Influence of modifying agents on enzyme inactivation studies. An analysis using a series mechanism and a form of the Hill-type equations.
A series deactivation model is utilized to theoretically examine the influence of different modifying agents on enzyme deactivation kinetics. A form of the Hill-type equation is used to describe the effect of the modifying agents on the model parameters. Modification-induced inactivation equations are presented for the acetylation and succinylation of E. Coli asparaginase, for the site-specific reagent and substrate modification of flavocytochrome b(2) from Baker's yeast, and for the guanidinium chloride inactivation of cathepsin D. The analysis of more data for these and other enzymes would help further substantiate the technique presented and enhance the applicability of the model.
Sadana
A
A
Chemical Engineering Department, University of Mississippi, University, MS 38677-9740, USA.
Henley
J P
JP