Temporal and spatial assembly of lipid droplet-associated proteins in 3T3-L1 preadipocytes.
This study aimed to investigate the relationship between newly formed lipid droplets and lipid droplet surface proteins, including perilipin, adipose differentiation related protein (ADRP), and p200 kDa protein (p200) in 3T3-L1 preadipocytes, during lipogenesis. Sterol ester was used to induce nascent lipid droplets in 3T3-L1 preadipocytes and the sequence of lipids and lipid droplet surface proteins was studied using a combination of immunohistochemistry and Nile red staining/Oil red O. We demonstrated that, although most growing lipid droplets appeared to have a lipid core surrounded by a fluorescent rim of ADRP, perilipin, and p200, tiny protein aggregates of ADRP, perilipin, or p200 could also be found to occur in the absence of lipid accumulation. In addition, ADRP associated with nascent lipid droplets prior to that of perilipin or p200. We provide evidence that lipid droplet surface proteins, especially ADRP and perilipin, are important in serving as a nucleation center for the assembly of lipid to form nascent lipid droplets.
Wang
Seu-Mei
SM
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, 1-1 Jen-Ai Road, 100 Taipei, Taiwan. smwang@ha.mc.ntu.edu.tw
Hwang
Ran-Der
RD
Greenberg
Andrew S
AS
Yeo
Hui-Ling
HL
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002352:Carrier Proteins; D008055:Lipids / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D013234:Stem Cells / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D002352:Carrier Proteins; D008055:Lipids; D008565:Membrane Proteins; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins; C000602983:Plin2 protein, mouse