Three new chlorine containing antibiotics from a marine-derived fungus Aspergillus ostianus collected in Pohnpei.
A marine bacterium Ruegeria atlantica (designated as strain TUF-D) was isolated from a glass plate submerged in the coastal water. Three new chlorine containing compounds (1 to approximately 3), together with penicillic acid (4) were obtained from a marine-derived fungus Aspergillus ostianus strain TUF 01F313 isolated from a marine sponge at Pohnpei as antibacterial components against R. atlantica. The structures of three new antibiotics were determined based on their spectral data as 8-chloro-9-hydroxy-8,9-deoxyasperlactone (1), 9-chloro-8-hydroxy-8,9-deoxyasperlactone (2), and 9-chloro-8-hydroxy-8,9-deoxyaspyrone (3). Compound 1 inhibited the growth of R. atlantica at 5 microg/disc (inhibition zone: 12.7 mm), while 2 and 3 were active at 25 microg/disc (10.1 and 10.5 mm, respectively).
Namikoshi
Michio
M
Department of Ocean Sciences, Tokyo University of Fisheries, Minato-ku, Tokyo 108-8477, Japan. namikosh@tokyo-u-fish.ac.jp
Negishi
Rika
R
Nagai
Hiroshi
H
Dmitrenok
Andrey
A
Kobayashi
Hisayoshi
H
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020561:Alphaproteobacteria / Q000187:drug effects / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001230:Aspergillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002713:Chlorine / Q000032:analysis*; D007564:Japan; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010398:Penicillic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011161:Porifera / Q000382:microbiology; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D012623:Seawater / Q000382:microbiology*
C479700:8-chloro-9-hydroxy-8,9-deoxyasperlactone; C479701:9-chloro-8-hydroxy-8,9-deoxyasperlactone; C479702:9-chloro-8-hydroxy-8,9-deoxyaspyrone; D000900:Anti-Bacterial Agents; D007783:Lactones; D011753:Pyrones; D002713:Chlorine; D015107:4-Butyrolactone; D010398:Penicillic Acid