Isolation and characterization of mutants of the Bacillus subtilis oligopeptide permease with altered specificity of oligopeptide transport.
Bacterial oligopeptide permeases are members of the large family of ATP binding cassette transporters and typically import peptides of 3 to 5 amino acids, apparently independently of sequence. Oligopeptide permeases are needed for bacteria to utilize peptides as nutrient sources and are sometimes involved in signal transduction pathways. The Bacillus subtilis oligopeptide permease stimulates competence development and the initiation of sporulation, at least in part, by importing specific signaling peptides. We isolated rare, partly functional mutations in B. subtilis opp. The mutants were resistant to a toxic tripeptide but still retained the ability to sporulate and/or become competent. The mutations, mostly in the oligopeptide binding protein located on the cell surface, affected residues whose alteration appears to change the specificity of oligopeptide transport.
Solomon
Jonathan
J
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. adg@mit.edu
Su
Laura
L
Shyn
Stanley
S
Grossman
Alan D
AD
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D008074:Lipoproteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D009154:Mutation; D009842:Oligopeptides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011485:Protein Binding; D021381:Protein Transport; D012097:Repressor Proteins / Q000494:pharmacology; D013045:Species Specificity; D013171:Spores, Bacterial / Q000201:enzymology
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D009842:Oligopeptides; D009943:Organophosphorus Compounds; C101115:PhrC protein, Bacillus subtilis; D012097:Repressor Proteins; C046506:oligopeptide-binding protein, bacteria; C003122:bialaphos; C037230:oligopeptide permease, Bacteria