Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines.
We examined the effects of inactivation of the RB gene on chemosensitivity of human osteosarcoma cell lines, using the MTT assay and calculating the inhibition index. Although the human osteosarcoma cell lines HOS and MG63 have a wild-type RB gene, SaOS-2 and OSrb (established from retinoblastoma patient) have no active RB gene. We used these 4 cell lines in growth inhibition assays for doxorubicin, cisplatin and methotrexate, and assessed the chemosensitivity. In the growth inhibition assay for methotrexate, cell lines lacking an active RB gene were more resistant than cell lines with an active RB gene.
Iida
Koji
K
Department of Orthopedic Surgery, Mie University School of Medicine, Edobashi 2-174, Tsu City, Mie Prefecture 514-8507, Japan. n73cfq1p@ztv.ne.jp
Nobori
Tsutomu
T
Matsumine
Akihiko
A
Isaka
Akihiko
A
Seto
Masashi
M
Shiraishi
Taizou
T
Uchida
Atsumasa
A
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D015152:Blotting, Northern; D015153:Blotting, Western; D002455:Cell Division; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D004396:Coloring Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology; D012516:Osteosarcoma / Q000378:metabolism*; D018807:Polymorphism, Single-Stranded Conformational; D016160:Retinoblastoma Protein / Q000096:biosynthesis* / Q000235:genetics; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D004396:Coloring Agents; D016160:Retinoblastoma Protein; D013778:Tetrazolium Salts; D013844:Thiazoles; D004317:Doxorubicin; C022616:thiazolyl blue; D002945:Cisplatin; D008727:Methotrexate