A general statistical framework for mapping quantitative trait loci in nonmodel systems: issue for characterizing linkage phases.
Because of uncertainty about linkage phases of founders, linkage mapping in nonmodel, outcrossing systems using molecular markers presents one of the major statistical challenges in genetic research. In this article, we devise a statistical method for mapping QTL affecting a complex trait by incorporating all possible QTL-marker linkage phases within a mapping framework. The advantage of this model is the simultaneous estimation of linkage phases and QTL location and effect parameters. These estimates are obtained through maximum-likelihood methods implemented with the EM algorithm. Extensive simulation studies are performed to investigate the statistical properties of our model. In a case study from a forest tree, this model has successfully identified a significant QTL affecting wood density. Also, the probability of the linkage phase between this QTL and its flanking markers is estimated. The implications of our model and its extension to more general circumstances are discussed.
Lin
Min
M
Department of Statistics, University of Florida, Gainesville, Florida 32611, USA.
Lou
Xiang-Yang
XY
Chang
Myron
M
Wu
Rongling
R
D002874:Chromosome Mapping / Q000379:methods* / Q000706:statistics & numerical data; D003627:Data Interpretation, Statistical; D008040:Genetic Linkage; D006824:Hybridization, Genetic; D008962:Models, Theoretical; D009010:Monte Carlo Method; D032107:Populus / Q000235:genetics; D040641:Quantitative Trait Loci