title: Immunofluorescence stimulated emission depletion microscopy.
abstract: We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Göttingen, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Dyba
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000451:nursing*; D008870:Microtubules / Q000648:ultrastructure*; D012680:Sensitivity and Specificity