[Recombinant OspC identification and antigenicity detection from Borrelia burgdorferi PD91 in China].
OBJECTIVE
To recombine OspC gene from Borrelia burgdorferi PD91 of China and expressed it in E. coli for early diagnosis of Lyme disease.


METHODS
The OspC gene was amplified from the genome of Borrelia burgdorferi PD91 strain by polymerase chain reaction and recombined with plasmid PET-11D. The recombinant plasmid PET-11D-OspC was identified with PCR, restriction endonuclease analysis and sequencing. The antigenicity was verified with Western Blot.


RESULTS
OspC gene was cloned correctly into vector PET-11D. The resultant sequence was definitely different from the published sequence. The recombinant OspC seemed to have had strong antigenicity.


CONCLUSIONS
The findings laid basis for the studies on early diagnosis of Lyme disease.
Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Jian
J
Chen
Kang-Lin
KL
Wan
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C079616:OspC protein; D011994:Recombinant Proteins