Simple PbII fluorescent probe based on PbII-catalyzed hydrolysis of phosphodiester.
A new fluorescent probe for PbII, p-nitrophenyl 3H-phenoxazin-3-one-7-yl phosphoric acid (NPPA), was designed and synthesized by linking resorufin (serving as a fluorophore and electron acceptor) to p-nitrophenol (serving as a fluorescence quencher and electron donor) through phosphodiester bonds. When NPPA was irradiated with light, intramolecular fluorescence self-quenching took place because of the photoinduced electron transfer from the donor to the acceptor. However, upon the addition of PbII, the phosphate ester bonds in the probe were cleaved and the fluorophore was released, accompanying the retrievement of fluorescence.
Laboratory of Chemical Biology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Ming
M
Sun
Dihua
D
Shangguan
Huimin
H
Ma
Lihua
L
Nie
Xiaohua
X
Li
Shaoxiang
S
Xiong
Guoquan
G
Liu
Wolfram
W
Thiemann
D002384:Catalysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007854:Lead / Q000737:chemistry*; D015394:Molecular Structure; D010755:Organophosphates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005456:Fluorescent Dyes; D010755:Organophosphates; D007854:Lead