Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa.
Most clinical isolates identified as Pseudomonas aeruginosa grow on long-chain n-alkanes, while environmental P. aeruginosa isolates often grow on medium- as well as long-chain n-alkanes. Heterologous expression showed that the two alkane hydroxylase homologs of P. aeruginosa PAO1 (AlkB1 and AlkB2) oxidize C(12)-C(16)n-alkanes, while two rubredoxin (RubA1 and RubA2) and a rubredoxin reductase (RubB) homologs can replace their P. putida GPo1 counterparts in n-octane oxidation. The two long-chain alkane hydroxylase genes are present in all environmental and clinical isolates of P. aeruginosa strains tested in this study.
Smits
Theo H M
TH
ETH Hönggerberg, Institute of Biotechnology, Zürich, CH-8093, Switzerland; Current address: EPFL, ENAC/ISTE/LBE, Lausanne, CH-1015, Switzerland.
Witholt
Bernard
B
van Beilen
Jan B
JB
D000473:Alkanes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D012416:Rubredoxins / Q000235:genetics / Q000378:metabolism
D000473:Alkanes; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012416:Rubredoxins; D042926:Cytochrome P-450 CYP4A; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases