[The role of protein kinase C in Na+ transport regulation in the skin of adult frogs and tadpoles of Rana temporaria].
Using the voltage-clamp technique, a possible role of protein kinase C in regulation of Na+ transport in the skin of the frog Rana temporaria was investigated. It was shown that protein kinase C activator phorbol ester 12-myristate 13-acetate (PMA), applied to the apical surface of the skin, stimulated transepithelial Na+ transport, measured as amiloride-sensitive short-circuit current, and also increased such electrical characteristics of frog skin as the open-circuit potential and transepithelial conductance. PMA exerts a similar stimulation effect on Na+ transport across the tadpole skin. Specific inhibitors of protein kinase C, chelerythryne or H-7, almost fully prevented the PMA-induced stimulation of Na+ transport. These data support a concept that the response to PMA was indeed mediated by PKC activation. The results are compatible with the important role played by protein kinase C in regulation of transepithelial Na+ transport in the skin of R. temporaria.
Krutetskaia
Z I
ZI
St. Petersburg State University.
Lebedev
O E
OE
Pashina
A V
AV
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology / Q000378:metabolism; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D007814:Larva; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011896:Rana temporaria; D012867:Skin / Q000201:enzymology / Q000378:metabolism*; D012879:Skin Physiological Phenomena; D012964:Sodium / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology
D004791:Enzyme Inhibitors; C016658:phorbolol myristate acetate; D000584:Amiloride; D012964:Sodium; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate