Identification of a gene engineering antibody against cystic echinococcosis in liver.
OBJECTIVE
To identify a gene engineering antibody against cystic echinococcosis in liver.


METHODS
A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selection technique with the recombinant antigen on solid surface. The positive clones were demonstrated by ELISA and their DNA sequences were also determined.


RESULTS
The DNA sequence data showed that the antibody gene is composed of 768 bp. In addition, a specific combination capacity with recombinant Echinococcus granulosus antigen B (r-EgB) was demonstrated by ELISA.


CONCLUSIONS
The obtained gene engineering antibody against r-EgB may have potential implications in immunological treatment and drug targeting delivery.
Chen
Xin-Hua
XH
Xinjiang Hydatid Clinical Research Institute, No.1 Liyushan Road, Urumqi 830054, China.
Wen
Hao
H
Zhang
Yao-Xin
YX
Feng
Xiao-Hui
XH
Lu
Xiao-Mei
XM
Ma
Dong
D
D000818:Animals; D000909:Antibodies, Helminth / Q000235:genetics*; D000918:Antibody Specificity; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000235:genetics* / Q000276:immunology*; D005818:Genetic Engineering; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019151:Peptide Library; D017422:Sequence Analysis, DNA
D000909:Antibodies, Helminth; D007135:Immunoglobulin Variable Region; D019151:Peptide Library