Synthesis, biophysical, and biochemical properties of PNA-DNA chimeras.
Three PNA-DNA chimeric dimer synthons (tT, upT and uhT, see Sch. 1) have been synthesized in solution and used to make T20-analogue chimeras applying standard solid-phase DNA synthesis protocol. Duplex forming ability of chimeras with dA20 and their hydrolyses by 3'- and 5'-exonucleases (snake venom and bovine spleen phosphodiesterase, respectively) have been investigated.
Bajor
Zoltán
Z
Chemical Research Center, Institute of Chemistry, Hungarian Academy of Sciences, Budapest, Hungary. bajor@chemres.hu
Sági
Gyula
G
Tegyey
Zsuzsanna
Z
Otvös
László
L
D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D019281:Dimerization; D007202:Indicators and Reagents; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D004247:DNA