Binding of perfluorinated fatty acids to serum proteins.
Perfluorooctane sulfonic acid (PFOS) accumulates in the liver and blood of exposed organisms. The potential for these surfactant molecules to interfere with hormone/protein interactions in blood is of concern given the importance of these interactions. The PFOS binding to serum proteins was investigated by assessing its ability to displace a variety of steroid hormones from specific binding proteins in the serum of birds and fishes. Perfluorooctane sulfonic acid had only a weak ability to displace estrogen or testosterone from carp serum steroid binding proteins. Displacement of cortisone in avian sera occurred at relatively low PFOS concentrations. Corticosterone displacement potency increased with chain length, and sulfonic acids were more potent than carboxylic acids. The PFOS concentrations estimated to cause these effects were 320 microM or greater, equivalent to serum concentrations greater than 160 mg/L. Using mass spectrometry and direct in vitro binding assays, PFOS was demonstrated to bind strongly to bovine serum albumin (BSA) in a 1:1 stoichiometric ratio. It appears that PFOS in serum is in general bound to albumins. Concentrations of PFOS required to saturate albumin would be in excess of 50 to 100 mg/L. Based on current environmental concentrations, it is unlikely that PFOS would cause displacement of hormones from serum proteins in wildlife.
Jones
Paul D
PD
Department of Zoology, National Food Safety and Toxicology Center, Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan 48824, USA. jonespa7@msu.edu
Hu
Wenyue
W
De Coen
Wim
W
Newsted
John L
JL
Giesy
John P
JP
D017738:Alkanesulfonic Acids / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D000835:Animals, Wild; D001717:Birds; D001798:Blood Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004703:Endocrine System / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry*; D005399:Fishes; D005466:Fluorocarbons / Q000737:chemistry* / Q000633:toxicity; D006728:Hormones / Q000494:pharmacology
D017738:Alkanesulfonic Acids; D001798:Blood Proteins; D005227:Fatty Acids; D005466:Fluorocarbons; D006728:Hormones; C076994:perfluorooctane sulfonic acid