Total stepwise solid-phase peptide-oligonucleotide conjugate synthesis on macroporous polystyrene.
An efficient total stepwise solid-phase synthesis of oligonucleotide-peptide conjugates on a macroporous polystyrene is described. Extending our homoserine linker approach, we prepared a range of fluorescein-labelled conjugates containing one of two different peptides together with oligonucleotides containing 2'-deoxynucleoside or 2'-O-methylribonucleoside phosphodiesters, or gapmers containing 2'-deoxyphosphorothioate sequences flanked by 2'-O-methyl wings.
Stetsenko
Dmitry A
DA
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK. ds@mrc-lmb.cam.ac.uk
Malakhov
Andrey D
AD
Gait
Michael J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D019793:Fluorescein; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D020319:Oligodeoxyribonucleotides, Antisense / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D011137:Polystyrenes
D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense; D009842:Oligopeptides; D020135:Peptide Nucleic Acids; D011137:Polystyrenes; D019793:Fluorescein