Membrane-associated MMP-2 in human cervical cancer.
Matrixmetalloproteinases (MMPs) are a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. Recent studies have strongly indicated the presence of a functional complex consisting of alpha(v)beta3 integrin, membrane type-1 metalloproteinase-2 (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) on tumor cell surface, which helps the activation of MMP-2. In this article, we report on the association of active MMP-2 with the membrane fraction of human cervical cancer cells. The association of MMP-2 with alpha(v)beta3 integrin and the expression of membrane type MT1-MMP strongly indicate the role of alpha(v)beta3/MT1-MMP/TIMP-2 complex in the activation of MMP-2 in cervical cancer tissue membrane fraction. The membrane-associated activated MMP-2 may have a role in the migration of tumor cells during invasion.
Mitra
Aparna
A
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Kolkata, India.
Chakrabarti
Jayati
J
Chattopadhyay
Nibedita
N
Chatterjee
Amitava
A
D002462:Cell Membrane; D002471:Cell Transformation, Neoplastic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000494:pharmacology*; D009361:Neoplasm Invasiveness; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000503:physiopathology*
D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2