Multiphoton multifocal microscopy exploiting a diffractive optical element.
Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds.
Sacconi
L
L
University of Trento, Via Sommarive 14, 38050 Povo, Trento, Italy.
Froner
E
E
Antolini
R
R
Taghizadeh
M R
MR
Choudhury
A
A
Pavone
F S
FS