Metabolic analysis of acetate accumulation during xylose consumption by Paenibacillus polymyxa.
Paenibacillus polymyxa ATCC 12321 produced more acetic acid and less butanediol from xylose than from glucose. The product yields from xylose were ethanol (0.72 mol/mol sugar), (R,R)-2,3-butanediol (0.31 mol/mol sugar), and acetate (0.38 mol/mol sugar) while those from glucose were ethanol (0.74 mol/mol sugar), (R,R)-2,3-butanediol (0.46 mol/mol sugar), and acetate (0.05 mol/mol sugar). Higher acetate kinase activity and lower acetate uptake ability were found in xylose-grown cells than in glucose-grown cells. Furthermore, phosphoketolase activity was higher in xylose-grown cells than in glucose-grown cells. In fed-batch culture on xylose, glucose feeding raised the butanediol yield to 0.56 mol/mol sugar and reduced acetate accumulation to 0.04 mol/mol sugar.
Marwoto
B
B
Department of Molecular Biotechnology, Hiroshima University, Graduate School of Advanced Sciences of Matter, 1-3-1, 739-8530 Kagamiyama, Higashi-Hiroshima, Japan.
Nakashimada
Y
Y
Kakizono
T
T
Nishio
N
N
D000084:Acetate Kinase / Q000378:metabolism*; D000085:Acetates / Q000032:analysis* / Q000378:metabolism; D000093:Acetoin / Q000032:analysis; D000446:Aldehyde-Lyases / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism; D018533:Biomass; D002072:Butylene Glycols / Q000032:analysis / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D005561:Formates / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D016936:Gram-Positive Endospore-Forming Bacteria / Q000378:metabolism*; D006859:Hydrogen / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000032:analysis; D010427:Pentose Phosphate Pathway; D019802:Succinic Acid / Q000032:analysis; D014994:Xylose / Q000378:metabolism*
D000085:Acetates; D002072:Butylene Glycols; D003470:Culture Media; D005561:Formates; C030544:formic acid; D002245:Carbon Dioxide; D019344:Lactic Acid; D000431:Ethanol; D006859:Hydrogen; D014994:Xylose; D019802:Succinic Acid; D000093:Acetoin; D000084:Acetate Kinase; D000446:Aldehyde-Lyases; C022367:phosphoketolase; D005947:Glucose