Time series analysis demonstrates the absence of pulsatile hyphal growth.
Hyphal tip growth has been previously reported as pulsatile, defined as regularly alternating fast and slow rates of extension. The growth of pollen tubes, and hyphae of Neurospora crassa and Saprolegnia ferax were analysed using high spatial and temporal resolution. By using long (100-500 s) records of growth rate, sampled every second, it was possible to apply rigorous statistical analysis of the time series. As previously demonstrated, pollen tubes can show pulsatile growth, detectable with this system. In contrast, hyphal growth rates do not show any evidence of pulsatile growth; instead, growth rates appear to fluctuate randomly. It is concluded that pulsatile growth is not a common feature of hyphal tip growth.
Sampson
Karina
K
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
Lew
Roger R
RR
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
Heath
I Brent
IB
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
D005583:Fourier Analysis; D025301:Hyphae / Q000254:growth & development* / Q000648:ultrastructure; D007700:Kinetics; D009492:Neurospora crassa / Q000254:growth & development*; D010507:Periodicity; D063245:Plant Development; D011058:Pollen / Q000254:growth & development / Q000648:ultrastructure; D044762:Saprolegnia / Q000254:growth & development*; D013997:Time Factors