title: High-performance capillary electrophoresis with indirect UV detection for determination of alpha-galactosides in Leguminosae and Brassicaceae.
abstract: A rapid, easy, and reproducible capillary electrophoresis method for determination of raffinose family oligosaccharides (alpha-galactosides) was developed. Sucrose, raffinose, stachyose, verbascose, and ajugose were determined with indirect UV detection at moderate alkaline pH 9.2, using pyridine-2,6-dicarboxylic acid as background electrolyte in a sodium tetraborate buffer with added cetyltrimethylammonium bromide. The separation efficiency measured by the number of theoretical plates (N) ranged from 1.4 x 10(5) to 2.3 x 10(5). The precision of the method, measured by the relative standard deviation (RSD), was less than 0.53% for the migration times and better than 3.4% for normalized areas (NA), considering all sugars except verbascose (RSD(NA) = 11.8%). Detection limits were about 110 microg/mL, corresponding to 150-320 microM. Relative response factors (RRF) were calculated on the basis of linearity studies and used for quantification of alpha-galactosides in a lupine sample (Lupinus angustifolius).
authors.affiliation: Chemistry Department, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
authors.forename: Keld E
authors.initials: KE
authors.lastname: Andersen
authors.forename: Charlotte
authors.initials: C
authors.lastname: Bjergegaard
authors.forename: Peter
authors.initials: P
authors.lastname: Møller
authors.forename: Jens C
authors.initials: JC
authors.lastname: Sørensen
authors.forename: Hilmer
authors.initials: H
authors.lastname: Sørensen
mesh_terms: D019607:Brassicaceae / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005697:Galactosides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D031297:Lupinus / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis; D011887:Raffinose / Q000032:analysis; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D005697:Galactosides; D009844:Oligosaccharides; C005695:stachyose; D013395:Sucrose; D011887:Raffinose