[Study on the genome structure of human telomeric repeat binding factor 1 and its pseudogenes].
To determine the genome structure of human telomeric repeat binding factor 1 (TERF1) and its pseudogenes.



Sequences were obtained from GenBank and analyzed using the BLAST program and other relevant biology program (Sequencher, DNA Strider and Autoassembler, etc) to determine the genome and pseudogenome structure of TERF1. PCR and sequencing were performed to verify the results.



TERF1 gene which mapped to 8q13 was divided into 10 exons. It had four processed pseudogenes located on chromosome 13, 18, 21 and X respectively (Psi TERF1-13 Psi TERF1-18 Psi TERF1-21 and Psi TERF1-X ). They were entire intronless TERF1 genes which lacked some exons. Three homologous fragments of at least 60 kb on the flanking region of Psi TERF1-13, Psi TERF1-18 and Psi TERF1-21, respectively were noted.



TERF1 gene has 10 exons. It has four processed pseudogenes which are located on chromosome 13, 18, 21, and X, respectively. Large homologous fragments that belong to the recently duplicated segments are transchromosomal duplications.
Sun
Jie
J
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Huang
He
H
Song
Huai-dong
HD
Wu
Xin-yan
XY
D002874:Chromosome Mapping; D040342:Genetic Structures; D006801:Humans; D011544:Pseudogenes; D035321:Telomeric Repeat Binding Protein 1 / Q000235:genetics*
D035321:Telomeric Repeat Binding Protein 1