RNA cleaving '10-23' DNAzymes with enhanced stability and activity.
'10-23' DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications in vivo, they have to be stabilised against nucleolytic attack by the introduction of modified nucleotides without obstructing cleavage activity. In this study, we optimise the design of a DNAzyme targeting the 5'-non-translated region of the human rhinovirus 14, a common cold virus, with regard to its kinetic properties and its stability against nucleases. We compare a large number of DNAzymes against the same target site that are stabilised by the use of a 3'-3'-inverted thymidine, phosphorothioate linkages, 2'-O-methyl RNA and locked nucleic acids, respectively. Both cleavage activity and nuclease stability were significantly enhanced by optimisation of arm length and content of modified nucleotides. Furthermore, we introduced modified nucleotides into the catalytic core to enhance stability against endonucleolytic degradation without abolishing catalytic activity. Our findings enabled us to establish a design for DNAzymes containing nucleotide modifications both in the binding arms and in the catalytic core, yielding a species with up to 10-fold enhanced activity and significantly elevated stability against nucleolytic cleavage. When transferring the design to a DNAzyme against a different target, only a slight modification was necessary to retain activity.
Schubert
Steffen
S
Free University Berlin, Institute of Chemistry (Biochemistry), Thielallee 63, D-14195 Berlin, Germany.
Gül
Deniz C
DC
Grunert
Hans-Peter
HP
Zeichhardt
Heinz
H
Erdmann
Volker A
VA
Kurreck
Jens
J
D001665:Binding Sites / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D009154:Mutation; D009691:Nucleic Acid Denaturation; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012229:Rhinovirus / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D009843:Oligoribonucleotides; D012367:RNA, Viral; C402582:RNA-cleaving DNA 10-23; D015719:Single-Strand Specific DNA and RNA Endonucleases