Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer.
OBJECTIVE
To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer.


METHODS
Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 microg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA.


RESULTS
The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44+/-0.54 mg/g vs 1.45+/-0.52 mg/g mucosa, P<0.05). Duodenal RNA and protein contents did not differ between duodenal ulcer rats with and without EGF during the experimental period. Sham operation and duodenal ulcer rats with EGF significantly increased duodenal mucosal EGF content compared with those without EGF on day 5 (76.0+/-13.7 ng/g vs 35.7+/-12.9 ng/g mucosa in sham operation rats, and 68.3+/-10.9 ng/g vs 28.3+/-9.2 ng/g mucosa in duodenal ulcer rats, P<0.05).


CONCLUSIONS
Oral EGF can promote mucosal healing of the rats with duodenal ulcer by stimulating mucosal proliferation accompanied by an increase in mucosal EGF content.
Chao
Jane C J
JC
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan 110. chenjui@tmu.edu.tw
Liu
Kuo-Yu
KY
Chen
Sheng-Hsuan
SH
Fang
Chia-Lang
CL
Tsao
Chih-Wei
CW
D019342:Acetic Acid; D000284:Administration, Oral; D000818:Animals; D004247:DNA / Q000378:metabolism; D004381:Duodenal Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004435:Eating / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D008297:Male; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D011506:Proteins; D004815:Epidermal Growth Factor; D012313:RNA; D004247:DNA; D019342:Acetic Acid