title: Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies.
abstract: The purpose of the present study was to clarify the influence of tissue preparation on the high-frequency acoustic properties by comparing the acoustic properties of the formalin-fixed, paraffin-embedded, deparaffinized sections and formalin-fixed, frozen sections for two types of fat-containing renal cancer and fat-free renal oncocytoma using a SAM. There was no significant difference for the sound speed among the clear cell, granular cell renal cancer and oncocytoma in either groups, but the attenuation constant was significantly higher for the frozen than for that of the paraffin section in fat-containing renal cancer. In fat-free oncocytoma, there was no significant difference for the attenuation constant in either group. The data suggest that the fat component, which had been eluted by paraffinization, is stored and the true acoustic properties of the tissue can be measured in frozen section.
authors.affiliation: Department of Medical Engineering and Cardiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. sa.ultra@idac.tohoku-u.ac.jp
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Sasaki
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Saijo
authors.forename: Motonao
authors.initials: M
authors.lastname: Tanaka
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nitta
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000000981:diagnostic imaging*; D018249:Adenoma, Oxyphilic / Q000000981:diagnostic imaging*; D005557:Formaldehyde; D005629:Frozen Sections; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D007680:Kidney Neoplasms / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D033401:Microscopy, Acoustic / Q000295:instrumentation; D016612:Paraffin Embedding; D016707:Tissue Fixation / Q000379:methods; D014159:Transducers
chemical_list: D005557:Formaldehyde