Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation.
BACKGROUND
Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro.


METHODS
Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry.


RESULTS
Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100beta, and neurofilament.


CONCLUSIONS
A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.
Brunet
Jean François
JF
Department of Neurosurgery of Lausanne and Geneva University Medical School, Bugnon 46, 1011, Lausanne, Switzerland. Jean-Francois.Brunet@chuv.hospvd.ch
Pellerin
Luc
L
Magistretti
Pierre
P
Villemure
Jean Guy
JG
D000328:Adult; D001921:Brain / Q000166:cytology* / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D017690:Cell Transplantation; D015925:Cryopreservation / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D016900:Neurofilament Proteins / Q000378:metabolism; D009584:Nitrogen; D009926:Organ Preservation / Q000379:methods*; D009418:S100 Proteins / Q000096:biosynthesis; D013696:Temperature; D013997:Time Factors; D014182:Transplantation, Autologous
D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D009418:S100 Proteins; C110294:S100A1 protein; D009584:Nitrogen