Synthesis of CCK-8 tetrapeptide fragment by enzymatic method.
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.
Xiang
Guangya
G
Pharmacy School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Eckstein
Heiner
H
D002384:Catalysis; D002918:Chymotrypsin; D009842:Oligopeptides; D010206:Papain; D010446:Peptide Fragments; D012844:Sincalide / Q000138:chemical synthesis*
D009842:Oligopeptides; D010446:Peptide Fragments; D002918:Chymotrypsin; D010206:Papain; D012844:Sincalide