In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus.
Cellular tropism and transcription of bovine leukemia virus (BLV) and bovine immunodeficiency-like virus (BIV) were investigated using peripheral blood mononuclear cells (PBMC) collected from a cow infected with both viruses. Each PBMC subset, purified by magnetic cell sorting, was subjected to PCR and RT-PCR for detection of their integrated proviruses and transcript mRNAs. Both BLV and BIV genomes were detected by nested PCR in CD3(+), CD4(+), CD8(+) and gammadelta T cells, B cells and monocytes. However, BLV tax transcription was only detected in B cells, and only B cells also formed BLV syncytia in CC81 cells. On the other hand, BIV transcript was detected in each subpopulation of PBMC. These results indicated that BLV can infect T cells and monocytes as well as B cells, but can be expressed by transcription only in B cells. In contrast, BIV can express its transcripts in all infected cells.
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Donglai
D
Wu
Kenji
K
Murakami
Akira
A
Morooka
Hong
H
Jin
Yasuo
Y
Inoshima
Hiroshi
H
Sentsui
D000818:Animals; D001402:B-Lymphocytes / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D016583:Enzootic Bovine Leukosis / Q000821:virology*; D015726:Giant Cells; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009000:Monocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000821:virology; D014158:Transcription, Genetic