Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.
The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium. amartin@itg.be
Anandi
A
Martin
Mirtha
M
Camacho
Françoise
F
Portaels
Juan Carlos
JC
Palomino
D000995:Antitubercular Agents / Q000494:pharmacology*; D003124:Colorimetry; D024881:Drug Resistance, Bacterial; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010078:Oxazines; D015203:Reproducibility of Results; D014966:Xanthenes
D000995:Antitubercular Agents; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin