[Experimental study on effect of platelet-rich plasma in repair of bone defect].
OBJECTIVE
To study the effect of platelet-rich plasma in the repair of bone defect.


METHODS
Segmental bone defects of 1 cm were created in the mid-upper part of bilateral radius of 24 New Zealand white rabbits. One side was randomly chosen as the experimental side, which was filled with artificial bone with platelet-rich plasma (PRP). The other side filled with artificial bone without PRP as the control. After 2, 4, 8 and 12 weeks of implantation, the gross, radiological, histological observations, and computer graphic analysis were performed to investigate the bone healing of the defect in both sides.


RESULTS
Two weeks after operation, new bone and fibrous tissue formation in both the experimental and the control sides were observed only in the areas adjacent to the cut ends of the host bone, but the amount of new tissue in the experimental side was much more than that in the control side. In the 4th and 8th weeks, the surface of the artificial bone was covered with a large amount of new bones, the artificial bone was bridged tightly with the host bone by callus in the experimental side, while new bone was limited mainly in the cut ends and was less mature in the control side. In the 12th weeks, bone defects were entirely healed in the experimental side, which were covered completely with cortical bone, while new bone formation was only observed in the ends of artificial bone and there were not continuous bone callus on the surface in the control side.


CONCLUSIONS
Artificial bone with PRP is effective in the repair of segmental bone defects, and PRP could improve the healing of bone defect.
Zhang
Chang-qing
CQ
Department of Orthopedics, Sixth People's Hospital Affiliated Shanghai Jiao Tong University, Shanghai, P. R. China 200233.
Yuan
Ting
T
Zeng
Bing-fang
BF
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001861:Bone Regeneration; D018786:Bone Substitutes; D002516:Ceramics; D005260:Female; D017102:Fracture Healing; D020007:Implants, Experimental; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011817:Rabbits; D011885:Radius Fractures / Q000601:surgery*; D011897:Random Allocation; D044967:Serum / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D018786:Bone Substitutes; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta