[Determination of glycyrrhizic acid binding sites by a phage display method].
Phages that expose peptides specifically interacting with glycyrrhizic acid (GA) were selected from a phage peptide library by affinity selection and ELISA. Amino acid sequence analysis of the selected peptides and human proteins with the SIM program revealed homology to tyrosine protein kinases, serine/threonine protein kinases, tyrosine phosphatases, and some receptors. Analysis of the peptide and virus protein sequences with the BLAST program showed that GA has affinity for various surface proteins of several human viruses such as HIV-1, hepatitis C virus, and herpesviruses.
Il'ina
T V
TV
VECTOR State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 630559 Russia. tilina@ngs.ru
Fediuk
N V
NV
Bachinskiĭ
A G
AG
Tumanova
O Iu
OIu
Kuvshinov
V N
VN
Il'ichev
A A
AA
Pokrovskiĭ
A G
AG
D019380:Anti-HIV Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D019695:Glycyrrhizic Acid / Q000378:metabolism* / Q000494:pharmacology; D015497:HIV-1 / Q000378:metabolism; D016174:Hepacivirus / Q000378:metabolism; D006564:Herpesviridae / Q000378:metabolism; D019151:Peptide Library
D019380:Anti-HIV Agents; D017931:DNA Primers; D019151:Peptide Library; D019695:Glycyrrhizic Acid