Directed migration of neuronal precursors into the ischemic cerebral cortex and striatum.
Pathological processes, including cerebral ischemia, can enhance neurogenesis in the adult brain, but the fate of the newborn neurons that are produced and their role in brain repair are obscure. To determine if ischemia-induced neuronal proliferation is associated with migration of nascent neurons toward ischemic lesions, we mapped the migration of cells labeled by cell proliferation markers and antibodies against neuronal marker proteins, for up to 2 weeks after a 90-min episode of focal cerebral ischemia caused by occlusion of the middle cerebral artery. Doublecortin-immunoreactive cells in the rostral subventricular zone, but not the dentate gyrus, migrated into the ischemic penumbra of the adjacent striatum and, via the rostral migratory stream and lateral cortical stream, into the penumbra of ischemic cortex. These results indicate that after cerebral ischemia, new neurons are directed toward sites of brain injury, where they might be in a position to participate in brain repair and functional recovery.
Jin
Kunlin
K
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA.
Sun
Yunjuan
Y
Xie
Lin
L
Peel
Alyson
A
Mao
Xiao Ou
XO
Batteur
Sophie
S
Greenberg
David A
DA
D000818:Animals; D015415:Biomarkers; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D003342:Corpus Striatum / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development; D000089662:Doublecortin Protein; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D009416:Nerve Regeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013687:Telencephalon / Q000254:growth & development* / Q000503:physiopathology
D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D009419:Nerve Tissue Proteins