Protective effects of green tea polyphenols on human HepG2 cells against oxidative damage of fenofibrate.
The aim of this work was to investigate the protective effects of green tea polyphenols on the cytotoxic effects of hypolipidemic agent fenofibrate (FF), a peroxisome proliferator (PP), in human HepG2 cells. The results showed that high concentrations of FF induced human HepG2 cell death through a mechanism involving an increase of reactive oxygen species (ROS) and intracellular reduced glutathione (GSH) depletion. These effects were partially prevented by antioxidant green tea polyphenols. The elevated expression of PP-activated receptors alpha (PPARalpha) in HepG2 cells induced by FF was also decreased by treatment with green tea polyphenols. In conclusion, this result demonstrates that oxidative stress and PPARalpha are involved in FF cytotoxicity and green tea polyphenols have a protective effect against FF-induced cellular injury. It may be beneficial for the hyperlipidemic patients who were administered the hypolipidemic drug fenofibrate to drink tea or use green tea polyphenols synchronously during their treatment.
Laboratory of Visual Information Processing, Research Centers of Brain and Cognitive Science, Institute of Biophysics, The Chinese Academy of Sciences, Beijing, China.
Hong-Li
HL
Jiao
Ping
P
Ye
Bao-Lu
BL
Zhao
D045744:Cell Line, Tumor; D011345:Fenofibrate / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D008517:Phytotherapy; D059808:Polyphenols; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D013662:Tea / Q000737:chemistry*; D014157:Transcription Factors / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D020011:Protective Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D018160:Receptors, Cytoplasmic and Nuclear; D013662:Tea; D014157:Transcription Factors; D014280:Triglycerides; D005978:Glutathione; D011345:Fenofibrate