Mitochondrial DNA control region diversity in hairs and body fluids of monozygotic triplets.
Length heteroplasmy of the homopolymeric cytosine stretch in the hypervariable region II of the mitochondrial D-loop was investigated in blood, buccal cells and hair shafts of monozygotic triplets. The proportions of length heteroplasmy were determined by cloning and sequencing of multiple independent clones. Blood and buccal cells showed an accumulation of molecules with one and two insertions of cytosine residues in relation to the Cambridge Reference Sequence (CRS). The results did not show statistically significant differences between blood and buccal cells of one and the same individual and also not between the three monozygotic brothers. In the hair samples a loss of cytosine residues was established in all three monozygotic individuals compared to blood and buccal cells, suggesting that this must be a regular process. Furthermore, the hair shaft samples showed significant differences between the frequencies of 7, 8 or 9 Cs in the poly C region comparing the three individuals (p<0.008) and in addition there were highly significant differences (p<0.0001) when comparing the results for six different hairs of each individual separately. From these results it can be assumed that besides a common genetic bottleneck during embryonic development, a post-embryonic bottleneck seems to exist in each hair follicle.
Institute of Legal Medicine, University of Münster, Röntgenstrasse 23, 48149 Muenster, Germany. pfeiffh@uni-muenster.de
Heidi
H
Pfeiffer
Sabine
S
Lutz-Bonengel
Stefan
S
Pollak
Rolf
R
Fimmers
Max P
MP
Baur
Bernd
B
Brinkmann
D000328:Adult; D001769:Blood; D001826:Body Fluids / Q000502:physiology*; D022801:Complementarity Determining Regions / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005554:Forensic Medicine; D006197:Hair / Q000502:physiology*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012463:Saliva; D014310:Triplets / Q000235:genetics*; D014430:Twins, Monozygotic / Q000235:genetics*
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial