[Interaction of human S26 ribosomal protein with fragments of mRNA and pre-mRNA for this protein in Hela nuclear cell extracts].
As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, recombinant rpS26 binds to the first intron of the rpS26 pre-mRNA (apparent association constant (Ka) approximately 5.0 x 10(7) M-1) and, to a lesser extent, to the rpS26 mRNA (Ka approximately 2.0 x 10(7) M-1). The binding was specific, since human rpS19 had an order of magnitude lower Ka with the first intron and did not bind with the rpS26 mRNA. Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA. In either case, RNA binding substantially increased in the presence of recombinant rpS26. Along with other (48 K, 59 K) nuclear proteins, rpS26 was assumed to form complexes, the functional role of which is storage of pre-mRNAs inactive in splicing.
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
A V
AV
Ivanov
A A
AA
Malygin
G G
GG
Karpova
D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D017931:DNA Primers; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay; D012322:RNA Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D017931:DNA Primers; D012322:RNA Precursors; D012333:RNA, Messenger; C489203:RPS26 protein, human; D012269:Ribosomal Proteins