Comparison of medulloblastoma and normal neural transcriptomes identifies a restricted set of activated genes.
Over 1.4 million transcript tags expressed in 20 different human medulloblastomas were counted using serial analysis of gene expression. Digital gene expression profiles in the medulloblastoma were compared to multiple regions of the normal human brain, revealing 30 transcripts with high expression in multiple tumors and little or no expression in the normal cerebellum and other adult and pediatric brain regions. Using independent medulloblastoma samples and normal tissue, real-time PCR verified eight of nine selected genes as candidate tumor-associated antigens. Differential protein expression for CD24, prolactin and Topo2A was further confirmed by immunohistochemical analysis using medulloblastoma and normal brain sections and a tissue microarray. The genes highly expressed in the medulloblastoma include PRAME, a cancer-testis antigen and potential targets for immunotherapy.
Department of Pathology, Duke University Medical Center, Box 3156, Durham NC 27710, USA.
Kathy
K
Boon
Jennifer B
JB
Edwards
I-Mei
IM
Siu
Deric
D
Olschner
Charles G
CG
Eberhart
Marco A
MA
Marra
Robert L
RL
Strausberg
Gregory J
GJ
Riggins
D001921:Brain / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008527:Medulloblastoma / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D012334:RNA, Neoplasm