Characterization of cellular phenotypes and cytokine expression in balt from children with congenital heart diseases.
The present study was performed to target and call attention to the bronchial associated lymphoid tissue (BALT), part of our immune system, from which, we believe, several forms of prophylactic and therapeutic approaches can be developed. The characterization of its immune components, cells, and cytokines, in absence of antigenic stimuli, is pioneer in literature. Eighteen cases of necropsies were chosen and selected the paraffin-embedded lungs. The ages of 11 females and 7 males varied from 5 to 31 months. Cause of death: congenital heart diseases.


METHODS
lung infection at necropsy and/or arterial hypertrophy greater than Heath-Edwards' 1st degree. Immunohistochemical technique was applied to identify the cell phenotypes and the cytokines in situ. BALT was identified in all cases in this study. The main cellular phenotypes in BALT were T helper (TH) and B lymphocytes surrounded by T cytotoxic lymphocytes, natural killer cells, and dendritic cells in less quantities. Interleukin 10 and Tumor Necrosis Factor alpha were the predominant cytokines in BALT without antigenic stimuli. BALT is an important structure of the lung immune system in infants, with a tendency to maintain an environment favorable to the Th2 arm of immune response. It needs more exploration to define its behavior in front of infections, especially those with pulmonary tropism.
Departamento de Patologia da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. talib@terra.com.br
T M
TM
Moussallem
F
F
Guedes
E R
ER
Fernandes
C
C
Pagliari
H F
HF
Andrade
M I S
MI
Duarte
C L P
CL
Lancellotti
D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006330:Heart Defects, Congenital / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008168:Lung / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000166:cytology*; D008221:Lymphoid Tissue / Q000166:cytology* / Q000378:metabolism; D008297:Male
D016207:Cytokines