Active internalization of the Penicillium chrysogenum antifungal protein PAF in sensitive aspergilli.
The Penicillium chrysogenum antifungal protein PAF inhibits the growth of various filamentous fungi. In this study, PAF was found to localize to the cytoplasm of sensitive aspergilli by indirect immunofluorescence staining. The internalization process required active metabolism and ATP and was prevented by latrunculin B, suggesting an endocytotic mechanism.
Oberparleiter
Christoph
C
Department of Molecular Biology, University of Innsbruck, Innsbruck, Austria.
Kaiserer
Lydia
L
Haas
Hubertus
H
Ladurner
Peter
P
Andratsch
Manfred
M
Marx
Florentine
F
D000255:Adenosine Triphosphate / Q000378:metabolism; D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001230:Aspergillus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010407:Penicillium; D010408:Penicillium chrysogenum / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines
D000935:Antifungal Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005656:Fungal Proteins; C097832:PAF protein, Penicillium chrysogenum; D013844:Thiazoles; D053778:Thiazolidines; D000255:Adenosine Triphosphate; C037068:latrunculin B