The effect of glucocorticoids on the myosin heavy chain isoforms' turnover in skeletal muscle.
The purpose of this study was to find the effect of dexamethasone on the myosin heavy chain (MyHC) isoforms' composition in different skeletal muscles and glycolytic (G) fibres in relation with their synthesis rate and degradation of MyHC isoforms by alkaline proteinases. Eighteen-week-old male rats of the Wistar strain were treated with dexamethasone (100 microg/100 g bwt) during 10 days. The forelimb strength decreased from 9.52 to 6.19 N (P<0.001) and hindlimb strength from 15.54 to 8.55 N (P<0.001). Daily motor activity decreased (total activity from 933 to 559 and ambulatory activity from 482 to 226 movements/h, P<0.001). The degradation rate of muscle contractile proteins increased from 2.0 to 5.9% per day (P<0.001), as well as the myosin heavy chain IIB isoform degradation with alkaline proteinase in fast-twitch (F-T) muscles (12 +/- 0.9%; P<0.05) and glycolytic muscle fibres (15 +/- 1.1%; P<0.001). The synthesis rate of MyHC type II isoforms decreased in Pla muscles (P<0.05) and MyHC IIA (P<0.05) and IIB in EDL muscle and G fibres (P<0.001). The relative content of MyHC IIB isoform decreased in F-T muscles (P<0.001) and in G fibres (P<0.01), and the relative content of IIA and IID isoforms increased simultaneously. Dexamethasone decreased the MyHC IIB isoform synthesis rate and increased the sensibility of MyHC IIB isoform to alkaline proteinase, which in its turn led to the decrease of MyHC IIB isoform relative content in F-T muscles with low oxidative potential and G muscle fibres.
Seene
Teet
T
Department of Functional Morphology, University of Tartu, Ylikooli Str 18, Tartu 50090, Estonia. teet.seene@ut.ee
Kaasik
Priit
P
Pehme
Ando
A
Alev
Karin
K
Riso
Eva-Maria
EM
D000818:Animals; D003285:Contractile Proteins / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism; D005552:Forelimb; D005938:Glucocorticoids / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009068:Movement / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018995:Myosin Heavy Chains / Q000187:drug effects / Q000378:metabolism*; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D003285:Contractile Proteins; D005938:Glucocorticoids; D020033:Protein Isoforms; D003907:Dexamethasone; D010450:Endopeptidases; D018995:Myosin Heavy Chains