The expression of melanogenic proteins in Korean skin after ultraviolet irradiation.
For proper melanin production, several specific enzymes such as tyrosinase, tyrosinase-related protein 1 (TRP-1) and dopachrome tautomerase are required. Their expressions are increased after exposure to UVB. However, it is not known how long tyrosinase and TRP-1 activities continue after UV irradiation in vivo. The purpose of this study is to measure the changes in expressions of tyrosinase, TRP1, and MITF after exposure to UV on skin in a Korean population. We established an immunohistochemical staining protocol for specimens which were obtained from UV-irradiated skin in five healthy Korean males on the 2nd, 5th, 7th, 28th, and 56th days after UV irradiation. Tyrosinase, TRP-1, and MITF expressions increased until 7 days after UV irradiation and then dropped to the basal constitutive level 4 and 8 weeks later. Interestingly, tyrosinase increased prior to TRP-1. This study reveals the time-sequence of melanin-synthesized enzymes and provides important information for the clinical evaluation of the effectiveness of whitening agents.
Kim
Tae Jin
TJ
Department of Dermatology, College of Medicine, Soonchunhyang University, Seoul, Korea.
Cho
Moon Keun
MK
Lee
Jong Suk
JS
Whang
Kyu Uang
KU
Jin
So Young
SY
Hoshino
Taku
T
D000328:Adult; D044466:Asian People / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007370:Interferon Type I / Q000378:metabolism / Q000528:radiation effects; D007723:Korea; D008297:Male; D008543:Melanins / Q000096:biosynthesis*; D051739:Microphthalmia-Associated Transcription Factor; D014442:Monophenol Monooxygenase / Q000378:metabolism / Q000528:radiation effects; D011257:Pregnancy Proteins / Q000378:metabolism / Q000528:radiation effects; D011506:Proteins / Q000378:metabolism* / Q000528:radiation effects; D012016:Reference Values; D012867:Skin / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D012880:Skin Pigmentation / Q000235:genetics / Q000528:radiation effects*; D014157:Transcription Factors / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D007370:Interferon Type I; C497234:MITF protein, human; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D011257:Pregnancy Proteins; D011506:Proteins; D014157:Transcription Factors; C020947:interferon tau; D014442:Monophenol Monooxygenase