Antibody isotype responses in Balb/c mice immunized with the cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi.
In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of all IgG isotypes, mainly IgG3 and IgG1. FRA-immunization elicited only high levels of IgG1.
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, 50670-420 Recife, PE, Brasil.
Valéria R A
VR
Pereira
Virginia Maria B
VM
Lorena
Alinne Fernanda A
AF
Verçosa
Edmilson D
ED
Silva
Antonio G P
AG
Ferreira
Ulisses R
UR
Montarroyos
Ana P Galvão
AP
Silva
Yara M
YM
Gomes
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D015800:Protozoan Proteins; C060967:cytoplasmic antigen, Trypanosoma