[Isolation of myoinositol from solutions of enzymatic biotransformation].
The transferase reaction between phospholipids and inositol catalyzed by phospholipase D on phase interface in water-organic solvent systems was studied. Optimal conditions for phosphatidylinositol synthesis in water-organic solvent heterogeneous system were determined. The rapid separation of the hydrophobic components, phospholipids, from water-soluble products, alcohols, was observed in the systems with organic solvents. Displacement of myo-inositol from phosphatidylinositol by methanol, alcohol substrate, added to the reaction medium was shown in hexane-water system. Myo-inositol was isolated from the mixture of its isomers by two-stage transferase reaction catalyzed by phospholipase D.
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Mirzo Ulugbek Uzbekistan National University, Biological and Soil Department, Tashkent, 700174 Uzbekistan.
Rakhimov
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D004798:Enzymes / Q000378:metabolism*; D005285:Fermentation; D007294:Inositol / Q000302:isolation & purification*; D012996:Solutions
D004798:Enzymes; D012996:Solutions; D007294:Inositol