title: Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance.
abstract: RNA transcript levels of Arabidopsis plants, infected by the rhizobacterium Pseudomonas thivervalensis (strain MLG45), and axenic control plants were compared using cDNA microarrays representing approximately 14 300 genes. The analysis revealed an increase of defence-related transcripts in the shoots of bacterized plants relative to control (axenic) plants. These modifications of transcript levels were confirmed by physiological experiments. Plants infected with P. thivervalensis were more resistant to subsequent infections by the virulent pathogen P. syringae pv. tomato (strain DC3000) than control plants. In addition, photosynthesis rates were repressed consistently with the reduced growth of plants colonized by P. thivervalensis. These results highlight the value of molecular phenotyping to predict physiological changes.
authors.affiliation: Laboratoire de Biologie du Développement des Plantes, UMR CNRS-CEA-University méditerranée 163, DSV.DEVM, CEA/Cadarache, F-13108 Saint Paul lez Durance Cedex, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Cartieaux
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Thibaud
authors.forename: Laurent
authors.initials: L
authors.lastname: Zimmerli
authors.forename: Philippe
authors.initials: P
authors.lastname: Lessard
authors.forename: Catherine
authors.initials: C
authors.lastname: Sarrobert
authors.forename: Pascale
authors.initials: P
authors.lastname: David
authors.forename: Alain
authors.initials: A
authors.lastname: Gerbaud
authors.forename: Christophe
authors.initials: C
authors.lastname: Robaglia
authors.forename: Shauna
authors.initials: S
authors.lastname: Somerville
authors.forename: Laurent
authors.initials: L
authors.lastname: Nussaume
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D018533:Biomass; D002245:Carbon Dioxide / Q000032:analysis; D017931:DNA Primers; D007113:Immunity, Innate / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010838:Phytophthora / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D002245:Carbon Dioxide