[Effect of a low concentration of hydrogen peroxide on metabolism of blood cells].
Hydrogen peroxide is known to posses a wide range of physiological effects towards functional activity of cells. We have investigated the influence of H2O2 on the activity of glycolys in the native blood cells. Adding of hydrogen peroxide up to final concentration 50 microM led to decrease of activity some glycolytic enzymes. H2O2 inhibited consumption of glucose by blood cells. Despite the fact that H2O2 is well known as prooxidant, we observed decrease of the level of thiobarbituric acid reactive species in the blood cells after their incubation in the presence of hydrogen peroxide for 2 hours. Based on these data we supposed that effects of hydrogene peroxide occur via signal transduction pathways.
Samokhvalov
V A
VA
Saratov State University, Saratov, Astrakhanskaya str 83, 410026 Russia. samokhvalov@mail.ru
Smetanina
M D
MD
Museĭkina
N Iu
NIu
Mel'nikov
G V
GV
Fedotova
O V
OV
Ignatov
V V
VV
D000818:Animals; D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide