Ampicillin resistance in Listeria monocytogenes acquired as a result of transposon mutagenesis.
We have used plasmid pLTV3, which carries transposon Tn917, to obtain a series of mutants of Listeria monocytogenes EGD showing varied degrees of resistance to ampicillin and other beta-lactam antibiotics, including imipenem. In this paper we focus on the characteristics of two strains in which decreased susceptibility to ampicillin is accompanied by changes in the structure of the cell wall murein and cell-wall related changes of phenotype.
Department of Bacterial Physiology, Institute of Microbiology, Warsaw University, 02-096 Warsaw, Miecznikowa 1, Poland.
Joanna
J
Poroś-Głuchowska
Małgorzata
M
Kłoszewska
Zdzisław
Z
Markiewicz
D000667:Ampicillin / Q000494:pharmacology*; D000668:Ampicillin Resistance / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D004251:DNA Transposable Elements; D006602:Hexosyltransferases / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D008088:Listeriosis / Q000188:drug therapy; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism; D016254:Mutagenesis, Insertional; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000378:metabolism; D010458:Peptidyl Transferases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D000667:Ampicillin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase