Preimplantation aneuploidy screening using comparative genomic hybridization or fluorescence in situ hybridization of embryos from patients with recurrent implantation failure.
OBJECTIVE
To select chromosomally euploid embryos for transfer by analyzing single biopsied blastomeres using either fluorescence in situ hybridization (FISH) for chromosomes 13, 16, 18, 21, and 22 or comparative genomic hybridization (CGH), which provides a full karyotype.


METHODS
Prospective observational study.


METHODS
A large IVF unit and the research laboratory of a hospital clinical genetics unit.


METHODS
Twenty patients with recurrent implantation failure.


METHODS
Ovarian stimulation and IVF by intracytoplasmic sperm injection (ICSI), embryo biopsy, and embryo transfer.


METHODS
Chromosome normality of biopsied blastomeres and implantation and clinical pregnancy rates.


RESULTS
Comparative genomic hybridization was able to identify many chromosomal abnormalities that would have been missed if those cells had been analyzed by FISH. The clinical pregnancy rate per transfer and implantation rate was 11% and 7% for embryos analyzed by FISH and 21% and 15% for embryos analyzed by CGH.


CONCLUSIONS
Comparative genomic hybridization is more effective than FISH for identifying chromosomally normal embryos, which may result in a higher clinical pregnancy rate and implantation rate after embryo transfer.
Wilton
Leeanda
L
Melbourne IVF, Freemason's Hospital Medical Centre, East Melbourne, Victoria, Australia. lwilton@mivf.com.au
Voullaire
Lucille
L
Sargeant
Peta
P
Williamson
Robert
R
McBain
John
J
D000328:Adult; D000782:Aneuploidy; D015925:Cryopreservation; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods; D011446:Prospective Studies; D012008:Recurrence; D017211:Treatment Failure