New cell-cell adhesion inhibitors from Streptomyces sp. UMA-044.
Three compounds, NP25301 (1), NP25302 (2) and bohemamine (3), inhibitors of cell adhesion based on LFA-1/ICAM-1, were isolated from the cultured broth of the strain Streptomyces sp. UMA-044. New compounds 1 and 2 were identified as 2-(3'-carbamoylphenoxy)acrylic acid methyl ester and deoxybohemamine, respectively, based on spectroscopic analyses. Compounds 1 approximately 3 inhibited adhesion of HL-60 cells to CHO-ICAM-1 cells at IC50 values of 29.5 microg/ml for 1, 24.3 microg/ml for 2, and 27.2 microg/ml for 3.
Zhang
Qinglin
Q
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA.
Schrader
Kevin K
KK
ElSohly
Hala N
HN
Takamatsu
Satoshi
S
D000818:Animals; D001549:Benzamides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects*; D002934:Cinnamates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006224:Cricetinae; D005285:Fermentation; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects*; D011763:Pyrrolizidine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces
D001549:Benzamides; D002934:Cinnamates; C478486:NP 25301; C478487:NP 25302; D011763:Pyrrolizidine Alkaloids; D018799:Intercellular Adhesion Molecule-1; C025548:bohemamine