title: [Effects of soybean isoflavone and calcium on proliferation, differentiation and mineralization of cultured osteoblasts in vitro].
abstract: To explore the effects of soybean isoflavone and calcium on proliferation, differentiation and mineralization of cultured osteoblastic cells. Osteoblastic cells from newborn rat calvarial was cultured. Osteoblastic cells proliferation was measured by MTT, activity of APL was observed with Golden's method and ARS was used to measure the mineral nodes of osteoblasts. The results showed that the calcium group had not different from the control group on stimulating the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells. The soybean isoflavone group had significant effects on stimulating the proliferation, differentiation of osteoblasts and increase the forming of the mineral nodes of osteoblasts in rat. Calcium and soybean isoflavone group had more benefit for forming mineral nodes. The soybean isoflavone stimulates the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells, and increase calcium in the same time is more benefit for mineralization.
authors.affiliation: Molecular Biology Laboratory, Xinxiang Medical College, Xinxiang 453003, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Tian
authors.forename: Xianjun
authors.initials: X
authors.lastname: Yang
authors.forename: Wanli
authors.initials: W
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Hanjun
authors.initials: H
authors.lastname: Yue
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007529:Isoflavones / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology; D013025:Glycine max / Q000737:chemistry*
chemical_list: D007529:Isoflavones; D000469:Alkaline Phosphatase; D002118:Calcium