Covalent immobilization of chitosan and heparin on PLGA surface.
Chitosan and heparin were covalently immobilized onto a poly(lactic acid-co-glycolic acid) (PLGA) surface using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS) in a 2-morpholinoethane sulfonic acid (MES) buffer system. The properties of the modified PLGA surface and the control were investigated by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). The water contact angle of the modified film was greatly decreased and the element content on the surface of the films changed correspondingly. Platelet adhesion assay showed that blood compatibility of the chitosan/heparin modified film was improved while hepatocyte culture indicated that the cell compatibility of the modified film was enhanced.
Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, PR China. wangxiaohong@tsinghua.edu.cn
X H
XH
Wang
D P
DP
Li
W J
WJ
Wang
Q L
QL
Feng
F Z
FZ
Cui
Y X
YX
Xu
X H
XH
Song
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D006493:Heparin / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D010973:Platelet Adhesiveness; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
D001672:Biocompatible Materials; D011108:Polymers; D002686:Chitin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006493:Heparin; D048271:Chitosan