Factors in intestinal lymph after shock increase neutrophil adhesion molecule expression and pulmonary leukosequestration.
BACKGROUND
Because the ischemic gut may produce factors that initiate systemic inflammation, we tested the hypothesis that factors released from the gut into the mesenteric lymphatics increase neutrophil (PMN) adhesion molecule expression after trauma and shock.


METHODS
At 1 and 4 hours after hemorrhagic shock (30 mm Hg x 90 minutes) plus trauma (laparotomy) (T/HS) or sham-shock (T/SS), with or without mesenteric lymph duct ligation, PMN CD11b and CD18 expression was assessed in male rats. In additional rats, mesenteric lymph samples were tested for their ability to increase PMN CD11b expression in vitro. Lastly, at 4 hours after T/SS or T/HS with or without lymph duct ligation, pulmonary PMN sequestration was measured.


RESULTS
Compared with T/SS rats, T/HS was associated with up-regulation of PMN CD11b and CD18 expression, which was largely prevented by ligation of the mesenteric lymph duct (p < 0.01). Lymph duct ligation also prevented T/HS-induced pulmonary leukocyte sequestration (p < 0.01). In addition, mesenteric lymph from rats subjected to T/HS but not T/SS increased CD11b expression (p < 0.01).


CONCLUSIONS
Factors produced or released by the postischemic intestine and carried in the mesenteric lymph appear responsible for PMN activation and pulmonary PMN sequestration after an episode of T/HS.
Caruso
Joseph M
JM
Department of Surgery, New Jersey Medical School, Newark, New Jersey 07101-1709, USA. edeitch@umdnj.edu
Feketeova
Eleonora
E
Dayal
Sara D
SD
Hauser
Carl J
CJ
Deitch
Edwin A
EA
D000704:Analysis of Variance; D000818:Animals; D001998:Bronchopulmonary Sequestration / Q000276:immunology*; D039481:CD11b Antigen / Q000096:biosynthesis*; D018821:CD18 Antigens / Q000096:biosynthesis*; D007813:Laparotomy / Q000009:adverse effects; D008026:Ligation; D008196:Lymph / Q000502:physiology; D008297:Male; D008643:Mesentery; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000276:immunology*; D015854:Up-Regulation
D039481:CD11b Antigen; D018821:CD18 Antigens