Validation of the multiplex kit genRESMPX-2 for forensic casework analysis.
Validation studies were carried out using the commercially available PCR multiplex system genRESMPX-2. In addition to amelogenin, this system comprises the complete set of eight STR systems which are components of the German DNA database established in 1998 by the Federal Criminal Office of Germany (BKA). The minimum amount of template DNA which gave a complete DNA pattern ranged between 100 pg and 200 pg. Mixed samples could clearly be assigned from ratios between 1:5 (ACTBP2) and 1:20 (VWA, FGA). Experimental investigations with different forensic materials, environmental studies, reproducibility and precision data as well as practical casework analysis revealed that the genRESMPX-2 kit can be regarded as a sensitive, reliable and robust multiplex system even in the case of samples containing limited amounts or degraded DNA.
Junge
A
A
Institute of Legal Medicine, Rheinische Friedrich-Wilhelms-University Bonn, Stiftsplatz 12, 53111, Bonn, Germany. a.junge@uni-bonn.de
Lederer
T
T
Braunschweiger
G
G
Madea
B
B
D000818:Animals; D004247:DNA / Q000097:blood / Q000737:chemistry; D016172:DNA Fingerprinting / Q000379:methods*; D030541:Databases, Genetic; D005554:Forensic Medicine / Q000295:instrumentation / Q000379:methods*; D005858:Germany; D006801:Humans; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D013045:Species Specificity; D020080:Tandem Repeat Sequences
D011933:Reagent Kits, Diagnostic; D004247:DNA