title: Inhibitory effect of kaurane type diterpenoids from Acanthopanax koreanum on TNF-alpha secretion from trypsin-stimulated HMC-1 cells.
abstract: Five known kaurane type diterpenoids, 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1), 16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16alpha-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity (IC50 value, 16.2 uM) on TNF-alpha secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Xing Fu
authors.initials: XF
authors.lastname: Cai
authors.forename: Guanghai
authors.initials: G
authors.lastname: Shen
authors.forename: Nguyen Tien
authors.initials: NT
authors.lastname: Dat
authors.forename: Ok Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jung Joon
authors.initials: JJ
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D028001:Eleutherococcus / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D007723:Korea; D007946:Leukemia, Mast-Cell / Q000139:chemically induced / Q000378:metabolism; D047311:Luteolin; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D014357:Trypsin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: C487399:16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid; D045786:Diterpenes, Kaurane; D005419:Flavonoids; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D014357:Trypsin; D047311:Luteolin; D000432:Methanol