Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products.
Specialty sorghums, their brans, and baked and extruded products were analyzed for antioxidant activity using three methods: oxygen radical absorbance capacity (ORAC), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). All sorghum samples were also analyzed for phenolic contents. Both ABTS and DPPH correlated highly with ORAC (R(2) = 0.99 and 0.97, respectively, n = 18). Phenol contents of the sorghums correlated highly with their antioxidant activity measured by the three methods (R(2) >or= 0.96). The ABTS and DPPH methods, which are more cost effective and simpler, were demonstrated to have similar predictive power as ORAC on sorghum antioxidant activity. There is a need to standardize these methods to allow for data comparisons across laboratories.
Awika
Joseph M
JM
Cereal Quality Lab, Soil & Crop Sciences Department, Texas A&M University, College Station, Texas 77843, USA. jawika@tamu.edu
Rooney
Lloyd W
LW
Wu
Xianli
X
Prior
Ronald L
RL
Cisneros-Zevallos
Luis
L
D000975:Antioxidants / Q000032:analysis*; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D012680:Sensitivity and Specificity; D045868:Sorghum / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D017382:Reactive Oxygen Species; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl