Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis.
Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.
Inglis
Timothy J J
TJ
Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia, Australia. tim.inglis@health.wa.gov.au
Aravena-Roman
Max
M
Ching
Simon
S
Croft
Kevin
K
Wuthiekanun
V
V
Mee
Brian J
BJ
D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000737:chemistry / Q000145:classification*; D016957:Burkholderia pseudomallei / Q000737:chemistry / Q000145:classification* / Q000472:pathogenicity; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D019814:Myristic Acid / Q000737:chemistry
D005227:Fatty Acids; D019814:Myristic Acid