[Detection of the DeltaF508 trinucleotide deletion in the human CFTR gene by UV immobilization on nylon of a complex of a PCR fragment with a highly specific probe].
Deletion delta F508 has been revealed in PCR-amplified regions of human gene CFTR by color detection of the hybridization complex obtained by ligation of a tandem of short oligonucleotides on a DNA template followed by UV immobilization on nylon. The method allows reliable detection of the three-nucleotide deletion (insertion). The nonspecific signal depends on the nucleotide composition of the biotinylated tandem component. A significant level of the specific signal was achieved by using the PCR-amplified DNA fragments of different length (200-400 bp) irrespective of the position of the tandem-binding site in their sequences.
Novosiberian Institute of Bioorganic Chemistry, Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
M R
MR
Kabilov
D V
DV
Pyshnyĭ
G M
GM
Dymshits
V F
VF
Zarytova
E M
EM
Ivanova
D001483:Base Sequence; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D017931:DNA Primers; D015342:DNA Probes; D006801:Humans; D009693:Nucleic Acid Hybridization; D009757:Nylons; D017384:Sequence Deletion; D014466:Ultraviolet Rays
C505032:CFTR protein, human; D017931:DNA Primers; D015342:DNA Probes; D009757:Nylons; D019005:Cystic Fibrosis Transmembrane Conductance Regulator