title: Isolation and characterization of phenol-catabolizing bacteria from a coking plant.
abstract: New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a Vmax of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.
authors.affiliation: Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Wael S
authors.initials: WS
authors.lastname: El-Sayed
authors.forename: Mohamed K
authors.initials: MK
authors.lastname: Ibrahim
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abu-Shady
authors.forename: Fawkia
authors.initials: F
authors.lastname: El-Beih
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohmura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saiki
authors.forename: Akikazu
authors.initials: A
authors.lastname: Ando
mesh_terms: D000332:Aerobiosis; D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D003077:Coke / Q000382:microbiology*; D007700:Kinetics; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000447:Aldehydes; D002396:Catechols; D003077:Coke; D010636:Phenols