7, 8-dihydroxyflavanone as an inhibitor for Jun-Fos-DNA complex formation and its cytotoxic effect on cultured human cancer cells.
7,8-Dihydroxyflavanone, isolated from the seeds of Alpinia Katsumadai Hayata, showed an inhibitory effect on Jun-Fos dimer action. 7,8-Dihydroxyflavanone blocked the action of the dimer on a DNA consensus sequence, the AP-1 binding site. We have concluded that the Jun-Fos heterodimer, bound with 7,8-dihydroxyflavanone, cannot bind to the AP-1 site and therefore results in signal interruption. The 7,8-dihydroxyflavanone was also found to have an in vitro cytotoxic effect against A549 (a human lung cancer cell line) and K562 (a human leukemia cell line).
Hahm
Eun-Ryeong
ER
School of Chemistry and Molecular Engineering, College of Natural Sciences, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul, 151-742, Korea.
Park
Seyeon
S
Yang
Chul-Hak
CH
D032429:Alpinia / Q000737:chemistry*; D018736:DNA Adducts; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D016762:Genes, fos / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics*; D007938:Leukemia / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
C478869:7,8-dihydroxyflavanone; D018736:DNA Adducts; D044950:Flavanones; D018808:Transcription Factor AP-1