Experimental proof of contamination of blood components by (1-->3)-beta-D-glucan caused by filtration with cellulose filters in the manufacturing process.
The level of (1-->3)-beta-D-glucan in blood is a diagnostic index of fungal infection because it is released from the fungal cell wall. However, high levels of plasma (1-->3)-beta-D-glucan in patients administered blood components may give false positive results. High levels of (1-->3)-beta-D-glucan have been detected in blood components. We suspected that (1-->3)-beta-D-glucan from cellulose filters had been eluted into blood components by filtration in the manufacturing process. To investigate the contamination of blood components by (1-->3)-beta-D-glucan from cellulose filters, in vitro experiments were performed by using six cellulose filters and a nylon filter. Human serum albumin (HSA) solution (100 ml) was flowed through each filter after rinsing with 100 ml of distilled water, and (1-->3)-beta-D-glucan in each fraction was determined by Fungitec G test MK. The concentration of (1-->3)-beta-D-glucan eluted from cellulose filters in 100-ml distilled water fractions ranged from 6 to 207 pg/ml, and that of HSA fractions ranged from 33 to 20,784 pg/ml. These data showed that remarkably higher (1-->3)-beta-D-glucan levels were detected in HSA fractions flowed through cellulose filters in spite of advance rinsing with 100 ml of distilled water. In the case of a nylon filter, (1-->3)-beta-D-glucan was not eluted in either fraction. These results indicate that (1-->3)-beta-D-glucan contamination in blood components is caused by filtration with cellulose filters in the manufacturing process.
Department of Food Science, Faculty of Technology, University of East Asia, 2-1 Ichinomiya-Gakuencho, Shimonoseki, Yamaguchi 751-8503, Japan. nagasawa@po.cc.toua-u.ac.jp
Koichi
K
Nagasawa
Tatsuyuki
T
Yano
Go
G
Kitabayashi
Hiroaki
H
Morimoto
Yuji
Y
Yamada
Atsushi
A
Ohata
Makoto
M
Usami
Takashi
T
Horiuchi
D001774:Blood Chemical Analysis; D002482:Cellulose / Q000009:adverse effects*; D005189:False Positive Reactions; D005374:Filtration / Q000295:instrumentation*; D005936:Glucans / Q000032:analysis / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D008420:Manufactured Materials / Q000009:adverse effects; D008567:Membranes, Artificial; D009181:Mycoses / Q000097:blood*; D047071:beta-Glucans
D005936:Glucans; D008567:Membranes, Artificial; D047071:beta-Glucans; D002482:Cellulose; C033363:beta-1,3-glucan