Radiation protective effect of an extract from Chelidonium majus.
We earlier reported that CM-AIa isolated from Chelidonium majus had mitogenic activity, generated lymphokine-activated killer cells, and increased the number of granulocyte-macrophage colony-forming cells (GM-CFC). In an extended effort to search for other immunostimulatory effects, we evaluated the protective effects of in vivo injected CM-AIa against irradiation. CM-AIa was found to increase the number of bone marrow cells, spleen cells, GM-CFC, and platelets in irradiated mice. In addition, this agent induced endogenous production of cytokines such as interleukin 1 and tumor necrosis factor alpha, which are required for hematopoietic recovery. We also demonstrated that CM-AIa treatment 24 hours before irradiation protected mice with 80% survival at lethal dose 100/15. These findings indicate that CM-AIa may be a useful agent for reducing the time needed for reconstitution of hematopoietic cells after irradiation treatment.
Song
Jie-Young
JY
Laboratory of Immunology, Korea Institute of Radiological and Medical Sciences, KAERI, Seoul, Korea.
Yang
Hyun-Ok
HO
Shim
Ji-Young
JY
Ji-Yeon-Ahn
Han
Young-Soo
YS
Jung
In-Sung
IS
Yun
Yeon-Sook
YS
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D028281:Chelidonium / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D006413:Hematopoietic System / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D007107:Immune System / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000401:mortality
D016207:Cytokines; D010936:Plant Extracts; D011837:Radiation-Protective Agents