title: MRI of blood volume with MS 325 in experimental choroidal melanoma.
abstract: Functional magnetic resonance imaging (MRI) allows quantitative blood volume imaging in vivo at high tissue resolution. The purpose is to apply this technique for untreated and hyperthermia-treated experimental choroidal melanoma. MS 325 was used as new intravascular albumin-bound gadolinium-based contrast agent. Pigmented choroidal melanomas were established in albino rabbits. MRI was performed in 7 untreated eyes and 7 eyes treated with a Neodymium:Yttrium-Lanthanum-Fluoride-laser at 1047 nm. 3D-spoiled gradient echo pulse sequences were used to acquire T' weighted axial images. First, a set of images was collected without contrast agent. MS 325 was then injected i.v. and images were obtained within 12 min after injection. Signal intensities were measured within tumor, ciliary body, choroid, and iris and relative signal intensities were determined for these tissues in relation to vitreous. In untreated tumors, the relative signal intensity was higher after injection of MS 325 (5.61+0.70) than without MS 325 (2.90+0.33; p = 0.0002). In contrast, the relative signal intensity of treated tumors did not differ significantly before and after MS 325 (6.19+1.59 and 6.13+1.64). Histopathological sections indicated vascular occlusion in treated tumors. All other studied tissues of untreated and treated eyes showed a significant increase of relative signal intensities in the presence of MS 325. An animal model for the research on contrast agents in MRI is presented. Blood volume measurement with MS 325 was adapted for experimental choroidal melanomas. Reduced change of relative signal intensity indicates compromised blood volume after vascular occlusion in hyperthermia-treated melanoma. Further studies are needed to investigate whether this technique allows the evaluation of tumor viability following treatments.
authors.affiliation: Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Matthias H J
authors.initials: MH
authors.lastname: Krause
authors.forename: Kenneth K
authors.initials: KK
authors.lastname: Kwong
authors.forename: Jing
authors.initials: J
authors.lastname: Xiong
authors.forename: Evangelos S
authors.initials: ES
authors.lastname: Gragoudas
authors.forename: Lucy H Y
authors.initials: LH
authors.lastname: Young
mesh_terms: D000818:Animals; D001810:Blood Volume; D002830:Choroid Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy; D003287:Contrast Media; D005260:Female; D005682:Gadolinium; D006979:Hyperthermia, Induced; D053685:Laser Therapy; D008546:Melanoma, Experimental / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy; D009942:Organometallic Compounds; D011817:Rabbits
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; D005682:Gadolinium; C109932:gadofosveset trisodium