Amplified-fragment length polymorphism as a complement to IS6110-based restriction fragment length polymorphism analysis for molecular typing of Mycobacterium tuberculosis.
The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.
Ruiz
Monserrat
M
Section of Microbiology, Hospital General Universitario de Elche, Elche (Alicante), Spain. micro_elx@gva.es
Rodríguez
Juan Carlos
JC
Rodríguez-Valera
Francisco
F
Royo
Gloria
G
D015373:Bacterial Typing Techniques; D004251:DNA Transposable Elements; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
D004251:DNA Transposable Elements