Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress.
Male broilers were used to evaluate the effect of zinc on performance and immune competence during heat stress (HS). Broilers raised in either a thermoneutral (TN, 23.9 degrees C constant) or HS (23.9 to 35 degrees C cycling) environment were fed a low zinc diet (LZ; 34 mg/kg), an adequate zinc diet (AZ; 68 mg/kg), or a supplemental zinc diet (HZ; 181 mg/kg). Humoral immunity was assessed by intravenous injection of 7% SRBC followed by evaluation of serum for antibody titers in primary and secondary responses. Cell-mediated immunity was assessed using a Sephadex stimulation method to recruit abdominal exudate cells (AEC) to evaluate macrophage phagocytic ability. The HS birds consumed 12.5% less feed, gained 24.6% less weight, and had lower feed efficiency when compared to TN birds. Dietary zinc levels did not impact growth performance or plasma zinc concentration. Numbers of AEC, macrophages in AEC, phagocytic macrophages, and internalized opsonized and unopsonized SRBC were increased by HZ. Total, IgM, and IgG antibody titers for primary and secondary responses were significantly increased in birds receiving HZ under TN conditions. Tibia zinc concentration increased with increasing zinc levels but did not change with temperature. Lymphoid organ weights, primary and secondary antibody responses, incidences of macrophages in AEC, phagocytic ability of macrophages, and plasma zinc concentration were all significantly reduced by HS. These results indicate that the immune response of broilers can be influenced by the level of zinc in the diet and by environmental conditions.
Bartlett
J R
JR
Department of Animal Science, The University of Tennessee, Knoxville, Tennessee 37996-4500, USA. jbartlett@tuskegee.edu
Smith
M O
MO
D000005:Abdomen; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens; D004435:Eating; D005122:Exudates and Transudates / Q000166:cytology; D018882:Heat Stress Disorders / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D006358:Hot Temperature; D007111:Immunity, Cellular; D007121:Immunocompetence / Q000187:drug effects*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008221:Lymphoid Tissue / Q000033:anatomy & histology; D008264:Macrophages / Q000276:immunology; D008297:Male; D009929:Organ Size; D010587:Phagocytosis; D011201:Poultry Diseases / Q000276:immunology / Q000503:physiopathology*; D013977:Tibia / Q000737:chemistry; D015430:Weight Gain; D015032:Zinc / Q000008:administration & dosage* / Q000032:analysis / Q000097:blood
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015032:Zinc