Molecular characterization of major histocompatibility complex class 1 (MHC-I) from squirrel monkeys (Saimiri sciureus).
Little is known about the major histocompatibility complex (MHC) class 1 in squirrel monkeys ( Saimiri sciureus). We cloned, sequenced and characterized two alleles and the cDNA of the coding region of MHC class 1 in these New World monkeys. Phylogenetic analyses showed that these sequences are related to HLA class 1 genes ( HLA-A and HLA-G). The structure and organization of one of the two identified clones was similar to that of a class 1 MHC gene ( HLA-A2). All the exon/intron splice acceptor/donor sites are conserved and their locations correspond to the HLA-A2 gene. The sequences of the newly described cDNAs reveal that they code for the characteristic class 1 MHC proteins, with all the features thought necessary for cell surface expression. Typical sequences for the leader peptide, alpha(1), alpha(2), alpha(3), transmembrane and cytoplasmic domains were found.
Pascalis
Hervé
H
Laboratoire de Rétrovirologie, Institut Pasteur de la Guyane, 23 avenue Pasteur, BP 6010, 97306 Cayenne, French Guiana.
Heraud
Jean-Michel
JM
Fendel
Rolf
R
Lavergne
Anne
A
Kazanji
Mirdad
M
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005091:Exons; D005805:Genes, MHC Class I / Q000235:genetics*; D006680:HLA Antigens / Q000737:chemistry / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000737:chemistry / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D012453:Saimiri / Q000235:genetics* / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I