Preparative separation of rhein from Chinese traditional herb by repeated high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was repeatedly used for isolation and purification of rhein from Rheum officinale Baill (Dahuang) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:7:5:5, v/v), which had been selected by analytical (HSCCC). Using two preparative units of the HSCCC centrifuge, about a 500 mg amount of the crude extract was separated, yielding 6.7 mg of rhein at a high purity of over 97%.
Wei
Yun
Y
Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology Application, Beijing 100035, PR China.
Zhang
Tianyou
T
Ito
Yoichiro
Y
D000880:Anthraquinones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; C020491:rhein