Inflammatory responses induced in mice by lectin from Talisia esculenta seeds.
A novel lectin from Talisia esculenta seeds (TEL) has recently been purified and characterized. In this study we investigated the proinflammatory activity of TEL in mice using both the air-pouch and peritoneal cavity as well as paw oedema models. TEL (10-40 microg) induced significant neutrophil and mononuclear cell recruitment when injected into either mouse air-pouch or peritoneal cavity. The neutrophil accumulation into the air-pouch was dose- and time-dependent with a maximal response at 16 h, returning to control levels at 72 h whereas maximal mononuclear cell accumulation was observed at 24 h after TEL injection. The same profile of neutrophil accumulation was observed when this lectin was injected into mouse peritoneal cavity, although the maximal mononuclear cell recruitment was observed 48 h after TEL injection. Additionally, TEL (12.5-200 microg/paw) caused a dose-dependent mice paw, as evaluated at 4 h after the lectin injection. D-mannose, better than D-glucose, significantly inhibited TEL-induced neutrophil migration into the peritoneal cavity or air-pouch. D-galactose had no effect on TEL-induced neutrophil migration in either cavity studied. On the other hand, D-mannose slightly inhibited the TEL-induced paw oedema, whereas neither D-glucose nor D-galactose affected this phenomenon. In conclusion, our data show that TEL induces neutrophil and mononuclear cell accumulation by a mechanism related to their specific sugar-binding properties.
Freire
M G M
MG
Departamento de Bioqui;mica, Instituto de Biologia (IB), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zefferino Vaz, C.P. 6109, CEP 13083-970 Campinas, SP, Brazil. maria.freire@terra.com.br
Desouza
I A
IA
Silva
A C M
AC
Macedo
M L R
ML
Lima
M S
MS
Tamashiro
W M S C
WM
Antunes
E
E
Marangoni
S
S
D000704:Analysis of Variance; D000818:Animals; D002241:Carbohydrates / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004487:Edema / Q000139:chemically induced / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced* / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D009504:Neutrophils / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D037121:Plant Lectins / Q000276:immunology / Q000633:toxicity*; D029631:Sapindaceae / Q000737:chemistry; D012639:Seeds / Q000737:chemistry
D002241:Carbohydrates; D037121:Plant Lectins