Differential expression of c-fos in a mouse model of fetal alcohol syndrome.
OBJECTIVE
Fetal alcohol syndrome (FAS) results in stillbirth, fetal growth restriction, and mental retardation with injury attributed to oxidative stress. Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway.


METHODS
Timed, pregnant C57Bl6/J mice were injected on E8 with saline solution or alcohol. RNA was extracted from decidua and embryo 6 and 24 hours later. Microarray analysis was used to screen gene pathways. Differential gene expression was confirmed using real-time polymerase chain reaction with results presented as the ratio of c-fos concentration to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH).


RESULTS
Differential gene expression between alcohol and control was noted for stress signal pathway genes including c-fos. Real-time polymerase chain reaction demonstrated that c-fos messenger RNA expression was greater in the alcohol than control decidua at 6 hours after injection (P<.01). This effect persisted at 24 hours (P<.01). There was no difference in c-fos expression in embryos whose mothers received alcohol versus control after 6 hours (P=.12) or 24 hours (P=.89).


CONCLUSIONS
Alcohol administration during pregnancy results in differential gene expression in the stress signal pathway, particularly in c-fos. C-fos expression in the decidua increases from 6 to 24 hours after alcohol injection, but does not change in the embryo, which may contribute to alcohol-induced damage in FAS.
Poggi
Sarah H
SH
Department of Obstetrics and Gynecology, Georgetown University Hospital, Washington, DC, USA. shp2@gunet.georgetown.edu
Goodwin
Katie M
KM
Hill
Joanna M
JM
Brenneman
Douglas E
DE
Tendi
Elisabetta
E
Schninelli
Sergio
S
Spong
Catherine Y
CY
D000818:Animals; D003656:Decidua / Q000737:chemistry; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000737:chemistry; D000431:Ethanol / Q000008:administration & dosage; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000235:genetics*; D015870:Gene Expression; D016762:Genes, fos; D005865:Gestational Age; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D012333:RNA, Messenger; D000431:Ethanol; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases