title: The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model.
abstract: This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.
authors.affiliation: Matrix Biology & Tissue Repair Research Unit, Department of Dental Health & Biological Sciences, Dental School, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: R
authors.initials: R
authors.lastname: Moseley
authors.forename: R J
authors.initials: RJ
authors.lastname: Waddington
authors.forename: A J
authors.initials: AJ
authors.lastname: Sloan
authors.forename: A J
authors.initials: AJ
authors.lastname: Smith
authors.forename: R C
authors.initials: RC
authors.lastname: Hall
authors.forename: G
authors.initials: G
authors.lastname: Embery
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002327:Cariostatic Agents / Q000633:toxicity*; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004577:Electron Probe Microanalysis; D008297:Male; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D013752:Tetracycline / Q000378:metabolism
chemical_list: D002327:Cariostatic Agents; D012969:Sodium Fluoride; D013752:Tetracycline