Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10.
Cytokine production by phagocytic cells is an important component of the immune response to infection with a variety of pathogens. Neutrophils are phagocytic cells capable of expressing interleukin (IL)-12 and IL-10 in response to infection with parasites, fungi, and bacteria. These cytokines are postulated to play important roles in the immune response during measles. Neutrophils isolated from naive or measles virus (MV)-infected rhesus macaques expressed IL-12 and IL-10 following in vitro stimulation with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). Stimulated neutrophils secreted proportionally more of the biologically active IL-12 heterodimer p70 and more IL-10 than similarly stimulated peripheral blood mononuclear cells (PBMCs). During MV infection, the number of IL-12 and IL-10-producing neutrophils and monocytes initially decreased. Subsequently, IL-12 levels were restored, and IL-10 expression rose above baseline in both cell types. Increased IL-10 production by neutrophils and monocytes during MV infection may play a role in measles-induced immunosuppression.
Hoffman
Scott J
SJ
W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Polack
Fernando P
FP
Hauer
Debra A
DA
Griffin
Diane E
DE
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008253:Macaca mulatta; D008457:Measles / Q000235:genetics / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12