title: [Mutation of envelop protein V3 loop in HIV-1 epidemic in Liaoning province].
abstract: OBJECTIVE
  To study the characteristics of human immunodeficiency virus (HIV-1) V3 loop amino acid mutations among HIV-1 infected people in Liaoning province.
  
  
  METHODS
  The whole blood samples of the HIV carriers and AIDS patients were collected in Liaoning province, China and were extracted PBMC genome DNA. HIV-1 V3 and flanking region sequences were amplified by nest-polymerase chain reaction (nest-PCR) with env specific primers: ED5/ED12 and ED31/ED33. Products were sequenced directly and sequences were aligned, translated and analyzed.
  
  
  RESULTS
  In AIDS group, some amino acid mutations at specific position of V3 loop: S to R at position 11, H to S, T and N at position 13, A to V at position 19, F to Y at position 20, Q or D to N at position 25 and 29, were found and all common mutations were associated with T tropic/SI phenotype. The frequency of such amino acid mutations in specific positions was higher in AIDS group than that of the asymptomatic infection group (P < 0.05). In addition, we found some unusual tetramer compositions on the tip of V3 loop: GQGR, APGR and RPGA, GLGR, RPGA in addition to some rare mutations, such as: N to H at position 5 and H to S, F at position 34.
  
  
  CONCLUSIONS
  The amino acid mutations on the V3 loop of HIV-1 epidemic in Liaoning province were in agreement with the results of subtype B, but we observed some rare mutations and unusual tetramer compositions on the tip of V3 loop.
authors.affiliation: P3 Laboratory the 1st Affiliated Hospital China Medical University, Shenyang 110001, China.
authors.forename: Xiao-xu
authors.initials: XX
authors.lastname: Han
authors.forename: Hong
authors.initials: H
authors.lastname: Shang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhou
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Zi-ning
authors.initials: ZN
authors.lastname: Zhang
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Jiang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000821:virology; D000328:Adult; D000595:Amino Acid Sequence; D002648:Child; D002681:China / Q000453:epidemiology; D005260:Female; D015752:Genes, env / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D015699:HIV Envelope Protein gp120; D014759:Viral Envelope Proteins