Augmented PLA2 activity in pre-eclamptic decidual tissue--a key player in the pathophysiology of 'acute atherosis' in pre-eclampsia?
Decidual acute atherosis is associated with pre-eclampsia, but the underlying mechanism is still unclear. We have previously demonstrated elevated level of the oxidative stress marker 8-isoprostaglandin F(2 alpha)(8-isoprostane) and lipids in pre-eclamptic decidual tissue. Arachidonic acid (AA) in tissue phospholipids is a source for 8-isoprostane generation, and 8-isoprostane is liberated from tissue phospholipids by phospholipase A(2)(PLA(2)). The aims of this study were to explore whether AA content or PLA(2)expression in pre-eclamptic decidual tissue differed from controls. Decidua basalis tissues were obtained by vacuum aspiration at Caesarean delivery in pre-eclamptic and control pregnancies. We demonstrated a statistically significantly higher total PLA(2)activity in pre-eclamptic decidua compared to control tissue. On the other hand, no differences in AA content of tissue phospholipids or protein expression of secretory and cytosolic PLA(2)between pre-eclamptic and control decidual tissue were found. In conclusion, the elevated level of free 8-isoprostane in pre-eclamptic decidual tissue could be caused by augmented PLA(2)activity. We speculate that an elevated PLA(2)enzyme activity in pre-eclamptic decidual tissue could be of importance in the pathogenesis of 'acute atherosis', comparable to the atherogenesis in cardiovascular diseases.
Staff
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Department of Obstetrics and Gynecology, Ulleval University Hospital, Kirkeveien 166, 0450 Oslo, Norway. annetine.staff@uleval.no
Ranheim
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T
Halvorsen
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B
D001055:Apolipoproteins B / Q000032:analysis; D016718:Arachidonic Acid / Q000032:analysis; D001161:Arteriosclerosis / Q000209:etiology; D015992:Body Mass Index; D003600:Cytosol / Q000737:chemistry; D033301:Deciduoma / Q000737:chemistry / Q000201:enzymology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011225:Pre-Eclampsia / Q000150:complications / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D001055:Apolipoproteins B; D005227:Fatty Acids; D012333:RNA, Messenger; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2