Expression of HSP70 in peripheral lymphocytes of the patients with allergic rhinitis.
The expression levels of heat shock protein 70 (HSP70) from peripheral lymphocytes of the patients with allergic rhinitis (AR) and the clinical implication were investigated. In the morning, 3 ml of fasting venous blood was taken out. The lymphocytes were isolated by using Ficoll-Hypaque and the expression of HSP70 in the lymphocytes was detected by using Western blot. In the AR patients the HSP70 level (41.49 +/- 15.77 integrated optical density, IOD) were significantly higher than that in the control group (23.89 +/- 10.13 IOD, P < 0.05). Western blot demonstrated that HSP70 bands in AR patients were more intensive than those in the control group. It was concluded that the elevated HSP70 level in peripheral lymphocytes of the AR patients might contribute to the development of AR.
Liu
Lisi
L
Department of Otorhinolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Xiao
Chengfeng
C
Zhang
Ming
M
Cheng
Lei
L
Wang
Efen
E
Wu
Tangchun
T
D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood*
D018840:HSP70 Heat-Shock Proteins