Molecular cloning and chromosomal assignment to SSC12p13-->p11 of swine chemokine receptor CCR7.
We cloned a gene encoding the swine chemokine (C-C motif) receptor 7 (CCR7) and clarified its genomic structure and chromosomal assignment. The ORF and deduced amino-acid sequence were highly conserved with human and mouse CCR7. The swine CCR7 gene was mapped to SSC12p13-->p11 by FISH analysis. Stimulation of swine peripheral blood mononuclear cells by IL-12 and IL-18, considered potent inducers of Th1 cells from analyses in humans and mice, downregulated the expression of CCR7. This is the first report of the molecular cloning, chromosomal assignment and characterization of a chemokine receptor in swine.
Shinkai
H
H
Second Research Division, STAFF-Institute, Kamiyokoba, Tsukuba, Ibaraki, Japan.
Muneta
Y
Y
Eguchi
T
T
Suzuki
K
K
Awata
T
T
Uenishi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D040461:Gene Components; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000276:immunology
C515370:CCR7 protein, human; C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7; D019707:Receptors, Chemokine