Modification of redox sites of N-methyl-D-aspartate receptors affects anoxia-induced changes in the bioelectrical activity of rat brain olfactory cortex slices.
Living slices of Wistar-Kyoto rat brain olfactory cortex were used to study the effects of the thiol-oxidizing agent 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), which inhibits NMDA receptor activity, on changes in the generation of evoked focal potentials (NMDA and non-NMDA EPSP) in response to long-term and short-term anoxia, which induces functional damage and facilitates increases in the resistance of neurons to severe hypoxia respectively. These studies showed that DTNB (200 microM) efficiently prevented the suppression of focal EPSP generation due to long-term anoxia in most slices. In addition, DTNB partially reversed the protective effect of preconditioning with short-term anoxia on the impairment of focal EPSP generation induced by long-term anoxia. This affected the NMDA component of the EPSP to a greater extent than the non-NMDA component. The possible role of changes in the state of modulatory redox sites of NMDA receptors in the mechanisms of functional damage and increases in neuron resistance due to hypoxia is discussed.
Samoilov
M O
MO
Laboratory for the Regulation of Brain Neuron Function, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034 St. Petersburg, Russia.
Mokrushin
A A
AA
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D019194:Ischemic Preconditioning; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009833:Olfactory Pathways / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D051381:Rats; D011921:Rats, Inbred WKY; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000378:metabolism*
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D004228:Dithionitrobenzoic Acid