title: A negative regulatory element controls mRNA abundance of the Leishmania mexicana Paraflagellar rod gene PFR2.
abstract: The Leishmania mexicana PFR2 locus encodes a component of the paraflagellar rod (PFR), a flagellar structure found only in the insect stage of the life cycle. PFR2 mRNA levels are 10-fold lower in the mammalian stage than in the insect stage. Nuclear run-on experiments indicate that the change in PFR2 mRNA abundance is achieved posttranscriptionally. Deletion and block substitution analysis of the entire 1,400-nucleotide 3' untranslated region (UTR) of PFR2C led to the identification of a regulatory element contained within 10 nucleotides of the 3' UTR, termed the PFR regulatory element (PRE), that is necessary for the 10-fold regulation of PFR2 mRNA levels. Comparison of the half-lives of PFR2 transcripts, identical except for the presence or absence of the PRE, revealed that the PRE acts by destabilizing the PFR2 mRNA in amastigotes. The PRE was inserted into a construct which directs the constitutive expression of a chimeric PFR2 transcript. Insertion of the PRE resulted in regulated expression of this transcript, demonstrating that the regulatory element is sufficient for promastigote-specific expression. Since the PRE is present in the 3' UTR of all L. mexicana PFR genes examined so far, we propose that it serves a means of coordinating expression of PFR genes.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-2063, USA.
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Mishra
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Holzer
authors.forename: Landon L
authors.initials: LL
authors.lastname: Moore
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: LeBowitz
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003609:Dactinomycin / Q000494:pharmacology; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D006207:Half-Life; D007894:Leishmania mexicana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D014170:Transformation, Genetic
chemical_list: D020413:3' Untranslated Regions; C105582:PFR-2 protein, Leishmania; D015800:Protozoan Proteins; D012333:RNA, Messenger; D003609:Dactinomycin