A comparative evaluation of accelerated solvent extraction and Polytron extraction for quantification of lipids and extractable organochlorine in fish.
Optimal conditions for extraction methods using an accelerated solvent extractor (ASE) and a Polytron homogenizer were established for quantification of (crude) lipids and extractable organochlorine (EOCl) in fish. The two methods, an ASE consecutive extraction at 55 and 100 degrees C for ground, freeze-dried filets, and a Polytron extraction at room temperature with four repetitions for ground, wet filets and with two repetitions for wet fish livers were evaluated in terms of reproducibility and comparability. With respect to lipid measurement, the two methods have comparable reproducibility and the experimental errors are relatively small compared to natural variations of fish to fish within a sampling site. For EOCl measurement, the relative standard deviation tends to increase with decreasing levels of EOCl in specimens. An important factor for the relative standard deviation of measurements of analytes present at trace levels in fish matrices is sampling error in taking aliquots. Statistical analysis using a paired comparison design showed that measurement values (crude lipids or EOCl) obtained from these two extraction methods are, in general, not equivalent. There is a good correlation between the two methods for lipid measurement; whereas for EOCl measurements, high degrees of correlation exist within the exposed fish group, but not in the reference group.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College St., Toronto, ON, Canada M5S 3E5. bzhuang@taro.ca
Wenshan
W
Zhuang
Bruce
B
McKague
Douglas
D
Reeve
John
J
Carey
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D005069:Evaluation Studies as Topic; D005399:Fishes; D006843:Hydrocarbons, Chlorinated / Q000302:isolation & purification*; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D012016:Reference Values; D015203:Reproducibility of Results
D006843:Hydrocarbons, Chlorinated; D008055:Lipids