Comparison between adsorption isotherm determination techniques and overloaded band profiles on four batches of monolithic columns.
The adsorption isotherms of 4-tert.-butyl phenol were measured on four different monolithic columns, using three different techniques, classical frontal analysis (FA), the perturbation on a plateau method (PP) and the recently introduced numerical procedure known as the inverse numerical method (IN). This last approach requires only the recording of a few overloaded profiles and has the potential advantage of affording a dramatic decrease of the amounts of compounds, solvent, and time needed to determine accurate estimates of the coefficients of the isotherm. The reproducibility of the adsorption data measured on the four columns is discussed with reference to the specific techniques used for obtaining these data and to the most suitable equation used for modeling them. The data obtained for the different columns were highly consistent. The inverse numerical approach was confirmed to provide a powerful, accurate, and economic method for measuring single component adsorption data.
Cavazzini
Alberto
A
Department of Chemistry, University of Tennessee, 552 Buehler Hall, Knoxville, TN 37996-1600, USA.
Felinger
Attila
A
Guiochon
Georges
G
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D008956:Models, Chemical; D015203:Reproducibility of Results; D013816:Thermodynamics