Screening and mutagenesis of Aspergillus niger for the improvement of glucose 6-phosphate dehydrogenase production.
The strain of Aspergillus niger ZBY-7 was selected as the original strain of glucose 6-phosphate dehydrogenase production. After mutagenesis of the strain using UV irradiation and nitrosoguanidine, mutants of Aspergillus niger resistant to certain metabolic inhibitor were obtained. Five of the mutants showed increased glucose 6-phosphate dehydrogenase production. The mutant resistant to antimycin A (Aspergillus niger AM-23) produced the highest level of glucose 6-phosphate dehydrogenase (695.9% of that from the original strain).
Liu
Jian-Zhong
JZ
Key Laboratory of Gene Engineering of Ministry of Education and Biotechnology Research Center, Zhongshan University, Guangzhou 510275, P.R. China.
Zhang
Qian-Ling
QL
Weng
Li-Ping
LP
Ji
Liang-Nian
LN
D000935:Antifungal Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics*; D026141:Drug Resistance, Multiple, Fungal / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D007218:Industrial Microbiology; D016296:Mutagenesis / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation; D009604:Nitrosoguanidines; D014466:Ultraviolet Rays; D015854:Up-Regulation
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D009604:Nitrosoguanidines; D000968:Antimycin A; D005954:Glucosephosphate Dehydrogenase