Absorption of sugars and amino acids by the epidermis of Aphidius ervi larvae.
Aphidius ervi Haliday (Hymenoptera, Braconidae) is an endophagous parasitoid of several aphid species of economic importance, widely used in biological control. The definition of a suitable artificial diet for in vitro mass production of this parasitoid is still an unresolved issue that, to be properly addressed, requires a deeper understanding both of its nutritional needs and of the functional properties of the larval epithelia involved in nutrient absorption. The experimental evidence presented in this paper unequivocally demonstrates that the uptake of sugars and amino acids takes place through the body surface of the larval stages of A. ervi. These nutrients are efficiently absorbed by the larval epidermis, but the transport rate progressively declines over time. The epidermis exhibits a cross-reactivity to antibodies raised against the mammalian facilitative glucose transporter GLUT2 and the sodium cotransporter SGLT1. The analysis of sugar transport sensitivity to specific inhibitors indicates the involvement of GLUT2-like transporters, while a role for SGLT1-like transporters is not supported. The peculiar pathways of nutrient absorption in A. ervi larvae further corroborate the general idea that the pre-imaginal stages of endophagous koinobiont Hymenoptera, like Metazoan parasites, show a high degree of physiological integration with their hosts.
Giordana
B
B
Dipartimento di Biologia, Università di Milano, via Celoria 26, 20133 Milan, Italy.
Milani
A
A
Grimaldi
A
A
Farneti
R
R
Casartelli
M
M
Ambrosecchio
M R
MR
Digilio
M C
MC
Leonardi
M G
MG
de Eguileor
M
M
Pennacchio
F
F
D000042:Absorption; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001042:Aphids / Q000254:growth & development / Q000378:metabolism*; D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003572:Cytochalasins / Q000494:pharmacology; D004817:Epidermis / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005632:Fructose / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051273:Glucose Transporter Type 2; D006434:Hemocytes / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D007814:Larva / Q000378:metabolism / Q000648:ultrastructure; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000378:metabolism; D010693:Phloretin / Q000494:pharmacology; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism
D000596:Amino Acids; D003572:Cytochalasins; D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D005632:Fructose; D012964:Sodium; D010695:Phlorhizin; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D010693:Phloretin