A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.
The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.
GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA.
Shiaoching
S
Gong
Chen
C
Zheng
Martin L
ML
Doughty
Kasia
K
Losos
Nicholas
N
Didkovsky
Uta B
UB
Schambra
Norma J
NJ
Nowak
Alexandra
A
Joyner
Gabrielle
G
Leblanc
Mary E
ME
Hatten
Nathaniel
N
Heintz
D000818:Animals; D001369:Axons / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009488:Neurosciences / Q000379:methods; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000378:metabolism; D014157:Transcription Factors; D019076:Transgenes / Q000235:genetics*
D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; D060850:LIM-Homeodomain Proteins; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D010727:Phosphoric Diester Hydrolases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1