Molecular assay for the identification of Setaria tundra.
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra.
Favia
Guido
G
Dipartimento di Biologia Molecolare Cellulare Animale, Università degli Studi di Camerino, via Camerini 2, 62032 Camerino (MC), Italy. guido.favia@unicam.it
Cancrini
Gabriella
G
Ferroglio
Ezio
E
Casiraghi
Maurizio
M
Ricci
Irene
I
Rossi
Luca
L
D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D003670:Deer / Q000469:parasitology*; D005260:Female; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D017182:Setaria Nematode / Q000145:classification* / Q000235:genetics; D012719:Setariasis / Q000175:diagnosis / Q000469:parasitology; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S