[Effects of human fibroblastoid stromal cell line on proliferation of HL-60 cells and expression of VEGF].
To investigate the effects of normal human bone m arrow fibroblastoid stromal cell line (HFCL) on the proliferation of acute myeloid leukemia cell line HL-60 and expression of vascular endothelial growth factor (VEGF), establishing coculture system of leukemia cell line HL-60 and HFCL, growth data was obtained by cell counting. Mitotic index (MI) was observed under Wright-Giemsa staining. Flow cytometry and Western blot were used as assays for cell cycle and expression of proliferating cell nuclear antigen (PCNA) separately. VE GF levels were evaluated by using commercial ELISA kits. The results showed that compared with HL-60 cells without HFCL cells, the proliferation of HL-60 cells in direct contact with HFCL cells and with HFCL cells separated by transwell was inhibited. The MI of HL-60 cells without HFCL cells was highest followed by HL-60 cells separated by transwell and HL-60 cells in direct contact with HFCL cells. The expression of PCNA in HL-60 cells with HFCL cells were lower than HL-60 cells without HFCL cells. Meanwhile, the percentage of HL-60 cells in G1 phase cocultured with HFCL cells was higher than that without HFCL cells while the percentage of Sphase cells was lower. The levels of VEGF in HL-60 cells with HFCL cells were lower than that in HL-60 cells alone. In conclusion, the normal bone marrow fibroblastoid stromal cells inhibited the proliferation of HL-60 cells as well as the expression of VEGF.
Liang
Rong
R
Department of Pathology, Faculty of Preclinical Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
Huang
Gao-Sheng
GS
Chen
Xie-Qun
XQ
Wang
Zhe
Z
Feng
Ji-Liang
JL
Zhang
Wei-Ping
WP
Guo
Ying
Y
Yang
Guo-Yong
GY
D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D018920:Coculture Techniques; D005347:Fibroblasts / Q000502:physiology*; D018922:HL-60 Cells / Q000166:cytology*; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D017154:Stromal Cells / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A