A technique permitting selection of penicillin-susceptible pneumococcal strains following transformation of a penicillin-resistant strain with a penicillin-susceptibility gene.
We report a technique useful for transformation experiments involving bacteria naturally competent for DNA transformation. It allows the selection of antibiotic-susceptible transformants following the transformation of a resistant strain with an antibiotic susceptibility gene. We show the effectiveness of this technique through the selection of penicillin-susceptible (MIC, 0.03 microg/ml) transformants following the transformation of a penicillin-resistant (MIC, 16 microg/ml) pneumococcal strain with a penicillin-susceptibility gene.
Smith
Anthony Marius
AM
Respiratory and Meningeal Pathogens Research Unit, National Institute for Communicable Diseases, P.O. Box 1038, Corner: Hospital and De Korte Streets, Johannesburg 2000, South Africa. anthony.smith@nhls.ac.za
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D016254:Mutagenesis, Insertional / Q000379:methods; D010403:Penicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000494:pharmacology*; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
D002352:Carrier Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C072223:PBP 2x protein, Streptococcus; D004917:Erythromycin