A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli.
A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions.
Chen
Chin Yi
CY
Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. cchen@errc.ars.usda.gov
Nace
Gary W
GW
Irwin
Peter L
PL
D000362:Agar / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000254:growth & development*; D008089:Listeria monocytogenes / Q000254:growth & development*
D003470:Culture Media; D000362:Agar