A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses.
Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.
Hong Kong DNA Chips Ltd., 1805-6, 18F, Lu Plaza, 2 Wing Yip Street, Kowloon, Hong Kong SAR, China.
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D000482:Allantois / Q000821:virology; D000818:Animals; D001483:Base Sequence; D001717:Birds; D002642:Chick Embryo / Q000821:virology; D017931:DNA Primers; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000175:diagnosis; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D021161:Self-Sustained Sequence Replication / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012367:RNA, Viral