An improved preparation of bovine brain proteolipid.
An improved preparation of proteolipid from bovine brain white matter is described. The product obtained by repeated acetone precipitation is completely soluble in chloroform-methanol and has a fairly constant composition: 35% protein, 40% galactocerebroside, and about 25% phospholipid.
Cavanna
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Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA.
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D000096:Acetone; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D011232:Chemical Precipitation; D005699:Galactosylceramides / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification; D011510:Proteolipids / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility
D005699:Galactosylceramides; D009419:Nerve Tissue Proteins; D010743:Phospholipids; D011510:Proteolipids; D000096:Acetone