Modulation of tissue immunogenicity by organ culture. Comparison of adult islets and fetal pancreas.
Uncultured mouse islet allografts (BALB/c to CBA) are rejected 2 to 4 weeks after transplantation. Allografts, cultured in 95% O2 and 5% CO2 for 7 days before transplantation, show no sign of rejection up to 3 months post-transplantation. However, the cultured allografts are rejected if the CBA recipient is given an i.v. injection of 10(5) peritoneal cells at the time of transplantation. Organ culture of BALB/c fetal pancreas (16 to 17 days gestation) under the same conditions failed to prevent allograft rejection. The immunogenicity of fetal pancreas is reduced if this tissue is cultured in 95% O2 and 5% CO2 for 17 days before transplantation.
Department of Immunology, John Curtin School of Medical Research, P.O. Box 334, Canberra, Australia, 2601.
C J
CJ
Simeonovic
K M
KM
Bowen
I
I
Kotlarski
K J
KJ
Lafferty
D000818:Animals; D002478:Cells, Cultured; D016332:Fetal Tissue Transplantation / Q000276:immunology / Q000473:pathology; D006085:Graft Survival / Q000276:immunology; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology*; D016381:Islets of Langerhans Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques / Q000379:methods*; D014185:Transplantation, Isogeneic / Q000276:immunology* / Q000473:pathology