Study of the interaction of 6-mercaptopurine with protein by microdialysis coupled with LC and electrochemical detection based on functionalized multi-wall carbon nanotubes modified electrode.
Microdialysis sampling coupled with liquid chromatography and electrochemical detection (LC-ECD) was developed and applied to study the interaction of 6-Mercaptopurine (6-MP) with bovine serum albumin (BSA). In the LC-ECD, the multi-wall carbon nanotubes fuctionalized with carboxylic groups modified electrode (MWNT-COOH CME) was used as the working electrode for the determination of 6-MP. The results indicated that this chemically modified electrode (CME) exhibited efficiently electrocatalytic oxidation for 6-MP with relatively high sensitivity, stability and long-life. The peak currents of 6-MP were linear to its concentrations ranging from 4.0 x 10(-7) to 1.0 x 10(-4) mol l(-1) with the calculated detection limit (S/N = 3) of 2.0 x 10(-7) mol l(-1). The method had been successfully applied to assess the association constant (K) and the number of the binding sites (n) on a BSA molecular, which calculated by Scatchard equation, were 3.97 x 10(3) mol(-1) l and 1.51, respectively. This method provided a fast, sensible and simple technique for the study of drug-protein interactions.
Cao
Xu-Ni
XN
Department of Chemistry, East China, Normal University, Shanghai 200062, China.
Lin
Li
L
Zhou
Yu-Yan
YY
Zhang
Wen
W
Shi
Guo-Yue
GY
Yamamoto
Katsunobu
K
Jin
Li-Tong
LT
D002384:Catalysis; D002853:Chromatography, Liquid; D004563:Electrochemistry; D004566:Electrodes; D007202:Indicators and Reagents; D015122:Mercaptopurine / Q000737:chemistry*; D017551:Microdialysis; D008854:Microscopy, Electron; D036103:Nanotechnology; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry
D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine; D015122:Mercaptopurine