Encapsulation of Ole e 1 in biodegradable microparticles induces Th1 response in mice: a potential vaccine for allergy.
Ole e 1 is the most prevalent allergen of olive (Olea europaea) pollen. The suitability of poly(D,L-lactide-co-glycolide) (PLG) microparticles as a vaccine delivery system for Ole e 1 in therapy against olive allergy has been evaluated. BALB/c mice were immunized intraperitoneally with Ole e 1 in PLG microparticles. Encapsulated Ole e 1 induced specific and long-lasting antibodies that were predominantly of the IgG2a isotype. Splenic cells from immunized mice secreted in vitro IFN-gamma, but not IL-4, after stimulation with Ole e 1. These results suggest that Ole e 1-containing PLG microparticles elicit a specific Th1-type immune response and may become a promising concept for allergy vaccine.
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain. ameva@bbm1.ucm.es
Eva
E
Batanero
Patricia
P
Barral
Mayte
M
Villalba
Rosalía
R
Rodríguez
D000485:Allergens / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000536:Aluminum Hydroxide / Q000737:chemistry; D000818:Animals; D052179:Antigens, Plant; D004339:Drug Compounding / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006967:Hypersensitivity / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010940:Plant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D018417:Th1 Cells / Q000276:immunology*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000485:Allergens; D052179:Antigens, Plant; D007074:Immunoglobulin G; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; D011108:Polymers; D014612:Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D015847:Interleukin-4; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide; D007371:Interferon-gamma