[Activation of transcription factors induced by low concentration of N-methyl-N'-nitro-N-nitrosoguanidine].
To investigate the effect of MNNG on some of the transcription factors such as NF- kappaB, CREB, AP-1 and c-Myc.



The activities of these transcription factors were measured by transient transfection assay of SEAP vectors.



The expressions of AP-1, CREB and NF- kappaB driven reporter genes were elevated for about 1.3, 1.4 and 1.3 times in MNNG-treated cells, respectively, as compared to untreated controls. However, the exposure of MNNG had no effect on the activity of c-Myc.



The activation of certain transcription factors might be involved in the process of untargeted mutation induced by low concentration of MNNG treatment.
Wang
Gu-liang
GL
Department of Pathophysiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Wang
Zheng
Z
Yang
Jun
J
Yu
Ying-nian
YN
D000818:Animals; D002522:Chlorocebus aethiops; D017362:Cyclic AMP Response Element-Binding Protein / Q000187:drug effects; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009154:Mutation; D016328:NF-kappa B / Q000187:drug effects; D016271:Proto-Oncogene Proteins c-myc / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000187:drug effects; D014157:Transcription Factors / Q000187:drug effects*; D014709:Vero Cells
D017362:Cyclic AMP Response Element-Binding Protein; D016328:NF-kappa B; D016271:Proto-Oncogene Proteins c-myc; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D008769:Methylnitronitrosoguanidine