Pyrrolidino-DNA.
We synthesized pyrrolidino-C-nucleosides, incorporated them into oligodeoxynucleotides and investigated their pairing properties. The thermal duplex and triplex stabilities were measured. While triplex formation is destabilized in the case of pyrrolidino-pseudo-U and -T, pyrrolidino-pseudo-iso-C leads to an increase of the Tm value for third strand dissociation. Duplexes are destabilized with all pyrrolidino-C-nucleosides.
Häberli
Adrian
A
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
Mayer
Alain
A
Leumann
Christian J
CJ
D003596:Cytosine / Q000031:analogs & derivatives; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D007202:Indicators and Reagents; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011759:Pyrrolidines; D013816:Thermodynamics; D014498:Uracil / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011759:Pyrrolidines; D014498:Uracil; C008378:6-methyluracil; D003596:Cytosine; D004247:DNA