Free radical scavenging efficiency of Nano-Se in vitro.
In this study, we showed that smaller size particles of Nano-Se have better scavenging effects on the following free radicals: carbon-centered free radicals (R*) generated from 2,2'-azo-bis-(2-amidinopropane) hydrochloride (AAPH), the relatively stable free radical 1,1-diphenyl-2-picryhydrazyl (DPPH), the superoxide anion (O2*-) generated from the xanthine/xanthine oxidase (X/XO) system, singlet oxygen (1O2) generated by irradiated hemoporphyrin. Furthermore, the three sizes of Nano-Se studied also show protective effects against the oxidation of DNA. The three samples all have potential size-dependent characteristics on scavenging the free radicals. Although in this study we regarded Nano-Se as a whole without considering interactions between BSA and the red selenium nano-particles, there is the possibility that the apparent free radical scavenging effects may be partially contributed by such interactions.
Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P R China.
Bo
B
Huang
Jinsong
J
Zhang
Jingwu
J
Hou
Chang
C
Chen
D000578:Amidines / Q000737:chemistry; D001713:Biphenyl Compounds; D004247:DNA / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry*; D036103:Nanotechnology; D009569:Nitric Oxide / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D012643:Selenium / Q000737:chemistry* / Q000494:pharmacology; D012710:Serum Albumin, Bovine; D026082:Singlet Oxygen / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D000578:Amidines; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D013481:Superoxides; D026082:Singlet Oxygen; D012710:Serum Albumin, Bovine; D009569:Nitric Oxide; C046728:2,2'-azobis(2-amidinopropane); D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium