Rapid two-step synthesis of mitrin from heparosan: a replacement for heparin.
We have engineered a two-step enzymatic synthesis of Mitrin which is a more potent and homogeneous anticoagulant than the current animal-derived heparin. This engineered heparin may have advantages of being free from animal-derived pathogens and may also have reduced side effects such as heparin induced thrombocytopenia. This approach can also be extended to tailor heparin-based drugs with improved therapeutic characteristics to treat other disorders or infections in which heparin-like molecules play a major role.
Kuberan
Balagurunathan
B
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Beeler
David L
DL
Lawrence
Roger
R
Lech
Miroslaw
M
Rosenberg
Robert D
RD
D000925:Anticoagulants / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002240:Carbohydrate Sequence; D006025:Glycosaminoglycans / Q000138:chemical synthesis* / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
D000925:Anticoagulants; D006025:Glycosaminoglycans; D011134:Polysaccharides; C479557:mitrin; D006493:Heparin