Bioluminescent bioreporter integrated-circuit sensing of microbial volatile organic compounds.
A bioluminescent bioreporter for the detection of the microbial volatile organic compound p-cymene was constructed as a model sensor for the detection of metabolic by-products indicative of microbial growth. The bioreporter, designated Pseudomonas putida UT93, contains a Vibrio fischeri luxCDABE gene fused to a p-cymene/p-cumate-inducible promoter derived from the P. putida F1 cym operon. Exposure of strain UT93 to 0.02-850 ppm p-cymene produced self-generated bioluminescence in less than 1.5 h. Signals in response to specific volatile organic compounds (VOCs) such as m- and p-xylene and styrene, also occurred, but at two-fold lower bioluminescent levels. The bioreporter was interfaced with an integrated-circuit microluminometer to create a miniaturized hybrid sensor for remote monitoring of p-cymene signatures. This bioluminescent bioreporter integrated-circuit device was capable of detecting fungal presence within approximately 3.5 h of initial exposure to a culture of p-cymene-producing Penicillium roqueforti.
Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, Knoxville, TN 37996-1605, USA.
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S
Ripp
K A
KA
Daumer
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T
McKnight
L H
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Levine
J L
JL
Garland
M L
ML
Simpson
G S
GS
Sayler
D000391:Air Microbiology; D001709:Biotechnology / Q000379:methods*; D000081007:Cymenes; D004784:Environmental Monitoring / Q000379:methods; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics*; D039821:Monoterpenes / Q000378:metabolism*; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism; D018877:Sick Building Syndrome / Q000382:microbiology*; D020058:Styrene / Q000378:metabolism; D014733:Vibrio / Q000235:genetics; D014992:Xylenes / Q000378:metabolism
D000081007:Cymenes; D008164:Luminescent Proteins; D039821:Monoterpenes; D014992:Xylenes; C007210:4-cymene; D020058:Styrene; C031286:4-xylene; C031285:3-xylene