Reductions in bone turnover, mineral, and structure associated with mechanical properties of lumbar vertebra and femur in glucocorticoid-treated growing minipigs.
The present study was designed to determine the effects of glucocorticoid (GC) on bone turnover, minerals, structure, and bone mechanical properties in minipigs. Six 8-month-old Göttingen minipigs were subcutaneously injected with prednisolone (PN, 0.5 mg/kg body wt (BW)/day, 5 days/week for 26 weeks (Group GC)), 6 were treated with vehicle alone (Group VC), and 4 were sacrificed at start of the study for baseline controls (Group BC). The increase in BW was similar in all groups. PN significantly reduced serum osteocalcin and urinary type-1 collagen N-telopeptide levels at 13 weeks and thereafter, compared with baseline and control, and also reduced serum bone specific alkaline phosphatase levels relative to baseline. At 26 weeks, the longitudinal axis of the lumbar bone and length of femur were smaller in Group GC than Group VC. The total cross-sectional area of femur, but not the lumbar bone, in Group GC was significantly different from Group VC. BMD of the femur, but not L2, measured by DXA, was lower in Group GC than in Groups BC and VC. The cortical shell structure measured by 2D-micro-CT deteriorated and age-dependent increases in trabecular bone structure 3D micro-CT were reduced by PN. PN also caused deterioration of the cortical structure of the mid-femur. In L2 and femur, PN significantly reduced the ultimate load and maximum absorption energy of the femur and L2 compared with Group VC. The structural modulus in Group GC was lower than in Group BC. Regression analyses revealed that bone minerals, bone structure, and chemical markers correlated with mechanical properties of L2 and mid-femur. Our results indicate that PN reduced systemic bone formation and resorption and suppressed the age-dependent increases in bone minerals, structure, and mechanical properties of L2 and mid-femur. Reduced bone turnover seemed to be associated with a reduction in mechanical properties. The growing minipig could be a suitable model of GCs-induced osteoporosis in humans.
Ikeda
Satoshi
S
Department of Orthopedic Surgery, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan. s-ikeda@med.uoeh-u.ac.jp
Morishita
Yoshiomi
Y
Tsutsumi
Hideki
H
Ito
Masako
M
Shiraishi
Ayako
A
Arita
Shinobu
S
Akahoshi
Shojiro
S
Narusawa
Ken'ichiro
K
Nakamura
Toshitaka
T
D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology; D016723:Bone Remodeling / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000502:physiology; D005938:Glucocorticoids / Q000494:pharmacology; D008159:Lumbar Vertebrae / Q000187:drug effects* / Q000502:physiology; D011239:Prednisolone / Q000494:pharmacology*; D013552:Swine; D013556:Swine, Miniature
D005938:Glucocorticoids; D011239:Prednisolone