A congenic strain (F344.OLETF-Imfm) displays the existence of intramuscular fat accumulation QTL on rat chromosome 1.
A genomic region between D1Wox8 and D1Rat90 on rat chromosome 1 was previously shown to be linked to intramuscular fat accumulation by quantitative trait locus (QTL) analysis using a F2 population derived from the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, which exhibits an increase in the levels of intramuscular fat content in Musculus longissimus, and the F344 rat. There exist two regions showing major and minor lod peaks for linkage to intramuscular fat accumulation, in the chromosomal region. We constructed a congenic strain introgressing the OLETF allele on the minor but not the major lod peak region in the F344 rat strain. The congenic strain had higher levels of intramuscular fat content in Musculus longissimus than the inbred partner F344 rat, thereby proving the existence of a QTL, designated Imfm (for Intramuscular fat-minor), responsible for the intramuscular fat accumulation in the congenic region of the minor lod peak region of about 10 cM. The F344.OLETF-Imfm congenic strain might provide a refined tool for the analysis of the gene causing intramuscular fat accumulation.
Tanomura
Hideki
H
Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyoku, Kyoto, Kyoto 606-8502, Japan.
Taniguchi
Yukio
Y
Muramatsu
Youji
Y
Kose
Hiroyuki
H
Miyake
Takeshi
T
Matsumoto
Kozo
K
Yamada
Takahisa
T
Sasaki
Yoshiyuki
Y
D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D020296:Animals, Congenic; D008126:Lod Score; D040641:Quantitative Trait Loci; D051381:Rats; D011916:Rats, Inbred F344; D020334:Rats, Inbred OLETF