Water-soluble cyclodextrin polymers for enhanced relative recovery of hydrophobic analytes during microdialysis sampling.
Microdialysis relative recovery (RR) enhancement using different water-soluble, epichlorohydrin-based cyclodextrin polymers (CD-EPS) was studied in vitro for different analytes, amitryptiline, carbamazepine, hydroquinone, ibuprofen, and 4-nitrophenol. When compared to the native CDs (alpha, beta, and gamma) on a per mole basis, the CD-EPS enhanced microdialysis RR was either statistically greater or the same. beta-CD-EPS was more highly retained than native beta-CD by a 20 000 Da molecular weight cutoff (MWCO) polycarbonate membrane, but showed no statistical difference for loss across a 100 000 Da MWCO polyethersulfone membrane (PES). When the same weight percent of beta-CD or beta-CD-EPS was included in the microdialysis perfusion fluid, the beta-CD-EPS produced a higher microdialysis RR than native beta-CD for all analytes across the PES membrane. However, enhancements for the PC membrane were statistically insignificant when beta-CD and beta-CD-EPS were compared on a per mole basis. These results suggest that CD-EPS may be used as effective enhancement agents during microdialysis sampling and for some membranes provide the additional advantage of being retained more than native CDs.
Ao
Xiaoping
X
Department of Chemistry, 130 Cogswell Laboratories, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180-3590, USA.
Stenken
Julie A
JA
D002482:Cellulose; D003505:Cyclodextrins; D004811:Epichlorohydrin; D008567:Membranes, Artificial; D017551:Microdialysis / Q000379:methods*; D014462:Ultrafiltration / Q000379:methods
D003505:Cyclodextrins; D008567:Membranes, Artificial; C041953:cyclodextrin polymer; D004811:Epichlorohydrin; D002482:Cellulose