title: Renal renin-angiotensin system activity in naturally reared and cross-fostered spontaneously hypertensive rats.
abstract: BACKGROUND
  Young (4 week) spontaneously hypertensive rats (SHR) exhibit greater renal responses to angiotensin II (Ang II) than normotensive Wistar Kyoto (WKY) rats. SHR pups cross-fostering to a WKY dam at birth (SHRX) are less sensitive to Ang II and have lower adult blood pressure. The aim of this study was to compare renal renin-angiotensin system activity in young naturally reared and cross-fostered SHR pups.
  
  
  METHODS
  SHR and WKY rats were reared either by their natural mothers or by a foster mother of the opposite strain. At 5, 10, and 15 days of age, renal tissue renin activity and Ang II concentration were measured by radioimmunoassay. Renin-secreting cells were identified by in situ hybridization and AT(1) receptor expression was compared using Western blots. Ang II-mediated cAMP generation was measured in isolated proximal tubules.
  
  
  CONCLUSIONS
  Tissue renin activity and numbers of renin-secreting cells did not differ, but Ang II was higher in SHRX. The AT(1) receptor expression was significantly lower in SHRX compared with SHR. Basal and Ang II-stimulated cAMP was lower in SHR tubules compared with WKY and SHRX tubules.Cross-fostering reversed the increased renal sensitivity of the SHR to Ang II. These data suggest that renal AT(1) receptor expression can be manipulated during the postnatal period and that this may affect adult blood pressure.
authors.affiliation: School of Biological Sciences, University of Manchester, Manchester, UK.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Gouldsborough
authors.forename: George B M
authors.initials: GB
authors.lastname: Lindop
authors.forename: Nick
authors.initials: N
authors.lastname: Ashton
mesh_terms: D000804:Angiotensin II / Q000097:blood / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006977:Hypertension, Renal / Q000378:metabolism*; D017403:In Situ Hybridization; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System / Q000502:physiology*; D015290:Second Messenger Systems / Q000502:physiology; D012931:Social Environment; D013045:Species Specificity; D014662:Vasoconstrictor Agents / Q000097:blood / Q000494:pharmacology
chemical_list: D044140:Receptor, Angiotensin, Type 1; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000242:Cyclic AMP; D012083:Renin