title: Rice C2-domain proteins are induced and translocated to the plasma membrane in response to a fungal elicitor.
abstract: Hundreds of proteins involved in signaling pathways contain a Ca(2+)-dependent membrane-binding motif called the C2-domain. However, no small C2-domain proteins consisting of a single C2-domain have been reported in animal cells. We have isolated two cDNA clones, OsERG1a and OsERG1b, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library. The clones are believed to have originated from a single gene by alternative splicing. Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea or by Ca(2+) ions. The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca(2+)-dependent manner and is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Ca(2+) ionophore. These results suggest that OsERG1 proteins containing a single C2-domain are involved in plant defense signaling systems.
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju, Korea.
authors.forename: Cha Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Yoon Duck
authors.initials: YD
authors.lastname: Koo
authors.forename: Jing Bo
authors.initials: JB
authors.lastname: Jin
authors.forename: Byeong Cheol
authors.initials: BC
authors.lastname: Moon
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kang
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Byung Ouk
authors.initials: BO
authors.lastname: Park
authors.forename: So Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Man Lyang
authors.initials: ML
authors.lastname: Kim
authors.forename: Inhwan
authors.initials: I
authors.lastname: Hwang
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.forename: Jeong Dong
authors.initials: JD
authors.lastname: Bahk
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Moo Je
authors.initials: MJ
authors.lastname: Cho
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D020013:Calcium Signaling / Q000502:physiology; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D020082:Magnaporthe / Q000472:pathogenicity* / Q000502:physiology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport
chemical_list: D008565:Membrane Proteins; D010743:Phospholipids; D010940:Plant Proteins