title: Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale.
abstract: This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
authors.affiliation: Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Valdés
authors.forename: Biunayki
authors.initials: B
authors.lastname: Reyes
authors.forename: Tatiana
authors.initials: T
authors.lastname: Alvarez
authors.forename: Janet
authors.initials: J
authors.lastname: García
authors.forename: José A
authors.initials: JA
authors.lastname: Montero
authors.forename: Alejandro
authors.initials: A
authors.lastname: Figueroa
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gómez
authors.forename: Sigifredo
authors.initials: S
authors.lastname: Padilla
authors.forename: Déborah
authors.initials: D
authors.lastname: Geada
authors.forename: Ma Carmen
authors.initials: MC
authors.lastname: Abrahantes
authors.forename: Lamay
authors.initials: L
authors.lastname: Dorta
authors.forename: Damián
authors.initials: D
authors.lastname: Fernández
authors.forename: Otto
authors.initials: O
authors.lastname: Mendoza
authors.forename: Nadia
authors.initials: N
authors.lastname: Ramirez
authors.forename: Marylin
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Merardo
authors.initials: M
authors.lastname: Pujol
authors.forename: Carlos
authors.initials: C
authors.lastname: Borroto
authors.forename: José
authors.initials: J
authors.lastname: Brito
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D017325:Hepatitis B Vaccines / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000276:immunology*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D008024:Ligands; D010940:Plant Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic