title: [The influence of ammonium and nitrate of nutrient medium on ultrastructural organization and photosynthesis of callus cells in Glycine max L].
abstract: We studied the influence of exogenic ammonium on the functional activity and ultrastructural organization of cells of the mixotrophic soybean callus (Glycine max L.). Ammonium available in the nutrient medium increased the chlorophyll content, accelerating the rate of photosynthetic O2 evolution per unit of biomass. The presence of ammonium in the medium promoted formation of the protein-synthesizing system, which manifested itself as increased numbers of ribosomes, and thylakoids of chloroplasts, and higher electron density of the stroma in mitochondria and cytoplasm of mixotrophic cells. It has been concluded that the use of ammonium may lead to activation of protein synthesis, thus rising photosynthetic activity and favouring formation and development of membrane structures in chloroplasts.
authors.affiliation: Institute of Basic Biological Problems RAS, Pushchino, Moscow Region. ladygin@issp.serpukhov.su
authors.forename: V G
authors.initials: VG
authors.lastname: Ladygin
authors.forename: G A
authors.initials: GA
authors.lastname: Semenova
authors.forename: A P
authors.initials: AP
authors.lastname: Smolov
mesh_terms: D002478:Cells, Cultured; D002734:Chlorophyll / Q000032:analysis / Q000096:biosynthesis; D002736:Chloroplasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D003470:Culture Media; D008854:Microscopy, Electron; D009566:Nitrates / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012270:Ribosomes / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D020524:Thylakoids / Q000378:metabolism; D013997:Time Factors
chemical_list: D003470:Culture Media; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D002734:Chlorophyll; D010100:Oxygen