Antiproliferative activity of cardenolides isolated from Streptocaulon juventas.
Sixteen cardenolides, two hemiterpenoids, two phenylpropanoids and a phenylethanoid isolated from the roots of Streptocaulon juventas (LOUR.) MERR. were examined for their antiproliferative activity toward three human-derived (HT-1080 fibrosarcoma, lung A549 adenocarcinoma, cervix HeLa adenocarcinoma) and three murine-derived (colon 26-L5 carcinoma, Lewis lung carcinoma, B16-BL6 melanoma) cell lines. The cardenolides selectively and strongly inhibited proliferation of the HT-1080 (IC(50) values, 0.054-1.6 microM) and A549 (IC(50), 0.016-0.65 microM) cell lines. The characteristic morphological changes and ladder-like DNA fragmentation in those cells treated with the cardenolides indicated the antiproliferative activity was due to the induction of apoptosis.
Ueda
Jun-Ya
JY
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Tezuka
Yasuhiro
Y
Banskota
Arjun Hari
AH
Tran
Quan Le
QL
Tran
Qui Kim
QK
Saiki
Ikuo
I
Kadota
Shigetoshi
S
D000818:Animals; D029062:Apocynaceae; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots
D002298:Cardenolides; D006131:Growth Inhibitors; D010936:Plant Extracts