Cryopreservation of mango (Mangifera indica L.) embryogenic cultures.
Three techniques were compared for cryopreserving embryogenic masses (EMs) sampled from mango (Mangifera indica L.) cv. Zihua embryogenic cultures: (i) encapsulation/dehydration; (ii) pregrowth/dehydration; and (iii) vitrification. In all experiments, EMs were sampled from embryogenic cultures during their exponential growth phase and pretreated for 24 h on solid medium containing 0.5 M sucrose before freezing. No recovery was achieved after cryopreservation using the encapsulation/dehydration technique, whatever the moisture content (fresh weight basis) of EMs, which ranged from 78.3% without dehydration to 40.8% after 6 h dehydration. With the pregrowth plus dehydration technique, limited recovery (8.3%) was achieved after desiccation of EMs for 1 h, to 58.5% MC. Using the vitrification technique, recovery ranged from 94.3% after treatment of EMs with the PVS3 vitrification solution for 20 min (EM moisture content of 34.7%) to 10.9% after a 120 min treatment with the vitrification solution (EM moisture content of 26.0%).
Wu
Yong-Jie
YJ
The Key Laboratory of Gene Engineering of the Ministry of Education, Zhongshan University, Guangzhou, China.
Huang
Xue-Ling
XL
Xiao
Jie-Ning
JN
Li
Xiao-Ju
XJ
Zhou
Ming-De
MD
Engelmann
Florent
F
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D046508:Culture Techniques; D031022:Mangifera
D003451:Cryoprotective Agents