Induction of protective immunity against malaria by priming-boosting immunization with recombinant cold-adapted influenza and modified vaccinia Ankara viruses expressing a CD8+-T-cell epitope derived from the circumsporozoite protein of Plasmodium yoelii.
We immunized mice with an attenuated (cold-adapted) influenza virus followed by an attenuated vaccinia virus (modified vaccinia virus Ankara), both expressing a CD8(+)-T-cell epitope derived from malaria sporozoites. This vaccination regimen elicited high levels of protection against malaria. This is the first time that the vaccine efficacy of a recombinant cold-adapted influenza virus vector expressing a foreign antigen has been evaluated.
González-Aseguinolaza
Gloria
G
Department of Medical & Molecular Parasitology, NYU School of Medicine. Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Nakaya
Yurie
Y
Molano
Alberto
A
Dy
Edward
E
Esteban
Mariano
M
Rodríguez
Dolores
D
Rodríguez
Juan Ramón
JR
Palese
Peter
P
García-Sastre
Adolfo
A
Nussenzweig
Ruth S
RS
D000222:Adaptation, Physiological; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003080:Cold Temperature; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D008288:Malaria / Q000276:immunology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009975:Orthomyxoviridae / Q000235:genetics / Q000276:immunology*; D010967:Plasmodium yoelii / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*
D000913:Antibodies, Protozoan; D018984:Epitopes, T-Lymphocyte; D017780:Malaria Vaccines; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan