GmPAP3, a novel purple acid phosphatase-like gene in soybean induced by NaCl stress but not phosphorus deficiency.
Purple acid phosphatases (PAPs) are commonly found in plants, but the physiological functions of different classes of PAPs are not thoroughly understood. In the present study, we identified a novel gene, GmPAP3, from salt-stressed soybean using suppression subtractive hybridization (SSH) techniques. Protein sequence alignment studies and phylogenetic analysis strongly suggested that GmPAP3 belongs to the group of plant PAPs and PAP-like proteins that are distinct from those of fungi and animals. In addition, the invariable consensus metal binding residues of PAPs were all conserved in GmPAP3. Surprisingly, analysis of protein sorting signals showed that a putative mitochondrion targeting transit peptide is present on GmPAP3. Northern blot analysis revealed that NaCl stress causes a general induction of GmPAP3 expression in both roots and leaves of various cultivated (Glycine max) and wild (Glycine soja) soybean varieties. Further test using two genetically unrelated cultivated soybean varieties showed that the expression pattern of GmPAP3 is distinct from other PAP genes in soybeans. NaCl stress and oxidative stress but not phosphorus (P) starvation induces the expression of GmPAP3. These results suggest that the physiological role of GmPAP3 might be related to the adaptation of soybean to NaCl stress, possibly through its involvement in reactive oxygen species (ROS) forming and/or scavenging or stress-responding signal transduction pathways.
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong S.A.R., China.
Hong
H
Liao
Fuk-Ling
FL
Wong
Tsui-Hung
TH
Phang
Ming-Yan
MY
Cheung
Wing-Yen Francisca
WY
Li
Guihua
G
Shao
Xiaolong
X
Yan
Hon-Ming
HM
Lam
D000135:Acid Phosphatase / Q000235:genetics*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010758:Phosphorus / Q000172:deficiency*; D010802:Phylogeny; D018749:RNA, Plant / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology*; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary; D006023:Glycoproteins; D018749:RNA, Plant; D030262:Soybean Proteins; D010758:Phosphorus; D012965:Sodium Chloride; C091137:purple acid phosphatase; D000135:Acid Phosphatase