Role of dopamine D2 receptors in the striatal immediate early gene response to amphetamine in reserpinized rats.
The indirect dopamine agonist amphetamine has been shown to induce a patchy pattern of immediate early gene (IEG) expression in the rostral striatum of both pharmacologically intact and reserpinized rats. The available data suggest that stimulation of D(2) dopamine receptors may play a role in the patterning of amphetamine-induced IEG expression, but direct evidence is lacking. In the current study of reserpinized animals, we found that pretreatment with the selective D(2) dopamine antagonist raclopride did not block the induction of the IEGs Fos and Arc by amphetamine, but greatly reduced the "patchiness" of the induced expression. Raclopride did not induce Fos or Arc expression by itself under the conditions studied here. These findings suggest that although stimulation of D(2) receptors is not necessary for amphetamine to induce IEG expression in reserpinized animals, these receptors do play a critical role in the spatial patterning of the resulting response.
Wirtshafter
David
D
Laboratory of Integrative Neuroscience, Department of Psychology, M/C 285, University of Illinois at Chicago, 100 W. Harrison Street, Chicago, IL 60607-7137, USA. davew@uic.edu
Sheppard
Amy C
AC
D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D018492:Dopamine Antagonists / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D017781:Genes, Immediate-Early / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D020891:Raclopride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000502:physiology*; D012110:Reserpine / Q000494:pharmacology*
D018759:Adrenergic Uptake Inhibitors; D003598:Cytoskeletal Proteins; D018492:Dopamine Antagonists; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D017448:Receptors, Dopamine D2; C092069:activity regulated cytoskeletal-associated protein; D020891:Raclopride; D012110:Reserpine; D000661:Amphetamine