title: B7-H1 blockade augments adoptive T-cell immunotherapy for squamous cell carcinoma.
abstract: In this report, we demonstrate that B7-H1, a B7 family molecule implicated in tumor immune evasion, is constitutively expressed on 66% of freshly isolated squamous cell carcinomas of the head and neck (SCCHN). To define the potential impact of tumor-associated B7-H1 on immunotherapy, the B7-H1-negative mouse SCC line, SCCVII, was transfected to express B7-H1. Although all of the animals succumbed to B7-H1/SCCVII tumors even after adoptive T-cell immunotherapy, the infusion of B7-H1 blocking monoclonal antibody with activated T cells cured 60% of animals. These data support B7-H1 blockade as a new approach to enhance the efficacy of T-cell immunotherapy.
authors.affiliation: Department of Otolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. strome.scott@mayo.edu
authors.forename: Scott E
authors.initials: SE
authors.lastname: Strome
authors.forename: Haidong
authors.initials: H
authors.lastname: Dong
authors.forename: Hideto
authors.initials: H
authors.lastname: Tamura
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Voss
authors.forename: Dallas B
authors.initials: DB
authors.lastname: Flies
authors.forename: Koji
authors.initials: K
authors.lastname: Tamada
authors.forename: Diva
authors.initials: D
authors.lastname: Salomao
authors.forename: John
authors.initials: J
authors.lastname: Cheville
authors.forename: Fumiya
authors.initials: F
authors.lastname: Hirano
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Jan L
authors.initials: JL
authors.lastname: Kasperbauer
authors.forename: Karla V
authors.initials: KV
authors.lastname: Ballman
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D060890:B7-H1 Antigen; D001798:Blood Proteins; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; D001798:Blood Proteins; C423236:CD274 protein, human; C498919:Cd274 protein, mouse; D008562:Membrane Glycoproteins; D010455:Peptides