Metabolic oligosaccharide engineering as a tool for glycobiology.
Oligosaccharides transact information exchange at the cell surface and modulate the activities and distribution of proteins within cells. Recently, the ability to modify monosaccharide structures within cellular glycans through metabolic processes has offered a new avenue for biological studies. The technique of metabolic oligosaccharide engineering has been used to disrupt glycan biosynthesis, chemically modify cell surfaces, probe metabolic flux inside cells, and to identify specific glycoprotein subtypes from the proteome.
Department of Chemistry, Howard Hughes Medical Institute, University of California, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Danielle H
DH
Dube
Carolyn R
CR
Bertozzi
D000818:Animals; D001709:Biotechnology / Q000379:methods; D006001:Glycoconjugates; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry*; D040901:Proteomics
D006001:Glycoconjugates; D006023:Glycoproteins; D009844:Oligosaccharides