Human cord blood-derived mesenchymal stem cells home and survive in the marrow of immunodeficient mice after systemic infusion.
Bone marrow is the residence site of mesenchymal stem cells (MSC), which upon commitment and maturation develop into several mesenchymal phenotypes. Recently, we have described the presence of MSC in human cord blood (cbMSC) and informed that their properties are the same as those for MSC obtained from adult bone marrow. In this study we have investigated the capability of transplanted cbMSC to home and survive in the marrow of unconditioned nude mice. cbMSC utilized for transplantation studies were characterized by morphology, differentiation potential, and immunophenotype. After transplantation by systemic infusion, human DNA (as detected by PCR amplification of human-specific beta-globin gene) was detected in the marrow of recipients as well as in ex vivo-expanded stromal cells prepared from the marrow of transplanted animals. These results demonstrate homing and survival of cbMSC into the recipient marrow and also suggest a mesenchymal-orientated fate of engrafted cells, because human DNA was also detected in cells of other recipient tissues, like cardiac muscle, teeth, and spleen.
Erices
Alejandro A
AA
Programa Terapias Génicas y Celulares, INTA, Universidad de Chile, Santiago, Chile. aerices@uec.inta.uchile.cl
Allers
Carolina I
CI
Conget
Paulette A
PA
Rojas
Cecilia V
CV
Minguell
José J
JJ
D000818:Animals; D001853:Bone Marrow / Q000276:immunology / Q000601:surgery*; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D048429:Cell Size / Q000276:immunology; D002633:Chemotaxis / Q000276:immunology; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods* / Q000639:trends; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005914:Globins / Q000235:genetics; D006085:Graft Survival / Q000276:immunology*; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology / Q000628:therapy*; D016130:Immunophenotyping; D007231:Infant, Newborn; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods* / Q000639:trends; D051379:Mice; D008819:Mice, Nude; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology; D014183:Transplantation, Heterologous
D005914:Globins; D004247:DNA