Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis.
Glieder
Anton
A
Institut für Biotechnologie, Technische Universität Graz, Petersgasse 12, 8010 Graz, Austria. glieder@glieder.com
Weis
Roland
R
Skranc
Wolfgang
W
Poechlauer
Peter
P
Dreveny
Ingrid
I
Majer
Sandra
S
Wubbolts
Marcel
M
Schwab
Helmut
H
Gruber
Karl
K
D000097:Acetonitriles / Q000737:chemistry; D002713:Chlorine / Q000737:chemistry; D006031:Glycosylation; D006836:Hydro-Lyases / Q000138:chemical synthesis*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009570:Nitriles / Q000138:chemical synthesis; D010843:Pichia; D012441:Saccharomyces cerevisiae
D000097:Acetonitriles; D009570:Nitriles; C430593:cyanohydrin; D002713:Chlorine; C045516:mandelonitrile; D006836:Hydro-Lyases; C048831:nitrile hydratase