title: Feline foamy virus genome and replication strategy.
abstract: Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus.
authors.affiliation: Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Roy
authors.forename: Wolfram
authors.initials: W
authors.lastname: Rudolph
authors.forename: Thomas
authors.initials: T
authors.lastname: Juretzek
authors.forename: Kathleen
authors.initials: K
authors.lastname: Gärtner
authors.forename: Michael
authors.initials: M
authors.lastname: Bock
authors.forename: Ottmar
authors.initials: O
authors.lastname: Herchenröder
authors.forename: Dirk
authors.initials: D
authors.lastname: Lindemann
authors.forename: Martin
authors.initials: M
authors.lastname: Heinkelein
authors.forename: Axel
authors.initials: A
authors.lastname: Rethwilm
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016092:Spumavirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014162:Transfection; D014771:Virion / Q000378:metabolism / Q000472:pathogenicity; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral