Detection of cryptic MLL insertions using a commercial dual-color fluorescence in situ hybridization probe.
Involvement of the MLL gene located at chromosome region 11q23 is a frequent occurrence in both acute myelocytic leukemia and acute lymphoblastic leukemia. More than 30 loci have now been associated with MLL, usually by reciprocal translocation. Deletions, insertions, and more complex rearrangements of MLL are rarely seen. We present three cases of AML M5 showing no cytogenetic evidence of 11q23 rearrangement, in which a commercial MLL dual-color fluorescence in situ hybridization probe revealed a nonstandard abnormal signal pattern, suggesting cryptic insertion of the MLL gene into its partner gene site.
North Trent Cytogenetics Department, Sheffield Children's NHS Trust, Western Bank, S10 2TH, Sheffield, UK. mike.dyson@sch.nhs.uk
Michael J
MJ
Dyson
Polly J
PJ
Talley
John T
JT
Reilly
David
D
Stevenson
Emma
E
Parsons
Jane
J
Tighe
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000473:pathology; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D051788:Myeloid-Lymphoid Leukemia Protein; D011519:Proto-Oncogenes; D016615:Telomere / Q000235:genetics; D014157:Transcription Factors
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C497422:KMT2A protein, human; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase