A reagent less fluorescent sol-gel biosensor for uric acid detection in biological fluids.
The simultaneous encapsulation of a coupled uricase-peroxidase system and amplex red in a sol-gel matrix allows one to obtain a reagent-less and ready-to-use fluorescent biosensor for the accurate detection of uric acid in highly diluted biological fluids. The detection limit of the prepared biosensor was found to be 20 nM and was linear up to 1 microM. The high sensitivity found for the biosensor permitted a reliable determination of uric acid concentrations in the presence of interfering species (e.g., ascorbic acid) just by sample dilution (up to 50000 for urine and 10000 for serum and blood). The sol-gel encapsulation preserved the hierarchy of the enzyme activity as demonstrated by the performance of the fluorescent biosensor.
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, 03202 Alicante, Spain.
David
D
Martinez-Pérez
Maria L
ML
Ferrer
C Reyes
CR
Mateo
D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004800:Enzymes, Immobilized / Q000737:chemistry; D005453:Fluorescence; D005782:Gels; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D010078:Oxazines / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000097:blood* / Q000652:urine*
D004800:Enzymes, Immobilized; D005782:Gels; D007202:Indicators and Reagents; D010078:Oxazines; C470430:Amplex Red; D014527:Uric Acid; D012822:Silicon Dioxide; D009195:Peroxidase; D014503:Urate Oxidase; D001205:Ascorbic Acid