Osteoblast recruitment from stem cells does not decrease by age at late adulthood.
This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood.
Leskelä
Hannu-Ville
HV
Department of Anatomy and Cell Biology, University of Oulu, PL 5000, FIN-90014, Oulu, Finland. haleskel@paju.oulu.fi
Risteli
Juha
J
Niskanen
Salla
S
Koivunen
Jussi
J
Ivaska
Kaisa K
KK
Lehenkari
Petri
P
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001846:Bone Development / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D012737:Sex Factors
D024042:Collagen Type I; D024061:Collagen Type III; D015675:Osteocalcin; D000469:Alkaline Phosphatase