Effects of endocrine disrupting chemicals on distinct expression patterns of estrogen receptor, cytochrome P450 aromatase and p53 genes in oryzias latipes liver.
Expression pattern analysis of three genes, i.e., the estrogen receptor (ER), cytochrome P450 aromatase (CYP19), and p53 genes, in Japanese Medaka liver was studied in the presence of 17beta-estradiol, nonylphenol, and bisphenol A. Using the distinct expression patterns of these three genes, the different transcriptional mechanisms by endocrine disrupting chemicals (EDCs), and the stability of the transcripts of each gene, were examined. In tests done with both male and female Medaka over 10 days, it was found that each gene had a specific expression pattern. The expression of the ER gene increased rapidly for the first 2 days and then leveled out and maintained a stable expression level, while the CYP19 gene expression showed a fairly consistent increase in the expression levels after exposure. In contrast to both these genes, the p53 gene expression levels reached a maximum value within 2 days and then gradually decreased for the remainder of the experiment. These findings suggest that different expression mechanisms may exist for these genes in Japanese Medaka in response to the presence of EDCs.
National Research Laboratory on Environmental Biotechnology, Kwangju Institute of Science and Technology (K-JIST); 1 Oryong-dong, Puk-gu, Kwangju 500-712, South Korea.
Jiho
J
Min
Sung-Kyu
SK
Lee
Man Bock
MB
Gu
D000818:Animals; D001141:Aromatase / Q000096:biosynthesis*; D001559:Benzhydryl Compounds; D004703:Endocrine System / Q000187:drug effects; D004958:Estradiol / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009990:Oryzias; D010636:Phenols / Q000633:toxicity; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D000098826:Bisphenol A Compounds
D001141:Aromatase; D001559:Benzhydryl Compounds; D004958:Estradiol; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds