Proteome of Haemophilus ducreyi by 2-D SDS-PAGE and mass spectrometry: strain variation, virulence, and carbohydrate expression.
We have analyzed the proteome of several strains of Haemophilus ducreyi by two-dimensional gel electrophoresis (2-DE) and mass spectrometry. Over 100 spots were analyzed from the soluble and insoluble protein fractions from the prototype strain 35000HP and 122 distinct proteins were identified. Functions of approximately 80% of the 122 proteins were deduced by identification with close homologues of Haemophilus influenzae. Four additional wild type and three mutant strains were also analyzed that vary in their virulence and/or outer-membrane lipooligosaccharide structures. Overall, the 2-DE gel maps of the wild type and mutant strains were similar to strain 35000HP, suggesting little proteome diversity in relation to carbohydrate expression and/or virulence. An exception was the Kenyan strain 33921 which contained significant differences in its proteome 2-DE map and also synthesizes an unusual LOS with a trisaccharide branch structure. This African strain may represent a prototype of a second clonal group of H. ducreyi.
Buck Institute for Age Research, Novato, California 94945, USA.
N Karoline
NK
Scheffler
Arnold M
AM
Falick
Steven C
SC
Hall
William C
WC
Ray
Deborah M
DM
Post
Robert S
RS
Munson
Bradford W
BW
Gibson
D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017353:Gene Deletion; D005798:Genes, Bacterial; D014644:Genetic Variation; D016680:Genome, Bacterial; D006191:Haemophilus ducreyi / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006193:Haemophilus influenzae / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D014774:Virulence
D008070:Lipopolysaccharides; D020543:Proteome