Molecular detection of cashew husk (Anacardium occidentale) adulteration in market samples of dry tea (Camellia sinensis).
Species-specific PCR primers were developed from intergenic spacer regions of 5S ribosomal RNA genes and used successfully in the detection of adulteration of cashew husk (Anacardium occidentale L.) in tea [Camellia sinensis (L.) O. Kuntze] samples. This is the first report of detecting adulteration in tea using molecular tools. Application of this approach in detecting adulteration of other biological materials in tea, medicinal herbs and the composition of admixtures of ayurvedic herbs has been discussed.
Dhiman
Bandana
B
Division of Biotechnology, Institute of Himalayan Bioresource Technology, Palampur, India.
Singh
Mahipal
M
D031021:Anacardium / Q000235:genetics*; D028241:Camellia sinensis / Q000737:chemistry*; D017931:DNA Primers; D005503:Food Additives / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
D017931:DNA Primers; D005503:Food Additives; D012341:RNA, Ribosomal, 5S