Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay.
The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was >80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brasil.
Eleuza Rodrigues
ER
Machado
Marlene Tiduko
MT
Ueta
Maria do Rosário
Mdo R
de Fátima Gonçalves-Pires
João Batista
JB
Alves de Oliveira
Lúcia Helena
LH
Faccioli
Julia Maria
JM
Costa-Cruz
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D012377:Rodentia; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth