Effects of "moxibustion serum" on proliferation and phenotypes of tumor infiltrating lymphocytes.
Tumor infiltrating lymphocytes (TIL) were cultured with "moxibustion serum" (MS), and the results were examined by flow cytometry. The results indicated that MS could enhance the proliferation of TIL, accelerate it to reach the exponential growth phase, and assist recombinant interleukin 2 (rIL-2) to enhance successively the percentage of CD3+ positive cells, maintain the number of CD4+ positive T cells, promote greatly the percentage of CD8+ positive T cells among TILs, and reverse the CD4+/CD8+ ratio. Such cooperative effects rely on relative specificity of acupoints. It is suggested that MS is beneficial to the growth of TIL both in the aspects of proliferation and phenotypes.
Chen
Yunfei
Y
Shanghai Institute of Acupuncture & Meridians, Shanghai 200030.
Zhao
Cuiying
C
Chen
Hanping
H
Qin
Huilian
H
Fang
Fang
F
D000818:Animals; D019306:Artemisia; D001769:Blood; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D002455:Cell Division / Q000187:drug effects; D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007376:Interleukin-2 / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009071:Moxibustion; D010641:Phenotype; D011994:Recombinant Proteins / Q000276:immunology; D013953:Thymus Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D017252:CD3 Complex; D017077:Culture Media, Conditioned; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D011994:Recombinant Proteins