title: Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.
abstract: Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.
authors.affiliation: Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France. maxime.dahan@lkb.ens.fr
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
authors.forename: Sabine
authors.initials: S
authors.lastname: Lévi
authors.forename: Camilla
authors.initials: C
authors.lastname: Luccardini
authors.forename: Philippe
authors.initials: P
authors.lastname: Rostaing
authors.forename: Béatrice
authors.initials: B
authors.lastname: Riveau
authors.forename: Antoine
authors.initials: A
authors.lastname: Triller
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D004058:Diffusion; D005453:Fluorescence; D005456:Fluorescent Dyes; D008854:Microscopy, Electron; D036103:Nanotechnology; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D018009:Receptors, Glycine / Q000378:metabolism*; D012666:Semiconductors; D013116:Spinal Cord / Q000166:cytology; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure
chemical_list: C092350:FM 4-64; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D018009:Receptors, Glycine