Comparative pathogenicity of Escherichia coli O157 and intimin-negative non-O157 Shiga toxin-producing E coli strains in neonatal pigs.
We compared the pathogenicity of intimin-negative non-O157:H7 Shiga toxin (Stx)-producing Escherichia coli (STEC) O91:H21 and O104:H21 strains with the pathogenicity of intimin-positive O157:H7 and O157:H(-) strains in neonatal pigs. We also examined the role of Stx2d-activatable genes and the large hemolysin-encoding plasmid of O91:H21 strain B2F1 in the pathogenesis of STEC disease in pigs. We found that all E. coli strains that made wild-type levels of Stx caused systemic illness and histological lesions in the brain and intestinal crypts, whereas none of the control Stx-negative E. coli strains evoked comparable central nervous system signs or intestinal lesions. By contrast, the absence of intimin, hemolysin, or motility had little impact on the overall pathogenesis of systemic disease during STEC infection. The most striking differences between pigs inoculated with non-O157 STEC strains and pigs inoculated with O157 STEC strains were the absence of attaching and effacing intestinal lesions in pigs inoculated with non-O157:H7 strains and the apparent association between the level of Stx2d-activatable toxin produced by an STEC strain and the severity of lesions.
Pre-Harvest Food Safety and Enteric Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010-0070, USA. enystrom@nadc.ars.usda.gov
Evelyn A
EA
Dean-Nystrom
Angela R
AR
Melton-Celsa
Joachim F L
JF
Pohlenz
Harley W
HW
Moon
Alison D
AD
O'Brien
D018829:Adhesins, Bacterial / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology; D002352:Carrier Proteins / Q000502:physiology*; D015169:Colony Count, Microbial; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology*; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D007422:Intestines / Q000473:pathology; D007668:Kidney / Q000473:pathology; D022603:Shiga Toxins / Q000096:biosynthesis*; D013552:Swine
D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D022603:Shiga Toxins; C073819:eaeA protein, E coli