PEG-inhibitor conjugates: application of diphenyl alpha-aminoalkylphosphonate to removal of chymotrypsin.
Diphenyl 1-amino-2-phenylethylphosphonate was introduced to poly(ethylene glycol)s (PEGs) with average molecular masses of 300, 400, and 600 to prepare water-insoluble PEG-inhibitor conjugates. Interestingly, only the conjugate from PEG with an average molecular weight of 600 formed a precipitate with chymotrypsin but not with trypsin. The results demonstrated that the PEG-inhibitor conjugate is useful for separation of chymotrypsin.
Umezaki
Makiko
M
Department of Material Systems Engineering and Life Science, Faculty of Engineering, Toyama University, Gofuku, Toyama, Japan.
Fujii
Takayoshi
T
Yoshimura
Toshiaki
T
Yamazaki
Isao
I
Ono
Shin
S
D011232:Chemical Precipitation; D002918:Chymotrypsin / Q000302:isolation & purification*; D008722:Methods; D008970:Molecular Weight; D063065:Organophosphonates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000302:isolation & purification; D014357:Trypsin
D063065:Organophosphonates; D011480:Protease Inhibitors; D011092:Polyethylene Glycols; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin