Identification of quorum-sensing-regulated genes of Burkholderia cepacia.
Quorum sensing is a regulatory mechanism (operating in response to cell density) which in gram-negative bacteria usually involves the production of N-acyl homoserine lactones (HSL). Quorum sensing in Burkholderia cepacia has been associated with the regulation of expression of extracellular proteins and siderophores and also with the regulation of swarming and biofilm formation. In the present study, several quorum-sensing-controlled gene promoters of B. cepacia ATCC 25416 were identified and characterized. A total of 28 putative gene promoters show CepR-C(8)-HSL-dependent expression, suggesting that quorum sensing in B. cepacia is a global regulatory system.
Aguilar
Claudio
C
Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy. venturi@icgeb.org
Friscina
Arianna
A
Devescovi
Giulia
G
Kojic
Milan
M
Venturi
Vittorio
V
D001426:Bacterial Proteins / Q000378:metabolism*; D016956:Burkholderia cepacia / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007783:Lactones; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C117246:CepR protein, Burkholderia; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine