The functional expression of human bone-derived cells grown on rapidly resorbable calcium phosphate ceramics.
The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation, since it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates on the expression of bone-related genes and proteins by human bone-derived cells (HBDC) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were alpha-TCP, and four materials which were created from beta-Rhenanite and its derivatives: R1-beta-Rhenanite (CaNaPO(4)); R1/M2 composed of CaNaPO(4) and MgNaPO(4); R1+SiO(2) composed of CaNaPO(4) and 9% SiO(2) (wt%); and R17-Ca(2)KNa(PO(4))(2). HBDC were grown on the substrata for 3, 5, 7, 14 and 21 days, counted and probed for various mRNAs and proteins (Type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrata supported continuous cellular growth for 21 days. At day 21, surfaces of R1+SiO(2) and R17 had the highest number of HBDC. At 14 and 21 days, cells on R1 and on R1+SiO(2) displayed significantly enhanced expression of all osteogenic proteins. Since all novel calcium phosphates supported cellular proliferation together with expression of bone-related proteins at least as much as TCP, these ceramics can be regarded as potential bone substitutes. R1 and R1+SiO(2) had the most effect on osteoblastic differentiation, thus suggesting that these materials may possess a higher potency to enhance osteogenesis than TCP.
Knabe
C
C
Department of Experimental Dentistry, Benjamin Franklin Hospital, Free University of Berlin, Assmannshauser Str. 4-6, 14197 Berlin, Federal Republic of Germany. christine.knabe@medizin.fu-berlin.de
Berger
G
G
Gildenhaar
R
R
Howlett
C R
CR
Markovic
B
B
Zreiqat
H
H
D018786:Bone Substitutes; D002130:Calcium Phosphates; D018929:Cell Culture Techniques; D002516:Ceramics; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D018786:Bone Substitutes; D002130:Calcium Phosphates; D012333:RNA, Messenger; C020243:calcium phosphate; C018392:tricalcium phosphate