Detection of hepatitis C virus RNA in formalin-fixed, paraffin-embedded thin-needle liver biopsy specimens.
We examined the feasibility of detecting hepatitis C virus (HCV) RNA in formalin-fixed, paraffin-embedded (FFPE) specimens obtained by thin-needle biopsy (TNB). Specimens obtained by large-needle biopsy (LNB) and unfixed frozen tissue served as controls. A total of 23 biopsy specimens, 13 obtained by TNB and 10 by LNB, from 20 patients with chronic hepatitis C were included in the study. HCV RNA was detected by nested reverse transcription-polymerase chain reaction (RT-PCR). HCV RNA was found in FFPE archival specimens obtained by TNB (11 [85%]) or LNB (7 [70%]). The sensitivity was similar in unfixed tissue in which HCV RNA was found in 7 (88%) of 8 TNB specimens and 6 (86%) of 7 LNB specimens. The detection of HCV RNA did not seem to be affected by storage of the paraffin blocks, the presence of advanced fibrosis and cirrhosis, or fragmentation of the core cylinder. TNB yields enough qualitative suitable material to detect HCV RNA by RT-PCR.
Vogt
Stefanie
S
Department of Pathology, Otto-von-Guericke University, Leipziger Str 44, D-39120 Magdeburg, Germany.
Schneider-Stock
Regine
R
Klauck
Sabine
S
Roessner
Albert
A
Röcken
Christoph
C
D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D017931:DNA Primers / Q000737:chemistry; D005240:Feasibility Studies; D005260:Female; D005557:Formaldehyde; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000175:diagnosis / Q000821:virology*; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016707:Tissue Fixation / Q000379:methods
D017931:DNA Primers; D012367:RNA, Viral; D005557:Formaldehyde