Studies on biodegradation and release of gentamicin sulphate from interpenetrating network hydrogels based on poly(acrylic acid) and gelatin: in vitro and in vivo.
Interpenetrating network hydrogels (IPNs) based on poly(acrylic acid) and gelatin (Ge) were evaluated for in vitro and in vivo biodegradation and in vivo release of gentamicin sulphate. In vitro and in vivo degradation studies demonstrated that with the increase of acrylic acid content in the polymer, the rate of degradation decreases, and a reverse phenomenon was observed with increasing Ge content in the hydrogel. The rate of in vivo degradation was much lower than in vitro degradation. Incorporation of gentamicin sulphate in hydrogel further reduces their degradation. In vitro and in vivo drug release profile showed a burst effect, followed by controlled release. Drug concentration was measured in the local skin tissue, blood serum, kidney, liver and spleen. The local skin tissue concentration of 50% and 100% gentamicin sulphate, loaded full IPNs (i.e., Ax-1 and Ax-2), was found to be higher (20+/-2mug/g) than the minimum bactericidal concentration for Staphylococcus aureus (1.2mug/g) and Pseudomonas aeruginosa (10mug/g), respectively, for a study time of 60 days.
Centre for Biomedical Engineering, Indian Institute of Technology (IIT), Hauz Khas, New Delhi, 110016, India.
Mohammad
M
Changez
Veena
V
Koul
B
B
Krishna
Amit Kumar
AK
Dinda
Veena
V
Choudhary
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001673:Biodegradation, Environmental; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004343:Drug Implants; D005780:Gelatin / Q000737:chemistry*; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D005976:Glutaral / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008422:Materials Testing; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D004343:Drug Implants; D005839:Gentamicins; D020100:Hydrogels; C006903:carbopol 940; D005780:Gelatin; D005976:Glutaral