title: Degranulation and superoxide production depend on cholesterol in PLB-985 cells.
abstract: The effect of agents disrupting cholesterol-rich microdomains of the cell membrane was studied on the chemoattractant receptor (FPR and FRPL1) coupled effector responses of promyelocytic PLB-985 cells. Both methyl-beta-cyclodextrin (MbetaCD) and filipin III inhibited exocytosis of primary granules and O(2)(.-) production induced by stimulation of either chemotactic receptor. Alteration of calcium homeostasis of MbetaCD-treated cells does not account for the impairment of the effector responses. Disruption of microfilaments by cytochalasin B (CB) partially reverses the inhibitory effect of cholesterol depletion. Our results provide functional support for the involvement of cholesterol-rich membrane domains in the signaling of chemotactic receptors and call the attention to the possible role of microfilaments in the organization of lipid microdomains.
authors.affiliation: Department of Physiology, Semmelweis University, POB 259, H-1444 Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Káldi
authors.forename: Agnes
authors.initials: A
authors.lastname: Kalocsai
authors.forename: Balázs K
authors.initials: BK
authors.lastname: Rada
authors.forename: Gábor
authors.initials: G
authors.lastname: Mezo
authors.forename: Gergely Z
authors.initials: GZ
authors.lastname: Molnár
authors.forename: György
authors.initials: G
authors.lastname: Báthori
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Ligeti
mesh_terms: D000595:Amino Acid Sequence; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D003505:Cyclodextrins / Q000494:pharmacology; D005372:Filipin / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D013481:Superoxides; D005372:Filipin; D002784:Cholesterol