[Organization and differential staining of chromosomes from the endomitotic nucleus of trophocytes Calliphora erythrocephala (Diptera: Calliphoridae)].
The structure of primary polytene chromosomes and general architecture of nurse cell nuclei was studied in Calliphora erythrocephala using various methods of differential chromosome banding(G-, R-, C-banding; Ag- and DAPI staining), chromospecific DNA probes and fluorescence in situ hybridization. This analysis revealed differential compaction of particular chromosome regions. The localization of material of polytene chromosome 6 is retained after its rearrangement and the formation of the internal reticular structure of the nucleus. Polytene chromosomes of ovarian nurse cells were shown to have blocks of dense compact material; some of them were more intensely stained by AgNO3. The dynamics of the nucleolus formation was traces at all stages of chromosome polytenization in the C. erythrocephala nurse cells.
Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050 Russia.
I E
IE
Vasserlauf
T V
TV
Anan'ina
M F
MF
Unger
T V
TV
Karamysheva
N N
NN
Mel'nikova
N B
NB
Rubtsov
V N
VN
Stegniĭ
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D020223:Chromosome Painting; D015342:DNA Probes; D004175:Diptera / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis
D015342:DNA Probes