The nephrotoxicity of Aristolochia manshuriensis in rats is attributable to its aristolochic acids.
BACKGROUND
Although Aristolochia manshuriensis (AM), which was incriminated in the Japanese variety of Chinese herbs nephropathy, has been recently shown to be nephrotoxic in rats, less is known about whether this toxicity is attributable to its aristolochic acids (AA). We compared the renal effect of AM with that of Akebia quinata (AQ), which has similar components to AM but is free of AA; we also compared this effect of AM with that of pure AA, and studied its possible mechanism.


METHODS
In study 1, rats were divided into four groups. Each group was orally given either 0.4 g of AM, 4 g of AM, or 4 g of AQ per day, or vehicle, for 5 days. In study 2, rats were given 4 g of AM (which contained 4 mg of AA), or 4 mg of pure AA per day, or vehicle, for 5 days. Renal function, and renal histology were evaluated on day 5 in study 1 and on days 1, 3, 5, and 14 in study 2. In study 2, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) staining were also performed. In study 3, rats were treated in the same way as in study 2, but were all killed on day 5. Concentrations of AA (I + II) were measured in serum and urine in study 2, and in the kidney, lung, heart, liver, and spleen in study 3.


RESULTS
In study 1, only the rats that received 4 g/day of AM developed tubular necrosis, azotemia, proteinuria, and glucosuria. In study 2, both AM- and AA-treated rats showed progressive tubular damage, decreased renal function, and increased urinary protein excretion. The degree of these alterations was comparable in the AM and AA groups. Moreover, the concentrations of AA (I + II), in serum, urine, and kidney were also comparable in the AM- and AA-treated rats. High levels of AA were detected in the lung and kidney. Apoptotic tubular cells increased on day 5 and had decreased by day 14 after the withdrawal of AM or AA. Meanwhile, proliferating tubular cells progressively increased from day 3 to day 14.


CONCLUSIONS
Our study indicates that the renal toxicity of AM can be attributed to its AA component. Tubular cell apoptosis might be one of the mechanisms involved in this renal injury.
Division of Clinical Immunology, Department of Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Man-Chang
MC
Liu
Shoichi
S
Maruyama
Masashi
M
Mizuno
Yoshiki
Y
Morita
Shigeru
S
Hanaki
Yukio
Y
Yuzawa
Seiichi
S
Matsuo
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D030022:Aristolochia / Q000633:toxicity*; D034341:Aristolochic Acids / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000401:mortality / Q000473:pathology; D018514:Plant Structures / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D015996:Survival Rate
D034341:Aristolochic Acids; C000228:aristolochic acid I; D003404:Creatinine