title: Post-transcriptional control of human maxiK potassium channel activity and acute oxygen sensitivity by chronic hypoxia.
abstract: Various cardiorespiratory diseases (e.g. congestive heart failure, emphysema) result in systemic hypoxia and patients consequently demonstrate adaptive cellular responses which predispose them to conditions such as pulmonary hypertension and stroke. Central to many affected excitable tissues is activity of large conductance, Ca2+-activated K+ (maxiK) channels. We have studied maxiK channel activity in HEK293 cells stably co-expressing the most widely distributed of the human alpha- and beta-subunits that constitute these channel following maneuvers which mimic severe hypoxia. At all [Ca2+]i, chronic hypoxia (approximately 18 mm Hg, 72 h) increased K+ current density, most markedly at physiological [Ca2+]i K+ currents in cells cultured in normoxia showed a [Ca2+]i-dependent sensitivity to acute hypoxic inhibition ( approximately 25 mm Hg, 3 min). However, chronic hypoxia dramatically changed the Ca2+ sensitivity of this acute hypoxic inhibitory profile such that low [Ca2+]i could sustain an acute hypoxic inhibitory response. Chronic hypoxia caused no change in alpha-subunit immunoreactivity with Western blotting but evoked a 3-fold increase in beta-subunit expression. These observations were fully supported by immunocytochemistry, which also suggested that chronic hypoxia augmented alpha/beta-subunit co-localization at the plasma membrane. Using a novel nuclear run-on assay and RNase protection we found that chronic hypoxia did not alter mRNA production rates or steady-state levels, which suggests that this important environmental cue modulates maxiK channel function via post-transcriptional mechanisms.
authors.affiliation: School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Hartness
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Brazier
authors.forename: Chris
authors.initials: C
authors.lastname: Peers
authors.forename: Alan N
authors.initials: AN
authors.lastname: Bateson
authors.forename: Michael L J
authors.initials: ML
authors.lastname: Ashford
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kemp
mesh_terms: D000222:Adaptation, Physiological; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002908:Chronic Disease; D004594:Electrophysiology; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D010100:Oxygen / Q000494:pharmacology*; D024681:Potassium Channels, Calcium-Activated / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D021122:Protein Subunits / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection
chemical_list: C494841:KCNMA1 protein, human; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D021122:Protein Subunits; D012333:RNA, Messenger; D010100:Oxygen; D002118:Calcium