[Killer Ig-like receptor gene content diversity and haplotype analysis in Chinese Han population in Shanghai].
OBJECTIVE
To detect the diversity of killer Ig-like receptor(KIR) gene content and the combination of haplotypes in Chinese Han population in Shanghai area.


METHODS
DNA samples from 87 randomly unrelated healthy individuals in Shanghai Han population were genotyped with SSP/PCR method.


RESULTS
(1) Frequencies of KIR genes: All of 18 known KIRs genes, such as 2DL1-5, 2DS1-5, 3DL1-3, 3DS1, KIR1D and the pseudogenes X, Xv and Z(KIR2DP1) were observed in Shanghai Hans. All individuals contain 3DL3, 2DL4, 3DL2 and 3DL1; the most common genes were 2DL3, Z, 2DL1 and X; the following were 2DS4, 1D, 2DL5, 2DS1, 3DS1 and 2DS5; the next were 2DS2, 2DL2, 2DS3 and Xv. (2) Frequencies of KIR gene haplotypes; there were 13 haplotypes detected in 87 Han individuals, among them, the most frequent one was type 2 (haplotypeA-2DS4). (3) Frequencies of KIR genotypes: 18 kinds of the combinations of the haplotypes were observed; the most frequent ones were AJ(2,2), AF (1,2). Also, In this study were identified five new genotypes FZ1  2  9  , FZ2  1  16  , FZ3  6  17  , FZ4  4  13   and FZ5  2  6  ,which had not been observed in Caucasians so far.


CONCLUSIONS
These findings suggest that there are distinctive frequencies of KIR gene content, haplotype as well as genotype in Chinese Han population in Shanghai area.
Zhang
Lei
L
Shanghai Second Medical University, Shanghai Institute of Immunology, Shanghai,200025 PR China.
Hsu
Katharine C
KC
Liu
Xiao-Rong
XR
Yang
Jue-qin
JQ
Yao
Fang-juan
FJ
Xu
Ling-di
LD
Dupont
Bo
B
Fan
Li-an
LA
D002681:China; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1; D054344:Receptors, KIR2DL3; D054345:Receptors, KIR2DL4; D054341:Receptors, KIR3DL1; D054347:Receptors, KIR3DL2; D054349:Receptors, KIR3DS1
D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1; D054344:Receptors, KIR2DL3; D054345:Receptors, KIR2DL4; D054341:Receptors, KIR3DL1; D054347:Receptors, KIR3DL2; D054349:Receptors, KIR3DS1