[Streptomyces griseolus # 182--a novel organism producing oligomycin antibiotics. Taxonomy, fermentation, and isolation].
Target screening of natural immunosuppressors resulted in isolation of a strain of Streptomyces griseolus (No. 182) producing a complex of antifungal antibiotics. The strain proved to be an aerobe with the growth temperature of 26 to 28 degrees C. Morphological features and physiological properties of the strain were studied. Scanning electron microscopy revealed smooth, oval spores 1.10-1.25 mu in size. The findings showed that the strain belonged to Streptomyces griseolus. Unlike the previously described organisms producing the oligomycin complex the new strain formed straight or twisted sporophores and did not produce melanoid pigment or soluble pigment when grown on the Gauze mineral agar medium No. 1. The procedures for biosynthesis and chemical recovery of the antibiotic complex from the mycelium are described. The complex was shown to include 3 components at a ratio of 80:15:5 identified as oligomycins A, B and C respectively. The oligomycin complex was highly active against Aspergillus niger 137, Tolypocladium inflatum, Fusarium ocsisporum, Curvularia lunata 645 and Trichoderma alba F-32 (MIC 0.1-1.0 mcg/ml). The activity against yeast and bacterial cultures was observed only when the doses were higher than 100 mcg/ml.
Grammatikova
N E
NE
National Research Institute of Antibiotics, Moscow.
Bibikova
M V
MV
Spiridonova
I A
IA
Kabanov
A E
AE
Katlinskiĭ
A V
AV
D000935:Antifungal Agents / Q000096:biosynthesis* / Q000494:pharmacology; D005285:Fermentation; D008854:Microscopy, Electron; D009840:Oligomycins / Q000096:biosynthesis* / Q000494:pharmacology; D013302:Streptomyces / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism
D000935:Antifungal Agents; D009840:Oligomycins; C031004:oligomycin A; C048335:oligomycin B; C042075:oligomycin C