Augmented PLA2 activity in pre-eclamptic decidual tissue--a key player in the pathophysiology of 'acute atherosis' in pre-eclampsia?
Decidual acute atherosis is associated with pre-eclampsia, but the underlying mechanism is still unclear. We have previously demonstrated elevated level of the oxidative stress marker 8-isoprostaglandin F(2 alpha)(8-isoprostane) and lipids in pre-eclamptic decidual tissue. Arachidonic acid (AA) in tissue phospholipids is a source for 8-isoprostane generation, and 8-isoprostane is liberated from tissue phospholipids by phospholipase A(2)(PLA(2)). The aims of this study were to explore whether AA content or PLA(2)expression in pre-eclamptic decidual tissue differed from controls. Decidua basalis tissues were obtained by vacuum aspiration at Caesarean delivery in pre-eclamptic and control pregnancies. We demonstrated a statistically significantly higher total PLA(2)activity in pre-eclamptic decidua compared to control tissue. On the other hand, no differences in AA content of tissue phospholipids or protein expression of secretory and cytosolic PLA(2)between pre-eclamptic and control decidual tissue were found. In conclusion, the elevated level of free 8-isoprostane in pre-eclamptic decidual tissue could be caused by augmented PLA(2)activity. We speculate that an elevated PLA(2)enzyme activity in pre-eclamptic decidual tissue could be of importance in the pathogenesis of 'acute atherosis', comparable to the atherogenesis in cardiovascular diseases.
Department of Obstetrics and Gynecology, Ulleval University Hospital, Kirkeveien 166, 0450 Oslo, Norway. annetine.staff@uleval.no
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D001055:Apolipoproteins B / Q000032:analysis; D016718:Arachidonic Acid / Q000032:analysis; D001161:Arteriosclerosis / Q000209:etiology; D015992:Body Mass Index; D003600:Cytosol / Q000737:chemistry; D033301:Deciduoma / Q000737:chemistry / Q000201:enzymology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011225:Pre-Eclampsia / Q000150:complications / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D001055:Apolipoproteins B; D005227:Fatty Acids; D012333:RNA, Messenger; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2