LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma.
A liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric method (LC-APCI-MS-MS) for the determination of glybenclamide in human plasma is described. Glypizide, an analogue of glybenclamide, was used as internal standard. The analyte was extracted from plasma with diethyl ether/dichloromethane (70:30 v/v). The chromatography uses C18 and 0.01 mol L(-1) acetic acid/acetonitrile (20:80 v/v) as stationary and mobile phase, respectively. Quantitation was preformed by using multiple reaction monitoring (MRM) of the precursor ion (m/z 494.2-->368.8) and the related product ion (m/z 446.0-->347.3) using the internal standard method. The analytical curve was linear in the range 1-300 ng mL(-1), and for a 400-microL sample of human plasma, the limit of determination of the method was 1 ng mL(-1). The coefficients of variation of the method for intra-assay (within-run precision) and inter-assay (between-run precision) were less than 10%. The method was shown to be suitable for pharmacokinetic studies.
Department of Food Science, State University of Campinas, PO Box 6121, CEP 13.083-970 Campinas, SP, Brazil.
Mirian R L
MR
Moura
Gilberto
G
de Nucci
Susanne
S
Rath
Felix G R
FG
Reyes
D002853:Chromatography, Liquid / Q000379:methods*; D005905:Glyburide / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005905:Glyburide