Measurement of the levels of polymerized actin (F-actin) in chemokine-stimulated lymphocytes and GFP-coupled cDNA transfected lymphoid cells by flow cytometry.
Immunology Service, Hospital Universitario de la Princesa, UAM, Madrid, Spain.
Miguel
M
Vicente-Manzanares
Mariano
M
Vitón
Francisco
F
Sánchez-Madrid
D000199:Actins / Q000032:analysis* / Q000378:metabolism; D018925:Chemokines / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D049452:Green Fluorescent Proteins; D006801:Humans; D066298:In Vitro Techniques; D019169:Jurkat Cells; D008164:Luminescent Proteins / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
D000199:Actins; D018925:Chemokines; D018076:DNA, Complementary; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins