Early divergence of the FtsZ1 and FtsZ2 plastid division gene families in photosynthetic eukaryotes.
Homologues of the bacterial cell division protein FtsZ are found in higher plants where they function as key components of the chloroplast division complex. In contrast to most bacteria that encode a single FtsZ protein, plants encode multiple proteins that group into two families, FtsZ1 and FtsZ2. Using new sequence data from a broad range photosynthetic organisms, we performed a series of analyses to better understand the evolutionary history of the plant FtsZ families. Multiple phylogenetic analyses strongly support the grouping of the plant FtsZ genes and proteins into distinct FtsZ1 and FtsZ2 clades. Protein features representing potentially significant functional differences between FtsZ1 and FtsZ2 are identified. Genomic structure comparisons show that exon length and intron position are conserved within each clade, but differ between the clades except at one position. Our data indicate that the divergence of the FtsZ1 and FtsZ2 families occurred long before the evolution of land plants, preceding the emergence of the green algae. The results are consistent with proposals that the two FtsZ families evolved distinct functions during evolution of the chloroplast division apparatus, and indicate that genetic and functional differentiation occurred much earlier than previously hypothesized.
Stokes
Kevin D
KD
Department of Plant Biology, Michigan State University, 166 Plant Biology Building, East Lansing, MI 48824-1312, USA.
Osteryoung
Katherine W
KW
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000502:physiology; D019143:Evolution, Molecular; D014644:Genetic Variation; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C095113:FTSZ protein, Arabidopsis; C071525:FtsZ protein, Bacteria; D010940:Plant Proteins; D020033:Protein Isoforms