[Effect of epidural morphine analgesia on the NO-forming activity of nociceptive neurons of the spinal ganglia and bone marrow].
The effect of epidural morphine analgesia on NO mechanisms was studied during the primary and secondary sensitizations. The nitro-oxide synthase (NOS) activity was evaluated by the method of Home and Vincent, 1989, (identification of NADPH diaphorase). NOS and NADPH diaphorases are two double enzymes in cells with a synchronously changing activity. The NOS activity is in line with the level of the NO secretion. The enzyme was measured on a microdensitometer. The findings evidence an active involvement of NO mechanisms in the formation of primary and secondary hyperalgesia, and the injection of morphine caused a significant decrease in the count of NO-expressing neurons in the total number of cells with high and average activities of the enzyme.
Shumatov
V B
VB
Shumatova
T A
TA
Balashova
T V
TV
D000767:Anesthesia, Epidural; D001854:Bone Marrow Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D006930:Hyperalgesia / Q000201:enzymology / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D009569:Nitric Oxide; D009020:Morphine; D019001:Nitric Oxide Synthase