Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics.
The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH(2))(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microscopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.
Culha
Mustafa
M
Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Bethel Valley Road, MS-6101 PO Box 2008, Oak Ridge, Tennessee 37831-6101, USA.
Stokes
David
D
Allain
Leonardo R
LR
Vo-Dinh
Tuan
T
D019313:BRCA1 Protein / Q000235:genetics*; D015342:DNA Probes / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D000441:Hexanols; D006801:Humans; D015394:Molecular Structure; D012235:Rhodamines; D012680:Sensitivity and Specificity; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods*
D019313:BRCA1 Protein; D015342:DNA Probes; D004277:DNA, Single-Stranded; D000441:Hexanols; D012235:Rhodamines; D012834:Silver; C029773:rhodamine B