title: Morphofunctional studies on human labial salivary glands.
abstract: In this study, the first experimental investigation carried out at the ultrastructural level on mucous cells of human salivary glands, we have examined by light microscopy (LM), transmission electron microscopy (TEM), high resolution scanning electron microscopy (HRSEM), the secretory response of labial glands stimulated in vitro by the beta-adrenergic agent, D,L isoproterenol, and by the muscarinic agent carbachol. For comparison we have used identical methods to study samples of mixed portions of human submandibular glands. Morphological findings obtained here on both submandibular and labial glands mucous cells demonstrate that mucous droplets are released solely by muscarinic stimulation, and that cytological events occurring during secretory discharge are similar to those described by others, using TEM, on stimulated mucous cells of rat sublingual glands. Despite the fact that human labial glands are said to have a prominent cholinergic innervation with scanty adrenergic nerves, the response of seromucous cells in these organs to stimulation with carbachol and with isoproterenol was similar to that observed by us, (using LM, TEM and HRSEM), in serous cells of human major salivary glands.
authors.affiliation: Department of Cytomorphology, University of Cagliari, Cittadella Universitaria, Cagliari, Monserrato, Italy. riva@unica.it
authors.forename: A
authors.initials: A
authors.lastname: Riva
authors.forename: R
authors.initials: R
authors.lastname: Puxeddu
authors.forename: F
authors.initials: F
authors.lastname: Loy
authors.forename: F
authors.initials: F
authors.lastname: Testa Riva
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008853:Microscopy; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009092:Mucous Membrane / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001285:Atropine; D002217:Carbachol; D011433:Propranolol; D007545:Isoproterenol; D010646:Phentolamine