Induction of myasthenia gravis in HLA transgenic mice by immunization with human acetylcholine receptors.
We utilized HLA transgenic mice to identify the dominant epitopes on the human (H)-AChR alpha subunit. The cytoplasmic H-AChR peptide alpha320-337 was the dominant T cell epitope for DQ8, DR3, and DQ8xDQ6 F1 mice. The H-AChR-immunized HLA-DQ8, DR3, DQ8xDR3 F1 and DQ8xDQ6 F1 mice developed clinical EAMG, whereas HLA-DQ6 mice were less susceptible.
Yang
Huan
H
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-1070, USA.
Goluszko
Elzbieta
E
David
Chella
C
Okita
David K
DK
Conti-Fine
Bianca
B
Chan
Teh-Sheng
TS
Poussin
Mathilde A
MA
Christadoss
Premkumar
P
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007114:Immunization / Q000379:methods; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology*; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D011950:Receptors, Cholinergic / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D010455:Peptides; D011950:Receptors, Cholinergic