Yersinia enterocolitica O:3 isolated from patients with or without reactive arthritis induces polyclonal activation of B cells and autoantibody production in vivo.
The mechanisms by which arthritis-provoking pathogens such as Yersinia enterocolitica interact with the human immune system to produce inflammatory synovitis are not well known. One of the immunomodulating mechanisms used against these pathogens is the polyclonal activation of lymphocytes. In this study, we investigated the extent of the B-lymphocyte activation induced in mice by a strain of Y. enterocolitica O:3 (FCF 526) isolated from a patient with arthritis, and compared it with two other strains, a virulent one (FCF 397[+]) isolated from a patient without arthritis and its plasmidless isogenic pair (FCF397[-]). Also we investigated the production of autoantibodies in mice infected with these different strains. SPF Swiss mice were infected intravenously with a suspension of Y. enterocolitica. Spleen cells were taken on days 7, 14, 21 and 28 after infection and the number of cells secreting nonspecific and specific antibodies of IgG1, IgG2a, IgG2b, IgG3, IgM and IgA isotypes were determined by the ELISPOT technique. The presence of autoantibodies in mouse serum was investigated by the dot-blot assay. The pattern of infection of the three bacterial strains were almost the same. We observed a general increase in the number of nonspecific Ig-secreting cells with all three strains, and the greatest increases observed were in the IgG2a and IgG3 isotypes. Only a small fraction of the immunoglobulins detected were antibacterial, suggesting that the rest resulted from polyclonal B cell activation. The strain isolated from the patient with arthritis (FCF526) induced the greatest production of autoantibodies, coinciding with the period in which the greatest activation of nonspecific B lymphocytes was seen. There were no signs of arthritis or inflammation in the joints of the infected animals. Based on our results, we were unable to determine whether there is an association between the arthritogenic capability of Y. enterocolitica and polyclonal activation of B cells.
Silva
Eloisa Elena Cangiani
EE
Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP, Araraquara, SP, Brazil.
Ramos
Orivaldo Pereira
OP
Bauab
Taís Maria
TM
Falcão
Deise Pasetto
DP
de Medeiros
Beatriz Maria Machado
BM
D000199:Actins / Q000276:immunology; D000818:Animals; D016918:Arthritis, Reactive / Q000276:immunology* / Q000382:microbiology*; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008099:Liver / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D051379:Mice; D009218:Myosins / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D015009:Yersinia Infections / Q000276:immunology* / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification
D000199:Actins; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009218:Myosins