Metabonomics: NMR spectroscopy and pattern recognition analysis of body fluids and tissues for characterisation of xenobiotic toxicity and disease diagnosis.
Global profiling tools are required to fully understand the impact of genetic modifications and toxicological interventions on the network of transcripts, proteins and metabolites found within a cell, tissue or organism. High-resolution 1H NMR spectroscopy in conjunction with statistical pattern recognition is one such technique, referred to as metabonomics or metabolomics, which is increasingly being used to globally profile metabolites. This review examines analytical advances in NMR spectroscopy that have aided this development including high-resolution magic angle spinning NMR spectroscopy, cryogenically cooled probes and high-through put systems. This has allowed the approach to identify genetically modified yeast strains and distinguish both disease presence and severity in coronary heart disease.
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK. jlg40@mole.bio.cam.ac.uk
Julian L
JL
Griffin
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D001923:Brain Chemistry; D003327:Coronary Disease / Q000175:diagnosis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D030781:Organisms, Genetically Modified / Q000235:genetics; D015262:Xenobiotics / Q000633:toxicity*
D015262:Xenobiotics