Human antibodies from immunized donors are protective against anthrax toxin in vivo.
A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.
Wild
Martha A
MA
Alexion Antibody Technologies, Inc., 3985 Sorrento Valley Blvd., San Diego, California 92121, USA.
Xin
Hong
H
Maruyama
Toshiaki
T
Nolan
Mary Jean
MJ
Calveley
Peter M
PM
Malone
John D
JD
Wallace
Mark R
MR
Bowdish
Katherine S
KS
D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000276:immunology* / Q000633:toxicity; D001782:Blood Donors; D006801:Humans; D007109:Immunity / Q000502:physiology; D007114:Immunization / Q000379:methods; D007140:Immunoglobulin Fab Fragments / Q000097:blood* / Q000276:immunology*; D019151:Peptide Library; D051381:Rats
D000906:Antibodies; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; C030325:anthrax toxin