title: Correlation between the occurrence of 1H-MRS lipid signal, necrosis and lipid droplets during C6 rat glioma development.
abstract: The aim of this study was to investigate the possible correlation between the 1H MRS mobile lipid signal, necrosis and lipid droplets in C6 rat glioma. First, the occurrence of necrosis and lipid droplets was determined during tumor development, by a histological analysis performed on 34 rats. Neither necrosis nor lipid droplets were observed before 18 days post-implantation. At later stages of development, both necrosis and lipid droplets were apparent, the lipid droplets being mainly located within the necrotic areas. Using a second group of eight rats, a temporal correlation was evidenced between mobile lipid signal detected by in vivo single-voxel one- (136 ms echo time) and two-dimensional J-resolved 1H MR spectroscopy, and the presence of necrosis and lipid droplets on the histological sections obtained from the brains of the same rats. Finally, spatial distribution of the mobile lipid signal was analyzed by chemical-shift imaging performed on a third group of eight animals, at the end of the tumor growth. The spectroscopic image corresponding to the resonance of mobile lipids had its maximum intensity in the center of the tumor where necrotic regions were observed on the histological sections. These necrotic areas contained large amounts of lipid droplets. All these results suggest that mobile lipids detected in vivo by 1H MRS (136 ms echo time) in C6 rat brain glioma arise mainly from lipid droplets located in necrosis.
authors.affiliation: Laboratoire mixte INSERM U438/Université Joseph Fourier, Laboratoire de Recherche Correspondant du CEA, Centre Hospitalier Universitaire, Grenoble, France.
authors.forename: Sonja
authors.initials: S
authors.lastname: Zoula
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Hérigault
authors.forename: Anne
authors.initials: A
authors.lastname: Ziegler
authors.forename: Régine
authors.initials: R
authors.lastname: Farion
authors.forename: Michel
authors.initials: M
authors.lastname: Décorps
authors.forename: Chantal
authors.initials: C
authors.lastname: Rémy
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D009336:Necrosis; D009368:Neoplasm Transplantation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D013223:Statistics as Topic; D013997:Time Factors
chemical_list: D011522:Protons; D019344:Lactic Acid