Targets of the cyclin-dependent kinase Cdk1.
The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have been identified. Here, we screened a budding yeast proteomic library for proteins that are directly phosphorylated by Cdk1 in whole-cell extracts. We identified about 200 Cdk1 substrates, several of which are phosphorylated in vivo in a Cdk1-dependent manner. The identities of these substrates reveal that Cdk1 employs a global regulatory strategy involving phosphorylation of other regulatory molecules as well as phosphorylation of the molecular machines that drive cell-cycle events. Detailed analysis of these substrates is likely to yield important insights into cell-cycle regulation.
Department of Physiology, University of California, San Francisco, California 94143, USA.
Jeffrey A
JA
Ubersax
Erika L
EL
Woodbury
Phuong N
PN
Quang
Maria
M
Paraz
Justin D
JD
Blethrow
Kavita
K
Shah
Kevan M
KM
Shokat
David O
DO
Morgan
D000255:Adenosine Triphosphate / Q000378:metabolism; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002453:Cell Cycle; D016384:Consensus Sequence; D010766:Phosphorylation; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D016203:CDC2 Protein Kinase