Modeling the role of myosin 1c in neuronal growth cone turning.
We addressed the mechanical basis for how embryonic chick dorsal root ganglion growth cones turn on a uniform substrate of laminin-1. Turning is significantly correlated with lamellipodial area but not with filopodial length. We assessed the lamellipodial contribution to turning by asymmetric micro-CALI of myosin isoforms that causes localized lamellipodial expansion (myosin 1c) or filopodial retraction (myosin V). Episodes of asymmetric micro-CALI of myosin 1c (or myosin 1c and V together) caused significant turning of the growth cone. In contrast, repeated micro-CALI of myosin V or irradiation without added antibody did not turn growth cones. These findings argue that lamellipodia and not filopodia are necessary for growth cone turning. To model the role of myosin 1c on growth cone turning, we fitted the measured trajectories from asymmetric micro-CALI of myosin 1c-treated and untreated growth cones to the persistent random walk model. The first parameter in this equation, root-mean-square speed, is indistinguishable between the two data sets whereas the second parameter, the persistence of motion, is significantly increased (2.5-fold) as a result of asymmetric inactivation of myosin 1c by micro-CALI. This analysis demonstrates that growth cone turning results from an increase in the persistence of directional motion rather than a change in speed. Taken together, our results suggest that myosin 1c is a molecular correlate for directional persistence underlying growth cone motility.
Wang
Feng-Song
FS
Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
Liu
Can-Wen
CW
Diefenbach
Thomas J
TJ
Jay
Daniel G
DG
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003198:Computer Simulation; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D020439:Growth Cones / Q000502:physiology* / Q000648:ultrastructure*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D008959:Models, Neurological; D015233:Models, Statistical; D020409:Molecular Motor Proteins / Q000502:physiology*; D009068:Movement / Q000502:physiology*; D024461:Myosin Type I; D024701:Myosin Type V / Q000502:physiology; D009218:Myosins / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*
D020409:Molecular Motor Proteins; D024461:Myosin Type I; D024701:Myosin Type V; C460367:MYO1C protein, human; D009218:Myosins