Dose-dependent protection by tomato against 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice.
This study was designed to investigate the protective role of pretreatment with graded doses of freshly prepared tomato paste against 7,12-dimethylbenz[a]anthracene (DMBA)-induced genetic damage and oxidative stress in male Swiss mice. The incidence of bone marrow micronuclei and the extent of hepatic lipid peroxidation and the antioxidants glutathione, glutathione peroxidase, and glutathione S-transferase were monitored. Three different concentrations (0.5, 1, and 2 g/kg body weight) of tomato paste were tested for their anticlastogenic effects against DMBA (35 mg/kg body weight). Increased frequency of micronuclei and enhanced lipid peroxidation accompanied by compromised antioxidant defenses were observed in DMBA-treated animals. Pretreatment with all three doses of tomato paste significantly reduced the frequencies of DMBA-induced micronuclei and oxidative stress. These findings demonstrate that administration of tomato paste protects against the clastogenic effects of DMBA by decreasing lipid peroxidation and enhancing the antioxidant status.
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
K V P
KV
Chandra Mohan
V
V
Bhuvaneswari
S K
SK
Abraham
S
S
Nagini
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002273:Carcinogens; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000737:chemistry*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation
D002273:Carcinogens; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione