Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale.
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
Valdés
Rodolfo
R
Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Reyes
Biunayki
B
Alvarez
Tatiana
T
García
Janet
J
Montero
José A
JA
Figueroa
Alejandro
A
Gómez
Leonardo
L
Padilla
Sigifredo
S
Geada
Déborah
D
Abrahantes
Ma Carmen
MC
Dorta
Lamay
L
Fernández
Damián
D
Mendoza
Otto
O
Ramirez
Nadia
N
Rodriguez
Marylin
M
Pujol
Merardo
M
Borroto
Carlos
C
Brito
José
J
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D017325:Hepatitis B Vaccines / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000276:immunology*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D008024:Ligands; D010940:Plant Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic