title: Influence of the length of the phosphate chain in mRNA 5' cap analogues on their interaction with eukaryotic initiation factor 4E.
abstract: The recognition of the 5'mRNA cap structure m7G(5')ppp(5')N by one of the components of the initiation translation machinery, the eIF4E factor, plays a pivotal role in regulation of the protein synthesis. In the present study we have shown two opposing roles of the cap phosphate chain in the specific eIF4E-cap interaction. The extension of the phosphate chain enhances the binding of the cap to the unphosphorylated eIF4E but destabilises the eIF4E-cap complex in case of the phosphorylated protein.
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zuberek
authors.forename: Jacek
authors.initials: J
authors.lastname: Jemielity
authors.forename: Anna
authors.initials: A
authors.lastname: Niedzwiecka
authors.forename: Janusz
authors.initials: J
authors.lastname: Stepinski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Wyslouch-Cieszynska
authors.forename: Ryszard
authors.initials: R
authors.lastname: Stolarski
authors.forename: Edward
authors.initials: E
authors.lastname: Darzynkiewicz
mesh_terms: D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D007700:Kinetics; D010442:Peptide Chain Initiation, Translational; D010710:Phosphates; D011485:Protein Binding; D012315:RNA Caps / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010710:Phosphates; D012315:RNA Caps