Increased gene expression of beta-1,4-galactosyltransferase I in rat injured sciatic nerve.
During neurite outgrowth on basal lamina, cell-surface beta-1,4-galactosyltransferase I (beta-1,4-GalT-I) functions as one of the receptors of laminin by binding to N-linked oligosaccharides on the laminin E8 domain. In the present study, it was revealed that in rat injured sciatic nerves, the expression of beta-1,4-GalT-I mRNA reached its peak 2-3 d after axotomy in both proximal and distal stumps, and decreased thereafter as demonstrated by Northern blot analysis. In situ hybridization revealed that beta-1,4-GalT-I mRNAmainly localized in Schwann cells of the injured nerves. Moreover the Galbeta1-4GlcNAc (N-acetylglucosamine) group mainly localized in Schwann cells of the injured nerves by Ricinus communis agglutinin-I (RCA-I) lectin histochemistry. However, the changes in abundance of the Galbeta1-4GlcNAc group in injured nerves were not consistent with the expression of beta-1, 4-GalT-I mRNA. These findings indicate that beta-1,4-GalT-I might be involved in the regeneration of injured peripheral nerves at the early injury stage.
Shen
Aiguo
A
The Jiangsu Province Key Lab of Neuroregeneration, Nantong Medical College, Nantong 226001, People's Republic of China.
Zhu
Dan
D
Ding
Fei
F
Zhu
Min
M
Gu
Xiaosong
X
Gu
Jianxin
J
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D020439:Growth Cones / Q000201:enzymology / Q000648:ultrastructure; D007797:Laminin / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000201:enzymology* / Q000503:physiopathology; D037121:Plant Lectins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000235:genetics; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000201:enzymology* / Q000503:physiopathology; D015854:Up-Regulation / Q000235:genetics*
D007797:Laminin; D037121:Plant Lectins; D012333:RNA, Messenger; C017707:Ricinus communis agglutinin-1; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I