Insights into the regulation of transcription by scanning force microscopy.
The scanning force microscope (SFM) is a valuable tool for the structural analysis of complexes between protein(s) and DNA. In recent years the application of scanning force microscopy to the field of transcription regulation has been reported in numerous studies. Using this technique, novel insights could be obtained into the architecture and dynamics of complexes, which are relevant to the transcription process and the mechanisms by which this process is regulated. In this article an overview is given of SFM studies addressing, in particular, topics in the field of transcription in prokaryotic organisms.
Dame
R T
RT
Physics of Complex Systems, Department of Physics and Astronomy, Free University of Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands. rtdame@nat.vu.nl
Wyman
C
C
Goosen
N
N
D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005786:Gene Expression Regulation; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D011387:Prokaryotic Cells / Q000648:ultrastructure*; D014158:Transcription, Genetic
D004247:DNA; D012321:DNA-Directed RNA Polymerases