Morphological changes on small-bowel fetal allografts in mice.
The aim of this study was to evaluate the early morphological development and acute rejection process in fetal intestine allografts. Grafts from C57BL/6 fetal intestines were implanted in an avascular form in BALB/C recipients. A syngeneic group of animals was used to compare the evolution. The allogeneic recipients were distributed in 6 groups, according to the day of sacrifice (3rd, 4th, 5th, 6th, 7th, and 10th postoperational day (POD)) and the control group on the 2nd, 5th, and 7th POD. These grafts were stained with hematoxylin and eosin for histological evaluation, in agreement with the classification of Auber et al. (Chirurgie 123:122-130, 1998). Data showed a progressive development of the graft until POD 5. On POD 3 and 4, a top grade of development and an initial rejection were observed. From POD 5-7 and on POD 10, the acute rejection reaction was more important than the development process. The higher level of rejection was observed on POD 10, and it was similar to the 7th POD. Our results showed good graft development until POD 5. After that, the acute rejection response impeded analysis of the development process.
Resende
Vanessa Contato Lopes
VC
Disciplina de Técnica Operatória e Cirurgia Experimental, Departamento de Cirurgia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Montero
Edna Frasson de Souza
EF
Leão
Jovelino Quintino De Souza
JQ
Manna
Mônica Cecília Bochetti
MC
Koike
Márcia Kiyomi
MK
Pedrosa
Marcelo Eduardo
ME
Simões
Manuel De Jesus
Mde J
D000818:Animals; D005260:Female; D016332:Fetal Tissue Transplantation / Q000276:immunology* / Q000379:methods; D006084:Graft Rejection / Q000276:immunology*; D007421:Intestine, Small / Q000196:embryology / Q000637:transplantation*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D014184:Transplantation, Homologous / Q000276:immunology* / Q000379:methods