A comparative study of motor-protein motions by using a simple elastic-network model.
In this work, we report on a study of the structure-function relationships for three families of motor proteins, including kinesins, myosins, and F1-ATPases, by using a version of the simple elastic-network model of large-scale protein motions originally proposed by Tirion [Tirion, M. (1996) Phys. Rev. Lett. 77, 1905-1908]. We find a surprising dichotomy between kinesins and the other motor proteins (myosins and F1-ATPase). For the latter, there exist one or two dominant lowest-frequency modes (one for myosin, two for F1-ATPase) obtained from normal-mode analysis of the elastic-network model, which overlap remarkably well with the measured conformational changes derived from pairs of solved crystal structures in different states. Furthermore, we find that the computed global conformational changes induced by the measured deformation of the nucleotide-binding pocket also overlap well with the measured conformational changes, which is consistent with the "nucleotide-binding-induced power-stroke" scenario. In contrast, for kinesins, this simplicity breaks down. Multiple modes are needed to generate the measured conformational changes, and the computed displacements induced by deforming the nucleotide-binding pocket also overlap poorly with the measured conformational changes, and are insufficient to explain the large-scale motion of the relay helix and the linker region. This finding may suggest the presence of two different mechanisms for myosins and kinesins, despite their strong evolutionary ties and structural similarities.
Department of Physics and Applied Physics, Stanford University, Stanford CA 94305, USA.
Wenjun
W
Zheng
Sebastian
S
Doniach
D000255:Adenosine Triphosphate / Q000737:chemistry; D055592:Biophysical Phenomena; D001703:Biophysics; D018360:Crystallography, X-Ray; D016547:Kinesins / Q000737:chemistry; D015233:Models, Statistical; D008962:Models, Theoretical; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009218:Myosins / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000737:chemistry; D013329:Structure-Activity Relationship
D020409:Molecular Motor Proteins; D009711:Nucleotides; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D009218:Myosins; D016547:Kinesins