Human fibrillarin forms a sub-complex with splicing factor 2-associated p32, protein arginine methyltransferases, and tubulins alpha 3 and beta 1 that is independent of its association with preribosomal ribonucleoprotein complexes.
Fibrillarin (FIB, Nop1p in yeast) is an RNA methyltransferase found not only in the fibrillar region of the nucleolus but also in Cajal bodies. FIB is essential for efficient processing of preribosomal RNA during ribosome biogenesis, although its precise function in this process and its role in Cajal bodies remain uncertain. Here, we demonstrate that the human FIB N-terminal glycine- and arginine-rich domain (residues 1-77) and its spacer region 1 (78-132) interact with splicing factor 2-associated p32 (SF2A-p32) and that the FIB methyltransferase-like domain (133-321) interacts with protein-arginine methyltransferase 5 (PRMT5, Janus kinase-binding protein 1). We also show that these proteins associate with several additional proteins, including PRMT1, tubulin alpha 3, and tubulin beta 1 to form a sub-complex that is principally independent of the association of FIB with preribosomal ribonucleoprotein complexes that co-immunoprecipitate with the sub-complex in human cells expressing FLAG-tagged FIB. Based on the physical association of FIB with SF2A-p32 and PRMTs, as well as the other reported results, we propose that FIB may coordinate both RNA and protein methylation during the processes of ribosome biogenesis in the nucleolus and RNA editing such as small nuclear (nucleolar) ribonucleoprotein biogenesis in Cajal bodies.
Yanagida
Mitsuaki
M
Department of Applied Biological Science, United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509.
Hayano
Toshiya
T
Yamauchi
Yoshio
Y
Shinkawa
Takashi
T
Natsume
Tohru
T
Isobe
Toshiaki
T
Takahashi
Nobuhiro
N
D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D020541:Coiled Bodies / Q000737:chemistry; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry*; D011496:Protein Methyltransferases / Q000737:chemistry*; D011484:Protein-Arginine N-Methyltransferases; D012313:RNA / Q000737:chemistry; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000737:chemistry*; D000068103:Serine-Arginine Splicing Factors; D014404:Tubulin / Q000737:chemistry*; D014461:Ultracentrifugation
D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D014404:Tubulin; C047458:fibrillarin; D000068103:Serine-Arginine Splicing Factors; D012313:RNA; D011496:Protein Methyltransferases; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases