Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization.
OBJECTIVE
To quantify aneuploidy in inseminated, injected, and noninjected oocytes from infertility patients using Multifluor fluorescence in situ hybridization (M-FISH).


METHODS
Prospective study.


METHODS
Reproductive biology group, academic unit of pediatrics, obstetrics, and gynecology.


METHODS
Forty-eight patients undergoing ovarian stimulation and either intracytoplasmic sperm injection (ICSI) or conventional in vitro fertilization (IVF).


METHODS
None.


METHODS
M-FISH karyotyping of 67 metaphase II oocytes, including noninjected in vitro matured oocytes, and injected inseminated-failed fertilized oocytes.


RESULTS
Thirty-nine percent of oocytes were aneuploid, with nondisjunction of chromosomes in 34% of oocytes and predivision of chromatids in 10%. There was no difference in aneuploidy rates between ICSI noninjected in vitro matured oocytes and injected, failed fertilized oocytes. Chromosomes most frequently involved in aneuploidy were 15, 18, 19, 22, and X. In seven injected ICSI MII oocytes, the prematurely condensed sperm chromatin was karyotyped by M-FISH.


CONCLUSIONS
M-FISH was used to diagnose aneuploidy at maternal meiosis I in 39% of oocytes, and M-FISH karyotyping of sperm was demonstrated.
Clyde
Julie M
JM
Reproductive Biology Group, Academic Unit of Paediatrics, Obstetrics, and Gynaecology, University of Leeds, Leeds, United Kingdom.
Hogg
Jan E
JE
Rutherford
Anthony J
AJ
Picton
Helen M
HM
D000328:Adult; D000782:Aneuploidy; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007247:Infertility, Female / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D007621:Karyotyping; D008677:Metaphase; D009865:Oocytes; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016707:Tissue Fixation