Bioreduction of prochiral ketones with yeast cells cultivated in a vibrating air-solid fluidized bed fermentor.
A brief review of fluidized bed fermentors and of bioreduction of prochiral ketones by yeast cells is presented. Cultivation of yeast cells, Saccharomyces cerevisiae HUT 7099, in a vibrating fluidized bed and the bioreduction of ethyl acetoacetate by the cells are described. The cultivation of the cells in the fermentor was successfully performed at relatively low moisture content, about 40 % on wet basis. The cell size decreased and the shape changed from ellipsoid to spherical after the logarithmic growth phase. The biocatalytic performance of yeast cells cultivated in submerged, static solid, and fluidized bed cultures was compared. The cells cultivated in static solid culture exhibited the highest activity. Possible accumulation of energy sources by the cells was suggested as the explanation for better performance.
Matsuno
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R
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Adachi
S
S
Uosaki
H
H