Complete nucleotide sequence of an isolate of the nepovirus raspberry ringspot virus from grapevine.
The complete nucleotide sequence of the RNAs 1 and 2 of the nepovirus Raspberry ringspot virus cherry isolate (RpRSV-ch) from grapevine was determined. The RNA 1 is 7935 nucleotides (nt) long excluding the poly(A) tail, and contains one long open reading frame (ORF) encoding a polypeptide of 2367 amino acids. This ORF is preceeded by a 136nt 5' non-coding region, and followed by a 695nt 3' non-coding region. Conserved amino acid motifs, characteristic of the viral protease cofactor, the NTP-binding protein, proteinase and polymerase, were found in the sequence of the RNA 1-encoded polyprotein. The RNA 2 is 3915nt long excluding the poly(A) tail, and contains one long ORF encoding a polypeptide of 1106 amino acids. This ORF is preceeded by a 203nt 5' non-coding region, and followed by a 390nt 3' non-coding region. When compared to the corresponding sequences of other nepoviruses, a maximum level of 34% identity was found between the RNA 1-encoded polypetides of RpRSV-ch and other nepoviruses. For the RNA 2-encoded polypeptide, 88% identity was found between RpRSV-ch and RpRSV-S, a Scottish isolate of RpRSV from raspberry, and a maximum 29% identity between RpRSV-ch and other nepoviruses.
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Centrum Gruene Gentechnik, DLR Rheinpfalz, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
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D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D020816:Amino Acid Motifs / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames / Q000235:genetics; D020815:Polyproteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics; D027843:Vitis / Q000821:virology*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins