Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells: a global analysis of host gene regulation during infection, using a differential display approach.
Autographa californica nucleopolyhedrovirus (AcMNPV), the type member of the virus family Baculoviridae, infects pest insects and has been the subject of many studies for its development as a biopesticide. It is also the virus upon which most of the commercial baculovirus protein expression systems are based. AcMNPV infection of cultured host Spodoptera frugiperda (Sf9) cells can induce a number of alterations of host cell properties including altering the cellular cytoskeleton, an arrest of the cell cycle in G(2)/M, and the global shutoff of host protein translation. Additionally, several cellular transcripts have been shown to be down-regulated following AcMNPV infection. In this study, we take a differential display approach to address whether a global down-regulation of Sf9 host transcripts occurs at late times of infection. Additionally, we also use this approach to search for host mRNAs which are up-regulated at early times of infection, and may be important for facilitating baculovirus infection. From these experiments we can confirm a global down-regulation of Sf9 mRNA levels at late times of infection. We also found that up-regulation of individual host gene RNA levels at early times of infection did not occur frequently. One host transcript which was found to be transiently up-regulated as a result of AcMNPV infection was an Sf9 Hsc70 gene. Hsc70 proteins have been shown to play a vital role in the life-cycle of other large DNA viruses, which suggests that this protein is also important for baculovirus infection.
Department of Biological Sciences, SAF Building, South Kensington Campus, Imperial College, London SW7 2AZ, UK.
Isabelle
I
Nobiron
Department of Biological Sciences, SAF Building, South Kensington Campus, Imperial College, London SW7 2AZ, UK.
David R
DR
O'Reilly
Department of Biological Sciences, SAF Building, South Kensington Campus, Imperial College, London SW7 2AZ, UK.
Julie A
JA
Olszewski
D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D018411:Spodoptera / Q000821:virology*
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins