Consecutive analysis of bacterial PCR samples on a single electronic microarray.
For routine mass screening, the use of microarrays is hampered because one chip can only analyze one sample. 16S rRNA gene PCR products of several bacterial strains or mixtures thereof were consecutively loaded on a single electronic microarray and successfully analyzed using probes specific for the bacterial strains.
Zimmermann
Klaus
K
Baxter BioScience, Industriestrasse 72, A-1221, Vienna, Austria. zimmerk@baxter.com
Eiter
Thomas
T
Scheiflinger
Friedrich
F
D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D036103:Nanotechnology / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S