Amino acid ingestion improves muscle protein synthesis in the young and elderly.
We recently demonstrated that muscle protein synthesis was stimulated to a similar extent in young and elderly subjects during a 3-h amino acid infusion. We sought to determine if a more practical bolus oral ingestion would also produce a similar response in young (34 +/- 4 yr) and elderly (67 +/- 2 yr) individuals. Arteriovenous blood samples and muscle biopsies were obtained during a primed (2.0 micromol/kg) constant infusion (0.05 micromol.kg(-1).min(-1)) of L-[ring-2H5]phenylalanine. Muscle protein kinetics and mixed muscle fractional synthetic rate (FSR) were calculated before and after the bolus ingestion of 15 g of essential amino acids (EAA) in young (n = 6) and elderly (n = 7) subjects. After EAA ingestion, the rate of increase in femoral artery phenylalanine concentration was slower in elderly subjects but remained elevated for a longer period. EAA ingestion increased FSR in both age groups by approximately 0.04%/h (P < 0.05). However, muscle intracellular (IC) phenylalanine concentration remained significantly higher in elderly subjects at the completion of the study (young: 115.6 +/- 5.4 nmol/ml; elderly: 150.2 +/- 19.4 nmol/ml). Correction for the free phenylalanine retained in the muscle IC pool resulted in similar net phenylalanine uptake values in the young and elderly. EAA ingestion increased plasma insulin levels in young (6.1 +/- 1.2 to 21.3 +/- 3.1 microIU/ml) but not in elderly subjects (3.0 +/- 0.6 to 4.3 +/- 0.4 microIU/ml). Despite differences in the time course of plasma phenylalanine kinetics and a greater residual IC phenylalanine concentration, amino acid supplementation acutely stimulated muscle protein synthesis in both young and elderly individuals.
Paddon-Jones
Douglas
D
Department of Surgery, The University of Texas Medical Branch, Galveston, Texas 77550, USA. djpaddon@utmb.edu
Sheffield-Moore
Melinda
M
Zhang
Xiao-Jun
XJ
Volpi
Elena
E
Wolf
Steven E
SE
Aarsland
Asle
A
Ferrando
Arny A
AA
Wolfe
Robert R
RR
D000284:Administration, Oral; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000502:physiology*; D000601:Amino Acids, Essential / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D007444:Inulin / Q000097:blood*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D010649:Phenylalanine / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism
D000601:Amino Acids, Essential; D009124:Muscle Proteins; D010649:Phenylalanine; D007444:Inulin