Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identification of S. enterica serovar Typhimurium.
Department of Clinical Laboratory Science, College of Health Sciences, Korea University, Seoul 136-703, Korea.
Young-Hee
YH
Lim
Kenji
K
Hirose
Hidemasa
H
Izumiya
Eiji
E
Arakawa
Hideyuki
H
Takahashi
Jun
J
Terajima
Ken-ichiro
K
Itoh
Kazumichi
K
Tamura
Sung-Il
SI
Kim
Haruo
H
Watanabe
D016133:Polymerase Chain Reaction / Q000379:methods*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*