On-line concentration by field-enhanced sample injection with reverse migrating micelles in micellar electrokinetic capillary chromatography for the analysis of flavonoids in Epimedium brevicornum Maxim.
A simple and sensitive micellar electrokinetic capillary chromatography (MEKC) method was developed for the separation and determination of six flavonoids in Epimedium brevicornum Maxim. Field-enhanced sample injection with reverse migrating micelles (FESI-RMM) was used for on-line concentration of the flavonoids. An electrolyte containing 20 mM H3PO4, 100 mM SDS, 20% acetonitrile and 2% 2-propanol (pH 2.0) was chosen as the electrophoretic buffer. By optimizing the stacking conditions, about 40-360-fold improvement in the detection sensitivity was obtained for the flavonoids.
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Shufang
S
Wang
Yanqi
Y
Wu
Yong
Y
Ju
Xingguo
X
Chen
Wenjie
W
Zheng
Zhide
Z
Hu
D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D008823:Micelles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002021:Buffers; D005419:Flavonoids; D008823:Micelles