Media for hybridoma growth and monoclonal antibody production.
For the economical production of monoclonal antibodies (MAbs), the cell-culture medium must be optimized for three different phases: growth of the hybridomas, MAb productivity of the hybridomas, and MAb purification or downstream processing. Medium improvements are necessary to meet these requirements for large-scale MAb production. Information bearing on this issue is being addressed in two research areas, cell biology and biochemical engineering, and is reviewed in this article.
Bols
N C
NC
Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 2G1, Canada.
Scharer
J M
JM
Phillips
H A
HA
Moo-Young
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