Differential expression of transforming growth factors-beta 1, -beta 2, -beta 3 and the type I, II, III receptors in the lining epithelia of inflamed gingiva.
OBJECTIVE
To investigate the distribution of transforming growth factor-beta isoforms in chronically inflamed periodontal tissues.


METHODS
The present study determined, by immunohistochemistry, the expression patterns of TGF-betas and their receptors in the lining epithelia of inflamed gingiva. Frozen sections were obtained from 22 human gingival biopsies.


RESULTS
TGF-beta 1 was not detected in gingival epithelial cells in examined sections. Detection of TGF-beta 2 indicated a progressive reduction of staining from the external oral epithelium through to gingival sulcus and the gingival attachment or pocket epithelium. TGF-beta 3 showed intense staining in all domains of both minimally inflamed gingiva and advanced periodontitis tissues. TGF-beta RI was visualised as focal staining of the spinous layer in the external oral epithelium of both periodontitis lesions and minimally inflamed tissues. TGF-beta RII was present throughout the strata, but with progressive reduction in intensity from the oral epithelium to gingival attachment or pocket epithelium respectively while, conversely, TGF-beta RIII showed an increase in diffuse staining intensity from external oral epithelium to pocket epithelium.


CONCLUSIONS
A distinct expression profile was observed within different individuals for TGF-betas and the corresponding receptors. These findings provide a basis for evaluation of the role of these growth factors in the pathogenesis of periodontitis.
Ye
Ping
P
Institute of Dental Research, Westmead Centre for Oral Health, Westmead, NSW, Australia.
Simonian
Mary
M
Chapple
Cheryl C
CC
Gibbins
John R
JR
Kumar
Rakesh K
RK
Hunter
Neil
N
D015153:Blotting, Western; D002908:Chronic Disease; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D005891:Gingivitis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010518:Periodontitis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta