Development of a system for the study of protein-protein interactions in planta: characterization of a TATA-box binding protein complex in Oryza sativa.
We describe a simple, rapid method for protein complex purification in planta. Using a biotin peptide as an affinity tag with TATA-box binding protein (TBP), 86 unique proteins present in the purified complex were identified by tandem mass spectrometry. We identified proteins known to be associated with TBP, and many other proteins involved in pre-mRNA processing and chromatin remodeling. The identification of these novel protein-protein associations will upon further investigations provide new insights into the mechanisms of mRNA transcription and pre-mRNA processing.
Department of Functional Genomics, Torrey Mesa Research Institute, Syngenta Research and Technology, San Diego, California 92121, USA.
Jingping
J
Zhong
Paul A
PA
Haynes
Shiping
S
Zhang
Xinping
X
Yang
Nancy L
NL
Andon
Donna
D
Eckert
John R
JR
Yates
Xun
X
Wang
Paul
P
Budworth
D000595:Amino Acid Sequence; D001710:Biotin / Q000737:chemistry; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D016208:Databases, Factual; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016622:Silver Staining; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics
D011506:Proteins; D011993:Recombinant Fusion Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D001710:Biotin