The mechanism of 7,8-diaminopelargonate synthase; the role of S-adenosylmethionine as the amino donor.
The product of the first transamination step in the reaction catalysed by diaminopelargonate (DAPA) synthase has been shown to be 4-(S-adenosyl)-2-oxobutanoate, which has been trapped as the corresponding alcohol.
Department of Chemistry, Joseph Black Building, The University of Edinburgh, West Mains Road, Edinburgh, UK EH9 3JJ.
Rachel S
RS
Breen
Dominic J
DJ
Campopiano
Scott
S
Webster
Mhairi
M
Brunton
Rory
R
Watt
Robert L
RL
Baxter
D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology; D008956:Models, Chemical; D010084:Oxidation-Reduction; D012436:S-Adenosylmethionine / Q000737:chemistry*; D000637:Transaminases / Q000737:chemistry*
D012436:S-Adenosylmethionine; C020029:7,8-diaminopelargonic acid aminotransferase; D000637:Transaminases