Inhibitory effect of kaurane type diterpenoids from Acanthopanax koreanum on TNF-alpha secretion from trypsin-stimulated HMC-1 cells.
Five known kaurane type diterpenoids, 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1), 16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16alpha-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity (IC50 value, 16.2 uM) on TNF-alpha secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.
Cai
Xing Fu
XF
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Shen
Guanghai
G
Dat
Nguyen Tien
NT
Kang
Ok Hwa
OH
Lee
Young Mi
YM
Lee
Jung Joon
JJ
Kim
Young Ho
YH
D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D028001:Eleutherococcus / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D007723:Korea; D007946:Leukemia, Mast-Cell / Q000139:chemically induced / Q000378:metabolism; D047311:Luteolin; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D014357:Trypsin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism*
C487399:16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid; D045786:Diterpenes, Kaurane; D005419:Flavonoids; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D014357:Trypsin; D047311:Luteolin; D000432:Methanol