title: Polyethylene glycol additives reduce hemolysis in red blood cell suspensions exposed to mechanical stress.
abstract: Mechanical damage to blood cells is of considerable concern in the development and use of circulatory assist devices and other blood contacting systems. Furthermore, hemodilution with saline, dextran, and other plasma expanders applied during extracorporeal circulation and dialysis increases red blood cell (RBC) susceptibility to the high shear stresses associated with these procedures. In this paper, we present polyethylene glycol (PEG) as a potential erythrocyte protective agent against mechanically induced cellular trauma. Bovine RBCs were subjected to mechanical stress induced by rolling stainless steel shots through RBC suspensions for a constant exposure time. The suspensions were prepared at a hematocrit of 30% in various media: PEG (20,000 molecular weight), autologous bovine plasma, Dextran 40 solution, and phosphate buffered saline (PBS). RBC suspensions in Dextran 40 were prepared at a viscosity similar to the PEG suspensions. We found the hemolysis level of RBCs suspended in plasma and in PEG solutions to be several times lower (p < 0.001) than in the Dextran and PBS solutions. No statistically significant difference was found between the hemolysis that occurred in suspensions of RBCs in autologous plasma and in 2.0% PEG solutions. Even PEG concentration as low as 0.1% reduced hemolysis by more than 40% compared with PBS or the same concentration of Dextran in suspension medium. Our data demonstrate the efficacy of PEG molecules in reducing mechanical trauma to erythrocytes and suggest the potential for using PEG in assisted circulation, dialysis, and other procedures where RBCs are subjected to extensive mechanical stress.
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Surgery, University of Pittsburgh, 3025 East Carson St., Pittsburgh, PA 15203, USA.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Kameneva
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Repko
authors.forename: Ellen F
authors.initials: EF
authors.lastname: Krasik
authors.forename: Brett C
authors.initials: BC
authors.lastname: Perricelli
authors.forename: Harvey S
authors.initials: HS
authors.lastname: Borovetz
mesh_terms: D000818:Animals; D001187:Artificial Organs; D002417:Cattle; D004912:Erythrocytes / Q000187:drug effects*; D005112:Extracorporeal Circulation; D006438:Hemodilution; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013314:Stress, Mechanical
chemical_list: D011092:Polyethylene Glycols