Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries.
The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 micromol ml(-1), showed a maximum capacity of 9.1 mg Mab ml(-1) and a dynamic capacity of 3.9 mg Mab ml(-1). A 95% yield of electrophoretically pure anti-GM-CSF was obtained in a single step.
Facultad Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (1113) Buenos Aires, Argentina.
S A
SA
Camperi
N B
NB
Iannucci
G J
GJ
Albanesi
M
M
Oggero Eberhardt
M
M
Etcheverrigaray
A
A
Messeguer
F
F
Albericio
O
O
Cascone
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry* / Q000276:immunology; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor