title: Detrimental effects of N(omega) nitro-L-arginine methyl ester (L-NAME)in experimental Escherichia coli sepsis in the newborn piglet.
abstract: The role of nitric oxide during neonatal sepsis is complex. We tested the hypothesis that nonselective inhibition of nitric oxide synthase with N(omega) -nitro-L-arginine methyl ester (L-NAME) is detrimental during the early phase of experimental sepsis in the newborn piglet. Newborn piglets were divided into four groups: 6 in the control group, 6 in the L-NAME control group, 12 in the sepsis group (SG), and 11 in the sepsis with L-NAME group (NS). Sepsis was induced by intravenous injection of 10(8) colony forming units of Escherichia coli. L-NAME 10 mg/kg was given intravenously 60 min before the induction of sepsis. The survival rate of piglets after 4 hr was 27% in NS, while it was 100% in other groups. Systemic hypotension, observed in both SG and NS, were more profound in NS. Leukopenia was observed in both SG and NS. Thrombocytopenia, prolongation of prothrombin time and activated partial thromboplastin time, and increase in thrombin-antithrombin complexes were observed only in NS. Decreased PaO2 /FiO2 ratio, arterial pH and base excess, and increased blood lactate levels observed in both SG and NS, but were more profound in NS. These findings suggest that nonselective inhibition of nitric oxide synthase with L-NAME is detrimental during the early phase of experimental neonatal sepsis.
authors.affiliation: Department of Pediatrics, Bucheon Hospital, Soonchunhyang University, College of Medicine,Bucheon, Korea.
authors.forename: Sung Shin
authors.initials: SS
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Hwang
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Shim
authors.forename: Yun Sil
authors.initials: YS
authors.lastname: Chang
authors.forename: Won Soon
authors.initials: WS
authors.lastname: Park
authors.forename: Chang Kyu
authors.initials: CK
authors.lastname: Oh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D018805:Sepsis / Q000188:drug therapy*; D013552:Swine; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D019331:NG-Nitroarginine Methyl Ester