title: X-ray structure of glutathione S-transferase from the malarial parasite Plasmodium falciparum.
abstract: GSTs catalyze the conjugation of glutathione with a wide variety of hydrophobic compounds, generally resulting in nontoxic products that can be readily eliminated. In contrast to many other organisms, the malarial parasite Plasmodium falciparum possesses only one GST isoenzyme (PfGST). This GST is highly abundant in the parasite, its activity was found to be increased in chloroquine-resistant cells, and it has been shown to act as a ligandin for parasitotoxic hemin. Thus, the enzyme represents a promising target for antimalarial drug development. We now have solved the crystal structure of PfGST at a resolution of 1.9 A. The homodimeric protein of 26 kDa per subunit represents a GST form that cannot be assigned to any of the known GST classes. In comparison to other GSTs, and, in particular, to the human isoforms, PfGST possesses a shorter C-terminal section resulting in a more solvent-accessible binding site for the hydrophobic and amphiphilic substrates. The structure furthermore reveals features in this region that could be exploited for the design of specific PfGST inhibitors.
authors: Department of Biophysics, Max Planck Institute for Medical Research, D-69120 Heidelberg, Germany. fritz@mpimf-heidelberg.mpg.de
authors: Karin
authors: K
authors: Fritz-Wolf
authors: Andreas
authors: A
authors: Becker
authors: Stefan
authors: S
authors: Rahlfs
authors: Petra
authors: P
authors: Harwaldt
authors: R Heiner
authors: RH
authors: Schirmer
authors: Wolfgang
authors: W
authors: Kabsch
authors: Katja
authors: K
authors: Becker
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D019281:Dimerization; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000962:Antimalarials; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D005982:Glutathione Transferase