title: Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC.
abstract: Prolonged activation of metabotropic glutamate receptor 5a (mGluR5a) causes synchronized oscillations in intracellular calcium, inositol 1,4,5-trisphosphate production, and protein kinase C (PKC) activation. Additionally, mGluR5 stimulation elicited cyclical translocations of myristoylated alanine-rich protein kinase C substrate, which were opposite to that of gammaPKC (i.e. from plasma membrane to cytosol) and dependent on PKC activity, indicating that myristoylated alanine-rich protein kinase C substrate is repetitively phosphorylated by oscillating gammaPKC on the plasma membrane. Mutation of mGluR5 Thr(840) to aspartate abolished the oscillation of gammaPKC, but the mutation to alanine (T840A) did not. Cotransfection of gammaPKC with betaIIPKC, another Ca2+-dependent PKC, resulted in synchronous oscillatory translocation of both classical PKCs. In contrast, cotransfection of deltaPKC, a Ca2+-independent PKC, abolished the oscillations of both gammaPKC and inositol 1,4,5-trisphosphate. Regulation of the oscillations was dependent on deltaPKC kinase activity but not on gammaPKC. Furthermore, the T840A-mGluR5-mediated oscillations were not blocked by the deltaPKC overexpression. These results revealed that activation of mGluR5 causes translocation of both gammaPKC and deltaPKC to the plasma membrane. deltaPKC, but not gammaPKC, phosphorylates mGluR5 Thr(840), leading to the blockade of both Ca2+ oscillations and gammaPKC cycling. This subtype-specific targeting proposes the molecular basis of the multiple functions of PKC.
authors.affiliation: Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Uchino
authors.forename: Norio
authors.initials: N
authors.lastname: Sakai
authors.forename: Kaori
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shirai
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hirose
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Iino
authors.forename: Takehira
authors.initials: T
authors.lastname: Yamamura
authors.forename: Naoaki
authors.initials: N
authors.lastname: Saito
mesh_terms: D020013:Calcium Signaling; D002478:Cells, Cultured; D004789:Enzyme Activation; D006801:Humans; D010766:Phosphorylation; D011493:Protein Kinase C / Q000502:physiology*; D051745:Protein Kinase C-delta; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000494:pharmacology; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000502:physiology*
chemical_list: C578935:GRM5 protein, human; D034741:RNA, Small Interfering; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; C087857:protein kinase C gamma; C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta