Increased gene expression of beta-1,4-galactosyltransferase I in rat injured sciatic nerve.
During neurite outgrowth on basal lamina, cell-surface beta-1,4-galactosyltransferase I (beta-1,4-GalT-I) functions as one of the receptors of laminin by binding to N-linked oligosaccharides on the laminin E8 domain. In the present study, it was revealed that in rat injured sciatic nerves, the expression of beta-1,4-GalT-I mRNA reached its peak 2-3 d after axotomy in both proximal and distal stumps, and decreased thereafter as demonstrated by Northern blot analysis. In situ hybridization revealed that beta-1,4-GalT-I mRNAmainly localized in Schwann cells of the injured nerves. Moreover the Galbeta1-4GlcNAc (N-acetylglucosamine) group mainly localized in Schwann cells of the injured nerves by Ricinus communis agglutinin-I (RCA-I) lectin histochemistry. However, the changes in abundance of the Galbeta1-4GlcNAc group in injured nerves were not consistent with the expression of beta-1, 4-GalT-I mRNA. These findings indicate that beta-1,4-GalT-I might be involved in the regeneration of injured peripheral nerves at the early injury stage.
The Jiangsu Province Key Lab of Neuroregeneration, Nantong Medical College, Nantong 226001, People's Republic of China.
Aiguo
A
Shen
Dan
D
Zhu
Fei
F
Ding
Min
M
Zhu
Xiaosong
X
Gu
Jianxin
J
Gu
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D020439:Growth Cones / Q000201:enzymology / Q000648:ultrastructure; D007797:Laminin / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000201:enzymology* / Q000503:physiopathology; D037121:Plant Lectins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000235:genetics; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000201:enzymology* / Q000503:physiopathology; D015854:Up-Regulation / Q000235:genetics*
D007797:Laminin; D037121:Plant Lectins; D012333:RNA, Messenger; C017707:Ricinus communis agglutinin-1; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I