Microarray analysis of A549 cells infected with rabbitpox virus (RPV): a comparison of wild-type RPV and RPV deleted for the host range gene, SPI-1.
A documented consequence of poxvirus infections is global inhibition of host protein synthesis and reduction in mRNA levels. We examined this mRNA decrease by infecting A549 cells, derived from a human lung carcinoma, with rabbitpox virus (RPV), or RPV deleted for the serine protease inhibitor SPI-1 (RPVDeltaSPI-1), which exhibits a growth defect on A549 cells. At various times postinfection, mRNA profiles were analyzed using Affymetrix U95AV2 microarrays. There was a decline in overall cellular mRNA levels beginning at 2.5 hpi, and by 5 hpi, mRNA levels were drastically reduced for the majority of genes. However, several mRNAs increased, including those of heat-shock genes. Finally, a comparison of host mRNA profiles of RPV- to RPVDeltaSPI-1-infected cells revealed subtle differences in mRNA levels at 5 and 12 hpi. In summary, while there was a global decrease of host mRNA levels, the induction of selected mRNAs may be required for a successful poxvirus infection.
Department of Molecular Genetics and Microbiology and The Genetics Institute, University of Florida, Gainesville, FL 32610-0266, USA.
Lauren M
LM
Brum
M Cecilia
MC
Lopez
Juan Carlos
JC
Varela
Henry V
HV
Baker
Richard W
RW
Moyer
D000199:Actins / Q000235:genetics; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006657:Histones / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D015842:Serine Proteinase Inhibitors / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*
D000199:Actins; D018840:HSP70 Heat-Shock Proteins; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; C086597:lambda Spi-1