Enhanced sensitivity and spatial resolution for in vivo imaging with low-level light-emitting probes by use of biocompatible chemical agents.
We describe a technique that uses biocompatible chemical agents to enhance both the sensitivity and the resolution of in vivo imaging with low-level light-emitting probes. We demonstrate experimentally, with chemiluminescence (CL) imaging in vitro as an example, that the detected intensity of CL from treated 3-mm-thick skin tissue is approximately fivefold stronger than that from untreated skin. The spatial resolution correspondingly increases approximately threefold.
Biophotonics Laboratory, Institute of BioScience and Technology, Cranfield University at Silsoe, Silsoe, Bedfordshire MK45 4DT, UK.
Yonghong
Y
He
Ruikang K
RK
Wang
Da
D
Xing
D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000494:pharmacology*; D003952:Diagnostic Imaging; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology; D008027:Light; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009995:Osmosis; D016877:Oxidants / Q000494:pharmacology; D012867:Skin / Q000033:anatomy & histology* / Q000378:metabolism; D013552:Swine
D001672:Biocompatible Materials; D007202:Indicators and Reagents; D016877:Oxidants; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase