Cytochrome P450 2J2 polymorphism in healthy Caucasians and those with diabetes mellitus.
OBJECTIVE
Cytochrome P450 (CYP) 2J2 plays an important role in the biosynthesis of the biologically active cis-epoxyeicosatrienoic acids. An allelic variant named CYP2J2*6, which encodes an enzyme that is almost inactive in the metabolism of arachidonic acid, has recently been described. We investigated the frequency of the CYP2J2*6 variant in a Caucasian population and the relationship between this polymorphism and the development of micro- and macrovascular complications and hypertension in patients with type 1 or type 2 diabetes mellitus.


METHODS
Genomic DNA was extracted from peripheral blood cells and the fragment containing the A/T single nucleotide polymorphism at position 25 661 in exon 8 of the CYP2J2 gene was amplified. The 532 bp amplified product was subsequently digested with Tsp509I and analyzed on 12% polyacrylamide gel electrophoresis.


RESULTS
In the whole population, the frequency of the CYP2J2*6 allele was 0.0064 and the frequency of the CYP2J2*1 allele was 0.9936. Genotype distribution did not show significant differences between controls and patients with type 1 or type 2 diabetes. No homozygotes for CYP2J2*6 allele were found. No association was found between this allele and complications or hypertension in either type of diabetes.


CONCLUSIONS
The CYP2J2*6 allele is rare in the Caucasian population, and no association is inferred between this allelic variant and diabetic complications.
Department of Endocrinology and Metabolism, Unit of Diabetes and Metabolism, Faculty of Medicine, University of Pisa, Pisa, Italy.
Laura
L
Pucci
Daniela
D
Lucchesi
Vera
V
Chirulli
Giuseppe
G
Penno
Inger
I
Johansson
Pier
P
Gervasi
Stefano
S
Del Prato
Vincenzo
V
Longo
D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D006580:Genetic Carrier Screening; D014644:Genetic Variation; D006801:Humans; D010105:Oxygenases / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D044465:White People / Q000235:genetics*
C000717350:CYP2J2 protein, human; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2