title: Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola.
abstract: longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing,allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter,and trans-spliced to the constant exons transcribed separately.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Horiuchi
authors.forename: Edward
authors.initials: E
authors.lastname: Giniger
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; C121765:lola protein, Drosophila