title: Tri16 is required for esterification of position C-8 during trichothecene mycotoxin production by Fusarium sporotrichioides.
abstract: We previously characterized Tri1, a gene required for hydroxylation of the C-8 position during trichothecene mycotoxin biosynthesis in Fusarium sporotrichioides NRRL 3299. Sequence analysis of the region surrounding Tri1 revealed a gene, named Tri16, which could encode an acyltransferase. Unlike the wild-type parent strain NRRL 3299, which accumulates primarily T-2 toxin along with low levels of diacetoxyscirpenol (DAS) and neosolaniol (NEO) and trace amounts of 8-propionyl-neosolaniol (P-NEO) and 8-isobutyryl-neosolaniol (B-NEO), mutants containing a disruption of Tri16 were blocked in the production of the three C-8 esterified compounds T-2 toxin, P-NEO, and B-NEO and accumulated the C-8-hydroxylated compound NEO along with secondary levels of DAS. These data indicate that Tri16 encodes an acyltransferase that catalyzes the formation of ester side groups at C-8 during trichothecene biosynthesis. We also report the presence of a Tri16 ortholog in Gibberella pulicaris R-6380 that is likely linked to a presumably inactive ortholog for Tri1.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Peplow
authors.forename: Isaac B
authors.initials: IB
authors.lastname: Meek
authors.forename: Melinda C
authors.initials: MC
authors.lastname: Wiles
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Phillips
authors.forename: Marian N
authors.initials: MN
authors.lastname: Beremand
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D004951:Esterification; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005874:Gibberella / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013605:T-2 Toxin / Q000096:biosynthesis*; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014255:Trichothecenes; C016009:neosolaniol; D000217:Acyltransferases; D013605:T-2 Toxin