Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors.
This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERalpha, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, alpha- and beta-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERalpha, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.
Département des Sciences des Denrées Alimentaires, Université de Liège, Bâtiment B-43bis, Sart Tilman, 4000 Liège, Belgium. mlscippo@ulg.ac.be
Marie-Louise
ML
Scippo
Catherine
C
Argiris
Cécile
C
Van De Weerdt
Marc
M
Muller
Philippe
P
Willemsen
Joseph
J
Martial
Guy
G
Maghuin-Rogister
D004702:Endocrine Glands / Q000187:drug effects*; D006801:Humans; D011944:Receptors, Androgen / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000737:chemistry*; D011980:Receptors, Progesterone / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D015262:Xenobiotics / Q000032:analysis* / Q000494:pharmacology
D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011994:Recombinant Proteins; D015262:Xenobiotics