[Effects of tensile strain and loading time on the shape and cytoskeleton of the human periodontal ligament fibroblast measured by confocal laser scanning microscopy and immunity fluorescence technique].
The periodontal ligament fibroblast (PDLF) was cultivated artificially as the cell to be tested, and then it was loaded with mechanical stress-strain of different values and for different times. The cell and nucleus projected areas and shapes as well as the structure of cytoskeleton were tested by use of confocal laser scanning microscope and immunity fluorescence technique. Then the relationship among the stress-strain, the time, the shape and the structure of cytoskeleton of the PDLF was detected.


RESULTS
In the trial groups of 0, 8%, 12%, 16% strain values, the cell and nucleus projected areas were proportional to strain (stress) and time. The diameter, density and order of the structure of cytoskeleton increased in the strain and time dependent fashion. In the trial group of 20% strain values, the cell and nucleus projected areas decreased with the increase of time, and the structure of cytoskeleton became disorderly. It was demonstrated in this study that the shape and structure of cytoskeleton of PDLF underwent regular changes when the PDLF was loaded with the mechanical stress-strain.
Chen
Xinmin
X
West China College of Stomatology, Sichuan University, Chengdu 610041.
Hu
Jun
J
Ma
Jianfeng
J
Wu
Yan
Y
Gao
Ning
N
Hou
Rui
R
D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D011863:Radioimmunoassay; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology