[Effect of erythrocyte preserved for different lengths of time on anti-D antibody identification with three blood matching tests].
The specificity of the antigens and length of preservation time of erythrocytes are the interfering factors in blood group serological tests. In order to clarify the influence of preservation time of erythrocytes on the blood matching test, the titers of anti-D antibody were detected with papain method, BioVue cross matching card and DianaGel cross matching card in 7 series of panel red blood cells preserved for various length of time (0 to 9 months). The results showed that the titer of micro-column gel test (DianaGel card) was one tube higher than that of column agglutinating test (BioVue card). The titer of erythrocytes preserved for 9 months was as high as 256 tested by DianaGel card, but it was only 2 by papain method in the same anti-serum. It is suggested that there was no obvious difference between the results of micro-column gel test and column agglutinating test, and titer of papain method was the lowest.
Department of Blood Transfusion, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Rui-Qing
RQ
Xiao
Wu-Cun
WC
Lin
Dan
D
Xu
Jie
J
Zeng
Jian-Jun
JJ
Wu
Shu-Ming
SM
Zhao
D001788:Blood Grouping and Crossmatching; D001793:Blood Preservation; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D018029:Rho(D) Immune Globulin; D013997:Time Factors
D007518:Isoantibodies; C061961:RHO(D) antibody; D018029:Rho(D) Immune Globulin