title: Mercury contamination of rat amylin mimics vasoactivity and cytotoxic effects.
abstract: Rat amylin differs from human amylin (hIAPP) in that it lacks a fibril-forming capacity. As a consequence, toxic effects have been reported for human but not for rat amylin. This report demonstrates how a mercury contamination of commercial rat amylin imitates peptide-related vasoactive and cytotoxic effects on preparations of isolated blood vessels. The source of mercury contamination was believed related to the peptide synthesis. Thiol groups of cysteine-containing peptides are often protected by acetamidomethyl (Acm) which is cleaved by mercuric acetate.
authors: Department of Internal Medicine, Philipps University of Marburg, Baldinger Strasse, 35033, Marburg, Germany. Heiko.Golpon@medizin.uni-magdeburg.de
authors: Heiko A
authors: HA
authors: Golpon
authors: Annette
authors: A
authors: Püchner
authors: Lothar
authors: L
authors: Schmidt
authors: Hartmut
authors: H
authors: Jungclas
authors: Peter
authors: P
authors: Barth
authors: Tobias
authors: T
authors: Welte
authors: Peter V
authors: PV
authors: Wichert
authors: Christian O
authors: CO
authors: Feddersen
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000682:Amyloid / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D002142:Californium / Q000378:metabolism; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008297:Male; D013058:Mass Spectrometry; D008628:Mercury / Q000378:metabolism* / Q000633:toxicity; D011651:Pulmonary Artery / Q000187:drug effects*; D051381:Rats
chemical_list: D000682:Amyloid; D000897:Anti-Ulcer Agents; D058228:Islet Amyloid Polypeptide; D002142:Californium; D008628:Mercury; D000109:Acetylcholine