Expression and production of bioactive human interleukin-18 in transgenic tobacco plants.
The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-gamma on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g(-1) tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.
National Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, PR China.
Bin
B
Zhang
Ying-Hua
YH
Yang
Yong-Ming
YM
Lin
Qing
Q
Rao
Guo-Guang
GG
Zheng
Ke-Fu
KF
Wu
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D018506:Gene Expression Regulation, Plant; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D007938:Leukemia / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic
D020382:Interleukin-18; D011994:Recombinant Proteins