Size and stability of reconstituted sesame oil bodies.
Oil bodies of sesame seeds comprise a triacylglycerol matrix, which is surrounded by a monolayer of phospholipids embedded with unique proteins, mainly structural proteins termed oleosins. Artificial oil bodies were successfully reconstituted with various compositions of triacylglycerols, phospholipids, and oil-body proteins. The sizes of reconstituted oil bodies displayed a normal distribution with an average size proportional to the ratio of triacylglycerols to oil-body proteins. Both thermostability and structural stability of reconstituted oil bodies decreased as their sizes increased, and vice versa. Proteinase K digestion indicated that oleosins anchored both native and reconstituted oil bodies via their central hydrophobic domains. The stability of reconstituted oil bodies, as well as the purified ones from sesame seeds, could be substantially enhanced after their surface proteins were cross-linked by glutaraldehyde or genipin.
Peng
Chi-Chung
CC
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
Lin
I-Ping
IP
Lin
Ching-Kuan
CK
Tzen
Jason T C
JT
D006863:Hydrogen-Ion Concentration; D002479:Inclusion Bodies / Q000378:metabolism* / Q000648:ultrastructure*; D012639:Seeds / Q000166:cytology* / Q000378:metabolism*; D012715:Sesame Oil / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D031685:Sesamum / Q000166:cytology* / Q000378:metabolism*; D013499:Surface Properties; D013696:Temperature
D012715:Sesame Oil