title: Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis.
abstract: Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.
authors.affiliation: Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia, Australia. tim.inglis@health.wa.gov.au
authors.forename: Timothy J J
authors.initials: TJ
authors.lastname: Inglis
authors.forename: Max
authors.initials: M
authors.lastname: Aravena-Roman
authors.forename: Simon
authors.initials: S
authors.lastname: Ching
authors.forename: Kevin
authors.initials: K
authors.lastname: Croft
authors.forename: V
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Mee
mesh_terms: D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000737:chemistry / Q000145:classification*; D016957:Burkholderia pseudomallei / Q000737:chemistry / Q000145:classification* / Q000472:pathogenicity; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D019814:Myristic Acid / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D019814:Myristic Acid