Detection of bacteria by time-resolved laser-induced breakdown spectroscopy.
A laser-induced breakdown spectroscopy technique for analyzing biological matter for the detection of biological hazards is investigated. Eight species were considered in our experiment: six bacteria and two pollens in pellet form. The experimental setup is described, then a cumulative intensity ratio is proposed as a quantitative criterion because of its linearity and reproducibility. Time-resolved laser-induced breakdown spectroscopy (TRELIBS) exhibits a good ability to differentiate among all these species, whatever the culture medium, the species or the strain. Thus we expect that TRELIBS will be a good candidate for a sensor of hazards either on surfaces or in ambient air.
Direction Generale de l'Armement, Direction des Centres d'expertises et d'essais, Centre d'Etudes du Bouchet, B.P. 91710 Vert-Le-Petit, France.
Stéphane
S
Morel
Nicolas
N
Leone
Philippe
P
Adam
Jacques
J
Amouroux
D001419:Bacteria / Q000145:classification*; D003470:Culture Media; D004867:Equipment Design; D015386:Hazardous Substances / Q000032:analysis*; D007834:Lasers; D015203:Reproducibility of Results; D013045:Species Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*
D003470:Culture Media; D015386:Hazardous Substances