Protein size distribution and inhibitory effect of wheat hydrolysates on Neutrase.
Neutrase, used for hydrolysis of wheat proteins, was inhibited by end-product in a competitive uncompetitive way. The inhibition ratio depends on the progress of protein hydrolysis (degree of hydrolysis) and it remains constant beyond a degree of hydrolysis of 7.5%. The inhibitor was separated, on Sephadex G-25 column, in three fractions (>2.4, 2.4-0.5 and <0.5 kDa) generating an inhibition ratio of 29%, 46% and 67% respectively. The peptides size distribution (<1, 1-2, 2-3 and >3 kDa) of fractions was determined using size exclusion-high performance liquid chromatography. The analysis of obtained data, using a simple mathematical regression, showed a correlation factor of 0.98 between the inhibition ratio and the peptides less than 1 kDa and 0.99 when considering the peptides lower than 1 kDa and higher than 3 kDa.
Laboratoire de Microbiologie Industrielle, Centre de Biotechnologie de Sfax, BP K, 3038, Sfax, Tunisia. radhouan.kammoun@cbs.rnrt.tn
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Bejar
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Ellouz
D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D012044:Regression Analysis; D014908:Triticum / Q000737:chemistry*
D010940:Plant Proteins; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases