title: Construction of a homologous selectable marker gene for Lentinula edodes transformation.
abstract: We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
authors.affiliation: Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Irie
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Sato
authors.forename: Kumiko
authors.initials: K
authors.lastname: Saito
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Enei
mesh_terms: D015139:Blotting, Southern; D002261:Carboxin / Q000494:pharmacology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005822:Genetic Vectors; D007506:Iron-Sulfur Proteins / Q000235:genetics; D017354:Point Mutation; D021122:Protein Subunits; D020942:Shiitake Mushrooms / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D007506:Iron-Sulfur Proteins; D021122:Protein Subunits; D002261:Carboxin; D004262:DNA Restriction Enzymes