Rice C2-domain proteins are induced and translocated to the plasma membrane in response to a fungal elicitor.
Hundreds of proteins involved in signaling pathways contain a Ca(2+)-dependent membrane-binding motif called the C2-domain. However, no small C2-domain proteins consisting of a single C2-domain have been reported in animal cells. We have isolated two cDNA clones, OsERG1a and OsERG1b, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library. The clones are believed to have originated from a single gene by alternative splicing. Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea or by Ca(2+) ions. The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca(2+)-dependent manner and is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Ca(2+) ionophore. These results suggest that OsERG1 proteins containing a single C2-domain are involved in plant defense signaling systems.
Kim
Cha Young
CY
Division of Applied Life Science, Gyeongsang National University, Jinju, Korea.
Koo
Yoon Duck
YD
Jin
Jing Bo
JB
Moon
Byeong Cheol
BC
Kang
Chang Ho
CH
Kim
Sun Tae
ST
Park
Byung Ouk
BO
Lee
So Young
SY
Kim
Man Lyang
ML
Hwang
Inhwan
I
Kang
Kyu Young
KY
Bahk
Jeong Dong
JD
Lee
Sang Yeol
SY
Cho
Moo Je
MJ
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D020013:Calcium Signaling / Q000502:physiology; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D020082:Magnaporthe / Q000472:pathogenicity* / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport
D008565:Membrane Proteins; D010743:Phospholipids; D010940:Plant Proteins