Peroxynitrite scavenging activity of lithospermate B from Salvia miltiorrhiza.
Peroxynitrite (ONOO-) is produced by the reaction of superoxide (O2-) with nitric oxide. ONOO- damages proteins through nitration or oxidation. For protection from ONOO- induced protein modifications, ONOO- scavengers should be supplemented. Evidence was obtained that lithospermate B extracted from Salvia miltiorrhiza showed the strongest scavenging activity among its constituents. Its ONOO- scavenging activity is via an electron donation mechanism. A dihydroxyl group and a double bond seem to be essential structure requirements. The data from the experiments further confirmed a protective effect of lithospermate B on bovine serum albumin and low-density lipoprotein against ONOO-. This study demonstrated that lithospermate B with hydroxyl groups and double bonds exerts an anti-nitration effect by scavenging ONOO-.
Soung
Do You
DY
Department of Pharmacy, Pusan National University, Busan, Korea.
Rhee
Sook Hee
SH
Kim
Jung Sun
JS
Lee
Ji Young
JY
Yang
Hee-Sun
HS
Choi
Jae Sue
JS
Yokozawa
Takako
T
Han
Yong Nam
YN
Chung
Hae Young
HY
D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; C061091:lithospermate B; D030421:Peroxynitrous Acid; D012710:Serum Albumin, Bovine