title: Modification of Ti6Al4V surfaces using collagen I, III, and fibronectin. I. Biochemical and morphological characteristics of the adsorbed matrix.
abstract: Studies in developmental and cell biology have established the fact that responses of cells are influenced to a large degree by morphology and composition of the extracellular matrix. Goal of this work is to use this basic principle to improve the biological acceptance of implants by modifying the surfaces with components of the extracellular matrix (ECM). Aiming at load-bearing applications in bone contact, in this study the modification of titanium surfaces with the collagen types I and III in combination with fibronectin was undertaken; fibrillogenesis, fibril morphology and adsorption of type I, III and I/III-cofibrils onto titanium were assessed. Increasing the collagen type III amount resulted in a decrease of fibril diameter, while no significant changes in adsorption could be detected. The amount of fibronectin bound to the heterotypic fibrils depended on fibrillogenesis parameters such as ionic strength or concentration of phosphate, and varied with the percentage of integrated type III collagen.
authors.affiliation: Institute of Materials Science, Dresden University of Technology, Dresden, Germany. Susanne.bierbaum@mailbax.tu-dresden.de
authors.forename: Susanne
authors.initials: S
authors.lastname: Bierbaum
authors.forename: René
authors.initials: R
authors.lastname: Beutner
authors.forename: Thomas
authors.initials: T
authors.lastname: Hanke
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Hartmut
authors.initials: H
authors.lastname: Worch
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry*; D024061:Collagen Type III / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D014025:Titanium / Q000737:chemistry
chemical_list: D000497:Alloys; D024042:Collagen Type I; D024061:Collagen Type III; D005353:Fibronectins; D000535:Aluminum; D014025:Titanium