Structure elucidation of Sch 538415, a novel acyl carrier protein synthase inhibitor from a microorganism.
A novel acyl carrier protein synthase inhibitor, Sch 538415 (1), was isolated from an unidentified bacterial microbe. Structure elucidation of 1 was accomplished based on analysis of spectroscopic data including UV, MS and 2D-NMR spectra. Compound 1 exhibited inhibitory activity in the acyl carrier protein synthase (AcpS) assay with an IC(50) value of 4.19 microM and showed antibacterial activity against Staphylococcus aureus in the agar diffusion assay.
Chu
Min
M
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. min.chu@spcorp.com
Mierzwa
Ronald
R
Xu
Ling
L
Yang
Shu-Wei
SW
He
Ling
L
Patel
Mahesh
M
Stafford
Jill
J
Macinga
David
D
Black
Todd
T
Chan
Tze-Ming
TM
Gullo
Vincent
V
D001372:Aza Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D001419:Bacteria / Q000737:chemistry; D002853:Chromatography, Liquid; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008970:Molecular Weight; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011809:Quinones / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship; D017855:Transferases (Other Substituted Phosphate Groups) / Q000037:antagonists & inhibitors*
D001372:Aza Compounds; D011500:Protein Synthesis Inhibitors; D011809:Quinones; C486843:Sch 538415; D017855:Transferases (Other Substituted Phosphate Groups); C021364:holo-(acyl-carrier-protein) synthase