Tri16 is required for esterification of position C-8 during trichothecene mycotoxin production by Fusarium sporotrichioides.
We previously characterized Tri1, a gene required for hydroxylation of the C-8 position during trichothecene mycotoxin biosynthesis in Fusarium sporotrichioides NRRL 3299. Sequence analysis of the region surrounding Tri1 revealed a gene, named Tri16, which could encode an acyltransferase. Unlike the wild-type parent strain NRRL 3299, which accumulates primarily T-2 toxin along with low levels of diacetoxyscirpenol (DAS) and neosolaniol (NEO) and trace amounts of 8-propionyl-neosolaniol (P-NEO) and 8-isobutyryl-neosolaniol (B-NEO), mutants containing a disruption of Tri16 were blocked in the production of the three C-8 esterified compounds T-2 toxin, P-NEO, and B-NEO and accumulated the C-8-hydroxylated compound NEO along with secondary levels of DAS. These data indicate that Tri16 encodes an acyltransferase that catalyzes the formation of ester side groups at C-8 during trichothecene biosynthesis. We also report the presence of a Tri16 ortholog in Gibberella pulicaris R-6380 that is likely linked to a presumably inactive ortholog for Tri1.
Peplow
Andrew W
AW
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843, USA.
Meek
Isaac B
IB
Wiles
Melinda C
MC
Phillips
Timothy D
TD
Beremand
Marian N
MN
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D004951:Esterification; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005874:Gibberella / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013605:T-2 Toxin / Q000096:biosynthesis*; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism
D005656:Fungal Proteins; D014255:Trichothecenes; C016009:neosolaniol; D000217:Acyltransferases; D013605:T-2 Toxin