An efficient enzymatic synthesis of thiamin pyrophosphate.
Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14850, USA.
Jonathan S
JS
Melnick
K Ingrid
KI
Sprinz
Jason J
JJ
Reddick
Cynthia
C
Kinsland
Tadhg P
TP
Begley
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D017856:Phosphotransferases (Phosphate Group Acceptor); D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D013835:Thiamine Pyrophosphate / Q000138:chemical synthesis*
D017931:DNA Primers; D011994:Recombinant Proteins; D017856:Phosphotransferases (Phosphate Group Acceptor); C022735:thiamin-phosphate kinase; D013835:Thiamine Pyrophosphate