Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS: identification of ceramides containing sphingadienine.
Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases. Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.
Institut National de la Santé et de la Recherche Médicale U495, Laboratoire de Neurochimie, Hopital de la Salpetriere, 75651 Paris 13, France.
Benoit
B
Colsch
Carlos
C
Afonso
Iuliana
I
Popa
Jacques
J
Portoukalian
Françoise
F
Fournier
Jean-Claude
JC
Tabet
Nicole
N
Baumann
D000818:Animals; D001923:Brain Chemistry; D002518:Ceramides / Q000737:chemistry*; D004983:Ethanolamines / Q000737:chemistry*; D005699:Galactosylceramides / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry*; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization; D013110:Sphingosine / Q000737:chemistry
D002518:Ceramides; D004983:Ethanolamines; D005699:Galactosylceramides; D005732:Gangliosides; D006028:Glycosphingolipids; C003337:sphingadienine; D013110:Sphingosine