The bisphosphonate pamidronate is a potent inhibitor of Ewing's sarcoma cell growth in vitro.
The MTT assay was used to measure the effects of pamidronate, clodronate and mevastatin on the cell viability of Ewing's sarcoma cell lines 6647, CADO-ES-1, ES-2, ES-3, RD-ES, SK-ES-1, STA-ET-2.1 and VH-64. Treatment of these cells with pamidronate inhibited cell viability in a time- and dose-dependent manner. After a 72-h incubation period with 50 microM pamidronate, cell numbers were reduced by up to 80%, whereas the monophosphonate analog 3-aminopropyl phosphonate had no effect at concentrations up to 2 mM. Clodronate reduced cell viability by maximally 40% at 1 mM. These data provide the first evidence for a direct growth-inhibitory effect of pamidronate on Ewing's sarcoma cells. Hence, pamidronate definitely merits a more thorough exploration into its potential use in the therapy of patients with Ewing's sarcoma.
Sonnemann
Jürgen
J
Labor für Experimentelle Orthopädie/Zellbiologie, Klinik und Poliklinik für Allgemeine Orthopädie, Universitätsklinikum Münster, Germany. Juergen.Sonnemann@uni-greifswald.de
Eckervogt
Vera
V
Truckenbrod
Borna
B
Boos
Joachim
J
Winkelmann
Winfried
W
van Valen
Frans
F
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004002:Clodronic Acid / Q000494:pharmacology; D004164:Diphosphonates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077268:Pamidronate; D012512:Sarcoma, Ewing
D000970:Antineoplastic Agents; D004164:Diphosphonates; D004002:Clodronic Acid; C012258:mevastatin; D008148:Lovastatin; D000077268:Pamidronate