Characterization of immunomodulatory polysaccharides from Salvia officinalis L.
Crude polysaccharide fractions, rich mainly in arabinogalactans (A), pectin (B) and glucuronoxylan-related polymers (D), have been obtained from aerial parts of sage (Salvia officinalis L.) by sequential extraction with various reagents. Arabinogalactans displayed on HPLC a dominance of lower molecular-mass polymers (MW < 10,000), while pectin and glucuronoxylan-related polysaccharides showed predominance of polymers with MW > 50,000. Individual polysaccharide fractions were examined for their immunomodulatory activity in the in vitro comitogenic thymocyte test. The polysaccharide fractions tested possessed the capacity to induce rat thymocyte proliferation in the order D>B>A. Besides, fraction D possessed a significant comitogenic effect, and the SIcomit/SImit ratio 3-4 indicates potential adjuvant properties of this glucuronoxylan-rich material.
Capek
P
P
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. chemcape@savba.sk
Hríbalová
V
V
Svandová
E
E
Ebringerová
A
A
Sasinková
V
V
Masarová
J
J
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001671:Biochemistry / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005685:Galactans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008934:Mitogens / Q000276:immunology / Q000494:pharmacology; D008970:Molecular Weight; D010368:Pectins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027543:Salvia officinalis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D014990:Xylans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D005685:Galactans; D008934:Mitogens; D011134:Polysaccharides; D014990:Xylans; C038910:glucuronoxylan; D010368:Pectins; C005653:arabinogalactan