The acute response of neutrophil function to a bout of judo training.
Intensive exercise training decreases neutrophil functions in athletes. However, no studies to date have investigated the effect of irregular-interval training, such as is associated with judo training programmes, on neutrophil functions. The purpose of this study was to examine such effects. Thirty-seven male college judoists participated in this study. Neutrophil oxidative burst activity, phagocytic activity and expression of CD11b and CD16 per cell were measured by fl ow cytometry before and after judo training. Total neutrophil counts increased significantly from 2.98 +/- 0.82 to 7.95 +/- 1.80 x 10(3)/ microL (p < 0.001). The proportion of neutrophils producing reactive oxygen species (ROS) was increased significantly (p < 0.001). On the other hand, the phagocytic activity decreased after training, as shown by a decrease in the amount of ingested opsonized zymosan per cell (p < 0.001), possibly as a compensatory effect for the increased numbers of ROS-producing neutrophils. Expression of CD11b and CD16 per cell decreased by 20% and 30%, respectively, after judo training. In conclusion, judo training induced a decrease in phagocytic activity through the lowered expression of CD11b and CD16 on the surface of neutrophils, and increased the oxidative burst activity of neutrophils.
Department of Hygiene, Hirosaki University School of Medicine, Hirosaki, Aomori, Japan.
Daisuke
D
Chinda
Takashi
T
Umeda
Tadashi
T
Shimoyama
Arata
A
Kojima
Masaru
M
Tanabe
Shigeyuki
S
Nakaji
Kazuo
K
Sugawara
D000293:Adolescent; D039481:CD11b Antigen / Q000096:biosynthesis; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D016139:Martial Arts / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D010806:Physical Education and Training; D017452:Receptors, IgG / Q000096:biosynthesis; D016897:Respiratory Burst / Q000502:physiology
D039481:CD11b Antigen; D017452:Receptors, IgG