Synthesis and energy transfer efficiency of FRET terminators derived from different linkers.
A number of different energy transfer dye labeled-cassettes were synthesized using aminoacid based trifunctional linkers and coupled to the propargylamino-substituted dideoxynucleoside-5'-triphosphates (ddNTPs). These terminators were evaluated for their energy transfer efficiency and DNA sequencing potential using thermostable DNA polymerase.
Kumar
Shiv
S
Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA. shiv.kumar@amersham.com
Nampalli
Satyam
S
Finn
Patrick J
PJ
Sudhakar Rao
T
T
Chen
Chung-Yuan
CY
Flick
Parke K
PK
Fuller
Carl W
CW
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D013728:Terminator Regions, Genetic
D005456:Fluorescent Dyes; D004247:DNA; D004259:DNA-Directed DNA Polymerase