title: Role for Fes/Fps tyrosine kinase in microtubule nucleation through is Fes/CIP4 homology domain.
abstract: We have previously demonstrated that Fes/Fps (Fes) tyrosine kinase is involved in Semaphorin3A-mediated signaling. Here we report a role for Fes tyrosine kinase in microtubule dynamics. A fibrous formation of Fes was observed in a kinase-dependent manner, which associated with microtubules and functionally correlated with microtubule bundling. Microtubule regeneration assays revealed that Fes aggregates colocalized with gamma-tubulin at microtubule nucleation sites in a Fes/CIP4 homology (FCH) domain-dependent manner and that expression of FCH domain-deleted Fes mutants blocked normal centrosome formation. In support of these observations, mouse embryonic fibroblasts derived from Fes-deficient mice displayed an aberrant structure of nucleation and centrosome with unbundling and disoriented filaments of microtubules. Our findings suggest that Fes plays a critical role in microtubule dynamics including microtubule nucleation and bundling through its FCH domain.
authors.affiliation: Department of Genome Sciences, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Shusuke
authors.initials: S
authors.lastname: Takahashi
authors.forename: Ryoko
authors.initials: R
authors.lastname: Inatome
authors.forename: Azusa
authors.initials: A
authors.lastname: Hotta
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Qin
authors.forename: Renee
authors.initials: R
authors.lastname: Hackenmiller
authors.forename: M Celeste
authors.initials: MC
authors.lastname: Simon
authors.forename: Hirohei
authors.initials: H
authors.lastname: Yamamura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yanagi
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017931:DNA Primers; D015681:Fusion Proteins, gag-onc / Q000502:physiology*; D051379:Mice; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D011505:Protein-Tyrosine Kinases / Q000502:physiology*
chemical_list: D017931:DNA Primers; D015681:Fusion Proteins, gag-onc; D011505:Protein-Tyrosine Kinases; C068415:v-fps oncogene protein, Fujinami sarcoma virus