title: Enhancement of DNA micro-array analysis using a shear-driven micro-channel flow system.
abstract: A very simple micro-channel flow system is used to investigate the potential gain in hybridization rate stemming from the induction of a convective flow past the surface of a DNA micro-array. Reporting on a series of preliminary experiments wherein a two-dimensional convective flow is created past the surface of a conventional micro-array slide, the analysis time could be brought down from overnight waiting (16 h) to some 10 to 30 min. The experiments open the road towards the development of novel, convection-driven hybridization systems yielding shorter analysis times, and/or lower detection limits.
authors.affiliation: Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. kpappaer@vub.ac.be
authors.forename: K
authors.initials: K
authors.lastname: Pappaert
authors.forename: J
authors.initials: J
authors.lastname: Vanderhoeven
authors.forename: P
authors.initials: P
authors.lastname: Van Hummelen
authors.forename: B
authors.initials: B
authors.lastname: Dutta
authors.forename: D
authors.initials: D
authors.lastname: Clicq
authors.forename: G V
authors.initials: GV
authors.lastname: Baron
authors.forename: G
authors.initials: G
authors.lastname: Desmet
mesh_terms: D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity