Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing.
A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up.
Nampalli
Satyam
S
Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA.
Finn
Patrick J
PJ
Sun
Lei
L
Xiao
Haiguang
H
Nelson
John R
JR
Grossmann
Gregory
G
Flick
Parke K
PK
Fuller
Carl W
CW
Kumar
Shiv
S
D001483:Base Sequence; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis*; D015224:Dideoxynucleosides / Q000138:chemical synthesis*; D004735:Energy Transfer; D007202:Indicators and Reagents
D004396:Coloring Agents; D003854:Deoxyribonucleotides; D015224:Dideoxynucleosides; D007202:Indicators and Reagents; D004247:DNA