Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus.
Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inactivated EBOV species Zaire. Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses. An antigen capture ELISA was established, which is specific for EBOV-Zaire and shows a sensitivity of approximately 10(3) plaque-forming units/ml. Since the ELISA is able to detect even SDS-inactivated EBOV in spiked human sera, it could complement the existing diagnostic tools in the field and in routine laboratories where high containment facilities are not available.
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. andreaslu@gmx.de
Andreas
A
Lucht
Roland
R
Grunow
Christian
C
Otterbein
Peggy
P
Möller
Heinz
H
Feldmann
Stephan
S
Becker
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D029043:Ebolavirus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology*; D038301:Virus Inactivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D013501:Surface-Active Agents; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus; D012967:Sodium Dodecyl Sulfate