title: Development of a conductimetric biosensor using immobilised Rhodococcus ruber whole cells for the detection and quantification of acrylonitrile.
abstract: A conductimetric biosensor for the detection of acrylonitrile in solution was designed and characterised using whole cells of Rhodococcus ruber NCIMB 40757, which were immobilised into a disc of dimethyl silicone sponge (ImmobaSil). The biosensor described was capable of the detection and quantification of acrylonitrile in aqueous solution, having a linear response to concentrations between 2 and 50 mM (106-2650 ppm) acrylonitrile. The biosensor has been shown to be reproducible with respect to the data obtained over a number of days, and retains stability for a minimum period of at least 5 days before recalibration of the biosensor is required.
authors.affiliation: Biotechnology Centre, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, West Yorkshire, HD1 3DH, UK. p.c.j.roach@hud.ac.uk
authors.forename: P C J
authors.initials: PC
authors.lastname: Roach
authors.forename: D K
authors.initials: DK
authors.lastname: Ramsden
authors.forename: J
authors.initials: J
authors.lastname: Hughes
authors.forename: P
authors.initials: P
authors.lastname: Williams
mesh_terms: D000181:Acrylonitrile / Q000032:analysis* / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018914:Cells, Immobilized / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012240:Rhodococcus / Q000187:drug effects* / Q000502:physiology*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology*
chemical_list: D014874:Water Pollutants, Chemical; D000181:Acrylonitrile