Extended-spectrum beta-lactamase-producing Enterobacteriaceae in community and private health care centers.
In 1999, 39 of 2,599 isolates of the family Enterobacteriaceae (1.5%) collected by eight private laboratories in the Aquitaine region in France produced an extended-spectrum beta-lactamase (ESBL). Among these were 19 Enterobacter aerogenes isolates; 8 Klebsiella pneumoniae isolates; 6 Escherichia coli isolates; 3 Proteus mirabilis isolates; and 1 isolate each of Serratia marcescens, Morganella morganii, and Providencia stuartii. ESBL producers were isolated from 38 patients, including 33 residents of 11 clinics or nursing homes and 5 ambulatory patients. Seven different ESBLs were characterized. These mainly consisted of TEM-24 (25 isolates) and TEM-21 (9 isolates), but TEM-15 (2 isolates) and TEM-3, TEM-19, SHV-4, and CTX-M-1 (1 isolate each) were also characterized. Seven strains showed the coexistence of different TEM- and/or SHV-encoding genes, including a new SHV-1 variant, SHV-44, defined by the substitution R205L previously reported for SHV-3 in association with S238G. The epidemiology of the ESBL producers was investigated by random amplification of polymorphic DNA, typing by enterobacterial repetitive intergenic consensus PCR, analysis of resistance cotransferred with the ESBL, and analysis of the restriction profiles of the ESBL-encoding plasmids. Of the TEM-24-expressing strains, 18 were E. aerogenes isolates, including 9 from the same clinic, that were representatives of the epidemic clone disseminating in France. Of the TEM-21-producing strains that belonged to different species of the family Enterobacteriaceae (E. coli, K. pneumoniae, and P. mirabilis), 8 were isolated in the same nursing home. Outbreaks due to strain and/or plasmid dissemination in these clinic and nursing home were demonstrated. The presence of ESBL producers in five ambulatory patients probably resulted from nosocomial acquisition. Our data highlight the serious need to monitor patients for ESBL-producing Enterobacteriaceae in general practice.
Laboratoire de Microbiologie, Université de Bordeaux 2, 33076 Bordeaux Cedex, France. corinne.arpin@bacterio.u-bordeaux2.fr
Corinne
C
Arpin
Véronique
V
Dubois
Laure
L
Coulange
Catherine
C
André
Isabelle
I
Fischer
Patrick
P
Noury
Frédéric
F
Grobost
Jean-Philippe
JP
Brochet
Jacqueline
J
Jullin
Brigitte
B
Dutilh
Gilberte
G
Larribet
Isabelle
I
Lagrange
Claudine
C
Quentin
D003001:Cloning, Molecular; D003151:Community Health Centers; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D005602:France / Q000453:epidemiology; D006801:Humans; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D009735:Nursing Homes; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis*
D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases