Determination of aristolochic acids by capillary electrophoresis.
A method for the separation and quantification of aristolochic acids by capillary electrophoresis is described. Buffer solutions composed of sodium dihydrogen phosphate, sodium borate, and sodium dodecyl sulfate at pH 6.5-7 were found to be suitable for the separation of aristolochic acids, which can be well resolved in a few minutes. The separation and identification of six aristolochic acids contained in a commercial tincture called charrua, a traditional herbal medicine consisting of a hydroalcoholic extract of Aristolochia argentina, corroborate the usefulness of the method. The eventual toxic properties of the aristolochic acid containing charrua tincture are discussed.
Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Horacio A
HA
Priestap
Silvia L
SL
Iglesias
Martín F
MF
Desimone
Luis E
LE
Diaz
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D034341:Aristolochic Acids; D010936:Plant Extracts