[Inhibiting effect of ethanol extract from Achyranthes bidentata on A beta 42 aggregation].
OBJECTIVE
To observe inhibiting effect of ethanol extract from Achyranthes bidentata (Nx-E) on A beta 42 on (beta-amyloid protein 42) aggregation and fibril formation.


METHODS
A beta 42 and Nx-E were incubated in 37 degrees C, 5%, CO2 incubator first, fibril formation was monitored by thioflavine-T fluorescence and was observed with transmission electron microscopy.


RESULTS
At 7 days, 1 microgram.microL-1 A beta 42 fluorescence intensity was significantly increased, and much amyloid-like fibril was observed. A beta 42 co-incubated with 15 and 30 micrograms.microL-1 Nx-E for 7 days, their fluorescence intensities were remarkably reduced, few fibrils but an amorphous material were often observed.


CONCLUSIONS
After 1 microgram.microL-1 A beta 42 was incubated alone up to 7 days, A beta 42 aggregated and amyloid-like fibril formed. 15 and 30 micrograms.microL-1 Nx-E can prevent A beta 42 from polymerizing to some extent.
Luo
Huanmin
H
Pharmacy College, Jinan University, Guangzhou 510632.
Gu
Feng
F
Li
Xiaoguang
X
D029044:Achyranthes / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D010446:Peptide Fragments / Q000378:metabolism* / Q000648:ultrastructure; D010946:Plants, Medicinal / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D000431:Ethanol