The Neurospora crassa mus-19 gene is identical to the qde-3 gene, which encodes a RecQ homologue and is involved in recombination repair and postreplication repair.
An allele called mus-19 was identified by screening temperature-sensitive and mutagen-sensitive mutants of Neurospora crassa. The mus-19 gene was genetically mapped to a region near the end of the right arm of linkage group I, where a RecQ homologue called qde-3 had been physically mapped in the Neurospora database. Complementation tests between the mus-19 mutant and the qde-3(RIP) mutant showed that mus-19 and qde-3 were the same gene. The qde-3 genes of both mutants were cloned and sequenced; and the results showed that they have mutation(s) in their qde-3 genes. The original mus-19 and qde-3(RIP) mutants are defective in quelling, as reported for other qde-3 mutants. The mutants show high sensitivity to methyl methanesulfonate, ethyl methanesulfonate, N-methyl- N'-nitro- N-nitrosoguanidine, tert-butyl hydroperoxide, 4-nitroquinoline-1-oxide, hydroxyurea and histidine. Epistasis analysis indicated that the qde-3 gene belongs both to the uvs-6 recombination repair pathway and the uvs-2 postreplication repair pathway. The qde-3 mutation has no effect on the integration of a plasmid carrying the mtr gene by homologous recombination. In homozygous crosses, the qde-3 mutant is defective in ascospore production.
Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University, 338-8570, Saitama, Japan.
Akihiro
A
Kato
Yufuko
Y
Akamatsu
Yoshiyuki
Y
Sakuraba
Hirokazu
H
Inoue
D000251:Adenosine Triphosphatases / Q000235:genetics*; D002874:Chromosome Mapping; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics*; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D009153:Mutagens / Q000494:pharmacology; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D053484:RecQ Helicases; D011995:Recombination, Genetic; D013696:Temperature; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D005656:Fungal Proteins; D009153:Mutagens; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; D053484:RecQ Helicases; C402647:QDE-3 protein, Neurospora crassa