Isolation and identification of an antioxidant enzyme catalase stimulatory compound from Garnoderma lucidum.
Antioxidant enzymes are scavenger reactive-oxygen intermediates and are involved in many cellular defense systems. We previously reported that a crude extract of Garnoderma lucidum, a medicinally potent mushroom, profoundly increased the catalase gene expression and enzyme activities in mouse livers (Park et al., J. Biochem. Mol. Biol. 34. 144-149, 2001). In this study, we elucidated the detailed mechanism whereby G. lucidum stimulates the catalase activity and expression. The major active fraction was isolated from G. lucidum and methyl linoleate was considered the most major component of the fraction. In order to determine whether methyl linoleate increases mRNA and protein synthesis of catalase, Northern and Western blot analyses were performed in vivo with methyl linoleate-treated mouse liver homogenate after feeding methyl linoleate to the mice. Northern and Western blot analyses of the crude liver homogenates in the mice that were administered methyl linoleate revealed that the expression catalase was significantly increased when compared to the untreated controls. In addition, the catalase protein levels and enzymatic activities increased in the mouse liver homogenates. These results suggest that methyl linoleate that is produced by G. lucidum stimulates the catalase expression at the transcription level.
Lee
Hyeon Yong
HY
Division of Life Sciences, Hallym University, Chunchon 200-702, Korea.
Eum
Won Sik
WS
Kim
Dae Won
DW
Lee
Byung Ryong
BR
Yoon
Chang Sik
CS
Jang
Sang Ho
SH
Choi
Hee Soon
HS
Choi
Soo Hyun
SH
Baek
Nam-In
NI
Kang
Jung Hoon
JH
Kang
Tae-Cheon
TC
Won
Moo Ho
MH
Cho
Sung-Woo
SW
Lee
Kil Soo
KS
Park
Jinseu
J
Choi
Soo Young
SY
D000363:Agaricales / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D020536:Enzyme Activators / Q000494:pharmacology*; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D008041:Linoleic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D020536:Enzyme Activators; D008041:Linoleic Acids; C005575:methyl linoleate; D002374:Catalase