A1 adenosine receptor knockout mice exhibit increased renal injury following ischemia and reperfusion.
Controversy exists regarding the effect of A1 adenosine receptor (AR) activation in the kidney during ischemia and reperfusion (I/R) injury. We sought to further characterize the role of A1 ARs in modulating renal function after I/R renal injury using both pharmacological and gene deletion approaches in mice. A1 AR knockout mice (A1KO) or their wild-type littermate controls (A1WT) were subjected to 30 min of renal ischemia. Some A1WT mice were subjected to 30 min of renal ischemia with or without pretreatment with 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) or 2-chrolo-cyclopentyladenosine (CCPA), selective A1 AR antagonist and agonist, respectively. Plasma creatinine and renal histology were compared 24 h after renal injury. A1KO mice exhibited significantly higher creatinines and worsened renal histology compared with A1WT controls following renal I/R injury. A1WT mice pretreated with the A1 AR antagonist or agonist demonstrated significantly worsened or improved renal function, respectively, after I/R injury. In addition, A1WT mice pretreated with DPCPX or CCPA showed significantly increased or reduced markers of renal inflammation, respectively (renal myeloperoxidase activity, renal tubular neutrophil infiltration, ICAM-1, TNF-alpha, and IL-1beta mRNA expression), while demonstrating no differences in indicators of apoptosis. In conclusion, we demonstrate that endogenous or exogenous preischemic activation of A1 ARs protects against renal I/R injury in vivo via mechanisms leading to decreased necrosis and inflammation.
Lee
H Thomas
HT
Department of Anesthesiology, College of Physicians and Surgeons of Columbia Univ., New York, NY 10032-3784, USA. tl128@columbia.edu
Xu
Hua
H
Nasr
Samih H
SH
Schnermann
Jurgen
J
Emala
Charles W
CW
D058186:Acute Kidney Injury / Q000276:immunology / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D058916:Adenosine A1 Receptor Antagonists; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D005838:Genotype; D020287:In Situ Nick-End Labeling; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D043682:Receptor, Adenosine A1 / Q000235:genetics*; D015427:Reperfusion Injury / Q000276:immunology / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014970:Xanthines / Q000494:pharmacology
D058916:Adenosine A1 Receptor Antagonists; D015415:Biomarkers; D007375:Interleukin-1; D012333:RNA, Messenger; D043682:Receptor, Adenosine A1; D014409:Tumor Necrosis Factor-alpha; D014970:Xanthines; D018799:Intercellular Adhesion Molecule-1; C058394:2-chloro-N(6)cyclopentyladenosine; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000241:Adenosine