The Ro52/SS-A autoantigen has elevated expression at the brain microvasculature.
An expression cloning technique was used to identify proteins selectively expressed at the blood-brain barrier that may mediate interactions between the brain microvasculature and IgG molecules. Ro52/SS-A is an IgG-binding protein and is an autoantigen implicated in Sjogren's syndrome and systemic lupus erythematosus. The present studies describe the expression cloning of this gene product from a bovine brain microvascular cDNA library. The Ro52 protein interacted with IgG molecules independent of specificity suggesting a potential role as a general antibody receptor. Northern blotting shows the Ro52/SS-A transcript is enriched in the microvascular compartment of brain. The selective expression of Ro52/SS-A in brain at the microvasculature may play a role in brain vascular involvement in autoimmune diseases.
Shusta
Eric V
EV
Department of Chemical and Biological Engineering, University of Wisconsin, Madison, WI 53706, USA.
Li
Jian Yi
JY
Boado
Ruben J
RJ
Pardridge
William M
WM
D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D001812:Blood-Brain Barrier / Q000378:metabolism*; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D019556:COS Cells / Q000378:metabolism; D002196:Capillaries / Q000378:metabolism*; D002417:Cattle; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000099207:SS-A Antigen
D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human