Hydrolysis of animal manure lignocellulosics for reducing sugar production.
Converting animal manure into value-added products provides a potential alternative for treatment and disposal of such materials. Lignocellulosics are a major component of animal manure and represent an undeveloped bioresource. In this work, a process was developed for hydrolyzing manure lignocellulosics into fermentable sugars. When raw dairy manure was pre-treated with 3% sulfuric acid at 110 degrees C for 1 h, hemicellulose was completely degraded into mainly arabinose, galactose and xylose. The pretreated materials were then treated with cellulolytic enzymes, Celluclast-1.5L and Novozyme-188, to hydrolyze the cellulose. The optimal enzyme loadings were identified as 13 FPU cellulase/g substrate and 5 IU beta-glucosidase/g substrate. The optimal temperature and pH were determined to be 46 degrees C and 4.8, respectively. A substrate concentration of 50 g/l favored both glucose concentration (in hydrolysate) and glucose yield (based on per 100 g manure). It was also found that a reduced particle size of 590-mum resulted in a high glucose yield with further decreases in particle size not increasing the yield. For each particle size investigated, the addition of 2% tween-80 resulted in at least 20% improvement in glucose yield. The optimized hydrolysis process achieved a glucose yield of 11.32 g/100 g manure, which corresponded to about 40% cellulose conversion.
Wen
Zhiyou
Z
Department of Biological Systems Engineering, Center of Multiphase Environmental Research, Washington State University, Pullman, WA 99164, USA. zwen@wsu.edu
Liao
Wei
W
Chen
Shulin
S
D002241:Carbohydrates / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D008372:Manure; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry; D013464:Sulfuric Acids; D013501:Surface-Active Agents; D013696:Temperature
D002241:Carbohydrates; D004798:Enzymes; D008372:Manure; D011134:Polysaccharides; D013464:Sulfuric Acids; D013501:Surface-Active Agents; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; C033158:sulfuric acid