Cross-reactive H1N1 antibody responses to a live attenuated influenza vaccine in children: implication for selection of vaccine strains.
Cross-reactive antibody responses of 3 trivalent, live attenuated intranasal influenza vaccine (FluMist) formulations containing 3 different H1N1 strains (A/Texas/36/91, A/Shenzhen/227/95, and A/Beijing/262/95) were evaluated in initially seronegative children. FluMist containing A/Shenzhen/227/95 was more likely to induce cross-reactive hemagglutination inhibition (HAI) antibody against A/Texas/36/91 than against A/Beijing/262/95, and FluMist containing A/Beijing/262/95 induced low levels of cross-reactive HAI antibody against A/Shenzhen/227/95 and A/New Caledonia/20/99. The observed differences in HAI cross-reactivity seem to be partly related to the number of amino acid (aa) differences on the hemagglutinin 1 domain (328 aa residues) rather than the hemagglutinin protein (550 aa residues).
Lee
Min-Shi
MS
MedImmune Vaccines, Mountain View, California 94043, USA. mslee007@us.sina.com.
Yang
Chin-Fen
CF
D000281:Administration, Intranasal; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D002675:Child, Preschool; D003429:Cross Reactions / Q000235:genetics / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007223:Infant; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control; D009975:Orthomyxoviridae / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated