title: Choristoneura fumiferana Granulovirus p74 protein, a highly conserved baculoviral envelope protein.
abstract: A gene that encodes a homologue to baculoviral p74, an envelope-associated viral structural protein, has been identified and sequenced on the genome of Choristoneura fumiferana granulovirus (ChfuGV). A part of the ChfuGV p74 gene was located on an 8.9 kb BamHI subgenomic fragment using different sets of degenerated primers. These were designed using the results of the protein sequencing of a major 74 kDa structural protein that is associated with the occlusion-derived virus (ODV). The gene has a 1992 nucleotide (nt) open-reading frame (ORF) that encodes a protein with 663 amino acids with a predicted molecular mass of 74,812 Da. Comparative studies revealed the presence of two major conserved regions in the ChfuGV p74 protein. This study also shows that all of the p74 proteins contain two putative transmembrane domains at their C-terminal segments. At the nucleotide sequence level, two late promoter motifs (TAAG and GTAAG) were located upstream of the first ATG of the p74 gene. The gene contained a canonical poly(A) signal, AATAAA, at its 3   non-translated region. A phylogenetic tree for baculoviral p74 was constructed using a maximum parsimony analysis. The phylogenetic estimation demonstrated that ChfuGV p74 is related the closest to those of Cydia pomonella granulovirus (CpGV) and Phthorimaea operculella granulovirus (PhopGV).
authors.affiliation: Institut national de la recherche scientifique, Institut Armand-Frappier, 531 Blvd des Prairies, Laval, Canada H7V 1B7. kiarashidan@juno.com
authors.forename: Kianoush Khajeh
authors.initials: KK
authors.lastname: Rashidan
authors.forename: Nasha
authors.initials: N
authors.lastname: Nassoury
authors.forename: Samia
authors.initials: S
authors.lastname: Tazi
authors.forename: Paresa N
authors.initials: PN
authors.lastname: Giannopoulos
authors.forename: Claude
authors.initials: C
authors.lastname: Guertin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D025722:Granulovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins