B7-H1 blockade augments adoptive T-cell immunotherapy for squamous cell carcinoma.
In this report, we demonstrate that B7-H1, a B7 family molecule implicated in tumor immune evasion, is constitutively expressed on 66% of freshly isolated squamous cell carcinomas of the head and neck (SCCHN). To define the potential impact of tumor-associated B7-H1 on immunotherapy, the B7-H1-negative mouse SCC line, SCCVII, was transfected to express B7-H1. Although all of the animals succumbed to B7-H1/SCCVII tumors even after adoptive T-cell immunotherapy, the infusion of B7-H1 blocking monoclonal antibody with activated T cells cured 60% of animals. These data support B7-H1 blockade as a new approach to enhance the efficacy of T-cell immunotherapy.
Strome
Scott E
SE
Department of Otolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. strome.scott@mayo.edu
Dong
Haidong
H
Tamura
Hideto
H
Voss
Stephen G
SG
Flies
Dallas B
DB
Tamada
Koji
K
Salomao
Diva
D
Cheville
John
J
Hirano
Fumiya
F
Lin
Wei
W
Kasperbauer
Jan L
JL
Ballman
Karla V
KV
Chen
Lieping
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D060890:B7-H1 Antigen; D001798:Blood Proteins; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; D001798:Blood Proteins; C423236:CD274 protein, human; C498919:Cd274 protein, mouse; D008562:Membrane Glycoproteins; D010455:Peptides