Antimicrobial peptides from scorpion venom induce Ca(2+) signaling in HL-60 cells.
Parabutoporin (PP) and opistoporin 1 (OP1) are amphipathic alpha-helical antimicrobial peptides that were recently isolated from scorpion venom. In assays in which single granulocyte-like HL-60 cells as well as cells in suspension were used, both peptides were able to induce a reversible Ca(2+) release from intracellular stores and to increase Ca(2+) influx. Both effects could be clearly differentiated for OP1, inducing Ca(2+) release at lower concentrations. The Ca(2+) release was pertussis toxin-sensitive indicating the involvement of G-proteins. Ca(2+) release depended on the stage of differentiation of the cells with undifferentiated cells being the most sensitive. Desensitization occurred with OP1. No cross-desensitization occurred between OP1 and the bacterial chemoattractant fMLP indicating the involvement of different types of receptors. Ca(2+) release by OP1 was found not to be mediated via interaction with the formyl peptide receptor-like 1. Although some of the results might favor a receptor-like interaction, the receptor involved could not be identified.
Interdisciplinary Research Center, Catholic University Leuven Campus Kortrijk, Belgium. Leentje.Moerman@kulak.ac.be
Leentje
L
Moerman
Fons
F
Verdonck
Jean
J
Willems
Jan
J
Tytgat
Suzanne
S
Bosteels
D023181:Antimicrobial Cationic Peptides / Q000145:classification* / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D012604:Scorpion Venoms / Q000737:chemistry / Q000494:pharmacology*
D023181:Antimicrobial Cationic Peptides; D012604:Scorpion Venoms; D009240:N-Formylmethionine Leucyl-Phenylalanine