title: N-3 fatty acid-rich diet prevents early response of interleukin-6 elevation in trinitrobenzene sulfonic acid-induced enteritis.
abstract: Dietary fat is an important factor involved in the pathogenesis of inflammatory bowel disease (IBD). It remains unclear how n-3 and n-6 fatty acids modulate intestinal inflammation. In this study, we investigated the effects of n-3 and n-6 fatty acid-rich diets on trinitrobenzene sulfonic acid (TNBS)-induced enteritis. The rats were fed an n-3 or n-6 rich-diet for 12 days, and then starved for the following 2 days. An intraileal injection of TNBS was administered, and TNBS-enteritis subsequently developed. Macroscopic and histological examination was performed after 24 h. Serum cytokine levels were determined by ELISA. The n-6 fatty acid-rich diet markedly enhanced mucosal damage as compared to the n-3 fatty acid-rich diet. The damage score was significantly higher in the n-6 fatty acid-rich diet group (P<0.05). Histological changes in the mucosa were more severe in the n-6 fatty acid-rich diet group than in the n-3 fatty acid-rich group. Serum IL-6 levels were significantly higher in the n-6 fatty acid-rich diet group than in the n-3 fatty acid-rich group (P<0.05). On the other hand, there were no differences in serum TNF-alpha levels. The n-3 fatty acid-rich diet effectively reduced early mucosal inflammation in TNBS enteritis. The effects of the n-3 fatty acids were associated with blockage of mucosal IL-6 secretion. Our data suggest that n-3 fatty acid-rich diet may be applicable for enteral nutrition in the treatment of IBD patients.
authors.affiliation: Department of Internal Medicine, Shiga University of Medical Science, Seta-Tukinowa, Otsu 520-2192, Japan. andoh@belle.shiga-med.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Andoh
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tsujikawa
authors.forename: Izumi
authors.initials: I
authors.lastname: Ishizuka
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Araki
authors.forename: Masaya
authors.initials: M
authors.lastname: Sasaki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Koyama
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
mesh_terms: D000821:Animal Feed; D000818:Animals; D004032:Diet; D018450:Disease Progression; D004751:Enteritis / Q000097:blood* / Q000139:chemically induced / Q000178:diet therapy* / Q000473:pathology; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000139:chemically induced / Q000178:diet therapy / Q000473:pathology; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*; D017962:alpha-Linolenic Acid / Q000008:administration & dosage* / Q000627:therapeutic use*
chemical_list: D015850:Interleukin-6; D017962:alpha-Linolenic Acid; D014302:Trinitrobenzenesulfonic Acid