Local application of IGF1 on dental pulp mechanically exposed; in vivo study on rabbit.
IGF1 (Insulin Growth Factor, 1) was intentionally applied onto pulp tissues, aiming to provoque a dentine regeneration process through the stimulation of the dentinoblasts' potententials. 72 cavities were hence performed on rabbit molars, intentionally exposing the dental pulp. Different concentrations of IGF1 were then applied; The histo and anatomo-pathological observations showed persistent vitality of the pulp without any sign of necrosis, even 6 weeks after the IGF1 application. Dentinoblasts layers (as an indication of the regeneration activity) were counted, according to a pre-established protocol, at days 7, 14, 22, 28 and 42. The type of the applied IGF1, was carefully selected to be "Binding Protein Resistant" (IGF-BPR), so to avoid any inhibition of the IGF1 action by the endogenous binding proteins (Hochscheid and coll). The results were conclusive in indicating the IGF1 as an efficient dental pulp capping product.
Haddad
M
M
Department of Oral Pathology, St Joseph University, Faculty of Dental Medicine, Beirut, Lebanon. mayhaddad76@hotmail.com
Lefranc
G
G
Aftimos
G
G
D000818:Animals; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000503:physiopathology*; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects; D009804:Odontoblasts / Q000187:drug effects; D011671:Pulpitis / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors
D007334:Insulin-Like Growth Factor I