[Distribution functions, describing the binding of extended ligands with DNA molecules. Possible use for cases of DNA condensation].
Due to noncooperative binding of ligands to DNA molecules, DNA molecules are in equilibrium with different numbers of adsorbed ligands. This equilibrium for a given concentration of the free ligand in the solution is characterized by the distribution function, which describes the probability of revealing the DNA molecule with a definite number of adsorbed ligands. If polycations act as ligands, DNA molecules with the number of ligands sufficient for neutralizing the charges on phosphates may undergo a phase transition. One example of this transition is the formation of liquid-crystalline dispersions during the binding of DNA to chitosan. We analyzed the binding of chitosan to DNA on the assumption that this binding is due to equilibrium adsorption. At a definite concentration of chitosan in solution, DNA molecules are in equilibrium with different numbers of adsorbed molecules of chitosan. If the number of adsorbed ligands exceeds some critical value, the DNA molecule covered with chitosan becomes capable of interacting with other DNA molecules. As a result of this interaction (attraction), liquid-crystalline dispersions can form. Equations describing the dependence of the concentration of DNA molecules on the concentration of the ligand in solution were derived. It was shown that, at given parameters of the model, it is possible to describe experimental data characterizing the formation of cholesteric liquid-crystalline dispersions. The analysis of the data makes it possible to reconstitute both the size of the binding site occupied by chitosan on the DNA and the energy of interaction of chitosan with DNA.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Lomonosov Moscow State University, Bioanaliticheskie Tekhnologii Company, Moscow, Russia. nech@imb.ac.ru
Iu D
IuD
Nechipurenko
A M
AM
Vol'f
Iu M
IuM
Evdokimov
D000327:Adsorption; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands
D008024:Ligands; D002686:Chitin; D004247:DNA; D048271:Chitosan