[Study on the effect of the growth and aflatoxin production by Aspergillus flavus parasiticus NRRL 2999 in the present of Lactobacillus plantarum ATCC 8014].
The effect of L. plantarum ATCC 8014 on growth and aflatoxin B1 production of A. flavus subsp. parasiticus NRRL 2999 was studied and determined. A spore suspension of Aspergillus flavus parasiticus NRRL 2999 is inoculated into MRS medium containing L. plantarum and incubated at 28 degrees C for 15 days. The pH of the medium, weight of mycelia and aflatoxin B1 production were determined on day 3, 6, 9, 12, 15 of incubation. The growth of A. flavus subsp. parasiticus NRRL 2999 was inhibited when spores were added to a 24 h L. plantarum ATCC8014 culture and when both organisms were added simultaneously. The inhibition of the growth of A. flavus subsp. parasiticus NRRL 2999 was probably due to inactivating the viability of spores. The spores of A. flavus subsp. parasiticus NRRL 2999 became swollen in the presence of L. plantarum ATCC8014 culture, at the same time, and A. flavus subsp. parasiticus NRRL 2999 also affected the shape of L. plantarum ATCC8014 cells, which became enlarged. However, when L. plantarum ATCC8014 was added to a 3-day A. flavus subsp. parasiticus NRRL 2999 culture, there was not effect on mold growth and aflatoxin B1 production. Lactic acid inhibited growth of A. flavus subsp. culture in the MRS medium with initial pH value of 4.5. However, the production of aflatoxin B1 was not affected.
Xu
Jin
J
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Wang
Huijun
H
Ji
Rong
R
Luo
Xueyun
X
D016604:Aflatoxin B1 / Q000032:analysis / Q000096:biosynthesis*; D001231:Aspergillus flavus / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000502:physiology*; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D016604:Aflatoxin B1