title: ONYX-015 enhances radiation-induced death of human anaplastic thyroid carcinoma cells.
abstract: ONYX-015 is a genetically modified adenovirus with a deletion of the E1B early gene and therefore is designed to replicate preferentially in p53-mutated cells causing their death. We previously demonstrated that the ONYX-015 virus kills anaplastic thyroid carcinoma (ATC) cells and enhances the antineoplastic effects of doxorubicin and paclitaxel. Here we report that ONYX-015 increased the cytopathic effect of radiotherapy in three ATC cell lines. In fact, ONYX-015 and radiation induced a significant cytopathic effect on ATC cells. DNA fragmentation analysis showed that ATC ONYX-015-treated cells were very sensitive to radiation-induced apoptosis. In addition, low doses of ONYX-015 associated with a single radiation dose of 10 Gy delayed the growth of a xenograft tumor induced by ARO cells in athymic mice. Our results suggest that the combination of ONYX-015 and radiotherapy should be considered for experimental trials in patients with anaplastic thyroid carcinoma.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Naples, Italy. portella@unina.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Portella
authors.forename: Roberto
authors.initials: R
authors.lastname: Pacelli
authors.forename: Silvana
authors.initials: S
authors.lastname: Libertini
authors.forename: Laura
authors.initials: L
authors.lastname: Cella
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Vecchio
authors.forename: Marco
authors.initials: M
authors.lastname: Salvatore
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D002277:Carcinoma / Q000532:radiotherapy*; D016923:Cell Death / Q000528:radiation effects; D003131:Combined Modality Therapy; D053938:DNA Fragmentation / Q000528:radiation effects; D051379:Mice; D008819:Mice, Nude; D011836:Radiation Tolerance; D013964:Thyroid Neoplasms / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014765:Viral Vaccines / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D014765:Viral Vaccines; C464532:dl1520