Functional characterization of a peroxisome proliferator response-element located in the intron 3 of rat peroxisomal thiolase B gene.
Expression of the rat peroxisomal 3-ketoacyl-CoA thiolase gene B is induced by peroxisome proliferators. Although a sequence element like a peroxisome proliferator-activated receptor (PPAR)-binding site is located in the promoter region of this gene, we previously found that this element is competent for the activation by hepatocyte nuclear factor-4, but not functional with PPARalpha. We describe here a new peroxisome proliferator-response element located in the intron 3 (+1422/+1434) that binds in vitro the PPARalpha/retinoid X receptor alpha heterodimer and confers the induction by PPARalpha in transfection assays. We propose a model of regulation of the rat thiolase B gene involving those elements in the promoter and intron 3.
Hansmannel
Franck
F
LBMC (GDR-CNRS No. 2583), Université de Bourgogne, Dijon, France.
Clémencet
Marie-Claude
MC
Le Jossic-Corcos
Catherine
C
Osumi
Takashi
T
Latruffe
Norbert
N
Nicolas-Francés
Valérie
V
D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D051557:Hepatocyte Nuclear Factor 4; D007438:Introns; D008099:Liver / Q000201:enzymology*; D020025:Peroxisome Proliferators / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology* / Q000235:genetics*; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D051557:Hepatocyte Nuclear Factor 4; D020025:Peroxisome Proliferators; D010750:Phosphoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D000101:Acetyl-CoA C-Acetyltransferase