title: Changes in microcirculation after ischemic process in rat skeletal muscle.
abstract: Laser Doppler flowmetry (LDF) is a good method to investigate tissue microcirculation, but it has many standardization and measuring problems. To exclude these effects, we performed a test using LDF on rat skeletal muscle. In 12 CD outbred anesthetized rats, bilateral femoral vessels and the quadriceps femoris muscle were exposed. The left femoral artery and vein were clamped for 1 h by microvascular clips (ischemic side). On the right side, no other intervention was made (control side). An LDF probe was applied on the medial vastus muscle. Short-term occlusions (2-3 s) were performed before and after the 1-h clamping and on the control side while LDF curves were registered. The halftimes of ascending curves on the ischemic side were significantly elongated vs. the condition before clamping (P = 0.0007) or the control side (P = 0.0017). Microcirculatory changes caused by 1-h ischemia were shown by this simple, quick, and well-reproducible test on rat skeletal muscle.
authors.affiliation: Department of Operative Techniques and Surgical Research, Medical and Health Science Center, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. nemeth@jaguar.dote.hu
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.forename: Tamas
authors.initials: T
authors.lastname: Lesznyak
authors.forename: Endre
authors.initials: E
authors.lastname: Brath
authors.forename: Gabor
authors.initials: G
authors.lastname: Acs
authors.forename: Arpad
authors.initials: A
authors.lastname: Nagy
authors.forename: Jozsef
authors.initials: J
authors.lastname: Pap-Szekeres
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
mesh_terms: D000818:Animals; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D008833:Microcirculation / Q000000981:diagnostic imaging / Q000503:physiopathology*; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000503:physiopathology; D051381:Rats; D015427:Reperfusion Injury / Q000000981:diagnostic imaging / Q000503:physiopathology*; D014463:Ultrasonography