Synthesis of fluorinated analogs of myristic acid as potential inhibitors of Egyptian armyworm (Spodoptera littoralis) delta11 desaturase.
To study the activity of the different desaturases present in the pheromone biosynthetic pathway of the Egyptian armyworm, Spodoptera littoralis, we prepared a series of mono- and gem-difluorinated analogs of myristic acid with halogen substitution at the C8-C11 positions of the aliphatic chain via specifically positioned dithiane precursors. Thus, transformation of dithianes by treatment with N-bromosuccinimide in the presence of H2O followed by reduction with LiAlH4 afforded the appropriate alcohols, which reacted with diethylaminosulfur trifluoride to give rise to the corresponding monofluoroderivative intermediates. Alternatively, the introduction of the gem-difluoro functionality was carried out by reaction of the appropriate dithiane intermediate with 1,3-dibromo-5,5-dimethylhydantoin in the presence of HF/pyridine. The activity of these fluorinated FA as substrates and inhibitors of the desaturases involved in the biosynthesis of the sex pheromonal blend of S. littoralis has been studied. In this case, 11-fluorotetradecanoic acid elicited a moderate inhibitory activity of delta11 desaturase.
Abad
José-Luis
JL
Departamento de Química Orgánica Biológica, Instituto de Investigaciones Químicas y Ambientales de Barcelona, Consejo Superior de Investigaciones Científicas, 08034, Barcelona, Spain.
Villorbina
Gemma
G
Fabriàs
Gemma
G
Camps
Francisco
F
D000818:Animals; D004534:Egypt; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005461:Fluorine / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D019814:Myristic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D018411:Spodoptera / Q000201:enzymology*
D004791:Enzyme Inhibitors; D019814:Myristic Acid; D005461:Fluorine; D044943:Fatty Acid Desaturases