Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface.
With the help of a microfabrication process and surface modification technology, a method of fabricating protein patterned chips was developed which can be utilized as a powerful tool for performing bioassays in a high-throughput manner. A digital micromirror array (MMA) system was used as a virtual photomask, so that a maskless photolithography process was able to be used to build patterned biomolecules on a chip by selective illumination onto the chip surface. We utilized the nitroveratryloxycarbonyl (NVOC) group as a photolabile protecting group for protein patterning. The NVOC-protected surface was selectively irradiated by a UV illuminator using an MMA. After removing the NVOC group, biotin was coupled to the NVOC-cleaved site, onto which a buffered streptavidin solution was eluted. At this point, we could obtain a streptavidin-patterned surface and observe the effect of the polymer-grafted surface in reducing nonspecific binding.
Shin
Dong-Sik
DS
School of Chemical Engineering, Seoul National University, Kwanak-Gu, Seoul 151-742, South Korea.
Lee
Kook-Nyung
KN
Jang
Ki-Hoon
KH
Kim
Jae-Kwon
JK
Chung
Woo-Jae
WJ
Kim
Yong-Kweon
YK
Lee
Yoon-Sik
YS
D000327:Adsorption; D001710:Biotin / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D005898:Glass; D010781:Photography / Q000379:methods; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D011108:Polymers; D014867:Water; D001710:Biotin; D019809:Streptavidin