Identification of rpoE and nadB as host responsive elements of Yersinia enterocolitica.
We describe the identification of the Yersinia enterocolitica rpoE gene, which encodes the alternative sigma factor sigmaE, and the divergently transcribed nadB gene, as genes that are expressed during infection of mice. As in Escherichia coli, rpoE of Y. enterocolitica is essential for growth and its expression is autoregulated. Despite the similarities of the rpoE operons of Y. enterocolitica and E. coli, there are considerable differences in the response to extracytoplasmic stress. Unlike in E. coli, sigmaE of Y. enterocolitica is not induced by heat shock or ethanol. Overproduction of the outer membrane protein Ail does not lead to the induction of rpoE expression. However, rpoE expression is induced by osmotic stress.
Heusipp
Gerhard
G
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA. heusipp@uni-muenster.de
Schmidt
M Alexander
MA
Miller
Virginia L
VL
D000594:Amino Acid Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D000431:Ethanol / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020043:Genes, Essential; D017930:Genes, Reporter; D018869:Heat-Shock Response / Q000235:genetics; D051379:Mice; D009876:Operon / Q000235:genetics; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D001616:beta-Galactosidase / Q000378:metabolism
D029968:Escherichia coli Proteins; D012808:Sigma Factor; D014157:Transcription Factors; D037521:Virulence Factors; C063202:sporulation-specific sigma factors; D000431:Ethanol; D000594:Amino Acid Oxidoreductases; C031955:L-aspartate oxidase, E coli; D001616:beta-Galactosidase