title: Synthesis and energy transfer efficiency of FRET terminators derived from different linkers.
abstract: A number of different energy transfer dye labeled-cassettes were synthesized using aminoacid based trifunctional linkers and coupled to the propargylamino-substituted dideoxynucleoside-5'-triphosphates (ddNTPs). These terminators were evaluated for their energy transfer efficiency and DNA sequencing potential using thermostable DNA polymerase.
authors.affiliation: Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA. shiv.kumar@amersham.com
authors.forename: Shiv
authors.initials: S
authors.lastname: Kumar
authors.forename: Satyam
authors.initials: S
authors.lastname: Nampalli
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Finn
authors.forename: T
authors.initials: T
authors.lastname: Sudhakar Rao
authors.forename: Chung-Yuan
authors.initials: CY
authors.lastname: Chen
authors.forename: Parke K
authors.initials: PK
authors.lastname: Flick
authors.forename: Carl W
authors.initials: CW
authors.lastname: Fuller
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D013728:Terminator Regions, Genetic
chemical_list: D005456:Fluorescent Dyes; D004247:DNA; D004259:DNA-Directed DNA Polymerase