Synthesis of an octamannosyled glycan chain, the key oligosaccharide structure in ER-associated degradation.
The high-mannose type decasaccharide (Man(8)GlcNAc(2)), the proposed ligand of ER residing mannosidase-like proteins (MLP), and its monoglycosylated homologue (alpha-Glc(1)Man(8)GlcNAc(2)) were synthesized. The oligosaccharide assembly was performed in a convergent and stereoselective manner, using three oligosaccharide components, a core trisaccharide having a beta-mannoside bond, a liner mannotriose, and a branched mannotetraose.
Matsuo
Ichiro
I
RIKEN, The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Ito
Yukishige
Y
D002240:Carbohydrate Sequence; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D008024:Ligands; D008358:Mannose / Q000737:chemistry*; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis*
D008024:Ligands; D009844:Oligosaccharides; D011134:Polysaccharides; D008361:Mannosidases; D008358:Mannose