Experimental hypersensitivity pneumonitis: role of MCP-1.
Inhalation of Saccharopolyspora rectivirgula causes "farmer's lung" disease, a classic example of hypersensitivity pneumonitis (HP). Monocyte chemoattractant protein-1 (MCP-1) is increased in the bronchoalveolar lavage fluid of mice challenged with S rectivirgula, and S rectivirgula induces MCP-1 secretion by alveolar macrophages. We tested the hypothesis that MCP-1 and its receptor CC chemokine receptor-2 (CCR2) are essential to the development of experimental HP by treating mice with MCP-1 antibody and using CCR2(-/-) mice. Administration of anti-MCP-1 did not change the response to intratracheally administered S rectivirgula. CCR2(-/-) animals responded in a fashion similar to that of wild-type animals to intratracheally administered.S rectivirgula. To determine the influence of the MCP-1-CCR2 interaction in vitro, we transferred S rectivirgula-cultured spleen cells from S rectivirgula-sensitized mice, to naïve recipients. Later, challenge of the recipients with intratracheal S rectivirgula and examination of both lung histology and bronchoalveolar lavage fluid characteristics were used to determine whether adoptive transfer had occurred. We found that cultured cells from CCR2(-/-) animals were fully capable of adoptive transfer. We conclude that interaction of MCP-1 with CCR2 is not necessary for the development of pulmonary inflammation in response to intratracheally administered S rectivirgula or cells able to adoptively transfer experimental HP.
Schuyler
Mark
M
Department of Medicine, Albequerque Veterans Affairs Medical Center, University of New Mexico, 87108, USA.
Gott
Katherine
K
Cherne
Amy
A
D019264:Adoptive Transfer; D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism*; D005203:Farmer's Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D016930:Saccharopolyspora; D013154:Spleen / Q000166:cytology
D000906:Antibodies; C515335:Ccr2 protein, mouse; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D019707:Receptors, Chemokine