Modulations of food-derived substances on intestinal permeability in Caco-2 cell monolayers.
The effects of more than 300 kinds of food extracts on intestinal permeability were investigated in Caco-2 cells with the use of model compounds: Lucifer Yellow (LY) for the paracellular pathway, Fluorescein (FC) for the monocarboxylic acid transporter-mediated pathway, and Rhodamine 123 (RH) for the p-glycoprotein-mediated efflux pathway. With several extracts of increasing or decreasing LY permeation, increasing FC or RH permeation was also observed, indicating modulation by dietary substances in several pathways for intestinal absorption.
Applied Bioresearch Center, Research & Development, Department, Kirin Brewery Co., Ltd., Takasaki-shi, Gunma, Japan. konishiy@kirin.co.jp
Yutaka
Y
Konishi
D018938:Caco-2 Cells; D019069:Cell Respiration; D002470:Cell Survival; D004396:Coloring Agents / Q000493:pharmacokinetics; D005502:Food; D006801:Humans; D007408:Intestinal Absorption; D007422:Intestines; D010539:Permeability
D004396:Coloring Agents