Metabolic polymorphisms and the micronucleus frequency in buccal epithelium of adolescents living in an urban environment.
Micronuclei and other biomarkers were evaluated in oral cells from 11- to 16-year-old girls living in a foster home in the central area of México City. Variables analyzed for possible association with these biomarkers include smoking habits, body mass index, metabolic polymorphisms for NAT1 and GSTM1 and whether the cells were obtained from the cheek or pharynx. The results indicated that individuals having the NAT1*10 homozygous genotype showed a significant increase in chromatin buds and binucleated cells. When the damage in the cheek was compared with damage in the pharynx, a significant increase in micronuclei and binucleated cells was found for the latter tissue in all the individuals analyzed.
Montero
Regina
R
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), México, D.F. dorinda@servidor.unam.mx
Serrano
Luis
L
Dávila
Víctor
V
Segura
Yayoi
Y
Arrieta
Andrea
A
Fuentes
Raquel
R
Abad
Iris
I
Valencia
Lucía
L
Sierra
Patricia
P
Camacho
Rafael
R
D000293:Adolescent; D001191:Arylamine N-Acetyltransferase / Q000235:genetics; D001483:Base Sequence; D015415:Biomarkers; D015992:Body Mass Index; D001835:Body Weight; D017931:DNA Primers; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000378:metabolism / Q000648:ultrastructure*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011795:Surveys and Questionnaires; D014505:Urban Population
D015415:Biomarkers; D017931:DNA Primers; D007527:Isoenzymes; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1; D005982:Glutathione Transferase