title: PNA-DNA chimeras forming quadruplex structures.
abstract: 1H-NMR, CD, and UV spectroscopy have been used to investigate the structure of PNA/DNA chimeras forming quadruplex structures. In particular, we synthesized 5'TGGG3'-t (1) and 5'TGG3'-gt (2), where lower and upper case letters indicate PNA and DNA residues, respectively. CD spectrum and all NMR data of (1) are typical of quadruplexes involving four parallel strands. UV melting profile of (1) indicates that its thermal stability is quite similar to that observed for the reference structure [d(TGGGT)]4. 1H-NMR spectrum for 5'TGG3'-gt (2) shows that this oligonucleotide is not able to fold into a single, well-defined species.
authors.affiliation: Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Esposito
authors.forename: Aldo
authors.initials: A
authors.lastname: Galeone
authors.forename: Luciano
authors.initials: L
authors.lastname: Mayol
authors.forename: Anna
authors.initials: A
authors.lastname: Messere
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
authors.forename: Antonio
authors.initials: A
authors.lastname: Randazzo
mesh_terms: D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D004247:DNA