Cardiovascular, haematological and neurological effects of the venom of the Papua New Guinean small-eyed snake (Micropechis ikaheka) and their neutralisation with CSL polyvalent and black snake antivenoms.
Cardiovascular and haematological effects of venom of the small-eyed Snake (Micropechis ikaheka) were examined in ventilated anaesthetised piglets. Neurotoxic effects were examined in chick biventer cervicis nerve-muscle preparations. Immunoreactivity of venom was tested against the monovalent antivenom components in a CSL Ltd Venom Detection Kit. Neutralisation was tested in vivo and in vitro with CSL Ltd polyvalent snake and Black Snake (Pseudechis australis) antivenoms. Venom in 0.1% bovine serum albumin in saline was infused into piglets in doses 1-2000 microg/kg. Pulmonary hypertension (P= 0.0007) and depression of cardiac output (P= 0.002) were observed up to 3 h after 150-160 microg/kg. The concentration of plasma free-haemoglobin increased more than 50-fold, indicating haemolysis. Neither coagulopathy nor thrombocytopenia occurred. Creatine phosphokinase and serum potassium levels did not increase suggesting absence of acute rhabdomyolysis. The venom caused post-synaptic neurotoxicty. Immunoreactivity of venom with Black Snake antivenom was observed at very high venom concentrations. Cardiovascular effects were absent and haemolysis was less after venom was pre-incubated at 37 degrees C for 30 min with polyvalent antivenom. Neutralisation by Black Snake antivenom was less effective. The neurotoxicity was neutralised by polyvalent or Black Snake antivenoms. Human envenomation may be treated with CSL Ltd polyvalent snake antivenom.
Australian Venom Research Unit, Department of Pharmacology, University of Melbourne, Melbourne, Vic. 3010, Australia. james.tibballs@rch.org.au
J
J
Tibballs
S
S
Kuruppu
W C
WC
Hodgson
T
T
Carroll
G
G
Hawdon
M
M
Sourial
T
T
Baker
K
K
Winkel
D000818:Animals; D000997:Antivenins / Q000276:immunology / Q000627:therapeutic use; D002302:Cardiac Output / Q000187:drug effects*; D004546:Elapid Venoms / Q000276:immunology / Q000633:toxicity*; D017815:Elapidae; D006461:Hemolysis / Q000187:drug effects*; D006976:Hypertension, Pulmonary / Q000139:chemically induced*; D009498:Neurotoxins / Q000276:immunology / Q000633:toxicity*; D012909:Snake Bites / Q000097:blood / Q000276:immunology; D013552:Swine
D000997:Antivenins; D004546:Elapid Venoms; D009498:Neurotoxins