Synthesis and hybridization properties of oligodeoxynucleotides containing 3'-deoxy-3'-C-methyleneuridine.
3'-Deoxy-3'-C-methyleneuridine nucleoside 1 has been incorporated into oligodeoxynucleotides. Relative to the unmodified references, oligomers containing nucleoside 1 displayed reduced binding affinities towards complementary DNA and RNA with a tendency towards RNA-selective hybridization.
Lindegaard
Dorthe
D
Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Odense M, Denmark.
Wengel
Jesper
J
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry*
C479258:3'-deoxy-3'-C-methyleneuridine; D018076:DNA, Complementary; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D014529:Uridine