Genetic characterization of wild-type measles viruses in Cambodia.
Cambodian authorities in collaboration with the World Health Organization (WHO) are implementing a measles control plan in this measles endemic country. Genetic characterization of Cambodian wild-type measles viruses was performed to determine the genotypes involved in outbreaks, and to measure the level of virus circulation in a geographic area just beginning to implement the measles control program. Seventy-two sequences of the C terminus of the nucleoprotein gene of measles virus were obtained from 88 patients. Samples were taken from 35 among 519 outbreaks reported to the Cambodian National Immunization Program between March 2001 and June 2002. The sequences were grouped into 10 lineages which all belonged to genotype D5. The maximum nucleotide divergence was 1.3%.
Horm
Srey Viseth
SV
Institut Pasteur du Cambodge, 5 Monivong Boulevard, BP 983, Phnom Penh, Cambodia.
Dumas
Céline
C
Svay
Sarath
S
Feldon
Keith
K
Reynes
Jean-Marc
JM
D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D002160:Cambodia / Q000453:epidemiology; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008457:Measles / Q000453:epidemiology / Q000821:virology*; D008459:Measles virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014556:Urine / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus