title: Transformation of the host-selective toxin destruxin B by wild crucifers: probing a detoxification pathway.
abstract: The destruxin B detoxification pathway present in Sinapis alba is also present in three unrelated species, Camelina sativa, Capsella bursa-pastoris, and Eruca sativa, suggesting a conservation of this pathway across crucifers. The chemical structure of a destruxin B metabolite, (6'-O-malonyl)hydroxydestruxin B beta-D-glucopyranoside, was also establised. Considering that Camelina sativa and Capsella bursa-pastoris detoxify destruxin B and produce the phytoalexins camalexins, these wild crucifers appear to represent unique and perhaps useful sources of blackleg resistance in strategic plant breeding.
authors.affiliation: Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK, Canada S7N 5C9. soledade.pedras@usask.ca
authors.forename: M S C
authors.initials: MS
authors.lastname: Pedras
authors.forename: S
authors.initials: S
authors.lastname: Montaut
authors.forename: I L
authors.initials: IL
authors.lastname: Zaharia
authors.forename: Y
authors.initials: Y
authors.lastname: Gai
authors.forename: D E
authors.initials: DE
authors.lastname: Ward
mesh_terms: D001711:Biotransformation; D019607:Brassicaceae / Q000378:metabolism*; D002250:Carbon Radioisotopes; D047630:Depsipeptides; D005960:Glucosides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D006900:Hydroxylation; D015394:Molecular Structure; D009183:Mycotoxins / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D012717:Sesquiterpenes; D013729:Terpenes; D013997:Time Factors; D000094162:Phytoalexins
chemical_list: D002250:Carbon Radioisotopes; D047630:Depsipeptides; D005960:Glucosides; D009183:Mycotoxins; D010456:Peptides, Cyclic; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C040424:destruxin B; D000094162:Phytoalexins