Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen.
A comprehensive analysis of culture supernatant (CSN) proteins of Mycobacterium tuberculosis H37Rv was accomplished by combination of two-dimensional electrophoresis (2-DE), mass spectrometry, and N-terminal sequencing by Edman degradation. Analytical 2-DE gels resolved approximately 1250 protein spots from CSN of M. tuberculosis H37Rv, 381 of which were identified by mass spectrometry and/or Edman degradation. This study revealed 137 different proteins, 42 of which had previously been described as secreted. Comparative proteome analysis of CSN from virulent M. tuberculosis H37Rv and attenuated Mycobacterium bovis BCG Copenhagen identified 39 M. tuberculosis-specific spots containing 27 different proteins, representing candidate antigens for novel vaccines and diagnostics in tuberculosis. These included five proteins encoded by open reading frames absent from M. bovis BCG, e.g., early secretory antigen target (Esat6), as well as 22 novel differential proteins, such as acetyl-CoA C-acetyltransferase (Rv0243) and two putative Esat6-like proteins (Rv1198, Rv1793).
Mattow
Jens
J
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany. mattow@mpiib-berlin.mpg.de
Schaible
Ulrich E
UE
Schmidt
Frank
F
Hagens
Kristine
K
Siejak
Frank
F
Brestrich
Gordon
G
Haeselbarth
Gisela
G
Müller
Eva-Christina
EC
Jungblut
Peter R
PR
Kaufmann
Stefan H E
SH
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D009163:Mycobacterium bovis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned