenient method for the synthesis of free 5'-thiol modified oligonucleotides.
The synthesis of free 5'-thiol-modified oligonucleotides using a 4,4',4''-trimethoxytrityl (TMTr)-protected linker and standard Poly-Pak purification has been described.
Kupihár
Z
Z
Nucleic Acids Laboratory, Department of Medicinal Chemistry, University of Szeged, Szeged, Hungary. kupi@ovrisc.mdche.u-szeged.hu
Kovács
G
G
Kele
Z
Z
Darula
Z
Z
Kovács
L
L
D001483:Base Sequence; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D013438:Sulfhydryl Compounds