Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay.
The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was >80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
Machado
Eleuza Rodrigues
ER
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brasil.
Ueta
Marlene Tiduko
MT
de Fátima Gonçalves-Pires
Maria do Rosário
Mdo R
Alves de Oliveira
João Batista
JB
Faccioli
Lúcia Helena
LH
Costa-Cruz
Julia Maria
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D012377:Rodentia; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth