title: Molecular determinants of proton modulation of glycine receptors.
abstract: Extracellular pH regulates glycine receptors through an unknown mechanism. Here we demonstrate that acidic pH remarkably inhibited glycine-activated whole-cell currents in recombinant glycine alpha1 and alpha1beta receptors transiently expressed in human embryonic kidney 293 cells. The proton effect was voltage-independent and pharmacologically competed with glycine receptor agonist glycine and antagonist strychnine. Using site-directed mutagenesis, we have identified an N-terminal domain that is essential for proton-induced inhibition of glycine current. In alpha1 homomers, removal of the hydroxyl group by mutation of residue Thr-112 to Ala or Phe abolished inhibition of glycine currents by acidification. In contrast, mutation of Thr-112 to another hydroxylated residue (Tyr) produced receptors that retained partial proton sensitivity. In alpha1beta heteromers, a single mutation of the beta subunit T135A, which is homologous to alpha1 Thr-112, reduced proton sensitivity, whereas the double mutation alpha1(T112A)beta(T135A) almost completely eliminated the proton sensitivity. In addition, the mutation alpha1 H109A greatly reduced sensitivity to protons in homomeric alpha1 receptors. The results demonstrate that extracellular pH can regulate the function of glycine alpha1 and alpha1beta receptors. An extracellular domain consisting of Thr-112 and His-109 at the alpha1 subunit and Thr-135 at the beta subunit plays a critical role in determining proton modulation of glycine receptor function.
authors.affiliation: Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA.
authors.forename: Zhenglan
authors.initials: Z
authors.lastname: Chen
authors.forename: Glenn H
authors.initials: GH
authors.lastname: Dillon
authors.forename: Renqi
authors.initials: R
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry; D002460:Cell Line; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D005998:Glycine / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011522:Protons; D011963:Receptors, GABA-A / Q000737:chemistry; D018009:Receptors, Glycine / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013331:Strychnine / Q000494:pharmacology; D013912:Threonine / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: C428944:AChBP protein, Lymnaea; D002352:Carrier Proteins; C114561:GLRB protein, human; D011522:Protons; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D013912:Threonine; D014443:Tyrosine; D013331:Strychnine; D005998:Glycine