[Detection of platelet activation by flow cytometry in patients with pre-eclampsia].
OBJECTIVE
To explore the clinical significance of expression alterations of static and activated platelet glycoprotein CD41a, CD62p, and CD63 in patients with pre-eclampsia.


METHODS
We used non-washing immunolabeling technique for fluorescence labeling of the static and activated platelet with CD41a, CD62p, and CD63 monoclonal antibodies of the blood samples from 16 patients with pre-eclampsia, 15 women with normal pregnancies and 20 healthy subjects. The expression of the monoclonal antibody was detected by flow cytometry.


RESULTS
The average static and activated platelet glycoprotein CD41a, CD62p, and CD63 expression levels in patients with pre-eclampsia were apparently higher than those of the normal pregnant women (P<0.01), while the comparison between the latter and the control group did not reveal any significant differences (P>0.05).


CONCLUSIONS
High levels of platelet glycoprotein CD62p and CD63 expressions are present in patients with preeclampsia, suggesting the presence of platelet activation in these patients.
Yi
Zheng-Shan
ZS
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zhou
Shu-Yun
SY
Meng
Fan-Yi
FY
Feng
Ru
R
Liu
Qi-Fa
QF
Lin
Rong
R
D000328:Adult; D015703:Antigens, CD / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D040201:Platelet Membrane Glycoprotein IIb / Q000097:blood; D010980:Platelet Membrane Glycoproteins; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D060149:Tetraspanin 30
D015703:Antigens, CD; C554586:CD63 protein, human; D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30