title: Dicer is essential for mouse development.
abstract: To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.
authors.affiliation: Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Bernstein
authors.forename: Sang Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Carmell
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Murchison
authors.forename: Heather
authors.initials: H
authors.lastname: Alcorn
authors.forename: Mamie Z
authors.initials: MZ
authors.lastname: Li
authors.forename: Alea A
authors.initials: AA
authors.lastname: Mills
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elledge
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Anderson
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Hannon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053487:DEAD-box RNA Helicases; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004722:Endoribonucleases / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D043244:Ribonuclease III; D013234:Stem Cells / Q000166:cytology
chemical_list: D004722:Endoribonucleases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases