title: Transabdominal near infrared oximetry of hypoxic stress in fetal sheep brain in utero.
abstract: The feasibility of transabdominal near-infrared (NIR) spectroscopy for detecting and quantifying fetal hypoxia in utero is demonstrated in a pregnant ewe model. A frequency domain NIR spectroscopy probe, consisting of two detectors and six sources operating at three wavelengths (675, 786, and 830 nm), was placed on the maternal abdomen directly above the fetal head. Fetal hypoxia was indirectly induced through occlusion of uterine blood flow for approximately 3 min. NIR photon diffusion measurements were made during a baseline period, during hypoxia of the fetus, and during recovery. Fetal blood samples were drawn from the fetal brachial artery and jugular veins at several time points during the cycle. Seven hypoxic cycles were induced in a total of five pregnant ewes. The NIR measurements were analyzed by using a two-layer diffusion model to deconvolve the fetal blood saturation from that of the pregnant ewe. Fetal hypoxia was detected. Good agreement was found between fetal blood saturation determined by the transabdominal NIR method and arterial and venous fetal blood saturation quantified from fetal blood samples by using a hemoximeter.
authors.affiliation: Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA. rgchoe@mail.sas.upenn.edu
authors.forename: Regine
authors.initials: R
authors.lastname: Choe
authors.forename: Turgut
authors.initials: T
authors.lastname: Durduran
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Yu
authors.forename: Mark J M
authors.initials: MJ
authors.lastname: Nijland
authors.forename: Britton
authors.initials: B
authors.lastname: Chance
authors.forename: Arjun G
authors.initials: AG
authors.lastname: Yodh
authors.forename: Nirmala
authors.initials: N
authors.lastname: Ramanujam
mesh_terms: D000005:Abdomen; D000818:Animals; D001921:Brain; D002560:Cerebrovascular Circulation; D004867:Equipment Design; D000860:Hypoxia / Q000097:blood*; D023421:Models, Animal; D010092:Oximetry / Q000295:instrumentation* / Q000379:methods*; D012756:Sheep; D013055:Spectrophotometry, Infrared