title: Proteome analysis identified maspin as a special feature of papillary thyroid carcinoma.
abstract: This study aimed at investigating new mechanisms of carcinogenesis in thyroid cancer at the molecular level and at finding potential protein markers involved in the initiation of the different histological subtypes. For this, we performed differential proteome analysis on primary cultured thyrocytes (PT) and transformed thyrocytes (TT) derived from 238Pu alpha-particle irradiation using 2-dimensional electrophoresis (2-DE) and peptide mass fingerprinting (PMF) with matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS). Image analysis showed that one protein was very strongly expressed in TT; 55 proteins were weaker, different in intensity, including 26 spots that were increased in PT, and 29 spots were decreased. The hot spot was identified as maspin, a unique member of the serpin family considered to be a class II tumor suppressor gene. To clarify the role of maspin in thyroid carcinogenesis we searched for protein expression in 20 normal (tumor-free) tissues, as well as in 20 follicular adenomas (FAD), 20 papillary carcinomas (PTC), 20 follicular carcinomas (FTC), 20 poorly differentiated carcinomas (PDTC), and 20 undifferentiated carcinomas (UTC). Maspin protein expression was detectable in none of the cases of normal tumor-free thyroid tissue, nor in FAD, FTC, PDTC and UTC. In contrast 14 of 20 PTC (70%) showed a moderate or strong cytoplasmic staining; 4 of these 14 cases had a moderate cytoplasmic and nuclear staining. In conclusion, we hypothesize that maspin protein expression is a special feature in the cascade of PTC genesis and that the way of initiating PTC is different from other thyroid carcinoma types.
authors.affiliation: Department of Pathology, Otto-von-Guericke University, Magdeburg, Germany. carsten.boltze@medizin.uni-magdeburg.de
authors.forename: Carsten
authors.initials: C
authors.lastname: Boltze
authors.forename: Regine
authors.initials: R
authors.lastname: Schneider-Stock
authors.forename: Claudia
authors.initials: C
authors.lastname: Quednow
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gerlach
authors.forename: Christian
authors.initials: C
authors.lastname: Mawrin
authors.forename: Raoul
authors.initials: R
authors.lastname: Hinze
authors.forename: Albert
authors.initials: A
authors.lastname: Roessner
authors.forename: Cuong
authors.initials: C
authors.lastname: Hoang-Vu
mesh_terms: D002291:Carcinoma, Papillary / Q000175:diagnosis / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D019992:Databases as Topic; D015180:Electrophoresis, Gel, Two-Dimensional; D016147:Genes, Tumor Suppressor; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D014176:Protein Biosynthesis; D011506:Proteins; D020543:Proteome; D015843:Serpins / Q000096:biosynthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D011506:Proteins; D020543:Proteome; C085344:SERPIN-B5; D015843:Serpins