title: Detection of circulating MN/CA9 positive renal cell carcinoma cells during operation.
abstract: Our previous study demonstrated the clear detection of MN/CA9 mRNA in peripheral blood samples from RCC patients. However, approximately 30 % of control blood samples from healthy volunteers showed MN/CA9 expression. We have developed a new primer set and optimized the PCR conditions, now resulting in a specificity of 100 %. In tissue samples, all clear cell type carcinomas but none of the spindle cell type and pleomorphic cell type tumors expressed the MN/CA9 message. Analysis of MN/CA9 messages in peripheral blood samples from RCC patients gave positive results for 0/2, 1/9, 0/4 and 4/12 of stage I, II, III and IV cases, respectively. RT-PCR analysis using preoperative renal venous blood samples resulted in clear detection of MN/CA9 positive cells in 2/4, 3/13, 2/6 and 1/1 of stage I, II, III and IV cases, respectively. Our results suggest that assessment of MN/CA9 expression by RT-PCR is a promising method for detecting cancer cells in the circulation of patients with RCC.
authors.affiliation: Department of Urology, Nara Medical University, Kashihara, Japan. huemura@naramed-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Uemura
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: A
authors.initials: A
authors.lastname: Iwai
authors.forename: E
authors.initials: E
authors.lastname: Okajima
authors.forename: E
authors.initials: E
authors.lastname: Okajima
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Hirao
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000097:blood / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000235:genetics / Q000601:surgery; D018076:DNA, Complementary; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000235:genetics / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics*; D009360:Neoplastic Cells, Circulating; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases