Proliferative activity of neokyotorphin-related hemoglobin fragments in cell cultures.
alpha-Hemoglobin fragments alpha-(133-141), alpha-(134-141), alpha-(135-141), alpha-(137-141), alpha-(134-140), alpha-(133-138), alpha-(134-140) and alpha-(137-138) stimulate L929 tumor cell proliferation, alpha-(134-141) being the most active. alpha-(134-141) stimulates proliferation of M3 melanoma cells, murine embryonic fibroblasts, primary cultures of red bone marrow and spleen cells. In L929 cells the effect of alpha-(134-141) is cell density independent; in M3 cells alpha-(137-141) and alpha-(134-141) are most active at density 10,000 cells/well (96 well plate) independently on FBS content.
Sazonova
Olga V
OV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117871 Moscow, Russia. sazonova@mail.ibch.ru
Blishchenko
Elena Yu
EY
Kalinina
Olga A
OA
Egorova
Natalya S
NS
Surovoy
Andrei Yu
AY
Philippova
Marina M
MM
Karelin
Andrei A
AA
Ivanov
Vadim T
VT
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D004723:Endorphins / Q000494:pharmacology*; D005914:Globins / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D051379:Mice; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016895:Culture Media, Serum-Free; D004723:Endorphins; D006454:Hemoglobins; D009842:Oligopeptides; D010446:Peptide Fragments; C036450:neo-kyotorphin; D005914:Globins