In vitro toxicity testing of supramolecular sensitizers for photodynamic therapy.
We report the phototoxicity of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) and zinc metallocomplex (ZnTPPS4) sensitizers in the presence or absence of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on G361human melanoma cells. Morphological changes in cell cultures have been evaluated using inversion fluorescent microscope and image analysis. Viability of cells was determined by means of molecular probes for fluorescence microscopy (LIVE/DEAD kit- double staining with Calcein AM and Ethidium Homodimer). The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement with fluoroscan Ascent. We found that the most effective sensitizer is ZnTPPS4 bound to HP-beta-CD, since the IC50 value was 12.5 g/ml at the dose of light radiation of 10 J/cm2.
Kolárová
H
H
Department of Biophysics, Faculty of Medicine, Laboratory of Growth Regulators, Palacky University, Hnívotínská 3, 775 15 Olomouc, Czech Republic. kol@tunw.upol.cz
Mosinger
J
J
Lenobel
R
R
Kejlová
K
K
Jírová
D
D
Strnad
M
M
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007259:Infrared Rays; D020128:Inhibitory Concentration 50; D008027:Light; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D008856:Microscopy, Fluorescence; D010778:Photochemotherapy / Q000379:methods; D011166:Porphyrins / Q000633:toxicity*; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D018675:Toxicity Tests / Q000379:methods*
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C016136:tetraphenylporphine sulfonate