title: [Effects of nitric oxide synthase inhibitor on dentate gyrus neurogenesis after diffuse brain injury in adult rats].
abstract: OBJECTIVE
  To investigate the effects of selective nitric oxide synthase (NOS) inhibitors on dentate gyrus (DG) neurogenesis after diffuse brain injury (DBI) in adult rats.
  
  
  METHODS
  DBI models were established in adult male SD rats, followed by systemic bromodeoxyuridine (BrdU) labeling of the dividing cells and immunohistochemical assay of the proliferation rates of neural precursor cells in the DG for comparison between NOS inhibitor (7-nitroindazole and Aminoguanidine) groups and the corresponding control groups at various time points after DBI.
  
  
  RESULTS
  Intraperitoneal administration of 7-nitroindazole significantly reduced the number of BrdU-labeled cells in the DG of adult rats 2, 4 and 6 d after DBI (P<0.05). Aminoguanidine also significantly inhibited the proliferation of neural precursor cells in the DG induced by DBI at various time points (P<0.01).
  
  
  CONCLUSIONS
  The activation of NOS after DBI may be an important regulatory factor for DG neurogenesis in adult rats, and NO generated by nNOS is probably involved mainly in the early stage of enhanced neurogenesis after DBI, while the NO from iNOS might participated primarily in the later stages.
authors.affiliation: Department of Neurosurgery, Jiangxi Headquater Hospital of Chinese People's Armed Police Forces, Nanchang 330001, China.
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Cheng
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wang
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Sun
authors.forename: Zu-jue
authors.initials: ZJ
authors.lastname: Cheng
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Xu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D018891:Dentate Gyrus / Q000187:drug effects* / Q000503:physiopathology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; D019001:Nitric Oxide Synthase