Spectrophotometric determination of procaine hydrochloride in pharmaceutical products using 1,2-naphthoquinone-4-sulfonic acid as the chromogenic reagent.
Spectrophotometric determination of procaine hydrochloride is described. The procaine hydrochloride reacts with 1,2-naphthoquinone-4-sulfonic acid in pH 3.60 buffer solution to form a salmon pink compound, and its maximum absorption wavelength is at 484 nm, epsilon(484)=5.22 x 10(3).The absorbance for procaine hydrochloride from 0.30 to 100 microg ml(-1) obeys Beer's law. The linear regression equation of the calibration graph is C=19.23A-0.03, with a linear regression correlative coefficient is 0.9996, the detection limit is 0.28 microg ml(-1); recovery is from 98.0 to 105.2%. Effects of pH, surfactant, organic solvent, foreign ions, and standing time on the determination of procaine hydrochloride have been examined. This method is rapid and simple, and can be used for the determination of procaine hydrochloride in injection solution of procaine hydrochloride. The results obtained by this method agreed with those by the official method (dead-stop titration).
Xu
Li Xiao
LX
Department of Chemistry, Shandong Normal University, Jinan 250014, China.
Shen
Yun Xiu
YX
Wang
Huai You
HY
Jiang
Ji Gang
JG
Xiao
Yan
Y
D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011343:Procaine / Q000032:analysis*; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations; D012997:Solvents; C011942:1,2-naphthoquinone-4-sulfonate; D011343:Procaine