Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP.
Mutations in the katG locus of catalase peroxidase in Mycobacterium tuberculosis (MTB) account for major isoniazid (INH) resistance. In the South China region, a collection of 906 respiratory specimens and 142 MTB isolates was used to evaluate the sensitivity and specificity of a PCR-RFLP method for the detection of INH resistance-associated mutations. Except for four catalase-negative MTB isolates, katG PCR for a 620-bp amplicon was successful for all purified MTB isolates. For respiratory specimens, diagnostic sensitivity and specificity of katG PCR was 85 and 100 %. Subsequent RFLP of the katG amplicons by MspI digestion identified that 51 % of INH-resistant MTB were associated with the Thr315 phenotype, and that codon 463 was a polymorphic site with no linkage to INH resistance. The Arg463 wild-type MTB isolates predominant in the Western world were replaced by isolates carrying Leu463 in the South China region. RFLP patterns of katG amplicons from respiratory specimens were identical to those of the corresponding MTB cultured colonies. This method has potential application for rapid diagnosis of INH resistance due to katG Ser315Thr mutation.
Leung
Eric Tung-Yiu
ET
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Kam
Kai-Man
KM
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Chiu
Agatha
A
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Ho
Pak-Leung
PL
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Seto
Wing-Hong
WH
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Yuen
Kwok-Yung
KY
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Yam
Wing-Cheong
WC
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China 2Tuberculosis Reference Laboratory and Public Health Laboratory, Department of Health, Hong Kong SAR, China 3,4Centre of Infection3 and HKU-Pasteur Research Centre4, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
D001426:Bacterial Proteins; D018893:Bronchoalveolar Lavage; D002374:Catalase; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013183:Sputum / Q000382:microbiology*; D014376:Tuberculosis / Q000382:microbiology*
D001426:Bacterial Proteins; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid