title: Quinides of roasted coffee enhance insulin action in conscious rats.
abstract: Consumption of large amounts of coffee has been shown to decrease the incidence of type 2 diabetes. However, the specific compounds and mechanisms responsible for this effect are not known. The aim of this study was to determine the effects of a decaffeinated coffee extract and a synthetic quinide, representative of those found in roasted coffee, 3,4-diferuloyl-1,5-quinolactone, on insulin-stimulated glucose disposal and muscle glucose uptake. Experiments were performed on conscious rats during hyperinsulinemic, euglycemic clamps receiving gastric infusions of saline, a decaffeinated coffee extract (DECAF) (220 mg/kg), or 3,4-diferuloyl-1,5-quinide (DIFEQ) (110 mg/kg). Following treatment, rats received an intravenous bolus of deoxy-[2-3H] glucose to assess muscle glucose uptake (Rg, micromol x 100 g(-1) x min(-1)). Glucose infusions [mg/(kg x min)] required to maintain euglycemia during the tracer period were higher with DIFEQ (14.6 +/- 0.7) than with saline (10.8 +/- 0.7) and DECAF (11.5 +/- 1.1). Despite increased glucose requirements, Rg in skeletal (soleus, gastrocnemius, superficial vastus lateralis) and cardiac muscle were unchanged. DECAF or DIFEQ did not affect heart rate, blood pressure, plasma nonesterified fatty acids or liver aminotransferase activity. These results demonstrate that DIFEQ increases whole-body glucose disposal independently of skeletal muscle Rg.
authors: Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA. jane.shearer@vanderbilt.edu
authors: Jane
authors: J
authors: Shearer
authors: Adriana
authors: A
authors: Farah
authors: Tomas
authors: T
authors: de Paulis
authors: Deanna P
authors: DP
authors: Bracy
authors: R Richard
authors: RR
authors: Pencek
authors: Terry E
authors: TE
authors: Graham
authors: David H
authors: DH
authors: Wasserman
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D003069:Coffee / Q000737:chemistry*; D003373:Coumaric Acids / Q000494:pharmacology*; D003847:Deoxyglucose / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D015309:Glucose Clamp Technique; D006339:Heart Rate / Q000187:drug effects; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium
chemical_list: C465242:3,4-diferuloyl-1,5-quinolactone; D001786:Blood Glucose; D003069:Coffee; D003373:Coumaric Acids; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007783:Lactones; D014316:Tritium; D003847:Deoxyglucose