Accumulation of peribronchial mast cells in a mouse model of ovalbumin allergen induced chronic airway inflammation: modulation by immunostimulatory DNA sequences.
Few peribronchial mast cells are noted either in the lungs of naive mice or in the lungs of OVA-sensitized mice challenged acutely with OVA by inhalation. In this study, we demonstrate that OVA-sensitized mice exposed to repetitive OVA inhalation for 1-6 mo have a significant accumulation of peribronchial mast cells. This accumulation of peribronchial mast cells is associated with increased expression of the Th2 cell-derived mast cell growth factors, including IL-4 and IL-9, but not with the non-Th2 cell-derived mast cell growth factor, stem cell factor. Pretreating mice with immunostimulatory sequences (ISS) of DNA containing a CpG motif significantly inhibited the accumulation of peribronchial mast cells and the expression of IL-4 and IL-9. To determine whether mast cells express Toll-like receptor-9 (TLR-9; the receptor for ISS), TLR-9 expression by mouse bone marrow-derived mast cells (MBMMCs) was assessed by RT-PCR. MBMMCs strongly expressed TLR-9 and bound rhodamine-labeled ISS. However, incubation of MBMMCs with ISS in vitro neither inhibited MBMMC proliferation nor inhibited Ag/IgE-mediated MBMMC degranulation, but they did induce IL-6. Overall these studies demonstrate that mice exposed to repetitive OVA challenge, but not acute OVA challenge, have an accumulation of peribronchial mast cells and express increased levels of mast cell growth factors in the lung. Although mast cells express TLR-9, ISS does not directly inhibit mast cell proliferation in vitro, suggesting that ISS inhibits accumulation of peribronchial mast cells in vivo by indirect mechanism(s), which include inhibiting the lung expression of Th2 cell-derived mast cell growth factors.
Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Reid K
RK
Ikeda
Marina
M
Miller
Jyothi
J
Nayar
Linda
L
Walker
Jae Youn
JY
Cho
Kirsti
K
McElwain
Shauna
S
McElwain
Eyal
E
Raz
David H
DH
Broide
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001980:Bronchi / Q000276:immunology / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D001985:Bronchial Provocation Tests; D002449:Cell Aggregation / Q000276:immunology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002908:Chronic Disease; D018899:CpG Islands / Q000276:immunology*; D004247:DNA / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use; D007073:Immunoglobulin E / Q000502:physiology; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D007279:Injections, Subcutaneous; D015847:Interleukin-4 / Q000096:biosynthesis; D016906:Interleukin-9 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D016210:Methacholine Chloride / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D012235:Rhodamines / Q000378:metabolism; D051217:Toll-Like Receptor 9
D000276:Adjuvants, Immunologic; D000485:Allergens; C408982:CPG-oligonucleotide; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D006131:Growth Inhibitors; D016906:Interleukin-9; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface; D012235:Rhodamines; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D016210:Methacholine Chloride; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin; D004247:DNA