Effect of Bak Foong pills on exocrine pancreatic-bile secretion.
We have recently demonstrated that Bak Foong Pills (BFP), a well-known Chinese medicine widely used for treating gynecological disorders, stimulates human colonic epithelial anion secretion, which was mediated by intracellular cAMP and Ca(2+). The present study further investigated the effect of BFP on exocrine pancreatic-bile secretion using in vivo and in vitro approaches. Duodenal infusion of BFP ethanol extract (1 g/kg) in rats produced increases in the volume and protein output of pancreatic-bile juice, but did not affect its pH. Surgical ablation of vagal neural pathway slightly reduced the effect of BFP on the protein output and volume, indicating that the vagal nerve pathway was not the major player in medicating the effect of BFP on exocrine pancreatic-bile secretion. Using CAPAN-1 cell line, a human pancreatic duct cell line, in conjunction with the short-circuit current (I(SC)) measurements, we further demonstrated that BFP could directly stimulate pancreatic HCO(3)(-) secretion. Basolateral addition of BFP (600 microg/ml) produced averaged charges transported of 2100+/-382.5 microC/cm(2), which was blocked by apical addition of Cl(-) channel blocker. Removal of HCO(3)(-) from the Krebs-Henseleit (K-H) solution inhibited the BFP-induced I(SC) by more than 95%. The present results suggest that BFP could improve digestive function by stimulating pancreatic protein and HCO(3)(-) secretion.
Tang
Ning
N
Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong.
Zhu
Jin Xia
JX
Zhao
Wen Chao
WC
Xing
Ying
Y
Gou
Yu Lin
YL
Rowlands
Dewi Kenneth
DK
Chung
Yiu Wa
YW
Chan
Hsiao Chang
HC
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D001652:Bile Ducts / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005088:Exocrine Glands / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D010183:Pancreatic Ducts / Q000187:drug effects / Q000378:metabolism; D010189:Pancreatic Juice / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
C508116:Bak Foong Pills; D001647:Bile Acids and Salts; D004365:Drugs, Chinese Herbal