Collection of selected reaction monitoring and full scan data on a time scale suitable for target compound quantitative analysis by liquid chromatography/tandem mass spectrometry.
A hybrid linear ion trap/triple quadrupole mass spectrometer was used to demonstrate the value of collecting full scan qualitative data during quantitative analysis of target compounds. We present examples of the additional information that can be obtained from plasma samples analyzed primarily for target compound concentrations. This information includes detection of circulating metabolites, dosing vehicle, interfering matrix components, and potential interfering drug conjugates. Additionally, the quantitative results from selected reaction monitoring (SRM) analysis and from combined full scan and SRM analysis (SRM/EMS) were compared. The quantitative data in both scan modes are acceptable in terms of sensitivity, accuracy and precision. One can conclude from this work that the hybrid linear ion trap/triple quadrupole mass analyzer can provide in a single analysis both useful qualitative data, and accurate and precise quantitative data from the samples routinely prepared and analyzed for target drug concentrations.
Department of Drug Metabolism, Merck and Co., Inc., West Point, PA 19486, USA. richard_king@merck.com
Richard C
RC
King
Richard
R
Gundersdorf
Carmen L
CL
Fernández-Metzler
D000818:Animals; D004355:Drug Stability; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010879:Piperazines / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013997:Time Factors
D010879:Piperazines; C015568:urapidil