Metabolic characteristics of an isocitrate dehydrogenase defective derivative of escherichia coli BL21(DE3).
A 7.8 kb fragment containing isocitrate dehydrogenase (ICDH) gene and its flanking regions was cloned from Escherichia coli BL21(DE3) and sequenced. Unlike the case of the K-12 strain, the e14 element was not found. The nucleotide divergence between these two strains was about 2%. Using the cloned fragment, ICDH defective mutant strain, MA1935, was generated from BL21(DE3). Although MA1935 accumulated citrate, citrate synthase activity was not repressed but was rather high. In addition, isocitrate lyase was not highly induced at the stationary phase. MA1935 was shown to be a good host strain for ICDH gene expression.
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. aomiho@mail.ecc.u-tokyo.ac.jp
Miho
M
Aoshima
Masaharu
M
Ishii
Akihiko
A
Yamagishi
Tairo
T
Oshima
Yasuo
Y
Igarashi
D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D015202:Protein Engineering / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology
D007521:Isocitrate Dehydrogenase