[ATP-generating creatine kinase: a new compound isolated from Viperidae venom].
This is a first report on the presence of creatine kinase, ATP-generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin-thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
Kuznetsov
D A
DA
Semenov Institute of Chemical Physics, RAS, Moscow, Russia.
Orlova
M A
MA
Berdieva
A G
AG
Khasigov
P Z
PZ
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D002384:Catalysis; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D014757:Viper Venoms / Q000201:enzymology*
D014757:Viper Venoms; D000255:Adenosine Triphosphate; D003402:Creatine Kinase