Peripheral and central proinflammatory cytokine response to a severe acute stressor.
The role of proinflammatory cytokines in the response to acute stressor exposure has received recent attention. Exposure to a single session of inescapable shock (IS) induces peripheral and central proinflammatory cytokines. Other stressors also increase expression of proinflammatory cytokine mRNA and/or protein in various tissues. However, the induction of central and peripheral proinflammatory cytokines by stressors remains controversial and the pattern of cytokine induction is not consistent across stressors. The present experiments sought to examine the pattern of the proinflammatory cytokine response to a stressor known to cause elevations of IL-1beta protein. mRNA expression for three proinflammatory cytokines, IL-1beta, TNF-alpha and IL-6, and IL-1beta protein was examined after IS. IS increases IL-1beta mRNA and/or protein in a variety of tissues, including hypothalamus, hippocampus, pituitary and spleen. Furthermore, IS concomitantly alters IL-1beta mRNA and protein in hypothalamus and spleen, while the IL-1beta mRNA increase in pituitary lags behind the increase of IL-1beta protein. Interestingly, IL-1beta mRNA is elevated in hippocampus 4 h after IS, but an increase of IL-1beta protein in hippocampus is not detected. Expression of TNF-alpha and IL-6 mRNA do not increase in response to IS. Indeed, TNF-alpha mRNA expression decreases in cortex, pituitary and liver immediately after IS. These findings suggest that alterations of proinflammatory cytokine expression by stressors, and IS in particular, are region- and cytokine-specific.
O'Connor
Kevin A
KA
Department of Psychology and Center for Neuroscience, Campus Box 345, University of Colorado at Boulder, Boulder, CO 80309-0345, USA. koconnor@psych.colorado.edu
Johnson
John D
JD
Hansen
Michael K
MK
Wieseler Frank
Julie L
JL
Maksimova
Elena
E
Watkins
Linda R
LR
Maier
Steven F
SF
D000818:Animals; D001921:Brain / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004597:Electroshock; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D007375:Interleukin-1 / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D008297:Male; D017933:Peripheral Nervous System / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013315:Stress, Psychological / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha