title: Molecular and physiological characterization of an invertebrate homologue of a calcitonin-related receptor.
abstract: Calcitonin is a key hormone involved in the regulation of calcium metabolism in vertebrates. Using oligonucleotide primers derived from consensus sequences of vertebrate calcitonin receptors, we have cloned and characterized the first representative of an invertebrate calcitonin receptor from the bivalve mollusc Crassostrea gigas. This receptor named Cg CT-R exhibits 39% amino acid sequence identity with both human calcitonin and calcitonin gene-related precursor receptors. Cg CT-R is expressed mainly in the gills and the mantle edge as well as at lower levels in muscles, digestive gland, heart, and labial palps. Transfer of animals from seawater to brackish water resulted in a significant decrease of Cg CT-R transcript levels in the gills, thus suggesting a role for ionic balance in molluscs.
authors.affiliation: Laboratoire de Biologie et Biotechnologies Marines, IBFA, UMR IFREMER-Université de Caen Physiologie et Ecophysiologie des Mollusques Marins, Esplanade de la Paix, 14032 Caen Cedex, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Dubos
authors.forename: Fabien
authors.initials: F
authors.lastname: Badariotti
authors.forename: Franck
authors.initials: F
authors.lastname: Rodet
authors.forename: Christophe
authors.initials: C
authors.lastname: Lelong
authors.forename: Pascal
authors.initials: P
authors.lastname: Favrel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism; D007477:Ions; D008969:Molecular Sequence Data; D008974:Mollusca; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007477:Ions; D012333:RNA, Messenger; D018015:Receptors, Calcitonin Gene-Related Peptide; D043562:Receptors, G-Protein-Coupled