Recombinant ganglioside GM2 synthase--expression in insect cells and enzyme assay.
Kekulé-Institute für Organische Chemie and Biochemie, Universitat Bonn, Gerhard Domagk Strasse 1, Bonn 53121, Germany.
Michaela
M
Wendeler
Helmut
H
Reilaender
Joerg
J
Hoernschemeyer
Guenter
G
Schwarzmann
Thomas
T
Kolter
Konrad
K
Sandhoff
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002240:Carbohydrate Sequence; D002460:Cell Line; D005678:G(M2) Ganglioside / Q000096:biosynthesis / Q000737:chemistry; D015870:Gene Expression; D015151:Immunoblotting; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D018411:Spodoptera; D013379:Substrate Specificity; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
D011994:Recombinant Proteins; D005678:G(M2) Ganglioside; D014536:Uridine Diphosphate N-Acetylgalactosamine; D017350:N-Acetylgalactosaminyltransferases; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase