Apoprotein(a) phenotypes and plasma lipoprotein(a) concentration in patients with diabetes mellitus.
OBJECTIVE
To determine whether apo(a) isoforms and plasma Lp(a) concentrations in association with some lipid parameters increase the relative risk for the development of atherosclerosis in patients with diabetes mellitus (IDDM and NIDDM).


METHODS
Apo (a) isoforms, Lp(a) and plasma lipids were determined in 40 IDDM and 65 NIDDM patients and in 182 healthy individuals. Apo(a) isoforms were separated by 3 to 15% gradient SDS-PAGE followed by immunoblotting.


RESULTS
Logistical analysis showed that: Lp(a) levels >30 mg/dL (RR = 0.25, p < 0.000001; RR = 0.18, p < 0.00002), HTA (RR = 0.212, p < 0.00001; RR = 0.30, p < 0.00001), LMW-S1 apo(a) isoform (RR = 6.86, p < 0.0131; RR = 7.04, p < 0.0057) play a significant role in aterogenecity in both groups of patients with DM (IDDM and NIDDM). The 6.50-fold increase in risk was found in NIDDM patients with high Lp(a) levels (>30 mg/dL) and plasma total/HDL cholesterol ratio (4.5-5.8).


CONCLUSIONS
Elevated Lp(a) levels, LMW S1 apo(a) isoform, HTA and combination of increased Lp(a) levels and total/HDL cholesterol ratio increase the risk for the development of atherosclerosis in patients with DM (IDDM and NIDDM).
Labudovic
Danica D
DD
Department of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Macedonia. dlabudovic@yahoo.com
Toseska
Katerina N
KN
Alabakovska
Sonja B
SB
B  Todorova
Bojana
B
D001054:Apolipoproteins A / Q000097:blood* / Q000235:genetics*; D001161:Arteriosclerosis / Q000097:blood / Q000150:complications / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications / Q000235:genetics*; D004198:Disease Susceptibility; D005260:Female; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood*; D008297:Male; D010641:Phenotype; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics
D001054:Apolipoproteins A; D017270:Lipoprotein(a); D020033:Protein Isoforms; D002784:Cholesterol