Crystal structure of Salmonella typhimurium 2-methylisocitrate lyase (PrpB) and its complex with pyruvate and Mg(2+).
Propionate metabolism in Salmonella typhimurium occurs via 2-methylcitric acid cycle. The last step of this cycle, the cleavage of 2-methylisocitrate to succinate and pyruvate, is catalysed by 2-methylisocitrate lyase (PrpB). Here we report the X-ray crystal structure of the native and the pyruvate/Mg(2+) bound PrpB from S. typhimurium, determined at 2.1 and 2.3A, respectively. The structure closely resembles that of the Escherichia coli enzyme. Unlike the E. coli PrpB, Mg(2+) could not be located in the native Salmonella PrpB. Only in pyruvate bound PrpB structure, Mg(2+) was found coordinated with pyruvate. Binding of pyruvate to PrpB seems to induce movement of the Mg(2+) by 2.5A from its position found in E. coli native PrpB. In both the native enzyme and pyruvate/Mg(2+) bound forms, the active site loop is completely disordered. Examination of the pocket in which pyruvate and glyoxalate bind to 2-methylisocitrate lyase and isocitrate lyase, respectively, reveals plausible rationale for different substrate specificities of these two enzymes. Structural similarities in substrate and metal atom binding site as well as presence of similar residues in the active site suggest possible similarities in the reaction mechanism.
Simanshu
Dhirendra K
DK
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Satheshkumar
P S
PS
Savithri
H S
HS
Murthy
M R N
MR
D001665:Binding Sites; D019755:Carbon-Carbon Lyases / Q000737:chemistry* / Q000145:classification; D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D046911:Macromolecular Substances; D008274:Magnesium / Q000737:chemistry*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D019289:Pyruvic Acid / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000737:chemistry* / Q000201:enzymology; D013379:Substrate Specificity
D046911:Macromolecular Substances; D019289:Pyruvic Acid; D019755:Carbon-Carbon Lyases; C417277:methylisocitrate lyase; D008274:Magnesium