350-kDa royal jelly glycoprotein (apisin), which stimulates proliferation of human monocytes, bears the beta1-3galactosylated N-glycan: analysis of the N-glycosylation site.
While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galbeta1-3GlcNAcbeta1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and (1)H-NMR. The structural analysis showed that the 350-kDa RJGP bears Galbeta1-3GlcNAcbeta1-4(GlcNAcbeta1-2)Manalpha1-3 (Manalpha1-3Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both beta1-3 galactosyl and beta1-4 N-acetylglucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifying an insect glycoprotein bearing the beta1-3 galactosylated N-glycan.
Kimura
Mariko
M
Faculty of Food Culture, Department of Food System, Kurashiki Sakuyo University, Nagao-Tamashima, Japan.
Kimura
Yoshinobu
Y
Tsumura
Kazunori
K
Okihara
Kiyoshi
K
Sugimoto
Hiroyuki
H
Yamada
Hideo
H
Yonekura
Masami
M
D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000494:pharmacology; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D000099219:Royal Jelly
D005227:Fatty Acids; D006023:Glycoproteins; D011134:Polysaccharides; D000099219:Royal Jelly