Oxidative DNA damage (8-hydroxydeoxyguanosine) and body iron status: a study on 2507 healthy people.
To clarify the relationship of oxidative stress and body iron status, we detected urinary 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative DNA damage, and measured serum ferritin and total iron-binding capacity (TIBC), both reflecting body iron store, on 2507 healthy people aged between 22 and 89 years (males, 1253; females, 1254). The urinary 8-OHdG excretion of males showed almost no change with age, but the excretion of premenopausal females was lower than that of males, whereas postmenopausal females excreted significantly more than males. The values of serum ferritin showed no remarkable change with age in males, but increased gradually in postmenopausal females without iron loss due to bleeding, although the males' values remained higher than those of females at all ages (p<.05). On the other hand, the values of TIBC remained within the narrow limits in males, regardless of age, whereas those of females always stayed at a higher level than the males (p<.05). Conclusively, urinary 8-OHdG correlated with serum ferritin positively and with TIBC inversely, which suggested that body iron status would control the generation of 8-OHdG in vivo. After all, the increase of urinary 8-OHdG excretion in postmenopausal females may be caused by the decrease of body iron loss.
Medicine and Health Sciences Institute, Tokyo Medical University, Tokyo, Japan. m-naka50@ah2.mopera.ne.jp
Masaru
M
Nakano
Yoshikazu
Y
Kawanishi
Seika
S
Kamohara
Yoshiko
Y
Uchida
Mami
M
Shiota
Yuka
Y
Inatomi
Toyonori
T
Komori
Katsumi
K
Miyazawa
Kazumi
K
Gondo
Ikuhiro
I
Yamasawa
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000652:urine; D001823:Body Composition; D002138:Calibration; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine*; D005260:Female; D005293:Ferritins / Q000097:blood; D006801:Humans; D007501:Iron / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012727:Sex Characteristics
D000080242:8-Hydroxy-2'-Deoxyguanosine; D005293:Ferritins; D007501:Iron; D003849:Deoxyguanosine