A modified restriction display PCR method in sample-labelling of DNA microarray.
The restriction display PCR is a useful technique for studying the diversity of gene expression. This method involves ligating the digested genes with adapters and amplifying the gene fragments by PCR using universal and selective primers. In this study, we improved this restriction display PCR method by using Cy3-UP, a fluorescently labelled universal primer, in place of Cy3-dCTP in sample-labelling for DNA microarray. The results show that this new method increases significantly the sensitivity of the assay, and will have a wide application in the DNA microarray field.
Li
Ling
L
Institute of Molecular Biology, The First Uniform Medical University, Guangzhou 510515, China. liling@fimmu.com
Ma
Wen Li
WL
Zhu
Ji
J
Shi
Rong
R
Liu
Cui Hua
CH
Chen
Jin Kun
JK
Zheng
Wen Ling
WL
D002232:Carbocyanines / Q000378:metabolism*; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D005456:Fluorescent Dyes / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods
D002232:Carbocyanines; D017931:DNA Primers; D004279:DNA, Viral; D005456:Fluorescent Dyes; C089187:cyanine dye 3