Neuronal migration depends on intact peroxisomal function in brain and in extraneuronal tissues.
Functional peroxisome deficiency, as encountered in Zellweger syndrome, causes a specific impairment of neuronal migration. Although the molecular mechanisms underlying the neuronal migration defect are at present unknown, the excess of very long chain fatty acids in brain, a consequence of peroxisomalbeta-oxidation deficiency, has often been hypothesized to play a major role. The purpose of the present study was to investigate the contribution of peroxisomal dysfunction in brain as opposed to peroxisomal dysfunction in extraneuronal tissues to the migration defect. Peroxisomes were selectively reconstituted either in brain or liver of Pex5 knock-out mice, a model for Zellweger syndrome, by tissue-selective overexpression of Pex5p. We found that both rescue strains exhibited a significant correction of the neuronal migration defect despite an incomplete reconstitution of peroxisomal function in the targeted tissue. Animals with a simultaneous rescue of peroxisomes in both tissues displayed a pattern of neuronal migration indistinguishable from that of wild-type animals on the basis of cresyl violet staining and 5',3'-bromo-2'-deoxyuridine birth-dating analysis. These data suggest that peroxisomal metabolism in brain but also in extraneuronal tissues affects the normal development of the mouse neocortex. In liver-rescued mice, the improvement of the neuronal migration was not accompanied by changes in very long chain fatty acid, docosahexaenoic acid, or plasmalogen levels in brain, indicating that other metabolic factors can influence the neuronal migration process.
Janssen
Anneleen
A
Laboratory of Clinical Chemistry, K. U. Leuven, 3000 Leuven, Belgium.
Gressens
Pierre
P
Grabenbauer
Markus
M
Baumgart
Eveline
E
Schad
Arno
A
Vanhorebeek
Ilse
I
Brouwers
Annelies
A
Declercq
Peter E
PE
Fahimi
Dariush
D
Evrard
Philippe
P
Schoonjans
Luc
L
Collen
Désiré
D
Carmeliet
Peter
P
Mannaerts
Guy
G
Van Veldhoven
Paul
P
Baes
Myriam
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D007381:Intermediate Filament Proteins / Q000235:genetics; D008099:Liver / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins; D064231:Nestin; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009928:Organ Specificity / Q000235:genetics; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D000074435:Peroxisome-Targeting Signal 1 Receptor; C000614265:Pex5 protein, mouse; D018160:Receptors, Cytoplasmic and Nuclear; D001973:Bromodeoxyuridine