In vivo lipoprotein binding assay of the insect exchangeable apolipoprotein, apolipophorin-III.
An original method for the study of the lipid binding properties of exchangeable apolipoproteins is reported. Binding of Locusta migratoria apolipophorin-III to Manduca sexta low-density lipophorin (LDLp) and high-density lipophorin (HDLp) was studied in vivo. This assay could be used useful to investigate the effect of mutations in the lipid binding properties of exchangeable apolipoproteins under physiological conditions.
Chetty
Palaniappan S
PS
147 Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. jose@biochem.okstate.edu
Arrese
Estela L
EL
Soulages
Jose L
JL
D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006110:Grasshoppers; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D018402:Manduca; D014461:Ultracentrifugation
D001053:Apolipoproteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; C042470:apolipophorin III; C032830:lipophorin