title: Antisense phosphorothioate oligodeoxyribonucleotide targeted against ICAM-1: synthetic and biological characterization of a process-related impurity formed during oligonucleotide synthesis.
abstract: A phosphorothioate-linked oligonucleotide bearing a 3'-terminal phosphorothioate monoester has been synthesized and characterized. This oligonucleotide has been identified as a process-related impurity formed during synthesis of ISIS 2302. Biological properties of the compound have been determined. Based on these data, it can be concluded that this species (3'-TPT) has biological properties similar to parent drug.
authors.affiliation: Isis Pharmaceuticals, Inc., 2292 Faraday Avenue, Carlsbad, CA 92008, USA. vravikumar@isiph.com
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Capaldi
authors.forename: Walt F
authors.initials: WF
authors.lastname: Lima
authors.forename: Elena
authors.initials: E
authors.lastname: Lesnik
authors.forename: Brett
authors.initials: B
authors.lastname: Turney
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Cole
mesh_terms: D000818:Animals; D020811:Biomedical Technology; D016503:Drug Delivery Systems; D008401:Gas Chromatography-Mass Spectrometry; D007166:Immunosuppressive Agents / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D054735:Phosphorothioate Oligonucleotides; D011786:Quality Control; D016914:Ribonuclease H / Q000502:physiology; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D007166:Immunosuppressive Agents; D020319:Oligodeoxyribonucleotides, Antisense; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D054735:Phosphorothioate Oligonucleotides; D013873:Thionucleotides; D018799:Intercellular Adhesion Molecule-1; D016914:Ribonuclease H; C089753:alicaforsen