Antiproliferative effects of several compounds isolated from Amburana cearensis A. C. Smith.
Amburana cearensis a common tree found in Northeastern Brazil is widely used in folk medicine. The present work evaluated the cytotoxicity of kaempferol, isokaempferide, amburoside A and protocatechuic acid isolated from the ethanol extract of the trunk bark of A. cearensis. The compounds were tested for their cytotoxicity on the sea urchin egg development, hemolysis assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay using tumor cell lines. Isokaempferide and kaempferol, but not amburoside A and protocatechuic acid, inhibited the sea urchin egg development as well as tumor cell lines, but in this assay isokaempferide was more potent than kaempferol. Protocatechuic acid was the only compound able to induce hemolysis of mouse erythrocytes, suggesting that the cytotoxicity of kaempferol and isokaempeferide was not related to membrane damage.
Costa-Lotufo
Letícia V
LV
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, P.O. Box-3157, 60430-270 Fortaleza, Ceará, Brazil. lvcosta@secrel.com.br
Jimenez
Paula C
PC
Wilke
Diego V
DV
Leal
Luzia Kalyne A M
LK
Cunha
Geanne M A
GM
Silveira
Edilberto R
ER
Canuto
Kirley M
KM
Viana
Glauce S B
GS
Moraes
Maria Elisabete A
ME
de Moraes
Manoel Odorico
MO
Pessoa
Cláudia
C
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044949:Kaempferols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010058:Oviposition / Q000187:drug effects; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012617:Sea Urchins; D012639:Seeds / Q000737:chemistry; D014197:Trees / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D062385:Hydroxybenzoates; D044949:Kaempferols; C009091:protocatechuic acid