title: Planococcus rifietensis sp. nov, isolated from algal mat collected from a sulfurous spring in Campania (Italy).
abstract: The taxomony of strain M8, isolated from algal mat formed at the origin of a sulfurous spring in Rifieto (Savignano Irpino, Campania, Italy), was investigated in a polyphasic approach. The morphological, physiological and genetic characteristics were compared with of Planococcus and Planomicrobium species. The isolate grew optimally at pH 9.0, 1.8 M NaCl at 37 degrees C. The cells were Gram-positive cocci that form pairs, tetrads and aggregates of several cells. The isolate was aerobic/microaerophilic and accumulated glycine-betaine, as a major osmolyte, with minor components glutamate and an unknown compound. M8 was able to hydrolyse X-Glc (5-bromo-4-chloro-3-indoyl beta-d-glucopyranoside). The polar lipid profile consisted of phosphatidylglycerol and diphosphatidylglycerol as major components, and phosphocholine as a minor compound. MK8 was the only quinone found and the fatty acid composition was dominated by branched acids, mainly aiC15:0. The G+C content of DNA was 47.9% and its phylogenetic position was established by 16S rRNA gene sequencing as a member of the genus Planococcus. The DNA/DNA similarity of M8 to the type species Planococcus citreus was less than 55%. For this reason and for physiological and chemotaxonomic features, it is proposed to create a new species Planococcus rifietensis sp. nov.
authors.affiliation: Istituto di Chimica Biomolecolare, Comprensorio ex Olivetti, Pozzuoli, Na, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Romano
authors.forename: Assunta
authors.initials: A
authors.lastname: Giordano
authors.forename: Licia
authors.initials: L
authors.lastname: Lama
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
authors.forename: Agata
authors.initials: A
authors.lastname: Gambacorta
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D001483:Base Sequence; D016227:Benzoquinones / Q000032:analysis; D001622:Betaine / Q000378:metabolism; D002308:Cardiolipins / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D056890:Eukaryota / Q000254:growth & development / Q000382:microbiology; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006095:Gram-Positive Cocci / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007558:Italy; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000032:analysis; D010767:Phosphorylcholine / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
chemical_list: D016227:Benzoquinones; D002308:Cardiolipins; D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D010767:Phosphorylcholine; D001622:Betaine; C004532:quinone; D012965:Sodium Chloride