title: Restitution of the bullfrog gastric mucosa is dependent on a DIDS-inhibitable pathway not related to HCO3- ion transport.
abstract: This study was conducted to determine the contribution of ion transport to restitution after injury in the gastric mucosa. For this, intact sheets of stomach from the bullfrog, Rana catesbeiana, were mounted in Ussing chambers. Restitution was evaluated in the presence or absence of ion transport inhibitors amiloride, DIDS, and bumetanide to block Na(+)/H(+) exchange, Cl(-)/HCO(3)(-) exchange and Na(+)/HCO(3)(-) co-transport, and Na(+)-K(+)-2Cl(-) cotransport, respectively. Ion substitution experiments with Na(+)-free, Cl(-)-free, and HCO(3)(-)-free solutions were also performed. Injury to the mucosa was produced with 1 M NaCl, and restitution was evaluated by recovery of transepithelial resistance (TER), mannitol flux, and morphology. Amiloride, bumetanide, Cl(-)-free, or HCO(3)(-)-free solutions did not affect restitution. In Na(+)-free solutions, recovery of TER and mannitol flux did not occur because surface cells did not attach to the underlying basement membrane. In contrast, all aspects of restitution were inhibited by DIDS, a compound that inhibits Na(+)-dependent HCO(3)(-) transport. Because HCO(3)(-)-free solutions did not inhibit restitution, it was concluded that DIDS must block a yet undefined pathway not involved in HCO(3)(-) ion transport but essential for cell migration after injury and restitution in the gastric mucosa.
authors: Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. shagen@bidmc.harvard.edu
authors: Susan J
authors: SJ
authors: Hagen
authors: Sarah W
authors: SW
authors: Morrison
authors: Christina S
authors: CS
authors: Law
authors: David X
authors: DX
authors: Yang
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D002021:Buffers; D002034:Bumetanide / Q000494:pharmacology; D002465:Cell Movement; D027963:Chloride-Bicarbonate Antiporters / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004232:Diuretics / Q000494:pharmacology; D004594:Electrophysiology; D004848:Epithelium / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D006531:HEPES / Q000494:pharmacology; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects*; D008353:Mannitol / Q000378:metabolism; D011892:Rana catesbeiana; D015398:Signal Transduction / Q000187:drug effects*; D012964:Sodium / Q000502:physiology; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D002021:Buffers; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D004232:Diuretics; D017923:Sodium-Hydrogen Exchangers; D028021:Sodium-Potassium-Chloride Symporters; D002034:Bumetanide; D008353:Mannitol; D000584:Amiloride; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D006531:HEPES