Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser.
The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200-300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9x10(-8) M and 4.5x10(-8) M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800+/-85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.
Miao
Hai
H
Department of Chemistry and the Beckman Institute, University of Illinois, Urbana, 61801, USA.
Rubakhin
Stanislav S
SS
Sweedler
Jonathan V
JV
D000818:Animals; D001048:Aplysia; D004558:Electric Stimulation; D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D009474:Neurons / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014466:Ultraviolet Rays
D012701:Serotonin