title: Myocardial cooling for reperfusion injury protection achieved by organ specific hypothermic autologous perfusion.
abstract: OBJECTIVE
  Whole body hypothermia has been suggested to reduce myocardial injury in patients with ST-segment elevation myocardial infarction. Because of the large human thermal mass, induction of generalized hypothermia is slow and the technique has encountered considerable side effects. The aim was to develop and validate a method for regional cooling during myocardial reperfusion using hypothermic autologous blood.
  
  
  METHODS
  In a myocardial ischemia-reperfusion pig model (n = 10), arterial blood was cooled in a closed circuit, and returned to the myocardium during reperfusion either through a perfusion catheter or through the guiding catheter. Myocardial temperatures were recorded using temperature electrodes.
  
  
  RESULTS
  Stabile regional myocardial cooling was induced without complications within 4 min. Both flow rate and blood temperature had significant impact on temperature in the reperfused myocardium but did not influence systemic temperature.
  
  
  CONCLUSIONS
  A method for organ specific hypothermic autologous arterial blood reperfusion has been developed and validated. The method is a simple and much faster alternative to systemic cooling and may have the potential to reduce myocardial injury in patients with acute myocardial infarction.
authors.affiliation: Department of Cardiology B, Skejby Sygehus, Aarhus University Hospital, Institute of Experimental Clinical Research, Aarhus University, Denmark. michael.maeng@iekf.au.dk
authors.forename: Michael
authors.initials: M
authors.lastname: Maeng
authors.forename: Steen Buus
authors.initials: SB
authors.lastname: Kristiansen
authors.forename: Ulrik Markus
authors.initials: UM
authors.lastname: Mortensen
authors.forename: Henning Rud
authors.initials: HR
authors.lastname: Andersen
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001804:Blood Transfusion, Autologous; D001831:Body Temperature; D006328:Cardiac Catheterization; D006339:Heart Rate; D007036:Hypothermia, Induced / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium; D013552:Swine