title: Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro- and methylaromatics: a new genomovar within the species.
abstract: Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a chromosomally encoded meta pathway. Phenotypic, chemotaxonomic and genotypic characterization classified strain CLN100 as a member of the species Pseudomonas stutzeri. DNA-DNA hybridizations, 16S rDNA, gyrB, rpoD sequences, and molecular fingerprinting indicate that strain CLN100 is a representative of a new genomovar (genomovar 10) within the species.
authors.affiliation: Department de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanqats (CSIC-UIB), Palma de Mallorca, Spain. elena.garciavaldes@uib.es
authors.forename: Elena
authors.initials: E
authors.lastname: García-Valdés
authors.forename: María M
authors.initials: MM
authors.lastname: Castillo
authors.forename: Antonio
authors.initials: A
authors.lastname: Bennasar
authors.forename: Caterina
authors.initials: C
authors.lastname: Guasp
authors.forename: Aina M
authors.initials: AM
authors.lastname: Cladera
authors.forename: Rafael
authors.initials: R
authors.lastname: Bosch
authors.forename: Karl H
authors.initials: KH
authors.lastname: Engesser
authors.forename: Jorge
authors.initials: J
authors.lastname: Lalucat
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D044223:Pseudomonas stutzeri / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012459:Salicylates / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009281:Naphthalenes; D020543:Proteome; D012336:RNA, Ribosomal, 16S; D012459:Salicylates; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase