Purification and structural characterization of lectins from the cnidarian Bunodeopsis antilliensis [corrected].
Purification and characterization of two different lectins from the Mexican anemone Bunodeopsis antilliensis are reported. These two lectins named Bunodeopsis antilliensis agglutinin-A (BAA-A) and -B (BAA-B) presented the following characteristics: BAA-A was resolved as a component, with haemagglutinating activity for human blood type A (N-acetylgalactosamine-galactose-fucose), with a molecular weight of 28,900 obtained by means of mass spectrometry, showed an isoelectric point of 5.04 with a higher carbohydrate specificity for N-acetylgalactosamine (GalNAc). The analysis of the N-terminal revealed it is related to phosphoesterase and GTP binding protein. BAA-B mainly active with human blood type B (galactose-galactose-fucose) was resolved into three fractions (BAA-B1-3). Their molecular weight were: BAA-B(1) 39,350, BAA-B(2) 28,300 and BAA-B(3) 17,550. The estimated isoelectric points were 8.05, 4.66 and 6.60, respectively. Only fraction 3 exhibited haemagglutinating activity with a higher carbohydrate specificity for galactose and mannose. The analysis of the N-terminal pointed out it is related with phospholipase A(2). We suggest these lectins could be related to a feeding strategy[corrected].
Fenton-Navarro
Bertha
B
Jefatura de División de Estudios de Posgrado, Facultad de Medicina Dr Ignacio Chávez, UMSNH, Dr Rafael Carrillo esq, Dr Salvador Gonzalez Herrejón, Col Bosque-Cuauhtemoc, Centro CP 58000, Morelia, Michocán 5800, Mexico. bertha00_mx@yahoo.com
García-Hernández
Enrique
E
Heimer
Edgar
E
Aguilar
Manuel B
MB
Rodríguez-A
Claudia
C
Arreguín-Espinosa
Roberto
R
Falcón
Andrés
A
D000595:Amino Acid Sequence; D000818:Animals; D003063:Cnidaria / Q000737:chemistry*; D006384:Hemagglutination; D006388:Hemagglutinins / Q000737:chemistry / Q000502:physiology; D007526:Isoelectric Point; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid
D006388:Hemagglutinins; D037102:Lectins; C482094:agglutinin-A; C482095:agglutinin-B