Expression of angiopoietic factors in normal and type-I diabetes human placenta: a pilot study.
OBJECTIVE
Morphological changes of blood vessel wall have been described in placenta from pregnancies complicated by diabetes mellitus type-I.


METHODS
We measured mRNA expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and 2 (Ang-1 and Ang-2), their receptors VEGFR-1, VEGFR-2, Tie-2, fibroblast growth factor 2 (FGF-2), and its receptor FGF-2R in placental tissue of diabetes type-I patients, in normal term placenta, and endometrium of non-pregnant women by real time reverse transcriptase PCR.


RESULTS
The expression of Ang-2 and VEGFR-1 mRNAs was significantly higher in placenta (P<or=0.01). The expression of VEGF and VEGFR-2 mRNAs was significantly lower in normal term or diabetes type-I placenta compared to human endometrium (P<or=0.01). We did not detect a significant difference in the expression of Ang-1, Ang-2, Tie-2, VEGF, VEGFR-1, VEGFR-2, FGF-2, and FGF-2R in normal and diabetes type-I placenta.


CONCLUSIONS
These data show differential expression of Ang-2, VEGFR-1, VEGF, and VEGFR-2 in placenta and endometrium. The expression of Ang-1, Ang-2, Tie-2, VEGF, VEGFR-1, VEGFR-2, FGF-2, and FGF-2R was not different in normal and type-I diabetes placenta.
Janota
Jan
J
Institute for the Care of Mother and Child, Podolske nabrezi 157, Praha 4, 140 00, Czech Republic. janjanota@yahoo.com
Pomyje
Jirí
J
Toth
Dusan
D
Sosna
Ondrej
O
Zivný
Jaroslav
J
Kuzel
David
D
Stranák
Zbynek
Z
Necas
Emanuel
E
Zivný
Jan H
JH
D042683:Angiopoietin-1 / Q000235:genetics; D042702:Angiopoietin-2 / Q000235:genetics; D042682:Angiopoietins / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D051497:Receptor, Fibroblast Growth Factor, Type 2; D042787:Receptor, TIE-2 / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics
C467630:ANGPT1 protein, human; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D042682:Angiopoietins; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; C496353:FGFR2 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2; D042787:Receptor, TIE-2; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2