title: CYP330A1 and CYP4C39 enzymes in the shore crab Carcinus maenas: sequence and expression regulation by ecdysteroids and xenobiotics.
abstract: Cytochrome P450 enzymes (CYP enzymes) catalyse important metabolic reactions of exogenous and endogenous substrates, including steroid hormones. Here, we report the first two CYP sequences from the shore crab, Carcinus maenas. Two complete cDNAs isolated from crab hepatopancreas encode CYP enzymes named CYP330A1, the first member of a new family, and CYP4C39. CYP330A1 is closest related to members of the CYP2 family (37.3% identical to mouse CYP2J6) and CYP4C39 is most identical to crayfish CYP4C15 (59.5%). CYP330A1 gene expression was induced in hepatopancreas of male green intermoult crabs by ecdysone and ponasterone A, but also by benzo(a)pyrene and phenobarbital. CYP330A1 induction was not observed in red crabs. The present results indicate that the CYP330A1 enzyme may be involved in ecdysteroid metabolism, presumably catabolism, and in the detoxification of environmental pollutants. Ecdysteroids or xenobiotics did not affect CYP4C39 gene expression. The fact that both ecdysteroids and xenobiotics affect CYP330A1 gene expression indicates that mutual interactions between chemical exposures and endocrine functions may exist in the shore crab.
authors.affiliation: Department of Life Sciences and Chemistry, Roskilde University, P.O. Box 260, 4000 Roskilde, Denmark. rewitz@ruc.dk
authors.forename: Kim
authors.initials: K
authors.lastname: Rewitz
authors.forename: Bjarne
authors.initials: B
authors.lastname: Styrishave
authors.forename: Ole
authors.initials: O
authors.lastname: Andersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D026461:Ecdysteroids / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D015262:Xenobiotics / Q000494:pharmacology*
chemical_list: C000717353:Cyp2j6 protein, mouse; D026461:Ecdysteroids; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; D000090762:Cytochrome P-450 CYP2J2