Changes in the expression of calbindin D-28k in the gerbil hippocampus following seizure.
Previous studies have reported that calbindin D-28k (CB), a calcium-binding protein, containing neurons in the hippocampus play an important role in hippocampal excitability in epilepsy, because CB modulates the free calcium ion during seizure. Hence, in the present study, we investigated changes of CB expression in the hippocampus and its association in the Mongolian gerbil to identify roles of CB in epileptogenesis. CB immunoreactivity in the hippocampus was significantly lower in the pre-seizure group of seizure sensitive (SS) gerbils as compared with those seen in the seizure resistant (SR) gerbils. The distribution of CB immunoreactivity in the hippocampus showed significant difference after seizure on-set in SS gerbils. CB immunoreactivity in the hippocampal CA1, CA2 areas, and subiculum was lowest at 3h after seizure on-set; thereafter, the immunoreactivity became to increase to 12h after seizure on-set. Mossy fibers, Schaffer collaterals and dentate granule cells showed the highest CB immunoreactivity at 3h after seizure on-set; thereafter, the immunoreactivity became to decrease. In the case of the intrinsic and output connections of the hippocampus, a rapid decrease of CB serves an inhibitory function, which regulates the seizure activity and output signals from the hippocampus.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine, Hallym University, Kangwon-Do, 200-702, Chunchon, South Korea.
Nam
Young Sam
YS
Chung
Dae Won
DW
Lee
Heungshik S
HS
Yoon
Yeo Sung
YS
Yoo
Ki-Yeon
KY
Kang
Tae-Cheon
TC
Lee
In Se
IS
Won
Moo Ho
MH
D000818:Animals; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism / Q000473:pathology; D017966:Pyramidal Cells / Q000378:metabolism / Q000473:pathology; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis*; D012640:Seizures / Q000378:metabolism* / Q000473:pathology
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D002118:Calcium