title: RNase H activation by stereoregular boranophosphate oligonucleotide.
abstract: A stereoregular all-(Sp)-boranophosphate oligodeoxyribonucleotide (BH3(-)-ODN) 15-mer was synthesized using an enzymatic approach. The BH3(-)-ODN formed a hybrid with the complementary RNA 15-mer and induced RNase H hydrolysis of the RNA strand at ODN concentrations as low as 10 nM at 37 degrees C, but with a lower efficiency than that of its natural phosphodiester analogue.
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina 27708-0346, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Mikhail
authors.initials: M
authors.lastname: Dobrikov
authors.forename: Dmitri
authors.initials: D
authors.lastname: Sergueev
authors.forename: Barbara Ramsay
authors.initials: BR
authors.lastname: Shaw
mesh_terms: D001483:Base Sequence; D001896:Boron Compounds; D004789:Enzyme Activation; D018734:Genes, erbB-2; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D016914:Ribonuclease H / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001896:Boron Compounds; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D016914:Ribonuclease H