[Genetic polymorphism of 8 STR loci on short arm of chromosome 3].
OBJECTIVE
To get the genotype and allele frequency distributions of 8 short tandem repeat (STR) loci on chromosome 3p (D3S1297, D3S1489, D3S1266, D3S1568, D3S1289, D3S1300, D3S1285 and D3S3681) in Chinese Han population in Hunan area.


METHODS
Blood samples were collected from the random Han individuals in Hunan and the whole genomic DNA was extracted. STR loci were amplified by multiplex-PCR technique and genotyped by ABI 377 sequencer.


RESULTS
Ninety-one alleles were detected, with frequencies ranging from 0.002 to 0.431, and these alleles constituted 312 genotypes. All the 8 loci met Hardy-Weinberg equilibrium. The statistical analysis of 8 STR loci showed the heterozygosity (H) >or= 0.729, the discrimination power (DP) >or= 0.725, the probabilities of paternity exclusion (PPE) >or= 0.596, and the polymorphic information content (PIC >or= 0.682). The result indicated that there was a significant difference between Han ethnic group and the white and the black.


CONCLUSIONS
These results could serve as valuable data to enrich the Chinese genetic database and play an important role in Chinese population genetic and forensic medical application.
Xiong
Wei
W
Cancer Research Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 PR China.
Zeng
Zhao-yang
ZY
Xiong
Fang
F
Shen
Shou-rong
SR
Li
Xiao-ling
XL
Li
Wei-fang
WF
Wang
Rong
R
Fan
Song-qing
SQ
Yang
Yi-xin
YX
Zhou
Hou-de
HD
Zhou
Ming
M
Li
Gui-yuan
GY
D000328:Adult; D000483:Alleles; D002681:China; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic