SNARE-protein-mediated disease resistance at the plant cell wall.
Failure of pathogenic fungi to breach the plant cell wall constitutes a major component of immunity of non-host plant species--species outside the pathogen host range--and accounts for a proportion of aborted infection attempts on 'susceptible' host plants (basal resistance). Neither form of penetration resistance is understood at the molecular level. We developed a screen for penetration (pen) mutants of Arabidopsis, which are disabled in non-host penetration resistance against barley powdery mildew, Blumeria graminis f. sp. hordei, and we isolated the PEN1 gene. We also isolated barley ROR2 (ref. 2), which is required for basal penetration resistance against B. g. hordei. The genes encode functionally homologous syntaxins, demonstrating a mechanistic link between non-host resistance and basal penetration resistance in monocotyledons and dicotyledons. We show that resistance in barley requires a SNAP-25 (synaptosome-associated protein, molecular mass 25 kDa) homologue capable of forming a binary SNAP receptor (SNARE) complex with ROR2. Genetic control of vesicle behaviour at penetration sites, and plasma membrane location of PEN1/ROR2, is consistent with a proposed involvement of SNARE-complex-mediated exocytosis and/or homotypic vesicle fusion events in resistance. Functions associated with SNARE-dependent penetration resistance are dispensable for immunity mediated by race-specific resistance (R) genes, highlighting fundamental differences between these two resistance forms.
Collins
Nicholas C
NC
Sainsbury Laboratory John Innes Centre, Norwich, Norfolk NR4 7UH, UK.
Thordal-Christensen
Hans
H
Lipka
Volker
V
Bau
Stephan
S
Kombrink
Erich
E
Qiu
Jin-Long
JL
Hückelhoven
Ralph
R
Stein
Mónica
M
Freialdenhoven
Andreas
A
Somerville
Shauna C
SC
Schulze-Lefert
Paul
P
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology* / Q000382:microbiology; D003001:Cloning, Molecular; D005658:Fungi / Q000276:immunology*; D001467:Hordeum / Q000166:cytology / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D011485:Protein Binding; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins
D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C479425:PEN1 protein, Arabidopsis; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins