title: Development and validation of a liquid chromatographic method for monitoring of process-related synthetic organic impurities of profenofos in technical products.
abstract: A simple and rapid reversed-phase high-performance liquid chromatographic method for the monitoring of process-related synthetic organic impurities of profenofos (PFS) is developed. Impurities are separated and determined on a reversed-phase Hypersil C(18) column using gradient elution of 50 mM ammonium formate buffer-acetonitrile as a mobile phase and detection at 230 nm at ambient temperature. The method is validated with respect to accuracy, precision, linearity, and limits of detection and quantitation. The method is found to be suitable not only for monitoring the reactions involved in the process development of PFS, but also quality assurance, as it can detect impurities at the level of 1.5 x 10(-8) g.
authors.affiliation: HPLC/UV Group, Analytical Chemistry Division, Indian Institute of Chemical Technology, Uppal Road, Hyderabad-500 007, India.
authors.forename: R
authors.initials: R
authors.lastname: Nageswara Rao
authors.forename: D
authors.initials: D
authors.lastname: Naga Raju
authors.forename: N
authors.initials: N
authors.lastname: Venkateswarlu
authors.forename: B
authors.initials: B
authors.lastname: Vittal Rao
authors.forename: N
authors.initials: N
authors.lastname: Parvathi
authors.forename: A
authors.initials: A
authors.lastname: Manjula
authors.forename: G N
authors.initials: GN
authors.lastname: Reddy
authors.forename: P B
authors.initials: PB
authors.lastname: Gawali
authors.forename: M
authors.initials: M
authors.lastname: Sreekanth
authors.forename: P
authors.initials: P
authors.lastname: Nageswara Rao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007306:Insecticides / Q000737:chemistry*; D009930:Organic Chemicals / Q000032:analysis*; D063086:Organothiophosphates / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007306:Insecticides; D009930:Organic Chemicals; D063086:Organothiophosphates; C024273:profenofos