2'-O,4'-C-ethylene-bridged nucleic acid (ENA) for effective antisense formation.
ENA antisense oligonucleotides for vascular endothelial growth factor (VEGF) mRNA were synthesized and evaluated in A549 lung cancer cells. It was found that the VEGF ENA-antisense inhibited not only the expression of VEGF, but also the expression of three genes, which were found in Genbank by BLAST and Clustal W search and considered likely to bind to the VEGF ENA-antisense. These results indicate that ENA-antisense oligonucleotides act in a sequence-specific manner, and could be used as effective antisense drugs.
Morita
Koji
K
Exploratory Chemistry Research Laboratories, Sankyo Co., Limited, Tokyo, Japan.
Yamate
Koji
K
Kurakata
Shin-ichi
S
Watanabe
Kenji
K
Imanishi
Takeshi
T
Koizumi
Makoto
M
D002460:Cell Line; D005030:Ethylenes; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007202:Indicators and Reagents; D008175:Lung Neoplasms; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis* / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D005030:Ethylenes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C036216:ethylene