Stimulus-induced patterns of bioelectric activity in human neocortical tissue recorded by a voltage sensitive dye.
Stimulus-induced pattern of bioelectric activity in human neocortical tissue was investigated by use of the voltage sensitive dye RH795 and a fast optical recording system. During control conditions stimulation of layer I evoked activity predominantly in supragranular layers showing a spatial extent of up to 3000 microm along layer III. Stimulation in white matter evoked distinct activity in infragranular layers with a spatial extent of up to 3000 microm measured along layer V. The mean amplitude of optical signals close to the stimulated sites in layer I and white matter determined 25 ms following the stimulus, decreased by 50% at a lateral distance of approximately 900 microm and 1200 microm, respectively. Velocity of spread along the vertical stimulation axis reached 0.24 m/s in the supragranular layers (layers I to III) and then decreased to 0.09 m/s following layer I activation; stimulation of white matter induced a velocity of spread in layer V of 0.38 m/s, which slowed down to 0.12 m/s when passing the lower border of lamina IV. The horizontal velocities of spread determined from the stimulation site to a lateral distance of 500 microm reached 0.26-0.28 m/s and 0.28-0.35 m/s for layer I and white matter stimulation, respectively. At larger distances velocity of spread decreased. Increased excitability (Mg(2+)-free solution) had no significant effect on the spatio-temporal distribution of evoked activity as compared with control conditions. There were also no obvious differences between the results obtained in slices, which generated spontaneously sharp waves and those which were not spontaneously active. About 30% of the slices (n=7) displayed a greatly different response pattern, which seemed not to be related in a simple way to the stimulation as was the case in the majority of the investigated slices. The activity pattern of those slices appeared atypical in regard to their deviations of the vertical and horizontal extent of activity, to their reduced spatial extent of activity during increased excitability, to their layer-related distribution of activity, and to the appearance of afterdischarges.Concluding, in 30% of the human temporal lobe slices atypical activity pattern occurred which obviously reflect intrinsic epileptiform properties of the resected tissue. The majority of slices showed stereotyped activity pattern without evidence for increased excitability.
Institut für Physiologie, Westfälische Wilhelms-Universität, Robert-Koch-Strasse 27a, D-48149 Münster, Germany. straub@uni-muenster.de
H
H
Straub
U
U
Kuhnt
J-M
JM
Höhling
R
R
Köhling
A
A
Gorji
D
D
Kuhlmann
I
I
Tuxhorn
A
A
Ebner
P
P
Wolf
H-W
HW
Pannek
R
R
Lahl
E-J
EJ
Speckmann
D000293:Adolescent; D000328:Adult; D001931:Brain Mapping; D002648:Child; D002675:Child, Preschool; D003952:Diagnostic Imaging / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D004553:Electric Conductivity; D004558:Electric Stimulation; D004594:Electrophysiology; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery; D005071:Evoked Potentials / Q000502:physiology*; D005260:Female; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019579:Neocortex / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D011930:Reaction Time; D013343:Styrenes / Q000493:pharmacokinetics; D013997:Time Factors
D005456:Fluorescent Dyes; C096453:RH 795; D013343:Styrenes; D008274:Magnesium