[Enzyme activities and structural features of mitochondria and expression of mRNA of complex I].
The functional ability of the muscle is closely related to the activities of mitochondria, which are the energy producing organelles in the muscle cell. Complex I, III and IV exist as respiratory chain enzymes in the inner membrane of mitochondria and are responsible for transporting electrons from NADH to O2. In this paper, rat masseter muscle, rat and frog cerebral cortex, rat tongue, and frog tibialis anterior muscle mitochondria were described in terms of their correlation with enzyme activity of mitochondrial respiratory chain, the structure and number of mitochondria, and transcription levels of ND1 (mitochondrial) and 51K (nuclear) subunits of Complex I, especially regarding the change in feeding behavior in rat and hibernation in frog. The cristae in mitochondria gradually developed during the postnatal period. The changes in feeding behavior were closely correlated with the level of mitochondrial enzyme activity and were also supported by the structural changes in mitochondria. Based on results of this study, the expression of ND1 and 51K is thought to be involved in the rate-limiting step in the enzyme production. The results also suggest that there is a correlation between biochemical properties and structural features in mitochondria during development, effects of a soft diet, and hibernation.
Sato
Iwao
I
Department of Anatomy, School of Dentistry at Tokyo, Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.
D000818:Animals; D001001:Anura; D004579:Electron Transport / Q000502:physiology*; D042967:Electron Transport Complex I; D008928:Mitochondria / Q000000981:diagnostic imaging* / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D014463:Ultrasonography
D012333:RNA, Messenger; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I