Characterization of hydrophobic peptides by atmospheric pressure photoionization-mass spectrometry and tandem mass spectrometry.
The use of photoionization at atmospheric pressure shows great potential for the mass analysis of large apolar or hydrophobic peptides. Mass spectra that were obtained using this technique showed mainly singly charged ions. While polar peptides spectra do not produce fragment ions, others lead to B-type or C-type in-source fragmentation. These dissociation reactions, which could involve electron capture dissociation processes in the case of the C-type ions, are observed for hydrophobic peptides. Both the compatibility of this ionization mode with reversed- or normal-phase liquid chromatographic separation and its sensitivity allow liquid chromatography coupling to both mass spectrometry and tandem mass spectrometry for the analyses of hydrophobic peptide mixtures. Atmospheric pressure photoionization seems to be an interesting alternative method to study hydrophobic peptides that are not easily ionizable by more classical ionization techniques such as electrospray ionization and matrix-assisted laser desorption/ionization.
Delobel
Arnaud
A
Institut de Chimie des Substances Naturelles, CNRS, avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France.
Halgand
Frédéric
F
Laffranchise-Gosse
Barbara
B
Snijders
Henri
H
Laprévote
Olivier
O
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000737:chemistry; D001274:Atmospheric Pressure; D001920:Bradykinin / Q000737:chemistry; D002853:Chromatography, Liquid; D006096:Gramicidin / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D010782:Photolysis; D017785:Photons; D011092:Polyethylene Glycols / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014466:Ultraviolet Rays
D007477:Ions; D010455:Peptides; D000804:Angiotensin II; D006096:Gramicidin; D011092:Polyethylene Glycols; D001920:Bradykinin