Universal labeling chemistry for nucleic acid detection on DNA-arrays.
We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.
Bernal-Méndez
E
E
BioMérieux, Advanced Technology, Marcy l'Etoile, France. eloy.bernalmendez@eu.biomerieux.com
Tora
C
C
Sothier
I
I
Kotera
M
M
Troesch
A
A
Laayoun
A
A
D001391:Azo Compounds; D019921:Biotinylation; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction
D001391:Azo Compounds; D005456:Fluorescent Dyes; D004247:DNA