Improved PCR for detection of the highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans in subgingival plaque samples.
The JP2 clone of Actinobacillus actinomycetemcomitans is associated with early-onset periodontitis in certain ethnic populations of African origin. Here, we describe and evaluate a set of primers for PCR to assay for the presence of A. actinomycetemcomitans and to discriminate between JP2-like strains and other genotypes in subgingival plaque samples.
Poulsen
Knud
K
Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark. kp@microbiology.au.dk
Ennibi
Oum-Keltoum
OK
Haubek
Dorte
D
D000189:Actinobacillus Infections / Q000382:microbiology; D000293:Adolescent; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010520:Aggressive Periodontitis / Q000382:microbiology; D017931:DNA Primers; D003773:Dental Plaque / Q000382:microbiology*; D005098:Exotoxins / Q000235:genetics* / Q000378:metabolism; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D005098:Exotoxins; C018579:leukotoxin