[Effects of langchuangjing granule on apoptotic of CD4+ T and CD19+ B in spleen of BXSB mice with systemic lupus erythematosus].
OBJECTIVE
To study the effect on apoptotic of CD4+ T, CD19+ B in spleen of BXSB mice with systemic lupus erythematosus treated with Langchuangjing Granule and to probe into the mechanism of treatment.


METHODS
The apoptosis was examined by the flow cytometric analysis and immunofluorescence double-staining method.


RESULTS
Apoptosis of male BXSB mice speeds up. Langchuangjing Granule can restrain the excessive apoptosis of CD4+ T and CD19+ B cells in spleen.


CONCLUSIONS
Langchuangjing Granule treated systemic lupus erythematosus by restraining the excessive apoptotic of T, B lymphocytes, probably restraining the release of excessive amount of apoptotic DNA fragments, so decreasing abnormal proliferation of B cells and the produce of autoantibodies.
Department of Rheumatology and Immunology, Zhejiang Provincial Hospital of the Integrative Chinese and Western Medicine, 310003. Liuxide2001@shou.com
Xi-de
XD
Liu
Shi
S
Jin
D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D013154:Spleen / Q000473:pathology
D018941:Antigens, CD19; D004365:Drugs, Chinese Herbal