Over-expression of corticotropin-releasing factor mRNA in inferior olivary neurons of rolling mouse Nagoya.
Expression of corticotropin-releasing factor (CRF) mRNA was examined in the inferior olivary nucleus (ION) of an ataxic mutant, rolling mouse Nagoya (RMN) by semi-quantitative in situ hybridization. The most marked difference in the level of CRF mRNA signals between RMN and non-ataxic littermates (control mice) was observed in the beta-subnucleus and ventrolateral protrusion of the ION. The level of signals in these subnuclei was about twofold higher in RMN than in the controls. Signal levels in the dorsal nucleus, principal nucleus and subnucleus A were slightly but significantly higher in RMN than in the controls. In the other subnuclei, there were no differences in signal level between RMN and controls. These results suggest a region-related over-expression of CRF mRNA in the ION of RMN. This may be responsible for the increased sensitivity of some Purkinje cells to glutamate, resulting in ataxic symptoms of RMN.
Sawada
Kazuhiko
K
Department of Anatomy and Developmental Neurobiology, University of Tokushima School of Medicine, Tokushima 770-8503, Japan. sawada@basic.med.tokushima-u.ac.jp
Kawano
Michihiro
M
Tsuji
Hiroshi
H
Sakata-Haga
Hiromi
H
Hisano
Setsuji
S
Fukui
Yoshihiro
Y
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008818:Mice, Neurologic Mutants; D009474:Neurons / Q000378:metabolism*; D009847:Olivary Nucleus / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone