title: Calmodulin gene expression in the neural retina of the adult rat.
abstract: Calmodulin (CaM) mRNAs are expressed with low abundancy in the adult rat neural retina. However, when digoxigenin (DIG)-labeled cRNA probes specific for each CaM mRNA population were hybridized at slightly alkaline pH (pH 8.0), the widespread distribution of CaM mRNA-expressing cells was revealed, with similar abundance for all three CaM genes. The CaM genes displayed a uniquely similar, layer-specific expression throughout the retina, and no significant differences were found in the distribution patterns of the CaM mRNA populations or the labeled cell types. The strongest signal for all CaM mRNAs was demonstrated in the ganglion cell layer and the inner nuclear layer, where the highest signal intensity was found within the inner sublamina. Similarly intermediate signal intensities for all CaM genes were detected in the inner and outer plexiform layers, within the vicinity of the outer limiting membrane and in the retinal pigment epithelium. A very low specific signal was characteristic in the outer nuclear layer and the photoreceptor inner segment layer, while no specific hybridization signal was observed in the photoreceptor outer segment layer. In summary, all CaM genes exhibited a similar and a characteristically layer-specific expression pattern in the adult rat retina.
authors.affiliation: Department of Zoology and Cell Biology, University of Szeged, 2 Egyetem u., POB 659, Szeged, H-6722, Hungary.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Kovacs
authors.forename: Karoly
authors.initials: K
authors.lastname: Gulya
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000235:genetics* / Q000378:metabolism; D003116:Color; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D012333:RNA, Messenger