title: In vivo lipoprotein binding assay of the insect exchangeable apolipoprotein, apolipophorin-III.
abstract: An original method for the study of the lipid binding properties of exchangeable apolipoproteins is reported. Binding of Locusta migratoria apolipophorin-III to Manduca sexta low-density lipophorin (LDLp) and high-density lipophorin (HDLp) was studied in vivo. This assay could be used useful to investigate the effect of mutations in the lipid binding properties of exchangeable apolipoproteins under physiological conditions.
authors.affiliation: 147 Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. jose@biochem.okstate.edu
authors.forename: Palaniappan S
authors.initials: PS
authors.lastname: Chetty
authors.forename: Estela L
authors.initials: EL
authors.lastname: Arrese
authors.forename: Jose L
authors.initials: JL
authors.lastname: Soulages
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006110:Grasshoppers; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D018402:Manduca; D014461:Ultracentrifugation
chemical_list: D001053:Apolipoproteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C042470:apolipophorin III; C032830:lipophorin