title: Fluorescence visualization of ultraviolet-sensitive cone photoreceptor development in living zebrafish.
abstract: Cone photoreceptor cells of fish retinae are arranged in a highly organized fashion. However, the molecular mechanisms underlying photoreceptor development and retinal pattern formation are largely unknown. Here we established transgenic lines of zebrafish carrying green fluorescent protein (GFP) cDNA with the 5.5-kb upstream region of the ultraviolet-sensitive cone opsin gene (SWS1). In the transgenic fish, GFP gene expression proceeded in the same spatiotemporal pattern as SWS1 in the retinae of embryos. In the adult retina, GFP expression was observed throughout the short single cone (SSC) layer where SWS1 is specifically expressed. Therefore, the transgenic fish provides an excellent genetic background to study retinal pattern formation, photoreceptor determination and differentiation, and factors regulating these processes and SSC-specific expression of SWS1.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Takechi
authors.forename: Takanori
authors.initials: T
authors.lastname: Hamaoka
authors.forename: Shoji
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002454:Cell Differentiation; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000196:embryology* / Q000378:metabolism* / Q000528:radiation effects; D017299:Rod Opsins / Q000235:genetics*; D019076:Transgenes / Q000235:genetics; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D017299:Rod Opsins; D049452:Green Fluorescent Proteins