title: Facile and selective nanoscale labeling of peptides in solution by using photolabile protecting groups.
abstract: For the selective labeling of peptides, a novel strategy was developed that combines the advantages of solid-phase peptide synthesis with the flexibility of labeling reactions in solution. To direct a label at a distinct position within the peptide sequence, other reactive positions are blocked with photolabile protecting groups that could be easily removed after the labeling reaction. Therefore selective labeling may become feasible for the first time even in nanomol amounts.
authors.affiliation: Institute of Biochemistry, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Koglin
authors.forename: Manja
authors.initials: M
authors.lastname: Lang
authors.forename: Robert
authors.initials: R
authors.lastname: Rennert
authors.forename: Annette G
authors.initials: AG
authors.lastname: Beck-Sickinger
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000138:chemical synthesis; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D036103:Nanotechnology; D009478:Neuropeptide Y / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D017476:Receptors, Neuropeptide Y / Q000187:drug effects / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D005449:Fluorenes; D007202:Indicators and Reagents; D009478:Neuropeptide Y; D010455:Peptides; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor; C054007:1-(9-fluorenyl)methyl chloroformate; D015740:Calcitonin Gene-Related Peptide