title: [Expression of androgen receptor isoforms in normal human prostate].
abstract: OBJECTIVE
  To investigate the expression of androgen receptor (AR) isoforms in normal human prostate.
  
  
  METHODS
  Fourteen normal prostatic specimens from donors, aged 25 on average (21-28 yr), were analyzed by high resolution isoelectric focusing (IEF). The expression of AR isoforms was demonstrated in all 14 normal human prostatic tissues.
  
  
  RESULTS
  Four types of AR isoforms were detected with isoelectric point value at 6.5, 6.0, 5.8 and 5.3 in 14 prostatic specimens. Binding of 3H-dihydrotestosterone (DHT) to these four AR isoforms was inhibited by the addition of 100-fold excess of DHT and testosterone. No effect of progesterone, estradiol and diethylstilbestrol on tritiated hormone binding was observed.
  
  
  CONCLUSIONS
  There are four AR isoforms in normal human prostate. The expression of AR isoforms is different from one another.
authors: Department of Urology, Shanghai First People's Hospital, Jiaotong University, Shanghai 200080, China.
authors: Shu-Jie
authors: SJ
authors: Xia
authors: Gang-Yue
authors: GY
authors: Hao
authors: Wei
authors: W
authors: Wen
authors: Jie
authors: J
authors: Zhang
authors: Wei-Guo
authors: WG
authors: Li
authors: Yi-Yong
authors: YY
authors: Zhu
authors: Xiao-Da
authors: XD
authors: Tang
mesh_terms: D000328:Adult; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D011467:Prostate / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis; D011944:Receptors, Androgen / Q000096:biosynthesis*
chemical_list: D020033:Protein Isoforms; D011944:Receptors, Androgen