Genosnip: SNP genotyping by MALDI-TOF MS using photocleavable oligonucleotides.
A photocleavable o-nitrobenzyl CE phosphoramidite building-block was synthesised and incorporated within oligonucleotides. After allele-specific primer extension, desalting was performed using genostrep purification plates. Release of the SNP information containing part through photocleavage created shortened molecules that are easily accessible for MALDI-TOF analysis. Additionally, incorporation of mass modified nucleosides enables flexible design of multiplex genotyping.
Wenzel
T
T
Bruker Daltonik GmbH, Leipzig, Germany. tw@bsax.de
Elssner
T
T
Fahr
K
K
Bimmler
J
J
Richter
S
S
Thomas
I
I
Kostrzewa
M
M
D005165:Factor V / Q000235:genetics; D005838:Genotype; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry*; D010782:Photolysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011516:Prothrombin / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D009841:Oligonucleotides; C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin