Microwave sterilization of femoral head allograft.
The potential shortage of allograft bone has led to the need to investigate other sources of bone for allografts. Some allograft bone donated from primary total hip arthroplasty recipients must be discarded or treated to become usable as a result of bacterial contamination. Femoral head allografts were contaminated with Staphylococcus aureus and Bacillus subtilis. A domestic microwave oven was used. The contaminated bone was exposed to microwave irradiation for different time periods. The samples were then cultured to attempt to grow the two bacterial species. The contaminated bone samples failed to grow any organisms after 2 min of exposure to microwave irradiation. This study shows that sterilization of femoral head allografts contaminated with S. aureus and B. subtilis can be achieved with microwave irradiation in a domestic microwave oven. This method of sterilization of bone allografts is cheap, easily used, and an effective way to process contaminated bone.
Dunsmuir
Robert A
RA
Department of Orthopaedics, Southern General Hospital, Glasgow, United Kingdom. Robert.dunsmuir@leedsth.nhs.uk
Gallacher
Grace
G
D001412:Bacillus subtilis / Q000254:growth & development / Q000528:radiation effects; D018586:Bone Banks; D001847:Bone Diseases / Q000382:microbiology; D005270:Femur Head / Q000382:microbiology / Q000528:radiation effects / Q000637:transplantation*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008872:Microwaves; D013211:Staphylococcus aureus / Q000254:growth & development / Q000528:radiation effects; D013242:Sterilization / Q000379:methods*; D014184:Transplantation, Homologous