Correlation between T-cell and mast cell activity in patients with chronic urticaria.
BACKGROUND
The presence of autoantibodies reacting with the high affinity IgE receptor (FcepsilonRIalpha) usually indicates a more severe form of chronic urticaria (CU). Previously, we showed an increased lymphocyte reactivity in CU patients; however, the relation between enhanced cellular immunity and the presence of anti-FcepsilonRIalpha-specific autoantibodies has not been investigated.


METHODS
Cellular and humoral immune reactivity of 50 CU patients and 28 healthy controls was studied. Serum sIL-2R, neopterin, and tryptase levels were measured to assess T-cell, monocyte/macrophage and mast cell activity, respectively. Helicobacter pylori (HP)-specific IgG antibody, and IgE levels were also tested. Anti-FcepsilonRIalpha-specific autoantibody was determined by Western blotting. In vivo histamine-releasing activity of patients' sera was assessed by the autologous serum skin test (AST).


RESULTS
17/50 CU patients, who both had IgG-type anti-FcepsilonRIalpha-antibodies by Western blotting and a positive AST response, were classified as autoimmune CU. All patients with CU had significantly higher serum sIL-2R and tryptase levels than healthy controls (p = 0.000257, p = 0.000166, respectively), indicating T-cell and mast cell activation. Patients with higher sIL-2R levels also had higher tryptase levels; the strongest correlation was shown in the autoimmune subgroup of patients (rho = 0.688, p = 0.002). There was a tendency towards higher tryptase levels in the autoimmune subgroup, as compared to the nonautoimmune CU patients. While the serum IgE was significantly lower in autoimmune than in nonautoimmune CU (p = 0.000836), there was no significant difference in their sIL-2R, neopterin and HP-specific IgG antibody levels. CU patients with a positive AST response (38/50) had significantly higher tryptase levels (p = 0.0107) when compared to the negative skin test group.


CONCLUSIONS
The significant correlation between sIL-2R and tryptase levels in patients with CU indicates that T cell activation is proportional to mast cell degranulation in these patients. The increased level of tryptase in autoimmune CU may suggest more severe disease.
Central Laboratory of Immunology, Semmelweis University, Budapest, Hungary. hiber@immun.sote.hu
Bernadett
B
Hidvégi
Eszter
E
Nagy
Teréz
T
Szabó
Erzsébet
E
Temesvári
Márta
M
Marschalkó
Sarolta
S
Kárpáti
Attila
A
Horváth
Péter
P
Gergely
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D001323:Autoantibodies / Q000276:immunology; D015153:Blotting, Western; D005260:Female; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D019798:Neopterin / Q000097:blood; D017455:Receptors, IgE / Q000276:immunology / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000097:blood; D012697:Serine Endopeptidases / Q000097:blood; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D053802:Tryptases; D014581:Urticaria / Q000276:immunology* / Q000378:metabolism
D000907:Antibodies, Bacterial; D001323:Autoantibodies; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D019798:Neopterin; D012697:Serine Endopeptidases; D053802:Tryptases