Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract.
Quantification of ellagic acid, the principal bioactive component of pomegranate leaf extract, in rats plasma following oral administration of pomegranate leaf extract was achieved by using a high-performance liquid chromatographic method. The calibration curve for ellagic acid was linear (r2=0.9998) ver the concentration range 0.026-1.3 microg/ml. The intra- and inter-day assays of ellagic acid from rat plasma were less than 6.52% at concentration range from 26 to 1300 ng/ml and good overall recoveries (94.5-102.4%) were found on same concentrations. The concentration-time profile was fitted with an open two-compartment system with lag time and its max concentration of ellagic acid in plasma was 213 ng/ml only 0.55 h after oral administration extract 0.8 g/kg. The pharmacokinetic profile indicates that ellagic acid has poor absorption and rapid elimination after oral administration pomegranate leaf extract, and part of it was absorbed from stomach.
Lei
Fan
F
Institute of Medicinal Plant, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100094, China.
Xing
Dong-Ming
DM
Xiang
Lan
L
Zhao
Yu-Nan
YN
Wang
Wei
W
Zhang
Lu-Jun
LJ
Du
Li-Jun
LJ
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000097:blood / Q000493:pharmacokinetics*; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D004610:Ellagic Acid