Stimulation of betacyanin synthesis through exogenous methyl jasmonate and other elicitors in suspension-cultured cells of Portulaca.
Betacyanin production in suspension-cultured cells of Portulaca was significantly enhanced by both abiotic and biotic elicitors. Betacyanin levels increased 1.3 and 1.5-fold over the controls in the presence of two abiotic elicitors (20 mumol/L CuSO4 and 100 mumol/L FeEDTA) and increased 1.8 and 1.6-fold in the presence of two biotic elicitors (0.5 mg/L beta-glucan and 0.5 mg/L chitosan). Maximum betacyanin synthesis with the two most effective elicitors was obtained when cultures were treated on day 1 and day 0 by beta-glucan and FeEDTA, respectively. A concentration-dependent response was exhibited by cultures treated with exogenous methyl jasmonate (MJ). MJ alone at 0.1 mumol/L caused a 2.6-fold increase in betacyanin synthesis when administered to the suspension culture on day 3. However, no additive effect on betacyanin accumulation was observed in treatments, which combined MJ and beta-glucan or FeEDTA. Treatment with ibuprofen (IB), an inhibitor of jasmonate biosynthesis, reduced the level of betacyanin in cells cultured in standard medium at all concentrations tested (25, 50, 100 mumol/L). The effect of IB on betacyanin synthesis in the cells treated with MJ or beta-glucan, however, differed with the IB concentration applied. The two higher concentrations (50 and 100 mumol/L) of IB significantly reduced the betacyanin content while the lower concentration (25 mumol/L) did not show an adverse effect on the betacyanin enhancement triggered by MJ or beta-glucan. Our findings suggest that, in suspension-cultured cells of Portulaca, an MJ-mediated signal transduction pathway prominently exists in betacyanin synthesis. This pathway seems to act antagonistically towards beta-glucan-mediated signaling. As far as we know this is the first report on the elevation of betacyanin level by jasmonate or other elicitors in cell suspension cultures.
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
Nazmul H
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Bhuiyan
Taiji
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Adachi
D000085:Acetates / Q000494:pharmacology*; D050858:Betalains; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D019327:Copper Sulfate / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D005936:Glucans / Q000494:pharmacology; D007052:Ibuprofen / Q000494:pharmacology; D054883:Oxylipins; D010860:Pigments, Biological / Q000096:biosynthesis*; D031822:Portulaca / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds; D015398:Signal Transduction / Q000187:drug effects
D000085:Acetates; D003517:Cyclopentanes; D005290:Ferric Compounds; D005936:Glucans; D054883:Oxylipins; D010860:Pigments, Biological; D000644:Quaternary Ammonium Compounds; D002686:Chitin; D050858:Betalains; C072239:methyl jasmonate; D048271:Chitosan; D004492:Edetic Acid; C019179:Fe(III)-EDTA; D019327:Copper Sulfate; D007052:Ibuprofen