Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor.
Syringolide elicitors produced by bacteria expressing Pseudomonas syringae pv. glycinea avirulence gene D (avrD) induce hypersensitive cell death (HCD) only in soybean (Glycine max [L.] Merr.) plants carrying the Rpg4 disease resistance gene. Employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar Harosoy (Rpg4) treated with syringolides. Several genes for isolated fragments were induced by syringolides in an rpg4 cultivar Acme as well as in Harosoy; however, the genes for seven fragments designated as SIH (for syringolide-induced/ HCD associated) were induced exclusively or strongly in Harosoy. cDNA clones for SIH genes were obtained from a cDNA library of Harosoy treated with syringolide. Several sequences are homologous to proteins associated with plant defense responses. The SIH genes did not respond to a non-specific beta-glucan elicitor, which induces phytoalexin accumulation but not HCD, suggesting that the induction of the SIH genes is specific for the syringolide-Harosoy interaction. HCD and the induction of SIH genes by syringolides were independent of H(2)O(2). On the other hand, Ca(2+) was required for HCD and the induction of some SIH genes. These results suggest that the induction of SIH genes by syringolides could be activated through the syringolide-specific signaling pathway and the SIH gene products may play an important role(s) in the processes of HCD induced by syringolides.
Division of Biological Sciences, Graduate School of Science, Hokkaido University, 060-0810 Sapporo, Japan.
Takuya
T
Hagihara
Masaru
M
Hashi
Yoji
Y
Takeuchi
Naoto
N
Yamaoka
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D015152:Blotting, Northern; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D007700:Kinetics; D010940:Plant Proteins / Q000235:genetics*; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
D001426:Bacterial Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins