Identification of cis-acting sequences required for selective packaging of bovine adenovirus type 3 DNA.
The assembly of adenovirus particles is a multistep process, in which viral genomic DNA is selected and subsequently inserted into preformed empty capsids. The selective encapsidation of the adenovirus genome is directed by cis-acting packaging motifs, termed A repeats due to their AT-rich character in DNA sequence. A repeats are usually located at the left end of the viral genome. In this report, the construction and analysis of bovine adenovirus type 3 (BAdV-3) mutants containing deletion mutations introduced into the AT-rich regions are described. The main cis-acting packaging domains of BAdV-3 were localized between nt 224 and 540 relative to the left end of the viral genome. They displayed a functional redundancy and followed a hierarchy of importance. In addition, the results demonstrated that not all of the AT-rich units functioned as cis-acting packaging motifs.
Xing
Li
L
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
Zhang
Linong
L
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
Kessel
Jill Van
JV
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
Tikoo
Suresh Kumar
SK
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
D001483:Base Sequence; D004279:DNA, Viral / Q000502:physiology*; D016679:Genome, Viral; D017921:Mastadenovirus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D012091:Repetitive Sequences, Nucleic Acid; D019065:Virus Assembly
D004279:DNA, Viral