RAPD analyses of recombination processes in the entomopathogenic fungus Beauveria bassiana.
To understand the nature of recombination processes in Beauveria bassiana, double-auxotrophic complementary mutant strains were used to produce six heterokaryons by three different methods. Conidia from these heterokaryons were plated on selective media and stable haploid (but not diploid) recombinants were isolated. Single colony recombinants were recovered with both parental and non-parental random amplified polymorphic DNA (RAPD) profiles. These results suggest that a range of different recombination mechanisms may be occurring in B. bassiana.
Dalzoto
Patricia R
PR
Universidade Federal do Paraná, Departamento de Genética, C. Postal 19071, Curitiba PR, CEP 81531-990, Brazil. pdalzoto@terra.com.br
Glienke-Blanco
Chirlei
C
Kava-Cordeiro
Vanessa
V
Araújo
Welington Luiz
WL
Azevedo
João Lúcio
JL
D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D006238:Haploidy; D006999:Hypocreales / Q000235:genetics* / Q000472:pathogenicity; D007313:Insecta / Q000382:microbiology; D009154:Mutation; D010572:Pest Control, Biological; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic
D004271:DNA, Fungal