A biologically active fructan from the roots of Arctium lappa L., var. Herkules.
From the roots of Arctium lappa L., var. Herkules a low-molecular-weight fructofuranan of the inulin-type has been isolated by water extraction and ethanol precipitation, followed by ion-exchange chromatography and gel filtration of the crude precipitate. The methods employed in structural determination were methylation analysis and 1H and 13C NMR spectral measurements. In tests for antitussive activity in cats the fructan was found to be equally active as some non-narcotic, synthetic preparations used in clinical practice to treat coughing, and in mitogenic and comitogenic tests its biological response was comparable to that of the commercial Zymosan immunomodulator.
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. chemkard@savba.sk
A
A
Kardosová
A
A
Ebringerová
J
J
Alföldi
G
G
Nosál'ová
S
S
Franová
V
V
Hríbalová
D000818:Animals; D000996:Antitussive Agents / Q000737:chemistry / Q000494:pharmacology*; D031170:Arctium / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002415:Cats; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003371:Cough / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D005260:Female; D005630:Fructans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008934:Mitogens / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
D000996:Antitussive Agents; D005630:Fructans; D008934:Mitogens; D015054:Zymosan