Serum levels of chemokines correlate with disease activity in patients with retinal vasculitis.
Retinal vasculitis (RV) is characterised pathologically by migration of leucocytes across the blood-retinal barrier leading to oedema and photoreceptor cell dysfunction. Chemokines are a family of small molecules involved in leucocyte migration. In this study, levels of chemokines were measured in serum from patients with RV and correlated with disease activity and drug treatment. Serum samples (n= 100; 25 active, 75 inactive) were obtained from 50 patients with RV, and levels of the chemokines MIP-1alpha, macrophage inflammatory protein-1beta (MIP-1beta) and monocyte chemoattractant protein-1 (MCP-1) were measured by ELISA. For longitudinal analysis levels of the same chemokines were measured in six consecutive serum samples from 10 of the above patients. Chemokine levels were correlated with disease activity and current drug treatment for each sample. Sera from 20 healthy individuals were used as control samples. Serum levels of MIP-1beta were significantly raised in patients with RV, whether active or not, compared to healthy controls (P= 0.04). Levels of MIP-1beta and MCP-1 correlated with disease activity in some patients and with prednisolone levels in patients on this treatment alone. MIP-1alpha levels were not detectable in all samples but were present in significantly more samples from patients with active or inactive RV compared with healthy controls. Serum levels of the chemokines MIP-1beta and MIP-1alpha, but not MCP-1 were raised in patients with retinal vasculitis. Longitudinal analysis suggested that MIP-1beta and MCP-1 levels were controlled by drug treatment, particularly prednisolone. These data demonstrate that chemokines are involved in the pathogenesis of RV and may act as novel therapeutic targets.
Wallace
Graham R
GR
Department of Ophthalmology, GKT, St. Thomas' Hospital, London SE1 7EH, UK. g.r.wallace@bham.ac.uk
Farmer
Ian
I
Church
Alison
A
Graham
Elizabeth M
EM
Stanford
Miles R
MR
D018932:Chemokine CCL2 / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use; D012008:Recurrence; D031300:Retinal Vasculitis / Q000097:blood* / Q000473:pathology
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D011239:Prednisolone