Functional study on baculovirus anti-apoptosis genes.
Apoptosis of bollworm cell line Hz-AM1 can be delayed by transient expression of AcNPV (Autographa californica Nuclear Polihedrosis Virus) p35 gene. Acp35Z, a p35 inactivated AcNPV by inserting with LacZ gene, cannot replicate in Hz-AM1 cells. However, the replication can be rescued by co-transfection with a plasmid containing AcNPV p35 gene. It is also realized that the transient expression of AcNPV p35 gene in Hela cells can put off cell apoptosis which is induced by adding actinomycin-D. Through co-transfection and G418 screening, two anti-apoptosis cell lines named Sf9-35 and Vero-35 are established by integrating AcNPV p35 and Neo expression cassette into the cell chromosomes. The Sf9-35 enhances the yield of budded virus of AcNPV, while the Vero-35 increases the propagation of measles virus.
Institute of Virology, The College of Life Sciences, Wuhan University, Wuhan, Hubei, PR China.
Yefu
Y
Wang
Yipeng
Y
Qi
Ying
Y
Zhu
Zhida
Z
Li
Yiran
Y
Yang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D017931:DNA Primers; D003609:Dactinomycin / Q000494:pharmacology; D016158:Genes, p53; D006801:Humans; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D018411:Spodoptera; D014779:Virus Replication / Q000235:genetics
D017931:DNA Primers; D003609:Dactinomycin