Optimization of in situ hybridization protocols for detection of feline herpesvirus 1.
In situ hybridization (ISH) protocol including microwaving pre-treatment regimes was developed and compared with protease digestion as a pre-treatment regime for its effects on detecting feline herpesvirus 1 (FHV-1) in formalin fixed, paraffin embedded tissue. We found that optimum results were obtained using microwave pre-treatment. The results showed that the use of microwave irradiation would be recommended as a means of supplementing ISH methods, especially when using long-term formalin fixed, paraffin-embedded tissue.
Kim
Okjin
O
USDA-ARS, ADRU, USA.
Yi
Seong Joon
SJ
D000818:Animals; D002415:Cats / Q000821:virology*; D015342:DNA Probes; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary
D015342:DNA Probes