CD40-targeted adenoviral GM-CSF gene transfer enhances and prolongs the maturation of human CML-derived dendritic cells upon cytokine deprivation.
Vaccination with autologous leukaemia-derived dendritic cells (DC) presents an adjuvant treatment option for chronic myeloid leukaemia (CML). Here, we show that high-efficiency CD40-targeted adenoviral gene transfer of GM-CSF to CML-derived DC induces long-lived maturation in the absence of exogenous cytokines and may thus ensure protracted stimulation of CML-specific T cells upon vaccination.
Stam
A G M
AG
Department of Pathology, Free University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands.
Santegoets
S J A M
SJ
Westers
T M
TM
Sombroek
C C
CC
Janssen
J J W M
JJ
Tillman
B W
BW
van de Loosdrecht
A A
AA
Pinedo
H M
HM
Curiel
D T
DT
Ossenkoppele
G J
GJ
Scheper
R J
RJ
de Gruijl
T D
TD
D000256:Adenoviridae / Q000235:genetics*; D019013:CD40 Antigens / Q000235:genetics* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D019013:CD40 Antigens; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor