Effective bead preparation of coimmobilized methanogenic and methanotrophic bacteria for tetrachloroethene degradation.
Three types of coimmobilized methanogenic and methanotrophic bacterial beads--Ca-alginate, Ba-alginate, and Ca-alginate chitosan--were used for tetrachloroethene (PCE) degradation. For the purpose of effective preparation of coimmobilized bacterial beads, the diameter and broken-loading of beads were measured. The activity tests to find the optimal bacteria concentration in the bead were performed. It was found that Ba-alginate beads had superiority in bacterial growth and the degree of strength of beads from the diameter and broken-loading tests. Also, it was shown that it is most effective to add 200 mL of methanogens into 500 mL of 2% alginate solution and 20 mL of methanotrophs into 500 mL to 2% alginate solution. When methanogens and methanotrophs were applied with the Ba-alginate bead in the actual dechlorination of PCE, the biological PCE dechlorination rate was 92%, and there was highly effective degradation of PCE based on the coimmobilized bead. Additionally, relation to the diameter (X) and broken-loading (Y) of the Ba-alginate bead was derived following equation, Y = 438.02 exp(-1.4815 X).
School of Civil & Environmental Engineering, College of Engineering, Yonsei University, Seoul (120-749), Korea. sludge@dreamwiz.com
Yoo
Y
Sung-In
Choung
C
Youn-Kyoo
Lee
L
Byung-Chan
D001419:Bacteria / Q000378:metabolism*; D001420:Bacteria, Aerobic / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000378:metabolism; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D010316:Particle Size; D013750:Tetrachloroethylene / Q000378:metabolism*; D014871:Water Microbiology
D004785:Environmental Pollutants; D008697:Methane; D013750:Tetrachloroethylene