Stabilizing effect of artificial stabilizers for binding activity of QCM immunosensors.
We compare stabilizing effects of artificial and natural stabilizers for maintenance of binding activity of immobilized antibody on the gold surface of quartz crystal microbalance (QCM). Binding activity between immobilizing antibody and specific antigen was measured and considered as a calculated frequency shift with QCM-immunosensor after temperature increasing and long-term storage. According to the experimental results, one stabilizer among eight samples shows the best stabilizing effect for QCM-immunosensor.
Park
Jong-Won
JW
Kurosawa
Shigeru
S
Aizawa
Hidenobu
H
Wakida
Shin-ichi
S
Yamada
Satoshi
S
Ishihara
Kazuhiko
K
D000327:Adsorption; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004355:Drug Stability; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D011791:Quartz; D013696:Temperature
D000936:Antigen-Antibody Complex; D020099:Coated Materials, Biocompatible; D011791:Quartz