Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.
The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC(50), i.e. concentration leading to half-maximal activating effect of the hormone (10(-11)-10(-7) M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.
Kuznetsova
L
L
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr. 44, 194223, St. Petersburg, Russia.
Shpakov
A
A
Rusakov
Yu
Y
Plesneva
S
S
Bondareva
V
V
Pertseva
M
M
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D005399:Fishes; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology*; D007798:Lampreys; D008974:Mollusca; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine
D007328:Insulin; D011972:Receptor, Insulin; D000262:Adenylyl Cyclases