title: Identification, characterization and subcellular localization of TcPDE1, a novel cAMP-specific phosphodiesterase from Trypanosoma cruzi.
abstract: Compartmentalization of cAMP phosphodiesterases plays a key role in the regulation of cAMP signalling in mammals. In the present paper, we report the characterization and subcellular localization of TcPDE1, the first cAMP-specific phosphodiesterase to be identified from Trypanosoma cruzi. TcPDE1 is part of a small gene family and encodes a 929-amino-acid protein that can complement a heat-shock-sensitive yeast mutant deficient in phospho-diesterase genes. Recombinant TcPDE1 strongly associates with membranes and cannot be released with NaCl or sodium cholate, suggesting that it is an integral membrane protein. This enzyme is specific for cAMP and its activity is not affected by cGMP, Ca2+, calmodulin or fenotiazinic inhibitors. TcPDE1 is sensitive to the phosphodiesterase inhibitor dipyridamole but is resistant to 3-isobutyl-1-methylxanthine, theophylline, rolipram and zaprinast. Papaverine, erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride, and vinpocetine are poor inhibitors of this enzyme. Confocal laser scanning of T. cruzi epimastigotes showed that TcPDE1 is associated with the plasma membrane and concentrated in the flagellum of the parasite. The association of TcPDE1 with this organelle was confirmed by subcellular fractionation and cell-disruption treatments. The localization of this enzyme is a unique feature that distinguishes it from all the trypanosomatid phosphodiesterases described so far and indicates that compartmentalization of cAMP phosphodiesterases could also be important in these parasites.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Maximiliano A
authors.initials: MA
authors.lastname: D'Angelo
authors.forename: Santiago
authors.initials: S
authors.lastname: Sanguineti
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Reece
authors.forename: Lutz
authors.initials: L
authors.lastname: Birnbaumer
authors.forename: Héctor N
authors.initials: HN
authors.lastname: Torres
authors.forename: Mirtha M
authors.initials: MM
authors.lastname: Flawiá
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D005407:Flagella / Q000201:enzymology; D040461:Gene Components; D005816:Genetic Complementation Test; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D013347:Subcellular Fractions / Q000737:chemistry; D014349:Trypanosoma cruzi / Q000201:enzymology*; D015003:Yeasts / Q000201:enzymology / Q000235:genetics
chemical_list: D015105:3',5'-Cyclic-AMP Phosphodiesterases