Quantitative measurement of biotin-binding immunoglobulin G in human sera using F(ab')2 anti-human IgG-coated multi-well plates.
Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avidin-biotinylated peroxidase complex with method A, B, or C, respectively. Commercially available B-IgG was detected by all these three methods. However, method A and B could not detect B-IgG in the human sera used in this study, but we quantitatively measured the B-IgG level using method C. The result is probably due to the fact that the sensitivity of method C was higher than that of methods A or B. Properties of B-IgG detected by method C are discussed in the text.
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture College of Nursing, Habikino, Osaka, Japan. mmakoto@osaka-hsu.ac.jp
Makoto
M
Muratsugu
Eri
E
Muramoto
Toru
T
Fukui
D001666:Binding Sites, Antibody; D001710:Biotin / Q000097:blood*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011485:Protein Binding / Q000276:immunology; D011971:Receptors, Immunologic / Q000097:blood*
C052643:F(ab')2 receptor; D007074:Immunoglobulin G; D011971:Receptors, Immunologic; D001710:Biotin