Functional activities and cellular localization of the ezrin, radixin, moesin (ERM) and RING zinc finger domains in MIR.
Myosin regulatory light chain interacting protein (MIR) belongs to the ezrin, radixin, moesin (ERM) family of proteins involved in membrane cytoskeleton interactions and cell dynamics. MIR contains, beside the ERM domain, a RING zinc finger region. Immunocytochemistry showed that full-length MIR and the subdomains localize differently in cells. Cell fractionation revealed a similar distribution of full-length MIR and the RING domain protein in the Triton X-100-insoluble fraction. The neurite outgrowth inhibitory activity of MIR was attributed to the RING domain. MIR levels were controlled in the cells depending on the intact RING domain and proteasome activity. The dynamic regulation of MIR contributes to its effects on neurite outgrowth and cell motility.
Bornhauser
Beat C
BC
Department of Neuroscience, Neurobiology, Uppsala University, Biomedical Centre, Sweden.
Johansson
Cecilia
C
Lindholm
Dan
D
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D016501:Neurites / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers; D000099149:Ezrin
D001798:Blood Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases; D000099149:Ezrin; C071058:moesin; C071776:radixin; C403126:MYLIP protein, human