Presence of beta-arrestin in cellular inclusions in metamphetamine-treated PC12 cells.
Cellular inclusions containing ubiquitin and alpha-synuclein were observed in PC12 cells treated with metamphetamine (MA). To study the possible involvement of beta-arrestin in inclusion formation, we treated PC12 cells with MA for different times and analyzed the ubiquitin proteosome pathway (UPP). We found that beta-arrestin is ubiquitinated in the MA-treated PC12 cell line. The involvement of beta-arrestin in UPP was further supported by electron microscopy and by confocal microscopy, which documented the presence of beta-arrestin in these Lewy body-like inclusions. Our experiments reveal an interesting and previously unappreciated connection between beta-arrestin and ubiquitination and suggest that beta-arrestin could be involved in the development of the inclusion bodies.
I.N.M. Neuromed, località Camerelle, Pozzilli (IS), Italy.
A
A
De Blasi
L
L
Capobianco
L
L
Iacovelli
P
P
Lenzi
M
M
Ferrucci
G
G
Lazzeri
F
F
Fornai
A
A
Picascia
D000818:Animals; D019390:Arrestins / Q000032:analysis*; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004195:Disease Models, Animal; D002479:Inclusion Bodies / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008694:Methamphetamine / Q000494:pharmacology*; D019636:Neurodegenerative Diseases; D016716:PC12 Cells; D051381:Rats; D015398:Signal Transduction; D013997:Time Factors; D025801:Ubiquitin / Q000378:metabolism; D000071557:beta-Arrestins
D019390:Arrestins; D000697:Central Nervous System Stimulants; D025801:Ubiquitin; D000071557:beta-Arrestins; D008694:Methamphetamine