Fibroblast cells: a sensing bioelement for glucose detection by impedance spectroscopy.
Modifying the electrical properties of fibroblasts against various glucose concentrations can serve as a basis for a new, original sensing device. The aim of the present study is to test a new biosensor based on impedancemetry measurement using eukaryote cells. Fibroblast cells were grown on a small optically transparent indium tin oxide semiconductor electrode. Electrochemical impedance spectroscopy (EIS) was used to measure the effect of D-glucose on the electrical properties of fibroblast cells. Further analyses of the EIS results were performed using equivalent circuits in order to model the electrical flow through the interface. The linear calibration curve was established in the range 0-14 mM. The specification of the biosensors was verified using cytochalasin B as an inhibitor agent of the glucose transporters. The nonreactivity to sugars other than glucose was demonstrated. Such a biosensor could be applied to a more fundamental study of cell metabolism.
Laboratoire d'Ingénierie et de Fonctionnalisation des Surfaces, UMR-CNRS 5621, ECL-Lyon, 36 Avenue Guy de Collongues, 69134 Ecully Cedex, France. chaker_tlili@yahoo.fr
Chaker
C
Tlili
Karine
K
Reybier
Alain
A
Géloën
Laurence
L
Ponsonnet
Claude
C
Martelet
Hafedh Ben
HB
Ouada
Michel
M
Lagarde
Nicole
N
Jaffrezic-Renault
D016475:3T3 Cells; D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D003470:Culture Media / Q000737:chemistry; D004563:Electrochemistry; D005347:Fibroblasts / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D051379:Mice
D003470:Culture Media; D005947:Glucose