A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445.
We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.
Iida
Reiko
R
Department of Forensic Medicine, Fukui Medical University, Fukui 910-1193, Japan. ireiko@fmsrsa.fukui-med.ac.jp
Sawazaki
Kazumi
K
Ikeda
Hitoe
H
Miyamoto
Taito
T
Tsubota
Etsuko
E
Takatsuka
Hisakazu
H
Masuyama
Mika
M
Matsuki
Takasumi
T
Yasuda
Toshihiro
T
Kishi
Koichiro
K
D001801:Blood Stains; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012661:Semen / Q000737:chemistry; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA