Effect of methyl alcohol on the morphology and conformational characteristics of silk sericin.
Effects of methyl alcohol on the morphology and conformational characteristics of silk sericin (SS) were studied. Scanning electron microscope showed that morphology of SS lyophilized was dramatically changed from sponge-like structure to spherical fine particle type. X-ray diffraction method, infrared spectroscopy, and differential scanning calorimetry showed that the conformation of SS was random coil structure regardless of the addition of methyl alcohol. On the other hand, circular dichroism showed that the molecular states of SS were more densely packed.
Lee
KwangGill
K
Department of Sericulture and Entomology, National Institute of Agriculture and Technology, 441-100, Suwon, South Korea
Kweon
HaeYong
H
Yeo
Joo Hong
JH
Woo
Soon Ok
SO
Lee
Yong Woo
YW
Cho
Chong-Su
CS
Kim
Ki Ho
KH
Park
Young Hwan
YH
D000818:Animals; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002942:Circular Dichroism; D019476:Insect Proteins / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D047030:Sericins; D047011:Silk; D013055:Spectrophotometry, Infrared / Q000379:methods; D014961:X-Ray Diffraction
D019476:Insect Proteins; D010456:Peptides, Cyclic; D047030:Sericins; D047011:Silk; D000432:Methanol