New reagent for protein-DNA contacts footprinting.
We have found, that the reaction of o-bromobenzoic acid with Cu2+ ions can be used as a source of activated oxygen species capable of cleaving DNA. Possibility to apply this reaction for footprinting the nucleosome core in the reconstituted chromatin was demonstrated.
Koval
O A
OA
Laboratory of Nucleic Acids Biochemistry, Institute of Bioorganic Chemistry, Novosibirsk, Russia. o.koval@niboch.nsc.ru
Oleinikova
S B
SB
Chernolovskaya
E L
EL
Litvak
V V
VV
Vlassov
V V
VV
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting / Q000379:methods*; D006868:Hydrolysis; D007202:Indicators and Reagents; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000737:chemistry*
D007202:Indicators and Reagents; D009707:Nucleosomes; D011506:Proteins; D004247:DNA