title: Acetylcholinesterase activity and molecular isoform distribution are altered after focal cerebral ischemia.
abstract: Biochemical changes in rat brain acetylcholinesterase (AChE) activity and molecular isoform distribution were studied using the middle cerebral artery occlusion model. After 24 h of permanent focal ischemia, AChE specific activity from infarcted cortex was found to decrease compared with contralateral cortex. Also a decrease in the minor monomeric (G1) form of AChE is response to ischemia was observed. Ischemia-induced AChE decrease was partially diminished by pre-treatment with dizocilpine (MK-801), a neuroprotective NMDA receptor blocker.
authors.affiliation: Instituto de Neurociencias, Universidad Miguel Hernández, San Juan de Alicante, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Sáez-Valero
authors.forename: Carmen
authors.initials: C
authors.lastname: González-García
authors.forename: Valentín
authors.initials: V
authors.lastname: Ceña
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D016291:Dizocilpine Maleate / Q000494:pharmacology / Q000627:therapeutic use; D007839:Functional Laterality; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D020033:Protein Isoforms / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D018696:Neuroprotective Agents; D020033:Protein Isoforms; D016291:Dizocilpine Maleate; D000110:Acetylcholinesterase