Immunohistochemical study of surgically excised choroidal neovascular membranes.
We carried out an immunohistochemical investigation of the choroidal neovascular membranes from 12 eyes surgically excised as a result of age-related macular degeneration (n = 6) or idiopathic choroidal neovascularization (n = 6). Immunohistochemical staining was performed with antibodies specific for basic transcriptional element binding protein-2, actin or smooth muscle cell 1. In all membranes, the endothelial cells and stromal components around the vessels were immunoreactive for expression of basic transcriptional element binding protein-2, while immunoreactive expression of actin and smooth muscle cell type 1 was found in the surrounding stromal cells. These results suggest that basic transcriptional element binding protein-2, a zinc finger transcription factor, may contribute to the establishment of the choroidal neovascularization observed in the pathogenesis of age-related macular degeneration and idiopathic choroidal neovascularization.
Department of Ophthalmology, Gunma University School of Medicine, Gunma, Japan.
T
T
Sato
T
T
Kanda
T
T
Iida
T
T
Takahashi
S
S
Kishi
Y
Y
Hoshino
D000199:Actins / Q000096:biosynthesis; D000328:Adult; D000367:Age Factors; D000368:Aged; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000378:metabolism*; D020256:Choroidal Neovascularization; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051741:Kruppel-Like Transcription Factors; D008268:Macular Degeneration / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D015534:Trans-Activators / Q000096:biosynthesis; D000098550:Structural Maintenance of Chromosome Protein 1
D000199:Actins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors; D015534:Trans-Activators; D000098550:Structural Maintenance of Chromosome Protein 1