title: A simple rapid method of sample preparation for LC analysis of retinoyl beta-glucuronide and retinoic acid in water-based creams.
abstract: A simple method of sample preparation for LC analysis of retinoic acid (RA) and retinoyl beta-glucuronide (RAG) in creams has been developed. Water-based cream of all trans-RAG, devoid of side effects but efficacious in the treatment of acne vulgaris, was found to be hydrolyzed to RA in a temperature dependant manner. The potential benefits of water-based RAG cream stored at room temperature for the treatment of acne and wrinkle is discussed.
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 356 Molecular Biology Building, Ames, IA 50011, USA. abarua@iastate.edu
authors.forename: Arun B
authors.initials: AB
authors.lastname: Barua
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid; D006868:Hydrolysis; D007641:Keratolytic Agents / Q000032:analysis*; D009824:Ointments; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D007641:Keratolytic Agents; D009824:Ointments; C026787:retinoyl glucuronide; D014212:Tretinoin