title: Crystallization of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1.
abstract: The terminal oxygenase component of the biphenyl dioxygenase (BphA1A2 complex) was over-expressed with a novel over expression system in recombinant Rhodococcus strain and purified. The purified enzyme has been crystallized by the hanging drop vapor diffusion method and subjected to X-ray diffraction analysis. The crystals belong to the tetragonal system in the space group P4(1)2(1)2 or P4(3)2(1)2 and diffract to better than 2.2A resolution.
authors.affiliation: Division of Protein Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Venugopalan
authors.initials: V
authors.lastname: Nagarajan
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Sakurai
authors.forename: Miho
authors.initials: M
authors.lastname: Kubota
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nonaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagumo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Takeda
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nishizaki
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Mitsui
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D003627:Data Interpretation, Statistical; D015971:Gene Expression Regulation, Enzymologic; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics
chemical_list: D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D010105:Oxygenases; C074161:biphenyl-2,3-dioxygenase