[Effect of human telomerase reverse transcriptase mRNA introduction on the growth of human umbilicus vein endothelial cell].
OBJECTIVE
To elongate the proliferation life-span of human umbilicus vein endothelial cell (HUVEC).


METHODS
We synthesized the human telomerase reverse transcriptase mRNA (hTERT mRNA) by in vitro transcription, then transferred the hTERT mRNA into HUVEC in quicent stage by lipofect introduction.


RESULTS
Telomerase expressed transiently in HUVEC, and the cell life-span was elongated for 7 population doublings.


CONCLUSIONS
Telomerase can be reconstructed controllably and transiently in HUVEC by hTERT mRNA introduction, this method has the potential to be used to elongate the lifespan of cells cultured in vitro.
Qu
Yi
Y
Laboratory of Medical Molecular Biology, School of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan, P. R. China 610041. quyi712002@yahoo.com.cn
Wang
Zheng-rong
ZR
Huang
Xiang
X
D002455:Cell Division; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D019098:Telomerase