title: Talin binding to integrin beta tails: a final common step in integrin activation.
abstract: Control of integrin affinity for ligands (integrin activation) is essential for normal cell adhesion, migration, and assembly of an extracellular matrix. Integrin activation is usually mediated through the integrin beta subunit cytoplasmic tail and can be regulated by many different biochemical signaling pathways. We report that specific binding of the cytoskeletal protein talin to integrin beta subunit cytoplasmic tails leads to the conformational rearrangements of integrin extracellular domains that increase their affinity. Thus, regulated binding of talin to integrin beta tails is a final common element of cellular signaling cascades that control integrin activation.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, The Burnham Institute, La Jolla, CA 92037, USA.
authors.forename: Seiji
authors.initials: S
authors.lastname: Tadokoro
authors.forename: Sanford J
authors.initials: SJ
authors.lastname: Shattil
authors.forename: Koji
authors.initials: K
authors.lastname: Eto
authors.forename: Vera
authors.initials: V
authors.lastname: Tai
authors.forename: Robert C
authors.initials: RC
authors.lastname: Liddington
authors.forename: Jose M
authors.initials: JM
authors.lastname: de Pereda
authors.forename: Mark H
authors.initials: MH
authors.lastname: Ginsberg
authors.forename: David A
authors.initials: DA
authors.lastname: Calderwood
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D039641:Integrin beta Chains / Q000737:chemistry / Q000378:metabolism*; D019012:Integrin beta1 / Q000737:chemistry / Q000378:metabolism; D039661:Integrin beta3 / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D016608:Talin / Q000378:metabolism*; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D039641:Integrin beta Chains; D019012:Integrin beta1; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D016608:Talin