Expressing patterns of p16 and CDK4 correlated to prognosis in colorectal carcinoma.
OBJECTIVE
To describe the correlation between immunostaining patterns of p16 and CDK4 and prognosis in colorectal carcinoma.


METHODS
Paraffin sections of 74 cases of colorectal carcinoma were analysed immunohistochemically for expression of p16 and CDK4 proteins.


RESULTS
Most carcinomas showed stronger p16 and CDK4 immunostaining in the cytoplasm than the adenomas or the adjacent normal mucosa. Strong immunostaining of p16 was a predictor for better prognosis whereas strong cytoplasmic immunostaining of CDK4 was a predictor for poor prognosis. Both p16 and CDK4 immunostainings were correlated with histological grade or Dukes' stage.


CONCLUSIONS
These results support the experimental evidence that interaction of expression of p16 and CDK4 may play an important role in the Rb/p16 pathway, and the expression patterns of CDK4 and p16 may be imperative in the development of colorectal carcinoma, thus becoming a new prognostic marker in colorectal cancer.
Zhao
Po
P
Department of Pathology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China. zhaopo@plagh.com.cn
Hu
Ying-Chuan
YC
Talbot
Ian C
IC
D000236:Adenoma / Q000401:mortality* / Q000473:pathology* / Q000503:physiopathology; D014408:Biomarkers, Tumor; D015179:Colorectal Neoplasms / Q000401:mortality* / Q000473:pathology* / Q000503:physiopathology; D051358:Cyclin-Dependent Kinase 4; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D019175:DNA Methylation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D011237:Predictive Value of Tests; D011379:Prognosis; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000032:analysis; D015996:Survival Rate
D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases