title: Analysis of the transcriptional response to Rice Yellow Mottle Virus infection in Oryza sativa indica and japonica cultivars.
abstract: Several cDNA libraries were constructed using mRNA isolated from roots, panicles, cell suspensions and leaves of non-stressed Oryza sativa indica (IR64) and japonica (Azucena) plants, from wounded leaves, and from leaves of both cultivars inoculated with Rice Yellow Mottle Virus (RYMV). A total of 5549 cleaned expressed sequence tags (ESTs) were generated from these libraries. They were classified into functional categories on the basis of homology, and analyzed for redundancy within each library. The expression profiles represented by each library revealed great differences between indica and japonica backgrounds. EST frequencies during the early stages of RYMV infection indicated that changes in the expression of genes involved in energy metabolism and photosynthesis are differentially accentuated in susceptible and partially resistant cultivars. Mapping of these ESTs revealed that several co-localize with previously described resistance gene analogs and QTLs (quantitative trait loci).
authors.affiliation: UMR 5096, Institut de Recherche pour le Développement, BP 64501, 34394 Montpellier, France. ventelon@mpl.ird.fr
authors.forename: M
authors.initials: M
authors.lastname: Ventelon-Debout
authors.forename: T-T-H
authors.initials: TT
authors.lastname: Nguyen
authors.forename: A
authors.initials: A
authors.lastname: Wissocq
authors.forename: C
authors.initials: C
authors.lastname: Berger
authors.forename: M
authors.initials: M
authors.lastname: Laudie
authors.forename: B
authors.initials: B
authors.lastname: Piégu
authors.forename: R
authors.initials: R
authors.lastname: Cooke
authors.forename: A
authors.initials: A
authors.lastname: Ghesquière
authors.forename: M
authors.initials: M
authors.lastname: Delseny
authors.forename: C
authors.initials: C
authors.lastname: Brugidou
mesh_terms: D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D015723:Gene Library; D005819:Genetic Markers; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D040641:Quantitative Trait Loci; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers