Reduced accumulation of specific microRNAs in colorectal neoplasia.
Short non-coding RNAs are known to regulate cellular processes including development, heterochromatin formation, and genomic stability in eukaryotes. Given the impact of these processes on cellular identity, a study was undertaken to investigate possible changes in microRNA (miRNA) levels during tumorigenesis. A total of 28 different miRNA sequences was identified in a colonic adenocarcinoma and normal mucosa, including 3 novel sequences and a further 7 that had previously been cloned only from mice. Human homologues of murine miRNA sequences, miR-143 and miR-145, consistently display reduced steady-state levels of the mature miRNA at the adenomatous and cancer stages of colorectal neoplasia.
Department of Gastroenterology and Hepatology, Flinders Centre for Digestive Health, Flinders Medical Centre, South Australia, Australia. michael.michael@flinders.edu.au
Michael Z
MZ
Michael
Susan M
SM
O' Connor
Nicholas G
NG
van Holst Pellekaan
Graeme P
GP
Young
Robert J
RJ
James
D000230:Adenocarcinoma / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D035683:MicroRNAs / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data
D035683:MicroRNAs