Monitoring sweat calcium using skin patches.
The purpose of this study was to determine whether a simple noninvasive sweat collection method using skin patches would be useful in monitoring sweat Ca and to determine changes in dermal Ca loss during a bed rest study testing a resistive exercise countermeasure. The study showed that the technique was highly reproducible: the mean intra-subject variation approached zero and the inter-individual variability (%CV) varied from 18% to 32% for the three anatomical regions (arm, chest, and back) tested. There was less than 10% difference in sweat Ca excretion from different skin regions within the same individual at a given time point. A calculated estimate of total body sweat excretion for 12 bed rest subjects was 35 +/- 4 mg/day (mean +/- SE), close to published whole body measurements. Bed rest testing showed no significant differences with or without exercise when conducted in a temperature-controlled environment. We conclude that the skin patch technique is useful for monitoring changes in sweat Ca.
Rianon
N
N
Baylor College of Medicine, Houston, Texas, USA.
Feeback
D
D
Wood
R
R
Driscoll
T
T
Shackelford
L
L
LeBlanc
A
A
D000328:Adult; D001510:Bed Rest; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D015444:Exercise / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods*; D013542:Sweat / Q000737:chemistry / Q000378:metabolism*; D014870:Water Loss, Insensible / Q000502:physiology
D002118:Calcium