Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect.
BACKGROUND
Mesenchymal stem cells from adult bone marrow are multipotent cells capable of forming bone, cartilage, and other connective tissues. In a previous study, we demonstrated that autologous mesenchymal stem cells could repair a critical-sized bone defect in the dog. The objective of this study was to determine whether the use of allogeneic mesenchymal stem cells could heal a critical-sized bone defect in the femoral diaphysis in dogs without the use of immunosuppressive therapy.


METHODS
A critical-sized segmental bone defect, 21 mm in length, was created in the mid-portion of the femoral diaphysis of twelve adult dogs that weighed between 22 and 25 kg. Each defect was treated with allogeneic mesenchymal stem cells loaded onto a hollow ceramic cylinder consisting of hydroxyapatite-tricalcium phosphate. A complete mismatch between donor stem cells and recipient dogs was identified by dog leukocyte antigen typing prior to implantation. The healing response was evaluated histologically and radiographically at four, eight, and sixteen weeks after implantation. The radiographic and histological results at sixteen weeks were compared with the historical data for the control defects, which included defects that had been treated with a cylinder loaded with autologous mesenchymal stem cells, defects treated with a cylinder without mesenchymal stem cells, and defects that had been left untreated (empty). The systemic immune response was evaluated by the analysis of recipient serum for production of antibodies against allogeneic cells.


RESULTS
For defects treated with allogeneic mesenchymal stem cell implants, no adverse host response could be detected at any time-point. Histologically, no lymphocytic infiltration occurred and no antibodies against allogeneic cells were detected. Histologically, by eight weeks, a callus spanned the length of the defect, and lamellar bone filled the pores of the implant at the host bone-implant interface. Fluorescently labeled allogeneic cells were also detected. At sixteen weeks, new bone had formed throughout the implant. These results were consistent with those seen in implants loaded with autologous cells. Implants loaded with allogeneic or autologous stem cells had significantly greater amounts of bone within the available pore space than did cell-free implants at sixteen weeks (p < 0.05).


CONCLUSIONS
The results of this study demonstrated that allogeneic mesenchymal stem cells loaded on hydroxyapatite-tricalcium phosphate implants enhanced the repair of a critical-sized segmental defect in the canine femur without the use of immunosuppressive therapy. No adverse immune response was detected in this model.
Arinzeh
Treena Livingston
TL
Osiris Therapeutics, Baltimore, Maryland, USA. arinzeh@njit.edu
Peter
Susan J
SJ
Archambault
Michael P
MP
van den Bos
Christian
C
Gordon
Steve
S
Kraus
Karl
K
Smith
Alan
A
Kadiyala
Sudha
S
D000818:Animals; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D001861:Bone Regeneration; D018483:Diaphyses / Q000000981:diagnostic imaging / Q000601:surgery / Q000648:ultrastructure; D004195:Disease Models, Animal; D004285:Dogs; D005269:Femur / Q000000981:diagnostic imaging / Q000601:surgery* / Q000648:ultrastructure; D008648:Mesoderm / Q000166:cytology* / Q000000981:diagnostic imaging; D011859:Radiography; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous