Effect of naturally occurring monoterpenes carvone, limonene and perillic acid in the inhibition of experimental lung metastasis induced by B16F-10 melanoma cells.
The effects of naturally occurring monoterpenes on lung metastasis induced by B16F-10 melanoma cells were studied in C57BL/6 mice. Administration of monoterpenes such as limonene (100 micromoles/kg body wt. 10 doses i.p.) and perillic acid (50 micromoles/kg body wt 10 doses i.p.) remarkably reduced the metastatic tumour nodule formation by 65% and 67%, respectively. These results correlated with the biochemical parameters such as serum sialic acid, lung collagen hydroxyproline and uronic acid contents. Serum sialic acid level in control group was 126.8 microg/ml serum which was significantly lowered in limonene (49.3 microg/ml serum) and perillic acid treated animals (53.6 microg/ml serum). Uronic acid level was also inhibited to 56% and 39.7% in limonene and perillic acid treated animals, respectively. Histopathological studies also correlated with these above results. Administration of Carvone even at 100 micromoles/kg body wt. did not have any significant effect on the metastatic tumour growth. These results indicate that limonene and perillic acid could inhibit the metastatic progression of B16F-10 melanoma cells in mice.
Amala Cancer Research Centre, Amala Nagar, Kerala, India.
T J
TJ
Raphael
G
G
Kuttan
D000818:Animals; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D006595:Hexosamines / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D000077222:Limonene; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000556:secondary*; D008297:Male; D008545:Melanoma / Q000097:blood / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D039821:Monoterpenes / Q000008:administration & dosage / Q000627:therapeutic use*; D019158:N-Acetylneuraminic Acid / Q000097:blood; D009368:Neoplasm Transplantation; D015996:Survival Rate; D013729:Terpenes / Q000008:administration & dosage / Q000627:therapeutic use*; D014574:Uronic Acids / Q000378:metabolism
D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D006595:Hexosamines; D039821:Monoterpenes; D013729:Terpenes; D014574:Uronic Acids; C006923:carvone; C078706:perillic acid; D003094:Collagen; D000077222:Limonene; D019158:N-Acetylneuraminic Acid; D006909:Hydroxyproline