Assay of salsolinol in peripheral blood mononuclear cells of alcoholics and healthy subjects by gas chromatography-mass spectrometry.
Endogenous 6,7-dihydroxy-1-methyl-1,2,3,4-tetrahydroisoquinoline (salsolinol) could be a potential marker involved in the aetiology of alcoholism. Whereas the amount of salsolinol in plasma and urine depends on several dietary conditions, the salsolinol from peripheral mononuclear cells should be formed endogenously. Salsolinol was quantified in lymphocytes of 10 controls and 11 alcoholics (after 1 and 13 weeks of abstinence) using solid phase extraction and gas chromatography/mass spectrometry. Alcoholics showed significantly lower salsolinol concentration than the controls. After 13 weeks of abstinence a further significant decrease of SAL levels could be seen in the lymphocytes of alcoholics. The findings of this study support the theory that salsolinol might be a trait marker in alcoholism.
Haber
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Institute of Pharmacy, Humboldt-University, Goethestrasse 54, D-13086 Berlin, Germany.
Jahn
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Ehrenreich
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Melzig
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MF
D000328:Adult; D000437:Alcoholism / Q000097:blood* / Q000175:diagnosis / Q000523:psychology; D016739:Behavior, Addictive / Q000523:psychology; D015415:Biomarkers; D039721:Diagnostic and Statistical Manual of Mental Disorders; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation; D006304:Health Status; D006801:Humans; D007546:Isoquinolines / Q000097:blood*; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D013695:Temperance
D015415:Biomarkers; D007546:Isoquinolines; C036617:salsolinol