Mice bearing Acads mutation display altered postingestive but not 5-s orosensory response to dietary fat.
A previous survey of mouse inbred strains revealed a wide range in self-selected fat intake, from 26 to 83% of energy. The BALB/cByJ strain selected a lower percentage of fat intake (36%) than all other strains tested except for the CAST/Ei. BALB/cByJ mice are deficient in the short-chain acyl-CoA dehydrogenase (SCAD) enzyme due to a spontaneous mutation in Acads. We hypothesized that this deficiency would alter fat appetite and used three behavioral test paradigms to compare the response of BALB/cByKz. Acads -/- and BALB/cByKz. Acads +/+ mice to fat stimuli. First, during 10-day exposure to a macronutrient self-selection diet, Acads -/- mice consumed proportionately less fat and more carbohydrate than Acads +/+ mice, yet total energy intake was similar between strains. Next, in 48-h two-bottle preference tests, Acads +/+ mice displayed a preference for 50% corn oil, but Acads -/- mice did not. Finally, in brief-access taste tests employing successive 5-s presentations of corn oil in an ascending concentration series ending with 50%, there were no effects of strain on total licks, indicating that Acads does not alter acute orosensory response to this fat stimulus. With 15-s presentations, however, the Acads +/+ mice licked more of the 50% oil than Acads -/-, suggesting orosensory effects related to the increased exposure time. In contrast to corn oil, there were no strain differences in licking response to sucrose solution in either the two-bottle or brief-access taste tests. The observation that SCAD-deficient mice display altered postingestive responses to dietary fat provides further evidence for the metabolic control of feeding.
Pennington Biomedical Research Center, 6400 Perkins Rd., Baton Rouge, LA 70808-4124, USA. richarbk@pbrc.edu
Brenda K
BK
Smith Richards
Brenda N
BN
Belton
Barbara
B
York
Julia
J
Volaufova
D000818:Animals; D044926:Butyryl-CoA Dehydrogenase / Q000172:deficiency / Q000235:genetics*; D003314:Corn Oil; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D004327:Drinking Behavior; D005247:Feeding Behavior; D005518:Food Preferences; D017353:Gene Deletion; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D009055:Mouth / Q000187:drug effects* / Q000502:physiology*; D009154:Mutation / Q000502:physiology; D009994:Osmolar Concentration; D012677:Sensation; D013395:Sucrose; D013997:Time Factors
D004041:Dietary Fats; D013395:Sucrose; D003314:Corn Oil; D044926:Butyryl-CoA Dehydrogenase