title: Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing.
abstract: A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up.
authors.affiliation: Amersham Biosciences, Piscataway, New Jersey 08855-1327, USA.
authors.forename: Satyam
authors.initials: S
authors.lastname: Nampalli
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Finn
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Haiguang
authors.initials: H
authors.lastname: Xiao
authors.forename: John R
authors.initials: JR
authors.lastname: Nelson
authors.forename: Gregory
authors.initials: G
authors.lastname: Grossmann
authors.forename: Parke K
authors.initials: PK
authors.lastname: Flick
authors.forename: Carl W
authors.initials: CW
authors.lastname: Fuller
authors.forename: Shiv
authors.initials: S
authors.lastname: Kumar
mesh_terms: D001483:Base Sequence; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis*; D015224:Dideoxynucleosides / Q000138:chemical synthesis*; D004735:Energy Transfer; D007202:Indicators and Reagents
chemical_list: D004396:Coloring Agents; D003854:Deoxyribonucleotides; D015224:Dideoxynucleosides; D007202:Indicators and Reagents; D004247:DNA