[Activity of 5-aminolevulinate synthase in rat liver during degradation of cytochrome P-450 caused by administration of cadmium chloride].
The 5-aminolevulinate synthase, tryptophan-2,3-dioxygenase activities and cytochrome P-450 content in the rat liver was studied in different terms after CdCl2 administration and after administration of metal salt against a background of 2-hours action of alpha-tocopherol. The lowering of activity of 5-aminolevulinate synthase in 2 h with the consequent increase of the enzyme activity in 6 h and 24 h was detected. The holoenzyme activity and heme saturation of tryptophan-2,3-dioxygenase increased 6 h after CdCl2 administration. The holoenzyme activity and the total activity of tryptophan-2,3-dioxygenase rised in 24 h. The level of cytochrome P-450 lowered. Preliminary administration of alpha-tocopherol prevented changes of studied parameters 24 h after CdCl2 administration. The relationship between decrease of cytochrome P-450 level and 5-aminolevulinate synthase activation are discussed.
Karazin Kharkov National University, Ukraine, Kharkiv. Pavel.A.Kaliman@univer.kharkov.ua
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Kaliman
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Inshina
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism*; D000818:Animals; D019256:Cadmium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004789:Enzyme Activation; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014366:Tryptophan Oxygenase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
D014810:Vitamin E; D003577:Cytochrome P-450 Enzyme System; D014366:Tryptophan Oxygenase; D000624:5-Aminolevulinate Synthetase; D019256:Cadmium Chloride