title: Fructose-fed rats are protected against ischemia/reperfusion injury.
abstract: This study examines the relationship between insulin resistance (IR) induced by fructose feeding (FF) and susceptibility to myocardial ischemia/reperfusion injury (MI/R). Six-week-old male Sprague-Dawley rats were randomized into control (CON; n = 59) or FF (n = 58) groups. After 4 weeks, rats were further randomized into one of the following groups: placebo, ischemic preconditioning (IPC), 5-hydroxydecanoic acid (5-HD) (10 mg/kg), or 5-HD + IPC. Moreover, to determine the role of fructose, a second model of IR (Zucker obese) and rats fed fructose diet for 3 days (FF-3) were also subjected to MI/R. In all experiments, rats were subjected to 30 min of myocardial ischemia and 4 h of reperfusion. In rats randomized to placebo, infarct size was significantly reduced by FF (24 +/- 5%) compared with CON (54 +/- 1%, p < 0.05). Pretreatment with 5-HD did not alter the infarct size in CON (45 +/- 5%) but inhibited the protection afforded by FF (53 +/- 7%). IPC reduced the infarct size to an equivalent level in both groups, whereas 5-HD administration prior to IPC blunted the IPC effect. In Zucker obese rats, infarct size was significantly larger (57 +/- 4%) compared with lean controls (37 +/- 4%, p < 0.05). In FF-3 rats, infarct size was also decreased (20 +/- 2%, p < 0.01) compared with CON. This study suggests that fructose feeding affords protection against MI/R that is related to or mimics preconditioning. This protection is not consistent with other models of IR and is likely related to the fructose diet itself.
authors.affiliation: Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Hanes 1050, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Jordan
authors.forename: Steven A
authors.initials: SA
authors.lastname: Simandle
authors.forename: Christina D
authors.initials: CD
authors.lastname: Tulbert
authors.forename: David W
authors.initials: DW
authors.lastname: Busija
authors.forename: Allison W
authors.initials: AW
authors.lastname: Miller
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004032:Diet; D005632:Fructose / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011924:Rats, Zucker
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005632:Fructose; D009195:Peroxidase