Generation of reactive oxygen species from Hinokitiol under near-UV irradiation.
Near-UV irradiation caused the decomposition of hinokitiol in an aqueous solution. During the photochemical reaction, the distinct electron spin resonance signal characteristic of the adduct of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) with the hydroxyl radical was accompanied by small signals corresponding to the adduct of DMPO with the superoxide anion radical. More than 95% of Escherichia coli cells were killed by the incubation with hinokitiol under near-UV irradiation by BLB fluorescent lamps. These results indicated the generation of reactive oxygen species during photochemical reaction of hinokitiol under near-UV irradiation.
Shibata
Hitoshi
H
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan. shibata@life.shimane-u.ac.jp
Nagamine
Takayuki
T
Wang
Yong
Y
Ishikawa
Takahiro
T
Sawa
Yoshihiro
Y
D015169:Colony Count, Microbial; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000166:cytology / Q000528:radiation effects; D017665:Hydroxyl Radical / Q000138:chemical synthesis; D039821:Monoterpenes / Q000737:chemistry* / Q000528:radiation effects; D010782:Photolysis; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000138:chemical synthesis; D014334:Tropolone / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays
D039821:Monoterpenes; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; D014334:Tropolone; C009479:beta-thujaplicin