Sequence of HA gene of avian influenza A/Chicken/Guangdong/SS/1994 (H9N2) virus.
The RNA of the hemagglutinin (HA) gene of A/Chicken/Guangdong/SS/1994 (H9N2) was reverse transcription-polymerase chain reaction amplified, and the cDNA was cloned into a plasmid vector. The complete coding sequence of the HA gene was sequenced and included 1683 nucleotides, which encoded for a protein of 560 amino acids. The potential glycosylation sites related to HA protein function were highly conserved. The amino acid sequence of the HA proteolytic cleavage was G-S-S-R/G. This cleavage site sequence is compatible with a low-pathogenic avian influenza virus. Sequence comparison of this HA gene with other H9 influenza virus sequences in the GenBank database showed a 82%-97% nucleotide and amino acid sequence similarity.
Department of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002681:China; D003001:Cloning, Molecular; D005814:Genes, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype; D009980:Influenza A virus / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000235:genetics
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral; D011994:Recombinant Proteins; D015678:Viral Structural Proteins