Analysis of fenhexamid in caneberry, blueberry, and pomegranate by liquid chromatography-tandem mass spectrometry.
An analytical method for the determination of fenhexamid [N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide] in caneberry, blueberry, and pomegranate was developed utilizing acetone extraction, column cleanup, liquid-liquid partitioning, and liquid chromatography-tandem mass spectroscopy (LC-MS/MS) for detection. Method validation recoveries ranged from 91 to 96% for caneberry, from 80 to 91% for blueberry, and from 74 to 95% for pomegranate. Control samples collected from IR-4 trials for all matrixes had residue levels of <0.020 ppm. Fenhexamid-treated field samples had residue levels that ranged from 0.46 to 16.11 ppm (caneberry), from 0.87 to 2.91 ppm (blueberry), and from 1.59 to 1.85 ppm (pomegranate). The method was validated to a limit of quantitation of 0.020 ppm, and the limit of detection was 0.009 ppm.
Department of Environmental Toxicology, University of California at Davis, One Shields Avenue, Davis, California 95616, USA. mjhengel@ucdavis.edu
Matt
M
Hengel
B
B
Hung
Jo
J
Engebretson
Takayuki
T
Shibamoto
D000577:Amides / Q000032:analysis*; D029798:Blueberry Plants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D029561:Lythraceae / Q000737:chemistry*; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis; D011786:Quality Control
D000577:Amides; D005659:Fungicides, Industrial; D010573:Pesticide Residues; C451426:N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide