Cloning of European eel (Anguilla anguilla) FSH-beta subunit, and expression of FSH-beta and LH-beta in males and females after sex determination.
The European eel (Anguilla anguilla) is a catadromic teleost species with a complex life cycle, both in sea and freshwater environments. The sex determination phase of gonadal development occurs in a freshwater environment. Polymorphism occurs in increasing rates with respect to gender. While males stop growing at approximately 150 g, females continue to grow to being much larger. In this study, we cloned the cDNA FSH-beta subunit of the European eel (A. anguilla), and measured the mRNA levels of FSH-beta and LH-beta in males and females after sex determination. The FSH-beta subunit cDNA consisted of 1068 bp, encoding a 127 amino acid peptide. A comparison between European and Japanese eels of the FSH-beta amino acid sequence showed 98% similarity.
Degani
G
G
MIGAL-Galilee Technology Center, Migal P.O. Box 831, Kiryat Shmona 10200, Israel. gad@migal.co.il
Goldberg
D
D
Tzchori
I
I
Hurvitz
A
A
Yom Din
S
S
Jackson
K
K
D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D020869:Gene Expression Profiling; D006066:Gonads / Q000166:cytology / Q000254:growth & development; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012727:Sex Characteristics; D012733:Sex Differentiation
D018076:DNA, Complementary; D037201:Follicle Stimulating Hormone, beta Subunit; D037101:Luteinizing Hormone, beta Subunit; D021122:Protein Subunits