[Changes in hydrodynamic diameter of myometrium plasmalemma vesicles under artificial creation of transmembrane proton gradients].
A method of laser-correlation spectrophotometry was applied for finding the effect of proton transmembrane gradient on hydrodynamic diameter of plasmalemma smooth muscle vesicles. It is shown, that the building of protons gradient on the membrane of vesicles, pHi = 6.0; pHo = 7.5 (delta pH = 1.5) results in the reliable decrease of their hydrodynamic diameter (from 212 +/- 6 nm in monitoring to 174 +/- +/- 0.25 nm in experiment). The exit of K+ from vesicles (KCl concentration inside vesicles is 150 mM), on the contrary, evokes augmentation of hydrodynamic diameter. The chemical paravariation of COO-, NH(3+)-groups and stabilization of SH-groups of plasmalemma surface results in essential modifications in magnitude of the hydrodynamic diameter of vesicles. On the basis of experimental data a supposition is expressed about the role of modifications of regional position of the functionally important groups of plasmalemma with respect to the membrane surface in mechanisms of H+ transport.
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Iu V
IuV
Danylovych
A V
AV
Kliuieva
Iu M
IuM
Levchuk
V A
VA
Tuhaĭ
D000818:Animals; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D009215:Myometrium / Q000166:cytology; D011188:Potassium / Q000378:metabolism; D011522:Protons; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013053:Spectrophotometry; D013552:Swine; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
D011522:Protons; D004024:Dicyclohexylcarbodiimide; D014302:Trinitrobenzenesulfonic Acid; D011188:Potassium; D004229:Dithiothreitol