Changes in some enzymes of microbodies and plastid development in excised radish cotyledons: effect of narciclasine.
Narciclasine (NCS), isolated from mucilage of Narcissus bulb, showed inhibitory effects on growth and plastid development of excised radish cotyledons. NCS (0.1 mumol/L) started to show inhibitory effects on isocitrate lyase and hydroxypyruvate reductase activities after 24 h incubation in light. When NCS concentration was increased to 10 mumol/L, the activities of both enzymes are completely inhibited. From ultrastructural studies, NCS markedly prevented the degradation of protein bodies and lipid bodies, as well as chloroplast formation of excised radish cotyledons. There was only little degradation of protein and lipid bodies, and almost no chloroplast formation in the excised radish cotyledon treated with 1 mumol/L NCS. Therefore, our results provide clear evidence that NCS inhibited the transition of glyoxysomes and peroxisomes, and chloroplast development.
School of Life Sciences, State Key Laboratory of Arid Agroecology, Lanzhou 730000, Lanzhou University, P.R. China. yrbi@lzu.edu.cn, zhanglx6@hotmail.com
Yurong
Y
Bi
Jinkui
J
Guo
Lixin
L
Zhang
Yumshing
Y
Wong
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D047151:Amaryllidaceae Alkaloids; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D050537:Hydroxypyruvate Reductase; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology; D008854:Microscopy, Electron; D031427:Narcissus / Q000737:chemistry; D010617:Phenanthridines; D010937:Plant Growth Regulators / Q000302:isolation & purification / Q000494:pharmacology; D018087:Plastids / Q000187:drug effects / Q000201:enzymology; D031224:Raphanus / Q000187:drug effects* / Q000201:enzymology* / Q000648:ultrastructure
D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D004791:Enzyme Inhibitors; D010617:Phenanthridines; D010937:Plant Growth Regulators; C010753:narciclasine; D000429:Alcohol Oxidoreductases; D050537:Hydroxypyruvate Reductase; D007522:Isocitrate Lyase