[Qualitative and quantitative analysis of ammonium salts of hexamethylenetetramine and 1,10-phenanthroline].
By use of chemical and physicochemical methods the qualitative and quantitative analysis of bacteriostatic agents N-carbamoylmethylhexamethylenetetraamonium chloride (U-77) and 1-propyl-1,10-phenanthrolinium iodide (X-50) was carried out. The color reactions of these salts with various agents, e. g., concentrated acids, precipitants, oxidizers, indicators, ninhydrin, salts of heavy metals were assesssed. Some characteristic color reactions were found for analysis of quaternary ammonium salts. Experimental results indicate that interaction of N-carbamoylmethyhexamethylenetetraammonium chloride with silver nitrate leads to precipitate of the free silver in the form of a mirror under the proper conditions. It is a result of degradation of hexamethylenetetramine to formaldehyde and its oxidation, which is accompanied by reduction of silver ion to free silver. By use of thin-layer chromatography and ultraviolet spectrophotometry the physicochemical properties of compounds were tested. The suitability of qualitative methods, such as argentometry, mercurimetry, iodometry, extraction photometric analysis was detected. The results suggest, that the most suitable and precise method is argentometry.
Bernatoniene
Rūta
R
Department of Pharmaceutical Chemistry and Pharmacognosy, Kaunas University of Medicine, Kaunas, Lithuania.
Stankeviciene
Laimute
L
Stankevicius
Antanas
A
Janusiene
Laima
L
D000890:Anti-Infective Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer; D006801:Humans; D007202:Indicators and Reagents; D019216:Metals, Heavy / Q000032:analysis; D008709:Methenamine / Q000032:analysis*; D008956:Models, Chemical; D010618:Phenanthrolines / Q000032:analysis*; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D019216:Metals, Heavy; D010618:Phenanthrolines; D000644:Quaternary Ammonium Compounds; D008709:Methenamine