title: Prevalence of infection and genotypes of GBV-C/HGV among homosexual men.
abstract: Since the discovery of GB virus-C (GBV-C) and hepatitis G virus (HGV), many studies have been performed. These viruses are now known to be parenterally, as well as sexually transmitted. A phylogenetic analysis also revealed that GBV-C has five major genotypes: type 1 predominates in West Africa, type 2 in Europe and the United States, type 3 in parts of Asia, type 4 in Southeast Asia, and type 5 in South Africa. Despite the number of reports so far, there have been few large-scale surveys of homosexual men to determine the prevalence of the GBV-C/HGV infections. We examined the levels of GBV-C/HGV viremia in 297 homosexual men who attended the Nagoya Lesbian and Gay Revolution held in Nagoya, Japan. Reverse transcription-polymerase chain reaction (RT-PCR)/nested PCR of the GBV-C/HGV 5 ' -non-coding region (NCR), and base sequence analyses showed that the infection rate was 12.5%, and genotypes in this population were classified into type 2 (32%) and type 3 (68%). None were classified as types 1, 4, or 5 in this study. Our results indicate that the GBV-C/HGV type 2 seen mainly in Europe and the US is spreading widely in Japan, especially in the Nagoya district.
authors: Clinical Research Center, Nagoya National Hospital (Tokai Area Central Hospital for AIDS Treatment and Research), Nagoya, aichi 460-0001, Japan.
authors: Junko
authors: J
authors: Hattori
authors: Shiro
authors: S
authors: Ibe
authors: Hiromi
authors: H
authors: Nagai
authors: Kaoru
authors: K
authors: Wada
authors: Takayuki
authors: T
authors: Morishita
authors: Katsuhiko
authors: K
authors: Sato
authors: Makoto
authors: M
authors: Utsumi
authors: Tsuguhiro
authors: T
authors: Kaneda
mesh_terms: D018178:Flaviviridae Infections / Q000453:epidemiology* / Q000821:virology; D029407:GB virus C / Q000145:classification / Q000235:genetics / Q000502:physiology; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000453:epidemiology* / Q000821:virology; D006716:Homosexuality; D006801:Humans; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA; D019562:Viral Load