title: An in vivo model of autoimmune post-coxsackievirus B3 myocarditis in severe combined immunodeficiency mouse.
abstract: OBJECTIVE
  Severe combined immunodeficiency (SCID) mice possess neither T nor B lymphocytes and are thus suitable recipients for adoptively transferred lymphocytes. Because autoimmune mechanisms may be involved in the pathogenesis of coxsackievirus B3 (CB3) myocarditis, we attempted to assess the in vitro cellular damage caused by antigen-sensitized lymphocytes and to determine whether splenic lymphocytes from BALB/c mice with chronic CB3 myocarditis could cause myocarditis into SCID mice.
  
  
  RESULTS
  Cytotoxic cellular damage against non-myocytes and myocytes was demonstrated using 51Cr-release assay by lymphocytes cultured from myocarditis specimens or splenocytes from mice with chronic CB3 myocarditis. Severe T lymphocyte infiltration with myocardial necrosis was found in adoptively splenocyte-transferred SCID mice, but myocardial necrosis was not defected in wild-type mice by the same procedure.
  
  
  CONCLUSIONS
  Autoimmune mechanisms might operate in mice with post-CB3 myocarditis and could be transferred in to SCID mice by the antigen-sensitized lymphocytes. An in vivo model of autoimmune post-viral myocarditis in SCID mouse was demonstrated.
authors: The Second Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan. kkishi@kuhp.kyoto-u.ac.jp
authors: Chiharu
authors: C
authors: Kishimoto
authors: Yuji
authors: Y
authors: Hiraoka
authors: Nami
authors: N
authors: Takamatsu
authors: Hitoshi
authors: H
authors: Takada
authors: Hajime
authors: H
authors: Kamiya
authors: Hiroshi
authors: H
authors: Ochiai
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases; D003384:Coxsackievirus Infections / Q000276:immunology*; D004195:Disease Models, Animal; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009205:Myocarditis / Q000276:immunology* / Q000821:virology*; D032383:Myocytes, Cardiac / Q000276:immunology / Q000473:pathology / Q000821:virology; D009336:Necrosis