Optical imaging of metastatic tumors using a folate-targeted fluorescent probe.
We describe the use of a tumor targeting ligand, the vitamin folic acid, to deliver an attached fluorescent probe to both primary and metastatic tumors overexpressing the folate receptor. Upon laser excitation, derived images of normal tissues generally show little or no fluorescence, whereas images of folate receptor-expressing tumors display bright fluorescence that can be easily distinguished from adjacent normal tissue. Furthermore, metastatic tumor loci of submillimeter size can also be visualized without the aid of image processing or enhancement. The sharp distinction between tumor and normal tissues provided by this technique could find application in the localization and resection of tumor tissue during surgery or in the enhanced endoscopic detection and staging of cancers.
Kennedy
Michael D
MD
Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, USA.
Jallad
Karim N
KN
Thompson
David H
DH
Ben-Amotz
Dor
D
Low
Philip S
PS
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods*; D009362:Neoplasm Metastasis / Q000473:pathology; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D009382:Neoplasms, Unknown Primary / Q000378:metabolism / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D011956:Receptors, Cell Surface; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary
D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D005492:Folic Acid