An immunoassay for atrazine using tunable immunosorbent.
A novel, simple, economical, and environmentally friendly tunable immunosorbent-based immunoassay for sensitive and selective determination of atrazine is reported. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single-chain Fv of an anti-atrazine antibody were synthesized biologically and purified by temperature-triggered phase transition. A competitive immunoassay based on the competition of atrazine-horseradish peroxidase and atrazine was established with IC(50) and lower detection limit of 0.16 and 0.01 ppb, respectively. Excellent recoveries (mean values ranging between 92 and 104%) were demonstrated in simulated atrazine-contaminated water samples.
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
Jae-Young
JY
Kim
Ashok
A
Mulchandani
Wilfred
W
Chen
D000906:Antibodies / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001280:Atrazine / Q000032:analysis* / Q000276:immunology; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism; D007164:Immunosorbents / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis
D000906:Antibodies; D007135:Immunoglobulin Variable Region; D007164:Immunosorbents; D011993:Recombinant Fusion Proteins; D014874:Water Pollutants, Chemical; D004549:Elastin; D006735:Horseradish Peroxidase; D001280:Atrazine