title: [Protective effects of tanshinone IIA on injured primary cultured rat hepatocytes induced by CCl4].
abstract: OBJECTIVE
  To investigate the effect of tanshinone IIA on carbon tetrachloridlde (CCl4) intoxicated primary cultured rat hepatocytes.
  
  
  METHODS
  The rat hepatocytes were separated by perfusion with collagenase solution. Tanshinone IIA was added to the cultured medium after incubating the rat hepatocytes for 36 hours. At the same time, CCl4 was added to induce hepatocytes injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined respectively.
  
  
  RESULTS
  Tanshinone IIA significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels
  
  
  CONCLUSIONS
  Tanshinone IIA protected effectively the primary cultured rat hepatocytes against CCl4-induced injury.
authors.affiliation: Center Hospital of Xi'an, Xi'an 710003.
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045784:Abietanes; D000818:Animals; D002251:Carbon Tetrachloride; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000378:metabolism; D008297:Male; D010616:Phenanthrenes / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry
chemical_list: D045784:Abietanes; D010616:Phenanthrenes; D020011:Protective Agents; C021751:tanshinone; D002251:Carbon Tetrachloride