title: Origin, dispersal and genomic structure of a low-copy-number hypervariable RFLP clone in Triticum and Aegilops species.
abstract: The genome of common wheat has evolved through allopolyploidization of three ancestral diploid genomes. A previously identified restriction fragment length polymorphism (RFLP) marker, pTag546, has the unique feature of showing hypervariability among closely related common wheat cultivars. To understand the origin and the mode of dispersal of this hypervariable sequence in the wheat genome, the distribution and structure of the homologous sequences were studied using ancestral diploid species, tetraploid disomic substitution lines and synthetic hexaploid lines. Comparative Southern blot and PCR analyses suggested that pTag546 homologs in the tetraploid and hexaploid wheat were derived from the S genome of Aegilops speltoides. Some pTag546 homologs were found to have transposed to A and D genomes in polyploid wheat. Evidence of transposition and elimination in some synthetic hexaploid lines was also obtained by comparing their copy numbers with those in the parental lines. Southern blot analysis of a genomic clone using a contiguous subset of sequences as probes revealed a core region of hypervariability that coincided with the region containing pTag546. No obvious structural characteristics that could explain the hypervariability, however, were found around the pTag546 sequence, except for accumulation of small repetitive sequences at one border. It was concluded that pTag546 increased its copy number through yet unknown mechanism(s) of transposition to various chromosomal locations over the period of allopolyploid evolution and during the artificial genome manipulation in wheat.
authors.affiliation: Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture and Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Mizumoto
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takumi
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Ogihara
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nakamura
mesh_terms: D015139:Blotting, Southern; D002875:Chromosomes / Q000235:genetics; D018744:DNA, Plant; D018628:Gene Dosage; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant