[Study on autoimmune thrombocytopenic purpura related antibody].
OBJECTIVE
To investigate a specific and sensitive assay for the diagnosis of autoimmune thrombocytopenic purpura (AITP).


METHODS
Glycoprotein specific autoantibodies in platelet eluate and plasma were detected by a modified monoclonal antibody immobilization of platelet antigens assay (MAIPA).


RESULTS
The overall positive rate of specific autoantibodies against platelet GPIIb/IIIa and GPIb/IX in plasma was 38.89% and in eluated platelet membrane was 68.52%. The difference between them was significant (corrected chi(2) = 19.39, P < 0.005). The proportion of positive MAIPA results between primary AITP and secondary AITP was not significantly different. There was a significant inverse correlation between antibody level and platelet count.


CONCLUSIONS
Detection of eluated GP-specific autoantibodies by MAIPA is highly specific and much more sensitive as compared with the measurement of their plasma counterparts in the diagnosing and therapeutic monitoring of AITP.
Lu
Lu
L
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Hou
Ming
M
Shi
Yan
Y
Qin
Ping
P
Peng
Jun
J
Zhu
Yuan-yuan
YY
Zhang
Mao-hong
MH
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*
D001323:Autoantibodies; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex