Dual regulated RNA transport pathways to the cortical region in developing rice endosperm.
Prolamine and glutelin RNAs are localized to two subdomains of the cortical endoplasmic reticulum (ER), the protein body ER and the cisternal ER, in developing rice seeds. The addition of nearly full-length prolamine sequences at the 3' untranslated region of a reporter RNA redirects its localization from the cisternal ER to the protein body ER. Deletion analysis of prolamine RNA sequences indicates the presence of two partially redundant cis elements required for protein body ER targeting. The addition of glutelin 3' untranslated region to protein body ER cis sequences, however, redirects RNA localization to the cisternal ER. These results indicate that there are at least two regulated RNA transport pathways as well as a constitutive pathway to the cortical ER.
Hamada
Shigeki
S
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
Ishiyama
Keiki
K
Sakulsingharoj
Chotipa
C
Choi
Sang-Bong
SB
Wu
Yujia
Y
Wang
Changlin
C
Singh
Salvinder
S
Kawai
Naoko
N
Messing
Joachim
J
Okita
Thomas W
TW
D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D055315:Prolamins; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D010940:Plant Proteins; D055315:Prolamins; D018749:RNA, Plant