title: Metabolic analysis of acetate accumulation during xylose consumption by Paenibacillus polymyxa.
abstract: Paenibacillus polymyxa ATCC 12321 produced more acetic acid and less butanediol from xylose than from glucose. The product yields from xylose were ethanol (0.72 mol/mol sugar), (R,R)-2,3-butanediol (0.31 mol/mol sugar), and acetate (0.38 mol/mol sugar) while those from glucose were ethanol (0.74 mol/mol sugar), (R,R)-2,3-butanediol (0.46 mol/mol sugar), and acetate (0.05 mol/mol sugar). Higher acetate kinase activity and lower acetate uptake ability were found in xylose-grown cells than in glucose-grown cells. Furthermore, phosphoketolase activity was higher in xylose-grown cells than in glucose-grown cells. In fed-batch culture on xylose, glucose feeding raised the butanediol yield to 0.56 mol/mol sugar and reduced acetate accumulation to 0.04 mol/mol sugar.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, Graduate School of Advanced Sciences of Matter, 1-3-1, 739-8530 Kagamiyama, Higashi-Hiroshima, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Marwoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashimada
authors.forename: T
authors.initials: T
authors.lastname: Kakizono
authors.forename: N
authors.initials: N
authors.lastname: Nishio
mesh_terms: D000084:Acetate Kinase / Q000378:metabolism*; D000085:Acetates / Q000032:analysis* / Q000378:metabolism; D000093:Acetoin / Q000032:analysis; D000446:Aldehyde-Lyases / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism; D018533:Biomass; D002072:Butylene Glycols / Q000032:analysis / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D005561:Formates / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D016936:Gram-Positive Endospore-Forming Bacteria / Q000378:metabolism*; D006859:Hydrogen / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000032:analysis; D010427:Pentose Phosphate Pathway; D019802:Succinic Acid / Q000032:analysis; D014994:Xylose / Q000378:metabolism*
chemical_list: D000085:Acetates; D002072:Butylene Glycols; D003470:Culture Media; D005561:Formates; C030544:formic acid; D002245:Carbon Dioxide; D019344:Lactic Acid; D000431:Ethanol; D006859:Hydrogen; D014994:Xylose; D019802:Succinic Acid; D000093:Acetoin; D000084:Acetate Kinase; D000446:Aldehyde-Lyases; C022367:phosphoketolase; D005947:Glucose