A recombinant fragment of human SP-D reduces allergic responses in mice sensitized to house dust mite allergens.
C57Bl6 mice sensitized to Dermatophagoides pteronyssinus and challenged with D. pteronyssinus allergen extract given intranasally followed by treatment with intranasal applications of a 60-kDa truncated, trimeric recombinant form of human SP-D (rfhSP-D) showed a significant reduction in serum IgE, IgG1, peripheral blood eosinophilia and airway hyperresponsiveness compared to saline or bovine serum albumin-treated controls. Intracellular cytokine staining of lung and spleen homogenates showed increases in interleukin (IL)-12 production in lung tissue and normalization of IL-12 and interferon (IFN)-gamma in spleen tissue. In previous studies we demonstrated the effectiveness of native SP-D and rfhSP-D in down-regulating allergic responses to allergens of Aspergillus fumigatus. The results reported here indicate that rfhSP-D can suppress the development of allergic symptoms in sensitized mice challenged with allergens of the common house dust mite.
Strong
P
P
Medical Research Council Immunochemistry Unit, Department of Biochemistry, University of Oxford, UK.
Townsend
P
P
Mackay
R
R
Reid
K B M
KB
Clark
H W
HW
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004802:Eosinophilia / Q000517:prevention & control; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000276:immunology; D037663:Pulmonary Surfactant-Associated Protein D / Q000276:immunology*; D011663:Pulmonary Surfactants / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013154:Spleen / Q000276:immunology
D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D010446:Peptide Fragments; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D018664:Interleukin-12; D007073:Immunoglobulin E; D007371:Interferon-gamma