Effects of minerals on resistance of Bacillus subtilis spores to heat and hydrostatic pressure.
Among Bacillus subtilis IFO13722 spores sporulated at 30, 37, and 44 degrees C, those sporulated at 30 degrees C had the highest resistance to treatments with high hydrostatic pressure (100 to 300 MPa, 55 degrees C, 30 min). Pressure resistance increased after demineralization of the spores and decreased after remineralization of the spores with Ca(2+) or Mg(2+), whereas the resistance did not change when spores were remineralized with Mn(2+) or K(+), suggesting that former two divalent ions were involved in the activation of cortex-lytic enzymes during germination.
Igura
Noriyuki
N
Laboratory of Food Process Engineering, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka City, 8128581 Japan. igura@agr.kyushu-u.ac.jp
Kamimura
Yuya
Y
Islam
M Shahidul
MS
Shimoda
Mitsuya
M
Hayakawa
Isao
I
D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008903:Minerals / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
D003470:Culture Media; D008903:Minerals; D008345:Manganese; D008274:Magnesium; D011188:Potassium; D002118:Calcium