Synthesis of DNA conjugates by solid phase fragment condensation.
Development of a novel method for the synthesis of DNA conjugates is described. Oligonucleotides were successfully conjugated with a variety of functional molecules on a solid phase (Solid Phase Fragment Condensation) using an amino, a hydroxyl, a thiol, and a carboxyl group. DNA-peptide conjugate was obtained as a pure from by a single RPHPLC purification approximately in 20% yield. Moreover, it was demonstrated that the present method was effective for the preparation of conjugate molecules, DNA-sugar, DNA-polyamine, DNA-lipid and so on. The study to create new intelligent DNAs by accumulation various biofunctions on the molecule by SPFC is now in progress in our laboratory.
Anno
Yosuke
Y
Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, Iizuka, Fukuoka, Japan. yanno@iron.chem.fuk.kindai.ac.jp
Kubo
Takanori
T
Ueki
Ryoji
R
Yano
Mayuka
M
Sasaki
Kotomi
K
Ohba
Hideki
H
Fujii
Masayuki
M
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D004247:DNA