Antifungal combinations against Candida albicans biofilms in vitro.
Candida biofilms display increased resistance to most antifungal agents. We have evaluated the efficacy of combinations of fluconazole (FLC), amphotericin B, and caspofungin (CSP) against Candida albicans biofilms in vitro. Indifference was observed for all the combinations of paired antifungal agents when a checkerboard titration method was used. Time-kill experiments revealed an antagonistic effect of high FLC doses with CSP.
Division of Infectious Diseases, Department of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA.
Stefano P
SP
Bachmann
Gordon
G
Ramage
Kacy
K
VandeWalle
Thomas F
TF
Patterson
Brian L
BL
Wickes
José L
JL
López-Ribot
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D000077336:Caspofungin; D004338:Drug Combinations; D054714:Echinocandins; D015725:Fluconazole / Q000494:pharmacology; D055666:Lipopeptides; D010455:Peptides / Q000494:pharmacology; D010456:Peptides, Cyclic; D013778:Tetrazolium Salts
D000935:Antifungal Agents; D004338:Drug Combinations; D054714:Echinocandins; D055666:Lipopeptides; D010455:Peptides; D010456:Peptides, Cyclic; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D000666:Amphotericin B; D015725:Fluconazole; D000077336:Caspofungin