Influence of metal ions on gene expression of BALB 3T3 fibroblasts.
It is widely recognized that metal compounds may modify gene expression. In this context, we have searched for genes whose expression may be affected by cadmium and platinum ions within the context of a cell culture system. Cadmium is well known for its carcinogenic potential while platinum is destined to become more and more interesting because of its increasing use in the automotive industries. By applying differential display to cultures of mouse fibroblast, we have identified two transcripts (acute lymphoblastic leukemia-1, All-1, and a novel gene named metal-responsive gene, MERE-1) that were responsive to platinum and cadmium ions. Moreover, further experiments with a panel of metal compounds have shown that MERE-1 was strongly induced also by La(NO3)2 and Cr(NO3)3, and to a lesser extent, by Na2CrO4 and (NH4)2TeCl6.
Department of Structural and Functional Biology, Università dell'Insubria, 3 Via Dunant, I-21100 Varese, Italy.
Raffaella
R
Cinquetti
Francesca
F
Mazzotti
Francesco
F
Acquati
Rosalba
R
Gornati
Enrico
E
Sabbioni
Roberto
R
Taramelli
Giovanni
G
Bernardini
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008670:Metals / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010984:Platinum / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D008670:Metals; D012333:RNA, Messenger; D002104:Cadmium; D010984:Platinum