[Induction of nuclear envelope formation around individual chromosomes under impact of hypotonic shock].
In the present work we have studied the distribution of some proteins participating in the nuclear envelope assembly (lamins A/C, B and LAP2 alpha) in mitotic cells and after hypotonic treatment with 15% Hank's solution. In untreated cells, these proteins are localized in the nuclei of interphase cells migrate to the cytoplasm during mitosis. Hypotonic treatment of interphase, prophase and telophase cells does not lead to considerable relocalization of lamins A/C and B. However, unlike normal mitosis, in prometaphase and metaphase cells their chromosomes acquire affinity to lamins and LAP2 alpha. Comparative analysis of lamins and LAP2 alpha distribution have revealed that chromosomes have special sites for binding with different proteins.
Kurchashova
S Iu
SIu
Department of Electron Microscopy, Research Institute of Physico-Chemical Biology after A. N. Belozersky, Moscow State University.
Filimonenko
V V
VV
Gulak
P V
PV
Kireev
I I
II
Poliakov
V Iu
VIu
Hozak
P
P
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007038:Hypotonic Solutions; D007399:Interphase; D034904:Lamin Type A / Q000378:metabolism; D034921:Lamin Type B / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008938:Mitosis; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism*; D009994:Osmolar Concentration
D004268:DNA-Binding Proteins; D007038:Hypotonic Solutions; D034904:Lamin Type A; D034921:Lamin Type B; D008565:Membrane Proteins; D009687:Nuclear Proteins; C440308:lamin C; C093413:lamina-associated polypeptide 2