[Effect of apoptosis induced by different vitamin E homologous analogues in human hepatoma cells(HepG2)].
To observe the effect of apoptosis induced by alpha-tocopherol(alpha-T), gamma-tocopherol(gamma-T), delta-tocopherol(delta-T), and vitamin E succinate (VES) in human hepatoma cells (HepG2), apoptosis was detected by flow cytometry (FCM) and DNA ladder with different VE homologues analogues (alpha-T, gamma-T, delta-T and VES) and different concentrations (12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L) at 48 h. The growth of HepG2 cells was inhibited by delta-T and VES at 12.5-200 mg/L in comparison with the negative control group, while (-T showed weak effect of inhibition and alpha-T did not show any inhibition effect. However, as an exception, alpha-tocopherol, delta-tocopherol and VES were effective in induction of apoptosis in HepG2 cells at concentrations of 12.5-200 mg/L. gamma-tocopherol showed effect only at 200 mg/L. Conclusions delta-tocopherol and VES lowered HepG2 cells growth and viability, and increased apoptotic propensity significantly. A dose-dependent of antiproliferation and induction apoptosis was found in HepG2 cells line. The order of efficiency of four vitamin E analogues was delta-tocopherol > VES > gamma-tocopherol > alpha-tocopherol. The difference in nature and magnitude of the anticancer effects did not correlate with their reported antioxidant activity and this might be due to minor differences in their structure important to their biological activities. The results from this study suggested that delta-tocopherol and VES could be the promising anti-hepatoma agents.
Min
Junxia
J
Second Military Medical University, Shanghai 200433, China.
Guo
Junsheng
J
Zhao
Faji
F
Cai
Donglian
D
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D024505:Tocopherols / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology; D024504:gamma-Tocopherol / Q000494:pharmacology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D014810:Vitamin E; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol; C479072:delta-tocopherol; D024505:Tocopherols