title: A prokaryotic alternative oxidase present in the bacterium Novosphingobium aromaticivorans.
abstract: The alternative oxidase (AOX) is a terminal oxidase present in the respiratory chain of all plants as well as some yeasts and trypanosomes, but has not previously been found in a prokaryote. We have identified an AOX homologue in Novosphingobium aromaticivorans, the first AOX found in a prokaryote. We have cloned the gene for the N. aromaticivorans AOX and showed it to have a terminal oxidase activity when heterologously expressed in Escherichia coli. We have also shown that this novel AOX is expressed in N. aromaticivorans cells, and that its expression level is greatly influenced by the oxygen level and carbon source of the growth media.
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Roslagstullsbacken 15, Albanova University Center, SE-10691 Stockholm, Sweden.
authors.forename: Pål
authors.initials: P
authors.lastname: Stenmark
authors.forename: Pär
authors.initials: P
authors.lastname: Nordlund
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D003470:Culture Media; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D000099188:Alternative Oxidase