Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow.
Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin alpha4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation.
De Ugarte
Daniel A
DA
Department of Surgery, UCLA School of Medicine, Regenerative Bioengineering and Repair Laboratory, Los Angeles, CA, USA. deugarte@ucla.edu
Alfonso
Zeni
Z
Zuk
Patricia A
PA
Elbarbary
Amir
A
Zhu
Min
M
Ashjian
Peter
P
Benhaim
Prosper
P
Hedrick
Mare H
MH
Fraser
John K
JK
D000273:Adipose Tissue / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans
D000954:Antigens, Surface