title: Molecular confirmation of human alveolar echinococcosis in Poland.
abstract: Infections of humans with Echinococcus multilocularis, the causative agent of alveolar echinococcosis (AE), a zoonosis, have been described with increasing frequency in Poland since 1994. In the attempt to verify these reports, we analyzed specimens obtained from a representative group of Polish patients. Liver lesions in patients with AE that was diagnosed on the basis of results of histological and serological tests contained E. multilocularis DNA, as shown by the presence of specific microsatellite sequences and mitochondrial 12S rDNA. The same tests clearly distinguished between AE and cystic echinococcosis, which is caused by Echinococcus granulosus. These data are unequivocal proof that human infections with E. multilocularis occur in Poland.
authors.affiliation: Institute of Maritime and Tropical Medicine, Gdynia, Poland. pemyjak@immt.gdynia.pl
authors.forename: Przemysław
authors.initials: P
authors.lastname: Myjak
authors.forename: Waclaw
authors.initials: W
authors.lastname: Nahorski
authors.forename: Halina
authors.initials: H
authors.lastname: Pietkiewicz
authors.forename: Markus
authors.initials: M
authors.lastname: von Nickisch-Rosenegk
authors.forename: Juliusz
authors.initials: J
authors.lastname: Stolarczyk
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kacprzak
authors.forename: Iwona
authors.initials: I
authors.lastname: Felczak-Korzybska
authors.forename: Beata
authors.initials: B
authors.lastname: Szostakowska
authors.forename: Richard
authors.initials: R
authors.lastname: Lucius
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D018509:DNA, Helminth / Q000032:analysis; D004444:Echinococcosis, Hepatic / Q000175:diagnosis*; D004446:Echinococcus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D011044:Poland; D012698:Serologic Tests
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth