title: Involvement of semenogelin-derived peptides in the antibacterial activity of human seminal plasma.
abstract: Mechanisms for protecting spermatozoa, and the testes that produce them, from infection are essential, given the importance of these cells and organs for the fertility of the individual and perpetuation of the species. This is borne out by the publication of numerous papers on this subject over the last 50 years. We extended our work and that of others on the anti-infectious defense system of the male genital tract, using a new strategy for the direct identification of antibacterial molecules in human seminal plasma. We subjected a liquefied seminal plasma cationic fraction to reversed-phase HPLC, monitored microbicidal activity by gel overlay and radial diffusion assays, and identified the proteins and/or peptides present in each active fraction by mass spectrometry. In addition to proteins with known potent microbicidal activity--phospholipase A2, lactoferrin, and lysozyme--we also found that peptides produced by cleavage of semenogelin I, the predominant human semen coagulum protein, had high levels of antibacterial activity.
authors: GERM-INSERM U.435, Campus de Beaulieu, Université de Rennes I, 35042 Rennes Cedex, Bretagne, France.
authors: Frédéric
authors: F
authors: Bourgeon
authors: Bertrand
authors: B
authors: Evrard
authors: Michèle
authors: M
authors: Brillard-Bourdet
authors: Daniel
authors: D
authors: Colleu
authors: Bernard
authors: B
authors: Jégou
authors: Charles
authors: C
authors: Pineau
mesh_terms: D000595:Amino Acid Sequence; D002412:Cations; D002458:Cell Fractionation; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D004927:Escherichia coli Infections / Q000276:immunology*; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D012661:Semen / Q000276:immunology* / Q000382:microbiology*; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002412:Cations; D010446:Peptide Fragments; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D010741:Phospholipases A; D054467:Phospholipases A2; D009113:Muramidase; D007781:Lactoferrin