[Revised prevalence of afa+ Escherichia coli strains in acute pyelonephritis of children].
Two hundred E. coli strains isolated from children with pyelonephritis were investigated for the presence of six virulence factors. The used primers amplified adhesin pap and sfa, toxin haemolysin (hly) and cytotoxic necrotizing factor 1 (cnf1) and aerobactin (aer). For afimbrial adhesin, the previously used set of primers could not allow to detect the newly reported afa operons (Le Bouguenec et al., 2001). With a new set of primers specific for the afa operon family the prevalence of afa+ strains increased from 3.5% to 13.5%. Combinations of three or more factors in a same strain were found in 48.5%. Thirty two different urovirulent genotypes were observed; two strains contained the six studied factors.
Laboratoire de microbiologie et génétique moléculaires, IBCG, CNRS, université Paul-Sabatier, 118, route de Narbonne, 31062 cedex Toulouse, France.
P
P
Licznar
I
I
Eychenne
C
C
Azéma
S
S
Decramer
F
F
Bouissou
O
O
Fayet
M-F
MF
Prère
D018830:Adhesins, Escherichia coli / Q000235:genetics; D000900:Anti-Bacterial Agents; D002648:Child; D004359:Drug Therapy, Combination / Q000627:therapeutic use*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000453:epidemiology*; D005838:Genotype; D006801:Humans; D009876:Operon; D011704:Pyelonephritis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D014774:Virulence
D018830:Adhesins, Escherichia coli; D000900:Anti-Bacterial Agents