Construction of a homologous selectable marker gene for Lentinula edodes transformation.
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
Irie
Toshikazu
T
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Sato
Toshitsugu
T
Saito
Kumiko
K
Honda
Yoichi
Y
Watanabe
Takashi
T
Kuwahara
Masaaki
M
Enei
Hitoshi
H
D015139:Blotting, Southern; D002261:Carboxin / Q000494:pharmacology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005822:Genetic Vectors; D007506:Iron-Sulfur Proteins / Q000235:genetics; D017354:Point Mutation; D021122:Protein Subunits; D020942:Shiitake Mushrooms / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
D005819:Genetic Markers; D007506:Iron-Sulfur Proteins; D021122:Protein Subunits; D002261:Carboxin; D004262:DNA Restriction Enzymes