[Study of anti-breast cancer activity of tumor infiltrating lymphocytes affected by dendritic cells].
OBJECTIVE
Dendritic cell (DC) is the strongest antigen presenting cell(APC). It can present antigen to T lymphocytes in vivo and in vitro,and induce cytotoxic T lymphocyte(CTL) reactions.This study was designed to investigate the killing activity of tumor infiltrating lymphocytes (TILs) stimulated by dendritic cells on breast cancer cells in vitro.


METHODS
DCs were isolated from peripheral blood of patients with breast cancer. DCs were stimulated by granulocyte/macrophage colony stimulating factor (GM-CSF), interleukin-4(IL-4), and tumor antigen. Then TILs were stimulated by DCs and their killing activity on autogenous breast cancer cells and Bcap-37 breast cancer cells in vitro were observed.


RESULTS
TILs stimulated by DCs had very high killing activity on autogenous breast cancer cells and the killing rate was (85.76+/-2.93)%. The killing rate was higher obviously than that of TILs not stimulated by DCs and T lymphocytes stimulated by DCs or not on autogenous breast cancer cells, respectively [killing rates: (52.11+/-1.48)%, (51.35+/-1.46)%, and (3.59+/-0.25)%, respectively]. However, their killing activities on Bcap-37 breast cancer cells were lower [killing rates: (40.03+/-1.29)%, (22.09+/-0.87)%, (21.66+/-0.85)%, and (1.76+/-0.14)%, respectively].


CONCLUSIONS
The results indicate that DC from the patients with breast cancer can induce TIL to produce efficient and specific anti-breast cancer immune response.
Liu
Jian-Yong
JY
Department of Surgery, Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, PR China. jyliu99@hotmail.com
Zhang
Chun-Yan
CY
Zhao
Yin-Nong
YN
Tang
Kai
K
Zhang
Li-Tu
LT
Li
Ting
T
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000276:immunology*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008875:Middle Aged