[Transformation of bar gene to tetraploid of Isatis indigotica].
The transgenic tetraploid of Isatis indigotica mediated by Agrobacterium tumefaciens was obtained. To transfer the plant binary expression vector pCAMBIA 3300 carrying bar gene, the Agrobacterium tumefaciens strain EHA 105 was used as engineering bacterium. The results of PCR indicated that the bar gene had been transferred into and merged with the genome of Isatis indigotica. This study will make foundation for improvement of other characters of this species with genetic engineering.
Xu
Tiefeng
T
Pharmacy College, Second Military Medical University, Shanghai 200433.
Tang
Kexuan
K
Zhang
Hanming
H
Guo
Meili
M
Zhang
Lei
L
Li
Fupeng
F
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D005798:Genes, Bacterial; D005818:Genetic Engineering; D031219:Isatis / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000235:genetics / Q000254:growth & development; D011123:Polyploidy; D014170:Transformation, Genetic