title: Protein phosphorylation in microorganisms.
abstract: The covalent modification of proteins by phosphorylation constitutes a major regulatory mechanism. It was first recognized in mammalian tissues. A conclusive evidence for the occurrence of protein phosphorylation and protein kinases in coliform bacteria was obtained in 1978. Several phosphate labeled proteins were found when Salmonella typhimurium was pulse-labeled with 32p(i) and solubilized bacterial contents were analyzed by SDS-polyacrylamide gel electrophoresis. In streptomycetes protein phosphorylation has not yet been demonstrated. We found that Streptomyces albus possesses a protein kinase activity. This in vitro protein phosphorylation is cAMP-independent.
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia.
authors.forename: J
authors.initials: J
authors.lastname: Janecek
authors.forename: Z
authors.initials: Z
authors.lastname: Dobrová