A method for the automatic characterization of bone architecture in 3D mice microtomographic images.
We developed an automatic method to characterize mice bone architecture from three-dimensional (3D) microtomographic images. The distal metaphyses of the femur of mice were imaged using 3D synchrotron radiation microtomography at the European Synchrotron Radiation Facility (ID19) with a voxel size of 6.65 mum. Within each reconstructed volume, a region of interest was defined and trabecular and cortical bones were automatically separated. Then, 3D morphologic and topologic model-independent parameters quantifying the 3D bone architecture were computed in both regions. The technique was applied to study the response of the C57BL/6J@Ico strain of mice submitted to a model of bone loss by hind limb unloading produced by tail-suspension.
ESRF (European Synchrotron Radiation Facility), BP 220, 38043 Grenoble, France. estela@optica.fao.ub.es
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E
Martín-Badosa
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A
Elmoutaouakkil
S
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Nuzzo
D
D
Amblard
L
L
Vico
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Peyrin
D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000473:pathology; D005269:Femur / Q000000981:diagnostic imaging; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008852:Microradiography; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000473:pathology; D014057:Tomography, X-Ray Computed