4-Guanidino-2-pyrimidinone nucleobases: synthesis and hybridization properties.
N-Alkylated 4-guanidino-2-pyrimidinone-containing nucleosides, in which the guanidine group mimics the double hydrogen bond donor pattern of protonated cytosine, were introduced in polypyrimidine sequences to explore their triple-helix forming capabilites. UV and CD melting experiments showed that strands containing these base analogues did not form triplex complexes.
Robles
J
J
Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain. jrobles@qo.ub.es
Grandas
A
A
Pedroso
E
E
D006146:Guanidines; D006860:Hydrogen Bonding; D007202:Indicators and Reagents; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011743:Pyrimidines
D006146:Guanidines; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011743:Pyrimidines