[Establishment of consensus sequence of PreS/S of hepatitis B virus with genotype B/serotype adw2 or genotype C/serotype adrq+ prevailing in Chongqing of China].
OBJECTIVE
To develop consensus sequence of HBV PreS/S with different subtype in Chongqing of China.


METHODS
The gene of PreS/S of HBV in 18 AsC was sequenced. The genotype and serotype of HBV were determined. The main HBV strain prevailing in Chongqing was identified to establish its consensus sequence.


RESULTS
It was found that 9 strains were genotype B/serotype adw2, 6 were genotype C/serotype adrq+ and 3 were genotype B/serotype ayw1. The consensus sequence of PreS/S of HBV with genotype B/serotype adw2 and genotype C/serotype adrq+ were established. There were 6 nucleotide variants which causing 4 amino acids change between consensus sequence of PreS/S with genotype B/serotype adw2 in Chongqing and in Southeast of China (homology showed 99.5% and 99.0% respectively). There were 13 different sites that caused 4 amino acids change between consensus sequence of PreS/S with genotype C/serotype adrq+ in Chongqing and in Northeast and South of China (homology showed 98.9% and 99.0% respectively). Comparing the two consensus sequence of PreS/S gene of different subtype HBV in Chongqing, there was 95 variants which caused 40 amino acids variants (the homology was 92.1% and 90.0% respectively).


CONCLUSIONS
The consensus sequence of PreS/S of hepatitis B virus with genotype B/serotype adw2 and genotype C/serotype adrq+ prevailing in Chongqing was established.
The Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Hong-Mei
HM
Xu
Hong
H
Ren
Yu-Ling
YL
Qing
Ming-Li
ML
Peng
Ning
N
Ling
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002648:Child; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012703:Serotyping
D006514:Hepatitis B Surface Antigens