title: A simple and cost-effective method for rapid genotyping of insertion/deletion polymorphisms.
abstract: We developed a simple method, based on the TaqMan technology, for fast genotyping of insertion/deletion polymorphisms of known location. The genotypes of 22 CEPH individuals, previously ascertained by conventional methods, were confirmed in the new assay without manual, time-consuming, post-PCR analysis. We propose to expand the application of TaqMan probes for population screening of insertion/deletion polymorphisms in which the exact endpoints of the insertion/deletion are known. The method can be applied to polymorphisms of any size and can be used for different applications such as diagnostics, genome variation, and species identification.
authors.affiliation: Coriell Institute for Medical Research, Camden, NJ 08103, USA. rrobledo@coriell.umdnj.edu
authors.forename: Renato
authors.initials: R
authors.lastname: Robledo
authors.forename: William
authors.initials: W
authors.lastname: Beggs
authors.forename: Patrick
authors.initials: P
authors.lastname: Bender
mesh_terms: D000483:Alleles; D001483:Base Sequence; D003365:Costs and Cost Analysis; D015342:DNA Probes / Q000235:genetics; D005838:Genotype; D016254:Mutagenesis, Insertional; D011110:Polymorphism, Genetic; D017384:Sequence Deletion
chemical_list: D015342:DNA Probes