Production of oligosaccharides by autohydrolysis of brewery's spent grain.
Brewery's spent grain was treated with water in a process oriented towards the production of xylo-oligosaccharides (XOS). A wide range of temperatures and reaction times were tested and the effects of these operational variables on hemicellulose solubilization and reaction products were investigated. The maximal XOS yield (61% of the feedstock xylan) was obtained at 190 degrees C after 5 min of reaction. Several oligosaccharide mixtures with different molecular weight distributions were obtained depending on temperature and reaction time. Longer reaction times led to decreased oligosaccharide production and enhanced concentrations of monosaccharides, sugar decomposition products and acetic acid. With reaction times leading to the maximal yields of XOS, little decomposition into organic acids and aldehydes was found at all the temperatures assayed. From the composition of processed solids, it was calculated that 63-77% of the initial xylan was selectively solubilized in autohydrolysis treatments.
Departamento de Biotecnologia, INETI, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
F
F
Carvalheiro
M P
MP
Esteves
J C
JC
Parajó
H
H
Pereira
F M
FM
Gírio
D001467:Hordeum / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D007220:Industrial Waste; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors
D007220:Industrial Waste; D009844:Oligosaccharides