title: Teratogenic effect of broad-band electromagnetic field on neonatal mice (Mus musculus).
abstract: Pregnant mice (Mus musculus), strain Swiss Webster, were exposed to a continuous electromagnetic field (12.8V/m) beginning in the third week of pregnancy. Histological and hematological analysis showed gender specific responses in 21 day-old mice after in-utero and post-natal continuous exposure. Automated lymphocyte percentage and total white blood cell counts were significantly elevated in exposed 21 day-old female mice compared to control mice. Lymphoma-like cells were seen in higher numbers in exposed 21 day-old male mice. Megaloblastic changes, such as hypersegmented neutrophils, were observed in exposed mice. The blood from control neonatal mice was more viscous than that of exposed mice, enough to interfere with making a blood smear. The adult female mice showed no significant differences in the above hematologic parameters between exposed and control groups. Histological study showed the following pathological changes in the adrenal cortex: degeneration/necrosis in the zona glomerulosa; hypertrophy in zona reticularis; degeneration/necrosis, intracytoplasmic inclusions and inflammation in the zona fasciculata/reticularis, more prominent in exposed female neonates; and lipidosis in the zona fasciculata. In the adrenal medulla: atrophy was more common in exposed female neonates; and intracytoplasmic inclusions and vacuolation were more common in exposed male neonates. Cystic proliferations were found in the cortical area of the thymus. In the medulla of the thymus, there was vacuolation, inflammation, or eosinophilic intracytoplasmic inclusions in exposed adults. Behavioral differences occurred in both neonates and adult females. Control neonates were able to manipulate through a maze more quickly than exposed neonates; and control adult females displayed more thorough grooming behavior than exposed mothers, and maintained more distance between the nest and dropping location than did the exposed group.
authors.affiliation: Department of Environmental Science, Marist College, Poughkeepsie, New York 12601, USA. Zofia.Gagnon@Marist.edu
authors.forename: Z E
authors.initials: ZE
authors.lastname: Gagnon
authors.forename: C
authors.initials: C
authors.lastname: Newkirk
authors.forename: J A
authors.initials: JA
authors.lastname: Conetta
authors.forename: M A
authors.initials: MA
authors.lastname: Sama
authors.forename: S
authors.initials: S
authors.lastname: Sisselman
mesh_terms: D000311:Adrenal Glands / Q000473:pathology*; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D004249:DNA Damage; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005260:Female; D018655:Lymphocyte Count; D008223:Lymphoma / Q000209:etiology*; D008297:Male; D051379:Mice; D013950:Thymus Gland / Q000473:pathology