title: Multivariate exploratory tools for microarray data analysis.
abstract: The ultimate success of microarray technology in basic and applied biological sciences depends critically on the development of statistical methods for gene expression data analysis. The most widely used tests for differential expression of genes are essentially univariate. Such tests disregard the multidimensional structure of microarray data. Multivariate methods are needed to utilize the information hidden in gene interactions and hence to provide more powerful and biologically meaningful methods for finding subsets of differentially expressed genes. The objective of this paper is to develop methods of multidimensional search for biologically significant genes, considering expression signals as mutually dependent random variables. To attain these ends, we consider the utility of a pertinent distance between random vectors and its empirical counterpart constructed from gene expression data. The distance furnishes exploratory procedures aimed at finding a target subset of differentially expressed genes. To determine the size of the target subset, we resort to successive elimination of smaller subsets resulting from each step of a random search algorithm based on maximization of the proposed distance. Different stopping rules associated with this procedure are evaluated. The usefulness of the proposed approach is illustrated with an application to the analysis of two sets of gene expression data.
authors.affiliation: Huntsman Cancer Institute and Department of Oncological Sciences, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112-5550, USA. aniko.szabo@hci.utah.edu
authors.forename: Aniko
authors.initials: A
authors.lastname: Szabo
authors.forename: Kenneth
authors.initials: K
authors.lastname: Boucher
authors.forename: David
authors.initials: D
authors.lastname: Jones
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Tsodikov
authors.forename: Lev B
authors.initials: LB
authors.lastname: Klebanov
authors.forename: Andrei Y
authors.initials: AY
authors.lastname: Yakovlev
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D016001:Confidence Intervals; D003627:Data Interpretation, Statistical; D016002:Discriminant Analysis; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D006801:Humans; D007938:Leukemia / Q000235:genetics; D015999:Multivariate Analysis; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data*; D011336:Probability; D018709:Statistics, Nonparametric