[Water-soluble galactomannan from the seeds of Lotus corniculatus L.: structure and properties].
Galactomannan, a water-soluble heteropolysaccharide, was isolated from the seed of Far-Eastern population of ground honeysuckle Lotus corniculatus L. (yield, 1.65%). Analysis of this galactomannan showed that is consists of D-mannose and D-galactose residues (molar ratio, 1.22:1). Its aqueous solutions were characterized by specific rotation [alpha]D = +84.10 and characteristic viscosity [eta] = 559 ml/g. Analysis of this heteropolysaccharide using chemical and enzymatic procedures, as well as IR- and 13C-NMR spectroscopy, showed that its main chain comprises 1,4-beta-D-mannopyranose residues, 95.5% of which are substituted at C-6 with single residues of alpha-D-galactopyranose.
Egorov
A V
AV
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Mestechkina
N M
NM
Plennik
R Ia
RIa
Shcherbukhin
V D
VD
D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D000070116:Lotus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D014783:Viscosity
D008351:Mannans; C012990:galactomannan; D008358:Mannose; D005690:Galactose