Microcosm tributyltin bioaccumulation and multibiomarker assessment in the blue mussel Mytilus edulis.
Blue mussels, Mytilus edulis, were exposed to two different concentrations of tributyltin (TBT) in seawater, 1,000 ng Sn/L (C1 experiment) and 10 ng Sn/L (C2 experiment), for 4 d, in order to evaluate the bioaccumulation of TBT by mussels Mytilus edulis in microcosms and to test the ability of a multimarkers analysis to determine the effects of TBT on the biochemical parameters in mussels. Tissue burdens of Mytilus edulis were 204 +/- 7 and 2,120 +/- 4 ng Sn/g TBT after the 4-d tests for the C2 and C1 experiments, respectively. Analyses of dissected organs and/or tissues demonstrated that TBT accumulated to the greatest extent in gills in the C1 experiment and in the digestive gland in the C2 experiment. Bioconcentration factors (BCFs) were 12,100 +/- 300 and 2,000 +/- 10 for mussels exposed in the C2 and C1 experiments, respectively. The four biomarkers used in this work were acetylcholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT) activities, and thiobarbituric acid-reactive substances (TBARS) contents. No significant changes were observed in the measured enzyme activities or in TBARS concentration after the 4-d TBT exposure.
Laboratoire de Physico- et Toxicochimie des Systèmes Naturels, UMR 5472 CNRS, Université de Bordeaux 1, 351 Cours de la Libération, 33405 Talence Cedex, France.
Marie-Hélène
MH
Devier
Sylvie
S
Augagneur
Hélène
H
Budzinski
Pascal
P
Mora
Jean-François
JF
Narbonne
Philippe
P
Garrigues
D000110:Acetylcholinesterase / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D049872:Bivalvia / Q000502:physiology*; D002374:Catalase / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014018:Tissue Distribution; D014220:Trialkyltin Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D014873:Water Pollutants / Q000493:pharmacokinetics* / Q000633:toxicity*
D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D014220:Trialkyltin Compounds; D014873:Water Pollutants; C011559:tributyltin; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase