Molecular biology of myopia.
Experiments in animal models of myopia have emphasised the importance of visual input in emmetropisation but it is also evident that the development of human myopia is influenced to some degree by genetic factors. Molecular genetic approaches can help to identify both the genes involved in the control of ocular development and the potential targets for pharmacological intervention. This review covers a variety of techniques that are being used to study the molecular biology of myopia. In the first part, we describe techniques used to analyse visually induced changes in gene expression: Northern Blot, polymerase chain reaction (PCR) and real-time PCR to obtain semi-quantitative and quantitative measures of changes in transcription level of a known gene, differential display reverse transcription PCR (DD-RT-PCR) to search for new genes that are controlled by visual input, rapid amplification of 5' cDNA (5'-RACE) to extend the 5' end of sequences that are regulated by visual input, in situ hybridisation to localise the expression of a given gene in a tissue and oligonucleotide microarray assays to simultaneously test visually induced changes in thousands of transcripts in single experiments. In the second part, we describe techniques that are used to localise regions in the genome that contain genes that are involved in the control of eye growth and refractive errors in mice and humans. These include quantitative trait loci (QTL) mapping, exploiting experimental test crosses of mice and transmission disequilibrium tests (TDT) in humans to find chromosomal intervals that harbour genes involved in myopia development. We review several successful applications of this battery of techniques in myopia research.
Schaeffel
Frank
F
University Eye Hospital, Section for Neurobiology of the Eye,Calwestr 7/1, 72076, Tubingen, Germany.
Simon
Perikles
P
Feldkaemper
Marita
M
Ohngemach
Sibylle
S
Williams
Robert W
RW
D000818:Animals; D002874:Chromosome Mapping; D006801:Humans; D017403:In Situ Hybridization; D015810:Linkage Disequilibrium; D008967:Molecular Biology; D009216:Myopia / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis; D040641:Quantitative Trait Loci; D014158:Transcription, Genetic; D014785:Vision, Ocular