31P NMR studies on the putative chemoselective activation of nucleoside-3'-thiophosphate-O-esters.
P-diastereomerically pure O-esters of N(Bz)-5'-DMT-dA-3'-monothiophosphate, having charged S=P-O(-) moiety, have been synthesized. Chemoselectivity of their activations by formation of different mixed anhydrides, followed by couplings with N(Bz)-3'-levulinyl-dA, were studied by 31P NMR spectroscopy.
Sági
Gyula
G
Chemical Research Center, Institute of Chemistry, Hungarian Academy of Sciences, Budapest, Hungary. gsagi@chemres.hu
Bajor
Zoltán
Z
Mizda
Roland
R
Kenesi
Gyöngyi
G
D004952:Esters; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D009711:Nucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D010758:Phosphorus; D013237:Stereoisomerism
D004952:Esters; D007202:Indicators and Reagents; D009711:Nucleotides; D010710:Phosphates; D010758:Phosphorus; C035638:thiophosphoric acid