Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis.
Two microsatellites were isolated from a genomic library of Echinococcus multilocularis. The microsatellites, designated EMms1 and EMms2, consist of tandem repeats of CAC-trinucleotide unit. Southern blot hybridization suggests that each of them is a single locus. Using fox-derived wild tapeworms (N=104), PCR-amplification of microsatellites was performed to assess the usefulness of these loci. We found four alleles of EMms1 and two alleles of EMms2. The heterozygosities observed were 10.6% in EMms1 and 7.7% in EMms2. The result suggests that both selfing and outcrossing occur in the adult stage of E. multilocularis.
Nakao
Minoru
M
Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078-8510, Japan. nakao@asahikawa-med.ac.jp
Sako
Yasuhito
Y
Ito
Akira
A
D000818:Animals; D004446:Echinococcus / Q000235:genetics*; D005589:Foxes / Q000469:parasitology; D015723:Gene Library; D006579:Heterozygote; D007564:Japan; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic