Degradation of phospholipid polymer hydrogel by hydrogen peroxide aiming at insulin release device.
The purpose of this study is to ascertain the applicable possibility of H(2)O(2) degradable hydrogel for fabrication of insulin release system synchronized with the change in the glucose concentration in the medium. The hydrogel was prepared by using 2-methacryloyloxyethyl phosphorylcholine (MPC) and crosslinker. The favorable characteristic of the hydrogel was H(2)O(2) concentration responsive degradation. The H(2)O(2) was utilized and produced by enzymatic reaction between glucose oxidase and glucose. Poly(MPC) (PMPC) was easily degraded in H(2)O(2) aqueous solution, and the PMPC hydrogel was also degraded in H(2)O(2) aqueous solution. The degradation mechanism was considered to be main chain scission of PMPC. The degradation profile was evaluated by using weight swelling ratio and volume swelling ratio. The weight swelling ratio of PMPC hydrogel firstly increased due to the reduction of crosslink density, then the ratio decreased to zero (complete degradation). The degradation profile was proportional to the H(2)O(2) concentration. Furthermore, volume swelling ratio also increased, and complex elastic modulus decreased with degradation in H(2)O(2) aqueous solution. These results indicated that the hydrogel was degraded by hydroxy and/or hydroperoxy radicals which was produced by H(2)O(2), the crosslink density and mechanical property decreased. The release profile from the hydrogel was estimated by using lipid microsphere (LM) as an insulin model. The LM was released with the degradation of PMPC hydrogel. Taking these results into account, the PMPC hydrogel was available for H(2)O(2) degradable hydrogel for synchronization with glucose concentration by using enzymatic reaction.
Uchiyama
Tomoaki
T
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Kiritoshi
Yoshihiro
Y
Watanabe
Junji
J
Ishihara
Kazuhiko
K
D020341:Absorbable Implants; D004058:Diffusion; D005240:Feasibility Studies; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry*; D007332:Insulin Infusion Systems; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D008970:Molecular Weight; D009997:Osmotic Pressure; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry*; D011109:Polymethacrylic Acids
D020100:Hydrogels; D007328:Insulin; D008081:Liposomes; D008567:Membranes, Artificial; D008689:Methacrylates; D011109:Polymethacrylic Acids; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; D006861:Hydrogen Peroxide