Plasma protein adsorption of Tween 80- and poloxamer 188-stabilized solid lipid nanoparticles.
The plasma proteins adsorbing onto intravenously injected carriers are considered to be crucial factors determining the organ distribution. Plasma protein adsorption patterns were analyzed on solid lipid nanoparticles (SLN) stabilized with Tween 80 or stabilized with poloxamer 188. The binding patterns were determined by applying two different sample preparation methods, i.e. removal of the SLN from the plasma by (a) centrifugation and (b) gel filtration to assess, if the separation method has an effect on the patterns obtained. The Tween 80-modified SLN adsorbed the major plasma proteins known from particles with blood-brain barrier specificity. Poloxamer 188-surface modified SLN adsorbed the proteins known from model particles that exhibit prolonged circulation time in the blood. It is concluded that the biodegradable SLN stabilized with Tween 80 can potentially be used as drug carriers to the blood-brain barrier having a relatively long residence time in the blood stream. For the poloxamer 188-stabilized SLN a relatively long resistance time in the blood is predicted leading to potential accumulation in the bone marrow when looking at the distinct CII/CIII adsorption.
Department of Pharmaceutics, Biopharmaceutics and Quality Management, Institute of Pharmacy, The Free University of Berlin, Germany.
Torsten M
TM
Göppert
Rainer H
RH
Müller
D000327:Adsorption; D001798:Blood Proteins / Q000493:pharmacokinetics*; D006801:Humans; D008055:Lipids / Q000493:pharmacokinetics*; D036103:Nanotechnology / Q000379:methods*; D020442:Poloxamer / Q000493:pharmacokinetics*; D011136:Polysorbates / Q000493:pharmacokinetics*
D001798:Blood Proteins; D008055:Lipids; D011136:Polysorbates; D020442:Poloxamer