Association of acute parvovirus B19 infection with new onset of acute lymphoblastic and myeloblastic leukaemia.
OBJECTIVE
To investigate the association of acute parvovirus B19 infection with new onset of acute lymphoblastic and myeloblastic leukaemia.


METHODS
Cerebrospinal fluid (CSF) samples from patients with acute myelogenous leukaemia (AML) at diagnosis (n = 2) and acute lymphoblastic leukaemia (ALL) at diagnosis (n = 14) were analysed for parvovirus B19 DNA by means of nested polymerase chain reaction. In addition, samples from patients with benign intracranial hypertension (BIH) (n = 10) and hydrocephalus (n = 13) were tested as controls.


RESULTS
Four leukaemia cases were positive-common ALL (n = 2), null cell ALL (n =1), and M7 AML (n = 1)-whereas all controls were negative (Yates corrected chi(2) value, 3.97; p = 0.046; odds ratio, 16.92; confidence interval, 1.03 to 77.18). All four patients were significantly anaemic, but none was encephalitic or had evidence of central nervous system leukaemia. In three of these patients, serum tumour necrosis alpha, interferon gamma, interleukin 6, granulocyte-macrophage colony stimulating factor (range, 34.93-3800.06 pg/ml), and macrophage chemoattractant protein 1 were detectable. All of these four patients carried at least one of the HLA-DRB1 alleles, which have been associated with symptomatic parvovirus B19 infection.


CONCLUSIONS
Erythroid suppression and immune cell proliferation are both associated with B19 infection and may also be important in the pathogenesis of acute leukaemia.
Kerr
J R
JR
Department of Microbiology, Royal Brompton Hospital, Imperial College London, Sydney Street, London SW3 6NP, UK. j.kerr@ic.ac.uk
Barah
F
F
Cunniffe
V S
VS
Smith
J
J
Vallely
P J
PJ
Will
A M
AM
Wynn
R F
RF
Stevens
R F
RF
Taylor
G M
GM
Cleator
G M
GM
Eden
O B
OB
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007223:Infant; D007947:Leukemia, Megakaryoblastic, Acute / Q000821:virology*; D008297:Male; D010322:Parvoviridae Infections / Q000150:complications* / Q000276:immunology; D016732:Parvovirus B19, Human; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000821:virology*
D016207:Cytokines; D004279:DNA, Viral