Rapid fibroblast adhesion to 27nm high polymer demixed nano-topography.
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.
Dalby
M J
MJ
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, G12 8QQ, UK. m.dalby@bio.gla.ac.uk
Giannaras
D
D
Riehle
M O
MO
Gadegaard
N
N
Affrossman
S
S
Curtis
A S G
AS
D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D013343:Styrenes / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D020744:rac GTP-Binding Proteins / Q000378:metabolism
D001672:Biocompatible Materials; D045424:Complex Mixtures; D008567:Membranes, Artificial; D011108:Polymers; D011137:Polystyrenes; D013343:Styrenes; C018238:beta-bromostyrene; D020744:rac GTP-Binding Proteins