[Real-time PCR for detection of molecular markers of resistance in Plasmodium falciparum].
Plasmodium falciparum drug resistance is a major problem in malaria endemic areas. Molecular markers and in vitro tests have been developed to study and monitor drug resistance. However, none used alone, can provide sufficient data concerning the level of drug resistance and to issue precise guideline for drug use policies in endemic areas. We propose real-time PCR for the simultaneous detection of pfcrt and pfmdr1 genes mutations. The aim of this study was not to provide definitive data concerning the rate of mutations in an endemic area, but to describe a powerful method allowing the detection of major pfmdr1 and pfcrt mutations.
de Monbrison
F
F
Laboratoire de parasitologie, mycologie médicale et pathologie exotique, E.A. 3087, faculté de médecine, université Claude-Bernard, 8, avenue Rockefeller, 69373 Lyon cedex 03, France. fdmonb@rockefeller.uni-lyon1.fr
Raynaud
D
D
Latour-Fondanaiche
C
C
Angei
C
C
Kaiser
K
K
Peyron
F
F
Picot
S
S
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004351:Drug Resistance; D017125:Genes, Protozoan; D005819:Genetic Markers; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D017931:DNA Primers; D005819:Genetic Markers