Capillary electrophoresis as a simple and sensitive method to study polysaccharides of Sinorhizobium sp. NGR234.
We report the minimum amounts of polysaccharides (6.5 ng) that can be studied using capillary electrophoresis-laser-induced fluorescence (CE-LIF) and describe the separation of the various monosaccharides susceptible to occur in Sinorhizobium cell wall. On-gel hydrolysis of the polysaccharides is described. It allowed the easy and rapid determination of the monosaccharide composition of the excised lipopolysaccharides. The composition obtained fits with gas chromatography-mass spectrometry (GC-MS) results. This study underlines the simplicity of using CE-LIF, which remains an entirely "water medium" method, unlike GC-MS which is, in addition, less sensitive (at least 100-fold) than CE-LIF.
IMRCP, Université Paul Sabatier, Toulouse, France.
Nicolas
N
Fraysse
Christel
C
Verollet
François
F
Couderc
Véréna
V
Poinsot
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D008401:Gas Chromatography-Mass Spectrometry; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D020579:Sinorhizobium / Q000737:chemistry*
D009005:Monosaccharides; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial