Enhanced bone-to-implant contact by platelet-released growth factors in mandibular cortical bone: a histomorphometric study in minipigs.
OBJECTIVE
To determine the effects of platelet-released growth factors (PRGF) on bone-to-implant contact (BIC) in minipig cortical bone.


METHODS
In each of 8 adult minipigs, 2 implants were placed in the facial wall of the mandible, one implant with and one without PRGF. PRGF was defined as the supernatant from washed, thrombin-activated, allogenic, platelet-rich plasma cells obtained after centrifugation. Four animals were sacrificed at 4 weeks, and the remaining 4 were sacrificed at 8 weeks. For histomorphometric analyses, undecalcified ground specimens were prepared and stained with the Levai-Laczko stain.


RESULTS
For the entire follow-up time, 55.30% BIC was found with PRGF versus 38.91% without PRGF (P = .0198). At 4 weeks, BIC was 44.20% with PRGF versus 29.62% without PRGF (P = .0632), and at 8 weeks there was 70.36% BIC with PRGF versus 48.20% without PRGF (P = .1221).


CONCLUSIONS
Growth factors and other molecules released upon activation of platelet-rich plasma cells can enhance implant anchorage in cortical bone. PRGF obtained from allogenic sources does not impair healing.


CONCLUSIONS
The results indicated that a single application of PRGF before implant placement can be sufficient to increase the percent BIC in minipig cortical bone.
Department of Oral Surgery, School of Dentistry, University of Vienna, Austria. gabor.fuerst@univie.ac.at
Gabor
G
Fuerst
Reinhard
R
Gruber
Stefan
S
Tangl
Fidel
F
Sanroman
Georg
G
Watzek
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D015921:Dental Implants; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005615:Freezing; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D006133:Growth Substances / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000473:pathology*; D016348:Osseointegration / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects; D017713:Platelet Transfusion; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology
D015921:Dental Implants; D006133:Growth Substances