title: Crystallization and preliminary X-ray analysis of class II fructose-1,6-bisphosphate aldolase from Thermus caldophilus.
abstract: In this study, we have crystallized class II fructose-1,6-bisphosphate aldolase (FBA) from Thermus caldophilus (Tca). Purified Tca FBA is a tetrameric enzyme of 305 residues, which crystallizes in the space group P2(1)2(1)2(1) (cell dimensions a = 98.9, b = 113.1, c = 115.7 A), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 A.
authors.affiliation: Department of Life Science, Kwangju Institute of Science & Technology, Gwangju 500-712, Korea.
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Im
authors.forename: Seong-Hwan
authors.initials: SH
authors.lastname: Rho
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Mun-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Tae-Yeon
authors.initials: TY
authors.lastname: Kim
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Oh
authors.forename: Hyun-Jae
authors.initials: HJ
authors.lastname: Shin
authors.forename: Dae-Sil
authors.initials: DS
authors.lastname: Lee
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000706:statistics & numerical data; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D013824:Thermus / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D005634:Fructose-Bisphosphate Aldolase