Reactive gliosis in areas around third ventricle in association with epileptogenesis in amygdaloid-kindled rat.
Repeated focal electrical stimulation of the brain is known to produce epileptogenesis, and this phenomenon is recognized as kindling. It is also considered to be a model of temporal lobe epilepsy. In the present study, morphological changes in areas around third ventricle in rat brain were examined according to the progression of kindling stage. Very few Glial Fibrillary Acidic Protein (GFAP)-positive astrocytes were present in ependymal cell layer at C0 stage. However, there was a specific increase in GFAP-positive cells in ependymal cell layer at stage C3 as compared to stage C0. Furthermore, GFAP-positive cells showed migration to subependymal zone (SEZ). By stage C5, almost all GFAP-positive cells had migrated to SEZ. While the precise mechanism of this cell migration is not clear, the results suggest a relationship between progression of kindling stage and astrogliosis.
Miyazaki
Tetsuji
T
Department of Perinatology and Gynecology, Kagawa Medical University, Miki, Kagawa 761-0793, Japan.
Miyamoto
Osamu
O
Janjua
Najma A
NA
Hata
Toshiyuki
T
Takahashi
Fumio
F
Itano
Toshifumi
T
D000679:Amygdala / Q000528:radiation effects; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000503:physiopathology / Q000528:radiation effects; D002452:Cell Count; D016923:Cell Death / Q000502:physiology; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods; D004805:Ependyma / Q000378:metabolism / Q000503:physiopathology; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000503:physiopathology*; D005904:Glial Fibrillary Acidic Protein; D005911:Gliosis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D007696:Kindling, Neurologic; D008297:Male; D009457:Neuroglia / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020542:Third Ventricle / Q000166:cytology / Q000503:physiopathology*
D005904:Glial Fibrillary Acidic Protein