title: Potential of magnetic resonance lymphography with intrapulmonary injection of gadopentetate dimeglumine for visualization of the pulmonary lymphatic basin in dogs: preliminary results.
abstract: OBJECTIVE
  To evaluate a new approach of magnetic resonance (MR) lymphography with intraalveolar injection of a conventional extracellular contrast agent (gadopentetate dimeglumine) for imaging lymphatic basin draining from specific portions of the lung.
  
  
  METHODS
  Three-dimensional T1-weighted spoiled gradient-recalled echo MR sequence images were acquired serially before and for 40 minutes after intraalveolar injection of gadopentetate dimeglumine in a total of 14 anesthetized beagle dogs. Six of these dogs received 1 mL undiluted and low-concentration (75%) contrast agent into the same portion of the right caudal lobe during a 7-day interval. In all dogs, including these 6 dogs, MR lymphography was repeated with injection of the low-concentration contrast agent into different lung regions at 7-day intervals to evaluate the differences of the visualized draining lymphatic station. Lymphatic enhancement was quantified by percent increases of signal intensity against precontrast. Postmortem examination of the lymphatic anatomy was performed in 7 of these animals.
  
  
  RESULTS
  In all dogs, the lymphatic station draining from the injection sites was visualized within 5 minutes after contrast injection. The maximum percent increase of signal intensity of the same middle tracheobronchial lymph nodes was significantly greater with a low-concentration (75%) contrast agent than with an undiluted one in the same 6 dogs (n = 6, 247.6 +/- 30.5% vs. 204.2 +/- 33.8%; P < 0.01). Different lymphatic stations draining from the different injection sites were visualized in all dogs. In a total of 12 MR studies that showed extended nodal enhancement after injection of the low-concentration contrast agent, the enhancement peak of the most proximal nodes (n = 12) from the injection sites appeared earlier than that of their distant nodes (n = 12), with a maximum percent increase of signal intensity of 249.8 +/- 42.4%. The visualized lymph nodes were found in the appropriate locations postmortem, with significant correlation for nodal sizes (r = 0.965; P < 0.0001).
  
  
  CONCLUSIONS
  MR lymphography with low-concentration gadopentetate dimeglumine can quickly and sufficiently visualize the drainage lymphatic station from specific lung portions, and may have the potential of sentinel node mapping in lung cancer.
authors.affiliation: Department of Radiology, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Suga
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.forename: Ogasawara
authors.initials: O
authors.lastname: Nobuhiko
authors.forename: Munemasa
authors.initials: M
authors.lastname: Okada
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Naofumi
authors.initials: N
authors.lastname: Matsunaga
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D004285:Dogs; D005260:Female; D019786:Gadolinium DTPA / Q000008:administration & dosage; D007267:Injections; D008168:Lung / Q000033:anatomy & histology*; D008208:Lymphatic System / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging; D011650:Pulmonary Alveoli
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA