Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment.
Tissue engineering aims at resolving problems such as donor shortage and immune rejection faced by transplantation. Scaffolds (artificial extracellular matrices) have critical roles in tissue engineering. Recently, we developed nano-fibrous poly(L-lactic acid) scaffolds under the hypothesis that synthetic nano-fibrous scaffolding, mimicking the structure of natural collagen fibers, could create a more favorable microenvironment for cells. This is the first report that the nano-fibrous architecture built in three-dimensional scaffolds improved the features of protein adsorption, which mediates cell interactions with scaffolds. Scaffolds with nano-fibrous pore walls adsorbed four times more serum proteins than scaffolds with solid pore walls. More interestingly, the nano-fibrous architecture selectively enhanced protein adsorption including fibronectin and vitronectin, even though both scaffolds were made from the same poly(L-lactic acid) material. Furthermore, nano-fibrous scaffolds also allowed >1.7 times of osteoblastic cell attachment than scaffolds with solid pore walls. These results demonstrate that the biomimetic nano-fibrous architecture serves as superior scaffolding for tissue engineering.
Woo
Kyung Mi
KM
Department of Biologic and Materials Sciences, 1011 North University Avenue, Room 2211, The University of Michigan, Ann Arbor, Michigan 48109-1078, USA.
Chen
Victor J
VJ
Ma
Peter X
PX
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid; D010006:Osteoblasts / Q000502:physiology; D011108:Polymers; D044967:Serum / Q000378:metabolism
D001672:Biocompatible Materials; D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D011108:Polymers; D019344:Lactic Acid