Genetic analysis of multiple sclerosis in Europeans: French data.
We report the results of a genome-wide screen for linkage disequilibrium (LD) in multiple sclerosis (MS) performed on 200 cases, 200 controls and 200 case-parent trios from France employing pooled DNA methodology. A total of 3510 microsatellite markers supplied through the GAMES collaborative were analysed and ranked according to their evidence for association. The most promising 117 markers were then followed up in a two-step validation process. In the first step, additional PCR of the DNA pools was performed in order to refine the ranking order. In the second step, markers were genotyped in individual cases and parents from the trio families. Seven markers showing nominally significant allele frequency differences between affected and unaffected emerged-D6S265, D12S1064, TNFa, D7S1824, D14S1426, D14S605 and D21S2051. These potential associations will require confirmation in further studies.
Laboratoire d'Immunologie, UPRES EA 1257 (IFR97), Faculté de Médecine, 2 Avenue du Pr Léon Bernard CS 34317, 35043 Rennes Cedex, France.
Mehdi
M
Alizadeh
Emmanuelle
E
Génin
Marie-Claude
MC
Babron
Brigitte
B
Birebent
Isabelle
I
Cournu-Rebeix
Jacqueline
J
Yaouanq
Stéphane
S
Dréano
Stephen
S
Sawcer
Alastair
A
Compston
Michel
M
Clanet
Gilles
G
Edan
Bertrand
B
Fontaine
Françoise
F
Clerget-Darpoux
Gilbert
G
Semana
D000483:Alleles; D016022:Case-Control Studies; D005602:France / Q000453:epidemiology; D005787:Gene Frequency; D005820:Genetic Testing / Q000379:methods* / Q000706:statistics & numerical data; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D007391:International Cooperation; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D009103:Multiple Sclerosis / Q000453:epidemiology / Q000235:genetics*; D011446:Prospective Studies