Exon-1 polymorphism of ctla-4 gene in Iranian patients with Graves' disease.
Polymorphisms in ctla-4 gene have been shown to be associated with the Graves' disease (GD) susceptibility in different populations in the world. This study was undertaken to disclose the probable association of exon-1 polymorphism of ctla-4 with GD in Iranian patients. A49G polymorphism was investigated in 90 patients and 90 age/sex matched normal healthy controls, using PCR-SSCP and PCR-RFLP methods. Frequencies of AA, AG and GG genotypes among patients were found to be 21 (23.3%), 49 (54.5%) and 20 (22.2%) while these frequencies among healthy controls were 30 (33.3%), 53 (58.9%) and 7(7.8%), respectively. A significant increase of GG genotype and G allele was observed in patients (p = 0.012 and p = 0.025). In conclusion, consistent with the results of most other studies, the presence of a G allele in position 49 of ctla-4 exon-1 is associated with susceptibility to GD in Iranian population.
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
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T
Kalantari
H
H
Mostafavi
A M
AM
Pezeshki
S
S
Farjadian
M
M
Doroudchi
F
F
Yeganeh
A
A
Ghaderi
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology*; D060908:CTLA-4 Antigen; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005787:Gene Frequency; D006111:Graves Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D004247:DNA