[Silica nanoparticles modified as carriers for gene transfection].
The silica nanoparticles are modified by sodium iodine or sodium chloride with different concentration. Their ability to bind and protect plasmid DNA was demonstrated by agarose gel electrophoresis. The EGFP-N1 plasmid was transfected into HT1080 by combing silica nanoparticle. Eletromicroscope examine revealed that nanoparticle-DNA could enter into cells, these silica nanoparticles protect DNA against nuclease digestion and the GFP has an effective expression. In conclusion, these nanoparticles might be used as DNA carriers for gene transfection.
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China. zhigangxue@hotmail.com
Zhi-Gang
ZG
Xue
Duo
D
Zheng
Jian-Ming
JM
Ruan
Qian
Q
Pan
Di-Cheng
DC
Zhao
Xiao-Ping
XP
Liu
Yu-Xiang
YX
Chen
Jia-Hui
JH
Xia
Kun
K
Xia
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008863:Microspheres; D012822:Silicon Dioxide / Q000008:administration & dosage* / Q000633:toxicity; D012965:Sodium Chloride / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
D012965:Sodium Chloride; D012822:Silicon Dioxide