DNA gains at 8q23.2: a potential early marker in head and neck carcinomas.
Gains or amplifications involving chromosome arm 8q are one of the most recurrent chromosomal alterations in head and neck tumors. To characterize previously reported gains, we performed fluorescence in situ hybridization (FISH) using the sequences BAC RP1179E1 and 8-centromere PMJ 128 as probes. Gains and/or amplifications were detected in all 19 cases evaluated by FISH. The FISH analysis, but not G-banding, revealed homogeneously staining region in three cases. We conclude that gains of one or more genes on chromosome arm 8q may be important for the early stages of head and neck carcinomas.
da Silva Veiga
Luciana Caricati
LC
Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil.
Bérgamo
Nádia Aparecida
NA
dos Reis
Patrícia Pintor
PP
Kowalski
Luis Paulo
LP
Rogatto
Silvia Regina
SR
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm