title: Interactions of potent multisubstrate analogue inhibitors with purine nucleoside phosphorylase from calf spleen--kinetic and spectrofluorimetric studies.
abstract: Dissociation constants and stoichiometry of binding for interaction of trimeric calf spleen purine nucleoside phosphorylase with potent multisubstrate analogue inhibitors were studied by kinetic and spectrofluorimetric methods.
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Warsaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Iwanow
authors.forename: Lucyna
authors.initials: L
authors.lastname: Magnowska
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yokomatsu
authors.forename: Shiroshi
authors.initials: S
authors.lastname: Shibuya
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bzowska
mesh_terms: D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000493:pharmacokinetics* / Q000494:pharmacology; D007700:Kinetics; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D011683:Purine-Nucleoside Phosphorylase