title: Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues.
abstract: This study evaluates the cross-reactivity of seven anti-human and one anti-bovine antibodies in formalin-fixed, paraffin-embedded tissue samples of liver and mesenteric lymph nodes of 13 striped dolphins (Stenella coeruleoalba). Four antibodies (CD3, IgG, lysozyme and S100 protein) reacted with striped dolphin lymph nodes in a similar pattern to that observed in the species of origin. The anti-human MHC class II mAb reacted strongly with macrophages and dendritic-like cells of striped dolphins, whereas a small number of lymphocytes were labelled with this antibody. These antibodies were used to study the immunophenotype of the inflammatory infiltrated in non-specific chronic reactive hepatitis (eight cases) and chronic parasite cholangitis (two cases) and normal liver (three cases) of striped dolphins. Non-specific chronic reactive hepatitis was composed of inflammatory infiltration of CD3+ T lymphocytes and IgG+ plasma cells in portal spaces and hepatic sinusoids. Lymphonodular aggregates observed in chronic parasitic cholangitis showed a cellular distribution similar to that found in lymph node cortex, including the presence of S100+ and MHC class II+ dendritic-like cells in lymphoid follicles and interfollicular areas. This result suggests that those inflammatory infiltrates are highly organised to enhance antigen presentation to B and T cells.
authors.affiliation: Departamento de Morfología, Facultad de Veterinaria de Las Palmas de Gran Canaria, Canaria, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Jaber
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: P
authors.initials: P
authors.lastname: Herráez
authors.forename: A
authors.initials: A
authors.lastname: Espinosa de los Monteros
authors.forename: G A
authors.initials: GA
authors.lastname: Ramírez
authors.forename: P M
authors.initials: PM
authors.lastname: García
authors.forename: T
authors.initials: T
authors.lastname: Fernández
authors.forename: M
authors.initials: M
authors.lastname: Arbelo
authors.forename: J
authors.initials: J
authors.lastname: Pérez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D004289:Dolphins / Q000276:immunology*; D005260:Female; D006520:Hepatitis, Animal / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008099:Liver / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D009113:Muramidase / Q000276:immunology; D016612:Paraffin Embedding / Q000662:veterinary; D009418:S100 Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D009418:S100 Proteins; D009113:Muramidase