Glial cell and fibroblast cytotoxicity study on plasma-deposited diamond-like carbon coatings.
Diamond-like carbon films have been evaluated as coatings to improve biocompatibility of orthopedic and cardiovascular implants. This study initiates a series of investigations that will evaluate diamond-like carbon (DLC) as a coating for improved biocompatibility in chronic neuroprosthetic implants. Studies in this report assess the cytotoxicity and cell adhesion behavior of DLC coatings exposed to glial and fibroblast cell lines in vitro. It can be concluded from these studies that DLC coatings do not adversely affect 3T3 fibroblast and T98-G glial cell function in vitro. We also successfully rendered DLC coatings non-adhesive (no significant fibroblast or glial cell adhesion) with surface immobilized dextran using methods developed for other biomaterials and applications. Future work will further develop DLC coatings on prototype microelectrode devices for chronic neural implant applications.
Harrington Department of Bioengineering, Arizona State University, PO Box 879709, Tempe, AZ 85287-9709, USA.
Amarjit
A
Singh
Gholamreza
G
Ehteshami
Stephen
S
Massia
Jiping
J
He
Robin G
RG
Storer
Gregory
G
Raupp
D016475:3T3 Cells; D000818:Animals; D002244:Carbon; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D018130:Diamond; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006358:Hot Temperature; D008422:Materials Testing; D051379:Mice; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology*
D020099:Coated Materials, Biocompatible; D002244:Carbon; D018130:Diamond