Mapping of 53 loci in American mink (Mustela vison).
Fifty-three genes were mapped in the American mink genome using polymerase chain reaction (PCR)-based analysis of a Chinese hamster-American mink somatic cell hybrid panel. Heterologous primers designed for cat gene mapping were used in this study. Forty-nine of these loci were localized into expected chromosome regions according to Zoo-FISH data, whereas four loci--ALPL, CDC20, ERF-2, and Fc(Mv)23617--were mapped out of expected conserved regions. PCR products amplified with primers corresponding to these four markers were partly sequenced and verified using BLAST. The results showed the homology to be more than 90% between mink and human or cat counterparts. At present, the gene map of American mink has expanded to 127 loci.
Kuznetsov
S B
SB
Laboratory of Developmental Genetics, Institute of Cytology and Genetics, Novosibirsk-90, Russia.
Matveeva
N M
NM
Murphy
W J
WJ
O'Brien
S J
SJ
Serov
O L
OL
D000818:Animals; D002874:Chromosome Mapping; D020223:Chromosome Painting; D017931:DNA Primers; D005819:Genetic Markers; D008907:Mink / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005819:Genetic Markers