A new dimension of 1H-NMR spectroscopy in assessment of liver graft dysfunction.
High-resolution 1H-NMR spectroscopy of serum and urine samples of an 11-year-old male living related orthotopic liver transplant recipient is reported. Serum glutamine increased to abnormal levels along with simultaneous abnormal excretion of urinary glutamine post-transplantation. High levels of glutamine in both blood and urine and concomitant reduced urea levels in urine were found to be evidence of impairment in urea cycle and compatible with persistently abnormal graft function. Thus glutamine levels in serum and urine, and urea in the urine as observed by 1H-NMR spectroscopy highlight their important roles in monitoring liver graft function; increased glutamine levels lead to brain damage, if untreated.
Singh
H K
HK
Department of Radiodiagnosis, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226 014, India. hksingh@sgpgi.ac.in
Yachha
S K
SK
Saxena
Rajan
R
Gupta
Ashish
A
Nagana Gowda
G A
GA
Bhandari
M
M
Khetrapal
C L
CL
D000328:Adult; D000818:Animals; D002648:Child; D005260:Female; D005973:Glutamine / Q000097:blood / Q000652:urine; D006084:Graft Rejection / Q000097:blood* / Q000175:diagnosis / Q000652:urine*; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D014508:Urea / Q000652:urine
D005973:Glutamine; D014508:Urea