[The effects of lactated ringer's resuscitation on tissue blood flows and oxidant injury in experimental crush injury in rats].
BACKGROUND
In an experimental model of crush injury, tissue blood flow, the extend of oxidant injury and the effect of lactated ringer's resuscitation were investigated.


METHODS
Rats were divided into sham (n: 8), crush injury (n: 8), and crush injury + lactated ringer's resuscitation (n: 8) groups. Arterial and venous catheterization were performed in all groups. Crush injury was done with intravenous infusion of allogenic muscle extract. In the crush injury + lactated ringer's resuscitation group 30 ml/kg lactated ringer's solution was infused in 30 minutes. Kidney and liver blood flow were measured by using a laser flowmeter. To assess the oxidant injury blood, liver, and kidney tissue samples were collected for malondialdehyde and glutathione measurements.


RESULTS
In the crush injury, diminished liver and kidney blood flow rates were improved with lactated ringer's resuscitation. In addition, glutathione levels decreased whereas malondialdehyde levels and base deficit increased. Lactated ringer's resuscitation brought base deficit to the control levels. When compared with the crush injury, lactated ringer's infusion increased the glutathione levels but could not decrease the malondialdehyde ones.


CONCLUSIONS
Lactated ringer's resuscitation improved the blood flow rates but could not prevent oxidant injury totally.
Uludag University, Faculty of Medicine, Department of Emergency Medicine, Bursa, Turkey. dr_veyselbalci@yahoo.com
Veysel
V
Balci
Halil
H
Ozgüç
Sule
S
Akköse
Nevzat
N
Kahveci
Zehra
Z
Serdar
Rifat
R
Tokyay
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D007552:Isotonic Solutions / Q000627:therapeutic use*; D007668:Kidney / Q000098:blood supply / Q000378:metabolism; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000098:blood supply / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D011673:Pulsatile Flow; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000628:therapy*
D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D008315:Malondialdehyde; D005978:Glutathione