A preliminary linkage map of the tick, Ixodes scapularis.
A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximately 10(9) bp, the relationship of physical to genetic distance is approximately 300 kb/cM in the I. scapularis genome.
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA. aff1@cdc.gov
Amy J
AJ
Ullmann
Joseph
J
Piesman
M C
MC
Dolan
William C
WC
Black
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D016678:Genome; D018884:Ixodes / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D016324:Sequence Tagged Sites
D018076:DNA, Complementary