[Studies of TGF-beta/Smads expression in lung cancer].
Smad proteins transduce signals from transforming growth factor beta superfamily ligands that regulate cell proliferation, differentiation and death through activation of receptor serine/threonine kinases. TGF-beta/Smads signal pathway not only has transforming potential but can also drive tumourigenesis, malignant progression, invasion and metastasis of human cancers. Using the immuno-histochemistry, we investigate the expression and location of TGF-beta R II, Smad2, Smad4 and Smad7 in 20 lung cancer specimens and 8 lung cancer cell lines. The results suggest that aberrant smads protein expression is significantly related to lung cancer tumoruigenesis and progression. Interestingly, TGF-beta R II and Smad7 strongly express in high metastasis cell lines. High expression of TGF-beta R II and smad7 in the cell lines with high-metastatic potential showed a conceivable TGF-beta signal pathway independent Smads in the lung cancer, and that might mediate invasion and metastasis of lung cancer.
Shen
Jing-Ling
JL
Department of Medical Genetics, Harbin Medical University, Harbin 150086, China.
Yan
Cheng-Hui
CH
Liu
Yan
Y
Yan
Xing-Qi
XQ
Zhang
Xiao-Ling
XL
Jin
Yan
Y
Zhang
Ke-Feng
KF
Sang
Zhan-Fa
ZF
Zhang
Gui-Yin
GY
Li
Pu
P
Fu
Song-Bin
SB
D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis*; D051899:Smad2 Protein; D051901:Smad4 Protein; D051906:Smad7 Protein; D015534:Trans-Activators / Q000032:analysis*
D004268:DNA-Binding Proteins; D018125:Receptors, Transforming Growth Factor beta; C497782:SMAD2 protein, human; C497796:SMAD4 protein, human; C497806:SMAD7 protein, human; D051899:Smad2 Protein; D051901:Smad4 Protein; D051906:Smad7 Protein; D015534:Trans-Activators; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II