cDNA cloning and expression analysis of the rice (Oryza sativa L.) RNase L inhibitor.
A subtractive cDNA library was constructed using rice (Oryza sativa L.) callus cDNA as driver and differentiating callus cDNA as tester. A novel cDNA fragment encoding RNase L inhibitor (RLI) was isolated by screening the subtractive library, which had a higher expression level in differentiating callus than in callus. The full-length cDNA of rice-RLI was obtained by the method of rapid amplification of cDNA ends, which contained a 1812-bp open reading frame encoding a 604 amino acid polypeptide. Homologous analysis showed that rice-RLI contained the conserved motifs (two repeated P-loops, two ATP-binding boxes and an iron-sulfur binding motif). The fluorescence quantitative PCR analysis showed that it was a constitutive expressed gene but up-regulated in abiotic stress (low temperature and NaCl treatment) and down-regulated under the treatments of NAA and IAA.
Du
Xi-Ling
XL
Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Wang
Dong
D
Qian
Xiao-Yin
XY
Jiang
Li-Zhi
LZ
Chun
Wei
W
Li
Ke-Gui
KG
Shen
Guo-An
GA
Lin
Chang-Fa
CF
Yang
Jin-Shui
JS
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D018833:Chaperonins / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005453:Fluorescence; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature; D013997:Time Factors
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D018076:DNA, Complementary; D012965:Sodium Chloride; D018833:Chaperonins