title: Direct effects of cocaine-amphetamine-regulated transcript (CART) on pituitary hormone release in pituitary cell culture.
abstract: OBJECTIVE
  Cocaine- and amphetamine-regulated transcript (CART) is widely expressed in the rat brain, especially in the hypothalamic nuclei and in the anterior pituitary. The aim of this study was to evaluate the effects of CART on pituitary hormone release in pituitary cell culture.
  
  
  METHODS
  The pituitary hormone release from pituitary cell culture after CART administration was investigated. Concentrations of LH, FSH, PRL, TSH and GH were measured with RIA methods.
  
  
  RESULTS
  CART in all doses (1 nMol, 10 nMol, 100 nMol) stimulated prolactin (PRL) release and inhibited TSH release. CART administration caused a dose dependent decrease in LH release. CART did not change GH release from cultured pituitary cells.
  
  
  CONCLUSIONS
  CART may affect directly pituitary hormones release in the cell culture.
authors.affiliation: Neuroendocrinology Dept. Medical Centre of Postgraduate Education Warsaw, Poland. zncmkp@polbox.com
authors.forename: Boguslawa
authors.initials: B
authors.lastname: Baranowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Woliñska-Witort
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmielowska
authors.forename: Lidia
authors.initials: L
authors.lastname: Martyñska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Baranowska-Bik
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011921:Rats, Inbred WKY; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D010907:Pituitary Hormones; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone