[Effect of bone morphogenetic protein microspheres on biological behavior of rabbit bone marrow stem cells].
OBJECTIVE
To evaluate the effect of bone morphogenetic protein (BMP) on the biological behavior of bone marrow stem cells (BMSCs) of rabbits.


METHODS
BMP was either enwrapped or not in the microspheres made of chitosan and sodium alginate, and the biocompatibilities of the composites were examined by means of cell culture. The BMSCs were cultured with the two kinds of microspheres respectively, and the cell extension rate, proliferation, alkaline phosphatase activity and Coomassie blue staining of the cells were assayed.


RESULTS
Inhibition of BMSC proliferation did not occur in response to in vitro culture with the microspheres, but alkaline phosphatase activity and D(lambda) values of Coomassie blue staining increased significantly in the cells cultured with BMP microspheres.


CONCLUSIONS
BMP can increase the osteogenic capacity of BMSCs in vitro with the microspheres made of chitosan and sodium alginate as the carrier.
Qin
Yu
Y
Department of Traumatology, Nanfang Hospital, Guangzhou 510515, China. qinyu.fimmu@163.com
Pei
Guo-xian
GX
Xie
De-ming
DM
Jin
Dan
D
Wei
Kuan-hai
KH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002455:Cell Division; D005260:Female; D008297:Male; D008863:Microspheres; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering
D019485:Bone Morphogenetic Proteins