[Analysis of kojic acid in Aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography].
A new and an efficient method for analyzing kojic acid in aspergillus oryzae ferment by ion-pair reversed-phase high performance liquid chromatography was developed. The kojic acid was well separated on a C18 column using methanol-0.01 mol/L disodium hydrogen phosphate and 2 mmol tetrabutylammonium bromide (4:96) as the mobile phase, followed by an UV detection at 226 nm. The detection limit was 0.012 microgram/ml. The spiked recoveries in wort-yeast culture, wort-peptone culture and potato-yeast-sugar culture were 98.2%, 103.4% and 97.2%, respectively. The RSD values were 0.51%, 0.45% and 0.43% respectively.
Zhao
Shan
S
Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Li
Yepeng
Y
Zhao
Haiyan
H
Ji
Rong
R
Li
Yuwei
Y
D001236:Aspergillus oryzae / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media; D011753:Pyrones / Q000032:analysis*
D003470:Culture Media; D011753:Pyrones; C011890:kojic acid