Variations in the ability of adsorbed fibrinogen to mediate platelet adhesion to polystyrene-based materials: a multivariate statistical analysis of antibody binding to the platelet binding sites of fibrinogen.
Platelet adhesion to the surfaces of biomaterials preadsorbed with plasma previously has been shown to be mediated exclusively by surface-bound fibrinogen and does not seem to involve the other adhesion proteins in plasma (Tsai et al., J Biomed Mater Res 2002;60:348-359). In this study, the influence of surface-bound fibrinogen on platelet adhesion to five different types of polystyrene-based microtiter plates preadsorbed with plasma was analyzed relative to the amount of adsorbed fibrinogen and monoclonal antibody binding to the adsorbed fibrinogen. There was no significant correlation between platelet adhesion and the absolute amount of adsorbed fibrinogen. However, platelet adhesion was positively correlated to the ability of the adsorbed fibrinogen to bind three types of monoclonal antibodies. The antibodies used bound to the sites on fibrinogen thought to be involved in platelet binding (the two gamma chain C-terminal dodecapeptides and the RGDF and RGDS sequences in each of the Aalpha chains). A partial least-squares calibration model was used to analyze the relative importance of these binding sites in fibrinogen to platelet adhesion. The gamma chain C-terminal dodecapeptide was shown to be the most important site in adsorbed fibrinogen in mediating platelet adhesion.
Tsai
Wei-Bor
WB
Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195, USA.
Grunkemeier
John M
JM
Horbett
Thomas A
TA
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism*; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D015999:Multivariate Analysis; D010455:Peptides / Q000378:metabolism; D010949:Plasma / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000502:physiology*; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013223:Statistics as Topic; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D010455:Peptides; D011137:Polystyrenes; D005340:Fibrinogen