Isolation and anti-oomycete activity of nyasol from Anemarrhena asphodeloides rhizomes.
The methanol extract of Anemarrhena asphodeloides rhizomes exhibited strong antifungal activity against the plant pathogenic fungi Magnaphothe grisea, Rhizoctonia solani, and the plant pathogenic oomycete Phytophthora capsici. The antifungal substance isolated from the rhizomes of A. asphodeloides was identified to be nyasol, (Z)-1,3-bis(4-hydroxyphenyl)-1,4-pentadiene by NMR and mass spectral analysis. Nyasol effectively inhibited the mycelial growth of Colletotrichum orbiculare, P. capsici, Pythium ultimum, R. solani, and Cladosporium cucumerinum in a range of 1-50 mug/ml, but did not affect the growth of bacteria and yeast. In a greenhouse test, treatment with the antifungal compound nyasol was significantly effective in suppressing the Phytophthora blight on pepper plants.
Park
Hee Jin
HJ
Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, Seoul 136-701, South Korea.
Lee
Jung Yeop
JY
Moon
Surk Sik
SS
Hwang
Byung Kook
BK
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D031403:Anemarrhena / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002212:Capsicum / Q000382:microbiology; D017705:Lignans; D008826:Microbial Sensitivity Tests; D009868:Oomycetes / Q000187:drug effects* / Q000254:growth & development; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010838:Phytophthora / Q000187:drug effects / Q000254:growth & development; D027343:Rhizome / Q000737:chemistry
D000935:Antifungal Agents; D017705:Lignans; D010636:Phenols; C028175:metalaxyl; C109393:nyasol; D000409:Alanine