[Detection and analysis of SARS coronavirus-specific antibodies in sera from non-SARS children].
OBJECTIVE
To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children.


METHODS
Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1,060 non-SARS children in Guangzhou.


RESULTS
All the serum samples from the 1,060 non-SARS children were negative for both IgG and IgM antibodies against SARS coronavirus as determined by indirect immunofluorescent assay, with only two serum samples showing weak positivity for SARS coronavirus-specific antibodies identified by double-antigen sandwich ELISA.


CONCLUSIONS
No SARS coronavirus-specific antibody are present in the sera of non-SARS children.
Li
Lin-hai
LH
Department of Clinical Laboratory, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. linhaili@hotmail.com
Shi
Yu-ling
YL
Li
Ping
P
Xu
De-xing
DX
Wan
Gen-ping
GP
Gu
Xiao-qiong
XQ
Zhang
Xiao-ling
XL
Ma
Qing-jun
QJ
Cao
Cheng
C
D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*
D000914:Antibodies, Viral