[FHIT gene is abnormal in tongue carcinoma cell line].
To study the alteration of FHIT gene in tongue carcinoma Tca8113 cell line, total RNA of Tca8113 cells was extracted. The transcript of the FHIT gene of the Tca8113 cell line was detected with nest RT-PCR, and DNA was sequenced. The result showed that abnormal transcript (about 247 bp) of FHIT gene was detected in the Tca8113 cell line. The sequence analysis of the aberrant cDNAs revealed deletions of exons 1-8. Therefore, the deletion of the FHIT gene in Tca8113 cell line might support the hypothesis that the FHIT gene alteration is involved in the development of tongue carcinoma.
Liu
Feng
F
Department of Oral Biology, Stomatology College, Fourth Military Medical University, Xi'an 710032.
Wu
Junzheng
J
Sun
Yefang
Y
D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D001483:Base Sequence; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014062:Tongue Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase