title: Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with multisubstrate analogue inhibitor with 2,6-diaminopurine aglycone.
abstract: The crystal structure at 2.05 A resolution of calf spleen PNP complexed with stoichiometric concentration of acyclic nucleoside phosphonate inhibitor, 2,6-diamino-(S)-9-[2-(phosphonomethoxy)propyl]purine, in a new space group P2(1)2(1)2(1) which contains two full trimers in the asymmetric crystal unit is described.
authors.affiliation: Institut für Chemie-Kristallographie, Freie Universität Berlin, Berlin, Germany.
authors.forename: Gertraud
authors.initials: G
authors.lastname: Koellner
authors.forename: Albrecht
authors.initials: A
authors.lastname: Stroh
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Raszewski
authors.forename: Antonin
authors.initials: A
authors.lastname: Holý
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bzowska
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray / Q000379:methods; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology*; D013154:Spleen / Q000201:enzymology
chemical_list: C479442:2,6-diaminopurine aglycone; D004791:Enzyme Inhibitors; D046911:Macromolecular Substances; D011687:Purines; D004317:Doxorubicin; D011683:Purine-Nucleoside Phosphorylase