Liquid chromatographic determination of pyronaridine in human plasma and oral dosage form.
A new procedure for the determination of pyronaridine in plasma by reversed-phase high performance liquid chromatography (HPLC) with UV detection at 278 nm is described. The method involves liquid-liquid extraction of the drug with diethyl ether following basification of the deproteinized plasma with alkaline phosphate buffer. Chromatographic separation was achieved using a microbore C-18 column and a mobile phase consisting of 0.1% aqueous trifluoroacetic acid (TFA)-acetonitrile (75:25% (v/v)), pH 2.2, at a flow rate of 0.07 ml/min. Papaverine was used as internal standard. The response was linear between 50 and 1500 ng/ml. The limit of quantitation (LOQ) after plasma extraction was 50 ng/ml, the intra- and inter-day precision ranged from 2.5 to 13.8% (CV). The recovery of the drug from plasma and accuracy were >90%. Preliminary application of the method for monitoring pyronaridine in humans upon oral administration of the tablet demonstrated the principal usefulness of the assay for clinical trial studies. The method can also be used to analyze the compound in pharmaceutical formulations.
Babalola
Chinedum P
CP
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria. cbabalo@harthosp.org
Scriba
Gerhard K E
GK
Sowunmi
Akin
A
Alawode
Olaniyi A
OA
D000284:Administration, Oral; D000962:Antimalarials / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D004304:Dosage Forms; D006801:Humans; D009287:Naphthyridines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000962:Antimalarials; D004304:Dosage Forms; D009287:Naphthyridines; C027871:pyronaridine