Construction of gene expression system in hop (Humulus lupulus) lupulin gland using valerophenone synthase promoter.
The promoter region of the valerophenone synthase (VPS) gene was isolated from hop (Humulus lupulus). VPS, a member of the chalcone synthase (CHS) super-family, catalyzes the biosynthesis reaction of the hop resin that significantly accumulates in the cone's secretory gland called the "lupulin gland". The typical H-box and G-box sequences, which exist in many plants' CHS promoters and act as cis-elements for tissue specificity, UV-light induction, etc., were not found in the isolated VPS promoter, although the H-box-like sequence (CCTTACC, CCTAACC) and the core sequence (ACGT) of the G-box were observed. The transformation experiment using the VPS promoter-UIDA gene fusion revealed that the promoter acts not only in the lupulin gland but also in the glands of leaf and stem. On the other hand, the VPS promoter activity was not induced by UV-irradiation.
Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd., 37-1, Kizaki, Nitta, Gunma 370-0393, Japan. yukio.okada@sapporobeer.co.jp
Yukio
Y
Okada
Kazuo
K
Saeki
Akira
A
Inaba
Narushi
N
Suda
Takafumi
T
Kaneko
Kazutoshi
K
Ito
D000217:Acyltransferases / Q000235:genetics*; D019677:Artificial Gene Fusion; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression / Q000528:radiation effects; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D027582:Humulus / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D018514:Plant Structures / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014466:Ultraviolet Rays
D018744:DNA, Plant; D000217:Acyltransferases; C432588:isovaleryl-CoA malonyl-CoA acyltransferase; D005966:Glucuronidase