Inhibitory effect of polypeptides from Chlamys farreri on UVB-induced apoptosis and DNA damage in normal human dermal fibroblasts in vitro.
OBJECTIVE
To investigate the effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B (UVB)-induced apoptosis and DNA damage in cultured normal human dermal fibroblasts.


METHODS
MTT assay was used to measure the viability of cells. Measurements of apoptosis and cytosolic free [Ca2+]i were performed with flow cytometry. The comet assay was employed to detect DNA damage in individual cell.


RESULTS
PCF (0.25 %-1%) greatly enhanced the proliferative capacity of cultured fibroblasts irradiated by UVB (1.176 x 10(-4) J/cm2) and markedly reduced apoptosis and the level of DNA damage in a concentration-dependent manner. Meanwhile, PCF could decrease the cytosolic free [Ca2+]i (P<0.01, compared with UVB model).


CONCLUSIONS
The inhibitory effect of PCF on UVB-induced photoaging is due to enhanced abating of UVB-injured DNA and UVB-induced apoptosis. Therefore, PCF can resist UV-induced aging development at the initiation stage.
Ding
Bo-Xiao
BX
Department of Pharmacology, Medical College, Qingdao University, Qingdao 266021, China.
Wang
Chun-Bo
CB
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology; D010125:Ostreidae / Q000737:chemistry; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology; D011897:Random Allocation; D014466:Ultraviolet Rays / Q000009:adverse effects*
D008419:Materia Medica; D010455:Peptides; D020011:Protective Agents