Evaluation of expression patterns of feline CD28 and CTLA-4 in feline immunodeficiency virus (FIV)-infected and FIV antigen-induced PBMC.
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also examined with fresh and antigen-stimulated PBMC. FIV infection induced a lower expression of CD28, but a higher expression of CTLA-4 in the infected PBMC than in the uninfected PBMC. Relatively high levels of CD28 expression were demonstrated in both the fresh and the antigen-stimulated PBMC. The expression level of CTLA-4 in the freshly isolated PBMC was rather low, however, FIV antigen stimulation induced a relatively high expression of CTLA-4 in feline PBMC.
Choi
I S
IS
Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
Yoo
H S
HS
Collisson
E W
EW
D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D018106:CD28 Antigens / Q000096:biosynthesis*; D060908:CTLA-4 Antigen; D002415:Cats; D002470:Cell Survival; D002478:Cells, Cultured; D015870:Gene Expression; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000956:Antigens, Viral; D018106:CD28 Antigens; D060908:CTLA-4 Antigen