[Detection and differentiation of the bovine parainfluenza-3 virus strains studied by amplification and sequencing of the HN gene].
A possibility of using the amplification of gene HN fragment in combination with nucleotide cDNA sequencing for the purpose of identification and strain differentiation of bovine parainfluenza-3 virus was demonstrated. A comparative analysis of the primary structure in the studied HN gene fragment revealed 2 genetic groups among the investigated virus' strains and isolates. Group 1 is made up of Northern American viral strains and of Russian isolates, whose primary structure has a high level of homology to the primary SF-4/32 strain structure; group 2 comprises the virus' Russian isolates with a high level of homology to the mentioned strains to Japanese strains' sequences. The biggest differences between the studied strains and the viral isolates amounted to around 8%, when the nucleotide sequences were compared, and to around 4%, when the corresponding amino-acid sequences were compared.
A E
AE
Vecherov
P K
PK
Aianot
A M
AM
Timina
V V
VV
Lisitsin
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D014644:Genetic Variation; D015707:HN Protein / Q000235:genetics*; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D009297:Nasal Mucosa / Q000378:metabolism / Q000821:virology; D029101:Parainfluenza Virus 3, Bovine / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D010253:Respirovirus Infections / Q000662:veterinary* / Q000821:virology; D012426:Russia / Q000453:epidemiology; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017385:Sequence Homology; D013154:Spleen / Q000821:virology
D017931:DNA Primers; D018076:DNA, Complementary; D015707:HN Protein