title: The Arabidopsis KAKTUS gene encodes a HECT protein and controls the number of endoreduplication cycles.
abstract: In animals and plants, many cell types switch from mitotic cycles to endoreduplication cycles during differentiation. Little is known about the way in which the number of endoreduplication cycles is controlled in such endopolyploid cells. In this study we have characterized at the molecular level three mutations in the Arabidopsis gene KAKTUS ( KAK), which were previously shown specifically to repress endoreduplication in trichomes. We show that KAK is also involved in the regulation of the number of endoreduplication cycles in various organs that are devoid of trichomes. KAK encodes a protein with sequence similarity to HECT domain proteins. As this class of proteins is known to be involved in ubiquitin-mediated protein degradation, our finding suggests that the number of endoreduplication cycles that occur in several cell types is controlled by this pathway. The KAK gene defines a monophylogenetic subgroup of HECT proteins that also contain Armadillo-like repeats.
authors.affiliation: UMR5575, Laboratoire de Génétique Moléculaire des Plantes, Université J.Fourier, 38041 Grenoble 9, France.
authors.forename: A
authors.initials: A
authors.lastname: El Refy
authors.forename: D
authors.initials: D
authors.lastname: Perazza
authors.forename: L
authors.initials: L
authors.lastname: Zekraoui
authors.forename: J-G
authors.initials: JG
authors.lastname: Valay
authors.forename: N
authors.initials: N
authors.lastname: Bechtold
authors.forename: S
authors.initials: S
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Hülskamp
authors.forename: M
authors.initials: M
authors.lastname: Herzog
authors.forename: J-M
authors.initials: JM
authors.lastname: Bonneville
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D019295:Computational Biology; D018548:Cotyledon / Q000378:metabolism; D017931:DNA Primers; D017343:Genes, Plant; D018546:Hypocotyl / Q000378:metabolism; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D044767:Ubiquitin-Protein Ligases