Induced genomic instability in irradiated germ cells and in the offspring; reconciling discrepancies among the human and animal studies.
Many studies confirmed that radiation induces genomic instability in whole-body systems. However, the results of the studies are not always consistent with each other. Attempts are made in the present review to resolve the discrepancies. Many of the studies in human and experimental animals utilize the length change mutation of minisatellite sequences as a marker of genomic instability. Minisatellite sequences frequently change their length, and the data obtained by conventional Southern blotting give rather qualitative information, which is sometimes difficult to scrutinize quantitatively. This is the problem inevitably associated with the study of minisatellite mutations and the source of some conflicts among studies in humans and mice. Radiation induction of genomic instability has also been assessed in whole-body experimental systems, using other markers such as the mouse pink-eyed unstable allele and the specific pigmentation loci of medaka fish (Oryzias latipes). Even though there are some contradictions, all these studies have demonstrated that genomic instability is induced in the germ cells of irradiated parents, especially of males, and in offspring born to them. Among these, transmission of genomic instability to the second generation of irradiated parents is limited to the mouse minisatellite system, and awaits further clarification in other experimental systems.
Niwa
Ohtsura
O
Radiation Biology Center, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan. tsimura@house.rbc.kyoto-u.ac.jp
D000818:Animals; D002352:Carrier Proteins; D004249:DNA Damage; D004330:Drosophila / Q000528:radiation effects; D042822:Genomic Instability / Q000528:radiation effects*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D051379:Mice; D018598:Minisatellite Repeats / Q000235:genetics; D009154:Mutation; D009990:Oryzias / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis / Q000528:radiation effects; D013094:Spermatozoa / Q000528:radiation effects
D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C489600:OCA2 protein, human; C076239:P protein, mouse