Carnitine profiles during differentiation and effects of carnitine on differentiation of 3T3-L1 cells.
These experiments were conducted to investigate carnitine profiles during differentiation and the effects of carnitine on the differentiation of 3T3-L1 cells. To induce cell differentiation, undifferentiated 3T3-L1 cells were treated with dexamethasone, 1-methyl-3-isobutylxanthine, and d-biotin. Carnitine was also exogenously added to the cells to test its effect on cell differentiation. Triglyceride, total lipid, total protein, nonesterified carnitine, acid-soluble acylcarnitine, and acid-insoluble acylcarnitine were analyzed during the differentiation of 3T3-L1 cells. Total lipid, triglyceride, and total protein increased during the 3T3-L1 cell differentiation. However, nonesterified carnitine, acid-soluble acylcarnitine, and acid-insoluble acylcarnitine concentrations were lower in the differentiated 3T3-L1 cells. In addition, the exogenously added carnitine inhibited the increases in triglyceride and total lipid levels. These results suggest that carnitine may have an inhibitory role on the early stage of 3T3-L1 cell differentiation.
Department of Food Science and Human Nutrition and Institute for Molecular Biology and Genetics, Chonbuk National University, Korea. cha8@moak.chonbuk.ac.kr
Youn-Soo
YS
Cha
Jae-Soon
JS
Eun
Suk-Heung
SH
Oh
D041721:3T3-L1 Cells; D000818:Animals; D002331:Carnitine / Q000378:metabolism / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D050356:Lipid Metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D011506:Proteins; D014280:Triglycerides; D002331:Carnitine