Production of polyclonal antisera against feline immunoglobulin E and its use in an ELISA in cats with experimentally induced asthma.
Serum samples from six cats with experimentally induced asthma were used to purify feline IgE using gel filtration and affinity chromatography. The resultant IgE, evaluated for purity by immunoelectrophoresis (IEP) and reactivity by Prausnitz-Kustner (PK) testing, was used to develop polyclonal rabbit anti-feline IgE antisera. Using reverse cutaneous anaphylaxis (RCA), the antisera were determined to be specific for feline IgE. The polyclonal rabbit anti-feline IgE antiserum was then validated in an allergen-specific ELISA. Serum samples from an additional five asthmatic cats sensitized with Bermuda grass allergen (BGA) were evaluated prior to sensitization, after parenteral sensitization, and after aerosol sensitization and challenge. A significant increase in serum BGA-specific IgE was noted over time.
Norris
C R
CR
Departments of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA 95616-8734, USA. cnorris@ucdavis.edu
Decile
K C
KC
Byerly
J R
JR
Berghaus
R D
RD
Schelegle
E S
ES
Hyde
D M
DM
Gershwin
L J
LJ
D000485:Allergens / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D001249:Asthma / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D002850:Chromatography, Gel / Q000662:veterinary; D031744:Cynodon / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007106:Immune Sera / Q000276:immunology; D007122:Immunoelectrophoresis / Q000662:veterinary; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; C026408:anti-IgE antibodies; D007073:Immunoglobulin E