Immune response in mice inoculated with plasmid DNAs containing multiple-epitopes of foot-and-mouth disease virus.
This paper focuses on the development of candidate DNA vaccine encoding antigenic epitopes of type O foot-and-mouth disease virus (FMDV). A series of plasmids encoding different combinations of B cell epitopes and a T cell epitope were constructed and characterized by inoculating BALB/c mice. The specific antibodies were only detectable in the mice inoculated with plasmids encoding the T cell epitope and B cell epitopes from sites 5 and 1, within which site 5 includes residues 135-167 of VP1 and site 1 includes 141-160 region (G-H loop) and carboxyl terminus of VP1. Stronger cellular immune responses were also observed in these mice using T cell proliferation assay.
Zhang
Hong-Ying
HY
Department of Medical Genetics, Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, China.
Sun
Shu-Han
SH
Guo
Ying-Jun
YJ
Zhou
Fen-Juan
FJ
Chen
Zu-Huan
ZH
Lin
Yi
Y
Shi
Ke
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D018985:Epitopes, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000235:genetics* / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D015151:Immunoblotting; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D019444:Vaccines, DNA; D014765:Viral Vaccines