High-performance capillary electrophoresis with indirect UV detection for determination of alpha-galactosides in Leguminosae and Brassicaceae.
A rapid, easy, and reproducible capillary electrophoresis method for determination of raffinose family oligosaccharides (alpha-galactosides) was developed. Sucrose, raffinose, stachyose, verbascose, and ajugose were determined with indirect UV detection at moderate alkaline pH 9.2, using pyridine-2,6-dicarboxylic acid as background electrolyte in a sodium tetraborate buffer with added cetyltrimethylammonium bromide. The separation efficiency measured by the number of theoretical plates (N) ranged from 1.4 x 10(5) to 2.3 x 10(5). The precision of the method, measured by the relative standard deviation (RSD), was less than 0.53% for the migration times and better than 3.4% for normalized areas (NA), considering all sugars except verbascose (RSD(NA) = 11.8%). Detection limits were about 110 microg/mL, corresponding to 150-320 microM. Relative response factors (RRF) were calculated on the basis of linearity studies and used for quantification of alpha-galactosides in a lupine sample (Lupinus angustifolius).
Andersen
Keld E
KE
Chemistry Department, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Bjergegaard
Charlotte
C
Møller
Peter
P
Sørensen
Jens C
JC
Sørensen
Hilmer
H
D019607:Brassicaceae / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005697:Galactosides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D031297:Lupinus / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis; D011887:Raffinose / Q000032:analysis; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000032:analysis; D014466:Ultraviolet Rays
D005697:Galactosides; D009844:Oligosaccharides; C005695:stachyose; D013395:Sucrose; D011887:Raffinose