DNA sequence and analysis of the pYVa127/90 virulence plasmid of Yersinia enterocolitica strain A127/90.
The complete nucleotide sequence and organization of the Yersinia enterocolitica strain A127/90 serotype O:8 biotype 1B virulence plasmid (pYVa127/90) were determined. In contrast to other biotype 1B strains, usually isolated in the USA, strain A127/90 was isolated in Japan. The organization of the genes carried by the 66591-bp pYVa127/90 plasmid is globally similar to that of 67720-bp pYVe8081, the virulence plasmid from 8081, an American isolate of serotype O:8 biotype 1B. Differences are noticeable in the amino acid sequences of the SycH chaperone, the YopM cytotoxin and the plague-protective antigen LcrV. Interestingly, pYVa127/90 contains the entire coding sequence of the YlpA lipoprotein, which is missing from pYVe8081. In addition, we identified five potential new genes. Several vestigial insertion sequence elements appeared to be different between the two plasmids. These observations suggest that both pYV plasmids are from a common ancestor but may have evolved independently following the conquest of two distant ecological niches.
Foultier
Boris
B
Microbial Pathogenesis Unit, Christian de Duve Institute of Cellular Pathology and Faculté de Médecine, Université catholique de Louvain, 1200 Brussels, Belgium.
Cornelis
Guy R
GR
D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D004261:DNA Replication; D023061:Gene Order; D020071:Interspersed Repetitive Sequences; D008074:Lipoproteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D052899:Pore Forming Cytotoxic Proteins; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C043908:LcrV protein, Yersinia; D008074:Lipoproteins; D018832:Molecular Chaperones; D052899:Pore Forming Cytotoxic Proteins; C090049:SycH protein, Yersinia; D037521:Virulence Factors; C060772:yopM protein, Yersinia; C067378:ylpA protein, Yersinia enterocolitica