Generation of antibodies against prion protein in wild-type mice via helix 1 peptide immunization.
We present here the development of antibodies against prion protein in BALB/C mice using as antigen human helix 1 of PrP. This sequence is suggested to be involved in protein pathological conformational changes, and is distinguished from that of mice by one amino acid. The immune tolerance to an 'almost-self' epitope and the poor immunogenicity of short peptides was overcome by using Multiple Antigen Peptide displaying eight copies of helix 1. The generated antibodies recognize the whole prion protein with a high binding constant and the established protocol may lead to an active immunization towards therapeutics of prion disease.
Arbel
Michal
M
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
Lavie
Vered
V
Solomon
Beka
B
D000818:Animals; D000910:Antibodies, Heterophile / Q000096:biosynthesis* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015478:Antigens, Heterophile / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D002417:Cattle; D045744:Cell Line, Tumor; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000276:immunology* / Q000378:metabolism; D018621:PrPC Proteins / Q000276:immunology* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011817:Rabbits; D012756:Sheep; D014611:Vaccination / Q000379:methods
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D018621:PrPC Proteins