Substrate 2'-hydroxyl groups required for ribozyme-catalyzed polymerization.
A polymerase ribozyme has been generated that uses nucleoside triphosphates to elongate an RNA primer by the successive addition of nucleotides complementary to an RNA template. Its polymerization is accurate, with an average error rate less than 3%, and it is general in terms of the sequence and the length of the primer and template RNAs. To begin to understand how the substrate contacts contribute to this accurate and general activity, we investigated which primer and template 2'-hydroxyl groups are involved in substrate recognition. We identified eight positions where 2'-deoxy substitutions can influence polymerization kinetics. All eight are within five nucleotides of the primer 3' terminus. Some, but not all, of the 2'-deoxy effects appear to be sequence dependent. These results begin to build a picture of how the polymerase ribozyme recognizes its substrates.
Müller
Ulrich F
UF
Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA.
Bartel
David P
DP
D002384:Catalysis; D017665:Hydroxyl Radical; D007700:Kinetics; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000096:biosynthesis*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013698:Templates, Genetic
C034477:RNA primers; D016337:RNA, Catalytic; D012330:RNA, Double-Stranded; D017665:Hydroxyl Radical; D012313:RNA; D012316:RNA Nucleotidyltransferases