[Evaluation of cellular immune response in patients with recurrent candidiasis].
Recurrent cutaneous or mucosal candidiasis is characterized by the occurrence of at least four candidiasis episodes within a one-year period. The factors involved in recurrence of infection are still unknown. In the present study the lymphoproliferative response and the IFN-gamma production by candidiasis patients were evaluated. The stimulation index of mononuclear cell cultures of candidiasis patients stimulated with Candida albicans antigen, PPD and TT were 6 +/- 8, 17 +/- 20 and 65 +/- 30, respectively. The addition of monoclonal antibody anti-IL-10 to Candida albicans antigen stimulated cultures raised the lymphoproliferative response from 735 +/- 415 to 4143 +/- 1746 cpm. The IFN-gamma production by cells of candidiasis patients stimulated with Candida albicans antigen was 162 +/- 345 pg/ml. Candidiasis patients have an impairment in the lymphoproliferative response specific to C. albicans antigen and on IFN-gamma production and the lymphoproliferative response can be partially restored, in vitro, by IL-10 neutralization.
Serviço de Imunologia, Hospital Universitário Prof Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brasil.
Lucas P
LP
Carvalho
Olívia
O
Bacellar
Nilma A
NA
Neves
Edgar M
EM
Carvalho
Amélia R
AR
de Jesus
D000328:Adult; D000946:Antigens, Fungal / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology*; D018929:Cell Culture Techniques; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D012008:Recurrence
D000946:Antigens, Fungal; D016753:Interleukin-10; D007371:Interferon-gamma