title: Phloroglucinol: novel synthesis and role of the magnesium cation on its binding with human serum albumin (HSA) using a biochromatographic approach based on Langmuir isotherms.
abstract: In this paper, a new and efficient method for synthesis of phloroglucinol with an overall yield of 60% was described. As well, the phloroglucinol association on an immobilized human serum albumin (HSA) column was analyzed in biochromatography by the determination of its Langmuir distribution isotherms. The role of the magnesium cation Mg2+ on the phloroglucinol-HSA binding process was as well analyzed. The results showed that in the Mg2+ concentration range (0.7-2 mM) (including its biological concentration range, i.e. 0.75-0.90 mM), increasing the Mg2+ concentration increased the fraction of free phloroglucinol (not linked with HSA) and thus its biological effect.
authors.affiliation: Equipe des Sciences Séparatives et Biopharmaceutiques (2SB), Laboratoires de Chimie Organique et Thérapeutique, Faculté de Médecine et de Pharmacie, Place Saint Jacques, F-25030 Besançon Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Ismaili
authors.forename: B
authors.initials: B
authors.lastname: Refouvelet
authors.forename: A
authors.initials: A
authors.lastname: Xicluna
authors.forename: J F
authors.initials: JF
authors.lastname: Robert
authors.forename: Y C
authors.initials: YC
authors.lastname: Guillaume
mesh_terms: D000465:Algorithms; D002845:Chromatography; D006801:Humans; D007202:Indicators and Reagents; D008274:Magnesium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D017711:Nonlinear Dynamics; D010696:Phloroglucinol / Q000138:chemical synthesis* / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D007202:Indicators and Reagents; D012709:Serum Albumin; D010696:Phloroglucinol; D008274:Magnesium