title: Crystallization and preliminary X-ray diffraction study of hemoglobin D from the Aldabra giant tortoise, Geochelone gigantea.
abstract: Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 A resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta = 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).
authors.affiliation: Institute of Quantum Science, Nihon University, Funabashi 274-8501, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Kuwada
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Isamu
authors.initials: I
authors.lastname: Satoh
authors.forename: Koichi
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Shishikura
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D003627:Data Interpretation, Statistical; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000302:isolation & purification; D014426:Turtles
chemical_list: D006455:Hemoglobins, Abnormal; C032001:hemoglobin D