Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis strain RJ-11.
Lactic acid bacteria exhibiting activity against the gram-positive bacterium Bacillus subtilis were isolated from rice bran. One of the isolates, identified as Enterococcus faecalis RJ-11, exhibited a wide spectrum of growth inhibition with various gram-positive bacteria. A bacteriocin purified from culture fluid, designated enterocin RJ-11, was heat stable and was not sensitive to acid and alkaline conditions, but it was sensitive to several proteolytic enzymes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that enterocin RJ-11 had a molecular weight of 5,000 in its monomeric form. The amino acid sequence determined for purified enterocin RJ-11 exhibited high levels of similarity to the sequences of enterocins produced by Enterococcus faecium.
Yamamoto
Yukio
Y
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan.
Togawa
Yoshikazu
Y
Shimosaka
Makoto
M
Okazaki
Mitsuo
M
D000595:Amino Acid Sequence; D001419:Bacteria / Q000187:drug effects*; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D003470:Culture Media; D010450:Endopeptidases / Q000378:metabolism; D013293:Enterococcus faecalis / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology
D001430:Bacteriocins; D003470:Culture Media; D010450:Endopeptidases