title: 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) for effective antisense formation.
abstract: ENA antisense oligonucleotides for vascular endothelial growth factor (VEGF) mRNA were synthesized and evaluated in A549 lung cancer cells. It was found that the VEGF ENA-antisense inhibited not only the expression of VEGF, but also the expression of three genes, which were found in Genbank by BLAST and Clustal W search and considered likely to bind to the VEGF ENA-antisense. These results indicate that ENA-antisense oligonucleotides act in a sequence-specific manner, and could be used as effective antisense drugs.
authors.affiliation: Exploratory Chemistry Research Laboratories, Sankyo Co., Limited, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Morita
authors.forename: Koji
authors.initials: K
authors.lastname: Yamate
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kurakata
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imanishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Koizumi
mesh_terms: D002460:Cell Line; D005030:Ethylenes; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007202:Indicators and Reagents; D008175:Lung Neoplasms; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D005030:Ethylenes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C036216:ethylene