Peptidoglycan recognition protein expression in mouse Peyer's Patch follicle associated epithelium suggests functional specialization.
Mammalian Peyer's Patches possess specialized epithelium, the follicle associated epithelium (FAE), and specialized cells called M cells which mediate transcytosis of antigens to underlying lymphoid tissue. To identify FAE specific genes, we used TOGA gene expression profiling of microdissected mouse Peyer's Patch tissue. We found expression of laminin beta3 across the FAE, and scattered expression of peptidoglycan recognition protein (PGRP)-S. Using the M cell specific lectin Ulex europaeus agglutinin 1 (UEA-1), PGRP-S expression was nearly exclusively co-localized with UEA-1+ M cells. By contrast, the related gene PGRP-L was expressed among a subset of UEA-1 negative FAE cells. Expression of these proteins in transfected cells demonstrated distinct subcellular localization. PGRP-S showed a vesicular pattern and extracellular secretion, while PGRP-L showed localization to both the cytoplasm and the cell surface. The potential function of these PGRP proteins as pattern recognition receptors and their distinctive cellular distribution suggests a complex coordination among specialized cells of the FAE in triggering mucosal immunity and innate immune responses.
Digital Gene Technologies, Inc., La Jolla, CA, USA. davidlo@dgt.com
David
D
Lo
Wendy
W
Tynan
Janet
J
Dickerson
Jason
J
Mendy
Hwai-Wen
HW
Chang
Melinda
M
Scharf
Daragh
D
Byrne
David
D
Brayden
Lisa
L
Higgins
Claire
C
Evans
Daniel J
DJ
O'Mahony
D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002451:Cell Compartmentation / Q000276:immunology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D020869:Gene Expression Profiling; D007113:Immunity, Innate / Q000235:genetics; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007797:Laminin / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D037121:Plant Lectins / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D007797:Laminin; D037121:Plant Lectins; D012333:RNA, Messenger; C036850:Ulex europaeus lectins; C118897:peptidoglycan recognition protein