title: The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC).
abstract: The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. YnfI (dmsD) has no dms paralogue. YnfE/F and YnfG could be detected by immunoblotting with anti-DmsAB antibodies when expressed under the control of a tac or dms promoter. Cells harbouring ynfFGH on a multicopy plasmid supported anaerobic growth with dimethyl sulfoxide (DMSO) as respiratory oxidant in a dmsABC deletion, suggesting that YnfFGH forms a heterotimeric enzyme complex similar to DmsABC. Exchange of DmsC by YnfH (DmsAB-YnfH) resulted in membrane localization, anaerobic growth on DMSO, and binding of 2-n-heptyl 4-hydroxyquinoline-N-oxide, indicating that YnfH was a competent anchor. YnfG can also replace DmsB as the electron transfer subunit and assembled [Fe-S] clusters as judged by electron paramagnetic resonance spectroscopy. YnfE and/or YnfF could not form a functional complex with DmsBC and expression of YnfE prevented the accumulation of YnfFGH.
authors.affiliation: CIHR Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Alta., T6G 2H7, Edmonton, Canada.
authors.forename: Shannon P
authors.initials: SP
authors.lastname: Lubitz
authors.forename: Joel H
authors.initials: JH
authors.lastname: Weiner
mesh_terms: D000693:Anaerobiosis / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000378:metabolism*; D004925:Escherichia / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007506:Iron-Sulfur Proteins; D009876:Operon / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D007506:Iron-Sulfur Proteins; D010455:Peptides; D011994:Recombinant Proteins; D010088:Oxidoreductases; C045664:dimethyl sulfoxide reductase; D004121:Dimethyl Sulfoxide