Peripheral sequences of the Serratia entomophila pADAP virulence-associated region.
Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-associated plasmid (pADAP). Herein, we report the DNA sequencing of approximately 50 kb upstream and 10 kb downstream of the virulence-encoding region. Based on similarity with proteins in the current databases, and potential ribosome-binding sites, 63 potential ORFs were determined. Eleven of these ORFs belong to a type IV pilus cluster (pilL-V) and a further eight have similarities to the translated products of the plasmid transfer traH-N genes of the plasmid R64. In addition, a degenerate 785-nt direct repeat flanks a 44.7-kb region with the potential to encode three Bacillus subtilis Yee-type proteins, a fimbrial gene cluster, the sep virulence-associated genes and several remnant IS elements.
Hurst
Mark R H
MR
Biocontrol and Biosecurity, AgResearch, PO Box 60, Lincoln, New Zealand. mark.hurst@agresearch.co.nz
O'Callaghan
Maureen
M
Glare
Travis R
TR
D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology; D023061:Gene Order; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D045522:Recombinases / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012705:Serratia / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D021901:DNA, Intergenic; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D045522:Recombinases; D012097:Repressor Proteins; C110533:SbmC protein, E coli; C109405:TapA protein, Pseudomonas; C065598:TraH protein, Bacteria