Mutations in the muscle LIM protein and alpha-actinin-2 genes in dilated cardiomyopathy and endocardial fibroelastosis.
Dilated cardiomyopathy (DCM) is a major cause of morbidity and mortality. Two genes have been identified for the X-linked forms (dystrophin and tafazzin), while mutations in multiple genes cause autosomal dominant DCM. Muscle LIM protein (MLP) is a member of the cysteine-rich protein (CRP) family and has been implicated in both myogenesis and sarcomere assembly. In the latter role, it binds zyxin and alpha-actinin, both of which are involved in actin organization. An MLP-deficient mouse has been described; these mice develop dilated cardiomyopathy and heart failure. Based upon these data, and the recent descriptions of mutations in MLP in patients with DCM or hypertrophic cardiomyopathy, we screened patients for mutations in the MLP and alpha-actinin-2 genes. We identified a patient with DCM and EFE, having a mutation in MLP with the residue lysine 69 substituted by arginine (K69R). This is within a highly conserved region adjacent to the first LIM domain involved in alpha-actinin binding. Analysis in cell culture systems demonstrated that the mutation abolishes the interaction between MLP and alpha-actinin-2 and the cellular localization of MLP was altered. In another individual with DCM, a W4R mutation was identified. However, this mutation did not segregate with disease in this family. In another patient with DCM, a Q9R mutation was identified in alpha-actinin-2. This mutation also disrupted the interaction with MLP and appeared to inhibit alpha-actinin function in cultured cells, in respect to the nuclear localization of actinin and the initiation of cellular differentiation.
Mohapatra
Bhagyalaxmi
B
Department of Pediatrics (Section of Cardiology), Baylor College of Medicine, Houston, TX, USA.
Jimenez
Shinawe
S
Lin
Jiuann Huey
JH
Bowles
Karla R
KR
Coveler
Karen J
KJ
Marx
Joseph G
JG
Chrisco
Michele A
MA
Murphy
Ross T
RT
Lurie
Paul R
PR
Schwartz
Robert J
RJ
Elliott
Perry M
PM
Vatta
Matteo
M
McKenna
William
W
Towbin
Jeffrey A
JA
Bowles
Neil E
NE
D000185:Actinin / Q000235:genetics* / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002311:Cardiomyopathy, Dilated / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016189:Dystrophin / Q000378:metabolism; D004695:Endocardial Fibroelastosis / Q000235:genetics*; D006801:Humans; D060588:LIM Domain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D011485:Protein Binding; D012518:Sarcomeres / Q000235:genetics / Q000378:metabolism
C401401:ACTN2 protein, human; D000199:Actins; C498529:Actn2 protein, mouse; D016189:Dystrophin; D060588:LIM Domain Proteins; D009124:Muscle Proteins; C090114:cysteine and glycine-rich protein 3; D000185:Actinin