Synthesis and triplex binding properties of oligonucleotides containing a novel nucleobase.
The thiazolo-indole compound 1 bearing the complementary donor-acceptor-donor sites (dad) was designed for specific recognition of an AT inverted base pair in pyrimidine triple helix motif. It was successfully incorporated into 14-mer oligonucleotide using a serinol unit as sugar derivative. The triple helix hybridization studies were examined by means of thermal denaturation experiments with a 26-mer DNA duplex containing the AT inverted base pair.
Lecubin
Florence
F
Institut de Chimie des Substances Naturelles, CNRS, 91198, Gif sur Yvette, France.
Devys
Michel
M
Fourrey
Jean Louis
JL
Sun
Jian-Sheng
JS
Benhida
Rachid
R
D000225:Adenine; D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D003855:Deoxyribose / Q000031:analogs & derivatives*; D006860:Hydrogen Bonding; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013941:Thymine
D009838:Oligodeoxyribonucleotides; D003855:Deoxyribose; D000225:Adenine; D013941:Thymine