Novel biotin phosphoramidites with super-long tethering arms.
A number of novel biotin phosphoramidites, possessing exceptionally long and uncharged tethering arms, were synthesized from methoxyoxalamido (MOX) and succinimido (SUC) precursors. Included among these monomers is a uridine derivative with the biotin moiety attached through the 2'-position. Some of these phosphoramidites were used to make 5'-biotinylated primers, which were applied in direct sequencing of genomic DNA and capture of Sanger fragment pools.
Morocho
A M
AM
Fidelity Systems, Inc., Gaithersburg, Maryland 20879, USA.
Karamyshev
V N
VN
Shcherbinina
O V
OV
Malykh
A G
AG
Polushin
N N
NN
D000577:Amides / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D001710:Biotin; D019921:Biotinylation; D004247:DNA / Q000737:chemistry; D007202:Indicators and Reagents; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000138:chemical synthesis / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000577:Amides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010756:Phosphoric Acids; D001710:Biotin; D004247:DNA; C011067:phosphoramidic acid