"Whole cell"--matrix-assisted laser desorption ionization-time of flight-mass spectrometry, an emerging technique for efficient screening of biocombinatorial libraries of natural compounds-present state of research.
Whole Cell-matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is an emerging sensitive technique for rapid typing of microorganisms, efficient screening of biocombinatorial libraries of natural compounds and the analysis of complex biological samples, as whole cells, subcellular particles, cell extracts and culture filtrates. It is unique to detect metabolites in-situ without the need to isolate and purify the investigated compounds. In favourite cases it enables in-situ structure analysis on the basis of the fragment pattern generated by postsource MALDI-TOF-mass spectrometry. The state of research of this methodology which has mainly been obtained by investigation of lipopeptides from bacilli and the large spectrum of bioactive peptides produced by cyanobacteria is reviewed. The potential of this innovative technique is demonstrated for the lipopeptides produced by various Bacillus subtilis strains.
Vater
Joachim
J
Institut für Chemie, Arbeitsgruppe Biochemie und Molekulare Biologie, Technische, Universität Berlin, Franklinstr. 29, D-10587 Berlin, Germany. Vater@chem.tu-berlin.de
Gao
Xuewen
X
Hitzeroth
Gabriele
G
Wilde
Christopher
C
Franke
Peter
P
D001412:Bacillus subtilis / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D019151:Peptide Library; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D019151:Peptide Library