Conditional expression of MCM7 increases tumor growth without altering DNA replication activity.
The minichromosome maintenance (MCM) 2-7 complex is a putative DNA helicase complex that facilitates the initiation of DNA replication. Here, we generated a cell line MCM7(+/-)/MCM7-FLAG, in which one allele of MCM7 is mutated whereas a tetracycline-repressible promoter could manipulate the expression of exogenous MCM7 protein. Overexpressed MCM7 protein supports efficient DNA replication of Epstein-Barr virus oriP and rapid formation of tumors in nude mice without altering the activity of cellular DNA replication. This system provides a unique setting for studying the function of MCM7 and for screening for potential therapeutics for malignant tumors.
Yoshida
Kenichi
K
Genetic Diagnosis, Institute of Medical Science, University of Tokyo, Japan. yoshidak@ims.u-tokyo.ac.jp
Inoue
Ituro
I
D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D064168:Minichromosome Maintenance Complex Component 7; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D018741:Replication Origin / Q000235:genetics; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C101624:MCM7 protein, human; C491086:Mcm7 protein, mouse; D064168:Minichromosome Maintenance Complex Component 7