Determination of aristolochic acids by capillary electrophoresis.
A method for the separation and quantification of aristolochic acids by capillary electrophoresis is described. Buffer solutions composed of sodium dihydrogen phosphate, sodium borate, and sodium dodecyl sulfate at pH 6.5-7 were found to be suitable for the separation of aristolochic acids, which can be well resolved in a few minutes. The separation and identification of six aristolochic acids contained in a commercial tincture called charrua, a traditional herbal medicine consisting of a hydroalcoholic extract of Aristolochia argentina, corroborate the usefulness of the method. The eventual toxic properties of the aristolochic acid containing charrua tincture are discussed.
Priestap
Horacio A
HA
Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Iglesias
Silvia L
SL
Desimone
Martín F
MF
Diaz
Luis E
LE
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D034341:Aristolochic Acids; D010936:Plant Extracts