title: Smooth muscle cell outgrowth from coronary atherectomy specimens in vitro is associated with less time to restenosis and expression of a key Transcription factor KLF5/BTEB2.
abstract: Atherectomy specimens offer an opportunity to study the biology of coronary artery lesions. We cultured smooth muscle cells (SMCs) from specimens obtained from 24 patients with coronary restenosis after angioplasty to study the relationship between activity of SMCs (in vitro outgrowth) and the time course of restenosis. We also examined expression of a Kruppel-like zinc-finger transcription factor 5 (KLF; also known as BTEB2 and IKLF), which is markedly induced in activated SMCs, in the same specimens. SMC outgrowth was observed in 9 of 24 specimens (37.5%). Restenosis occurred sooner (p < 0.01) in patients whose specimens showed outgrowth compared to those whose specimens showed no outgrowth. Immunostaining for KLF5 was more common in specimens with outgrowth (89 vs. 20%, p < 0.01). These data suggest that the number of activated SMCs in lesions may determine in vitro outgrowth and also affect the time to restenosis.
authors.affiliation: Department of General Medicine, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. sakamoth@med.gunma-u.ac.jp
authors.forename: Hironosuke
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Sakamaki
authors.forename: Tsugiyasu
authors.initials: T
authors.lastname: Kanda
authors.forename: Yo-ichi
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Sawada
authors.forename: Mahito
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Sato
authors.forename: Yuko
authors.initials: Y
authors.lastname: Oyama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nakano
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Takase
authors.forename: Akira
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Ryozo
authors.initials: R
authors.lastname: Nagai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurabayashi
mesh_terms: D000368:Aged; D017225:Atherectomy, Coronary; D002478:Cells, Cultured; D023903:Coronary Restenosis / Q000453:epidemiology / Q000378:metabolism* / Q000601:surgery*; D003331:Coronary Vessels / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology*; D012307:Risk Factors; D013223:Statistics as Topic; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis*
chemical_list: C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors; D015534:Trans-Activators