Linoleic acid 10-hydroperoxide as an intermediate during formation of 1-octen-3-ol from linoleic acid in Lentinus decadetes.
In order to confirm the biosynthetic pathway to 1-octen-3-ol from linoleic acid, a crude enzyme solution was prepared from the edible mushroom, Lentinus decadetes. When the reaction was performed in the presence of glutathione peroxidase, which can reduce organic hydroperoxide to the corresponding hydroxide, the amount of 1-octen-3-ol formed from linoleic acid was decreased. At the same time, an accumulation of linoleic acid 10-hydroxide could be detected. The 10-hydroperoxide therefore seems to be an intermediate on the biosynthetic pathway.
Matsui
Kenji
K
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan. matsui@yamaguchi-u.ac.jp
Sasahara
Satomi
S
Akakabe
Yoshihiko
Y
Kajiwara
Tadahiko
T
D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D020074:Lentinula / Q000378:metabolism*; D019787:Linoleic Acid / Q000378:metabolism*; D000442:Octanols / Q000378:metabolism*
D000442:Octanols; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; C038844:1-octen-3-ol