Inhibitory effect of trans-N-p-coumaroyl tryamine from the twigs of Celtis chinensis on the acetylcholinesterase.
The methanolic extract of the twigs of Celtis chinensis was found to show inhibitory activity on acetylcholinesterase (AChE), an enzyme that plays a role in the metabolic hydrolysis of ACh. Bioassay-guided fractionation of the methanolic extract resulted in the isolation of N-p-coumaroyl tyramine, as an inhibitor on AChE. This compound inhibited AChE activity in a dose-dependent manner, and the IC50 value of trans-N-p-coumaroyl tyramine was 34.5 microg/mL (122 microM).
Kim
Dae Keun
DK
College of Pharmacy, Woosuk University, Samrye 565-701, Korea. dkkim@woosuk.ac.kr
Lee
Kieseung
K
D000109:Acetylcholine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005544:Forecasting; D005625:Frontal Lobe / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008518:Medicine, East Asian Traditional; D000432:Methanol; D051379:Mice; D008813:Mice, Inbred ICR; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010946:Plants, Medicinal; D014439:Tyramine / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D034401:Ulmaceae
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; C487400:trans-N-p-coumaroyl tyramine; D000109:Acetylcholine; D014439:Tyramine; D000432:Methanol