Enhanced cell attachment and osteoblastic activity by P-15 peptide-coated matrix in hydrogels.
The cells in bone grow on a composite matrix made up of mineral and organic (mainly type-I collagen) components. In this study, anorganic bone mineral (ABM) particles were coated with a cell-binding domain of type-I collagen (P-15 peptide) to mimic the bone matrix components and suspended in injectable hyaluronate (Hy) hydrogels. The ABM/P-15/Hy was compared to ABM/Hy-the same matrix without P-15 peptide. Osteoblast-like HOS cells migrated through the hydrogels around ABM/P-15 or ABM particles; however, more cells adhered to ABM/P-15/Hy particles, and the cells formed better surface coverage and had more stress fibers on ABM/P-15/Hy. HOS cells cultured on ABM/P-15/Hy had increased osteogenic gene expression for alkaline phosphatase and bone morphogenetic proteins, and deposited more mineralized matrix. Studies with two different hydrogels (carboxymethylcellulose and sodium alginate) showed similar enhanced cell attachment and mineralization. The studies suggest that the ABM/P-15 in hydrogels can be used as an injectable biomimetic matrix to facilitate bone repair.
Department of Bioengineering and The Center for Tissue Engineering, University of California, Berkeley, CA, USA.
Hieu
H
Nguyen
Jing Jing
JJ
Qian
Rajendra S
RS
Bhatnagar
Song
S
Li
D040761:Biomimetic Materials / Q000138:chemical synthesis; D001857:Bone Matrix / Q000502:physiology*; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003094:Collagen; D050723:Fractures, Bone / Q000628:therapy; D006801:Humans; D020100:Hydrogels; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010446:Peptide Fragments; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen