Diagnosis of trichomoniasis in 'virgin' bulls by culture and polymerase chain reaction.
The diagnostic test for Tritrichomonas foetus in bulls is microscopic examination of cultured preputial samples. Trichomonads other than T. foetus can be present in a preputial sample. Both a staining technique and a polymerase chain reaction assay were useful in differentiating between T. foetus and another trichomonad observed in samples from virgin bulls.
Parker
Sarah
S
Centre for Animal Parasitology, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Saskatchewan S7N 2R3.
Campbell
John
J
McIntosh
Kathy
K
Gajadhar
Alvin
A
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003937:Diagnosis, Differential; D005837:Genitalia, Male / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity; D013194:Staining and Labeling / Q000662:veterinary; D014244:Trichomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014245:Trichomonas Infections / Q000175:diagnosis / Q000662:veterinary*