title: CD40-targeted adenoviral GM-CSF gene transfer enhances and prolongs the maturation of human CML-derived dendritic cells upon cytokine deprivation.
abstract: Vaccination with autologous leukaemia-derived dendritic cells (DC) presents an adjuvant treatment option for chronic myeloid leukaemia (CML). Here, we show that high-efficiency CD40-targeted adenoviral gene transfer of GM-CSF to CML-derived DC induces long-lived maturation in the absence of exogenous cytokines and may thus ensure protracted stimulation of CML-specific T cells upon vaccination.
authors.affiliation: Department of Pathology, Free University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands.
authors.forename: A G M
authors.initials: AG
authors.lastname: Stam
authors.forename: S J A M
authors.initials: SJ
authors.lastname: Santegoets
authors.forename: T M
authors.initials: TM
authors.lastname: Westers
authors.forename: C C
authors.initials: CC
authors.lastname: Sombroek
authors.forename: J J W M
authors.initials: JJ
authors.lastname: Janssen
authors.forename: B W
authors.initials: BW
authors.lastname: Tillman
authors.forename: A A
authors.initials: AA
authors.lastname: van de Loosdrecht
authors.forename: H M
authors.initials: HM
authors.lastname: Pinedo
authors.forename: D T
authors.initials: DT
authors.lastname: Curiel
authors.forename: G J
authors.initials: GJ
authors.lastname: Ossenkoppele
authors.forename: R J
authors.initials: RJ
authors.lastname: Scheper
authors.forename: T D
authors.initials: TD
authors.lastname: de Gruijl
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D019013:CD40 Antigens / Q000235:genetics* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D019013:CD40 Antigens; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor