title: Lupin nad9 and nad6 genes and their expression: 5' termini of the nad9 gene transcripts differentiate lupin species.
abstract: The mitochondrial nad9 and nad6 genes were analyzed in four lupin species: Lupinus luteus, Lupinus angustifolius, Lupinus albus and Lupinus mutabilis. The nucleotide sequence of these genes confirmed their high conservation, however, higher number of nucleotide substitution was observed in the L. albus genes. Southern hybridizations confirmed the presence of single copy number of these genes in L. luteus, L. albus and L. angustifolius. The expression of nad9 and nad6 genes was analyzed by Northern in different tissue types of analyzed lupin species. Transcription analyses of the two nad genes displayed single predominant mRNA species of about 0.6 kb in L. luteus and L. angustifolius. The L. albus transcripts were larger in size. The nad9 and nad6 transcripts were modified by RNA editing at 8 and 11 positions, in L. luteus and L. angustifolius, respectively. The gene order, rps3-rpl16-nad9, found in Arabidopsis thaliana is also conserved in L. luteus and L. angustifolius mitochondria. L. luteus and L. angustifolius showed some variability in the sequence of the nad9 promoter region. The last feature along with the differences observed in nad9 mRNA 5' termini of two lupins differentiate L. luteus and L. angustifolius species.
authors.affiliation: Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, A. Mickiewicz University, 5 Miedzychodzka Str., 60-371 Poznań, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Rurek
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Nuc
authors.forename: Katarzyna Dorota
authors.initials: KD
authors.lastname: Raczyńska
authors.forename: Halina
authors.initials: H
authors.lastname: Augustyniak
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D031297:Lupinus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077278:RNA, Mitochondrial; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D020121:5' Untranslated Regions; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D021122:Protein Subunits; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012313:RNA; D009245:NADH Dehydrogenase