The HPLC determination of propane-1,2,3-triyl trinitrite and impurities: (2RS)-3-hydroxypropane-1,2-diyl dinitrate and 2-hydroxypropane-1,3-diyl dinitrate in ointment.
A HPLC method for determination of propane-1,2,3-triyl trinitrate and impurities: (2RS)-3-hydroxypropane-1,2-diyl dinitrate and 2-hydroxypropane-1,3-diyl dinitrate ointment was developed. The conditions for good separation of constituents, while avoiding vehiculum interference were established. The results feature of high accuracy and good precision. For individual constituents R.S.D. is ranged from 0.7 to 9.9%, while recovery was 100.1% for propane-1,2,3-triyl trinitrate and 95.1-99.0% for impurities. It has been found that propane-1,2,3-triyl trinitrate used in medicine in the form of ointment contains such impurities which can be identified and quantified at relatively low concentrations of 70 ng ml(-1).
Krzek
Jan
J
Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University Cracow, 9 Medyczna Street, 30 688 Cracow, Poland.
Moniczewska
Maria
M
Zabierowska-Slusarczyk
Grazyna
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005996:Nitroglycerin / Q000032:analysis* / Q000737:chemistry; D009824:Ointments; D011407:Propane / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry
D009824:Ointments; D005996:Nitroglycerin; D011407:Propane