Boric acid reversibly inhibits the second step of pre-mRNA splicing.
Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3' splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.
Shomron
Noam
N
Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Ramat Aviv, Israel.
Ast
Gil
G
D001888:Boric Acids / Q000494:pharmacology*; D001897:Boronic Acids / Q000737:chemistry / Q000494:pharmacology; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008670:Metals / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism*; D012326:RNA Splicing / Q000187:drug effects*; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000187:drug effects / Q000378:metabolism
D001888:Boric Acids; D001897:Boronic Acids; D008670:Metals; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins; D017411:Ribonucleoproteins, Small Nuclear; C490656:SLU7 protein, human; D005914:Globins; C032688:boric acid