Detection of melon necrotic spot virus in water samples and melon plants by molecular methods.
Melon necrotic spot virus (MNSV) is a water and soil-borne pathogen affecting species of the Cucurbitaceae family both in hydroponic and soil crops. Molecular methods for detecting MNSV in water samples, nutrient solutions and melon plants were developed. For this purpose, water samples from a water source pool of a hydroponic culture or from the recirculating nutrient solution were concentrated by ultracentrifugation or PEG precipitation followed by RT-PCR analysis. Both concentration methods were suitable to allow the detection of MNSV and represent, as far as we know, the first time that this virus has been detected in water samples. A non-isotopic riboprobe specific for MNSV was obtained and used to detect the virus in plant tissue. Different parts of mechanically infected plants were examined including the roots, stems, inoculated cotyledons and young leaves. Excluding the inoculated cotyledons, the tissues showing the highest accumulation levels of the virus were the roots. The potential inclusion of such tools in management programs is discussed.
Gosalvez
B
B
Centro de Edafología y Biología Aplicada del Segura (CEBAS), CSIC, Campus Universitario de Espinardo, Murcia 30100, Spain.
Navarro
J A
JA
Lorca
A
A
Botella
F
F
Sánchez-Pina
M A
MA
Pallas
V
V
D000914:Antibodies, Viral / Q000032:analysis; D019184:Carmovirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D028462:Cucumis / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases / Q000821:virology*; D018514:Plant Structures / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014871:Water Microbiology
D000914:Antibodies, Viral; D018076:DNA, Complementary; D012367:RNA, Viral