The effect of manganese chloride on gentamicin-induced nephrotoxicity in rats.
The aim of this study was to investigate the effects of manganese chloride on gentamicin-induced nephrotoxicity in rats. Thirty-six adult Wistar Albino rats were divided into six equal groups. They were injected with gentamicin sulfate (100 mg kg(-1) per day i.p.) and manganese chloride (2 or 20 mg kg(-1) per day i.p.) and gentamicin together with manganese chloride for 6 days. The animals were killed 24 h after the last injection. Nephrotoxicity was biochemically and histopathologically evaluated. The concentrations of creatinine, urea, sodium and potassium in plasma, malondialdehyde (MDA) and reduced glutathione (GSH) levels, glutathione peroxidase (GSH-Px) and catalase (CAT) activities in kidney tissue were determined. Administration of gentamicin to rats induced a marked renal failure, characterized with a significant increase in plasma creatinine and urea concentrations. A significant increase in kidney MDA and a decrease in GSH concentrations were observed in gentamicin-treated rats. No change was observed in the activities of GSH-Px and CAT in rats treated with gentamicin alone. Administration of the low dose of manganese (Mn2+) produced amelioration in biochemical indices of nephrotoxicity in plasma and kidney tissue when compared to gentamicin group. The histological signs of renal proximal tubules followed a similar pattern. The high dose of Mn2+ (20 mg kg(-1)) caused an opposite effect on nephrotoxicity induced by gentamicin, causing exacerbation in the tubular necrosis. The results suggest that low dose of Mn2+ may have an antioxidant effect in kidneys of gentamicin administrated rats, but its high doses had no beneficial effect.
Ateşşahin
A
A
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey. aatessahin@firat.edu.tr
Karahan
I
I
Yilmaz
S
S
Ceribaşi
A O
AO
Princci
I
I
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology* / Q000627:therapeutic use; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000008:administration & dosage* / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D008315:Malondialdehyde / Q000378:metabolism; D017895:Manganese Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D011188:Potassium / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000097:blood; D014508:Urea / Q000097:blood
D002712:Chlorides; D005839:Gentamicins; D017895:Manganese Compounds; D008315:Malondialdehyde; D014508:Urea; D012964:Sodium; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione; C025340:manganese chloride; D011188:Potassium