New properties of simian virus 40 large T antigen.
An 8,000-molecular-weight (8K) T antigen was found in all cells transformed by simian virus 40. The 8K T antigen was weakly labeled in vivo with [35S]methionine or 32Pi. A deletion in the human papovavirus BK genome, in the region coding for the carboxy-terminal end of the large T antigen, reduced the size of the 8K T antigen. The last 80 amino acids of the large T antigen include the sequence Asp-Asp-Asp-Asp unique to the activation peptide of trypsinogen. Large T antigen bound diisopropyl fluorophosphate and was retained by D-phenylalanine coupled to Sepharose beads, an affinity adsorbent that can retain chymotrypsin. The large T antigen and the recA protein of Escherichia coli, a known protease, have several properties in common as well as several similar sequences. Antibodies against large T antigen interacted with native recA protein.
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France.
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D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001739:BK Virus / Q000235:genetics; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006801:Humans; D007531:Isoflurophate / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D013539:Simian virus 40 / Q000502:physiology*
D000952:Antigens, Polyomavirus Transforming; D011480:Protease Inhibitors; D007531:Isoflurophate