Development of an animal-human antibody complex for use as a control in ELISA.
In order to provide the equivalent of a human anti-human protein antibody as positive control in ELISAs, a goat-human antibody complex was created using chemical cross-linking. The resulting hybrid complex had a larger molecular size on HPLC and SDS-PAGE. In ELISA, the goat-human complex bound to human antigen and was detectable by a secondary anti-human conjugate. The method to make the hybrid complex is simple, cost-effective and can be used to make human-like antibodies to many human proteins.
Gu
Mi Li
ML
BioAnalytical Sciences Development Department, Human Genome Sciences Inc., Rockville, MD 20850, USA. mili_gu@hgsi.com
Feng
S L
SL
Glenn
J K
JK
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D012709:Serum Albumin / Q000276:immunology
D000906:Antibodies; D003432:Cross-Linking Reagents; D007136:Immunoglobulins; D012709:Serum Albumin