title: Isolation of lectins by affinity chromatography with porcine plasma proteins immobilized on agarose.
abstract: To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues.
authors.affiliation: School of Food and Nutritional Sciences, University of Shizuoka, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Kajiya
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Mita
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mega
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Hase
authors.forename: Toshioki
authors.initials: T
authors.lastname: Kawakami
authors.forename: Eiko
authors.initials: E
authors.lastname: Honda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Munakata
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000818:Animals; D027983:Aralia / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry*; D013552:Swine; D031228:Wasabia / Q000737:chemistry
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D037121:Plant Lectins; D012685:Sepharose