Trapping of 2,7-dichlorodibenzo-p-dioxin in aqueous solution by enzymatic reaction of fungal manganese peroxidase in the presence of polyunsaturated fatty acids.
In the presence of polyunsaturated fatty acids, including cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), 2,7-dichlorodibenzo-p-dioxin (DCDD) was treated with manganese peroxidase (MnP) from white rot basidiomycete Phanerochaete sordida YK-624. After incubation with MnP, DCDD could not be extracted from the reaction mixture with n-hexane and was trapped in the water layer. DCDD was released by alkalification of the water layer. DCDD was also trapped after treatment with lipoxidase, which produces hydroperoxides from unsaturated lipids. The amounts of thiobarbituric acid-reactive substances produced in the MnP reactions with three highly unsaturated fatty acids were higher than the amounts produced with three fatty acids with a lower degree of unsaturation. These results suggest that a DCDD-trapping compound may be produced by peroxidation of the polyunsaturated fatty acids.
Harazono
Koichi
K
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan. harazono-koichi@aist.go.jp
Watanabe
Yoshio
Y
Fukatsu
Takema
T
Kurane
Ryuichiro
R
D005591:Chemical Fractionation; D004147:Dioxins / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism; D006586:Hexanes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D013858:Thiobarbiturates / Q000737:chemistry
D004147:Dioxins; D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D006586:Hexanes; D013858:Thiobarbiturates; C026385:n-hexane; C000925:2,7-dichlorodibenzo-4-dioxin; D010544:Peroxidases; C051129:manganese peroxidase; D008084:Lipoxygenase; C029684:thiobarbituric acid