DANCER: a program for digital anatomical reconstruction of gene expression data.
A digital anatomy construction (DANCER) program was developed for gene expression data. DANCER can be used to reconstruct anatomical images from in situ hybridization images, microarray or other gene expression data. The program fills regions of a drawn figure with the corresponding values from a gene expression data set. The output of the program presents the expression levels of a particular gene in a particular region relative to other regions. The program was tested with values from experimental in situ hybridization autoradiographs and from a microarray experiment. Reconstruction of in situ hybridization data from adult rat brain made by DANCER corresponded well with the original autoradiograph. Reconstruction of microarray data from adult mouse brains provided images that reflect actual expression levels. This program should help to provide visualization and interpretation of data derived from gene expression experiments. DANCER may be freely downloaded.
Kankainen
Matti
M
Laboratory of Functional Genomics and Bioinformatics, Department of Neurobiology, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, PO Box 1627, FIN-70211, Kuopio, Finland.
Wong
Garry
G
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017403:In Situ Hybridization; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D012680:Sensitivity and Specificity; D012984:Software; D014584:User-Computer Interface