title: Suppressive effect of the SOS-inducing activity of chemical mutagen by citric acid esters from Prunus mume Sieb. Et Zucc. using the Salmonella typhimurium TA1535/pSK1002 umu test.
abstract: A methanol extract from Prunus mume Sieb. Et Zucc. showed a suppressive effect of the SOS-inducing activity on the mutagen 3-amino-1,4-dimethyl-SH-pyrido[4,3-b]indole(Trp-P-1) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with hexane, dichloromethane, ethyl acetate, butanol and water. The dichloromethane and ethyl acetate fractions showed suppressive effect. Suppressive compounds were isolated by silica gel column chromatography and identified as trimethyl citrate (1) and dimethyl citrate (2) by GC-MS, IR and 1H and 13C-NMR spectroscopy. Compounds 1 and 2 suppressed 51 and 39% of the SOS-inducing activity at a concentration of 2.0 micromol/mL.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka-shi, Osaka 577-8502, Japan. miyazawa@apch.kindai.ac.jp
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yamada
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
mesh_terms: D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002243:Carbolines / Q000633:toxicity*; D002951:Citrates / Q000302:isolation & purification / Q000494:pharmacology*; D019343:Citric Acid / Q000031:analogs & derivatives*; D004249:DNA Damage; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology; D027861:Prunus / Q000737:chemistry*; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000235:genetics; D012997:Solvents
chemical_list: D016587:Antimutagenic Agents; D002243:Carbolines; D002951:Citrates; D009153:Mutagens; D010936:Plant Extracts; D012997:Solvents; C478839:dimethyl citrate; D019343:Citric Acid; C478838:trimethyl citrate; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; D000432:Methanol