Subsite structure of maltogenic amylase from Thermomonospora viridis TF-35.
We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A(-3), A(-2), (A(-1)+A(+1)), A(+2) and A(+3) were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol(-1), respectively.
Takahashi
Kosei
K
Nihon Shokuhin Kako Co., Ltd., Fuji-shi, Shizuoka, Japan. kosei.takahashi@nisshoku.co.jp
Nakakuki
Teruo
T
D000192:Actinomycetales / Q000201:enzymology*; D001665:Binding Sites; D020134:Catalytic Domain; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics
D009844:Oligosaccharides; C021705:maltooligosaccharides; D006026:Glycoside Hydrolases; C120909:glucan 1,4-alpha-maltohydrolase