Duplex PCR-RFLP for simultaneous detection of factor V Leiden and prothrombin G20210A.
The authors have used a primer-engineered duplex polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) for the simultaneous detection of factor V Leiden and prothrombin G20210A mutations. The method involves the generation of HindIII RFLPs, and the restricted amplification products are analyzed by agarose gel electrophoresis in a single gel lane. This method is simple an inexpensive, and readily adaptable to the routine in a clinical molecular diagnostic laboratory other that our own.
Lucotte
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Centre de Neurogénétique Moléculaire, 44 rue Monge, 75005 Paris, France. lucotte@hotmail.com
Champenois
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D004587:Electrophoresis, Agar Gel; D005165:Factor V / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin