title: A phytochemical study of lignans in whole plants and cell suspension cultures of Anthriscus sylvestris.
abstract: In the roots of Anthriscus sylvestris 12 different lignans were detected. Arctigenin, dimethylmatairesinol, dimethylthujaplicatin, podophyllotoxin, 7-hydroxyyatein and 7-hydroxyanhydropodorhizol have not been previously reported to be present in A. sylvestris. In the cell suspension cultures, which were initiated for this study, trace amounts of deoxypodophyllotoxin could be detected. With these cell suspension cultures we carried out feeding experiments using deoxypodophyllotoxin, yatein and, anhydropodorhizol. Yatein had a toxic effect on the cell cultures and was, like anhydropodorhizol, not converted into any detectable product. Deoxypodophyllotoxin, in contrast, was converted into podophyllotoxin, yielding significantly higher concentration than measured in whole plants.
authors.affiliation: Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), Groningen, The Netherlands.
authors.forename: Albert
authors.initials: A
authors.lastname: Koulman
authors.forename: Marlies E
authors.initials: ME
authors.lastname: Kubbinga
authors.forename: Sieb
authors.initials: S
authors.lastname: Batterman
authors.forename: Herman J
authors.initials: HJ
authors.lastname: Woerdenbag
authors.forename: Niesko
authors.initials: N
authors.lastname: Pras
authors.forename: Jack G
authors.initials: JG
authors.lastname: Woolley
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
mesh_terms: D019661:Apiaceae / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques; D002460:Cell Line; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017705:Lignans / Q000032:analysis / Q000096:biosynthesis*; D008517:Phytotherapy; D018514:Plant Structures / Q000166:cytology / Q000378:metabolism
chemical_list: D017705:Lignans