A novel bioartificial liver containing small tissue fragments: efficiency in the treatment of acute hepatic failure induced by carbon tetrachloride in rats.
The efficiency of a new bioartificial liver (BAL) containing small tissue fragments in the treatment of acute hepatic failure induced by carbon tetrachloride in rats was evaluated. A day after injection (i.p.) of CCl4 the animals were connected to a BAL containing liver fragments (fragment BAL) and a BAL containing no liver fragments (no-fragment BAL), and extracorporeal hemoperfusion was carried out for 4 h. The activities of alanine transaminase and lactate dehydrogenase as well as the concentrations of ammonia, glucose, urea, and amino acids in plasma were measured. A tendency to the stabilisation of ammonia, glucose, phenylalanine, tyrosine, and other amino acids was revealed at the end of hemoperfusion in poisoned rats connected to the fragment BAL. A statistically significant difference in survival between the animals connected to the fragment BAL and no-fragment BAL was found. The results obtained indicate that the bioreactor containing small liver fragments is effective in the treatment of acute hepatic failure in animals.
Soloviev
V
V
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia.
Hassan
A N E
AN
Akatov
V
V
Lezhnev
E
E
Ghaffar
T Y A
TY
Ghaffar
Y A
YA
D000596:Amino Acids / Q000097:blood; D000818:Animals; D019149:Bioreactors; D001774:Blood Chemical Analysis; D002251:Carbon Tetrachloride; D004867:Equipment Design; D017093:Liver Failure / Q000139:chemically induced / Q000628:therapy*; D019164:Liver, Artificial; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D002251:Carbon Tetrachloride