Substance P presynaptically depresses the transmission of sensory input to bronchopulmonary neurons in the guinea pig nucleus tractus solitarii.
Substance P modulates the reflex regulation of respiratory function by its actions both peripherally and in the CNS, particularly in the nucleus tractus solitarii (NTS), the first central site for synaptic contact of the lung and airway afferent fibres. There is considerable evidence that the actions of substance P in the NTS augment respiratory reflex output, but the precise effects on synaptic transmission have not yet been determined. Therefore, we determined the effects of substance P on synaptic transmission at the first central synapses by using whole-cell voltage clamping in an NTS slice preparation. Studies were performed on second-order neurons in the slice anatomically identified as receiving monosynaptic input from sensory nerves in the lungs and airways. This was done by the fluorescent labelling of terminal boutons after 1,1'-dioctadecyl-3,3,3',3'-tetra-methylindocarbo-cyanine perchlorate (DiI) was applied via tracheal instillation. Substance P (1.0, 0.3 and 0.1 microM) significantly decreased the amplitude of excitatory postsynaptic currents (eEPSCs) evoked by stimulation of the tractus solitarius, in a concentration-dependent manner. The decrease was accompanied by an increase in the paired-pulse ratio of two consecutive eEPSCs, and a decrease in the frequency, but not the amplitude, of spontaneous EPSCs and miniature EPSCs, findings consistent with a presynaptic site of action. The effects were consistently and significantly attenuated by a neurokinin-1 (NK1) receptor antagonist (SR140333, 3 muM). The data suggest a new site of action for substance P in the NTS (NK1 receptors on the central terminals of sensory fibres) and a new mechanism (depression of synaptic transmission) for regulating respiratory reflex function.
Sekizawa
Shin-ichi
S
Department of Pharmacology and Toxicology and Department of Pediatrics, University of California Davis, Davis, CA 95616, USA.
Joad
Jesse P
JP
Bonham
Ann C
AC
D000818:Animals; D001933:Brain Stem / Q000502:physiology; D001980:Bronchi / Q000294:innervation*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000502:physiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000294:innervation*; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D017661:Receptors, Presynaptic / Q000187:drug effects*; D017552:Solitary Nucleus / Q000187:drug effects / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology
D005456:Fluorescent Dyes; D017661:Receptors, Presynaptic; D013373:Substance P; D018698:Glutamic Acid; D013779:Tetrodotoxin