title: Characterization of the human polymeric immunoglobulin receptor (PIGR) 3'UTR and differential expression of PIGR mRNA during colon tumorigenesis.
abstract: The human cell lines VACO-235 and VACO-411 constitute a novel in vitro model of colon adenoma to carcinoma progression. By differential display RT-PCR we identified a transcript that is expressed in the parental nontumorigenic adenoma line (VACO-235E), but is not expressed in the tumorigenic daughter (VACO-235L) or granddaughter (VACO-411) lines. This cDNA represents a previously uncharacterized portion of the 3'UTR of human PIGR. Human PIGR mRNA was found to be highly expressed in normal colon epithelium, but was decreased in 6 of 8 colon tumors and was negligible in 8 of 10 colon tumor cell lines. We sequenced the entire 1.8 kb 3'UTR of human PIGR, and found it to contain multiple repetitive elements as well as elements that could affect the processing and stability of PIGR mRNA. We hypothesize that differential regulation of PIGR mRNA stability may contribute to its downregulation in colon cancer.
authors: Department of Molecular Biology and Microbiology, Case Western Reserve University, University Hospitals of Cleveland, Cleveland, Ohio, USA. jtraicoff@2020gene.com
authors: June L
authors: JL
authors: Traicoff
authors: Laura
authors: L
authors: De Marchis
authors: Britten L
authors: BL
authors: Ginsburg
authors: Rodolfo E
authors: RE
authors: Zamora
authors: Nada H
authors: NH
authors: Khattar
authors: Vincent J
authors: VJ
authors: Blanch
authors: Sarah
authors: S
authors: Plummer
authors: Sharon A
authors: SA
authors: Bargo
authors: Dennis J
authors: DJ
authors: Templeton
authors: Graham
authors: G
authors: Casey
authors: Charlotte S
authors: CS
authors: Kaetzel
mesh_terms: D000236:Adenoma / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002277:Carcinoma / Q000235:genetics*; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D003110:Colonic Neoplasms / Q000235:genetics*; D017931:DNA Primers; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D019056:Receptors, Polymeric Immunoglobulin