title: Amplified-fragment length polymorphism as a complement to IS6110-based restriction fragment length polymorphism analysis for molecular typing of Mycobacterium tuberculosis.
abstract: The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.
authors.affiliation: Section of Microbiology, Hospital General Universitario de Elche, Elche (Alicante), Spain. micro_elx@gva.es
authors.forename: Monserrat
authors.initials: M
authors.lastname: Ruiz
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Rodríguez
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Valera
authors.forename: Gloria
authors.initials: G
authors.lastname: Royo
mesh_terms: D015373:Bacterial Typing Techniques; D004251:DNA Transposable Elements; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements