Increases in weight of growth hormone-deficient and immunodeficient (lit/scid) dwarf mice after grafting of hGH-secreting, primary human keratinocytes.
Primary human keratinocytes, stably transduced with the human growth hormone (hGH) gene (under control of the retroviral LTR promoter) and selected via geneticin secreted as much as 7 microg hGH/106 cells/day. Their grafting onto immunodeficient dwarf mice (lit/scid) led to hGH levels in the circulation that did not go below 0.2-0.3 ng/ml during a 12 day period (peak value, 1.5 ng/ml at 4 h). This phenomenon was associated with a body weight increase of the grafted animals (0.060 g/animal/day) significantly higher (P<0.01) than that of controls (0.023 g/animal/day). This is the first report describing successful utilization of immunodeficient dwarf mice (lit/scid) in keratinocyte-based hGH gene therapy.
Bellini
Maria Helena
MH
Biotechnology Department, IPEN-CNEN, University of São Paulo, Brazil.
Peroni
Cibele Nunes
CN
Bartolini
Paolo
P
D000818:Animals; D002478:Cells, Cultured; D005822:Genetic Vectors; D019382:Human Growth Hormone / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000637:transplantation*; D051379:Mice; D008817:Mice, Mutant Strains; D016513:Mice, SCID; D008954:Models, Biological; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic; D015430:Weight Gain
D019382:Human Growth Hormone