[Studies on antiendotoxin action of total saponins from radix Bupleuri].
OBJECTIVE
To study antiendotoxin activities of total saponins from Radix Bupleuri.


METHODS
The antiendotoxin activities were demonstrated by Limulus Amebocyte Lysate test (LAL test) in vitro, the fever model induced by endotoxin in rabbits and the model induced by LPS in BCG-primed mice.


RESULTS
Total saponins from Radix Bupleuri still had antiendotoxin effect even when it (50 mg.ml-1) was diluted to 32 times by LAL test in vitro. The fever experiment results showed the average rising temperature of BCS + LPS, group, model group and BCS group was 0.75 degrees C, -0.25 degrees C and -0.37 degrees C respectively. In the experiment of the poison induced by LPS, fatal rate of model group was 90.0%, that of BCS + LPS group was 13.3%, there was a significant difference (P < 0.01).


CONCLUSIONS
The result showed that the total saponins in Bupleuri had the strongest antiendotoxin action.
Liu
Yunhai
Y
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Chen
Yongshun
Y
Xi
Wei
W
Bai
Jie
J
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D029972:Bupleurum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D005260:Female; D005334:Fever / Q000139:chemically induced / Q000503:physiopathology; D008033:Limulus Test; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008070:Lipopolysaccharides; D012503:Saponins