title: Environmental oxygen tension affects phenotype in cultured bone marrow-derived macrophages.
abstract: This study tested the hypothesis that the unique phenotype of alveolar macrophages (AM) is maintained through adaptation to the relatively high oxygen partial pressure (P(O2)) of the lung, through modification of redox-sensitive transcription factors. BALB/c mouse bone marrow-derived macrophages (BMC) were differentiated under different P(O2) and compared functionally to AM and peritoneal macrophages (PM). BMC differentiated in normoxia (P(O2) 140 Torr, BMC(high)) were similar to AM in having low phagocytic and antigen presenting cell (APC) activities. However, BMC grown in low oxygen tension as found in other tissues (<40 Torr, BMC(low)) were better phagocytes and APCs, similar to PM. BMC(high) were more oxidative intracellularly than BMC(low), based on oxidation of dichlorofluorescein and higher glutathione disulfide/glutathione (GSH) ratios, despite having more GSH. Finally, lipopolysaccharide-induced nuclear factor-kappaB translocation, measured by laser scanning cytometry, was reduced in BMC(high) and AM, compared with BMC(low) and PM, respectively. These data suggest that regulation of the AM phenotype may occur, at least in part, via inhibition of NF-kappaB by the unique redox environment.
authors.affiliation: Center for Environmental Health Sciences, University of Montana, Missoula, MT 59812, USA. jpfau@selway.umt.edu
authors.forename: Jean C
authors.initials: JC
authors.lastname: Pfau
authors.forename: Jordan C
authors.initials: JC
authors.lastname: Schneider
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Archer
authors.forename: Jami
authors.initials: J
authors.lastname: Sentissi
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Leyva
authors.forename: Jennifer
authors.initials: J
authors.lastname: Cramton
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D010641:Phenotype; D011014:Pneumonia / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen