Identification of bacteria recovered from clinical specimens by 16S rRNA gene sequencing.
The sequence of the 16S rRNA gene has been used extensively for phylogenetic classification, identification, and genotypic typing of bacteria. Identification of bacterial isolates by 16S rRNA gene sequencing, though generally performed in reference laboratories, has been recently introduced for routine use in clinical laboratories to identify isolates that cannot be identified by conventional methods. Described in this report is the use of 16S rRNA gene sequencing to identify uncommon bacteria, or bacteria with unusual phenotypic properties, with four brief case presentations to illustrate its clinical application. The feasibility, usefulness and limitations of performing this approach in the clinical laboratory are also discussed.
Kiratisin
P
P
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, 10700 Bangkoknoi, Bangkok, Thailand.
Li
L
L
Murray
P R
PR
Fischer
S H
SH
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D005240:Feasibility Studies; D005243:Feces / Q000382:microbiology; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013048:Specimen Handling; D013785:Thailand; D014947:Wounds and Injuries / Q000382:microbiology
D012336:RNA, Ribosomal, 16S