title: Linear polyethylenimine as a tool for comparative studies of antisense and short double-stranded RNA oligonucleotides.
abstract: Despite the recently enlarged field of available RNA knock-down technologies, e.g., antisense oligonucleotides (ASOs) and duplexes of synthetic 21 nucleotides RNAs (siRNAs), no versatile transfection reagent has been reported to deliver different nucleic acids formats at high rates of efficiency. We have evaluated the versatility and efficacy of linear PEI in transfecting and properly delivering a broad panel of nucleic acids such as short oligonucleotides and double-stranded RNA into cells in culture.
authors.affiliation: Functional Genomics, Novartis Pharma AG, Basel, Switzerland.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Bologna
authors.forename: Gabriele
authors.initials: G
authors.lastname: Dorn
authors.forename: Francois
authors.initials: F
authors.lastname: Natt
authors.forename: Jan
authors.initials: J
authors.lastname: Weiler
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D007202:Indicators and Reagents; D011094:Polyethyleneimine / Q000494:pharmacology*; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D016372:RNA, Antisense / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000235:genetics; D058477:Receptors, Purinergic P2X3; D014162:Transfection
chemical_list: D007202:Indicators and Reagents; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D012313:RNA; D011094:Polyethyleneimine