Effects of iron limitation on adherence and cell surface carbohydrates of Corynebacterium diphtheriae strains.
Iron limitation may cause bacterial pathogens to grow more slowly; however, it may also stimulate these microorganisms to produce greater tissue damage, given that many virulence factors are controlled by the iron supply in the environment. The present study investigated the influence of low iron availability on the expression of proteins and surface sugar residues of two toxigenic strains of Corynebacterium diphtheriae subsp. mitis and evaluated their adherence to human group B erythrocytes and HEp-2 cells. A comparison was made between bacteria grown in (i) Trypticase soy broth (TSB), (ii) TSB treated with dipyridyl to deplete free iron, and (iii) TSB enriched with FeCl(3). The effects of iron concentration on adhesive properties were different for strains 241 and CDC-E8392, of the sucrose-fermenting and non-sucrose-fermenting biotypes, respectively. Iron-limited conditions enhanced interaction of strain 241 with erythrocytes and HEp-2 cells. Inhibition assays suggested the involvement of nonfimbrial protein combination 67-72p on hemagglutination of diphtheria bacilli grown under iron-limited conditions. Conversely, iron limitation inhibited adherence to glass and expression of electron-dense material on the bacterial surface. Lectin binding assays demonstrated a reduction in the number of sialic acid residues and an increase in D-mannose and D-galactose residues on the surfaces of both strains. Thus, iron exerts a regulatory role on adhesive properties of diphtheria bacilli, and low iron availability modulates the expression of C. diphtheriae surface carbohydrate moieties. The significant changes in the degree of lectin binding specific for D-mannose, D-galactose and sialic acid residues may have an effect on binding of host cells. The expression of dissimilar microbial virulence determinants may be coordinately controlled by common regulatory systems. For C. diphtheriae, the present results imply regulation of adherence and slime production as part of a global response to iron-limited environmental conditions that includes derepression of genes for the synthesis of cytotoxin and siderophores and for transport of the Fe(III)-siderophore complexes.
Moreira
Lílian de Oliveira
LO
Faculdade de Ciências Médicas. Instituto de Biologia Prof. Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Andrade
Arnaldo Feitosa Braga
AF
Vale
Márcio Damasceno
MD
Souza
Sônia Maria Silva
SM
Hirata
Raphael
R
Asad
Lídia Maria Oliveira Buarque
LM
Asad
Nasser Ribeiro
NR
Monteiro-Leal
Luiz Henrique
LH
Previato
José Osvaldo
JO
Mattos-Guaraldi
Ana Luiza
AL
D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003353:Corynebacterium diphtheriae / Q000472:pathogenicity* / Q000502:physiology; D004912:Erythrocytes / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005898:Glass; D006384:Hemagglutination; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D037102:Lectins / Q000378:metabolism; D008854:Microscopy, Electron
D001426:Bacterial Proteins; D037102:Lectins; D007501:Iron