Towards the development of an integrated capillary electrophoresis optical biosensor.
Extending the previous preliminary study on the construction of a capillary electrophoresis (CE)/sensor for the detection of reducing analytes, we focus the interest on the simultaneous detection of redox active species, which are important indicators of the oxidative damage in tissues, of food preservation, and of pollution. The CE/sensor was built by modifying the detector-portion of the capillary with the redox-sensitive polymer polyaniline (PANI). The analyte is detected by monitoring the changes in optical absorption of the PANI film. The CE/sensor was tested, with good results, with ascorbic acid, glutathione (GSH), as well as with compounds with very close similarity (ascorbic and isoascorbic acid). The kinetics of oxidation and reduction of PANI were evaluated. Further a PANI/CE-biological sensor was developed by coupling an enzyme, glucose oxidase (GOD), to the PANI-modified portion of the capillary. The stability of the immobilized GOD and the sensitivity of the CE/biosensor were studied, by using glucose as test analyte in concentrations within the physiological range. The results indicate that the CE/biosensor had good stability (more than 75% of original activity retained after 30 operational days), manufacturing reproducibility and a sensing range convenient for monitoring physiological glucose (1-24 mM).
Bossi
Alessandra
A
Department of Science and Technology, University of Verona, Verona, Italy. bossi@sci.univr.it
Castelletti
Laura
L
Piletsky
Sergey A
SA
Turner
Anthony P F
AP
Righetti
Pier Giorgio
PG
D000814:Aniline Compounds; D001205:Ascorbic Acid / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000592:standards; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D007700:Kinetics; D010084:Oxidation-Reduction
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D005949:Glucose Oxidase; D005978:Glutathione; D005947:Glucose; D001205:Ascorbic Acid