Cellular uptake of a dexamethasone palmitate-low density lipoprotein complex by macrophages and foam cells.
To evaluate the utility of a dexamethasone palmitate (DP)-low density lipoprotein (LDL) complex to transport drug into foam cells, the cellular uptake of DP-LDL complex by macrophages and foam cells was examined. The DP-LDL complex was prepared by incubation with DP and LDL, and the DP-LDL complex and murine macrophages were incubated. No cellular uptake of the DP-LDL complex by macrophages was found until 6 h after the start of incubation, but this gradually increased from 12 to 48 h. On the other hand, the cellular uptake of the oxidized DP-LDL complex was already apparent at 3 h after the start incubation, and then markedly increased until 48 h incubation along with that of the lipid emulsion (LE) containing DP (DP-LE). The cellular uptake of DP-LE by foam cells was significantly lower than that by macrophages. However, the cellular uptake of DP-LDL complex by foam cells was similar to that by macrophages. These findings suggest that the DP-LDL complex is oxidatively modified, and then incorporated into macrophages and foam cells through the scavenger receptor pathway. Since selective delivery of drugs into foam cells in the early stage of atherosclerosis is a useful protocol for antiatherosclerosis treatment, the DP-LDL complex appears to be a potentially useful drug-carrier complex for future antiatherosclerotic therapy.
Tauchi
Yoshihiko
Y
Department of Pharmaceutics, Hokkaido College of Pharmacy, Otaru-city, Japan. ytmickey@ybb.ne.jp
Chono
Sumio
S
Morimoto
Kazuhiro
K
D000818:Animals; D001693:Biological Transport, Active; D002478:Cells, Cultured; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D004337:Drug Carriers; D004655:Emulsions; D005487:Foam Cells / Q000378:metabolism*; D008077:Lipoproteins, LDL; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction; D013997:Time Factors
D004337:Drug Carriers; D004655:Emulsions; D008077:Lipoproteins, LDL; C035081:dexamethasone 21-palmitate; D003907:Dexamethasone