Isolation, characterization and expression of a cyclin from Leishmania donovani.
We have cloned and sequenced a DNA fragment (approximately 1 kb) containing a complete open reading frame from a cDNA library of Leishmania donovani promastigotes. The alignment of the derived polypeptide sequence and the modeling studies revealed that the protein is highly homologous to the mammalian cyclins having conserved cyclin box and substrate-docking motif. Northern blot analysis of the RNA isolated from synchronized L. donovani promastigotes showed periodic expression of the message with maximum abundance at S-phase suggesting its involvement in the events related to the regulation of DNA replication. The results confirm that we have isolated a cyclin molecule from L. donovani (LdCyc1) which may play an important role in the regulation of the parasite cell cycle.
Banerjee
Sampali
S
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, I/AF Bidhannagar, Kolkata 700064, India.
Banerjee
Rahul
R
Das
Ruma
R
Duttagupta
Swadesh
S
Saha
Partha
P
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016213:Cyclins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D004261:DNA Replication / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D007893:Leishmania donovani / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D016213:Cyclins; D012333:RNA, Messenger