Fast analysis of nicotine related alkaloids in tobacco and cigarette smoke by megabore capillary gas chromatography.
A novel fast megabore capillary gas chromatographic (MCGC) method for analysis of 7 nicotine related alkaloids in tobacco and cigarette smoke, including nicotine, nornicotine, myosmine, nicotyrine, anabasine, anatabine and 2,3-dipyridyl, was developed. The use of megabore capillary column GC methodology, equipped with flame ionization detector (FID), provided rapid, unambiguous nicotine related alkaloids analysis. One gram flue-cured tobacco (or Cambridge filter pad), 20 ml ether, and 5 ml 10% sodium hydroxide solution, added with n-heptadecane as the internal standard, were placed in a flask, and the flask was capped and placed in an ultrasonic bath for 15 min. A 1 microl volume was analyzed by capillary GC operating in split-injection mode on a mega bore Simplicity-5 column. This simple procedure was compared with the previously reported packed column GC method and the Griffith still-colorimetric method. The application of the method for analysis of various flue-cured tobaccos and cigarette smoke was discussed.
Department of Chemistry, University of Science & Technology of China, Hefei 230026, China. jbcai@ustc.edu.cn
Jibao
J
Cai
Baizhan
B
Liu
Ping
P
Lin
Qingde
Q
Su
D000470:Alkaloids / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D009538:Nicotine / Q000032:analysis*; D012906:Smoke / Q000032:analysis*
D000470:Alkaloids; D012906:Smoke; D009538:Nicotine