Altered blood-brain barrier permeability and its effect on the distribution of Evans blue and sodium fluorescein in the rat brain applied by intracarotid injection.
The aim was to study the blood-brain permeability according to the distribution in the rat brain of Evans blue (EB) and sodium fluorescein (NaFl) administered by an intracarotid injection. Eighteen animals were divided into six groups according to the state of the blood-brain barrier (BBB) at the moment when the dyes were being applied. In the first two groups, the BBB was intact, in groups 3 and 4 the barrier had been opened osmotically prior to the application of the dyes, and in groups 5 and 6 a cellular edema was induced by hyperhydration before administration of the dyes. The intracellular and extracellular distribution of the dyes was studied by fluorescence microscopy. The histological picture thus represented the morphological correlate of the way BBB permeability had been changed before the application of the dyes.
Kozler
P
P
Neurosurgical Clinic, Central Military Hospital, U vojenské nemocnice 1200, 169 02 Prague 6, Czech Republic. nchr@uvn.cz
Pokorný
J
J
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000503:physiopathology*; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D001923:Brain Chemistry; D001929:Brain Edema / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D017536:Carotid Artery, Common; D002343:Carotid Artery, Internal; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D018891:Dentate Gyrus / Q000098:blood supply / Q000378:metabolism; D005070:Evans Blue / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019793:Fluorescein / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism; D007010:Hyponatremia / Q000503:physiopathology; D007269:Injections, Intra-Arterial; D007274:Injections, Intraperitoneal; D008353:Mannitol / Q000008:administration & dosage / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009997:Osmotic Pressure; D051381:Rats; D017208:Rats, Wistar; D014867:Water / Q000008:administration & dosage / Q000494:pharmacology
D014867:Water; D008353:Mannitol; D005070:Evans Blue; D019793:Fluorescein