Glucose and galactose regulate intestinal absorption of cholesterol.
A dose-dependent increase in cholesterol absorption was induced by glucose addition (0-75 mM) to the apical medium of TC7 cells, a well-characterized clone of Caco-2. The uptake into the cells and the secretion rate to the basolateral space were both enhanced by glucose and galactose. This up-regulation was suppressed by SGLT1 inhibition but not by GLUT2 inhibition. Cholesterol cell uptake was significantly decreased by PMA and increased by chelerythrine, with more pronounced changes in the presence of hexoses. Thus, the involvement of a protein kinase C signalling pathway was evidenced in the regulation processes of intestinal cholesterol absorption. In the presence of antibodies directed to hSR-BI cholesterol absorption was reduced by 40% and glucose or galactose no longer enhanced it. We suggest that glucose or galactose, through an interaction with SGLT1, activates a protein kinase C pathway that regulates the activity of one of the intestinal cholesterol transporters, namely hSR-BI.
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Barbara
B
UMR-U 476--INSERM (Human Nutrition and Lipids, National Institute for Health and Medical Research), Faculté de Médecine, Université de la Méditerrannée, 27 Bd Jean Moulin, 13385 Marseille cedex 05, France.
Salvini
Séverine
S
Haikal
Ziad
Z
Charbonnier
Monique
M
Harbis
Amandine
A
Roussel
Magali
M
Lairon
Denis
D
Jourdheuil-Rahmani
Dominique
D
D001692:Biological Transport / Q000187:drug effects; D018955:CD36 Antigens / Q000502:physiology; D018938:Caco-2 Cells; D002784:Cholesterol / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005690:Galactose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007408:Intestinal Absorption; D026901:Membrane Transport Proteins / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein / Q000502:physiology; D051116:Receptors, Scavenger
D018955:CD36 Antigens; D026901:Membrane Transport Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D002784:Cholesterol; D011493:Protein Kinase C; D005947:Glucose; D005690:Galactose