Dengue virus type 2 infects human endothelial cells through binding of the viral envelope glycoprotein to cell surface polypeptides.
The endothelial cell line ECV304, derived from human umbilical cord and identified to be susceptible to dengue virus type 2 (DEN-2) infection, was used to study the molecular mechanism of DEN-2 binding to endothelial cells. DEN-2 was found by virus overlay protein-binding assays (VOPBAs) to bind to three ECV304 cell membrane proteins with molecular masses of 29, 34 and 43 kDa. Only a single protein of 29 kDa was observed when VOPBAs were carried out using preparations of trypsin-treated ECV304 cells. Pre-incubation of live ECV304 cells in culture or cell membrane proteins in modified VOPBAs with the recombinant DEN-2 envelope glycoprotein (rEgp) inhibited DEN-2 infection and blocked virus binding to the three proteins identified. These results indicate that DEN-2 rEgp could bind to three proteins on the surface of ECV304 cells. This virus-cell interaction may be associated with the receptor complex specific for DEN-2 infection of endothelial cells.
Wei
Hui-Yong
HY
Medical Laboratory Center, Guangdong Provincial People's Hospital, 106 Zhongshan Road II, Guangzhou 510080, People's Republic of China.|Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
Jiang
Li-Fang
LF
Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
Fang
Dan-Yun
DY
Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
Guo
Hui-Yu
HY
Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology / Q000821:virology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*
D008565:Membrane Proteins; D011991:Receptors, Virus; D014759:Viral Envelope Proteins