A transcriptional pause synchronizes translation with transcription in the tryptophanase operon leader region.
Regulation of transcription of the tryptophanase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its transcription. We show in vitro that a transcription pause site exists at the end of the tnaC coding region and that translation of tnaC releases the paused transcription complex, coupling transcription with translation.
Gong
Feng
F
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA.
Yanofsky
Charles
C
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014368:Tryptophanase / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C081473:tnaC protein, E coli; D014368:Tryptophanase