Detection of unusual carotenoid esters in fresh mango (Mangifera indica L. cv. 'Kent').
The carotenoid pattern of mango cv. 'Kent' was investigated by LC-(APcI)MS analyses. In solvent extracts from the mesocarp an unusual carotenoid ester was identified as violaxanthin dibutyrate. For unequivocal identification of butyric acid by an independent method, total lipids were isolated by solvent extraction from the fruit flesh and analyzed by GC after saponification and subsequent methylation. Thus, evidence of butyric acid (1.6 area%) was provided. To the best of our knowledge, this is the first report on a xanthophyll dibutyrate in plants. Additionally, further carotenoid peaks were tentatively assigned to 9-cis-violaxanthin and neochrom or luteoxanthin, respectively, by their UV/vis and MS data of the saponified extracts.
Pott
Isabell
I
Institute for Agricultural Engineering in the Tropics and Subtropics, Hohenheim University, Garbenstrasse 9, D-70599 Stuttgart, Germany.
Breithaupt
Dietmar E
DE
Carle
Reinhold
R
D020148:Butyric Acid / Q000032:analysis / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D031022:Mangifera / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010860:Pigments, Biological; D010936:Plant Extracts / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D010860:Pigments, Biological; D010936:Plant Extracts; D024341:Xanthophylls; D019207:beta Carotene; D020148:Butyric Acid; D002338:Carotenoids; C005613:violaxanthin