title: [The extracellular proteases of the phytopathogenic bacterium Xanthomonas campestris].
abstract: The culture liquids of three Xanthomonas campestris pv. campestris strains were found to possess proteolytic activity. The culture liquid of strain B-611 with the highest proteolytic activity was fractionated by salting-out with ammonium sulfate, gel filtration, and ion-exchange chromatography. The electrophoretic analysis of active fractions showed the presence of two proteases in the culture liquid of strain B-611, the major of which being serine protease. The treatment of cabbage seedlings with the proteases augmented the activity of peroxidase in the cabbage roots by 28%.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Kalashnikova
authors.forename: M P
authors.initials: MP
authors.lastname: Chernyshova
authors.forename: V V
authors.initials: VV
authors.lastname: Ignatov
mesh_terms: D000645:Ammonium Sulfate; D001937:Brassica / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D017077:Culture Media, Conditioned / Q000737:chemistry; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000494:pharmacology; D013696:Temperature; D016959:Xanthomonas campestris / Q000201:enzymology* / Q000254:growth & development
chemical_list: D017077:Culture Media, Conditioned; D009195:Peroxidase; D012697:Serine Endopeptidases; D000645:Ammonium Sulfate