Dendritic stability in the adult olfactory bulb.
In many regions of the adult mammalian brain, pronounced changes in synaptic input caused by lesions or severe sensory deprivation induce marked sprouting or retraction of neuronal dendrites. In the adult olfactory bulb, adult neurogenesis produces less pronounced, but continuously ongoing synapse turnover. To test the structural stability of adult dendrites in this context, we used two-photon microscopy to image dendrites of mitral and tufted (M/T) cells over prolonged periods in adult mice. Although pharmacologically increased activity could elicit morphological changes, under natural conditions such as ongoing neurogenesis, an odor-enriched environment or olfactory-based learning, M/T cell dendrites remained highly stable. Thus, in a context of ongoing adult synaptogenesis, dendritic stability could serve as a structural scaffold to maintain the organization of local circuits.
Mizrahi
Adi
A
Howard Hughes Medical Institute and the Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA. mizrahi@neuro.duke.edu
Katz
Lawrence C
LC
D000098:Acetophenones / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001522:Behavior, Animal; D001640:Bicuculline / Q000494:pharmacology; D003214:Conditioning, Classical; D003712:Dendrites / Q000502:physiology*; D004193:Discrimination Learning / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D018756:GABA Antagonists / Q000494:pharmacology; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D009415:Nerve Net / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology / Q000821:virology; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D017785:Photons; D011336:Probability; D013268:Stimulation, Chemical; D013569:Synapses / Q000502:physiology*; D013997:Time Factors; D014162:Transfection / Q000662:veterinary
D000098:Acetophenones; D000447:Aldehydes; D001426:Bacterial Proteins; D018756:GABA Antagonists; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; C018475:butyraldehyde; C038699:acetophenone; D001640:Bicuculline