title: Clonal expansion of double-positive intraepithelial lymphocytes by MHC class I-related chain A expressed in mouse small intestinal epithelium.
abstract: Expression of a distant homologue MHC class I molecule, MHC class I-related chain A (MICA), has been found to be stress inducible and limited to the intestinal epithelium. This nonclassical MHC molecule is associated with various carcinomas in humans. To understand the biological consequences of MICA expression in the gut, we generated transgenic (Tg) mice (T3(b)-MICA Tg) under the control of the T3(b) promoter. The T3(b)-MICA Tg mice expressed MICA selectively in the intestine and had an increased number of TCRalphabeta CD4CD8alphaalpha, double-positive (DP) intraepithelial lymphocytes (IELs) in the small bowel. These MICA-expanded DP IELs exhibited a bias to Vbeta8.2 and overlapped motifs of the complementarity-determining region 3 region among various Tg mice. Hence, the overexpression of MICA resulted in a clonal expansion of DP IELs. Studies in model of inflammatory bowel disease showed that transgenic MICA was able to attenuate the acute colitis induced by dextran sodium sulfate administration. Therefore, this unique in vivo model will enable investigation of possible influences of stress-inducible MICA on the gut immune surveillance.
authors.affiliation: Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Park
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takahashi
authors.forename: Junko
authors.initials: J
authors.lastname: Ikeda
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kawahara
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Okamoto
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kweon
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuyama
authors.forename: Veronika
authors.initials: V
authors.lastname: Groh
authors.forename: Thomas
authors.initials: T
authors.lastname: Spies
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Obata
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Miyazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002999:Clone Cells; D016264:Dextran Sulfate / Q000008:administration & dosage; D004195:Disease Models, Animal; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D043183:Irritable Bowel Syndrome / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis; D019076:Transgenes / Q000276:immunology / Q000502:physiology
chemical_list: D015703:Antigens, CD; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C113249:T cell receptor peptide Vbeta8.1; C028388:thymus-leukemia antigens; D016264:Dextran Sulfate