[Role of innate macrophages in DNA and protein biosynthesis in cultured cells of murine (line A) ascitic hepatoma].
Joint effect of high-density lipoproteins (HDLP) and glucocorticoids presence is responsible for increased protein and DNA biosynthesis in the cells of the hepatic parenchyme. Hepatic innate macrophages devour HDLP and glucocorticoids and release the A-1 tetrahydrocorisol-apolipoprotein complex which in turn is channeled into interstitial spaces to be captured by hepatocytes, thus stimulating protein and DNA biosynthesis. This mechanism was observed in cultured cells of murine ascitic hepatoma; it was regulated by macrophages present in incubation medium. The rate of protein and DNA biosynthesis remained constant unless HDLP and glucocorticoids were present in the medium and in a macrophage-containing culture alone.
Research Institute of Biochemistry, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk.
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Panin
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Khoshchenko
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D005938:Glucocorticoids / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005938:Glucocorticoids; D008075:Lipoproteins, HDL; D009363:Neoplasm Proteins