Alteration in the metastatic potential of ovarian cancer cells by transfection of the antisense gene of beta-1,4-galactosyltransferase.
beta-1,4-galactosyltransferase (beta-1,4-GT) has been reported to be activated in ovarian carcinoma cells and an isoform of this enzyme has been used as a tumor marker for ovarian cancer. The present study was undertaken to clarify how beta-1,4-GT affected the cell biological characteristics of ovarian cancer. To this end, we transfected an ovarian tumor cell line with an antisense gene of beta-1,4-GT. Proliferative potential and morphology of the cells transfected with the antisense gene did not differ from those of the control cells. Adhesive potential to the constituents of extracellular matrix was reduced in the antisense gene transfectants. In a nude mouse, the number of peritoneal dissemination foci of the antisense transfectants was smaller than that of the control cells. These results indicated that beta-1,4-GT is closely related to the invasive and metastatic potentials of ovarian cancer while it is not involved in the proliferative potential.
Yamashita
Hiroshi
H
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo 160-8582, Japan. yama2397@mc.neweb.ne.jp
Kubushiro
Kaneyuki
K
Ma
Jun
J
Fujii
Takuma
T
Tsukazaki
Katsumi
K
Iwamori
Masao
M
Nozawa
Shiro
S
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002448:Cell Adhesion; D002455:Cell Division; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009237:N-Acetyllactosamine Synthase / Q000235:genetics*; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000235:genetics*; D020033:Protein Isoforms; D014162:Transfection
D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; D009237:N-Acetyllactosamine Synthase