title: Conventional and micellar liquid chromatography method development for danazol and validation in capsules.
abstract: Two isocratic liquid chromatographic methods (conventional and micellar) for the determination of danazol (DZ) in capsules using canrenone (CAN) as internal standard have been developed and validated. In conventional liquid chromatography a mobile phase 35% water:acetonitrile 65%, v:v, a flow-rate 1 ml min(-1) and a C18 Hypersil ODS (250 x 4.6 mm, 5 microm) column (25 degrees C) were used. In micellar liquid chromatography (MLC) the conditions were: mobile phase 40 mM sodium dodecyl sulfate:2% pentanol, flow-rate 0.5 ml min(-1) and C18 Hypersil ODS (150 x 3.0 mm, 5 microm) column (60 degrees C). For both methods. UV absorbance detection at 280 nm was used and a separation up to base line was achieved. Prior to HPLC analysis a simple sample preparation was required. The recoveries found in the accuracy test were 99 +/- 10 and 101 +/- 8%, in conventional liquid chromatography (CLC) and MLC, respectively. Repeatability and intermediate precision expressed as R.S.D. were lower than 5% for both methods. Detection limits obtained were 2.4 and 3.0 ng g(-1) in CLC and CLM, respectively.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense, Ciudad Universitaria, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Gonzalo-Lumbreras
authors.forename: R
authors.initials: R
authors.lastname: Izquierdo-Hornillos
mesh_terms: D002192:Canrenone / Q000032:analysis; D002214:Capsules; D020374:Chromatography, Micellar Electrokinetic Capillary; D003613:Danazol / Q000032:analysis*; D004965:Estrogen Antagonists / Q000032:analysis*; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002214:Capsules; D004965:Estrogen Antagonists; D007202:Indicators and Reagents; D002192:Canrenone; D003613:Danazol