title: Synthesis of DNA conjugates by solid phase fragment condensation.
abstract: Development of a novel method for the synthesis of DNA conjugates is described. Oligonucleotides were successfully conjugated with a variety of functional molecules on a solid phase (Solid Phase Fragment Condensation) using an amino, a hydroxyl, a thiol, and a carboxyl group. DNA-peptide conjugate was obtained as a pure from by a single RPHPLC purification approximately in 20% yield. Moreover, it was demonstrated that the present method was effective for the preparation of conjugate molecules, DNA-sugar, DNA-polyamine, DNA-lipid and so on. The study to create new intelligent DNAs by accumulation various biofunctions on the molecule by SPFC is now in progress in our laboratory.
authors.affiliation: Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, Iizuka, Fukuoka, Japan. yanno@iron.chem.fuk.kindai.ac.jp
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Anno
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Ryoji
authors.initials: R
authors.lastname: Ueki
authors.forename: Mayuka
authors.initials: M
authors.lastname: Yano
authors.forename: Kotomi
authors.initials: K
authors.lastname: Sasaki
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D004247:DNA