Expression of soma-restricted proteolipid/DM20 proteins in lymphoid cells.
A new family of the myelin proteolipid protein (PLP/DM20) gene products, srPLP/DM20, has been identified recently in thymus and brain. In the central nervous system, srPLP/DM20 products are not localized in the myelin membrane, unlike their classic PLP/DM20 counterparts. In the immune system, the classic PLP/DM20 products appear to be expressed predominantly in thymic cortical epithelium. In this study, we examined the cellular expression of sr-PLP/DM20 proteolipids in lymphoid tissues and cells by immunohistochemistry, FACS analysis and RT-PCR. We found that in contrast to the classic PLP/DM20 products, sr-proteins are mainly expressed in developing thymocytes in thymus and in T- and B-lymphocytes in spleen. These results are of importance in our further understanding, not only the different role of these new PLP gene products in central and peripheral tolerance, but also the function of such products in lymphocyte biology.
Feng
Ji-Ming
JM
Developmental Biology Group, Mental Retardation Research Center, Neuropsychiatric Institute, University of California, Los Angeles, Medical School, Room 47-448, 760 Westwood Plaza, Los Angeles, CA 90024, USA.
Fernandes
Augustine O
AO
Bongarzone
Ernesto R
ER
Campagnoni
Celia W
CW
Kampf
Kathy
K
Campagnoni
Anthony T
AT
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D005434:Flow Cytometry; D007150:Immunohistochemistry; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins; D009928:Organ Specificity / Q000276:immunology; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000378:metabolism; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C047516:Plp1 protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger