Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice.
We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001).
Virology Department, PAHO/WHO Collaborating Center for Viral Diseases, Pedro Kourí Tropical Medicine Institute, Havana, Cuba. mayra@ipk.sld.cu
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M
Muné
R
R
Rodríguez
R
R
Ramírez
Y
Y
Soto
B
B
Sierra
R
R
Rodríguez Roche
G
G
Marquez
J
J
Garcia
G
G
Guillén
M G
MG
Guzmán
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003715:Dengue / Q000517:prevention & control; D003716:Dengue Virus / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines