[Study on the correlation between the different papillomavirus type and telomerase in cervical cancer].
OBJECTIVE
To define a correlation between different human papillomavirus (HPV) types and telomerase activity in cervical cancer.


METHODS
Telomerase activity was detected by TRAP-PCR, and different HPV type was determined by PCR in 83 cervical cancer, 47 cervical intraepithelial neoplasia (CIN) and 10 normal cervix cases.


RESULTS
With regard to positive rates of telomerase and HPV 16/18: the results were cervical cancer > CIN > normal cervix, CIN III > CIN I, II; with regard to HPV 6/11 positive rate: the results showed CIN I, II > CIN III. Positive rates of telomerase cervical cancer and HPV were bearing on grading and staging, but they did not correlate with histologic subtypes. Positive rate of HPV 6/11 had nothing to do with grading, staging and histologic patterns. On expression strength of telomerase and HPV 16/18: the results showed cervical cancer > CIN, CIN III > CIN I, II. Regard to HPV 6/11'expression strength: the results showed CIN I, II > CIN III, CIN > cervical carcinoma.


CONCLUSIONS
HPV 16/18 infection seemed to have played an important role in carcinogenesis of cervical lesions by activation of telomerase.
Department of Gynecology, the Third Affiliated Hospital Harbin Medical University, Harbin 150040, China.
Wen
W
Lv
Guang-Mei
GM
Zhang
Li-Hua
LH
Sui
Jing
J
Wang
D005260:Female; D006801:Humans; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000302:isolation & purification*; D019098:Telomerase / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000201:enzymology / Q000473:pathology / Q000821:virology*
D019098:Telomerase