title: An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities.
abstract: We propose a strategy for the efficient screening of large libraries of amplified 16S rRNA genes from complex environmental samples. It consists of processing sets of multiple clones simultaneously. This strategy saves up to 90% of the costs and labor spent in the process of screening a 16S rDNA library.
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Científicas, P.O. Box 1052, 41080, Seville, Spain. jmgrau@irnase.csic.es
authors.forename: J M
authors.initials: JM
authors.lastname: Gonzalez
authors.forename: A
authors.initials: A
authors.lastname: Ortiz-Martinez
authors.forename: M A
authors.initials: MA
authors.lastname: Gonzalez-delValle
authors.forename: L
authors.initials: L
authors.lastname: Laiz
authors.forename: C
authors.initials: C
authors.lastname: Saiz-Jimenez
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D015723:Gene Library; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S