[Phenotype characteristic of the ionomycin-resistant CD4+ subset of the peripheral blood T-lymphocytes].
The differential sensitivity of peripheral blood (PB) CD4+ T lymphocytes to the calcium ionophore ionomycin was investigated. Effect of ionomycin exerted on T cells was time- and dose-dependent. We have shown that resistant cells belonged to some distinct T cell subsets. The resting naive CD4+CD45RA+ T cells showed a little, if any, resistance to ionomycin treatment. The primed CD4+CD45R0+ memory T cells behaved similarly as did ionomycin-resistant (IR) cells. Although IR CD4+ T cells had a typical "memory" phenotype, some quantitative differences were found in expression of CD11a, CD28, CD29, CD62L and CD243 markers between PB CD4+CD45R0+ T cells and corresponding IR cells.
Khaĭdukov
S V
SV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow. khsv@mail.ibch.ru
Kholodenko
I V
IV
Litvinov
I S
IS
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D016130:Immunophenotyping; D015759:Ionomycin / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
D015703:Antigens, CD; D015415:Biomarkers; D007476:Ionophores; D015759:Ionomycin; D002118:Calcium