[Comparative stability assessment of laccases from the basidiomycetes Coriolus hirsutus and Coriolus zonatus in the presence of effectors].
Stability characteristics of the laccases of the basidiomycetes Coriolus hirsutus and Coriolus zonatus were measured comparatively at temperatures 25 and 40 degrees C in the presence of various effectors (proteins, salts, polyalcohols, polyacids, and polyelectrolytes). Stabilization effects of cations on the laccases from C. hirsutus and C. zonatus decreased in the descending series Cu2+ > Mg2+ > Ca2+ and Ca2+ > Mg2+ > Mn2+, respectively. Tween 20 caused insignificant stabilization of the two enzymes. The C. zonatus laccase was also insignificantly stabilized as a result of treatment with bovine serum albumin. The enzymatic activity of the laccase preparations from C. hirsutus and C. zonatus was conserved virtually completely after vacuum drying (84 and 93%, respectively). The most effective stabilizer of the C. hirsutus laccase was found to be dextran (17 kD). Dry preparations treated with this agent conserved up to 95% of the enzymatic activity. The most effective stabilizer of the C. zonatus was polyacrylic acid (102% of the initial activity).
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071 Russia.
E V
EV
Stepanova
O V
OV
Koroleva
V P
VP
Gavrilova
E O
EO
Landesman
A
A
Makower
D B
DB
Papkovskiĭ
D000180:Acrylic Resins / Q000494:pharmacology; D001487:Basidiomycota / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005612:Freeze Drying; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D011136:Polysorbates / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013045:Species Specificity; D013696:Temperature
D000180:Acrylic Resins; D003911:Dextrans; D011136:Polysorbates; D012710:Serum Albumin, Bovine; D008345:Manganese; C006903:carbopol 940; D003300:Copper; D042845:Laccase; D008274:Magnesium; D002118:Calcium