Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation.
We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.
Taga
Masatoki
M
Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. mtaga@cc.okayama-u.ac.jp
Tsuchiya
Dai
D
Murata
Minoru
M
D001203:Ascomycota / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D002453:Cell Cycle; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics; D006999:Hypocreales / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D009690:Nucleic Acid Conformation; D013045:Species Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal