Specific inhibition of hepatitis C viral gene expression by non-polar (phenylalkyl)phosphonates.
Different phenylalkyl backbone modified antisense oligonucleotides complementary to the Hepatitis C virus (HCV) RNA nucleotides 326-342 were synthesized. The lipohilic character of modified oligonucleotides was determined from RP-HPLC retention times. The inhibitory effect of these antisense oligonucleotides on HCV gene expression was analyzed in an in vitro test system.
Amberg
S
S
Institute of Organic Chemistry and Chemical Biology, J.W. Goethe University, Frankfurt/Main, Germany. s.amberg@chemie.uni.frankfurt.de
Tamke
A
A
Caselmann
W H
WH
Engels
J W
JW
D020137:Base Pair Mismatch / Q000187:drug effects; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D016174:Hepacivirus / Q000187:drug effects / Q000235:genetics*; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis / Q000494:pharmacology*; D063065:Organophosphonates / Q000494:pharmacology*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020319:Oligodeoxyribonucleotides, Antisense; D063065:Organophosphonates