title: A simple method for semi-preparative-scale production and recovery of enterocin AS-48 derived from Enterococcus faecalis subsp. liquefaciens A-48-32.
abstract: Production of enterocin AS-48 by Enterococcus faecalis A-48-32 was compared between standard and high-cell density batch fermentations. In high-cell density cultures, bacteriocin production was 2.47-fold higher, provided that the pH was controlled during the fermentation. A two-step procedure for recovery of milligram quantities of purified bacteriocin was developed, based on adsorption of the bacteriocin on Carboxymethyl Sephadex CM-25 followed by reversed-phase chromatography on a semi-preparative column. The purified bacteriocin was active on all the Gram-positive bacteria tested (for example, species of Bacillus, Paenibacillus, Staphylococcus, and Listeria). Strains E. coli U-9, E. coli CECT 102, E. coli CECT 104, E. coli CECT 432, E. coli CECT 543, E. coli CECT 877 and Shigella sonnei CECT 542 were sensitive, while seven other E. coli strains as well as Salmonella choleraesuis CECT 722, S. choleraesuis CECT 916, Enterobacter cloacae CECT 194 and Aeromonas hydrophila CECT 398 were resistant.
authors: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors: Hikmate
authors: H
authors: Abriouel
authors: Eva
authors: E
authors: Valdivia
authors: Manuel
authors: M
authors: Martínez-Bueno
authors: Mercedes
authors: M
authors: Maqueda
authors: Antonio
authors: A
authors: Gálvez
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification; D002850:Chromatography, Gel; D013293:Enterococcus faecalis / Q000378:metabolism*; D005285:Fermentation; D005519:Food Preservation / Q000379:methods
chemical_list: D001430:Bacteriocins; C466236:enterocin AS-48, Enterococcus faecalis