title: Quantitative analysis of testicular interstitial fibrosis after vasectomy in humans.
abstract: OBJECTIVE
  Germ cell differentiation, DNA synthesis, and apoptosis can be evaluated quantitatively. On the other hand, an interstitial lesion is difficult to examine. We have focused on the quantitative analysis of testicular interstitial fibrosis after vasectomy.
  
  
  METHODS
  Forty testicular biopsy specimens from twenty consecutive men were obtained at vasovasostomy. Johnsen's mean score was calculated from testicular biopsy specimens. Percent of interstitial fibrosis was determined quantitatively by the NIH-Image after Masson-trichrome staining.
  
  
  RESULTS
  A significant increase in interstitial fibrosis was observed along with the obstructive interval (p < 0.001). Johnsen's mean score count did not associate with the obstructive interval.
  
  
  CONCLUSIONS
  Interstitial lesions of testicular physiology and pathophysiology can be evaluated using the NIH-Image. Interstitial fibrosis, but not the intraseminiferous status, reflects the irreversible damage of vasectomized testes.
authors.affiliation: Department of Urology and Hemodialysis Center, Onoda City Hospital, Onoda, Yamaguchi, Japan. sirakkkay@rapid.ocn.ne.jp
authors.forename: K
authors.initials: K
authors.lastname: Shiraishi
authors.forename: H
authors.initials: H
authors.lastname: Takihara
authors.forename: K
authors.initials: K
authors.lastname: Naito
mesh_terms: D000328:Adult; D001706:Biopsy; D005355:Fibrosis / Q000000981:diagnostic imaging / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D013091:Spermatogenesis; D013194:Staining and Labeling; D013737:Testis / Q000000981:diagnostic imaging / Q000473:pathology*; D014463:Ultrasonography; D014659:Vasectomy; D014669:Vasovasostomy