title: Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces.
abstract: Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively.
authors.affiliation: Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuura
authors.forename: Susumu
authors.initials: S
authors.lastname: Okamoto
authors.forename: Sarintip
authors.initials: S
authors.lastname: Anamnart
authors.forename: Qiuqi
authors.initials: Q
authors.lastname: Wang
authors.forename: Ze-Yang
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Takuya
authors.initials: T
authors.lastname: Nihira
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kuzuyama
authors.forename: Haruo
authors.initials: H
authors.lastname: Seto
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Akinori
authors.initials: A
authors.lastname: Suzuki
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Shohei
authors.initials: S
authors.lastname: Sakuda
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014312:Trisaccharides / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D011994:Recombinant Proteins; D014312:Trisaccharides; C054851:allosamidin; D002688:Chitinases; D000117:Acetylglucosamine