Antioxidant status of dog aqueous humor after extracapsular lens extraction.
We determined the antioxidant status of the aqueous humor after extracapsular lens extraction in 14 mongrel dogs weighing about 10 kg. The animals were examined by slit lamp biomicroscopy, applanation tonometry and indirect ophthalmoscopy. One eye was submitted to conventional extracapsular lens extraction and the other was used as control. Samples of aqueous humor were obtained by anterior chamber paracentesis before and at days 1, 2, 3, 7 and 15 after surgery. Total antioxidant status was determined as the capacity of aqueous humor to inhibit free radical generation by 2,2-azobis(2-amidopropane) chlorine. Ascorbic acid concentration was measured by HPLC with UV detection. Protein content was determined with the biuret reagent. Statistical analysis was performed by ANOVA followed by the Tukey-Kramer test. Protein concentration increased from 0.61 to 22 mg/ml 24 h after surgery. These levels were maintained and returned to normal at day 7. Total antioxidant capacity was reduced from 50 to about 30 min until day 3 and at day 7 it was equal to control. Ascorbic acid levels were reduced from 252 to about 110 microM and then returned to control values at day 15. Considering the importance of ascorbic acid concentration in aqueous humor for the maintenance of the antioxidant status of the anterior segment of the eye, the decrease of antioxidant defenses suggests that the surgical procedures promote an oxidative stress condition in the eye.
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil. pauloeye@usp.br
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Barros
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Padovani
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Silva
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Queiroz
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Barros
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001082:Aqueous Humor / Q000737:chemistry / Q000378:metabolism*; D001205:Ascorbic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005136:Eye Proteins / Q000032:analysis; D007908:Lens, Crystalline / Q000601:surgery*; D018384:Oxidative Stress; D013997:Time Factors
D000975:Antioxidants; D005136:Eye Proteins; D001205:Ascorbic Acid