Involvement of poly(ADP-ribose) synthetase in acoustic trauma of the cochlea.
We investigated effects of poly(ADP-ribose) synthetase (PARS) inhibitors on acoustic trauma. Albino guinea pigs were intravenously given 3-aminobenzamide, nicotinamide or 3-aminobenzoic acid (an inactive analog of 3-aminobenzamide) just prior to exposure to a 2 kHz pure tone of 120 dB sound pressure level (SPL) for 10 minutes. The threshold of the compound action potential (CAP) and the amplitude of distortion-product otoacoustic emissions (DPOAEs) were measured before and 4 hours after the acoustic overexposure. Statistically significant decreases in the CAP threshold shifts and significant increases in the DPOAE amplitudes were observed 4 hours after the acoustic overexposure in the animals treated with 3-aminobenzamide or nicotinamide, whereas 3-aminobenzoic acid did not exert any protective effect. These results strongly suggest that excessive activation of PARS is involved in generation of the acoustic trauma.
Department of Otolaryngology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba 305-8575, Japan. ktabuchi@md.tsukuba.ac.jp
Keiji
K
Tabuchi
Tomohumi
T
Hoshino
Hidekazu
H
Murashita
Keiko
K
Oikawa
Isao
I
Uemaetomari
Bungo
B
Nishimura
Tadamichi
T
Tobita
Akira
A
Hara
D000161:Acoustic Stimulation / Q000009:adverse effects; D000200:Action Potentials / Q000502:physiology; D062365:Aminobenzoates / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D003051:Cochlea / Q000473:pathology*; D004789:Enzyme Activation; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000378:metabolism; D009536:Niacinamide / Q000008:administration & dosage / Q000378:metabolism; D017084:Otoacoustic Emissions, Spontaneous; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D062368:meta-Aminobenzoates
D062365:Aminobenzoates; D001549:Benzamides; D018696:Neuroprotective Agents; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D062368:meta-Aminobenzoates; D009536:Niacinamide; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; C044855:3-aminobenzoic acid