title: The introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco.
abstract: Phytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture.
authors.affiliation: Department of Botany, The University of Hong Kong, Pokfulam, Hong Kong, China. wkyip@hkucc.hku.hk
authors.forename: Wingkin
authors.initials: W
authors.lastname: Yip
authors.forename: Lingjian
authors.initials: L
authors.lastname: Wang
authors.forename: Chiwai
authors.initials: C
authors.lastname: Cheng
authors.forename: Wailan
authors.initials: W
authors.lastname: Wu
authors.forename: Shiucheung
authors.initials: S
authors.lastname: Lung
authors.forename: Boon-Leong
authors.initials: BL
authors.lastname: Lim
mesh_terms: D010832:6-Phytase / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005798:Genes, Bacterial; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development*
chemical_list: D017931:DNA Primers; D010832:6-Phytase