Tolerance limit of rats to normothermic hepatic inflow occlusion under portal blood bypass.
OBJECTIVE
To evaluate the tolerance limit of rats to normothermic hepatic inflow occlusion under portal blood bypass.


METHODS
A new rat model of normothermic hepatic inflow occlusion under portal blood bypass was established by clamping temporarily the pedicles of all liver lobes while the caudal lobe was kept as a passage of the portal blood flow. After hepatic blood flow restored, the caudal lobe was cut off. On the 7th postoperative day, survival rate, hepatic morphological changes, and the severity and reversibility of the injured energy metabolism of the liver were investigated.


RESULTS
All rats that had been subjected to 30, 60 and 90 minutes of hepatic inflow occlusion under portal blood bypass survived on the 7th postoperative day. ischemia-reperfusion injury of the liver was reversible and compensatory in rats with hepatic inflow occlusion within 90 minutes. However, the survival rates of rats with 100, 110 and 120 minutes of hepatic inflow occlusion were 50%, 30% and 20% respectively. Liver injury of rats with 120 minutes of hepatic inflow occlusion was severe and Irreversible.


CONCLUSIONS
The tolerance limit of rats to normothermic hepatic inflow occlusion is enhanced significantly under portal blood bypass and the upper limit is 90 minutes.
Dong
Jia-Hong
JH
Hepatobiliary Surgery Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
He
Xiao-Dong
XD
Li
Kun
K
Duan
Heng-Chun
HC
Peng
Zhi-Ming
ZM
Cai
Jing-Xiu
JX
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D008099:Liver / Q000473:pathology / Q000502:physiology* / Q000601:surgery*; D008297:Male; D008928:Mitochondria / Q000502:physiology; D011168:Portal System / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D015427:Reperfusion Injury / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D015996:Survival Rate; D013696:Temperature; D013997:Time Factors
D000255:Adenosine Triphosphate