title: [Effect of bone morphogenetic protein microspheres on biological behavior of rabbit bone marrow stem cells].
abstract: OBJECTIVE
  To evaluate the effect of bone morphogenetic protein (BMP) on the biological behavior of bone marrow stem cells (BMSCs) of rabbits.
  
  
  METHODS
  BMP was either enwrapped or not in the microspheres made of chitosan and sodium alginate, and the biocompatibilities of the composites were examined by means of cell culture. The BMSCs were cultured with the two kinds of microspheres respectively, and the cell extension rate, proliferation, alkaline phosphatase activity and Coomassie blue staining of the cells were assayed.
  
  
  RESULTS
  Inhibition of BMSC proliferation did not occur in response to in vitro culture with the microspheres, but alkaline phosphatase activity and D(lambda) values of Coomassie blue staining increased significantly in the cells cultured with BMP microspheres.
  
  
  CONCLUSIONS
  BMP can increase the osteogenic capacity of BMSCs in vitro with the microspheres made of chitosan and sodium alginate as the carrier.
authors.affiliation: Department of Traumatology, Nanfang Hospital, Guangzhou 510515, China. qinyu.fimmu@163.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Qin
authors.forename: Guo-xian
authors.initials: GX
authors.lastname: Pei
authors.forename: De-ming
authors.initials: DM
authors.lastname: Xie
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.forename: Kuan-hai
authors.initials: KH
authors.lastname: Wei
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division; D005260:Female; D008297:Male; D008863:Microspheres; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering
chemical_list: D019485:Bone Morphogenetic Proteins