Engineered retargeting of viral RNA replication complexes to an alternative intracellular membrane.
Positive-strand RNA virus replication complexes are universally associated with intracellular membranes, although different viruses use membranes derived from diverse and sometimes multiple organelles. We investigated whether unique intracellular membranes are required for viral RNA replication complex formation and function in yeast by retargeting protein A, the Flock House virus (FHV) RNA-dependent RNA polymerase. Protein A, the only viral protein required for FHV RNA replication, targets and anchors replication complexes to outer mitochondrial membranes in part via an N-proximal sequence that contains a transmembrane domain. We replaced the FHV protein A mitochondrial outer membrane-targeting sequence with the N-terminal endoplasmic reticulum (ER)-targeting sequence from the yeast NADP cytochrome P450 oxidoreductase or inverted C-terminal ER-targeting sequences from the hepatitis C virus NS5B polymerase or the yeast t-SNARE Ufe1p. Confocal immunofluorescence microscopy confirmed that protein A chimeras retargeted to the ER. FHV subgenomic and genomic RNA accumulation in yeast expressing ER-targeted protein A increased 2- to 13-fold over that in yeast expressing wild-type protein A, despite similar protein A levels. Density gradient flotation assays demonstrated that ER-targeted protein A remained membrane associated, and in vitro RNA-dependent RNA polymerase assays demonstrated an eightfold increase in the in vitro RNA synthesis activity of the ER-targeted FHV RNA replication complexes. Electron microscopy showed a change in the intracellular membrane alterations from a clustered mitochondrial distribution with wild-type protein A to the formation of perinuclear layers with ER-targeted protein A. We conclude that specific intracellular membranes are not required for FHV RNA replication complex formation and function.
Department of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
David J
DJ
Miller
Michael D
MD
Schwartz
Billy T
BT
Dye
Paul
P
Ahlquist
D000595:Amino Acid Sequence; D000818:Animals; D004721:Endoplasmic Reticulum / Q000821:virology; D007425:Intracellular Membranes / Q000821:virology*; D051379:Mice; D008928:Mitochondria / Q000821:virology; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D012324:RNA-Dependent RNA Polymerase