[Effect of daphnetin on SOD activity and DNA synthesis of Plasmodium falciparum in vitro].
OBJECTIVE
To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro.


METHODS
The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P.f) FCC1 in vitro was determined with a SOD test-kit. The level of DNA synthesis of P.f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258.


RESULTS
The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P.f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P.f trophozoites in synchronized in vitro culture was significantly lower than that of the control.


CONCLUSIONS
Daphnetin lowered SOD activity and decreased DNA synthesis of P.f in vitro.
Mu
Ling-yun
LY
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Wang
Qin-mei
QM
Ni
Yi-chang
YC
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000096:biosynthesis*; D003676:Deferoxamine / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014468:Umbelliferones / Q000494:pharmacology*
D000962:Antimalarials; D016054:DNA, Protozoan; D014468:Umbelliferones; D013482:Superoxide Dismutase; D003676:Deferoxamine; C039952:daphnetin