Elaboration of enzyme immunoassay based on recombinant antigens and intended for diagnostics of syphilis.
Enzyme immunoassay (EIA) using recombinant antigens for the detection of Treponema pallidum-specific antibodies in sera of syphilis patients was developed. Four low-molar-mass Treponema antigens (Tp15, Tp17, TmpA, Tp47) were investigated; 17- and 47-kDa proteins were demonstrated as immunodominant as they permitted to obtain the most sensitive EIA. Using a mixture of these proteins a 3rd-generation-EIA kit Dia-Syph was constructed, its sensitivity being 99.4% during tests of 165 sera of syphilitic patients. No false result was obtained on the commercial panel PSS01 (BBI, USA). The specificity of the elaborated test system (99.7%) was determined on 295 sera.
Rostopira
N
N
Joint Stock Company DiaProph Med, Kiev, Ukraine.
Tkáciková
L
L
Rayevska
G
G
Pylypenko
V
V
Mikula
I
I
Spivak
M
M
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013588:Syphilis Serodiagnosis; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011994:Recombinant Proteins