[A simple and highly efficient Agrobacterium-mediated rice transformation system].
A simple and highly efficient rice transformation system was established based on the studying of factors influencing the Agrobacterium-mediated rice transformation. Embryogenic calli derived from mature embryos were infected and cocultivated with A. tumefaciens EHA101 harboring binary vectors: pHQ9, pHQ10, pHQT3. The highest transformation frequency was about 100 hygromycin resistance calli per gram of calli explants, and above 85% of these calli could be regenerated into plants. The putative transgenic plants were confirmed by GUS assay and southern-blot analysis. Hygromycin resistance tests indicated that the segregation of transgene in T1 progeny corresponded to the Mendelian ratio, 3:1. This system will benefit the functional genomic study of rice by using T-DNA insertion mutagenesis and gene targeting.
Li
Mei Ru
MR
South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650.
Li
Hong Qing
HQ
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002934:Cinnamates / Q000494:pharmacology; D004351:Drug Resistance; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A