Expression of mRNA for interleukin-1beta, interleukin-6, tumor necrosis factor-alpha and macrophage migration inhibitory factor in HPA-axis tissues in Schistosoma mansoni-infected baboons (Papio cynocephalus).
Cytokines may regulate the function of the hypothalamic-pituitary-adrenal axis during schistosomiasis. This possibility was investigated in baboons experimentally infected with Schistosoma mansoni. Serum levels of corticotrophin-releasing hormone, adrenocorticotrophin, cortisol and dehydroepiandrosterone were confirmed to be decreased in infected baboons as previously shown. To explore if this effect is associated with specific expression of cytokines with endocrine activity, and are also associated with the pathology of the disease, Northern blots for interleukin-1beta, interleukin-6, tumor necrosis factor-alpha and macrophage migration inhibitory factor in hypothalamic-pituitary-adrenal axis tissues were performed. Infection induced interleukin-1beta gene expression in the hypothalamus, while interleukin-6 and migration inhibitory factor mRNAs were induced only in the pituitary and adrenal glands. Tumor necrosis factor-alpha gene expression was induced in the hypothalamus and the pituitary gland. Histopathological analysis of the hypothalamic-pituitary-adrenal axis tissues in infected and control baboons revealed no morphological differences between them. These results suggest that specific cytokines expressed in hypothalamic-pituitary-adrenal axis tissues could regulate hormone secretion during schistosomiasis.
Morales-Montor
Jorge
J
Departamento de Inmunologi;a, Instituto de Investigaciones Biomédicas, UNAM AP 70228, Mexico DF 04510, Mexico. jmontor66@hotmail.com
Mohamed
Fawzi
F
Baghdadi
Amjed
A
Baig
Salman
S
Hallal-Calleros
Claudia
C
Damian
Raymond T
RT
D000311:Adrenal Glands / Q000276:immunology; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D002908:Chronic Disease; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood; D005260:Female; D015870:Gene Expression; D006854:Hydrocortisone / Q000097:blood; D007031:Hypothalamus / Q000276:immunology; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007411:Intestinal Diseases, Parasitic / Q000097:blood / Q000276:immunology* / Q000662:veterinary*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics; D008297:Male; D008992:Monkey Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology*; D010215:Papio; D010902:Pituitary Gland / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012555:Schistosomiasis mansoni / Q000097:blood / Q000276:immunology* / Q000662:veterinary*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D007375:Interleukin-1; D015850:Interleukin-6; D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019314:Dehydroepiandrosterone Sulfate; D006854:Hydrocortisone