Targeted disruption of Streptomyces globisporus lndF and lndL cyclase genes involved in landomycin E biosynthesis.
Streptomyces globisporus strains with knockouts in lndF and lndL genes, previously identified as possibly encoding cyclases governing cyclization of the nascent oligoketide ('polyketide') chain during the biosynthesis of the antitumor angucycline landomycin E, were prepared. On combining the results of sequence analysis and HPLC of extracts from mutant strains, lndL was suggested to control the first cyclization-aromatization event and lndF to be responsible for the 3rd-4th ring formation.
Ostash
B
B
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, 790 05 L'viv, Ukraine.
Rebets
Y
Y
Yuskevich
V
V
Luzhetskyy
A
A
Tkachenko
V
V
Fedorenko
V
V
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000502:physiology*; D016254:Mutagenesis, Insertional; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*
D000617:Aminoglycosides; D001426:Bacterial Proteins; D004798:Enzymes; C113593:landomycin E