Detection of the free-living forms of sulfide-oxidizing gill endosymbionts in the lucinid habitat (Thalassia testudinum environment).
Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill endosymbiont of Codakia orbicularis is present in the environment as a free-living uncultivable form.
Gros
Olivier
O
Laboratoire de Biologie Marine, Département de Biologie, Université des Antilles et de la Guyane, 97159 Pointe-à-Pitre Cedex, Guadeloupe, France. olivier.gros@univ-ag.fr
Liberge
Martine
M
Heddi
Abdelaziz
A
Khatchadourian
Chaqué
C
Felbeck
Horst
H
D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D019015:Geologic Sediments; D005880:Gills / Q000382:microbiology*; D031321:Hydrocharitaceae / Q000254:growth & development*; D017404:In Situ Hybridization, Fluorescence; D008974:Mollusca / Q000382:microbiology*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012623:Seawater; D013440:Sulfides / Q000378:metabolism*; D013559:Symbiosis
D013440:Sulfides