Identification of a metabolic network structure representative of Arthrospira (spirulina) platensis metabolism.
A comprehensive network structure for the autotrophic growth of Arthrospira platensis is proposed. The metabolic network was built up with 121 reactions and 134 metabolites including biomass synthesis, production of a growth-associated exopolysaccharide, and energy aspects. The model supports the existence of a metabolic shunt of PEP to pyruvate through PEP carboxylase, NAD(+)-dependent malate dehydrogenase and malic enzyme to convert NADH,H(+) into NADPH,H(+). A limit in Arthrospira growth metabolism due to NADH,H(+) balancing is evidenced, explaining why the maximal light-dependent mass yield of the growth-associated exopolysaccharide was 0.51 kg EPS kg(-1) biomass, consistent with experimental results.
Laboratoire de Génie Chimique et Biochimique, Université Blaise Pascal, CUST, 24 avenue des Landais, BP 206, 63174 Aubière-Cédex, France. Guillaume.Cogne@univ-bpclermont.fr
Guillaume
G
Cogne
J-B
JB
Gros
C-G
CG
Dussap
D002455:Cell Division / Q000502:physiology; D003198:Computer Simulation; D000458:Cyanobacteria / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism*; D018462:Photobiology / Q000379:methods; D011134:Polysaccharides / Q000096:biosynthesis*; D015398:Signal Transduction / Q000502:physiology*
D009097:Multienzyme Complexes; D011134:Polysaccharides