Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease.
The major challenge in allogeneic hematopoietic cell transplantation is how to transfer allogeneic T-cell immunity without causing graft-versus-host disease (GVHD). Here we report a novel strategy to selectively prevent GVHD by depleting CD62L(+) T cells (naive and a subset of memory T cells). In unprimed mice, CD62L(-) T cells (a subset of memory T cells) failed to proliferate in response to alloantigens (which the mice have never previously encountered) and were unable to induce GVHD in allogeneic hosts. CD62L(-) T cells contributed to T-cell reconstitution by peripheral expansion as well as by promoting T-cell regeneration from bone marrow stem/progenitor cells. CD62L(-) T cells from the animals previously primed with a tumor cell line (BCL1) were able to inhibit the tumor growth in vivo but were unable to induce GVHD in the third-party recipients. This novel technology may allow transfer of allogeneic recall antitumor and antimicrobial immunity without causing GVHD.
Chen
Benny J
BJ
Bone Marrow Transplantation Program, Department of Medicine, Human Vaccine Institute, Duke University Medical Center, 2400 Pratt St, Ste 1100, Box 3961, Durham, NC 27705, USA.
Cui
Xiuyu
X
Sempowski
Gregory D
GD
Liu
Congxiao
C
Chao
Nelson J
NJ
D019264:Adoptive Transfer; D000818:Animals; D006086:Graft vs Host Disease / Q000276:immunology*; D019337:Hematologic Neoplasms / Q000276:immunology* / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D007156:Immunologic Memory / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D019041:L-Selectin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation*
D007519:Isoantigens; D019041:L-Selectin