Peach (Prunus persica L.) fruit growth and ripening: transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp.
Transcript levels and activities of the polyamine biosynthetic enzymes arginine decarboxylase (ADC, EC 4.1.1.19), ornithine decarboxylase (ODC, EC 4.1.1.17) and S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.21), as well as free polyamine titres, were analysed throughout the four growth stages S1-S4 leading up to ripening in the mesocarp from peach fruit (Prunus persica L. Batsch cv. Redhaven) grown under field conditions. SAMDC mRNA, which was northern analysed by using a PCR-generated homologous SAMDC probe, and ADC mRNA levels appeared quite stable during fruit development, while ODC transcript accumulation showed a discontinuous trend. The pattern of transcript levels during growth did not correlate with that of the relative enzyme activity, which instead correlated well with free polyamine levels. Both exhibited maximum levels in S1 and a smaller peak in S3. The behaviour of the polyamine biosynthetic machinery is discussed in relation to the different cell growth rates occurring during fruit development.
Dipartimento di Biologia e.s., University of Bologna, Via Irnerio 42, I-40126 Bologna, Italy.
Vanina
V
Ziosi
Sonia
S
Scaramagli
Anna M
AM
Bregoli
Stefania
S
Biondi
Patrizia
P
Torrigiani
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics / Q000378:metabolism; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D018514:Plant Structures; D027861:Prunus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011700:Putrescine / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism
D015317:Biogenic Polyamines; D012333:RNA, Messenger; D018749:RNA, Plant; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; C019722:arginine decarboxylase; D012437:Adenosylmethionine Decarboxylase; D011700:Putrescine