Significance of increasing adhesion of cord blood hematopoietic cells and a new method: platelet microparticles.
Hematopoietic recovery after transplantation with cord blood (CB) is slower than with bone marrow (BM) and mobilized peripheral blood. Adhesion molecules (AMs) on hematopoietic cells are involved in hematopoietic cells' homing. It may be possible to enhance CB CD34+ cells engraftment by increasing their expressions of AM. Twenty-three patients with childhood acute leukemia treated with unrelated CBT were studied. It was found that the time to neutrophil recovery correlated with CXCR4 and the time to platelet recovery correlated with both CD62L and CXCR4. Platelet microparticles (PMPs) carry some AMs such as aIIb b (CD41), P-selectin (CD62P), and CXCR4, CD34+ cells express platelet-binding antigens (CD162 and CD11b). It was found that AMs were increased dramatically on CD34+ cells surface in the presence of PMPs, and CD34+ cells covered with PMPs adhered better to human umbilical vein endothelial cells and fibronectin. These findings suggested that PMPs could increase adhesion of donor's cells to host BM in CBT.
Liu
Bin
B
Guangzhou Cord Blood Bank, Guangzhou Mothers and Children Hospital, Guangzhou, China.
Liao
Can
C
Chen
Jingsong
J
Gu
Shaoling
S
Wu
Shaoqin
S
Xu
Zunpeng
Z
D000293:Adolescent; D018952:Antigens, CD34 / Q000032:analysis; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005353:Fibronectins / Q000378:metabolism; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D019041:L-Selectin / Q000032:analysis; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D019718:Receptors, CXCR4 / Q000032:analysis
D018952:Antigens, CD34; D005353:Fibronectins; D019718:Receptors, CXCR4; D019041:L-Selectin