In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor.
The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.
Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. pekka.rappu@utu.fi
Pekka
P
Rappu
Terhi
T
Pullinen
Pekka
P
Mäntsälä
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D010754:Phosphoribosyl Pyrophosphate / Q000737:chemistry / Q000378:metabolism*; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; C065825:PurR protein, Bacteria; D012097:Repressor Proteins; D010754:Phosphoribosyl Pyrophosphate