title: Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G).
abstract: We recently identified a novel human sialic acid binding immunoglobulin-like lectin, Siglec-8, using mRNA from human eosinophils. To search for a mouse Siglec (mSiglec) ortholog of Siglec-8 and other mouse Siglec paralogs, we conducted public database searches with cDNA sequences of human Siglec-5 to -10 and identified two novel mSiglecs. One has significant sequence identity to human Siglec-5 and is a splice variant of mSiglec-F. The other has greatest sequence identity to human Siglec-10 (mSiglec-G). Both mSiglecs have extracellular Ig-like domains and intracellular tyrosine-based motifs. To determine whether these mSiglecs were relevant to mouse eosinophils, RT-PCR and Northern blot analysis were performed. We detected expression of mSiglec-5 (or -F), -10, and -E mRNA in purified mouse eosinophils, but Northern blot data comparing expression in tissues from normal, IL-5 transgenic, and allergen-sensitized and -challenged mice suggest that mSiglec-10 is probably most relevant to mouse eosinophils.
authors.affiliation: Department of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Aizawa
authors.forename: Nives
authors.initials: N
authors.lastname: Zimmermann
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Carrigan
authors.forename: James J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Marc E
authors.initials: ME
authors.lastname: Rothenberg
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Bochner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry / Q000235:genetics*; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry* / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D030561:Databases, Nucleic Acid; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D018076:DNA, Complementary; D037102:Lectins; C403967:SIGLEC8 protein, human; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C443130:Siglecf protein, mouse