Novel radiosynthesis of PET HSV-tk gene reporter probes [18F]FHPG and [18F]FHBG employing dual Sep-Pak SPE techniques.
Positron emission tomography (PET) herpes simplex virus thymidine kinase (HSV-tk) gene reporter probes 9-[(3-[(18)F]fluoro-1-hydroxy-2-propoxy)methyl]guanine ([(18)F]FHPG) and 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl)guanine ([(18)F]FHBG) were prepared by nucleophilic substitution of the appropriate tosylated precursors with [(18)F]KF/Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified dual Silica Sep-Pak solid-phase extraction (SPE) method in 15-30% radiochemical yield.
Department of Radiology, Indiana University School of Medicine, 1345 West 16th Street, L-3 Room 202, Indianapolis, IN 46202-2111, USA.
Ji-Quan
JQ
Wang
Qi-Huang
QH
Zheng
Xiangshu
X
Fei
Bruce H
BH
Mock
Gary D
GD
Hutchins
D005462:Fluorine Radioisotopes; D015774:Ganciclovir / Q000031:analogs & derivatives; D017930:Genes, Reporter; D006147:Guanine / Q000031:analogs & derivatives / Q000138:chemical synthesis*; D007553:Isotope Labeling / Q000379:methods; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism*
C471398:9-(3-fluoro-1-hydroxy-2-propoxy)methylguanine; C113308:9-(4-fluoro-3-hydroxymethylbutyl)guanine; D005462:Fluorine Radioisotopes; D006147:Guanine; D013937:Thymidine Kinase; D015774:Ganciclovir