Glycogen synthase kinase-3beta phosphorylates synphilin-1 in vitro.
alpha-Synuclein is known to be a major component of Lewy bodies and glial cytoplasmic inclusions in the brains of patients with alpha-synucleinopathies. Synphilin-1, an alpha-synuclein-associated protein, is also present in these inclusions. However, little is known about the post-translational modifications of synphilin-1. In the present study, it is reported that synphilin-1 is phosphorylated by glycogen synthase kinase-3beta in vitro. It is well known that protein phosphorylation is involved in various physiological phenomena, including signal transduction and protein degradation. Therefore, phosphorylation of synphilin-1 may play an important role in the function of this protein in the brain.
Department of Neuropathology, Hirosaki University School of Medicine, Hirosaki, Japan.
Kunikazu
K
Tanji
Tsutomu
T
Toki
Wakako
W
Tamo
Tadaatsu
T
Imaizumi
Tomoh
T
Matsumiya
Fumiaki
F
Mori
Hitoshi
H
Takahashi
Kei
K
Satoh
Koichi
K
Wakabayashi
D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D014162:Transfection
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C118958:SNCAIP protein, human; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3