title: Interactions between centromere complexes in Saccharomyces cerevisiae.
abstract: We have purified two new complexes from Saccharomyces cerevisiae, one containing the centromere component Mtw1p together with Nnf1p, Nsl1p, and Dsn1p, which we call the Mtw1p complex, and the other containing Spc105p and Ydr532p, which we call the Spc105p complex. Further purifications using Dsn1p tagged with protein A show, in addition to the other components of the Mtw1p complex, the two components of the Spc105p complex and the four components of the previously described Ndc80p complex, suggesting that all three complexes are closely associated. Fluorescence microscopy and immunoelectron microscopy show that Nnf1p, Nsl1p, Dsn1p, Spc105p, and Ydr532p all localize to the nuclear side of the spindle pole body and along short spindles. Chromatin immunoprecipitation assays show that all five proteins are associated with centromere DNA. Homologues of Nsl1p and Spc105p in Schizosaccharomyces pombe also localize to the centromere. Temperature-sensitive mutations of Nsl1p, Dsn1p, and Spc105p all cause defects in chromosome segregation. Synthetic-lethal interactions are found between temperature-sensitive mutations in proteins from all three complexes, in agreement with their close physical association. These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, England.
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Nekrasov
authors.forename: Melanie A
authors.initials: MA
authors.lastname: Smith
authors.forename: Sew
authors.initials: S
authors.lastname: Peak-Chew
authors.forename: John V
authors.initials: JV
authors.lastname: Kilmartin
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002503:Centromere; D018386:Kinetochores; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics; D020539:Sequence Analysis, Protein; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C120362:MTW1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013205:Staphylococcal Protein A; C492315:mis12 protein, S pombe