Lithium treatment induces a hypersensitive-like response in tobacco.
Treatment of tobacco ( Nicotiana tabacum L.) plants with lithium induces the formation of necrotic lesions and leaf curling as in the case of incompatible pathogen interactions. Further similarities at the molecular level include accumulation of ethylene and of salicylic and gentisic acids, and induced expression of pathogenesis-related PR-P, PR5 and PR1 genes. With the exception of PR1 induction, lithium produced the same effects in transgenic tobacco plants that do not accumulate salicylate because of overexpression of the bacterial hydroxylase gene nahG. On the other hand, inhibition of ethylene biosynthesis with aminoethoxyvinylglycine prevented lithium-induced cell death and PR5 expression. These results suggest that lithium triggers a hypersensitive-like response where ethylene signalling is essential.
Naranjo
Miguel A
MA
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C., Camino de Vera s/n, 46022 Valencia, Spain.
Romero
Carlos
C
Bellés
José M
JM
Montesinos
Consuelo
C
Vicente
Oscar
O
Serrano
Ramón
R
D017209:Apoptosis / Q000187:drug effects*; D005030:Ethylenes / Q000378:metabolism; D005841:Gentisates / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D005030:Ethylenes; D005841:Gentisates; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; C036216:ethylene; D008094:Lithium; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid