Studies on the safety and immunogenicity of the South African bluetongue virus serotype 2 monovalent vaccine: specific detection of the vaccine strain genome by RT-PCR.
In order to study the safety and the immunogenicity of the South African vaccine against the serotype 2 bluetongue virus, two groups of seven sheep were vaccinated with the vaccine used in the French island of Corsica. Vaccinated and non-vaccinated sheep were observed clinically and their rectal temperatures were recorded daily. The serological response in vaccinated animals confirmed the immunogenicity of the vaccine. Post-vaccinal viraemia was investigated and the vaccine genome was detected by reverse transcription polymerase chain reaction (RT-PCR). No viraemia was observed at post-vaccination days 4, 7 and 11 but the vaccine strain of virus was detected by RT-PCR throughout the experiment. The thermostability of the vaccine was also evaluated. The vaccine titre strongly decreased at temperatures higher than 35 degrees C.
Hammoumi
S
S
Agence Française de Sécurité Sanitaire des Aliments, Maisons-Alfort, France.
Breard
E
E
Sailleau
C
C
Russo
P
P
Grillet
C
C
Cetre-Sossah
C
C
Albina
E
E
Sanchis
R
R
Pepin
M
M
Guibert
J-M
JM
Zientara
S
S
D000818:Animals; D001819:Bluetongue / Q000097:blood / Q000517:prevention & control*; D001820:Bluetongue virus / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D001831:Body Temperature; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016679:Genome, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping; D012756:Sheep; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines; D014766:Viremia / Q000662:veterinary
D014765:Viral Vaccines