Shape-adaptable water-soluble conjugated polymers.
Cationic water-soluble conjugated polymers with a non "rigid-rod" aspect ratio can be prepared by Suzuki coupling condensation polymerization of 2,7-dibromo-9,9-bis(6'-bromohexyl)fluorene and varying ratios of p- and m-phenyl diboronic acids, followed by reaction with trimethylamine. The resulting polymers are designated MnPm+, where the subscripts in Mn and Pm correspond to the ratio of meta and para linkages, respectively. Absorption and fluorescence spectra indicate facile energy transfer via interachain or intrachain mechanisms. The emission of MnPm+ with m > 50 matches that of M0P100+. Excitations ultimately reside in the polymer segments with largest number of para linkages. FRET experiments show that the polymers with a more flexible structure are better donors to fluorescein-labeled double-stranded DNA. Similar results are obtained when intercalated ethidium bromide is used as the acceptor. These results indicate that there is better spatial registry between the flexible conjugated polymers and the secondary structure of DNA.
Department of Chemistry, Institute for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.
Bin
B
Liu
Shu
S
Wang
Guillermo C
GC
Bazan
Alexander
A
Mikhailovsky
D002412:Cations; D004247:DNA / Q000737:chemistry; D004735:Energy Transfer; D019793:Fluorescein / Q000737:chemistry; D008163:Luminescent Measurements; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry
D002412:Cations; D011108:Polymers; D014867:Water; D004247:DNA; D019793:Fluorescein