Submersed culture production of extracellular wax esters by the marine bacterium Fundibacter jadensis.
Fundibacter jadensis strain T9, a marine gram-negative bacterium, was isolated from the intertidal sediment of the German North Sea coast by our group. The cells were able to produce considerable amounts of extracellular wax esters when cultivated with n-alkanes (hexadecane or tetradecane) as a carbon source. The dependence of wax ester production and the composition of the purified wax on different culture conditions (C:N:P ratio and dissolved oxygen tension) were tested. Our results show that wax ester production was not directly growth-linked. The C:N:P ratio had no significant influence on the yield of alkane-free purified wax. The dissolved oxygen tension affected the produced amount of the alkane-free purified wax and the composition of the purified wax; when lower than 2% it decreased the yield of purified wax and led to an altered wax ester composition. Tetradecane as a carbon source enhanced the spectrum of the wax ester composition.
Bredemeier
R
R
Institut für Chemie und Biologie des Meeres, CvO Universität Oldenburg, PO Box 2503, D-26111 Oldenburg, Germany.
Hulsch
R
R
Metzger
J O
JO
Berthe-Corti
L
L
D000473:Alkanes / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002855:Chromatography, Thin Layer; D009659:North Sea; D044130:Oceanospirillaceae / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D014885:Waxes / Q000737:chemistry / Q000378:metabolism*
D000473:Alkanes; D014885:Waxes; D010100:Oxygen