Hydrogen bonds lengths in nucleic acids estimated from longitudinal nitrogen-15 relaxation.
A new NMR method has been designed for the measurement of the longitudinal relaxation rates of both donor and acceptor nitrogen-15 nuclei in Watson-Crick base pairs in 15N-enriched nucleic acids. The experiment was applied to a 22-nucleotide RNA hairpin. The lengths of four hydrogen bonds could be estimated from the longitudinal relaxation rates.
Bytchenkoff
Dimitri
D
Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland.
Bodenhausen
Geoffrey
G
D000465:Algorithms; D020029:Base Pairing; D003461:Crystallography / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry; D011119:Polynucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
D009587:Nitrogen Isotopes; D009696:Nucleic Acids; D011119:Polynucleotides; D012313:RNA; D006859:Hydrogen