title: The Xenopus noggin promoter drives roof-plate specific transcription.
abstract: During vertebrate neural development, inductive signals derived from the roof plate specify dorsal neural cell types of spinal cord as well as adjacent dermomytotome. Noggin has been demonstrated to play a pivotal role in this signaling network but how its expression is regulated remains unclear. To investigate this issue, we identified a 2066 bp Xenopus noggin 5' flanking sequence which recapitulates the roof-plate expression of endogenous gene in transgenic frog tadpoles and we further mapped the roof-plate enhancer to a sequence as short as 79 bp. Our results, therefore, not only shed light on but also provide a powerful tool to the study of neural and somite patterning.
authors: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, PR China.
authors: Xin
authors: X
authors: Geng
authors: Lei
authors: L
authors: Xiao
authors: Qinghua
authors: Q
authors: Tao
authors: Ruiying
authors: R
authors: Hu
authors: Ralph A W
authors: RA
authors: Rupp
authors: Xiaoyan
authors: X
authors: Ding
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002352:Carrier Proteins; D005260:Female; D020671:Posterior Horn Cells / Q000196:embryology*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011506:Proteins / Q000235:genetics*; D019170:Somites / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics* / Q000502:physiology; D014982:Xenopus laevis; D000099176:Noggin Protein
chemical_list: D002352:Carrier Proteins; D011506:Proteins; D029867:Xenopus Proteins; D000099176:Noggin Protein