L-selectin and E-selectin expressed on monocytes mediating Ehrlichia chaffeensis attachment onto host cells.
Ehrlichia chaffeensis, the agent of human monocytic ehrlichiosis, is an obligatory intracellular bacterium that exhibits monocytic host cell tropism. Ehrlichiae must enter the host cell, and then establish infection. The tropism of E. chaffeensis for monocytes suggests that the cell contains some specific surface components that mediate E. chaffeensis attachment and entry into host cells. In this study, host cell surface components that play a role in ehrlichial attachment were identified using a human monocyte/macrophage cell line, THP-1. E. chaffeensis attachment to THP-1 cells was partially blocked in the presence of antibodies to E-selectin and L-selectin, but not by antibodies to P-selectin, integrin alpham, integrin alphax, or normal mouse IgG as determined by real time polymerase chain reaction. Conversely, in HeLa cells that do not exhibit surface expression of E-selectin and L-selectin, antibodies to these cell surface proteins did not inhibit E. chaffeensis attachment. These findings indicate that E-selectin and L-selectin are cell surface proteins that might act as co-receptors and contribute to E. chaffeensis attachment and entry into THP-1.
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, the University of Texas Medical Branch at Galveston, 301 Univ. Blvd., Galveston, TX 77555, USA.
Jian-zhi
JZ
Zhang
Jere W
JW
McBride
Xue-jie
XJ
Yu
D001422:Bacterial Adhesion / Q000502:physiology*; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism*; D018469:Ehrlichia chaffeensis / Q000276:immunology / Q000502:physiology*; D019041:L-Selectin / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology*; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism
D019040:E-Selectin; D011990:Receptors, Transferrin; D019041:L-Selectin