Thymine at -5 is crucial for cpc promoter activity of Synechocystis sp. strain PCC 6714.
The levels of transcripts of the cpc operon were highly reduced in a PD-1 mutant of cyanobacterium Synechocystis sp. strain PCC 6714. This was due to a substitution of C for T that occurred at 5 bp upstream of the transcription initiation site of the cpc operon. Any substitution for T at the -5 position drastically reduced both in vivo and in vitro promoter activity in cyanobacterium Synechococcus sp. strain PCC 7942 but not the in vivo activity in Escherichia coli. This suggests that the requirement of -5T appears to be specific for a cyanobacterial RNA polymerase-promoter combination.
Imashimizu
Masahiko
M
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji 192-0392. CREST, Japan.
Fujiwara
Shoko
S
Tanigawa
Ryohei
R
Tanaka
Kan
K
Hirokawa
Takatsugu
T
Nakajima
Yuji
Y
Higo
Junichi
J
Tsuzuki
Mikio
M
D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009876:Operon; D010798:Phycocyanin / Q000235:genetics; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D013045:Species Specificity; D013941:Thymine / Q000737:chemistry; D014158:Transcription, Genetic
D010798:Phycocyanin; D012321:DNA-Directed RNA Polymerases; D013941:Thymine