Bioaccumulation and toxicity of sediment associated herbicides (ioxynil, pendimethalin, and bentazone) in Lumbriculus variegatus (Oligochaeta) and Chironomus riparius (Insecta).
The benthic macroinvertebrates Lumbriculus variegatus and Chironomus riparius were used in toxicity and bioaccumulation tests to determine the toxic concentrations and accumulation potential of sediment associated herbicides. The tested chemicals were ioxynil, bentazone, and pendimethalin. The bioaccumulation tests with L. variegatus were performed in four different sediments, each having different characteristics. Water-only LC(50) tests were performed with both L. variegatus and C. riparius. A sublethal effect of model compounds in sediments was assessed by a C. riparius larvae growth-inhibition test. Of the model compounds, ioxynil appeared to be the most toxic, with LC(50) values 1.79 and 2.79 mgL(-1) for L. variegatus and C. riparius, respectively. The LC(50) water concentrations for bentazone were 79.11 and 62.31 mgL(-1) for L. variegatus and C. riparius, respectively. Similarly, ioxynil revealed the highest bioaccumulation potential in bioaccumulation tests. The most important characters affecting chemical fate in the sediment seemed to be the organic matter content and the particle size fraction. The sediments with low organic material and coarse particle size consistently showed high bioaccumulation potential and vice versa. In C. riparius growth tests bentazone had a statistically significant effect on larval growth at sediment concentrations of 1160 and 4650 mgkg(-1) (P<0.05). It is noteworthy that standard deviations tend to be greater at high chemical concentrations, which addresses the fact that part of the individuals started to suffer. Ioxynil had an effect on the larval growth in other test sediment at the highest concentration (15.46 mgkg(-1)dw), in which head capsule length correlated with larval weight, decreasing toward higher exposure concentrations. The current results show the importance of sediment organic matter as a binding site of xenobiotics.
Department of Biology, University of Joensuu, P.O. Box 111, 80101, Joensuu, Finland. kimmo.maenpaa@joensuu.fi
Kimmo A
KA
Mäenpää
Arto J
AJ
Sormunen
Jussi V K
JV
Kukkonen
D000814:Aniline Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D000818:Animals; D001581:Benzothiadiazines / Q000493:pharmacokinetics* / Q000633:toxicity*; D002683:Chironomidae; D019015:Geologic Sediments / Q000737:chemistry; D006540:Herbicides / Q000493:pharmacokinetics* / Q000633:toxicity*; D007462:Iodobenzenes / Q000493:pharmacokinetics* / Q000633:toxicity*; D007814:Larva / Q000254:growth & development; D007928:Lethal Dose 50; D009570:Nitriles / Q000493:pharmacokinetics* / Q000633:toxicity*; D009835:Oligochaeta; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity*
D000814:Aniline Compounds; D001581:Benzothiadiazines; D006540:Herbicides; D007462:Iodobenzenes; D009570:Nitriles; D014874:Water Pollutants, Chemical; C008161:ioxynil; C002003:bentazone; C030856:pendimethalin