Correlation of death modes of photosensitized cells with intracellular ATP concentration.
The impact of intensity of glycolysis and oxidative phosphorylation on death of photosensitized murine hepatoma MH22 cells in vitro has been investigated. Cells photosensitized with meso-tetra(4-sulfonatophenyl)-porphine localized to lysosomes died mostly by necrosis, and the mode of cell death did not depend on the energy metabolism. Photosensitization with 5-aminolevulinic acid-stimulated endogenous porphyrins localized mainly in mitochondria or 5,10,15,20-tetrakis(m-hydroxyphenyl)-chlorine localized to cell membranes, including mitochondria, led to cell death mostly by apoptosis. In this case, the mode of cell death depended on the medium: under conditions unfavorable to glycolysis the ratio apoptosis/necrosis decreased significantly.
Kirveliene
Vida
V
Department of Biochemistry and Biophysics, Vilnius University, Lithuania. vida.kirveliene@gf.vu.lt
Sadauskaite
Ausra
A
Kadziauskas
Jurgis
J
Sasnauskiene
Sofija
S
Juodka
Benediktas
B
D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000494:pharmacology*; D000622:Aminolevulinic Acid / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D006019:Glycolysis / Q000187:drug effects / Q000528:radiation effects; D007424:Intracellular Fluid / Q000378:metabolism*; D008027:Light; D008247:Lysosomes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009336:Necrosis; D010085:Oxidative Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D011166:Porphyrins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011166:Porphyrins; C016136:tetraphenylporphine sulfonate; D000622:Aminolevulinic Acid; D000255:Adenosine Triphosphate