title: HCN channels contribute to the intrinsic activity of cochlear pyramidal cells.
abstract: A hyperpolarization-activated current recorded from the pyramidal cells of the dorsal cochlear nucleus was investigated in the present study by using 150- to 200-microm-thick brain slices prepared from 6- to 14-day-old Wistar rats. The pyramidal neurones exhibited a slowly activating inward current on hyperpolarization. The reversal potential of this component was -32 +/- 3 mV (mean +/- SE, n = 6), while its half-activation voltage was -99 +/- 1 mV with a slope factor of 10.9 +/- 0.4 mV (n = 27). This current was highly sensitive to the extracellular application of both 1 mM Cs+ and 10 microM ZD7288. The electrophysiological properties and the pharmacological sensitivity of this current indicated that it corresponded to a hyperpolarization-activated non-specific cationic current (Ih). Our experiments showed that there was a correlation between the availability of the h-current and the spontaneous activity of the pyramidal cells, suggesting that this conductance acts as a pacemaker current in these neurones. Immunocytochemical experiments were also conducted on freshly isolated pyramidal cells to demonstrate the possible subunit composition of the channels responsible for the genesis of the pyramidal h-current. These investigations indicated the presence of HCN1, HCN2 and HCN4 subunits in the pyramidal cells.
authors: Department of Physiology, Medical and Health Science Centre, University of Debrecen, 4012 Debrecen, Hungary.
authors: B
authors: B
authors: Pál
authors: A
authors: A
authors: Pór
authors: G
authors: G
authors: Szucs
authors: I
authors: I
authors: Kovács
authors: Z
authors: Z
authors: Rusznák
mesh_terms: D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials / Q000502:physiology; D009124:Muscle Proteins / Q000378:metabolism*; D015221:Potassium Channels; D051381:Rats
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; C578665:HCN1 protein, human; C578710:HCN2 protein, human; C119587:HCN4 protein, human; C578667:Hcn1 protein, rat; C578712:Hcn2 protein, rat; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D009124:Muscle Proteins; D015221:Potassium Channels