title: [Detection and analysis of SARS coronavirus-specific antibodies in sera from non-SARS children].
abstract: OBJECTIVE
  To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children.
  
  
  METHODS
  Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1,060 non-SARS children in Guangzhou.
  
  
  RESULTS
  All the serum samples from the 1,060 non-SARS children were negative for both IgG and IgM antibodies against SARS coronavirus as determined by indirect immunofluorescent assay, with only two serum samples showing weak positivity for SARS coronavirus-specific antibodies identified by double-antigen sandwich ELISA.
  
  
  CONCLUSIONS
  No SARS coronavirus-specific antibody are present in the sera of non-SARS children.
authors.affiliation: Department of Clinical Laboratory, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. linhaili@hotmail.com
authors.forename: Lin-hai
authors.initials: LH
authors.lastname: Li
authors.forename: Yu-ling
authors.initials: YL
authors.lastname: Shi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: De-xing
authors.initials: DX
authors.lastname: Xu
authors.forename: Gen-ping
authors.initials: GP
authors.lastname: Wan
authors.forename: Xiao-qiong
authors.initials: XQ
authors.lastname: Gu
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Zhang
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Ma
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral