[Changes and effects of group II metabotropic glutamate receptors in the cerebral cortex of rats after diffuse brain injuries coupled with hypopiesia secondary brain insults].
OBJECTIVE
To study the changes and effects of group II metabotropic glutamate receptors (mGluR) after diffuse brain injuries (DBI) coupled with hypopiesia secondary brain insults (SBI).


METHODS
Male SD rats were randomized into four groups: normal control, sham-operated, DBI alone and DBI coupled with SBI group. The SBI model was made on the basis of Marmarou's model. The mRNAs of the mGluRs were detected at 1, 3, 6, 12, 24, 48 and 72 hours after injuries by in-situ hybridization, and the positive neurons were counted.


RESULTS
Statistical analysis showed no significance between normal control group and sham-operated group, DBI group in mGluR 2, 3 mRNA (all P>0.05). The number of mGluR 2, 3 positive neurons decreased at 12 hours after injury and the peak occurred at 48 hours after injury in the injured cerebral cortex in DBI alone group (both P<0.05). However, in DBI with SBI group, there was a significant decrease of the number of mGluR2, 3 positive neurons at 6 hours after injury and the peak happened at 24 hours after injury (both P<0.05).


CONCLUSIONS
mGluR2, 3, factors with the function of protecting brain, might play an important role in the path physiology of DBI and SBI.
Fei
Zhou
Z
Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710033, Shanxi, China. Feizhou@fmma.edu.cn
Zhang
Xiang
X
Gong
Fang-he
FH
Bai
Hong-min
HM
Wang
Xi-ling
XL
Liang
Jing-wen
JW
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D007022:Hypotension / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics* / Q000502:physiology*; D013997:Time Factors
D018094:Receptors, Metabotropic Glutamate; C108822:metabotropic glutamate receptor 2