Isolation and characterization of phenol-catabolizing bacteria from a coking plant.
New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a Vmax of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.
El-Sayed
Wael S
WS
Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Ibrahim
Mohamed K
MK
Abu-Shady
Mohamed
M
El-Beih
Fawkia
F
Ohmura
Naoya
N
Saiki
Hiroshi
H
Ando
Akikazu
A
D000332:Aerobiosis; D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D003077:Coke / Q000382:microbiology*; D007700:Kinetics; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000447:Aldehydes; D002396:Catechols; D003077:Coke; D010636:Phenols