title: Production of ribosome components in effector CD4+ T cells is accelerated by TCR stimulation and coordinated by ERK-MAPK.
abstract: Effector CD4+ T cells rapidly activate high-level cytokine expression following TCR stimulation. Consistent with accelerated protein production in these cells, global mRNA profiles revealed that, after cytokines, the most impressive cluster of activated genes encode rRNA-maturation factors. Activation of these genes was ERK-MAPK dependent, accompanied by increased rRNA transcription and faster maturation kinetics, and much greater in effector CD4+ T cells than in naive cells. Ribosomal protein subunit (RPS) synthesis was also ERK-MAPK dependent and increased to match rRNA production, but without evident increase in RPS mRNA. Instead, stimulation promoted polysome loading of RPS mRNA via cis-acting, 5'-terminal oligopyrimidines. These results demonstrate how, in response to extracellular signals, effector CD4+ T cells coordinately increase multiple ribosomal components to accommodate burgeoning cytokine production.
authors.affiliation: Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Asmal
authors.forename: John
authors.initials: J
authors.lastname: Colgan
authors.forename: Felix
authors.initials: F
authors.lastname: Naef
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Youngnam
authors.initials: Y
authors.lastname: Lee
authors.forename: Marcelo
authors.initials: M
authors.lastname: Magnasco
authors.forename: Jeremy
authors.initials: J
authors.lastname: Luban
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D016337:RNA, Catalytic / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D011506:Proteins; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D020928:Mitogen-Activated Protein Kinases