LEA (late embryonic abundant)-like protein Hsp 12 (heat-shock protein 12) is present in the cell wall and enhances the barotolerance of the yeast Saccharomyces cerevisiae.
Yeast cells Saccharomyces cerevisiae, late embryogenic abundant-like stress response protein Hsp 12 (heat-shock protein 12) were found by immunocytochemistry to be located both in the cytoplasm and in the cell wall, from where they could be extracted with dilute NaOH solutions. Yeast cells with the Hsp 12 gene disrupted were unable to grow in the presence of either 12 mM caffeine or 0.43 mM Congo Red, molecules known to affect cell-wall integrity. The volume of yeast cells were less affected by rapid changes in the osmolality of the growth medium when compared with the wild-type yeast cells, suggesting a role for Hsp 12 in the flexibility of the cell wall. This was also suggested by subjecting the yeast cells to rapid changes in barometric pressure where it was found that wild-type yeast cells were more resistant to cellular breakage.
Department of Molecular and Cellular Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Precious
P
Motshwene
Robert
R
Karreman
Gail
G
Kgari
Wolf
W
Brandt
George
G
Lindsey
D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins