Inhibition of influenza A virus sialidase activity by sulfatide.
Sulfatide, which binds to influenza A viruses and prevents the viral infection, was found to inhibit the sialidase activities of influenza A viruses in a pH-dependent manner. The kinetic parameters of the effect of sulfatide on the sialidase activities of human influenza A viruses using fluorometric assay indicated that sulfatide was a powerful and non-competitive type inhibitor in low-pH conditions.
Suzuki
Takashi
T
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 422-8526 Shizuoka, Japan. suzukit@ys7.u-shizuoka-ken.ac.jp
Takahashi
Tadanobu
T
Nishinaka
Daisuke
D
Murakami
Masanori
M
Fujii
Satoshi
S
Hidari
Kazuya I-P Jwa
KI
Miyamoto
Daisei
D
Li
Yu Teh
YT
Suzuki
Yasuo
Y
D000818:Animals; D001717:Birds; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D005470:Fluorometry; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D009980:Influenza A virus / Q000201:enzymology*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D013433:Sulfoglycosphingolipids / Q000378:metabolism / Q000494:pharmacology*; D013552:Swine
D004791:Enzyme Inhibitors; D008081:Liposomes; D013433:Sulfoglycosphingolipids; C036137:2-deoxy-2,3-dehydro-N-acetylneuraminic acid; D006923:Hymecromone; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid