title: Tn5060 from the Siberian permafrost is most closely related to the ancestor of Tn21 prior to integron acquisition.
abstract: A Tn21-related mercury resistance transposon, Tn5060, has been isolated from Pseudomonas sp. strain A19-1 from a 8,000-10,000-year-old Siberian permafrost sample, and sequenced. Like Tn21, the element transposes to different plasmids at a frequency of 10(-2)-10(-3) per target plasmid transfer. Comparison of the complete Tn5060 DNA sequence (8,667 bp) with that of Tn21 (19,672 bp) shows that Tn5060 does not contain integron In2 and deviates from Tn21 in four nucleotide positions. These and other comparative data demonstrate that Tn5060 is the most closely related of the characterized mercury resistances to the as yet hypothetical immediate ancestor of Tn21, TnX.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia. kholodii@img.ras.ru
authors.forename: Gennady
authors.initials: G
authors.lastname: Kholodii
authors.forename: Sofia
authors.initials: S
authors.lastname: Mindlin
authors.forename: Mayya
authors.initials: M
authors.lastname: Petrova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Minakhina
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D023061:Gene Order; D005798:Genes, Bacterial; D032023:Integrons; D008628:Mercury / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009876:Operon; D011549:Pseudomonas / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia; D012988:Soil Microbiology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008628:Mercury