Anti malondialdehyde-adduct immunological response as a possible marker of successful aging.
Contrasting results have been obtained by various researchers about oxidative markers of aging. In this study, a healthy over-90-year-old population was examined for various plasma oxidative biomarkers and compared with a healthy population of blood donors (age range 23-66). Plasma malondialdehyde (MDA), evaluated by means of the thiobarbituric acid test, was significantly higher in the over-90-year-old population, confirming the presence of increased lipoperoxidation in old age. The antibody titre against MDA-protein adducts, considered a marker of lipoperoxidative protein damage in vivo, was evaluated in an ELISA test, completely home made and calibrated versus a concentrated pool of human plasma; this antibody titre was significantly higher in the over-90-year-old population. Plasma vitamin E, evaluated in RP-HPLC, was not significantly different between the two groups. Plasma protein-bound carbonyls, a marker of oxidative protein damage, were measured with the 2,4-dinitrophenylhydrazine assay; their level in the over-90-year-old population was lower than in the blood donors. The higher antibody titre against MDA-adducts may result in protection against accumulation of oxidatively damaged proteins by enhancing their removal, and, together with the preserved plasma vitamin E level, it may endow over-90-year-olds with an especially efficient antioxidant profile. The low level of protein carbonyl might reflect the more efficient removal of damaged proteins.
Traverso
Nicola
N
Department of Experimental Medicine, Section of General Pathology, University of Genova, Genova, Italy. ntravers@medicina.unige.it
Patriarca
Stefania
S
Balbis
Emanuela
E
Furfaro
Anna Lisa
AL
Cottalasso
Damiano
D
Pronzato
Maria Adelaide
MA
Carlier
Paolo
P
Botta
Federica
F
Marinari
Umberto Maria
UM
Fontana
Luigi
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D012372:ROC Curve; D014810:Vitamin E / Q000097:blood
D001323:Autoantibodies; D015415:Biomarkers; D001798:Blood Proteins; D014810:Vitamin E; D008315:Malondialdehyde