Mercury contamination of rat amylin mimics vasoactivity and cytotoxic effects.
Rat amylin differs from human amylin (hIAPP) in that it lacks a fibril-forming capacity. As a consequence, toxic effects have been reported for human but not for rat amylin. This report demonstrates how a mercury contamination of commercial rat amylin imitates peptide-related vasoactive and cytotoxic effects on preparations of isolated blood vessels. The source of mercury contamination was believed related to the peptide synthesis. Thiol groups of cysteine-containing peptides are often protected by acetamidomethyl (Acm) which is cleaved by mercuric acetate.
Golpon
Heiko A
HA
Department of Internal Medicine, Philipps University of Marburg, Baldinger Strasse, 35033, Marburg, Germany. Heiko.Golpon@medizin.uni-magdeburg.de
Püchner
Annette
A
Schmidt
Lothar
L
Jungclas
Hartmut
H
Barth
Peter
P
Welte
Tobias
T
Wichert
Peter V
PV
Feddersen
Christian O
CO
D000109:Acetylcholine / Q000378:metabolism; D000682:Amyloid / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D002142:Californium / Q000378:metabolism; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008297:Male; D013058:Mass Spectrometry; D008628:Mercury / Q000378:metabolism* / Q000633:toxicity; D011651:Pulmonary Artery / Q000187:drug effects*; D051381:Rats
D000682:Amyloid; D000897:Anti-Ulcer Agents; D058228:Islet Amyloid Polypeptide; D002142:Californium; D008628:Mercury; D000109:Acetylcholine