Nuclear inheritance of erythromycin resistance in human cells: new class of mitochondrial protein synthesis mutants.
The characterization of two new erythromycin-resistant mutants of HeLa cells is described. The strains ERY2305 and ERY2309 both exhibited resistance to erythromycin in growth assays and cell-free mitochondrial protein synthesis assays. The erythromycin resistance phenotype could not be transferred by cybridization. The mutation appeared to be encoded in the nucleus and inherited as a recessive trait. These two mutants, therefore, represent a new class of erythromycin-resistant mutants in human cells that is distinct from the cytoplasmically inherited mutation in strain ERY2301 described previously.
Department of Microbiology, California College of Medicine, University of California, Irvine, California 92717, USA.
C J
CJ
Doersen
E J
EJ
Stanbridge
D002455:Cell Division; D002467:Cell Nucleus / Q000235:genetics*; D002469:Cell Separation; D002470:Cell Survival; D002474:Cell-Free System; D002877:Chromosomes, Human / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D009154:Mutation / Q000235:genetics*
D024101:Mitochondrial Proteins; D004917:Erythromycin