Comparative study of matrix-assisted laser desorption/ionization and gas chromatography for quantitative determination of cocoa butter and cocoa butter equivalent triacylglycerol composition.
The triacylglycerol (TAG) composition study of cocoa butter (CB) and cocoa butter equivalents (CBEs) has been performed by gas chromatography (GC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). These two techniques provided comparable results. The advantage of the MALDI technique was the detection of each compound comprising the triacylglycerol classes (Cn). Moreover, comparison of the data obtained by these two techniques indicated that TAG relative percentages could be obtained quantitatively with the MALDI technique. These techniques have been applied for the composition determination of CB + CBE mixtures. Encouraging results showed that it is possible to quantify an admixture containing as little as 4% of CBE.
Guyon
F
F
Laboratoire DGCCRF Bordeaux, 351 cours de la Libération, F33405 Talence Cedex, France. labo33@dgccrf.finances.gouv.fr
Absalon
Ch
Ch
Eloy
A
A
Salagoity
M H
MH
Esclapez
M
M
Medina
B
B
D000349:Africa; D002099:Cacao / Q000737:chemistry; D002849:Chromatography, Gas; D004041:Dietary Fats / Q000032:analysis*; D012996:Solutions; D013020:South America; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry
D004041:Dietary Fats; D012996:Solutions; D014280:Triglycerides; C052387:cocoa butter