title: Development of a liquid chromatography method for the determination of linezolid and its application to in vitro and human microdialysis samples.
abstract: Linezolid is a new, promising antibacterial agent to treat severe infections. A rapid HPLC assay using UV detection for the determination in microdialysate and human plasma was developed. After sample preparation, using acetonitrile for plasma and water for microdialysate, 20 microl was injected and separated on a RP-18 column. Overall, the assay exhibited good precision and accuracy. The diffusion properties of linezolid investigated in in vitro microdialysis experiments revealed a mean relative recovery of 77.5% (CV: 5.4%; delivery and recovery experiments). Following characterization of linezolid in in vitro microdialysis, the setting is suitable for application in clinical studies.
authors.affiliation: Department Clinical Pharmacy, Institute of Pharmacy, Freie Universitaet Berlin, Kelchstrasse 31, D-12169 Berlin, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Buerger
authors.forename: Christian
authors.initials: C
authors.lastname: Joukhadar
authors.forename: Markus
authors.initials: M
authors.lastname: Muller
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kloft
mesh_terms: D000081:Acetamides / Q000032:analysis* / Q000097:blood; D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D000069349:Linezolid; D017551:Microdialysis; D023303:Oxazolidinones / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D000069349:Linezolid