[Effect of osmolar concentration of culture media on exocytosis in isolated presynaptic nerve endings of rat brain ].
The effect of hypotonic and hypertonic shock on exocytosis in rat brain synaptosomes was studied using the fluorescent dye acridine orange. It was shown that an increase in medium osmolarity leads to calcium-independent exocytosis. The response of the probe was directly proportional to the amount of osmolithes added. A decrease in medium osmolarity to 230 mOsm led to an increase of acridine orange fluorescence, which is comparable with exocytosis occurring by the action of 15 mM KCl. This effect was independent of calcium concentration. It is assumed that, under hypotonic shock, part of neurotransmitters are released from the vesicular pool.
Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
T V
TV
Vasim
S V
SV
Fedorovich
S V
SV
Konev
D000165:Acridine Orange; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005089:Exocytosis; D005456:Fluorescent Dyes; D009474:Neurons / Q000378:metabolism*; D009994:Osmolar Concentration; D017729:Presynaptic Terminals / Q000378:metabolism*; D051381:Rats
D005456:Fluorescent Dyes; D000165:Acridine Orange