Comparison of hexamethyldisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge.
We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
Araujo
Juliana C
JC
Departamento de Hidráulica e Saneamento, Universidade de São Paulo, Escola de Engenharia de São Carlos, Av. Trabalhador São Carlense 400, São Carlos-SP, 13566-590, Brazil. juli.calabria@ig.com.br
Téran
Francisco C
FC
Oliveira
Roberto A
RA
Nour
Edson A A
EA
Montenegro
Martha A P
MA
Campos
José R
JR
Vazoller
Rosana F
RF
D001421:Bacteria, Anaerobic / Q000648:ultrastructure*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D003890:Desiccation; D019640:Methanosarcinales / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D017646:Organosilicon Compounds; D012722:Sewage / Q000382:microbiology*; D016707:Tissue Fixation
D017646:Organosilicon Compounds; D012722:Sewage; C024548:hexamethylsilazane