Enhanced bone-to-implant contact by platelet-released growth factors in mandibular cortical bone: a histomorphometric study in minipigs.
OBJECTIVE
To determine the effects of platelet-released growth factors (PRGF) on bone-to-implant contact (BIC) in minipig cortical bone.


METHODS
In each of 8 adult minipigs, 2 implants were placed in the facial wall of the mandible, one implant with and one without PRGF. PRGF was defined as the supernatant from washed, thrombin-activated, allogenic, platelet-rich plasma cells obtained after centrifugation. Four animals were sacrificed at 4 weeks, and the remaining 4 were sacrificed at 8 weeks. For histomorphometric analyses, undecalcified ground specimens were prepared and stained with the Levai-Laczko stain.


RESULTS
For the entire follow-up time, 55.30% BIC was found with PRGF versus 38.91% without PRGF (P = .0198). At 4 weeks, BIC was 44.20% with PRGF versus 29.62% without PRGF (P = .0632), and at 8 weeks there was 70.36% BIC with PRGF versus 48.20% without PRGF (P = .1221).


CONCLUSIONS
Growth factors and other molecules released upon activation of platelet-rich plasma cells can enhance implant anchorage in cortical bone. PRGF obtained from allogenic sources does not impair healing.


CONCLUSIONS
The results indicated that a single application of PRGF before implant placement can be sufficient to increase the percent BIC in minipig cortical bone.
Fuerst
Gabor
G
Department of Oral Surgery, School of Dentistry, University of Vienna, Austria. gabor.fuerst@univie.ac.at
Gruber
Reinhard
R
Tangl
Stefan
S
Sanroman
Fidel
F
Watzek
Georg
G
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D015921:Dental Implants; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005615:Freezing; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D006133:Growth Substances / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000473:pathology*; D016348:Osseointegration / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects; D017713:Platelet Transfusion; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology
D015921:Dental Implants; D006133:Growth Substances