Anticarcinogenic properties of a daidzein-rich fraction isolated from soybean.
In a previous study, we demonstrated that the methanol extract of soybean powder contains an active component(s) that promotes the differentiation of HL-60 cells. Partial purification of the extract, using solvent fractionation and silica gel chromatography, produced an active fraction rich in daidzein. The daidzein-rich fraction (DRF) was evaluated for its cancer preventive potential by assessing its cytotoxic activity and effect on the expression of the transforming growth factor beta (TGF-beta) family of cytokines and their receptors. DRF appeared to exert cytotoxic activity via an apoptotic pathway as evaluated by a DNA fragmentation assay and caspase-3 induction. DRF also increased the expression of TGF-beta2, but had little effect on the expression of other members of the TGF-beta family of cytokines and their receptors, or on the expression of the vascular endothelial growth factor gene. In conclusion, the DRF isolated from the methanol extract of soybean may have the potential to prevent tumorigenesis and, therefore, deserves to undergo further evaluation of its active component(s) and in vivo evaluation for anticarcinogenic efficacy.
Lee
Jeong Soon
JS
Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, Korea.
Son
Kun-Ho
KH
Sung
Mi-Kyung
MK
Kim
Young Kyoon
YK
Yu
Rina
R
Kim
Jong-Sang
JS
D000818:Animals; D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D053938:DNA Fragmentation; D004790:Enzyme Induction / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D013025:Glycine max / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000378:metabolism*
D016588:Anticarcinogenic Agents; D007529:Isoflavones; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C004742:daidzein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases