An integrated process for purification of lysozyme, ovalbumin, and ovomucoid from hen egg white.
This article describes an integrated process for simultaneous purification of lysozyme, ovalbumin, and ovomucoid from hen egg white. The crude egg white extract was passed through a cation exchanger Streamline trade mark SP and the bound lysozyme was eluted with 5% ammonium carbonate, pH 9.0, containing 1 M NaCl after elution of avidin. This partially purified lysozyme was further purified 639-fold on dye-linked cellulose beads. Ovalbumin and ovomucoid did not bind to Streamline SP. Ovalbumin could be precipitated from this unbound fraction by 5% trichloroacetic acid, and ovomucoid was removed from the supernatant by precipitation with ethanol. The yields of lysozyme, ovomucoid, and ovalbumin were 77, 94, and 98%, respectively. All the purified proteins showed single bands on sodium dodecyl sulfate polyacrylamide gel electrophoresis. All the steps are easily scalable, and the process described here can be used for large-scale simultaneous purification of these proteins in the pure form.
Roy
Ipsita
I
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Rao
M V S
MV
Gupta
Munishwar N
MN
D000465:Algorithms; D000818:Animals; D002482:Cellulose; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004529:Egg White / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000302:isolation & purification*; D010047:Ovalbumin / Q000302:isolation & purification*; D010059:Ovomucin / Q000302:isolation & purification*
D004396:Coloring Agents; D010059:Ovomucin; D002482:Cellulose; D010047:Ovalbumin; D009113:Muramidase