Performance prediction of industrial centrifuges using scale-down models.
Computational fluid dynamics was used to model the high flow forces found in the feed zone of a multichamber-bowl centrifuge and reproduce these in a small, high-speed rotating disc device. Linking the device to scale-down centrifugation, permitted good estimation of the performance of various continuous-flow centrifuges (disc stack, multichamber bowl, CARR Powerfuge) for shear-sensitive protein precipitates. Critically, the ultra scale-down centrifugation process proved to be a much more accurate predictor of production multichamber-bowl performance than was the pilot centrifuge.
Boychyn
M
M
The Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Yim
S S S
SS
Bulmer
M
M
More
J
J
Bracewell
D G
DG
Hoare
M
M
D001773:Blood Cells / Q000166:cytology; D001798:Blood Proteins / Q000302:isolation & purification*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003198:Computer Simulation; D005656:Fungal Proteins / Q000302:isolation & purification*; D006801:Humans; D007221:Industry / Q000379:methods; D044085:Microfluidics / Q000379:methods; D008956:Models, Chemical; D010865:Pilot Projects; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000379:methods*
D001798:Blood Proteins; D005656:Fungal Proteins