Kinetic analysis of recombinant mammalian alpha(1) and alpha(1)beta glycine receptor channels.
To analyze the influence of the beta-subunit on the kinetic properties of GlyR channel currents, alpha(1)-subunits and alpha(1)beta-subunits were transiently expressed in HEK 293 cells. A piezo dimorph was used for fast application of glycine to outside-out patches. The rise time of activation was dose dependent for both receptors and decreased with increasing glycine concentrations. Subunit composition had no effect on the time course of activation. Coexpression of alpha(1)- and beta-subunits resulted in a significantly lower EC(50) and a reduced slope of the dose-response curve of glycine compared with expression of alpha(1)-subunits alone. For both receptor subtypes, the time course of desensitization was concentration dependent. Desensitization was best fitted with a single time constant at 10-30 micro M, with two at 0.1 mM, and at saturating concentrations (0.3-3 mM) with three time constants. Desensitization of homomeric alpha(1)-receptor channels was significantly slower than that of alpha(1)beta-receptor channels. The time course of current decay after the end of glycine pulses was tested at different pulse durations of 1 mM glycine. It was best fitted with two time constants for both alpha(1) and alpha(1)beta GlyR channels, and increased significantly with increasing pulse duration.
Mohammadi
B
B
Department of Neurology, Medical SchoolUniversity Hannover, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Krampfl
K
K
Cetinkaya
C
C
Moschref
H
H
Grosskreutz
J
J
Dengler
R
R
Bufler
J
J
D000818:Animals; D002460:Cell Line; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007668:Kidney / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D007700:Kinetics; D008322:Mammals; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D017434:Protein Structure, Tertiary; D018009:Receptors, Glycine / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
D018118:Chloride Channels; C114561:GLRB protein, human; D018009:Receptors, Glycine; D011994:Recombinant Proteins; D005998:Glycine