title: Selective toxicity of engineered lentivirus lytic peptides in a CF airway cell model.
abstract: Lentivirus lytic peptides (LLPs) are derived from HIV-1 and have antibacterial properties. LLP derivatives (eLLPs) were engineered for greater potency against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA). Minimum bactericidal concentration (MBC) was determined in low and physiologic salt concentrations. MBC was decreased against SA and equivalent against PA in physiologic salt when compared to the parent compound LLP1. In a novel cystic fibrosis (CF) airway cell model, one derivative, WLSA5, reduced the number of adherent PA and only moderately affected CF cell viability. Overall, eLLPs are selectively toxic to bacteria and may be useful against CF airway infections.
authors: Division of Pediatric Pulmonology, The Children's Hospital of Pittsburgh, 3705 Fifth Avenue, 15213, Pittsburgh, PA, USA.
authors: Shruti M
authors: SM
authors: Phadke
authors: Kazi
authors: K
authors: Islam
authors: Berthony
authors: B
authors: Deslouches
authors: Sunil A
authors: SA
authors: Kapoor
authors: Donna
authors: D
authors: Beer Stolz
authors: Simon C
authors: SC
authors: Watkins
authors: Ronald C
authors: RC
authors: Montelaro
authors: Joseph M
authors: JM
authors: Pilewski
authors: Timothy A
authors: TA
authors: Mietzner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001980:Bronchi / Q000378:metabolism; D016956:Burkholderia cepacia / Q000187:drug effects; D003550:Cystic Fibrosis / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000494:pharmacology / Q000633:toxicity*; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology / Q000633:toxicity*; D015202:Protein Engineering; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; C116049:lentiviral lytic peptide type 1, Human immunodeficiency virus 1