Labeling during cleavage of nucleic acids for their detection on DNA chips.
A new and efficient strategy for labeling of nucleic acids prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the terminal phosphates of cleaved DNA and RNA fragments with a reporter molecule bearing aryldiazomethane group.
Laayoun
A
A
BioMérieux, Advanced Technology, Chemin de l'Orme, Marcy l'Etoile, France. ali.laayoun@eu.biomerieux.com
Sothier
I
I
Menou
L
L
Bernal-Méndez
E
E
Kotera
M
M
Bourget
C
C
Trévisiol
E
E
Lhomme
J
J
Troesch
A
A
D004247:DNA / Q000737:chemistry*; D006868:Hydrolysis; D007202:Indicators and Reagents; D008968:Molecular Conformation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000737:chemistry*
D007202:Indicators and Reagents; D012313:RNA; D004247:DNA