Occurrence of the allene oxide cyclase in different organs and tissues of Arabidopsis thaliana.
Occurrence of an essential enzyme in jasmonate (JA) biosynthesis, the allene oxide cyclase, (AOC) was analyzed in different developmental stages and various organs of Arabidopsis thaliana plants by immuno blot analysis and immunocytological approaches. Levels of AOC and of the two preceding enzymes in JA biosynthesis increased during seedling development accompanied by increased levels of JA and 12-oxophytodienoic acid levels after 4 and 8 weeks. Most tissues including all vascular bundles and that of flower buds contain AOC protein. Flowers shortly before opening, however, contain AOC protein preferentially in ovules, stigma cells and vascular bundles, whereas in anthers and pollen AOC could not be detected. The putative roles of AOC and JA in development are discussed.
Hause
Bettina
B
Institute of Plant Biochemistry, Department of Secondary Metabolism, Weinberg 3, D-06120 Halle/S., Germany.
Stenzel
Irene
I
Miersch
Otto
O
Wasternack
Claus
C
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D015152:Blotting, Northern; D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000378:metabolism; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008084:Lipoxygenase / Q000032:analysis / Q000378:metabolism; D054883:Oxylipins; D018514:Plant Structures / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014018:Tissue Distribution
D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D008084:Lipoxygenase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase