Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with multisubstrate analogue inhibitor with 2,6-diaminopurine aglycone.
The crystal structure at 2.05 A resolution of calf spleen PNP complexed with stoichiometric concentration of acyclic nucleoside phosphonate inhibitor, 2,6-diamino-(S)-9-[2-(phosphonomethoxy)propyl]purine, in a new space group P2(1)2(1)2(1) which contains two full trimers in the asymmetric crystal unit is described.
Koellner
Gertraud
G
Institut für Chemie-Kristallographie, Freie Universität Berlin, Berlin, Germany.
Stroh
Albrecht
A
Raszewski
Grzegorz
G
Holý
Antonin
A
Bzowska
Agnieszka
A
D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray / Q000379:methods; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology*; D013154:Spleen / Q000201:enzymology
C479442:2,6-diaminopurine aglycone; D004791:Enzyme Inhibitors; D046911:Macromolecular Substances; D011687:Purines; D004317:Doxorubicin; D011683:Purine-Nucleoside Phosphorylase