Helicobacter pylori cag pathogenicity island is associated with reduced expression of interleukin-4 (IL-4) mRNA and modulation of the IL-4delta2 mRNA isoform in human gastric mucosa.
Interleukin-4 (IL-4) and IL-4delta2 mRNA gastric expression was evaluated in healthy subjects and patients who did not have ulcers but were infected with Helicobacter pylori with or without the cag pathogenicity island (cag PAI). IL-4 mRNA was physiologically expressed by gastric epithelium and negatively influenced by H. pylori. Also, nonepithelial cells in the lamina propria of H. pylori-infected patients expressed IL-4 mRNA, whereas IL-4delta2 mRNA was found only in cag PAI-negative patients. Thus, gastric IL-4 takes part in the local immune response to H. pylori.
Orsini
Barbara
B
Department of Clinical Pathophysiology, University of Florence, Florence, Italy. b.orsini@dfc.unifi.it
Ottanelli
Barbara
B
Amedei
Amedeo
A
Surrenti
Elisabetta
E
Capanni
Marco
M
Del Prete
Gianfranco
G
Amorosi
Andrea
A
Milani
Stefano
S
D'Elios
Mario Milco
MM
Surrenti
Calogero
C
D000328:Adult; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence
D020033:Protein Isoforms; D012333:RNA, Messenger; D015847:Interleukin-4