title: DNA conjugates as novel functional oligonucleotides.
abstract: Oligodeoxynucleotides with RNA cleavage activity 1) were conjugated with amines and peptides by solid phase fragment condensation (SPFC). It was found that 29 mer DNA enzyme conjugated with spermine at its 5'-end showed higher affinity to the target RNA sequence and 40 times higher activity of cleavage than native DNA enzyme. It is also to be noted that conjugate DNA enzymes showed increased resistance against nuclease digestion.
authors.affiliation: Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, Iizuka, Fukuoka, Japan. takanorikinki@hotmail.com
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Kinuko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Ryoji
authors.initials: R
authors.lastname: Ueki
authors.forename: Mayuka
authors.initials: M
authors.lastname: Yano
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Anno
authors.forename: Kotomi
authors.initials: K
authors.lastname: Sasaki
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013096:Spermine
chemical_list: D009838:Oligodeoxyribonucleotides; D013096:Spermine; D004247:DNA