title: Biologically inspired synthesis of bone-like composite: self-assembled collagen fibers/hydroxyapatite nanocrystals.
abstract: Replacement of bone tissue by graft materials and products of tissue engineering having composition, structure, and biological features that mimic natural tissue is a goal to be pursued. A biomimetic synthesis was performed to prepare new bone-like composites constituted of hydroxyapatite nanocrystals and self-assembled type I collagen fibers. We used a biological inspired approach that proved that the biological systems stored and processed information at the molecular level. Two different methodologies were used: dispersion of synthetic hydroxyapatite in telopeptides free collagen molecules solution and direct nucleation of hydroxyapatite into reconstituted collagen fibers during their assembling. The different preparation techniques were experimented then the composites thoroughly characterized and compared. Composite obtained by direct nucleation showed an intimated interaction of the inorganic and proteic components, which modified the apatitic phase and made its composition, morphology and structure similar to the mineral component of natural bone.
authors.affiliation: Institute of Science and Technology for Ceramic-CNR, via Granarolo 64, I-48018 Faenza (RA), Italy. tampieri@istec.cnr.it
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Celotti
authors.forename: Elena
authors.initials: E
authors.lastname: Landi
authors.forename: Monica
authors.initials: M
authors.lastname: Sandri
authors.forename: Norberto
authors.initials: N
authors.lastname: Roveri
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Falini
mesh_terms: D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D017886:Durapatite / Q000737:chemistry*; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite