Increased expression of the Huntingtin interacting protein-1 gene in cells from Hutchinson Gilford Syndrome (Progeria) patients and aged donors.
Hutchinson Gilford syndrome (progeria [PG]) is a human disease associated with accelerated aging. To elucidate the acceleration mechanism, we first tried to transform a PG-derived cell line by infection of a recombinant adenovirus expressing HPV (human papilloma virus)-E6 and HPV-E7 genes. The transfected PG cells had a greater number of population doublings (PD) (>80), faster doubling time, and less staining of senescence-associated ss-galactosidase than the nontransfected PG cells. The transfected cells also showed markedly more detectable telomerase activity than the nontransformed cells. The expression levels of the genes in the E6-transduced and E7-transduced cell line were then compared with those of the nontransfected cell line using an mRNA differential display method, following reverse-transcriptase polymerase chain reaction analysis. Expression of huntingtin interacting protein-1 (HIP-1) gene was found to be increased not only in PG cells but also in fibroblast cells from aged healthy donors. Thus, HIP-1 might be a molecular assistant in the pathogenesis of the cellular senescent process in the human cells tested.
Department of Plastic Surgery, Graduate School of Medicine, Chiba University, Japan.
Shinichi
S
Chigira
Katsuo
K
Sugita
Kazuko
K
Kita
Shigeru
S
Sugaya
Yoshiko
Y
Arase
Masaharu
M
Ichinose
Hiroshi
H
Shirasawa
Nobuo
N
Suzuki
D000293:Adolescent; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D016922:Cellular Senescence; D002648:Child; D004268:DNA-Binding Proteins; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D027383:Papillomaviridae; D011371:Progeria / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019098:Telomerase / Q000378:metabolism; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C073368:HIP1 protein, human; D012333:RNA, Messenger; D019098:Telomerase; D001616:beta-Galactosidase