[Celastrol in the inhibition of neovascularization].
OBJECTIVE
To study the inhibition effect of celastrol on neovascularization.


METHODS
The effect of celastrol on the in vitro proliferation of endothelial cell of vessel (ECV) was examined by MTT assay. The effect of celastrol on endothelial cell migration, tube formation on Matrigel and Chick chorioallantoic membrane angiogenesis was also examined. Matrigel plug assay was used to evaluate the effect of celastrol on angiogenesis in vivo.


RESULTS
The proliferation of ECV was inhibited significantly by celastrol with IC(50) being 1.33 microg/ml. Celastrol inhibited endothelial cell migration and tube formation in a dose-dependent manner. Celastrol also inhibited angiogenesis both in Matrigel plug of mouse model and in chick chorioallantoic membranes.


CONCLUSIONS
Celastrol, which can inhibit angiogenesis, could be developed as an antiangiogenic drug.
Huang
Yu-lun
YL
Department of Neurosurgery, First Hospital, Soochow University, Suzhou 215006, China.
Zhou
You-xin
YX
Zhou
Dai
D
Xu
Qi-nian
QN
Ye
Ming
M
Sun
Cheng-fa
CF
Du
Zi-wei
ZW
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D042783:Endothelial Cells / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D053978:Pentacyclic Triterpenes; D014315:Triterpenes / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C050414:celastrol