title: Wound healing of 6.45-microm free electron laser skin incisions with heat-conducting templates.
abstract: We have previously shown a reduction in lateral thermal damage with acute studies of skin incisions made in vitro using heat-conducting templates. Here we examined the wound-healing response to laser incisions with heat-conducting templates and explored the use of an optically transparent template with the free electron laser (FEL) at 6.45 microm. First we evaluated the effects of a sapphire heat-conducting template on the lateral thermal damage of FEL incisions using in vitro human skin samples. Next we compared wound tensile strength and histological scoring of the healing of incisions created on the dorsal pelts of live rats with the FEL utilizing metal and sapphire heat-conducting templates and scalpel incisions. The animals were euthanized and the wounds were analyzed at postoperative days 7, 14, and 21. The depth and lateral thermal damage of FEL incisions on in vitro human skin were significantly reduced with the sapphire heat-conducting template. Nonstatistically significant differences in wound tensile strengths and histological scoring of wound healing were noted at days 7 and 14. By day 21, all of the incisions appeared similar. When the data from days 7 and 14 were combined, statistically significant differences were found for each of the templates (except the histological evaluation with the aluminum template) and the scalpel compared with laser incisions made without using a template. The use of metal or sapphire heat-conducting templates reduced the wound-healing delay of laser incisions seen at postoperative days 7 and 14.
authors.affiliation: Vanderbilt University, Department of Medicine, Division of Dermatology, Nashville, Tennessee 37232-5227, USA.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Robbins
authors.forename: Lou
authors.initials: L
authors.lastname: Reinisch
authors.forename: Darrel L
authors.initials: DL
authors.lastname: Ellis
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology* / Q000503:physiopathology / Q000517:prevention & control*; D062109:Dermatologic Surgical Procedures; D004867:Equipment Design; D004868:Equipment Failure; D006801:Humans; D053685:Laser Therapy / Q000009:adverse effects* / Q000295:instrumentation* / Q000379:methods; D011832:Radiation Injuries / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D011835:Radiation Protection / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology* / Q000528:radiation effects; D013814:Thermal Conductivity; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects