Efficiency of the hybrid capture 2 HPV DNA test in cervical cancer screening. A study by the French Society of Clinical Cytology.
The aim of this study was to determine the efficiency of the Hybrid Capture 2 (HC2; Digene, Gaithersburg, MD) human papillomavirus (HPV) assay for the detection of cervical neoplasia. Of the 1,785 patients recruited, 462 (25.88%) were referred for colposcopy owing to previously detected cytologic abnormalities, and 1,323 (74.12%) were voluntary candidates for screening. For all patients, a Papanicolaou smear and a monolayer smear (ThinPrep, Cytyc, Boxborough, MA) were done. HPV DNA was detected on the residual liquid-based material. False-positive results were observed in 111 cases and comprised 34 cross-reactions (1.90%) and 77 false-positive cases (4.31%) owing to a contiguous strong chemiluminescence signal. Interestingly, all these samples had a relative light units value of 1 to 3 and were contiguous to a sample with a very high HPV DNA load. The final results showed that high-risk and low-risk HPV DNA were detected in 480 samples (26.89%) and 135 samples (7.56%), respectively. Although HC2 can be considered a reliable and sensitive test for HPV DNA detection, we do not advocate its use for large-scale screening for cervical neoplasia.
de Cremoux
Patricia
P
Department of Tumor Biology, Institut Curie, Paris, France.
Coste
Joël
J
Sastre-Garau
Xavier
X
Thioux
Martine
M
Bouillac
Christelle
C
Labbé
Sylvain
S
Cartier
Isabelle
I
Ziol
Marianne
M
Dosda
Anne
A
Le Galès
Catherine
C
Molinié
Vincent
V
Vacher-Lavenu
Marie-Cécile
MC
Cochand-Priollet
Béatrix
B
Vielh
Philippe
P
Magdelénat
Henri
H
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D003951:Diagnostic Errors; D005260:Female; D005602:France; D006801:Humans; D008403:Mass Screening / Q000191:economics / Q000379:methods*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012958:Societies, Scientific; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000517:prevention & control; D014626:Vaginal Smears
D004279:DNA, Viral