Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR.
Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis.
Filisetti
Denis
D
Institut de Parasitologie et de Pathologie Tropicale, Strasbourg, France. denis.filisetti@medecine.u-strasbg.fr
Gorcii
Mohamed
M
Pernot-Marino
Elodie
E
Villard
Odile
O
Candolfi
Ermanno
E
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000469:parasitology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S