Analysis of nutrient-dependent transcript variations in Neurospora crassa.
Nutrient-dependent variations in transcript levels of the filamentous fungus Neurospora crassa were studied on a microarray containing some 4700 cDNAs. Cells were grown in minimal and acetate medium. The isolated RNA was analyzed in comparison to the results obtained upon the hybridization of samples prepared from the RNA of cells grown in full medium. Altogether, 160 cDNA clones exhibited significant variations, falling into five distinct subgroups of very similar transcription profiles. This is indicative of the occurrence of a high degree of co-regulation of genes in N. crassa. Especially the regulation of the expression of proteins involved in metabolic pathways was found to be strongly regulated at the RNA level.
Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, D-69120, Heidelberg, Germany. v.aign@dkfz.de
Verena
V
Aign
Jörg D
JD
Hoheisel
D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000235:genetics*
D003470:Culture Media; D005656:Fungal Proteins; D012331:RNA, Fungal