Phosphono peptide nucleic acids with a constrained hydroxyproline-based backbone.
DNA mimics representing negatively charged analogues of peptide nucleic acids (PNAs), particularly hetero-oligomers constructed from alternating phosphono-PNA residues (pPNA) and monomers on the base of trans-4-hydroxy-L-proline (HypNA) as well as mimics composed of phosphono-HypNA monomers (pHypNA) were tested in a set of in vitro and in vivo assays, and they demonstrated a high potential for the use in nucleic acid based diagnostic, isolation of nucleic acids and antisense experiments.
Efimov
Vladimir A
VA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. eva@mail.ibch.ru
Klykov
Valeryi N
VN
Chakhmakhcheva
Oksana G
OG
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D006909:Hydroxyproline; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D063065:Organophosphonates; D020135:Peptide Nucleic Acids; D012313:RNA; D004247:DNA; D006909:Hydroxyproline