Splice variant in TCRzeta links T cell receptor signaling to a G-protein-related signaling pathway.
The T cell receptor zeta chain is required for efficient receptor expression and contributes to T cell receptor-mediated activation of ZAP-70 and PLC-gamma1 as well as other signaling functions. A splice variant of zeta has been described which contains a 3bp insert coding for a glutamine in the cytoplasmic domain. The variant, here designated zeta-Q, is abundant, comprising 20-50% of zeta transcripts in humans, and production of the two isoforms is conserved among distantly related vertebrate species. Analysis of the peptide region in which the insert occurs reveals an unexpected homology with G-protein gamma chains. Transfection studies suggest that disruption in the alignment of three conserved prolines by the insertion of an extra glutamine impairs TCR-mediated PLC activation. Experiments with human lymphocytes suggest that zeta-Q message undergoes upregulation following cellular activation. Our data suggest that regulation of the relative levels of these two transcripts is related to an ancient mechanism which functions to raise the number of receptors required to produce cellular activation during the course of prolonged cellular stimulation, perhaps through a G-protein-related pathway.
Atkinson
T Prescott
TP
Department of Pediatrics, University of Alabama at Birmingham, 35294, USA. patkinso@uab.edu
Hall
Craig G
CG
Goldsmith
Jeff
J
Kirkham
Perry M
PM
D017398:Alternative Splicing; D000818:Animals; D017252:CD3 Complex / Q000096:biosynthesis; D002417:Cattle; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008958:Models, Molecular; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D010738:Type C Phospholipases / Q000378:metabolism; D015854:Up-Regulation
D017252:CD3 Complex; D018076:DNA, Complementary; D008565:Membrane Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; C054979:antigen T cell receptor, zeta chain; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins