Development of the larval nervous system of the gastropod Ilyanassa obsoleta.
Gastropods have been well studied in terms of early cell cleavage patterns and the neural basis of adult behaviors; however, much less is known about neural development in this taxon. Here we reveal a relatively sophisticated larval nervous system in a well-studied gastropod, Ilyanassa obsoleta. The present study employed immunocytochemical and histofluorescent techniques combined with confocal microscopy to examine the development of cells containing monoamines (serotonin and catecholamine), neuropeptides (FMRFamide and leu-enkephalin related peptides), and a substance(s) reactive to antibodies raised against dopamine beta-hydroxylase. Neurons were first observed in the apical organ and posterior regions during the embryonic trochophore stage. During later embryonic development neurons appeared in peripheral regions such as the foot, velum, and mantle and in the developing ganglia destined to become the adult central nervous system. In subsequent free-swimming veliger stages the larval nervous system became increasingly elaborate and by late larval stages there existed approximately 26-28 apical cells, 80-100 neurons in the central ganglia, and 200-300 peripherally located neurons. During metamorphosis some populations of neurons in the apical organ and in the periphery disappeared, while others were incorporated into the juvenile nervous system. Comparisons of neural elements in other molluscan larvae reveal several similarities such as comparable arrangements of cells in the apical organ and patterns of peripheral cells. This investigation reveals the most extensive larval nervous system described in any mollusc to date and information from this study will be useful for future experimental studies determining the role of larval neurons and investigations of the cellular and molecular mechanisms governing neural development in this taxon.
Dickinson
Amanda J G
AJ
Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, Halifax, B3H 4H7 Nova Scotia, Canada. adickinson@wi.mit.edu
Croll
Roger P
RP
D000818:Animals; D005724:Ganglia / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology; D007814:Larva / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology; D009420:Nervous System / Q000033:anatomy & histology* / Q000737:chemistry; D017933:Peripheral Nervous System / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology; D012908:Snails / Q000033:anatomy & histology* / Q000737:chemistry / Q000502:physiology*