title: Molecular follow up of donor lymphocyte infusion in CML children after allogeneic bone marrow transplantation.
abstract: In this study we present the applicability of fluorescence in situ hybridisation (FISH) and RT-PCR to detect the minimal residual disease (MRD) in relapsed Ph+ children after donor lymphocyte infusion (DLI) post bone marrow transplantation (BMT). In both patients BCR/ABL fusion was detected and its transcript at the moment of relapse. After the DLI treatment in short time intervals a decreasing number of cells with BCR/ABL fusion were noticed and the expression of the hybrid gene disappeared. These results demonstrate that all the methods presented in this study provide a feasible, rapid and accurate way for the detecting of the minimal residual disease after DLI in Ph positive CML patients.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Ładon
authors.forename: A
authors.initials: A
authors.lastname: Pieczonka
authors.forename: J
authors.initials: J
authors.lastname: Jółkowska
authors.forename: J
authors.initials: J
authors.lastname: Wachowiak
authors.forename: M
authors.initials: M
authors.lastname: Witt