Cryopreservation of cord blood after liquid storage.
BACKGROUND
The processing of cord blood may result in delays prior to RBC depletion and cryopreservation. The overall objective of this investigation is to determine the influence of liquid storage prior to cryopreservation on the post-thaw viability.


METHODS
UC blood supplemented with CPD anticoagulant (CB) was obtained from normal donors with informed consent. CB was stored undiluted, or diluted with 1:1 ratio of storage solution STM-sav for up to 72 h. The undiluted control samples were stored at room temperature. CB samples supplemented with STM-sav were stored at 4 degrees C. After completion of the storage protocol, the sample was RBC depleted, frozen, stored, thawed, and assayed for viability. Nucleated cell counts, percentage of CD34+ cells, and frequency of colony formation were determined during liquid storage and after cryopreservation.


RESULTS
The post-thaw mononuclear cell recovery and viability of cord blood stored for 72 h was significantly lower than that of cord blood stored for 24 h prior to cryopreservation. This difference was true for cord bloods stored in STM-sav and controls. Dilution of the cord blood with STM-sav improved the frequency of CFU-GM observed.


CONCLUSIONS
Liquid storage of cord blood for 24 h prior to cryopreservation does not adversely influence the post-thaw cell recovery. The use of a storage solution (STM-Sav) enhances the retention of colony-forming capabilities post-thaw. These and other studies provide an important foundation for the development of integrated protocols for cord blood banking.
Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
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A
Hubel
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Carlquist
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Clay
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McCullough
D018952:Antigens, CD34 / Q000276:immunology; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D003080:Cold Temperature / Q000009:adverse effects; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000637:transplantation*; D006801:Humans; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000637:transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D018952:Antigens, CD34; D003451:Cryoprotective Agents; D017493:Leukocyte Common Antigens