Bovine herpesvirus 5 (BoHV-5) in bull semen: amplification and sequence analysis of the US4 gene.
Bovine herpesvirus type 5 (BoHV-5), which is potentially neuropathogenic, was detected in clinical samples of bovine semen, both directly and after isolation in cell culture, using a nested PCR system for amplifying the US4 gene. Nucleotide sequences generated from the amplicons were analysed and deposited at GenBank (NCBI, Bethesda, MD, USA) under the accession numbers AF298174 and AF330157. Alignment of these sequences and previously deposited sequences of BoHV-1 and BoHV-5 showed 82% and 98% similarity, respectively. The bulls, which were maintained at an artificial insemination centre, had presented no clinical signs, indicating that bovine semen should be screened for BoHV-5 to prevent transmission of the virus.
Laboratório de Biologia de Microorganisms Intracelulares, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, caixa postal 486, CEP 31270-901, Belo Horizonte, MG, Brazil.
L I
LI
Gomes
M A
MA
Rocha
J G
JG
Souza
E A
EA
Costa
E F
EF
Barbosa-Stancioli
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005784:Gene Amplification; D006566:Herpesviridae Infections / Q000235:genetics / Q000662:veterinary*; D025881:Herpesvirus 5, Bovine / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012661:Semen / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D000914:Antibodies, Viral; D004279:DNA, Viral; C102256:US4 protein, bovine herpesvirus 5; D014764:Viral Proteins