Human immunodeficiency virus type 1 RNA in breast-milk components.
We conducted the present study to determine which of the 4 components of breast milk (whole milk, skim milk, lipid layer, and breast-milk cells) had the highest sensitivity and concentration of human immunodeficiency virus (HIV) type 1 RNA burden and to determine biological correlates to these factors. The probability of detection of HIV (sensitivity) and the concentration of HIV-1 RNA were both associated with the choice of milk component, CD4(+) cell count, concentration of blood serum HIV-1 RNA, and the presence of breast inflammation. Whole milk demonstrated higher sensitivity and mean concentration than any other single component. Sensitivity was enhanced by analyzing all 4 components of breast milk.
Hoffman
Irving F
IF
Center for Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina 27599-7030, USA. hoffmani@med.unc.edu.
Martinson
Francis E A
FE
Stewart
Paul W
PW
Chilongozi
David A
DA
Leu
Szu-Yun
SY
Kazembe
Peter N
PN
Banda
Topia
T
Dzinyemba
Willard
W
Joshi
Priya
P
Cohen
Myron S
MS
Fiscus
Susan A
SA
D000328:Adult; D005260:Female; D015658:HIV Infections / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D007774:Lactation; D008413:Mastitis / Q000821:virology; D008895:Milk, Human / Q000737:chemistry* / Q000166:cytology / Q000821:virology*; D010865:Pilot Projects; D012367:RNA, Viral / Q000032:analysis*; D012680:Sensitivity and Specificity; D019562:Viral Load
D012367:RNA, Viral