LC-NMR and LC-MS analysis of 2,3,10,11-oxygenated protoberberine metabolites in Corydalis cell cultures.
The metabolism of 2,3,10,11-oxygenated protoberberine alkaloids was studied in cell cultures of Corydalis species. Without prior isolation, the structures of the metabolites were determined by LC-MS and LC-NMR analyses. Tetrahydropseudocoptisine alpha-N-metho salt, pseudoprotopine, and pseudomuramine were identified for the first time, and preliminary evidence for metabolic pathways to the formation of these alkaloids were obtained.
Kobe Pharmaceutical University, 4-19-1 Motoyamakita, Higashinada-ku, Kobe 658-8558, Japan. k-iwasa@kobepharma-u.ac.jp
Kinuko
K
Iwasa
Ayako
A
Kuribayashi
Makiko
M
Sugiura
Masataka
M
Moriyasu
Dong-Ung
DU
Lee
Wolfgang
W
Wiegrebe
D001600:Berberine Alkaloids / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D031311:Corydalis / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010084:Oxidation-Reduction
D001600:Berberine Alkaloids; C009090:protoberberine