Sodium pyruvate protects against H(2)O(2) mediated apoptosis in human neuroblastoma cell line-SK-N-MC.
Free radicals are involved in neuronal damage. The present study was aimed to investigate the protective effect of sodium pyruvate-a free radical scavenger against hydrogen peroxide (H(2)O(2)) induced apoptosis in human neuroblastoma cell line-SK-N-MC. On exposure to H(2)O(2) (0.025 mM) cells exhibited apoptosis within 24 h, demonstrating a high caspase 3 activity by 3 h followed by cleavage of PARP that was maximum at 24 h. A break down in the mitochondrial membrane potential was observed 3 h onwards. Sodium pyruvate protected cells significantly (P<0.05) against apoptosis in a dose dependent manner as assessed for cell viability by dye exclusion method and apoptosis by TUNEL. Sodium pyruvate significantly inhibited caspase 3 activity, cleavage of PARP and breakdown of mitochondrial membrane potential. These data suggest that sodium pyruvate protects neuronal damage caused by H(2)O(2).
Department of Biotechnology, National Center for Cell Science, Government of India An Autonomous Institution, Pune University Campus, Ganeshkhind, 411007 Pune, India.
Jayashree C
JC
Jagtap
Anmol
A
Chandele
B A
BA
Chopde
Padma
P
Shastry
D000975:Antioxidants; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002470:Cell Survival; D003124:Colorimetry; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D008564:Membrane Potentials / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology*; D018696:Neuroprotective Agents; D016877:Oxidants / Q000633:toxicity*; D019289:Pyruvic Acid / Q000494:pharmacology*
D000975:Antioxidants; D018696:Neuroprotective Agents; D016877:Oxidants; D019289:Pyruvic Acid; D006861:Hydrogen Peroxide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases