[Bactericidal peptide targeted against penicillin/methicillin-resistant Staphylococcus aureus--an engineered multidomain protein machine].
OBJECTIVE
To construct a targeting bactericidal peptide machine by fusing two minidomains with different bioactivities and different protein origins.


METHODS
Such fusion peptide was constructed by linking the gene of Staphylococcal AgrD pheromone with the gene of C-terminal (I626) of colicin Ia pore-forming region (K544-I626) with site-directed mutation. Mutated plasmid was transformed into E. coli TG1 cells to produce fusion peptide, peptides were purified by CM sepharose ion-exchange column. In vitro bactericidal assays were made to identify the bioactivity of fusion peptide.


RESULTS
Fusion peptide presented a specific bactericidal activity which was over one hundred times as effective as that of penicillin/oxacillin against tested Staphylococcus aureus strains.


CONCLUSIONS
Fusion peptide behaved with a targeting bactericidal activity against Staphylococcus aureus which was lacking at two precursors, Staphylococcal pheromone and colicin Ia pore-forming region. These results suggest that an engineered multidomain protein machine with specific bactericidal activity has been constructed in the present study.
Liao
Ying
Y
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Jie
J
Lu
Xiaofeng
X
Wan
Lin
L
Li
Shengfu
S
Chen
Gang
G
Gao
Yang
Y
Qiu
Xiaoqing
X
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics*; D003087:Colicins / Q000235:genetics*; D005798:Genes, Bacterial; D016106:Methicillin Resistance / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D010403:Penicillin Resistance / Q000235:genetics; D010455:Peptides; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
C414532:AgrD protein, Staphylococcus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003087:Colicins; D010455:Peptides; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins