Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions.
The Burkholderia pseudomallei rpoS gene was identified, and an rpoS null mutant was constructed. The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase.
Subsin
Benchamas
B
Department of Biochemistry, Faculty of Science, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Thomas
Mark S
MS
Katzenmeier
Gerd
G
Shaw
Jonathan G
JG
Tungpradabkul
Sumalee
S
Kunakorn
Mongkol
M
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D016957:Burkholderia pseudomallei / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D002244:Carbon; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D018384:Oxidative Stress; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D014774:Virulence
D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D002244:Carbon