Enhanced sensitivity and spatial resolution for in vivo imaging with low-level light-emitting probes by use of biocompatible chemical agents.
We describe a technique that uses biocompatible chemical agents to enhance both the sensitivity and the resolution of in vivo imaging with low-level light-emitting probes. We demonstrate experimentally, with chemiluminescence (CL) imaging in vitro as an example, that the detected intensity of CL from treated 3-mm-thick skin tissue is approximately fivefold stronger than that from untreated skin. The spatial resolution correspondingly increases approximately threefold.
He
Yonghong
Y
Biophotonics Laboratory, Institute of BioScience and Technology, Cranfield University at Silsoe, Silsoe, Bedfordshire MK45 4DT, UK.
Wang
Ruikang K
RK
Xing
Da
D
D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000494:pharmacology*; D003952:Diagnostic Imaging; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology; D008027:Light; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009995:Osmosis; D016877:Oxidants / Q000494:pharmacology; D012867:Skin / Q000033:anatomy & histology* / Q000378:metabolism; D013552:Swine
D001672:Biocompatible Materials; D007202:Indicators and Reagents; D016877:Oxidants; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase