Expression of receptor for advanced glycation end products (RAGE) in human biliary cancer cells.
OBJECTIVE
Amphoterin is considered as a regulator for the ability of invasion and migration in tumor cells and embryonic neurons through binding to RAGE (receptor for advanced glycation end products), a mult-iligand cell surface molecule of the immunoglobulin superfamily.


METHODS
To see the involvement of RAGE in biliary cancer, three representative human biliary cancer cells (SK-ChA-1, TGBC-1 and NOZC-1) were rendered for the study. Cell invasion ability was determined using Matrigel invasion assay. The expression of RAGE protein was studied by Western blotting.


RESULTS
Cell invasion assay through Matrigel showed high invasion potential in SK-ChA-1 and NOZC-1 (40.3 +/- 3.27, 48.7 +/- 4.8); low invasion potential in TGBC-1 (25.7 +/- 2.8). RAGE was strongly expressed in SK-ChA-1 and NOZC-1 that have high invasion ability. On the contrary, RAGE was faintly expressed in TGBC-1 that has low ability.


CONCLUSIONS
RAGE is expressed in concordance to the invasion ability of the human biliary cancer cells. Control of this molecule could be a key to regulate the invasion ability of biliary cancers.
Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kobe University, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Kenro
K
Hirata
Moriatsu
M
Takada
Yasuyuki
Y
Suzuki
Yoshikazu
Y
Kuroda
D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001672:Biocompatible Materials; D003094:Collagen; D004338:Drug Combinations; D005706:Gallbladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007797:Laminin; D009361:Neoplasm Invasiveness; D011509:Proteoglycans; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D001672:Biocompatible Materials; D004338:Drug Combinations; D017127:Glycation End Products, Advanced; D007797:Laminin; D011509:Proteoglycans; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C059485:matrigel; D003094:Collagen