title: Oxidative DNA damage in peripheral leukocytes and its association with expression and polymorphisms of hOGG1: a study of adolescents in a high risk region for hepatocellular carcinoma in China.
abstract: OBJECTIVE
  To study the oxidative DNA damage to adolescents of hepatocellular carcinoma (HCC) families in Guangxi Zhuang Autonomous Region, China.
  
  
  METHODS
  Peripheral leukocytes' DNA 7, 8-dihydro-8-oxoguanine (8-oxoG) and repair enzyme hOGG1 were quantified by flow-cytometry. hOGG1-Cys326Ser single nucleotide polymorphism (SNP) was distinguished by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) assay.
  
  
  RESULTS
  There was a positive correlation between 8-oxoG and repair enzyme hOGG1 expression (P<0.001). HCC children (n=21) in Fusui county had a higher level of hOGG1 (P<0.01) and a lower level of 8-oxoG (P<0.05) than the controls (n=63) in Nanning city. Children in Nanning exposed to passive-smoking had a higher hOGG1 expression (P<0.05) than the non-exposers. 8-oxoG and hOGG1 were negatively correlated with body mass index, while hOGG1 was positively correlated with age. There was a peak of 8-oxoG level nearby the 12 year point. Individuals with the hOGG1 326Ser allele had a significantly marginal higher concentration of leukocyte 8-oxoG level than hOGG1 326Cys allele.
  
  
  CONCLUSIONS
  This is the first report using flow-cytometry to simultaneously quantify both the DNA oxidative damage and its repairing enzyme hOGG1. The results provide new insights towards a better understanding of the mechanisms of oxidative stress in a population highly susceptible to hepatocarcinogenesis.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China. pengpang@hotmail.com
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Han-Ming
authors.initials: HM
authors.lastname: Shen
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Liu
authors.forename: Lu-Nan
authors.initials: LN
authors.lastname: Yan
authors.forename: Min-Hao
authors.initials: MH
authors.lastname: Peng
authors.forename: Le-Qun
authors.initials: LQ
authors.lastname: Li
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Liang
authors.forename: Zong-Liang
authors.initials: ZL
authors.lastname: Wei
authors.forename: Barry
authors.initials: B
authors.lastname: Halliwell
authors.forename: Choon Nam
authors.initials: CN
authors.lastname: Ong
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015415:Biomarkers; D015992:Body Mass Index; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000378:metabolism*; D002681:China / Q000453:epidemiology; D004249:DNA Damage / Q000276:immunology*; D045647:DNA Glycosylases / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005838:Genotype; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism*; D008297:Male; D015999:Multivariate Analysis; D018384:Oxidative Stress; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C024829:8-hydroxyguanine; D006147:Guanine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human