[Occurrence and characteristic of P-like adhesin among Klebsiella strains].
Colonisation and remaining of microorganism on mucus membrane of microorganism is tightly connected with adhesion mechanisms and determine the first step of physiological settlement of the organism or the first stage of clinically demonstrated infection. In Klebsiella rods there are known three types of fimbrial adhesins (type 1, 3 and KPF-28) and non-fimbrial adhesin CF29K. It is stated that Klebsiella strains adhesions are responsible for their adherence to the epithelial cells of both respiratory and urinary tracts and to intestine epithelium. The in vitro research affirmed Klebsiella rods adherence to protein matrix. The aim of our work was the establishment of character, receptor specificity and the appearance frequency of P-like called adhesin. The frequency of expression of P-like adhesin was estimated among 380 isolated from the patients strains on the basis of agglutinating methods. The amorphic character of P-like adhesin was proved using electron microscopy method. The isolation and purification of P-like protein with a help of affinity chromatography enabled to estimate the receptor specificity of the adhesin. The receptor specificity was established as similar to E.coli PapG adhesin.
Przondo-Mordarska
Anna
A
Katedra i Zakład Mikrobiologii Akademii Medycznej we Wrocławiu.
Smutnicka
Danuta
D
Matusiewicz
Katarzyna
K
D018829:Adhesins, Bacterial / Q000302:isolation & purification / Q000502:physiology*; D001422:Bacterial Adhesion; D006801:Humans; D066298:In Vitro Techniques; D007709:Klebsiella / Q000302:isolation & purification / Q000472:pathogenicity*; D008854:Microscopy, Electron; D013045:Species Specificity
D018829:Adhesins, Bacterial