Rat behavior in two models of anxiety and brain [3H]muscimol binding: pharmacological, correlation, and multifactor analysis.
The contribution of GABAergic mechanisms to rat emotional behavior in two animal models of anxiety (open field test of neophobia and aversively conditioned freezing reaction), was confirmed by pharmacological analysis, using anxiolytic (midazolam) and anxiogenic (picrotoxin) compounds. Both substances are known to modulate GABA(A) receptors' activity in a positive or negative manner, respectively. It seemed, therefore, worthwhile to check whether the behavioral parameters measured in these animal models of anxiety correlate with [3H]muscimol binding (a highly selective GABA(A) receptor ligand) in different brain structures of nai;ve rats, with a view to establish the role of genetically determined expression of local GABA(A) receptors in the organization of rat emotional and motor behavior. Correlation analysis revealed no links between individually determined expression of GABA(A) receptors (quantitative receptor autoradiography) in the brain structures, and the emotional behavior of nai;ve, drug-free animals, in both tests. Factor analysis confirmed that animal behavior in both tests was under control of different central processes. Moreover, none of the behavioral and ligand binding parameters loaded on the same factor, confirming the negative results of the correlation study. The present results indicate that the origin of emotions is a complex phenomenon, probably involving the interaction between GABA-ergic innervation of many brain structures.
Sienkiewicz-Jarosz
Helena
H
First Department of Neurology, Medical University, Warsaw, Poland.
Szyndler
Janusz
J
Członkowska
Agnieszka I
AI
Siemiatkowski
Marek
M
Maciejak
Piotr
P
Wisłowska
Aleksandra
A
Zienowicz
Małgorzata
M
Lehner
Małgorzata
M
Turzyńska
Danuta
D
Bidziński
Andrzej
A
Płaźnik
Adam
A
D000704:Analysis of Variance; D000818:Animals; D001007:Anxiety / Q000378:metabolism*; D001345:Autoradiography; D001522:Behavior, Animal; D001665:Binding Sites; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004597:Electroshock / Q000009:adverse effects; D005106:Exploratory Behavior / Q000187:drug effects; D005163:Factor Analysis, Statistical; D005239:Fear / Q000187:drug effects; D018755:GABA Agonists / Q000493:pharmacokinetics* / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D008297:Male; D008874:Midazolam / Q000494:pharmacology; D009118:Muscimol / Q000493:pharmacokinetics* / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014316:Tritium / Q000493:pharmacokinetics
D018755:GABA Agonists; D018756:GABA Antagonists; D018757:GABA Modulators; D008874:Midazolam; D009118:Muscimol; D010852:Picrotoxin; D014316:Tritium