Conversion of cholesterol injected into man to cholestanol via a 3-ketonic intermediate.
Cholesterol-3-(3)H,4-(14)C was injected intravenously in man and its transformation to cholestanol was studied. From the (3)H: (14)C ratios in cholestanol isolated from blood, evidence for the participation of a ketonic intermediate in the conversion was obtained. In a second subject given cholestanol-3-(3)H,4-(14)C the (3)H: (14)C ratios in blood sterols remained unchanged for as long as 1 wk after the injection, which showed that cholestanol did not lose tritium by interconversion with cholestanone.
Rosenfeld
R S
RS
Institute for Steroid Research and the Division of Neoplastic Medicine, Montefiore Hospital and Medical Center, New York, USA.
Zumoff
B
B
Hellman
L
L
D000328:Adult; D002250:Carbon Radioisotopes; D004083:Cholestanol / Q000097:blood / Q000378:metabolism*; D002784:Cholesterol / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D007659:Ketones / Q000097:blood / Q000378:metabolism; D008297:Male; D014316:Tritium
D002250:Carbon Radioisotopes; D007659:Ketones; D014316:Tritium; D004083:Cholestanol; D002784:Cholesterol