title: Improved procedure for the determination of malonaldehyde by gas-chromatography with electron-capture detection as 2,4,6-trichlorophenylhydrazine derivative.
abstract: A previously described derivatization method using trichlorophenylhydrazine was developed for the estimation of malonaldehyde measured by gas-chromatography (GC) with electron-capture detection. The precision and reliability of the procedure are improved here by the use of methylmalonaldehyde as internal standard and by the introduction of a diverter valve at the end of the capillary column to protect the electron-capture detector, respectively. The method was applied to determine malonaldehyde content in bovine plasma samples.
authors.affiliation: Department of Veterinary Science and Technology for Food Safety, Faculty of Veterinary Medicine, University of Milan, Via Celoria 10, Milan 20133, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Sangalli
authors.forename: Luca Maria
authors.initials: LM
authors.lastname: Chiesa
authors.forename: Elena
authors.initials: E
authors.lastname: Passerò
authors.forename: Ada
authors.initials: A
authors.lastname: Manzocchi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maffeo
authors.forename: Pier Antonio
authors.initials: PA
authors.lastname: Biondi
mesh_terms: D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006834:Hydrazines; D008315:Malondialdehyde; C071044:2,4,6-trichlorophenylhydrazine