ELISA test for the detection of influenza H7 antibodies in avian sera.
Using a monoclonal antibody (MAb) specific for the H7 influenza surface glycoproteins, a serological enzyme-linked immunosorbent assay (ELISA) test has been developed. This MAb was made using the low-pathogenicity (LP) avian influenza (AI) strain (BS2676/99) isolated in Italy during a recent outbreak. The test is able to detect H7 antibodies in avian sera. The H7 ELISA has a 99% concordance of results with the classical hemagglutination inhibition (HI) test.
Istituto Zooprofilattico della Lombardia ed Emilia Romagna B.Ubertini Via A. Bianchi, 9-25124 Brescia, Italy.
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Sala
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Cordioli
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Moreno-Martin
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Tollis
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Brocchi
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Piccirillo
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Lavazza
D000482:Allantois / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D007558:Italy; D011200:Poultry; D015203:Reproducibility of Results; D014422:Turkeys
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus