[Study on autoimmune thrombocytopenic purpura related antibody].
OBJECTIVE
To investigate a specific and sensitive assay for the diagnosis of autoimmune thrombocytopenic purpura (AITP).


METHODS
Glycoprotein specific autoantibodies in platelet eluate and plasma were detected by a modified monoclonal antibody immobilization of platelet antigens assay (MAIPA).


RESULTS
The overall positive rate of specific autoantibodies against platelet GPIIb/IIIa and GPIb/IX in plasma was 38.89% and in eluated platelet membrane was 68.52%. The difference between them was significant (corrected chi(2) = 19.39, P < 0.005). The proportion of positive MAIPA results between primary AITP and secondary AITP was not significantly different. There was a significant inverse correlation between antibody level and platelet count.


CONCLUSIONS
Detection of eluated GP-specific autoantibodies by MAIPA is highly specific and much more sensitive as compared with the measurement of their plasma counterparts in the diagnosing and therapeutic monitoring of AITP.
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Lu
L
Lu
Ming
M
Hou
Yan
Y
Shi
Ping
P
Qin
Jun
J
Peng
Yuan-yuan
YY
Zhu
Mao-hong
MH
Zhang
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*
D001323:Autoantibodies; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex