Analysis of protein interactions using fluorescence technologies.
Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.
Yan
Yuling
Y
Department of Physiology, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA.
Marriott
Gerard
G
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020543:Proteome
D005456:Fluorescent Dyes; D011506:Proteins; D020543:Proteome