Correlation between pyrazinamide activity and pncA mutations in Mycobacterium tuberculosis isolates in Taiwan.
A total of 76 clinical Mycobacterium tuberculosis isolates from Taiwan were tested for pyrazinamidase activity, pyrazinamide susceptibility, and pncA mutations. Frequency of resistance to PZA rose with increases in resistance to first-line drugs. Of 17 pyrazinamide-resistant strains, 7 (3 of which had not been previously described) possessed mutations in the pncA gene.
Huang
Tsi-Shu
TS
Section of Microbiology and Infectious Diseases, Kaohsiung Veterans General Hospital and Department of Medical Technology, Foo-Yin Institute of Technology, Taipei, Taiwan.
Lee
Susan Shin-Jung
SS
Tu
Hui-Zin
HZ
Huang
Wen-Kuei
WK
Chen
Yao-Shen
YS
Huang
Chung-Kai
CK
Wann
Shue-Ren
SR
Lin
Hsi-Hsun
HH
Liu
Yung-Ching
YC
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D000995:Antitubercular Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005838:Genotype; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D011718:Pyrazinamide / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan; D014376:Tuberculosis / Q000382:microbiology*
D000995:Antitubercular Agents; D004269:DNA, Bacterial; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis; C022490:pyrazinamide deamidase