Recoveries of rat lymph FA after administration of specific structured 13C-TAG.
The potential of the specific structured TAG MLM [where M = caprylic acid (8:0) and L = linoleic acid (18:2n-6)] is the simultaneous delivery of energy and EFA. Compared with long-chain TAG (LLL), they may be more rapidly hydrolyzed and absorbed. This study examined the lymphatic recoveries of intragastrically administered L*L*L*, M*M*M*, ML*M, and ML*L* (where * = 13C-labeled FA) in rats. Lymph lipids were separated into lipid classes and analyzed by GC combustion isotope ratio MS. The recoveries of lymph TAG 18:2n-6 8 h after administration of L*L*L*, ML*M, and ML*L* were 38.6, 48.4, and 49.1%, respectively, whereas after 24 h the recoveries were approximately 50% in all experimental groups. The exogenous contribution to lymph TAG 18:2n-6 was approximately 80 and 60% at maximum absorption of the specific structured TAG and L*L*L*, respectively, 3-6 h after administration. The tendency toward more rapid recovery of exogenous long-chain FA following administration of specific structured TAG compared with long-chain TAG was probably due to fast hydrolysis. The lymphatic recovery of 8:0 was 2.2% 24 h after administration of M*M*M*. This minor lymphatic recovery of exogenous 8:0 was probably due to low stimulation of chylomicron formation. These results demonstrate tendencies toward faster lymphatic recovery of long-chain FA after administration of specific structured TAG compared with long-chain TAG.
BioCentrum-DTU, Section of Biochemistry & Nutrition, The Technical University of Denmark, 2800 Lyngby, Denmark. bvistisen@ifi.ku.dk
Bodil
B
Vistisen
Huiling
H
Mu
Carl-Erik
CE
Høy
D000818:Animals; D001692:Biological Transport; D002247:Carbon Isotopes; D005227:Fatty Acids / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D008196:Lymph / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*
D002247:Carbon Isotopes; D005227:Fatty Acids; D014280:Triglycerides