Inter-relationship between electrophoretic characteristics of pseudocereal and cereal proteins and their microscopic structure for possible substitution based on nutritional evaluation.
Amaranth, soybean and maize were screened for proteins and their nutritional value. Isopropanol-soluble protein and buffer-soluble protein fractions were extracted from seeds and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison of the identity and differences between investigated plants was carried out by the obtained SDS-PAGE electrophoretic patterns, and their microstructure was determined by scanning electron microscopy. Electrophoretic patterns of extracted proteins have shown that the main protein subunits were concentrated between 10 and 50 kDa. Variations were found in major fractions and minor bands as well as in the fine structure. The microstructure of pseudocereal and cereal protein fractions was inter-related with the results obtained by their electrophoretic separation. Pseudocereal amaranth can be used as a nutritive substitute of cereal maize in functional foods.
Gorinstein
Shela
S
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University-Hadassah Medical School, P.O.B. 12065, Jerusalem 91120, Israel. gorin@cc.huji.ac.il
Yamamoto
Kazutaka
K
Kobayashi
Shoichi
S
Taniguchi
Hajime
H
Pawelzik
Elke
E
Delgado-Licon
Efren
E
Shaoxian
Yue
Y
Hongliang
Sun
S
Ayala
Alma Leticia Martinez
AL
Trakhtenberg
Simon
S
D019840:2-Propanol / Q000737:chemistry; D027721:Amaranthus / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D004044:Dietary Proteins; D002523:Edible Grain / Q000737:chemistry* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005983:Glutens / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009753:Nutritive Value; D010940:Plant Proteins / Q000737:chemistry*; D055315:Prolamins; D051381:Rats; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry / Q000648:ultrastructure; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry / Q000648:ultrastructure
D004044:Dietary Proteins; D010940:Plant Proteins; D055315:Prolamins; D005983:Glutens; D019840:2-Propanol