Automated sleep staging in rat with a standard spreadsheet.
A new method of automated sleep-wake staging in the rat is described. Hippocampal electroencephalographic (HPC) and nuchal electromyographic signals were recorded by a digital polygraph. The HPC channel was filtered off-line to obtain the original plus theta and delta waves. Statistics of each of these four channels were obtained every 5 s and exported to a standard spreadsheet. The automated staging consisted of five steps: (1) automatic detection of waking, nonrapid eye movement sleep and rapid eye movement sleep patterns (5-s periods); (2) calculation of statistics for each vigilance state; (3) final classification of 5-s periods; (4) construction of a primary 20-s epoch hypnogram; and (5) automatic refinement of the previous hypnogram. The system includes indices about the accuracy of the staging and was validated with five recordings of 23 h each. The global agreement between human and automatic scoring in the validation recordings was 94.32%.
Institut de Neurociències, Dept. Psicobiologia i Metodologia de les Ciències de la Salut, Facultat de Psicologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. david.costa@uab.es
David
D
Costa-Miserachs
Isabel
I
Portell-Cortés
Meritxell
M
Torras-Garcia
Ignacio
I
Morgado-Bernal
D000818:Animals; D001143:Arousal / Q000502:physiology; D003626:Data Display; D004567:Electrodes, Implanted; D004569:Electroencephalography / Q000295:instrumentation; D004576:Electromyography; D006624:Hippocampus / Q000502:physiology; D008297:Male; D008838:Microcomputers; D017286:Polysomnography / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012890:Sleep / Q000502:physiology; D012894:Sleep Stages / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology; D013238:Stereotaxic Techniques; D014851:Wakefulness / Q000502:physiology