The human pituitary proteome: the characterization of differentially expressed proteins in an adenoma compared to a control.
In order to clarify the basic molecular mechanisms that participate in the formation of human pituitary macroadenomas, this study, for the first time, describes the comparative proteomics between a pituitary adenoma tissue and a control tissue. A vertical, two-dimensional polyacrylamide gel electrophoresis system and PDQuest image analysis software were used to provide a high level of between-gel reproducibility and electrophoretic separation to accurately locate each differentially expressed protein. Mass spectrometry (MALDI-TOF and LC-ESI-Q-IT) and protein databases were used to characterize each differentially expressed protein. A total of 137 differential gel spots (37 increased spot volumes, 39 decreased, 19 new and 42 lost) were found when we compared an adenoma proteome to a control proteome. Seventy-one spots (20 increased, 27 decreased, 13 new, 11 lost), representing 39 differentially regulated proteins, were identified. Five differentially regulated proteins (prolactin, cellular retinoic acid-binding protein II, G-protein beta subunit 3, secretagogin and calreticulin) were also validated with results from a comparative transcriptomics study of pituitary adenomas and controls. The functional characteristics of these differentially expressed proteins provide a differential proteomic profile between a pituitary adenoma and a control.
Desiderio
D M
DM
The Charles B. Stout Neuroscience Mass Spectrometry Laboratory, The University of Tennessee Health Science Center, 847 Monroe Avenue, Room 117, Memphis, TN 38163, USA. ddesiderio@utmem.edu
Zhan
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D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D001330:Electronic Data Processing / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006868:Hydrolysis; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010902:Pituitary Gland / Q000737:chemistry* / Q000473:pathology; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D016622:Silver Staining; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014357:Trypsin / Q000378:metabolism
D020543:Proteome; D014357:Trypsin