title: The effect of prophylactic melatonin administration on reperfusion damage in experimental testis ischemia-reperfusion.
abstract: OBJECTIVE
  Torsion of testis, which is a urologic emergency case, is generally treated by surgical detorsion procedure. However, the resulting reperfusion and both ipsilateral and contralateral testis damage caused thereby are important problems. This study aims at investigating the administration of prophylactic melatonin in order to reduce free radical damage that is caused due to reperfusion after experimental testis torsion-detorsion procedure.
  
  
  METHODS
  The rats used in the study were allocated to four groups, each containing 10 rats. Rats in Group I had six hours of torsion (ischemia) followed by orchiectomy. Rats in Group II had six hours of torsion, then received melatonin (10 mg/kg/IM) and after that had detorsion (reperfusion). Rats in Group III had detorsion after six hours of torsion and serum physiologic administration (the same volume as melatonin). Torsion and/or detorsion procedures were not applied in Group IV (control). Ipsilateral, contralateral testis and plasma Malondialdehyde (MDA) levels were determined in all groups.
  
  
  RESULTS
  Tissue and plasma MDA levels in the group which had detorsion were found to be significantly higher than those in the group that had orchiectomy only (p<0.02). Ipsilateral and contralateral testis MDA levels were identified to be significantly lower in the group receiving prophylactic melatonin in comparison to the group receiving serum physiologic (p<0.02). Plasma and contralateral MDA levels correlated positively with MDA levels identified in ipsilateral testis in all groups (rs=+0.89, n=40, p=0.000).
  
  
  CONCLUSIONS
  In cases where testis torsion is identified, administration of melatonin just before torsion may reduce local and systemic free radical damage.
authors.affiliation: Selcuk University, School of Medicine, Urology Department, Konya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozturk
authors.forename: Abdulkerim Kasim
authors.initials: AK
authors.lastname: Baltaci
authors.forename: Rasim
authors.initials: R
authors.lastname: Mogulkoc
authors.forename: Bahadir
authors.initials: B
authors.lastname: Ozturk
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008550:Melatonin / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000473:pathology; D013733:Testicular Diseases / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008550:Melatonin