Evaluating the potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species.
The potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species was evaluated. A set of 25 barley and 3 wheat microsatellite markers were tested for their ability to cross-amplify DNA from four accessions of E. trachycaulus and two accessions Pseudoroegneria spicata. Thirteen barley (52%) and two (68%) wheat primer pairs successfully amplified consistent products from both E. trachycaulus and P. spicata species. Four of the 15 successful primer pairs produced visible polymorphisms among the accessions tested. A higher successful rate of cross-species amplification of barley and wheat microsatellite markers in E. trachycaulus and P. spicata was found in this study. These primer pairs are now available for use as markers in genetic analysis of E. trachycaulus complex species. Our results suggest that publicly available wheat and barley microsatellite markers are a valuable resource for the genetic characterization of wild Triticeae species.
MacRitchie
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Biology Department, Saint Mary's University, Halifax, Nova Scotia, B3H 3C3, Canada.
Sun
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D017931:DNA Primers; D031749:Elymus / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers