Neuronal and muscular alterations caused by two wheat endosperm proteins, puroindoline-a and alpha1-purothionin, are due to ion pore formation.
Using the patch-clamp technique it was found that the toxicity of the two wheat endosperm proteins puroindoline-a and alpha1-purothionin probably results from the dissipation of ion concentration gradients essential for the maintenance of cellular homeostasis.
Llanos
Paola
P
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia No. 1027, Casilla 70005, Santiago 6530499, Chile.
Henriquez
Mauricio
M
Minic
Jasmina
J
Elmorjani
Khalil
K
Marion
Didier
D
Riquelme
Gloria
G
Molgó
Jordi
J
Benoit
Evelyne
E
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006706:Homeostasis; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009469:Neuromuscular Junction; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018272:Porins / Q000378:metabolism*; D016062:Porosity
D023181:Antimicrobial Cationic Peptides; D008081:Liposomes; D010940:Plant Proteins; D018272:Porins; C082942:puroindoline protein, Triticum aestivum; C063948:alpha1-purothionin protein, wheat