title: The effect of commercial steam pasteurization on the levels of Enterobacteriaceae and Escherichia coli on naturally contaminated beef carcasses.
abstract: The aim of the study was to assess the reduction achieved by steam pasteurization of beef carcasses of Escherichia coli, Enterobacteriaceae and total aerobic mesophilic plate counts (APCs). In total, 30 carcass halves were exposed to steam pasteurization (90 degrees C, 10 s exposure time) and the 30 corresponding carcass halves remained as untreated controls. The neck, midline and rump were sampled on each carcass half. Significant reductions in E. coli incidence (P < 0.05) and counts, 0.5 log10 CFU 1000 cm(-2) (P < 0.05), were observed on rump sites only. Significant reductions (>0.8 log10 CFU 1000 cm(-2)) of Enterobacteriaceae were observed at all carcass sites sampled (P < 0.05). Enterobacteriaceae reductions (>2 log10 CFU 1000 cm(-2)) were highly significant at the more contaminated sites (P < 0.001). Reductions in total APCs were inconsistent. Steam pasteurization significantly reduced the level of E. coli and Enterobacteriaceae at more contaminated sites, but did not result in complete decontamination. Therefore, steam pasteurization should be classed as an aid to hygienic beef processing, but not as a critical control point.
authors.affiliation: Department of Agriculture and Food, Central Research Veterinary Laboratory, Abbotstown, Castleknock, Dublin 15, Ireland. donal.minihan@agriculture.gov.ie
authors.forename: D
authors.initials: D
authors.lastname: Minihan
authors.forename: P
authors.initials: P
authors.lastname: Whyte
authors.forename: M
authors.initials: M
authors.lastname: O'Mahony
authors.forename: J D
authors.initials: JD
authors.lastname: Collins
mesh_terms: D000003:Abattoirs / Q000592:standards*; D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005506:Food Contamination / Q000517:prevention & control*; D007494:Ireland / Q000453:epidemiology; D008461:Meat Products / Q000382:microbiology*; D013227:Steam; D013242:Sterilization
chemical_list: D013227:Steam