pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein.
We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G+C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were homologous to those of other bacterial plasmid Rep proteins, the degree of similarity was very low. A deletion analysis showed that the Rep protein was not required for the replication of pHP489 in its H. pylori host, but the host replication machinery was needed.
Song
Jae-Young
JY
Department of Microbiology, Gyeongsang National University College of Medicine, 90 Chiram-dong, Jinju, Gyeongsangnam-do 660-751, Republic of Korea.
Park
Seong-Gyu
SG
Kang
Hyung-Lyun
HL
Lee
Woo-Kon
WK
Cho
Myung-Je
MJ
Park
Jeong-Uck
JU
Baik
Seung-Chul
SC
Youn
Hee-Shang
HS
Ko
Gyung-Hyuck
GH
Rhee
Kwang-Ho
KH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; C481641:RepH protein, Helicobacter pylori