Monitoring base excision repair by in vitro assays.
Base excision repair (BER) is the major mechanism for processing of mutagenic and genotoxic DNA base lesions caused by cellular environmental or exogenous mutagens. In vitro base excision repair assays have been used in studies aimed at understanding the nature of various human diseases, aging and mechanisms of genome recovery after mutagenic stress. This review describes the key base excision repair assays and addresses their advantages and limitations.
MRC Radiation and Genome Stability Unit, Medical Research Council, Harwell, OX11 0RD Oxfordshire, UK. g.dianov@har.mrc.ac.uk
Grigory L
GL
Dianov
D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D004260:DNA Repair / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data
D004270:DNA, Circular