Naphthalene exposure: effects on gene expression and proliferation in human cord blood cells.
In this study, the effect of naphthalene on the induction of apoptosis and gene expression profiles in cord blood cells has been evaluated, as well as its activity on the BCL-2 related protein expression. After 6, 24, and 48 h of exposure to naphthalene (500 microM), a decrease in cell death was observed: the cells became more resistant to the toxicant and capable of surviving after the treatment. A Western blot analysis revealed an overexpression of BCL-2, c-JUN, c-FOS, and RAF-1 proteins, which are involved in the antiapoptotic response and in the regulation of cell growth, differentiation, and development. Furthermore, macroarray analysis showed that naphthalene modified cord blood gene expression, inducing IL-8 precursor and T-cell transcription factor and decreasing the level of RNA-binding protein FUS/TLS.
Diodovich
Cristina
C
Laboratory of Hematotoxicology, ECVAM, Institute for Health and Consumer Protection, J.R.C., 21020 Ispra, Italy.
Malerba
Ilaria
I
Bowe
Gerard
G
Acquati
Francesco
F
Bianchi
Marco Giorgio
MG
Taramelli
Roberto
R
Parent-Massin
Dominique
D
Gribaldo
Laura
L
D017209:Apoptosis / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009281:Naphthalenes / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
D018797:Cell Cycle Proteins; D016209:Interleukin-8; D009281:Naphthalenes; D014157:Transcription Factors; C031721:naphthalene