Isolation of a polygalacturonase-inhibiting protein (PGIP) from wheat.
Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.
Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa. gabre@ccrc.uga.edu
Gabré
G
Kemp
Carl W
CW
Bergmann
Ron
R
Clay
Amie J
AJ
Van der Westhuizen
Zacharias A
ZA
Pretorius
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification / Q000187:drug effects*; D015151:Immunoblotting; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011096:Polygalacturonase / Q000037:antagonists & inhibitors*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000502:physiology*
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; C081479:PGIP protein, plant; D010940:Plant Proteins; D011096:Polygalacturonase