Suppressive effects of genistein on oxidative stress and NFkappaB activation in RAW 264.7 macrophages.
This study was designed to investigate whether genistein may ameliorate oxidative stress and nuclear factor kappaB (NFkappaB) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with genistein significantly reduced lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in a dose-dependent manner with an IC50 of 69.4 microM. Genistein at 50 microM and 100 microM concentrations reduced thiobarbituric acid-reactive substances (TBARS) accumulation, increasing the GSH level and antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase. The specific DNA-binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from 50 microM and 100 microM genistein treatments were significantly suppressed. These results suggest that genistein has mild antioxidant activity to suppress intracellular oxidative stress and NFkappaB activation.
Choi
Chunyeon
C
School of Food and Life Science, Food Science Institute and Biohealth Product Research Center, Inje University, Kimhae, Korea.
Cho
Hyeyeon
H
Park
Jiyoung
J
Cho
Chungwon
C
Song
Youngsun
Y
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008070:Lipopolysaccharides; D016328:NF-kappa B; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D019833:Genistein; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione