Study of the factors affecting the forward and back extraction of yeast-lipase and its activity by reverse micelles.
The experimental results of yeast-lipase extracted by AOT-reverse-micelles in isooctane are reported. The effects of pH values and ionic strength in the aqueous phase, surfactant concentration, phase volume ratio, temperature, stirring time, and cosolvent concentration on the phase transfer of the lipase by the reverse micelles are studied. One hundred percent of forward extraction yield, 68% of back extraction yield, and 45% of activity recovery yield for the lipase are obtained. The phase transfer of the lipase is controlled by electrostatic and hydrophobic interactions.
Yu
Yan-chun
YC
Department of Chemical Engineering, Huaihai Institute of Technology, Lanyungang 222005, People's Republic of China. yyc@hhit.edu.cn
Chu
Ying
Y
Ji
Jian-Yie
JY
D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification*; D008823:Micelles; D009795:Octanes / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry; D015003:Yeasts / Q000201:enzymology*
D008823:Micelles; D009795:Octanes; D013501:Surface-Active Agents; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; D000431:Ethanol; D008049:Lipase; C045798:2,2,4-trimethylpentane