[Definition of neurotoxic risk based on the benchmark dose in vitro: illustration with experimental models based on the use of known neurotoxic substances].
The BMD approach has been used to compare the cell viability (MTT assay) of different rat (C6 and PC12, glial and neuronal, respectively) and human cell lines (D384 and SK-N-MC, glial and neuronal, respectively) after 24-h exposure to the following neurotoxic substances: Manganese Chloride (MnCl2), Methyl-mercury (Me-Hg) and the enantiomers of Styrene Oxide (SO). For all rat and human cell lines, the potency of the examined compounds was: MnCl2 < S-SO < R-SO < Me-Hg. A preliminary comparison with in vivo toxicity data for these substances gave rise to consistent results. Whereas a reasonable agreement between in vitro and in vivo data has been found for Mn and styrene oxide, a wide scatter of LOAEL has been reported for Me-Hg and these appear to be either much higher or lower than the BMD for the MTT assay we observed in vitro.
Vettori
M V
MV
Centro Studi e Ricerche ISPESL, Laboratorio di Tossicologia Industriale dell'Università di Parma, Parma.
Goldoni
M
M
Caglieri
A
A
Palmi
S
S
Ursini
L C
LC
Mutti
A
A
D000818:Animals; D002273:Carcinogens / Q000009:adverse effects*; D002460:Cell Line; D002712:Chlorides / Q000009:adverse effects*; D004852:Epoxy Compounds / Q000009:adverse effects*; D006801:Humans; D017895:Manganese Compounds / Q000009:adverse effects*; D008767:Methylmercury Compounds / Q000009:adverse effects*; D020258:Neurotoxicity Syndromes / Q000209:etiology*; D051381:Rats; D018570:Risk Assessment; D020711:Threshold Limit Values
D002273:Carcinogens; D002712:Chlorides; D004852:Epoxy Compounds; D017895:Manganese Compounds; D008767:Methylmercury Compounds; C013690:styrene oxide; C025340:manganese chloride