Allatostatin in ovaries, oviducts, and young embryos in the cockroach Diploptera punctata.
The quantity and localization of -Phe-Gly-Leu-amide allatostatins (-F-G-L-amide AST) was determined by ELISA and immunohistochemistry in ovaries and oviducts and in pre-dorsal closure embryos. AST in the cytoplasm of basal oocytes gradually increased from 4 to 35 fmol/ovary pair from the start (day 2) to the completion of vitellogenesis (day 6), then rapidly increased to 121 fmol/ovary pair during choriogenesis. In oviducts, AST-immunoreactivity was found in nerves to the muscle layer and in epithelial cells. AST-immunoreactivity in oviduct epithelial cells increased during vitellogenesis. A marked increase in quantity of AST in oviduct tissue between completion of chorion formation and immediately after ovulation appears to result from AST released from oocytes as they travel down the oviducts because AST content of newly ovulated eggs was 40% lower than late stage chorionated oocytes, and these oocytes released AST when incubated in saline. AST in embryos, localized in yolk cells, decreased as embryos approached dorsal closure. That this material in ovaries and embryos is AST was confirmed by its ability to inhibit JH synthesis in vitro and identification by MALDI-TOF mass spectrometry of a peptide with a mass corresponding to that of a Diploptera punctata AST. These findings indicate likely novel functions for ASTs: facilitation of ovulation and utilization of yolk.
Woodhead
A P
AP
Department of Biological Sciences, University of Iowa, Biology Building, Iowa City, IA 52242, USA.
Thompson
M E
ME
Chan
K K
KK
Stay
B
B
D000818:Animals; D003058:Cockroaches / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D010057:Oviducts / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D010060:Ovulation / Q000378:metabolism; D014818:Vitellogenesis / Q000502:physiology
D009479:Neuropeptides; C076523:allatostatin