High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction.
A HPLC method was developed for the analysis of secoisolariciresinol diglucoside (SDG) and hydroxycinnamic acid glucosides in milled defatted flaxseed flour. Direct extraction by 1 M NaOH for 1 h at 20 degrees C resulted in a higher yield than that obtained by hydrolysis of alcoholic extracts. An internal standard, o-coumaric acid, was used and the method was found to be easy, fast, and with good repeatability. On dry matter basis, different samples of flaxseeds varied considerably in their content of (+)-SDG (11.9-25.9 mg/g), (-)-SDG (2.2-5.0 mg/g), p-coumaric acid glucoside (1.2-8.5 mg/g), and ferulic acid glucoside (1.6-5.0 mg/g).
Eliasson
Christina
C
Department of Food Science, Swedish University of Agricultural Sciences (SLU), P.O. Box 7051, SE-750 07 Uppsala, Sweden. christina.eliasson@lmv.slu.se
Kamal-Eldin
Afaf
A
Andersson
Roger
R
Aman
Per
P
D000468:Alkalies / Q000737:chemistry; D002072:Butylene Glycols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D019597:Flax / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D006868:Hydrolysis; D017705:Lignans; D012015:Reference Standards; D015203:Reproducibility of Results
D000468:Alkalies; D002072:Butylene Glycols; D003373:Coumaric Acids; D006027:Glycosides; D017705:Lignans; C060283:secoisolariciresinol