Alternating gene expression in fibroblasts adhering to multilayers of chitosan and dextran sulfate.
The alternating compatibility of fibroblast cells adhering to multilayers of chitosan and dextran sulfate (Dex) on a substrate was analyzed at the gene expression level. A greater number of round-shaped cells adhered to the films with an outermost surface composed of Dex, although the total number of adherent cells was independent of the film surface. Greater expression of heat-shock protein 70B mRNA was demonstrated in cells adhering to films with the Dex surface. It was found that analysis of gene expression was a powerful tool for evaluating the bioactivity of multilayers.
Serizawa
Takeshi
T
Department of Nanostructured and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
Yamaguchi
Miyuki
M
Kishida
Akio
A
Akashi
Mitsuru
M
D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D016207:Cytokines / Q000235:genetics; D016264:Dextran Sulfate / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D001672:Biocompatible Materials; D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D002686:Chitin; D048271:Chitosan; D016264:Dextran Sulfate