v-src oncogene-specific carboxy-terminal peptide is immunoprotective against Rous sarcoma growth in chickens with MHC class I allele B-F12.
B(12) haplotype of the inbred chicken line CB (B12/B12) contains, like the bulk of chicken MHC(B) haplotypes, only a single dominantly expressed class I molecule (B-F). The peptide binding motifs for this major B-F12 molecule in chickens of Rous sarcoma regressor line CB (B12/B12) have been determined. Using stringent and relaxed motifs, several peptides were found in the v-src molecule of the PR-RSV-C, but most of these peptides are identical with that of endogenous c-src. Only the v-src C-tail peptide(517-524) (LPACVLEV) contains critical anchor amino acids (valine at positions 5 and 8) and shows a sequence different from the corresponding c-src peptide. This v-src C-tail peptide up-regulates expression of the B-F12 class I molecule on PBL, as assessed by FACS analysis, and stimulates T cell proliferation in a [3H]thymidine uptake assay. A protective effect of the immune response to LPACVLEV against RSV challenge was demonstrated in CB (B12/B12) chickens immunised with peptides encapsulated in liposomes.
Medical School, Institute for Pathophysiology, University of Innsbruck, Fritz Pregl Strasse 3, A-6020 Innsbruck, Austria.
Andrea
A
Hofmann
Jirí
J
Plachy
Lawrence
L
Hunt
Jim
J
Kaufman
Karel
K
Hala
D000483:Alleles; D000818:Animals; D001358:Avian Sarcoma Viruses; D019496:Cancer Vaccines / Q000276:immunology*; D002455:Cell Division; D002645:Chickens; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008081:Liposomes; D008863:Microspheres; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D001357:Sarcoma, Avian / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015854:Up-Regulation; D014611:Vaccination / Q000662:veterinary
D019496:Cancer Vaccines; D015395:Histocompatibility Antigens Class I; D008081:Liposomes; C098080:MICB antigen; D010446:Peptide Fragments; D015688:Oncogene Protein pp60(v-src)