Expression of a novel lacrimal gland gene lacritin in human breast tissues.
OBJECTIVE
The purpose of this study was to examine breast tumors and normal breast tissues for the expression of the glycoprotein lacritin. The mRNA and protein expression of lacritin has been reported to be restricted to the lacrimal gland.


METHODS
We investigated 37 human primary invasive breast tumors, seven breast cancer cell lines and 16 normal breast tissues by quantitative real-time PCR for lacritin expression.


RESULTS
We detected lacritin transcripts in 51% (19/37) of the primary invasive breast tumors, in 71% (5/7) of the breast cancer cell lines, and also in 56% (9/16) of the normal breast tissues. No lacritin mRNA was detectable in peripheral blood of healthy individuals.


CONCLUSIONS
Here we show by quantitative real-time PCR that lacritin is expressed in human breast tumors, breast cancer cell lines, and normal breast. The previously reported restricted expression pattern of lacritin is therefore incorrect. Lacritin transcripts were not detected in peripheral blood which makes lacritin a potential candidate as a breast cancer marker gene.
Division of Experimental Therapy, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
B
B
Weigelt
A J
AJ
Bosma
L J
LJ
van 't Veer
D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
D014408:Biomarkers, Tumor; D006023:Glycoproteins; C431764:LACRT protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm