A novel extracellular protein of Streptomyces peucetius binds to daunorubicin but does not inhibit the bioactivity of the antibiotic.
Extracellular proteins of Streptomyces peucetius that bind to a red pigment were identified during the course of isolation of mutants defective in daunorubicin production. Two pigment-protein complexes were partially purified and this complex inhibited the growth of Bacillus subtilis. Routine solvent extraction could not separate the pigment from purified pigment-protein complex. Treatment with 2% SDS at 100 degrees C followed by solvent extraction also failed to separate the protein from the pigment. However, the pigment could be separated from the purified pigment-protein complex by heating it in 0.1M HCl at 100 degrees C followed by solvent extraction. The pigment extracted from the complex was analyzed by HPLC and found to be daunorubicin.
Prasad
Ranjan
R
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India.
Sasikala
V
V
Vetrivel
K S
KS
Dharmalingam
K
K
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003630:Daunorubicin / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003630:Daunorubicin