[FasL-cDNA transfected into mouse bone marrow cells ex vivo to prevent graft versus host disease].
To explore the new approach to prevent graft versus host disease (GVHD) by purging ex vivo T lymphocytes of bone marrow graft through Fas-FasL way, FasL-cDNA was transfected into BALB/c mouse bon e marrow cells by liposome ex vivo. The transfected cells were cultured together with BAC (BALB/c x C57BL/6) mouse bone marrow graft. The mixing bone marrow graft was infused into BALB/c mouse recipients after 60Co-gamma irradiation. The mortality, manifestation and pathologic change of GVHD in recipient mice were observed. The CFU-S and Y chromosome from donor mice were detected. The results showed that compared with control group, the mortality in 60 days of the recipients in the experimental group decreased (20% vs 70%, P < 0.01) and the morbidity of GVHD lowered (40% vs 100%, P < 0.01). The CFU-S counts for all groups were at normal level on 20 days after transplantation. The Y chromosome from donor mice was discovered in 70% bone marrow nucleated cells of recipient mice survived over 2 months in the experimental group. It is concluded that mFasL-cDNA transfected mouse bone marrow cells prevent GVHD after culturing together with bone marrow graft, and accelerate hematopoietic reconstitution in recipient mice.
Department of Pediatrics, People's Hospital, Wuhan University, Wuhan 430060, China.
Zhi-Liang
ZL
Xu
Ping
P
Zou
Ling-Bo
LB
Liu
Ai-Xiang
AX
Li
Yan-Ping
YP
Ma
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D053222:Fas Ligand Protein; D005260:Female; D015316:Genetic Therapy; D006086:Graft vs Host Disease; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins