Gastric smooth muscle contractility changes in the esophageal atresia rat model: an in vitro study.
OBJECTIVE
The aim of the study was to investigate the gastric smooth muscle reactivity in the Adriamycin-induced esophageal atresia (EA) rat model.


METHODS
The fetuses were divided into 3 groups. The control group was exposed to saline. The second group was comprised of fetuses that were exposed to Adriamycin but did not have EA (Adriamycin-no-EA group). The third group was comprised of fetuses that were exposed to Adriamycin and had EA (Adriamycin-EA group). Gastric fundus strips were studied in vitro for their contractile response to receptor activation in the 3 groups.


RESULTS
Contractile responses of gastric smooth muscle to carbachol and KCl were increased in the Adriamycin-EA group compared with the Adriamycin-no-EA group. Also serotonin-induced contractile response in the Adriamycin-EA group decreased compared with the Adriamycin-no-EA group. Relaxation of gastric smooth muscle strips to isoproterenol was comparably unaffected in the Adriamycin-EA and Adriamycin-no-EA groups. Likewise, no change in the response to agonist studies was observed between the control and Adriamycin-no-EA groups. The relaxant response to papaverine was not different in the 3 groups.


CONCLUSIONS
This study found changes of receptor-dependent and receptor-independent contraction of the gastric fundus smooth muscle in the fetuses with EA. Therefore, impaired contractile responses may be, at least in part, a contributing factor in the abnormal gastric motility seen in EA.
Tugay
Melih
M
Kocaeli University, Istanbul, Turkey.
Yildiz
Fürüzan
F
Utkan
Tijen
T
Sarioglu
Yusuf
Y
Gacar
Nejat
N
D000818:Animals; D004195:Disease Models, Animal; D004317:Doxorubicin; D004933:Esophageal Atresia / Q000139:chemically induced / Q000503:physiopathology*; D005333:Fetus; D005748:Gastric Fundus / Q000503:physiopathology*; D066298:In Vitro Techniques; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004317:Doxorubicin