Influence of anticoagulants on the measurement of S100B protein in blood.
OBJECTIVE
Evaluate anticoagulants influence on plasma S100B levels.


METHODS
Blood were collected from 18 healthy adult subjects using: no anticoagulants, EDTA, heparin, and citrate. S100B levels were determined using LIA-mat assay.


RESULTS
Heparin plasma and citrate increased plasma S100B levels (p < 0.001), whereas EDTA had no effect (p = 0.24). Heparin plasma samples were highly (r2 = 0.97, p < 0.001), citrate samples were moderately (r2 = 0.49, p < 0.001), and EDTA samples were not (r2 = 0.22, p = 0.059) correlated with serum samples.


CONCLUSIONS
When anticoagulant is required, heparin plasma should be the primary choice for measurement of S100 B levels.
Tort
Adriano B L
AB
Departamento de Bioquímica, ICBS, UFRGS, Brazil.
Dietrich
Marcelo O
MO
Gonçalves
Carlos A
CA
Souza
Diogo O
DO
Portela
Luis V C
LV
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D016477:Artifacts; D019343:Citric Acid; D004492:Edetic Acid; D005260:Female; D006493:Heparin; D006801:Humans; D008297:Male; D009418:S100 Proteins / Q000097:blood*
D000925:Anticoagulants; D009418:S100 Proteins; D019343:Citric Acid; D006493:Heparin; D004492:Edetic Acid