In vitro infection of human primary adipose cells with HIV-1: a reassessment.
We reassessed the infection ability of human primary preadipocytes. The use of X4, R5 or VSV-G-pseudotyped viral particles indicated that viral entry is the limiting step. However, transfection with HIV-1 receptors restored efficient infection. Analyses of CD4, CXCR4 and CCR5 expression on preadipocytes and adipocytes revealed that receptor co-expression levels did not permit HIV-1 entry into adipose cells from all biopsies tested. We concluded that adipose tissue cannot be infected with HIV-1 in vivo.
Institut Cochin, INSERM U567/CNRS UMR8104/Université Paris 5-René Descartes, Département des Maladies Infectieuses, 22 rue Méchain, 75014 Paris, France.
Sandie
S
Munier
Alejandra
A
Borjabad
Morgane
M
Lemaire
Virginie
V
Mariot
Uriel
U
Hazan
D017667:Adipocytes / Q000821:virology*; D000273:Adipose Tissue / Q000821:virology; D015704:CD4 Antigens / Q000235:genetics; D015870:Gene Expression; D015658:HIV Infections / Q000235:genetics / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D019713:Receptors, CCR5 / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics; D014162:Transfection
D015704:CD4 Antigens; D019713:Receptors, CCR5; D019718:Receptors, CXCR4