Angiotensin converting enzyme inhibitory phenylpropanoid glycosides from Clerodendron trichotomum.
The stems of Clerodendron trichotomum have been traditionally used for treatment of hypertension in far East Asia including China, Korea, and Japan. Bioassay-guided fractionation and purification of the EtOAc-soluble extract of Clerodendron trichotomum afforded acteoside (1), leucosceptoside A (2), martynoside (3), acteoside isomer (4), and isomartynoside (5). The angiotensin converting enzyme (ACE) activities were significantly inhibited by the addition of these phenylpropanoid glycosides (1-5) in a dose-dependent manner of which IC(50) values were 373+/-9.3 microg/ml, 423+/-18.8 microg/ml, 524+/-28.1 microg/ml, 376+/-15.6 microg/ml, 505+/-26.7 microg/ml, respectively. These results suggest that the antihypertensive effect of Clerodendron trichotomum may be, at least in part, due to ACE inhibitory effect of phenylpropanoid glycosides.
Kang
Dae Gill
DG
Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeon-Buk 570-749, South Korea.
Lee
Yong Sup
YS
Kim
Hyoung Ja
HJ
Lee
Yun Mi
YM
Lee
Ho Sub
HS
D000085:Acetates; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D032409:Clerodendrum; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems; D010946:Plants, Medicinal; D011422:Propionates / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
D000085:Acetates; D000806:Angiotensin-Converting Enzyme Inhibitors; D006027:Glycosides; D010936:Plant Extracts; D011422:Propionates; C007650:ethyl acetate