RNA interference with small hairpin RNAs transcribed from a human U6 promoter-driven DNA vector.
RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of organisms. Recently, a small-interference RNA (siRNA) technology has been reported to produce post-transcriptional gene silencing in mammalian cells. In the present study, we constructed a human U6 promoter-driven mammalian expression vector to produce hairpin double-stranded RNA and transfected this into a human cell line. Using this siRNA system, we were able to knock down the gene expression of an enhanced green fluorescence protein. This result indicates that the plasmid vector-based siRNA system is a promising method to downregulate gene expression in human cells.
Kwak
Young-Don
YD
Department of Psychiatry, University of Illinois at Chicago, IL 60612, USA.
Koike
Hiroko
H
Sugaya
Kiminobu
K
D002460:Cell Line; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D034904:Lamin Type A / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D034622:RNA Interference / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects
D034904:Lamin Type A; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D012313:RNA; D004247:DNA