title: Comparison of developmental endothelial locus-1 angiogenic factor with vascular endothelial growth factor in a porcine model of cardiac ischemia.
abstract: BACKGROUND
  This study compared the angiogenic effects of developmental endothelial locus-1 (DEL-1), vascular endothelial growth factor (VEGF), as well as the negative control, beta-galactosidase (beta-gal), in a porcine model of cardiac ischemia.
  
  
  METHODS
  Twenty pigs underwent left circumflex artery occlusions. After 3 weeks, the animals received myocardial injections of adenovirus expressing beta-gal (n=6), DEL-1 (n=7), or VEGF (n=7). At 7 weeks, animals were assessed for both function and coronary flow and compared with baseline measurements.
  
  
  RESULTS
  Regional wall motion index and global ejection fraction showed deterioration in function in the beta-gal group and no change in the VEGF and DEL-1 groups between the treatment and harvest time points. Preload recruitable stroke work suggested functional improvement in the VEGF group (35.8 +/- 8.6 vs 56.4 +/- 17.8, p = 0.033). The increase in the DEL-1 group was not statistically significant (27.3 +/- 9.8 vs, 40.2 +/- 19.4, p = 0.067). The beta-gal group exhibited minimal change (30.7 +/- 14.8 vs 35.9 +/- 12.1, p = 0.96). Regional blood flow as assessed by fluorescent microspheres was improved under stress conditions in the VEGF group (1.00 +/- 0.15 vs 1.15 +/-0.22, p = 0.03).
  
  
  CONCLUSIONS
  Treatment with VEGF led to a modest improvement in regional blood flow and cardiac function in previously ischemic myocardial tissue.
authors.affiliation: Department of Cardiothoracic Surgery, Stanford University School of Medicine, California 94305, USA.
authors.forename: Murray H
authors.initials: MH
authors.lastname: Kown
authors.forename: Takeshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Mark L
authors.initials: ML
authors.lastname: Koransky
authors.forename: Kalyani
authors.initials: K
authors.lastname: Penta
authors.forename: Gordon
authors.initials: G
authors.lastname: Sakamoto
authors.forename: Christina L
authors.initials: CL
authors.lastname: Jahncke
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Carter
authors.forename: Thomas
authors.initials: T
authors.lastname: Quertermous
authors.forename: Robert C
authors.initials: RC
authors.lastname: Robbins
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D017023:Coronary Angiography; D003326:Coronary Circulation / Q000187:drug effects; D005260:Female; D005787:Gene Frequency; D017202:Myocardial Ischemia / Q000503:physiopathology*; D013552:Swine; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D016277:Ventricular Function, Left / Q000187:drug effects / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D042461:Vascular Endothelial Growth Factor A