title: Molecular evidence supporting the classification of Hosta virus X as a distinct species of the genus.
abstract: Apotexvirus, Hosta virus X (HVX-Kr), causing mosaic and mottle symptoms was isolated from hosta plants ( Hosta spp.) in Korea. The 3'-terminal 2,711 nucleotides excluding the poly (A) tail were determined and shown to include the partial viral replicase, triple gene block (TGB) 1 (26 kDa), TGB2 (13 kDa), TGB3 (8 kDa), and 23 kDa coat protein (CP) and the 3'-nontranslated region (NTR), typical of potexviruses. The CP gene of the type isolate of HVX (HVX-U) was amplified by RT-PCR and its nucleotide sequence was determined. The CPs of HVX-Kr and HVX-U had 100% and 98.9% identical amino acids and nucleotides, respectively. Most of the regions of the genome HVX had over 50% nucleotide identical to other sequenced potexviruses. This is the first report of sequence information of HVX and molecular evidence supporting the virus as a distinct species of the genus Potexvirus.
authors.affiliation: Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University, Seoul, Republic of Korea.
authors.forename: M H
authors.initials: MH
authors.lastname: Park
authors.forename: K H
authors.initials: KH
authors.lastname: Ryu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D031422:Hosta / Q000821:virology*; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D020413:3' Untranslated Regions