title: Evaluation of the anti-asthmatic property of Asystasia gangetica leaf extracts.
abstract: The leaf of Asystasia gangetica T. Adams (Acanthaceae) is used in many parts of Nigeria for the management of asthma. This study was aimed at investigating the anti-asthmatic property of hexane, ethylacetate, and methanol extracts of the leaves of Asystasia gangetica, obtained by successive sohxlet extraction. The results indicated that the extracts did not exhibit contractile or relaxant activity in isolated tissue preparations; however, they inhibited the contraction evoked by spasmogens; the IC(50) were calculated, where possible. The extracts relaxed histamine-precontracted tracheal strips in the following degree of potency-ethylacetate extract>hexane extract=methanol extract. The extracts also exhibited anti-inflammatory activity in the order of magnitude-methanol extract>hexane extract>ethylacetate extract. Acute toxicity test estimated an i.p. LD(50) of 2150 mg/kg in mice for methanol extract while phytochemical screening showed the presence of carbohydrates, proteins, alkaloids, tannins, steroidal aglycones, saponins, flavonoids, reducing sugars, and triterpenoids, with the methanol extract having the highest number of constituents. The study justified the use of the leaf of Asystasia gangetica in the management of asthma in Nigerian folk medicine.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Enugu State, Nsukka, Nigeria. peterakah@hotmail.com
authors.forename: P A
authors.initials: PA
authors.lastname: Akah
authors.forename: A C
authors.initials: AC
authors.lastname: Ezike
authors.forename: S V
authors.initials: SV
authors.lastname: Nwafor
authors.forename: C O
authors.initials: CO
authors.lastname: Okoli
authors.forename: N M
authors.initials: NM
authors.lastname: Enwerem
mesh_terms: D027901:Acanthaceae; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use* / Q000633:toxicity; D001249:Asthma / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy; D007928:Lethal Dose 50; D016488:Medicine, African Traditional; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology; D009549:Nigeria; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D018515:Plant Leaves; D051381:Rats; D023382:Toxicity Tests, Acute; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology
chemical_list: D018927:Anti-Asthmatic Agents; D010936:Plant Extracts