Geographic occurrence of intermediate type between Castanopsis sieboldii and C. cuspidata (Fagaceae) based on the structure of leaf epidermis.
The number of layers of epidermis in the leaves is used as a criterion to distinguish between Castanopsis sieboldii (two layers) and C. cuspidata (one layer). An intermediate type, which has one and two layers within a single leaf, is frequently seen in the field. The origin of the intermediate type has been supposed to be a hybrid between C. sieboldii and C. cuspidata. If the intermediate type is produced by hybridization, we expect that the F(1) seedlings of the intermediate type should occur in the co-occurrence area of those two species. To clarify the geographic occurrence of the intermediate type, we collected nuts of 443 mother trees from throughout the distribution area of the genus Castanopsis in Japan. A total of 7,260 seedlings germinated from these nuts were examined as to their leaf structure. The seedlings of the intermediate type occurred not only in the area where C. sieboldii and C. cuspidata coexisted, but also in the area where only C. sieboldii grows. The leaf structure of intermediate seedlings was independent of the traits of mother trees. These findings suggest that the intermediate seedlings that occurred in the area where only C. sieboldii grows are not hybrid between C. sieboldii and C. cuspidata but are a morphological variation of C. sieboldii. The difference in the structure of leaf epidermis is not always appropriate for the identification of the hybrid.
Forest Tree Breeding Center, 3809-1 Ishi-Kakozawa, Juo, Ibaraki 319-1301, Japan. hirooy@affrc.go.jp
Hiroo
H
Yamada
Tomiyasu
T
Miyaura
D029962:Fagaceae / Q000033:anatomy & histology / Q000145:classification* / Q000254:growth & development; D005843:Geography; D007564:Japan; D019684:Magnoliopsida / Q000033:anatomy & histology / Q000145:classification* / Q000254:growth & development; D018515:Plant Leaves / Q000033:anatomy & histology*; D013045:Species Specificity; D014197:Trees / Q000033:anatomy & histology / Q000254:growth & development