[Identification of Aloe species by random amplified polymorphic DNA (RAPD) analysis].
Juice and integument of leaves of 3 Aloe species, Aloe vera, A. ferox and A. africana, are not allowed to be used as food according to the Pharmaceutical Affairs Law in Japan. On the other hand, whole leaves of A. arborescens can be used as food. The present study was designed to distinguish Aloe species by random amplified polymorphic DNA (RAPD) analysis. DNA was isolated from fresh and dried leaves of the 4 Aloe species. Five out of 32 different 10-mer primers examined were useful for analysis. By comparison of the characteristic bands of PCR products on agarose gel, it was possible to distinguish the 4 species. Thus, the botanical species of Aloe in commercial food products can be identified by RAPD analysis.
Shioda
Hiroko
H
Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Satoh
Kanako
K
Nagai
Fumiko
F
Okubo
Tomoko
T
Seto
Takako
T
Hamano
Tomoko
T
Kamimura
Hisashi
H
Kano
Itsu
I
D000504:Aloe / Q000145:classification* / Q000235:genetics; D018744:DNA, Plant / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D018744:DNA, Plant