title: De novo synthesis of PCR templates for the development of SARS diagnostic assay.
abstract: A novel coronavirus was identified as the cause for severe acute respiratory syndrome (SARS). The complete sequence of SARS genome has provided an opportunity for the development of molecular diagnostic assays. To restrain further outbreak of SARS, the World Health Organization has posted several pairs of polymerase chain reaction (PCR) primers for early diagnosis and urged more research to be done on PCR protocols. Here we report a strategy for the de novo synthesis of PCR templates complimentary to the SARS virus genome, which has the advantage of working on PCR templates without concern about viral infection and also has the advantage that it can be used by those who do not have access to the SARS virus. This highly efficient and safe strategy for obtaining SARS gene fragments is useful for the development of PCR assays, as well as for the preparation of reliable positive controls for PCR testing kits.
authors.affiliation: Genomapping Inc., Tianjin.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Meng
authors.forename: Duanfang
authors.initials: D
authors.lastname: Liao
authors.forename: Lvyi
authors.initials: L
authors.lastname: Zhou
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Linling
authors.initials: L
authors.lastname: Chen
authors.forename: Zifen
authors.initials: Z
authors.lastname: Guo
authors.forename: Cuiying
authors.initials: C
authors.lastname: Peng
authors.forename: Bingyang
authors.initials: B
authors.lastname: Zhu
authors.forename: Peggy P
authors.initials: PP
authors.lastname: Lee
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Xu
authors.forename: Tianhong
authors.initials: T
authors.lastname: Zhou
authors.forename: Zemin
authors.initials: Z
authors.lastname: Deng
authors.forename: Yinghe
authors.initials: Y
authors.lastname: Hu
authors.forename: Kai
authors.initials: K
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D003955:Diagnostic Tests, Routine / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology; D013698:Templates, Genetic
chemical_list: D018076:DNA, Complementary