Artificial carrier for oxygen supply in biological systems.
Several poly (dimethylsiloxanes) (PDMS) copolymers of dimethylsiloxane (DMS) with ethylene or propylene oxide were tested as artificial carriers for the delivery of oxygen to biological systems. Copolymers with a DMS content of 33% or lower enhanced glucose oxidation by 200% in contrast to the 25% increase produced by the same concentration of perfluorodecalin. When 0.05% of the copolymer with 18% DMS was included in the growth media of Bacillus thuriginensis, the biomass (growth rate) increased 1.5-fold. With 0.1% of this copolymer, actinorhodin production by Streptomyces coelicolor A3 (2) occurred in half the normal time and with an increased yield. In conclusion, these PDMS copolymers are a good alternative to perfluorodecalin as oxygen carriers in biotechnological processes.
Pure and Applied Biochemistry, Lund University, Box 124, 212 00, Lund, Sweden. estera.dey@tbiokem.lth.se
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D000880:Anthraquinones / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry; D005466:Fluorocarbons / Q000737:chemistry / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D015394:Molecular Structure; D009757:Nylons / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D004129:Dimethylpolysiloxanes; D004852:Epoxy Compounds; D005027:Ethylene Oxide; D005466:Fluorocarbons; D005947:Glucose; D009757:Nylons; D010100:Oxygen; C085707:poly(dimethylsiloxane)-polyamide copolymer; C008805:perfluorodecalin; C013390:actinorhodin; D000095622:Benzoisochromanequinones; C009068:propylene oxide