[Effect of ethanol on structural and functional characteristics of rat brain cortical membranes in vitro].
When finding the mechanism of the in vitro ethanol membrane action it was established that alcohol in moderate concentrations enhanced the inactivation of the rat brain cortex Na+, K(+)-ATPase after modification of the microsomal fraction by phospholipase A2 from cobra venom. It is supposed that ethanol sensitivity is determined by the structural state of the functional protein-lipid enzyme complexes in membrane. The decrease of the intrinsic tryptophan fluorescence of the membrane proteins and fluorescence of the probe 1-anilinonaphthalene-8-sulfonate (both without and under membrane surface charge shielding) in the presence of ethanol was revealed. It is concluded, that conformational changes of the membrane polar surface area coincide with the enzyme inactivation by ethanol.
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
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Mishchuk
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Kaplia
D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010740:Phospholipases / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D000431:Ethanol; C027132:1-anilino-8-naphthalenesulfonate; D010740:Phospholipases; D000254:Sodium-Potassium-Exchanging ATPase