Evaluation of capillary HPLC/mass spectrometry as an alternative analysis method for gel electrophoresis of oligonucleotides.
A method has been developed to monitor the enzymatic incorporation of nucleotides in DNA by electrospray HPLC mass spectrometry. The main advantages of mass spectrometry over electrophoresis are the ability to directly characterize the reaction products and the shorter analysis time.
Rozenski
Jef
J
Rega Institute for Medical Research, K.U. Leuven, Leuven, Belgium. jef.rozenski@rega.kuleuven.ac.be
Vastmans
Karen
K
Van Aerschot
Arthur
A
Herdewijn
Piet
P
D001483:Base Sequence; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013698:Templates, Genetic
D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D004247:DNA