Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.
Somatic embryogenesis from leaf explants of Scaevola aemula R. Br. was achieved. Somatic embryos were induced from explants cultured on MS medium supplemented with 0.2 mg/ 2,4-dichlorophenoxyacetic acid and 0.2-0.5 mg/l 6-benzylaminopurine (BAP). Various developmental stages of somatic embryos were found on this medium-from globular embryos to germinated embryos. The transfer of globular embryos to MS medium containing 0.5 mg/l BAP resulted in a high frequency of shoot regeneration. Leaf explants cultured on MS medium containing different combinations of BAP and alpha-naphthaleneacetic acid formed adventitious shoots and roots. Histological examination confirmed the process of somatic embryogenesis. Induction of somatic embryogenesis in Scaevola provides a system for studying embryogenesis in Australian native plants and will facilitate the improvement of these plants using genetic transformation techniques.
Wang
Y-H
YH
Plant Molecular Biology and Biotechnology Laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia.
Bhalla
P L
PL
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019659:Asteraceae / Q000166:cytology / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development*; D001593:Benzyl Compounds; D002454:Cell Differentiation; D003624:Darkness; D035264:Flowers / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000254:growth & development; D007701:Kinetin; D008027:Light; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000254:growth & development; D011687:Purines; D012038:Regeneration
D001593:Benzyl Compounds; D010937:Plant Growth Regulators; D011687:Purines; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin