Optimization of cultural conditions for thermostable beta-1,3-1,4-glucanase production by Bacillus subtilis ZJF-1A5.
The optimization of cultural conditions for Beta-glucanase production by Bacillus subtilis ZJF-1A5 was investigated in flask trials. Temperature had great effect on Beta-glucanase production which maximized at optimal temperature of 37 degrees C and decreased significantly when temperature was over 37 degrees C. Charge quantity affected Beta-glucanase production significantly. Adding oxygen vector N-dodecane or acetic ether benefited Beta-glucanase production, but it depended on the concentration and charge quantity. The results of fractional factorial design showed that age and size of inoculum and shaking speed were the key factors affecting Beta-glucanase production and the cultivation time span to reach the highest Beta-glucanase activity. The optimal cultural conditions for Beta-glucanase production obtained with CCD were as follows: inoculum age and size (16 h, 3.82%(v/v)), shaking speed 210 r/min, charge quantity of 30 mL in 250 mL flask and initial pH 7.0, cultured at 37 degrees C for 50 h. Repeated experimental results accorded with those predicted by a second-order polynomial model. The amount of Beta-glucanase, Alpha-amylase and neutral protease produced by B subtilis ZJF-1A5 was associated partially with cell growth. Those three enzymes' activities increased following the cell growth and increased significantly when cells entered the stationary phase.
He
Guo-qing
GQ
College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029 China; gqhe@zju.edu.cn
Tang
Xing-jun
XJ
Mukhtar
A M Ali
AM
Chen
Qi-he
QH
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D001709:Biotechnology; D003470:Culture Media; D044644:Endo-1,3(4)-beta-Glucanase / Q000096:biosynthesis*; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010100:Oxygen; D012044:Regression Analysis; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D044644:Endo-1,3(4)-beta-Glucanase; D010100:Oxygen