Experimental study of vascular endothelial growth factor gene therapy for avascular necrosis of the femoral head.
To explore a new method for the therapy of the avascular necrosis of the femoral head, the recombinant plasmid pCD-hVEGF165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly.
Yang
Cao
C
Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Yang
Shuhua
S
Du
Jingyuan
J
Li
Jin
J
Xu
Weihua
W
Xiong
Yufang
Y
D000818:Animals; D003094:Collagen / Q000627:therapeutic use; D005270:Femur Head / Q000098:blood supply*; D005271:Femur Head Necrosis / Q000628:therapy*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D020007:Implants, Experimental; D018919:Neovascularization, Physiologic; D010012:Osteogenesis; D010957:Plasmids / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics*
D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D003094:Collagen