Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing.
Fluorescence resonance energy transfer (FRET) dye labeled cassettes and terminators with one or more donor dyes (fluorescein) and acceptor dye (rhodamine dyes) with benzofuran or tyrosine linker moieties were synthesized. These terminators were evaluated for their energy transfer and DNA sequencing potential using thermostable DNA polymerase.
Sudhakar Rao
T
T
Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA.
Zhang
Weihong
W
Xiao
Haiguang
H
Flick
Parke
P
Kumar
Shiv
S
Nampalli
Satyam
S
D001483:Base Sequence; D001572:Benzofurans; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D013728:Terminator Regions, Genetic; D014443:Tyrosine
D001572:Benzofurans; D014443:Tyrosine; D004247:DNA; C105430:benzofuran