[Tumor necrosis factor-alpha enhances the effect of endothelin on renal vasoconstriction in isolated perfused rat kidney].
OBJECTIVE
To explore the role of tumor necrosis factor-alpha (TNF-alpha) in the pathogenesis of hepatorenal syndrome.


METHODS
By isolated perfused kidney technique, rat kidneys were perfused at a constant flow. Changes in perfusion pressure (mmHg) were consecutively measured with multi-functional physiology recorder. After TNF-alpha or heparin treated 90 minutes, the perfusion pressure stimulated by endothelin-1 (ET-1) was detected.


RESULTS
TNF-alpha and heparin didn't modify the baseline perfusion pressure. When ET-1 was added at 2 nmol/L, the perfusion pressures increased to (47+/-9) mmHg, (97+/-36) mm Hg and (11+/-6) mm Hg in control, TNF-alpha and heparin (10mg/L) treated group, respectively, which were different among the three groups (t>or=3.811, P<0.01). No pathological damages were found in kidney tissues from all the groups after being stained with hematoxylin/eosin.


CONCLUSIONS
TNF-alpha plays an important role in the pathogenesis of hepatorenal syndrome by promoting renal vasoconstriction.
Affiliated Second Hospital, China Medical University, Shenyang 110004, China.
Ying
Y
Wen
Jing-yan
JY
Wang
Pei
P
Liu
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006530:Hepatorenal Syndrome / Q000209:etiology* / Q000378:metabolism; D066298:In Vitro Techniques; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012077:Renal Artery / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects*
D015220:Calcium Channels; D019332:Endothelin-1; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D014409:Tumor Necrosis Factor-alpha; D006493:Heparin