title: Occurrence of a specific protein in basidiomycete-lytic enzyme preparation produced by Bacillus circulans KA-304 inductively with a cell-wall preparation of Schizophyllum commune.
abstract: KA-prep, a culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation (CWP) of Schizophyllum commune, has been reported to have an activity to form protoplasts from S. commune mycelia. The SDS-polyacrylamide gel electrophoreses described here demonstrated that a specific proteinous component (molecular weight: 150,000) occurred in KA-prep. The protein (P150T) was also formed in culture filtrates with CWP of several basidiomycetes, which could release the protoplasts, suggesting that the component was an indispensable factor for protoplast formation. P150T, isolated from an ammonium sulfate fraction of KA-prep (0-30% saturation), did not have any protoplast-forming activity. Results were obtained indicating that P150T participates in protoplast formation together with chitinase(s) and beta-glucanase(s) in KA-prep. The N-terminal amino acid sequence indicated an analogy of P150T to mutanase (alpha-1,3-glucanase) from Bacillus sp. RM1, and actually P150T hydrolyzed mutan as well as S-(alpha-1,3) glucan from S. commune.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Science and Technology, Ritsumeikan University, Shiga, Japan.
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Yano
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toge
authors.forename: Mamoru
authors.initials: M
authors.lastname: Wakayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Tachiki
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D003470:Culture Media / Q000737:chemistry; D004790:Enzyme Induction / Q000187:drug effects; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011523:Protoplasts / Q000378:metabolism; D012567:Schizophyllum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D005936:Glucans; D006026:Glycoside Hydrolases