title: Rapid detection of duplication/deletion of the PMP22 gene in patients with Charcot-Marie-Tooth disease Type 1A and hereditary neuropathy with liability to pressure palsy by real-time quantitative PCR using SYBR Green I dye.
abstract: Mutations and altered gene dosage of the peripheral myelin protein (PMP22) gene in chromosome 17p11.2-12 are the main causes for hereditary neuropathies, accounting for approximately 70% of all cases. Patients with duplication of the PMP22 develop Charcot-Marie-Tooth disease type 1A (CMT1A) and deletion of one PMP22 allele leads to hereditary neuropathy with liability to pressure palsy (HNPP). Twenty patients with CMT1A, 17 patients with HNPP, and 18 normal family members and 28 normal controls were studied by real-time quantitative PCR using SYBR Green I on the ABI 7700 Sequence Detection System. The copy number of the PMP22 gene was determined by the comparative threshold cycle method and the albumin was used as a reference gene. The PMP22 duplication ratio ranged from 1.45 to 2.06 and the PMP22 deletion ratio ranged from 0.42 to 0.64. The PMP22 ratio in normal controls, including normal family members, ranged from 0.85 to 1.26. No overlap was found between patients with CMT1A or patients with HNPP and normal controls. This method is fast, highly sensitive, specific, and reproducible in detecting PMP22 duplication and deletion in CMT1A and HNPP patients, respectively.
authors.affiliation: Division of Genetic Disease, Department of Biomedical Science, National Institute of Health, Seoul, Korea.
authors.forename: Sang-Wun
authors.initials: SW
authors.lastname: Kim
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Hyun-Seok
authors.initials: HS
authors.lastname: Jin
authors.forename: Tae-Mi
authors.initials: TM
authors.lastname: Lee
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Koo
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Lee
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Jung
mesh_terms: D052160:Benzothiazoles; D002607:Charcot-Marie-Tooth Disease / Q000175:diagnosis* / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D003959:Diamines; D005192:Family Health; D005260:Female; D005456:Fluorescent Dyes / Q000494:pharmacology*; D017353:Gene Deletion; D020440:Gene Duplication; D015417:Hereditary Sensory and Motor Neuropathy / Q000235:genetics*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D009930:Organic Chemicals / Q000494:pharmacology*; D010243:Paralysis / Q000235:genetics*; D010523:Peripheral Nervous System Diseases / Q000235:genetics*; D011804:Quinolines; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D009930:Organic Chemicals; C489785:PXMP2 protein, human; D011804:Quinolines; C098022:SYBR Green I