Improved procedure for the determination of malonaldehyde by gas-chromatography with electron-capture detection as 2,4,6-trichlorophenylhydrazine derivative.
A previously described derivatization method using trichlorophenylhydrazine was developed for the estimation of malonaldehyde measured by gas-chromatography (GC) with electron-capture detection. The precision and reliability of the procedure are improved here by the use of methylmalonaldehyde as internal standard and by the introduction of a diverter valve at the end of the capillary column to protect the electron-capture detector, respectively. The method was applied to determine malonaldehyde content in bovine plasma samples.
Sangalli
Lorenzo
L
Department of Veterinary Science and Technology for Food Safety, Faculty of Veterinary Medicine, University of Milan, Via Celoria 10, Milan 20133, Italy.
Chiesa
Luca Maria
LM
Passerò
Elena
E
Manzocchi
Ada
A
Maffeo
Giovanni
G
Biondi
Pier Antonio
PA
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006834:Hydrazines; D008315:Malondialdehyde; C071044:2,4,6-trichlorophenylhydrazine