The two rotor components of yeast mitochondrial ATP synthase are mechanically coupled by subunit delta.
The mitochondrial ATP synthase is made of a membrane-integrated F0 component that forms a proton-permeable pore through the inner membrane and a globular peripheral F1 domain where ATP is synthesized. The catalytic mechanism is thought to involve the rotation of a 10-12 c subunit ring in the F0 together with the gamma subunit of F1. An important and not yet resolved question is to define precisely how the gamma subunit is connected with the c-ring. In this study, using a doxycycline-regulatable expression system, we provide direct evidence that the rest of the enzyme can assemble without the delta subunit of F1, and we show that delta-less mitochondria are uncoupled because of an F0-mediated proton leak. Based on these observations, and taking into account high-resolution structural models, we propose that subunit delta plays a key role in the mechanical coupling of the c-ring to subunit gamma.
Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Université Victor Segalen, 1 Rue Camille Saint-Saëns, Bordeaux 33077 Cedex, France.
Stéphane
S
Duvezin-Caubet
Matthieu
M
Caron
Marie-France
MF
Giraud
Jean
J
Velours
Jean-Paul
JP
di Rago
D000251:Adenosine Triphosphatases / Q000378:metabolism; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry*; D010101:Oxygen Consumption; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013997:Time Factors
D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D004318:Doxycycline