title: Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease.
abstract: By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.
authors.affiliation: Unit of Experimental Neuroscience, Department of Clinical Neuroscience, Göteborg University, Sahlgrenska University Hospital/Mölndal, S-431 80 Mölndal, Göteborg, Sweden. maja.puchades@neuro.gu.se
authors.forename: Maja
authors.initials: M
authors.lastname: Puchades
authors.forename: Sara Folkesson
authors.initials: SF
authors.lastname: Hansson
authors.forename: Carol L
authors.initials: CL
authors.lastname: Nilsson
authors.forename: Niels
authors.initials: N
authors.lastname: Andreasen
authors.forename: Kaj
authors.initials: K
authors.lastname: Blennow
authors.forename: Pia
authors.initials: P
authors.lastname: Davidsson
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D001053:Apolipoproteins / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006023:Glycoproteins / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D040901:Proteomics / Q000379:methods*; D012016:Reference Values
chemical_list: D001053:Apolipoproteins; D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D006023:Glycoproteins