Induction of CD13 on T-lymphocytes by adhesive interaction with gingival fibroblasts.
Lymphocytes in peripheral blood do not express CD13 (aminopeptidase N), a membrane alanyl metallopeptidase. However, it has been demonstrated that locally infiltrated lymphocytes in chronic inflammatory sites can be CD13-positive, and possible involvement of stromal cell adherence in the induction of CD13 has been suggested. In this study, we examined whether T-lymphocyte/gingival-fibroblast interaction can activate T-lymphocytes to express CD13. CD13 expression was induced on PMA-activated T-lymphocytes only when they adhered directly to human gingival fibroblasts (HGF) at 2 hrs after the co-culture began, while an increase in the enzyme activity of CD13 was also confirmed in activated T-lymphocytes that had been co-cultured with HGF. Furthermore, CD13-positive T-lymphocytes were detected in inflamed gingival tissues in vivo. Analysis of these results indicates that direct interaction with HGF is essential for the induction of CD13 expression on T-lymphocytes that was also observed in periodontitis lesions.
Saho
T
T
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan.
Kishida
T
T
Hirano
H
H
Hashikawa
T
T
Shimabukuro
Y
Y
Murakami
S
S
D018826:CD13 Antigens / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques; D004790:Enzyme Induction; D005347:Fibroblasts / Q000201:enzymology / Q000502:physiology; D005881:Gingiva / Q000166:cytology / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology; D006801:Humans; D008213:Lymphocyte Activation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000502:physiology
D018826:CD13 Antigens