title: Transcriptional profiling of ubp10 null mutant reveals altered subtelomeric gene expression and insurgence of oxidative stress response.
abstract: UBP10 codes for a deubiquitinating enzyme of Saccharomyces cerevisiae whose loss of function determines slow growth rate and partial impairment of silencing at telomeres and HM loci. A genome-wide analysis performed on a ubp10 disruptant revealed alterations in expression of subtelomeric genes together with a broad change in the whole transcriptional profile, closely parallel to that induced by oxidative stress. This response was accompanied by intracellular accumulation of reactive oxygen species as well as by DNA fragmentation and phosphatidylserine externalization, two markers of apoptosis. SIR4 inactivation mitigated the wide transcriptome remodeling of the ubp10 null mutant affecting particularly the stress transcriptional profile. Moreover, the ubp10sir4 disruptant did not display apoptotic markers. These results argue in favor of an involvement of deubiquitination in transcriptional control and suggest a linkage between oxidative stress and apoptotic pathway in budding yeast.
authors: Università degli Studi di Milano-Bicocca, Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Ivan
authors: I
authors: Orlandi
authors: Maurizio
authors: M
authors: Bettiga
authors: Lilia
authors: L
authors: Alberghina
authors: Marina
authors: M
authors: Vai
mesh_terms: D017209:Apoptosis; D015152:Blotting, Northern; D053938:DNA Fragmentation; D005786:Gene Expression Regulation; D016681:Genome, Fungal; D008856:Microscopy, Fluorescence; D009154:Mutation; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010641:Phenotype; D010718:Phosphatidylserines / Q000737:chemistry; D020543:Proteome; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D004718:Saccharomycetales; D015398:Signal Transduction; D016615:Telomere / Q000648:ultrastructure; D014158:Transcription, Genetic; D043222:Ubiquitin Thiolesterase
chemical_list: D009687:Nuclear Proteins; D010718:Phosphatidylserines; D020543:Proteome; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; C403282:UBP10 protein, S cerevisiae; D043222:Ubiquitin Thiolesterase