Electron microscopic observation of 137Cs-irradiated rat testis: production of basal laminae for germ cells, despite their absence.
Whole body gamma-ray irradiation of rats with caesium-137 (137Cs) at embryonic day 20 induced marked reduction of the weight of the testis. Body weight and other tissues, however, seemed to remain normal. By light microscopy, complete loss of germ cells was observed in the testis. Other components, such as Sertoli cells and interstitial cells, seemed to be normal. The testes from day 8 postpartum rats contained very few spermatogonia compared with newborn rats, indicating loss of germ cells between days 0 and 8. In the adult, 137Cs-irradiated testes showed two conspicuous features other than the loss of germ cells: empty vacuolar spaces between Sertoli cells and multilayered seminiferous tubule basal laminae (lamina densa). The junctional structures (ectoplasmic specializations) between Sertoli cells, however, seemed normal. The thickness of each layer of multilayered basal laminae was the same as that of normal rats and electron-lucent layers similar to lamina lucida were interposed between them. Of the empty vacuolar spaces between Sertoli cells, basal laminae bridge the gap. The basal laminae contained laminin, type IV collagen and heparan sulphate proteoglycan evenly distributed among layers, suggesting a normal composition. Rough estimation of the amount of basal laminae deposited in 137Cs-irradiated rats indicates that it is within a range similar to that in normal testis. These features imply that Sertoli cells are, in part, determined perinatally to produce basal laminae for germ-line cells.
Department of Anatomy, Yokohama City University School of Medicine, Fukuura 3-9, Kanazawa-ku, Yokohama 236, Japan. hsawada@med.yokohama-cu.ac.jp
Hajime
H
Sawada
Michiyo
M
Esaki
D000818:Animals; D000831:Animals, Newborn; D001485:Basement Membrane / Q000528:radiation effects / Q000648:ultrastructure; D002588:Cesium Radioisotopes; D005260:Female; D005854:Germ Cells / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D012671:Seminiferous Tubules / Q000528:radiation effects / Q000648:ultrastructure; D012708:Sertoli Cells / Q000528:radiation effects / Q000648:ultrastructure; D013737:Testis / Q000196:embryology / Q000528:radiation effects* / Q000648:ultrastructure*; D014916:Whole-Body Irradiation
D002588:Cesium Radioisotopes