title: Development of a long-lasting ventricular catheter impregnated with a combination of antibiotics.
abstract: A ventricular silicone catheter impregnated with a combination of rifampin and a quinolone was developed in order to prevent ventricular shunt related infections. As model substance for the quinolones we used sparfloxacin, because of its specific physicochemical properties resulting in a quantitative detection also in the presence of a second antibiotic. In our study we focused especially on an optimization of the antibiotic release out of the impregnated catheters in order to develop long lasting devices with a broad antimicrobial spectrum. A release-optimized catheter was tested with an in vitro colonization test and additionally with a method developed to examine the spread of bacteria on a catheter surface. In vitro experiments showed that the impregnated catheters reduce the colonization with Staphylococcus epidermidis for at least 1 year and prevent the spread of bacteria along the catheter surface.
authors.affiliation: Department of Hygiene and Environmental Medicine, Johannes Gutenberg-University of Mainz, Hochhaus am Augustusplatz, D-55131 Mainz, Germany. kohnen@mail.uni-mainz.de
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kohnen
authors.forename: Christian
authors.initials: C
authors.lastname: Kolbenschlag
authors.forename: Susanne
authors.initials: S
authors.lastname: Teske-Keiser
authors.forename: Bernd
authors.initials: B
authors.lastname: Jansen
mesh_terms: D000900:Anti-Bacterial Agents; D002408:Catheters, Indwelling; D002552:Cerebral Ventricles / Q000187:drug effects* / Q000382:microbiology / Q000601:surgery*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000627:therapeutic use*; D016503:Drug Delivery Systems / Q000379:methods*; D004343:Drug Implants / Q000494:pharmacology / Q000627:therapeutic use; D004359:Drug Therapy, Combination / Q000008:administration & dosage* / Q000737:chemistry; D004866:Equipment Contamination / Q000517:prevention & control; D019544:Equipment Failure Analysis / Q000379:methods*; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D015363:Quinolones / Q000008:administration & dosage / Q000737:chemistry; D012293:Rifampin / Q000008:administration & dosage / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013242:Sterilization / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D004343:Drug Implants; D015363:Quinolones; D012293:Rifampin