A novel type of VP4 carried by a porcine rotavirus strain.
The gene encoding the VP8* trypsin-cleavage product of the VP4 protein of porcine rotavirus strain A34 was sequenced, and the predicted amino acid (aa) sequence was compared to the homologous region of all known P genotypes. The aa sequence of the VP8* of strain A34 shared low identity, ranging from 39% (bovine strain B223, P8[11]) to 76% (human strain 69M, P4[10]), with the homologous sequences of representative strains of the remaining 21 P genotypes. Phylogenetic relationships showed that the VP8* of strain A34 shares a common evolutionary lineage with those of human 69M (P4[10]) and equine H-2 (P4[12]) strains. Hyperimmune sera raised to strain A34 and to a genetic reassortant strain containing the VP4 gene from strain A34, both with high homologous neutralization titer via VP4, failed to neutralize strains representative of 15 different P genotypes. These results indicate that strain A34 should be considered as prototype of a new P genotype and serotype (P14[23]) and provide further evidence for the vast genetic and antigenic diversity of group A rotaviruses.
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, 21827 Caracas 1020-A, Venezuela. fliprand@psateur.ivic.ve
Ferdinando
F
Liprandi
Marlene
M
Gerder
Zoleida
Z
Bastidas
José A
JA
López
Flor H
FH
Pujol
Juan E
JE
Ludert
Daniel B
DB
Joelsson
Max
M
Ciarlet
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification*; D012703:Serotyping; D013552:Swine / Q000821:virology*
D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus