title: Age-associated changes in functional response to CXCR3 and CXCR5 chemokine receptors in human osteoblasts.
abstract: The expression and functional activity of CXC chemokine receptors were evaluated in human osteoblasts (OB) obtained post-trauma from old donors compared to very young donors. It was found that CXCR1 and CXCR4 were only expressed by old but not young donors' cells. In contrast, CXCR3 and CXCR5 were expressed by both young and old donors. We functionally evaluated CXCR3/CXCL10 and CXCR5/CXCL13 receptor/ligand pairs by analysing cell proliferation and the release of N-acetyl-beta-D-glucosaminidase (NAG), an enzyme that degrades glycosaminoglycans and hyaluronic acid. CXCL10 and CXCL13 induced a dose-dependent increase of cell proliferation in OB from young donors while cell proliferation of OB in old donors was not affected. By contrast, CXCL10 and CXCL13 induced a significantly higher NAG release in OB from old donors compared to young ones. These data demonstrate a significant age-dependent difference in the response of OB to CXCL10 and CXCL13 stimulation. These chemokines induce an inverse response of OB from old and young donors, which suggests a role of ageing in the modulation of cellular response of bone cells.
authors: Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy. labimge@alma.unibo.it
authors: Gina
authors: G
authors: Lisignoli
authors: Anna
authors: A
authors: Piacentini
authors: Stefania
authors: S
authors: Toneguzzi
authors: Francesco
authors: F
authors: Grassi
authors: Matilde
authors: M
authors: Tschon
authors: Sandra
authors: S
authors: Cristino
authors: Andrea
authors: A
authors: Facchini
authors: Erminia
authors: E
authors: Mariani
mesh_terms: D000368:Aged; D000375:Aging / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000378:metabolism / Q000494:pharmacology; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D054367:Receptors, CXCR3; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine / Q000378:metabolism*; D018121:Receptors, Cytokine / Q000378:metabolism*
chemical_list: C114753:CXCL10 protein, human; C110663:CXCL13 protein, human; C515187:CXCR3 protein, human; C095085:CXCR5 protein, human; D054357:Chemokine CXCL10; D054382:Chemokine CXCL13; D019743:Chemokines, CXC; D054367:Receptors, CXCR3; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D000469:Alkaline Phosphatase