Improved functional properties of the ovoinhibitor by conjugating with galactomannan.
The chicken egg white ovoinhibitor, a multi-type proteinase inhibitor, was conjugated with galactomannan through the Maillard reaction in a controlled dry heating state at 60 degrees C and 65% relative humidity. The formation of an ovoinhibitor-galactomannan conjugate during dry heating was confirmed by SDS-PAGE. The resulting ovoinhibitor-galactomannan conjugate showed almost the same inhibitory activity toward trypsin, chymotrypsin and elastase as that of the untreated ovoinhibitor, while the conjugate showed stronger heat stability and better emulsifying properties than the untreated ovoinhibitor. These results suggest that the ovoinhibitor-galactomannan conjugate can be used as a protease inhibitor having heat stability and outstanding emulsifying properties for industrial application.
Begum
Shamima
S
Department of Biological Chemistry, Yamaguchi University, Japan.
Saito
Akira
A
Xu
Xianhua
X
Kato
Akio
A
D000818:Animals; D002645:Chickens; D002918:Chymotrypsin / Q000378:metabolism; D004355:Drug Stability; D015387:Egg Proteins, Dietary / Q000494:pharmacology*; D004529:Egg White; D004591:Electrophoresis, Polyacrylamide Gel; D045703:Emulsifying Agents / Q000737:chemistry / Q000494:pharmacology; D005690:Galactose / Q000031:analogs & derivatives; D006358:Hot Temperature; D015416:Maillard Reaction; D008351:Mannans / Q000737:chemistry* / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
D015387:Egg Proteins, Dietary; D045703:Emulsifying Agents; D008351:Mannans; D015842:Serine Proteinase Inhibitors; C012990:galactomannan; C004224:ovoinhibitor; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D005690:Galactose