Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene.
Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a method for identifying Bacillus anthracis by analyzing two chromosomal targets, the 16S-23S intergenic spacer region (ISR) and the gyrA gene. The 16S-23S ISR was analyzed by this method with 42 strains of B. anthracis, 36 strains of Bacillus cereus, and 12 strains of Bacillus thuringiensis; the gyrA gene was analyzed by this method with 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis. Two blind panels of 45 samples each were analyzed to evaluate the potential diagnostic capability of this method. Our results show that DHPLC is an efficient method for the identification of B. anthracis.
Hurtle
William
W
Clinical Research Management, North Royalton, Ohio 44133, USA.
Bode
Elizabeth
E
Kaplan
Rebecca Susan
RS
Garrison
Jeff
J
Kearney
Brian
B
Shoemaker
David
D
Henchal
Erik
E
Norwood
David
D
D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D027081:DNA Gyrase / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D027081:DNA Gyrase