A mathematical model for analysis of the cell cycle in cell lines derived from human tumors.
The growth of human cancers is characterised by long and variable cell cycle times that are controlled by stochastic events prior to DNA replication and cell division. Treatment with radiotherapy or chemotherapy induces a complex chain of events involving reversible cell cycle arrest and cell death. In this paper we have developed a mathematical model that has the potential to describe the growth of human tumour cells and their responses to therapy. We have used the model to predict the response of cells to mitotic arrest, and have compared the results to experimental data using a human melanoma cell line exposed to the anticancer drug paclitaxel. Cells were analysed for DNA content at multiple time points by flow cytometry. An excellent correspondence was obtained between predicted and experimental data. We discuss possible extensions to the model to describe the behaviour of cell populations in vivo.
Basse
Britta
B
Biomathematics Research Centre, University of Canterbury, Private Bag, 4800, Christchurch, New Zealand. britta.basse@canterbury.ac.nz
Baguley
Bruce C
BC
Marshall
Elaine S
ES
Joseph
Wayne R
WR
van Brunt
Bruce
B
Wake
Graeme
G
Wall
David J N
DJ
D000465:Algorithms; D000970:Antineoplastic Agents / Q000494:pharmacology; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D003198:Computer Simulation; D004273:DNA, Neoplasm / Q000032:analysis; D005057:Eukaryotic Cells / Q000737:chemistry / Q000187:drug effects; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects / Q000502:physiology; D016195:G2 Phase / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D008954:Models, Biological; D017239:Paclitaxel / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects / Q000502:physiology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D017239:Paclitaxel