[Protective effects of tanshinone IIA on injured primary cultured rat hepatocytes induced by CCl4].
OBJECTIVE
To investigate the effect of tanshinone IIA on carbon tetrachloridlde (CCl4) intoxicated primary cultured rat hepatocytes.


METHODS
The rat hepatocytes were separated by perfusion with collagenase solution. Tanshinone IIA was added to the cultured medium after incubating the rat hepatocytes for 36 hours. At the same time, CCl4 was added to induce hepatocytes injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined respectively.


RESULTS
Tanshinone IIA significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels


CONCLUSIONS
Tanshinone IIA protected effectively the primary cultured rat hepatocytes against CCl4-induced injury.
Liu
Yongzhong
Y
Center Hospital of Xi'an, Xi'an 710003.
Wang
Xiaodong
X
Liu
Yonggang
Y
D045784:Abietanes; D000818:Animals; D002251:Carbon Tetrachloride; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000378:metabolism; D008297:Male; D010616:Phenanthrenes / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry
D045784:Abietanes; D010616:Phenanthrenes; D020011:Protective Agents; C021751:tanshinone; D002251:Carbon Tetrachloride