Smooth muscle cell outgrowth from coronary atherectomy specimens in vitro is associated with less time to restenosis and expression of a key Transcription factor KLF5/BTEB2.
Atherectomy specimens offer an opportunity to study the biology of coronary artery lesions. We cultured smooth muscle cells (SMCs) from specimens obtained from 24 patients with coronary restenosis after angioplasty to study the relationship between activity of SMCs (in vitro outgrowth) and the time course of restenosis. We also examined expression of a Kruppel-like zinc-finger transcription factor 5 (KLF; also known as BTEB2 and IKLF), which is markedly induced in activated SMCs, in the same specimens. SMC outgrowth was observed in 9 of 24 specimens (37.5%). Restenosis occurred sooner (p < 0.01) in patients whose specimens showed outgrowth compared to those whose specimens showed no outgrowth. Immunostaining for KLF5 was more common in specimens with outgrowth (89 vs. 20%, p < 0.01). These data suggest that the number of activated SMCs in lesions may determine in vitro outgrowth and also affect the time to restenosis.
Sakamoto
Hironosuke
H
Department of General Medicine, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. sakamoth@med.gunma-u.ac.jp
Sakamaki
Tetsuo
T
Kanda
Tsugiyasu
T
Hoshino
Yo-ichi
Y
Sawada
Yoshie
Y
Sato
Mahito
M
Sato
Hiroko
H
Oyama
Yuko
Y
Nakano
Akihiko
A
Takase
Shin-ichi
S
Hasegawa
Akira
A
Nagai
Ryozo
R
Kurabayashi
Masahiko
M
D000368:Aged; D017225:Atherectomy, Coronary; D002478:Cells, Cultured; D023903:Coronary Restenosis / Q000453:epidemiology / Q000378:metabolism* / Q000601:surgery*; D003331:Coronary Vessels / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology*; D012307:Risk Factors; D013223:Statistics as Topic; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis*
C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors; D015534:Trans-Activators