Direct retransformation of yeast with plasmid DNA isolated from single yeast colonies using rolling circle amplification.
We have efficiently amplified plasmid DNA from single yeast colonies using rolling circle amplification (RCA). The amplified DNA can be directly used for restriction digestion, DNA sequencing, or yeast transformation. The RCA-based high-fidelity amplification would be useful for plasmid manipulation in a variety of yeast-based systems, particularly for high-throughput analyses.
University of Florida, Gainesville, FL, USA.
Xiaodong
X
Ding
Anita K
AK
Snyder
Regina
R
Shaw
William G
WG
Farmerie
Wen-Yuan
WY
Song
D018920:Coculture Techniques / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D005818:Genetic Engineering / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification*; D015183:Restriction Mapping / Q000379:methods*; D014170:Transformation, Genetic
D004271:DNA, Fungal