Simultaneous determination of tryptophan and glutathione in individual rat hepatocytes by capillary zone electrophoresis with electrochemical detection at a carbon fiber bundle--Au/Hg dual electrode.
A method for single-cell analysis was developed by combining capillary zone electrophoresis (CZE) with electrochemical detection (ECD) using a dual electrode consisting of two different types of electrode material (carbon fiber and Au/Hg). In this method, the parallel mode was used. Different potentials were applied to both electrodes of the dual electrode. Tryptophan and glutathione, which could not be simultaneously detected by normal CZE-ECD, could be simultaneously and selectively detected by CZE-ECD at the dual electrode in one run, respectively. The CZE-ECD system with the dual electrode was applied to determine them in individual rat hepatocytes.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. wenrujin@jn-public.cninfo.net
Wenrui
W
Jin
Xiujun
X
Li
Ning
N
Gao
D000818:Animals; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D002395:Catecholamines / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D022781:Hepatocytes / Q000737:chemistry*; D008628:Mercury / Q000737:chemistry*; D051381:Rats; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism; D014364:Tryptophan / Q000032:analysis* / Q000378:metabolism
D000077482:Carbon Fiber; D002395:Catecholamines; D012701:Serotonin; D002244:Carbon; D006046:Gold; D014364:Tryptophan; D008628:Mercury; D005978:Glutathione