Acetylcholinesterase activity and molecular isoform distribution are altered after focal cerebral ischemia.
Biochemical changes in rat brain acetylcholinesterase (AChE) activity and molecular isoform distribution were studied using the middle cerebral artery occlusion model. After 24 h of permanent focal ischemia, AChE specific activity from infarcted cortex was found to decrease compared with contralateral cortex. Also a decrease in the minor monomeric (G1) form of AChE is response to ischemia was observed. Ischemia-induced AChE decrease was partially diminished by pre-treatment with dizocilpine (MK-801), a neuroprotective NMDA receptor blocker.
Sáez-Valero
Javier
J
Instituto de Neurociencias, Universidad Miguel Hernández, San Juan de Alicante, Spain.
González-García
Carmen
C
Ceña
Valentín
V
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D016291:Dizocilpine Maleate / Q000494:pharmacology / Q000627:therapeutic use; D007839:Functional Laterality; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D020033:Protein Isoforms / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D018696:Neuroprotective Agents; D020033:Protein Isoforms; D016291:Dizocilpine Maleate; D000110:Acetylcholinesterase