Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces.
Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively.
Matsuura
Hiroshi
H
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Okamoto
Susumu
S
Anamnart
Sarintip
S
Wang
Qiuqi
Q
Zhou
Ze-Yang
ZY
Nihira
Takuya
T
Yamada
Yasuhiro
Y
Kuzuyama
Tomohisa
T
Seto
Haruo
H
Nakayama
Jiro
J
Suzuki
Akinori
A
Nagasawa
Hiromichi
H
Sakuda
Shohei
S
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014312:Trisaccharides / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D011994:Recombinant Proteins; D014312:Trisaccharides; C054851:allosamidin; D002688:Chitinases; D000117:Acetylglucosamine