Fluorescence studies on nyctinasty which suggest the existence of genus-specific receptors for leaf-movement factor.
Periodic leaf-movement of legumes is called nyctinasty and has been known since the age of Alexander the Great. We found that nyctinasty is controlled by a periodic change of the internal concentration of leaf-opening and leaf-closing substances in the plant body. Now, we have developed novel fluorescent probes (1) based on the structure of cis-p-coumaroylagmatine (3), which was isolated as a leaf-opening substance of Albizzia juribrissin Durazz. Binding experiments using probe 1 showed that Albizza plants have receptors for a leaf-opening substance in their motor cells. By using probes 1 we then found that genus-specific receptors are involved in nyctinasty.
Nagano
Hideharu
H
Laboratory of Natural Products Chemistry, Department of Chemistry, Faculty of Science and Technology, Keio University, Hiyoshi, Yokohama 223-8522, Japan.
Kato
Eisuke
E
Yamamura
Shosuke
S
Ueda
Minoru
M
D001681:Biological Assay; D002625:Chemistry, Organic / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D031298:Mimosa / Q000737:chemistry* / Q000502:physiology*; D008956:Models, Chemical; D009068:Movement; D018515:Plant Leaves / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D010946:Plants, Medicinal; D011485:Protein Binding
D005456:Fluorescent Dyes