Anti malondialdehyde-adduct immunological response as a possible marker of successful aging.
Contrasting results have been obtained by various researchers about oxidative markers of aging. In this study, a healthy over-90-year-old population was examined for various plasma oxidative biomarkers and compared with a healthy population of blood donors (age range 23-66). Plasma malondialdehyde (MDA), evaluated by means of the thiobarbituric acid test, was significantly higher in the over-90-year-old population, confirming the presence of increased lipoperoxidation in old age. The antibody titre against MDA-protein adducts, considered a marker of lipoperoxidative protein damage in vivo, was evaluated in an ELISA test, completely home made and calibrated versus a concentrated pool of human plasma; this antibody titre was significantly higher in the over-90-year-old population. Plasma vitamin E, evaluated in RP-HPLC, was not significantly different between the two groups. Plasma protein-bound carbonyls, a marker of oxidative protein damage, were measured with the 2,4-dinitrophenylhydrazine assay; their level in the over-90-year-old population was lower than in the blood donors. The higher antibody titre against MDA-adducts may result in protection against accumulation of oxidatively damaged proteins by enhancing their removal, and, together with the preserved plasma vitamin E level, it may endow over-90-year-olds with an especially efficient antioxidant profile. The low level of protein carbonyl might reflect the more efficient removal of damaged proteins.
Department of Experimental Medicine, Section of General Pathology, University of Genova, Genova, Italy. ntravers@medicina.unige.it
Nicola
N
Traverso
Stefania
S
Patriarca
Emanuela
E
Balbis
Anna Lisa
AL
Furfaro
Damiano
D
Cottalasso
Maria Adelaide
MA
Pronzato
Paolo
P
Carlier
Federica
F
Botta
Umberto Maria
UM
Marinari
Luigi
L
Fontana
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D012372:ROC Curve; D014810:Vitamin E / Q000097:blood
D001323:Autoantibodies; D015415:Biomarkers; D001798:Blood Proteins; D014810:Vitamin E; D008315:Malondialdehyde