Determination of tiropramide in human plasma by liquid chromatography-tandem mass spectrometry.
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for the determination of tiropramide in human plasma was developed. Tiropramide and internal standard, cisapride were extracted from human plasma by liquid-liquid extraction and analyzed on a Luna C8 column with the mobile phase of acetonitrile-ammonium formate (10mM, pH 4.5) (50:50, v/v). The analytes was detected using an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curve was linear (r=0.998) over the concentration range of 2.0-200 ng/ml. The intra- and inter-assay coefficients of variation ranged from 2.8 to 7.8 and 6.7 to 8.9%, respectively. The recoveries of tiropramide ranged from 50.2 to 53.1%, with that of cisapride (internal standard) being 60.9+/-5.3%. The lower limit of quantification for tiropramide was 2.0 ng/ml using 100 microl plasma sample. This method was applied to the pharmacokinetic study of tiropramide in human.
Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.
Hye Won
HW
Lee
Hye Young
HY
Ji
Hee Hyun
HH
Kim
Hea-Young
HY
Cho
Yong-Bok
YB
Lee
Hye Suk
HS
Lee
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D010276:Parasympatholytics / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
D010276:Parasympatholytics; D014443:Tyrosine; C031173:tiropramide