A catalogue of molecular, physiological and symbiotic properties of soybean-nodulating rhizobial strains from different soybean cropping areas of China.
We have analysed 198 fast-growing soybean-nodulating rhizobial strains from four different regions of China for the following characteristics: generation time; number of plasmids; lipopolysaccharide (LPS), nodulation factors (LCOs) and PCR profiles; acidification of growth medium; capacity to grow at acid, neutral, and alkaline pH; growth on LC medium; growth at 28 and 37 degrees C; melanin production capacity; Congo red absorption and symbiotic characteristics. These unbiased analyses of a total subset of strains isolated from specific soybean-cropping areas (an approach which could be called "strainomics") can be used to answer various biological questions. We illustrate this by a comparison of the molecular characteristics of five strains with interesting symbiotic properties. From this comparison we conclude, for instance, that differences in the efficiency of nitrogen fixation or competitiveness for nodulation of these strains are not apparently related to differences in Nod factor structure.
Thomas-Oates
J
J
Department of Chemistry, University of York, Heslington, York, UK.
Bereszczak
J
J
Edwards
E
E
Gill
A
A
Noreen
S
S
Zhou
J C
JC
Chen
M Z
MZ
Miao
L H
LH
Xie
F L
FL
Yang
J K
JK
Zhou
Q
Q
Yang
S S
SS
Li
X H
XH
Wang
L
L
Spaink
H P
HP
Schlaman
H R M
HR
Harteveld
M
M
Díaz
C L
CL
van Brussel
A A N
AA
Camacho
M
M
Rodríguez-Navarro
D N
DN
Santamaría
C
C
Temprano
F
F
Acebes
J M
JM
Bellogín
R A
RA
Buendía-Clavería
A M
AM
Cubo
M T
MT
Espuny
M R
MR
Gil
A M
AM
Gutiérrez
R
R
Hidalgo
A
A
López-Baena
F J
FJ
Madinabeitia
N
N
Medina
C
C
Ollero
F J
FJ
Vinardell
J M
JM
Ruiz-Sainz
J E
JE
D001426:Bacterial Proteins / Q000032:analysis; D002681:China; D003224:Congo Red / Q000378:metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis; D008543:Melanins / Q000096:biosynthesis; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D013025:Glycine max / Q000382:microbiology*; D013559:Symbiosis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D008070:Lipopolysaccharides; D008543:Melanins; D012336:RNA, Ribosomal, 16S; C023023:lipid-linked oligosaccharides; D003224:Congo Red