Generation of antibodies against prion protein in wild-type mice via helix 1 peptide immunization.
We present here the development of antibodies against prion protein in BALB/C mice using as antigen human helix 1 of PrP. This sequence is suggested to be involved in protein pathological conformational changes, and is distinguished from that of mice by one amino acid. The immune tolerance to an 'almost-self' epitope and the poor immunogenicity of short peptides was overcome by using Multiple Antigen Peptide displaying eight copies of helix 1. The generated antibodies recognize the whole prion protein with a high binding constant and the established protocol may lead to an active immunization towards therapeutics of prion disease.
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
Michal
M
Arbel
Vered
V
Lavie
Beka
B
Solomon
D000818:Animals; D000910:Antibodies, Heterophile / Q000096:biosynthesis* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015478:Antigens, Heterophile / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D002417:Cattle; D045744:Cell Line, Tumor; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000276:immunology* / Q000378:metabolism; D018621:PrPC Proteins / Q000276:immunology* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011817:Rabbits; D012756:Sheep; D014611:Vaccination / Q000379:methods
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D018621:PrPC Proteins