[Influence of KATP channel inhibitor on the changes of HSP70 expression in sevoflurane-induced neonatal rat cardiomyocytes].
OBJECTIVE
To study the roles of KATP channel and HSP70 in sevoflurane-induced preconditioning in neonatal rat cardiomyocytes and their mutual relationship.


METHODS
The second generation of primary cultured cardiomyocytes were randomly divided into 5 groups; normal control, anoxia/reoxygenation, sevoflurane preconditioning, glyburide and glyburide plus sevoflurane. In each group, the cardiomyocytes were exposed to a 2-hour anoxia, followed by a 48-hour reoxygenation. We detected HSP70 expression at 0, 1, 12, 24, 36 and 48 hours after reoxygenation respectively.


RESULTS
At each time-point of reoxygenation, the expression of HSP70 in sevoflurane preconditioning group was significantly higher than that of normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups (P < 0.01). There was no significant difference concerning HSP70 expression among normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups (P > 0.05).


CONCLUSIONS
Both HSP70 and KATP channel may be involved in the process of sevoflurane preconditioning in neonatal rat cardiomyocytes. Blocking the KATP channel can inhibit the expression of HSP70.
Tang
Ying
Y
Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Wang
Quanyun
Q
Li
Jingyi
J
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005905:Glyburide / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D019157:Ischemic Preconditioning, Myocardial; D008738:Methyl Ethers / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
D018840:HSP70 Heat-Shock Proteins; D008738:Methyl Ethers; D026902:Potassium Channel Blockers; D000077149:Sevoflurane; D000255:Adenosine Triphosphate; D005905:Glyburide