Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro- and methylaromatics: a new genomovar within the species.
Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a chromosomally encoded meta pathway. Phenotypic, chemotaxonomic and genotypic characterization classified strain CLN100 as a member of the species Pseudomonas stutzeri. DNA-DNA hybridizations, 16S rDNA, gyrB, rpoD sequences, and molecular fingerprinting indicate that strain CLN100 is a representative of a new genomovar (genomovar 10) within the species.
García-Valdés
Elena
E
Department de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanqats (CSIC-UIB), Palma de Mallorca, Spain. elena.garciavaldes@uib.es
Castillo
María M
MM
Bennasar
Antonio
A
Guasp
Caterina
C
Cladera
Aina M
AM
Bosch
Rafael
R
Engesser
Karl H
KH
Lalucat
Jorge
J
D001426:Bacterial Proteins / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D044223:Pseudomonas stutzeri / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012459:Salicylates / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009281:Naphthalenes; D020543:Proteome; D012336:RNA, Ribosomal, 16S; D012459:Salicylates; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase