Single chain antibody vaccination in mice against human ovarian cancer enhanced by microspheres and cytokines.
Experimental studies using monoclonal antibody have demonstrated that anti-idiotypic immunity can be enhanced by microspheres or cytokines. The underlying physiological principles behind these strategies involve sustained release of antigen and arousal of the immune system. In this study, a murine model was used to investigate the combination effect of microspheres and cytokines on anti-idiotypic response. A single chain antibody scFv-pDL1.0, which recognizes the human ovarian cancer antigen CA125 was formulated in microspheres and injected to mice alone or in the presence of colony stimulating factor (GM-CSF) or tumor necrosis factor (TNF-alpha). The immunization of mice with formulated single chain antibody and cytokines resulted in enhanced production of anti-idiotypic antibody, and which subsequently induced the production of anti-anti-idiotypic antibody. These results raise the possibility of cancer immunotherapy by administration of single chain antibody encapsulated in microspheres with GM-CSF or TNF-alpha.
State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, PUMC, Panjiayuan, Beijing, People's Republic of China. jiema2000@yahoo.com
Jie
J
Ma
Zheng
Z
Li
Dong
D
Luo
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D000917:Antibody Formation; D001483:Base Sequence; D018394:CA-125 Antigen / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007135:Immunoglobulin Variable Region; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D018394:CA-125 Antigen; D019496:Cancer Vaccines; D016207:Cytokines; D007135:Immunoglobulin Variable Region; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor