Strategies to improve protection against low-pathogenicity H7 avian influenza virus infection using DNA vaccines.
Eukaryotic expression plasmids encoding either the avian influenza hemagglutinin or matrix genes (pCMV-HA and pCMV-M, respectively) were constructed. The viral genes were derived from a low-pathogenicity H7N1 strain, A/Chicken/Italy/1067/99, isolated during the 1999-2001 epizootic in Italy. The plasmid was administered to 4-to-5-wk-old specific-pathogen-free chickens by several different injection methods. For the initial studies comparing methods of vaccine injection, results were compared based on hemagglutination inhibition (HI) response following immunization with pCMV-HA. Additional studies with coadministration of both pCMV-HA and pCMV-M was evaluated based on HI response and viral isolation after homologous challenge. Preliminary results indicate that a device intended to inject insulin in humans (Medijector) and the coadministration of both plasmids improved protection against H7 infection.
AFSSA-Site de Ploufragan, Avian and Rabbit Virology, Immunology and Parasitology Unit, BP 53, Zoopole, 22440 Ploufragan, France.
M
M
Cherbonnel
J
J
Rousset
V
V
Jestin
D000818:Animals; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000008:administration & dosage / Q000276:immunology; D005814:Genes, Viral; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D009980:Influenza A virus / Q000302:isolation & purification / Q000472:pathogenicity*; D007252:Influenza Vaccines; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control; D007558:Italy; D010957:Plasmids; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D014162:Transfection; D019444:Vaccines, DNA
D018076:DNA, Complementary; D004279:DNA, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D019444:Vaccines, DNA