title: [Cloning and expression of hepatitis C core protein gene].
abstract: OBJECTIVE
  To express hepatitis C virus (HCV) core protein gene fragment in E. coli.
  
  
  METHODS
  A fragment of HCV core gene sequence 357 bp in length was amplified by PCR, digested with EcoRI+Hind III and inserted to the plasmid vector pET-32a to construct recombinant HCVc/pET-32a plasmid, which was transformed into E.coli BL-21 and induced by IPTG for its expression. The expressed proteins obtained were identified by SDS-PAGE and Western blotting.
  
  
  RESULTS
  The core sequence of HCV was amplified and after IPTG induction, a fusion protein of 32,000 was resulted exhibiting specific reaction with HCV-positive serum and high antigenicity.
  
  
  CONCLUSIONS
  It is possible to efficiently express HCV core protein in E.coli.
authors.affiliation: Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515, China. zhang9680@hotmail.com
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Wen-qi
authors.initials: WQ
authors.lastname: Dong
authors.forename: Ying-song
authors.initials: YS
authors.lastname: Wu
authors.forename: Ze-wei
authors.initials: ZW
authors.lastname: Tian
authors.forename: Xian-bo
authors.initials: XB
authors.lastname: Wu
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D016174:Hepacivirus / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014758:Viral Core Proteins / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014758:Viral Core Proteins