title: High-throughput screening of structural proteomics targets using NMR.
abstract: We applied a high-throughput strategy for the screening of targets for structural proteomics of Xanthomonas axonopodis pv citri. This strategy is based on the rapid (1)H-(15)N HSQC NMR analysis of bacterial lysates containing selectively (15)N-labelled heterologous proteins. Our analysis permitted us to classify the 19 soluble candidates in terms of 'foldedness', that is, the extent to which they present a well-folded solution structure, as reflected by the quality of their NMR spectra. This classification allowed us to define a priority list to be used as a guide to select protein candidates for further structural studies.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-900, SP, São Paulo, Brazil.
authors.forename: Leonor M P
authors.initials: LM
authors.lastname: Galvão-Botton
authors.forename: Angela M
authors.initials: AM
authors.lastname: Katsuyama
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Guzzo
authors.forename: Fábio C L
authors.initials: FC
authors.lastname: Almeida
authors.forename: Chuck S
authors.initials: CS
authors.lastname: Farah
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Valente
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D005798:Genes, Bacterial; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D016366:Open Reading Frames; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012995:Solubility; D014974:Xanthomonas / Q000737:chemistry* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins