title: New reagent for protein-DNA contacts footprinting.
abstract: We have found, that the reaction of o-bromobenzoic acid with Cu2+ ions can be used as a source of activated oxygen species capable of cleaving DNA. Possibility to apply this reaction for footprinting the nucleosome core in the reconstituted chromatin was demonstrated.
authors.affiliation: Laboratory of Nucleic Acids Biochemistry, Institute of Bioorganic Chemistry, Novosibirsk, Russia. o.koval@niboch.nsc.ru
authors.forename: O A
authors.initials: OA
authors.lastname: Koval
authors.forename: S B
authors.initials: SB
authors.lastname: Oleinikova
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaya
authors.forename: V V
authors.initials: VV
authors.lastname: Litvak
authors.forename: V V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting / Q000379:methods*; D006868:Hydrolysis; D007202:Indicators and Reagents; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D009707:Nucleosomes; D011506:Proteins; D004247:DNA