Post-TATA binding protein recruitment clearance of Gcn5-dependent histone acetylation within promoter nucleosomes.
Transcriptional activation of eukaryotic genes often requires the function of histone acetyltransferases (HATs), which is expected to result in the hyperacetylation of histones within promoter nucleosomes. In this study we show that, in Saccharomyces cerevisiae, the steady-state levels of Gcn5-dependent histone acetylation within a number of transcriptionally active promoters are inversely related to the rate of transcription. High acetylation levels were measured only when transcription was attenuated either by TATA element mutations or in a strain carrying a temperature-sensitive protein component of RNA polymerase II. In addition, we show that in one case the low levels of histone acetylation depend on the function of the Rpd3 histone deacetylase. These results point to the existence of an unexpected interplay of two opposing histone-modifying activities which operate on promoter nucleosomes following the initiation of RNA synthesis. Such interplay could ensure rapid turnover of chromatin acetylation states in continuously reprogrammed transcriptional systems.
Topalidou
Irini
I
Institute of Molecular Biology and Biotechnology, FORTH, Heraklion 711 10, Crete, Greece.
Papamichos-Chronakis
Manolis
M
Thireos
George
G
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000135:Acid Phosphatase / Q000235:genetics; D000878:Anthranilate Synthase / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D016385:TATA Box / Q000235:genetics; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D000135:Acid Phosphatase; D000878:Anthranilate Synthase; D004268:DNA-Binding Proteins; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases; D006657:Histones; D007209:Indole-3-Glycerol-Phosphate Synthase; D009097:Multienzyme Complexes; D009707:Nucleosomes; D011494:Protein Kinases; D012319:RNA Polymerase II; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C071665:RPD3 protein, S cerevisiae; C087439:TRP3 protein, S cerevisiae