title: Prediction of nodal metastasis by comparative genomic hybridization in biopsy specimens from patients with superficial esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  Selection of appropriate protocols for treatment of superficial esophageal squamous cell carcinoma (SESCC) is dependent on lymph node metastasis status. Therefore, it is important to know whether lymph node metastasis is present before treatment.
  
  
  METHODS
  In this study, we examined the relation between DNA sequence copy number aberrations detected by comparative genomic hybridization and lymph node metastasis in 26 surgically resected SESCCs (training samples). We then assessed whether the genetic information is predictive for nodal status in biopsy specimens from eight newly enrolled patients with SESCC (blinded samples).
  
  
  RESULTS
  Pathological examination revealed that 17 of 26 training samples (65.4%) did not have associated lymph node metastasis. Gains of 8q24 and/or 20q12-qter were observed in 12, including all (nine of nine) with nodal metastasis. Fourteen training samples did not have gain of either 8q24 or 20q12-qter. Of the blinded samples, two showed no gain of 8q24 or 20q12-qter, and as anticipated the postoperative pathological examination revealed no nodal metastasis. The remaining six blinded samples had gains of 8q24 and/or 20q12-qter, and lymph node metastasis was detected by postoperative examination in four of these tumors.
  
  
  CONCLUSIONS
  Absence of gains of 8q24 and/or 20q12-qter appears to be associated with absence of lymph node metastasis in patients with SESCC; therefore, less invasive surgery can be chosen.
authors: Department of Pathology, Yamaguchi University School of Medicine, Ube 755-8505, Yamaguchi, Japan.
authors: Takashi
authors: T
authors: Ueno
authors: Akira
authors: A
authors: Tangoku
authors: Shigefumi
authors: S
authors: Yoshino
authors: Toshihiro
authors: T
authors: Abe
authors: Hideto
authors: H
authors: Hayashi
authors: Hiroaki
authors: H
authors: Toshimitsu
authors: Kiichiro
authors: K
authors: Hashimoto
authors: Tomomitsu
authors: T
authors: Satoh
authors: Atsunori
authors: A
authors: Oga
authors: Tomoko
authors: T
authors: Furuya
authors: Masaaki
authors: M
authors: Oka
authors: Kohsuke
authors: K
authors: Sasaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011182:Postoperative Care; D011237:Predictive Value of Tests
chemical_list: D004273:DNA, Neoplasm