Strain PM2, a novel methylotrophic fluorescent Pseudomonas sp.
A novel bacterial strain, PM2, capable of growing on methanol, was isolated in alkaline conditions from a soil inoculum. This bacterium was characterized at the physiological, biochemical and molecular level. Based on biochemical and molecular data strain PM2 was classified as a novel member of the group of fluorescent pseudomonads. Evidence for the presence of a pyrroloquinoline quinone (PQQ)-linked alcohol dehydrogenase in this organism is presented. Strain PM2 is, to our knowledge, the first example of a methylotrophic Pseudomonas to be characterized in detail. This novel type of metabolism in Pseudomonas broadens even further the metabolic versatility for which this genus is renowned.
Cellular and Applied Microbiology group, IBMC, University of Porto, R. do Campo Alegre, 823, 4150-180, Porto, Portugal.
Catarina C
CC
Pacheco
João F
JF
Passos
Pedro
P
Moradas-Ferreira
Paolo
P
De Marco
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D005453:Fluorescence; D000432:Methanol / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
D000429:Alcohol Oxidoreductases; C048312:polyvinyl-alcohol dehydrogenase (acceptor); C048311:polyvinyl alcohol oxidase; D000432:Methanol