title: [Biocompatibility of poly-l-lysine-modified silica nanoparticles].
abstract: OBJECTIVE
  Poly-l-lysine-modified silica nanoparticle(PMS-NP) was a novel non-viral vector for gene delivery. The current study was designed to evaluate the biocompatibility of PMS-NP for its further utilization in vivo.
  
  
  METHODS
  Cell transfection and flow cytometry were used to elucidate the delivery efficiency of plasmid DNA and antisense ODN mediated by PMS-NP in the presence of serum-containing medium. Subsequently, the biocompatibility of PMS-NP in vivo was evaluated using filtration assay of plasma proteins and erythrocyte aggregation assay.
  
  
  RESULTS
  The abilities of PMS-NP to deliver plasmid DNA and antisense ODN in vitro clearly decreased in the presence of serum-containing medium. PMS-NP/DNA(ODN)complexes bound plasma proteins and triggered erythrocyte aggregation.
  
  
  CONCLUSIONS
  PMS-NP might interact with plasma proteins, resulting in decreased transfection efficiency in vitro. And filtration assay of plasma proteins and the erythrocyte aggregation assay demonstrated that the interaction of PMS-NP with plasma proteins and erythrocytes might play a negative role in gene transfection efficiency in vivo. And its biocompatibility needs to be further improved.
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan, 410078, PR China.
authors.forename: Shi-Guo
authors.initials: SG
authors.lastname: Zhu
authors.forename: Kai
authors.initials: K
authors.lastname: Gan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Shou-Rong
authors.initials: SR
authors.lastname: Shen
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Xiang
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Li
authors.forename: Shong-Qing
authors.initials: SQ
authors.lastname: Fan
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Lu
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Zeng
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Li
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage*; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage; D010957:Plasmids; D011107:Polylysine; D011485:Protein Binding; D012822:Silicon Dioxide / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods*
chemical_list: D001798:Blood Proteins; D016895:Culture Media, Serum-Free; D020319:Oligodeoxyribonucleotides, Antisense; D011107:Polylysine; D012822:Silicon Dioxide