Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
Because serum albumin is specifically produced by mature hepatocytes, detection system of albumin producing cells could be a valuable tool to visualize liver regeneration or development. We have developed here an albumin enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having liver-specific reporter gene expression of red fluorescent protein. To study the transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver damage by repeated administration of CCl(4). DsRed2-positive cells were generated in the recipient liver after BMC injection. The number of transdifferentiated DsRed2-positive cells in chronic liver injury model was increased comparing with that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited to studying in vivo liver regeneration.
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi, Japan.
Yuki
Y
Sato
Yuka
Y
Igarashi
Yoji
Y
Hakamata
Takashi
T
Murakami
Takashi
T
Kaneko
Masafumi
M
Takahashi
Norimasa
N
Seo
Eiji
E
Kobayashi
D000818:Animals; D030801:Animals, Genetically Modified; D016026:Bone Marrow Transplantation; D017690:Cell Transplantation; D006521:Hepatitis, Chronic / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D008099:Liver / Q000196:embryology* / Q000378:metabolism* / Q000601:surgery; D008115:Liver Regeneration; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D051381:Rats / Q000235:genetics; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism*
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin; C413663:fluorescent protein 583