[Effects of shouwu-huanjing recipe on human sperm motility and fertility in vitro].
OBJECTIVE
To observe the effect of Shouwu-Huanjing Recipe(SWHJR) medicated serum on human sperm motility and fertility in vitro.


METHODS
Human sperm was co-cultured with SWHJR medicated serum in vitro. Human sperm motility was evaluated by computer-assisted semen analysis(CASA). The acrosome reaction and the capability of penetrating zona-free hamster eggs were also observed.


RESULTS
The co-cultured SWHJR medicated serum significantly increased the sperm motion velocity(VAP, VCL, VSL) (P < 0.01), the amplitude of lateral head movement (ALH) and the beat frequency of flagellum(BCF), the density of progressive motility sperms (P < 0.05), the acrosome reaction rate(P < 0.001), the fertilization rate(FR) and the fertilization index (FI) in sperm penetration assay(SPA) test (P < 0.01). The stimulation of SWHJR medicated serum occurred in dose-dependent manner.


CONCLUSIONS
SWHJR can improve human sperm motility and fertility.
Zeng
Jin-Xiong
JX
Department of Andrology & TCM, Fuzhou Dongfang Hospital, Fuzhou, Fujian 350025, China. zjxtcm@sina.com
Dai
Xi-Hu
XH
Liu
Jian-Hua
JH
Yang
Jia-Hui
JH
Chen
Jin-Feng
JF
Lin
Mu-Nan
MN
D020101:Acrosome Reaction; D000818:Animals; D006224:Cricetinae; D005298:Fertility / Q000187:drug effects*; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008647:Mesocricetus; D051381:Rats; D017207:Rats, Sprague-Dawley; D013081:Sperm Motility / Q000187:drug effects*