Immunoreactivity of brain-derived neurotrophic factor in rat dorsal root ganglion and spinal cord dorsal horn following exposure to herniated nucleus pulposus.
The pain mechanisms underlying radiculopathy due to disc herniation are still incompletely understood. This study assessed changes in brain-derived neurotrophic factor (BDNF) expression, a modulator of nociceptive information, in the dorsal root ganglion (DRG) and spinal cord dorsal horn following experimental disc herniation. Immunohistochemical analysis revealed an increase in percentage of BDNF-immunoreactive (IR) neurons profiles in the affected DRG and marked elevation in the BDNF-IR regions within both the superficial and deep layers at the corresponding spinal level with a peak at 3 days after nucleus pulposus (NP) application. These results thus show that herniated NP increases the BDNF production in the pain-processing neurons. Such changes can contribute to the development of inflammatory hyperalgesia.
Onda
Akira
A
Department of Orthopaedics, Sahlgrenska University Hospital, Göteborg University, SE-413 45 Gothenburg, Sweden.
Murata
Yasuaki
Y
Rydevik
Björn
B
Larsson
Karin
K
Kikuchi
Shinichi
S
Olmarker
Kjell
K
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005727:Ganglia, Spinal / Q000737:chemistry / Q000378:metabolism*; D007120:Immunochemistry; D007405:Intervertebral Disc Displacement / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019208:Brain-Derived Neurotrophic Factor