DNA vaccine encoding pertussis toxin S1 subunit induces protection against Bordetella pertussis in mice.
Pertussis toxin (PT) is the major virulence factor of Bordetella pertussis, and detoxified PT is a crucial antigen of acellular pertussis vaccine. Here, plasmid DNA expressing the pertussis toxin S1 subunit (pcDNA/S1) of B. pertussis was evaluated for immunogenicity and for the ability to induce protection against PT challenge or B. pertussis infection in mice. The gene gun delivery of pcDNA/S1, performed by inserting the S1 gene into a mammalian expression vector, successfully induced anti-PT IgG antibody production. Immunization of mice with pcDNA/S1 significantly inhibited leukocytosis-promoting activity caused by PT or B. pertussis. In addition, pcDNA/S1 induced significant protection against intracerebral challenge with a lethal dose of B. pertussis. The results of the present study demonstrated that a DNA vaccine encoding the PT-S1 subunit induced protection against B. pertussis infection in mice. Thus, this vaccine preparation is potentially applicable for the production of novel vaccines against B. pertussis infection.
Kamachi
Kazunari
K
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan. kamachi@nih.go.jp
Konda
Toshifumi
T
Arakawa
Yoshichika
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000276:immunology; D005260:Female; D015151:Immunoblotting; D007964:Leukocytosis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D037342:Pertussis Toxin / Q000235:genetics* / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology*; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D018076:DNA, Complementary; D010567:Pertussis Vaccine; D019444:Vaccines, DNA; C083388:pertussis toxin, S1 subunit; D037342:Pertussis Toxin