Piezo electric sensor for endocrine-disrupting chemicals using receptor-co-factor interaction.
In vitro screening assays are useful techniques for the determination of receptor-mediated activities in environmental samples. In order to define whether environmental chemicals act as an agonist or antagonist to the human estrogen receptor (hER), we have constructed a biosensor based on ligand-inducible interactions between hER and relative proteins on a quartz crystal microbalance (QCM). The his-tagged proteins, which were expressed in E. coli by recombinant DNA technology, were immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified iminodiacetic acid. The resonance-frequency change of the protein-modified electrode was caused by association or dissociation with the hER relative proteins on the surface in the presence of estrogen. These results suggest that this sensor is applicable as a large-scale screening tool for estrogenic compounds.
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Masaharu
M
Murata
Chifumi
C
Gouda
Kentaro
K
Yano
Shinichiro
S
Kuroki
Tatsuo
T
Suzutani
Yoshiki
Y
Katayama
D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis*; D051548:Histone Acetyltransferases; D006801:Humans; D008024:Ligands; D056920:Nuclear Receptor Coactivator 1; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000819:agonists / Q000037:antagonists & inhibitors / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry
D004785:Environmental Pollutants; D008024:Ligands; D011960:Receptors, Estrogen; D014157:Transcription Factors; D051548:Histone Acetyltransferases; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1