[Biocompatibility of poly-l-lysine-modified silica nanoparticles].
OBJECTIVE
Poly-l-lysine-modified silica nanoparticle(PMS-NP) was a novel non-viral vector for gene delivery. The current study was designed to evaluate the biocompatibility of PMS-NP for its further utilization in vivo.


METHODS
Cell transfection and flow cytometry were used to elucidate the delivery efficiency of plasmid DNA and antisense ODN mediated by PMS-NP in the presence of serum-containing medium. Subsequently, the biocompatibility of PMS-NP in vivo was evaluated using filtration assay of plasma proteins and erythrocyte aggregation assay.


RESULTS
The abilities of PMS-NP to deliver plasmid DNA and antisense ODN in vitro clearly decreased in the presence of serum-containing medium. PMS-NP/DNA(ODN)complexes bound plasma proteins and triggered erythrocyte aggregation.


CONCLUSIONS
PMS-NP might interact with plasma proteins, resulting in decreased transfection efficiency in vitro. And filtration assay of plasma proteins and the erythrocyte aggregation assay demonstrated that the interaction of PMS-NP with plasma proteins and erythrocytes might play a negative role in gene transfection efficiency in vivo. And its biocompatibility needs to be further improved.
Zhu
Shi-Guo
SG
Cancer Research Institute, Central South University, Changsha, Hunan, 410078, PR China.
Gan
Kai
K
Li
Zheng
Z
Shen
Shou-Rong
SR
Xiang
Juan-Juan
JJ
Li
Xiao-Ling
XL
Fan
Shong-Qing
SQ
Lu
Hong-Bin
HB
Zeng
Zhao-Yang
ZY
Li
Gui-Yuan
GY
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage*; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage; D010957:Plasmids; D011107:Polylysine; D011485:Protein Binding; D012822:Silicon Dioxide / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods*
D001798:Blood Proteins; D016895:Culture Media, Serum-Free; D020319:Oligodeoxyribonucleotides, Antisense; D011107:Polylysine; D012822:Silicon Dioxide