Chemokine receptor CCR5 is not required for development of experimental autoimmune gastritis.
Experimental autoimmune gastritis (EAG) is a model of human autoimmune gastritis, the underlying cause of pernicious anaemia. It is characterised by gastric mononuclear cell infiltrates, destruction of parietal and zymogenic cells, and autoantibodies to parietal cell-associated H(+)/K(+) ATPase. Here, we have investigated the role of CCR5 in the development of EAG. We found that the development of EAG was not prevented in CCR5-deficient mice. Using reverse-transcriptase analysis of stomachs from normal and gastritic mice we found no difference in expression of CCR5 and its chemokine ligands MIP-1alpha, MIP-1beta, and RANTES. We also found that the CCR5 antagonist met-RANTES failed to prevent the development of EAG induced by neonatal thymectomy. These observations suggest that the CC chemokine receptor CCR5 is not essential for development of EAG.
Monash University, Department of Pathology and Immunology, Central and Eastern Clinical School, AMREP Commercial Road, Prahran, 3181 Victoria, Australia.
Judith
J
Field
Aiden C J
AC
Marshall
Paul J
PJ
Hertzog
Timothy N
TN
Wells
Frank
F
Alderuccio
Ban Hock
BH
Toh
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D065100:CCR5 Receptor Antagonists; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D003433:Crosses, Genetic; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005756:Gastritis / Q000276:immunology* / Q000473:pathology; D017506:H(+)-K(+)-Exchanging ATPase / Q000276:immunology / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010295:Parietal Cells, Gastric / Q000276:immunology; D019713:Receptors, CCR5 / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000276:immunology / Q000473:pathology
D065100:CCR5 Receptor Antagonists; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019402:Macrophage Inflammatory Proteins; C108335:RANTES, Met-; D019713:Receptors, CCR5; D004247:DNA; D017506:H(+)-K(+)-Exchanging ATPase