Ion trap tandem mass spectrometric determination of F2-isoprostanes.
A new tandem mass spectrometry (MS/MS) method based on the use of an ion trap mass spectrometer for the identification and quantitation of F(2)-isoprostanes has been developed. It consists of two solid-phase extractions and two derivation steps followed by gas chromatography/negative ion chemical ionization tandem mass spectrometry (GC/NICI-MS/MS) analysis. This method is highly selective and sensitive and it has been successfully applied to biological samples.
Signorini
Cinzia
C
Department of Pathophysiology, Experimental Medicine and Public Health, University of Siena, Via Aldo Moro, 53100 Siena, Italy.
Comporti
Mario
M
Giorgi
Gianluca
G
D000818:Animals; D002138:Calibration; D028441:F2-Isoprostanes / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D051381:Rats; D012680:Sensitivity and Specificity
D028441:F2-Isoprostanes; D007477:Ions