Heparanase-1 gene expression in normal, hyperplastic and neoplastic prostatic tissue.
Heparanase-1 (Hpa-1) has been implicated in tumour invasion and metastasis. In the present study, we evaluated the clinicopathological significance of Hpa-1 mRNA expression in prostate cancer and non-cancerous prostatic tissue by one-step polymerase chain reaction (PCR) of laser microdissected prostatic gland cells. In addition, cell type-specific expression of Hpa-1 mRNA in prostatic tissue was analysed by in situ hybridisation. Hpa-1 mRNA expression was found in 50% of normal and 40% of hyperplastic prostatic tissue. In situ hybridisation showed that Hpa-1 mRNA was strongly expressed in prostate gland cells. Of the 26 prostate carcinomas tested, 42% were positive for Hpa-1 mRNA. However, in non-cancerous prostatic tissue, Hpa-1 mRNA was significantly more often expressed than in less differentiated or more invasive prostate cancers (P<0.05). In situ hybridisation revealed only focal Hpa-1 mRNA expression in the neoplastic gland cells. Hpa-1 mRNA expression in the tumours significantly correlated with tumour differentiation and tumour stage (P<0.05). Our data indicate that Hpa-1 gene expression may be lost during dedifferentiation of prostatic gland cells.
Stadlmann
S
S
Institute of Pathology, University of Innsbruck, Muellerstrasse 44, 6020 Innsbruck, Austria. sylvia.stadlmann@uibk.ac.at
Moser
P L
PL
Pollheimer
J
J
Steiner
P
P
Krugmann
J
J
Dirnhofer
S
S
Mikuz
G
G
Margreiter
R
R
Amberger
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015870:Gene Expression; D019763:Heparin Lyase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D019763:Heparin Lyase