Superparamagnetic iron oxide particles transactivator protein-fluorescein isothiocyanate particle labeling for in vivo magnetic resonance imaging detection of cell migration: uptake and durability.
Conjugation of dextran-coated superparamagnetic iron oxide (SPIO) particles with transactivator protein (Tat)-peptide and fluorescein isothiocyanate (FITC) allows cells to readily uptake SPIO particles. This makes possible high-resolution, real-time imaging of these cells by magnetic resonance imaging (MRI). First, we need to understand how various subpopulations take up and maintain SPIO particles. In this report, we have focused on differences in T cells, B cells, and macrophages with respect to cross-linked (CL)-SPIO Tat-FITC particle uptake over 72 hours. We have found that cells quickly take up the particles and that the bead loss that does occur is not related to cell death or apoptosis. In contrast with reports in the literature, we have observed migration of the Tat-peptide conjugates primarily to the cytoplasm rather than the nucleus.
Kaufman
Christina L
CL
Institute for Cellular Therapeutics, Department of Surgery, University of Louisville, Louisville, KY, USA. clkauf01@Louisville.edu.
Williams
Mangay
M
Ryle
L Madison
LM
Smith
Traci L
TL
Tanner
Mike
M
Ho
Chien
C
D000818:Animals; D002465:Cell Movement; D003432:Cross-Linking Reagents; D003911:Dextrans; D004355:Drug Stability; D005260:Female; D052203:Ferrosoferric Oxide; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000493:pharmacokinetics; D015694:Gene Products, rev / Q000737:chemistry / Q000493:pharmacokinetics; D007501:Iron / Q000737:chemistry / Q000493:pharmacokinetics; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010087:Oxides / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*
D003432:Cross-Linking Reagents; D003911:Dextrans; D015694:Gene Products, rev; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; D007501:Iron; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide