Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms.
Three 26 kDa proteins, named as TJ-CRVP, NA-CRVP1 and NA-CRVP2, were isolated from the venoms of Trimeresurus jerdonii and Naja atra, respectively. The N-terminal sequences of TJ-CRVP and NA-CRVPs were determined. These components were devoid of the enzymatic activities tested, such as phospholipase A(2), arginine esterase, proteolysis, L-amino acid oxidase, 5'nucleotidase, acetylcholinesterase. Furthermore, these three components did not have the following biological activities: coagulant and anticoagulant activities, lethal activity, myotoxicity, hemorrhagic activity, platelet aggregation and platelet aggregation-inhibiting activities. These proteins are named as cysteine-rich venom protein (CRVP) because their sequences showed high level of similarity with mammalian cysteine-rich secretory protein (CRISP) family. Recently, some CRISP-like proteins were also isolated from several different snake venoms, including Agkistrodon blomhoffi, Trimeresurus flavoviridis, Lanticauda semifascita and king cobra. We presumed that CRVP might be a common component in snake venoms. Of particular interest, phylogenetic analysis and sequence alignment showed that NA-CRVP1 and ophanin, both from elapid snakes, share higher similarity with CRVPs from Viperidae snakes.
Jin
Yang
Y
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, People's Republic of China. jinyangkiz@hotmail.com
Lu
Qiumin
Q
Zhou
Xingding
X
Zhu
Shaowen
S
Li
Rui
R
Wang
Wanyu
W
Xiong
Yuliang
Y
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D003545:Cysteine / Q000737:chemistry; D004487:Edema / Q000139:chemically induced; D004546:Elapid Venoms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000633:toxicity; D017815:Elapidae; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D007082:Ileum / Q000502:physiology; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D017841:Trimeresurus
D004546:Elapid Venoms; D011506:Proteins; D003545:Cysteine