Determination of the compositions of polysaccharides from Chinese herbs by capillary zone electrophoresis with amperometric detection.
In this paper, capillary zone electrophoresis with amperometric detection (CZE-AD) was applied to determine the compositions of hetero-polysaccharides from Chinese herbs, Angelica sinensis and flax by analyzing their hydrolyzed monosaccharides: fucose, galactose, glucose, arabinose, rhamnose and xylose. Under the selected optimum conditions, the six monosaccharides could be perfectly separated within 25 min and showed significant current responses at copper electrodes. The linear ranges of the six monosaccharides were all from 5.0 x 10(-6) to 2.0 x 10(-4) mol L(-1) and their detection limits were lower or near 1.0 x 10(-6) mol L(-1) (S/N = 3). Experiments showed that the Angelica sinensis polysaccharide was composed of fucose, galactose, glucose, arabinose, rhamnose and xylose (mole ratio 1.0:13.6:15.0:8.7:21.3:3.7), and the flax polysaccharide was composed of galactose, glucose and arabinose (mole ratio 1.0:4.98:1.1). The purity of these polysaccharides leached by the introduced leaching method was 98.3 and 97.6%, respectively. Analyzing polysaccharides by this method has some merits of speed, simple instrumentation and operation, high sensitivity and high reproducibility.
Wang
Qingjiang
Q
Department of Chemistry, East China Normal University, Shanghai 200062, People's Republic of China.
Ding
Fei
F
Zhu
Ningning
N
He
Pingang
P
Fang
Yuzhi
Y
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides