Characterization of the major allergens purified from the venom of the paper wasp Polistes gallicus.
Allergic reactions to vespid stings are one of the major causes of IgE-mediated anaphylaxis. Vespa and Vespula venoms are closely related; Polistes venom is more distantly related and its allergens are less well studied. There is limited cross-reactivity between Polistes and the other vespid venoms because of differences in the epitopes on the allergen molecules. In this study, the major allergens of Polistes gallicus are isolated and characterized. P. gallicus venom contains four major allergens: phospholipase, antigen 5 (Ag5), hyaluronidase and protease that were characterized by mass spectrometry and specific binding to IgE. The complete amino acid sequence of Ag5 and the sequence of the N-terminal region of phospholipase were also determined. The alignment of Ag5 from P. gallicus (European species) and Polistes annularis (American species) shows an 85% identity that increases to 98% within the same subgenus. This could suggest the presence of specific epitopes on Ag5 molecule being the variations on the superficial loops. The features of the P. gallicus allergens could explain the partial cross-reactivity found between the American and European Polistes venoms, and suggest that the use of European Polistes venoms would improve the diagnostic specificity and the therapy of European patients and of North American patients sensitized by European Polistes.
Department of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy. yama@med.teikyo-u.ac.jp
Barbara
B
Pantera
Donald R
DR
Hoffman
Lara
L
Carresi
Gianni
G
Cappugi
Stefano
S
Turillazzi
Giampaolo
G
Manao
Maurizio
M
Severino
Igino
I
Spadolini
Giuseppe
G
Orsomando
Gloriano
G
Moneti
Luigia
L
Pazzagli
D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000209:etiology; D000818:Animals; D003429:Cross Reactions; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000209:etiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D013045:Species Specificity; D014862:Wasp Venoms / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014863:Wasps / Q000235:genetics / Q000276:immunology
D000485:Allergens; D014862:Wasp Venoms