A cDNA encoding vacuolar type beta-D-fructofuranosidase (Os beta fruct3) of rice and its expression in Pichia pastoris.
A vacuolar type beta-D-fructofuranosidase (Os beta fruct3) was cloned from etiolated rice seedlings cDNA library. It encodes an open reading frame of 688 residues. The deduced amino acid sequence had 58% identity to the vacuolar type beta-D-fructofuranosidase of maize (Ivr1). Os beta fruct3 exists as a single copy per genome. Northern analyses showed that Os beta fruct3 undergoes organ-specific expression and is involved in the adjustment of plant responses to environmental signals and metabolizable sugars. Os beta fruct3 was also heterologously expressed in Pichia pastoris. The recombinant proteins were confirmed to be a vacuolar type beta-D-fructofuranosidase.
Institute of Agricultural Chemistry, National Taiwan University No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan. f85623601@ms86.ntu.edu.tw
Ru-Huei
RH
Fu
Ai-Yu
AY
Wang
Yu-Chi
YC
Wang
Hsien-Yi
HY
Sung
D000595:Amino Acid Sequence; D003001:Cloning, Molecular / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D015723:Gene Library; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D010843:Pichia / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D043324:beta-Fructofuranosidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
D011994:Recombinant Proteins; D043324:beta-Fructofuranosidase