title: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow.
abstract: Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin alpha4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation.
authors.affiliation: Department of Surgery, UCLA School of Medicine, Regenerative Bioengineering and Repair Laboratory, Los Angeles, CA, USA. deugarte@ucla.edu
authors.forename: Daniel A
authors.initials: DA
authors.lastname: De Ugarte
authors.forename: Zeni
authors.initials: Z
authors.lastname: Alfonso
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Zuk
authors.forename: Amir
authors.initials: A
authors.lastname: Elbarbary
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Peter
authors.initials: P
authors.lastname: Ashjian
authors.forename: Prosper
authors.initials: P
authors.lastname: Benhaim
authors.forename: Mare H
authors.initials: MH
authors.lastname: Hedrick
authors.forename: John K
authors.initials: JK
authors.lastname: Fraser
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans
chemical_list: D000954:Antigens, Surface