A transgenic analysis of mouse lactate dehydrogenase C promoter activity in the testis.
Transcription of the mouse testis-specific lactate dehydrogenase c (mldhc) gene is limited to cells of the germinal epithelium. Cloning and analysis of the mldhc promoter revealed that a 100-bp core promoter was able to regulate testis-specific transcription in vitro and in transgenic mice. Surprisingly, expression of the reporter in transgenic testes was limited to pachytene spermatocytes, whereas native LDH-C(4) was detected in pachytene and all later germ cells. To locate additional regulatory sequence that could recapitulate the native LDH-C(4) distribution pattern, we investigated the contribution of 5' and 3' flanking sequences to the regulation of LDH-C(4) expression. We found that transcription factor YY1 binds to the mldhc promoter, that the mldhc 3' untranslated sequence does not permit a postmeiotic expression of a beta-galactosidase reporter in transgenic mice, and that native mldhc mRNA is predominately meiotic, with only a low level of postmeiotic distribution. Our results suggest that the high level of LDH-C(4) in postmeiotic cells results from mRNA and protein stability.
Department of Biochemistry, Northwestern University, Chicago, Illinois, USA.
Tim L
TL
Kroft
Siming
S
Li
Lynn
L
Doglio
Erwin
E
Goldberg
D024509:3' Flanking Region / Q000502:physiology; D024506:5' Flanking Region / Q000502:physiology; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D050981:Erythroid-Specific DNA-Binding Factors; D015870:Gene Expression / Q000502:physiology; D017930:Genes, Reporter; D007527:Isoenzymes / Q000235:genetics*; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008297:Male; D008540:Meiosis / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology; D050986:YY1 Transcription Factor; D001616:beta-Galactosidase / Q000235:genetics
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D007527:Isoenzymes; D011506:Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4; D001616:beta-Galactosidase