Axonal transport of eukaryotic translation elongation factor 1alpha mRNA couples transcription in the nucleus to long-term facilitation at the synapse.
Long-term synaptic plasticity requires both gene expression in the nucleus and local protein synthesis at synapses. The effector proteins that link molecular events in the cell body with local maintenance of synaptic strength are not known. We now show that treatment with serotonin (5-HT) that produces long-term facilitation induces the Aplysia eukaryotic translation elongation factor 1alpha (Ap-eEF1A) as a late gene that might serve this coupling function in sensory neurons. Although the translation factor is induced, it is not transported into axon processes when the stimulation with 5-HT was restricted to the cell body. In contrast, its mRNA is transported when 5-HT was applied to both cell body and synapses. Intracellular injection of antisense oligonucleotides or antibodies that block the induction and expression of Ap-eEF1A do not affect the initial expression of long-term facilitation but do block its maintenance beyond 24 h. The transport of eEF1A protein and its mRNA to nerve terminals suggests that the translation factor plays a role in the local protein synthesis that is essential for maintaining newly formed synapses.
Giustetto
Maurizio
M
Center for Neurobiology and Behavior, College of Physicians and Surgeons, New York State Psychiatric Institute, and Howard Hughes Medical Institute, Columbia University, 1051 Riverside Drive, New York, NY 10032, USA.
Hegde
Ashok N
AN
Si
Kausik
K
Casadio
Andrea
A
Inokuchi
Kaoru
K
Pei
Wanzheng
W
Kandel
Eric R
ER
Schwartz
James H
JH
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001048:Aplysia; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004594:Electrophysiology; D015977:Eukaryotic Initiation Factor-1; D005091:Exons; D009474:Neurons / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000378:metabolism / Q000502:physiology*; D010448:Peptide Initiation Factors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013569:Synapses / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
D000906:Antibodies; D015977:Eukaryotic Initiation Factor-1; D016376:Oligonucleotides, Antisense; D020648:Peptide Elongation Factor 1; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; C026022:eukaryotic peptide initiation factor-1A; D012701:Serotonin; D000242:Cyclic AMP