Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins.
Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), a recently described disease of young pigs. Since no PCV2 vaccine was available so far, we have developed a specific PCV2 vaccine candidate. The Orf1-encoded replication protein and Orf2-encoded capsid protein of PCV2 were expressed and detected in either mammalian or insect expression systems. In a first trial, Orf2 protein was found to be a major immunogen, inducing protection in a prime-boost protocol; the piglets received a first injection with plasmids directing Orf2 protein and granulocyte-macrophage colony-stimulating factor (GM-CSF) expression, followed by a second injection, a fortnight later, associated with baculovirus-expressed Orf2 protein. As evaluated by growth parameters, clinical signs (fever), seroconversion, the pigs were protected against a PCV2 challenge after vaccination. In a second trial, protection induced by a subunit vaccine was even better than the one induced by DNA vaccine, since PCV2 replication was completely inhibited.
Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Ploufragan, Unité Génétique Virale et Biosécurité, BP 53, Zoopôle Les Croix, Fr 22440 Ploufragan, France. p.blanchard@ploufragan.afssa.fr
P
P
Blanchard
D
D
Mahé
R
R
Cariolet
A
A
Keranflec'h
M A
MA
Baudouard
P
P
Cordioli
E
E
Albina
A
A
Jestin
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D018173:Circoviridae Infections / Q000503:physiopathology / Q000517:prevention & control* / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005334:Fever / Q000503:physiopathology / Q000517:prevention & control; D007117:Immunization, Secondary; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D015430:Weight Gain / Q000502:physiology
D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines