Dose-response relationship in lethal and behavioural effects of different insecticides on the parasitic wasp Aphidius ervi.
Neurotoxic insecticides are widely used for crop protection and behavioural perturbations can be expected in surviving beneficial insects, including parasitoids of pest insects. The present study aims to investigate the relationship between the dose of insecticide parasitoids have been exposed to, and the subsequent ability of these parasitoids to respond to host-related cues. A four-armed olfactometer, a design widely used to observe orientation responses in various insects and parasitoids in particular, was chosen to investigate the dose-response relationship. The species studied was Aphidius ervi, a relatively generalist parasitoid of aphids, and commercialised for biological control and integrated pest management. Active ingredients with similar and different modes of action on the nervous system were compared: a pyrethroid (lambda-cyhalothrin), an organophosphate (chlorpyrifos), a carbamate (pirimicarb) and a carbamyltriazole (triazamate). Adult females were exposed to dry residues on glass for 24 h. LD50 were calculated and predicted a high risk of mortality at the field application rate. The effect of five increasing residual doses of each active ingredient was tested on responses to plant-host odour in the olfactometer, from sublethal doses to LD50, and up to LD70 for some products. It appeared that none of the doses of lambda-cyhalothrin, chlorpyriphos and pirimicarb had any effect on A. ervi responses to the odour from the aphid-infested plant (Myzus persicae on oilseed rape). But for triazamate, a significant dose-behavioural response was quantified and attraction to the odour was no longer significant in females surviving the LD50. The possible explanations for the presence or absence of effect, depending on the insecticide are discussed.
Laboratoire de Neurobiologie Comparée des Invertébrés, INRA, BP 23, 91440 Bures-sur-Yvette, France. desneux@jouy.inra.fr
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Desneux
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Rafalimanana
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Kaiser
D000818:Animals; D001042:Aphids / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D009949:Orientation / Q000187:drug effects*; D012903:Smell / Q000187:drug effects*; D023382:Toxicity Tests, Acute; D014863:Wasps / Q000187:drug effects / Q000502:physiology*
D007306:Insecticides