title: Efficient proliferation and adipose differentiation of human adipose tissue-derived vascular stromal cells transfected with basic fibroblast growth factor gene.
abstract: Human vascular stromal (VS) cells obtained from mature adipose tissue were transfected with an adenovirus vector carrying the basic fibroblast growth factor (bFGF) gene. bFGF protein was observed in VS cell nuclei 24 h after transfection and in the cytoplasm and extracellular space 72 h after transfection. Naive VS cells were almost static in vitro and proliferated in a dose-dependent manner on stimulation with recombinant bFGF (rbFGF). However, bFGF-transfected VS cells proliferated spontaneously to the same extent as naive VS cells when stimulated with rbFGF at 100 ng/ml. The former cells started to proliferate on day 3 after transfection and the proliferation pattern was similar to that of the latter cells, although only a slight amount of bFGF protein was detected in the culture medium when the bFGF-transfected cells started to proliferate. The proliferation of bFGF-transfected VS cells was completely inhibited by bFGF neutralizing antibody, which also completely inhibited the proliferation of naive VS cells stimulated with rbFGF. Under conditions favoring differentiation to adipocytes, bFGF-transfected VS cells stopped proliferating and started to accumulate lipid in the cytoplasm. bFGF-transfected VS cells, which spontaneously and efficiently proliferate while preserving their ability to differentiate into adipocytes, may be an adequate cell source for human adipose tissue regeneration.
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. yamasan@kuhp.kyoto-u.ac.jp
authors: Hiroyasu
authors: H
authors: Yamashiro
authors: Takashi
authors: T
authors: Inamoto
authors: Michio
authors: M
authors: Yagi
authors: Masaya
authors: M
authors: Ueno
authors: Hironori
authors: H
authors: Kato
authors: Megumi
authors: M
authors: Takeuchi
authors: Shin-Ichi
authors: S
authors: Miyatake
authors: Yasuhiko
authors: Y
authors: Tabata
authors: Yoshio
authors: Y
authors: Yamaoka
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D014162:Transfection
chemical_list: D016222:Fibroblast Growth Factor 2