Assessment of adhesion formation to intra-abdominal polypropylene mesh and polytetrafluoroethylene mesh.
BACKGROUND
The development of intra-abdominal adhesions, bowel obstruction, and enterocutaneous fistulas are potentially severe complications related to the intraperitoneal placement of prosthetic biomaterials. The purpose of this study was to determine the natural history of adhesion formation to polypropylene mesh and two types of polytetrafluoroethylene (ePTFE) mesh when placed intraperitoneally in a rabbit model that simulates laparoscopic ventral hernia repair.


METHODS
Thirty New Zealand white rabbits were used for this study. A 10-cm midline incision was performed for intra-abdominal access and a 2 cm x 2 cm piece of mesh (n = 60) was sewn to an intact peritoneum on each side of the midline. Two types of ePTFE mesh (Dual Mesh and modified Dual Mesh, W.L. Gore & Assoc., Flagstaff, AZ) and polypropylene mesh were compared. The rate of adhesion formation was evaluated by direct visualization using microlaparoscopy (2-mm endoscope/trocar) at 7 days, 3 weeks, 9 weeks, and 16 weeks after mesh implantation. Adhesions to the prosthetic mesh were scored for extent (%) using the Modified Diamond Scale (0 = 0%, 1 <or= 25%, 2 = 25-50%, 3 > 50%). At necropsy the mesh was excised en bloc with the anterior abdominal wall for histological evaluation of mesothelial layer growth.


RESULTS
The mean adhesion score for the polypropylene mesh was significantly greater (P < 0.05) than Dual Mesh at 9 weeks and 16 weeks and modified Dual Mesh at 7 days, 9 weeks, and 16 weeks. Fifty-five percent (n = 11) of the polypropylene mesh had adhesions to small intestine or omentum at necropsy compared to 30% (n = 6) of the Dual Mesh and 20% (n = 4) of the modified Dual Mesh. There was a significantly greater percentage (P < 0.003) of ePTFE mesh mesothelialized at explant (modified Dual Mesh 44.2%; Dual Mesh 55.8%) compared to the polypropylene mesh (12.9%).


CONCLUSIONS
Serial microlaparoscopic evaluation of intraperitoneally implanted polypropylene mesh and ePTFE mesh in a rabbit model revealed a progression of adhesions to polypropylene mesh over a 16 week period. The pore size of mesh is critical in the development and maintenance of abdominal adhesions and tissue ingrowth. The macroporous polypropylene mesh promoted adhesion formation, while the microporous nature of the visceral side of the ePTFE served as a barrier to adhesions.
Matthews
Brent D
BD
Department of General Surgery, and The James B Cannon Research Center, Carolinas Medical Center, Charlotte, North Carolina 28203, USA.
Pratt
Broc L
BL
Pollinger
Harrison S
HS
Backus
Charles L
CL
Kercher
Kent W
KW
Sing
R F
RF
Heniford
B Todd
BT
D000005:Abdomen; D000818:Animals; D001672:Biocompatible Materials; D010535:Laparoscopy / Q000009:adverse effects / Q000379:methods; D008422:Materials Testing; D009757:Nylons; D011095:Polyethylenes; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D011817:Rabbits; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000473:pathology* / Q000517:prevention & control
D001672:Biocompatible Materials; D009757:Nylons; D011095:Polyethylenes; D011138:Polytetrafluoroethylene