[Influence of valproic acid (depakine I.V.) on human placenta metabolism--experimental model].
OBJECTIVE
The pregnancy in women with epilepsy is associated with an increased incidence of congenital malformations in offspring. Currently, anti-epileptic drugs (AEDs) are concerned to be a major etiologic factor of abnormal fetal development but the pathomechanism of teratogenicity of AEDs is complex and not well understood. The purpose of this study was to evaluate an influence of one of the AED-valproic acid (VPA) on placental metabolism (glucose consumption and lactate production).


METHODS
Term human placental cotyledons were perfused in vitro using a recycling perfusion of maternal and fetal circulations. A total 18 placentas were perfused either with 75 micrograms/ml of VPA (therapeutic dose) or with 225 micrograms/ml of VPA (toxic dose). Eight placentas were perfused with a medium without VPA and served as controls. During 2.5 h of experiment, both maternal and fetal glucose consumption and lactate production were measured every 30 minutes.


RESULTS
The introduction of different concentrations of VPA into the perfusion system did not effect placental glucose consumption and lactate production rates in both maternal and fetal compartments.


CONCLUSIONS
The teratogenic effect of valproic acid is not associated with metabolic disturbances of glucose or lactate in the placental tissue.
Semczuk-Sikora
Anna
A
Katedry i Kliniki Połoznictwa i Patologii Ciazy AM w Lublinie.
Rogowska
Wanda
W
Semczuk
Marian
M
D000014:Abnormalities, Drug-Induced / Q000209:etiology; D000927:Anticonvulsants / Q000009:adverse effects / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004827:Epilepsy / Q000188:drug therapy; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000096:biosynthesis*; D008431:Maternal-Fetal Exchange / Q000187:drug effects*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000188:drug therapy; D014635:Valproic Acid / Q000009:adverse effects / Q000494:pharmacology*
D000927:Anticonvulsants; D001786:Blood Glucose; D019344:Lactic Acid; D014635:Valproic Acid