Reduced accumulation of specific microRNAs in colorectal neoplasia.
Short non-coding RNAs are known to regulate cellular processes including development, heterochromatin formation, and genomic stability in eukaryotes. Given the impact of these processes on cellular identity, a study was undertaken to investigate possible changes in microRNA (miRNA) levels during tumorigenesis. A total of 28 different miRNA sequences was identified in a colonic adenocarcinoma and normal mucosa, including 3 novel sequences and a further 7 that had previously been cloned only from mice. Human homologues of murine miRNA sequences, miR-143 and miR-145, consistently display reduced steady-state levels of the mature miRNA at the adenomatous and cancer stages of colorectal neoplasia.
Michael
Michael Z
MZ
Department of Gastroenterology and Hepatology, Flinders Centre for Digestive Health, Flinders Medical Centre, South Australia, Australia. michael.michael@flinders.edu.au
O' Connor
Susan M
SM
van Holst Pellekaan
Nicholas G
NG
Young
Graeme P
GP
James
Robert J
RJ
D000230:Adenocarcinoma / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D035683:MicroRNAs / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data
D035683:MicroRNAs