Evidences of high carbon catabolic enzyme activities during sporulation of Pleurotus ostreatus (Florida).
Measurements of the specific activities of the representative enzymes of different pathways linked to carbohydrate metabolism indicate that glycolysis and TCA cycles are the major route of carbohydrate catabolism in the sporulating phase of fruiting body development in Pleurotus ostreatus. Enzymes of the pentose phosphate pathway always showed lower specific activities as compared to those of the enzymes of the glycolytic pathway. The activity of NADP linked glutamate dehydrogenase which is known to be an anabolic enzyme decreased drastically in sporulating fruiting bodies and in spore containing gill tissue (spore bearing structure). Mannitol dehydrogenase activity declined significantly in the sporulating phase of P. ostreatus. The high rate of metabolism during sporulation was further supported by a lower rate of gluconeogenesis at this stage. Concentrations of all the major sugars of the fruiting body (mannitol, glucose and trehalose) decreased in the mature fruiting body and gill tissue. This indicated high catabolic activities at this stage of development.
Chakraborty
Tapas K
TK
Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata--700 032, India.
Das
Nirmalendu
N
Mukherjee
Mina
M
D050260:Carbohydrate Metabolism; D002952:Citric Acid Cycle; D005943:Gluconeogenesis; D005947:Glucose / Q000032:analysis; D018086:Glutamate Dehydrogenase (NADP+) / Q000378:metabolism; D006019:Glycolysis; D008353:Mannitol / Q000032:analysis; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry; D010427:Pentose Phosphate Pathway; D020076:Pleurotus / Q000201:enzymology* / Q000502:physiology; D013172:Spores, Fungal / Q000254:growth & development; D014199:Trehalose / Q000032:analysis
D008353:Mannitol; D014199:Trehalose; D008354:Mannitol Dehydrogenases; D018086:Glutamate Dehydrogenase (NADP+); D005947:Glucose