[Organization and differential staining of chromosomes from the endomitotic nucleus of trophocytes Calliphora erythrocephala (Diptera: Calliphoridae)].
The structure of primary polytene chromosomes and general architecture of nurse cell nuclei was studied in Calliphora erythrocephala using various methods of differential chromosome banding(G-, R-, C-banding; Ag- and DAPI staining), chromospecific DNA probes and fluorescence in situ hybridization. This analysis revealed differential compaction of particular chromosome regions. The localization of material of polytene chromosome 6 is retained after its rearrangement and the formation of the internal reticular structure of the nucleus. Polytene chromosomes of ovarian nurse cells were shown to have blocks of dense compact material; some of them were more intensely stained by AgNO3. The dynamics of the nucleolus formation was traces at all stages of chromosome polytenization in the C. erythrocephala nurse cells.
Vasserlauf
I E
IE
Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050 Russia.
Anan'ina
T V
TV
Unger
M F
MF
Karamysheva
T V
TV
Mel'nikova
N N
NN
Rubtsov
N B
NB
Stegniĭ
V N
VN
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D020223:Chromosome Painting; D015342:DNA Probes; D004175:Diptera / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis
D015342:DNA Probes