Spectroscopic study of antileishmanial drug incubated in the promastigotes of Leishmania mexicana.
In this work we present spectroscopic study of Boldine (aporphine alkaloid) that possesses important biological activities, in particular, in interaction with the promastigotes of Leishmania mexicana. The results show the applicability of autofluorescence of this drug to determinate the possible mechanism of its biological action. The blue shift and hyperchromic effect in the emission spectrum of the drug in interaction with the parasite cells indicate an energy transference process between them. The morphological change of cell shape of the promastigotes treated with the drug is observed using confocal microscopy. This morphological cell-shape transformation evidences an important interaction between the drug studied and some protein of the parasite cell. Here we describe for the first time the fluorescence properties of the Boldine in the promastigotes of L. mexicana.
Laboratorios de Espectroscopia Láser, Facultad de Ciencias, Escuela de Química, Universidad Central de Venezuela, PO Box 47102, Caracas 1020A, Venezuela.
J
J
Hung
J
J
Castillo
G
G
Jiménez
M
M
Hasegawa
M
M
Rodriguez
D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000494:pharmacology*; D001060:Aporphines / Q000737:chemistry / Q000494:pharmacology; D007894:Leishmania mexicana / Q000166:cytology / Q000187:drug effects*; D018613:Microscopy, Confocal; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
D000981:Antiprotozoal Agents; D001060:Aporphines; C011686:boldine