[Methanogenic sarcina from an anaerobic microbial community degrading p-toluene sulfonate].
The methanogenic strain MM isolated from an anaerobic microbial community degrading p-toluene sulfonate showed optimal values of temperature and pH for growth equal to 37 degrees C and 6.3-6.9, respectively. The doubling times of the isolate grown on methanol, acetate, and methylamines under the optimal conditions were 8.8, 19.1, and 10.3-28.1 h, respectively. The growth of strain MM was observed only when the cultivation medium contained casamino acids or p-toluene sulfonate. The G + C content of the DNA of the isolate was 40.3 mol%. This, together with DNA-DNA hybridization data, allowed the new isolate to be identified as a strain of the species Methanosarcina mazei. The new isolate differed from the known representatives of this species in that it was resistant to alkylbenzene sulfonates and able to demethylate p-toluene sulfonate when grown on acetate.
Shcherbakova
V A
VA
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia. shcherb@ibpm.serpukhov.su
Laurinavichus
K S
KS
Lysenko
A M
AM
Suzina
N E
NE
Akimenko
V K
VK
D000085:Acetates; D000596:Amino Acids; D000693:Anaerobiosis; D001482:Base Composition; D001557:Benzenesulfonates; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media; D019641:DNA, Archaeal / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D017020:Methanosarcina / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008744:Methylamines; D013045:Species Specificity; D013696:Temperature; D014105:Tosyl Compounds / Q000378:metabolism*
D000085:Acetates; D000596:Amino Acids; D001557:Benzenesulfonates; D003470:Culture Media; D019641:DNA, Archaeal; D008744:Methylamines; D014105:Tosyl Compounds; C017721:casamino acids; C029501:4-toluenesulfonic acid; D000432:Methanol