Use of the Keele injector for sample introduction for gas chromatographic analysis of vinclozolin in lettuces.
We examined a Keele injector for sample introduction for gas chromatographic analysis of vinclozolin treated in lettuces. Samples in milligram quantity were introduced into a glass tube in a Keele injector at a gas chromatograph injection port. The glass tube was then crushed to allow the sample to carry onto a capillary column in a normal manner. The standard calibration curve for quantitative detection of vinclozolin was obtained by determining vinclozolin spiked in samples at variable concentrations. The calibration curve showed a linear response to vinclozolin ranging from 0.05 to 1.0 microg/g, giving a slope value of 174.8, the y-intercept value of -2.8146 and the mean r2-value of 0.9994. Limit of quantification for vinclozolin was 0.05 microg/g by this method, comparable to 0.01 microg/g by a normal injector. When samples treated previously with vinclozolin were determined by the Keele injector, vinclozolin was found to be about 30% lower as compared to a normal method, suggesting about 70% recovery of the spiked vinclozolin by the Keele injector. From these results, the Keele injector was suggested to be potential for sample introduction in gas chromatographic analysis of vinclozolin in lettuce samples.
Shim
J H
JH
Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, South Korea. jhshim@chonnam.ac.kr
Lee
Y S
YS
Kim
M R
MR
Lee
C J
CJ
Kim
I S
IS
D002849:Chromatography, Gas / Q000379:methods*; D018545:Lactuca / Q000737:chemistry*; D010080:Oxazoles / Q000032:analysis*; D012680:Sensitivity and Specificity
D010080:Oxazoles; C025643:vinclozolin