[The effect of ouabain on sensory neuron neurite growth in organotypic culture].
Ouabain, a known inhibitor of Na+, K(+)-ATP, taken in a wide range of concentrations, was investigated in organotypic tissue culture of dorsal root ganglia cells of 10-11 day old chick embryos. Ouabain inhibited neurite growth in a dose-dependent manner. The Hill coefficient was defined as 1, and the Kp value was estimated as 1 x 10(-10) M. At inhibitor concentrations exceeding 1 x 10(-9) M, the growth of neurites was totally inhibited. It is assumed that Na+, K(+)-ATPase may play an important role in regulation of the process of neurite growth in sensory neurones.
Panniiaĭnen
V A
VA
I. P. Pavlov Institute of Physiology RAS, St. Petersburg.
Krylov
B V
BV
Chalisova
N I
NI
Malevskaia
I I
II
D000818:Animals; D002455:Cell Division; D002642:Chick Embryo; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005724:Ganglia / Q000187:drug effects* / Q000196:embryology; D016501:Neurites / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D010042:Ouabain