title: A catalogue of molecular, physiological and symbiotic properties of soybean-nodulating rhizobial strains from different soybean cropping areas of China.
abstract: We have analysed 198 fast-growing soybean-nodulating rhizobial strains from four different regions of China for the following characteristics: generation time; number of plasmids; lipopolysaccharide (LPS), nodulation factors (LCOs) and PCR profiles; acidification of growth medium; capacity to grow at acid, neutral, and alkaline pH; growth on LC medium; growth at 28 and 37 degrees C; melanin production capacity; Congo red absorption and symbiotic characteristics. These unbiased analyses of a total subset of strains isolated from specific soybean-cropping areas (an approach which could be called "strainomics") can be used to answer various biological questions. We illustrate this by a comparison of the molecular characteristics of five strains with interesting symbiotic properties. From this comparison we conclude, for instance, that differences in the efficiency of nitrogen fixation or competitiveness for nodulation of these strains are not apparently related to differences in Nod factor structure.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, UK.
authors.forename: J
authors.initials: J
authors.lastname: Thomas-Oates
authors.forename: J
authors.initials: J
authors.lastname: Bereszczak
authors.forename: E
authors.initials: E
authors.lastname: Edwards
authors.forename: A
authors.initials: A
authors.lastname: Gill
authors.forename: S
authors.initials: S
authors.lastname: Noreen
authors.forename: J C
authors.initials: JC
authors.lastname: Zhou
authors.forename: M Z
authors.initials: MZ
authors.lastname: Chen
authors.forename: L H
authors.initials: LH
authors.lastname: Miao
authors.forename: F L
authors.initials: FL
authors.lastname: Xie
authors.forename: J K
authors.initials: JK
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: S S
authors.initials: SS
authors.lastname: Yang
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: H P
authors.initials: HP
authors.lastname: Spaink
authors.forename: H R M
authors.initials: HR
authors.lastname: Schlaman
authors.forename: M
authors.initials: M
authors.lastname: Harteveld
authors.forename: C L
authors.initials: CL
authors.lastname: Díaz
authors.forename: A A N
authors.initials: AA
authors.lastname: van Brussel
authors.forename: M
authors.initials: M
authors.lastname: Camacho
authors.forename: D N
authors.initials: DN
authors.lastname: Rodríguez-Navarro
authors.forename: C
authors.initials: C
authors.lastname: Santamaría
authors.forename: F
authors.initials: F
authors.lastname: Temprano
authors.forename: J M
authors.initials: JM
authors.lastname: Acebes
authors.forename: R A
authors.initials: RA
authors.lastname: Bellogín
authors.forename: A M
authors.initials: AM
authors.lastname: Buendía-Clavería
authors.forename: M T
authors.initials: MT
authors.lastname: Cubo
authors.forename: M R
authors.initials: MR
authors.lastname: Espuny
authors.forename: A M
authors.initials: AM
authors.lastname: Gil
authors.forename: R
authors.initials: R
authors.lastname: Gutiérrez
authors.forename: A
authors.initials: A
authors.lastname: Hidalgo
authors.forename: F J
authors.initials: FJ
authors.lastname: López-Baena
authors.forename: N
authors.initials: N
authors.lastname: Madinabeitia
authors.forename: C
authors.initials: C
authors.lastname: Medina
authors.forename: F J
authors.initials: FJ
authors.lastname: Ollero
authors.forename: J M
authors.initials: JM
authors.lastname: Vinardell
authors.forename: J E
authors.initials: JE
authors.lastname: Ruiz-Sainz
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D002681:China; D003224:Congo Red / Q000378:metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis; D008543:Melanins / Q000096:biosynthesis; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012231:Rhizobium / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D013025:Glycine max / Q000382:microbiology*; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D008070:Lipopolysaccharides; D008543:Melanins; D012336:RNA, Ribosomal, 16S; C023023:lipid-linked oligosaccharides; D003224:Congo Red