csp-like genes of Lactobacillus delbrueckii ssp. bulgaricus and their response to cold shock.
The two csp-like genes from the lactic acid bacterium Lactobacillus delbrueckii ssp. bulgaricus were characterized and designated cspA and cspB. The gene cspA has been identified using a polymerase chain reaction (PCR)-based approach with degenerated primers and further characterized using an inverse PCR strategy. cspA encodes a protein of 65 amino acid residues which displays between 81 and 77% identity with proteins CspL and CspP of Lactobacillus plantarum. cspB has been identified as a cspA ortholog using the partial sequence of the L. bulgaricus ATCC11842. cspB encodes a protein of 69 amino acids which has 42% identity with CspA. Northern blot analyses showed that cspA is transcribed as a single gene and that its transcription increased after a temperature downshift from 42 to 25 degrees C. In contrast, cspB is part of an operon transcribed at constant level irrespective of the temperature. These results indicate that cspA encodes the only Csp-like protein of L. bulgaricus induced by a downshift of temperature.
Serror
Pascale
P
Unité Recherche Laitière et Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. serror@jouy.inra.fr
Dervyn
Rozenn
R
Ehrlich
S Dusko
SD
Maguin
Emmanuelle
E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; C105798:CspL protein, Lactobacillus plantarum; C105800:CspP protein, Lactobacillus plantarum; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; C062260:cold shock protein CS7.4, Bacteria; C077188:cold-shock protein CspB, Bacteria