[Relationship between the expression of IL-10 and liver regeneration following reduced-size orthotopic liver transplantation in rats].
OBJECTIVE
To explore the relationship between the expression of IL-10 and liver regeneration following reduced-size orthotopic liver transplantation in rats.


METHODS
Rats models with reduced-size orthotopic liver transplantation were established. The rats were divided in three groups: partial liver resection (I), orthotopic liver transplantations (II), and reduced-size orthotopic liver transplantation (III). The expression of IL-10 and regenerative response of liver in rats were evaluated by immunohistochemistry and flow cytometry on the 1st, 2nd, 4th and 7th days after the operations, respectively.


RESULTS
The liver grafts were capable of regeneration, the proliferation activity peaked on the fourth day with 26.3+/-0.9, 35.8+/-2.2, and 32.4+/-1.8 in I, II, and III groups, respectively. The expression of IL-10 was negative correlation to liver regeneration (r=-0.58, P<0.01).


CONCLUSIONS
Whole and reduced-size transplanted livers show the same regenerative activity. The maximal regenerative response delayes slightly, compared with that after partial hepatectomy. IL-10 plays an important immunomodulatory role in liver regeneration,and the effect is affected by general immune system and other cytokines.
Cheng
Feng
F
Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
Li
Xiang-cheng
XC
Wang
Xue-hao
XH
Qian
Xiao-feng
XF
Wang
Ke
K
Huang
Peng
P
D000818:Animals; D005434:Flow Cytometry; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D008115:Liver Regeneration; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D016753:Interleukin-10