Kainic acid upregulates uncoupling protein-2 mRNA expression in the mouse brain.
Intraperitoneal injection of kainic acid (KA) in C57BL/6J and 129T2SvEmsJ mice led to a transient induction of uncoupling protein-2 (Ucp2) mRNA expression in several brain regions, which included the CA1 subfield of the hippocampus, the dorsal endopiriform nucleus and the piriform cortex in both strains. In all those regions, levels of Ucp2 mRNA expression, as determined by in situ hybridization, peaked at 24 h and returned to basal levels within 72 h post-injection. The increase in mRNA expression was mainly observed in neurons, with microglial cells displaying only scattered expression of the gene. The neuronal induction of Ucp2 in response to KA was stronger in 129T2SvEmsJ mice than in C57BL/6J, which suggests a role for Ucp2 in excitotoxic challenges and neuroprotection.
Centre de recherche de l'Hôpital Laval, Institut universitaire de cardiologie et de pneumologie, Université Laval, Québec GIK7P4, Canada.
Sébastien
S
Clavel
Eric
E
Paradis
Daniel
D
Ricquier
Denis
D
Richard
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000071246:Uncoupling Protein 2; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009498:Neurotoxins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D007608:Kainic Acid