Establishment and characterization of unique cell lines derived from pyothorax-associated lymphoma which develops in long-standing pyothorax and is strongly associated with Epstein-Barr virus infection.
Malignant lymphoma frequently develops in the pleural cavity of patients with over 20 years' history of pyothorax. The term pyothorax-associated lymphoma (PAL) has been proposed for this type of tumor. We established four novel lymphoma cell lines (OPL-3, -4, -5, and -7) from four patients with PAL. Characteristics of the four cell lines are as follows: B-cell nature with defective expression of B-cell and T-cell surface antigens, monoclonal pattern of Epstein-Barr virus (EBV) infection in lymphoma cells (thus indicating an etiological role of EBV for lymphomagenesis), complicated chromosomal abnormalities with numerous structural and numerical abnormalities, and occasional but distinct genome instability. These abnormalities in cell character might be caused by the specific circumstances of PAL lymphomagenesis, i.e., chronic inflammation. Thus, PAL cell lines could be useful in analysis of molecular mechanisms leading to malignancy in chronic inflammation.
Takakuwa
Tetsuya
T
Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Luo
Wen-Juan
WJ
Ham
Maria Francisca
MF
Mizuki
Masao
M
Iuchi
Keiji
K
Aozasa
Katsuyuki
K
D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015139:Blotting, Southern; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D016724:Empyema, Pleural / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D005260:Female; D005434:Flow Cytometry; D015321:Gene Rearrangement / Q000235:genetics; D019672:Genes, T-Cell Receptor / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000150:complications / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype
D007143:Immunoglobulin Heavy Chains