Disruption of sabR affects nikkomycin biosynthesis and morphogenesis in Streptomyces ansochromogenes.
The gene, sabR, encoding a receptor for gamma-butyrolactone, was cloned from the genomic DNA of Streptomyces ansochromogenes 7100. Its deduced protein shows strong homology to several gamma-butyrolactone-binding proteins in Streptomyces. Disruption of sabR retarded nikkomycin production in liquid media containing glucose or glycerol as carbon source. Sporulation of sabR disruption mutants was earlier than the parent strain on solid media with glucose or glycerol as carbon source. However, disruption of sabR had no effect on either nikkomycin production or sporulation on media containing mannitol as carbon source, suggesting that sabR is a pleiotropic regulatory gene that controls the onset of nikkomycin production and sporulation in S. ansochromogenes and is related to the utilization of carbon source.
Department of Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Wenli
W
Li
Gang
G
Liu
Huarong
H
Tan
D015107:4-Butyrolactone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011963:Receptors, GABA-A / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
C037481:4-butyrolactone receptor; D000617:Aminoglycosides; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D011963:Receptors, GABA-A; D011994:Recombinant Proteins; D012097:Repressor Proteins; C011952:nikkomycin; D015107:4-Butyrolactone