Characterization of the glucose-6-phosphate isomerase gene in Phytophthora infestans reveals the presence of multiple alleles.
Glucose-6-phosphate isomerase (GPI) plays a key role in both glycolysis and gluconeogenesis. Isoforms of GPI are common, and therefore, its isozyme pattern is widely used to characterize isolates of Phytophthora infestans. Despite the importance of GPI in P. infestans studies, the gene encoding this enzyme has not yet been characterized. Furthermore, it has been suggested that P. infestans contains multiple copies of the gene but this hypothesis remains to be demonstrated. We have cloned and characterized GPI in various isolates of P. infestans as well as in several species of the genus Phytophthora. The gene contains 1671bp and encodes a protein with a predicted molecular weight of 60.8kDa. Multiple different alleles were identified and Southern analysis indicated certain P. infestans isolates carry several copies of the gene. Phylogenetic analysis revealed that P. infestans GPI is most closely related to sequences from Toxoplasma gondii, Arabidopsis thaliana, and Clarkia lewisii.
Ospina-Giraldo
M D
MD
USDA/ARS/Vegetable Laboratory, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA.
Jones
R W
RW
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005843:Geography; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D010802:Phylogeny; D010838:Phytophthora / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D017931:DNA Primers; D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase