A quantitative in vitro assay method for detecting biological activity of endotoxin using prostaglandin E2 induction in rabbit peripheral blood.
The pyrogen test and the endotoxin test (the LAL test) have been playing crucial roles in detecting endotoxin in parenteral drugs. The current test methods, however, have disadvantages such as requiring a large number of animals or an inadequacy in evaluation of in vivo endotoxin activity. We attempted to establish a new assay method that can overcome the shortcomings of the current methods. We standardized the in vitro assay method by the use of prostaglandin E2 (PGE2) induction from peripheral blood of rabbits for detecting endotoxin activity. A linear dose-response regression was attained from approximately 0.15 to 5 endotoxin units/ml of Japanese national reference standard endotoxin by the in vitro assay. The assay showed a fine correlation with the pyrogen test but not with the LAL test, when endotoxins from various bacterial sources were tested. The in vitro assay was also shown to have the capability of detecting a synergistic effect of endotoxin and parenteral drugs. The in vitro PGE2 induction test using rabbit blood was, therefore, suggested to be the appropriate test method for guaranteeing the same level of safety of parenteral drugs as the pyrogen test does.
Ochiai
Masaki
M
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. masakio@nih.go.jp
Kataoka
Michiyo
M
Toyoizumi
Hiromi
H
Yamamoto
Akihiko
A
Kamachi
Kazunari
K
Horiuchi
Yoshinobu
Y
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards; D001773:Blood Cells / Q000187:drug effects*; D015232:Dinoprostone / Q000097:blood* / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity*; D066298:In Vitro Techniques; D011817:Rabbits
D004731:Endotoxins; D015232:Dinoprostone