title: Development of cancer vaccines using autologous and recombinant high molecular weight stress proteins.
abstract: High molecular weight heat shock proteins (HSPs), hsp110 and grp170, derived from cancer cells have been previously shown to elicit tumor-specific immunity. This phenomenon is attributed to the antigenic peptides associated with the HSPs. Based on the unique chaperoning properties of these HSPs, a new vaccination strategy has been recently developed to elicit antigen-specific antitumor immunity. This approach utilizes tumor-associated antigens naturally complexed to these highly efficient molecular chaperones under heat shock conditions. This chapter focuses on the methodologies of these two vaccine strategies: I. purification of hsp110 and grp170 from tumor tissue or cell lines; II. generation and characterization of in vitro HSP-antigen complexes by heat shock using recombinant HSPs derived from a baculovirus protein expression system.
authors: Department of Molecular and Cellular Biophysics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. Xiang-yang.Wang@roswellpark.org
authors: Xiang-Yang
authors: XY
authors: Wang
authors: Masoud H
authors: MH
authors: Manjili
authors: Juneui
authors: J
authors: Park
authors: Xing
authors: X
authors: Chen
authors: Elizabeth
authors: E
authors: Repasky
authors: John R
authors: JR
authors: Subjeck
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006031:Glycosylation; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000276:immunology* / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D009369:Neoplasms / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D017510:Protein Folding; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D019496:Cancer Vaccines; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007202:Indicators and Reagents; D011994:Recombinant Proteins