Expression of cell adhesion molecules in the adriamycin-induced esophageal atresia rat model.
Cell adhesion molecules are well-known membrane glycoproteins widely expressed during embryonic development that play a crucial role in cell division, migration and differentiation. We investigated the cell-matrix relationship using N-CAM and pan-cadherin adhesion molecules in the adriamycin-induced esophageal atresia (EA) rat model in the hope of finding a clue to the mechanisms of this unique anomaly.Time-mated pregnant Sprague-Dawley rats were given either saline or adriamycin on days 8 and 9 of gestation. Embryos were harvested on the 18th day of gestation. Esophageal specimens obtained from adriamycin-exposed embryos with (EA+) or without esophageal atresia (EA-) and from saline-exposed embryos were immunostained with N-CAM and pan-cadherin primary antisera. The esophageal specimens from control and EA- groups revealed similar immunostaining properties: weak N-CAM and pan-cadherin immunoreactivity. In contrast, the EA+ group showed intense immunoreactivity. Our study demonstrated an increased synthesis of N-CAM and pan-cadherin in the epithelial cells of the atretic esophagus and trachea. These results suggest that embryonic cell-cell and cell-matrix interactions may play a crucial role in the development of adriamycin-induced EA.
Faculty of Medicine, Department of Pediatric Surgery, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey. tugaym@hotmail.com
Melih
M
Tugay
Serdar
S
Filiz
Hakki
H
Dalçik
B Haluk
BH
Güvenç
Cannur
C
Dalçik
Mevlit
M
Korkmaz
Selami
S
Sozubir
D000818:Animals; D015820:Cadherins / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism*; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004317:Doxorubicin; D004933:Esophageal Atresia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004947:Esophagus / Q000002:abnormalities* / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D019006:Neural Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens
D015820:Cadherins; D015815:Cell Adhesion Molecules; D019006:Neural Cell Adhesion Molecules; D013723:Teratogens; D004317:Doxorubicin