Roles of conserved nucleotide-binding domains in accessory proteins, HypB and UreG, in the maturation of nickel-enzymes required for efficient Helicobacter pylori colonization.
Helicobacter pylori synthesizes two nickel-containing enzymes (urease and hydrogenase), both of which are important pathogenesis factors. Among the many accessory proteins needed for maturation of these Ni-enzymes, are two proteins, HypB and UreG, each of which contain a conserved nucleotide-binding domain (GSGKT). To address the role of this domain in the maturation process, site-directed mutations were introduced in both hypB and ureG. The hypB site-directed mutant strain (Lys59 to Ala59) lacked hydrogenase activity and had less than 1% of the parental urease activity. Hydrogenase activity was partially, and urease activity was fully restored in the hypB mutant strain when grown on nickel supplemented media. The hydrogenase activity of the ureG site-directed mutant strain (Lys14 to Ala14) was comparable to that of the parental strain. However, the ureG mutant strain lacked urease activity, and this deficiency could not be suppressed even when the strain was grown on nickel supplemented media. The expression of immunologically detectable HypB and UreG in the mutants was similar to the parental strain. Expression of the UreA and UreB subunits of urease in both the mutants was also normal. Purified UreG parental and mutant (Lys14 to Ala14) proteins had molecular masses of 27 kDa, but possessed negligible GTP hydrolyzing activity.
Mehta
Nalini
N
Department of Microbiology, University of Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA.
Benoit
Stéphane
S
Maier
Robert J
RJ
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006160:Guanosine Triphosphate / Q000378:metabolism; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009532:Nickel / Q000378:metabolism; D028044:Phosphate-Binding Proteins; D017354:Point Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D014510:Urease / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D002352:Carrier Proteins; C089637:HypB protein, bacteria; D028044:Phosphate-Binding Proteins; D021122:Protein Subunits; D037521:Virulence Factors; C082270:ureG protein, Bacteria; D009532:Nickel; D006160:Guanosine Triphosphate; D006864:Hydrogenase; D014510:Urease; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins