Reduced nonspecific adsorption on covalently immobilized protein surfaces using poly(ethylene oxide) containing blocking agents.
In a number of applications, e.g. DNA/protein micro-array technology, enzyme-linked immunosorbent assay (ELISA) technology or surface plasmon resonance (SPR) technology, the covalent coupling of proteins to surfaces is required. Following the covalent coupling of proteins, the remaining reactive groups should be blocked in order to avoid covalent binding of the analyte to the reactive surface. To this end, preferably blocking agents containing groups that avoid nonspecific adsorption should be used. These blocking agents are typically ethanolamine and cysteine for protein coupling via amino groups and thiol groups, respectively. This report presents novel blocking agents containing poly(ethylene oxide) (PEO) groups. These blocking agents show enhanced qualities to avoid nonspecific adsorption and can therefore have advantages in versatile protein-surface technologies.
IMEC, MCP-BIO, Kapeldreef 75, B-3001 Louvain, Belgium. Frederix@imec.be
Filip
F
Frederix
Kristien
K
Bonroy
Gunter
G
Reekmans
Wim
W
Laureyn
Andrew
A
Campitelli
Mikhael A
MA
Abramov
Wim
W
Dehaen
Guido
G
Maes
D000327:Adsorption; D001671:Biochemistry / Q000379:methods*; D006046:Gold; D007074:Immunoglobulin G / Q000378:metabolism; D010169:Palmitic Acids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014168:Transferrin / Q000378:metabolism
C450319:16-mercaptohexadecanoic acid; D007074:Immunoglobulin G; D010169:Palmitic Acids; D011506:Proteins; D014168:Transferrin; D011092:Polyethylene Glycols; D006046:Gold