Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells.
From the fruiting bodies of the edible mushroom Lentinus edodes, a novel protein designated lentin with potent antifungal activity was isolated. Lentin was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and Mono S. The N-terminal sequence of lentin manifested similarity to endoglucanase. Lentin, which had a molecular mass of 27.5 kDa, inhibited mycelial growth in a variety of fungal species including Physalospora piricola, Botrytis cinerea and Mycosphaerella arachidicola. Lentin also exerted an inhibitory activity on HIV-1 reverse transcriptase and proliferation of leukemia cells.
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Patrick H K
PH
Ngai
T B
TB
Ng
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D005656:Fungal Proteins / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D007938:Leukemia / Q000188:drug therapy; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry*
D000935:Antifungal Agents; D005656:Fungal Proteins; D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; C478883:lentin protein, Lentinus edodes; D054303:HIV Reverse Transcriptase