[Beta-1 and beta-4 integrins, and laminin 67 kDa receptors in interaction between A431 cells and laminin isoforms ].
Laminins, as basal membrane glycoproteins, are able to stimulate cell adhesion and migration, and to influence gene expression. The laminin molecule has a set of bioactive sites that interact with different integrin and nonintegrin receptors, and, as a result, the reaction of the same cell type to different laminin isoforms may be different. The aim of this study was to determine the contributions of both integrins with beta 1 and beta 4 chains and 67 kDa laminin receptor in the interaction of A431 cells with two laminin isoforms: laminin-1 and laminin-2/4. The obtained data show that integrin alpha 6 beta 4 is more specific for interaction with laminin-2/4 than with laminin-1 and takes part in the stage of attachment of A431 cells to laminin. 67 kDa receptor promotes cell spreading on laminin-2/4 and inhibits cell spreading on laminin-1. An assumption was made about the complex action of receptors for interaction of A431 cells with laminins ("integrin alpha 6 beta 4--67 kDa receptors" complex).
Cherepanova
O A
OA
Institute of Cytology RAS, St. Petersburg. cheer@mailru-com
Kalmykova
N V
NV
Blinova
M I
MI
Pinaev
G P
GP
D001485:Basement Membrane / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism* / Q000502:physiology; D039663:Integrin beta4 / Q000378:metabolism* / Q000502:physiology; D007797:Laminin / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D017474:Receptors, Laminin / Q000378:metabolism* / Q000502:physiology
D019012:Integrin beta1; D039663:Integrin beta4; D007797:Laminin; D020033:Protein Isoforms; D017474:Receptors, Laminin