Precursor-Directed polyketide biosynthesis in Escherichia coli.
Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.
Kinoshita
Kenji
K
Department of Chemistry, Box H, Brown University, Providence, RI 02912-9108, USA.
Pfeifer
Blaine A
BA
Khosla
Chaitan
C
Cane
David E
DE
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011498:Protein Precursors / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes; D011498:Protein Precursors