title: Diagnosis of trichomoniasis in 'virgin' bulls by culture and polymerase chain reaction.
abstract: The diagnostic test for Tritrichomonas foetus in bulls is microscopic examination of cultured preputial samples. Trichomonads other than T. foetus can be present in a preputial sample. Both a staining technique and a polymerase chain reaction assay were useful in differentiating between T. foetus and another trichomonad observed in samples from virgin bulls.
authors.affiliation: Centre for Animal Parasitology, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Saskatchewan S7N 2R3.
authors.forename: Sarah
authors.initials: S
authors.lastname: Parker
authors.forename: John
authors.initials: J
authors.lastname: Campbell
authors.forename: Kathy
authors.initials: K
authors.lastname: McIntosh
authors.forename: Alvin
authors.initials: A
authors.lastname: Gajadhar
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003937:Diagnosis, Differential; D005837:Genitalia, Male / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity; D013194:Staining and Labeling / Q000662:veterinary; D014244:Trichomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014245:Trichomonas Infections / Q000175:diagnosis / Q000662:veterinary*