A high-performance liquid chromatographic-tandem mass spectrometric method for the determination of cethromycin (ABT-773) in human plasma, bronchoalveolar lavage fluid, and alveolar cells.
A method is developed for the specific and sensitive determination of cethromycin concentrations in plasma, bronchoalveolar lavage (BAL), and alveolar cells (AC), using a high-performance liquid chromatographic-tandem mass spectrometry (MS) method. The mobile phase consists of 50% acetonitrile-0.05% acetic acid-5mM ammonium acetate; the column used is a C(8) reversed-phase stationary phase. The preparation of samples requires a solvent extraction step. The retention times for cethromycin and the internal standard are approximately 2.0 and 2.7 min, respectively, with a total run time of 3.5 min. Detection is carried out using electrospray MS in a multiple reaction monitor mode. The detection limits for cethromycin are 1 ng/mL for plasma and 0.2 ng/mL for BAL supernatants and AC suspensions. The assay has excellent performance characteristics and has been used to support a study of the intrapulmonary pharmacokinetics of cethromycin in human subjects.
Department of Biopharmaceutical Sciences, University of California, San Francisco, San Francisco, CA 94117, USA.
Qiulei
Q
Ren
John E
JE
Conte
Elisabeth
E
Zurlinden
Emil T
ET
Lin
D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood; D006801:Humans; D048628:Ketolides; D011650:Pulmonary Alveoli / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D048628:Ketolides; D004917:Erythromycin; C405499:cethromycin