Identification of a very long chain polyunsaturated fatty acid Delta4-desaturase from the microalga Pavlova lutheri.
Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Delta4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.
CNAP, Department of Biology, University of York, Heslington, YO10 5YW, York, UK.
Thierry
T
Tonon
David
D
Harvey
Tony R
TR
Larson
Ian A
IA
Graham
D000595:Amino Acid Sequence; D002849:Chromatography, Gas; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D056890:Eukaryota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D044943:Fatty Acid Desaturases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D044943:Fatty Acid Desaturases; C434953:delta-4 fatty acid desaturase