Green tea epigallocatechin-3-gallate is an inhibitor of mammalian histidine decarboxylase.
(-)-epigallocatechin-3-gallate, an antiproliferative and antiangiogenic component of green tea, has been reported to inhibit dopa decarboxylase. In this report,we show that this compound also inhibits histidine decarboxylase, the enzymic activity responsible for histamine biosynthesis. This inhibition was proved by a double approach, activity measurements and UV-Vis spectra of enzyme-bound pyridoxal-5'-phosphate. At 0.1 mM (-)-epi-gallocatechin-3-gallate, histidine decarboxylase activity was inhibited by more than 60% and the typical spectrum of the internal aldimine form shifted to a stable major maximum at 345 nm, suggesting that the compound causes a stable change in the structure of the holoenzyme. Since histamine release is one of the primary events in many inflammatory responses, a new potential application of (-)-epigallocatechin-3-gallate in prevention or treatment of inflammatory processes is suggested by these data.
Rodríguez-Caso
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C
Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, Málaga, Spain.
Rodríguez-Agudo
D
D
Sánchez-Jiménez
F
F
Medina
M A
MA
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006632:Histamine / Q000096:biosynthesis; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D051381:Rats; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D013662:Tea / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D013662:Tea; D006632:Histamine; D002392:Catechin; C045651:epigallocatechin gallate; D006640:Histidine Decarboxylase