Localization of projectin in locust flight muscle.
Projectin is a giant filamentous protein of arthropod striated muscle. By using immunofluorescence microscopy, projectin was shown to span between the I band and the A band in locust (Locusta migratoria) flight muscle sarcomeres. The N- and C-terminal regions of projectin molecules were localized in the I band and A band, respectively. This observation explains the controversial reports of previous studies that projectin is localized either in the I band or in the A band of locust flight muscle sarcomeres. It is also observed that the N-terminal region of projectin is located in the I band of locust leg muscle sarcomeres.
Department of Biology, Faculty of Science, Chiba University, Chiba 263-8522, Japan.
Jinen
J
Shimamura
Koscak
K
Maruyama
Sumiko
S
Kimura
D000818:Animals; D005426:Flight, Animal / Q000502:physiology*; D007313:Insecta; D008856:Microscopy, Fluorescence; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000166:cytology; D012518:Sarcomeres / Q000737:chemistry*
D009124:Muscle Proteins; C078033:projectin