title: Gene expression, cell localization, and evolution of rodent submandibular gland androgen-binding protein.
abstract: A small dimeric androgen-binding protein (Abp) secreted by mouse (Mus) submandibular salivary glands has been hypothesized to function in mate-selection. The alpha-subunit (Abpa) evolves rapidly under natural selection. However, cellular site(s) of synthesis, mode of function, and patterns of evolution of this biologically important protein are otherwise unknown. We used a radiolabeled riboprobe and in situ hybridization to mouse (Mus) Abpa mRNA to localize Abpa synthesis to submandibular gland acinar cells. We next used a quantitative fluorescent thermal cycler (real-time PCR) to determine relative expression levels (normalized with a constitutively expressed ribosomal gene [S15/rig; rat insulinoma gene]) in male, female, and sexually immature European striped field mice (Apodemus agrarius). We hypothesized that gender or age-related differences might occur in production of a salivary protein related to sexual selection, but found no significant differences within our sample. Finally, we also used reverse transcription-PCR of mRNAs isolated from submandibular salivary glands to determine the Abpa allele in the striped wood mouse and compare it to published information on the homologue in Mus musculus domesticus.
authors.affiliation: Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston 79409-3131, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Wickliffe
authors.forename: V H
authors.initials: VH
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Tandler
authors.forename: C J
authors.initials: CJ
authors.lastname: Phillips
mesh_terms: D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009115:Muridae; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism*
chemical_list: D000727:Androgen-Binding Protein; D018076:DNA, Complementary; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides