Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.
[reaction: see text] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.
Adamczyk
Maciej
M
Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbot.com
Mattingly
Phillip G
PG
Moore
Jeffrey A
JA
Pan
You
Y