Covalent coupling of a PIM-1 oncogene targeted PNA with an antennapedia derived peptide.
Peptide nucleic acids (PNA) are promising antisense molecule for blocking gene expression in cell culture or in vivo. Nevertheless because they are poor efficient to pass the cellular membrane, it is necessary to use a vectorisation agent to observe an inhibitory effect. We describe the coupling of the rhodamine labeled 17-mer antisense PNA to a fusogenic peptide from antenapedia via S-S linkage, the studies of the penetration of this complex into fibroblast cells and its inhibitory effect on pim1 targeted protononcogene.
Bertrand
J R
JR
CNRS, UMR 8121, Institut Gustave Roussy, Villejuif cedex, France. irb@igr.fr
Sumbatyan
N
N
Malvy
C
C
D016475:3T3 Cells; D000818:Animals; D051943:Antennapedia Homeodomain Protein; D001483:Base Sequence; D018398:Homeodomain Proteins / Q000494:pharmacology; D051379:Mice; D009687:Nuclear Proteins; D009857:Oncogenes / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology; D020135:Peptide Nucleic Acids / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D011519:Proto-Oncogenes / Q000187:drug effects; D014157:Transcription Factors
D051943:Antennapedia Homeodomain Protein; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D020135:Peptide Nucleic Acids; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; C496636:Pim1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1