title: Acetylcholinesterase level and molecular isoforms are altered in brain of Reelin Orleans mutant mice.
abstract: In this study we examined changes in acetylcholinesterase (AChE) pattern in the brain of adult Reelin Orleans (RelnOrl) homozygous mutant mice. The AChE histochemistry firstly revealed an abnormal distribution of AChE-positive cells in several areas of the reeler brain, including cortices; the strongest labelling was observed in cerebellum and hippocampus when compared with controls. Biochemical determinations demonstrated an increase of 80-90% in AChE specific activity from cerebellar and hippocampal extracts. We also report that the AChE tetrameric form (G4) was selectively increased in the RelnOrl brain. The relationship between AChE and Reelin and suggested morphogenetic functions are also discussed.
authors.affiliation: Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, San Juan de Alicante, Spain.
authors.forename: M Salud
authors.initials: MS
authors.lastname: García-Ayllón
authors.forename: Dolores
authors.initials: D
authors.lastname: Seguí
authors.forename: Mercedes
authors.initials: M
authors.lastname: Perales
authors.forename: Edith
authors.initials: E
authors.lastname: López-Hurtado
authors.forename: Jorge J
authors.initials: JJ
authors.lastname: Prieto
authors.forename: Javier
authors.initials: J
authors.lastname: Sáez-Valero
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D002531:Cerebellum / Q000201:enzymology / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000201:enzymology / Q000473:pathology; D006720:Homozygote; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008818:Mice, Neurologic Mutants; D019581:Neuropil / Q000201:enzymology / Q000473:pathology; D011689:Purkinje Cells / Q000201:enzymology / Q000473:pathology; D000091042:Reelin Protein
chemical_list: D007527:Isoenzymes; D000091042:Reelin Protein; D000110:Acetylcholinesterase; C000717128:Reln protein, mouse