title: Use of immunohistochemistry to diagnose chytridiomycosis in dyeing poison dart frogs (Dendrobates tinctorius).
abstract: Chytridiomycosis, caused by Batrachochytrium dendrobatidis, is an emerging disease of both wild and captive amphibians, posing a threat to their survival in many parts of the world. As the disease can be difficult to diagnose on routine pathologic sections, the purpose of this study was to develop an additional method for visualization. To accomplish this, immunohistochemical staining was applied to histologic skin sections from four experimentally infected Dyeing poison dart frogs (Dendrobates tinctorius). Staining of the positive tissue sections was distinct and readily visualized, making this technique a valuable ancillary diagnostic test for this important disease.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Van Ells
authors.forename: James
authors.initials: J
authors.lastname: Stanton
authors.forename: Ann
authors.initials: A
authors.lastname: Strieby
authors.forename: Peter
authors.initials: P
authors.lastname: Daszak
authors.forename: Alex D
authors.initials: AD
authors.lastname: Hyatt
authors.forename: Corrie
authors.initials: C
authors.lastname: Brown
mesh_terms: D000818:Animals; D001001:Anura; D008411:Chytridiomycota; D003881:Dermatomycoses / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D012867:Skin / Q000382:microbiology / Q000473:pathology