Nucleosome position-dependent and -independent activation of HIS7 epression in Saccharomyces cerevisiae by different transcriptional activators.
ARO4 and HIS7 are two tandemly orientated genes of Saccharomyces cerevisiae that are transcribed into the same direction. The ARO4 terminator and the HIS7 promoter regions are sensitive to Micrococcus nuclease (Mnase) and separated by a positioned nucleosome. The HIS7 promoter is target for the transcription factors Gcn4p and Bas1p/Bas2p that activate its transcription upon amino acid starvation and purine limitation, respectively. Activation of the HIS7 gene by Gcn4p overexpression but not by Bas1p/Bas2p releases an ordered nucleosome distribution to yield increased Mnase sensitivity throughout the intergenic region. This remodeling is SNF2 dependent but mostly GCN5 independent. Accordingly, SNF2 is necessary for the Gcn4p-mediated transcriptional activation of the HIS7 gene. GCN5 is required for activation upon adenine limitation by Bas1p/Bas2p. Our data suggest that activation of HIS7 transcription by Gcn4p and Bas1p/Bas2p is supported by a nucleosome position-dependent and -independent mechanism, respectively. Whereas Gcn4p activation causes Swi/Snf-mediated remodeling of the nucleosomal architecture at the HIS7 promoter, the Bas1p/Bas2p complex presumably activates in combination with Gcn5p-dependent histone acetylation.
Valerius
Oliver
O
Institute of Microbiology and Genetics, Georg-August-University, D-37077 Göttingen, Germany.
Brendel
Cornelia
C
Wagner
Claudia
C
Krappmann
Sven
S
Thoma
Fritz
F
Braus
Gerhard H
GH
D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000225:Adenine / Q000378:metabolism; D000251:Adenosine Triphosphatases; D000446:Aldehyde-Lyases / Q000235:genetics; D000619:Aminohydrolases; D015152:Blotting, Northern; D015139:Blotting, Southern; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021901:DNA, Intergenic / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D003851:Deoxyribonucleases / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005838:Genotype; D051548:Histone Acetyltransferases; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology; D008957:Models, Genetic; D009097:Multienzyme Complexes / Q000235:genetics*; D009687:Nuclear Proteins; D009707:Nucleosomes / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D015533:Transcriptional Activation; D014166:Transferases / Q000235:genetics*; D000099224:p300-CBP-Associated Factor
D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000225:Adenine; D000251:Adenosine Triphosphatases; D000446:Aldehyde-Lyases; D000619:Aminohydrolases; D002843:Chromatin; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D003851:Deoxyribonucleases; D051548:Histone Acetyltransferases; D018398:Homeodomain Proteins; D009097:Multienzyme Complexes; D009687:Nuclear Proteins; D009707:Nucleosomes; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D014166:Transferases; C061767:BAS1 protein, S cerevisiae; C051611:PHO2 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C087227:imidazole glycerol phosphate synthase; C499333:SNF2 protein, S cerevisiae