title: Acylation-stimulating protein precursor proteins in adipose tissue in human obesity.
abstract: Recent reports have suggested a link between acylation-stimulating protein (ASP) and complement C3 with obesity, insulin resistance, coronary artery disease, and hyperlipidemia. Our aim was to examine the mRNA expression of C3 and other factors related to ASP production (such as factor B and adipsin) in adipose tissue. The influence of gender and obesity was examined in subcutaneous (SC) and omental (OM) tissues from 16 males and 16 females with body mass index (BMI) from 20 to 54 kg/m(2). The results demonstrate that factor B mRNA expression is higher in males than females in both SC and OM tissues. In female SC tissue, C3 and adipsin mRNA decrease with increasing BMI (r = 0.557, P =.025 and r = 0.717 P =.002, respectively), with no change in factor B. By contrast, in males there was a pronounced increase in C3, adipsin, and factor B in OM tissue with increasing BMI (r = 0.759 P =.001, r = 0.650 P =.006, and r = 0.568 P =.022, respectively). Of note, however, in both men and women there was a marked increase in the OM/SC ratio of C3 and adipsin with increasing BMI. These results suggest that in female SC adipose tissue, there is downregulation of factors related to ASP production in obesity, perhaps to limit further expansion of adipose tissue. In males, there is increased expression in OM tissue. In addition, relative OM/SC expression increases with obesity and these changes may contribute to the development of visceral adipose tissue.
authors.affiliation: Mike Rosenbloom Laboratory for Cardiovascular Research, McGill University Health Centre, Montreal, Quebec, Canada.
authors.forename: Zhunan
authors.initials: Z
authors.lastname: Xia
authors.forename: Katherine
authors.initials: K
authors.lastname: Cianflone
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000328:Adult; D001824:Body Constitution; D015992:Body Mass Index; D003176:Complement C3 / Q000235:genetics / Q000378:metabolism*; D011415:Complement Factor B / Q000235:genetics / Q000378:metabolism*; D011416:Complement Factor D; D005260:Female; D015870:Gene Expression; D006801:Humans; D016014:Linear Models; D008297:Male; D009765:Obesity / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors
chemical_list: D003176:Complement C3; D012333:RNA, Messenger; D012697:Serine Endopeptidases; C493488:CFD protein, human; D011416:Complement Factor D; D011415:Complement Factor B