A simplified cytomegalovirus pp65 antigenemia assay procedure.
A simplified cytomegalovirus (CMV) pp65 antigenemia assay using a one-step erythrocyte lysis, fixation and permeabilization process was compared with a standard protocol, the CMV CINAkit (Argene Biosoft) assay. The results were comparable, both quantitatively and qualitatively. The new method saves time. It also provides flexibility because the cell suspension can be stored so that test completion can be deferred if so desired.
Gratacap-Cavallier
B
B
Laboratoire de Virologie, Centre Hospitalier Universitaire, 38043 Grenoble cedex 09, France. bgratacap@chu-grenoble.fr
Bonadona
A
A
Berthier
R
R
Brambilla
E
E
Seigneurin
J M
JM
Lorimier
P
P
D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D010750:Phosphoproteins / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D013997:Time Factors; D014763:Viral Matrix Proteins / Q000097:blood*; D014766:Viremia / Q000175:diagnosis* / Q000821:virology
D010750:Phosphoproteins; D011933:Reagent Kits, Diagnostic; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa