Is elective abdominal aortic aneurysm repair accompanied by high grade oxidative stress?
OBJECTIVE
During elective abdominal aortic aneurysm repair (AAAR), lower torso ischaemia-reperfusion event is unavoidable. Previous studies on AAAR have reported the importance of oxidative stress (OS) in ischaemia-reperfusion injury, however, the grade of OS has not been adequately clarified up to now. The aim of this study was to perform a complex investigation of the time-course and grade of systemic and cellular OS in patients undergoing AAAR.


METHODS
Arterial blood samples were taken from 18 patients undergoing elective AAAR (at four points in time: before anaesthesia, 5 min after aortic clamping and 5 min and 30 min after clamp removal). Diene conjugates (DC), thiobarbituric acid reactive substances (TBARS), total antioxidative capacity (TAC), glutathione redox ratio (GSSG/GSH), and levels of antioxidative enzymes as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) were measured from the radial arterial blood.


RESULTS
30 min after the removal of the aortic cross-clamp, arterial CAT showed significant elevation (96.0 vs 56.9 U/l, p < 0.05); GSHPx was significantly elevated (51.5 vs 39.9 U/g Hgb, p < 0.05) and TAC was decreased (31.4 vs 36.5%, p < 0.05) in comparison with preoperative value.


CONCLUSIONS
We found limited alterations of several OS parameters, which do not characterize either systemic or cellular high-grade OS during elective AAAR.
Department of Vascular and Thoracic Surgery, University of Tartu, Tartu, Estonia. Priit.Poder@kliinikum.ee
P
P
Põder
A
A
Pulges
J
J
Kals
A
A
Aavik
K
K
Zilmer
T
T
Kullisaar
C
C
Kairane
M
M
Zilmer
D000368:Aged; D017544:Aortic Aneurysm, Abdominal / Q000097:blood* / Q000201:enzymology / Q000601:surgery*; D002374:Catalase / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase