Mycotoxin fumonisin B1 increases intestinal colonization by pathogenic Escherichia coli in pigs.
Fumonisin B(1) (FB(1)) is a mycotoxin that commonly occurs in maize. FB(1) causes a variety of toxic effects in different animal species and has been implicated as a contributing factor of esophageal cancers in humans. In the present study, we examined the effect of dietary exposure to FB(1) on intestinal colonization by pathogenic Escherichia coli associated with extraintestinal infection. Three-week-old weaned pigs were given FB(1) by gavage as a crude extract or as a purified toxin at a dose of 0.5 mg/kg of body weight daily for 6 days. On the last day of the toxin treatment, the pigs were orally inoculated with an extraintestinal pathogenic E. coli strain. All animals were euthanized 24 h later, necropsies were performed, and tissues were taken for bacterial counts and light microscopic examination. Ingestion of FB(1) had only a minimal effect on animal weight gain, did not cause any macroscopic or microscopic lesions, and did not change the plasma biochemical profile. However, colonization of the small and large intestines by an extraintestinal pathogenic E. coli strain was significantly increased. Our results show that FB(1) is a predisposing factor to infectious disease and that the pig can be used as a model for the study of the consequences of ingesting mycotoxin-contaminated food.
Oswald
Isabelle P
IP
Laboratoire de Pharmacologie-Toxicologie, Institut National de la Recherche Agronomique, Toulouse, France. ioswald@toulousse.inra.fr
Desautels
Clarisse
C
Laffitte
Joëlle
J
Fournout
Sylvie
S
Peres
Sylvie Y
SY
Odin
Marielle
M
Le Bars
Pierrette
P
Le Bars
Joseph
J
Fairbrother
John M
JM
D000284:Administration, Oral; D000818:Animals; D002274:Carcinogens, Environmental / Q000008:administration & dosage / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D037341:Fumonisins / Q000008:administration & dosage / Q000494:pharmacology*; D007150:Immunohistochemistry; D007422:Intestines / Q000382:microbiology*; D009183:Mycotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology
D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1