[Component analysis of bone matrix via cell extraction].
OBJECTIVE
To investigate the preparation of bone acellular extra-cell matrix(AECM) and to analyze its component.


METHODS
With low-osmosis theory and method of cell extraction by detergent, bone acellular extra-cell matrix was prepared. We observed morphologic changes with HE, Mallory-Heidenhain rapid one-step dyeing and Alcian blue dyeing and examined fibronectin(FN) and laminin(LN) with immunohistochemistry.


RESULTS
Light microscope showed that the collagen fibers arranged regularly in AECM with blankness of bone lacunas by HE, Mallory-Heidenhain rapid one-step dyeing and that the region around bone lacunas was stained different degrees of blue-green by Alcian blue dyeing. The result of immunohistochemistry showed there are positive markers of FN and LN in ECM.


CONCLUSIONS
This method for preparation of bone acellular extra-cell matrix is effective, and it can keep natural structure of collagen fibers and maintain components of ECM, such as proteoglycan, FN and LN.
Wang
Hao
H
Department of Tissue Engineering, China Medical University, Shenyang, Liaoning, P. R. China 110001.
Bai
Shu-ling
SL
D001842:Bone and Bones / Q000737:chemistry*; D002469:Cell Separation; D003094:Collagen / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis*; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis*; D011509:Proteoglycans / Q000032:analysis; D023822:Tissue Engineering
D005353:Fibronectins; D007797:Laminin; D011509:Proteoglycans; D003094:Collagen