title: [Effects of quercetin on platelet-endothelial cell adhesion and their expression of adhesion molecules].
abstract: OBJECTIVE
  To observe the effect of quercetin (Que) on the adhesion of platelets to cultured endothelial cells and adhesion molecule expression by human umbilical vein endothelial cells (HUVEC) and platelets.
  
  
  METHODS
  [3H]-Adenine labeled platelets were incubated with HUVEC to investigate the effect of Que on adhesion of platelets to HUVEC. The number of platelets adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy. TNF-alpha induced HUVEC expression ICAM-1 and thrombin induced platelets expression of P-selectin were measured by flow cytometry.
  
  
  RESULTS
  Que (0.3-2.4 mumol.L-1) was shown to inhibit the increase of P-selectin expression of thrombin activated platelets. Pretreatment of HUVEC with tumor necrosis factor (TNF-alpha) significantly increased platelets adhesion to HUVEC and the expression ICAM-1. Que (0.6-2.4 mumol.L-1) inhibited this effect of TNF-alpha in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Que can inhibit the adhesion of platelets to HUVEC and the expression of adhesion molecules (P-selectin and ICAM-1).
authors: Department of Pharmacology, Suzhou University Medical College, Suzhou, 215007 China. Liangzhq@etang.com
authors: Zhong-qin
authors: ZQ
authors: Liang
authors: Yi
authors: Y
authors: Zhu
authors: Zhen-lun
authors: ZL
authors: Gu
authors: Qun
authors: Q
authors: Lu
authors: Pan-sheng
authors: PS
authors: Fan
mesh_terms: D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1; D011794:Quercetin