[Inhibition of proliferation in Jurkat cells transfected with exogenous HCAP1 gene].
HCAP1 is a novel hepatic cancer related gene located on human chromosome 17p13.3. The loss of heterozygosity occurred at 17p13.3 in various human cancers. In order to investigate the effects of exogenous HCAP1 gene products on cell proliferation of T lymphoma Jurkat cell line, HCAP1 gene! was transfected into Jurkat cells mediated by liposome, and the cells stably expressing exogenous HCAP1 were screened with G418. The effects of HCAP1 products on cell proliferation were assessed by viable cell count, cell growth curve and colony formation assay in soft agar. The results showed that the HCAP1 transgenic Jurkat cells displayed slow growth rate, extended doubling time and reduced colony formation capability, as compared with the cells transfected with pBK/CMV empty vector (P < 0.01). It is concluded that exogenous HCAP1 gene products could inhibit the proliferation of Jurkat cells.
Wu
Xiang-Hua
XH
Institute of Hematology, Jinan University Medical College, Guangzhou 510632, China.
Wang
Rong
R
Du
Jun-Xiang
JX
Mu
Qi-Tian
QT
Guo
Lie-Ping
LP
Zhen
Pei-Er
PE
Wan
Da-Fang
DF
Gu
Jian-Ren
JR
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002455:Cell Division; D006801:Humans; D019169:Jurkat Cells; D008113:Liver Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D010455:Peptides; D014162:Transfection
D009363:Neoplasm Proteins; D010455:Peptides; C442243:dermcidin