Increased expression of 19-kD interacting protein-3-like protein and the relationship to apoptosis in the lung of rats with severe acute pancreatitis.
OBJECTIVE
The aim of the present study was to determine the underlying cellular mechanisms in the pancreas after acute pancreatitis and to study the pathogenesis of pancreatitis-associated lung injury. We applied a differential display analysis to normal pancreas and to the pancreas with acute pancreatitis in rats, and we examined the expression of the identified gene in the lung as well as the pancreas after acute pancreatitis.


METHODS
Controlled animal study.


METHODS
Research laboratory of an academic institution.


METHODS
Ninety male Wistar rats.


METHODS
Pancreatitis was induced by retrograde intraductal infusion of 4% sodium taurocholate (100 microL/100 g of body weight). Data were compared with data from controls (sham).


RESULTS
We cloned some expressed sequence tags and identified one complementary DNA fragment. The deduced protein was a polypeptide of 218 amino acids, which was almost identical to human 19-kD interacting protein-3-like (NIP3L) protein. The expression of rat NIP3L identified in this study increased slightly in the pancreas after induction of acute pancreatitis but showed a marked increase in the lung by both Northern and Western blot analysis. NIP3L immunoreactivity was noted in alveolar and epithelial cells of the control (sham) lung, and the immunoreactivity in these cells was elevated after induction of acute pancreatitis. Moreover, acute pancreatitis increased terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling-positive alveolar and bronchiolar cells in the lung.


CONCLUSIONS
NIP3L may be involved in lung injury, which is one of the major causes of death in cases of severe acute pancreatitis.
Nakamura
Hayato
H
Third Department of Internal Medicine, University of Occupational and Environmental Health, Japan, School of Medicine, Yahatanashi, Kitakyushu, Japan.
Honda
Hidekazu
H
Tashiro
Mitsuo
M
Taguchi
Masashi
M
Yoshikawa
Hiroyuki
H
Otsuki
Makoto
M
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D020287:In Situ Nick-End Labeling; D008171:Lung Diseases / Q000209:etiology*; D008297:Male; D010195:Pancreatitis / Q000150:complications*; D011506:Proteins / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000209:etiology* / Q000473:pathology
D011506:Proteins