Transformation of the white-rot basidiomycete Coriolus hirsutus using the ornithine carbamoyltransferase gene.
An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10(3)-10(4) transformants per mug DNA per 10(6)-10(7) oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg+ phenotype of the transformants was stably inherited through mitosis.
Tsukamoto
Akira
A
Advanced Technology Research Laboratory, Oji Paper Co., Ltd., 10-6 Shinonome 1-chome, Tokyo 135-8558, Japan.
Kojima
Yasushi
Y
Kita
Yukio
Y
Sugiura
Jun
J
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D015139:Blotting, Southern; D002875:Chromosomes; D003001:Cloning, Molecular; D018628:Gene Dosage; D008969:Molecular Sequence Data; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D014170:Transformation, Genetic
D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D009954:Ornithine Carbamoyltransferase