Polymorphism at the esterase isozyme locus Est10 associated with phylogenetic differentiation in rice.
A new esterase isozyme locus, Est10, with 6 alleles including the null form, has been found in rice by using polyacrylamide gel electrophoresis. Thirty F(2) populations of all possible combinations between 5 different band morphs were studied. The segregation pattern indicated that bands 1, 2, 3, 4, and the null form (0) were allelic with each other. The alleles of Est10 were distributed at different frequencies among different varietal groups of rice and also between cultivated rice and its wild relatives (Oryza rufipogon Griff.). Alleles 1 and 2 were frequently found in Japonica and Indica types, respectively. Allele 3 showed a high frequency in Aus and Boro, both Indica types cultivated in South Asia. Allele 4 was frequent in wild rice O. rufipogon. Judging from the linkage between Est10 and RFLP marker RG220 and isozyme marker Est5, Est10 is located on chromosome 1. The importance of this locus in evolutionary studies of rice is discussed.
Cai
Hongwei
H
Department of Plant Genetics and Breeding, College of Plant Science and Technology, China Agricultural University, Beijing, China. hcai@jfsass.or.jp
Zhang
Yaozhong
Y
Xu
Jichen
J
Zhu
Lihuang
L
Cheng
Kansheng
K
Wang
Xiangkun
X
D005075:Biological Evolution; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D004950:Esterases / Q000235:genetics*; D005787:Gene Frequency; D017343:Genes, Plant; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics; D012275:Oryza / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D007527:Isoenzymes; D004950:Esterases