Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na+ transport.
Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Yasuhiro
Y
Kato
Akihiro
A
Hazama
Mutsumi
M
Yamagami
Nobuyuki
N
Uozumi
D000818:Animals; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D004594:Electrophysiology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000494:pharmacology; D015335:Molecular Probes / Q000494:pharmacology*; D009842:Oligopeptides; D009865:Oocytes; D010455:Peptides / Q000494:pharmacology; D010940:Plant Proteins; D012964:Sodium / Q000378:metabolism; D027981:Symporters; D014981:Xenopus
D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; C490286:HKT1 protein, Arabidopsis; C088864:HKT1 protein, plant; D008164:Luminescent Proteins; D015335:Molecular Probes; D009842:Oligopeptides; D010455:Peptides; D010940:Plant Proteins; D027981:Symporters; D049452:Green Fluorescent Proteins; C085706:FLAG peptide; D012964:Sodium