title: Development of a nonsurgical diagnostic tool for endometriosis based on the detection of endometrial leukocyte subsets and serum CA-125 levels.
abstract: OBJECTIVE
  To determine whether the proportion of several leukocyte subsets is modulated in the endometrium of patients with endometriosis and, if yes, whether it can be used for diagnostic purposes.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Eight clinical institutions of the Montreal area.
  
  
  METHODS
  Women who underwent laparoscopy or laparotomy between 1997 and 2001, who had regular menstrual cycles and were not under hormone treatment for the previous 3 months were selected. This study included 368 women, 173 with surgically confirmed endometriosis and 195 controls with no surgical evidence of endometriosis.
  
  
  METHODS
  
  
  
  METHODS
  Cytometry analysis was used to measure the proportion of several leukocyte subsets among CD45(+) endometrial cells.
  
  
  RESULTS
  The proportion of CD3(+), CD16(+), CD3(-)HLADR(-), CD3(-)CD45RA(-), CD3(+)CD16(-), CD3(+)CD56(-), CD56(-)CD16(+), and CD16b(+) leukocytes was significantly altered in the endometrium of cases compared with controls. A multiple logistic regression model was adjusted with these endometrial leukocytes, serum CA-125 levels, risk factors, and confounders. The diagnostic performance of this predictive model was defined by a specificity of 95% and a sensitivity of 61%. Furthermore, the positive and negative predictive values were 91% and 75%, respectively.
  
  
  CONCLUSIONS
  This predictive model represents a novel diagnostic tool to identify women with a high likelihood of suffering from endometriosis.
authors.affiliation: MetrioGene, Montréal, Québec, Canada.
authors.forename: Danièle
authors.initials: D
authors.lastname: Gagné
authors.forename: Michèle
authors.initials: M
authors.lastname: Rivard
authors.forename: Martin
authors.initials: M
authors.lastname: Pagé
authors.forename: Manon
authors.initials: M
authors.lastname: Lépine
authors.forename: Christèle
authors.initials: C
authors.lastname: Platon
authors.forename: Kamran
authors.initials: K
authors.lastname: Shazand
authors.forename: Patrice
authors.initials: P
authors.lastname: Hugo
authors.forename: Diane
authors.initials: D
authors.lastname: Gosselin
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D018394:CA-125 Antigen / Q000097:blood*; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood* / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D004717:Endometrium / Q000473:pathology*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000473:pathology*; D011237:Predictive Value of Tests; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D018394:CA-125 Antigen