The Tat protein of the human immunodeficiency virus type 1 (HIV-1) interacts with the EGF-like repeats of the Notch proteins and the EGF precursor.
Employing the yeast two-hybrid system, the Tat protein of the human immunodeficiency virus (HIV) was shown to interact with a region spanning the EGF-like repeats 1-6 of the mouse Notch1, the human Notch2 and the Drosophila Notch. This observation was confirmed in mammalian cells by demonstrating an interaction between the HIV Tat and the EGF-like repeats 1-6 of the various Notch proteins. The HIV Tat protein interacted also with the full-length mouse Notch1 receptor when co-expressed in mammalian cells. Moreover, the HIV Tat protein interacted also with the EGF-like repeats 1-4-spanning domain of the human EGF precursor. The ability of the HIV Tat protein to interact with the Notch proteins and possibly with other EGF-like repeats-bearing proteins, suggests that such interactions might modulate their physiological functions, thus affecting various AIDS-associated pathologies.
Shoham
Nitza
N
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
Cohen
Limor
L
Yaniv
Abraham
A
Gazit
Arnona
A
D000818:Animals; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000378:metabolism; D015696:Gene Products, tat / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D051880:Receptors, Notch; D020449:Repetitive Sequences, Amino Acid; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques
D015696:Gene Products, tat; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; C497764:NOTCH2 protein, human; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; D011498:Protein Precursors; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D014157:Transcription Factors; D004815:Epidermal Growth Factor