Discrimination of peptides by using a molecularly imprinted piezoelectric biosensor.
Based on the direct formation of a molecularly imprinted polymer on gold electrodes, we have developed a peptide sensor for the detection of low-molecular-weight peptides. A new cross-linking monomer, (N-Acr-L-Cys-NHBn)(2), was employed to attach the surface of the chip and to copolymerize with other monomers. Interestingly, N-benzylacrylamide participates in the polymerization and recognition is carried out in an aqueous environment. By using quartz crystal microbalance detection, short peptides can be monitored by their interaction with plastic antibodies specific for the target peptides. The selectivity of molecularly imprinted polymers and the sensitivity of such artificial biosensors have been combined to differentiate between traces of oxytocin and vasopressin to the ng mL(-1) scale.
Lin
Chung-Yin
CY
Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan.
Tai
Dar-Fu
DF
Wu
Tzong-Zeng
TZ
D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D003432:Cross-Linking Reagents / Q000737:chemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010121:Oxytocin / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011791:Quartz / Q000737:chemistry; D014667:Vasopressins / Q000032:analysis
D003432:Cross-Linking Reagents; D010455:Peptides; D014667:Vasopressins; D011791:Quartz; D010121:Oxytocin; D006046:Gold