Linear polyethylenimine as a tool for comparative studies of antisense and short double-stranded RNA oligonucleotides.
Despite the recently enlarged field of available RNA knock-down technologies, e.g., antisense oligonucleotides (ASOs) and duplexes of synthetic 21 nucleotides RNAs (siRNAs), no versatile transfection reagent has been reported to deliver different nucleic acids formats at high rates of efficiency. We have evaluated the versatility and efficacy of linear PEI in transfecting and properly delivering a broad panel of nucleic acids such as short oligonucleotides and double-stranded RNA into cells in culture.
Bologna
Jean-Charles
JC
Functional Genomics, Novartis Pharma AG, Basel, Switzerland.
Dorn
Gabriele
G
Natt
Francois
F
Weiler
Jan
J
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D007202:Indicators and Reagents; D011094:Polyethyleneimine / Q000494:pharmacology*; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D016372:RNA, Antisense / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000235:genetics; D058477:Receptors, Purinergic P2X3; D014162:Transfection
D007202:Indicators and Reagents; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D012313:RNA; D011094:Polyethyleneimine