AtAGP30, an arabinogalactan-protein in the cell walls of the primary root, plays a role in root regeneration and seed germination.
Arabinogalactan-proteins (AGPs) are extracellular proteoglycans that are implicated in many plant growth and developmental processes, but in no case has a biological function been assigned to a particular AGP. AtAGP30 is a non-classical AGP core protein from Arabidopsis that is expressed only in roots. Analysis of the corresponding mutant, agp30, has revealed that the wild-type gene product is required in vitro for root regeneration and in planta for the timing of seed germination. The mutant shows a suppression of the abscisic acid (ABA)-induced delay in germination and altered expression of some ABA-regulated genes. This suggests that AtAGP30 functions in the ABA response. By analogy to proteoglycan-mediated regulation of growth-factor-signalling pathways in animals, our data indicate that phytohormone activity in plants can be modulated by AGPs.
van Hengel
Arjon J
AJ
Department of Cell and Developmental Biology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK. keith.roberts@bbsrc.ac.uk
Roberts
Keith
K
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination / Q000502:physiology*; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins; D018517:Plant Roots / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000502:physiology*
D029681:Arabidopsis Proteins; D002241:Carbohydrates; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins