title: Distribution of the MCS4 RNA genes in mycoplasmas belonging to the Mycoplasma mycoides cluster.
abstract: MCS4 RNA is one of the small stable RNAs found in Mycoplasma capricolum subsp. capricolum type strain California kid. This RNA has a sequence similarity to that of eukaryotic U6 snRNA. There are two genes encoding MCS4 RNA, designated mcs4a and mcs4b, in the genome. Homologous sequences of these genes were not found in databases of other bacterial sequences. We searched for MCS4 RNA and its genes in other bacteria by PCR and hybridization techniques. The results strongly suggested that this RNA exists only in a limited species of mycoplasmas belonging to the Mycoplasma mycoides cluster.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Aomori 036-8561, Hirosaki, Japan. cushida@cc.hirosaki-u.ac.jp
authors.forename: Chisato
authors.initials: C
authors.lastname: Ushida
authors.forename: Ayumi
authors.initials: A
authors.lastname: Yoshida
authors.forename: Yukie
authors.initials: Y
authors.lastname: Miyakawa
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ara
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D022661:RNA, Untranslated