Methods for the identification of differentially expressed genes in human post-mortem brain.
Microarrays can be used to monitor the expression of thousands of genes simultaneously. This technique requires high-quality RNA which can be extracted from a variety of tissues and cells including post-mortem human brain. Given the vast amount of information obtained from microarray studies, it is critical to establish valid analysis techniques to identify differentially expressed genes. This technical report describes the basic methodology and analyses used to identify such genes in human post-mortem brain tissue.
Mayfield
R Dayne
RD
Department of Psychology, Waggoner Center for Alcohol and Addiction Research, Molecular Biology Building 1.124, University of Texas at Austin, Austin, TX 78712, USA. dayne.mayfield@mail.utexas.edu
Liu
Jianwen
J
Randall
Patrick K
PK
Lewohl
Joanne M
JM
Dodd
Peter R
PR
Harris
R Adron
RA
D001923:Brain Chemistry / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D012494:Sampling Studies