Molecular epidemiological study of Brachyspira pilosicoli in Finnish sow herds.
Brachyspira (B.) pilosicoli, the causative agent of intestinal spirochaetosis in pigs, is a quite common laboratory finding from faecal samples of weaned and growing pigs in Finland. A better understanding of the epidemiology of B. pilosicoli in and between Finnish pig farms is needed. Altogether 131 B. pilosicoli isolates from 49 Finnish sow herds were studied by pulsed-field gel electrophoresis. MluI was used as a restriction enzyme for all the isolates, and SmaI for 70 isolates. The isolates were divided into 54 different macrorestriction profiles (MRP) by MluI. Most farms had distinct B. pilosicoli genotypes, and common genotypes among herds were rare. B. pilosicoli was re-isolated after 3 years in three herds; the same MRP persisted in each of these herds. A genetic clustering of B. pilosicoli isolates between two major pork production areas was not detected.
Fossii
M
M
National Veterinary and Food Research Institute, Seinäjoki Regional Unit POB 198, 60101 Seinäjoki, Finland.
Pohjanvirta
T
T
Pelkonen
S
S
D000818:Animals; D017400:Brachyspira / Q000302:isolation & purification*; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005260:Female; D005387:Finland / Q000453:epidemiology; D017720:Molecular Epidemiology; D013145:Spirochaetales Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*