Right ventricle of patients undergoing congenital cardiac surgery differentially expresses haem oxygenase-1 and heat shock protein 70 genes.
Reactive oxygen species are implicated in the pathogenesis of cardiac hypertrophy. Haem oxygenase-1 (HO-1), the rate-limiting enzyme in haem catabolism, is induced by oxidative stress and confers protection against oxidative tissue injuries. We used Northern blotting to examine expression of HO-1 and heat shock protein 70 (HSP70) in the hypertrophic cardiac muscle of eight patients (one infant and seven children) who underwent surgery for congenital heart disease. Levels of HO-1 and HSP70 mRNA were significantly increased in all specimens, but the orders of magnitude of the increases were different, suggesting that the genes expressing HO-1 and HSP70 are regulated separately.
Department of Anaesthesiology and Resuscitology, Okayama University Medical School, Japan.
M
M
Takeuchi
T
T
Takahashi
N
N
Taga
T
T
Iwasaki
K
K
Ohe
H
H
Shimizu
T
T
Suzuki
H
H
Nakatsuka
M
M
Yokoyama
S
S
Sano
R
R
Akagi
K
K
Morita
D000975:Antioxidants / Q000378:metabolism; D015152:Blotting, Northern; D002675:Child, Preschool; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006331:Heart Diseases / Q000151:congenital*; D006352:Heart Ventricles / Q000201:enzymology*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006801:Humans; D007223:Infant; D008297:Male; D008565:Membrane Proteins; D018384:Oxidative Stress
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1