Effects of hepatectomy and arginine enriched diet on colonic mucosa originated inflammatory cytokines.
OBJECTIVE
To assess the effects of arginine-enriched diet and partial hepatectomy in rats on gut-originated inflammatory cytokines.


METHODS
Of 24 rats, Group 1 and 2 animals were fasted 24 hours before surgery, Group 3 and 4 animals received regular plus arginine-enriched diet(AED). Group 2 and 4 animals had undergone 30% hepatic resection. Leukotriene B4 (LT B4) levels were detected in colonic mucosa and mucosal perfusates immediately after resection. Mean leukocyte counts (MLC) were detected also in the mucosa.


RESULTS
In the basis of fasting situation regardless hepatectomy, all MLC were lower in Group 3 and 4 but LTB4 levels both in mucosa and perfusate were higher. On hepatectomy based comparison there was not any statistically significant difference between groups but mucosal perfusate LTB4 levels. But when hepatectomy added on fasted animals MLC levels were lower than fed by AED + sham operation. LTB4 levels were insignificant in both perfusate and mucosa. When hepatectomy added on AED animals (Group 4), MLC decreased and mucosal LTB4 increased when they compared with fast without hepatectomy Group 1.


CONCLUSIONS
AED prior to extra-intestinal operations may trigger inflammatory cascade and complications via leucocyte degradation and LTB4.
Aydin
Cengiz
C
Dokuz Eylul University Hospital, Dept. of Surgery, Izmir, Turkey.
Coker
Ahmet
A
Atilla
Koray
K
Ozer
Erdener
E
Coker
Isil
I
Huseyinov
Afig
A
D000818:Animals; D001120:Arginine / Q000008:administration & dosage*; D003106:Colon / Q000187:drug effects* / Q000276:immunology; D019587:Dietary Supplements; D006498:Hepatectomy; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology; D007962:Leukocytes / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats
D007975:Leukotriene B4; D001120:Arginine