Immobilization of animal cells in fixed bed bioreactors.
Immobilization of animal cells has become a highly popular means of achieving high-density animal cell cultures. The advantages of immobilization are that it stabilizes cells in culture and enables long-term culture periods to be achieved. Immobilization increases cell productivity by increasing the usable substrate surface area for anchorage-dependent cells, or by facilitating perfusion of anchorage-independent cells. A method for production of secreted biological products from anchorage-dependent and independent cells is described. The method is based on immobilization of animal cells within the polymeric matrix of polyurethane foam, packed in a fixed bed bioreactor.
Lazar
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Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 70450, Israel.