Nuclear inheritance of erythromycin resistance in human cells: new class of mitochondrial protein synthesis mutants.
The characterization of two new erythromycin-resistant mutants of HeLa cells is described. The strains ERY2305 and ERY2309 both exhibited resistance to erythromycin in growth assays and cell-free mitochondrial protein synthesis assays. The erythromycin resistance phenotype could not be transferred by cybridization. The mutation appeared to be encoded in the nucleus and inherited as a recessive trait. These two mutants, therefore, represent a new class of erythromycin-resistant mutants in human cells that is distinct from the cytoplasmically inherited mutation in strain ERY2301 described previously.
Doersen
C J
CJ
Department of Microbiology, California College of Medicine, University of California, Irvine, California 92717, USA.
Stanbridge
E J
EJ
D002455:Cell Division; D002467:Cell Nucleus / Q000235:genetics*; D002469:Cell Separation; D002470:Cell Survival; D002474:Cell-Free System; D002877:Chromosomes, Human / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D009154:Mutation / Q000235:genetics*
D024101:Mitochondrial Proteins; D004917:Erythromycin