Different effects of astrocytes and Schwann cells on regenerating retinal axons.
Following a crush injury of the optic nerve in adult rats, the axons of retinal ganglion cells, stimulated to regenerate by a lens injury and growing within the optic nerve, are associated predominantly with astrocytes: they remain of small diameter (0.1-0.5 microm) and unmyelinated for > or = 2 months after the operation. In contrast, when the optic nerve is cut and a segment of a peripheral nerve is grafted to the ocular stump of the optic nerve, the regenerating retinal axons are associated predominantly with Schwann cells: they are of larger diameter than in the previous experiment and include unmyelinated axons (0.2-2.5 microm) and myelinated axons (mean diameter 2.3 microm). Thus, the grafted peripheral nerve, and presumably its Schwann cells, stimulate enlargement of the regenerating retinal axons leading to partial myelination, whereas the injured optic nerve itself, and presumably its astrocytes, does not. The result points to a marked difference of peripheral (Schwann cells) and central (astrocytes) glia in their effect on regenerating retinal axons.
Campbell
Gregor
G
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WCIE 6BT, UK. g.cambell@ucl.ac.uk
Kitching
Juliet
J
Anderson
Patrick N
PN
Lieberman
A Robert
AR
D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000648:ultrastructure; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D016380:Brain Tissue Transplantation; D005260:Female; D006085:Graft Survival / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D009409:Nerve Crush; D009413:Nerve Fibers, Myelinated / Q000502:physiology / Q000648:ultrastructure; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D009900:Optic Nerve / Q000254:growth & development / Q000601:surgery / Q000648:ultrastructure; D020221:Optic Nerve Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D010525:Peripheral Nerves / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000502:physiology* / Q000648:ultrastructure; D012583:Schwann Cells / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure