Stabilization of DNA double and triple helices by conjugation of minor groove binders to oligonucleotides.
New conjugates containing two parallel or antiparallel carboxamide minor groove binders (MGB) attached to the same terminal phosphate of one oligonucleotide strand were synthesized. The conjugates interact with their target DNA stronger than the individual components. Effect of conjugated MGB on DNA duplex and triplex stability and their sequence specificity was demonstrated on the short oligonucleotide duplexes and on the triplex formed by model 16-mer oligonucleotide with HIV polypurine tract.
Boutorine
A S
AS
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U 565, CNRS UMR 8646, Paris Cedex, France. boutorin@mnhn.fr
Ryabinin
V A
VA
Novopashina
D S
DS
Venyaminova
A G
AG
Hélène
C
C
Sinyakov
A S
AS
D020029:Base Pairing; D001665:Binding Sites; D003596:Cytosine; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D006147:Guanine; D006678:HIV / Q000235:genetics; D007202:Indicators and Reagents; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D004279:DNA, Viral; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D006147:Guanine; D003596:Cytosine; D004247:DNA