Structure of the O-polysaccharide leads to classification of Proteus penneri 31 in Proteus serogroup O19.
O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide (LPS) of Proteus penneri strain 31. Sugar and methylation analyses along with NMR spectroscopic studies, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C and 1H,31P HMQC experiments, demonstrated the following structure of the polysaccharide: [carbohydrate structure: see text] where FucNAc is 2-acetamido-2,6-dideoxygalactose and EtnP is 2-aminoethyl phosphate. The polysaccharide studied has the same carbohydrate backbone as the O-polysaccharide of Proteus vulgaris O19. Based on this finding and close serological relatedness of the LPS of the two strains, it is proposed to classify P. penneri 31 in Proteus serogroup O19 as an additional subgroup. In contrast, D-GlcNAc6PEtn and alpha-L-FucNAc-(1-->3)-D-GlcNAc shared with a number of other Proteus O-polysaccharides could not provide any significant cross-reactivity of the corresponding LPS with rabbit polyclonal O-antiserum against P. penneri 31.
Kondakova
Anna N
AN
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Zych
Krystyna
K
Senchenkova
Sof'ya N
SN
Zabłotni
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Sidorczyk
Zygmunt
Z
D000818:Animals; D015373:Bacterial Typing Techniques; D002240:Carbohydrate Sequence; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011511:Proteus / Q000737:chemistry* / Q000145:classification*; D044095:Proteus penneri / Q000737:chemistry* / Q000145:classification*; D012703:Serotyping
D008070:Lipopolysaccharides; D019081:O Antigens; C430212:lipopolysaccharide, Proteus