[Study on anti-tumor activity of extracts from cultured cells of Taxus chinensis].
OBJECTIVE
To analyze the cytotoxicity and inhibitory effect of extracts from cultured cells(from stems) of Taxus chinensis on cancer cell line SMMC-7721.


METHODS
MTT assay for cell viability and flow cytometry for cell cycle analysis.


RESULTS
MTT results showed that the extact by dichloromethane was more effective than that by methanol on SMMC-7721, IC50 were 0.0834 mg DCW.ml-1 and 0.8002 mg DCW.ml-1 respectively. SMMC-7721 cells in G2-M stage increased with higher concentration of dichloromethane extracts from Taxus chinensis cells and longer incubation.


CONCLUSIONS
Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC-7721 and could induce apoptosis of cancer cells.
Lu
Cuitao
C
School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074.
Mei
Xingguo
X
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002453:Cell Cycle; D002478:Cells, Cultured / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020946:Taxus / Q000737:chemistry / Q000166:cytology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal