Facile and selective nanoscale labeling of peptides in solution by using photolabile protecting groups.
For the selective labeling of peptides, a novel strategy was developed that combines the advantages of solid-phase peptide synthesis with the flexibility of labeling reactions in solution. To direct a label at a distinct position within the peptide sequence, other reactive positions are blocked with photolabile protecting groups that could be easily removed after the labeling reaction. Therefore selective labeling may become feasible for the first time even in nanomol amounts.
Koglin
Norman
N
Institute of Biochemistry, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.
Lang
Manja
M
Rennert
Robert
R
Beck-Sickinger
Annette G
AG
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000138:chemical synthesis; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D036103:Nanotechnology; D009478:Neuropeptide Y / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D017476:Receptors, Neuropeptide Y / Q000187:drug effects / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D005449:Fluorenes; D007202:Indicators and Reagents; D009478:Neuropeptide Y; D010455:Peptides; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor; C054007:1-(9-fluorenyl)methyl chloroformate; D015740:Calcitonin Gene-Related Peptide