Polymerase chain reaction (PCR) techniques for site-directed mutagenesis.
Polymerase chain reaction (PCR) technology has revolutionized the process of isolating and amplifying segments of DNA. One powerful application of PCR is its use in precise site-directed mutagenesis (SDM). SDM provides an elegant tool for scientists and engineers to explore biocatalytic mechanisms and processes to understand the structural-functional relationships of enzymes and other proteins. This article reviews techniques and methodology used in site-directed mutagenesis of genes by PCR.
Reikofski
J
J
Biochemical and Food Process Engineering, Department of Agricultural Engineering, Purdue University, West Lafayette, IN, USA.
Tao
B Y
BY