Transition metal ligands as novel DNA-base substitutes.
The base modified nucleoside dBP, carrying a non-hydrogen-bonding non-shape complementary base was incorporated into oligonucleotides (Brotschi, C.; Häberli, A.; Leumann C.J. Angew. Chem. Int. Ed. 2001, 40, 3012-3014). This base was designed to coordinate transition metal ions into well defined positions within a DNA double helix. Melting experiments revealed that the stability of a dBP:dBP base couple in a DNA duplex is similar to a dG:dC base pair even in the absence of transition metal ions. In the presence of transition metal ions, melting experiments revealed a decrease in duplex stability which is on a similar order for all metal ions (Mn2+, Cu2+, Zn2+, Ni2+) tested.
Brotschi
Christine
C
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland. brotschi@ioc.unibe.ch
Leumann
Christian J
CJ
D020029:Base Pairing; D001483:Base Sequence; D002413:Cations, Divalent; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides; D008024:Ligands; D008670:Metals; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D002413:Cations, Divalent; D003854:Deoxyribonucleotides; D008024:Ligands; D008670:Metals; D009838:Oligodeoxyribonucleotides; D004247:DNA