Influence of different culture solutions on osteoblastic differentiation in cord blood and bone marrow derived progenitor cells.
Mesenchymal progenitor cells derived from cord blood (unrestringated somatic stem cells, USSC) and bone marrow (mesenchymal stem cells, MSC) are able to differentiate under defined culture conditions into at least bone, cartilage, adipose and muscle cells in vitro. The culture media and other in vitro conditions influence the osteogenic differentiation potency of both cell types. To increase and expand the number of osteoblasts in vitro an optimization of culture conditions is required. The aim of this study was to evaluate different culture media toward their osteogenic promoting capacity on human USSCs and MSCs in vitro. Immunohistochemical stainings against osteonectin (ON), osteopontin (OP) served as markers for an osteoblastic differentiation. Cellular morphology was analysed by light microscopy technique. We found significant differences between bone marrow and cord blood derived stem cells towards an osteoblastic differentiation. Considering the number of osteoblasts MesenCult seems to have advantages in bone marrow progenitor cells, whereas low glucose DMEM and HAMS-F12 promoted an osteoblastic differentiation in cord blood derived cells more than other tested media.
Jäger
M
M
Department of Orthopedics, Heinrich-Heine University Hospital Duesseldorf. Jaeger@med.uni-duesseldorf.de
Wild
A
A
Lensing-Höhn
S
S
Krauspe
R
R
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000145:classification / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*
D003470:Culture Media; D005947:Glucose