[The intervention of reduced glutathione in neutrophil activation in the process of extracorporeal circulation].
OBJECTIVE
To study the intervention of reduced glutathione for neutrophil activation in the process of extracorporeal circulation.


METHODS
20 congenital heart disease children aged from 6 to 12 years were selected randomly, divided into control group and interventional group, patients of interventional group were added reduced glutathione (30 mg/kg) in priming fluid, then phlebotomized on the time of 24 hours before operation, 20 minutes after CPB, rewarming, the end of CPB, 4 hours after operation, 24 hours after operation. After separated neutrophil, we distilled nuclear protein and mensurated the activation of nuclear factor-kappaB by EMSA, assayed the expression of ICAM-1. by immunohistochemistry.


RESULTS
Neutrophil nuclear factor-kappaB has been activated on the time of 20 minutes after CPB, arrives at the top on the time of rewarming, and resumes 24 hours after operation; ICAM-1 expresses on the time of rewarming, arrives at the top 4 hours after operation in control group. The data of interventional group is lower than the control group.


CONCLUSIONS
Neutrophil nuclear factor-kappaB is activated and ICAM-1 is expressed in the process of extracorporeal circulation and play an important role in the inflammatory response happening in extracorporeal circulation, which could be relieved by reduced glutathione.
Ni
Hai-feng
HF
Department of Cardiac Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Xiao
Ying-bin
YB
D002315:Cardiopulmonary Bypass; D002648:Child; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D018375:Neutrophil Activation / Q000187:drug effects*
D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1; D005978:Glutathione