Antibody subclass profile against Leishmania braziliensis and Leishmania amazonensis in the diagnosis and follow-up of mucosal leishmaniasis.
Sensitivities and specificities of IgG subclasses-ELISA and IgG-indirect fluorescent antibody test (IFAT) against Leishmania braziliensis (Lb) and L. amazonensis (La) antigens were determined in 17 patients with mucosal (ML) and 19 with muco-cutaneous (MCL) leishmaniasis. Using IFAT-IgG both antigens gave high sensitivities and were statistically similar, being 89.5% with La and 100% with Lb. Using ELISA, the highest sensitivity was achieved with total IgG for ML (94.7% with both antigens) and MCL (100% with both antigens). Cross-reactivity, observed with Chagas disease and malaria sera reduced the specificity of the IgG-based assays, being 50 to 70% with IFAT and 40 to 70% with ELISA. An increase in specificity was obtained with IgG1-ELISA (90% with Lb and 100% with La). Serum levels of anti-Lb-IgG and IgG3 dropped 90 days after treatment. IgG subclasses antibody detection constitute an valuable alternative to increase the efficiency of sorological diagnostics of ML/MCL.
Laboratory of Clinical Research, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, MG, Belo Horizonte, Brazil.
Mariana
M
Junqueira Pedras
Marcela
M
Orsini
Marcelo
M
Castro
Valéria M A
VM
Passos
Ana
A
Rabello
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis / Q000145:classification*; D001938:Brazil; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007892:Leishmania braziliensis / Q000276:immunology* / Q000302:isolation & purification; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000276:immunology; D007897:Leishmaniasis, Mucocutaneous / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011336:Probability; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G