[Effects of nitric oxide synthase inhibitor on dentate gyrus neurogenesis after diffuse brain injury in adult rats].
OBJECTIVE
To investigate the effects of selective nitric oxide synthase (NOS) inhibitors on dentate gyrus (DG) neurogenesis after diffuse brain injury (DBI) in adult rats.


METHODS
DBI models were established in adult male SD rats, followed by systemic bromodeoxyuridine (BrdU) labeling of the dividing cells and immunohistochemical assay of the proliferation rates of neural precursor cells in the DG for comparison between NOS inhibitor (7-nitroindazole and Aminoguanidine) groups and the corresponding control groups at various time points after DBI.


RESULTS
Intraperitoneal administration of 7-nitroindazole significantly reduced the number of BrdU-labeled cells in the DG of adult rats 2, 4 and 6 d after DBI (P<0.05). Aminoguanidine also significantly inhibited the proliferation of neural precursor cells in the DG induced by DBI at various time points (P<0.01).


CONCLUSIONS
The activation of NOS after DBI may be an important regulatory factor for DG neurogenesis in adult rats, and NO generated by nNOS is probably involved mainly in the early stage of enhanced neurogenesis after DBI, while the NO from iNOS might participated primarily in the later stages.
Cheng
Yu-hua
YH
Department of Neurosurgery, Jiangxi Headquater Hospital of Chinese People's Armed Police Forces, Nanchang 330001, China.
Wang
Wei-dong
WD
Sun
Li-sha
LS
Cheng
Zu-jue
ZJ
Xu
Jiang-ping
JP
D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D018891:Dentate Gyrus / Q000187:drug effects* / Q000503:physiopathology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology
D004791:Enzyme Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; D019001:Nitric Oxide Synthase