title: Somatic mutation in mitochondrial DNA and nuclear microsatellite instability in gastric cancer.
abstract: It was reported that somatic mutations in the mitochondrial DNA (mtDNA) are associated with high-frequency microsatellite instability (MSI-H) of the nuclear in gastric cancers. However, no correlation between mtDNA mutations and nuclear MSI-H was found in colorectal, breast, and renal cancers. Therefore, the association between mtDNA mutations and nuclear MSI-H in gastric cancers is controversial. We examined mtDNA mutations and nuclear MSI in a large panel of gastric cancers. One-hundred and five gastric cancers were selected. Mutations in the mononucleotide repeat (D310) of mtDNA and nuclear MSI at 5 microsatellite loci were examined by microsatellite assay. Somatic mutations in the mtDNA and nuclear MSI-H were detected in 16 (15%) and 14 (13%) of the gastric cancers, respectively. mtDNA mutations were detected in 2 of the 14 (14%) and 14 of the 91 (15%) tumors with and without nuclear MSI-H, respectively. There was no significant difference between them. These results suggest that somatic mutations in the mtDNA and nuclear MSI-H play important roles in gastric carcinogenesis, and that mtDNA mutations may not be associated with nuclear MSI-H in gastric cancers.
authors.affiliation: Health Service Center, Hiroshima University, Higashihiroshima 739-8521, Japan. tohiyama@hiroshima-u.ac.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Hiyama
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kose
authors.forename: Handan
authors.initials: H
authors.lastname: Tuncel
authors.forename: Masanori
authors.initials: M
authors.lastname: Ito
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kitadai
authors.forename: Masaharu
authors.initials: M
authors.lastname: Sumii
authors.forename: Masaharu
authors.initials: M
authors.lastname: Yoshihara
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimamoto
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D004247:DNA