[Study on preparation of a wound healing agent: fibronectin].
In search of the optimal preparation method for large-scale purification of human plasma fibronectin, we adopted affinity chromatography with gelatin and the Sepharose 4B activated with cyanogen bromide to purify fibronectin from type "C" plasma of healthy males, and scanned the best method under the conditions of different amount of plasma loading and different residence time in column. In a given column volume of gelatin, the absorbent was related with the plasma residence time in column and the total amount of plasma loaded. As a result, the optimal loading amount of plasma is 150 ml, and the residence time is 20 minutes. The preparation method, herein, has been proved to require small amount of plasma and yield large amount of fibronectin.
Ren
Guowei
G
Traumatic Institute, Guangzhou Red Cross Hospital, Fourth Affiliated Hospital, Jinan University, Guangzhou 510220.
Huang
Xuefang
X
Dai
Libing
L
Ye
Huizhen
H
Liang
Peihong
P
D002846:Chromatography, Affinity / Q000379:methods*; D003488:Cyanogen Bromide / Q000737:chemistry; D005353:Fibronectins / Q000097:blood / Q000302:isolation & purification*; D005780:Gelatin; D006801:Humans; D008297:Male; D012685:Sepharose / Q000737:chemistry; D014945:Wound Healing
D005353:Fibronectins; D005780:Gelatin; D012685:Sepharose; D003488:Cyanogen Bromide