Cytomegalovirus infection in liver transplant recipients.
OBJECTIVE
To explore cytomegalovirus (CMV) infection in recipients of liver transplantation (LT).


METHODS
30 recipients of LT were screened for the appearance of CMV infection by using ELISA to test anti-CMV-Ab from serum samples and using immunohistochemistry method to test CMV antigen expression and nested-PCR to amplify CMV-DNA from blood samples.


RESULTS
Four of 243 samples taken from 30 recipients came out positive of anti-CMV IgG and anti-CMV IgM with a positive rate of 100% and 1.6% respectively. 85 samples resulted in CMV antigen expression (35.0%) with the average antigen index being 4.2+/-3.1/5 X 10(4) WBC. Besides, 99 samples were found to be positive by nested-PCR with a positive rate of 40.7%. 61 samples were found to be simultaneously positive in test of CMV antigen and DNA, with a rate of 25.1%.


CONCLUSIONS
Infection of CMV is common in recipients of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplantation is very important for early diagnosis of CMV infection.
Fan
Jun
J
Institute of Infectious Diseases and Key Laboratory of Viral Infectious Diseases, Ministry of Health, Zhejiang University School of Medicine, Hangzhou 310003, China. fjjc@yeh.net
Ma
Wei-Hang
WH
Qian
Jing
J
Chen
Zhi
Z
Huang
Dong-Sheng
DS
Wang
Wei-Lin
WL
Zheng
Shu-Sen
SS
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016031:Liver Transplantation; D008297:Male; D011183:Postoperative Complications / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M