title: Angiotensin converting enzyme inhibitory phenylpropanoid glycosides from Clerodendron trichotomum.
abstract: The stems of Clerodendron trichotomum have been traditionally used for treatment of hypertension in far East Asia including China, Korea, and Japan. Bioassay-guided fractionation and purification of the EtOAc-soluble extract of Clerodendron trichotomum afforded acteoside (1), leucosceptoside A (2), martynoside (3), acteoside isomer (4), and isomartynoside (5). The angiotensin converting enzyme (ACE) activities were significantly inhibited by the addition of these phenylpropanoid glycosides (1-5) in a dose-dependent manner of which IC(50) values were 373+/-9.3 microg/ml, 423+/-18.8 microg/ml, 524+/-28.1 microg/ml, 376+/-15.6 microg/ml, 505+/-26.7 microg/ml, respectively. These results suggest that the antihypertensive effect of Clerodendron trichotomum may be, at least in part, due to ACE inhibitory effect of phenylpropanoid glycosides.
authors.affiliation: Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeon-Buk 570-749, South Korea.
authors.forename: Dae Gill
authors.initials: DG
authors.lastname: Kang
authors.forename: Yong Sup
authors.initials: YS
authors.lastname: Lee
authors.forename: Hyoung Ja
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Ho Sub
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000085:Acetates; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D032409:Clerodendrum; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems; D010946:Plants, Medicinal; D011422:Propionates / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
chemical_list: D000085:Acetates; D000806:Angiotensin-Converting Enzyme Inhibitors; D006027:Glycosides; D010936:Plant Extracts; D011422:Propionates; C007650:ethyl acetate