Immunohistochemical distribution of surfactant apoprotein-A in congenital diaphragmatic hernia-II.
Morphometric analyses of the immunohistochemical expression of surfactant apoprotein A (SP-A) were carried out on the bronchioles of human congenital diaphragmatic hernia (CDH) neonates and then compared with those in a gestational and postnatal age-matched control group. There was no difference in SP-A expression between lung specimens of the control group and unaffected lungs in the CDH group. However, compared with both these lungs, the ipsilateral lungs of the CDH group showed a significant increase in SP-A expression, namely, the number of SP-A-positive cells per bronchiole, the number of SP-A-positive cells per unit perimeter of bronchiole, and the number of SP-A-positive cells per unit bronchiolar surface area. These results suggest that in lungs of CDH cases, especially ipsilateral lungs, there is a possible delay in the functional maturation or development of SP-A synthesis by the bronchiole, and this retardation may play a role in the postnatal respiratory insufficiency observed in CDH patients.
Asabe
Koushi
K
Department of Pediatric Surgery, Oita Prefectural Hospital, Oita, Japan. asabe@fukuoka-u.ac.jp
Tsuji
Koichi
K
Handa
Noritoshi
N
Kajiwara
Makoto
M
Suita
Sachiyo
S
D000704:Analysis of Variance; D015415:Biomarkers; D001707:Biopsy, Needle; D016022:Case-Control Studies; D005260:Female; D006548:Hernia, Diaphragmatic / Q000473:pathology*; D065630:Hernias, Diaphragmatic, Congenital; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008297:Male; D011336:Probability; D011379:Prognosis; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values; D018570:Risk Assessment
D015415:Biomarkers; D037662:Pulmonary Surfactant-Associated Protein A