title: DNA gains at 8q23.2: a potential early marker in head and neck carcinomas.
abstract: Gains or amplifications involving chromosome arm 8q are one of the most recurrent chromosomal alterations in head and neck tumors. To characterize previously reported gains, we performed fluorescence in situ hybridization (FISH) using the sequences BAC RP1179E1 and 8-centromere PMJ 128 as probes. Gains and/or amplifications were detected in all 19 cases evaluated by FISH. The FISH analysis, but not G-banding, revealed homogeneously staining region in three cases. We conclude that gains of one or more genes on chromosome arm 8q may be important for the early stages of head and neck carcinomas.
authors.affiliation: Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Luciana Caricati
authors.initials: LC
authors.lastname: da Silva Veiga
authors.forename: Nádia Aparecida
authors.initials: NA
authors.lastname: Bérgamo
authors.forename: Patrícia Pintor
authors.initials: PP
authors.lastname: dos Reis
authors.forename: Luis Paulo
authors.initials: LP
authors.lastname: Kowalski
authors.forename: Silvia Regina
authors.initials: SR
authors.lastname: Rogatto
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm