MALDI-TOF mass spectrometry as a powerful tool to study enzymatic processing of DNA lesions inserted into oligonucleotides.
MALDI-TOF mass spectrometry measurements, coupled with either exonuclease or DNA N-glycosylases digestions of lesion-containing oligonucleotides, were used to assess biochemical features of several oxidative DNA damage. The latter analytical approach was shown to be an informative and efficient alternative technique to conventional electrophoresis and chromatographic analyses.
Gasparutto
D
D
Laboratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique-UMR 5046, DRFMC, CEA-Grenoble, Grenoble Cedex, France. dgasparutto@drfmc.ceng.cea.fr
Saint-Pierre
C
C
Jaquinod
M
M
Favier
A
A
Cadet
J
J
D000818:Animals; D020137:Base Pair Mismatch / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D045647:DNA Glycosylases; D003851:Deoxyribonucleases; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D043264:Phosphodiesterase I; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009838:Oligodeoxyribonucleotides; D004247:DNA; D003851:Deoxyribonucleases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom; D045647:DNA Glycosylases