Trypanosoma rangeli uptakes the main lipoprotein from the hemolymph of its invertebrate host.
During its life cycle Trypanosoma rangeli crosses the hemolymph of its invertebrate host. In the present study, we demonstrate for the first time the uptake of lipophorin (Lp), the main lipid-transporting particle of insect hemolymph. We observed that living T. rangeli parasites uptake lipids from both 32P- and 3H-, or 125I-labeled Lp. However, the parasites do not uptake any other hemolymphatic protein such as 32P-labeled vitellogenin. The presence of a specific receptor to Lp in the parasite surface is suggested based on experiments using 125I-Lp. We also investigated the intracellular fate of lipids using Texas Red-labeled phosphatidylethanolamine-Lp. Parasites were observed under confocal microscope and displayed fluorescent-labeled lipids close to the flagellar pocket and in vesicles at the posterior region. In conclusion, this study raises a novel set of molecular events which takes place during vector-parasite interaction.
Folly
Evelize
E
Departamento de Bioquímica Médica, ICB, CCS, Bloco H, Universidade Federal do Rio de Janeiro, UFRJ, Cidade Universitária, Rio de Janeiro CEP 21941-590, RJ, Brazil.
Cunha e Silva
Narcisa L
NL
Lopes
Angela H C S
AH
Silva-Neto
Mário A C
MA
Atella
Georgia C
GC
D000818:Animals; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism*; D004705:Endocytosis; D006458:Hemolymph / Q000378:metabolism; D006790:Host-Parasite Interactions; D007303:Insect Vectors / Q000378:metabolism*; D007700:Kinetics; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D012239:Rhodnius / Q000378:metabolism* / Q000469:parasitology; D014345:Trypanosoma / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
D002352:Carrier Proteins; D008074:Lipoproteins; C032830:lipophorin