title: An integrated process for purification of lysozyme, ovalbumin, and ovomucoid from hen egg white.
abstract: This article describes an integrated process for simultaneous purification of lysozyme, ovalbumin, and ovomucoid from hen egg white. The crude egg white extract was passed through a cation exchanger Streamline trade mark SP and the bound lysozyme was eluted with 5% ammonium carbonate, pH 9.0, containing 1 M NaCl after elution of avidin. This partially purified lysozyme was further purified 639-fold on dye-linked cellulose beads. Ovalbumin and ovomucoid did not bind to Streamline SP. Ovalbumin could be precipitated from this unbound fraction by 5% trichloroacetic acid, and ovomucoid was removed from the supernatant by precipitation with ethanol. The yields of lysozyme, ovomucoid, and ovalbumin were 77, 94, and 98%, respectively. All the purified proteins showed single bands on sodium dodecyl sulfate polyacrylamide gel electrophoresis. All the steps are easily scalable, and the process described here can be used for large-scale simultaneous purification of these proteins in the pure form.
authors.affiliation: Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.forename: M V S
authors.initials: MV
authors.lastname: Rao
authors.forename: Munishwar N
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D000465:Algorithms; D000818:Animals; D002482:Cellulose; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004529:Egg White / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000302:isolation & purification*; D010047:Ovalbumin / Q000302:isolation & purification*; D010059:Ovomucin / Q000302:isolation & purification*
chemical_list: D004396:Coloring Agents; D010059:Ovomucin; D002482:Cellulose; D010047:Ovalbumin; D009113:Muramidase