Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis).
A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.
Mekuria
Genet
G
School of Agriculture and Wine, The University of Adelaide, Waite Campus, PMB #1, Glen Osmond, SA 5064, Australia.
Ramesh
Sunita A
SA
Alberts
Evita
E
Bertozzi
Terry
T
Wirthensohn
Michelle
M
Collins
Graham
G
Sedgley
Margaret
M
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019179:Ilarvirus / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D012367:RNA, Viral