Lidocaine attenuates monocyte chemoattractant protein-1 production and chemotaxis in human monocytes: possible mechanisms for its effect on inflammation.
The recruitment and activation of peripheral blood monocytes are potentially critical regulatory events for the control of inflammation. Increased levels of monocyte chemoattractant protein (MCP)-1 have been reported in several inflammatory disorders. In this study, we examined the effect of lidocaine on lipopolysaccharide-stimulated MCP-1 secretion and MCP-1 induced chemotaxis in a human monocytic cell line, THP-1. Lidocaine inhibited lipopolysaccharide-induced MCP-1 production as well as messenger RNA expression in a dose-dependent manner. Furthermore, we demonstrated that lidocaine suppressed MCP-1-induced chemotaxis and peak cytosolic-free calcium in THP-1 cells. These results suggest that lidocaine may modulate MCP-1 production and MCP-1-induced activation in inflammatory cells.


CONCLUSIONS
Monocyte chemoattractant protein-1 (MCP-1) plays important roles in the inflammatory processes. Lidocaine may modulate MCP-1-induced monocyte response, as reflected by chemotaxis, cytosolic-free calcium, and lipopolysaccharide-induced MCP-1 production by human monocytic THP-1 cells.
*Departments of Anesthesiology and †Division of Cardiovascular Surgery, Tri-Service General Hospital; and ‡Departments of Biochemistry, National Defense Medical Center, National Defense University, Taipei, Taiwan, Republic of China.
Chi-Yuan
CY
Li
Chien-Sung
CS
Tsai
Ping-Ching
PC
Hsu
Sheau-Huei
SH
Chueh
Chih-Shung
CS
Wong
Shung-Tai
ST
Ho
D000779:Anesthetics, Local / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D017931:DNA Primers; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008012:Lidocaine / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
D000779:Anesthetics, Local; D018932:Chemokine CCL2; D017931:DNA Primers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D008012:Lidocaine; D002118:Calcium