Spongy matrix of hyaluronic acid and collagen as a cultured dermal substitute: evaluation in an animal test.
The authors have developed a two-layered spongy matrix composed of hyaluronic acid (HA) and atelo-collagen (Col) as a cultured dermal substitute (CDS). This spongy matrix was applied to a full-thickness skin defect on the dorsum of Sprague-Dawley rats, and the wound conditions were observed over a period of 2 weeks. A piece of two-layered spongy matrix was applied to the wound, over which a commercially available polyurethane film dressing was applied. Both the macroscopic and the histological evaluations indicate that the two-layered spongy matrix is able to prepare a highly vascularized granulation tissue at an early stage. These findings suggest that this type of spongy matrix would be useful as a CDS.
Regenerative Tissue Engineering, Medical Sciences of Graduate School, Kitasato University, Kitasato 1-15-1, Sagamihara 228-8555, Japan.
Kentaro
K
Kubo
Yoshimitsu
Y
Kuroyanagi
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000494:pharmacology*; D020405:Dermis / Q000254:growth & development; D006097:Granulation Tissue / Q000098:blood supply / Q000254:growth & development; D006820:Hyaluronic Acid / Q000494:pharmacology*; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries*; D017768:Skin, Artificial; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000276:Adjuvants, Immunologic; D006820:Hyaluronic Acid; D003094:Collagen