title: Proteome analysis of Salmonella enterica serovar Typhimurium fis mutant.
abstract: Salmonella enterica serovar Typhimurium is an enteric pathogen and a principal cause of gastroenteritis in humans. The factor-for-inversion stimulation protein (Fis) is known to play a pivotal role in the expression of Salmonella pathogenicity island (SPI)-1 genes in addition to various cellular processes such as recombination, replication, and transcription. In order to understand Fis function in pathogenicity of Salmonella, we performed two-dimensional gel electrophoresis and identified proteins whose expression pattern is affected by Fis using mass spectrometry. The results revealed various proteins that can be grouped according to their respective cellular functions. These groups include the genes involved in the metabolism of sugar, flagella synthesis, translation, and SPI expression. Changes in SPI expression suggest the possibility that regulation of genes in SPI-2 as well as SPI-1 is affected by Fis.
authors.affiliation: Department of Food Science and Technology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Yoon
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
authors.forename: Sangho
authors.initials: S
authors.lastname: Choi
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000096:biosynthesis; D035021:Factor For Inversion Stimulation Protein / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D044404:Genomic Islands / Q000235:genetics; D013058:Mass Spectrometry; D018832:Molecular Chaperones / Q000096:biosynthesis; D009154:Mutation; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D035021:Factor For Inversion Stimulation Protein; D018832:Molecular Chaperones; D020543:Proteome