Microplate based biosensing with a computer screen aided technique.
Melanophores, dark pigment cells from the frog Xenopus laevis, have the ability to change light absorbance upon stimulation by different biological agents. Hormone exposure (e.g. melatonin or alpha-melanocyte stimulating hormone) has been used here as a reversible stimulus to test a new compact microplate reading platform. As an application, the detection of the asthma drug formoterol in blood plasma samples is demonstrated. The present system utilizes a computer screen as a (programmable) large area light source, and a standard web camera as recording media enabling even kinetic microplate reading with a versatile and broadly available platform, which suffices to evaluate numerous bioassays. Especially in the context of point of care testing or self testing applications these possibilities become advantageous compared with highly dedicated comparatively expensive commercial systems.
Filippini
Daniel
D
Division of Applied Physics, Institute of Physics and Measurement Technology, Linköping University, SE-581 83 Linköping, Sweden. danfi@ifm.liu.se
Andersson
Tony P M
TP
Svensson
Samuel P S
SP
Lundström
Ingemar
I
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016288:Computer Terminals; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004983:Ethanolamines / Q000032:analysis* / Q000494:pharmacology*; D000068759:Formoterol Fumarate; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008547:Melanophores / Q000166:cytology* / Q000187:drug effects*; D008550:Melatonin / Q000032:analysis / Q000494:pharmacology; D009862:Online Systems; D010784:Photomicrography / Q000295:instrumentation* / Q000379:methods; D014982:Xenopus laevis
D004983:Ethanolamines; D008550:Melatonin; D000068759:Formoterol Fumarate