Visualizing the distribution and transport of mRNAs in living cells.
We have visualized the movements of native mRNAs in living cells. Using nuclease-resistant molecular beacons, we imaged the transport and localization of oskar mRNA in Drosophila melanogaster oocytes. When the localization pattern was altered by genetic manipulation of the mRNA's 3' untranslated region, or by chemical perturbation of the intracellular tubulin network, the distribution of the fluorescence signals changed accordingly. We tracked the migration of oskar mRNA in real time, from the nurse cells where it is produced to the posterior cortex of the oocyte where it is localized. Our observations reveal the presence of a transient, and heretofore elusive, stage in the transport of oskar mRNA. Direct visualization of specific mRNAs in living cells with molecular beacons will accelerate studies of intracellular RNA trafficking and localization, just as the use of green fluorescent protein has stimulated the study of specific proteins in vivo.
Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Diana P
DP
Bratu
Byeong-Jik
BJ
Cha
Musa M
MM
Mhlanga
Fred Russell
FR
Kramer
Sanjay
S
Tyagi
D020413:3' Untranslated Regions; D000818:Animals; D001692:Biological Transport; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
D020413:3' Untranslated Regions; D029721:Drosophila Proteins; D008164:Luminescent Proteins; D012333:RNA, Messenger; D014404:Tubulin; C091675:osk protein, Drosophila; D049452:Green Fluorescent Proteins