Screening of entomopathogenic Deuteromycetes for activities on targets involved in degenerative diseases of the central nervous system.
A selection of 32 fungal strains, belonging to 8 genera of entomopathogenic Deuteromycetes collected in various provinces of China, were screened for activities on targets involved in degenerative diseases of the central nervous system. The strains were grown under various fermentation conditions, and a total of 256 different extracts were obtained. The bioassays included functional screens for NMDA antagonistic activity in stably transfected fibroblasts, for neuritogenic activities in PC-12 cells, and tests for MAO inhibitory and radical scavenging properties. Several extracts with promising activities were identified. Some Paecilomyces extracts induced pronounced axonal-like outgrowths in PC-12 cells. In Paecilomyces militaris RCEF 0095, the neuritogenic activity could be linked to yellow pigments. Three Beauveria and Paecilomyces strains showed radical scavenging properties, which could be localized in the extract by a bioautographic assay on TLC. An extract obtained from the mycelium of Paecilomyces tenuipes RCEF 0275 showed moderate MAO inhibitory activity, whereas extracts of Sporothrix chondracris RCEF 0187 antagonized NMDA receptor mediated cell toxicity.
Schmidt
K
K
Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, Jena D-07743, Germany.
Li
Z
Z
Schubert
B
B
Huang
B
B
Stoyanova
S
S
Hamburger
M
M
D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D002470:Cell Survival; D002478:Cells, Cultured; D002491:Central Nervous System Agents / Q000627:therapeutic use*; D002493:Central Nervous System Diseases / Q000188:drug therapy*; D002855:Chromatography, Thin Layer; D004353:Drug Evaluation, Preclinical; D005285:Fermentation; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D016166:Free Radical Scavengers / Q000627:therapeutic use; D008930:Mitochondria, Liver / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D008995:Monoamine Oxidase / Q000378:metabolism; D051381:Rats
D000935:Antifungal Agents; D002491:Central Nervous System Agents; D016166:Free Radical Scavengers; D008995:Monoamine Oxidase