Preliminary 2-D chromatographic investigation of the human stem cell proteome.
Stem cells represent a promising tool for the treatment of various hematopoietic diseases. In order to identify stem cell-specific proteins, the proteome of human stem cells from umbilical cord blood was explored for the first time. For this purpose, the crude lysate of 4 x 10(5) CD34+ cells was subjected to in solution trypsin digestion. The resulting peptides were then separated via cation exchange followed by reversed phase chromatography and analyzed by nanospray MS/MS. Database search revealed a total of 215 proteins which could be reliably identified. To obtain a more complete picture of the human stell cell proteome and to also access low abundant proteins, pooling of more than one CD34+ preparations seems necessary in order to increase the cell number and thus the protein content.
Zenzmaier
Christoph
C
Lifecord Inc., Graz, Austria.
Kollroser
Manfred
M
Gesslbauer
Bernd
B
Jandrositz
Anita
A
Preisegger
Karl-Heinz
KH
Kungl
Andreas J
AJ
D018952:Antigens, CD34 / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D020543:Proteome / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D013234:Stem Cells / Q000737:chemistry*
D018952:Antigens, CD34; D020543:Proteome