34-BetaE12 expression in benign and premalignant squamous lesions of skin: relation to cell proliferation (Ki-67).
The antibody 34-betaE12 stains selectively the keratins of basal cells. The aim of this study is to investigate the staining pattern of 34-betaE12 in borderline (keratoacanthomas and solar keratosis), and benign lesions (seborrheic keratoses). The proliferation index Ki-67 staining was also evaluated in these and also in malignant (basal and squamous cell carcinomas) cases. The staining pattern where the 34-betaE12 positive cells found in the basal, suprabasal epidermal layers was called "focal"; and the staining in all layers including upper spinous layer was called "diffuse". Mean proliferation index and the distribution pattern of Ki-67 immunohistochemical expression were assessed. Basal and suprabasal expression of 34-betaE12 significantly predominated in the normal parts of the epidermis, in eight out of 11 seborrheic keratoses (%73), one out of the 19 keratoacanthomas (%5), two out of 11 solar keratosis (18%). Statistical analysis revealed significant differences between mean level of Ki-67 expression of malignant (squamous cell carcinoma, basal cell carcinoma), benign (seborrheic keratoses) and premalignant (solar keratoses, keratoacanthomas) lesions (p<0.01). The distribution of staining pattern for Ki-67 paralleled to the staining pattern of 34-betaE12. Basal cell status assessment completed by 34-betaE12 may resolve some, but not all of the problems in terms of determining the presence of dysplasia.
University of Mersin School of Medicine, Department of Pathology, Mersin, Turkey. dusmezduygu@hotmail.com
D
D
Düşmez Apa
O
O
Aydin
A
A
Polat
L
L
Cinel
Y
Y
Polat
R
R
Egilmez
D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism*; D007636:Keratoacanthoma / Q000378:metabolism / Q000473:pathology; D007642:Keratosis / Q000378:metabolism / Q000473:pathology; D017492:Keratosis, Seborrheic / Q000378:metabolism / Q000473:pathology; D019394:Ki-67 Antigen / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology*; D012867:Skin / Q000378:metabolism* / Q000473:pathology*
C121278:CK-34 beta E12; D019394:Ki-67 Antigen; D007633:Keratins