Gel and polymer-solution mediated separation of biopolymers by capillary electrophoresis.
In the age of genomics and proteomics, high-resolution separation techniques are routinely utilized in an integrated and automated fashion to solve formidable separation problems and provide the means for large-scale analysis of biological samples with excellent resolution. By automating the current manual procedures, capillary gel (CGE) and polymer-solution mediated electrophoresis greatly enhance the productivity of biopolymer analysis while also reducing both analysis time and the human intervention necessary from sample loading to data processing. The advent of this novel and high-performance bioseparation technique has made it possible to sequence the human genome and revealed global changes in the genome and proteome level, bringing about a revolutionary transition in our views of living systems on the molecular basis. CGE and polymer-solution mediated electrophoresis and related microseparation methods (e.g., electrophoresis microchips) are quickly becoming important separation and characterization tools in analytical biochemistry and molecular biology. This review gives an overview of the key application areas of DNA, protein, and complex carbohydrate analysis, and summarizes the latest developments on CGE column technology, including capillary coatings and sieving polymer matrices. Micropreparative aspects and related microseparation techniques are also discussed.
Torrey Mesa Research Institute, 3115 Merryfield Row, La Jolla, CA 92121, USA.
András
A
Guttman
D001704:Biopolymers / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D020543:Proteome
D001704:Biopolymers; D020543:Proteome