The use of hydrogen peroxide in the experimental therapy of cysts. An in vitro analysis.
OBJECTIVE
Aim of the authors is to evaluate in vitro the histological alteration caused by hydrogen peroxide on a sample of radicular cysts.


METHODS
Twelve radicular cysts were divided into 2 portions. The control group was histologically evaluated whereas the other portion, test, was treated with 3% hydrogen peroxide before histological analysis. Test and control portions were compared for integrity of the epithelium, inflammatory state of the cystic walls, presence of necrosis, stromal vacuolization and microvessel density (MVD). MVD was evaluated by immunohistochemistry using anti-CD34 monoclonal antibodies.


RESULTS
Significantly lower epithelium integrity has been detected in the test group (p= or <0.007), (Wilcoxon test). Moreover, the test group presented a higher number of stromal vacuolization areas (p= or <0.004). However, necrosis and inflammation reported no significative differences. No significative differences were noticed for the MVD.


CONCLUSIONS
Hydrogen peroxide showed a cytopathic and cytolytic activity in vitro. Those effects have been shown on both cysts tissues: epithelium and connective tissue. The present study did not reveal any statistically significant difference between the number of vessels in the control and in the test group. However, further in vitro and human studies are needed to validate this substance in the treatment of cysts.
Graziani
F
F
Section of Odontostomatologic Special Surgery, Department of Neurosciences, University of Pisa, Pisa, Italy.
Vano
M
M
Tartaro
G
G
Fanelli
G
G
Gabriele
M
M
D001808:Blood Vessels / Q000187:drug effects / Q000473:pathology; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D007249:Inflammation; D009336:Necrosis; D011842:Radicular Cyst / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D014617:Vacuoles / Q000648:ultrastructure
D006861:Hydrogen Peroxide