In vivo expression of recombinant pregnancy-specific glycoprotein 1a induces alternative activation of monocytes and enhances Th2-type immune response.
It has been proposed that pregnancy-specific factors could be responsible for shift the balance of cytokine profiles during maternal immune response from Th1-type reactivity into a "less-damaging" Th2-type reactivity. In the present work, we investigated the in vivo function of human pregnancy-specific glycoprotein (PSG)1a, the major variant of PSG polypeptides released into the circulation during pregnancy, on the modulation of the innate and adaptive immune response. For this, BALB/c mice were injected with a vaccinia virus-based vector harboring the human PSG1a cDNA (Vac-PSG1a) 4 days before immunization with ovalbumin (OVA) in complete Freund's adjuvant, and the early specific T cell response against OVA was evaluated 8 days post-immunization. We also studied the activation status of spleen and peritoneal monocytes/macrophages (Mo) populations from Vac-PSG1a-treated mice, and explored whether PSG1a-targeted Mo could affect the Th-type commitment by investigating their impact on the differentiation of naive T cells. Our data show that the treatment with Vac-PSG1a is able to induce a state of alternative activation on Mo. Furthermore, the generation of the immune response in the context of these alternatively activated antigen-presenting cells may shift T cell differentiation to Th2-type immunity which is more compatible with a successful pregnancy.
Motrán
Claudia C
CC
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. cmotran@bioclin.fcq.unc.edu.ar
Diaz
Fernando López
FL
Montes
Carolina L
CL
Bocco
José Luis
JL
Gruppi
Adriana
A
D000818:Animals; D002455:Cell Division / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000235:genetics*; D013154:Spleen / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; D006023:Glycoproteins; D011257:Pregnancy Proteins; D016753:Interleukin-10