[Isolation and culture of multipotent mesenchymal stem cells from porcine bone marrow].
OBJECTIVE
To establish a method for the isolation of porcine mesenchymal stem cells (MSCs) from bone marrow and to demonstrate their differentiation ex vivo into various mesenchymal tissue cells.


METHODS
MSCs were isolated from bone marrow and purified by centrifuge and in vitro. The proliferation and growth characteristics were observed in primary and passage culture. Cell cycle was analyzed by measuring DNA content with FAC-Scan flow cytometer and cell multipotent was identified with specific staining.


RESULTS
The adherent, fibroblast-like cells were confluent in single layer after plating for 12-14 days. The cultured MSCs in vitro differentiated into osteoblasts. The cell cycle analysis showed that 80% of MSCs were in G0/G1 phase.


CONCLUSIONS
Porcine MSCs can be isolated from postnatal bone marrow through their adherent ability. Porcine MSCs may be introduced as a valuable model system to study the mesenchymal lineages for basic research and tissue engineering.
Fang
Li-jun
LJ
Department of Stomatology, the Second Hospital of China Medical University, Shenyang 110003, Liaoning, China.
Fu
Xiao-bing
XB
Sun
Tong-zhu
TZ
Li
Jian-fu
JF
Chen
Biao
B
Wang
Yu-xin
YX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering