Molecular tools for targeted imaging and therapy of cancer.
Here we review an approach to the design and production of antibody/ligand pairs for use in cell targeting procedures, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate. By eliminating the dissociation of the ligand from the antibody, we have made the affinity functionally infinite. This methodology is applicable to other biological binding pairs.
Meares
Claude F
CF
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. cfmeares@ucdavis.edu
Chmura
A J
AJ
Orton
Molly S
MS
Corneillie
Todd M
TM
Whetstone
Paul A
PA
D000818:Animals; D000906:Antibodies / Q000187:drug effects* / Q000276:immunology; D001360:Avidin / Q000493:pharmacokinetics; D001710:Biotin / Q000493:pharmacokinetics; D006801:Humans; D008674:Metals, Rare Earth; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D011859:Radiography; D011868:Radioisotopes; D011877:Radionuclide Imaging
D000906:Antibodies; D008674:Metals, Rare Earth; D011868:Radioisotopes; D001360:Avidin; D001710:Biotin