Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.
Extracts from poliovirus-infected HeLa cells were used to study ribosome binding of native and denatured reovirus mRNAs and translation of capped mRNAs with different degrees of secondary structure. Here, we demonstrate that ribosomes in extracts from poliovirus-infected cells could form initiation complexes with denatured reovirus mRNA, in contrast to their inability to bind native reovirus mRNA. Furthermore, the capped alfalfa mosaic virus 4 RNA, which is most probably devoid of stable secondary structure at its 5' end, could be translated at much higher efficiency than could other capped mRNAs in extracts from poliovirus-infected cells.
Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1Y6.
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Sonenberg
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Guertin
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Lee
D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000737:chemistry / Q000378:metabolism*
D012315:RNA Caps