Solution structure of epiregulin and the effect of its C-terminal domain for receptor binding affinity.
Epiregulin (EPR), a novel member of epidermal growth factor (EGF) family, is a ligand for ErbB-1 and ErbB-4 receptors. The binding affinity of EPR for the receptors is lower than those of other EGF-family ligands. The solution structure of EPR was determined using two-dimensional nuclear magnetic resonance spectroscopy. The secondary structure in the C-terminal domain of EPR is different from other EGF-family ligands because of the lack of hydrogen bonds. The structural difference in the C-terminal domain may provide an explanation for the reduced binding affinity of EPR to the ErbB receptors.
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-0194 Toyama, Japan.
Katsuharu
K
Sato
Takashi
T
Nakamura
Mineyuki
M
Mizuguchi
Kazunori
K
Miura
Masahito
M
Tada
Tomoyasu
T
Aizawa
Tomoharu
T
Gomi
Kaoru
K
Miyamoto
Keiichi
K
Kawano
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D066260:Epiregulin; D066246:ErbB Receptors / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012996:Solutions / Q000737:chemistry
D066260:Epiregulin; D011993:Recombinant Fusion Proteins; D012996:Solutions; D004815:Epidermal Growth Factor; D066246:ErbB Receptors