title: Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs.
abstract: The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase. More recent work suggested a role of Hfq in cellular physiology through its interaction with ompA mRNA and small RNAs (sRNAs), some of which are involved in translational regulation. Despite their stability under certain conditions, E. coli sRNAs contain putative RNase E recognition sites, that is, A/U-rich sequences and adjacent stem-loop structures. We show herein that an RNase E cleavage site coincides with the Hfq-binding site in the 5'-untranslated region of E. coli ompA mRNA as well as with that in the sRNA, DsrA. Likewise, Hfq protects RyhB RNA from in vitro cleavage by RNase E. These in vitro data are supported by the increased abundance of DsrA and RyhB sRNAs in an RNase E mutant strain as well as by their decreased stability in a hfq(-) strain. It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack.
authors.affiliation: Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria. ISA@GEM.UNIVIE.AC.AT
authors.forename: Isabella
authors.initials: I
authors.lastname: Moll
authors.forename: Taras
authors.initials: T
authors.lastname: Afonyushkin
authors.forename: Oresta
authors.initials: O
authors.lastname: Vytvytska
authors.forename: Vladimir R
authors.initials: VR
authors.lastname: Kaberdin
authors.forename: Udo
authors.initials: U
authors.lastname: Bläsi
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D004722:Endoribonucleases / Q000378:metabolism*; D035001:Host Factor 1 Protein / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D035001:Host Factor 1 Protein; D012333:RNA, Messenger; D004722:Endoribonucleases; C022537:ribonuclease E