Suppression of exonucleolytic degradation of double-stranded DNA and inhibition of Exonuclease III by PNA.
Degradation of double-stranded DNA by Exonuclease III in the presence of complementary anti-parallel PNA was studied. It was found for the first time that the PNA suppresses the degradation of dsDNA in a sequence-specific manner as well as inhibits the activity of Exonuclease III in a non-specific way.
Slaitas
A
A
Division of Organic and Bioorganic Chemistry, MBB, Karolinska Institute, Stockholm, Sweden.
Ander
C
C
Földes-Papp
Z
Z
Rigler
R
R
Yeheskiely
E
E
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D020135:Peptide Nucleic Acids; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III