Novel employment of lactate dehydrogenase release from porcine aortic endothelial cells (PAEC) as a quantitative marker of cytotoxic activity in thermophilic Campylobacter spp. from human faecal isolates, poultry and environmental sources.
The aim of this study was to employ a novel cytotoxicity assay based on primary porcine aortic endothelial cells in combination with a lactate dehydrogenase release assay to quantitatively determine differences in cytotoxin production between Campylobacter jejuni, C. coli, C. lari and urease-positive thermophilic campylobacters (UPTC), isolated from human faeces, animals and environmental sources. Campylobacter isolates totalling 34 and comprising of C. jejuni (n = 24) C. coli (n = 5) and UPTC (n = 4) and C. lari (n = 1) were analysed. The cytotoxic response ranged from 32.15 to 64.47% and 33.08 to 59.41%, for C. jejuni from chicken and human isolates, respectively and there was no statistically significant difference (P > 0.05) in cytotoxic response between C. jejuni isolated from humans and chicken isolates (50.78% versus 50.55% cytotoxicity, respectively). However, there was a difference in response between C. jejuni and C. coli isolated from chickens (50.78% versus 33.22% cytotoxicity, respectively). The greatest cytotoxic response was obtained with the UPTC group of organisms examined (n = 4 isolates) (mean cytotoxic response = 57.11% cytotoxicity. Employment of this cytotoxin assay may help identify virulent strains in poultry that could potentially proceed to cause clinical problems for humans and thus intervention measures targeted at the reduction or elimination of such specific strains, may be sought.
Millar
B C
BC
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Northern Ireland, UK.
McCarron
M
M
Murphy
P G
PG
Moore
J E
JE
D000818:Animals; D001434:Bacteriophage Typing; D015415:Biomarkers; D002167:Campylobacter / Q000235:genetics / Q000472:pathogenicity*; D002169:Campylobacter Infections / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology; D004730:Endothelium, Vascular / Q000201:enzymology; D004781:Environmental Exposure; D005243:Feces / Q000382:microbiology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010641:Phenotype; D013045:Species Specificity; D013552:Swine
D015415:Biomarkers; D007770:L-Lactate Dehydrogenase