[Induction of biochemical composition in Heterosigma akashiwo under Fe stress].
The iron-stress mediated effects on biochemical constituents of red tide alga H. akashiwo were examined in Fe-replete and iron deficiency, and low iron batch cultures. The content of all pigments decreased under iron limitation, and the cellular chlorophyll a concentration decreased by more than 2-fold compared to that under iron replete condition. This change in Chl a was accompanied by a corresponding decrease in Chl c per cell, resulting in no trend in the ratio of Chl c to Chl a. The cellular carotenoids content decreased by more than 1.5-fold compared to that under iron replete condition. As a consequence, carotenoids/chlorophyll a ratio increased in Fe-deficient cells. Carbohydrate content was reduced under iron stress, and total proteins decreased with the decrease of iron concentration. A crude fractionation of the soluble proteins demonstrated that 17 kD and 55 kDa proteins in soluble fraction were induced.
National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China. lidongxia1974@yahoo.com
Dongxia
D
Li
Wei
W
Cong
Zhaoling
Z
Cai
Dingji
D
Shi
Fan
F
Ouyang
D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis*; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism; D057097:Harmful Algal Bloom; D007501:Iron / Q000378:metabolism*; D010860:Pigments, Biological / Q000032:analysis*
D010860:Pigments, Biological; D002734:Chlorophyll; D002338:Carotenoids; D007501:Iron