title: Origin of apparent negative cooperativity of F(1)-ATPase.
abstract: In order to get insight into the origin of apparent negative cooperativity observed for F(1)-ATPase, we compared ATPase activity and ATPMg binding of mutant subcomplexes of thermophilic F(1)-ATPase, alpha((W463F)3)beta((Y341W)3)gamma and alpha((K175A/T176A/W463F)3)beta((Y341W)3)gamma. For alpha((W463F)3)beta((Y341W)3)gamma, apparent K(m)'s of ATPase kinetics (4.0 and 233 microM) did not agree with apparent K(m)'s deduced from fluorescence quenching of the introduced tryptophan residue (on the order of nM, 0.016 and 13 microM). On the other hand, in case of alpha((K175A/T176A/W463F)3)beta((Y341W)3)gamma, which lacks noncatalytic nucleotide binding sites, the apparent K(m) of ATPase activity (10 microM) roughly agreed with the highest K(m) of fluorescence measurements (27 microM). The results indicate that in case of alpha((W463F)3)beta((Y341W)3)gamma, the activating effect of ATP binding to noncatalytic sites dominates overall ATPase kinetics and the highest apparent K(m) of ATPase activity does not represent the ATP binding to a catalytic site. In case of alpha((K175A/T176A/W463F)3)beta((Y341W)3)gamma, the K(m) of ATPase activity reflects the ATP binding to a catalytic site due to the lack of noncatalytic sites. The Eadie-Hofstee plot of ATPase reaction by alpha((K175A/T176A/W463F)3)beta((Y341W)3)gamma was rather linear compared with that of alpha((W463F)3)beta((Y341W)3)gamma, if not perfectly straight, indicating that the apparent negative cooperativity observed for wild-type F(1)-ATPase is due to the ATP binding to catalytic sites and noncatalytic sites. Thus, the frequently observed K(m)'s of 100-300 microM and 1-30 microM range for wild-type F(1)-ATPase correspond to ATP binding to a noncatalytic site and catalytic site, respectively.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, 226-8503 Yokohama, Japan.
authors.forename: Sakurako
authors.initials: S
authors.lastname: Ono
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Jun
authors.initials: J
authors.lastname: Hirao
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsui
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Noji
authors.forename: Masasuke
authors.initials: M
authors.lastname: Yoshida
authors.forename: Eiro
authors.initials: E
authors.lastname: Muneyuki
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001665:Binding Sites; D002384:Catalysis; D004789:Enzyme Activation; D008274:Magnesium / Q000737:chemistry*; D008956:Models, Chemical; D009097:Multienzyme Complexes / Q000737:chemistry; D011485:Protein Binding; D021122:Protein Subunits; D006180:Proton-Translocating ATPases / Q000737:chemistry*
chemical_list: D009097:Multienzyme Complexes; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D008274:Magnesium