[Production of a new monoclonal anti-AB reagent].
Techniques of monoclonal antibodies production have well progressed in the developed countries. But, they are still absent in the third world. In this report, the murine hybridoma technology was performed to produce anti-ABH monoclonal antibodies. The anti-AB specificity of several hybridomas was determined by agglutination techniques using phenotyped red cells panels. An anti-AB monoclonal antibody was selected and compared to other reagents for its use as a blood grouping reagent. Its characteristics were in accordance with the international standards. Hybridoma establishment to produce a good monoclonal reagent is henceforth possible in Morocco.
Habti
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N
Laboratoire de génie génétique et cellulaire, service d'hématologie, faculté de médecine et de pharmacie, 19, rue Tarik-Ibnou-Ziad, Casablanca, Maroc. habtino@hotmail.com
Tissent
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A
Benchemsi
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N
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001803:Blood Transfusion / Q000592:standards; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D009018:Morocco; D015203:Reproducibility of Results
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal