title: Correlation of death modes of photosensitized cells with intracellular ATP concentration.
abstract: The impact of intensity of glycolysis and oxidative phosphorylation on death of photosensitized murine hepatoma MH22 cells in vitro has been investigated. Cells photosensitized with meso-tetra(4-sulfonatophenyl)-porphine localized to lysosomes died mostly by necrosis, and the mode of cell death did not depend on the energy metabolism. Photosensitization with 5-aminolevulinic acid-stimulated endogenous porphyrins localized mainly in mitochondria or 5,10,15,20-tetrakis(m-hydroxyphenyl)-chlorine localized to cell membranes, including mitochondria, led to cell death mostly by apoptosis. In this case, the mode of cell death depended on the medium: under conditions unfavorable to glycolysis the ratio apoptosis/necrosis decreased significantly.
authors.affiliation: Department of Biochemistry and Biophysics, Vilnius University, Lithuania. vida.kirveliene@gf.vu.lt
authors.forename: Vida
authors.initials: V
authors.lastname: Kirveliene
authors.forename: Ausra
authors.initials: A
authors.lastname: Sadauskaite
authors.forename: Jurgis
authors.initials: J
authors.lastname: Kadziauskas
authors.forename: Sofija
authors.initials: S
authors.lastname: Sasnauskiene
authors.forename: Benediktas
authors.initials: B
authors.lastname: Juodka
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000494:pharmacology*; D000622:Aminolevulinic Acid / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D006019:Glycolysis / Q000187:drug effects / Q000528:radiation effects; D007424:Intracellular Fluid / Q000378:metabolism*; D008027:Light; D008247:Lysosomes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009336:Necrosis; D010085:Oxidative Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D010787:Photosensitivity Disorders / Q000139:chemically induced*; D011166:Porphyrins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D011166:Porphyrins; C016136:tetraphenylporphine sulfonate; D000622:Aminolevulinic Acid; D000255:Adenosine Triphosphate