Antioxidant principles from the needles of red pine, Pinus densi fl ora.
Antioxidant activity of Pinus densiflora Sieb. et Zucc. (Pinaceae) was evaluated for potential to inhibit hydroxyl radicals, inhibit total reactive oxygen species generation in kidney homogenates using 2',7'-dichlorodihydro fluorescein diacetate (DCHF-DA) and scavenge authentic peroxynitrites. The methanolic extract of P. densiflora showed strong antioxidant activity in the tested model systems and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate > n-butanol > water > dichloromethane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further puri fi ed by repeated silica gel and Sephadex LH-20 column chromatographies. An active lignan (+)-isolarisiresinol xylopyranoside, as well as two active flavonoids [kaempferol 3-O-beta-galactopyranoside and its 6"-acetyl derivative], were isolated.
Jung
Mee Jung
MJ
Faculty of Food Science and Biotechnology, Pukyong National University, Pusan, Korea.
Chung
Hae Young
HY
Choi
Jin Ho
JH
Choi
Jae Sue
JS
D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D017665:Hydroxyl Radical; D007668:Kidney / Q000187:drug effects; D017705:Lignans / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D030421:Peroxynitrous Acid; D008517:Phytotherapy; D010851:Picrates; D028223:Pinus; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018514:Plant Structures; D017382:Reactive Oxygen Species
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D017705:Lignans; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D030421:Peroxynitrous Acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl