Construction of a gorilla fosmid library and its PCR screening system.
A gorilla fosmid library of 261,120 independent clones was constructed and characterized. The fosmid vector is similar to the cosmid in average insert size of ca. 40 kb but contains the F factor for replication, and it is more resistant to recombination. This clone library represents about 3.7 times coverage of the gorilla genome. A simple screening system by PCR was established, and we successfully found 9 clones that cover the entire Hox A gene cluster of the gorilla genome. This gorilla fosmid DNA library is a useful resource for comparative genomics of human and apes.
Kim
Choong Gon
CG
Division of Population Genetics, National Institute of Genetics, Mishima 411-8540, Japan.
Fujiyama
Asao
A
Saitou
Naruya
N
D000818:Animals; D003360:Cosmids; D005260:Female; D015698:Genomic Library; D006071:Gorilla gorilla / Q000235:genetics*; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*