Antioxidant activity of extracts from Alpinia katsumadai seed.
Alpinia katsumadai (Zingiberaceae) has been widely used in traditional Chinese medicine to treat a variety of conditions such as emesis and gastric disorders. However, very little is known about the cellular actions by which this plant mediates its therapeutic effects. Various aspects of antioxidant activity were evaluated in a total extract derived from Alpinia katsumadai seed in this study. Relatively high levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were detected in the total extract (IC(50) 1.6 microgram/mL). Other known compounds such as (-)-epigallocatechine-3-gallate (EGCG) and resveratrol showed IC(50) values of <0.8 and 4.8 microgram/mL, respectively. The total extract also enhanced the viability of Chinese hamster lung fibroblast (V79-4) cells and inhibited H(2)O(2)-induced apoptosis. The total extract of Alpinia katsumadai also dose-dependently enhanced the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in V79-4 cells, and these effects were comparable to other antioxidant compounds such as EGCG and resveratrol. Taken together, our findings show that Alpinia katsumadai contains significant antioxidant activity.
Department of Oral Biochemistry, College of Dentistry, Kyung Hee University Seoul 130-701, Korea.
Si Eun
SE
Lee
Hyun-Tak
HT
Shin
Hyun Jin
HJ
Hwang
Jeong Hee
JH
Kim
D032429:Alpinia; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D006861:Hydrogen Peroxide; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl