Effects of C1 esterase inhibitor administration on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation during experimental endotoxemia.
OBJECTIVE
To determine the effects of C1 esterase inhibitor (C1-INH) administration on intestinal functional capillary density, leukocyte adherence, and mesenteric plasma extravasation during experimental endotoxemia.


METHODS
Prospective, randomized, controlled animal study in the experimental laboratory of a university.


METHODS
42 male Wistar rats.


METHODS
The animals were divided into three groups. One half of the animals of each group underwent studies of intestinal functional capillary density and leukocyte adherence on venular endothelium by intravital fluorescence microscopy. In the other half of the animals mesenteric plasma extravasation (FITC albumin) was determined by intravital fluorescence microscopy. Treatment groups received endotoxin infusion of 2.5 mg/kg per hour (group 2 and 3) and 100 U/kg b.w. C1-INH (group 3) during the 2 h of endotoxemia.


RESULTS
Endotoxemia resulted in a significant decrease in mucosal functional capillary density (18.5% vs. controls), which was reduced by C1-INH administration (9.5%). Treatment with C1-INH also significantly attenuated intestinal leukocyte adherence in submucosal venules (35% vs. endotoxin group) and mesenteric plasma extravasation (44% vs. endotoxin group).


CONCLUSIONS
C1-INH administration diminishes endotoxin-induced changes in the intestinal microcirculation during experimental endotoxemia.
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, F.-Loeffler-Strasse 23, 17489, Greifswald, Germany. christian.lehmann@uni-greifswald.de.|Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany. christian.lehmann@uni-greifswald.de.
Christian
C
Lehmann
Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Jürgen
J
Birnbaum
Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Carsten
C
Lührs
Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Oskar
O
Rückbeil
Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Claudia
C
Spies
Insitut für Laboratoriumsmedizin und Pathobiochemie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Sabine
S
Ziemer
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, F.-Loeffler-Strasse 23, 17489, Greifswald, Germany.
Matthias
M
Gründling
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, F.-Loeffler-Strasse 23, 17489, Greifswald, Germany.
Dragan
D
Pavlovic
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, F.-Loeffler-Strasse 23, 17489, Greifswald, Germany.
Taras
T
Usichenko
Klinik für Anästhesiologie und Intensivmedizin, Ernst-Moritz-Arndt-Universität Greifswald, F.-Loeffler-Strasse 23, 17489, Greifswald, Germany.
Michael
M
Wendt
Klinik für Anaesthesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstrasse 20/21, 10117, Berlin, Germany.
Wolfgang J
WJ
Kox
D000818:Animals; D002196:Capillaries / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000097:blood; D003174:Complement C1 Inactivator Proteins / Q000494:pharmacology*; D019446:Endotoxemia / Q000188:drug therapy* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000098:blood supply / Q000187:drug effects; D008297:Male; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D015815:Cell Adhesion Molecules; D003174:Complement C1 Inactivator Proteins