Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.
Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.
Sinclair
Alistair
A
Antibiotic Resistance Monitoring and Reference Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency-Colindale, London NW9 5HT, United Kingdom.
Arnold
Catherine
C
Woodford
Neil
N
D000081:Acetamides / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects*; D005798:Genes, Bacterial / Q000235:genetics; D000069349:Linezolid; D009154:Mutation / Q000235:genetics; D023303:Oxazolidinones / Q000494:pharmacology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid