[Cloning and expressing the E2 subunit of pyruvate dehydrogenase complex].
OBJECTIVE
To construct the expression vector of the pyruvate dehydrogenase complex E2 subunit gene (PDC-E2).


METHODS
The PDC-E2 gene was amplified from human lymphocytes with RT-PCR, and was cloned into pExSecI vector to induce the PDC-E2 expression. The products were identified with western blot and ELISA.


RESULTS
The expression vector pExSecI/PDC-E2 was successfully constructed. The products could be identified by the specific self-antibodies in the sera from the primary biliary cirrhosis patients.


CONCLUSIONS
High efficient expression vector of PDC-E2 lays the foundation for serum assay of primary biliary cirrhosis patients with prokaryotic expressing PDC-E2.
Zhao
Jun
J
Immunology Department of the Institute of Infectious Diseases, 302nd Hospital of People's Limitary Army, Beijing 100039, China.
Shu
Cui-li
CL
Lei
Li
L
Li
Jing
J
Gao
Rong
R
Cheng
Yun
Y
D003001:Cloning, Molecular; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011768:Pyruvate Dehydrogenase Complex / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D011768:Pyruvate Dehydrogenase Complex; D051047:Dihydrolipoyllysine-Residue Acetyltransferase