Expression of heme oxygenase-1 and regulation by cytokines in human osteoarthritic chondrocytes.
Heme oxygenase-1 (HO-1) is implicated in the protection against tissue injury. We investigated the expression of this protein in cartilage sections and chondrocytes obtained from osteoarthritic patients. HO-1 was immunodetected in preparations from cartilage and also in chondrocytes cultured in the absence of stimulation. We found that HO-1 can be modulated by cytokines since the pro-inflammatory cytokines interleukin (IL)-1beta, IL-17 and tumour necrosis factor-alpha (TNF-alpha) down-regulated this protein, whereas the anti-inflammatory cytokine IL-10 exerted the opposite effect. Our results suggest a role for HO-1 as part of protective mechanisms against tissue injury in human cartilage.
Departamento de Farmacología, Universidad de Valencia, Avda. Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain.
Patricia
P
Fernández
Maria Isabel
MI
Guillén
Francisco
F
Gomar
Maria José
MJ
Alcaraz
D000368:Aged; D019902:Chondrocytes / Q000187:drug effects* / Q000201:enzymology; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics*; D051547:Heme Oxygenase-1; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D020381:Interleukin-17 / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins; D010003:Osteoarthritis / Q000473:pathology*
D016207:Cytokines; D007375:Interleukin-1; D020381:Interleukin-17; D008565:Membrane Proteins; D016753:Interleukin-10; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1