Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identification of S. enterica serovar Typhimurium.
Lim
Young-Hee
YH
Department of Clinical Laboratory Science, College of Health Sciences, Korea University, Seoul 136-703, Korea.
Hirose
Kenji
K
Izumiya
Hidemasa
H
Arakawa
Eiji
E
Takahashi
Hideyuki
H
Terajima
Jun
J
Itoh
Ken-ichiro
K
Tamura
Kazumichi
K
Kim
Sung-Il
SI
Watanabe
Haruo
H
D016133:Polymerase Chain Reaction / Q000379:methods*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*