[Towards the positional cloning of a spikelet identity gene frizzle panicle (FZP) in rice (Oryza sativa L.)].
FZP is a key gene for spikelet differentiation in rice. Mutation of the gene blocks the differentiation of spikelets and makes rachis branches develop unlimitedly. A mutant of the gene named frizzle panicle (fzp) was previously found from the high-generation progeny of a cross between two Oryza sativa ssp. indica rice varieties, V20B and Hua1B. With the mutant, FZP had been mapped to a chromosomal region of about 26.4 cM in width between two SSR (Simple Sequence Repeat) markers, RM172 and RM18, on chromosome 7. In this study, high-resolution mapping of the gene was carried out for the positional cloning of the gene. Two flanking SSR markers, NRM6 and NRM8, were identified, which are 0.2 cM and 1.0 cM apart from the target gene, respectively, bracketing the target gene within an interval of 1.2 cM or 144 kb. An APETALA2 (AP2)-domain like gene was found at the expected position of FZP. As AP2 is known to play an important role in the floral development, we took it as the most possible candidate of FZP. PCR analysis showed that the mutant allele of the AP2-domain like gene contains an insert of about 4 kb in length, suggesting that the gene is very likely FZP.
College of Crop Science, Fujian Agriculture and Forestry University, Key Laboratory of Genetic Engineering, Fujian Academy of Agricultural Sciences, Fuzhou, China.
Hua-Qing
HQ
Liu
Wei-Ren
WR
Wu
Yuan-Lin
YL
Duan
Hua-Zhong
HZ
Guan
Juan
J
Chen
Wei-Ming
WM
Li
Yong-Biao
YB
Xue
D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018895:Microsatellite Repeats; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*
D010940:Plant Proteins