Activation of somatostatin receptor subtype 2 inhibits insulin secretion in the isolated perfused human pancreas.
OBJECTIVE
Five distinct somatostatin receptors (SSTRs) have been cloned, characterized, and designated SSTRs 1-5. The role of these receptors in B-cell signaling has not been well characterized.


METHODS
In the current study, the isolated perfused human pancreas model was used to determine the specific effect of 4 different somatostatin receptor agonists on insulin secretion.


CONCLUSIONS
We demonstrated that the SSTR 2 agonist and octreotide significantly suppressed insulin secretion. Furthermore, even during the immunoneutralization of endogenous intrapancreatic somatostatin, the SSTR 2 agonist was able to reverse the effect of somatostatin immunoneutralization by suppressing insulin secretion. These results demonstrate that activation of SSTR 2 suppresses insulin secretion in the isolated perfused human pancreas.
Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. cbrunica@bcm.tmc.edu
F Charles
FC
Brunicardi
Azmi
A
Atiya
Stefan
S
Moldovan
Timothy C
TC
Lee
Shawn P
SP
Fagan
Robert M
RM
Kleinman
Thomas E
TE
Adrian
David H
DH
Coy
John H
JH
Walsh
William E
WE
Fisher
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D008875:Middle Aged; D015282:Octreotide / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D017481:Receptors, Somatostatin / Q000819:agonists / Q000276:immunology / Q000502:physiology*; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; C112899:DC 32-87; D007140:Immunoglobulin Fab Fragments; D007328:Insulin; D017481:Receptors, Somatostatin; D013004:Somatostatin; C093733:somatostatin receptor 2; D015282:Octreotide