CHIP activates HSF1 and confers protection against apoptosis and cellular stress.
Induction of molecular chaperones is the characteristic protective response to environmental stress, and is regulated by a transcriptional program that depends on heat shock factor 1 (HSF1), which is normally under negative regulatory control by molecular chaperones Hsp70 and Hsp90. In metazoan species, the chaperone system also provides protection against apoptosis. We demonstrate that the dual function co-chaperone/ubiquitin ligase CHIP (C-terminus of Hsp70-interacting protein) regulates activation of the stress-chaperone response through induced trimerization and transcriptional activation of HSF1, and is required for protection against stress-induced apoptosis in murine fibroblasts. The consequences of this function are demonstrated by the phenotype of mice lacking CHIP, which develop normally but are temperature-sensitive and develop apoptosis in multiple organs after environmental challenge. CHIP exerts a central and unique role in tuning the response to stress at multiple levels by regulation of protein quality control and transcriptional activation of stress response signaling.
Dai
Qian
Q
Carolina Cardiovascular Biology Center, and Department of Medicine, University of North Carolina, Chapel Hill, NC, USA.
Zhang
Chunlian
C
Wu
Yaxu
Y
McDonough
Holly
H
Whaley
Ryan A
RA
Godfrey
Virginia
V
Li
Hui-Hua
HH
Madamanchi
Nageswara
N
Xu
Wanping
W
Neckers
Len
L
Cyr
Douglas
D
Patterson
Cam
C
D019943:Amino Acid Substitution; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D000076249:Heat Shock Transcription Factors; D006358:Hot Temperature; D051379:Mice; D018345:Mice, Knockout; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D014157:Transcription Factors; D015533:Transcriptional Activation; D044767:Ubiquitin-Protein Ligases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases