Effects of heavy metals on population growth and metallothionein gene expression in the mosquito Culex quinquefasciatus, from Calcutta, India.
Major water bodies in and around the city of Calcutta (India) receive heavy metal contaminated effluents from industries, households, and vehicular traffic through sewage or drainage. We quantified concentrations of Cu, Zn, and Cd from three water bodies at Kalighat, Tangra, and VIP Road, respectively. The concentrations of these heavy metals were significantly greater in the summer than in monsoon when heavy downpours resulted in reduced metal concentrations. Concentrations of metals in the mosquito Culex quinquefasciatus also reflected such seasonal fluctuations. Hatchability and survivorship of C. quinquefasciatus significantly differed among the sites and were reduced significantly from the control. Exposure to heavy metals also induced MT-gene expression in C. quinquefasciatus, likely helping them to survive in the water bodies stressed with heavy metals. MT-gene activity demonstrated significant variation among sites and seasons with the highest activity in the summer in the VIP Road population. This study suggests that C. quinquefasciatus could be used as an ecological indicator of heavy metal pollution by monitoring its MT-gene expression.
Sarkar
Sagartirtha
S
Genetics Laboratory, Department of Zoology, Calcutta University, Calcutta, India.
Duttagupta
Asish K
AK
Mal
Tarun K
TK
D000818:Animals; D003465:Culex / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva / Q000737:chemistry; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D012621:Seasons; D015996:Survival Rate; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D019216:Metals, Heavy; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D008668:Metallothionein