Optical-transparent and flexible glucose sensor with ITO electrode.
The glucose sensor was constructed by immobilizing glucose oxidase (GOD) with glutaraldehyde solution onto the sensitive area of the transparent oxygen electrode. The oxygen electrode was fabricated by sealing KCl electrolyte solution including the Indium-Tin Oxide (ITO)-electrode with both metal-weldable film and gas-permeable membrane coated with Ag/AgCl electrode. The sensor behavior was evaluated using standard glucose solutions in a batch measurement system with a computer-controlled potentiostat at a reduction potential of -900 mV. The sensor device has flexible structure and good optical transparency (less than 0.6 abs) at the visible wavelength from 400 to 700 nm. The sensor was possible to be used for measuring glucose from 0.06 to 1.24 mmol/l (correlation coefficient: 0.999), including the reported concentration of tear glucose in normal (0.14 mmol/l), with good reproducibility.
Mitsubayashi
Kohji
K
Department of Human and Information Science, School of Engineering, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. mitsu@keyaki.cc.u-tokai.ac.jp
Wakabayashi
Yoshihiko
Y
Tanimoto
Satoshi
S
Murotomi
Daisuke
D
Endo
Tatsuro
T
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004548:Elasticity; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013666:Tears / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D017971:Tin Compounds; C109984:indium tin oxide; D005949:Glucose Oxidase; D005947:Glucose