[Study of 8-OH-dG and its correlation with several cancer related gene in lung cancer tissues].
To investigate the relationship among 8-OH-dG and the development of human lung cancer and cancer related genes, an 8-OH-dG-specific monoclonal antibody and biotin-streptavidin immuno-staining were used to detect the 8-OH-dG in 150 cases of human lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The expressions of P53, C-MYC, K-RAS, BCL-2 and hTERT(human telomerase reverse transcriptase) were determined by immunohistochemistry and the relationship among the 8-OH-dG and these genes was analyzed. The 8-OH-dG were positive in 139 of 150 (92.7%) lung cancer specimens, and the percentage of adduct labeling cell in lung cancer specimens was (24.00 +/- 25.11)% (mean +/- SE). 21 of 120 (17.5%) adjacent lung tissues were adduct positive, and the percentage of adduct labeling cell was 2.42 +/- 5.98%. 4 of 40 (10.0%) benign lung lesions were adduct positive, and the percentage of adduct labeling cell was 0.80 +/- 1.30%, whereas 2 of 40 (5.0%) normal lung tissues were weak positive with 8-oh-dG, and the percentage of adduct labeling cell in this group was (0.34 +/- 1.01)%. The level of 8-OH-dG in lung cancer tissues was significantly higher than that of adjacent lung tissues, benign lung lesions and normal lungs (P < 0.01). The lung cancer patients were stratified by sex, age, cell types and smoking history, but these characteristics were not correlated with the level of 8-OH-dG. In the investigation of the relationship between the 8-OH-dG and five cancer related genes, higher 8-OH-dG levels were observed in lung cancer patients with over-expression of K-RAS and BCL-2 than those of negative expressed patients (P-value were 0.035 and 0.034 respectively), whereas the expression of P53, C-MYC and hTERT were not correlated with level of 8-OH-dG. 8-OH-dG was an important biomarker that may reflect the oxidative DNA damages of cells, and 8-OH-dG may affect K-RAS and BCL-2 genes in the carcinogenesis of lung cancer.
Lü
Jiachun
J
Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
Shi
Lüyuan
L
Wu
Zhongliang
Z
Liao
Yongde
Y
Zhou
Cheng
C
Li
Yinyan
Y
Bin
Xiaonong
X
Zeng
Bohang
B
Chen
Jaikun
J
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage; D004268:DNA-Binding Proteins; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D020598:Proto-Oncogene Proteins c-myb; D016159:Tumor Suppressor Protein p53; D000080242:8-Hydroxy-2'-Deoxyguanosine; D019098:Telomerase; D018631:ras Proteins; D003849:Deoxyguanosine