A high mobility group protein from the Dipteran insects Ceratitis capitata and Bactrocera oleae.
Nuclei from Bactrocera oleae and Ceratitis capitata larvae contain a major protein that shares most of the characteristics of vertebrate high mobility group (HMG) proteins. Proteins are extracted from nuclei with 0.35 M NaCl, are soluble in 5% perchloric acid, are relatively small (molecular weight in the range of 10-16 kDa), and have both a high basic and a high acidic amino acid content. The amino acid constitution of these proteins is similar to that of the HMGB protein family of vertebrates. The proteins cross-react with antibodies raised against the HMGD chromosomal protein of Drosophila melanogaster. The possible relatedness of these proteins to high mobility group proteins is discussed.
Department of Biology, Division of Genetics and Biotechnology, University of Athens, Panepistimiopolis 15701, Athens, Greece. valepor@biol.uoa.gr
Vassiliki
V
Aleporou-Marinou
Christina
C
Deli
Yiannis
Y
Ninios
Barbara
B
Agelopoulou
Haroula
H
Marinou
Theocharis
T
Patargias
D000818:Animals; D015153:Blotting, Western; D033622:Ceratitis capitata / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D033621:Tephritidae / Q000235:genetics / Q000378:metabolism
D006609:High Mobility Group Proteins; D006657:Histones; D009687:Nuclear Proteins