Tumor necrosis factors in a murine model of allergic peritonitis: effects on eosinophil accumulation and inflammatory mediators' release.
In allergic disorders, the role of tumor necrosis factors (TNF) is not well established. We investigated the role of TNF in allergic peritonitis induced by ovalbumin (OVA) challenge in double TNF (TNF-alpha(-/-)/lymphotoxin-alpha(-/-)) knock out (TNF-KO) mice. In the peritoneal lavage of TNF-KO mice, mast cell number and histamine level (radioenzymatic assay) were similar to that in wild type (WT) mice. TNF-alpha (ELISA) and histamine were increased 1 h after challenge in WT mice. However, three days later eosinophil number and eosinophil peroxidase (EPO) levels (colorimetric-enzymatic assay) were found to be lower in TNF-KO mice. A second challenge three days after the first, increased EPO, histamine and IL-6 (ELISA) but did not alter eosinophil and mast cell numbers in both types of mice. On the other hand histamine and IL-6 were higher, while EPO was lower in TNF-KO mice. In conclusion, our findings show that TNF is involved in eosinophil accumulation and inflammatory mediators' release in a murine model of allergy.
Department of Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, P.O. Box 12065, Jerusalem 91120, Israel.
Vladislav
V
Temkin
Dalia
D
Pickholtz
Francesca
F
Levi-Schaffer
D000818:Animals; D004195:Disease Models, Animal; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000473:pathology; D006632:Histamine / Q000378:metabolism; D006967:Hypersensitivity / Q000378:metabolism* / Q000473:pathology*; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000009:adverse effects; D010538:Peritonitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D010544:Peroxidases / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D006632:Histamine; D010047:Ovalbumin; D047209:Eosinophil Peroxidase; D010544:Peroxidases