title: Antiproliferative activity of cardenolides isolated from Streptocaulon juventas.
abstract: Sixteen cardenolides, two hemiterpenoids, two phenylpropanoids and a phenylethanoid isolated from the roots of Streptocaulon juventas (LOUR.) MERR. were examined for their antiproliferative activity toward three human-derived (HT-1080 fibrosarcoma, lung A549 adenocarcinoma, cervix HeLa adenocarcinoma) and three murine-derived (colon 26-L5 carcinoma, Lewis lung carcinoma, B16-BL6 melanoma) cell lines. The cardenolides selectively and strongly inhibited proliferation of the HT-1080 (IC(50) values, 0.054-1.6 microM) and A549 (IC(50), 0.016-0.65 microM) cell lines. The characteristic morphological changes and ladder-like DNA fragmentation in those cells treated with the cardenolides indicated the antiproliferative activity was due to the induction of apoptosis.
authors.affiliation: Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Ueda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Arjun Hari
authors.initials: AH
authors.lastname: Banskota
authors.forename: Quan Le
authors.initials: QL
authors.lastname: Tran
authors.forename: Qui Kim
authors.initials: QK
authors.lastname: Tran
authors.forename: Ikuo
authors.initials: I
authors.lastname: Saiki
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kadota
mesh_terms: D000818:Animals; D029062:Apocynaceae; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots
chemical_list: D002298:Cardenolides; D006131:Growth Inhibitors; D010936:Plant Extracts