title: Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.
abstract: [reaction: see text] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.
authors.affiliation: Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbot.com
authors.forename: Maciej
authors.initials: M
authors.lastname: Adamczyk
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Mattingly
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Moore
authors.forename: You
authors.initials: Y
authors.lastname: Pan