Presence of the di-leucine motif in the cytoplasmic tail of the pig FcRn alpha chain.
The sequence of the pig FcRn alpha chain was recently published. The lack of a conserved di-leucine motif in the cytoplasmic tail suggests a rare polymorphism in the described animal, alternatively, a sequencing error. We therefore cloned and sequenced the pig FcRn alpha chain. Our sequence, along with a previous NCBI GenBank submission and five pig derived EST clones clearly demonstrate the presence of di-leucine motif in the cytoplasmic tail of the pig FcRn. No polymorphism in the cytoplasmic tail-encoding region was found in 25 animals from six pig breeds based on single-stranded conformation polymorphism and sequencing analysis, suggesting that the previously described pig FcRn alpha chain may represent a sequencing error in the 3' portion of the gene.
Department of Biosciences at Novum, Center for Biotechnology, Karolinska Institute, S-141 57 Huddinge, Sweden.
Yaofeng
Y
Zhao
Imre
I
Kacskovics
Zhihui
Z
Zhao
Lennart
L
Hammarström
D020816:Amino Acid Motifs / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D007930:Leucine / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011961:Receptors, Fc / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D011961:Receptors, Fc; D012313:RNA; D007930:Leucine