[Use of 1- and 2-thionaphthylacetates as cholinesterase substrates].
1- and 2-thionaphthylacetates were tested as cholinesterase substrates. It was shown that the butyrilcholinesterase from horse serum can hydrolize these compounds. The hydrolysis velocity of 1-thionaphthylacetate was comparable with hydrolysis velocity of acetylthiocholine (the well known cholinesterase substrate), but 2-thionaphthylacetate was hydrolysed more slowly. The values of the kinetic parameters V and K(m) for butyrylcholinesterase hydrolysis of 1- and 2-thionaphthylacetates were determined. It was offered to use 1-thionaphthylacetates as the substrate for cholinesterases.
Zhukovskiĭ
Iu G
IuG
Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, St. Petersburg, Russian Federation. zhuk@zhuk.mail.iephb.ru
Kuznetsova
L P
LP
Sochilina
E E
EE
Veksler
K V
KV
D000122:Acetylthiocholine / Q000737:chemistry; D000818:Animals; D002091:Butyrylcholinesterase; D002800:Cholinesterase Inhibitors / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009280:Naphthaleneacetic Acids / Q000737:chemistry; D009281:Naphthalenes / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature
C482606:1-thionaphthylacetate; D002800:Cholinesterase Inhibitors; D009280:Naphthaleneacetic Acids; D009281:Naphthalenes; C034182:1-naphthaleneacetic acid; D000122:Acetylthiocholine; C002688:2-naphthylthiolacetate; D002091:Butyrylcholinesterase