Flattening Drosophila cells for high-resolution light microscopic studies of mitosis in vitro.
Here we briefly review techniques used to flatten cells that otherwise round in culture, so that their division can be more clearly analyzed in vitro by high resolution light microscopy. We then describe an agar overlay procedure for use with isolated Drosophila neuroblasts, which promotes their long-term viability while also allowing for correlative studies of the same cell in the living and fixed state. This same procedure can also be used to obtain high temporal and spatial resolution images of mitosis and cytokinesis in cultured Drosophila Schneider S2 cells, which are a popular model for RNAi studies.
Fleming
Shawna L
SL
Division of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509, USA.
Rieder
Conly L
CL
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004330:Drosophila / Q000166:cytology* / Q000648:ultrastructure; D008853:Microscopy / Q000379:methods; D008938:Mitosis; D009474:Neurons / Q000648:ultrastructure*; D012685:Sepharose
D012685:Sepharose