Histological and mechanical investigation of the bone-bonding ability of anodically oxidized titanium in rabbits.
The purpose of this study was to histologically and mechanically investigate the in vivo bone-bonding ability of anodically oxidized titanium (AO Ti) with an anatase crystal layer on its surface. AO Ti plates, anodically oxidized at 155 V in 1 M H2SO4, were implanted into the proximal metaphyses of mature rabbit tibiae for 4, 8, 16, and 24 weeks and investigated by light microscopy, scanning electron microscopy and detaching test. High bone-bonding ability, comparable to our previous study data of the bioactive titanium produced by sodium-free alkali and heat treatment, was observed at the early stages of implantation. However, no substantial increase was demonstrated. AO Ti plates bonded to bone directly, with no intervening soft tissue layer, and no breakage of the AO Ti layer was observed. The AO Ti layer was porous through to the titanium substrate, while the porosity was low. Apatite-like deposition into the pores of the AO layer was observed only in the superficial zone. The lack of improvement of bone-bonding ability in the later stages of implantation may be attributed to the low porosity and to the superficial ingrowths of apatite-like deposits into the pores of the AO Ti layer.
Liang
Bojian
B
Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Shogoin, Kawahara-cho 54, Sakyo-ku, Kyoto 606-8507, Japan.
Fujibayashi
Shunsuke
S
Neo
Masashi
M
Tamura
Jiro
J
Kim
Hyun-Min
HM
Uchida
Masaki
M
Kokubo
Tadashi
T
Nakamura
Takashi
T
D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D004566:Electrodes; D008422:Materials Testing / Q000379:methods*; D016348:Osseointegration / Q000502:physiology*; D010084:Oxidation-Reduction; D011817:Rabbits; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013977:Tibia / Q000166:cytology* / Q000502:physiology* / Q000601:surgery / Q000648:ultrastructure; D014025:Titanium / Q000737:chemistry*
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium