Real-time multicolor DNA detection with chemoresponsive diffraction gratings and nanoparticle probes.
We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound nanoparticle probe based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protocol, displaying attributes of both detection methodologies: the high sensitivity and selectivity afforded by nanoparticle probes and the experimental simplicity, wavelength-dependent resonant enhancement features, and miniaturization potential provided by the diffraction-based sensing technology.
Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208, USA.
Ryan C
RC
Bailey
Jwa-Min
JM
Nam
Chad A
CA
Mirkin
Joseph T
JT
Hupp
D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000235:genetics; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D007834:Lasers; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010316:Particle Size; D013056:Spectrophotometry, Ultraviolet
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D015342:DNA Probes; D004277:DNA, Single-Stranded; C030325:anthrax toxin; D004247:DNA