[Effects of cisplatin and sodium selenite on transformation and rRNA gene transcription activity of human T lymphocytes].
In order to investigate the effect of sodium selenite on the action of cisplatin inhibiting transformation of human T lymphocytes, explore the influence of cisplatin and sodium selenite on rRNA gene transcription activity of lymphocytes and to explore the relationship between transformation of T cells and rRNA gene transcription activity, cisplatin was administered to the culture of lymphocytes after incubation for 24 hours with three different dosages (0.05, 0.2, and 0.5 mg/L). The culture was also supplemented with sodium selenite (0.05 mg/L) at the beginning of the cell culture or at the same time as cisplatin was administered for different treatment. The slide specimens were prepared using cytogenetical method after the cells were cultured for 72 hours, and then, the changes of the transformation rate of T lymphocytes and frequencies of Ag-NORs that reflect rRNA gene transcription activity were investigated. The results showed that the rate of T lymphocyte transformation in the groups treated with both sodium selenite and cisplatin were significantly increased (p < 0.01) as compared with that in the groups without sodium selenite supplementation and suffering exposures to cisplatin alone at corresponding dose, up to or over the rate of negative control group. There were no significant differences in the ratios of lymphocyte transformation between the groups pre-treated by sodium selenite and the groups treated by both sodium selenite and cisplatin on the condition that the other treatments were the same. There were no remarkable differences (p > 0.05) in the frequencies of Ag-NOR and satellite association among the all groups. This study indicated that sodium selenite supplementation can preclude the inhibition of transformation of T lymphocytes in vitro by cisplatin, regardless the pre-treatment by sodium selenite or co-treatment with cisplatin.
Chen
Xianjun
X
Biology Department, Xianning Medical College, Xianning 437100, China.
Zhao
Honggang
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D012335:RNA, Ribosomal / Q000235:genetics*; D018038:Sodium Selenite / Q000494:pharmacology*; D014158:Transcription, Genetic
D000970:Antineoplastic Agents; D012335:RNA, Ribosomal; D018038:Sodium Selenite; D002945:Cisplatin