title: Response of melanoma tumor phospholipid metabolism to chloroethyle nitrosourea: a high resolution proton NMR spectroscopy study.
abstract: Phospholipid metabolism is tightly involved in tumor growth regulation and tumor cell survival. The response of phospholipid metabolism to chloroethyle nitrosourea treatment is investigated in a murine B16 melanoma model. Measurements of phospholipid derivatives are performed on intact tumor tissue samples using one- and two-dimensional proton NMR spectroscopy. During the tumor growth inhibition phase under treatment, tumors overexpress phosphocholine, phosphoethanolamine, glycerophosphocholine and glycerophosphoethanolamine, whereas phosphatidylcholine and phosphatidylethanolamine levels are maintained to control levels. During re-growth, which remained quantitatively much below control growth, chloroethyle nitrosourea-treated melanoma tumors overexpress phosphocholine and phosphoethanolamine only. In treated melanoma, phosphatidylcholine levels show an inverse relationship with tumor growth rates. In conclusion, chloroethyle nitrosourea-treated melanoma tumors maintain their phosphatidylcholine levels and exhibit transformed phospholipid metabolism phenotype, by mechanisms that could participate in tumor cell survival.
authors.affiliation: UMR 484, rue Montalembert, 63005 Clermont-Ferrand, France. morvan@inserm484.u-clermont1.fr
authors.forename: Daniel
authors.initials: D
authors.lastname: Morvan
authors.forename: Aïcha
authors.initials: A
authors.lastname: Demidem
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Madelmont
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002470:Cell Survival; D004983:Ethanolamines / Q000032:analysis; D005997:Glycerylphosphorylcholine / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009607:Nitrosourea Compounds / Q000627:therapeutic use*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000378:metabolism*; D010767:Phosphorylcholine / Q000032:analysis
chemical_list: D000970:Antineoplastic Agents; D004983:Ethanolamines; D009607:Nitrosourea Compounds; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010767:Phosphorylcholine; C002449:glycerophosphoethanolamine; D005997:Glycerylphosphorylcholine; C005448:phosphorylethanolamine; C051263:N'-(2-chloroethyl)-N-(2-(methylsulfonyl)ethyl)-N'-nitrosourea