Effect of transitory ischemia on liver lysosomal apparatus in rats with different resistance to hypoxia.
We studied the state of lysosomal apparatus and pro- and antioxidant activity in the liver of rats with different resistance to hypoxia during postischemic recovery. Under normal conditions the lysosomal apparatus did not differ in highly and low resistant animals. During ischemia and reperfusion the damage to hepatic lysosomal membranes in rats highly resistant to hypoxia was less pronounced than in low resistant animals. These differences also concerned labilization of lysosomes during exposure to damaging factors (hypotonia and Triton X-100). The rats highly resistant to hypoxia differed from low resistant animals by higher stability of lysosomal membranes, lower prooxidant activity (malonic dialdehyde content), and higher tissue concentration of alpha-tocopherol during reperfusion.
Grek
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OR
Department of Pharmacology, Central Research Laboratory, Novosibirsk State Medical Academy. nikagre@mail.cis.ru
Pupyshev
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AB
Tikhonova
E V
EV
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000860:Hypoxia; D007425:Intracellular Membranes / Q000378:metabolism; D007511:Ischemia; D008099:Liver / Q000473:pathology*; D008247:Lysosomes / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury; D012260:Ribonucleases / Q000378:metabolism; D013997:Time Factors; D024502:alpha-Tocopherol / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D008315:Malondialdehyde; D017830:Octoxynol; D012260:Ribonucleases; D000135:Acid Phosphatase; D001616:beta-Galactosidase; D024502:alpha-Tocopherol