title: Material-dependent levels of heat-shock protein 70 (hsp70) in human plasma following contact of blood with artificial surfaces.
abstract: Recently, it has been shown that heat-shock protein 70 (hsp70) functions in a dual role as a chaperone and a cytokine. However, no information is available on the occurrence of hsp70 in the extracellular milieu or on its ability to modulate cellular immune response. This study shows a material-dependent increase of hsp70 levels in plasma following contact of fresh heparinized whole human blood with three different biomaterials (PVC, heparin-coated PVC, Silicone). We report a previously unknown behavior of hsp70 to act as a plasma-adsorption protein. Further, high binding capacities for hsp70 to artificial surfaces (measured by Western blotting) and elevated hsp70 levels in plasma (measured by EIA) following contact with blood correspond with a reduced hemocompatibility. The degree of surface-induced activation of blood was determined by analysis of markers for coagulation, inflammation and complement activation. These findings indicate that the selective adsorption of hsp70 on artificial surfaces and the increased hsp70 levels in plasma may be important in directing host inflammatory and immune responses. We suggest that the levels of hsp70 in human plasma may represent a new prognostic factor or a diagnostic biomarker in hemostasis research.
authors.affiliation: Clinic for Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, Hoppe-Seyler-Street 3, 72076 Tuebingen, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Weber
authors.forename: Janina
authors.initials: J
authors.lastname: Caliebe
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ziemer
authors.forename: Hans P
authors.initials: HP
authors.lastname: Wendel
mesh_terms: D000327:Adsorption; D015415:Biomarkers / Q000097:blood; D001769:Blood / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D003176:Complement C3 / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006461:Hemolysis; D006487:Hemostasis; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D007378:Interleukins / Q000097:blood; D009000:Monocytes / Q000378:metabolism; D011143:Polyvinyl Chloride / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D003176:Complement C3; D018840:HSP70 Heat-Shock Proteins; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D001620:beta-Thromboglobulin; D005340:Fibrinogen; D011143:Polyvinyl Chloride; D006493:Heparin; D012825:Silicon