Molecular detection and direct enumeration of methanogenic Archaea and methanotrophic Bacteria in domestic solid waste landfill soils.
Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the highest methane oxidizing activity, while 1 m depth buried waste showed the highest methane producing potential. All the sequences of the 1 m sample were found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We developed a modified fluorescence in situ hybridization (FISH) direct counting method for landfill samples, resulting in the detection of approx. 1% of total cells as archaeal cells (presumably methanogens). However, probe-positive cells could not be found with probes for methanotrophs by the methods.
Chen
Ann-Cheng
AC
Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan. encheng@mail.chna.edu.tw
Ueda
Kumiko
K
Sekiguchi
Yuji
Y
Ohashi
Akiyoshi
A
Harada
Hideki
H
D001105:Archaea / Q000235:genetics / Q000302:isolation & purification; D015169:Colony Count, Microbial / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D017014:Methanobacterium / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics*; D012037:Refuse Disposal / Q000379:methods; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology
D012336:RNA, Ribosomal, 16S; D012722:Sewage