Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus.
Epstein-Barr virus (EBV) infection is a multi-step process, first requiring virus binding to the host cell, followed by fusion of the viral envelope with the host cell plasma membrane. Efficient EBV entry into B cells requires, at the minimum, the interaction of the EBV-encoded glycoproteins gp350 with cellular CD21 and gp42 with MHC class II proteins. In this study, use of the cholesterol-binding drugs methyl-beta-cyclodextrin and nystatin efficiently inhibited EBV infection of target Burkitt's lymphoma B-cell lines, indicating an important role for cholesterol and suggesting the involvement of lipid rafts in EBV infection.
Katzman
Rebecca B
RB
Department of Microbiology-Immunology, Northwestern University Medical School Chicago, IL 60611, USA.
Longnecker
Richard
R
Department of Microbiology-Immunology, Northwestern University Medical School Chicago, IL 60611, USA.
D002051:Burkitt Lymphoma / Q000821:virology*; D002784:Cholesterol / Q000502:physiology*; D003505:Cyclodextrins / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology; D021962:Membrane Microdomains / Q000502:physiology; D009761:Nystatin / Q000494:pharmacology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000502:physiology; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D009761:Nystatin; D002784:Cholesterol