Effects of (-)-menthol on the distribution and metabolism of 2-aminofluorene in various tissues of Sprague-Dawley rats.
The purpose of the present study was to examine whether or not (-)-Menthol affects the distribution and metabolism of 2-aminofluorene (AF) in Sprague-Dawley rats. The AF, acetylated AF and AF metabolites were determined by using high performance liquid chromatography. AF was administered orally alone, with (-)-Menthol at the same time, and after 24-hour (-)-Menthol pretreatment and then urine, stool, blood and tissues from liver, kidneys, stomach, colon and bladder were collected and assayed for AF and its metabolites. Compared to the control group, (-)-Menthol caused an increase of the metabolites excreted in the urine and stool. The major metabolite excreted both in the urine and in the stool was 9-OH-AAF. The liver was the major metabolism center and the major residual metabolite of AF in the liver was 9-OH-AAF. When AF was given for 24 hours with (-)-Menthol to SD rats, the rate of carcinogen acetylation is decreased in bladder, blood, colon, kidney and liver tissues.
School of Chinese Medicine, China Medical University, No 91, Hsueh-Shih Road, Taichung 400, Taiwan, ROC.
Jing-Pin
JP
Lin
Kwork-Chui
KC
Cheng
Guang-Wei
GW
Chen
Mei-Due
MD
Yang
Tsan-Hung
TH
Chiu
Jing-Gung
JG
Chung
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004347:Drug Interactions; D005449:Fluorenes / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008610:Menthol / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D014018:Tissue Distribution / Q000187:drug effects
D002273:Carcinogens; D005449:Fluorenes; D008610:Menthol; C012177:2-aminofluorene