G484S amino acid substitution in lanosterol 14-alpha demethylase (ERG11) is related to fluconazole resistance in a recurrent Cryptococcus neoformans clinical isolate.
Five sequential Cryptococcus neoformans isolates recovered from an AIDS patient with recurrent meningitis were analyzed. Four isolates were fluconazole susceptible, while the fifth isolate developed fluconazole resistance. Analysis of the 14-alpha lanosterol demethylase gene (ERG11) showed a point mutation in the resistant strain responsible for the amino acid substitution G484S.
Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Hospital J. A. Fernandez, Buenos Aires, Argentina. lrodero@anlis.gov.ar
Laura
L
Rodero
Emilia
E
Mellado
A Carolina
AC
Rodriguez
Angela
A
Salve
Liliana
L
Guelfand
Pedro
P
Cahn
Manuel
M
Cuenca-Estrella
Graciela
G
Davel
Juan L
JL
Rodriguez-Tudela
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000328:Adult; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D008297:Male; D016919:Meningitis, Cryptococcal / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D017354:Point Mutation / Q000235:genetics; D012008:Recurrence; D058886:Sterol 14-Demethylase
D000935:Antifungal Agents; C548418:CYP51A1 protein, human; D015725:Fluconazole; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase