Pluronic L81 affects the lipid particle sizes and apolipoprotein B conformation.
The chylomicron assembly has been proposed to involve the core expansion of apolipoprotein B (apoB)-containing primordial lipoproteins by fusing with triglyceride-rich lipid droplets. We examined the effects of an inhibitor of chylomicron secretion, Pluronic L81, on triolein-phosphatidylcholine emulsions and low density lipoproteins (LDL) which were used for the models of lipid droplets and primordial lipoproteins, respectively. We showed by dynamic light scattering that the sizes of lipid emulsions and LDL were increased in the presence of Pluronic L81. The binding of apoB-100 to lipid emulsions was enhanced by Pluronic L81. CD and fluorescence lifetime measurements revealed that Pluronic L81 altered the secondary structure of apoB-100 with an increased local hydration. The proper hydrophilic-to-hydrophobic balance of Pluronic L81 is important for these actions. It is proposed that Pluronic L81 inhibits the secretion of chylomicrons by leading the excess core expansion of the primordial lipoproteins and the conformational modification of apoB.
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Shin-ya
SY
Morita
Misa
M
Kawabe
Minoru
M
Nakano
Tetsurou
T
Handa
D001055:Apolipoproteins B / Q000737:chemistry* / Q000187:drug effects; D002914:Chylomicrons / Q000037:antagonists & inhibitors; D004655:Emulsions; D006801:Humans; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D010316:Particle Size; D020442:Poloxamer / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*
D001055:Apolipoproteins B; D002914:Chylomicrons; D004655:Emulsions; D008077:Lipoproteins, LDL; D013501:Surface-Active Agents; D020442:Poloxamer