title: Preliminary 2-D chromatographic investigation of the human stem cell proteome.
abstract: Stem cells represent a promising tool for the treatment of various hematopoietic diseases. In order to identify stem cell-specific proteins, the proteome of human stem cells from umbilical cord blood was explored for the first time. For this purpose, the crude lysate of 4 x 10(5) CD34+ cells was subjected to in solution trypsin digestion. The resulting peptides were then separated via cation exchange followed by reversed phase chromatography and analyzed by nanospray MS/MS. Database search revealed a total of 215 proteins which could be reliably identified. To obtain a more complete picture of the human stell cell proteome and to also access low abundant proteins, pooling of more than one CD34+ preparations seems necessary in order to increase the cell number and thus the protein content.
authors.affiliation: Lifecord Inc., Graz, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Zenzmaier
authors.forename: Manfred
authors.initials: M
authors.lastname: Kollroser
authors.forename: Bernd
authors.initials: B
authors.lastname: Gesslbauer
authors.forename: Anita
authors.initials: A
authors.lastname: Jandrositz
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Preisegger
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Kungl
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D020543:Proteome / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D013234:Stem Cells / Q000737:chemistry*
chemical_list: D018952:Antigens, CD34; D020543:Proteome