title: Gamma-butyrolactone autoregulators and receptor proteins in non- Streptomyces actinomycetes producing commercially important secondary metabolites.
abstract: The presence of gamma-butyrolactone autoregulators and their receptor proteins were investigated in five representative strains of non- Streptomyces actinomycetes producing commercially important secondary metabolites. Ethyl acetate extracts of culture were assayed using wild-type S. virginiae for virginiae butanolide, S. lavendulae FRI-5 for IM-2, and S. griseus HH1 for A-factor. Actinoplanes teichomyceticus and Amycolatopsis mediterranei were shown to produce autoregulators. Corresponding autoregulator-binding activities were found in the crude cell-free lysates of these strains, using the binding assay with tritium-labeled autoregulator analogues as ligands, which suggests that non- Streptomyces actinomycetes might have autoregulator-dependent signaling cascades.
authors.affiliation: International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan.
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Choi
authors.forename: Chang-Kwon
authors.initials: CK
authors.lastname: Lee
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Hwang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kinosita
authors.forename: Takuya
authors.initials: T
authors.lastname: Nihira
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism; D006706:Homeostasis; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: C037481:4-butyrolactone receptor; D006133:Growth Substances; D011963:Receptors, GABA-A; D015107:4-Butyrolactone