title: Probucol decreases mevalonate pyrophosphate decarboxylase in the rat liver.
abstract: It is known that cholesterol biosynthesis in the liver is inhibited by probucol. This inhibition by probucol is caused at least in part by a decrease in 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase activity. In this study, we examined serum cholesterol and the change in the activity or protein level of mevalonate pyrophosphate decarboxylase (MPD), which is involved in cholesterol biosynthesis, in the livers of rats fed probucol. The results indicated that serum cholesterol, MPD activity and MPD protein were decreased by 70, 50 and 60% by probucol, respectively, as compared with those in rats fed normal chow. These data show for the first time that probucol decreases the level of an enzyme involved in cholesterol biosynthesis other than HMG-CoA reductase.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. mitihara@fupharm.fukuyama-u.ac.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Michihara
authors.forename: Makoto
authors.initials: M
authors.lastname: Sawamura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamori
authors.forename: Kenji
authors.initials: K
authors.lastname: Akasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D011341:Probucol / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D002784:Cholesterol; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase; D011341:Probucol