Self-assembly of proteins into designed networks.
A C4-symmetric tetrameric aldolase was used to produce a quadratic network consisting of the enzyme as a rigid four-way connector and stiff streptavidin rods as spacers. Each aldolase subunit was furnished with a His6 tag for oriented binding to a planar surface and two tethered biotins for binding streptavidin in an oriented manner. The networks were improved by starting with composite units and also by binding to nickel-nitrilotriacetic acid-lipid monolayers. The mesh was adjustable in 5-nanometer increments. The production of a net with switchable mesh was initiated with the use of a calcium ion-containing beta-helix spacer that denatured on calcium ion depletion.
Ringler
Philippe
P
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, D-79104 Freiburg im Breisgau, Germany.
Schulz
Georg E
GE
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004492:Edetic Acid; D006026:Glycoside Hydrolases; D008055:Lipids / Q000737:chemistry; D046911:Macromolecular Substances; D008666:Metalloendopeptidases / Q000737:chemistry / Q000378:metabolism; D008854:Microscopy, Electron; D008958:Models, Molecular; D009154:Mutation; D009571:Nitrilotriacetic Acid; D011487:Protein Conformation; D011489:Protein Denaturation; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry*
D008055:Lipids; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D001710:Biotin; D019809:Streptavidin; D004492:Edetic Acid; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; C022361:6-phospho-beta-galactosidase; D008666:Metalloendopeptidases; C095666:serralysin; D000446:Aldehyde-Lyases; C022526:rhamnulose-1-phosphate adolase; D009571:Nitrilotriacetic Acid; D002118:Calcium