[Effects of simulated weightlessness and mechanical loading on bone interstitial fluid flow in rats].
OBJECTIVE
To investigate the effects of simulated weightlessness and mechanical loading on bone interstitial fluid flow.


METHODS
Thirty-six male Sprague-Dawley [correction of Spargue-Danley] rats were divided into 3 groups: the control group, the tail-suspension group, and the tail-suspension plus mechanical loading group, with four rats in each group. All the rats were injected via a lateral tail vein with horseradish peroxidase on the 21st day of the experiment. Tibial tissue specimens were explanted, fixed, decalcified and cut into 30 micrometers frozen sections 3 h after the intravenous injection.


RESULTS
There were less peroxidase reaction product in the bony matrix and bone lacunae in tail-suspended rats than the control and tail-suspended plus mechanical loading rats.


CONCLUSIONS
Tail-suspension decreased fluid movement through the bone, while mechanical loading increased it.
Institute of Space Medico-Engineering, Beijing, China.
Yong-jie
YJ
Ma
Yan-hong
YH
Yuan
Li-qin
LQ
Xie
Ying-hui
YH
Li
Yu-min
YM
Wan
Zhi-zheng
ZZ
Shi
D000818:Animals; D001857:Bone Matrix / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D045604:Extracellular Fluid / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D019417:Hindlimb Suspension; D006735:Horseradish Peroxidase / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D013314:Stress, Mechanical; D013977:Tibia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016474:Weight-Bearing; D018474:Weightlessness Simulation
D006735:Horseradish Peroxidase