title: [Humoral immune response in breeding sows after vaccination with a herd-specific Chlamydophila abortus vaccine].
abstract: A chlamydial vaccine efficacy trial with assessment of the clinical acceptability and serum antibody responses was performed in breeding sows. A BGM cell culture derived vaccine containing 10(8)/ml formalin-inactivated purified elementary bodies (Eb.) in sterile 0.15 M saline was prepared from Chlamydophila (Ch.) abortus strain OCHL03/99 which has been isolated in the herd from a sample of vaginal discharge. Vaccination was performed as a randomised trial with parallel treatment of a vaccinated group (25 sows) and non-vaccinated control group (20 sows). Sows received two 2.0-ml doses of vaccine intramuscularly at a three week interval. Control sows were dosed with sterile 0.15 M saline, accordingly. Serological response to vaccination was measured by ELISA with a total of 204 blood serum samples (114 from the vaccine group; 90 from the control group) using crude chlamydial LPS as the antigen. Compared to the control group, vaccinated sows showed a marked primary and secondary IgG serum antibody response following the two vaccinations. Antibody levels peaked between week 7 and 14 after priming vaccination, declined incrementally until week 27 but remained significantly higher than the corresponding sham-immune control levels and the prevaccination values of the vaccine group (p < 0.05). Western blot analysis of solubilized whole Eb. of Ch. abortus, Ch. pecorum, and Chlamydia (C.) suis with pre- and postvaccination sera confirmed that vaccination induced an antibody response preferentially against a range of 13 chlamydial antigens including the 40 kDa MOMP of Ch. abortus. Clinical side effects consisting of a transient mild local inflammatory reaction at the site of injection were observed in approx. 30% of vaccinated sows. These results provide the basis for further clinical evaluation of the Ch. abortus vaccine to protect sows from chlamydia-induced reproductive disorders.
authors.affiliation: Institut für Veterinärbakteriologie, Universität Zürich, Schweiz.
authors.forename: J C
authors.initials: JC
authors.lastname: Knitz
authors.forename: L E
authors.initials: LE
authors.lastname: Hoelzle
authors.forename: P
authors.initials: P
authors.lastname: Affolter
authors.forename: A
authors.initials: A
authors.lastname: Hamburger
authors.forename: K
authors.initials: K
authors.lastname: Zimmermann
authors.forename: K
authors.initials: K
authors.lastname: Heinritzi
authors.forename: M M
authors.initials: MM
authors.lastname: Wittenbrink
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D016896:Treatment Outcome; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines