Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples.
This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody-antigen complexes were separated from free Fc-conjugated IgG antibody (Fc-IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200-2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.
Department of Biotechnology and Life Sciences, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
Tae-Kyu
TK
Lim
Hiroko
H
Ohta
Tadashi
T
Matsunaga
D004563:Electrochemistry / Q000295:instrumentation*; D005296:Ferrous Compounds / Q000737:chemistry; D006632:Histamine / Q000097:blood*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis; D000075163:Metallocenes; D008838:Microcomputers; D036103:Nanotechnology; D011058:Pollen / Q000276:immunology; D019904:Polymethyl Methacrylate
D005296:Ferrous Compounds; D007074:Immunoglobulin G; D000075163:Metallocenes; D006632:Histamine; D019904:Polymethyl Methacrylate; C004998:ferrocene