[The effect of endotoxin on the expression of tissue factor in the CCl4-treated rat liver].
OBJECTIVE
Endotoxemia is known to cause hepatic microcirculation disturbance and hepatic injury through blood coagulation and thrombosis in the cirrhotic patients. This study was to investigate the effect of endotoxin on the expression of tissue factor in rat liver with persistent injury.


METHODS
Male Sprague-Dawley rats were used for this experiment. Persistent hepatic injury was induced by injecting 0.1 mL of CCl4 and 0.1 mL of mineral oil per 100 grams of body weight, intraperitoneally. Twenty-four rats were divided into 4 groups and lipopolysaccharide (LPS, E. coli O111:B4) was injected intraperitoneally in 3 groups in a dose of 200 microgram/kg, 400 microgram/kg, and 800 microgram/kg, respectively. Expression of tissue factor was evaluated by real time PCR method. SYBR Green I, a DNA binding fluorophore was used for the detection of PCR product.


RESULTS
Expression of hepatic tissue factor tended to increase according to the amounts of LPS. Specially, the expression of tissue factor was significantly increased in the group into which 400 microgram/kg of LPS was injected, compared with control group (p<0.05).


CONCLUSIONS
Expression of tissue factor tends to increase according to the amounts of LPS in the persistent hepatic injury. LPS may play an important role in the expression of tissue factor in the CCl4-treated rat liver.
Cho
Mong
M
Department of Internal Medicine, Pusan National University College of Medicine, Seo-gu, Busan, Korea. mcho@pusan.ac.kr
Seo
Soo Boon
SB
Heo
Jeong
J
Kim
Gwang Ha
GH
Kang
Dae Hwan
DH
Song
Geun Am
GA
Yang
Ung Suk
US
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013925:Thromboplastin / Q000096:biosynthesis*
D004731:Endotoxins; C044101:endotoxin, Escherichia coli; D013925:Thromboplastin