Gene expression profiling detects patterns of human macrophage responses following Mycobacterium tuberculosis infection.
High-density oligonucleotide microarrays allow simultaneous monitoring of the expression of a large number of cellular genes. Microarrays were used to screen the global human monocyte-derived macrophage transcriptional response to infection with the intracellular pathogen Mycobacterium tuberculosis. The microarray detected reproducible patterns of regulated gene expression. Analysis of the expression data showed induction of cytokines and chemokines, ribosomal proteins, and the interferon-response gene Stat1. Several changes were validated by quantitative reverse transcription polymerase chain reaction and immunoblot assays. Augmentation of the respiratory burst and preservation of the response to interferon-gamma were also demonstrated. These data supplement existing knowledge on macrophage responses to tuberculosis infection.
Wang
Jennifer P
JP
University of California, San Diego, Department of Medicine 0640, 9500 Gilman Drive, La Jolla, CA 92093-0640, USA.
Rought
Steffney E
SE
Corbeil
Jacques
J
Guiney
Donald G
DG
D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000378:metabolism; D019255:NADPH Oxidases / Q000032:analysis / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D016897:Respiratory Burst; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D018925:Chemokines; D016207:Cytokines; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D007371:Interferon-gamma; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1