Purification and identification of antimicrobial sesquiterpene lactones from yacon (Smallanthus sonchifolius) leaves.
The extraction of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson; Asteraceae] leaves and chromatographic separation yielded two new antibacterial melampolide-type sesquiterpene lactones, 8beta-tigloyloxymelampolid-14-oic acid methyl ester and 8beta-methacryloyloxymelampolid-14-oic acid methyl ester, as well as the four known melampolides, sonchifolin, uvedalin, enhydrin and fluctuanin. The newly identified compound, 8beta-methacryloyloxymelampolid-14-oic acid methyl ester, exhibited potent antimicrobial activity against Bacillus subtilis and Pyricularia oryzae, while 8beta-tigloyloxymelampolid-14-oic acid methyl ester showed lower activity. Fluctuanin exhibited the strongest antibacterial activity against B. subtilis among these six sesquiterpene lactones.
Laboratory of Phytochemical Ecology, College of Agriculture, Ibaraki University, Chuo, Ami, Ibaraki, Japan.
Fengqiu
F
Lin
Morifumi
M
Hasegawa
Osamu
O
Kodama
D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000302:isolation & purification / Q000494:pharmacology*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D000890:Anti-Infective Agents; D007783:Lactones; D012717:Sesquiterpenes