Cytochrome P450 2J2 polymorphism in healthy Caucasians and those with diabetes mellitus.
OBJECTIVE
Cytochrome P450 (CYP) 2J2 plays an important role in the biosynthesis of the biologically active cis-epoxyeicosatrienoic acids. An allelic variant named CYP2J2*6, which encodes an enzyme that is almost inactive in the metabolism of arachidonic acid, has recently been described. We investigated the frequency of the CYP2J2*6 variant in a Caucasian population and the relationship between this polymorphism and the development of micro- and macrovascular complications and hypertension in patients with type 1 or type 2 diabetes mellitus.


METHODS
Genomic DNA was extracted from peripheral blood cells and the fragment containing the A/T single nucleotide polymorphism at position 25 661 in exon 8 of the CYP2J2 gene was amplified. The 532 bp amplified product was subsequently digested with Tsp509I and analyzed on 12% polyacrylamide gel electrophoresis.


RESULTS
In the whole population, the frequency of the CYP2J2*6 allele was 0.0064 and the frequency of the CYP2J2*1 allele was 0.9936. Genotype distribution did not show significant differences between controls and patients with type 1 or type 2 diabetes. No homozygotes for CYP2J2*6 allele were found. No association was found between this allele and complications or hypertension in either type of diabetes.


CONCLUSIONS
The CYP2J2*6 allele is rare in the Caucasian population, and no association is inferred between this allelic variant and diabetic complications.
Pucci
Laura
L
Department of Endocrinology and Metabolism, Unit of Diabetes and Metabolism, Faculty of Medicine, University of Pisa, Pisa, Italy.
Lucchesi
Daniela
D
Chirulli
Vera
V
Penno
Giuseppe
G
Johansson
Inger
I
Gervasi
Pier
P
Del Prato
Stefano
S
Longo
Vincenzo
V
D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D006580:Genetic Carrier Screening; D014644:Genetic Variation; D006801:Humans; D010105:Oxygenases / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D044465:White People / Q000235:genetics*
C000717350:CYP2J2 protein, human; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2