Antiproliferative effects of several compounds isolated from Amburana cearensis A. C. Smith.
Amburana cearensis a common tree found in Northeastern Brazil is widely used in folk medicine. The present work evaluated the cytotoxicity of kaempferol, isokaempferide, amburoside A and protocatechuic acid isolated from the ethanol extract of the trunk bark of A. cearensis. The compounds were tested for their cytotoxicity on the sea urchin egg development, hemolysis assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay using tumor cell lines. Isokaempferide and kaempferol, but not amburoside A and protocatechuic acid, inhibited the sea urchin egg development as well as tumor cell lines, but in this assay isokaempferide was more potent than kaempferol. Protocatechuic acid was the only compound able to induce hemolysis of mouse erythrocytes, suggesting that the cytotoxicity of kaempferol and isokaempeferide was not related to membrane damage.
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, P.O. Box-3157, 60430-270 Fortaleza, Ceará, Brazil. lvcosta@secrel.com.br
Letícia V
LV
Costa-Lotufo
Paula C
PC
Jimenez
Diego V
DV
Wilke
Luzia Kalyne A M
LK
Leal
Geanne M A
GM
Cunha
Edilberto R
ER
Silveira
Kirley M
KM
Canuto
Glauce S B
GS
Viana
Maria Elisabete A
ME
Moraes
Manoel Odorico
MO
de Moraes
Cláudia
C
Pessoa
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044949:Kaempferols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010058:Oviposition / Q000187:drug effects; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012617:Sea Urchins; D012639:Seeds / Q000737:chemistry; D014197:Trees / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D062385:Hydroxybenzoates; D044949:Kaempferols; C009091:protocatechuic acid