Up-regulation of microtubule-associated protein 4 and drebrin A mRNA expression by antidepressants in rat hippocampus following chronic stress.
We used the differential display-polymerase chain reaction (DD-PCR) protocol to identify genes related to antidepressant effects. Rats were subjected to different kinds of stress for 20 days, and then the antidepressants desipramine and fluoxetine were administered (10 mg/kg per day, i.p.) for 20 days. DD-PCR was performed and differentially expressed cDNAs were further confirmed by dot-blot hybridization. cDNA homology analysis revealed that desipramine up-regulated the expression of microtubule-associated protein 4 (MAP-4) mRNA and fluoxetine up-regulated the expression of drebrin A mRNA in the rat hippocampus compared with the chronically stressed group. This result suggests that MAP-4 and drebrin may be involved in the antidepressant like effects of desipramine and fluoxetine.
Yang
Hong-Ju
HJ
Beijing Institute of Pharmacology and Toxicology, Taiping Road 27, Beijing 100850, China. yanghongju618@sina.com
Li
Yun-Feng
YF
Zhang
Hang-Ting
HT
Zhang
Feng-Qin
FQ
Zhao
Nan
N
Gong
Zheng-Hua
ZH
Luo
Zhi-Pu
ZP
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D002908:Chronic Disease; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects* / Q000502:physiology
D000928:Antidepressive Agents; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; C053386:drebrins