[FasL-cDNA transfected into mouse bone marrow cells ex vivo to prevent graft versus host disease].
To explore the new approach to prevent graft versus host disease (GVHD) by purging ex vivo T lymphocytes of bone marrow graft through Fas-FasL way, FasL-cDNA was transfected into BALB/c mouse bon e marrow cells by liposome ex vivo. The transfected cells were cultured together with BAC (BALB/c x C57BL/6) mouse bone marrow graft. The mixing bone marrow graft was infused into BALB/c mouse recipients after 60Co-gamma irradiation. The mortality, manifestation and pathologic change of GVHD in recipient mice were observed. The CFU-S and Y chromosome from donor mice were detected. The results showed that compared with control group, the mortality in 60 days of the recipients in the experimental group decreased (20% vs 70%, P < 0.01) and the morbidity of GVHD lowered (40% vs 100%, P < 0.01). The CFU-S counts for all groups were at normal level on 20 days after transplantation. The Y chromosome from donor mice was discovered in 70% bone marrow nucleated cells of recipient mice survived over 2 months in the experimental group. It is concluded that mFasL-cDNA transfected mouse bone marrow cells prevent GVHD after culturing together with bone marrow graft, and accelerate hematopoietic reconstitution in recipient mice.
Xu
Zhi-Liang
ZL
Department of Pediatrics, People's Hospital, Wuhan University, Wuhan 430060, China.
Zou
Ping
P
Liu
Ling-Bo
LB
Li
Ai-Xiang
AX
Ma
Yan-Ping
YP
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D053222:Fas Ligand Protein; D005260:Female; D015316:Genetic Therapy; D006086:Graft vs Host Disease; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins