Specific oligobodies against human brain acetylcholinesterase.
In order to develop the specific oligobodies against human brain acetylcholinesterase (AChE) and distinguishes between human erythrocyte and brain AChEs, we applied the strategy of 'target switching' to obtain the specific polyclonal and monoclonal oligobodies. The specificity between human brain AChE and other ChEs was identified by Western blotting, dot blotting and enzyme protein binding assay (EPBA). The results showed that the oligobodies against the human brain AChE specifically immunoreacted with the human brain AChE and Torpedo AChE, not showing significant binding to AChE from human erythrocyte and butyrylcholinesterase (BChE) from human serum.
Zhang
Xing Mei
XM
Beijing Institute of Pharmacology and Toxicology, Beijin 100850, China.
Li
Qian
Q
Shi
Yu Sheng
YS
Wu
Jun Hua
JH
Shao
Ning Sheng
NS
Liu
Gang
G
Sun
Man Ji
MJ
D000110:Acetylcholinesterase / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002802:Cholinesterases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D011869:Radioligand Assay; D014101:Torpedo
D000906:Antibodies; D019151:Peptide Library; D010455:Peptides; D000110:Acetylcholinesterase; D002802:Cholinesterases