title: Prolonged cardiac xenograft survival in guinea pig-to-rat model by a highly active cobra venom factor.
abstract: A highly active cobra venom factor (CVF) was isolated from the venom of Naja kaouthia by sequential column chromatography. It displays strong anticomplementary activity, and has 1515 U of anticomplementary activity per mg protein. A single dose of 0.1 mg/kg CVF given i.v. to rats completely abrogated complement activity for nearly 5 days. Given 0.02 mg/kg of CVF, the complement activity of rats was reduced by more than 96.5% in 6 h. In guinea pig-to-rat heart transplant model, rats treated with a single dose of 0.05 mg/kg CVF had significantly prolonged xenograft survival (56.12+/-6.27 h in CVF-treated rats vs. 0.19+/-0.07 h in control rats, P<0.001).
authors.affiliation: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. sunqy@hotmail.com
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Sun
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Wang
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Xiong
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology; D003169:Complement Inactivator Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000737:chemistry / Q000494:pharmacology*; D006084:Graft Rejection / Q000473:pathology; D006085:Graft Survival / Q000187:drug effects*; D006168:Guinea Pigs; D016027:Heart Transplantation / Q000502:physiology*; D008297:Male; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous / Q000502:physiology*
chemical_list: D001685:Biological Factors; D003169:Complement Inactivator Proteins; D004546:Elapid Venoms