title: Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: preliminary results.
abstract: Using a spleen autotransplantation model, we conducted hematological, hemorheological, immunological, and morphological studies in mice 6 weeks after splenectomy. Sixty male and female A/J inbred mice were equally divided into 3 groups: 1) SE group, splenectomy was performed; 2) AU group, spleen chips were autotransplanted into the omentum without vascular anastomosis following splenectomy; and 3) C group (controls), no intervention in these mice. At postoperative week 6, the following studies were performed: 1) measurement of hematological parameters; 2) hemorheological studies, including relative cell transit time (RCTT) and fibrinogen levels; and 3) activity of peripheral phagocytes, measured by zymozan-induced chemiluminescence, which was calculated in stimulation index values (SI). In addition, histological investigations of autotransplants were conducted. Erythrocyte mean cell volume and platelet counts, RCTT, fibrinogen levels, and activity of phagocytes were significantly higher in the SE group, compared to those in the C group. In the AU group, these parameters were similar to those in the C group. Morphologically, the transplanted spleen showed normal histology. These data indicate that the transplanted spleens restored their function. We conclude that spleen autotransplantation reserves the normal morphology of spleen and restores most of the spleen's hematological, hemorheological, and immunological functions. Both SI index and erythrocyte deformability can be an informative detection of decreasing splenic function. These data suggest that spleen autotransplantation may provide a useful tool to prevent complications following splenectomy in a clinical setting.
authors.affiliation: Department of Operative Techniques and Surgical Research, Medical and Health Science Center, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. imiko@jaguar.dote.hu
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
authors.forename: Endre
authors.initials: E
authors.lastname: Brath
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.forename: Ferenc F
authors.initials: FF
authors.lastname: Toth
authors.forename: Sandor
authors.initials: S
authors.lastname: Sipka
authors.forename: Judit
authors.initials: J
authors.lastname: Kovacs
authors.forename: Sandor
authors.initials: S
authors.lastname: Sipka
authors.forename: Jozsef
authors.initials: J
authors.lastname: Fachet
authors.forename: Andrea
authors.initials: A
authors.lastname: Furka
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
authors.forename: Robert
authors.initials: R
authors.lastname: Zhong
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D009852:Omentum / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology; D013154:Spleen / Q000098:blood supply / Q000637:transplantation*; D013156:Splenectomy; D014182:Transplantation, Autologous / Q000379:methods / Q000473:pathology / Q000502:physiology*