title: Screening of exogenous enzymes for ruminant diets: relationship between biochemical characteristics and in vitro ruminal degradation.
abstract: With the objective of developing a rational approach for the selection of feed enzymes for ruminants, 22 commercial enzyme products were examined in terms of protein concentration, enzymic activities on model substrates, and hydrolytic capacity, the latter determined from the release of reducing sugars from alfalfa hay and corn silage. An in vitro ruminal degradation assessment was carried out using the same substrates, untreated or treated with the 22 enzyme products at 1.5 microL/g forage DM. Stepwise regressions were then performed to establish relationships between these factors. Protein concentration and enzymic activities explained at least 84% (P < 0.01) of the variation in the release of reducing sugars from alfalfa and corn silage. Alfalfa DM degradation after incubation with ruminal fluid for 18 h was positively related to xylanase activity (R2 = 0.29, P < 0.01), but the same activity was negatively related to DM degradation of corn silage (R2 = 0.19, P < 0.05). Protease activity explained a further 10% of the alfalfa DM degradation (P < 0.10). Following sequential steps involving the determination of rate and extent of DM and fiber degradation, the best candidates for alfalfa and corn silage were selected. Enzyme products effective with alfalfa hay seemed to exert part of their effect during the pretreatment period, whereas enzymes effective with corn silage worked exclusively after ruminal fluid was added. This finding suggests that different modes of action of exogenous enzymes are attacking different substrates and may partly explain enzyme-feed specificity. In alfalfa, it seems that effective enzymes work by removing structural barriers that retard the microbial colonization of digestible fractions, increasing the rate of degradation. In corn silage, effective enzymes seem to interact with ruminal enzymes to degrade the forage more rapidly, which is consistent with previous findings of synergism between exogenous and ruminal enzymes.
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge, AB, Canada T1J 4B1. colombat@agro.uba.ar
authors.forename: D
authors.initials: D
authors.lastname: Colombatto
authors.forename: D P
authors.initials: DP
authors.lastname: Morgavi
authors.forename: A F
authors.initials: AF
authors.lastname: Furtado
authors.forename: K A
authors.initials: KA
authors.lastname: Beauchemin
mesh_terms: D000821:Animal Feed; D000818:Animals; D004043:Dietary Fiber / Q000378:metabolism*; D004798:Enzymes / Q000494:pharmacology*; D006868:Hydrolysis; D066298:In Vitro Techniques; D000455:Medicago sativa; D011506:Proteins / Q000378:metabolism*; D012044:Regression Analysis; D012417:Rumen / Q000378:metabolism*; D012418:Ruminants; D012820:Silage; D013379:Substrate Specificity; D003313:Zea mays
chemical_list: D004043:Dietary Fiber; D004798:Enzymes; D011506:Proteins