Hemispheric dissociation of the involvement of NOS isoforms in memory for discriminated avoidance in the chick.
Previous research has indicated a role for both the neuronal (nNOS) and endothelial (eNOS) nitric oxide isoforms in memory formation. In addition, two distinct periods of activity of nitric oxide activity, dissociated by hemispheric localization, are implicated following passive avoidance training in the chick. In the present study, we trained black Australorp-white Leghorn chicks on a color discrimination avoidance task. Diphenyleneiodonium chloride (1 microM) or N-propyl-l-arginine (50 microM) was administered into either the left or right hemisphere of the chick brain in an attempt to differentiate the effects of inhibiting eNOS or nNOS, respectively. The memory loss previously observed following administration of diphenyleneiodonium chloride between 10 and 20 min posttraining was found to be lateralized to the right hemisphere, although administration of this agent into the left hemisphere around the time of training was also amnestic. In contrast, N-propyl-l-arginine caused memory loss only when administered to the left hemisphere around the time of training. These findings suggest that activation of both eNOS and nNOS isoforms may be essential for long-term memory consolidation of this task. Further, these two periods of activity are defined temporally and by hemisphere localization, although confirmation with more selective inhibitors when they become available is advised.
Rickard
Nikki S
NS
Department of Psychology, Monash University, Victoria 3145, Australia. n.rickard@med.monash.edu.au
Gibbs
Marie E
ME
D000647:Amnesia / Q000503:physiopathology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001362:Avoidance Learning / Q000502:physiology*; D001921:Brain / Q000201:enzymology / Q000502:physiology*; D002645:Chickens; D003118:Color Perception / Q000502:physiology; D004193:Discrimination Learning / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007839:Functional Laterality; D007527:Isoenzymes; D008568:Memory / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000502:physiology*; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; D009861:Onium Compounds / Q000494:pharmacology
D004791:Enzyme Inhibitors; D007527:Isoenzymes; C109599:N(omega)-propylarginine; D009861:Onium Compounds; C007517:diphenyleneiodonium; D001120:Arginine; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III