title: Occluding effect of dentifrices on dentinal tubules.
abstract: OBJECTIVE
  The objective of this study was to evaluate the tubule occluding ability of three commercial available dentifrices (Sensodyne, Emoform and Sorriso) by Scanning Electron Microscopy.
  
  
  METHODS
  Fifty cervical areas from buccal and lingual surfaces of sound third human molars were used. Cervical enamel was wet abraded to expose flat dentin surfaces and further polished with diamond pastes. Specimens were randomly divided into five groups (n=10): G1-no brushing; G2-brushing without dentifrice; G3-brushing with Sensodyne; G4-brushing with Emoform; G5-brushing with Sorriso. Brushed specimens were treated for 4 min per day, for 7 days in a toothbrushing machine. Specimens were prepared and observed under SEM for calculation of the percentage of occluded tubules. In addition, slurries of toothpastes were analyzed by X-ray microanalysis. Data were statistically analyzed by ANOVA and Tukey test (p<0.05).
  
  
  RESULTS
  Means of occluded tubules in descending order were: G5-98.83+/-3.31% (a), G4-96.02+/-5.24% (a), G3-80.12+/-24.65% (a), G1-37.31+/-24.22% (b); G2-22.92+/-15.19% (b). The three tested dentifrices produced increased dentinal occlusion as compared to controls (p<0.05) but equivalent occlusion among each other. Calcium from calcium-carbonate abrasive was identified in all dentifrices.
  
  
  CONCLUSIONS
  Results indicated that the use of all dentifrices occluded tubules more than no brushing and brushing without dentifrices groups. Thus, the tested dentifrices seem effective for desensitization by tubule occlusion.
authors.affiliation: Department of Restorative Dentistry/Operative Dentistry, Piracicaba School of Dentistry, UNICAMP. Av. Limeira, #901, Piracicaba, SP 13414-900, Brazil.
authors.forename: César Augusto Galvão
authors.initials: CA
authors.lastname: Arrais
authors.forename: Carolina Diniz
authors.initials: CD
authors.lastname: Micheloni
authors.forename: Marcelo
authors.initials: M
authors.lastname: Giannini
authors.forename: Daniel C N
authors.initials: DC
authors.lastname: Chan
mesh_terms: D000704:Analysis of Variance; D002119:Calcium Carbonate / Q000032:analysis; D003802:Dentifrices / Q000032:analysis / Q000494:pharmacology*; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004338:Drug Combinations; D004577:Electron Probe Microanalysis; D005459:Fluorides / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009566:Nitrates / Q000032:analysis / Q000494:pharmacology; D010710:Phosphates / Q000032:analysis / Q000494:pharmacology; D019237:Tooth Cervix / Q000187:drug effects / Q000648:ultrastructure; D014099:Toothbrushing / Q000295:instrumentation
chemical_list: D003802:Dentifrices; D004338:Drug Combinations; D009566:Nitrates; D010710:Phosphates; C097427:Sensodyne; D002119:Calcium Carbonate; D005459:Fluorides