title: Human antibodies from immunized donors are protective against anthrax toxin in vivo.
abstract: A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.
authors.affiliation: Alexion Antibody Technologies, Inc., 3985 Sorrento Valley Blvd., San Diego, California 92121, USA.
authors.forename: Martha A
authors.initials: MA
authors.lastname: Wild
authors.forename: Hong
authors.initials: H
authors.lastname: Xin
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Maruyama
authors.forename: Mary Jean
authors.initials: MJ
authors.lastname: Nolan
authors.forename: Peter M
authors.initials: PM
authors.lastname: Calveley
authors.forename: John D
authors.initials: JD
authors.lastname: Malone
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wallace
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Bowdish
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000276:immunology* / Q000633:toxicity; D001782:Blood Donors; D006801:Humans; D007109:Immunity / Q000502:physiology; D007114:Immunization / Q000379:methods; D007140:Immunoglobulin Fab Fragments / Q000097:blood* / Q000276:immunology*; D019151:Peptide Library; D051381:Rats
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; C030325:anthrax toxin