title: The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160.
abstract: In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direct coupling to an RNA binding protein Scp160. These findings suggest an additional role for Gpa1 and reveal Scp160 as a component of the mating response pathway in yeast.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.forename: Christopher
authors.initials: C
authors.lastname: Aston
authors.forename: Scott A
authors.initials: SA
authors.lastname: Burchett
authors.forename: Christine
authors.initials: C
authors.lastname: Dyke
authors.forename: Stanley
authors.initials: S
authors.lastname: Fields
authors.forename: S Johannes R
authors.initials: SJ
authors.lastname: Rajarao
authors.forename: Peter
authors.initials: P
authors.lastname: Uetz
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Wang
authors.forename: Kathleen
authors.initials: K
authors.lastname: Young
authors.forename: Henrik G
authors.initials: HG
authors.lastname: Dohlman
mesh_terms: D004305:Dose-Response Relationship, Drug; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism* / Q000502:physiology*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D005690:Galactose / Q000378:metabolism; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D008565:Membrane Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C097262:SCP160 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D005982:Glutathione Transferase; C084293:GPA1 protein, S cerevisiae; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D005947:Glucose; D005690:Galactose