A novel gene encoding an enzyme that degrades a polysaccharide from the sheath of Sphaerotilus natans.
A gene encoding an enzyme that is able to depolymerize the basic polysaccharide prepared from the sheath of Sphaerotilus natans was identified in a sheath-degrading bacterium, Paenibacillus koleovorans. The gene was constructed from 2217 bp coding for 738 amino acids, including the signal sequence of 34 amino acids. No closely related protein or gene was indicated by a homology search. The gene was expressed in Escherichia coli as a glutathione S-transferase fusion protein. The fusion protein depolymerized the sheath polysaccharide into an oligosaccharide, introducing an unsaturated sugar residue, suggesting that the gene codes for a polysaccharide lyase acting on a basic polysaccharide.
Takeda
Minoru
M
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National University, Tokiwadai, Hodogaya, Yokohama, Japan. mtake@ynu.ac.jp
Nishina
Katsuya
K
Hanaoka
Yuji
Y
Higo
Motoko
M
Terui
Mutsumi
M
Suzuki
Ichiro
I
Koizumi
Jun-ichi
J
D001407:Bacillus / Q000201:enzymology; D003001:Cloning, Molecular / Q000379:methods; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008190:Lyases / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D042744:Sphaerotilus / Q000737:chemistry*
D004798:Enzymes; D011135:Polysaccharides, Bacterial; D011993:Recombinant Fusion Proteins; D008190:Lyases