Liquid chromatography determination of 10-hydroxycamptothecin in human serum by a column-switching system containing a pre-column with restricted access media and its application to a clinical pharmacokinetic study.
A simple, rapid, sensitive column-switching HPLC method is described for the analysis of the 10-hydroxycamptothecin (HCPT) in human serum. A pre-column containing restricted access media (RAM) is used for the sample clean-up and trace enrichment and is combined with a C18 column for the final separation. The analytical time is 8 min. The HCPT is monitored with fluorescence detector, excitation and emission wavelengths being 385 and 539 nm, respectively. There is a linear response range of 1-1000 ng/ml with correlation coefficient of 0.998 while the limit of quantification is 0.1 ng/ml. The intra-day and inter-day variations are less than 5%. This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model are calculated.
Ma
Jun
J
Department of Pharmacy, General Hospital of Lanzhou Command of PLA, Lanzhou 730050, China. mb1642@public.lz.gs.cn
Jia
Zheng-Ping
ZP
Zhang
Qiang
Q
Fan
Jun-Jie
JJ
Jiang
Ning-Xi
NX
Wang
Rong
R
Xie
Hua
H
Wang
Juan
J
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D012016:Reference Values; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000972:Antineoplastic Agents, Phytogenic; C028098:10-hydroxycamptothecin; D002166:Camptothecin