[Antimicrobial susceptibility testing of bacteria isolated from animals: methods for in-vitro susceptibility testing and their suitability with regard to the generation of the most useful data for therapeutic applications].
In-vitro susceptibility testing provides valuable informations for choosing the most suitable antimicrobial agent for the control of bacterial infections in animals. Different diffusion and dilution methods, as conducted according to various approved performance standards, can be used to determine the in-vitro susceptibility of bacterial pathogens. In the present article, problems are discussed which arise from the use of different methods and the difficulty to interpret such results. While most approved performance standards were designed for testing of bacteria from human sources, the NCCLS document M31-A2 exclusively focusses on susceptibility testing of bacteria isolated from animals and--in contrast to all other standards--includes veterinary specific breakpoints for a number of antimicrobial agents used in veterinary medicine. Therefore, performance of in-vitro susceptibility testing of veterinary pathogens should follow the recommendations given in the NCCLS document M31-A2. The microdilution method is recommended as the method of choice for susceptibility testing. The result of a microdilution test is given as the minimum inhibitory concentration (MIC). This value provides a quantitative result which precisely indicates the degree of susceptibility of the tested bacterial strain and in return gives the veterinarian a clear guidance whether therapeutic intervention with the antibiotic in question will be successful.
Schwarz
Stefan
S
Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Neustadt-Mariensee. stefan.schwarz@fal.de
Böttner
Alexander
A
Hafez
Hafez M
HM
Kehrenberg
Corinna
C
Kietzmann
Manfred
M
Klarmann
Dieter
D
Klein
Günter
G
Krabisch
Peter
P
Kühn
Tilman
T
Luhofer
Gabriele
G
Richter
Angelika
A
Traeder
Wolfgang
W
Waldmann
Karl-Heinz
KH
Wallmann
Jürgen
J
Werckenthin
Christiane
C
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy / Q000662:veterinary*; D015169:Colony Count, Microbial / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000662:veterinary*; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents