Conversion of adamsite (phenarsarzin chloride) by fungal manganese peroxidase.
Fungal manganese peroxidase was found to convert the persistent chemical warfare agent adamsite (phenarsarzin chloride) in a cell-free reaction mixture containing sodium malonate, Mn(2+) ions, and reduced glutathione. The organo-arsenical compound disappeared completely within 48 h accompanied by the formation of a more polar metabolite with a clearly modified UV spectrum. Thus, As(III) in the adamsite molecule was oxidized by manganese peroxidase to As(V) which added dioxygen and released chloride.
Haas
R
R
Office of Environmental Investigation and Research, Stadtwaldstrasse 45a, 35037 Marburg, Germany. haasr@gmx.net
Tsivunchyk
O
O
Steinbach
K
K
von Löw
E
E
Scheibner
K
K
Hofrichter
M
M
D001152:Arsenicals / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D002474:Cell-Free System; D002619:Chemical Warfare Agents / Q000378:metabolism; D005978:Glutathione; D008314:Malonates; D008345:Manganese; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*
D001152:Arsenicals; D002619:Chemical Warfare Agents; D008314:Malonates; D008345:Manganese; C030290:malonic acid; D010544:Peroxidases; C051129:manganese peroxidase; D005978:Glutathione; C010283:phenarsazine chloride