title: Optimal timing for canine artificial insemination with frozen semen and parentage testing by microsatellite markers in superfecundency.
abstract: Parentage testing was performed in sixteen litters by canine artificial inseminations with frozen semen from different sires on Days 5 and 7 after the LH surge. It became apparent that only 25% of dams had superfecundation, but 43.8% of dams were whelped after insemination only on Day 5 after the LH surge and 31.3% of dams after insemination only on Day 7. Of the total 87 puppies, 46% were born after insemination on Day 5 after the LH surge and 54% after insemination on Day 7. This result strongly suggested that canine artificial insemination with frozen semen could be sufficiently successful also on Days 5 and 7 after the LH surge.
authors.affiliation: Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tsumagari
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toriumi
authors.forename: Kakashi
authors.initials: K
authors.lastname: Ishihama
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Morita
authors.forename: Misao
authors.initials: M
authors.lastname: Kanamaki
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Takeishi
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D004285:Dogs; D005260:Female; D005298:Fertility / Q000502:physiology*; D005819:Genetic Markers; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D008297:Male; D018895:Microsatellite Repeats; D012661:Semen / Q000502:physiology*
chemical_list: D005819:Genetic Markers; D007986:Luteinizing Hormone