Crystallization and preliminary X-ray diffraction study of hemoglobin D from the Aldabra giant tortoise, Geochelone gigantea.
Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 A resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta = 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).
Kuwada
Takao
T
Institute of Quantum Science, Nihon University, Funabashi 274-8501, Japan.
Hasegawa
Tomokazu
T
Satoh
Isamu
I
Ishikawa
Koichi
K
Shishikura
Fumio
F
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D003627:Data Interpretation, Statistical; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000302:isolation & purification; D014426:Turtles
D006455:Hemoglobins, Abnormal; C032001:hemoglobin D