Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash.
A lily strain of Cucumber mosaic virus (LK-CMV) was not able to systemically infect zucchini squash (Cucurbita pepo), while Fny strain of CMV (Fny-CMV) caused systemic mosaic and stunting symptom at 4 days post-inoculation on the same host species. The pathogenicity of LK-CMV in zucchini squash was investigated by reassortments of genomic RNAs of LK-CMV and Fny-CMV for infection, as well as by pseudorecombinants generated from biologically active transcripts of cDNA clones of LK-CMV and Fny-CMV, respectively. The assessments of pathogenicity for LK-CMV indicated that RNA2 of LK-CMV was responsible for systemic infection in zucchini squash. In the protoplast of zucchini squash, the RNA accumulations of all constructed pseudorecombinants were indistinguishable and LK-CMV replication was slightly lower than that of Fny-CMV, suggesting that the inability of LK-CMV to infect squash plants was responsible for the poor efficiency of virus movement, rather than the reduction of replication function.
Choi
Seung Kook
SK
Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
Choi
Jang Kyung
JK
Ryu
Ki Hyun
KH
D018548:Cotyledon / Q000821:virology; D017799:Cucumovirus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D028464:Cucurbita / Q000821:virology*; D015723:Gene Library; D016679:Genome, Viral; D027762:Lilium / Q000821:virology*; D009068:Movement; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D012327:RNA Virus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D014779:Virus Replication / Q000235:genetics
D012367:RNA, Viral