title: [Bifidobacterium cell wall proteins induced beta-defensin 2 mRNA expression in human intestinal epithelial cells].
abstract: OBJECTIVE
  To examine Bifidobacterium mediated induction of human defensin 2 mRNA expression in human intestinal epithelial cells and define the bioactive components of Bifidobacterium.
  
  
  METHODS
  Bifidobacterium was cultured in MRS medium in anaerobic incubator and its cell wall was isolated by sonication and centrifugation. The cell wall proteins of Bifidobacteria were extracted with 2% SDS. The level of hBD-2 mRNA in HT-29 cells was detected by reverse-transcription PCR and Northern blot analysis.
  
  
  RESULTS
  There was no detectable signal of hBD-2 mRNA in the unstimulated HT-29 cells. However, the heat-killed Bifidobacteria, cell wall of Bifidobacteria, and cell wall proteins remarkably induced hBD-2 mRNA expression in HT-29.
  
  
  CONCLUSIONS
  Bifidobacterium or its cell wall proteins can induce hBD-2 gene expression in human intestinal epithelial cells, which may play a role in the mechanisms of innate defense against pathogens in the intestine.
authors: Research Unit of Infection & Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors: Guoxing
authors: G
authors: Wang
authors: Yun
authors: Y
authors: Feng
authors: Yuhui
authors: Y
authors: Wang
authors: Ning
authors: N
authors: Huang
authors: Qi
authors: Q
authors: Wu
authors: Boyao
authors: B
authors: Wang
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D001644:Bifidobacterium / Q000166:cytology*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000166:cytology; D008565:Membrane Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C106641:DEFB4A protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D023083:beta-Defensins