Highly effective poly(ethylene glycol) architectures for specific inhibition of immune receptor activation.
Architectural features of synthetic ligands were systematically varied to optimize inhibition of mast cell degranulation initiated by multivalent crossing of IgE-receptor complexes. A series of ligands were generated by end-capping poly(ethylene glycol) (PEG) polymers and amine-based dendrimers with the hapten 2,4-dinitrophenyl (DNP). These were used to explore the influence of polymeric backbone length, valency, and hapten presentation on binding to anti-DNP IgE and inhibition of stimulated activation of RBL cells. Monovalent MPEG(5000)-DNP (IC(50) = 50 nM), bivalent DNP-PEG(3350)-DNP (IC(50) = 8 nM), bismonovalent MPEG(5000)-DNP(2) (IC(50) = 20 nM), bisbivalent DNP(2)-PEG(3350)-DNP(2) (IC(50) = 3nM) and DNP(4)-dendrimer ligands (IC(50) = 50 nM) all effectively inhibit cellular activation caused by multivalent antigen, DNP-bovine serum albumin. For different DNP ligands, we provide evidence for more effective inhibition due to (i) preferential formation of intra-IgE cross-links by bivalent ligands of sufficient length, (ii) self-association of monovalent ligands with longer tails, and (iii) higher probability of binding for bisvalent ligands. We also show that larger DNP(16)-dendrimers of higher valency trigger degranulation by cross-linking IgE-receptor complexes, whereas smaller DNP-dendrimers are inhibitory. Thus, features of synthetic ligands can be manipulated to control receptor occupation, aggregation, and inhibition of the cellular response.
Baird
Emily J
EJ
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853-1301, USA.
Holowka
David
D
Coates
Geoffrey W
GW
Baird
Barbara
B
D000588:Amines / Q000138:chemical synthesis; D000818:Animals; D001665:Binding Sites; D015550:Cell Degranulation; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D004136:Dinitrobenzenes / Q000138:chemical synthesis; D006241:Haptens / Q000737:chemistry; D007073:Immunoglobulin E / Q000737:chemistry; D008024:Ligands; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000494:pharmacology*; D051381:Rats; D017455:Receptors, IgE / Q000037:antagonists & inhibitors* / Q000378:metabolism*
D000588:Amines; D003432:Cross-Linking Reagents; D004136:Dinitrobenzenes; D006241:Haptens; D008024:Ligands; D017455:Receptors, IgE; D007073:Immunoglobulin E; D011092:Polyethylene Glycols