Molecular cloning and characterization of a novel leptospiral lipoprotein with OmpA domain.
A novel antigenic protein of pathogenic Leptospira, Loa22, was identified by using the PhoA fusion method followed by immunoblotting with convalescent mouse sera. Loa22 was shown to be a lipoprotein having a C-terminal OmpA consensus domain. Loa22 was detected among pathogenic leptospires but not among non-pathogenic leptospires, suggesting the possible involvement of this protein in virulence. The results of three different experiments suggested that Loa22 is located in the outer membrane and a small portion is exposed on the cell surface. Thus, Loa22 may be a candidate for a novel vaccine against infection with pathogenic leptospires.
Koizumi
Nobuo
N
Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Watanabe
Haruo
H
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines; D003001:Cloning, Molecular; D015698:Genomic Library; D015151:Immunoblotting; D007919:Leptospira / Q000737:chemistry* / Q000235:genetics* / Q000472:pathogenicity; D007922:Leptospirosis / Q000517:prevention & control; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D011993:Recombinant Fusion Proteins; C078167:OMPA outer membrane proteins