Effects of fludioxonil and pyrimethanil, two fungicides used against Botrytis cinerea, on carbohydrate physiology in Vitis vinifera L.
In Vitis vinifera L, photosynthesis and photosynthate partitioning are affected in the presence of fludioxonil and pyrimethanil, two fungicides commonly used in vineyards against Botrytis cinerea Pers. However, the effects were found to be different according to the model studied: plantlets (cv Chardonnay) grown in vitro, fruiting cuttings (cv Chardonnay) and plants grown in vineyards (cvs Chardonnay, Pinot noir and Pinot Meunier). In the plantlets grown in vitro, both fungicides decreased gas exchanges, photosynthetic pigment and starch concentrations in the leaves, whereas soluble carbohydrates transiently accumulated, suggesting that plantlets mobilised starch in response to photosynthesis inhibition caused by fungicides. In the fruiting cuttings, the fungicides did not affect photosynthesis, although fludioxonil caused starch decrease in parallel with sucrose accumulation, suggesting that the fungicide effects were of lower intensity than in vitro. Conversely, in vineyard, the two fungicides stimulated photosynthesis and increased pigment concentrations in the three vine cultivars tested. In the meantime, glucose, fructose and starch levels of the leaves declined after fungicide exposure, whereas sucrose accumulated, indicating that sucrose synthesis increased in the leaves following the fungicide treatment. Among the three varieties, Chardonnay was the most sensitive to the fungicides as revealed by the intensity of the responses and the longer period for recovery. In vineyard, the results suggested that the two fungicides, in addition to inhibiting B cinerea development, had a beneficial effect on vine physiology through the stimulation of leaf carbon nutrition, which may further enable the plant to rapidly make use of its defence reactions.
Saladin
Gaëlle
G
Laboratoire de Biologie et Physiologie Végétales, URWC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, Moulin de la Housse, BP 1039, F-51687 Reims 2, France.
Magné
Christian
C
Clément
Christophe
C
D020171:Botrytis / Q000187:drug effects*; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis; D002734:Chlorophyll / Q000096:biosynthesis; D004149:Dioxoles / Q000633:toxicity*; D005632:Fructose / Q000378:metabolism; D005659:Fungicides, Industrial / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D011743:Pyrimidines / Q000633:toxicity*; D011758:Pyrroles / Q000633:toxicity*; D013213:Starch / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D027843:Vitis / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology
D004149:Dioxoles; D005659:Fungicides, Industrial; D011743:Pyrimidines; D011758:Pyrroles; D002734:Chlorophyll; D002245:Carbon Dioxide; D005632:Fructose; D002338:Carotenoids; D013395:Sucrose; C108337:pyrimethanil; D013213:Starch; C108339:fludioxonil; D005947:Glucose