Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.
Active macromolecular transport between the nucleus and cytoplasm proceeds through nuclear pore complexes and is mostly mediated by transport receptors of the importin beta-superfamily. Here we identify exportin 6 (Exp6) as a novel family member from higher eukaryotes and show that it mediates nuclear export of profilin.actin complexes. Exp6 appears to contact primarily actin, but the interaction is greatly enhanced by the presence of profilin. Profilin thus functions not only as the nucleotide exchange factor for actin, but can also be regarded as a cofactor of actin export and hence as a suppressor of actin polymerization in the nucleus. Even though human and Drosophila Exp6 share only approximately 20% identical amino acid residues, their function in profilin.actin export is conserved. A knock-down of Drosophila Exp6 by RNA interference abolishes nuclear exclusion of actin and results in the appearance of nuclear actin paracrystals. In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei.
ZMBH, INF 282, D-69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Theis
T
Stüven
Enno
E
Hartmann
Dirk
D
Görlich
D000199:Actins / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003285:Contractile Proteins; D004330:Drosophila; D029721:Drosophila Proteins; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D028884:Karyopherins / Q000235:genetics* / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D051304:Profilins; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D014714:Vertebrates
D000199:Actins; D003285:Contractile Proteins; D029721:Drosophila Proteins; D028884:Karyopherins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C495737:chic protein, Drosophila; C000588850:ebo protein, Drosophila