Effect of moderate acute exercise on expression of mRNA involved in the calcineurin signaling pathway in human skeletal muscle.
Calcineurin, a calcium-regulated protein phosphatase, activates gene expression specific to slow muscle fibers by dephosphorylating a family of the nuclear factor of activated T cells (NFAT), which cooperates with myocyte enhancer factor-2 (MEF2) and AP-1. However, it remains unknown how acute exercise influences this signaling pathway and leads to the development of slow muscle fibers. In the present study, we investigated the effect of moderate acute exercise on mRNA expression of genes in the calcineurin signaling pathway in human skeletal muscle. Five healthy volunteers underwent 1 h bicycle ergometer at 50%VO2max, and vastus lateralis muscle biopsies were collected before and after exercise. Four hours after exercise, alterations in mRNA expression of NFAT 1-3 were observed with a wide variety among subjects, while c-fos mRNA was significantly induced in all subjects. By contrast, the expression of calcineurin, MEF2, and myocyte-enriched calcineurin-interacting protein 1 (MCIP1) remained unchanged. These results suggest that even moderate acute exercise may change mRNA expression of genes in the calcineurin-signaling pathway.
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan. yhitomi@kyorin-u.ac.jp
Yoshiaki
Y
Hitomi
Takako
T
Kizaki
Toshihito
T
Katsumura
Masao
M
Mizuno
Choh-ei
CE
Itoh
Kazuki
K
Esaki
Yasunori
Y
Fujioka
Tohru
T
Takemasa
Shukoh
S
Haga
Hideki
H
Ohno
D019703:Calcineurin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015444:Exercise; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D064326:MEF2 Transcription Factors; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology*; D009132:Muscles / Q000166:cytology / Q000473:pathology; D018006:Myogenic Regulatory Factors; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D010100:Oxygen / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D064326:MEF2 Transcription Factors; D009124:Muscle Proteins; D018006:Myogenic Regulatory Factors; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D016760:Proto-Oncogene Proteins c-fos; C108612:RCAN1 protein, human; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D012313:RNA; D019703:Calcineurin; D010100:Oxygen