title: Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR.
abstract: Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0.900), whereas the plasma and PBL loads correlated poorly (r(2) = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.
authors: Department of Pathology, School of Medicine, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15213, USA. Robert.Wadowsky@chp.edu
authors: Robert M
authors: RM
authors: Wadowsky
authors: Stella
authors: S
authors: Laus
authors: Michael
authors: M
authors: Green
authors: Steven A
authors: SA
authors: Webber
authors: David
authors: D
authors: Rowe
mesh_terms: D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D019562:Viral Load
chemical_list: D004279:DNA, Viral