title: Nuclear factor-kappaB activation in endothelium by Chlamydia pneumoniae without active infection.
abstract: Causative molecular mechanisms accounting for the potential link between Chlamydia pneumoniae and atherosclerosis are unknown. Formalin and heat-inactivated C. pneumoniae activated the transcription factor nuclear factor (NF)-kappaB in cultured porcine endothelium and up-regulated the expression of E-selectin messenger RNA and protein. This up-regulation was abolished by an IkappaB super-repressor, an NF-kappaB-specific inhibitor. Live bacteria are not necessary for the activation of endothelial NF-kappaB, and C. pneumoniae may contribute to atherogenesis without active infection.
authors.affiliation: Department of Pathology, University of North Carolina School of Medicine, North Carolina 27516, USA.
authors.forename: Jefferson T
authors.initials: JT
authors.lastname: Baer
authors.forename: Tracey V
authors.initials: TV
authors.lastname: Du Laney
authors.forename: Priscilla B
authors.initials: PB
authors.lastname: Wyrick
authors.forename: Arlene S
authors.initials: AS
authors.lastname: McCain
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Fischer
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Merricks
authors.forename: Albert S
authors.initials: AS
authors.lastname: Baldwin
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Nichols
mesh_terms: D000818:Animals; D001011:Aorta / Q000382:microbiology; D002478:Cells, Cultured; D016993:Chlamydophila pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D019040:E-Selectin / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D005557:Formaldehyde / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D013552:Swine; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D019040:E-Selectin; D016328:NF-kappa B; D005557:Formaldehyde