The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160.
In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direct coupling to an RNA binding protein Scp160. These findings suggest an additional role for Gpa1 and reveal Scp160 as a component of the mating response pathway in yeast.
Guo
Ming
M
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Aston
Christopher
C
Burchett
Scott A
SA
Dyke
Christine
C
Fields
Stanley
S
Rajarao
S Johannes R
SJ
Uetz
Peter
P
Wang
Yuqi
Y
Young
Kathleen
K
Dohlman
Henrik G
HG
D004305:Dose-Response Relationship, Drug; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism* / Q000502:physiology*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D005690:Galactose / Q000378:metabolism; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
D044385:GTP-Binding Protein alpha Subunits; D008565:Membrane Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C097262:SCP160 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D005982:Glutathione Transferase; C084293:GPA1 protein, S cerevisiae; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D005947:Glucose; D005690:Galactose