title: Sequence analysis of segment a of a field virus isolate from an outbreak of Infectious bursal disease in India.
abstract: Sequence analysis of genome segment A of an Indian Infectious bursal disease virus (IBDV) field isolate (KT1/99) revealed total 95 nucleotide substitutions, resulting in 17 amino acid changes. Of these, five amino acid changes, namely F60S, T137I, I374V, V519I and E682D were unique to the KT1/99 isolate. The amino acid change P222A and the proposed hot mutation spot 680Y, reported to be present in very virulent IBDV isolates were also found in KT1/99. This isolate had nucleotide divergence of 1.1% to 4.95% from the other reported serotype 1 IBDV isolates and 19.6% from serotype 2 strain OH in polyprotein gene sequence, while divergence at amino acid level was 0.6% to 2.9% and 11.4%, respectively. Based on both nucleotide and amino acid sequence analysis, KT1/99 was grouped phylogenetically with the reported Bangladesh isolate BD3/99 in one cluster along with other reported very virulent isolates in same lineage.
authors.affiliation: National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar, 243122 UP, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Bais
authors.forename: R S
authors.initials: RS
authors.lastname: Kataria
authors.forename: A K
authors.initials: AK
authors.lastname: Tiwari
authors.lastname: Indervesh
authors.forename: V V S
authors.initials: VV
authors.lastname: Suryanarayana
authors.forename: S K
authors.initials: SK
authors.lastname: Das
mesh_terms: D000595:Amino Acid Sequence; D018175:Birnaviridae Infections / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks; D015684:Gene Products, pol / Q000235:genetics*; D016679:Genome, Viral; D007194:India / Q000453:epidemiology; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D015684:Gene Products, pol