Adhesion and proliferation of human endothelial cells on photochemically modified polytetrafluoroethylene.
We studied the adhesion and proliferation of human endothelial cells on photochemically modified polytetrafluoroethylene samples. The polymer surfaces were modified by exposure to the ultraviolet light of a Xe(2)(*)-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere. Treatment times were between 10 and 20 min. The endothelial cell density was determined 1, 3 and 8 days after seeding by image analysis. Surface modification of the samples resulted in a significant increase in the number of adhering cells and in the formation of a confluent cell layer after 3-8 days. The results were comparable than those obtained on polystyrene Petri dishes, which are used as standard substrates in cell cultivation. Thus modified PTFE appears to be a promising material for the fabrication of artificial vascular prostheses coated with endothelial cells.
Department of Applied Physics, Johannes Kepler University, Linz A-4040, Austria.
T
T
Gumpenberger
J
J
Heitz
D
D
Bäuerle
H
H
Kahr
I
I
Graz
C
C
Romanin
V
V
Svorcik
F
F
Leisch
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010777:Photochemistry / Q000379:methods; D011138:Polytetrafluoroethylene / Q000737:chemistry* / Q000528:radiation effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene