Development of mitochondrial SSU rDNA-based oligonucleotide probes for specific detection of common airborne fungi.
In this study we sequenced partial mitochondrial small subunit rDNA from 32 fungal strains representing 31 species from 16 genera. Most of these species are common airborne fungi and pathogens that may cause various public health concerns. Sequence alignment showed several conserved and highly variable regions. The variable regions were deployed to design oligonucleotide probes for each fungal species. The specificity of the designed probes was first examined through homology search against GenBank database then further verified through hybridization experiments to 38 fungal strains. A total of 23 probes were verified as specific to 15 fungal species commonly detected in living and working environments. These new probes will have potential applications in clinical diagnosis and public health-related environmental monitoring.
Zeng
Qing-Yin
QY
National Institute for Working Life, SE-90713 Umeå, Sweden.
Wang
Xiao-Ru
XR
Blomquist
Göran
G
D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005658:Fungi; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics*; D017421:Sequence Analysis
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes