Simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4'-hydroxy-3'-methoxyacetophenone in urine by capillary gas chromatography.
A method for the simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4'-hydroxy-3'-methoxyacetophenone in urine has been described. The metabolites were analyzed after enzymatic hydrolysis and extraction on octyl (C8) cartridges by using gas chromatography with flame ionization detection and a 5/95% copolymer of diphenyl-poly(dimethylsiloxane) capillary column. Methoxyphenols were well separated within 12 min. Recovery was over 90% in the range from 0.5 to 20 microg/ml; the detection limit was varying in the range of 0.05-0.11 microg/ml. The relative standard deviations and the accuracy were in the range of 3.1-15.5 and 2.4-16.0%, respectively.
Bieniek
Grazyna
G
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland. maja@slam.katowice.pl
D000098:Acetophenones / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D003408:Cresols / Q000652:urine*; D006139:Guaiacol; D006801:Humans; D016273:Occupational Exposure; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000098:Acetophenones; D003408:Cresols; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; D006139:Guaiacol; C056165:acetovanillone; C025390:creosol