title: Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation.
abstract: We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.
authors.affiliation: Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. mtaga@cc.okayama-u.ac.jp
authors.forename: Masatoki
authors.initials: M
authors.lastname: Taga
authors.forename: Dai
authors.initials: D
authors.lastname: Tsuchiya
authors.forename: Minoru
authors.initials: M
authors.lastname: Murata
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D002453:Cell Cycle; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics; D006999:Hypocreales / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D009690:Nucleic Acid Conformation; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal