A novel extracellular protein of Streptomyces peucetius binds to daunorubicin but does not inhibit the bioactivity of the antibiotic.
Extracellular proteins of Streptomyces peucetius that bind to a red pigment were identified during the course of isolation of mutants defective in daunorubicin production. Two pigment-protein complexes were partially purified and this complex inhibited the growth of Bacillus subtilis. Routine solvent extraction could not separate the pigment from purified pigment-protein complex. Treatment with 2% SDS at 100 degrees C followed by solvent extraction also failed to separate the protein from the pigment. However, the pigment could be separated from the purified pigment-protein complex by heating it in 0.1M HCl at 100 degrees C followed by solvent extraction. The pigment extracted from the complex was analyzed by HPLC and found to be daunorubicin.
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India.
Ranjan
R
Prasad
V
V
Sasikala
K S
KS
Vetrivel
K
K
Dharmalingam
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003630:Daunorubicin / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003630:Daunorubicin