[Treatment of surface burns with proteolytic enzymes: mathematic description of lysis kinetics].
The lysis of necrotic tissue by a proteolytic enzyme applied to the surface of a burn wound was studied. A mathematical model was proposed, which describes changes in the thickness of necrotic tissue as a function of the proteolytic activity of the enzyme. The model takes into account the inward-directed diffusion of the enzyme, the counterflow of interstitial fluid (exudates) containing specific inhibitors, and the extracellular matrix proteolysis. It was shown in terms of the quasi-stationary approach that the thickness of the necrotic tissue layer decreases exponentially with time; i.e., the lysis slows down as the thickness of the necrotic tissue layer decreases. The dependence of the characteristic time of this decrease on enzyme concentration was obtained. It was shown that, at high enzyme concentrations (more than 5 mg/ml), the entire time of lysis (after the establishment of quasi-stationary equilibrium) is inversely proportional to the concentration of the enzyme.
Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119899 Russia. domogatskaya@fromru.com
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Domogatskaia
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Domogatskiĭ
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Ruuge
D002056:Burns / Q000201:enzymology* / Q000473:pathology; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D009336:Necrosis