[Study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography].
The extract solution from Sweet Potato was washed with 5% Na2CO3 and followed with distilled water and dried with dehydrated sodium sulfate (Na2SO4). The ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel G thin-layer plate developed with different solvent systems. Pure ipomeamarone and ipomeamaronol were used as standards. The resolution was the best in the solvent system of petroleum ether-ethylacetate at 20 degrees C. The separated spots were scanned by CS-930 TLC scanner at 520 nm and quantitative determination by external standard calibration. Ipomeamarone was detected by high performance thin-layer chromatography and the limits of detection were 0.002 microgram for pure and 0.01 microgram for crude sample.
Shi
Qun
Q
School of Public Health, Wuhan University, Wuhan 430071, China.
Tan
Xiaodong
X
Mao
Zongfu
Z
Tao
Ning
N
Yi
Yourong
Y
D002855:Chromatography, Thin Layer; D027723:Ipomoea batatas / Q000382:microbiology*; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification; D012717:Sesquiterpenes / Q000032:analysis
D009183:Mycotoxins; D012717:Sesquiterpenes; C001469:ipomeamarone