Allergen-specific IgG and IgA in serum and bronchoalveolar lavage fluid in a model of experimental feline asthma.
Allergic asthma, a Th2 cell driven response to inhaled allergens, has classically been thought of as predominantly mediated by IgE antibodies. To investigate the role of other immunoglobulin classes (e.g., IgG and IgA) in the immunopathogenesis of allergic asthma, levels of these allergen-specific immunoglobulins were measured in serum and mucosal fluids. Bermuda grass allergen (BGA)-specific IgG and IgA ELISAs in serum and bronchoalveolar lavage fluid (BALF) were developed and optimized in an experimental model of BGA-induced feline asthma. Levels of BGA-specific IgG and IgA significantly increased over time in serum and BALF after allergen sensitization. Additionally, these elevated levels of BGA-specific IgG and IgA were seen in conjunction with the development of an asthmatic phenotype indicated by positive intradermal skin tests, enhanced airways hyperreactivity, and increased eosinophil percentages in the BALF.
Norris
C R
CR
Departments of Pathology, Microbiology and Immunology, One Shields Avenue, School of Veterinary Medicine, University of California, Davis, CA 95616-8734, USA. cnorris@ucdavis.edu
Byerly
J R
JR
Decile
K C
KC
Berghaus
R D
RD
Walby
W F
WF
Schelegle
E S
ES
Hyde
D M
DM
Gershwin
L J
LJ
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000662:veterinary*; D016535:Bronchial Hyperreactivity; D001985:Bronchial Provocation Tests / Q000662:veterinary; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D031744:Cynodon / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D016210:Methacholine Chloride / Q000276:immunology; D012129:Respiratory Function Tests / Q000662:veterinary
D000485:Allergens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D016210:Methacholine Chloride