1,5-Anhydroglucitol stimulates insulin release in insulinoma cell lines.
Concentrations of 1,5-anhydroglucitol (1,5-AG), which is a major circulating polyol, decrease in patients with diabetes mellitus. In both insulinoma-derived RINr and MIN6 cells, 1,5-AG stimulated insulin release within the range of 0.03-0.61 mM in a dose-dependent manner. Insulin release was maximally stimulated by 1,5-AG to levels that reached 25% and 100% greater than that of control (1,5-AG-free group) in RINr and MIN6 cells, respectively. A physiological concentration of 1,5-AG stimulated insulin release after a 5-min incubation and this action was maintained for 60 min. In addition, at approximately 1/200 the concentration of glucose, 1,5-AG had additive action with 20 mM glucose. The action of 1,5-AG on insulin secretion with other types of saccharides and polyol was similarly additive. Mannnoheptulose and diazoxide suppressed the stimulative action of 1,5-AG on insulin release. The secretagogue action of 1,5-AG seemed to be independent on an increase in the intracellular content of cAMP and ATP. These results suggest that 1,5-AG can stimulate insulin secretion through a mechanism that completely differs from that of glucose.
Department of Internal Medicine, University of Teikyo, Kaga, Tokyo 173-0003, Itabashi, Japan. yama@med.teikyo-u.ac.jp
Toshikazu
T
Yamanouchi
Tae
T
Inoue
Kaoru
K
Ichiyanagi
Tadashi
T
Sakai
Nobuyuki
N
Ogata
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D003847:Deoxyglucose / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D014993:Xylitol / Q000494:pharmacology
D002241:Carbohydrates; D007328:Insulin; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose; D014993:Xylitol