[Change of the erythrocyte chemokine receptor binding activity in scalded rats with Pseudomonas aeruginosa infection].
OBJECTIVE
To explore the influence of infection on the erythrocyte chemokine receptor (ECKR) binding activity in severely scalded rats.


METHODS
The Sprague-Dawley (SD) rats were randomly divided into three group: sham scald group (A), burn and infection group (B) and infection group (C). The B group rats were scalded with 30% total body surface area (TBSA) of III degree, and the rats ECKR binding activity with interleukin-8 (IL-8) as ligand were detected by enzyme-linked immunosorbent assay (ELISA) at 1, 2, 4, 6 and 8 hours after infection.


RESULTS
Compared to that in A group, ECKR binding activities declined significantly (both P<0.01) after infection in both of B and C groups, but they increased at 8 hours (P<0.01). ECKR binding activity in B group was significantly less at 2, 8 hours than that in C group after infection (both P<0.05). The declining range of ECKR binding activity was more in B group, in which the decline of ECKR binding activity appeared earlier (2 hours) than that in C (6 hours) group.


CONCLUSIONS
The infection lead to the decline of ECKR binding activity in burned rats, and the erythrocytes might participate in the chemokine regulation and play a novel role in the infection.
Chen
Xiao-dong
XD
Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China.
Wang
Shun-bin
SB
Wu
Bo-yu
BY
Jiang
Qiong
Q
D000818:Animals; D002056:Burns / Q000097:blood / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D011552:Pseudomonas Infections / Q000097:blood / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019707:Receptors, Chemokine / Q000097:blood*; D013997:Time Factors
D019707:Receptors, Chemokine