Sequence analysis of cyclodextrin glycosyltransferase from the alkaliphilic Bacillus agaradhaerens.
The gene encoding an alkaline active cyclodextrin glycosyltransferase (CGTase) from the alkaliphilic B. agaradhaerens LS-3C was cloned and sequenced. It encodes a mature polypeptide of 679 amino acids with a molecular mass of 76,488 Da. The deduced amino acid sequence of the mature CGTase revealed 99 and 95% identity to the CGTase sequences from the other B. agaradhaerens strains, DSM 8721T and 9948, respectively. The next closest identity was of 59% with B. clarkii enzyme. CGTases from B. agaradhaerens, B. clarkii, and B. firmus/lentus formed a phylogenetically separated cluster from the other CGTases of Bacillus spp. origin. A number of usually conserved residues in the CGTases were found to be replaced in the sequence of B. agaradhaerens enzyme. The sequence analysis indicated the enzyme to be close to the so-called 'intermediary enzymes' in the alpha-amylase family.
Martins
Rita F
RF
Department of Biotechnology, Center for Chemistry & Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Delgado
Osvaldo
O
Hatti-Kaul
Rajni
R
D000595:Amino Acid Sequence; D001407:Bacillus / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008967:Molecular Biology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D011994:Recombinant Proteins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase