Characterization of human humoral responses to the major outer membrane protein and OMP2 of Chlamydophila pneumoniae.
Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral response include the major outer membrane protein (MOMP) and outer membrane protein 2 (OMP2). Using human anti-chlamydial sera we have defined the B cell epitopes recognized on MOMP and OMP2. Peptides from MOMP, unlike OMP2, were not strongly recognized. Two of these epitopes when linked to an inert carrier reacted strongly with high-titer anti-C. pneumoniae sera.
Department of Molecular Microbiology, University of Southampton, Tremona Rd, Southampton, SO16 6YD, UK. a.f.cunningham@bham.ac.uk
Adam F
AF
Cunningham
Michael E
ME
Ward
D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation / Q000276:immunology*; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016056:Immunodominant Epitopes
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D016056:Immunodominant Epitopes; C063931:Omp2 protein, bacteria; C101031:major outer membrane protein, Chlamydia pneumoniae