Development of a mariner-based transposon for use in Sorangium cellulosum.
In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.
Julien
Bryan
B
Kosan Biosciences, Inc., Hayward, California 94545, USA. julien@kosan.com
Fehd
Ruby
R
D001426:Bacterial Proteins / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009231:Myxococcales / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics; D019895:Transposases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D003577:Cytochrome P-450 Enzyme System; C403885:EpoK protein, Polyangium cellulosum; D010088:Oxidoreductases; D019895:Transposases