Microfluidic device for single-cell analysis.
We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.
Wheeler
Aaron R
AR
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Throndset
William R
WR
Whelan
Rebecca J
RJ
Leach
Andrew M
AM
Zare
Richard N
RN
Liao
Yish Hann
YH
Farrell
Kevin
K
Manger
Ian D
ID
Daridon
Antoine
A
D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002477:Cells / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D019169:Jurkat Cells; D008838:Microcomputers; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D010777:Photochemistry; D012828:Silicones; D020298:U937 Cells
D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012828:Silicones; C013830:baysilon; D002118:Calcium