Transcriptional activation of mouse mast cell protease-9 by microphthalmia-associated transcription factor.
We explored transcriptional regulation of mouse mast cell protease-9 (mMCP-9), which is implicated in inflammation of the jejunum during helminth infections and tissue remodeling of the uterus during pregnancy. Transcription was positively regulated by microphthalmia-associated transcription factor (MITF), a member of the basic helix-loop-helix-leucine zipper family that binds to the E-box, a CANNTG sequence. The most significant segment for positive regulation by MITF was nt -183 to -177 of the mMCP-9 promoter, CATCATG, which bound MITF-M. In addition, not only other MITF isoforms but also TFE3, another member of the family, activated mMCP-9 transcription through this nucleotide sequence inserted one base within the E-box.
Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara, Japan.
Masaru
M
Murakami
Teruo
T
Ikeda
Kenji
K
Ogawa
Masayuki
M
Funaba
D020816:Amino Acid Motifs; D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000201:enzymology*; D008546:Melanoma, Experimental / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor; D011485:Protein Binding; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
D004268:DNA-Binding Proteins; C497234:MITF protein, human; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D014157:Transcription Factors; C109138:Mcpt9 protein, mouse; D012697:Serine Endopeptidases