[Zygote formation in Blakeslea trispora: morphological peculiarities and relationship with carotenoid synthesis].
Changes associated with zygospore formation in the mucorous fungus Blakeslea trispora were studied. Zygospores are dormant cells with thickened cell walls and large central lipid vacuoles containing large amounts of lycopene. We established for the first time that B. trispora gametangia of different sexes differ in their carotenoid content and revealed that zygote formation involves a novel structure that consists of densely intertwined hyphae. Using inhibitory analysis (blocking beta-carotene synthesis with diphenylamine and 2-amino-6-methylpyridine), we showed that suppression of carotene production results in the inhibition of zygote formation. Hence, we established a manifest dependence of zygote formation on beta-carotene synthesis.
Tereshina
V M
VM
Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312 Russia.
Memorskaia
A S
AS
Feofilova
E P
EP
D002338:Carotenoids / Q000032:analysis; D025301:Hyphae / Q000166:cytology; D000077276:Lycopene; D008854:Microscopy, Electron; D009090:Mucorales / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013172:Spores, Fungal / Q000378:metabolism / Q000648:ultrastructure; D014617:Vacuoles / Q000737:chemistry / Q000378:metabolism; D015053:Zygote / Q000378:metabolism / Q000648:ultrastructure; D019207:beta Carotene / Q000096:biosynthesis*
D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene