title: Use of rpoB sequences for phylogenetic study of Mycoplasma species.
abstract: rpoB sequences encoding the beta-subunit of RNA polymerase were determined in 26 Mycoplasma species for phylogenetic study. The portion of rpoB DNA used in this study showed a high degree of variation in terms of size and sequence among species. The rpoB phylogenies inferred from amino acid and nucleotide sequences were used to divide the mycoplasmas into two groups, a 'pneumoniae group' and a 'hominis group', which was consistent with the result from 16S rDNA sequence analysis. However, phylogenetic relationships within these groups differed in the two gene trees, which were supported by the incongruence length difference (ILD) test. This indicates that multiple gene sequences must be applied to infer accurate phylogenetic relationships among the mycoplasmas. The rpoB sequence, and especially the deduced amino acid sequence, offers a good alternative marker.
authors.affiliation: Department of Laboratory Animal Sciences, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, South Korea.
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Ko
authors.forename: Myoung-Woong
authors.initials: MW
authors.lastname: Chang
authors.forename: Tae Wook
authors.initials: TW
authors.lastname: Hahn
authors.forename: Seong Karp
authors.initials: SK
authors.lastname: Hong
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
mesh_terms: D000595:Amino Acid Sequence; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014509:Ureaplasma / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit