title: Frameshift mutation events in beta-glucosidases.
abstract: Compensated frameshift mutation is a modification of the reading frame of a gene that takes place by way of various molecular events. It appears to be a widespread event that is only observed when homologous amino acid and nucleodotide sequences are compared. To identify these mutation events, the sequence analysis rationale was based on the search for short regions that would have much lower degrees of conservation in protein, but not in DNA, in well-conserved beta-glucosidase families. We have restricted our study to a seed set of sequences of O-glycoside hydrolase families 1 and 3. We found compensated frameshift mutation in the family of 1 beta-glucosidases for the Erwinia herbicola, Cellulomonas fimi, and (non-cyanogenic) Trifolium repens gene sequences, and in the family of 3 beta-glucosidases for the Clostridium thermocellum and Clostridium stercorarium gene sequences. By computational treatment, the observed mutation events in the gene frameshifting sub-sequence have been neutralised. Each nucleotide insertion must be eliminated and each nucleotide deletion must be substituted by the symbol N (any nucleotide). When the frameshifting fragments of the amino acid sequences were substituted by the computationally neutralised subsequences, the beta-glucosidase alignments were improved. We also discuss the structural implications of the compensated frameshift mutations events.
authors.affiliation: Evolutionary Genomics Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Pl. Imperial Tàrraco, 1. E-43005, Catalonia, Tarragona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rojas
authors.forename: Santiago
authors.initials: S
authors.lastname: Garcia-Vallvé
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Montero
authors.forename: Lluís
authors.initials: L
authors.lastname: Arola
authors.forename: Antoni
authors.initials: A
authors.lastname: Romeu
mesh_terms: D000595:Amino Acid Sequence; D044602:Cellulases / Q000235:genetics*; D040143:Cellulomonas / Q000201:enzymology / Q000235:genetics; D003013:Clostridium / Q000201:enzymology / Q000235:genetics; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D004885:Erwinia / Q000201:enzymology / Q000235:genetics; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000379:methods; D017386:Sequence Homology, Amino Acid; D029921:Trifolium / Q000201:enzymology / Q000235:genetics
chemical_list: D044602:Cellulases