Influence of histamine in a liver injury model induced by Propionibacterium acnes and lipopolysaccharide.
In normal mice, plasma histamine levels were 29.4+/-10.1 pmol/ml. When 0.1 microg of lipopolysaccharide (LPS) was intravenously injected into Propionibacterium acnes (P. acnes)-primed ICR mice, histamine levels increased remarkably to 61.2+/-15.9 pmol/ml (p<0.001). An increase was also observed in liver tissues. Oral administration of histidine at 200 mg/kg once daily for 5 d before intravenous LPS injection increased the plasma alanine aminotransferase (ALT) activity to 2936.5+/-356.3 IU/l, a significant change compared with the controls (2244.8+/-425.5 IU/l, p<0.05). The 24 h survival rate after LPS injection was 72.7% in the mice treated with 50 mg/kg of ranitidine, in contrast with 50% in the control group although the treatment did not significantly decrease the plasma ALT activity. On the other hand, 50 mg/kg of pyrilamine significantly reduced plasma ALT activity (p<0.001). The results suggested that histamine levels are related to hepatic damage in the P. acnes plus LPS induction of liver injury.
Kurihara
Hiroshi
H
Institute of Traditional Chinese and Natural Medicine, Jinan University, China. Hiroshi_Kurihara@suntory.co.jp
Fukami
Harukazu
H
Asami
Sumio
S
Shibata
Hiroshi
H
Kiso
Yoshinobu
Y
Tanaka
Takaharu
T
Yao
Xin-Sheng
XS
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D004195:Disease Models, Animal; D004357:Drug Synergism; D006632:Histamine / Q000097:blood*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008107:Liver Diseases / Q000097:blood* / Q000382:microbiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011425:Propionibacterium acnes
D008070:Lipopolysaccharides; D006632:Histamine; D000410:Alanine Transaminase