Isolation and characterization of solventogenic, cellulase-free xylanolytic Clostridia from cow rumen.
Hemicellulolytic obligate anaerobes capable of converting a range of agricultural substrates and monomeric sugars of hemicellulose to solvents and acids were isolated from cow rumen fluid. The strains were identified as 5 strains of C. beijerinckii (3 different genotypes), 3 strains of C. acetobutylicum (2 different genotypes), 1 strain of C. butyricum and 3 strains of C. bifermentans (2 different genotypes). All of them produced cellulase-free xylanase activity. C. acetobutylicum LU1 produced solvent, a high of 5.1 gl(-1) and C.bifermentans LU1 produced xylanase activity a high of 4.2 IU ml(-1).
Sankar
M
M
Department of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
Delgado
O
O
Mattiasson
B
B
D000818:Animals; D018533:Biomass; D002417:Cattle; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D005838:Genotype; D011134:Polysaccharides / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D012997:Solvents; D014995:Xylosidases / Q000494:pharmacology
D011134:Polysaccharides; D012997:Solvents; C007916:hemicellulose; D014995:Xylosidases