In vitro inhibitory effects of tea polyphenols on the proliferation of Chlamydia trachomatis and Chlamydia pneumoniae.
In vitro inhibitory effects of tea polyphenols on Chlamydia trachomatis and C. pneumoniae were investigated. A product of tea polyphenols, Polyphenon 70S was used. Chlamydial strains used were C. trachomatis D/UW-3/Cx and L(2)/434/Bu, and C. pneumoniae AR-39 and AC-43 strains. HeLa229 cells and HL cells were used for cultivation of C. trachomatis and C. pneumoniae, respectively. In the post-inoculation method, no inclusions of C. trachomatis were observed at 0.5 mg/ml of Polyphenon 70S. However, the toxicity of Polyphenon 70S was noted in HeLa229 cells and HL cells at a concentration of 0.25 mg/ml. In the pre-inoculation method, no toxic effects of Polyphenon 70S on the cells were noted. Complete inhibition of C. trachomatis D and L(2) was noted at concentrations of 1.6 and 0.4 mg/ml, respectively. With C. pneumoniae strains, the end points were 0.8 and 1.6 mg/ml for AR-39 and AC-43, respectively. Our findings encouraged the application of tea polyphenols for topical usage.
Department of Pediatrics, Saitama Medical School, Iruma, Saitama 350-0495, Japan. benyama@saitama-med.ac.jp
Tsutomu
T
Yamazaki
Miyuki
M
Inoue
Nozomu
N
Sasaki
Toshikatsu
T
Hagiwara
Toshio
T
Kishimoto
Sadashi
S
Shiga
Motohiko
M
Ogawa
Yukihiko
Y
Hara
Takaaki
T
Matsumoto
D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000254:growth & development; D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D013662:Tea
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea