7, 8-dihydroxyflavanone as an inhibitor for Jun-Fos-DNA complex formation and its cytotoxic effect on cultured human cancer cells.
7,8-Dihydroxyflavanone, isolated from the seeds of Alpinia Katsumadai Hayata, showed an inhibitory effect on Jun-Fos dimer action. 7,8-Dihydroxyflavanone blocked the action of the dimer on a DNA consensus sequence, the AP-1 binding site. We have concluded that the Jun-Fos heterodimer, bound with 7,8-dihydroxyflavanone, cannot bind to the AP-1 site and therefore results in signal interruption. The 7,8-dihydroxyflavanone was also found to have an in vitro cytotoxic effect against A549 (a human lung cancer cell line) and K562 (a human leukemia cell line).
School of Chemistry and Molecular Engineering, College of Natural Sciences, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul, 151-742, Korea.
Eun-Ryeong
ER
Hahm
Seyeon
S
Park
Chul-Hak
CH
Yang
D032429:Alpinia / Q000737:chemistry*; D018736:DNA Adducts; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D016762:Genes, fos / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics*; D007938:Leukemia / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
C478869:7,8-dihydroxyflavanone; D018736:DNA Adducts; D044950:Flavanones; D018808:Transcription Factor AP-1