Ion release from copper phosphate cement and influence on Streptococcus mutans growth in vitro: a comparative study.
The aim of this study was to compare the effects of a black copper cement (BCC), an established restorative material (a conventional glass ionomer cement) and two temporary restorative materials (a zinc phosphate and a zinc polycarboxylate cement) on the growth of Streptococcus mutans in vitro, and to correlate bacterial growth with ion release from each material. Test specimens were eluted in either 0.1 M lactic acid, pH 4, or 0.1 M sodium chloride, pH 7. At 2 days, 7 days, 28 days and 6 months, eluates were inoculated with S. mutans and bacterial growth was recorded. Metal ion (Cu(2+), Zn(2+ )and Mg(2+)) and fluoride release were measured. At most immersion times, the different materials had a statistically significant inhibitory effect on bacterial growth compared to the respective control, at both pH levels. The inhibitory effect decreased with time and in most cases was associated with high levels of ion release at the beginning of the experimental period, followed by significantly lower levels. For BCC, there were statistically significant relationships between the median rates of growth of S. mutans in the presence of BCC eluates and the median values for release of copper and zinc, although not magnesium. Of the different materials, BCC demonstrated greatest antibacterial activity.
Unit of Dental and Oral Health, Dundee Dental Hospital, Dundee, UK. j.foley@dundee.ac.uk
Jennifer
J
Foley
Alison
A
Blackwell
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology*; D005459:Fluorides / Q000737:chemistry; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D011076:Polycarboxylate Cement / Q000737:chemistry / Q000494:pharmacology; D012965:Sodium Chloride / Q000737:chemistry; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors; D015032:Zinc / Q000737:chemistry; D015036:Zinc Phosphate Cement / Q000737:chemistry / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003738:Dental Cements; D005899:Glass Ionomer Cements; D011076:Polycarboxylate Cement; C031354:black copper cement; D019344:Lactic Acid; D012965:Sodium Chloride; D015036:Zinc Phosphate Cement; D003300:Copper; D008274:Magnesium; D015032:Zinc; D005459:Fluorides