Differential expression of PE and PE_PGRS genes in Mycobacterium tuberculosis strains.
In this study, the expression of the Mycobacterium tuberculosis PE, PE_polimorphic GC-rich sequences (PGRS) gene family encoding approximately 99 glycine-rich proteins was assayed by reverse-transcriptase polymerase chain reaction (RT-PCR) in M. tuberculosis H37Rv, Mycobacterium canettii and two clinical isolates of M. tuberculosis. Restriction analyses and sequencing of the RT-PCR products showed that all the strains expressed the PE Rv1172c gene while the PE_PGRS Rv3652 gene was only expressed by one of the M. tuberculosis clinical isolates, and the PE_PGRS Rv0578c was not expressed by M. canettii. It was also determined that the PE_PGRS Rv0278c and Rv0279c were not expressed by any of the studied strains. The data presented in this report show that the PE, PE_PGRS genes are differentially expressed in M. tuberculosis strains during in vitro growth. These findings suggest that PE, PE_PGRS genes may play a role in protein variation between M. tuberculosis strains.
Flores
Javier
J
Departamento de Inmunologi;a, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, México, D.F. 04510, Mexico.
Espitia
Clara
C
D001426:Bacterial Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005998:Glycine / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018076:DNA, Complementary; D012329:RNA, Bacterial; D004262:DNA Restriction Enzymes; D005998:Glycine