Adenoviral vector gene delivery via the round window membrane in guinea pigs.
We have found that damage from a local anesthetic solution containing phenol permitted beta-galactosidase (beta-gal) gene delivery to the guinea pig inner ear via the round window membrane (RWM). RWM damage was evident as degeneration of the outer epithelium. After adenovirus lacZ vector was applied to the damaged RWM, immunohistochemistry showed strong beta-gal expression in the RWM, mesothelial cells, organ of Corti, spiral limbus, spiral ligament and spiral ganglion. In the vestibular labyrinth, expression was seen in the sensory and supporting cells, transitional cells, and the dark-cell area. Thus, adenovirus can transfect a variety of inner ear cells in the guinea pig through a damaged RWM.
Suzuki
Mitsuya
M
Department of Otolaryngology, Tokyo Metropolitan Police Hospital, Tokyo, Japan. misuzuki-tky@umin.ac.jp
Yamasoba
Tatsuya
T
Suzukawa
Keigo
K
Kaga
Kimitaka
K
D000256:Adenoviridae / Q000235:genetics*; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D007758:Ear, Inner / Q000821:virology; D004848:Epithelium / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006168:Guinea Pigs; D016612:Paraffin Embedding; D012405:Round Window, Ear / Q000187:drug effects; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D000779:Anesthetics, Local; D001616:beta-Galactosidase