[Intensive drying of grids after lead citrate staining lowers precipitate contamination on the surface of ultrathin sections].
Two techniques are proposed to minimize precipitate contamination of ultrathin sections after lead citrate staining. According to the first one, stained and washed grids are placed, sections downward, on a stack of filter papers. The second one involves a consecutive washing of grids with distilled water, 96% ethanol, and n-hexane. Both techniques are equally efficient; the former being simpler in processing, while the latter is superior in the reproducibility of results.
Khokhlov
S E
SE
Central Research Laboratory, St. Petersburg State Medical University, St. Petersburg.
Nevorotin
A I
AI
D019343:Citric Acid; D007854:Lead; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*
D019343:Citric Acid; D007854:Lead