A generalized transducing thiophage (TPC-1) of a facultative sulfur chemolithotrophic bacterium, Bosea thiooxidans CT5, of alpha-Proteobacteria, isolated from Indian soil.
We have isolated and characterized a double-stranded DNA bacteriophage (TPC-1) of Bosea thiooxidans, a facultative sulfur chemolithotrophic bacterium. The name 'thiophage' is introduced for phage(s) infecting sulfur chemolithotrophic bacteria. Electron micrographs showed the phage particle with an icosahedral head and a very short wedge-like tail. TPC-1 is classified as the C1 morphotype of the Podoviridae family. Restriction map and terminal ends detection by end fill labeling of the TPC-1 genomic DNA showed that the genome is linear with 5' protruding cohesive termini. Contour length mapping of the DNA genome also revealed it to be a linear fragment with size ( approximately 44 kb) corresponding with the size estimated from restriction fragment analyses and proved the non-redundant nature of the linear genome topology. In colorless sulfur chemolithotrophic microorganisms, TPC-1 is the first report of a generalized transducing thiophage.
Department of Microbiology, Bose Institute, Acharya J.C. Bose Birth Centenary Building, Kankurgachhi, P-1/12, C.I.T. Scheme VII-M, Kolkata- 700 054, India.
Chirajyoti
C
Deb
Ranadhir
R
Chakraborty
Amar N
AN
Ghosh
Nitai C
NC
Mandal
Tusharmouli
T
Mukherjee
Pradosh
P
Roy
D020561:Alphaproteobacteria / Q000145:classification / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D004279:DNA, Viral / Q000032:analysis*; D007194:India; D008242:Lysogeny / Q000235:genetics; D015183:Restriction Mapping; D012988:Soil Microbiology; D013885:Thiosulfates / Q000378:metabolism*
D004279:DNA, Viral; D013885:Thiosulfates