Differentiation of Enterococcus faecium from Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains by PCR and dot-blot hybridisation.
Variations in length and sequence of the 16S/23S spacer region of Enterococcus faecium provided the basis for development of simple PCR and dot-blot hybridisation assays that enabled the differentiation of potentially probiotic Enterococcus faecium strains from Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Such assays may be useful for differentiation of yoghurt starter cultures and enterococcal strains when they are simultaneously present in probiotic food products.
Departamento de Nutrición y Bromatología III, Universidad Complutense de Madrid, 28040 Madrid, Spain.
S
S
Langa
A
A
Fernández
R
R
Martín
C
C
Reviriego
M L
ML
Marín
L
L
Fernández
J M
JM
Rodríguez
D000328:Adult; D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D016984:Enterococcus faecium / Q000145:classification* / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007223:Infant; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D008461:Meat Products / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D016415:Sequence Alignment; D013291:Streptococcus / Q000145:classification* / Q000235:genetics; D015014:Yogurt / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S