title: Determination of irinotecan (CPT-11) and SN-38 in human whole blood and red blood cells by liquid chromatography with fluorescence detection.
abstract: An analytical method was developed for the anticancer agent irinotecan (CPT-11) and its main metabolite SN-38 in human whole blood and in red blood cells (RBCs). Sample pretreatment involved deproteinization of whole blood or plasma-diluted RBCs isolated by MESED instruments, with a mixture of aqueous perchloric acid and methanol (1:1, v/v). Separation was carried out using isocratic elution on a Hypersil ODS stationary phase, with detection at excitation and emission wavelengths of 355 and 515 nm, respectively. The lower limit of quantitation (LLQ) in blood was established at 5.00 ng/ml for both compounds, with values for within-run precision (WRP) and between-run precision (BRP) of less than 10%. The method is currently being applied to investigate the blood distribution of CPT-11 and SN-38 in cancer patients.
authors.affiliation: Department of Medical Oncology, Erasmus MC-University Medical Center Rotterdam, P.O. Box 5201, 3008 AE Rotterdam, The Netherlands.
authors.forename: Floris A
authors.initials: FA
authors.lastname: de Jong
authors.forename: Ron H J
authors.initials: RH
authors.lastname: Mathijssen
authors.forename: Peter
authors.initials: P
authors.lastname: de Bruijn
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Loos
authors.forename: Jaap
authors.initials: J
authors.lastname: Verweij
authors.forename: Alex
authors.initials: A
authors.lastname: Sparreboom
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004791:Enzyme Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D000077146:Irinotecan; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D059004:Topoisomerase I Inhibitors
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D059004:Topoisomerase I Inhibitors; D000077146:Irinotecan; D002166:Camptothecin