Comparison of usefulness of randomly amplified polymorphic DNA and amplified-fragment length polymorphism techniques in epidemiological studies on nasopharyngeal carriage of non-typable Haemophilus influenzae.
Randomly amplified polymorphic DNA (RAPD) and automated amplified-fragment length polymorphism (AFLP) techniques with fluorescently labelled primers were used to type non-serotypable Haemophilus influenzae (NTHI) isolates. Eighty-seven isolates from healthy children attending day-care centres or living at orphanages in southern Poland were investigated. Through comparison of the AFLP data with RAPD analysis, it has been concluded that the discriminatory power of AFLP for NTHI typing is higher than RAPD. Generally, the NTHI isolates analysed were highly heterogeneous, as detected with a HindIII/TaqI AFLP genotyping scheme on intra/inter similarity levels of 94 and 96 % using Pearson's correlation coefficient. The range of similarity values found for isolates from children permanently residing at a particular day-care centre was much wider than that for isolates from orphanages. AFLP can efficiently access NTHI strain diversity and can monitor their turn-over for comparative typing in local and inter-local epidemiological investigations.
Augustynowicz
Ewa
E
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
Gzyl
Anna
A
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
Szenborn
Leszek
L
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
Banys
Dorota
D
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
Gniadek
Grzegorz
G
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
Ślusarczyk
Janusz
J
Department of Sera and Vaccine Evaluation, National Institute of Hygiene, Chocimska 24 St, 00-791 Warsaw, Poland 2Department of Infectious Diseases of Children, Medical University, Wroclaw, Poland.
D015373:Bacterial Typing Techniques / Q000379:methods*; D002353:Carrier State / Q000382:microbiology*; D002675:Child, Preschool; D016021:Epidemiologic Studies; D006193:Haemophilus influenzae / Q000145:classification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*