Regulated binding of the fibrinogen-like domains of tenascin-R and tenascin-C to the neural EGF family member CALEB.
The neural transmembrane protein CALEB was discovered in a screen for novel molecules implicated in neuronal differentiation processes and was found to bind to two proteins of the extracellular matrix, tenascin-C and tenascin-R. The expression of different isoforms of CALEB in axon- and synapse-rich areas in the nervous system is regulated during development. Here we show that an unusual acidic peptide segment of CALEB is sufficient to mediate the binding of CALEB to the fibrinogen-like globes of both tenascin family members as well as to native tenascin-C. We identify a small sequence element within the acidic peptide segment of CALEB as important for this binding. Interestingly, the interactions of CALEB and tenascin-C and -R seem to be regulated during development. We demonstrate that only CALEB-80, the expression of which is up-regulated in the chicken retina during synaptogenesis, but not CALEB-140, expressed later on in development, can bind to the fibrinogen-like domains of tenascin-R or tenascin-C and to native tenascin-C. While both CALEB-80 and CALEB-140 are expressed in the plexiform layers and the optic fiber layer of embryonic chicken retina, CALEB-140 labeling is more intense in the optic fiber layer in comparison to the inner plexiform layer.
Schumacher
Stefan
S
Institut für Zellbiochemie und Klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. sschumac@uke.uni-hamburg.de
Stübe
Eva-Maria
EM
D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D019556:COS Cells; D002642:Chick Embryo; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005340:Fibrinogen / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008863:Microspheres; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D012160:Retina / Q000196:embryology / Q000378:metabolism*; D019063:Tenascin / Q000235:genetics / Q000378:metabolism*
D030161:Avian Proteins; C104596:CALEB protein, chicken; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D019063:Tenascin; C074638:tenascin R; D004815:Epidermal Growth Factor; D005340:Fibrinogen