Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803.
The iron deficiency-dependent regulation of isiAB transcription in Synechocystis sp. PCC 6803 was analyzed by fusion of modified isiAB promoter fragments to gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic fur mutant a corresponding incomplete repression of isiAB transcription under iron-replete conditions confirmed the role of Fur in isiAB regulation. However, a 90 bp region upstream of the putative -35 box of the isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism.
Kunert
Anja
A
Fachbereich Biowissenschaften, Universität Rostock, Einsteinstr. 3a, D-18051, Rostock, Germany.
Vinnemeier
Josef
J
Erdmann
Norbert
N
Hagemann
Martin
M
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005418:Flavodoxin / Q000096:biosynthesis / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D007501:Iron / Q000494:pharmacology; D045342:Light-Harvesting Protein Complexes / Q000096:biosynthesis / Q000235:genetics; D009876:Operon / Q000187:drug effects / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D005418:Flavodoxin; D045342:Light-Harvesting Protein Complexes; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C076726:chlorophyll A binding protein CP43, Cyanobacteria; D007501:Iron