Genetic transformation of Monascus purpureus DSM1379.
Monascus purpureus was transformed into hygromycin B resistance with hygromycin B phosphotransferase (hph) fused to Aspergillus nidulans trpC or a putative Monascus purpureus gpd1 promoter by electroporation. Among five strains, only M. purpureus DSM1397 was a competent recipient. Normal growth and sporulation on media containing up to 500 mg hygromycin B l(-1) occurred up to five generations. Upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hph as a selection marker, characteristic green fluorescence was observed under fluoromicroscopy indicating successful transformation.
Bioinformatics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Jeong-Gu
JG
Kim
Yang Do
YD
Choi
Yung-Jin
YJ
Chang
Soo-Un
SU
Kim
D025141:Drug Resistance, Fungal / Q000235:genetics*; D018274:Electroporation / Q000379:methods; D018014:Gene Transfer Techniques; D024861:Genetic Enhancement / Q000379:methods*; D006921:Hygromycin B / Q000494:pharmacology*; D032741:Monascus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase