Antisense knockdown of PKC-alpha using LNA-oligos.
Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase C-alpha (PKC-alpha) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos.
Hansen
Jens Bo
JB
Cureon A/S, Copenhagen, Denmark. bh@cureon.com
Westergaard
Majken
M
Thrue
Charlotte Albaek
CA
Giwercman
Birgit
B
Oerum
Henrik
H
D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011493:Protein Kinase C / Q000235:genetics*; D051571:Protein Kinase C-alpha; D013873:Thionucleotides; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; C477371:locked nucleic acid; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha