Fluorescent complex of pyoverdin with aluminum.
When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.
Departamento de Nutrición y Bromatologi;a III, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040, Madrid, Spain.
A
A
del Olmo
C
C
Caramelo
C
C
SanJose
D000535:Aluminum / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D005453:Fluorescence; D009842:Oligopeptides; D010860:Pigments, Biological / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000737:chemistry; D017262:Siderophores / Q000737:chemistry
D005290:Ferric Compounds; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D000535:Aluminum