title: Batch and continuous cultures of Mannheimia succiniciproducens MBEL55E for the production of succinic acid from whey and corn steep liquor.
abstract: Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is able to produce a large amount of succinic acid in a medium containing glucose, peptone, and yeast extract. In order to reduce the cost of the medium, whey and corn steep liquor (CSL) were used as substrates for the production of succinic acid by M. succiniciproducens MBEL55E. Anaerobic batch cultures of M. succiniciproducens MBEL55E in a whey-based medium containing CSL resulted in the production of succinic acid with a yield of 71% and productivity of 1.18 g/l/h, which are similar to those obtained in a whey-based medium containing yeast extract (72% and 1.21 g/l/h). Anaerobic continuous culture of M. succiniciproducens MBEL55E in a whey-based medium containing CSL resulted in a succinic acid yield of 69% and a succinic acid productivity as high as 3.90 g/l/h. These results show that succinic acid can be produced efficiently and economically by M. succiniciproducens MBEL55E from whey and CSL.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, 305-701 Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: P C
authors.initials: PC
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Hong
authors.forename: H N
authors.initials: HN
authors.lastname: Chang
mesh_terms: D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D007785:Lactose / Q000378:metabolism*; D031121:Mannheimia / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D013045:Species Specificity; D019802:Succinic Acid / Q000302:isolation & purification / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D019802:Succinic Acid; D007785:Lactose