Functional disturbance of biliary indocyanine green excretion in rat cerulein pancreatitis followed by endotoxemia: role of the prime and the second attack.
BACKGROUND
Hepatic injury is considered one of the critical complications associated with acute pancreatitis. It was proposed that initial insults to the liver in the early phase of the attack have an important priming effect, and the subsequent infectious attack (e.g. infectious pancreatic necrosis, bacterial translocation episode) constitutes a second attack on the liver.


OBJECTIVE
To evaluate the role of priming by induction of cerulein pancreatitis and a following second attack by endotoxemia.


METHODS
Plasma clearance and biliary excretion of indocyanine green (a hepatophillic hydrophobic organic anion).


METHODS
A model of acute pancreatitis in rats.


METHODS
Four groups of rats: untreated control, cerulein pancreatitis, endotoxemia and endotoxemia following the induction of cerulein pancreatitis (pancreatitis + endotoxemia).


RESULTS
Biliary indocyanine green excretion was significantly disturbed only in the pancreatitis + endotoxemia group. Plasma clearance (a reflection of hepatic uptake) of indocyanine green from the blood was only slightly affected in endotoxemia group.


CONCLUSIONS
Biliary secretion is quite sensitive to this hepatic injury model. Both the preceding priming insult and the following second attack are important in the development of hepatic injury.
Okabe
Akihiro
A
Department of Surgery II, Kumamoto University Medical School. Kumamoto, Japan. mhirota@kaiju.medic.kumamoto-u.ac.jp
Hirota
Masahiko
M
Kimura
Yu
Y
Maeda
Keisuke
K
Ogawa
Michio
M
D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001646:Bile / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000503:physiopathology; D002108:Ceruletide / Q000633:toxicity*; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000097:blood / Q000139:chemically induced* / Q000201:enzymology / Q000503:physiopathology*; D007208:Indocyanine Green / Q000493:pharmacokinetics*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008111:Liver Function Tests / Q000379:methods; D008297:Male; D010195:Pancreatitis / Q000097:blood / Q000139:chemically induced* / Q000201:enzymology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D002108:Ceruletide; D000410:Alanine Transaminase; D007208:Indocyanine Green