Inhibition of gene expression by peptide nucleic acids in cultured cells.
We have tested in cultured cells the capacity of antisense and antigene PNAs to inhibit, in a sequence specific manner, the expression of oncogenes in leukaemia and pancreatic carcinoma cells. The results observed appeared promising and suggest that PNA may play in the future an important role in targeting disease-related genes.
Cogoi
Susanna
S
Department of Biochemical Sciences and Technologies, Udine, Italy.
Rapozzi
Valentina
V
Xodo
Luigi E
LE
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002460:Cell Line; D003062:Codon / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000493:pharmacokinetics / Q000494:pharmacology; D020135:Peptide Nucleic Acids / Q000493:pharmacokinetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D003062:Codon; D020319:Oligodeoxyribonucleotides, Antisense; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger