[Activity of dsRNA-dependent protein kinase in rat lymphoid cells under the effect of X-ray irradiation].
The activity of dsRNA-dependent protein kinase, which is the key enzyme of the interferon signal system, was studied in the rat spleen and thymus lymphocytes under the influence of X-ray irradiation at 0.5 and 1 Gy doses and interferon inducers administration. An increase of the enzyme activity was established in the presence of FGA, concanavaline A, poly(I).poly(C) in vitro. The effect is intensified under the irradiation by 0.5 Gy dose. The protein kinase activity in lymphocytes is amplified in proportion to poly(I).poly(C) concentration, that was most pronounced in the irradiated animals. The comparative analysis of the action of interferon inducers on the dsRNA-dependent protein kinase activity was carried out. Two biological systems were used: in vivo (when the preparations were injected to the experimental animals) and in vivo (under the preincubation of isolated lymphocytes with the inducers). It was shown that the combined action of radiation and interferon inducers causes the stimulation of dsRNA-dependent protein kinase activity.
Mykhaĭlyk
I V
IV
Kyiv Taras Shevchenko National University, Ukraine. imihalik@yahoo.com
Prokopova
K V
KV
Ostapchenko
L I
LI
Kucherenko
M Ie
MIe
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology / Q000528:radiation effects*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D011066:Poly C / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology; D011494:Protein Kinases / Q000528:radiation effects*; D012330:RNA, Double-Stranded / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects; D014965:X-Rays
D007369:Interferon Inducers; D010835:Phytohemagglutinins; D012330:RNA, Double-Stranded; D003208:Concanavalin A; D011069:Poly I; D011066:Poly C; D007372:Interferons; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase