Karyotyping of human synaptonemal complexes by cenM-FISH.
The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
Oliver-Bonet
Maria
M
Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cel.lular, Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain. moliver@servet.uab.es
Liehr
Thomas
T
Nietzel
Angela
A
Heller
Anita
A
Starke
Heike
H
Claussen
Uwe
U
Codina-Pascual
Montserrat
M
Pujol
Aïda
A
Abad
Carlos
C
Egozcue
Josep
J
Navarro
Joaquima
J
Benet
Jordi
J
D002503:Centromere / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007246:Infertility; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000235:genetics*