Detection of N-(3-oxohexanoyl)-L-homoserine lactone in mice infected with Yersinia enterocolitica serotype O8.
Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.
Jacobi
Christoph A
CA
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, D-80336 Munich, Germany. jacobi@m3401.mpk.med.uni-muenchen.de
Bach
Alexandra
A
Eberl
Leo
L
Steidle
Anette
A
Heesemann
Jürgen
J
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping; D015009:Yersinia Infections / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D015107:4-Butyrolactone