[Effect of endogenous enzymes on composition of oligonucleotides isolated from gonads of hydrobionts].
Preparations of low-molecular-weight DNA were obtained from gonads of various hydrobiont species by alcohol precipitation technique. An enzymatic hydrolysis-based method for producing soluble low-molecular-weight DNA was developed. All the nucleic acid components of the preparations obtained were separated by electrophoresis, and their molecular weights were determined. It was demonstrated that high activities of endogenous enzymes (nucleases and proteases) in milts of hydrobionts correlated with the degree of hydrolysis of their nucleic acids.
Pozdniakova
Iu M
IuM
Pacific Research Fisheries Center (TINRO Center), Vladivostok, 690950, Russia.
Pivnenko
T N
TN
Kas'ianenko
Iu I
IuI
D000438:Alcohols; D000818:Animals; D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004586:Electrophoresis; D010450:Endopeptidases / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D006066:Gonads / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008970:Molecular Weight; D008974:Mollusca / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010194:Pancreatin / Q000378:metabolism
D000438:Alcohols; D009841:Oligonucleotides; D010194:Pancreatin; D004247:DNA; D003851:Deoxyribonucleases; D010450:Endopeptidases