title: Efficiency of the hybrid capture 2 HPV DNA test in cervical cancer screening. A study by the French Society of Clinical Cytology.
abstract: The aim of this study was to determine the efficiency of the Hybrid Capture 2 (HC2; Digene, Gaithersburg, MD) human papillomavirus (HPV) assay for the detection of cervical neoplasia. Of the 1,785 patients recruited, 462 (25.88%) were referred for colposcopy owing to previously detected cytologic abnormalities, and 1,323 (74.12%) were voluntary candidates for screening. For all patients, a Papanicolaou smear and a monolayer smear (ThinPrep, Cytyc, Boxborough, MA) were done. HPV DNA was detected on the residual liquid-based material. False-positive results were observed in 111 cases and comprised 34 cross-reactions (1.90%) and 77 false-positive cases (4.31%) owing to a contiguous strong chemiluminescence signal. Interestingly, all these samples had a relative light units value of 1 to 3 and were contiguous to a sample with a very high HPV DNA load. The final results showed that high-risk and low-risk HPV DNA were detected in 480 samples (26.89%) and 135 samples (7.56%), respectively. Although HC2 can be considered a reliable and sensitive test for HPV DNA detection, we do not advocate its use for large-scale screening for cervical neoplasia.
authors.affiliation: Department of Tumor Biology, Institut Curie, Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: de Cremoux
authors.forename: Joël
authors.initials: J
authors.lastname: Coste
authors.forename: Xavier
authors.initials: X
authors.lastname: Sastre-Garau
authors.forename: Martine
authors.initials: M
authors.lastname: Thioux
authors.forename: Christelle
authors.initials: C
authors.lastname: Bouillac
authors.forename: Sylvain
authors.initials: S
authors.lastname: Labbé
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cartier
authors.forename: Marianne
authors.initials: M
authors.lastname: Ziol
authors.forename: Anne
authors.initials: A
authors.lastname: Dosda
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Galès
authors.forename: Vincent
authors.initials: V
authors.lastname: Molinié
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Vacher-Lavenu
authors.forename: Béatrix
authors.initials: B
authors.lastname: Cochand-Priollet
authors.forename: Philippe
authors.initials: P
authors.lastname: Vielh
authors.forename: Henri
authors.initials: H
authors.lastname: Magdelénat
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D003951:Diagnostic Errors; D005260:Female; D005602:France; D006801:Humans; D008403:Mass Screening / Q000191:economics / Q000379:methods*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012958:Societies, Scientific; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000517:prevention & control; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral