title: Precursor-Directed polyketide biosynthesis in Escherichia coli.
abstract: Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.
authors.affiliation: Department of Chemistry, Box H, Brown University, Providence, RI 02912-9108, USA.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Blaine A
authors.initials: BA
authors.lastname: Pfeifer
authors.forename: Chaitan
authors.initials: C
authors.lastname: Khosla
authors.forename: David E
authors.initials: DE
authors.lastname: Cane
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011498:Protein Precursors / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes; D011498:Protein Precursors