Comparative studies on the internal defense system of schistosome-resistant and -susceptible amphibious snail Oncomelania nosophora 1. Comparative morphological and functional studies on hemocytes from both snails.
Two morphologically distinct blood cell types (hemocytes), Type I and Type II were found coexisting in hemolymph from two kinds of snails, Oncomelania nosophora strain, viz. from the Nirasaki strain (schistosome-resistant snail) and the Kisarazu strain (schistosome-susceptible snail). Ten min after inoculation of SRBC, the majority of Type I cells from Nirasaki strain flattened and spread over the surface of the glass plate by extending pseudopodia. In the Kisarazu strain, Type I cells adhered to the surface of substrate with spike-like filopodia, but did not form spreading lamellipodia. Type I cell from the Nirasaki strain phagocytosed SRBC but that from the Kisarazu strain did not. The starting time of recognition of foreign materials was slightly different in the Type I hemocytes from the two strains. Type II cells from both strains were round and lymphocyte-like. Ten or sixty min after incubation, Type II cells from neither strain adhered to the surface of substrate or SRBC, and did not phagocytose SRBC. Type II cells from the Nirasaki strain were quite similar to those from the Kisarazu strain. We concluded that Type I cells from the schistosome-resistant snail, Nirasaki strain, possessed higher phagocytic activity than those from the susceptible snail, Kisarazu strain, despite the morphological similarities of the hemocytes from both strains.
Department of Biology, Tokyo Medical University, Shinjuku, Tokyo, Japan. yuri-s@tokyo-med.ac.jp
Yuri
Y
Sasaki
Emiko
E
Furuta
Masashi
M
Kirinoki
Naomi
N
Seo
Hajime
H
Matsuda
D000818:Animals; D004198:Disease Susceptibility / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006434:Hemocytes / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure; D007564:Japan; D008854:Microscopy, Electron; D010587:Phagocytosis / Q000502:physiology*; D012908:Snails / Q000276:immunology* / Q000469:parasitology