Analysis of human fecal material for autosomal and Y chromosome STRs.
Human stool samples from eight volunteers were stored under various conditions and extracted by three different procedures. Fecal material and tissue paper soiled with fecal material obtained from a crime scene were also extracted. Extracted DNA was amplified using the AmpFlSTR Profiler Plus, AmpFlSTR COfiler, and the AmpFlSTR Identifiler PCR amplification kits for the detection of the autosomal STR allelic patterns. DNA extracted from the male volunteers and from the soiled tissue paper evidence sample was also amplified using the Y-PLEX 6 and Y-PLEX 5 amplification kits. Analysis of the amplified products was carried out by capillary electrophoresis on the ABI PRISM 310 Genetic Analyzer. Autosomal and Y-STR profiles obtained from the fecal material were concordant with the results from the donors' buccal swabs.
Roy
Reena
R
St. Louis County Police Crime Laboratory, 111 South Meramec, Clayton, MO 63105, USA. rroy@stlouisco.com
D000483:Alleles; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D019075:Electrophoresis, Capillary; D005243:Feces / Q000737:chemistry*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences
D004247:DNA