A bipartite late-budding domain in human immunodeficiency virus type 1.
Human immunodeficiency virus type 1 (HIV-1) encodes a PTAP motif within the p6 domain of Gag that recruits Tsg101 and associated factors to facilitate virion budding. In this study, we use trans-complementation assays to demonstrate that the PTAP motif acts synergistically with additional p6 sequences to mediate the formation of infectious extracellular HIV-1 virions. These studies suggest that Tsg101 recruitment is necessary but not sufficient to account for late-budding activity exhibited by HIV-1 p6.
Aaron Diamond AIDS Research Center, New York, New York, USA.
Juan
J
Martin-Serrano
Paul D
PD
Bieniasz
D020816:Amino Acid Motifs; D015683:Gene Products, gag / Q000737:chemistry / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D025801:Ubiquitin / Q000378:metabolism; D014771:Virion / Q000502:physiology
D015683:Gene Products, gag; D025801:Ubiquitin