sup-9, sup-10, and unc-93 may encode components of a two-pore K+ channel that coordinates muscle contraction in Caenorhabditis elegans.
Genetic studies of sup-9, unc-93, and sup-10 strongly suggest that these genes encode components of a multi-subunit protein complex that coordinates muscle contraction in Caenorhabditis elegans. We cloned sup-9 and sup-10 and found that they encode a two-pore K+ channel and a novel transmembrane protein, respectively. We also found that UNC-93 and SUP-10 colocalize with SUP-9 within muscle cells, and that UNC-93 is a member of a novel multigene family that is conserved among C. elegans, Drosophila, and humans. Our results indicate that SUP-9 and perhaps other two-pore K+ channels function as multiprotein complexes, and that UNC-93 and SUP-10 likely define new classes of ion channel regulatory proteins.
de la Cruz
Ignacio Perez
IP
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Department of Biology, Cambridge, Massachusetts 02139, USA.
Levin
Joshua Z
JZ
Cummins
Claudia
C
Anderson
Philip
P
Horvitz
H Robert
HR
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005091:Exons; D018755:GABA Agonists / Q000494:pharmacology; D020690:GTPase-Activating Proteins / Q000235:genetics; D049452:Green Fluorescent Proteins; D007438:Introns; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D009118:Muscimol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009124:Muscle Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D021122:Protein Subunits / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
C084219:BEM2 protein, S cerevisiae; D029742:Caenorhabditis elegans Proteins; D018755:GABA Agonists; D020690:GTPase-Activating Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; C478296:Sup-10 protein, C elegans; C478295:Sup-9 protein, C elegans; C073904:unc-93 protein, C elegans; D049452:Green Fluorescent Proteins; C420132:potassium channel subfamily K member 3; D009118:Muscimol