IL-12/GM-CSF coadministration in an SIV DNA prime/protein boost protocol enhances Gag-specific T cells but not virus-specific neutralizing antibodies in rhesus macaques.
Coadministration of pVecB7, a replication-defective SIV DNA vaccine, with interleukin-12 and GM-CSF expression plasmids, induced markedly enhanced control of viral replication and disease-free survival in macaques challenged intrarectally with pathogenic SIVsmE660. Protective mechanisms correlated with broader cell-mediated immune responses to the first two-thirds of the SIV Gag protein and possibly with enhanced SIVsmE660 antibody neutralization at set point, but not with pre- or early postchallenge SIVsmE660 neutralizing antibody production.
O'Neill
Eduardo
E
Department of Microbiology and Medical Zoology, Unit of Comparative Medicine, University of Puerto Rico School of Medicine, San Juan, Puerto Rico 00936.
Bostik
Vanda
V
Montefiori
David C
DC
Kraiselburd
Edmundo
E
Villinger
François
F
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D015683:Gene Products, gag / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage* / Q000276:immunology; D018664:Interleukin-12 / Q000008:administration & dosage* / Q000276:immunology; D008253:Macaca mulatta; D018449:SAIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
D000914:Antibodies, Viral; D015683:Gene Products, gag; D018449:SAIDS Vaccines; D019444:Vaccines, DNA; D018664:Interleukin-12; D016178:Granulocyte-Macrophage Colony-Stimulating Factor