title: A simple and eficient method for high fidelity PCR cloning using antibody-neutralizing technology.
abstract: We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq.
authors.affiliation: Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Kitabayashi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nishiya
authors.forename: Muneharu
authors.initials: M
authors.lastname: Esaka
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry*; D003001:Cloning, Molecular / Q000379:methods*; D004259:DNA-Directed DNA Polymerase / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D009500:Neutralization Tests / Q000379:methods; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism; D013824:Thermus / Q000201:enzymology / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D019384:Nucleic Acid Synthesis Inhibitors; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase