Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting.
Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.
McAuliffe
Laura
L
Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Surrey, KT15 3NB, United Kingdom. l.mcauliffe@vla.defra.gsi.gov.uk
Ellis
Richard J
RJ
Ayling
Roger D
RD
Nicholas
Robin A J
RA
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S