Advanced cancer genetics in neurosurgical research.
RAPID ADVANCES IN the technology used to study nucleic acids have revealed a great deal regarding the underlying biology of cancer. Most cancers arise as a result of chromosomal rearrangements and deoxyribonucleic acid mutations that lead to the activation of proto-oncogenes and loss of function of tumor suppressor genes. There are a number of different molecular routes that lead to these common goals, necessitating several different techniques of mutational analysis. Although many of these techniques can be difficult in practice, most are conceptually simple. We discuss several of the current techniques in cytogenetics and molecular genetics that are widely used in cancer biology laboratories. Understanding the molecular events that lead to cancer should allow the future development of targeted, nontoxic therapeutics similar to modern-day antibiotics. These technologies are being progressively applied in clinical neurosurgery, where they will be used to detect, diagnose, stratify, and treat cancers of the nervous system. High demand from an increasingly educated patient population means that neurosurgeons will need to be familiar with many of these techniques.
Division of Neurosurgery, Hospital for Sick Children, Arthur and Sonia Labatt Brain Tumor Research Center, University of Toronto, Toronto, Ontario, Canada. mike.taylor@annexgraphics.com
Michael D
MD
Taylor
Corey
C
Raffel
Jane
J
Bayani
Paula
P
Marrano
Todd G
TG
Mainprize
Jeremy
J
Squire
D020732:Cytogenetic Analysis / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D009423:Nervous System Neoplasms / Q000235:genetics*; D009493:Neurosurgery / Q000379:methods*