Role of mitogen activated protein kinase cascade pathway and anti-stress response in liver transplantation.
OBJECTIVE
To explore the changes of mitogen activated protein kinase (MAPK) cascade pathway and anti-stress response of hepatocytes after liver transplantation.


METHODS
Ten normal liver specimens and 18 punctured donor liver specimens were divided into 3 groups: A (control:10), B (no rejection:10) and C (acute rejection:8). MAPK, Ras, Jun and heat shock protein 70 (HSP70) were tested immunohistochemically while Ras and HSP70 were tested by in situ hybridization. All sections were subjected to image analysis.


RESULTS
Protein expressions of MAPK, Ras and Jun were increased by an ascending order of groups A, B and C. The protein expression of HSP70 was the highest in group B but lower in group C. Expressions of Ras and HSP70 mRNA were consistent with those of protein.


CONCLUSIONS
The changes of the MAPK cascade pathway and anti-stress response of hepatocytes after liver transplantation are one of regulation mechanisms for protecting the hepatocytes from damage after liver transplantation. This mechanism is active to support individual survival
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. nqgong@tjh.tjmu.edu.cn
Nian-Qiao
NQ
Gong
Jian-Sheng
JS
Xiao
Jin-Sheng
JS
Zeng
Guo-Xun
GX
Li
Hui
H
Guo
Wei-Jie
WJ
Zhang
Qi-Fa
QF
Ye
D015870:Gene Expression; D006084:Graft Rejection / Q000503:physiopathology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D016031:Liver Transplantation; D020935:MAP Kinase Signaling System / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D013312:Stress, Physiological / Q000503:physiopathology*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D016755:Proto-Oncogene Proteins c-jun; D018631:ras Proteins