title: Identification and expression analysis of a novel splice variant of human Sprouty1 gene.
abstract: Sprouty (SPRY) was first identified in a genetic screen in Drosophila to be an antagonist of fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling, seemingly by inhibiting the Ras/MAP kinase pathway. During large scale DNA sequencing of the human fetal brain cDNA library, we cloned a novel splice variant of human Sprouty1 gene, and termed it human Sprouty1b (SPRY1b). It has the same deduced protein as Sprouty1a, which has been reported. And like other members of Sprouty family, SPRY1b deduced protein also has a C-terminal cysteine-rich region. According to the search against human genome database, SPRY1b was mapped to 4q25-28. Expression analysis of SPRY1a and SPRY1b shows that they are hardly expressed in adult human, but have different expression patterns in fetus, which confirmed that SPRY1 is an important gene during fetal development.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Hai
authors.initials: H
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Qihan
authors.initials: Q
authors.lastname: Wu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Dai
authors.forename: Gang
authors.initials: G
authors.lastname: Yin
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D010750:Phosphoproteins; C486055:SPRY1 protein, human; C425468:Spry1 protein, mouse