The effect of alprostadil on ischemia-reperfusion injury of peripheral nerve in rats.
OBJECTIVE
To determine the effect of alprostadil on the ischemia-reperfusion (I/R) injury of the sciatic nerve in rats.


METHODS
Pre-reperfusion administration of alprostadil (0.05 microg kg(-1)) was assessed in the I/R injury model of the rat sciatic nerve. In this model, blood samples were investigated for the I/R injury markers namely malondialdehyde (MDA), an indicator of lipid peroxidation, and nitrite/nitrate levels, products of nitric oxide (NO) metabolism.


RESULTS
A significant decrease in MDA, and increase in NO levels were observed in the groups which received alprostadil before reperfusion, when compared to their corresponding untreated controls (I/R only) at all time intervals (P=0.0001). There was a statistically significant difference in both MDA, and NO levels between certain time intervals. There was no statistical linear correlation between MDA and NO levels.


CONCLUSIONS
Alprostadil may be suggested as a protective anti-inflammatory and a vasodilator pharmacological agent for I/R injury in peripheral nerves. Also, measurements of NO and MDA may be complementary to the generally accepted evaluation parameters of I/R injury including electromyography and nerve histopathology.
Department of Orthopaedics and Traumatology, Faculty of Medicine, University of Mersin, Ihsaniye Mah 4935 sok, No. 3, 33079 Mersin, Turkey. amilcan@mersin.edu.tr
Abtullah
A
Milcan
Emrah
E
Arslan
Ozlen Tubay
OT
Bagdatoglu
Celal
C
Bagdatoglu
Gurbuz
G
Polat
Arzu
A
Kanik
Derya Umit
DU
Talas
Fehmi
F
Kuyurtar
D000527:Alprostadil / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000293:injuries; D005268:Femoral Vein / Q000293:injuries; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000737:chemistry; D009480:Neuropharmacology / Q000379:methods; D009569:Nitric Oxide / Q000096:biosynthesis / Q000097:blood / Q000737:chemistry; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D012584:Sciatic Nerve / Q000098:blood supply / Q000187:drug effects* / Q000293:injuries; D013997:Time Factors
D009573:Nitrites; D009569:Nitric Oxide; D008315:Malondialdehyde; D000527:Alprostadil