[Delayed biological consequences induced by gamma-radiation in human tumorigenic diploid line SK-UT-1B].
The progeny of SK-UT-1B cells that survived gamma-irradiation with 4 Gy up to the 80th passage was examined. Descendants of irradiated cells lost p53 transactivation properties. Simultaneously, in the presence of nocodazole coordination between M and S phases was disrupted. Meanwhile, descendants of irradiated cells maintained the accurate spindle assembly checkpoint. These data suggest that p53 transactivation function may be required for coordination of M and S phases, rather than for spindle assembly checkpoint. Since it is known that p53 regulates both these processes on the basis of data obtained, we suggest that functions of p53 required for coordination of M and S-phases and for spindle assembly checkpoint are separated. Besides, the data obtained indicate that radiation-induced chromosomal rearrangements are associated with activation of DNA recombination process.
Smirnova
I S
IS
Institute of Cytology RAS, St. Petersburg, Russia. smir@mail.cytspb.rssi.ru
Iakovleva
T K
TK
Aksenov
N D
ND
D000970:Antineoplastic Agents / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor / Q000528:radiation effects*; D002875:Chromosomes / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D015321:Gene Rearrangement; D006801:Humans; D008938:Mitosis; D015739:Nocodazole / Q000494:pharmacology; D011123:Polyploidy; D016196:S Phase; D008941:Spindle Apparatus / Q000235:genetics; D013997:Time Factors; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics
D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D015739:Nocodazole