Positional cloning of the major quantitative trait locus underlying lung tumor susceptibility in mice.
Pulmonary adenoma susceptibility 1 (Pas1), located on chromosome 6, is the major locus affecting inherited predisposition to lung tumor development in mice. We have fine mapped the Pas1 locus to a region of approximately 0.5 megabases by using congenic strains of mice, constructed by placing the Pas1 region of chromosome 6 from A/J mice onto the genetic background of C57BL/6J mice. Systematic characterization of Pas1 candidates establishes the Las1 (lung adenoma susceptibility 1) and Kras2 (Kirsten rat sarcoma oncogene 2) genes as primary candidates for the Pas1 locus. Clearly, Kras2 affects lung tumor progression only, and Las1 is likely to affect lung tumor multiplicity.
Zhang
Zhongqiu
Z
Department of Surgery and The Alvin J. Siteman Cancer Center, Washington University School of Medicine, Campus Box 8109, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Futamura
Manabu
M
Vikis
Haris G
HG
Wang
Min
M
Li
Jie
J
Wang
Yian
Y
Guan
Kun-Liang
KL
You
Ming
M
D000236:Adenoma / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002938:Ciona intestinalis / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D040641:Quantitative Trait Loci; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018631:ras Proteins
D017931:DNA Primers; D005819:Genetic Markers; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins