The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures.
The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7- O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7- O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7- O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside.
Koulman
Albert
A
Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), University of Groningen, Groningen, The Netherlands. A.Koulman@farm.rug.nl
Beekman
Aäron C
AC
Pras
Niesko
N
Quax
Wim J
WJ
D002478:Cells, Cultured / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D019597:Flax / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008517:Phytotherapy; D011034:Podophyllotoxin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*
D004365:Drugs, Chinese Herbal; C014451:deoxypodophyllotoxin; D011034:Podophyllotoxin