Phospholipase A2 activity in three species of littoral freshwater rotifers exposed to several toxicants.
We analyzed three species of Lecane, a littoral rotifer, for susceptibility to six metals and four organic toxicants using a fluorometric assay based on inhibition of activity of the enzyme phospholipase A2. The metallic toxicants that we tested included Cd, Cr, Cu, Pb, Hg (as HgCl2), and Ti; the organic toxicants included benzene, ethyl acetate, toluene, and vinyl acetate. The three species differed greatly with respect to their susceptibility to the various toxicants. Lecane quadridentata, for example, was particularly sensitive to the four organic compounds (median effective concentration values [EC50] ranged from 6.6 x 10(-4)-0.987 mg/L). Lecane luna, in contrast, seemed particularly sensitive to metals (EC50 values ranged from 2 x 10(-6)-1.92 mg/L). Lecane hamata was relatively insensitive to organic solvents (EC50 values ranged from 4.25-126.5 mg/L).
Pérez-Legaspi
Ignacio Alejandro
IA
Universidad Autónoma de Aguascalientes, Centro de Ciencias Básicas, Departamento de Química, Avenida Universidad 940, C.P. 20100, Aguascalientes, Ags., México.
Rico-Martínez
Roberto
R
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004781:Environmental Exposure; D019216:Metals, Heavy / Q000633:toxicity*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D012403:Rotifera / Q000201:enzymology*; D012997:Solvents / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D019216:Metals, Heavy; D012997:Solvents; D014874:Water Pollutants, Chemical; D010741:Phospholipases A; D054467:Phospholipases A2