Immunoblot analysis of Trichomonas vaginalis antigens recognized by rabbit hyperimmune serum raised against exoantignes.
The SDS-PAGE and immunoblot were used to identify Trichomonas vaginalis antigenic epitopes present in purified somatic and exo-antigens. The presence of common immunogenic proteins corresponding to molecular weights of 76, 60 and 23 kDa was revealed. Distinctive immunogenic bands of 92, 72, 55 and 40 kDa for the exo-antigens, and of 110, 80, 78 and 50 kDa for the somatic antigens, appeared when the antigens were probed by the homologous immune rabbit serum.
Khalifa
Khalifa E
KE
Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., 11566, Cairo, Abbassia, Egypt.
El-Wakil
Hala S I
HS
Habib
Fayza S
FS
Abdel Baki
Mohamed H
MH
Azab
Magda E
ME
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D008875:Middle Aged; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000276:immunology; D014246:Trichomonas vaginalis / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D015800:Protozoan Proteins