title: Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium.
abstract: The effects of acute exposure to cadmium (Cd) on the blood antioxidant defense system, lipid peroxide concentration and hematological parameters, as well as the possible protective role of vitamin E were studied. Male Wistar albino rats (3 months old) were treated with cadmium (0.4 mg Cd/kg b.m., i.p., 24 h before the experiment) or with vitamin E + Cd (20 IU Vit E/kg b.m., i.m., 48 h + 0.4 mg Cd/kg b.m., i.p., 24 h before the experiment). The hematological parameters were assessed: red blood cell counts, hematocrit value and hemoglobin concentration were significantly decreased in the blood of Cd-treated rats. Intoxication with cadmium was also followed by significantly increased lipid peroxide concentrations. We also observed increased activity of antioxidant defense enzymes: copper zinc containing superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase as well as concentrations of non-enzymatic components of antioxidant defense system: reduced glutathione, vitamin C and vitamin E. Pretreatment with vitamin E exhibited a protective role on the toxic effects of cadmium on the hematological values, lipid peroxide concentration as well as on enzymatic and non-enzymatic components of antioxidant defense system.
authors.affiliation: Institute of Biology, Faculty of Sciences, University of Kragujevac, Radoja Domanovića 12, P.O. Box 60, 34000 Kragujevac, Serbia, Yugoslavia. branka@knez.uis.kg.ac.yu
authors.forename: B I
authors.initials: BI
authors.lastname: Ognjanović
authors.forename: S Z
authors.initials: SZ
authors.lastname: Pavlović
authors.forename: S D
authors.initials: SD
authors.lastname: Maletić
authors.forename: R V
authors.initials: RV
authors.lastname: Zikić
authors.forename: A S
authors.initials: AS
authors.lastname: Stajn
authors.forename: R M
authors.initials: RM
authors.lastname: Radojicić
authors.forename: Z S
authors.initials: ZS
authors.lastname: Saicić
authors.forename: V M
authors.initials: VM
authors.lastname: Petrović
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D002104:Cadmium / Q000633:toxicity; D002105:Cadmium Poisoning / Q000097:blood / Q000517:prevention & control*; D002374:Catalase / Q000097:blood / Q000187:drug effects; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D005978:Glutathione / Q000097:blood / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000097:blood / Q000187:drug effects; D005980:Glutathione Reductase / Q000097:blood / Q000187:drug effects; D005982:Glutathione Transferase / Q000097:blood / Q000187:drug effects; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D006454:Hemoglobins; D008054:Lipid Peroxides; D002104:Cadmium; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid