Triplex formation involving 2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) with 2-pyridone base analogue: efficient and selective recognition of C:G interruption.
For the effective recognition of C:G interruption in homopurine-homopyrimidine duplex DNA, we examined triplex-forming ability and sequence-selectivity of a triplex-forming oligonucleotide (TFO) involving of 2'-O,4'-C-methylene bridged nucleic acid with 2-pyridone base analogue. We found that the modified TFO formed stable triplex with high binding affinity and sequence-selectivity.
Torigoe
Hidetaka
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Shinjuku-ku, Tokyo, Japan. htorigoe@ch.kagu.tus.ac.jp
Hari
Yoshiyuki
Y
Obika
Satoshi
S
Imanishi
Takeshi
T
D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D003596:Cytosine; D006147:Guanine; D006838:Hydrocarbons; D008697:Methane / Q000031:analogs & derivatives*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013816:Thermodynamics
D006838:Hydrocarbons; D009838:Oligodeoxyribonucleotides; C030011:carbene; D006147:Guanine; D003596:Cytosine; D008697:Methane