Supercritical fluid disruption of Ralstonia eutropha for poly(beta-hydroxybutyrate) recovery.
A new method for disruption of Gram-negative bacterium Ralstonia eutropha by supercritical CO(2) for poly(beta-hydroxybutyrate) (PHB) recovery is proposed. The effects of different parameters such as exposure time, pressure, temperature, volume of methanol as a modifier, and culture history on cell disruption efficiency were investigated using Taguchi's statistical approach to determine optimum conditions. The optimum conditions for cell disruption and PHB recovery were as follows: exposure time, 100 min; pressure, 200 atm; temperature, 40 degrees C; volume of methanol, 0.2 mL. The cell culture time was less significant. At optimum conditions, the maximum efficiency of PHB recovery was found to be 89%. The proposed method is comparable with other recovery methods in terms of the percentage of PHB recovery, while it is environmentally more benign.
Hejazi
Parisa
P
Department of Chemical Engineering, Tarbiat Modarres University, P.O. Box 14115-143, Tehran, Iran.
Vasheghani-Farahani
Ebrahim
E
Yamini
Yadollah
Y
D018929:Cell Culture Techniques / Q000379:methods*; D002458:Cell Fractionation / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D020118:Cupriavidus necator / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000302:isolation & purification* / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D011091:Polyesters / Q000302:isolation & purification* / Q000378:metabolism*; D011312:Pressure; D013696:Temperature; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D000432:Methanol; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates