Improved spiral disk assembly for high-speed counter-current chromatography.
The spiral disk for type-J high-speed counter-current chromatography (HSCCC) has been improved by placing short segments of PTFE (tetrafluoroethylene) tubing into the channel at regular intervals. The best results were obtained with a four-channel spiral column using 600 spacers, which significantly improved stationary phase retention and partition efficiency in both n-butanol-acetic acid-water (4:1:5) and poly(ethylene glycol) 1000-dibasic phosphate polymer phase systems. Based on these findings, a "bead-chain spiral disk" was designed and its performance tested with a type-J HSCCC centrifuge. This new column design substantially improved the separation of both dipeptide and protein samples in terms of stationary phase retention and partition efficiency.
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 3334, Bethesda, MD 20892-8014, USA. itoy@nhlbi.nih.gov
Yoichiro
Y
Ito
Fuquan
F
Yang
Paul
P
Fitze
Jimmie
J
Powell
David
D
Ide
D003377:Countercurrent Distribution / Q000295:instrumentation*; D004867:Equipment Design