Identifying a region of interest in site- and stage-specific colon cancer on chromosome 13.
BACKGROUND
The role of genes on chromosome 13q has not been confirmed in colorectal tumors, in part because most series that have been studied are heterogeneous in terms of tumor site, stage, and replication error (RER) status. Using a highly homogenous series of colon tumors, our aim was to identify areas of interest on 13q that are important in carcinogenesis.


METHODS
Twenty-three RER-negative tumor specimens from patients with right-sided Dukes' stage C colon tumors were selected for analysis with 10 microsatellite markers spanning 13q. The polymerase chain reaction-amplified products were analyzed by using a standard fluorescent loss of heterozygosity/allele imbalance assay.


RESULTS
Markers showing the highest frequency of allelic imbalance were as follows: D13S175 (31%), D13S289 (27%), D13S263 (25%), and D13S265 (27%). The overall resolution of the map was approximately 11.4 to 11.7 cM.


CONCLUSIONS
This study of right-sided, RER-negative, Dukes' stage C colon tumors showed the highest area of allelic imbalance corresponding to 13q11.2-11. This region includes LATS2 (large tumor suppressor 2 gene) and FGF9 (fibroblast growth factor 9), which may be involved in carcinogenesis.
Sivarajasingham
Nala S
NS
Academic Surgical Unit, Division of Cancer, Postgraduate Medical Institute of the University of Hull, in association with the Hull and York Medical School, Castle Hill Hospital, Castle Road, Cottingham, United Kingdom.
Baker
Richard
R
Tilsed
Jonathan V
JV
Greenman
John
J
Monson
John R T
JR
Cawkwell
Lynn
L
D000483:Alleles; D002471:Cell Transformation, Neoplastic; D002882:Chromosomes, Human, Pair 13; D015331:Cohort Studies; D003110:Colonic Neoplasms / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction