Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis).
A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.
School of Agriculture and Wine, The University of Adelaide, Waite Campus, PMB #1, Glen Osmond, SA 5064, Australia.
Genet
G
Mekuria
Sunita A
SA
Ramesh
Evita
E
Alberts
Terry
T
Bertozzi
Michelle
M
Wirthensohn
Graham
G
Collins
Margaret
M
Sedgley
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019179:Ilarvirus / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D012367:RNA, Viral