Chemokine receptor CCR5 is not required for development of experimental autoimmune gastritis.
Experimental autoimmune gastritis (EAG) is a model of human autoimmune gastritis, the underlying cause of pernicious anaemia. It is characterised by gastric mononuclear cell infiltrates, destruction of parietal and zymogenic cells, and autoantibodies to parietal cell-associated H(+)/K(+) ATPase. Here, we have investigated the role of CCR5 in the development of EAG. We found that the development of EAG was not prevented in CCR5-deficient mice. Using reverse-transcriptase analysis of stomachs from normal and gastritic mice we found no difference in expression of CCR5 and its chemokine ligands MIP-1alpha, MIP-1beta, and RANTES. We also found that the CCR5 antagonist met-RANTES failed to prevent the development of EAG induced by neonatal thymectomy. These observations suggest that the CC chemokine receptor CCR5 is not essential for development of EAG.
Field
Judith
J
Monash University, Department of Pathology and Immunology, Central and Eastern Clinical School, AMREP Commercial Road, Prahran, 3181 Victoria, Australia.
Marshall
Aiden C J
AC
Hertzog
Paul J
PJ
Wells
Timothy N
TN
Alderuccio
Frank
F
Toh
Ban Hock
BH
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D065100:CCR5 Receptor Antagonists; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D003433:Crosses, Genetic; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005756:Gastritis / Q000276:immunology* / Q000473:pathology; D017506:H(+)-K(+)-Exchanging ATPase / Q000276:immunology / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010295:Parietal Cells, Gastric / Q000276:immunology; D019713:Receptors, CCR5 / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000276:immunology / Q000473:pathology
D065100:CCR5 Receptor Antagonists; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019402:Macrophage Inflammatory Proteins; C108335:RANTES, Met-; D019713:Receptors, CCR5; D004247:DNA; D017506:H(+)-K(+)-Exchanging ATPase