Hydrogen bonds lengths in nucleic acids estimated from longitudinal nitrogen-15 relaxation.
A new NMR method has been designed for the measurement of the longitudinal relaxation rates of both donor and acceptor nitrogen-15 nuclei in Watson-Crick base pairs in 15N-enriched nucleic acids. The experiment was applied to a 22-nucleotide RNA hairpin. The lengths of four hydrogen bonds could be estimated from the longitudinal relaxation rates.
Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland.
Dimitri
D
Bytchenkoff
Geoffrey
G
Bodenhausen
D000465:Algorithms; D020029:Base Pairing; D003461:Crystallography / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry; D011119:Polynucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
D009587:Nitrogen Isotopes; D009696:Nucleic Acids; D011119:Polynucleotides; D012313:RNA; D006859:Hydrogen