[Standardization of glycosylated hemoglobin in the context of the DAI study].
The results obtained from 85 antidiabetic centers enrolled in the DAI study are presented with regard to the external quality assessment scheme for glycohemoglobin. The materials have been prepared by a laboratory of the network of reference laboratories of the International Federation of Clinical Chemistry (IFCC). To each control a Diabetes Control and Complications Trial (DCCT) traceable target value was assigned. The High-Performance Liquid Chromatography (HPLC) methods for glycohemoglobin are used in 75% of the centers, the immunochemical techniques in 21% and less than 5% is using affinity chromatography based methods. The data collected from the laboratories who completed the set of measurements show that 64% of the centers are well aligned to the DCCT system. The reproducibility of the methods varied between 3.7 and 5.8% (as CV, %) and has to be improved.
Mosca
Andrea
A
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi, Milano. andrea.mosca@unimi.it
Paleari
Renata
R
D002138:Calibration; D002846:Chromatography, Affinity / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D006442:Glycated Hemoglobin / Q000032:analysis / Q000592:standards*; D006452:Hemoglobinometry / Q000379:methods / Q000592:standards*; D006801:Humans; D007120:Immunochemistry / Q000592:standards; D007558:Italy; D011785:Quality Assurance, Health Care; D012015:Reference Standards; D015203:Reproducibility of Results
D006442:Glycated Hemoglobin