title: Pro-inflammatory effects of Burkholderia cepacia on cystic fibrosis respiratory epithelium.
abstract: Burkholderia cepacia causes pulmonary infection with high mortality in cystic fibrosis (CF) patients which is likely to involve interaction with respiratory epithelium. In this study the pro-inflammatory properties of B. cepacia were examined using a range of respiratory epithelial cell lines. B. cepacia and cell-free culture supernatants were used to stimulate cell lines with (SigmaCFTE29o- and IB3) and without (A549) the CF transmembrane conductance regulator mutation (CFTR), together with corrected cell lines (C38 and S9). Interleukin (IL)-6 and IL-8, but not GM-CSF or IL-1beta, were released from all the cell lines whereas PGE(2) (prostaglandin E(2)) was released from the A549, IB3 and S9 cell lines only. Nuclear factor (NF)-kappaB activation preceded cytokine release and suppression of NF-kappaB activity diminished cytokine release. These studies indicated that B. cepacia secretory products are potent pro-inflammatory agents for respiratory epithelium and suggest functional CFTR is not required for cytokine or prostanoid responses.
authors.affiliation: School of Biomedical and Chemical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.
authors.forename: Joshua
authors.initials: J
authors.lastname: Fink
authors.forename: James H
authors.initials: JH
authors.lastname: Steer
authors.forename: David A
authors.initials: DA
authors.lastname: Joyce
authors.forename: Andrew S
authors.initials: AS
authors.lastname: McWilliam
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Stewart
mesh_terms: D016956:Burkholderia cepacia / Q000276:immunology / Q000472:pathogenicity*; D002460:Cell Line; D045744:Cell Line, Tumor; D003550:Cystic Fibrosis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000502:physiology; D016207:Cytokines / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D015533:Transcriptional Activation
chemical_list: C505032:CFTR protein, human; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone