Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance.
Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 +/- 16.55 and 140.20 +/- 22.27 microl/min in male and female C57BL/6J mice, respectively. Total or (5/6) nephrectomy reduced inulin clearance to 0 or 32.80 +/- 9.32 microl/min, respectively. Conversely, diabetes mellitus induced by streptozotocin was associated with increased GFR. The other approach measured urinary inulin clearance using intraperitoneal microosmotic pumps to deliver FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 +/- 26.56 and 157.36 +/- 20.02 microl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated nonisotopic measurement of inulin clearance in conscious mice.
Qi
Zhonghua
Z
Department of Medicine, Vanderbilt University, Nashville, TN 37212, USA. zhonghua.qi@vanderbilt.edu
Whitt
Irene
I
Mehta
Amit
A
Jin
Jianping
J
Zhao
Min
M
Harris
Raymond C
RC
Fogo
Agnes B
AB
Breyer
Matthew D
MD
D000818:Animals; D003243:Consciousness; D003921:Diabetes Mellitus, Experimental / Q000652:urine; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage; D005456:Fluorescent Dyes / Q000008:administration & dosage; D005919:Glomerular Filtration Rate; D007267:Injections; D007444:Inulin / Q000008:administration & dosage / Q000097:blood / Q000652:urine*; D007677:Kidney Function Tests / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009392:Nephrectomy
D005456:Fluorescent Dyes; D007444:Inulin; D016650:Fluorescein-5-isothiocyanate