Malformation of immature starfish oocytes by theonellapeptolide Ie, a Tridecapeptide lactone from a marine sponge Petrosia species, through disturbance of cortical F-actin distribution.
Theonellapeptolide Ie (Tp), an oligopeptide lactone isolated from a marine sponge, Petrosia sp., was shown to induce an unprecedented morphological change in the immature oocytes of the starfish Asterina pectinifera. The cortical F-actin was disturbed and assembled to form dots and rings, as evidenced by staining with rhodamine-conjugated phalloidin. The oocyte eventually became malformed. When Tp was added to an immature oocyte which had been pretreated with cytochalasin B or D, inhibitors of actin polymerization, no malformation was observed. When Tp was added to an oocyte which had been induced to mature by 1-methyladenine (1-MeAde), a maturation-inducing substance in starfishes, no morphological changes were observed in the maturing oocytes which reached the first meiotic prometaphase 40 min after the start of 1-MeAde treatment. This is the first description of a chemical that induces aberrant redistribution of F-actin-based cytoskeleton in an animal oocyte which is arrested at the first meiotic prophase.
Ohta
Emi
E
Laboratory of Molecular Cell Biology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Okada
Hironobu
H
Ohta
Shinji
S
Kobayashi
Motomasa
M
Kitagawa
Isao
I
Horiike
Shintaro
S
Takahashi
Tadao
T
Hosoya
Hiroshi
H
Yamamoto
Kenya
K
Ikegami
Susumu
S
D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D015638:Cytochalasin D / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D008853:Microscopy / Q000379:methods; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000633:toxicity*; D011161:Porifera / Q000737:chemistry*; D011494:Protein Kinases / Q000378:metabolism; D013215:Starfish / Q000502:physiology*; D013997:Time Factors
D000199:Actins; D006133:Growth Substances; D010455:Peptides; C053945:theonellapeptolide Ie; D015638:Cytochalasin D; C008407:1-methyladenine; D011494:Protein Kinases; C102333:histone H1 kinase; D000225:Adenine