Expression of kynurenine aminotransferases in the rat retina during development.
The study investigates the cellular expression of kynurenine aminotransferases (KAT I and II) in the rat retina during development. At P1 (the day of birth) and P7 (the 7th day after birth), KAT I expression was observed in the inner plexiform layer (IPL), the fiber layer (FL), and in vertically running processes in the ganglion cell layer (GCL) (but not in the cell bodies). At P14 (the 14th day after birth) a strong KAT I immunoreactivity was observed in Müller cell endfeet. KAT II was expressed in the IPL, the FL, and in cells in the GCL at P1 and P7. From P14 on, KAT II expression in the IPL decreased. Double labeling revealed that KAT I was expressed in Müller cell endfeet, whilst KAT II both on retinal ganglion cells (RGC) and Müller cell endfeet. In conclusion, KAT I and II are present in the rat retina during development. The heterogeneity of the KAT developmental profiles possibly reflects a neuromodulatory role in the retinal differentiation.
Department of Pathophysiology of Vision and Neuro-Ophthalmology, University Eye Hospital, Division of Experimental Ophthalmology, Röntgenweg 11, D-72076 Tübingen, Germany. rrejdak@yahoo.com
Robert
R
Rejdak
Yana
Y
Shenk
Frank
F
Schuettauf
Waldemar A
WA
Turski
Etsuo
E
Okuno
Zbigniew
Z
Zagorski
Eberhart
E
Zrenner
Konrad
K
Kohler
D000818:Animals; D005453:Fluorescence; D007150:Immunohistochemistry; D051381:Rats; D012160:Retina / Q000201:enzymology* / Q000254:growth & development; D000637:Transaminases / Q000378:metabolism*
D000637:Transaminases; C021481:kynurenine-oxoglutarate transaminase