Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus.
The rapid development of anthelmintic resistance, associated with the high cost of the available anthelmintic drugs, had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plants as alternative sources of anthelmintics. Spigelia anthelmia extracts obtained with hexane, chloroform, ethyl acetate or methanol, were tested on Haemonchus contortus eggs and larvae via egg hatch and larval development tests. The extracts were evaluated at five concentrations: 3.1, 6.2, 12.5, 25.0 and 50.0 mg ml(-1). At 50.0 mg ml(-1), the ethyl acetate extract inhibited 100% of the egg hatching and 81.2% of the larval development. In a similar way the methanolic extract inhibited 97.4% of the egg hatching and 84.4% of larval development. These results suggest that utilization of S. anthelmia extracts may be useful in the control of sheep and goats gastrointestinal nematodes.
Assis
L M
LM
Faculdade de Veterinária, Universidade Estadual do Ceará, Av Paranjana 1700, Fortaleza, Ceara 60740-000, Brazil.
Bevilaqua
C M L
CM
Morais
S M
SM
Vieira
L S
LS
Costa
C T C
CT
Souza
J A L
JA
D000818:Animals; D004305:Dose-Response Relationship, Drug; D015511:Goat Diseases / Q000188:drug therapy / Q000469:parasitology; D006041:Goats; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000254:growth & development; D066298:In Vitro Techniques; D007814:Larva / Q000187:drug effects; D029541:Loganiaceae / Q000737:chemistry*; D010063:Ovum / Q000187:drug effects; D008517:Phytotherapy / Q000662:veterinary; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology; D016896:Treatment Outcome
D010936:Plant Extracts