Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Exosomes are 30-100 nm diameter vesicles formed by inward budding of endosomal compartments and are produced by several cell types, including T-cells, B-cells and dendritic cells (DC)s. Exosomes from DCs express major histocompatibility complexes (MHC) class I and II, and co-stimulatory molecules on their surface, and can induce antigen-specific activation of T-cells. The aims of the present study were to investigate for the presence of exosomes in bronchoalveolar lavage fluid (BALF) from healthy individuals, and to establish if these exosomes bear MHC and co-stimulatory molecules. The authors analysed BALF taken from seven healthy volunteers and used exosomes from monocyte-derived DC (MDDC) cultures as a reference. After ultracentrifugation, exosomes were bound to anti-MHC class II coated magnetic beads and analysed by flow cytometry and electron microscopy. The authors report for the first time that exosomes are present in BALF. These exosomes are similar to MDDC derived exosomes as they express MHC class I and II, CD54, CD63 and the co-stimulatory molecule CD86. The results demonstrate that exosomes are present in the lung, and since they contain both major histocompatibility complex and co-stimulatory molecules it is likely that they are derived from antigen presenting cells and might have a regulatory role in local immune defence.
Dept of Medicine, Unit of Clinical Allergy Research, Karolinska Hospital and Institutet, Stockholm, Sweden. Charlotte.Admyre@cfa.ki.se
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Admyre
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Grunewald
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Thyberg
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Gripenbäck
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Tornling
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Eklund
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Scheynius
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Gabrielsson
D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D022162:Cytoplasmic Vesicles / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D005802:Genes, MHC Class II / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D015703:Antigens, CD; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I