[Effects of tengcha flavonoids on scavenging oxygen free radicals and inhibiting lipid-peroxidation].
OBJECTIVE
To study the effects of Tengcha flavonoids (TCF) on scavenging oxygen free radicals and inhibiting lipid-peroxidation.


METHODS
The effects of TCF on scavenging superoxide anion radical induced by xanthine-xanthine oxidase, inhibiting lipid-peroxidation of heart, liver, brain tissue homogenate and mitochondria induced by Fe2+ -VitC, Fe2+ -H2O2, Fe-Cys were discussed by TBA spectrometric method.


RESULTS
TCF could significantly inhibit the superoxide anion radical, IC50 is 14.7 mg/L, and the lipid peroxidation of liver homogenate and mitochondria induced by auto-oxidation and induced-oxidation. The inhibition exhibited concentration-dependent manner.


CONCLUSIONS
It indicated that TCF could scavenge oxygen free radicals and was capable of prophylacticly antagonizing against the oxidative injury by superoxide anion.
He
Guixia
G
Hunan College of Traditional Chinese Medicine, Changsha 410007.
Du
Fanglu
F
Yang
Weili
W
Pei
Gang
G
Zhu
Yonghua
Y
D032421:Ampelopsis / Q000737:chemistry*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008933:Mitochondrial Swelling / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D005609:Free Radicals; D010100:Oxygen