Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species.
A novel method, based on PCR Restriction Fragment Length Polymorphism Analysis (PRA) of a part of the tuf gene (370 bp), was designed for the identification of 11 staphylococcal species, including the most common staphylococcal pathogens. A total of 258 clinical isolates were validated by this assay, and the results were in concordance with those obtained by the reference method of Kloos and Schleifer.
Kontos
F
F
Department of Microbiology, School of Medicine, University of Thessalia, Papakyriazi 22, Larissa, Greece.
Petinaki
E
E
Spiliopoulou
I
I
Maniati
M
M
Maniatis
A N
AN
D000465:Algorithms; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D010444:Peptide Elongation Factor Tu / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial; D010444:Peptide Elongation Factor Tu