The human papillomavirus HPV2a E5 protein localizes to the Golgi apparatus and modulates signal transduction.
The low-risk human papillomavirus type 2a (HPV2a) has been found associated with benign skin epithelial tumors and has only been very rarely identified in malignized epithelia. Here we report the identification of the E5 gene of HPV2a and demonstrate that the protein is mainly expressed in the Golgi apparatus of transfected cells, similar to the known high-risk types E5 proteins. Further, we present experimental evidence demonstrating that HPV2a E5, similar to HPV16 E5, is able to modulate EGF-mediated erk1/2 MAP kinase activation and to down-regulate the expression of MHC class I molecules at the plasma membrane. Thus, the E5 gene of at least one cutaneous low-risk HPV type displays similar biological characteristics to those described for the high-risk type HPV16.
Cartin
Walter
W
Deutsches Krebsforschungszentrum, Im Neuenheimer Feld-242, 69120 Heidelberg, Germany.
Alonso
Angel
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D015536:Down-Regulation; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
D015395:Histocompatibility Antigens Class I; D009856:Oncogene Proteins, Viral; D004815:Epidermal Growth Factor; D019950:Mitogen-Activated Protein Kinase 1