Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci.
Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.
Paule
Suzanne M
SM
Northwestern Memorial Hospital, Northwestern University, Chicago, Illinois, USA. spaule@enh.org
Trick
William E
WE
Tenover
Fred C
FC
Lankford
Mary
M
Cunningham
Susan
S
Stosor
Valentina
V
Cordell
Ralph L
RL
Peterson
Lance R
LR
D001426:Bacterial Proteins / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D041981:Gastrointestinal Tract / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020713:Vancomycin Resistance
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases