title: Analysis of T-cell receptor Vbeta gene from infiltrating T cells in insulitis and myocarditis in encephalomyocarditis virus-infected BALB/C mice.
abstract: Encephalomyocarditis (EMC) virus induces insulin-dependent diabetes and myocarditis in several strains of mice. The T-cell receptor (TCR) Vbeta genes of infiltrating T cells in the pancreas and myocardium of BALB/C mice infected with EMC virus D-variant (EMC-D virus) were analyzed. Using a nested two-step polymerase chain reaction (PCR), TCR Vbeta cDNAs were cloned and sequenced. Two and four kinds of TCR Vbeta clones were obtained from T cells infiltrating into the pancreas and myocardium of BALB/C mice infected with EMC-D virus, respectively. The infiltrating lymphocytes in the diabetic mice expressed Vbeta 8.1, 8.2, and 8.3 genes predominantly. Previously, the use of Vbeta 8.2 has been reported in autoimmune diseases such as murine experimental allergic encephalomyelitis (EAE) and non-obese diabetic (NOD) mouse. This study suggests that mice infected with EMC virus are a useful animal model for autoimmune diseases such as insulin-dependent diabetes.
authors.affiliation: Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Kawagishi
authors.forename: Atsutaka
authors.initials: A
authors.lastname: Kubosaki
authors.forename: Natsumi
authors.initials: N
authors.lastname: Takeyama
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.forename: Keiichi
authors.initials: K
authors.lastname: Saeki
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D015551:Autoimmunity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000821:virology; D004686:Encephalomyocarditis virus / Q000276:immunology*; D019672:Genes, T-Cell Receptor / Q000235:genetics; D005951:Glucose Tolerance Test; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008957:Models, Genetic; D009205:Myocarditis / Q000276:immunology* / Q000821:virology*; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D001324:Autoantigens; D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012313:RNA