12E2: a cloned murine dermal cell with features of dermal dendrocytes and capacity to produce pathologic changes resembling early Kaposi's sarcoma.
Factor XIIIa-positive dendrocytes are abundant within the dermis and have been implicated in the pathogenesis of various disorders, including AIDS-related Kaposi's sarcoma. Purified cultures of factor XIIIa-positive normal dermal dendrocytes have not as yet been achieved. 12E2 is a cloned cell line derived from superficial murine dermis where factor XIIIa-positive dendrocytes are abundant. Subconfluent cultures of 12E2 demonstrate polydendritic cell contours with thin, elongated membranous projections. These cells express Factor XIIIa and VCAM-1 by immunohistochemistry and by Western blot analysis of 12E2 cell lysates. 12E2 cells also constitutively express the Langerhans-cell-related epitope DEC-205, detected by NLDC-145 antibody and the CD80 co-stimulatory molecule, as well as Ia antigen on exposure to interferon-gamma. Cells so treated exhibit significant ability to present alloantigens in vitro. 12E2 cells are shown to express mRNA for numerous cytokines, including interleukin (IL)-1alpha, IL-1beta, IL-5, IL-6, IL-7, tumor necrosis factor-alpha and granulocyte macrophage-colony stimulating factor, by reverse-transcriptase polymerase chain reaction followed by Southern blot hybridization. Microinjection of 12E2 cells, but not 3T3control fibroblasts, into footpads of syngeneic and SCID mice results in lesions that mimic the histology and immunohistochemistry of human Kaposi's sarcoma. In aggregate, these data indicate that 12E2 cells 1) share lineage characteristics with factor XIIIa-positive dermal dendrocytes, 2) produce mRNA for numerous cytokines and are cytokine responsive to interferon-gamma, and 3) behave in vivo in a manner that resembles Kaposi's sarcoma, a condition known to involve proliferation of human dermal dendrocytes.
Department of Pathology, Thomas Jefferson University, Jefferson Medical College, Philadelphia, PA 19107-6799, USA.
Masatoshi
M
Deguchi
Diana
D
Whitaker-Menezes
Stephen C
SC
Jones
Setsuya
S
Aiba
Satoshi
S
Nakagawa
Hachiro
H
Tagami
Robert
R
Korngold
George F
GF
Murphy
D016475:3T3 Cells; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D019070:Cell Lineage; D002999:Clone Cells; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D020405:Dermis / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D026122:Factor XIIIa / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000096:biosynthesis; D051379:Mice; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000473:pathology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D026122:Factor XIIIa