Construction of a cDNA microarray derived from the ascidian Ciona intestinalis.
A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain approximately 16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.
Azumi
Kaoru
K
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. kaoru@pharm.hokudai.ac.jp
Takahashi
Hiroki
H
Miki
Yasufumi
Y
Fujie
Manabu
M
Usami
Takeshi
T
Ishikawa
Hisayoshi
H
Kitayama
Atsusi
A
Satou
Yutaka
Y
Ueno
Naoto
N
Satoh
Nori
N
D000818:Animals; D002938:Ciona intestinalis / Q000196:embryology* / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D007814:Larva / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation; D015053:Zygote