Biosynthesis of vitamin B1 in yeast. Derivation of the pyrimidine unit from pyridoxine and histidine. Intermediacy of urocanic acid.
Incorporation studies with 13C-, 15N-, and 2H-labeled substrates, followed by NMR analysis, show that the pyrimidine unit of thiamin (Vitamin B1) originates from a C5N fragment, derived from C-2',2,N,C-6,5,5' of pyridoxol (Vitamin B6) and an N-C-N fragment derived from L-histidine. Urocanic acid serves as an intermediate on the route of the N-C-N fragment of histidine into the thiamin pyrimidine.
Zeidler
Johannes
J
Department of Chemistry, McMaster University, Hamilton, Ontario, Canada L8S 4M1.
Sayer
Brian G
BG
Spenser
Ian D
ID
D002175:Candida / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006639:Histidine / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011725:Pyridines / Q000378:metabolism*; D011736:Pyridoxine / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis* / Q000378:metabolism; D014560:Urocanic Acid / Q000378:metabolism*; D025101:Vitamin B 6 / Q000378:metabolism
D011725:Pyridines; D006639:Histidine; D025101:Vitamin B 6; D014560:Urocanic Acid; D005947:Glucose; D011736:Pyridoxine; C023666:pyridine; D005990:Glycerol; D013831:Thiamine