title: Characterization of infectious bursal disease virus isolates from Indonesia indicates the existence of very virulent strains with unique genetic changes.
abstract: Sequencing of the hypervariable region of viral protein VP2 of infectious bursal disease virus (IBDV) isolates obtained from non-vaccinated chickens in Indonesia showed that the majority (16/17) were closely related to published very virulent (vv)IBDV strains. Four isolates contained identical amino acid sequences to Asian and European vvIBDVs, sharing vv-specific amino acid residues 222(Ala), 256(Ile), and 294(Ile). Eight isolates differed by one amino acid at position 222(Ala-->Ser); however, this change did not alter the pathogenicity or antigenicity of these strains. Two isolates, with amino acid substitutions at positions 272(Ile-->Thr) and 279(Asp-->Asn), did not cause clinical disease or mortality, and were therefore considered to be naturally occurring, attenuated mutants of vvIBDV. The results illustrate variability that might occur among vvIBDV strains.
authors.affiliation: Balai Penelitian Veteriner, Jl. RE Martadinata 30, Bogor 16114, Indonesia.
authors.forename: L H
authors.initials: LH
authors.lastname: Parede
authors.forename: S
authors.initials: S
authors.lastname: Sapats
authors.forename: G
authors.initials: G
authors.lastname: Gould
authors.forename: M
authors.initials: M
authors.lastname: Rudd
authors.forename: S
authors.initials: S
authors.lastname: Lowther
authors.forename: J
authors.initials: J
authors.lastname: Ignjatovic
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry; D018175:Birnaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D014644:Genetic Variation; D007214:Indonesia; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000401:mortality / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral