Acetaldehyde induces granulocyte macrophage colony-stimulating factor production in human bronchi through activation of nuclear factor-kappa B.
Acetaldehyde, a metabolite of alcohol and primary mediator of alcohol-induced asthma, causes bronchoconstriction via histamine release from airway mast cells. Acetaldehyde also is found in cigarette smoke and may cause airway inflammation. The purpose of this study was to determine the effect of acetaldehyde on cytokine production and nuclear factor kappa B (NF-kappa B) activation in human bronchial tissues. Human bronchi were prepared from normal parts of lung tissues resected for lung cancer (n = 11). The bronchi were cultured in the presence of 5 x 10(-4) M of acetaldehyde for 24 hours and the concentrations of eotaxin, granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-5, interleukin-8, and regulated on activation, normal T cells expressed and secreted in cultured supernatants were determined by enzyme-linked immunosorbent assay. Tissues also were immunohistochemically stained for NF-kappa Bp65. Acetaldehyde significantly increased GM-CSF production from human bronchi and nuclear translocation of NF-kappa Bp65 in airway epithelium but had no effects on other cytokines. Our findings suggest that acetaldehyde potentially causes airway inflammation via increased GM-CSF production through nuclear translocation of NF-kappa B.
Machida
Ikuko
I
Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Matsuse
Hiroto
H
Kondo
Yuki
Y
Kawano
Tetsuya
T
Saeki
Sachiko
S
Tomari
Shinya
S
Fukushima
Chizu
C
Shimoda
Terufumi
T
Kohno
Shigeru
S
D000079:Acetaldehyde / Q000494:pharmacology*; D000368:Aged; D000369:Aged, 80 and over; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism*; D001991:Bronchitis / Q000139:chemically induced / Q000378:metabolism*; D046508:Culture Techniques; D016207:Cytokines / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*
D016207:Cytokines; D016328:NF-kappa B; D006632:Histamine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000079:Acetaldehyde