Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p.
Urm1p is a ubiquitin-related protein that serves as a posttranslational modification of other proteins. Urm1p conjugation has been implicated in the budding process and in nutrient sensing. Here, we have identified the first in vivo target for the urmylation pathway as the antioxidant protein Ahp1p. The attachment of Urm1p to Ahp1p requires the E1 for the urmylation pathway, Uba4p. Loss of the urmylation pathway components results in sensitivity to a thiol-specific oxidant, as does loss of Ahp1p, implying that urmylation has a role in an oxidative-stress response. Moreover, treatment of cells with thiol-specific oxidants affects the abundance of Ahp1p-Urm1p conjugates. These results suggest that the conjugation of Urm1p to Ahp1p could regulate the function of Ahp1p in antioxidant stress response in Saccharomyces cerevisiae.
Goehring
April S
AS
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229, USA.
Rivers
David M
DM
Sprague
George F
GF
D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005979:Glutathione Peroxidase; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000502:physiology; D010544:Peroxidases / Q000235:genetics / Q000502:physiology*; D054464:Peroxiredoxins; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000502:physiology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000502:physiology; D011328:Prions / Q000235:genetics / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D020123:Sirolimus / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D005979:Glutathione Peroxidase; D010544:Peroxidases; D054464:Peroxiredoxins; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D011328:Prions; D011506:Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D020123:Sirolimus; D014157:Transcription Factors; C071664:GLN3 protein, S cerevisiae; C406283:UBA4 protein, S cerevisiae; C406282:URM1 protein, S cerevisiae; C067020:URE2 protein, S cerevisiae; C083324:TOR1 protein, S cerevisiae