Calcium restores prepenetration morphogenesis abolished by polyamines in Colletotrichum gloeosporioides infecting red pepper.
Colletotrichum gloeosporioides forms a specialized infection structure, an appressorium, to infect its host, red pepper. Polyamines (putrescine, spermidine, and spermine) as well as S-adenosyl methionine inhibitor, methylglyoxal-bis-guanyl hydrazone (MGBG), impaired conidial germination and appressorium formation of C. gloeosporioides. Curtailment of cell differentiation by polyamines and MGBG was more evident in conidial germination than in appressorium development. Exogenous addition of calcium restored conidial germination and appressorium formation and expression of calmodulin-encoding gene (CgCaM) inhibited by polyamines. Taken together, proper regulation of intracellular polyamine concentration is indispensable for conidial germination and appressorium formation, and involved in Ca(2+)/calmodulin-dependent signaling pathways of C. gloeosporioides infecting red pepper.
School of Agricultural Biotechnology, Seoul National University, 441-744, Suwon, South Korea.
Il-Pyung
IP
Ahn
Soonok
S
Kim
Woo-Bong
WB
Choi
Yong-Hwan
YH
Lee
D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000235:genetics / Q000502:physiology*; D002212:Capsicum / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D020231:Colletotrichum / Q000187:drug effects* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D009024:Morphogenesis; D010935:Plant Diseases / Q000382:microbiology; D011073:Polyamines / Q000494:pharmacology*
D002147:Calmodulin; D011073:Polyamines; D002118:Calcium