title: Isolation and identification of an antioxidant enzyme catalase stimulatory compound from Garnoderma lucidum.
abstract: Antioxidant enzymes are scavenger reactive-oxygen intermediates and are involved in many cellular defense systems. We previously reported that a crude extract of Garnoderma lucidum, a medicinally potent mushroom, profoundly increased the catalase gene expression and enzyme activities in mouse livers (Park et al., J. Biochem. Mol. Biol. 34. 144-149, 2001). In this study, we elucidated the detailed mechanism whereby G. lucidum stimulates the catalase activity and expression. The major active fraction was isolated from G. lucidum and methyl linoleate was considered the most major component of the fraction. In order to determine whether methyl linoleate increases mRNA and protein synthesis of catalase, Northern and Western blot analyses were performed in vivo with methyl linoleate-treated mouse liver homogenate after feeding methyl linoleate to the mice. Northern and Western blot analyses of the crude liver homogenates in the mice that were administered methyl linoleate revealed that the expression catalase was significantly increased when compared to the untreated controls. In addition, the catalase protein levels and enzymatic activities increased in the mouse liver homogenates. These results suggest that methyl linoleate that is produced by G. lucidum stimulates the catalase expression at the transcription level.
authors.affiliation: Division of Life Sciences, Hallym University, Chunchon 200-702, Korea.
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Byung Ryong
authors.initials: BR
authors.lastname: Lee
authors.forename: Chang Sik
authors.initials: CS
authors.lastname: Yoon
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Choi
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D020536:Enzyme Activators / Q000494:pharmacology*; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D008041:Linoleic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D020536:Enzyme Activators; D008041:Linoleic Acids; C005575:methyl linoleate; D002374:Catalase