title: Interaction between gfp-tagged Pseudomonas tolaasii P12 and Pleurotus eryngii.
abstract: Pseudomonas sp., (formerly reported as strain P12) which produces brown blotch disease symptoms on Pleurotus eryngii, has been identified as P. tolaasii based on its biochemical, physiological properties and 16S rDNA sequence analysis. This pathogen is able to infect basidiocarps when surface-inoculated on mushroom casing soil. However, infected basidiocarps develop the brown blotch disease symptoms when the pathogen concentration in the fruiting body tissues is higher than 10(4) cfu/g d.w. Using gfp-tagged cells and confocal laser scanning microscopy, it was possible to show that the pathogen has the ability to tightly attach to the hyphae of Pleurotus eryngii.
authors.affiliation: Università di Pisa, Dipartimento di Chimica e Biotecnologie Agrarie, via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Russo
authors.forename: Carlo
authors.initials: C
authors.lastname: Filippi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Tombolini
authors.forename: Annita
authors.initials: A
authors.lastname: Toffanin
authors.forename: Stefano
authors.initials: S
authors.lastname: Bedini
authors.forename: Monica
authors.initials: M
authors.lastname: Agnolucci
authors.forename: Marco
authors.initials: M
authors.lastname: Nuti
mesh_terms: D001422:Bacterial Adhesion; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004275:DNA, Ribosomal / Q000737:chemistry; D017930:Genes, Reporter; D016680:Genome, Bacterial; D049452:Green Fluorescent Proteins; D025301:Hyphae / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D025282:Mycelium / Q000502:physiology; D020076:Pleurotus / Q000166:cytology / Q000502:physiology*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins