Rapid fibroblast adhesion to 27nm high polymer demixed nano-topography.
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, G12 8QQ, UK. m.dalby@bio.gla.ac.uk
M J
MJ
Dalby
D
D
Giannaras
M O
MO
Riehle
N
N
Gadegaard
S
S
Affrossman
A S G
AS
Curtis
D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D013343:Styrenes / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D020744:rac GTP-Binding Proteins / Q000378:metabolism
D001672:Biocompatible Materials; D045424:Complex Mixtures; D008567:Membranes, Artificial; D011108:Polymers; D011137:Polystyrenes; D013343:Styrenes; C018238:beta-bromostyrene; D020744:rac GTP-Binding Proteins