[Localization of cohesin complexes of polytene chromosomes of Drosophila melanogaster located on interbands].
The distribution of cohesin complex in polytene chromosomes of Drosophila melanogaster was studied. Cohesin is a complicated protein complex which is regulated by the DRAD21 subunit. Using immunostaining for DRAD21p, the cohesins were shown to be preferentially located in the interband regions. This specificity was not characteristic for puffs, where uniform staining was observed. The presence of a few brightly fluorescent regions (five to ten per chromosome arm) enriched with cohesin complexes was shown. Some of these regions had permanent location, and the others, variable location. No antibody binding was detected in the chromocenter. Immunostaining of interphase nuclei of neuroblasts revealed large cohesin formations. On the polytene chromosomes of D. melanogaster, the Drad21 gene was mapped to the chromocentric region (81) of the L arm of chromosome 3.
Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 199034 Russia.
A V
AV
Markov
A A
AA
Zakharov
A P
AP
Galkin
A V
AV
Strunnikov
A F
AF
Smirnov
D000818:Animals; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D005656:Fungal Proteins; D017404:In Situ Hybridization, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics*; D000097722:Cohesins
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; D000097722:Cohesins