Foreign gene delivery into monocotyledonous species.
Monocotyledonous plants are generally more recalcitrant to genetic transformation than dicotyledonous species. The absence of reliable Agrobacterium-mediated transformation methods and the difficulties associated with the culture of monocotyledonous tissues in vitro are mainly responsible for this situation. Until recently, the genetic transformation of monocotyledons was essentially performed by direct transfer of DNA into regenerable protoplasts or intact cells cultured in vitro, via polyethylene glycol treatment, electroporation or particle bombardment. Since 1990, the use of particle gun technology has revolutionized the genetic engineering of monocotyledonous species, allowing transformation to be more independent of the in vitro culture requirements. Today, at least one genotype of each major monocotyledonous crop species, including cereals, can be genetically transformed.
Vain
P
P
Institut de Biotechnologie des plantes, bat 630, URA CNRS 1128, Université Paris-Sud, 91405 Orsay, France.
De Buyser
J
J
Bui Trang
V
V
Haicour
R
R
Henry
Y
Y