Binding studies of eukaryotic initiation factor eIF4E with novel mRNA dinucleotide cap analogues.
Studies on the interaction of the murine translation initiation factor 4E with two new-synthesized cap-analogues, modified at C2' of 7-methylguanosine, have been performed by means of the fluorescence titration method. No difference in the binding affinity for eIF4E was observed compared with the "anti reversed" cap analogues, possessing the analogous modifications at C3'. Potential significance of the novel caps as research tools for examination of the nuclear cap binding complex CBC80/20 has been discussed.
Zuberek
Joanna
J
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland.
Jemielity
Jacek
J
Stepinski
Janusz
J
Lewdorowicz
Magdalena
M
Niedzwiecka
Anna
A
Haber
Dorota
D
Stolarski
Ryszard
R
Rhoads
Robert E
RE
Darzynkiewicz
Edward
E
D000818:Animals; D001665:Binding Sites; D015226:Dinucleoside Phosphates; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship
D015226:Dinucleoside Phosphates; D039561:Eukaryotic Initiation Factor-4E; D012315:RNA Caps; D012333:RNA, Messenger; C062229:7-methyl-diguanosine triphosphate