[Effect of endotoxemia on thyrotropin releasing hormone and its receptor in Wistar rat brain].
OBJECTIVE
To study the dose and location of thyrotropin releasing hormone (TRH) and its receptor in Wistar rat's brain when early endotoxemia happened.


METHODS
Twenty-two Wistar rats were selected. In 13 mouse the model was established with endotoxemia and hemorrhagic shock. The TRH and its receptor were observed with radioimmunoassay (RIA), and were compared to the 9 control rats.


RESULTS
The dose of TRH decreased significantly in the early stage of endotoxemia. The maxium dose (Bmax) of TRH increased while Kd (affinity) decreased, so the effective TRH receptor had no significantly change.


CONCLUSIONS
The dose of TRH is different in brain and serum. The decrease of TRH and the steady of active TRH receptor may protect the brain tissue and repress multiple organ dysfunction syndrome (MODS) in the early stage of endotoxemia.
Wang
Xin-chun
XC
Emergency Department, Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang, China.
Liu
Bei-yan
BY
Xu
Ping
P
Jing
Hui
H
D000818:Animals; D001923:Brain Chemistry; D019446:Endotoxemia / Q000378:metabolism*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000032:analysis*; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis*
D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone