PTTH-stimulated ecdysone secretion is dependent upon tyrosine phosphorylation in the prothoracic glands of Manduca sexta.
PTTH stimulates ecdysteroid secretion by the insect prothoracic glands. The peptide activates cAMP synthesis in a calcium-dependent manner, ultimately enhancing ecdysteroid synthesis. We have found that PTTH stimulates a rapid increase in tyrosine phosphorylation of at least four proteins in the prothoracic glands of larval Manduca sexta, as seen on Western blots of glandular lysates probed with antibody directed against phosphotyrosine. PTTH-stimulated tyrosine phosphorylation is blocked by an inhibitor of Src family tyrosine kinases, 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]-pyrimidine (PP1). The inhibitor also blocks PTTH-stimulated ecdysone secretion, as well as PTTH-stimulated cAMP synthesis. Direct activation of the catalytic subunit of adenylyl cyclase by forskolin is not affected by PP1. In addition, ecdysteroid secretion stimulated by the cAMP analog dbcAMP is not blocked by PP1. These findings point to an important role for a Src-family tyrosine kinase at a very early step in the PTTH signaling pathway, prior to the activation of adenylyl cyclase.
Department of Biology, Northeastern University, 433 Richards Hall, 360 Huntington Avenue, Boston, MA 02115, USA. w.smith@neu.edu
Wendy
W
Smith
Jennifer
J
Priester
Jason
J
Morais
D000818:Animals; D015153:Blotting, Western; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis / Q000494:pharmacology; D004440:Ecdysone / Q000378:metabolism*; D004702:Endocrine Glands / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007301:Insect Hormones / Q000378:metabolism* / Q000494:pharmacology*; D007814:Larva; D018402:Manduca / Q000378:metabolism*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*; D019061:src-Family Kinases / Q000037:antagonists & inhibitors
C108123:4-amino-5-(4-methylphenyl)-7-(tert-butyl)pyrazolo(3,4-d)pyrimidine; D004791:Enzyme Inhibitors; D007301:Insect Hormones; D011720:Pyrazoles; D011743:Pyrimidines; D005576:Colforsin; D004440:Ecdysone; D014443:Tyrosine; C009089:prothoracicotropic hormone; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D019061:src-Family Kinases