title: Evaluation of capillary HPLC/mass spectrometry as an alternative analysis method for gel electrophoresis of oligonucleotides.
abstract: A method has been developed to monitor the enzymatic incorporation of nucleotides in DNA by electrospray HPLC mass spectrometry. The main advantages of mass spectrometry over electrophoresis are the ability to directly characterize the reaction products and the shorter analysis time.
authors.affiliation: Rega Institute for Medical Research, K.U. Leuven, Leuven, Belgium. jef.rozenski@rega.kuleuven.ac.be
authors.forename: Jef
authors.initials: J
authors.lastname: Rozenski
authors.forename: Karen
authors.initials: K
authors.lastname: Vastmans
authors.forename: Arthur
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D001483:Base Sequence; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D004247:DNA