Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR.
In order to enhance a collection of modified deoxynucleoside triphosphates useful for in vitro selection or SELEX (systematic evolution of ligands by exponential enrichment) techniques, we designed and synthesized modified analogues of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate bearing a flexible and hydrophilic 7-amino-2,5-dioxaheptyl linker at a C5 position. Both analogues were found to be substrates for thermostable DNA polymerases which belong to an evolutional family B during PCR.
Kuwahara
Masayasu
M
Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan.
Takahata
Yumi
Y
Shoji
Atsushi
A
Ozaki
Akiko N
AN
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004996:Ethidium; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D007202:Indicators and Reagents; D011741:Pyrimidine Nucleosides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004996:Ethidium