title: Influence of anticoagulants on the measurement of S100B protein in blood.
abstract: OBJECTIVE
  Evaluate anticoagulants influence on plasma S100B levels.
  
  
  METHODS
  Blood were collected from 18 healthy adult subjects using: no anticoagulants, EDTA, heparin, and citrate. S100B levels were determined using LIA-mat assay.
  
  
  RESULTS
  Heparin plasma and citrate increased plasma S100B levels (p < 0.001), whereas EDTA had no effect (p = 0.24). Heparin plasma samples were highly (r2 = 0.97, p < 0.001), citrate samples were moderately (r2 = 0.49, p < 0.001), and EDTA samples were not (r2 = 0.22, p = 0.059) correlated with serum samples.
  
  
  CONCLUSIONS
  When anticoagulant is required, heparin plasma should be the primary choice for measurement of S100 B levels.
authors.affiliation: Departamento de Bioquímica, ICBS, UFRGS, Brazil.
authors.forename: Adriano B L
authors.initials: AB
authors.lastname: Tort
authors.forename: Marcelo O
authors.initials: MO
authors.lastname: Dietrich
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Diogo O
authors.initials: DO
authors.lastname: Souza
authors.forename: Luis V C
authors.initials: LV
authors.lastname: Portela
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D016477:Artifacts; D019343:Citric Acid; D004492:Edetic Acid; D005260:Female; D006493:Heparin; D006801:Humans; D008297:Male; D009418:S100 Proteins / Q000097:blood*
chemical_list: D000925:Anticoagulants; D009418:S100 Proteins; D019343:Citric Acid; D006493:Heparin; D004492:Edetic Acid