Enzymes activity of intestinal grafts after liver small bowel transplantation in rats.
OBJECTIVE
To investigate the activity alterations of enzymes in intestine grafts after liver/small bowel transplantation in rats and the relations of these changes to immune rejection of grafts.


METHODS
A model of liver/small bowel transplantation (LSBT) was established in closed colony SD and Wistar rats. The activity of enzymes including triphosphatase (ATPase), alkalinophosphatase (AKP), acytelcholinesterase (AchE), oxidesynthase (NOS) and monoamine oxidase (MAO) in bowel grafts was studied histochemically at regular postoperative intervals.


RESULTS
The activity of enzymes in the wall of the grafts disappeared eventually in isolated small bowel transplantation (SBT) rats. In contrast, the activity in LSBT rats remained and recovered postoperatively.


CONCLUSIONS
The rejection in grafted intestine could be prevented or delayed in LSBT rats. The changes in the activity of enzymes and neurons might be used to detect the rejection and function of the graft.
Sun
Xiao-Yi
XY
Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xysuna85@public.wh.hb.cn
Wang
Guo
G
Shi
Hui-Fen
HF
Wei
Ming-Fa
MF
Yin
Guang-Fu
GF
Ru
Li-Qiang
LQ
D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D006084:Graft Rejection / Q000473:pathology; D006085:Graft Survival / Q000502:physiology*; D007421:Intestine, Small / Q000201:enzymology* / Q000294:innervation / Q000637:transplantation*; D008099:Liver / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology; D008995:Monoamine Oxidase / Q000378:metabolism; D009412:Nerve Fibers / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D008995:Monoamine Oxidase; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases