title: Antisense knockdown of PKC-alpha using LNA-oligos.
abstract: Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase C-alpha (PKC-alpha) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos.
authors.affiliation: Cureon A/S, Copenhagen, Denmark. bh@cureon.com
authors.forename: Jens Bo
authors.initials: JB
authors.lastname: Hansen
authors.forename: Majken
authors.initials: M
authors.lastname: Westergaard
authors.forename: Charlotte Albaek
authors.initials: CA
authors.lastname: Thrue
authors.forename: Birgit
authors.initials: B
authors.lastname: Giwercman
authors.forename: Henrik
authors.initials: H
authors.lastname: Oerum
mesh_terms: D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011493:Protein Kinase C / Q000235:genetics*; D051571:Protein Kinase C-alpha; D013873:Thionucleotides; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; C477371:locked nucleic acid; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha