Single cell technology.
Complexity is a fundamental feature of life. Like animals, higher plants consist of a multitude of different distinct tissues and cell types, each contributing to the overall performance of the whole organism. Our understanding and knowledge of physiology will greatly increase as our ability to spatially resolve molecular and biochemical processes improves. Differential analysis of individual tissues and single cells will eliminate the averaging effect and allow the discovery of detailed differences between various cell types. Recent breakthroughs have been made in tissue-specific DNA, RNA and protein analysis of plants by applying laser-based microdissection techniques.
Kehr
Julia
J
Max Planck Institute of Molecular Plant Physiology, Department Willmitzer, Am Mühlenberg 1, 14476 Golm/Potsdam, Germany. kehr@mpimp-golm.mpg.de
D018744:DNA, Plant / Q000032:analysis; D042282:Microdissection / Q000295:instrumentation / Q000379:methods*; D059828:Plant Cells; D010940:Plant Proteins / Q000032:analysis; D010944:Plants / Q000737:chemistry / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant