G484S amino acid substitution in lanosterol 14-alpha demethylase (ERG11) is related to fluconazole resistance in a recurrent Cryptococcus neoformans clinical isolate.
Five sequential Cryptococcus neoformans isolates recovered from an AIDS patient with recurrent meningitis were analyzed. Four isolates were fluconazole susceptible, while the fifth isolate developed fluconazole resistance. Analysis of the 14-alpha lanosterol demethylase gene (ERG11) showed a point mutation in the resistant strain responsible for the amino acid substitution G484S.
Rodero
Laura
L
Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Hospital J. A. Fernandez, Buenos Aires, Argentina. lrodero@anlis.gov.ar
Mellado
Emilia
E
Rodriguez
A Carolina
AC
Salve
Angela
A
Guelfand
Liliana
L
Cahn
Pedro
P
Cuenca-Estrella
Manuel
M
Davel
Graciela
G
Rodriguez-Tudela
Juan L
JL
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000328:Adult; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D008297:Male; D016919:Meningitis, Cryptococcal / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D017354:Point Mutation / Q000235:genetics; D012008:Recurrence; D058886:Sterol 14-Demethylase
D000935:Antifungal Agents; C548418:CYP51A1 protein, human; D015725:Fluconazole; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase