Development of highly regenerable callus lines and biolistic transformation of turf-type common bermudagrass [Cynodon dactylon (L.) Pers.].
Common bermudagrass, Cynodon dactylon, is a widely used warm-season turf and forage species in the temperate and tropical regions of the world. Improvement of bermudagrass via biotechnology depends on improved tissue culture responses, especially in plant regeneration, and a successful scheme to introduce useful transgenes. When the concentration of 6-benzylaminopurine was adjusted in the culture medium, yellowish, compact calluses were observed from young inflorescence tissue culture of var. J1224. Nine long-term, highly regenerable callus lines (including a suspension-cultured line) were subsequently established, of which six were used for biolistic transformation. Five independent transgenic events, with four producing green plants, were obtained following hygromycin B selection from one callus line. Three transgenic events displayed resistance to the herbicide glufosinate, and one of these showed beta-glucuronidase activity since the co-transformation vector used in the experiments contained both the gusA and bar genes.
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Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA.
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D019470:Biolistics / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D002460:Cell Line; D031744:Cynodon / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D035264:Flowers / Q000235:genetics / Q000502:physiology; D006921:Hygromycin B / Q000302:isolation & purification; D012038:Regeneration
D006921:Hygromycin B