[Efficient production of wheat alien translocation lines and characterization by molecular cytogenetics].
The Triticum aestivum-Leymus mollis and T. aestivum-Thinopyrum intermedium translocation lines were induced by gametocidal chromosome 3C derived from Aegilops triuncialis and gamma-ray irradiated pollens of a T. aestivum-Th. intermedium addition line TAI-14 with a lower dosage (10Gy), respectively. By genomic in situ hybridization (GISH) analysis, three T. aestivum-L. mollis translocation lines (WM-10, WM-43 and WM-47) and three deletion lines (WM-18, WM-43 and WM-44) were selected from 59 F2 plants derived from a cross combination of T. aestivum-L. mollis substitution line M8724-8-13 x T. aestivum-Ae. triunicalis 3C chromosome addition line. The frequency of translocation lines produced and total frequency of chromosome structural variation occurred were 5.08% and 8.47%, respectively. Two of the three translocation lines, WM-10 and WM-43 all were heterozygous translocation lines carrying one T. aestivum-L. mollis Robertsonian translocation chromosome, but the translocation chromosome in the two lines were different in morphology. The other one, WM-47 was a double heterozygous translocation with two different translocation chromosomes. By the C-banding, one of the three translocation lines was identified, the translocation chromosome consisted of 7DL of wheat and a chromosome arm of L. mollis. In addition, wheat chromosome deletions were observed in some plants. In another cross combination involved in common wheat and T. aestivum-Th. intermedium addition line, two alien terminal non-Robertsonian translocation lines (WI-21 and WI-68) were identified from 69 F2 plants by C-banding and GISH, and the percentage of translocation line was 2.90%. By C-banding analysis, the translocation chromosomes involved in wheat chromosomes 3A and 4A in the two lines, respectively. These results indicate that inducing wheat alien translocation line by gametocidal chromosome and irradiated pollens all are efficient methods.
Wang
Xian-Ping
XP
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xpwang@genetics.ac.cn
Chu
Jing-Hua
JH
Zhang
Xiang-Qi
XQ
D002871:Chromosome Banding; D017403:In Situ Hybridization; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*