Single immunization of newborn mice with heterologous type-II collagen induces arthritic disease.
Collagen-induced arthritis (CIA) is a widely accepted model of autoimmune disease with significant similarities to rheumatoid arthritis in humans. CIA is provoked in susceptible strains upon immunization of adult mice with native type-II collagen in complete Freund's adjuvant (CFA). Neonatal exposure to antigen is supposed to result in T cell clone deletion and induction of tolerance. Here we report that the neonatal injection of bovine type-II collagen (bCII) to ICR (CD-2) mice triggers the development of autoimmune chronic joint inflammation. Compared with standard CIA significant joint swelling was not observed and anti-collagen antibodies were not detected if the second challenge with the antigen was not supplied. Histopathologic examination of the joints showed cell infiltration, synovial hyperplasia and at the later period bone destruction. Mice immunized as neonates expressed Ag-specific proliferative response and delayed type hypersensitivity (DTH) reaction to bCII.
Ivanovska
Nina
N
Department of Immunology, Institute of Microbiology, 26 G. Bonchev Street, 1113 Sofia, Bulgaria. nina@microbio.bas.bg
Yordanov
Martin
M
Raykovska
Vessela
V
D000818:Animals; D000831:Animals, Newborn; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000276:immunology; D024043:Collagen Type II / Q000276:immunology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006651:Histocytochemistry; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D024043:Collagen Type II; D007074:Immunoglobulin G