Physical and functional interaction of the Werner syndrome protein with poly-ADP ribosyl transferase.
Werner's syndrome is a rare disease of premature ageing. The WRN gene product defective in this disorder belongs to the RecQ helicase family and is thought to be involved in DNA metabolism. Another protein, which plays an important role in both DNA replication and repair, is the poly-ADP ribosyl transferase. Here we demonstrate an interaction of these two proteins resulting in ADP-ribosylation of the WRN protein. These results imply that WRN is involved in DNA replication and in DNA repair.
Adelfalk
Caroline
C
Free University of Berlin, Department of Biochemistry, Thielallee 63, D-14195 Berlin, Germany. adelfalk@molgen.mpg.de
Kontou
Maria
M
Hirsch-Kauffmann
Monica
M
Schweiger
Manfred
M
D015153:Blotting, Western; D002460:Cell Line; D004265:DNA Helicases / Q000378:metabolism* / Q000502:physiology; D004260:DNA Repair; D004261:DNA Replication; D005090:Exodeoxyribonucleases; D006031:Glycosylation; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D053484:RecQ Helicases; D014898:Werner Syndrome / Q000201:enzymology / Q000473:pathology; D000071657:Werner Syndrome Helicase
D011065:Poly(ADP-ribose) Polymerases; D005090:Exodeoxyribonucleases; D004265:DNA Helicases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase