Activating transcription factor 3 mRNA is upregulated in primary cultures of trigeminal ganglion neurons.
Activating transcription factor 3 (ATF3) has been used in as a marker of nerve injury in primary sensory neurons. The purpose of the present studies was to determine whether primary sensory ganglia in culture express ATF3 and, thus, an injured phenotype. At all time points post-plating (1 h-14 days), neurons in culture expressed ATF3 compared to undetectable expression in native ganglia. In addition, NGF was unable to rescue this injured phenotype. Thus, sensory neurons in culture represent a potential model of injured neurons.
Dussor
Gregory O
GO
Department of Pharmacology, The University of Texas Health Science Center, San Antonio, TX 78229, USA.
Price
Theodore J
TJ
Flores
Christopher M
CM
D051700:Activating Transcription Factor 3; D000818:Animals; D002478:Cells, Cultured; D008297:Male; D008954:Models, Biological; D020932:Nerve Growth Factor / Q000494:pharmacology; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; D020932:Nerve Growth Factor