IgM and IgG autoantibodies from microscopic polyangiitis patients but not those with other small- and medium-sized vessel vasculitides recognize multiple endothelial cell antigens.
Using a quantitative immunoblotting technique on extracts of macrovascular and microvascular endothelial cells (EC), we analyzed serum IgM and IgG reactivities of patients with active disease fulfilling the ACR and Chapel Hill criteria for the diagnosis of polyarteritis nodosa (PAN) (n = 8), PAN related to hepatitis B virus (HBV) infection (HBV-PAN) (n = 5), Wegener's granulomatosis (n = 6), microscopic polyangiitis (MPA) (n = 18), Churg-Strauss syndrome (n = 8), and patients with chronic HBV infection without PAN (n = 5) and age- and gender-matched healthy individuals (n = 45). MPA patients' IgM bound to 200-, 105-, 80-, 65-, 45-, 35-, and 33-kDa major bands, whereas IgM from controls and other patients bound predominantly to the 65-kDa band in EC extracts. MPA patients' IgG reacted mainly with 105-, 70-, 55-, and 38-kDa protein bands, whereas IgG from controls and other patients did not. Our results provide evidence that IgM and to a lesser degree IgG from MPA patients specifically recognize multiple EC antigens.
UPRES EA 3408, Formation Associée Claude-Bernard, UFR-SMBH Léonard-de-Vinci, Bobigny, France.
Youri
Y
Chanseaud
Paloma
P
García de la Peña-Lefebvre
Philippe
P
Guilpain
Alfred
A
Mahr
Mathieu C
MC
Tamby
Michèle
M
Uzan
Loïc
L
Guillevin
Marie Christophe
MC
Boissier
Luc
L
Mouthon
D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D015267:Churg-Strauss Syndrome / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D014890:Granulomatosis with Polyangiitis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010488:Polyarteritis Nodosa / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M