title: Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR.
abstract: Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis.
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale, Strasbourg, France. denis.filisetti@medecine.u-strasbg.fr
authors.forename: Denis
authors.initials: D
authors.lastname: Filisetti
authors.forename: Mohamed
authors.initials: M
authors.lastname: Gorcii
authors.forename: Elodie
authors.initials: E
authors.lastname: Pernot-Marino
authors.forename: Odile
authors.initials: O
authors.lastname: Villard
authors.forename: Ermanno
authors.initials: E
authors.lastname: Candolfi
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S