Dietary G-rutin suppresses glycation in tissue proteins of streptozotocin-induced diabetic rats.
The present study focused on examining the efficacy of feeding a rutin-glucose derivative (G-rutin) to inhibit glycation reactions that can occur in muscle, kidney and plasma proteins of diabetic rats. Both thiobarbituric acid-reactive substance levels and protein carbonyl contents in muscle and kidney were significantly (p < 0.05) reduced in streptozotocin-induced diabetic rats fed G-rutin supplemented diet, compared to diabetic rats fed control diet. The N(epsilon)-fructoselysine content in muscle and kidney, a biomarker of early glycation reaction, was markedly (p < 0.05) increased by diabetes, but significantly (p < 0.05) reduced in diabetic rats fed G-rutin. Advanced glycation end-products (AGEs) in serum and kidney protein were measured by immunoblot using anti-AGE antibody, and were also reduced in diabetic rats fed dietary G-rutin. Feeding G-rutin also slightly inhibited aldose reductase activity in these animals. These results demonstrate for the first time that dietary G-rutin consumption can provide potential health benefits that are related to the inhibition of tissue glycation reactions common to diabetes.
Nagasawa
Takashi
T
Food and Health Science, Faculty of Agriculture, Iwate University, Morioka, Iwate, Tokyo, Japan.
Tabata
Nobuaki
N
Ito
Yoshiaki
Y
Aiba
Youichi
Y
Nishizawa
Naoyuki
N
Kitts
David D
DD
D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004032:Diet; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000008:administration & dosage / Q000494:pharmacology*; D013311:Streptozocin
D017127:Glycation End Products, Advanced; C033186:fructosyl-lysine; D012431:Rutin; D013311:Streptozocin; D005947:Glucose; D008239:Lysine