E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma.
OBJECTIVE
To study the significance of E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma (HCC).


METHODS
Flow cytometry and immunohistochemistry were used to detect the expression of E-selectin and its ligand-sLeX in both HCC cell lines and human HCC tissues.


RESULTS
The positive rate of E-selectin in vascular endothelial cells adjacent to cancer was 67.9% (19/28). The expression of E-selectin in tumors accompanied with emboli or satellite foci was significantly higher than that in tumors without emboli or satellite foci (P<0.05), and it was not related to tumor size, tumor capsule, AFP content, and the degree of differentiation. The positive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGII cell lines was 7.03%, 63.35% and 97.29% respectively. The positive cells of sLeX mainly distributed in the margin of tumor tissues. The positive expression of sLeX in HCC cells in emboli or invasive tumor tissues was much higher than in Primary foci.


CONCLUSIONS
E-selectin and its ligand-sLeX are closely correlated with the metastasis of HCC.
Zhang
Bao-Hua
BH
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University of PLA, Shanghai 200438, China. zhbh_1@163.net
Chen
Han
H
Yao
Xiao-Ping
XP
Cong
Wen-Ming
WM
Wu
Meng-Chao
MC
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000556:secondary*; D019040:E-Selectin / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D009844:Oligosaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
D019040:E-Selectin; D008024:Ligands; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen