Cytotoxicity for porcine islet cells by complement of six animal species.
Complement-mediated cytotoxicity for porcine islet cells (PICs) was evaluated using sera of six animal species. Then soluble complement receptor type-1 (sCR1) as an anti-complement agent was added to those sera, and the changes in 50% hemolytic unit of complement serum (CH50) and cytotoxic effect of those sera on PICs were examined. All the sera except for that of pig showed cytotoxicity. However, the extent of toxicity was considerably different between species. In the rat and human serum, sCR1 significantly reduced CH50 and cytotoxicity, however in the dog serum, sCR1 had no suppressive effects. These results may suggest that complement contribute to humoral cytotoxicity for PICs as a main factor, and the compatibility of complement with PICs differs between animal species.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Shin
S
Itakura
Hisamune
H
Kato
Pi-Chao
PC
Wang
Atsuko
A
Matsunaga
Tomofumi
T
Jitsukawa
Kazuya
K
Edamura
Hisako
H
Ohgawara
Manabu
M
Mochizuki
Ryohei
R
Nishimura
Nobuo
N
Sasaki
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003165:Complement System Proteins / Q000633:toxicity*; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology*; D051381:Rats; D013045:Species Specificity; D013552:Swine
D003165:Complement System Proteins