[The effect of specific virus delivering of Langerhans cell for the M-tropic HIV infection].
OBJECTIVE
To observe the cellular mechanism of via skin infection of Microphage tropic (M-tropic) HIV and further differentiate the infection of M-tropic and T-tropic HIV.


METHODS
DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope. Emigrating cells were Mac-sorted into CD80+ and CD80-. The infection of CD4+ T lymphocytes by HIV from emigrating cells were further assessed by PCR and RT assay.


RESULTS
Through direct transfection of skin by Gene Gun, emigrating cells from skin showed transfection of AD8 and NL43 and the gag gene can be amplified from the cells. Only AD8 transfected emigrating cells can further infect CD4+ T lymphocyte. Gag can be amplified and reverse transcriptase can be expressed from these CD4+ T cells; NL43 transfected emigrating cells can not infect CD4+ T lymphocyte. No gag can be amplified and no reverse transcriptase can be expressed. AD8 transfected and 1/2 log diluted CD80+ cells can infect CD4+ T lymphocyte. Even after 4 dilutions, there are still a lot of CD4+ T cells, gag can be amplified and a large amount of reverse transcriptase was expressed that is double or triple of controls. AD8 infected CD80-cells co-cultured with CD4+ T cells showed gag amplification only in first dilution and reverse transcriptase is also expressed. Gag can not be amplified by further dilution. NL43 infected CD80+ or CD80- emigrating cells co-cultured with CD4+ T cells did not show gag amplification and did not express reverse transcriptase.


CONCLUSIONS
Direct AD8 transfected CD80+ emigrating epidermal cells can specifically infect CD4+ T lymphocyte. This specificity not only depends on affinitive combination of HIV receptor on cell surface, but also on the specific virus delivering of AD8 transfected CD80+ cells that infect the CD4+ T cells. That means even without the receptor, CD80+ cells only deliver transfected AD8 but not NL43. So, it can be said that the specificity of the virus receptor is not the only clue of HIV infection.
Shi
Wei-min
WM
Lab Center, Tongji Hospital, Tongji University, Shanghai 200065, China.
Cameron
Paul U
PU
Purcell
Damian J F
DJ
D000256:Adenoviridae / Q000235:genetics; D019470:Biolistics; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D006678:HIV / Q000502:physiology*; D006801:Humans; D007801:Langerhans Cells / Q000821:virology*; D008264:Macrophages / Q000821:virology; D014162:Transfection / Q000379:methods*