title: Assessment of in vitro platelet activation by Advia 120 platelet parameters.
abstract: The Advia 120 Hematology System provides new platelet parameters that have been proposed as useful markers of platelet activation. The aim of this study was to investigate platelet parameter variations after adenosine diphosphate (ADP) activation of platelet-rich plasma (PRP) in vitro, with particular interest in the mean platelet component (MPC), which was compared with two well-known degranulation antigens. Changes in platelet parameters that were induced by the activation of PRP with different concentrations of ADP were examined first. The time course of parameter values up to 60 minutes after maximal ADP activation and the relationships between the MPC and P-selectin and granulophysin expression as determined by flow cytometry were then investigated. After 10 minutes of ADP stimulation, the MPC presented a dose-dependent increase. At the maximal ADP concentration, the initial increase of the MPC was followed by a progressive decrease, leading the MPC to become significantly lower with respect to the baseline after 60 minutes of incubation. Significant variations in other parameters are also described. Finally, a negative correlation was found between the MPC change with respect to time 0 and both P-selectin and granulophysin expression. The present study suggests that platelet parameter variation, particular in the MPC, may be used to assess platelet activation in PRPs stimulated by ADP.
authors.affiliation: Department of Clinical Pathology, Chioggia Hospital, Venezia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Giacomini
authors.forename: P
authors.initials: P
authors.lastname: Legovini
authors.forename: F
authors.initials: F
authors.lastname: Antico
authors.forename: S
authors.initials: S
authors.lastname: Valverde
authors.forename: M M
authors.initials: MM
authors.lastname: Salvadego
authors.forename: F
authors.initials: F
authors.lastname: Manoni
authors.forename: G
authors.initials: G
authors.lastname: Gessoni
mesh_terms: D000244:Adenosine Diphosphate; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001793:Blood Preservation; D006801:Humans; D007700:Kinetics; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010979:Platelet Function Tests / Q000295:instrumentation*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D000244:Adenosine Diphosphate