Localization of cap-binding protein in subcellular fractions of HeLa cells.
The 26,000-M(r) cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.
Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah 84132, USA.
J L
JL
Hansen
D O
DO
Etchison
J W
JW
Hershey
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E
Ehrenfeld
D002458:Cell Fractionation; D006367:HeLa Cells; D006801:Humans; D017955:Poliovirus / Q000502:physiology; D039381:RNA Cap-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D012315:RNA Caps / Q000378:metabolism*
D039381:RNA Cap-Binding Proteins; D012315:RNA Caps