Monocyte chemoattractant protein-1 (MCP-1) and macrophage infiltration into the skin after burn injury in aged mice.
Clinical observations and laboratory studies have shown a delay in dermal wound healing in aged subjects. Since macrophages play a key role in wound healing, we investigated age related differences in MCP-1 production and monocyte recruitment to the wound following burn injury using a murine model. The present study shows that there is an increase in MCP-1 levels in the burned-normal skin interface at 1-day post burn in both young and aged burned mice compared to sham injured mice. However, the levels of MCP-1 in aged burned mice (133.16+/-36.55pg/mg protein) were approximately half the levels of young burned mice (286.15+/-45.36pg/mg protein, P<0.05). Additionally, at 4 days post burn, MCP-1 levels in aged mice (290.73+/-101.98) reached the same levels as in young mice (243.97+/-36.71). There was no difference in macrophage accumulation into the wound between young and aged at either time point. These data demonstrate that the difference in dermal MCP-1 levels between the young and aged is not associated with a difference in macrophage infiltration to the wound following burn injury, suggesting that the lower MCP-1 content in the aged is possibly affecting other phases of wound healing in the aged.
Shallo
Hilda
H
Department of Cell Biology, 2160 South First Avenue, Stritch School of Medicine, Loyola University Chicago, 60513, Maywood, IL, USA.
Plackett
Timothy P
TP
Heinrich
Scott A
SA
Kovacs
Elizabeth J
EJ
D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000503:physiopathology; D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology
D018932:Chemokine CCL2