Interactive effects of jasmonic acid, salicylic acid, and gibberellin on induction of trichomes in Arabidopsis.
Leaf trichomes protect plants from attack by insect herbivores and are often induced following damage. Hormonal regulation of this plant induction response has not been previously studied. In a series of experiments, we addressed the effects of artificial damage, jasmonic acid, salicylic acid, and gibberellin on induction of trichomes in Arabidopsis. Artificial damage and jasmonic acid caused significant increases in trichome production of leaves. The jar1-1 mutant exhibited normal trichome induction following treatment with jasmonic acid, suggesting that adenylation of jasmonic acid is not necessary. Salicylic acid had a negative effect on trichome production and consistently reduced the effect of jasmonic acid, suggesting negative cross-talk between the jasmonate and salicylate-dependent defense pathways. Interestingly, the effect of salicylic acid persisted in the nim1-1 mutant, suggesting that the Npr1/Nim1 gene is not downstream of salicylic acid in the negative regulation of trichome production. Last, we found that gibberellin and jasmonic acid had a synergistic effect on the induction of trichomes, suggesting important interactions between these two compounds.
Traw
M Brian
MB
Department of Ecology and Evolution, University of Chicago, 1101 E. 57th Street, Chicago, Illinois 60637, USA.
Bergelson
Joy
J
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D022081:Cell Surface Extensions / Q000187:drug effects / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005875:Gibberellins / Q000494:pharmacology; D009154:Mutation; D054883:Oxylipins; D019441:Plant Epidermis / Q000187:drug effects / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology; D013314:Stress, Mechanical
D003517:Cyclopentanes; D005875:Gibberellins; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D020156:Salicylic Acid