[Inhibition of MCP-1 mRNA expression by propylene glycol mannate sulfate in hyperlipidemic rat aorta].
OBJECTIVE
To study the effects of prophylene glycol mannate sulfate (PGMS) on monocyte chemoattractant protein-1 (MCP-1) mRNA expression in hyperlipidemic rat aorta and to clarify the molecular mechanism of PGMS for the prevention of atherosclerosis.


METHODS
PGMS (37.8 and 75.6 mg.kg-1.d-1, ig) or PGMS (37.8 and 75.6 mg.kg-1.d-1, ig) combined with diethyldithiocarbamate (DDC, an inhibitor of SOD, 200 mg.kg-1 every three days, i.p.) were given to hyperlipidemic rats for three weeks. The MDA content and SOD activity were determined after 12 h of starvation, and MCP-1 mRNA expression in aorta was detected by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
There was significant decrease (29.46% or 58.40)% of MCP-1 mRNA expression in aortic after the therapy. The SOD activity increased markedly and the MDA content decreased at the same time. After treatment with DDC, the SOD activity was inhibited and the MDA content increased, but with no significant effect on MCP-1 mRNA expression.


CONCLUSIONS
PGMS inhibited MCP-1 mRNA expression with no relation to its effect on decreasing MDA content.
Gao
Yan
Y
Institute of Marine Drugs and Foods, Ocean University of China, Qingdao 266003, China.
Yu
Wen-gong
WG
Han
Feng
F
Lu
Xin-zhi
XZ
Gong
Qian-hong
QH
Guan
Hua-shi
HS
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006949:Hyperlipidemias / Q000097:blood / Q000473:pathology; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D011409:Propylene Glycols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D018932:Chemokine CCL2; D000960:Hypolipidemic Agents; D011409:Propylene Glycols; D012333:RNA, Messenger; D008315:Malondialdehyde; D013482:Superoxide Dismutase