High-throughput screening for gene libraries expressing carbohydrate hydrolase activity.
A simple and fast method is described allowing screening of large number of Escherichia coli clones (4000 per day) for the presence of functional or improved carbohydrate hydrolase enzymes. The procedure is relatively cheap and has the advantage that carbohydrate degrading activity can be directly measured using liquid cultures grown in microtiter plates without the need of separation or purification steps.
Leemhuis
Hans
H
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Euverink
Gert-Jan W
GJ
Dijkhuizen
Lubbert
L
D050260:Carbohydrate Metabolism; D003001:Cloning, Molecular; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism; D009154:Mutation
D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D006867:Hydrolases