Nucleotide sequence of the integration site of the temperate bacteriophage 6220, which carries the Shiga toxin gene stx(1ox3).
The integration site, attR, of the Shiga toxin-encoding phage 6220 (stx(1ox3)) has been determined. The phage integrates into the chromosome of its Escherichia coli host strain, CB6220, within a gene that is homologous to gene Z2577 and encodes an oxidoreductase. This new integration site was found in different Stx1ox3-producing enterohemorrhagic E. coli strains, which were analyzed by PCR.
Koch
Claudia
C
Schering AG, Biological Quality Control, D-13342 Berlin. Robert Koch-Institut, D-13353 Berlin, Germany.
Hertwig
Stefan
S
Appel
Bernd
B
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D016415:Sequence Alignment; D022621:Shiga Toxin / Q000235:genetics*; D022622:Shiga Toxin 1 / Q000235:genetics; D016662:Virus Integration / Q000235:genetics*
D022622:Shiga Toxin 1; D022621:Shiga Toxin; D010088:Oxidoreductases