Acute inflammatory response induced by Helicobacter pylori in the rat air pouch.
Infection by Helicobacter pylori elicits persistent neutrophil infiltration in the gastric mucosa and stimulates the release of substances that may contribute to the establishment of gastritis. In this study, we used the rat air pouch model to evaluate the acute inflammatory response to H. pylori, in vivo. A pronounced neutrophil infiltration was observed 6 h and 12 h after the injection of H. pylori into the air pouch. Strains with different genotypes were able to induce cellular influx. This response was dependent upon the amount of bacteria injected and still occurred when heat-killed bacteria were employed. An increase in prostaglandin E(2) levels was observed, indicating that H. pylori induced cyclooxygenase 2 in this model. The production of interleukin-1 beta and tumor necrosis factor-alpha by leukocytes was also enhanced, suggesting that this model may be useful for studying the direct activation of neutrophils by H. pylori in vivo.
Gambero
Alessandra
A
Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Av São Francisco de Assis 218, 12916-900, Bragança Paulista SP, Brazil.
Becker
Tagliane L
TL
Gurgueira
Sonia A
SA
Benvengo
Yune H B
YH
Ribeiro
Marcelo L
ML
de Mendonça
Sergio
S
Pedrazzoli
José
J
D000818:Animals; D015232:Dinoprostone / Q000032:analysis; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000737:chemistry / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D007249:Inflammation; D007375:Interleukin-1 / Q000032:analysis; D007958:Leukocyte Count; D007962:Leukocytes; D008297:Male; D020556:Neutrophil Infiltration; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone