title: Neisseria meningitidis carriage during an outbreak of serogroup C disease.
abstract: During 2001, an outbreak of serogroup C meningococcal disease led to immunization of individuals aged 13-29 years in Abbotsford, British Columbia, Canada. This study addresses the distribution of Neisseria meningitidis carriage in this population and the implications of that distribution for the targeting of the immunization campaign. Pharyngeal swabs were obtained at immunization from 2004 people. Colonies were identified and serogrouped using standard agglutination methods and by PCR. Isolates were characterized using pulsed-field gel electrophoresis (PFGE). The prevalence of N. meningitidis carriage was 153 carriers per 2004 subjects (7.6%; 95% confidence interval, 6.5%-8.9%). Only 6 (4%) of the isolates from these carriers were found to be serogroup C by agglutination or PCR testing, and all of these were from individuals within the age group targeted for immunization. Only 1 of these 6 isolates was found to be identical to the outbreak strain by PFGE. The observation that a virulent strain is not circulating widely suggests the possibility of low background immunity in the population at risk and emphasizes the importance of vaccination in controlling epidemic group C meningococcal disease.
authors.affiliation: Communicable Disease Epidemiology, University of British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada. david.patrick@bccdc.ca
authors.forename: David M
authors.initials: DM
authors.lastname: Patrick
authors.forename: Sylvie
authors.initials: S
authors.lastname: Champagne
authors.forename: Swee-Han
authors.initials: SH
authors.lastname: Goh
authors.forename: Gillian
authors.initials: G
authors.lastname: Arsenault
authors.forename: Eva
authors.initials: E
authors.lastname: Thomas
authors.forename: Carol
authors.initials: C
authors.lastname: Shaw
authors.forename: Tazim
authors.initials: T
authors.lastname: Rahim
authors.forename: Fatma
authors.initials: F
authors.lastname: Taha
authors.forename: Mark
authors.initials: M
authors.lastname: Bigham
authors.forename: Valerie
authors.initials: V
authors.lastname: Dubenko
authors.forename: Danuta
authors.initials: D
authors.lastname: Skowronski
authors.forename: Robert C
authors.initials: RC
authors.lastname: Brunham
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002353:Carrier State; D002648:Child; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D008297:Male; D008589:Meningococcal Infections / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000008:administration & dosage; D008875:Middle Aged; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D012703:Serotyping; D012737:Sex Factors
chemical_list: D022401:Meningococcal Vaccines