Sodium pyruvate protects against H(2)O(2) mediated apoptosis in human neuroblastoma cell line-SK-N-MC.
Free radicals are involved in neuronal damage. The present study was aimed to investigate the protective effect of sodium pyruvate-a free radical scavenger against hydrogen peroxide (H(2)O(2)) induced apoptosis in human neuroblastoma cell line-SK-N-MC. On exposure to H(2)O(2) (0.025 mM) cells exhibited apoptosis within 24 h, demonstrating a high caspase 3 activity by 3 h followed by cleavage of PARP that was maximum at 24 h. A break down in the mitochondrial membrane potential was observed 3 h onwards. Sodium pyruvate protected cells significantly (P<0.05) against apoptosis in a dose dependent manner as assessed for cell viability by dye exclusion method and apoptosis by TUNEL. Sodium pyruvate significantly inhibited caspase 3 activity, cleavage of PARP and breakdown of mitochondrial membrane potential. These data suggest that sodium pyruvate protects neuronal damage caused by H(2)O(2).
Jagtap
Jayashree C
JC
Department of Biotechnology, National Center for Cell Science, Government of India An Autonomous Institution, Pune University Campus, Ganeshkhind, 411007 Pune, India.
Chandele
Anmol
A
Chopde
B A
BA
Shastry
Padma
P
D000975:Antioxidants; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002470:Cell Survival; D003124:Colorimetry; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D008564:Membrane Potentials / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology*; D018696:Neuroprotective Agents; D016877:Oxidants / Q000633:toxicity*; D019289:Pyruvic Acid / Q000494:pharmacology*
D000975:Antioxidants; D018696:Neuroprotective Agents; D016877:Oxidants; D019289:Pyruvic Acid; D006861:Hydrogen Peroxide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases