title: The oxygen dependency of cerebral oxidative metabolism in the newborn piglet studied with 31P NMRS and NIRS.
abstract: Mean cerebral saturation and changes in the oxidation state of the CuA centre of cytochrome oxidase were measured by near infra-red spectroscopy simultaneously with phosphorous metabolites and intracellular pH measured using 31P NMR spectroscopy during transient anoxia (inspired oxygen fraction = 0.0 for 105 seconds) in the newborn piglet brain. By collecting high quality 31P spectra every 10 seconds, it was possible to resolve the delay between the onset of anoxia and the fall in PCr and to show that the CuA centre of cytochrome oxidase reduced simultaneously with the fall in PCr. From these observations it is concluded that, at normoxia, oxygen tension at the mitochondrial level is substantially above a critical value at which oxidative metabolism becomes oxygen dependent.
authors.affiliation: Department of Radiology, Dartmouth College, Hanover, New Hampshire, USA.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Springett
authors.forename: Marzena
authors.initials: M
authors.lastname: Wylezinska
authors.forename: Ernest B
authors.initials: EB
authors.lastname: Cady
authors.forename: Veronica
authors.initials: V
authors.lastname: Hollis
authors.forename: Mark
authors.initials: M
authors.lastname: Cope
authors.forename: David T
authors.initials: DT
authors.lastname: Delpy
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013552:Swine
chemical_list: D010100:Oxygen