title: Optimization of in situ hybridization protocols for detection of feline herpesvirus 1.
abstract: In situ hybridization (ISH) protocol including microwaving pre-treatment regimes was developed and compared with protease digestion as a pre-treatment regime for its effects on detecting feline herpesvirus 1 (FHV-1) in formalin fixed, paraffin embedded tissue. We found that optimum results were obtained using microwave pre-treatment. The results showed that the use of microwave irradiation would be recommended as a means of supplementing ISH methods, especially when using long-term formalin fixed, paraffin-embedded tissue.
authors.affiliation: USDA-ARS, ADRU, USA.
authors.forename: Okjin
authors.initials: O
authors.lastname: Kim
authors.forename: Seong Joon
authors.initials: SJ
authors.lastname: Yi
mesh_terms: D000818:Animals; D002415:Cats / Q000821:virology*; D015342:DNA Probes; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary
chemical_list: D015342:DNA Probes