High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging.
Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and a femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in a new operating regime in which a short coherence gate is used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular imaging is demonstrated in the Xenopus laevis tadpole and in human skin with a 3-microm coherence gate and a 30-microm confocal gate. The ability to achieve cellular imaging with a lower numerical aperture should facilitate the development of miniaturized probes for in vivo imaging applications.
Aguirre
A D
AD
Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Hsiung
P
P
Ko
T H
TH
Hartl
I
I
Fujimoto
J G
JG
D000818:Animals; D002477:Cells / Q000166:cytology*; D004867:Equipment Design; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation; D012867:Skin / Q000166:cytology; D041623:Tomography, Optical Coherence / Q000295:instrumentation; D014982:Xenopus laevis