Cross-linking of Escherichia coli formamidopyrymidine-DNA glycosylase to DNA duplexes containing photoactivatable phenyl(trifluoromethyl)diazirine groups.
New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized. Their substrate properties were investigated. Using photocross-linking technique, we established the presence of contacts of two nucleosides located near the oxoG with amino acids from the Fpg protein. The cross-linking efficiency achieved 10%.
Taranenko
M V
MV
Chemistry Department, Moscow State University, Moscow, Russia.
Sumbatyan
N V
NV
Mtchedlidze
M T
MT
Kuznetsova
S A
SA
D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D043382:DNA-Formamidopyrimidine Glycosylase / Q000378:metabolism*; D003978:Diazomethane; D004926:Escherichia coli / Q000201:enzymology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D003978:Diazomethane; D004247:DNA; D043382:DNA-Formamidopyrimidine Glycosylase