title: Inhibitory and promotive effects of polyamines on transglutaminase-induced protein polymerization.
abstract: Transglutaminase (TGase) has been reported to be involved in the regulation of cell growth. We examined the effects of polyamines on TGase activity. The polymerization of casein was inhibited by putrescine (PUT) and spermidine (SPD). On the other hand, polymerization of N,N-dimethylcasein was increased by spermine (SPM) and SPD. These results suggested polyamines played two distinct roles as inhibitor and promoter for TGase-catalyzed protein polymerization.
authors.affiliation: Department of Radiopharmacy, Tohoku Pharmaceutical University, 4-4-1, Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan. n-sato@tohoku-pharm.ac.jp
authors.forename: Naoko
authors.initials: N
authors.lastname: Sato
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ohtake
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kohno
authors.forename: Shinya
authors.initials: S
authors.lastname: Abe
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Ohkubo
mesh_terms: D000818:Animals; D002364:Caseins / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004151:Dipeptides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020536:Enzyme Activators / Q000378:metabolism / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D011073:Polyamines / Q000378:metabolism / Q000494:pharmacology*; D011108:Polymers / Q000378:metabolism*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D011503:Transglutaminases / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002364:Caseins; D003432:Cross-Linking Reagents; D004151:Dipeptides; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; C002087:N,N-dimethylcasein; D011073:Polyamines; D011108:Polymers; C001299:epsilon-(gamma-glutamyl)-lysine; D013096:Spermine; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins; D013095:Spermidine; D011700:Putrescine