Detection of N-(3-oxohexanoyl)-L-homoserine lactone in mice infected with Yersinia enterocolitica serotype O8.
Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, D-80336 Munich, Germany. jacobi@m3401.mpk.med.uni-muenchen.de
Christoph A
CA
Jacobi
Alexandra
A
Bach
Leo
L
Eberl
Anette
A
Steidle
Jürgen
J
Heesemann
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping; D015009:Yersinia Infections / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D015107:4-Butyrolactone