The relevance of cell size on ultrasound-induced hemolysis in mouse and human blood in vitro.
This paper describes a further test of the hypothesis that cell size is an important physical parameter in ultrasound (US)-induced hemolysis, that is, the larger the cell the greater the potential for sonolysis by a cavitational mechanism. Mouse (M) and human (Hu) erythrocytes in vitro were used; their mean corpuscular volumes were 49.0 and 89.5 fL, respectively. At a US exposure in vitro in the presence of Albunex that yielded an average of 36.8% hemolysis for M blood, the Hu blood yielded an average of 54.0% hemolysis. The data supported the hypothesis. This paper also briefly discusses the difficulty of extrapolating sonolytic in vitro results to those derived in vivo.
Department of Obstetrics and Gynecology, School of Medicine and Dentistry, The University of Rochester, Rochester, NY 14642-0001, USA. Morton_Miller@urmc.rochester.edu
Morton W
MW
Miller
Linda F
LF
Battaglia
D000818:Animals; D048429:Cell Size; D003287:Contrast Media; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D014463:Ultrasonography
D003287:Contrast Media