Intrathecal synthesis of monocyte chemoattractant protein-1 (MCP-1) in amyotrophic lateral sclerosis: further evidence for microglial activation in neurodegeneration.
Autopsy studies and animal experiments suggest that microglial inflammation contributes to the pathogenesis of amyotrophic lateral sclerosis (ALS). Monocyte-chemoattractant protein (MCP-1) might play an important role in microglial recruitment. We studied MCP-1 levels in sera and cerebrospinal fluid of 29 ALS patients and compared the results with 11 control patients with tension headache. The MCP-1 level was determined using enzyme-linked immunosorbent assays (ELISA). A significant increase in cerebrospinal fluid MCP-1 level but not serum level was seen in the patients with ALS compared to the control subjects. These results suggest that cerebrospinal fluid MCP-1 activity may be a sensitive marker for neuroinflammation in ALS useful for monitoring treatment trials in ALS.
Klinik für Neurologie, Christian-Albrechts-Universität Kiel, Niemannsweg 147, 24105 Kiel, Germany. h.wilms@neurologie.uni-kiel.de
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H
Wilms
J
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Sievers
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Dengler
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J
Bufler
G
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Deuschl
R
R
Lucius
D017668:Age of Onset; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000473:pathology*; D000704:Analysis of Variance; D002465:Cell Movement / Q000276:immunology; D002556:Cerebrospinal Fluid Proteins / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D017628:Microglia / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D015854:Up-Regulation / Q000276:immunology
D002556:Cerebrospinal Fluid Proteins; D018932:Chemokine CCL2