Identification and characterization of a Zea mays line carrying a transposon-tagged ENOD40.
In Zea mays, two ENOD40 homologous were identified that show only 30% of sequence homology to each other. We identified line e40-mum1 carrying a Mu transposon inserted in ZmENOD40-1, the maize gene that has the highest homology to leguminous ENOD40. The insertion causes a dramatic reduction of the ZmENOD40-1 transcript level. Irrespective of this, homozygous e40-mum1 plants are still able to interact with mycorrhizal fungi. Furthermore, no phenotypic aberrations correlated to the presence of e40-mum1 have been identified and therefore it is suggested that Z. mays ENOD40 genes are functionally redundant despite their strikingly low homology.
Compaan
Bert
B
Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Ruttink
Tom
T
Albrecht
Cathy
C
Meeley
Robert
R
Bisseling
Ton
T
Franssen
Henk
H
D001483:Base Sequence; D004251:DNA Transposable Elements; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D038821:Mycorrhizae; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D010940:Plant Proteins