title: Study of uranium transfer across the blood-brain barrier.
abstract: Uranium is a heavy metal which, following accidental exposure, may potentially be deposited in human tissues and target organs, the kidneys and bones. A few published studies have described the distribution of this element after chronic exposure and one of them has demonstrated an accumulation in the brain. In the present study, using inductively coupled plasma mass spectrometry (ICP-MS) for the quantification of uranium, uranium transfer across the blood-brain barrier (BBB) has been assessed using the in situ brain perfusion technique in the rat. For this purpose, a physiological buffered bicarbonate saline at pH 7.4 containing natural uranium at a given concentration was perfused. After checking the integrity of the BBB during the perfusion, the background measurement of uranium in control rats without uranium in the perfusate was determined. The quantity of uranium in the exposed rat hemisphere, which appeared to be significantly higher than that in the control rats, was measured. Finally, the possible transfer of the perfused uranium not only in the vascular space but also in the brain parenchyma is discussed.
authors.affiliation: INSERM-U26, Hôpital Fernand Widal, 75475 Paris Cedex 10, France. valerie.lemercier@voila.fr
authors.forename: V
authors.initials: V
authors.lastname: Lemercier
authors.forename: X
authors.initials: X
authors.lastname: Millot
authors.forename: E
authors.initials: E
authors.lastname: Ansoborlo
authors.forename: F
authors.initials: F
authors.lastname: Ménétrier
authors.forename: A
authors.initials: A
authors.lastname: Flüry-Hérard
authors.forename: Ch
authors.initials: Ch
authors.lastname: Rousselle
authors.forename: J M
authors.initials: JM
authors.lastname: Scherrmann
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000098:blood supply* / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000502:physiology; D007269:Injections, Intra-Arterial; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014501:Uranium / Q000008:administration & dosage* / Q000493:pharmacokinetics*
chemical_list: D014501:Uranium