[Molecular genetic methods in anaplastic large cell lymphoma].
BACKGROUND
Anaplastic large cell lymphoma belongs to a relatively good prognostic group of high grade non-Hodgkin lymphomas. Molecular genetic methods can provide great help in the differential diagnosis of the disease.


OBJECTIVE
Sixteen frozen lymph node samples of CD30 positive lymphomas with anaplastic morphology and 1 bone marrow aspiration were analysed with polymerase chain reaction according to T-cell receptor gene and immunglobulin heavy chain gene rearrangement. T(2;5)(p23;q35), t(1;2)(q25;p23) chromosomal translocation was also investigated with reverse transcriptase polymerase chain reaction.


RESULTS
Seven cases showed null-, 3 T-, 4 B- and 2 T/B hibrid genotype. Two cases of t(2;5), whereas no t(1;2) translocation was demonstrated.


CONCLUSIONS
Molecular genetic results correlated with the results of immunhistochemistry and can give further help to divide the cases into smaller, new prognostic subgroups.
Pécsi Tudományegyetem, Altalános Orvostudományi Kar, I. Belgyógyászati Klinika, Pécs. aszomor@clinics.pote.hu
Arpád
A
Szomor
Daniel
D
Roda
Prune
P
Zenou
Talal
T
al Saati
György
G
Csanaky
László
L
Pajor
Gábor
G
Kelényi
Georges
G
Delsol
Hajna
H
Losonczy
D000328:Adult; D000368:Aged; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000032:analysis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D007143:Immunoglobulin Heavy Chains; D017730:Ki-1 Antigen