Novel base-labile protecting groups for 5'-hydroxy function in solid-phase oligonucleotide synthesis.
The 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMMoNBz) and 2-(levulinyloxymethyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz) groups were developed as novel base-labile protection for the 5'-hydroxy function in solid-phase oligonucleotide synthesis. A comparative study of the LMMoNBz, LMMpNBz and 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) protecting groups for oligonucleotide synthesis proved strong feasibility for the LMMoNBz group.
Kamaike
Kazuo
K
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan. kamaikek@ps.toyaku.ac.jp
Namiki
Takashi
T
Kawashima
Etsuko
E
D002851:Chromatography, High Pressure Liquid; D006900:Hydroxylation; D007202:Indicators and Reagents; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013941:Thymine
D007202:Indicators and Reagents; D009841:Oligonucleotides; D013941:Thymine