title: Genomic distribution of three repetitive DNAs in cultivated hexaploid Diospyros spp. (D. kaki and D. virginiana) and their wild relatives.
abstract: To understand the genomic organization of Diospyros species with different ploidy levels, we cloned three different repetitive DNAs and compared their genomic distributions in ten Diospyros species, including hexaploid D. kaki and D. virginiana. Genomic Southern hybridization demonstrated that the EcoRV-repetitive DNA was present in tandem in the genomes of D. glandulosa (2n=2x=30), D. oleifera (2n=2x=30), D. lotus (2n=2x=30), D. virginiana (2n=6x=90) and D. kaki (2n=6x=90). All of these species except D. virginiana also contained the HincII-repetitive DNA in tandem. Fluorescent in situ hybridization showed that the EcoRV- and HincII-repetitive DNAs were predominantly located at the proximal or centromeric regions of chromosomes. The DraI-repetitive sequence cloned from D. ehretioides (2n=2x=30) was not found in the other Diospyros species tested. This suggests that D. ehretioides has a genomic organization different from that of the other Diospyros species. Speciation of hexaploid Diospyros species is also discussed with respect to the genomic distribution of the three repetitive DNAs cloned.
authors.affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. choi@kais.kyoto-u.ac.jp
authors.forename: Young A
authors.initials: YA
authors.lastname: Choi
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Tao
authors.forename: Keizo
authors.initials: K
authors.lastname: Yonemori
authors.forename: Akira
authors.initials: A
authors.lastname: Sugiura
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D029788:Diospyros / Q000235:genetics*; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011123:Polyploidy; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018744:DNA, Plant; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases