Green fluorescent protein-labeled mapping of neural stem cells migrating towards damaged areas in the adult central nervous system.
Neural stem cells, which are clonogenic cells with multilineage differentiation properties from regions of the fetal brain, cortex and hippocampus, are currently considered as powerful candidates for cell replacement therapy in neurodegenerative disorders, such as Parkinson's disease. A key issue is whether stem cells can survive, migrate and differentiate following transplantation into the adult CNS. Here, enhanced green fluorescent protein plasmid electroporation-transfected neural stem cells from the fetal cortex were grafted into the striatum of a rat model of Parkinson's disease. We found most of the grafted cells could survive in the adult parkinsonian rat brain and migrated towards damaged areas, while they moved randomly in the normal brain. Several grafted cells differentiated into neurons.
Zhou
Chang
C
Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane, 100084, Beijing, China.
Wen
Zhe-Xing
ZX
Wang
Zhi-Ping
ZP
Guo
Xing
X
Shi
Dong-Mei
DM
Zuo
Huan-Cong
HC
Xie
Zuo-Ping
ZP
D000818:Animals; D001931:Brain Mapping; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000637:transplantation; D003342:Corpus Striatum / Q000166:cytology / Q000196:embryology / Q000601:surgery; D004195:Disease Models, Animal; D018274:Electroporation; D006085:Graft Survival / Q000502:physiology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D010300:Parkinson Disease / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins