PEP-19 overexpression in human uterine leiomyoma.
Although uterine leiomyomas represent one of the most common neoplasms in adult women, their pathogenesis remains poorly understood. A cDNA microarray analysis was performed to search for candidate genes expressed to a greater degree in leiomyoma compared with matched myometrium. A total of 15 candidate genes was obtained; neuron-specific protein PEP-19 (Purkinje cell protein 4; PCP 4) exhibited a striking difference in expression between leiomyoma and myometrium. Although PEP-19 expression has been reported exclusively in the central nervous system, the present study demonstrated that PEP-19 is also expressed in other human organs, including prostate, kidney and uterus. To clarify the role of PEP-19 in the pathogenesis of leiomyomas, PEP-19 expression was investigated for a series of human leiomyoma, as well as normal myometrium and leiomyosarcoma. PEP-19 mRNA and protein expression were much stronger in leiomyomas compared with normal myometrium, suggesting that PEP-19 might be involved in leiomyoma pathogenesis.
Kanamori
Takanobu
T
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Takakura
Kenji
K
Mandai
Masaki
M
Kariya
Masatoshi
M
Fukuhara
Ken
K
Kusakari
Takashi
T
Momma
Chika
C
Shime
Hiroaki
H
Yagi
Haruhiko
H
Konishi
Mitsunaga
M
Suzuki
Ayako
A
Matsumura
Noriomi
N
Nanbu
Kanako
K
Fujita
Jun
J
Fujii
Shingo
S
D000328:Adult; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D008875:Middle Aged; D009215:Myometrium / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C050394:PCP4 protein, human