Quantitative analysis of 2-furfural and 5-methylfurfural in different Italian vinegars by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry using isotope dilution.
A new method was developed for the determination of 2-furfural (2-F) and 5-methylfurfural (5-MF), two products of Maillard reaction in vinegar, with head-space solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). A divinylbenzene (DVB)/carboxen (CAR)/polydimethylsiloxane (PDMS) fibre was used and SPME conditions were optimised, studying ionic strength effect, temperature effect and adsorption time. Both analytes were determined by calibration established on 2-furfural-d4 (2-F-d4). The method showed good linearity in the range studied (from 16 to 0.12 mg/l), with a regression coefficient r2 of 0.9999. Inter-batch precision and accuracy were found between 14.9 and 6.0% and between -11.7 and 0.2%, respectively. Detection limit was 15 microg/l. The method is simple and accurate and it has been applied to a series of balsamic and non-balsamic vinegars.
Giordano
Lucia
L
Gennaro Paone Environmental Health Center, Mario Negri Institute for Pharmacological Research, Consorzio Mario Negri Sud, Via Nazionale, 66030 Santa Maria Imbaro, Chieti, Italy.
Calabrese
Roberto
R
Davoli
Enrico
E
Rotilio
Domenico
D
D019342:Acetic Acid / Q000737:chemistry*; D002138:Calibration; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007554:Isotopes; D012492:Salts; D013696:Temperature
D007554:Isotopes; D012492:Salts; C048065:5-methyl-2-furfural; D005662:Furaldehyde; D019342:Acetic Acid