Karyotyping of human synaptonemal complexes by cenM-FISH.
The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cel.lular, Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain. moliver@servet.uab.es
Maria
M
Oliver-Bonet
Thomas
T
Liehr
Angela
A
Nietzel
Anita
A
Heller
Heike
H
Starke
Uwe
U
Claussen
Montserrat
M
Codina-Pascual
Aïda
A
Pujol
Carlos
C
Abad
Josep
J
Egozcue
Joaquima
J
Navarro
Jordi
J
Benet
D002503:Centromere / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007246:Infertility; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000235:genetics*