title: Mechanisms of mitochondria-neurofilament interactions.
abstract: Mitochondria are localized to regions of the cell where ATP consumption is high and are dispersed according to changes in local energy needs. In addition to motion directed by molecular motors, mitochondrial distribution in neuronal cells appears to depend on the docking of mitochondria to microtubules and neurofilaments. We examined interactions between mitochondria and neurofilaments using fluorescence microscopy, dynamic light scattering, atomic force microscopy, and sedimentation assays. Mitochondria-neurofilament interactions depend on mitochondrial membrane potential, as revealed by staining with a membrane potential sensitive dye (JC-1) in the presence of substrates/ADP or uncouplers (valinomycin/carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone) and are affected by the phosphorylation status of neurofilaments and neurofilament sidearms. Antibodies against the neurofilament heavy subunit disrupt binding between mitochondria and neurofilaments, and isolated neurofilament sidearms alone interact with mitochondria, suggesting that they mediate the interactions between the two structures. These data suggest that specific and regulated mitochondrial-neurofilament interactions occur in situ and may contribute to the dynamic distribution of these organelles within the cytoplasm of neurons.
authors.affiliation: Institute for Medicine and Engineering, Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6383, USA. owagner@mail.med.upenn.edu
authors.forename: O I
authors.initials: OI
authors.lastname: Wagner
authors.forename: J
authors.initials: J
authors.lastname: Lifshitz
authors.forename: P A
authors.initials: PA
authors.lastname: Janmey
authors.forename: M
authors.initials: M
authors.lastname: Linden
authors.forename: T K
authors.initials: TK
authors.lastname: McIntosh
authors.forename: J-F
authors.initials: JF
authors.lastname: Leterrier
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001667:Binding, Competitive / Q000502:physiology; D002417:Cattle; D003599:Cytoskeleton / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020407:Internet; D008564:Membrane Potentials / Q000502:physiology; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D016900:Neurofilament Proteins / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D013116:Spinal Cord / Q000737:chemistry; D014741:Video Recording
chemical_list: D000906:Antibodies; D016900:Neurofilament Proteins; C044882:neurofilament protein L; C051774:neurofilament protein H; C061195:neurofilament protein M