[Induction of biochemical composition in Heterosigma akashiwo under Fe stress].
The iron-stress mediated effects on biochemical constituents of red tide alga H. akashiwo were examined in Fe-replete and iron deficiency, and low iron batch cultures. The content of all pigments decreased under iron limitation, and the cellular chlorophyll a concentration decreased by more than 2-fold compared to that under iron replete condition. This change in Chl a was accompanied by a corresponding decrease in Chl c per cell, resulting in no trend in the ratio of Chl c to Chl a. The cellular carotenoids content decreased by more than 1.5-fold compared to that under iron replete condition. As a consequence, carotenoids/chlorophyll a ratio increased in Fe-deficient cells. Carbohydrate content was reduced under iron stress, and total proteins decreased with the decrease of iron concentration. A crude fractionation of the soluble proteins demonstrated that 17 kD and 55 kDa proteins in soluble fraction were induced.
Li
Dongxia
D
National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China. lidongxia1974@yahoo.com
Cong
Wei
W
Cai
Zhaoling
Z
Shi
Dingji
D
Ouyang
Fan
F
D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis*; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism; D057097:Harmful Algal Bloom; D007501:Iron / Q000378:metabolism*; D010860:Pigments, Biological / Q000032:analysis*
D010860:Pigments, Biological; D002734:Chlorophyll; D002338:Carotenoids; D007501:Iron