title: Isolation and characterization of a maintenance DNA-methyltransferase gene from peach (Prunus persica [L.] Batsch): transcript localization in vegetative and reproductive meristems of triple buds.
abstract: A cDNA coding for a DNA (cytosine-5)-methyltransferase (METase) was isolated from peach (Prunus persica [L.] Batsch) and the corresponding gene designated as PpMETI. The latter encoded a predicted polypeptide of 1564 amino acid residues and harboured all the functional domains conserved in the maintenance METases group type I. PpMETI was a single copy in the cultivar Chiripa which was used as a model in the present study. Expression analyses revealed that PpMETI transcripts were more abundant in tissues with actively proliferating cells such as apical tips, uncurled leaves, elongating herbaceous stems, and small immature fruits. Peach plants bear bud clusters (triads or triple buds), consisting of two lateral and one central bud with floral and vegetative fates, respectively. PpMETI in situ hybridization was performed in triple buds during their entire developmental cycle. High and low levels of PpMETI transcript were related to burst and quiescence of vegetative growth, respectively. Message localization distinguished lateral from central buds during the meristem switch to the floral phase. In fact, the PpMETI message was abundant in the L1 layer of protruding domes, a morphological trait marking the beginning of floral transition. The PpMETI transcript was also monitored during organ flower formation. Altogether, these data suggest a relationship between DNA replication and PpMETI gene expression.
authors.affiliation: Institute of Plant Protection, Section of Bari, National Research Council of Italy (CNR), Via Amendola 165/A, I-70126 Bari, Italy. giannino@mlib.cnr.it
authors.forename: Donato
authors.initials: D
authors.lastname: Giannino
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mele
authors.forename: Radiana
authors.initials: R
authors.lastname: Cozza
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bruno
authors.forename: Giulio
authors.initials: G
authors.lastname: Testone
authors.forename: Carla
authors.initials: C
authors.lastname: Ticconi
authors.forename: Giovanna
authors.initials: G
authors.lastname: Frugis
authors.forename: Maria Beatrice
authors.initials: MB
authors.lastname: Bitonti
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Innocenti
authors.forename: Domenico
authors.initials: D
authors.lastname: Mariotti
mesh_terms: D000818:Animals; D001483:Base Sequence; D015254:DNA Modification Methylases / Q000235:genetics*; D017931:DNA Primers; D035264:Flowers / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006801:Humans; D018519:Meristem / Q000201:enzymology / Q000235:genetics*; D018520:Plant Shoots / Q000201:enzymology; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015254:DNA Modification Methylases