title: Establishment and characterization of unique cell lines derived from pyothorax-associated lymphoma which develops in long-standing pyothorax and is strongly associated with Epstein-Barr virus infection.
abstract: Malignant lymphoma frequently develops in the pleural cavity of patients with over 20 years' history of pyothorax. The term pyothorax-associated lymphoma (PAL) has been proposed for this type of tumor. We established four novel lymphoma cell lines (OPL-3, -4, -5, and -7) from four patients with PAL. Characteristics of the four cell lines are as follows: B-cell nature with defective expression of B-cell and T-cell surface antigens, monoclonal pattern of Epstein-Barr virus (EBV) infection in lymphoma cells (thus indicating an etiological role of EBV for lymphomagenesis), complicated chromosomal abnormalities with numerous structural and numerical abnormalities, and occasional but distinct genome instability. These abnormalities in cell character might be caused by the specific circumstances of PAL lymphomagenesis, i.e., chronic inflammation. Thus, PAL cell lines could be useful in analysis of molecular mechanisms leading to malignancy in chronic inflammation.
authors.affiliation: Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takakuwa
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Luo
authors.forename: Maria Francisca
authors.initials: MF
authors.lastname: Ham
authors.forename: Masao
authors.initials: M
authors.lastname: Mizuki
authors.forename: Keiji
authors.initials: K
authors.lastname: Iuchi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015139:Blotting, Southern; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D016724:Empyema, Pleural / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D005260:Female; D005434:Flow Cytometry; D015321:Gene Rearrangement / Q000235:genetics; D019672:Genes, T-Cell Receptor / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000150:complications / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype
chemical_list: D007143:Immunoglobulin Heavy Chains