Glycosynthase-catalysed syntheses at pH below neutrality.
The use of the new glycosynthase Ta-beta-GlyE386G and of the already known Ss-beta-GlyE387G for the synthesis of interesting 4-methylumbelliferyl disaccharides and for the galactosylation of alpha- and beta-xylosides of 4-penten-1-ol is reported. The results show satisfactory yields of reaction in presence of low excesses of acceptors and demonstrated that the high activity of these enzymes at pH below neutrality is applicable in the transfer of glucose as well as of galactose from the preferred 2-NP-based donors.
Trincone
Antonio
A
Istituto di Chimica Biomolecolare C.N.R., Via Campi Flegrei 34, 80072 Pozzuoli, Naples, Italy. atrincone@icmib.na.cnr.it
Giordano
Assunta
A
Perugino
Giuseppe
G
Rossi
Mosè
M
Moracci
Marco
M
D019943:Amino Acid Substitution; D004187:Disaccharides / Q000138:chemical synthesis; D006006:Glycogen Synthase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013379:Substrate Specificity
D004187:Disaccharides; D006006:Glycogen Synthase