title: Was elusive carnivore a panther? DNA typing of faeces reveals the mystery.
abstract: In this study, we report the findings of a recent case in which the officials of an Indian zoo claimed that an animal, possibly a carnivore, is periodically visiting the zoo from a nearby vast forest area and causing panic in zoo and nearby villages. They collected some elusive faecal material from the vicinity of an herbivore enclosure. Looking to the pugmarks found in that area and faecal matter ceased, the officials could not decide whether it was a lioness, a tiger or a panther. We resolved this mystery by DNA-based analysis of the faecal material, using our recently developed novel universal primers to amplify and sequence a specific fragment of mitochondrial cytochrome b gene. The findings of the DNA-based analyses were confirmed after few days when the zoo officials trapped the animal of same species as suggested in our report. The potential of our procedure to investigate the cases related to wildlife offence is discussed.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Verma
authors.forename: Kasturi
authors.initials: K
authors.lastname: Prasad
authors.forename: Narayan
authors.initials: N
authors.lastname: Nagesh
authors.forename: Mehar
authors.initials: M
authors.lastname: Sultana
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
mesh_terms: D000818:Animals; D002335:Carnivora / Q000235:genetics*; D003573:Cytochrome b Group / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers; D005243:Feces / Q000737:chemistry*; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D003573:Cytochrome b Group; D017931:DNA Primers; D004247:DNA