[Cytotoxicity of DMSO for MRC5, Chang liver and CV1 cells evaluated in vitro by LK, MTT and NR assays].
Evaluation of chemicals cytotoxicity plays fundamental role in many in vitro investigations. The way of assessment of cytotoxicity depend on aim of study, characteristic of used cells and mode of action of investigated chemicals. The principal aspect of these investigations is validation of used method. In this paper validation of three different cytotoxicity assays is presented: total cell number measurement (LK), microplate assay measured mitochondrial dehydrogenase activity (MTT) and colorimetric assay measured ability of live cell to uptake neutral red (NR). The investigation was performed on different cells (MRC5, CV1 i Chang Liver) with DMSO as reference agent.
Zakład Wirusologii PZH w Warszawie. jsiennicka@pzh.gov.pl
Joanna
J
Siennicka
Włodzimierz
W
Gut
Anna
A
Zuk
Bogumiła
B
Litwińska
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D003124:Colorimetry; D004396:Coloring Agents / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008928:Mitochondria / Q000201:enzymology*; D009499:Neutral Red / Q000378:metabolism*; D010088:Oxidoreductases / Q000032:analysis*
D004396:Coloring Agents; D009499:Neutral Red; D010088:Oxidoreductases; D004121:Dimethyl Sulfoxide