Lipid synthesis and acyl-CoA synthetase in developing rice seeds.
Developing rice seeds rapidly accumulated storage lipids between 5 and 12 d after flowering. The contents of palmitic, oleic, and linoleic acids increased throughout seed development, while the alpha-linolenic acid content remained low. The activity of acyl-CoA synthetase varied coincidentally during the period of lipid accumulation, and rice seeds had a sufficient capacity to supply acyl-CoA substrates for TAG synthesis. Acyl-CoA synthetase showed a broad specificity for native FA of rice seeds except for stearic acid, and pi electrons of a delta9-delta11 double bond in the C16-C18 acyl chains were required for its maximal activity.
Biological Chemistry, Graduate School of Agricultural Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. ichihara@kpu.ac.jp
Ken'ichi
K
Ichihara
Noriaki
N
Kobayashi
Kazumi
K
Saito
D002462:Cell Membrane / Q000201:enzymology; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D012275:Oryza / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012639:Seeds / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D005227:Fatty Acids; D008055:Lipids; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase