Analysis of parental strain DNA fragments existing in GEMs-Fhhh.
There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs (genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp1, mnp2, lip1, lip2, FLO1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.
Hao
Chun-Bo
CB
The National Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science, Nanjing University, Nanjing 210093, China.
Yan
Jun
J
Qu
Meng-Meng
MM
Wang
Dong
D
Cheng
Shu-Pei
SP
Gu
Ji-Dong
JD
Qiu
Wan-Fei
WF
Wang
Yin-Yin
YY
D001419:Bacteria / Q000235:genetics; D001673:Biodegradation, Environmental; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005818:Genetic Engineering; D007218:Industrial Microbiology; D030781:Organisms, Genetically Modified / Q000235:genetics*; D020075:Phanerochaete / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics
D017931:DNA Primers