Characterization of a salicylic acid- and pathogen-induced lipase-like gene in Chinese cabbage.
A cDNA clone for a salicylic acid-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene, designated Br-sil1 (for Brassica rapa salicylate-induced lipase-like 1 gene), encodes a putative lipase that has the family II lipase motif GDSxxDxG around the active site serine. A database search showed that plant genomes have a large number of genes that contain the family II lipase motif. The lipase-like proteins include a myrosinase-associated protein, an anther-specific proline-rich protein APG, a pollen coat protein EXL, and an early nodule-specific protein. The Br-sil1 gene is strongly induced by salicylic acid and a nonhost pathogen, Pseudomonas syringae pv. tomato, that elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with BTH, methyl jasmonate, or ethephon showed that the Br-sil1 gene expression is induced by BTH, but not by methyl jasmonate or ethylene. This indicates that the cabbage gene is activated via a salicylic acid-dependent signaling pathway. An examination of the tissue-specific expression revealed that the induction of the Br-sil1 gene expression by BTH occurs in leaves and stems, but not in roots and flowers. Without the BTH treatment, however, the Br-sil1 gene is not expressed in any of the tissues that were examined.
Lee
Kyung-Ah
KA
Division of Life Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 360-763, Korea.
Cho
Tae-Ju
TJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D029741:Brassica rapa / Q000201:enzymology / Q000235:genetics*; D030541:Databases, Genetic; D005656:Fungal Proteins; D020869:Gene Expression Profiling; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020156:Salicylic Acid / Q000378:metabolism*
D005656:Fungal Proteins; D008049:Lipase; C104055:lipase II; D020156:Salicylic Acid