Significance of affinity and cooperativity in oxygen binding to hemoglobin of horse fetal and maternal blood.
The physiological significance of the position and shape of the oxygen equilibrium curve (OEC) of horse hemoglobin (Hb) is considered from the viewpoint of oxygen (O2) transport efficiency and the effectiveness of the Bohr effect. In horse fetal and maternal bloods, their physiological O2 affinities are nearly optimized with respect to the effectiveness of the Bohr shift occurring at the O2 release site, when it is measured by the change in O2 saturation per unit change in P50. With relatively low cooperativity (n=2.69) of horse Hb under physiological conditions, the effectiveness of the Bohr shift for fetal blood at O2 uptake site and maternal blood at O2 release site is high. These facts imply that the position and the cooperativity of horse Hb OEC are optimized to receive maximal benefit from the double Bohr shift. Before exercise, the position of the OEC for adult mares is nearly optimized for the effectiveness of the Bohr shift occurring at the O2 release site, whereas, at maximal exercise, the position of the OEC tends to become advantageous for O2 transport efficiency.
Graduate School of Science and Technology, Niigata University, Japan.
Yan
Y
Zhang
Keiko
K
Kobayashi
Keisuke
K
Sasagawa
Kiyohiro
K
Imai
Michiyori
M
Kobayashi
D019794:2,3-Diphosphoglycerate / Q000737:chemistry; D000818:Animals; D001692:Biological Transport; D005260:Female; D005312:Fetal Blood / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006736:Horses / Q000097:blood* / Q000378:metabolism*; D008431:Maternal-Fetal Exchange; D010100:Oxygen / Q000737:chemistry* / Q000378:metabolism; D011247:Pregnancy
D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D010100:Oxygen