Suppression of ethanol and lipopolysaccharide-induced liver injury by extracts of Hydrangeae Dulcis Folium in rats.
In female SD rats that were injected with 4 g/kg BW ethanol p.o. followed by a 5 mg/kg BW lipopolysaccharide (LPS) i.v. injection, serum glutamic pyruvic transaminases (GPT) activity increased to about eight times that of normal rats. In this model, rats that had been fed a diet containing 1% Hydrangeae Dulcis Folium (HDF) extracts for fifteen days showed significantly lower serum GPT activity (380.0+/-58.2 IU/l) than the control group (3527.0+/-774.1 IU/l). HDF's efficacy was far superior to milk thistle in this model (2950.0+/-915.9 IU/l). When mouse macrophages were treated with HDF extracts at 50 microg/ml, TNF-alpha production induced by LPS was suppressed to about 10% of the control. Rat serum TNF-alpha levels induced by LPS was decreased to 58.7% of the control by administering 1000 mg/kg BW HDF extract p.o. These results indicate that HDF prevents alcohol-induced liver injury through the inhibition of TNF-alpha production.
Hashizume
Erika
E
Kyowa Hakko Kogyo Co., LTD. Tsukuba Research Laboratories, Ibaraki Prefecture, Japan. erika.hashizume@kyowa.co.jp
Nakagiri
Ryusuke
R
Shirai
Akio
A
Kayahashi
Shun
S
Yasushi
Sakai
S
Kamiya
Toshikazu
T
D000410:Alanine Transaminase / Q000097:blood; D000821:Animal Feed; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005260:Female; D029628:Hydrangeaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D020944:Silybum marianum / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; D000410:Alanine Transaminase