title: Behavioral reinforcement of long-term potentiation in rat dentate gyrus in vivo is protein synthesis-dependent.
abstract: A transient, protein synthesis-independent long-term potentiation (early-LTP, <4 h) can be reinforced into a maintained protein synthesis-dependent late-LTP (>4 h) by specific electrical stimulation of limbic structures (J. Neurosci. 21 (2001) 3697). Similarly, LTP-modulation can be obtained by behavioral stimuli with strong motivational content. However, the requirement of protein synthesis during behavioral reinforcement has not been shown so far. Thus, we have studied here this specific question using a behavioral reinforcement protocol, i.e. allowing water-deprived animals to drink 15 min after induction of early-LTP. This procedure transformed early-LTP into late-LTP. Anisomycin, a reversible protein synthesis inhibitor, abolished behavioral LTP-reinforcement. These results demonstrate that behavioral reinforcement depends on protein synthesis.
authors.affiliation: International Center for Neurological Restoration, Avenue 25 # 15805, Playa, Havana, Cuba.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Bergado
authors.forename: W
authors.initials: W
authors.lastname: Almaguer-Melian
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kostenko
authors.forename: Sabine
authors.initials: S
authors.lastname: Frey
authors.forename: Julietta U
authors.initials: JU
authors.lastname: Frey
mesh_terms: D000200:Action Potentials; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001522:Behavior, Animal; D018891:Dentate Gyrus / Q000502:physiology*; D004558:Electric Stimulation; D017774:Long-Term Potentiation; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012054:Reinforcement, Psychology; D014868:Water Deprivation
chemical_list: D011500:Protein Synthesis Inhibitors; D000841:Anisomycin