Photoregulation of deacylation rate of acyl trypsin derived from photoresponsive inverse substrate.
The acyl trypsin was prepared by use of an inverse substrate, which is comprise of a photoresponsive 4-phenylazobenzoyl moiety. The acyl group in acyl trypsin has been shown to isomerize from trans-form (4t-trypsin) to cis-form (4c-trypsin)/from cis-form to trans-form by irradiation of UV-vis light. The deacylation rate of the cis-form (4c-trypsin) has been shown to be 18.6 times faster than that of the trans-form (4t-trypsin).
Sekizaki
Haruo
H
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. sekizaki@hoku-iryo-u.ac.jp
Kumagai
Asako
A
Itoh
Kunihiko
K
Toyota
Eiko
E
Horita
Kiyoshi
K
Noguchi
Yukari
Y
Tanizawa
Kazutaka
K
D003640:Dealkylation; D006868:Hydrolysis; D007700:Kinetics; D010777:Photochemistry; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000737:chemistry*; D014466:Ultraviolet Rays
D014357:Trypsin