Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass.
To investigate the production of cellulases and hemicellulases from Aspergillus niger KK2, solid state fermentation (SSF) was performed by using different ratios of rice straw and wheat bran. When A. niger KK2 was grown on rice straw alone as a solid support in SSF, the maximum FPase activity was 19.5 IU g(-1) in 4 days. Also, CMCase (129 IU g(-1)), beta-glucosidase (100 IU g(-1)), xylanase (5070 IU g(-1)) and beta-xylosidase (193 IU g(-1)) activities were concurrently obtained after 5-6 days of fermentation. The higher enzyme activities produced by A. niger KK2 is a significant advantage from the viewpoint of practical saccharification reaction. Cellulases and hemicellulases produced by A. niger KK2 might be applied to pulp and paper industry, feed industry and chemical industry.
Kang
S W
SW
Graduate School of Biotechnology, Korea University, 1 Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea.
Park
Y S
YS
Lee
J S
JS
Hong
S I
SI
Kim
S W
SW
D001234:Aspergillus niger / Q000201:enzymology*; D044602:Cellulases / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism*; D004043:Dietary Fiber; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D012275:Oryza
D004043:Dietary Fiber; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D044602:Cellulases; D006026:Glycoside Hydrolases; C023305:hemicellulase