Molecular basis of passive stress relaxation in human soleus fibers: assessment of the role of immunoglobulin-like domain unfolding.
Titin (also known as connectin) is the main determinant of physiological levels of passive muscle force. This force is generated by the extensible I-band region of the molecule, which is constructed of the PEVK domain and tandem-immunoglobulin segments comprising serially linked immunoglobulin (Ig)-like domains. It is unresolved whether under physiological conditions Ig domains remain folded and act as "spacers" that set the sarcomere length at which the PEVK extends or whether they contribute to titin's extensibility by unfolding. Here we focused on whether Ig unfolding plays a prominent role in stress relaxation (decay of force at constant length after stretch) using mechanical and immunolabeling studies on relaxed human soleus muscle fibers and Monte Carlo simulations. Simulation experiments using Ig-domain unfolding parameters obtained in earlier single-molecule atomic force microscopy experiments recover the phenomenology of stress relaxation and predict large-scale unfolding in titin during an extended period (> approximately 20 min) of relaxation. By contrast, immunolabeling experiments failed to demonstrate large-scale unfolding. Thus, under physiological conditions in relaxed human soleus fibers, Ig domains are more stable than predicted by atomic force microscopy experiments. Ig-domain unfolding did not become more pronounced after gelsolin treatment, suggesting that the thin filament is unlikely to significantly contribute to the mechanical stability of the domains. We conclude that in human soleus fibers, Ig unfolding cannot solely explain stress relaxation.
Veterinary and Comparative Anatomy, Pharmacology and Phisiology,[correction Physiology] Washington State University, Pullman, Washington 99164-6520, USA.
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Trombitás
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Wu
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McNabb
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Greaser
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Kellermayer
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Labeit
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Granzier
D000222:Adaptation, Physiological / Q000502:physiology; D001665:Binding Sites; D002478:Cells, Cultured; D003198:Computer Simulation; D064211:Connectin; D004548:Elasticity; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D009124:Muscle Proteins / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure*; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D011494:Protein Kinases / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D012518:Sarcomeres / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure*; D013314:Stress, Mechanical; D013329:Structure-Activity Relationship; D014783:Viscosity
D064211:Connectin; D007136:Immunoglobulins; D009124:Muscle Proteins; C577285:TTN protein, human; D011494:Protein Kinases