title: sup-9, sup-10, and unc-93 may encode components of a two-pore K+ channel that coordinates muscle contraction in Caenorhabditis elegans.
abstract: Genetic studies of sup-9, unc-93, and sup-10 strongly suggest that these genes encode components of a multi-subunit protein complex that coordinates muscle contraction in Caenorhabditis elegans. We cloned sup-9 and sup-10 and found that they encode a two-pore K+ channel and a novel transmembrane protein, respectively. We also found that UNC-93 and SUP-10 colocalize with SUP-9 within muscle cells, and that UNC-93 is a member of a novel multigene family that is conserved among C. elegans, Drosophila, and humans. Our results indicate that SUP-9 and perhaps other two-pore K+ channels function as multiprotein complexes, and that UNC-93 and SUP-10 likely define new classes of ion channel regulatory proteins.
authors.affiliation: Howard Hughes Medical Institute, Massachusetts Institute of Technology, Department of Biology, Cambridge, Massachusetts 02139, USA.
authors.forename: Ignacio Perez
authors.initials: IP
authors.lastname: de la Cruz
authors.forename: Joshua Z
authors.initials: JZ
authors.lastname: Levin
authors.forename: Claudia
authors.initials: C
authors.lastname: Cummins
authors.forename: Philip
authors.initials: P
authors.lastname: Anderson
authors.forename: H Robert
authors.initials: HR
authors.lastname: Horvitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005091:Exons; D018755:GABA Agonists / Q000494:pharmacology; D020690:GTPase-Activating Proteins / Q000235:genetics; D049452:Green Fluorescent Proteins; D007438:Introns; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D009118:Muscimol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009124:Muscle Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D021122:Protein Subunits / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: C084219:BEM2 protein, S cerevisiae; D029742:Caenorhabditis elegans Proteins; D018755:GABA Agonists; D020690:GTPase-Activating Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; C478296:Sup-10 protein, C elegans; C478295:Sup-9 protein, C elegans; C073904:unc-93 protein, C elegans; D049452:Green Fluorescent Proteins; C420132:potassium channel subfamily K member 3; D009118:Muscimol