Interleukin-10 influences the expression of MRP8 and MRP14 in human dendritic cells.
BACKGROUND
We investigated the differential gene expression of human dendritic cells (DC) under the influence of the cytokine interleukin-10 (IL-10).


METHODS
The gene expression profiles of IL-10-treated DC and untreated DC (untreated control) were compared using cDNA expression array.


RESULTS
In this array, several differentially expressed transcripts could be identified. Two of them, the transcripts for the myeloid-related proteins (MRPs) MRP8 and MRP14, were examined more closely. The transcripts encoding for these proteins were expressed in mature untreated DC, and significantly induced in IL-10-treated DC.


CONCLUSIONS
These data demonstrate that the genes for MRP8 and MRP14 are expressed in various DC subpopulations in different intensity, depending on their maturational status and are increased after the treatment with IL-10. Expression of the genes for MRP8 and MRP14 in DC has not been reported before. These results point to different, still unknown functions of these two proteins in DC: to a role in DC maturation, and possibly to an influence on the creation of tolerogenic T cells under the influence of IL-10.
Kumar
Anita
A
Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.
Steinkasserer
Alexander
A
Berchtold
Susanne
S
D015152:Blotting, Northern; D015139:Blotting, Southern; D040501:Calgranulin A / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D040502:Calgranulin B / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects
D040501:Calgranulin A; D040502:Calgranulin B; D016753:Interleukin-10; D012313:RNA