title: A population of c-Kit(low)(CD45/TER119)- hepatic cell progenitors of 11-day postcoitus mouse embryo liver reconstitutes cell-depleted liver organoids.
abstract: Embryo liver morphogenesis takes place after gastrulation and starts with a ventral foregut evagination that reacts to factor signaling from both cardiac mesoderm and septum transversum mesenchyme. Current knowledge of the progenitor stem cell populations involved in this early embryo liver development is scarce. We describe here a population of 11-day postcoitus c-Kit(low)(CD45/TER119)- liver progenitors that selectively expressed hepatospecific genes and proteins in vivo, was self-maintained in vitro by long-term proliferation, and simultaneously differentiated into functional hepatocytes and bile duct cells. Purified c-Kit(low)(CD45/TER119)- liver cells cocultured with cell-depleted fetal liver fragments engrafted and repopulated the hepatic cell compartments of the latter organoids, suggesting that they may include the embryonic stem cells responsible for liver development.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas-Universidad Autónoma de Madrid, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Minguet
authors.forename: Isabel
authors.initials: I
authors.lastname: Cortegano
authors.forename: Pilar
authors.initials: P
authors.lastname: Gonzalo
authors.forename: José-Alberto
authors.initials: JA
authors.lastname: Martínez-Marin
authors.forename: Belén
authors.initials: B
authors.lastname: de Andrés
authors.forename: Clara
authors.initials: C
authors.lastname: Salas
authors.forename: David
authors.initials: D
authors.lastname: Melero
authors.forename: Maria-Luisa
authors.initials: ML
authors.lastname: Gaspar
authors.forename: Miguel A R
authors.initials: MA
authors.lastname: Marcos
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000502:physiology*; D007633:Keratins / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D053683:Oncostatin M; D010455:Peptides / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*
chemical_list: C508352:Osm protein, mouse; D010455:Peptides; D012333:RNA, Messenger; D053683:Oncostatin M; D017228:Hepatocyte Growth Factor; D007633:Keratins; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens