Activation of CEA-CAM-1-mediated cell adhesion via CD98: involvement of PKCdelta.
CD98 is a multifunctional protein involved in amino acid transport and regulation of integrin-mediated cell adhesion. Herein, we demonstrated that CD98 stimulation by anti-CD98 antibodies induced CEA-CAM-1-mediated cell adhesion in BaF3 cells expressing CEA-CAM-1, and suggest that this might be responsible for compact clumping of F9 embryonic carcinoma cells by CD98 stimulation. CEA-CAM-1 was co-immunoprecipitated by anti-CD98 antibody. CD98 stimulation induced the translocation of cytoplasmic protein kinase Cdelta (PKCdelta) to the cell adhesion sites, and rottlerin that inhibited the PKCdelta translocation abolished the cell aggregation without affecting integrin activation. The results suggested that CD98 stimulation could activate CEA-CAM-1-mediated cell adhesion independently of integrins.
Kakugawa
Kiyokazu
K
Department of Biophysics, Graduate School of Science, Kyoto University, 606-8502 Kyoto, Japan.
Hattori
Masakazu
M
Beauchemin
Nicole
N
Minato
Nagahiro
N
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD; D002118:Calcium / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D027261:Fusion Regulatory Protein-1 / Q000502:physiology*; D006023:Glycoproteins; D051379:Mice; D011493:Protein Kinase C / Q000502:physiology*; D051745:Protein Kinase C-delta; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C087701:Ceacam2 protein, mouse; D015815:Cell Adhesion Molecules; D027261:Fusion Regulatory Protein-1; D006023:Glycoproteins; D011994:Recombinant Proteins; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D000251:Adenosine Triphosphatases; D002118:Calcium