title: [Study on anti-tumor activity of extracts from cultured cells of Taxus chinensis].
abstract: OBJECTIVE
  To analyze the cytotoxicity and inhibitory effect of extracts from cultured cells(from stems) of Taxus chinensis on cancer cell line SMMC-7721.
  
  
  METHODS
  MTT assay for cell viability and flow cytometry for cell cycle analysis.
  
  
  RESULTS
  MTT results showed that the extact by dichloromethane was more effective than that by methanol on SMMC-7721, IC50 were 0.0834 mg DCW.ml-1 and 0.8002 mg DCW.ml-1 respectively. SMMC-7721 cells in G2-M stage increased with higher concentration of dichloromethane extracts from Taxus chinensis cells and longer incubation.
  
  
  CONCLUSIONS
  Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC-7721 and could induce apoptosis of cancer cells.
authors.affiliation: School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074.
authors.forename: Cuitao
authors.initials: C
authors.lastname: Lu
authors.forename: Xingguo
authors.initials: X
authors.lastname: Mei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002453:Cell Cycle; D002478:Cells, Cultured / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020946:Taxus / Q000737:chemistry / Q000166:cytology*; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal