Estrogenic compounds suppressed interferon-gamma production in mouse splenocytes through direct cell-cell interaction.
Here, we reported the effects of 17beta-estradiol (E2), isoflavone genistein (Gen), and daidzein (Dai) on the production of interferon (IFN)-gamma by splenocytes isolated from C57BL/6N mice. When mouse splenocytes were stimulated with lipopolysaccharide, E2, Gen, and Dai suppressed the production of IFN-gamma. However, when only nonadherent cell populations of splenocytes were tested, none of these estrogenic compounds suppressed IFN-gamma production. This result indicates that IFN-gamma production by nonadherent cell populations of splenocytes treated with estrogens is regulated by adherent cell populations. Moreover, direct cell-cell interaction between both populations was necessary for suppression of IFN-gamma production by nonadherent populations. In addition, E2 conjugated with bovine serum albumin inhibited IFN-gamma production as well as E2. This result suggests that the plasma membrane-associated estrogen receptor plays a prominent role in this suppression mechanism.
Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. makorenu@agr.kyushu-u.ac.jp
Mako
M
Nakaya
Masao
M
Yamasaki
Yoshiyuki
Y
Miyazaki
Hirofumi
H
Tachibana
Koji
K
Yamada
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D000077267:Fulvestrant; D019833:Genistein / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D007529:Isoflavones / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011960:Receptors, Estrogen / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000187:drug effects*
D004965:Estrogen Antagonists; D004967:Estrogens; D007529:Isoflavones; D011960:Receptors, Estrogen; D000077267:Fulvestrant; D004958:Estradiol; C004742:daidzein; D007371:Interferon-gamma; D019833:Genistein