Differential expression of homing receptor CD103 on lamina propria lymphocytes and association of CD103 with epithelial adhesion molecules in inflammatory bowel disease.
Intraepithelial lymphocytes play an important role in mucosal immunology, and are involved in the pathogenesis of inflammatory bowel disease. We studied expression of CD103 on mucosal lymphocytes with epithelial adhesion molecules in patients with inflammatory bowel disease. Surgical specimens of human colon were obtained from 12 patients with ulcerative colitis, 12 patients with Crohn's disease, and 5 controls. Frozen sections were cut and expression of CD103 on lymphocytes, E-cadherin, CD44V3, and CD44v6 on intestinal epithelium was studied. Frequency of CD103-positive intraepithelial lymphocytes did not differ among controls, patients with ulcerative colitis, and patients with Crohn's disease. The frequency of CD103-positive lamina propria lymphocytes was significantly higher in patients with Crohn's disease than in controls and patients with ulcerative colitis. The frequency of CD103-positive intraepithelial lymphocytes was significantly correlated with that of lamina propria lymphocytes in patients with ulcerative colitis. The frequency of CD103-positive intraepithelial lymphocytes was significantly correlated with epithelial E-cadherin expression but that of lamina propria lymphocytes was not. Differential up-regulation of CD103 expression on lamina propria lymphocytes in Crohn's disease may indicate differential humoral or cellular regulation in inducing CD103 molecules on lymphocytes in patients with this disease.
Oshitani
Nobuhide
N
Department of Gastroenterology, Osaka City University Medical School, Abeno-ku, Osaka 545-8585, Japan. nobu@med.osaka-cu.ac.jp
Watanabe
Kenji
K
Maeda
Kiyoshi
K
Fujiwara
Yasuhiro
Y
Higuchi
Kazuhide
K
Matsumoto
Takayuki
T
Arakawa
Tetsuo
T
D015703:Antigens, CD / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000378:metabolism* / Q000473:pathology; D039001:Integrin alpha Chains / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male
D015703:Antigens, CD; D015820:Cadherins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039001:Integrin alpha Chains; C091367:alpha E integrins