HLA class II antigen expression in conjunctival precancerous lesions and squamous cell carcinomas.
OBJECTIVE
The expression of human leukocyte antigen (HLA) class II molecules on the cell surface is necessary for the presentation of peptide antigens to helper CD4 T lymphocytes of the immune system. We studied the immunoexpression of HLA class II antigen in conjunctival precursor lesions and conjunctival squamous cell carcinomas.


METHODS
HLA class II antigen expression was analyzed in 8 conjunctival dysplasias, 6 carcinomas in situ and in 7 conjunctival squamous cell carcinomas, by immunoperoxidase staining with monoclonal antibody to HLA class II antigen on the archival clinical samples. Immunoanalysis was done by a semi quantitative method based on the intensity of staining and the percentage of stained cells.


RESULTS
HLA class II antigen immunoexpression was heterogeneous in 8 conjunctival dysplasias and in 6 carcinoma in situ and negative in 7 conjunctival squamous cell carcinomas.


CONCLUSIONS
Human leukocyte class II antigen immunoexpression is decreased in conjunctival precancerous and squamous cell carcinomas.
Abhyankar
Dhiraj
D
Department of Medicine, Evanston Northwestern Healthcare, Evanston, Illinois, USA
Lakshmi
Sundaram Amirtha
SA
Pushparaj
Vaijayanthi
V
Biswas
Jyotirmay
J
Krishnakumar
Subramanian
S
D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D003230:Conjunctival Neoplasms / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D011230:Precancerous Conditions / Q000276:immunology*; D013194:Staining and Labeling
D000949:Histocompatibility Antigens Class II