title: Pheophorbide A-methyl ester, Acyl-CoA: cholesterol acyltransferase inhibitor from Diospyros kaki.
abstract: In the course of our search for Acyl-CoA: cholesterol acyltransferase (ACAT) inhibitors from natural sources, a new type of ACAT inhibitor was isolated from a methanol extract of Diospyros kaki. On the basis of spectral and structural evidence, the compound was identified as pheophorbide A-methyl ester. Pheophorbide A-methyl ester inhibited ACAT activity in a dose dependent manner with an IC50 value of 1.85 microg/mL.
authors.affiliation: Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Mi Yeon
authors.initials: MY
authors.lastname: Chung
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Song
authors.forename: Oh Eok
authors.initials: OE
authors.lastname: Kwon
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Baek
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Jeune
authors.forename: Koanhoi
authors.initials: K
authors.lastname: Kim
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Young Kook
authors.initials: YK
authors.lastname: Kim
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics; D000818:Animals; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029788:Diospyros / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D008401:Gas Chromatography-Mass Spectrometry; D007723:Korea; D000432:Methanol; D008738:Methyl Ethers; D008862:Microsomes, Liver / Q000201:enzymology; D015394:Molecular Structure; D010936:Plant Extracts / Q000493:pharmacokinetics* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification* / Q000493:pharmacokinetics
chemical_list: D000214:Acyl Coenzyme A; D004365:Drugs, Chinese Herbal; D008738:Methyl Ethers; D010936:Plant Extracts; D002734:Chlorophyll; D002785:Sterol O-Acyltransferase; C032623:pheophorbide a; D000432:Methanol