title: A long acidic domain affects the chromatographic behaviour of a neuronal adaptor protein on DEAE-Sepharose.
abstract: The stepwise chromatographic behaviour on DEAE-Sepharose of rat Fe65, a neuronal protein, was tested, using as eluants KCl, CaCl2, and MgCl2. Assays by western blot showed that Fe65 was eluted by CaCl2, at a ionic strength 20% lower than that of MgCl2 or KCl. Interestingly, in the case of a truncated Fe65, lacking a glutamic acid rich region at the N-terminus, the ionic strengths of the various eluants were almost identical. These results suggested a possible inhibitory role of calcium ions in the binding of the protein to DEAE and a specific affinity of these ions for long acidic stretches.
authors.affiliation: Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Italy.
authors.forename: Olimpia
authors.initials: O
authors.lastname: Longo
authors.forename: Annalisa
authors.initials: A
authors.lastname: Lamberti
authors.forename: Nicola
authors.initials: N
authors.lastname: Zambrano
authors.forename: Paolo
authors.initials: P
authors.lastname: Arcari
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002122:Calcium Chloride / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D015636:Magnesium Chloride / Q000737:chemistry / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D012685:Sepharose / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: C486242:Apbb1 protein, rat; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D015636:Magnesium Chloride; D012685:Sepharose; D002122:Calcium Chloride