Evaluation of hydroxyl radical-scavenging abilities of silica hydride, an antioxidant compound, by a Fe2+-EDTA-induced 2-hydroxyterephthalate fluorometric analysis.
The hydroxyl radical scavenging capacity and efficacy of a novel organosiliceous anionic hydride compound, silica hydride, were quantified by a recently developed method. The method measures a direct relationship between the hydroxyl radical scavenging capability of the antioxidant compound and the linear decrease in signal from a fluorescent 2-hydroxyterephthalate product created by reacting an Fe(2+)-EDTA complex in the presence of a potential radical scavenger. A fluorescence signal half-inhibition, IC(50), value of 1.4 +/- 0.1 muM was obtained for silica hydride compounds. The validity of the analysis was verified by electron spin resonance spectroscopy, spectrophotometric analysis of NAD(+)/NADH ratios, mitochondrial membrane potential measurements, and assays of both cytochrome c (Fe(3+)) to cytochrome c (Fe(2+)) and epinephrine to adenochrome reductions.
Stephanson
Cory J
CJ
Flantech Group, Watsonville, California 95076, USA.
Stephanson
Anne M
AM
Flanagan
G Patrick
GP
D000975:Antioxidants / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000737:chemistry; D005470:Fluorometry / Q000379:methods*; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D017640:Silicates / Q000737:chemistry*; D013053:Spectrophotometry
D000975:Antioxidants; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D017640:Silicates; D017665:Hydroxyl Radical; D004492:Edetic Acid; C019179:Fe(III)-EDTA