A novel urea sensitive biosensor with extended dynamic range based on recombinant urease and ISFETs.
A novel urea biosensor based on immobilised recombinant urease as sensitive element and ion sensitive field effect transistor as transducer was developed. Recombinant urease from E. coli with an increased Km was photoimmobilised in PVA/SbQ (poly(vinyl alcohol) containing styrylpyridinium) membrane and has demonstrated quite good performance as biosensitive element. Enzymatic field effect transistors based on such a bioselective element were studied in model buffer solutions. This biosensor demonstrated an extended dynamic range up to 80 mM, a quite good reproducibility (standard deviation of the sensor responses was approximately 2.5%, n= 20 for urea concentration 10 mM) and a high stability. Such characteristics fit with the analytical requirements needed for urea control in plasma and liquids used during renal dialysis.
Soldatkin
A P
AP
Ecole Centrale de Lyon, UMR/CNRS 5621, 36 Av. Guy de Collongue, F69134 Ecully, France. a_soldatkin@yahoo.com
Montoriol
J
J
Sant
W
W
Martelet
C
C
Jaffrezic-Renault
N
N
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic; D014508:Urea / Q000032:analysis* / Q000737:chemistry*; D014510:Urease / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics
D004800:Enzymes, Immobilized; D011994:Recombinant Proteins; D014508:Urea; D014510:Urease