Metabolism-enhanced tumor localization by fluorescence imaging: in vivo animal studies.
We present a high-sensitivity near-infrared optical imaging system for noninvasive cancer detection and localization based on molecularly labeled fluorescent contrast agents. This frequency-domain system utilizes the interferencelike pattern of diffuse photon density waves to achieve high detection sensitivity and localization accuracy for the fluorescent heterogeneity embedded inside the scattering media. A two-dimensional localization map is obtained through reflectance probe geometry and goniometric reconstruction. In vivo measurements with a tumor-bearing mouse model by use of the novel Cypate-mono-2-deoxy-glucose fluorescent contrast agent, which targets the enhanced tumor glycolysis, demonstrate the feasibility of detection of a 2-cm-deep subsurface tumor in the tissuelike medium, with a localization accuracy within 2-3 mm.
Chen
Y
Y
Department of Biophysics and Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. chenyu@mail.med.upenn.edu
Zheng
G
G
Zhang
Z H
ZH
Blessington
D
D
Zhang
M
M
Li
H
H
Liu
Q
Q
Zhou
L
L
Intes
X
X
Achilefu
S
S
Chance
B
B
D000818:Animals; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D005240:Feasibility Studies; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006019:Glycolysis; D018197:Hepatoblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D017785:Photons; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared
D005456:Fluorescent Dyes; D003847:Deoxyglucose