[Role of nitric oxide in the SOS response in Escherichia coli].
Having one electron with unpaired spin, nitric oxide (NO) shows high reactivity and activates or inhibits free radical chain reactions. NO toxic and genotoxic effects appear to be the result of intracellular formation of peroxinitrite that can induce some cellular damages, including DNA strand breaks, DNA base oxidation, destruction of the key enzymes, etc. Taking into account the character of DNA damages being formed under NO activity, we proposed a formation of the SOS signal and induction the SOS DNA repair response in E. coli cells treated with NO physiological donors--DNIC and GSNO. The ability of NO donor compounds to induce the SOS DNA response in E. coli PQ37 with sfiA::lacZ operon fusion is reported here at the first time. So, the SOS DNA repair response induction is one of the function of nitric oxide.
Vasil'eva
S V
SV
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119991 Russia. svasilieva@polymer.chph.ras.ru
Stupakova
M V
MV
Lobysheva
I I
II
D019677:Artificial Gene Fusion; D004249:DNA Damage; D004269:DNA, Bacterial / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D007763:Lac Operon; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D013014:SOS Response, Genetics
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D020030:Nitric Oxide Donors; C053139:sulA protein, E coli; D009569:Nitric Oxide