Flow cytometry of plant cells with applications in large-scale bioprocessing.
In recent years, there has been a significant upsurge in the application of flow cytometry to plant cells and plant cell cultures. As well as a range of uses in plant biology, flow cytometry offers many advantages for monitoring plant cell cultures used in large-scale bioprocessing operations. This review summarizes the current status of the field, concentrating on methods for DNA measurement and multiparameter cell cycle analysis. Techniques for screening and selection of elite cell lines with high productivity of secondary metabolites are also addressed.
Yanpaisan
W
W
Department of Biotechnology, University of New South Wales, Sydney NSW 2052, Australia.
King
N J
NJ
Doran
P M
PM