title: Clinical significance of TGF- beta1 and beta-glucuronidase synchronous detection in human pancreatic cancer.
abstract: OBJECTIVE
  To investigate the relation of transfer growth factor (TGF-beta1) and beta-glucuronidase (beta-GCD) on the occurrence and progress of pancreatic cancer.
  
  
  METHODS
  The expression of TGF-beta1 and beta-GCD in the pancreatic cancer tissue and normal pancreatic tissue was determined synchronously using ABC method of immunohistochemistry.
  
  
  RESULTS
  The percentage of TGF-beta1 positive cells was significantly higher in pancreatic cancer tissue (43.8%+/-5.2%) than in adjacent pancreatic tissue (28.7%+/-3.6%, P<0.01). The worse the cancer cells differentiated and lymph nodes metastasis, the more over-expression of TGF-beta1. The percentage of beta-GCD positive cells was also significantly higher in the pancreatic cancer tissue (62.5%+/-4.1%) than in the adjacent pancreatic tissue (33.5%+/-2.8%, P<0.01). The degree of over-expression of beta-GCD was related to the degree of cancer cells differentiation, but not to the lymph nodes metastasis. The expression of TGF-beta1 was significantly correlated with the expression of beta-GCD in pancreatic cancer tissue.
  
  
  CONCLUSIONS
  The genesis of pancreatic cancer results from multi-factor, multi-step and multi-gene variation. The synchronous detection of TGF-beta1 and beta-GCD helps to determine the malignant degree of tumors and the prognosis of patients with such disease.
authors: Department of Digestive Diseases, People's Hospital, Wuhan University, Wuhan 430071, China. kyk567@263.net
authors: Hui
authors: H
authors: Yue
authors: Bo
authors: B
authors: Yang
authors: Hong
authors: H
authors: Zhang
authors: Shan-De
authors: SD
authors: Zhu
authors: Xiao-Ju
authors: XJ
authors: Du
authors: Xin-Li
authors: XL
authors: Feng
authors: Zhong
authors: Z
authors: Yu
authors: Yu-Ting
authors: YT
authors: Xia
authors: Jie-Ping
authors: JP
authors: Yu
mesh_terms: D002454:Cell Differentiation; D018450:Disease Progression; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
chemical_list: C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D005966:Glucuronidase