Localization of the P2Y4 receptor in the guinea pig organ of Corti.
Cochleae of guinea pigs were evaluated for the presence of the metabotropic receptor, P2Y4. Evidence is presented that P2Y4 protein is expressed in the guinea pig cochleae using Western blot analysis. A single protein band of 35 kDa was detected with P2Y4 receptor-specific antibody. The cellular distribution of P2Y4 purinoceptor protein was determined by immunohistochemistry of the whole organ of Corti. Immunoreactive staining for P2Y4 was seen in most cells of the organ of Corti. Staining of Hensen's cells and Deiters' cells, especially the outer Deiters' cells, was more intense than staining of the outer hair cells, inner hair cells, and pillar cells. Staining intensity was greatest at the basal turn and progressively decreased in the upper turns with the apex showing the weakest staining pattern. This is the first demonstration of a metabotropic P2Y receptor in the guinea pig organ of Corti.
Parker
Margarett S
MS
Kresge Hearing Research Laboratory, Department of Otorhinolaryngology and Head and Neck Surgery, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA.
Onyenekwu
Nkeiruka N
NN
Bobbin
Richard P
RP
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D006168:Guinea Pigs; D007150:Immunohistochemistry; D018377:Neurotransmitter Agents / Q000032:analysis; D009925:Organ of Corti / Q000737:chemistry*; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2
D018377:Neurotransmitter Agents; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2; C104749:purinoceptor P2Y4; D000255:Adenosine Triphosphate