AHL-deficient mutants of Burkholderia ambifaria BC-F have decreased antifungal activity.
Burkholderia ambifaria BC-F, a biocontrol strain reported previously to exhibit broad-spectrum antifungal activity, was highly active in formation of N-acyl homoserine lactones (AHLs). We constructed AHL-deficient derivatives of strain BC-F in which the genes specifying AHL synthase (bafI) and AHL-binding transcriptional activator (bafR) were inactivated by allelic exchange. The resulting AHL-deficient mutants had decreased antifungal activity.
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
Hongwei
H
Zhou
Fude
F
Yao
Daniel P
DP
Roberts
Thomas G
TG
Lessie
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D000898:Antibiosis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019117:Burkholderia / Q000235:genetics* / Q000502:physiology; D002876:Chromosomes, Bacterial; D005658:Fungi / Q000254:growth & development*; D023061:Gene Order; D008025:Ligases / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D001426:Bacterial Proteins; D015534:Trans-Activators; C088386:homoserine lactone; D008025:Ligases; C449816:N-acylhomoserine lactone synthase; D015107:4-Butyrolactone