title: Viability of the antigen determines whether DNA or urocanic acid act as initiator molecules for UV-induced suppression of delayed-type hypersensitivity.
abstract: UV radiation suppresses the immune response, and UV-induced immune suppression contributes to UV-induced photocarcinogenesis. For UV-induced immune suppression to occur, electromagnetic energy (i.e. UV radiation) must be converted to a biological signal. Two photoreceptors have been identified in the skin that serves this purpose, epidermal DNA and trans-urocanic acid (UCA). Although compelling evidence exists to support a role for each pathway (UV-induced DNA damage or photoisomerization of UCA) in UV-induced immune suppression, it is not clear what determines which photoreceptor pathway is activated. To address this question, we injected UV-irradiated mice with a monoclonal antibody with specificity for cis-UCA or applied liposomes containing DNA repair enzymes to the skin of UV-irradiated mice. The effect that each had on UV-induced suppression of delayed-type hypersensitivity was measured. We asked whether the light source used (FS-40 sunlamps vs solar-simulated UV radiation) altered whichever pathway of immune suppression was activated. Different doses of UV radiation and the viability of the antigen were also considered. Neither the dose of UV nor the light source had any influence on determining which pathway was activated. Rather, we found that the viability of the antigen was the critical determinant. When live antigens were used, UV-induced immune suppression was blocked with monoclonal anti-cis-UCA but not with T4 endonuclease V-containing liposomes. The reverse was observed when formalin-fixed or killed antigens were used. Our findings indicate that antigen viability dictates which photoreceptor pathway predominates after UV exposure.
authors.affiliation: Department of Dermatology, College of Medicine, Gyeongsang National University, Kyungnam, Korea.
authors.forename: Tae-Hueng
authors.initials: TH
authors.lastname: Kim
authors.forename: Angus M
authors.initials: AM
authors.lastname: Moodycliffe
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Yarosh
authors.forename: Mary
authors.initials: M
authors.lastname: Norval
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Kripke
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Ullrich
mesh_terms: D000818:Animals; D004247:DNA / Q000502:physiology*; D005260:Female; D006968:Hypersensitivity, Delayed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000378:metabolism*
chemical_list: D004247:DNA; D014560:Urocanic Acid