Overexpression of the FRO2 ferric chelate reductase confers tolerance to growth on low iron and uncovers posttranscriptional control.
The Arabidopsis FRO2 gene encodes the low-iron-inducible ferric chelate reductase responsible for reduction of iron at the root surface. Here, we report that FRO2 and IRT1, the major transporter responsible for high-affinity iron uptake from the soil, are coordinately regulated at both the transcriptional and posttranscriptional levels. FRO2 and IRT1 are induced together following the imposition of iron starvation and are coordinately repressed following iron resupply. Steady-state mRNA levels of FRO2 and IRT1 are also coordinately regulated by zinc and cadmium. Like IRT1, FRO2 mRNA is detected in the epidermal cells of roots, consistent with its proposed role in iron uptake from the soil. FRO2 mRNA is detected at high levels in the roots and shoots of 35S-FRO2 transgenic plants. However, ferric chelate reductase activity is only elevated in the 35S-FRO2 plants under conditions of iron deficiency, indicating that FRO2 is subject to posttranscriptional regulation, as shown previously for IRT1. Finally, the 35S-FRO2 plants grow better on low iron as compared with wild-type plants, supporting the idea that reduction of ferric iron to ferrous iron is the rate-limiting step in iron uptake.
Connolly
Erin L
EL
Department of Biological Sciences, Coker Life Sciences, University of South Carolina, Columbia, South Carolina 29208, USA. erinc@biol.sc.edu
Campbell
Nathan H
NH
Grotz
Natasha
N
Prichard
Charis L
CL
Guerinot
Mary Lou
ML
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D038181:FMN Reductase / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019441:Plant Epidermis / Q000201:enzymology / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology
D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; C099651:IRT1 protein, Arabidopsis; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D015032:Zinc