title: Novel method for the covalent immobilization of oligonucleotides via Diels-Alder bioconjugation.
abstract: The synthesis of cyclohexadiene and maleimide derivatives and their use for the functionalization of oligonucleotides and the coating of glass surfaces is reported. A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented.
authors.affiliation: Proligo LLC, Boulder, Colorado 80301, USA.
authors.forename: Hallie A
authors.initials: HA
authors.lastname: Latham-Timmons
authors.forename: Andreas
authors.initials: A
authors.lastname: Wolter
authors.forename: J Shawn
authors.initials: JS
authors.lastname: Roach
authors.forename: Rubina
authors.initials: R
authors.lastname: Giare
authors.forename: Michael
authors.initials: M
authors.lastname: Leuck
mesh_terms: D002625:Chemistry, Organic / Q000379:methods; D005898:Glass; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides