Overwinter fasting and re-feeding in rainbow trout: plasma growth hormone and cortisol levels in relation to energy mobilisation.
This study investigated the roles of cortisol and growth hormone (GH) during a period of fasting in overwintering salmonid fish. Indices of carbohydrate (plasma glucose, liver glycogen), lipid (plasma free fatty acids (FFAs)) and protein metabolism (plasma protein, total plasma amino acids) were determined, together with plasma GH, cortisol and somatolactin (SL) levels at intervals in three groups of rainbow trout (continuously fed; fasted for 9 weeks then fed; fasted for 17 weeks). In fasted fish, a decline in body weight and condition factor was accompanied by reduced plasma glucose and hepatic glycogen and increased plasma FFA. No consistent elevation of plasma GH occurred until after 8 weeks of fasting when plasma GH levels increased ninefold. No changes were observed in plasma total protein and AA until between weeks 13 and 17 when both were reduced significantly. When previously fasted fish resumed feeding, plasma glucose and FFA, and hepatic glycogen levels rapidly returned to control values and weight gain resumed. No significant changes in plasma cortisol levels, related to feeding regime, were evident at any point during the study and there was no evidence that SL played an active role in the response to fasting. The results suggest that overwinter fasting may not represent a significant nutritional stressor to rainbow trout and that energy mobilisation during fasting may be achieved without the involvement of GH, cortisol or SL.
The NERC Centre for Ecology and Hydrology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK. tgp@ceh.ac.uk
T G
TG
Pottinger
M
M
Rand-Weaver
J P
JP
Sumpter
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004734:Energy Metabolism / Q000502:physiology*; D005215:Fasting / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D029941:Fish Proteins; D006023:Glycoproteins / Q000097:blood*; D013006:Growth Hormone / Q000097:blood*; D006854:Hydrocortisone / Q000097:blood*; D008112:Liver Glycogen / Q000032:analysis; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010907:Pituitary Hormones / Q000097:blood*
D000596:Amino Acids; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D029941:Fish Proteins; D006023:Glycoproteins; D008112:Liver Glycogen; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D013006:Growth Hormone; D006854:Hydrocortisone