[Effect of echo-contrast agent on rat vascular smooth muscle cell proliferation during ultrasound exposure].
OBJECTIVE
To investigate the effect of echo-contrast agent on rat vascular smooth muscle cell (VSMC) proliferation in the presence of ultrasound exposure.


METHODS
The VSMCs of rats were cultured in 6-well plates with or without the echo-contrast agent and stained with trypan blue at 3, 24, and 48 h respectively after ultrasound exposure for 1 min at 2 MHz, 0.25 mechanical index (MI). At each time point as indicated after the exposure, the cells in parallel wells were trypsinized for cell counting using Coulter counter.


RESULTS
No cytotoxicity was found in trypan blue staining. Compared with the control cells, the proliferation of VSMCs was inhibited by echo-contrast agent and adjunctive ultrasound within 24 h, but another 24 h later, the effect failed to be observed.


CONCLUSIONS
The proliferation of VSMC was inhibited by with low mechanical index cavitation activity of contrast agents.
Xiu
Jian-cheng
JC
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. xiujc@fimmu.com
Ou
Wen-chao
WC
Liu
Jian
J
Lai
Wen-yan
WY
Liu
Yi-li
YL
D015906:Angioplasty, Balloon, Coronary; D000818:Animals; D002478:Cells, Cultured; D003287:Contrast Media / Q000633:toxicity*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014465:Ultrasonics
D003287:Contrast Media