Changes in chloride homeostasis-regulating gene expressions in the rat hippocampus following amygdala kindling.
In a rat kindling model, we examined expression patterns of NKCC1, KCC1, KCC2, and CLC-2. In the dentate granule cell layer, there was an activity-dependent increase in NKCC1 mRNA but significant decreases in KCC1 and CLC-2 mRNAs. In addition, CLC-2 mRNA expression was markedly decreased in CA1 pyramidal layer. These results suggest that an increase in [Cl-]i and a resultant reduction in GABAergic inhibition may occur in hippocampus of epileptic rats.
Department of Physiology, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan. okabe@hama-med.ac.jp
Akihito
A
Okabe
Masamichi
M
Yokokura
Hiroki
H
Toyoda
Chigusa
C
Shimizu-Okabe
Koji
K
Ohno
Kohji
K
Sato
Atsuo
A
Fukuda
D000679:Amygdala / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels / Q000096:biosynthesis / Q000235:genetics; D002712:Chlorides / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D006706:Homeostasis / Q000235:genetics*; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D017966:Pyramidal Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D028021:Sodium-Potassium-Chloride Symporters / Q000096:biosynthesis / Q000235:genetics; D064506:Solute Carrier Family 12, Member 2; D027981:Symporters / Q000096:biosynthesis / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000502:physiology; D000096922:K Cl- Cotransporters
D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; D002712:Chlorides; D012333:RNA, Messenger; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2; D027981:Symporters; D005680:gamma-Aminobutyric Acid; D000096922:K Cl- Cotransporters; C578879:Slc12a2 protein, rat