Control of a double helix DNA assembly by use of cross-linked oligonucleotides.
Disulfide cross-linked oligonucleotides for connecting two DNA double helixes have been designed, synthesized, and characterized. Employing these cross-linked oligonucleotides, two double helixes can be arranged side by side, and the orientations can be controlled both in parallel and antiparallel ways by addition of a specific complementary DNA strand.
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Masayuki
M
Endo
Tetsuro
T
Majima
D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D004229:Dithiothreitol / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D004247:DNA; D004228:Dithionitrobenzoic Acid; D004229:Dithiothreitol