title: Role of apoptosis and mitosis during human eye development.
abstract: The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic cells were found in the neuroepithelium of the optic cup and stalk, being the most numerous at the borderline between the two layers of the optic cup, and at the place of transition of the optic cup into stalk. They were also found at the region of detachment of the lens pit from the surface ectoderm. In the later developmental stages (the 6th-the 9th week), apoptotic and mitotic cells were observed in the neural retina and the anterior lens epithelium. Throughout all stages examined, mitotic cells were found exclusively adjacent to the lumen either of the intraretinal space or the optic stalk ventricle, or were restricted to the superficial epithelial layer of the lens primordium. Unlike mitotic cells, apoptotic cells occurred throughout the whole width both of the neuroepithelium and the surface epithelium. Ultrastructurally, apoptotic cells were characterised by round- or crescent-shaped condensations of chromatin near the nuclear membrane, while in the more advanced stages of apoptosis by apoptotic bodies. The distribution of caspase-3-positive cells coincided with the location of apoptotic cells described by morphological techniques indicating that the caspase-3-dependent apoptotic pathway operates during the all stages of human eye development. The location of cells positive for anti-apoptotic bcl-2 protein was in accordance with the regions of eye with high mitotic activity, confirming the role of bcl-2 in protecting cells from apoptosis. In the earliest stage of eye development, apoptosis and mitosis might be associated with the sculpturing of the walls of optic cup and stalk, while high mitotic activity along the intraretinal space and optic stalk ventricle indicates its role in the gradual luminal closure. These processes also participate in the detachment of the lens pit epithelium from the surface ectoderm as well as in further closure of the lens vesicle. Later on, both processes seem to be involved in the neural retina differentiation, lens morphogenesis and secondary lens fibre differentiation.
authors.affiliation: Department of Anatomy, Histology and Embryology, Medical School, University of Split, Split, Croatia. db@mefst.hr
authors.forename: Darka
authors.initials: D
authors.lastname: Bozanić
authors.forename: Robert
authors.initials: R
authors.lastname: Tafra
authors.forename: Mirna
authors.initials: M
authors.lastname: Saraga-Babić
mesh_terms: D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D005123:Eye / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D005865:Gestational Age; D006801:Humans; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology*; D009024:Morphogenesis; D012160:Retina / Q000196:embryology / Q000378:metabolism
chemical_list: C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases