Localization of ligand-binding sites on human C1q globular head region using recombinant globular head fragments and single-chain antibodies.
As a charge pattern recognition molecule, human C1q can bind a range of immunoglobulin and non-immunoglobulin ligands via its carboxy-terminal globular domain and activate the classical complement pathway. Each globular domain has a heterotrimeric organization, composed of the carboxy-terminal halves of one A (ghA), one B (ghB), and one C (ghC) chain. Recently, we have found that the recombinant forms of individual ghA, ghB and ghC bind differentially to IgG, IgM, gp41 peptide 601-613 of human immunodeficiency virus-1 (HIV-1), gp21 peptide 400-429 of human T cell lymphotrophic virus-I (HTLV-I), beta-amyloid peptide, and apoptotic cells, suggesting a modular organization of the globular domain. This paper examines the interaction of ghA, ghB and ghC with two known C1q ligands: Klebsiella pneumoniae porin OmpK36 and salivary agglutinin. In addition, we have used a panel of recombinant single-chain antibodies (scFv) specific for ghA, ghB and ghC in order to map sites on the heterotrimeric globular domain which are likely to interact with IgG1, IgG3, IgM, OmpK36, salivary agglutinin and gp41 loop peptide. The combined use of recombinant ghA, ghB, ghC and single-chain antibodies has revealed at least three ligand-binding sites on the globular domain of C1q: one is IgG- and OmpK36-specific, the second (IgM-binding site) is most likely overlapping with IgG/OmpK36 binding site, and the third (the gp41-binding site) seems to be located at the junction between the collagen and globular domains.
Kojouharova
Mihaela S
MS
Department of Biochemistry, Sofia University, St. Kliment Ohridski, 8 Dragan Tzankov Str, Sofia 1164, Bulgaria.
Tsacheva
Ivanka G
IG
Tchorbadjieva
Magdalena I
MI
Reid
Kenneth B M
KB
Kishore
Uday
U
D000373:Agglutinins / Q000378:metabolism; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001665:Binding Sites; D015922:Complement C1q / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015700:HIV Envelope Protein gp41 / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000737:chemistry; D008024:Ligands; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D018272:Porins / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012469:Salivary Glands / Q000737:chemistry
D000373:Agglutinins; D001425:Bacterial Outer Membrane Proteins; D015700:HIV Envelope Protein gp41; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008024:Ligands; D010446:Peptide Fragments; D018272:Porins; D011994:Recombinant Proteins; D015922:Complement C1q