A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin.
The cytolethal distending toxin (CDT) of Haemophilus ducreyi is comprised of the CdtA, CdtB, and CdtC proteins, with the CdtB protein having demonstrated enzymatic (i.e., DNase) activity. Using a single recombinant Escherichia coli strain with two plasmids individually containing the H. ducreyi cdtA and cdtC genes, we purified a noncovalent CdtA-CdtC complex. Incubation of this CdtA-CdtC complex with HeLa cells blocked killing of these cells by CDT holotoxin. Furthermore, the addition of purified recombinant CdtB to HeLa cells preincubated with the CdtA-CdtC complex resulted in the killing of these human epithelial cells.
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9048, USA.
Kaiping
K
Deng
Eric J
EJ
Hansen
D001427:Bacterial Toxins / Q000032:analysis / Q000494:pharmacology* / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D006191:Haemophilus ducreyi / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology
D001427:Bacterial Toxins; C468508:CdtA protein, Haemophilus ducreyi; C468509:CdtC protein, Haemophilus ducreyi; D011994:Recombinant Proteins; C067044:cytolethal distending toxin