[Comparison of auramine-rhodamine and Erlich-Ziehl-Neelsen staining methods for the diagnosis of tuberculosis].
The aim of this study was to compare auramin-rhodamine (A-R) and Erlich-Ziehl-Neelsen (EZN) staining techniques for the diagnosis of tuberculosis. Of 311 sputum samples collected from active pulmonary tuberculosis patients and tuberculosis-suspected patients, 103 (33%) were found culture positive. In the direct microscopic examination of EZN stained smears, 86.4% (89/103) of culture positive samples, and 3.8% (8/208) of culture negative samples yielded asido-resistant bacteria, while these rates were 74.8% (77/103) and 11.5% (24/208) for A-R staining method. When culture was accepted as reference method, the specificity and sensitivity of the staining techniques were found as 88.5% and 74.8% for A-R, and 96.2% and 86.4% for EZN, respectively. As a result, it was concluded that, the use of A-R staining alone, could not be an alternative method to EZN staining.
Refik Saydam Hifzissihha Merkez Başkanliği, Tüberküloz Referans ve Araştirma Laboratuvari, Ankara.
Gülnur
G
Tarhan
Lale
L
Ordulu
Feyzullah
F
Gümüşlü
Ismail
I
Ceyhan
Salih
S
Cesur
D001576:Benzophenoneidum; D004396:Coloring Agents; D005456:Fluorescent Dyes; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012235:Rhodamines; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013194:Staining and Labeling / Q000379:methods*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D001576:Benzophenoneidum