title: Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry.
abstract: In recent years, consumption of herbal supplements as an alternative to pharmaceutical drug therapy has increased. For example, with the health claims labeling which describes the link between soy-protein and a reduced risk of coronary heart disease (CHD), the consumption of soy and soy-derived phytoestrogens has increased dramatically. That being said, the oral bioavailability of only a few soy phytoestrogens such as Daidzein and Genestein have been previously estimated. In this paper, we present the calculated percent of rat oral bioavailability of five soy-derived phytoestrogens (Genistein, Daidzein, Biochanin A, Coumestrol, and Zearalenone) in male Sprague-Dawley rats. The plasma quantitation required for the bioavailability calculation is performed by using a rapid on-line plasma extraction procedure for the quantitative analysis. To further speed up the analysis the rats were dosed using the 'n-in-one' (cassette) protocol. The rapid on-line extraction/quantitation methodology coupled to the cassette dosing analysis of phytoestrogens is the key point of this paper. The limit of quantitation (LOQ) for each compound was 1-1000 ng/ml with each plasma sample analysis taking less than 2 min. In general the percent oral bioavailability was determined to be between 11 and 28%.
authors.affiliation: Wyeth Research, Discovery Analytical Chemistry, Chemical and Screening Sciences, 500 Arcola Rd. RS-2402D, Collegeville, PA 19426, USA. mallisl@wyeth.com
authors.forename: Larry M
authors.initials: LM
authors.lastname: Mallis
authors.forename: Ani B
authors.initials: AB
authors.lastname: Sarkahian
authors.forename: Heather A
authors.initials: HA
authors.lastname: Harris
authors.forename: Mei-Yi
authors.initials: MY
authors.lastname: Zhang
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: McConnell
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001331:Automation; D001682:Biological Availability; D007529:Isoflavones / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D048789:Phytoestrogens; D028321:Plant Preparations / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations