Development of a quantification system of ionic dissociative metabolites using an FT-IR/ATR method.
A simultaneous quantification system of ionic dissociative metabolites was developed using a Fourier transform mid-infrared spectroscopic method by focusing our attention on the enzyme reaction from glucose 6-phosphate to fructose 6-phosphate with phosphoglucose isomerase (PGI). We studied the pH dependency of the infrared spectra of the mixture solution for which the PGI reaction was assumed. The infrared spectra of ionic dissociative components in the mixture solution were extracted by multiple linear regression analysis under the assumption of ionic dissociation equilibrium. Additionally, we constructed a simultaneous quantification system using the extracted spectra of the ionic dissociative components on the basis of the ionic dissociation equilibrium. We could accurately estimate the pH value and the concentrations of the ionic dissociative materials in their mixture solution by using this quantification system. In addition, the stability of quantification results for a pK shift was also verified.
Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, 1515 Kamihama-cho, Tsu, Mie 514-8507, Japan.
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Pan
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Hashimoto
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Kanou
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Nakanishi
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Kameoka
D018929:Cell Culture Techniques / Q000379:methods; D002468:Cell Physiological Phenomena; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry*; D020536:Enzyme Activators; D005636:Fructosephosphates / Q000032:analysis / Q000138:chemical synthesis*; D019298:Glucose-6-Phosphate / Q000032:analysis / Q000737:chemistry*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008956:Models, Chemical; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D045424:Complex Mixtures; D020536:Enzyme Activators; D005636:Fructosephosphates; D007477:Ions; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D005956:Glucose-6-Phosphate Isomerase