[Effect of different tension stress on collagen synthesis in human periodontal ligaments fibroblasts in vitro].
OBJECTIVE
To observe the change in amount of collagen synthesis under different tension stress patterns in vitro.


METHODS
Human periodontal ligament fibroblasts (HPDLF) were harvested and subjected to the cellular mechanical research system for the detection of collagen synthesis under static and dynamic tension stress in different frequency and magnitude with 3H-Proline for 16 h, 24 h and 32 h.


RESULTS
Dynamic tension stress group 2(5 kPa-2.5 kPa-5 kPa) could most obviously increase collagen synthesis in HPDLF, and then control group (0 kPa), static tension stress group (5 kPa) and dynamic tension stress group 1(5 kPa-0 kPa-5 kPa) came next in order.


CONCLUSIONS
Tension stress pattern can affect collagen synthesis in HPDLF. Appropriate dynamic tension stress can increase the amount of collagen synthesis in HPDLF.
Zhao
Zhihe
Z
Department of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Zhou
Jixiang
J
Jiang
Lingyong
L
Lin
Zhu
Z
D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D013718:Tensile Strength
D003094:Collagen