Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine.
The spatial structure of the Ca(2+)-regulated photoprotein obelin has been solved to resolution of 1.1A. Two oxygen atoms are revealed substituted at the C2-position of the coelenterazine in contrast to the obelin structure at 1.73A resolution where one oxygen atom only was disclosed. The electron density of the second oxygen atom was very weak but after exposing the crystals to a trace of Ca(2+), the electron densities of both oxygen atoms became equally intense. In addition, one Ca(2+) was found bound in the loop of the first EF-hand motif. Four of the ligands were provided by protein residues Asp30, Asn32, Asn34, and the main chain oxygen of Lys36. The other two were from water molecules. From a comparison of B-factors for the residues constituting the active site, it is suggested that the variable electron densities observed in various photoprotein structures could be attributed to different mobilities of the peroxy oxygen atoms.
Liu
Zhi-Jie
ZJ
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
Vysotski
Eugene S
ES
Deng
Lu
L
Lee
John
J
Rose
John
J
Wang
Bi-Cheng
BC
D001665:Binding Sites; D002118:Calcium / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D003461:Crystallography / Q000379:methods*; D004583:Electrons; D007093:Imidazoles; D008164:Luminescent Proteins / Q000737:chemistry*; D046911:Macromolecular Substances; D036103:Nanotechnology / Q000379:methods; D010100:Oxygen / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D021122:Protein Subunits; D011719:Pyrazines / Q000737:chemistry*; D012996:Solutions
D007093:Imidazoles; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D021122:Protein Subunits; D011719:Pyrazines; D012996:Solutions; C010497:obelin; C017144:coelenterazine; D002244:Carbon; D010100:Oxygen; D002118:Calcium