[A new method for amplification and sequence analysis of the full-length of HBV genome].
OBJECTIVE
To develop a new method for amplifying and sequencing the full-length of HBV genome.


METHODS
A pair of primers located at the nick region of HBV molecule and a thermostable polymerase with high fidelity and sensitivity were used. After cloning the PCR products into a plasmid, the sequences of HBV genome were analyzed.


RESULTS
The full-length of HBV genome were acquired using this method. The sensitivity and fidelity of the new method were also analyzed. The least quantity of initial templates was 10(2) and the artificial mutation rate was 1.2 bp/kb.


CONCLUSIONS
This method can be used in amplification and sequence analysis of the full-length of HBV genome on a large scale.
Department of Infectious Diseases, Heping Hospital of Changzhi Medical College, Changzhi 046000, China.
Jin-jun
JJ
Guo
Ai-long
AL
Huang
Zhong-fu
ZF
Zhao
Ni
N
Tang
Hui-min
HM
Shen
D017931:DNA Primers / Q000235:genetics; D005784:Gene Amplification; D016679:Genome, Viral; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014771:Virion / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D017931:DNA Primers