[Real-time fluorescent PCR for screening AZFc/DAZ microdeletions on the Y chromosome in male infertility patients].
OBJECTIVE
To develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.


METHODS
A set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome.


RESULTS
Eleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients.


CONCLUSIONS
The real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR.
Reproductive Medical Center, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, China. yucongyi01@sohu.com
Cong-Yi
CY
Yu
Guang-Lun
GL
Zhuang
Can-Quan
CQ
Zhou
Zong-He
ZH
Yan
Wei
W
Li
Hua
H
Gao
De-Rong
DR
Rui
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D000075364:Deleted in Azoospermia 1 Protein; D005453:Fluorescence; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics*
C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins