Synthesis and recognition by DNA polymerases of a reactive nucleoside for DNA diversification.
The synthesis of 1-(2-deoxy-beta-D-erythro-pentofuranosyl)imidazole-4-hydrazide having the features of an ambigous base is reported. The recognition of the analogue by DNA polymerases as an incoming triphosphate as well as a template base was investigated. The mutagenic properties was evaluated by PCR. The potential of this new monomer for DNA diversification is illustrated by the reactivity of the nucleobase towards various aldehydes.
Pochet
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S
Institut Pasteur, Unité de Chimie Organique (URA CNRS 2128), Paris Cedex, France. spochet@pasteur.fr
Dugué
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L
Strobel
H
H
D004247:DNA / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D014644:Genetic Variation; D007093:Imidazoles; D007700:Kinetics; D016296:Mutagenesis; D009705:Nucleosides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity
D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D007093:Imidazoles; D009705:Nucleosides; C029603:deoxyguanosine triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C026600:2'-deoxyadenosine triphosphate