title: Identification of Pseudomonas proteins coordinately induced by acidic amino acids and their amides: a two-dimensional electrophoresis study.
abstract: The acidic amino acids (Asp, Glu) and their amides (Asn, Gln) are excellent growth substrates for many pseudomonads. This paper presents proteomics data indicating that growth of Pseudomonas fluorescens ATCC 13525 and Pseudomonas putida KT2440 on these amino acids as sole source of carbon and nitrogen leads to the induction of a defined set of proteins. Using mass spectrometry and N-terminal sequencing, a number of these proteins were identified as enzymes and transporters involved in amino acid uptake and metabolism. Most of them depended on the alternative sigma factor sigma(54) for expression and were subject to strong carbon catabolite repression by glucose and citrate cycle intermediates. For a subset of the identified proteins, the observed regulatory effects were independently confirmed by RT-PCR. The authors propose that the respective genes (together with others still to be identified) make up a regulon that mediates uptake and utilization of the abovementioned amino acids.
authors.affiliation: Philipps-University Marburg, Institute of Physiological Chemistry, D-35032 Marburg, Germany.
authors.forename: Avinash
authors.initials: A
authors.lastname: Sonawane
authors.affiliation: Philipps-University Marburg, Institute of Physiological Chemistry, D-35032 Marburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Klöppner
authors.affiliation: Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg, Germany.|Philipps-University Marburg, Department of Biology, Laboratory for Microbiology, D-35032, Marburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Hövel
authors.affiliation: Ernst-Moritz-Arndt-University, Medical Faculty, Laboratory for Functional Genomics, D-17487 Greifswald, Germany.|Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg, Germany.|Philipps-University Marburg, Department of Biology, Laboratory for Microbiology, D-35032, Marburg, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.affiliation: Philipps-University Marburg, Institute of Physiological Chemistry, D-35032 Marburg, Germany.
authors.forename: Klaus-Heinrich
authors.initials: KH
authors.lastname: Röhm
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis; D001216:Asparagine / Q000494:pharmacology*; D001224:Aspartic Acid / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018698:Glutamic Acid / Q000494:pharmacology*; D005972:Glutaminase / Q000096:biosynthesis; D005973:Glutamine / Q000494:pharmacology*; D019897:Periplasm / Q000201:enzymology; D040901:Proteomics; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012808:Sigma Factor / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D001216:Asparagine; D001215:Asparaginase; D005972:Glutaminase