title: Plasmalogens protect unsaturated lipids against UV-induced oxidation in monolayer.
abstract: Oxidative stress results from the attack by free radicals of several cellular targets (proteins, DNA and lipids). The cell equilibrium is a direct consequence of the pro-/antioxidant balance. In order to understand the physiological processes involved in oxidative stress, we followed oxidation of unsaturated lipids using a biomimetic system: Langmuir monolayers. The oxidation mode chosen was UV-irradiation and the lipid model was a polyunsaturated phospholipid: 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC). The monomolecular film technique was used to measure membrane rheology before and after UV-irradiation. We showed that the UV-irradiation of a DLPC monomolecular film led to a molecular area and surface elasticity modulus decrease that attests to the apparition of new molecular species at the air-water interface. The antioxidant effect of a synthetic plasmalogen (1-O-(1'-(Z)-hexadecenyl)-2-O-oleoyl-sn-glycero-3-phosphocholine or P(PLM)OPE) was tested on the oxidation of DLPC. Indeed, for about 25% mol P(PLM)OPE in mixed DLPC/P(PLM)OPE monolayers, a complete inhibition of the molecular area and the surface elasticity modulus decreases was observed in our experimental conditions. Lower P(PLM)OPE quantities delayed but did not prevent the DLPC oxidation in mixed monolayers.
authors.affiliation: Laboratoire de Physico-Chimie Biologique, UMR-CNRS 5013, Bât. E. Chevreul, Université Claude Bernard Lyon I, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France. morandat@univ-lyon1.fr
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morandat
authors.forename: Muriel
authors.initials: M
authors.lastname: Bortolato
authors.forename: Geneviève
authors.initials: G
authors.lastname: Anker
authors.forename: Alain
authors.initials: A
authors.lastname: Doutheau
authors.forename: Michel
authors.initials: M
authors.lastname: Lagarde
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Chauvet
authors.forename: Bernard
authors.initials: B
authors.lastname: Roux
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D050356:Lipid Metabolism; D008055:Lipids / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000378:metabolism; D010955:Plasmalogens / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D008055:Lipids; D010713:Phosphatidylcholines; D010955:Plasmalogens; D011837:Radiation-Protective Agents; C002448:1,2-linoleoylphosphatidylcholine