title: Modification of Ti6AL4V surfaces using collagen I, III, and fibronectin. II. Influence on osteoblast responses.
abstract: Responses of osteoblastic cells are influenced by morphology and composition of the extracellular matrix, and this fact has been used to improve the biological acceptance of implants by modifying the surfaces with components of the extracellular matrix (ECM). In this study, the effect of the collagen types I and III on adhesion, proliferation, and differentiation was studied, using primary osteoblastic cells from rat calvariae. Differences in alkaline phosphatase activity (ALP) and collagen synthesis were observed between differently composed collagen coatings. An increase in collagen type III resulted in an increase in collagen synthesis and a concomitant decrease in ALP activity and Ca deposition. Initial adhesion mechanism of the cells depended on the substrate (titanium, collagen, fibronectin).
authors.affiliation: Institute of Materials Science, Dresden University of Technology, Dresden, Germany. Susanne.bierbaum@mailbox.tu-dresden.de
authors.forename: Susanne
authors.initials: S
authors.lastname: Bierbaum
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Ulrike
authors.initials: U
authors.lastname: Geissler
authors.forename: Thomas
authors.initials: T
authors.lastname: Hanke
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Klaus-Wolfgang
authors.initials: KW
authors.lastname: Wenzel
authors.forename: Hartmut
authors.initials: H
authors.lastname: Worch
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry; D000818:Animals; D024042:Collagen Type I / Q000737:chemistry*; D024061:Collagen Type III / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D010006:Osteoblasts / Q000502:physiology*; D020836:Protein Structure, Quaternary; D051381:Rats; D012886:Skull / Q000502:physiology; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
chemical_list: D000497:Alloys; D024042:Collagen Type I; D024061:Collagen Type III; D005353:Fibronectins; D000535:Aluminum; D014025:Titanium