Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins.
Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), a recently described disease of young pigs. Since no PCV2 vaccine was available so far, we have developed a specific PCV2 vaccine candidate. The Orf1-encoded replication protein and Orf2-encoded capsid protein of PCV2 were expressed and detected in either mammalian or insect expression systems. In a first trial, Orf2 protein was found to be a major immunogen, inducing protection in a prime-boost protocol; the piglets received a first injection with plasmids directing Orf2 protein and granulocyte-macrophage colony-stimulating factor (GM-CSF) expression, followed by a second injection, a fortnight later, associated with baculovirus-expressed Orf2 protein. As evaluated by growth parameters, clinical signs (fever), seroconversion, the pigs were protected against a PCV2 challenge after vaccination. In a second trial, protection induced by a subunit vaccine was even better than the one induced by DNA vaccine, since PCV2 replication was completely inhibited.
Blanchard
P
P
Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Ploufragan, Unité Génétique Virale et Biosécurité, BP 53, Zoopôle Les Croix, Fr 22440 Ploufragan, France. p.blanchard@ploufragan.afssa.fr
Mahé
D
D
Cariolet
R
R
Keranflec'h
A
A
Baudouard
M A
MA
Cordioli
P
P
Albina
E
E
Jestin
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D018173:Circoviridae Infections / Q000503:physiopathology / Q000517:prevention & control* / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005334:Fever / Q000503:physiopathology / Q000517:prevention & control; D007117:Immunization, Secondary; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D015430:Weight Gain / Q000502:physiology
D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines