title: MECC determination of oleanolic acid and ursolic acid isomers in Ligustrum lucidum Ait.
abstract: In this paper, a novel method to separate and determine oleanolic acid and ursolic acid isomers in Ligustrum lucidum Ait was studied by micellar electrokinetic capillary chromatography (MECC). The baseline separation of the two analytes were obtained on the condition that the buffer contained 15 mmol/l disodium hydrogen phosphate, 15 mmol/l disodium tetreborate, 10 mmol/l SDS and 5% (v/v) alcohol. The contents of the oleanolic acid and ursolic acid were determined in L. lucidum Ait, Folium photiniae and Flos campsis; they were 78.3 (R.S.D. = 2.75%) and 20.7 mg/g (R.S.D. = 2.97%), 27.9 (R.S.D. = 3.67%) and 79.8 mg/g (R.S.D. = 3.44%), 65.5 (R.S.D. = 3.73%) and 60.4 mg/g (R.S.D. = 4.06%) (n = 5), respectively. The recoveries of the analytes in the extract of L. lucidum Ait were 102%, (R.S.D. = 2.85%) for oleanolic acid and 104% (R.S.D. = 3.21%) for ursolic acid (n = 5). With the emphasis on the effects of SDS and alcohol concentrations on the separation of the isomers were investigated.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130023, PR China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Liu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shi
authors.forename: Dexian
authors.initials: D
authors.lastname: Wang
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary; D007202:Indicators and Reagents; D031659:Ligustrum / Q000737:chemistry*; D009828:Oleanolic Acid / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
chemical_list: D007202:Indicators and Reagents; D009828:Oleanolic Acid; D010936:Plant Extracts; D012967:Sodium Dodecyl Sulfate; D014315:Triterpenes; D000097245:Ursolic Acid