title: Activation of protein-bound copper ions during early glycation: study on two proteins.
abstract: This study proposes several possible pathways by which hyperglycemia could make protein-bound metal ions more redox active. These mechanisms were tested on bovine serum albumin and calf lens protein. Proteins rich in early glycation products were less capable of competing for copper ions in the presence of other ligands (e.g., glycine and calcein), suggesting that glycated proteins might have diminished stability constants of their copper chelates compared to control counterparts. When protein-copper complexes were tested for their ability to cause the oxidation of ascorbic acid, as well as the reduction of molecular oxygen to hydrogen peroxide, glycated and control proteins differed considerably in their redox abilities. Oxidative damage on proteins documented by protein carbonyl content and amino acid analysis indicates the involvement of Fenton chemistry upon metal chelation. The possible biological consequences of the observed activation of metal ions bound to early glycated proteins are discussed.
authors: Mason Eye Institute, University of Missouri, 404 Portland, St., Columbia, MO 65201, USA. margirova@hotmail.com
authors: Mariana D
authors: MD
authors: Argirova
authors: Beryl J
authors: BJ
authors: Ortwerth
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D003459:Crystallins / Q000737:chemistry*; D004355:Drug Stability; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000138:chemical synthesis*; D007477:Ions; D010084:Oxidation-Reduction; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D003459:Crystallins; D017127:Glycation End Products, Advanced; D007477:Ions; D011506:Proteins; D012710:Serum Albumin, Bovine; D003300:Copper; D006861:Hydrogen Peroxide; D005947:Glucose