[Molecular analysis of alpha-galactosidase MEL genes from Saccharomyces sensu stricto].
To infer the molecular evolution of yeast Saccharomyces sensu stricto from analysis of the alpha-galactosidase MEL gene family, two new genes were cloned and sequenced from S. bayanus var. bayanus and S. pastorianus. Nucleotide sequence homology of the MEL genes of S. bayanus var. bayanus (MELb), S. pastorianus (MELpt), S. bayanus var. uvarum (MELu), and S. carlsbergensis (MELx) was rather high (94.1-99.3%), comparable with interspecific homology (94.8-100%) of S. cerevisiae MEL1-MEL11. Homology of the MEL genes of sibling species S. cerevisiae (MEL1), S. bayanus (MELb), S. paradoxus (MELp), and S. mikatae (MELj) was 76.2-81.7%, suggesting certain species specificity. On this evidence, the alpha-galactosidase gene of hybrid yeast S. pastorianus (S. carlsbergensis) was assumed to originate from S. bayanus rather than from S. cerevisiae.
State Research Center GosNIIgenetika, Moscow, 113545 Russia.
E S
ES
Naumova
I V
IV
Korshunova
G I
GI
Naumov
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012440:Saccharomyces / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D000519:alpha-Galactosidase / Q000235:genetics*
D017931:DNA Primers; D000519:alpha-Galactosidase