The Wilms' tumour suppressor protein, WT1, undergoes CRM1-independent nucleocytoplasmic shuttling.
The Wilms' tumour suppressor gene (WT1) encodes a zinc finger-containing nuclear protein essential for kidney and urogenital development. Initially considered a transcription factor, there is mounting evidence that WT1 has a role in post-transcriptional processing. Using the interspecies heterokaryon assay, we have demonstrated that WT1 can undergo nucleocytoplasmic shuttling. We have also mapped the region responsible for nuclear export to residues 182-324. Our data add further complexity to the role of WT1 in transcriptional and post-transcriptional regulation.
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Qld 4072, Australia. p.vajjhala@imb.uq.edu.au
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Vajjhala
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Macmillan
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Gonda
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Little
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D028884:Karyopherins; D051379:Mice; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D012323:RNA Processing, Post-Transcriptional; D018160:Receptors, Cytoplasmic and Nuclear; D014158:Transcription, Genetic; D014162:Transfection; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism*; D000097606:Exportin 1 Protein
D018076:DNA, Complementary; D028884:Karyopherins; D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear; D025721:WT1 Proteins; D000097606:Exportin 1 Protein