Novel method for the covalent immobilization of oligonucleotides via Diels-Alder bioconjugation.
The synthesis of cyclohexadiene and maleimide derivatives and their use for the functionalization of oligonucleotides and the coating of glass surfaces is reported. A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented.
Latham-Timmons
Hallie A
HA
Proligo LLC, Boulder, Colorado 80301, USA.
Wolter
Andreas
A
Roach
J Shawn
JS
Giare
Rubina
R
Leuck
Michael
M
D002625:Chemistry, Organic / Q000379:methods; D005898:Glass; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D013499:Surface Properties
D015345:Oligonucleotide Probes; D009841:Oligonucleotides