Promoter activity and gene structure of rabbit FKBP52.
A 0.9-kb fragment situated directly upstream of the first ATG of rabbit FKBP52 is rich in acceptor sites for transcription factors, contains a CAAT box at -197 and could represent the proximal promoter of this immunophilin. Transvection analysis of this fragment showed strong promoter activity on the expression of a reporter gene. Deletions at the 5' end of this fragment showed that a basic sequence of 155 base pairs upstream of the CAAT box was sufficient to enhance luciferase expression an average 220-fold compared to the empty vector. This sequence, which contains acceptor sites for transcription factors of the EGR family and heat-shock factors, is closely homologous to 110 base pairs situated directly 5' of FKBP52 exon 1 in human chromosome 12p13.3, suggesting that these transcription factors could be involved in the regulation of the gene in both species. Furthermore, the upstream region of RbFKBP52 contains a large proportion of SINEs (C-repeats, Alu analogs), some of which include the A and B boxes required for transcription of RNA polymerase III, and poly A tracts. RbFKBP52, like HuFKBP52, is made up of 10 exons and 9 introns, a feature shared with other large immunophilins such as FKBP65 and Cyclophilin 40, and which appears widely conserved.
Massol
Nelly
N
INSERM U 488, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.
Lebeau
Marie-Claire
MC
Schumacher
Michael
M
Baulieu
Etienne-Emile
EE
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D005091:Exons; D007438:Introns; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011817:Rabbits; D022021:Tacrolimus Binding Proteins / Q000235:genetics*; D024363:Transcription Initiation Site
D020121:5' Untranslated Regions; D022021:Tacrolimus Binding Proteins