Liquid chromatography-electrospray mass spectrometry (LC-MS) method for determination of esmolol concentration in human plasma.
Esmolol is a very short-acting beta-blocker commonly used in critically ill patients. To study the pharmacokinetics of esmolol in pediatric patients, a liquid chromatography-electrospray mass spectrometry (LC-MS) method to quantitate esmolol concentration in plasma was developed. Following methylene chloride extraction from 200 microl aliquots of plasma containing internal standard and reconstitution in 0.05% formic acid, 10 microl are injected onto column, eluted using a methanol/formic acid gradient over 15 min, and monitored with selected ion recording at 296.2 and 282.2. The assay is linear between 2 and 1000 ng/ml. The intra-day and inter-day coefficients of variation are less than 8 and 10%, respectively.
Division of Clinical Pharmacology and Therapeutics, Department of Pediatrics, The Children's Hospital of Philadelphia, Abramson Research Center, Suite 907, 3615 Civic Center Blvd., Philadelphia, PA 19104-4318, USA.
Athena F
AF
Zuppa
Heng
H
Shi
Peter C
PC
Adamson
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002138:Calibration; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011412:Propanolamines / Q000097:blood*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C036604:esmolol