title: Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing.
abstract: Fluorescence resonance energy transfer (FRET) dye labeled cassettes and terminators with one or more donor dyes (fluorescein) and acceptor dye (rhodamine dyes) with benzofuran or tyrosine linker moieties were synthesized. These terminators were evaluated for their energy transfer and DNA sequencing potential using thermostable DNA polymerase.
authors.affiliation: Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA.
authors.forename: T
authors.initials: T
authors.lastname: Sudhakar Rao
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhang
authors.forename: Haiguang
authors.initials: H
authors.lastname: Xiao
authors.forename: Parke
authors.initials: P
authors.lastname: Flick
authors.forename: Shiv
authors.initials: S
authors.lastname: Kumar
authors.forename: Satyam
authors.initials: S
authors.lastname: Nampalli
mesh_terms: D001483:Base Sequence; D001572:Benzofurans; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D013728:Terminator Regions, Genetic; D014443:Tyrosine
chemical_list: D001572:Benzofurans; D014443:Tyrosine; D004247:DNA; C105430:benzofuran