Ion trap tandem mass spectrometric determination of F2-isoprostanes.
A new tandem mass spectrometry (MS/MS) method based on the use of an ion trap mass spectrometer for the identification and quantitation of F(2)-isoprostanes has been developed. It consists of two solid-phase extractions and two derivation steps followed by gas chromatography/negative ion chemical ionization tandem mass spectrometry (GC/NICI-MS/MS) analysis. This method is highly selective and sensitive and it has been successfully applied to biological samples.
Department of Pathophysiology, Experimental Medicine and Public Health, University of Siena, Via Aldo Moro, 53100 Siena, Italy.
Cinzia
C
Signorini
Mario
M
Comporti
Gianluca
G
Giorgi
D000818:Animals; D002138:Calibration; D028441:F2-Isoprostanes / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D051381:Rats; D012680:Sensitivity and Specificity
D028441:F2-Isoprostanes; D007477:Ions