Heteroduplex mobility assay for the identification of Listeria sp and Listeria monocytogenes strains: application to characterisation of strains from sludge and food samples.
One hundred and ten Listeria sp. isolates from sewage sludge were identified according to phenotypic and genotypic methods. The Listeria sp. strains isolated from five types of sludge from three sewage treatment plants in Angers (France) and the surrounding area included L. monocytogenes (55.5%), L. innocua (29.1%), L. seeligeri (13.6%) and L. welshimeri (1.8%). The majority of L. monocytogenes strains belonged to serotypes 4b, 1/2b and 1/2a. Moreover, a heteroduplex mobility assay based on the 16S rRNA sequences was tested for its ability to identify the six species of the genus Listeria. This study, performed on 283 Listeria sp. strains from human, food and sewage sludge samples, showed that all the species were distinguishable from one another. L. innocua and L. seeligeri showed respectively three and two distinct banding patterns. Within L. monocytogenes, four groups (I-IV) were defined. The majority of food and environmental isolates were clustered in group I and it is noteworthy that group IV clustered epidemiologic isolates and strains belonging to serotypes 4b, 1/2a and 1/2b.
Garrec
N
N
Laboratoire des Sciences de l'Environnement et de l'Aménagement, 2 boulevard Lavoisier, 49045 Angers Cedex 2, France.
Marault
M
M
Kerouanton
A
A
Brisabois
A
A
Pourcher
A M
AM
Sutra
L
L
D000818:Animals; D001427:Bacterial Toxins; D015373:Bacterial Typing Techniques / Q000379:methods*; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D020459:Genes, rRNA; D006360:Heat-Shock Proteins / Q000235:genetics; D006460:Hemolysin Proteins; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D008087:Listeria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012703:Serotyping; D012722:Sewage / Q000382:microbiology*
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C038705:hlyA protein, Listeria monocytogenes