pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein.
We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G+C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were homologous to those of other bacterial plasmid Rep proteins, the degree of similarity was very low. A deletion analysis showed that the Rep protein was not required for the replication of pHP489 in its H. pylori host, but the host replication machinery was needed.
Department of Microbiology, Gyeongsang National University College of Medicine, 90 Chiram-dong, Jinju, Gyeongsangnam-do 660-751, Republic of Korea.
Jae-Young
JY
Song
Seong-Gyu
SG
Park
Hyung-Lyun
HL
Kang
Woo-Kon
WK
Lee
Myung-Je
MJ
Cho
Jeong-Uck
JU
Park
Seung-Chul
SC
Baik
Hee-Shang
HS
Youn
Gyung-Hyuck
GH
Ko
Kwang-Ho
KH
Rhee
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; C481641:RepH protein, Helicobacter pylori