title: Modulation of human sperm function by peritoneal fluid.
abstract: OBJECTIVE
  To examine the effect of peritoneal fluid on various parameters of sperm function in vitro.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Basic research laboratory.
  
  
  METHODS
  Semen samples were obtained from normozoospermic volunteers (n = 43). Peritoneal fluids were aspirated laparoscopically from women with unexplained infertility (n = 14). Follicular fluid and oocytes were collected from patients undergoing IVF-ET.
  
  
  METHODS
  Sperm incubated under capacitating conditions were exposed to peritoneal fluid, and functional variables were evaluated in vitro.
  
  
  METHODS
  Sperm viability and motility, follicular fluid and calcium ionophore-induced acrosome reactions, protein tyrosine phosphorylation, expression of D-mannose binding sites, and ability of sperm to interact with zona pellucida.
  
  
  RESULTS
  Exposure of sperm to peritoneal fluid for up to 6 hours did not affect sperm viability or motility. Unlike follicular fluid, peritoneal fluid did not induce the acrosome reaction. Moreover, incubation of sperm with > or =20% v/v peritoneal fluid for 1 hour prevented the follicular fluid and the ionophore-induced acrosome reaction. Although treatment with peritoneal fluid allowed protein tyrosine phosphorylation during capacitation, it resulted in a significant decrease in the expression of D-mannose binding sites and sperm-zona pellucida binding.
  
  
  CONCLUSIONS
  Peritoneal fluid maintains sperm survival and decreases sperm ability to respond to inducers of the acrosome reaction and bind to the zona pellucida in vitro, indicating that this fluid might modulate sperm function in vivo.
authors.affiliation: Laboratorio de Estudios Reproductivos, Cátedra de Bioquímica Clínica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosaria, Argentina. reprolab@citynet.net.ar
authors.forename: María José
authors.initials: MJ
authors.lastname: Munuce
authors.forename: Clara I
authors.initials: CI
authors.lastname: Marín-Briggiler
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Caille
authors.forename: César L
authors.initials: CL
authors.lastname: Berta
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Cuasnicú
authors.forename: Lida
authors.initials: L
authors.lastname: Morisoli
mesh_terms: D020101:Acrosome Reaction; D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D001665:Binding Sites; D002470:Cell Survival; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D007246:Infertility / Q000503:physiopathology*; D008297:Male; D008358:Mannose / Q000378:metabolism; D010766:Phosphorylation; D011446:Prospective Studies; D013075:Sperm Capacitation; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D014443:Tyrosine; D008358:Mannose