title: [Study on the diagnostic significance of detecting the expression of AChR-gamma mRNA in rhabdomyosarcoma tissues].
abstract: OBJECTIVE
  To detect over-expression of AChR-gamma mRNA in rhabdomyosarcoma tissues by duplex RT-PCR and discuss its potential in diagnosis of rhabdomyosarcoma.
  
  
  METHODS
  Duplex RT-PCR was applied to the simultaneous detection of AChR-alpha and gamma subunit messenger RNA in 17 cases of rhabdomyosarcoma (9 ERMS, 6 ARMS, 2 PRMS). 20 cases of non-rhabdomyosarcomous small round cell tumors (6 poorly differentiated synovial sarcomas, 6 ES/PNET, 6 lymphomas, 2 neuroblastomas) and three normal muscle samples were also detected for AChR-alpha and gamma mRNA by the same method.
  
  
  RESULTS
  AChR-alpha and AChR-gamma mRNA were expressed in all the cases of rhabdomyosarcoma. The rate of quantity in both transcripts was AChR-gamma/AChR-alpha >or= 1, but the rate for three normal muscle samples was < 1. Cases of non-rhabdomyosarcomous small round cell tumors were all negative for AChR-gamma.
  
  
  CONCLUSIONS
  AChR-gamma mRNA expression detected by molecular genetic methods is useful in diagnosis and differential diagnosis of rhabdomyosarcoma.
authors: Department of Pathology, School of Medicine, Shihezi University, Shihezi 832002, China.
authors: Bin
authors: B
authors: Chang
authors: Feng
authors: F
authors: Li
authors: Li-juan
authors: LJ
authors: Pang
authors: Wen-hao
authors: WH
authors: Hu
authors: Tian-cai
authors: TC
authors: Lu
authors: Hong-an
authors: HA
authors: Li
authors: Jian
authors: J
authors: Wang
authors: Xiong-zeng
authors: XZ
authors: Zhu
authors: Da-ren
authors: DR
authors: Shi
mesh_terms: D003937:Diagnosis, Differential; D006801:Humans; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000235:genetics*; D011978:Receptors, Nicotinic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000175:diagnosis*
chemical_list: C539275:CHRNG protein, human; D021122:Protein Subunits; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic