title: Simultaneous determination of methotrexate and its eight metabolites in human whole blood by capillary zone electrophoresis.
abstract: A simple and selective capillary electrophoretic method was established for the simultaneous determination of methotrexate (MTX), 7-hydroxymethotrexate (7-OHMTX), 2,4-diamino-N10-methylpteroic acid (DAMPA), and polyglutamate derivatives [MTX-(Glu)n, n=2-7] in whole blood. After extraction, those analytes were separated by fused-silica capillary and a running buffer containing glycine (1.2 M, pH 9.3). The quantitative ranges were 10-50 microM for each analyte. The intra- and inter-day RSD and RE values were all less than 6 and 11%, respectively. The limits of detection (S/N= 3, injection 5 s) were found to be 1 microM for MTX, 7-OHMTX, MTX-(Glu)2, MTX-(Glu)3, and MTX-(Glu)4; 3 microM for MTX-(Glu)5 and MTX-(Glu)6; 5 microM for MTX-(Glu)7, and 8 microM for DAMPA. All recoveries were greater than 94%. This method was applied to blood MTX monitoring in a patient with acute lymphoblastic leukemia.
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 100 Shih Chen 1st RD, Kaohsiung, 807 Taiwan, ROC.
authors.forename: Chien-Yuan
authors.initials: CY
authors.lastname: Kuo
authors.forename: Hsin-Lung
authors.initials: HL
authors.lastname: Wu
authors.forename: Hwang-Shang
authors.initials: HS
authors.lastname: Kou
authors.forename: Shyh-Shin
authors.initials: SS
authors.lastname: Chiou
authors.forename: Deng-Chyang
authors.initials: DC
authors.lastname: Wu
authors.forename: Shou-Mei
authors.initials: SM
authors.lastname: Wu
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005493:Folic Acid Antagonists / Q000097:blood*; D006801:Humans; D008727:Methotrexate / Q000097:blood*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000964:Antimetabolites, Antineoplastic; D005493:Folic Acid Antagonists; D008727:Methotrexate