Hyperthermia enhances tumor necrosis factor alpha-induced apoptosis of a human gastric cancer cell line.
We have investigated the effects of hyperthermia on the apoptosis induced by tumor necrosis factor alpha (TNF-alpha). Confluent monolayers of human gastric cancer cell line MKN45 were either treated or untreated with hyperthermia for 1 h. The cells were subsequently stimulated with TNF-alpha. A 24-h incubation with TNF-alpha did not affect cell viabilities; however, pretreatment with hyperthermia significantly enhanced the level of apoptosis induced by TNF-alpha. Pretreating MKN45 cells with hyperthermia (42.0 degrees C) significantly inhibited the TNF-alpha-induced increase in the binding activity of NF-kappaB to DNA. This study suggests that hyperthermia can inhibit the TNF-alpha-induced NF-kappaB activation and that hyperthermia renders human gastric cancer cells susceptible to the TNF-alpha-induced apoptosis, possibly via inhibition of the NF-kappaB pathway.
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. s-kokura@mpd.biglobe.ne.jp
Satoshi
S
Kokura
Norimasa
N
Yoshida
Miho
M
Ueda
Eiko
E
Imamoto
Takeshi
T
Ishikawa
Tomohisa
T
Takagi
Yuji
Y
Naito
Takeshi
T
Okanoue
Toshikazu
T
Yoshikawa
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D053938:DNA Fragmentation; D005334:Fever; D006801:Humans; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha