DNA vaccine encoding pertussis toxin S1 subunit induces protection against Bordetella pertussis in mice.
Pertussis toxin (PT) is the major virulence factor of Bordetella pertussis, and detoxified PT is a crucial antigen of acellular pertussis vaccine. Here, plasmid DNA expressing the pertussis toxin S1 subunit (pcDNA/S1) of B. pertussis was evaluated for immunogenicity and for the ability to induce protection against PT challenge or B. pertussis infection in mice. The gene gun delivery of pcDNA/S1, performed by inserting the S1 gene into a mammalian expression vector, successfully induced anti-PT IgG antibody production. Immunization of mice with pcDNA/S1 significantly inhibited leukocytosis-promoting activity caused by PT or B. pertussis. In addition, pcDNA/S1 induced significant protection against intracerebral challenge with a lethal dose of B. pertussis. The results of the present study demonstrated that a DNA vaccine encoding the PT-S1 subunit induced protection against B. pertussis infection in mice. Thus, this vaccine preparation is potentially applicable for the production of novel vaccines against B. pertussis infection.
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan. kamachi@nih.go.jp
Kazunari
K
Kamachi
Toshifumi
T
Konda
Yoshichika
Y
Arakawa
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000276:immunology; D005260:Female; D015151:Immunoblotting; D007964:Leukocytosis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D037342:Pertussis Toxin / Q000235:genetics* / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology*; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D018076:DNA, Complementary; D010567:Pertussis Vaccine; D019444:Vaccines, DNA; C083388:pertussis toxin, S1 subunit; D037342:Pertussis Toxin