title: [Polymorphism in codons 10 and 25 of the transforming growth factor-beta1 gene in Korean population and in patients with liver cirrhosis and hepatocellular carcinoma].
abstract: OBJECTIVE
  The genetic polymorphism of transforming growth factor-beta1 (TGF-beta1) at codons 10 and 25 which influences the production of TGF-beta1 is related to fibrogenesis in the lung and liver. We evaluated the genetic polymorphism at codons 10 and 25 in controls and in patients with liver cirrhosis (LC) and hepatocellular carcinoma (HCC).
  
  
  METHODS
  Blood samples were collected from controls (n=35), patients with LC (n=64), and HCC (n=49). Genomic DNA was isolated and polymerase chain reaction (PCR) was done for a segment including codons 10 and 25. The results of direct sequencing for PCR products were compared between the controls and the patients.
  
  
  RESULTS
  There was no genetic polymorphism at codon 25 and three types of genetic polymorphism at codon 10. The leucine homozygous genotype (CTG/CTG) at codon 10 was more common in patients with LC than the controls (p=0.01) and especially in patients with LC caused by HBV (p=0.004). The polymorphism at codons 10 in patients with HCC was similar to the controls. However, leucine homozygous genotype was more common in patients with HCC of uninodular morphology than those of massive morphology (p=0.007).
  
  
  CONCLUSIONS
  The genetic polymorphism of TGF-beta1 at codon 10 might be associated with LC and morphology of HCC. The potential usefulness of TGF-beta1 genotyping needs further studies in large scale.
authors.affiliation: Gachon Medical School, Inchon, Korea.
authors.forename: Oh Sang
authors.initials: OS
authors.lastname: Kwon
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Song
authors.forename: Ki Tak
authors.initials: KT
authors.lastname: Ju
authors.forename: Moon Gi
authors.initials: MG
authors.lastname: Chung
authors.forename: Dong Kyun
authors.initials: DK
authors.lastname: Park
authors.forename: Sun Suk
authors.initials: SS
authors.lastname: Kim
authors.forename: Yeon Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Yang Suh
authors.initials: YS
authors.lastname: Koo
authors.forename: Yu Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Duck Joo
authors.initials: DJ
authors.lastname: Choi
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: You Jin
authors.initials: YJ
authors.lastname: Hwang
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Byun
authors.forename: Chang Hong
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D003062:Codon / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007723:Korea; D008103:Liver Cirrhosis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020539:Sequence Analysis, Protein; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1
chemical_list: D003062:Codon; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1