Dietary G-rutin suppresses glycation in tissue proteins of streptozotocin-induced diabetic rats.
The present study focused on examining the efficacy of feeding a rutin-glucose derivative (G-rutin) to inhibit glycation reactions that can occur in muscle, kidney and plasma proteins of diabetic rats. Both thiobarbituric acid-reactive substance levels and protein carbonyl contents in muscle and kidney were significantly (p < 0.05) reduced in streptozotocin-induced diabetic rats fed G-rutin supplemented diet, compared to diabetic rats fed control diet. The N(epsilon)-fructoselysine content in muscle and kidney, a biomarker of early glycation reaction, was markedly (p < 0.05) increased by diabetes, but significantly (p < 0.05) reduced in diabetic rats fed G-rutin. Advanced glycation end-products (AGEs) in serum and kidney protein were measured by immunoblot using anti-AGE antibody, and were also reduced in diabetic rats fed dietary G-rutin. Feeding G-rutin also slightly inhibited aldose reductase activity in these animals. These results demonstrate for the first time that dietary G-rutin consumption can provide potential health benefits that are related to the inhibition of tissue glycation reactions common to diabetes.
Food and Health Science, Faculty of Agriculture, Iwate University, Morioka, Iwate, Tokyo, Japan.
Takashi
T
Nagasawa
Nobuaki
N
Tabata
Yoshiaki
Y
Ito
Youichi
Y
Aiba
Naoyuki
N
Nishizawa
David D
DD
Kitts
D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004032:Diet; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000008:administration & dosage / Q000494:pharmacology*; D013311:Streptozocin
D017127:Glycation End Products, Advanced; C033186:fructosyl-lysine; D012431:Rutin; D013311:Streptozocin; D005947:Glucose; D008239:Lysine