title: SRC-1 localisation in lumbosacral spinal cord of male and female Wistar rats.
abstract: Nuclear receptor co-activators play an important role in enhancing transcriptional activity of steroid hormone receptors, however there is currently little information concerning their distribution within the spinal cord. In this study, the distribution of steroid receptor co-activator-1 (SRC-1) was examined with immunocytochemistry, in the lumbosacral cord of Wistar rats of both sexes. In all rats, regardless of sex, SRC-1 was predominant in neurons of the superficial laminae of the dorsal horn and within motorneurons of lamina IX. Sexually dimorphic nuclei exhibited robust SRC-1 immunoreactivity in young rats, including orchidectomised animals, but this appeared to decline in aged rats. Dorsal horn labelling appeared similarly reduced suggesting a possible age-related down-regulation of the transcription mediated by steroid receptors in some spinal neurons.
authors.affiliation: Cardiff School of Biosciences, Biomedical Sciences Building, Cardiff, UK. ransonrn@cardiff.ac.uk
authors.forename: Richard N
authors.initials: RN
authors.lastname: Ranson
authors.forename: Robert M
authors.initials: RM
authors.lastname: Santer
authors.forename: Alan H D
authors.initials: AH
authors.lastname: Watson
mesh_terms: D000375:Aging; D000818:Animals; D005260:Female; D051548:Histone Acetyltransferases; D007150:Immunohistochemistry; D008161:Lumbosacral Region / Q000033:anatomy & histology*; D008297:Male; D009474:Neurons / Q000378:metabolism; D056920:Nuclear Receptor Coactivator 1; D009919:Orchiectomy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism*; D012727:Sex Characteristics; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D051548:Histone Acetyltransferases; D056920:Nuclear Receptor Coactivator 1