Use of Xenopus oocytes to study the expression of cloned genes and translation of mRNA.
One of the most active areas of research in the field of molecular biology is the examination of the mechanisms associated with the regulation of gene expression. Our understanding of the events in eukaryotic transcription has been aided by the ability to test the expression of various genomic DNA constructs after their microinjection into the germinal vesicle of Xenopus oocytes. This in vivo transcription system has permitted the analysis of the involvement of cis-acting DNA sequences and the examination of the effect of co-injected trans-acting factors on gene expression. Furthermore, the Xenopus oocyte has been employed widely as an in vivo translation system. Not only is the Xenopus oocyte system a sensitive assay for the translation of rare mRNAs but it also has the ability to post-translationally modify and compartmentalize numerous types of proteins. Finally, the Xenopus oocyte has proven useful in the procedures associated with the cloning and screening of cDNAs.
Heikkila
J J
JJ
Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada.