title: Specific oligobodies against human brain acetylcholinesterase.
abstract: In order to develop the specific oligobodies against human brain acetylcholinesterase (AChE) and distinguishes between human erythrocyte and brain AChEs, we applied the strategy of 'target switching' to obtain the specific polyclonal and monoclonal oligobodies. The specificity between human brain AChE and other ChEs was identified by Western blotting, dot blotting and enzyme protein binding assay (EPBA). The results showed that the oligobodies against the human brain AChE specifically immunoreacted with the human brain AChE and Torpedo AChE, not showing significant binding to AChE from human erythrocyte and butyrylcholinesterase (BChE) from human serum.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijin 100850, China.
authors.forename: Xing Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Yu Sheng
authors.initials: YS
authors.lastname: Shi
authors.forename: Jun Hua
authors.initials: JH
authors.lastname: Wu
authors.forename: Ning Sheng
authors.initials: NS
authors.lastname: Shao
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Man Ji
authors.initials: MJ
authors.lastname: Sun
mesh_terms: D000110:Acetylcholinesterase / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002802:Cholinesterases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D011869:Radioligand Assay; D014101:Torpedo
chemical_list: D000906:Antibodies; D019151:Peptide Library; D010455:Peptides; D000110:Acetylcholinesterase; D002802:Cholinesterases