title: Voltage-gated K(+) channel subunits on cholinergic and dopaminergic amacrine cells.
abstract: The expression of voltage-gated K+ (Kv) channel subunits on rat retinal cholinergic and dopaminergic amacrine cells was studied using double immunofluorescence labeling and confocal laser scanning microscopy. Staining for Kv3.1b was found in the cholinergic cells, being present on the membrane of somata, and on the processes, but not in the dopaminergic cells. Kv4.3-immunoreactivity was localized on the somatodendritic compartment of the dopaminergic cells, but was not found in the cholinergic cells. Differential expression between the two cell types was not found for 10 other subunits tested. These results suggest that the Kv3.1b and Kv4.3 subunits may differentially contribute to the electrophysiological properties underlying distinct functions of these two retinal interneurons.
authors.affiliation: Institute of Neurobiology, Fudan University, 220 Han-Dan Road, Shanghai 200433 PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Tian
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Zhao
authors.forename: Xiong-Li
authors.initials: XL
authors.lastname: Yang
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Xie
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D025042:Amacrine Cells / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D009479:Neuropeptides / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000378:metabolism; D051672:Shal Potassium Channels; D051670:Shaw Potassium Channels; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: C497023:Kcnd3 protein, rat; D009479:Neuropeptides; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D051672:Shal Potassium Channels; D051670:Shaw Potassium Channels; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D004298:Dopamine