Ribotyping characterisation of campylobacter isolates randomly collected from different sources in Italy.
In this study the potential for using the automated PstI ribotyping as a primary library typing method to survey Campylobacter and for identification of two thermophilic Campylobacter jejuni and Campylobacter coli species was evaluated. A total of 158 isolates randomly collected in Italy from different sources were analyzed. A large percentage of chicken (28%), turkey (27%) and turkey meat (25%) isolates shared their ribotyping profiles (ribotypes) with those of humans, whereas the swine isolates had unique profiles. The identification results obtained by ribotyping corresponded to those collected by using a multiplex PCR protocol specifically designed for C. jejuni and C. coli detection. The comparison of the PstI ribotyping profiles obtained in this research with those of the isolates collected over time will facilitate determining the ribotypes that are more frequently transmitted to humans in comparison to those that are normally harboured only in animals, foods and in the environment.
Manfreda
Gerardo
G
Department of Food Science, Alma Mater Studiorum, University of Bologna, Via S. Giacomo 9, 40126 Bologna, Italy. manfreda@alma.unibo.it
De Cesare
Alessandra
A
Bondioli
Valentina
V
Franchini
Achille
A
D000003:Abattoirs; D000818:Animals; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D016000:Cluster Analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D013552:Swine / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology
C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific