title: Brain tissue and sagittal sinus pO2 measurements using the lifetimes of oxygen-quenched luminescence of a ruthenium compound.
abstract: The study was done to assess the performance of a system that measures the partial pressures of oxygen (pO2) from the lifetimes of oxygen-quenched luminescence of ruthenium compounds immobilized at the tip of fiber-optic optodes (Oxylite system). The system was used to measure the pO2 in brain tissue (thalamus and hypothalamus) and in the sagittal sinus of isoflurane-anesthetized rats at different FiO2's. The pO2 recorded in the hypothalamus (HPtO2) was consistently higher than the pO2 in the thalamus (TPtO2) at all FiO2. HPtO2 was closely related to PvO2 during normoxia but not during hypoxia. The equilibrium time of Oxylite system was found to be rapid compared to in vivo tissue response to changes in FiO2.
authors.affiliation: Biomedical NMR Laboratory, EPR Research Center, Department of Radiology, Dartmouth Medical School, Hanover, NH 03755, USA.
authors.forename: Casmiar I
authors.initials: CI
authors.lastname: Nwaigwe
authors.forename: Marcie A
authors.initials: MA
authors.lastname: Roche
authors.forename: Oleg
authors.initials: O
authors.lastname: Grinberg
authors.forename: Jeff F
authors.initials: JF
authors.lastname: Dunn
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002138:Calibration; D008163:Luminescent Measurements; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017975:Ruthenium Compounds / Q000737:chemistry*
chemical_list: D017975:Ruthenium Compounds; D010100:Oxygen