Detection and evaluation of NO stores in awake rats.
We demonstrated and substantiated the possibility of detection and evaluation of NO stores in freely moving awake rats. NO stores were created by administering NO donor or by heat shock and were then detected by hypotensive reaction to diethyldithiocarbamate (blood pressure monitoring) under conditions of NO synthase inhibition. Electron paramagnetic resonance revealed NO release from its stores by incorporation into paramagnetic mononitrosyl-iron complexes with diethyldithiocarbamate. Five hours after administration of NO donor or heat shock diethyldithiocarbamate induced a blood pressure drop and the appearance of electron paramagnetic resonance signals from the mononitrosyl-iron-diethyldithiocarbamate complex in rat heart, liver, kidneys, and brain. The hypotensive reaction to diethyldithiocarbamate and electron paramagnetic resonance signals were absent in control rats.
Mashina
S Yu
SY
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow.
Vanin
A F
AF
Serezhenkov
V A
VA
Kubrina
L N
LN
Malenkova
I V
IV
Malyshev
I Yu
IY
Manukhina
E B
EB
D000818:Animals; D001794:Blood Pressure; D002614:Chelating Agents / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006358:Hot Temperature; D007022:Hypotension; D007501:Iron / Q000737:chemistry; D008956:Models, Chemical; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D013997:Time Factors; D014851:Wakefulness
D002614:Chelating Agents; D009569:Nitric Oxide; D004050:Ditiocarb; D007501:Iron; D019001:Nitric Oxide Synthase