title: Molecular characterization of squash leaf curl Yunnan virus, a new begomovirus and evidence for recombination.
abstract: Virus isolate Y23V, obtained from squash showing leaf curl symptoms in Yunnan, China, was readily differentiated from four studied Chinese begomovirus isolates in reactions with a set of monoclonal antibodies raised against begomoviruses. The complete nucleotide sequence (2714 nts) of the DNA-A-like molecule of Y23V was determined. The DNA-A of Y23V is most closely related to that of tomato yellow leaf curl Thailand virus-[1] (TYLCTHV-[1]) (84% sequence identity). However, the AC1 and AC4 gene of Y23V DNA-A resembled to Pepper leaf curl virus from Bangladesh (PepLCBDV). The DNA-A of Y23V has three distinct regions: the region from 74-2071 nts is 95% identical to TYLCTHV-[1] excluding a 27 nt deletion; the following 386 nts are 91% identical to PepLCBDV and the rest of the DNA-A is not closely related to any reported begomovirus. Y23V, therefore, is considered to have arisen by recombination. The 84% sequence identity of Y23V with TYLCTHV-[1] allows Y23V to be considered as a distinct begomovirus species, for which the name squash leaf curl Yunnan virus (SLCYNV) is proposed.
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: X P
authors.initials: XP
authors.lastname: Zhou
mesh_terms: D002681:China; D028464:Cucurbita / Q000821:virology; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA