[Experimental studies on the poly-hydroxybutyrate membrane modified by gamma-radiation and mixed with calcium sulfate].
The objective of this study was to learn the property of poly-hydroxybutyrate membrane (PHBm) modified by gamma-radiation and mixture of calcium sulfate, and to explore the possibility of using modified PHBm for guided tissue regeneration (GTR). The PHB was treated by 5 KGy gamma-radiation and mixed with 1/10 calcium sulfate. The modified PHB membrane was prepared by solvent-casting techniques. The mechanical properties and molecular weight of the modified PHBm were tested. Degradability of the modified PHBm was analyzed in vitro in a buffer solution of KH2PO4-Na2HPO4. Biodegradability and biocompatibility of the modified PHBm were inspected 1, 2, 3 and 6 months after the embedding of the modified PHBm into dogs. The morphology was analyzed by scanning electron microscopy (SEM) and molecular weight was tested to evaluate the biodegradability of PHBm. Biocompatibility of the modified PHBm was observed through tissue response by light microscopy. The extension strength and the extension strain at fracture of the modified PHBm were 23.8 MPa and 1.0% respectively. The morphologic observation of the modified PHBm at different terms showed that the modified PHBm was biodegraded gradually in vitro and in vivo. The capsule surrounding the modified PHBm was mainly composed of fibrocytes and few lymphocytes. The longer the time elapsed, the thinner the capsule enveloping the modified PHBm grew. The modified PHBm possesses satisfactory mechanical properties and biocompatibility, and it is biodegradable in vitro and in vivo. The modified PHB membrane could be applied as GTR membrane.
Bao
Chongyun
C
West China College of Stomatology, Sichuan University, Biomaterial Research Center, Chengdu.
Tian
Weidong
W
Li
Ping
P
Li
Shengwei
S
Zhang
Xingdong
X
D000818:Animals; D002133:Calcium Sulfate / Q000138:chemical synthesis / Q000633:toxicity*; D004285:Dogs; D005720:Gamma Rays; D048091:Guided Tissue Regeneration; D006885:Hydroxybutyrates / Q000138:chemical synthesis / Q000528:radiation effects* / Q000633:toxicity; D008422:Materials Testing; D008567:Membranes, Artificial; D011091:Polyesters / Q000138:chemical synthesis / Q000528:radiation effects* / Q000633:toxicity; D000094122:Polyhydroxybutyrates
D002133:Calcium Sulfate; D006885:Hydroxybutyrates; D008567:Membranes, Artificial; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates