Interaction between yeast eukaryotic initiation factor eIF4E and mRNA 5' cap analogues differs from that for murine eIF4E.
Measurements of interaction of 7-methyl-GTP eIF4E from S. cerevisiae were performed by means of two methods: Isothermal Titration Calorimetry (ITC) and fluorescence titration. The equilibrium association constants (Kas) derived from the two methods show significantly different affinity of yeast eIF4E for the mRNA 5' cap than those of the murine and human proteins. The observed differences in the Kas values and the enthalpy changes of the association (deltaH(o)) suggest some dissimilarity in the mode of binding and stabilization of cap in the complexes with eIF4E from various sources.
Kiraga-Motoszko
Katarzyna
K
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland.
Stepinski
Janusz
J
Niedzwiecka
Anna
A
Jemielity
Jacek
J
Wszelaka-Rylik
Malgorzata
M
Stolarski
Ryszard
R
Zielenkiewicz
Wojciech
W
Darzynkiewicz
Edward
E
D000818:Animals; D001665:Binding Sites; D002151:Calorimetry; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D012314:RNA Cap Analogs / Q000737:chemistry*; D012315:RNA Caps / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D012315:RNA Caps; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C017516:7-methylguanosine triphosphate