title: The bisphosphonate pamidronate is a potent inhibitor of Ewing's sarcoma cell growth in vitro.
abstract: The MTT assay was used to measure the effects of pamidronate, clodronate and mevastatin on the cell viability of Ewing's sarcoma cell lines 6647, CADO-ES-1, ES-2, ES-3, RD-ES, SK-ES-1, STA-ET-2.1 and VH-64. Treatment of these cells with pamidronate inhibited cell viability in a time- and dose-dependent manner. After a 72-h incubation period with 50 microM pamidronate, cell numbers were reduced by up to 80%, whereas the monophosphonate analog 3-aminopropyl phosphonate had no effect at concentrations up to 2 mM. Clodronate reduced cell viability by maximally 40% at 1 mM. These data provide the first evidence for a direct growth-inhibitory effect of pamidronate on Ewing's sarcoma cells. Hence, pamidronate definitely merits a more thorough exploration into its potential use in the therapy of patients with Ewing's sarcoma.
authors.affiliation: Labor für Experimentelle Orthopädie/Zellbiologie, Klinik und Poliklinik für Allgemeine Orthopädie, Universitätsklinikum Münster, Germany. Juergen.Sonnemann@uni-greifswald.de
authors.forename: Jürgen
authors.initials: J
authors.lastname: Sonnemann
authors.forename: Vera
authors.initials: V
authors.lastname: Eckervogt
authors.forename: Borna
authors.initials: B
authors.lastname: Truckenbrod
authors.forename: Joachim
authors.initials: J
authors.lastname: Boos
authors.forename: Winfried
authors.initials: W
authors.lastname: Winkelmann
authors.forename: Frans
authors.initials: F
authors.lastname: van Valen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004002:Clodronic Acid / Q000494:pharmacology; D004164:Diphosphonates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077268:Pamidronate; D012512:Sarcoma, Ewing
chemical_list: D000970:Antineoplastic Agents; D004164:Diphosphonates; D004002:Clodronic Acid; C012258:mevastatin; D008148:Lovastatin; D000077268:Pamidronate