Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples.
This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody-antigen complexes were separated from free Fc-conjugated IgG antibody (Fc-IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200-2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.
Lim
Tae-Kyu
TK
Department of Biotechnology and Life Sciences, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
Ohta
Hiroko
H
Matsunaga
Tadashi
T
D004563:Electrochemistry / Q000295:instrumentation*; D005296:Ferrous Compounds / Q000737:chemistry; D006632:Histamine / Q000097:blood*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis; D000075163:Metallocenes; D008838:Microcomputers; D036103:Nanotechnology; D011058:Pollen / Q000276:immunology; D019904:Polymethyl Methacrylate
D005296:Ferrous Compounds; D007074:Immunoglobulin G; D000075163:Metallocenes; D006632:Histamine; D019904:Polymethyl Methacrylate; C004998:ferrocene