Fosmidomycin resistance in adenylate cyclase deficient (cya) mutants of Escherichia coli.
Adenylate cyclase deficient (cya) mutants of E. coli K-12 were found to be resistant to fosmidomycin, a specific inhibitor of the non-mevalonate pathway, just like to fosfomycin. E. coli glpT mutants were resistant to fosfomycin and also to fosmidomycin. This fact shows that fosmidomycin was transported inside via the glycerol-3-phosphate transporter, GlpT. DNA micro-array analysis showed that the transcription of glpT and other genes concerning glycerol utilization were highly dependent on the presence of cAMP.
Sakamoto
Yoshiko
Y
Department of Food Science and Technology, College of Bioresouce Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Furukawa
Soichi
S
Ogihara
Hirokazu
H
Yamasaki
Makari
M
D000262:Adenylyl Cyclases / Q000172:deficiency / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D005578:Fosfomycin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005838:Genotype; D008826:Microbial Sensitivity Tests; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis
D000900:Anti-Bacterial Agents; D005578:Fosfomycin; C024640:fosmidomycin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases