Comparison of PCR-enzyme-linked immunosorbent assay and real-time PCR assay for diagnosis of an unusual case of cerebral toxoplasmosis in a stem cell transplant recipient.
A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.
Menotti
Jean
J
Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Paris, France. jean.menotti@sls.ap-hop-paris.fr
Vilela
Gustavo
G
Romand
Stéphane
S
Garin
Yves Jean-François
YJ
Ades
Lionel
L
Gluckman
Eliane
E
Derouin
Francis
F
Ribaud
Patricia
P
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014122:Toxoplasma / Q000145:classification / Q000302:isolation & purification*; D016781:Toxoplasmosis, Cerebral / Q000097:blood / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G