title: Endcaps for stabilizing short DNA duplexes.
abstract: The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA.
authors.forename: Pei-Sze
authors.initials: PS
authors.lastname: Ng
authors.forename: Maneesh R
authors.initials: MR
authors.lastname: Pingle
authors.forename: Ganesan
authors.initials: G
authors.lastname: Balasundarum
authors.forename: Alan
authors.initials: A
authors.lastname: Friedman
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zu
authors.forename: Donald E
authors.initials: DE
authors.lastname: Bergstrom
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013816:Thermodynamics
chemical_list: D004247:DNA