Cobblestone area-forming cells in human cord blood are heterogeneous and differ from long-term culture-initiating cells.
The long-term culture-initiating cell (LTC-IC) assay is a physiological approach to the quantitation of primitive human hematopoietic cells. The readout using identification of cobblestone area-forming cells (CAFC) has gained popularity over the LTC-IC readout where cells are subcultured in a colony-forming cell assay. However, comparing the two assays, cord blood (CB) mononuclear cell (MNC) samples were found to contain a higher frequency of CAFC than LTC-IC (126 +/- 83 versus 40 +/- 31 per 10(5) cells, p = 0.0001). Overall, 60% of week-5 cobblestones produced by CB MNC were not functional LTC-IC and were classified as "false." Separation of CB MNC using immunomagnetic columns showed that false cobblestones were CD34(-)/lineage(+). Purified CD34(+) cells, as expected, gave very similar readouts in the two assays, with 4,084 and 3,468/10(5) cells being CAFC and LTC-IC, respectively. CD34(-)/lineage(-) cells did not form cobblestones or become CD34(+) on stroma or in cytokine culture. Human CB MNC contain a population of mature lineage(+) cells, possibly mature T or B cells, which, although producing cobblestone areas (CA), are not functional LTC-IC. The CAFC readout by this method, therefore, is unreliable for estimation of primitive hematopoietic cells by limiting dilution analysis in whole human CB or MNC and also may not detect CD34(-) CA stem cells.
University of Bristol, Division of Transplantation Sciences, Bristol, United Kingdom. P.A.Denning-Kendall@bristol.ac.uk
Patricia
P
Denning-Kendall
Sakon
S
Singha
Ben
B
Bradley
Jill
J
Hows
D018952:Antigens, CD34 / Q000502:physiology; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping
D018952:Antigens, CD34