High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging.
Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and a femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in a new operating regime in which a short coherence gate is used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular imaging is demonstrated in the Xenopus laevis tadpole and in human skin with a 3-microm coherence gate and a 30-microm confocal gate. The ability to achieve cellular imaging with a lower numerical aperture should facilitate the development of miniaturized probes for in vivo imaging applications.
Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
A D
AD
Aguirre
P
P
Hsiung
T H
TH
Ko
I
I
Hartl
J G
JG
Fujimoto
D000818:Animals; D002477:Cells / Q000166:cytology*; D004867:Equipment Design; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation; D012867:Skin / Q000166:cytology; D041623:Tomography, Optical Coherence / Q000295:instrumentation; D014982:Xenopus laevis