Synthesis of novel fluorescent probes for the molecular chaperone Hsp90.
Heat shock protein 90 (Hsp90) is a molecular chaperone necessary for maintaining oncogenic transformation. There is substantial interest in developing novel agents that bind to the N-terminal of the chaperone. Here we report the synthesis and characterization of two fluorescent Hsp90 inhibitors and probe their use in an Hsp90 fluorescent polarization assay.
Llauger-Bufi
Laura
L
Program in Cell Biology and Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Felts
Sara J
SJ
Huezo
Henri
H
Rosen
Neal
N
Chiosis
Gabriela
G
D001667:Binding, Competitive; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000187:drug effects; D007700:Kinetics; D015394:Molecular Structure; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins