In vivo calibration of the subcutaneous amperometric glucose sensors using a non-enzyme electrode.
A new two-point calibration method for the subcutaneous amperometric continuous glucose sensor is reported. The proposed method is based on direct measurement of the background current (I(o)) using a non-enzyme electrode. For in vivo test, three electrodes were implanted in rabbits. Two of the three were identical needle-type enzyme electrodes with perfluorinated polymer outer layers (Pt/enzyme layer/Kel-F/PTFE/Kel-F/Nafion) that were placed in subcutaneous tissue and in a vessel (ear artery), respectively. And one non-enzyme electrode with exactly the same membrane composition as those of other two was in the subcutaneous layer to measure the background current. Implantation in the subcutaneous layer generated many crevices on the protecting layers of the electrodes. The signals from enzyme electrodes were effectively corrected by the measured background current from the non-enzyme electrode. In addition, a telemetric monitoring system was developed and evaluated for in vivo continuous glucose monitoring in order to alleviate the problems of motion artifact.
Jeong
Ran-A
RA
Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 28 Yongon Dong, Chongno Gu, Seoul 110-744, South Korea.
Hwang
Jae Youn
JY
Joo
Segyeong
S
Chung
Taek Dong
TD
Park
Sejin
S
Kang
Sun Kil
SK
Lee
Won-Yong
WY
Kim
Hee Chan
HC
D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D005240:Feasibility Studies; D008297:Male; D018670:Monitoring, Ambulatory / Q000295:instrumentation* / Q000379:methods; D019736:Prostheses and Implants; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001786:Blood Glucose