Construction of a cDNA microarray derived from the ascidian Ciona intestinalis.
A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain approximately 16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. kaoru@pharm.hokudai.ac.jp
Kaoru
K
Azumi
Hiroki
H
Takahashi
Yasufumi
Y
Miki
Manabu
M
Fujie
Takeshi
T
Usami
Hisayoshi
H
Ishikawa
Atsusi
A
Kitayama
Yutaka
Y
Satou
Naoto
N
Ueno
Nori
N
Satoh
D000818:Animals; D002938:Ciona intestinalis / Q000196:embryology* / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D007814:Larva / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation; D015053:Zygote