[Changes of osteocalcin in bone and bone marrow in tail suspended rats].
OBJECTIVE
To study osteocalcin [correction of osteocakin] (OC) changes in bone and marrow and calcium deposition in bone and cartilage under simulated weightlessness.


METHODS
Twenty SD rats were randomly divided into 14 d and 28 d tail suspension group and 2 corresponding control groups. Histological samples were in situ hybridized and trichrome stained.


RESULTS
OC expression of bone and marrow of rats were lower in tail suspended rats than that in the control (P<0.05). OC expression in 14 d tail suspended rats were higher than that in 28 d tail suspended group (P<0.05). Mineralization was inhibited, and demineralization of femur [correction of furmer] and cartilage mineralized matrix was prominent. Demineralization was more prominent in 28 d group.


CONCLUSIONS
OC levels in bone and marrow of rats were lower after tail suspension. Calcium deposition was inhibited in bone and cartilage. Demineralization was prominent after long term hindlimb unloading.
Fu
Chong-jian
CJ
Department of Stomatology, Jinan Military General Hospital, Jinan, China.
Yu
Bing-bing
BB
Yang
Lian-jia
LJ
Zhang
Li-fan
LF
D000818:Animals; D018488:Bone Demineralization, Pathologic / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D015519:Bone Density / Q000502:physiology; D001853:Bone Marrow / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism / Q000503:physiopathology; D005269:Femur / Q000378:metabolism / Q000503:physiopathology*; D019417:Hindlimb Suspension; D015675:Osteocalcin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018474:Weightlessness Simulation / Q000009:adverse effects*
D015675:Osteocalcin; D002118:Calcium