Morphological evidence of the importance of epithelial tissue during mouse tongue development.
The morphogenesis of fungiform papillae occurs in a stereotyped pattern on the dorsal surface of the tongue in mice from embryonic day 12 (E12) to E17. The histological results and ultrastructural observations showed the development of specific structures in the epithelium into fungiform papillae. Prior to the morphological changes, the Bmp-4 and Shh transcripts are expressed in a restricted area on the dorsal surface. These results suggest that the development of fungiform papillae requires an epithelium and mesenchyme interaction during morphogenesis. In order to obtain direct evidence of the epithelium and mesenchyme interaction during tongue papillae morphogenesis, the formation of fungiform papillae was examined after a recombination assay. In order to confirm the epithelium and mesenchyme interactions during the early development of the mouse tongue, a recombination assay was conducted after the recombination assay at E12.5 and E13.5 for 2 days using an in vitro organ culture. From the recombination assay results, the E13.5 epithelial portion of the fungiform papillae could determine the position of the newly formed fungiform papillae with the epithelial signaling molecules. E13.5 was a critical stage for fungiform papillae morphogenesis. Fungiform papillae can be considered to be small epithelial appendages, which are formed via the epithelium and mesenchyme interactions.
Division in Anatomy & Developmental Biology, Department of Oral Biology, Oral Science Research Center, College of Dentistry, BK 21 Project for Medical Sciences, Yonsei University, Seoul, Korea.
Jae-Young
JY
Kim
Tomoyuki
T
Mochizuki
Keiichi
K
Akita
Han-Sung
HS
Jung
D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002450:Cell Communication; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning; D009024:Morphogenesis; D009924:Organ Culture Techniques; D011247:Pregnancy; D015398:Signal Transduction; D013650:Taste Buds / Q000196:embryology*; D014059:Tongue / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D015534:Trans-Activators / Q000378:metabolism
C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D015534:Trans-Activators