Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics.
Carbohydrate microarray technologies are new developments at the frontiers of glycomics. Results of 'proof of concept' experiments with carbohydrate-binding proteins of the immune system - antibodies, selectins, a cytokine and a chemokine - and several plant lectins indicate that microarrays of carbohydrates (glycoconjugates, oligosaccharides and monosaccharides) will greatly facilitate not only surveys of proteins for carbohydrate-binding activities but also elucidation of their ligands. It is predicted that both naturally occurring and synthetic carbohydrates will be required for the fabrication of microarrays that are sufficiently comprehensive and representative of entire glycomes. New leads to biological pathways that involve carbohydrate-protein interactions and new therapeutic targets are among biomedically important outcomes anticipated from applications of carbohydrate microarrays.
Feizi
Ten
T
The MRC Glycosciences Laboratory, Imperial College London, Northwick Park & St Mark's Hospital Campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK. t.feizi@imperial.ac.uk
Fazio
Fabio
F
Chai
Wengang
W
Wong
Chi Huey
CH
D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015336:Molecular Probe Techniques / Q000295:instrumentation* / Q000639:trends*; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry
D002241:Carbohydrates; D015335:Molecular Probes; D009844:Oligosaccharides