[Establishment and identification of recombinant NS-1 cell strain that stably expresses chimeric HBc containing HBV multiepitope short peptides].
OBJECTIVE
To establish recombinant NS-1 cell strain that is capable of stable expression of chimeric HBc particle containing HBV multi epitope short peptides.


METHODS
The recombinant plasmid, pHBc-Mep, was transfected into NS-1 cells via Lipofectamine, and the recombinant cell strain was screened with G418 and subclone screening. The expression products of the cells were examined by RT-PCR, ELISA, indirect immunofluorescence assay (IFA) and Western blotting.


RESULTS
The results of RT-PCR, ELISA, IFA and Western blotting demonstrated that the recombinant protein HBc-Mep was expressed in the screened cells after continuous cloning for 3 times, but not in cells transfected with pcDNA3.1 or nontransfected cells.


CONCLUSIONS
The recombinant cell strain stably expressing chimeric HBc particle containing multi epitope short peptides of HBV, designated as NS/HBc-Mep, has been established successfully.
Tian
Ze-wei
ZW
Research Center for Hepatopathy of PLA, 458 Hospital of PLA, Guangzhou 510602, China.
Dong
Wen-qi
WQ
Liu
Zhao-xia
ZX
D000818:Animals; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006512:Hepatitis B Core Antigens / Q000235:genetics* / Q000276:immunology; D051379:Mice; D010957:Plasmids; D036261:Receptor, EphB6; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D006512:Hepatitis B Core Antigens; D036261:Receptor, EphB6; D011993:Recombinant Fusion Proteins