Detection of staphylococcal enterotoxin B in spiked food samples.
Contamination of food with infectious agents, intentional or not, is a global concern with far-reaching economic and social impact. Staphylococcal enterotoxins are a major cause of food poisoning, but most methods for the identification of these agents in food require extensive pretreatment or concentration of the sample prior to analysis. The array biosensor was developed as a portable device for the simultaneous analysis of multiple complex samples for multiple targets with minimal sample preparation. In this study, we use an array biosensor to expand and improve on a staphylococcal enterotoxin B (SEB) assay with the ultimate intent of incorporating testing for SEB into a battery of sensitive and convenient assays for food safety validation. In addition to buffer studies, six different types of food samples, including beverages, homogenates of fruit and meat, and carcass washings, were spiked with SEB, incubated for at least 2 h to permit antigen sequestration, and assayed. For all samples, there were differences in fluorescence intensity, but 0.5 ng of SEB per ml could be detected in <20 min with little if any pretreatment and no sample preconcentration.
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375-5348, USA. lcs@cbmse.nrl.navy.mil
Lisa C
LC
Shriver-Lake
Yura S
YS
Shubin
Frances S
FS
Ligler
D015374:Biosensing Techniques / Q000379:methods*; D003257:Consumer Product Safety; D004768:Enterotoxins / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus; D013997:Time Factors
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal