Endcaps for stabilizing short DNA duplexes.
The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
Ng
Pei-Sze
PS
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA.
Pingle
Maneesh R
MR
Balasundarum
Ganesan
G
Friedman
Alan
A
Zu
Xiaolin
X
Bergstrom
Donald E
DE
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013816:Thermodynamics
D004247:DNA