Brassinosteroids promote root growth in Arabidopsis.
Although brassinosteroids (BRs) are known to regulate shoot growth, their role in the regulation of root growth is less clear. We show that low concentrations of BRs such as 24-epicastasterone and 24-epibrassinolide promote root elongation in Arabidopsis wild-type plants up to 50% and in BR-deficient mutants such as dwf1-6 (cbb1) and cbb3 (which is allelic to cpd) up to 150%. The growth-stimulating effect of exogenous BRs is not reduced by the auxin transport inhibitor 2,3,5-triidobenzoic acid. BR-deficient mutants show normal gravitropism, and 2,3,5-triidobenzoic acid or higher concentrations of 2,4-dichlorophenoxyacetic acid and naphtaleneacetic acid inhibit root growth in the mutants to the same extent as in wild-type plants. Simultaneous administration of 24-epibrassinolide and 2,4-dichlorophenoxyacetic acid results in largely additive effects. Exogenous gibberellins do not promote root elongation in the BR-deficient mutants, and the sensitivity to the ethylene precursor 1-aminocyclopropane-1-carboxylic acid is not altered. Thus, the root growth-stimulating effect of BRs appears to be largely independent of auxin and gibberellin action. Furthermore, we analyzed BR interactions with other phytohormones on the gene expression level. Only a limited set of auxin- and ethylene-related genes showed altered expression levels. Genes related to other phytohormones barely showed changes, providing further evidence for an autonomous stimulatory effect of BR on root growth.
Müssig
Carsten
C
Universität Potsdam, Institut für Biochemie und Biologie, Genetik, c/o Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany. muessig@mpimp-golm.mpg.de
Shin
Ga-Hee
GH
Altmann
Thomas
T
D000598:Amino Acids, Cyclic / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D004347:Drug Interactions / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
D000598:Amino Acids, Cyclic; D060406:Brassinosteroids; D002777:Cholestanols; D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; C023863:1-aminocyclopropane-1-carboxylic acid; C083545:castasterone; C023623:brassinolide