Differential gene expression in genetically matched mouse melanoma cells with different metastatic potential.
In vitro fusion of weakly metastatic Cloudman S91 melanoma cells with macrophages from DBA/2J mice (syngeneic with Cloudman S91 melanoma) produced hybrids with metastatic potentials ranging from low to high, with more than half showing enhanced metastasis over the parental melanoma [Clin. Exp. Metastasis 16 (1998) 299]. These hybrids, derived from the same parental fusion partners, represent a unique genetically matched model for analyzing differential gene expression regulating the metastatic phenotype. We have examined the differences in gene expression in metastatic fusion hybrid compared to its parental partners, non-/poorly metastatic melanoma cells and normal macrophages. An approach by selective polymerase chain reaction (PCR) amplification and display of 3' end restriction fragments of double-stranded cDNAs was used [Methods Enzymol. 303 (1999) 272]. Gene expression analyses showed an extensive set of transcripts that were up- or down-regulated in the most metastatic hybrid, H95-1, compared to the parental macrophages or melanoma cells. Sequence analyses of more than 60 of these differentially expressed cDNAs revealed significant up- or down-regulation of a number of genes known to be associated with metastasis of melanoma and other solid tumors. Some genes are found to express exclusively either in normal macrophages or in melanoma. Thirteen fragment sequences were found with no matches with GenBank search. Comparison of these gene expression patterns should be of great value in understanding the coordinate programs regulating metastasis. Further, the increased expression of gene(s) common in macrophage and fusion hybrids may be of importance in identifying the regulatory factor(s) related to macrophage-like trait, motility, a critical step of metastatic processes, in hybrids.
Chakraborty
A K
AK
Department of Dermatology, LCI 505, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. ashok.chakraborty@yale.edu
Yamaga
S
S
D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D020869:Gene Expression Profiling; D006822:Hybrid Cells / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D018076:DNA, Complementary; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific