title: Occurrence of the allene oxide cyclase in different organs and tissues of Arabidopsis thaliana.
abstract: Occurrence of an essential enzyme in jasmonate (JA) biosynthesis, the allene oxide cyclase, (AOC) was analyzed in different developmental stages and various organs of Arabidopsis thaliana plants by immuno blot analysis and immunocytological approaches. Levels of AOC and of the two preceding enzymes in JA biosynthesis increased during seedling development accompanied by increased levels of JA and 12-oxophytodienoic acid levels after 4 and 8 weeks. Most tissues including all vascular bundles and that of flower buds contain AOC protein. Flowers shortly before opening, however, contain AOC protein preferentially in ovules, stigma cells and vascular bundles, whereas in anthers and pollen AOC could not be detected. The putative roles of AOC and JA in development are discussed.
authors.affiliation: Institute of Plant Biochemistry, Department of Secondary Metabolism, Weinberg 3, D-06120 Halle/S., Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Hause
authors.forename: Irene
authors.initials: I
authors.lastname: Stenzel
authors.forename: Otto
authors.initials: O
authors.lastname: Miersch
authors.forename: Claus
authors.initials: C
authors.lastname: Wasternack
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D015152:Blotting, Northern; D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008084:Lipoxygenase / Q000032:analysis / Q000378:metabolism; D054883:Oxylipins; D018514:Plant Structures / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014018:Tissue Distribution
chemical_list: D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D008084:Lipoxygenase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase