Correlation between pyrazinamide activity and pncA mutations in Mycobacterium tuberculosis isolates in Taiwan.
A total of 76 clinical Mycobacterium tuberculosis isolates from Taiwan were tested for pyrazinamidase activity, pyrazinamide susceptibility, and pncA mutations. Frequency of resistance to PZA rose with increases in resistance to first-line drugs. Of 17 pyrazinamide-resistant strains, 7 (3 of which had not been previously described) possessed mutations in the pncA gene.
Section of Microbiology and Infectious Diseases, Kaohsiung Veterans General Hospital and Department of Medical Technology, Foo-Yin Institute of Technology, Taipei, Taiwan.
Tsi-Shu
TS
Huang
Susan Shin-Jung
SS
Lee
Hui-Zin
HZ
Tu
Wen-Kuei
WK
Huang
Yao-Shen
YS
Chen
Chung-Kai
CK
Huang
Shue-Ren
SR
Wann
Hsi-Hsun
HH
Lin
Yung-Ching
YC
Liu
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D000995:Antitubercular Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005838:Genotype; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D011718:Pyrazinamide / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan; D014376:Tuberculosis / Q000382:microbiology*
D000995:Antitubercular Agents; D004269:DNA, Bacterial; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis; C022490:pyrazinamide deamidase