Instantaneous, stoichiometric generation of powerfully reducing states of protein active sites using Eu(II) and polyaminocarboxylate ligands.
Addition of an equivalent of a polyaminocarboxylate ligand (L) to a solution of a redox protein and the aqua Eu2+ ion results in the instantaneous in situ generation of a very powerful reductant Eu(II)-L that can rapidly drive an electron stoichiometrically onto a redox centre having an extremely negative reduction potential (lower than -1 V): this is exemplified by straightforward generation of the super-reduced state of the Fe-protein of nitrogenase.
Inorganic Chemistry Laboratory, Oxford University, South Parks Road, Oxford, UK OX1 3QR.
Kylie A
KA
Vincent
Gareth J
GJ
Tilley
Nina C
NC
Quammie
Ian
I
Streeter
Barbara K
BK
Burgess
Myles R
MR
Cheesman
Fraser A
FA
Armstrong
D016948:Azotobacter vinelandii / Q000201:enzymology; D001665:Binding Sites; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000737:chemistry*; D004563:Electrochemistry; D005063:Europium / Q000737:chemistry*; D008024:Ligands; D009591:Nitrogenase / Q000737:chemistry*; D019159:Nonheme Iron Proteins / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D004369:Pentetic Acid / Q000031:analogs & derivatives / Q000737:chemistry*
D008024:Ligands; D019159:Nonheme Iron Proteins; D009942:Organometallic Compounds; D005063:Europium; D004533:Egtazic Acid; D004369:Pentetic Acid; D009591:Nitrogenase