Biostability and biocompatibility of a surface-grafted phospholipid monolayer on a solid substrate.
We have previously demonstrated phosphorylcholine monolayer chemically grafted onto a methacryloyl-terminated solid substrate by in situ polymerization. The in situ polymerization was carried out at the interface between a pre-assembled acrylated phospholipid monolayer produced by vesicle fusion and a methacryloyl-terminated substrate using a water-soluble initiator, 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPD). Herein, we examined the biostability and biocompatibility of a surface-grafted phospholipid monolayer (poly-PC) on a methacryloyl-terminated substrate using a "wash off' test, in vitro protein adsorption and in vivo cage implantation for time intervals of 4, 7, 14 and 21 days, respectively. In order to compare the biostability and biocompatibility of phospholipid surfaces on solid substrates, we used two types of phospholipid surfaces: a physically adsorbed phospholipid monolayer (PC) and a poly-PC. Atomic force microscopy and water contact angle measurements indicated that the poly-PC surface was more stable in PBS, Triton X-100 and to EO gas sterilization than the PC surface. The adsorption of proteins such as albumin, fibrinogen, IgG and human plasma proteins on the poly-PC surfaces were significantly reduced, in vitro. Moreover, the poly-PC surface greatly reduced macrophage adhesion and the formation of foreign body giant cells, in vivo.
Department of Materials Science and Engineering, Kwangju Institute of Science and Technology, 1 Oryong-dong, Puk-gu, Gwangju 500-712, South Korea.
Kwangmeyung
K
Kim
Chulhee
C
Kim
Youngro
Y
Byun
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000648:ultrastructure; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019544:Equipment Failure Analysis; D005549:Foreign-Body Reaction / Q000209:etiology / Q000473:pathology*; D015743:Giant Cells, Foreign-Body / Q000473:pathology*; D006801:Humans; D008264:Macrophages / Q000473:pathology*; D008422:Materials Testing; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry*; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D010743:Phospholipids