In-vitro endothelialization of patches of bovine jugular vein stabilized by dye-mediated photooxidation.
OBJECTIVE
The study aim was to evaluate the cytotoxicity of photooxidatively stabilized bovine jugular vein and the in-vitro endothelialization properties of such material.


METHODS
Bovine jugular veins were cross-linked by dye-mediated photooxidation and cut into 4 cm2 pieces. Ovine jugular vein endothelial cells were isolated, cultured, and then seeded onto the inner surface of bovine jugular vein patches at a density of 1.5 x 10(5) per cm2. Specimens were cultured for five days and then examined using scanning electron microscopy (SEM) and immunohistochemical staining for von Willebrand factor.


RESULTS
SEM showed confluent lining of cultured ovine jugular vein endothelial cells similar to native endothelium. Von Willebrand factor staining for cells on the surface of patches was positive.


CONCLUSIONS
The photooxidatively stabilized bovine jugular vein patches were not cytotoxic, and their in-vitro endothelialization was possible.
Li
Shengli
S
Division of Cardiac Valvular Prosthesis and Circulation Assistance, Department of Surgery, Fuwai Heart Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, Peoples' Republic of China.
Hu
Shengshou
S
Zhou
Jianye
J
Liao
Zhongkai
Z
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004396:Coloring Agents; D042783:Endothelial Cells / Q000187:drug effects*; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D007601:Jugular Veins / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D008955:Models, Cardiovascular; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000032:analysis / Q000187:drug effects
D004396:Coloring Agents; D010083:Oxidants, Photochemical; D014841:von Willebrand Factor; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin