title: AmpFlSTR profiler Plus short tandem repeat DNA analysis of casework samples, mixture samples, and nonhuman DNA samples amplified under reduced PCR volume conditions (25 microL).
abstract: As part of the validation of the AmpFlSTR Profiler Plus short tandem repeat (STR) system, under reduced polymerase chain reaction (PCR) volume conditions (i.e., 25 microL), a total of 275 casework samples were processed. Examples of profiles are presented along with amplification conditions to improve the odds of obtaining balanced and complete profiles for samples showing partial results or profiles with a descending slope. Data collected and used to develop our interpretation guidelines are included. From the mixture studies, full profiles were obtained for minor contributors, using 2 ng of DNA, with ratios of 10:1 or 1:20 and using 1 ng of DNA, with ratios of 10:1 and 1:8. The specificity of the Profiler Plus amplification reaction performed in 25 microL was examined and confirmed using a large spectrum of nonhuman DNAs. This report supports the use of the AmpFlSTR Profiler Plus STR system for casework DNA typing under reduced PCR volume conditions.
authors.affiliation: Royal Canadian Mounted Police, Forensic Laboratory Services, National DNA Data Bank of Canada, 1200 Vanier Parkway, Ottawa, Ontario.
authors.forename: Chantal J
authors.initials: CJ
authors.lastname: Frégeau
authors.forename: Kathy L
authors.initials: KL
authors.lastname: Bowen
authors.forename: Benoît
authors.initials: B
authors.lastname: Leclair
authors.forename: Isabelle
authors.initials: I
authors.lastname: Trudel
authors.forename: Lucy
authors.initials: L
authors.lastname: Bishop
authors.forename: Ron M
authors.initials: RM
authors.lastname: Fourney
mesh_terms: D000483:Alleles; D000818:Animals; D001419:Bacteria / Q000235:genetics; D016022:Case-Control Studies; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D004586:Electrophoresis; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences; D015003:Yeasts / Q000235:genetics
chemical_list: D004247:DNA