The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology.
Acute phase proteins (APP) are synthesised in the liver in response to the systemic presence of high levels of pro-inflammatory cytokines. Bacteria are considered to be strong inducers of APP whereas viruses are weak or non-inducers of APP. Very few reports have been published on APP induction by parasites. Here, we report that the tick-borne protozoan parasite of cattle, Theileria annulata, induced an atypical acute phase response in cattle. Following experimental infection, serum amyloid A (SAA) appeared first, followed by a rise in alpha(1) acid glycoprotein (alpha(1)AGP) in all animals, whereas haptoglobin, which is a major APP in cattle, only appeared in some of the animals, and generally at a low level. All three APP only became elevated around or after the appearance of schizonts in draining lymph nodes and after the first observed temperature rise. Increased alpha(1)AGP levels coincided with the appearance of piroplasms. The production of SAA and alpha(1)AGP correlated strongly with each other, and also with some clinical measures of disease severity including the time to fever, development of leucopaenia, parasitaemia and mortality. These results are consistent with the hypothesis that T. annulata causes severe pathology in susceptible cattle by inducing high levels of pro-inflammatory cytokines.
Glass
Elizabeth J
EJ
Roslin Institute, Roslin, EH25 9PS, Midlothian, UK. liz.glass@bbsrc.ac.uk
Craigmile
Susan C
SC
Springbett
Anthea
A
Preston
Patricia M
PM
Kirvar
Erol
E
Wilkie
Gwen M
GM
Eckersall
P David
PD
Hall
F Roger
FR
Brown
C G Duncan
CG
D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D001053:Apolipoproteins / Q000032:analysis; D002417:Cattle; D006242:Haptoglobins / Q000032:analysis; D009961:Orosomucoid / Q000032:analysis; D000685:Serum Amyloid A Protein / Q000032:analysis; D016796:Theileria annulata; D013801:Theileriasis / Q000097:blood*
D000209:Acute-Phase Proteins; D001053:Apolipoproteins; D006242:Haptoglobins; D009961:Orosomucoid; D000685:Serum Amyloid A Protein