title: Universal labeling chemistry for nucleic acid detection on DNA-arrays.
abstract: We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.
authors.affiliation: BioMérieux, Advanced Technology, Marcy l'Etoile, France. eloy.bernalmendez@eu.biomerieux.com
authors.forename: E
authors.initials: E
authors.lastname: Bernal-Méndez
authors.forename: C
authors.initials: C
authors.lastname: Tora
authors.forename: I
authors.initials: I
authors.lastname: Sothier
authors.forename: M
authors.initials: M
authors.lastname: Kotera
authors.forename: A
authors.initials: A
authors.lastname: Troesch
authors.forename: A
authors.initials: A
authors.lastname: Laayoun
mesh_terms: D001391:Azo Compounds; D019921:Biotinylation; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D001391:Azo Compounds; D005456:Fluorescent Dyes; D004247:DNA