Serotonin reduces potassium current in rutabaga and wild-type Drosophila neurons.
The Drosophila learning mutant rutabaga is defective in short-term memory and has a reduced intracellular cyclic adenosine monophosphate (cAMP) concentration. The delayed-rectifier potassium current (IKDR) was measured from cultured (2 days) wild-type and rutabaga neurons. IKDR was smaller in rutabaga neurons (382 +/- 41 pA) than in wild-type neurons (542 +/- 33 pA). IKDR was measured from neurons before and after addition of serotonin to the external solution. IKDR was reduced by serotonin in wild type (decreasing 37 +/- 7%) and rutabaga (decreasing 33 +/- 6%) neurons (single-cell studies). In the presence of serotonin, IKDR was smaller in rutabaga (218 +/- 24 pA) than in wild-type (426 +/- 35 pA) neurons (population studies). These results indicate that serotonin has affected IKDR so that the inherent difference between the two genotypes was preserved.
Alshuaib
Waleed B
WB
Department of Physiology, Faculty of Medicine, Kuwait University, Safat, Kuwait. waleed@hsc.kuniv.edu.kw
Mathew
Mini V
MV
Hasan
Mohamed Y
MY
Fahim
Mohamed A
MA
D000818:Animals; D030801:Animals, Genetically Modified; D029688:Brassica napus; D048429:Cell Size; D002478:Cells, Cultured; D004330:Drosophila; D008564:Membrane Potentials / Q000187:drug effects; D008569:Memory Disorders / Q000235:genetics / Q000503:physiopathology*; D009154:Mutation; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*
D015221:Potassium Channels; D012701:Serotonin; D011188:Potassium