[Establishment of malignant progression associated gene expression profiles in human brain glioma].
OBJECTIVE
To establish malignant progression associated gene expression profiles in human brain glioma.


METHODS
The primary (WHO grade II), recurrent (WHO grade III) and re-recurrent (WHO grade IV) glioma specimens were sequentially collected from one single patient. Gene expression of different tumor specimens and normal brain tissue of the same patient was compared by microarrary techniques.


RESULTS
197 differentially expressed genes with differential ratio > or = 3 were observed when compared with normal brain tissue. When the specimens (3 tumor, 1 normal brain) were paired with each other, 7 groups containing 489 genes (upregulated 193, downregulated 296) were observed. According to the descending frequency of the 109 genes with known function, they were the genes associated with development, metabolism, differentiation, signal transduction, DNA binding transcription, cellular receptor, immunity, ion-channel transportation, protein translation, cell backbone motion, stress, protooncogene and anti-oncogene and cell apoptosis, respectively.


CONCLUSIONS
From the 197 differentially expressed genes found in one glioma patient experiencing tumor malignant progression, 17 genes screened out by bioinformatics assay, may offer valuable information on molecular mechanisms on genesis and malignant progression of glioma.
Neurosurgical Department and Brain Tumor Research Laboratory, Second Affiliated Hospital, Suzhou University, Suzhou 215004, China.
Qiang
Q
Huang
Jun
J
Dong
Ai-dong
AD
Wang
Nai-yuan
NY
Shao
Ji-yong
JY
Sun
Xiao-nan
XN
Li
Qing
Q
Lan
Geng-xi
GX
Hu
D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D020869:Gene Expression Profiling; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis