[Dynamic changes of ultrastructure of erythrocytes in prolonged preservation at 4 degrees C].
To study the dynamic changes of ultrastructure of erythrocytes in prolonged preservation of blood with preservative fluid containing superoxide dismutase (SOD), the whole blood samples were preserved at 4 degrees C in SOD-containing solution, the morphologic changes of erythrocyte were dynamically ob served by transmission microscopy after preservation for 42, 75 and 85 days, an d the blood samples preserved in GMA solution served as control. Three variance was applied to analyze the data with SAS software. The results showed that the metamorphotic rates of erythrocyte preserved in SOD-containing solution for 42, 75 and 85 days were lower than those of erythrocytes preserved in GMA solution. Most of metamorphotic rates of erythrocyte preserved in SOD-containing solution for 42, 75 and 85 days were correspond to those of erythrocytes preserved in GMA solution for 42 days, or even lower. It is concluded that SOD-containing preservative fluid might help to maintain the normal morphology of erythrocytes in prolonged preservation at 4 degrees C.
Beijing Institute of Blood Transfusion, Beijing 100850, China. rensuping@hotmail.com
Su-Ping
SP
Ren
En-Pu
EP
Ma
Xiu-Zhen
XZ
Liu
Ying
Y
Han
An
A
Liu
Peng
P
Jin
D001793:Blood Preservation; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
D013482:Superoxide Dismutase