Antimicrobial resistance in Campylobacter from broilers: association with production type and antimicrobial use.
The isolation and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli strains from broilers arriving in French slaughterhouses, were analysed according to production types (i.e. standard, export or free-range) and antimicrobial (i.e. coccidiostats, growth promoters or therapeutic agents) administration in flocks. Prevalence was 56.6% in standard, 51.3% in export and 80.0% in free-range broilers. Three hundred and ninety-three strains were identified. Two-thirds of the strains belonged to the species C. jejuni. The others were C. coli. Antimicrobial susceptibility testing was carried out for ampicillin, nalidixic acid, enrofloxacin, tetracycline, erythromycin and gentamicin according to a dilution method. The percentages of resistant strains were, 23, 25, 17, 57, 0.3 and 0% for C. jejuni and 29, 43, 40, 70, 31 and 0% for C. coli. Statistical analysis revealed significant difference in distribution of C. jejuni and C. coli and antimicrobial resistance according to production type or antimicrobial administration.
Avrain
Laetitia
L
Unité de Mycoplasmologie-Bactériologie, Agence française de Sécurité Sanitaire des Aliments, BP 53, F-22440 Ploufragan, France.
Humbert
Florence
F
L'Hospitalier
Rolande
R
Sanders
Pascal
P
Vernozy-Rozand
Christine
C
Kempf
Isabelle
I
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D017000:Campylobacter coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D000077422:Enrofloxacin; D004917:Erythromycin / Q000494:pharmacology; D024841:Fluoroquinolones / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009268:Nalidixic Acid / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D015363:Quinolones / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D005839:Gentamicins; D015363:Quinolones; D009268:Nalidixic Acid; D000077422:Enrofloxacin; D004917:Erythromycin; D000667:Ampicillin; D013752:Tetracycline