Direct effects of cocaine-amphetamine-regulated transcript (CART) on pituitary hormone release in pituitary cell culture.
OBJECTIVE
Cocaine- and amphetamine-regulated transcript (CART) is widely expressed in the rat brain, especially in the hypothalamic nuclei and in the anterior pituitary. The aim of this study was to evaluate the effects of CART on pituitary hormone release in pituitary cell culture.


METHODS
The pituitary hormone release from pituitary cell culture after CART administration was investigated. Concentrations of LH, FSH, PRL, TSH and GH were measured with RIA methods.


RESULTS
CART in all doses (1 nMol, 10 nMol, 100 nMol) stimulated prolactin (PRL) release and inhibited TSH release. CART administration caused a dose dependent decrease in LH release. CART did not change GH release from cultured pituitary cells.


CONCLUSIONS
CART may affect directly pituitary hormones release in the cell culture.
Baranowska
Boguslawa
B
Neuroendocrinology Dept. Medical Centre of Postgraduate Education Warsaw, Poland. zncmkp@polbox.com
Woliñska-Witort
Ewa
E
Chmielowska
Magdalena
M
Martyñska
Lidia
L
Baranowska-Bik
Agnieszka
A
D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011921:Rats, Inbred WKY; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D010907:Pituitary Hormones; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone