Kinetics of DNA binding with chloroquine phosphate using capacitive sensing method.
The capacitive sensing method has been applied to study the binding of DNA with chloroquine phosphate. DNA was immobilized on a gold electrode surface, self-assembled with thioglycolic acid. The results of a quartz crystal impedance (QCI) study indicate that the reaction of double-strand DNA (dsDNA) with chloroquine includes a fast electrostatic attraction and a slow intercalation of chloroquine into double-strand helix. The real-time experimental data obtained by capacitive sensing also revealed two distinctive kinetics stages during binding of dsDNA with chloroquine, while only one stage exists during reaction of single-strand DNA (ssDNA) with chloroquine. The kinetic parameters were obtained by fitting the real-time experimental data using a two stage reaction model. The rate constants of electrostatic attraction for dsDNA and ssDNA are estimated as 0.014 and 0.018 s(-1), respectively. The rate constant of the second stage of dsDNA is 0.0011 s(-1).
Yin
Fan
F
State Key Laboratory of Chemo/Biological Sensing and Chemometrics, Hunan University, Changsha 410082, China.
Guo
Manli
M
Yao
Shouzhuo
S
D000327:Adsorption; D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002738:Chloroquine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D007700:Kinetics; D046911:Macromolecular Substances; D009862:Online Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D046911:Macromolecular Substances; C023676:chloroquine diphosphate; D002738:Chloroquine; D004247:DNA