title: Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase.
abstract: The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase-p37-that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation.
authors.affiliation: Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Almeida
authors.forename: Odília
authors.initials: O
authors.lastname: Queirós
authors.forename: Alan
authors.initials: A
authors.lastname: Wheals
authors.forename: José
authors.initials: J
authors.lastname: Teixeira
authors.forename: Pedro
authors.initials: P
authors.lastname: Moradas-Ferreira
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002473:Cell Wall / Q000235:genetics* / Q000502:physiology; D005427:Flocculation; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D014170:Transformation, Genetic / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D012331:RNA, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases