title: Effect of mild hyperglycemia +/- meta-iodo-benzylguanidine on the radiation response of R3230 Ac tumors.
abstract: The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO2) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO2 and skeletal muscle pO2 were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) +/- MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a approximately 20% inhibition of QO2 in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO2 in R3230 Ac tumors. The median tumor pO2 for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO2 and increase in tumor pO2.
authors.affiliation: Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Intae
authors.initials: I
authors.lastname: Lee
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Glickson
authors.forename: Mark W
authors.initials: MW
authors.lastname: Dewhirst
authors.forename: Dennis B
authors.initials: DB
authors.lastname: Leeper
authors.forename: Randy
authors.initials: R
authors.lastname: Burd
authors.forename: Harish
authors.initials: H
authors.lastname: Poptani
authors.forename: Lydie
authors.initials: L
authors.lastname: Nadal
authors.forename: W Gillies
authors.initials: WG
authors.lastname: McKenna
authors.forename: John E
authors.initials: JE
authors.lastname: Biaglow
mesh_terms: D019797:3-Iodobenzylguanidine / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000503:physiopathology*; D007668:Kidney / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
chemical_list: D019797:3-Iodobenzylguanidine; D005947:Glucose; D010100:Oxygen