Application of Amido black staining to enumerating bacteria grown on membrane filters.
A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method.
Inatomi
Ken-ichi
K
Advanced Technology R&D Center, Mitsubishi Electric Corp., 811, Tsukaguchi, Amagasaki, Hyogo 661, Japan. Inatomi.Kenichi@wrc.melco.co.jp
D000580:Amido Black / Q000378:metabolism*; D001412:Bacillus subtilis / Q000254:growth & development* / Q000302:isolation & purification; D004396:Coloring Agents / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification; D005374:Filtration; D008567:Membranes, Artificial; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000302:isolation & purification; D013194:Staining and Labeling; D014871:Water Microbiology
D004396:Coloring Agents; D008567:Membranes, Artificial; D000580:Amido Black