Effects of peak anomalies with the hydrophilic or hydrophobic properties of reservoirs during serial injection on a capillary electrophoresis microchip.
Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
Heo
Yun Seok
YS
School of Mechanical and Aerospace Engineering, Seoul National University, San 56-1, Shinlim-dong, Kwanak-gu, Seoul 151-742, South Korea. okhys@amed.snu.ac.kr
Chung
Seok
S
Cho
Keunchang
K
Chung
Chanil
C
Han
Dong-Chul
DC
Chang
Jun Keun
JK
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D008904:Miniaturization; D012828:Silicones / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon