title: Identification of neurabin II as a novel doublecortin interacting protein.
abstract: The neuronal migration protein doublecortin (DCX) that associates with microtubules through a tandem DCX repeat, is required for the development of the complex architecture of the human cerebral cortex. Using a yeast two-hybrid screen with Dcx as bait, we have isolated neurabin II/spinophilin, an F-actin binding protein known to play a role in dendritic spine formation. The coiled-coil domain of neurabin II binds to a DCX region encompassing the C-terminal portion of the second DCX repeat and the N-terminal portion of the Ser/Pro-rich domain. Immunoprecipitation experiments with brain extracts show that neurabin II and Dcx interact in vivo. Several Dcx constructs that mimic human DCX mutant alleles failed to interact with neurabin II. Since Dcx and neurabin II colocalized in the developing and adult brain, a neurabin II-DCX heterodimer may be involved in neuronal migration and dendritic spine formation.
authors.affiliation: Max Planck Institute for Experimental Endocrinology, Feodor-Lynen-Strasse 7, Hannover 30625, Germany.
authors.forename: Miki
authors.initials: M
authors.lastname: Tsukada
authors.forename: Alexander
authors.initials: A
authors.lastname: Prokscha
authors.forename: Judit
authors.initials: J
authors.lastname: Oldekamp
authors.forename: Gregor
authors.initials: G
authors.lastname: Eichele
mesh_terms: D000483:Alleles; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D002465:Cell Movement; D003712:Dendrites / Q000378:metabolism; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020080:Tandem Repeat Sequences; D013687:Telencephalon / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D000099273:Neurabins
chemical_list: D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011994:Recombinant Proteins; D000099273:Neurabins; C000714328:DCX protein, human; C000714847:Dcx protein, mouse