Preparation and modification of N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride nanoparticle as a protein carrier.
N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) is water-soluble derivative of chitosan (CS), synthesized by the reaction between glycidyl-trimethyl-ammonium chloride and CS. HTCC nanoparticles have been formed based on ionic gelation process of HTCC and sodium tripolyphosphate (TPP). Bovine serum albumin (BSA), as a model protein drug, was incorporated into the HTCC nanoparticles. HTCC nanoparticles were 110-180 nm in size, and their encapsulation efficiency was up to 90%. In vitro release studies showed a burst effect and a slow and continuous release followed. Encapsulation efficiency was obviously increased with increase of initial BSA concentration. Increasing TPP concentration from 0.5 to 0.7 mg/ml promoted encapsulation efficiency from 46.7% to 90%, and delayed release. As for modified HTCC nanoparticles, adding polyethylene glycol (PEG) or sodium alginate obviously decreased the burst effect of BSA from 42% to 18%. Encapsulation efficiency was significantly reduced from 47.6% to 2% with increase of PEG from 1.0 to 20.0 mg/ml. Encapsulation efficiency was increased from 14.5% to 25.4% with increase of alginate from 0.3 to 1.0 mg/ml.
Xu
Yongmei
Y
Department of Environmental Science, Wuhan University, Wuhan, Hubei 430072, China.
Du
Yumin
Y
Huang
Ronghua
R
Gao
Leping
L
D000327:Adsorption; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D008422:Materials Testing; D008968:Molecular Conformation; D009038:Motion; D043942:Nanotubes; D014677:Pharmaceutical Vehicles / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; C487756:N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride; D014677:Pharmaceutical Vehicles; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D002686:Chitin; D012710:Serum Albumin, Bovine