Synthesis of 15N-enriched pseudouridine derivatives.
[reaction: see text]. A procedure for the chemical synthesis of [3-15N]-labeled pseudouridine and a methylated derivative was developed. A suitably protected pseudouridine precursor was nitrated at N3 followed by treatment with 15NH4Cl to afford the 15N-labeled product in six steps with a 20% yield. This methodology will allow for the production of RNAs with [3-15N]pseudouridine and [3-15N-methyl]pseudouridine at specific locations.
Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
Jean-Paul
JP
Desaulniers
Bashar
B
Ksebati
Christine S
CS
Chow
D000643:Ammonium Chloride / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009587:Nitrogen Isotopes / Q000737:chemistry*; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000737:chemistry; D012313:RNA / Q000737:chemistry
D009587:Nitrogen Isotopes; D000643:Ammonium Chloride; D011560:Pseudouridine; D012313:RNA