Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice.
Naked plasmid DNA (pDNA) vaccine expressing herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) was tested for protective activity against acute HSV-1 infection in mice. The pDNA was intravenously injected into Balb/c mice via their tail vein under high pressure, and the vaccination was performed two times at an interval of 7 days. The gB gene vaccination significantly protected the mice from subsequent intraperitoneal challenge with a lethal dose of HSV-1, which killed all the animals given control plasmid or saline. The protective activity was correlated with the dose of the plasmid inoculated, the survival rate reaching 83% in mice vaccinated with 5 microg of pDNA. The vaccinated mice were also protected from latent HSV infection. The immunized mice showed significant elevation in neutralizing antibody against HSV-1 as well as serum levels of interleukin-12 (IL-12) and interferon-gamma (IFN-gamma). When mice were immunized with 5 microg of an Epstein-Barr virus (EBV)-based plasmid vector harboring the gB, the cytotoxic T lymphocytes (CTLs) activity and proliferative response for HSV-1 were also induced. The results strongly suggest that intravenous immunization of naked pDNA may induce humoral and cellular immune responses against the virus, leading to a significant prophylactic outcome against HSV-1 infection in mice.
Cui
F-D
FD
Department of Microbiology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Asada
H
H
Kishida
T
T
Itokawa
Y
Y
Nakaya
T
T
Ueda
Y
Y
Yamagishi
H
H
Gojo
S
S
Kita
M
M
Imanishi
J
J
Mazda
O
O
D000818:Animals; D000917:Antibody Formation; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods*; D006561:Herpes Simplex / Q000276:immunology* / Q000628:therapy*; D018259:Herpesvirus 1, Human; D004854:Herpesvirus 4, Human / Q000235:genetics; D007111:Immunity, Cellular; D007275:Injections, Intravenous; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*; D017735:Virus Latency
D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D018664:Interleukin-12; D007371:Interferon-gamma