Different susceptibility of human mesothelial cells to polyomavirus infection and malignant transformation.
SV40 has been associated with mesothelioma development. The possible role of the closely related human polyomaviruses JC virus (JCV) and BK virus (BKV) in mesothelioma remained unclear. We found that JCV did not infect human mesothelial cells. BKV and SV40 infected mesothelial cells, expressed viral oncoproteins, and caused similar alterations of key cell regulatory genes. BKV replicated faster than SV40 and caused mesothelial cell lysis, not cellular transformation. SV40 did not lyse mesothelial cells and caused a high rate of transformation. These findings provide a rationale for the observation that SV40 is found in mesothelioma, rather than the ubiquitous human JCV and BKV.
Carbone
Michele
M
Cardinal Bernardin Cancer Center, Department of Pathology, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA.
Burck
Carol
C
Rdzanek
Monica
M
Rudzinski
Jennifer
J
Cutrone
Rochelle
R
Bocchetta
Maurizio
M
D000818:Animals; D001739:BK Virus / Q000502:physiology*; D002472:Cell Transformation, Viral / Q000502:physiology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004198:Disease Susceptibility; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D005260:Female; D006801:Humans; D007577:JC Virus / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D008654:Mesothelioma / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D010766:Phosphorylation; D027601:Polyomavirus Infections / Q000378:metabolism / Q000821:virology; D011518:Proto-Oncogene Proteins; D019859:Proto-Oncogene Proteins c-met; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D013539:Simian virus 40 / Q000502:physiology*; D019098:Telomerase / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D014412:Tumor Virus Infections / Q000378:metabolism / Q000821:virology; D014779:Virus Replication
D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D011518:Proto-Oncogene Proteins; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D015534:Trans-Activators; D014157:Transcription Factors; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met; D019098:Telomerase