Relationship between apoptosis and E-cadherin expression in bronchial epithelium of smoking mouse.
To investigate whether apoptosis is associated with cell adhesion in bronchial epithelium, and whether it contributes to the kinetics of injury and repair of surface epithelia, this study was performed for E-cadherin expression by using immunohistochemistry technique and for apoptosis by TUNEL method. An animal model of smoking was used for this study. The results showed that epithelial cells with membrane anchored E-cadherin decreased remarkably at several time points during 6 months of exposure to smoke (P < 0.01) and then restored to normal level. This fluctuation was associated exclusively with the alteration in number of apoptotic cells (P < 0.01). There was no significant difference in activation of nuclear transcription factor NF-kappa B among groups (P > 0.05). All these suggested that apoptosis is associated with E-cadherin expression in bronchial epithelium of smoking mouse.
Liu
Lifang
L
Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Yuan
Yonghui
Y
Li
Fang
F
Liu
Hongyun
H
D000818:Animals; D017209:Apoptosis; D001980:Bronchi / Q000378:metabolism / Q000473:pathology*; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D051379:Mice; D012907:Smoking / Q000009:adverse effects*
D015820:Cadherins