Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures.
A complementary DNA encoding human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned and introduced into tomato (Lycopersicon esculentum cv. Seokwang) using Agrobacterium-mediated transformation. Genomic PCR and Northern blot analysis demonstrated the integration of the construction into the plant nuclear genome and expression of the hGM-CSF in transgenic tomato. The cell suspension culture was established from leaf-derived calli of the transgenic tomato plants transformed with the hGM-CSF gene. Recombinant hGM-CSF was synthesized by the transgenic cell culture and secreted into the growth medium at 45 microg l(-1) after 10 d' cultivation.
Kwon
Tae-Ho
TH
Basic Science Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Kim
Young-Sook
YS
Lee
Jae-Hwa
JH
Yang
Moon-Sik
MS
D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014018:Tissue Distribution
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor