Recovery of surface bacteria from and surface sanitization of cantaloupes.
Practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. Cantaloupes (Cucumis melo L. var. reticulates) were immersed in an inoculum containing Salmonella enterica serovar Poona or Pantoea agglomerans at ca. 10(4) to 10(5) CFU/ml. An efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. The method consisted of washing the entire melon with Butterfield's buffer containing 1% Tween 80 in a plastic bag placed inside a plastic pail affixed to an orbital shaker. Levels of S. enterica Poona recovered by washing the entire melon were significantly higher than those recovered by the more common laboratory method of blending the rind. P. agglomerans can be used as a non-pathogenic proxy for S. enterica Poona. A three-compartment surface sanitization method consisting of washing with an antimicrobial soap solution, scrubbing with a brush in tap water, and immersion in 150 ppm of sodium hypochlorite reduced the initial level of recoverable viable bacteria by 99.8%. When examined separately, scrubbing with a vegetable brush in tap water, washing with soap, and dipping in chlorine were found to reduce the bacterial load by 70, 80, and 90%, respectively.
Produce Safety and Microbiology, US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, California 94710, USA. jbarak@pw.usda.gov
Jeri D
JD
Barak
Bryan
B
Chue
Daniel C
DC
Mills
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D028463:Cucumis melo / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D006801:Humans; D019779:Salmonella enterica / Q000187:drug effects* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
D000891:Anti-Infective Agents, Local