The porcine skin associated T-cell homing chemokine CCL27: molecular cloning and mRNA expression in piglets infected experimentally with Staphylococcus hyicus.
CCL27 (also named CTACK, ALP, ILC and ESkine) is a CC chemokine primarily expressed by keratinocytes of the skin. The cognate receptor of CCL27 named CCR10 (GPR-2), is also expressed in skin-derived cells, and in addition by a subset of peripheral blood T-cells and in a variety of other tissues. In this paper, we report the cloning of porcine CCL27 cDNA and investigation of CCL27 mRNA expression in Staphylococcus hyicus infected piglets. At the protein level, 77 and 74% homology was found to human and mouse CCL27 sequences, respectively. The results of the expression analyses show that CCL27 mRNA is upregulated in the skin of infected piglets and to a lesser extent in piglets recovered from disease and without clinical signs of infection, indicating a role for CCL27 both during inflammation and after recovery from an infection.
Johnsen
Christina K
CK
Department of Immunology and Biochemistry, Danish Veterinary Institute, Bülowsvej 27, DK-1790 Copenhagen, Denmark. cjo@ssi.dk
Jensen
Annette N
AN
Ahrens
Peter
P
Andresen
Lars Ole
LO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001706:Biopsy / Q000662:veterinary; D054425:Chemokine CCL27; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology; D004818:Epidermitis, Exudative, of Swine / Q000276:immunology* / Q000382:microbiology; D005083:Exfoliatins / Q000276:immunology; D005786:Gene Expression Regulation; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013207:Staphylococcal Skin Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000276:immunology*; D013552:Swine
C492276:CCL27 protein, human; D054425:Chemokine CCL27; D019742:Chemokines, CC; D005083:Exfoliatins; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D003208:Concanavalin A; D018664:Interleukin-12