Role of nonclassical class I genes of the chicken major histocompatibility complex Rfp-Y locus in transplantation immunity.
The chicken major histocompatibility complex ( MHC) genes are organized into two genetically independent clusters which both possess class I and class IIbeta genes: the classical B complex and the Restriction fragment pattern- Y ( Rfp-Y) complex. In this study, we have examined the role of Rfp-Y genes in transplantation immunity. For this we used three sublines, B19H1, B19H2 and B19H3, derived from a line fixed for B19. Southern blots, PCR-SSCP assays using primers specific for Rfp-Y genes, and Rfp-Y class I allele-specific sequencing show that the polymorphisms observed in B19H1, B19H2 and B19H3 are due to the presence of three different Rfp-Y haplotypes. The Rfp-Y class I ( YF) alleles in these three haplotypes are highly polymorphic, and RT-PCR shows that at least two YF loci are expressed in each subline. The three sublines show Rfp-Y-directed alloreactivity in that Rfp-Y-incompatible skin grafts are rejected within 15 days, a rate intermediate between that seen in B-incompatible rejection (7 days) and that observed for grafts within the sublines (20 days). We conclude that Rfp-Y has an intermediate role in allograft rejection, likely to be attributable to polymorphism at the class I loci within this region.
Unité 086, Bioagresseurs, Santé, Environnement, INRA, Centre de Tours-Nouzilly, 37380, Nouzilly, France.
Pierrick
P
Thoraval
Marielle
M
Afanassieff
Danièle
D
Bouret
Gillette
G
Luneau
Evelyne
E
Esnault
Ronald M
RM
Goto
Anne-Marie
AM
Chaussé
Rima
R
Zoorob
Denis
D
Soubieux
Marcia M
MM
Miller
Ginette
G
Dambrine
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D003433:Crosses, Genetic; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006239:Haplotypes; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D016038:Skin Transplantation / Q000276:immunology; D014181:Transplantation Immunology