[Intracellular localization of XCAP-E protein in XL2 (Xenopus laevis) cells under normal conditions and during inhibition of pRNA transcription and processing].
The interaction of condensin subunit XCAP-E with various nucleolar subcompartments in XL2 cells was studied. In the interphase cells, XCAP-E was associated with a granular component of nucleoli (as shown by double staining with antibodies against B23) and with small nucleolus-like structures in the nucleoplasm. Inhibition of transcription by actinomycin D does not disrupt interaction of XCAP-E with the granular compartment of segregated nucleoli. Treatment with DRB 5,6-dichloro-1 beta-ribofuranozide-benzimidazole causes disintegration of nucleolar fibrillar complexes, but does not affect nucleolar localization of XCAP-E. The data suggest that nucleolar association of XCAP-E is independent on the functional state of the nucleolus, and imply a possible role of this protein in rRNA processing and pre-fibosome assembly.
Timirbulatova
E R
ER
Research Institute of Physico-Chemical Biology, Moscow State University, Institute of Gene Biology RAS, Moscow. al_mira@rambler.ru
Kireev
I I
II
Kartavenko
T V
TV
Gulak
P V
PV
Poliakov
V Iu
VIu
Guellec
K L
KL
Uzbekov
R E
RE
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000648:ultrastructure*; D018797:Cell Cycle Proteins; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D007399:Interphase; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000648:ultrastructure*; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000648:ultrastructure; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D029867:Xenopus Proteins; D014982:Xenopus laevis
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D014157:Transcription Factors; C090453:XCAP-E protein, Xenopus; D029867:Xenopus Proteins