Synthesis of novel fluorescent probes for the molecular chaperone Hsp90.
Heat shock protein 90 (Hsp90) is a molecular chaperone necessary for maintaining oncogenic transformation. There is substantial interest in developing novel agents that bind to the N-terminal of the chaperone. Here we report the synthesis and characterization of two fluorescent Hsp90 inhibitors and probe their use in an Hsp90 fluorescent polarization assay.
Program in Cell Biology and Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Laura
L
Llauger-Bufi
Sara J
SJ
Felts
Henri
H
Huezo
Neal
N
Rosen
Gabriela
G
Chiosis
D001667:Binding, Competitive; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000187:drug effects; D007700:Kinetics; D015394:Molecular Structure; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins