Exploratory and confirmatory gene expression profiling of mac1Delta.
Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Delta. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1.
De Freitas
Jeane Maria
JM
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA.
Kim
Jeung Hyoun
JH
Poynton
Helen
H
Su
Trent
T
Wintz
Henri
H
Fox
Tama
T
Holman
Patty
P
Loguinov
Alex
A
Keles
Sunduz
S
van der Laan
Mark
M
Vulpe
Chris
C
D001483:Base Sequence; D003300:Copper / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D007501:Iron / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; C084954:MAC1 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper; D007501:Iron