title: Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment.
abstract: Tissue engineering aims at resolving problems such as donor shortage and immune rejection faced by transplantation. Scaffolds (artificial extracellular matrices) have critical roles in tissue engineering. Recently, we developed nano-fibrous poly(L-lactic acid) scaffolds under the hypothesis that synthetic nano-fibrous scaffolding, mimicking the structure of natural collagen fibers, could create a more favorable microenvironment for cells. This is the first report that the nano-fibrous architecture built in three-dimensional scaffolds improved the features of protein adsorption, which mediates cell interactions with scaffolds. Scaffolds with nano-fibrous pore walls adsorbed four times more serum proteins than scaffolds with solid pore walls. More interestingly, the nano-fibrous architecture selectively enhanced protein adsorption including fibronectin and vitronectin, even though both scaffolds were made from the same poly(L-lactic acid) material. Furthermore, nano-fibrous scaffolds also allowed >1.7 times of osteoblastic cell attachment than scaffolds with solid pore walls. These results demonstrate that the biomimetic nano-fibrous architecture serves as superior scaffolding for tissue engineering.
authors.affiliation: Department of Biologic and Materials Sciences, 1011 North University Avenue, Room 2211, The University of Michigan, Ann Arbor, Michigan 48109-1078, USA.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Woo
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Chen
authors.forename: Peter X
authors.initials: PX
authors.lastname: Ma
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid; D010006:Osteoblasts / Q000502:physiology; D011108:Polymers; D044967:Serum / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D011108:Polymers; D019344:Lactic Acid