title: Localisation of P2Y1 and P2Y4 receptors in dorsal root, nodose and trigeminal ganglia of the rat.
abstract: The presence and distribution of P2Y (nucleotide) receptor subtypes in rat sensory neurons has been investigated. RT-PCR showed that P2Y(1), P2Y(2), P2Y(4) and P2Y(6) receptor mRNA is expressed in sensory ganglia [dorsal root ganglion (DRG), nodose ganglion (NG) and trigeminal ganglion (TG)]. The regional and cellular distribution of P2Y(1) and P2Y(4) receptor proteins in these ganglia was investigated using immunohistochemistry. P2Y(1) polyclonal antibodies stained over 80% of the sensory neurons, particularly the small-diameter (neurofilament-negative) neurons. The P2Y(4) receptor antibody stained more medium- and large- (neurofilament-positive) diameter neurons than small-diameter neurons. P2Y(1) and P2Y(4) receptor immunoreactivity (P2Y(1)-IR and P2Y(4)-IR) was often coexpressed with P2X(3) receptor immunoreactivity (P2X(3)-IR) in subpopulations of neurons. Double immunohistochemistry showed that 73-84% of P2X(3) receptor-positive neurons also stained for the P2Y(1) receptor in DRG, TG and NG while only 25-35% also stained for the P2Y(4) receptor. Subpopulations of P2Y(1)-IR neurons were coexpressed with NF200, CGRP and IB(4); most P2Y(4)-IR neurons were coexpressed with NF200, while only a few neurons were coexpressed with CGRP (10-20%) or with IB(4) (1-2%). The results suggest that P2Y as well as P2X receptor subtypes contribute to purinergic signalling in sensory ganglia.
authors.affiliation: Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, NW3 2PF, London, UK.
authors.forename: Huai Zhen
authors.initials: HZ
authors.lastname: Ruan
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005727:Ganglia, Spinal / Q000737:chemistry / Q000502:physiology*; D015870:Gene Expression; D037102:Lectins; D016900:Neurofilament Proteins / Q000032:analysis; D009620:Nodose Ganglion / Q000737:chemistry / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000235:genetics*; D058477:Receptors, Purinergic P2X3; D058472:Receptors, Purinergic P2Y1; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000502:physiology*
chemical_list: D037102:Lectins; D016900:Neurofilament Proteins; C546410:P2rx3 protein, rat; C546384:P2ry1 protein, rat; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D058472:Receptors, Purinergic P2Y1; C104749:purinoceptor P2Y4; C051774:neurofilament protein H; D000255:Adenosine Triphosphate; D015740:Calcitonin Gene-Related Peptide