[Effect of eosin Y on Ca2+, Mg2+-ATPase of the smooth muscle sarcolemma].
Eosin Y was studied with the aim to elucidate the mechanism of its inhibitory effect on the activity of Ca(2+)-transporting ATPase of myometrium cell plasma membrane. The inhibitor was studied for its effect on the maximal rate of the ATP-hydrolase reaction catalyzed by Ca2+, Mg(2+)-ATPase, on the enzyme affinity for the substrate and a possibility of enzyme activity protection under the inhibitor effect by the main reagents of ATP-hydrolase reaction. It was established that eosin Y decreased the turnover rate of this enzyme and his affinity for ATP. Preincubation of ATPase with ATP (or ATP plus MgCl2) had no effect on the extent of enzyme inhibition by eosin Y. This result proves that eosin Y and ATP do not compete for the site of binding on the enzyme.
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. slinchenko@biochem.kiev.ua
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Slinchenko
I H
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Chernysh
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Kosterin
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D009130:Muscle, Smooth / Q000201:enzymology*; D009215:Myometrium / Q000201:enzymology; D012508:Sarcolemma / Q000201:enzymology*
D004791:Enzyme Inhibitors; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D004801:Eosine Yellowish-(YS)