title: Purification, crystallization and preliminary X-ray studies of a p-nitrophenylphosphatase from Bacillus stearothermophilus.
abstract: Thermostable p-nitrophenylphosphatase from Bacillus stearothermophilus has been expressed in Escherichia coli, purified and crystallized. The crystals belong to space group C(2), with unit-cell parameters a = 67.17 A, b = 57.84 A, c = 62.49 A and alpha = 90.0 degrees, beta = 95.4 degrees, gamma = 90.0 degrees. Diffraction data were collected to 1.40 A resolution with a completeness of 94.7% (96.6% for the last shell), an R(fac) value of 0.074 (0.341) and an I/sigma (I) value of 30.1 (2.67).
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, 200433, People's Republic of China.
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Ji
authors.forename: Liang
authors.initials: L
authors.lastname: Tian
authors.forename: Cong-Jing
authors.initials: CJ
authors.lastname: Feng
authors.forename: Gang
authors.initials: G
authors.lastname: Yin
authors.forename: Guang
authors.initials: G
authors.lastname: Shu
authors.forename: Ji-Xi
authors.initials: JX
authors.lastname: Li
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Gong
authors.forename: Hai
authors.initials: H
authors.lastname: Pang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Mao
mesh_terms: D009597:4-Nitrophenylphosphatase / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000706:statistics & numerical data; D001411:Geobacillus stearothermophilus / Q000201:enzymology*
chemical_list: D009597:4-Nitrophenylphosphatase