title: Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection.
abstract: This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
authors: Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom.
authors: Rita
authors: R
authors: Sousa-Nunes
authors: Amer Ahmed
authors: AA
authors: Rana
authors: Ross
authors: R
authors: Kettleborough
authors: Joshua M
authors: JM
authors: Brickman
authors: Melanie
authors: M
authors: Clements
authors: Alistair
authors: A
authors: Forrest
authors: Sean
authors: S
authors: Grimmond
authors: Philip
authors: P
authors: Avner
authors: James C
authors: JC
authors: Smith
authors: Sally L
authors: SL
authors: Dunwoodie
authors: Rosa S P
authors: RS
authors: Beddington
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000096:biosynthesis; D004707:Endoderm / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015723:Gene Library; D051379:Mice / Q000196:embryology* / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018076:DNA, Complementary