[The expression and clinical implication of gelatinase A in acute leukemic cells].
OBJECTIVE
To study the expression of gelatinase A (MMP2) in acute leukemic cells and its clinical implication.


METHODS
The bone marrow samples from 46 acute leukemia patients were investigated by reverse transcription polymerase chain reaction (RT-PCR) and Gelatin Zymography method. Matrigel invasion assay was studied on U937, NB4, K562 and SHI1 cells in vitro.


RESULTS
The expression of MMP2 was positive in 24 of 46 patients with AL (52.2%). In MMP2 positive and negative groups of AL, the extramedullary infiltration rates were 50.0% and 18.2% (P < 0.05), whilst the percentage of blast cell in peripheral white blood cells were (77.21 +/- 13.9)% and (62.95 +/- 17.2)% (P < 0.01), respectively. The positive expression of TIMP2 and MT1-MMP in 46 AL patients were 27 (58.7%) and 33 (71.7)%. The matrigel invasion assay suggested that MMP2 is involved in migration of leukemic cell through extracellular matrix (Matrigel).


CONCLUSIONS
The active form of MMP2 might be essential to the egress of leukemic cells from bone marrow into peripheral blood, followed by an extramedullary infiltration.
Li
Sheng
S
Department of Hematology, The First Hospital Affiliated to Soochow University, Suzhou 215006, China.
Chen
Zi-xing
ZX
Wang
Wei
W
Cen
Jian-nong
JN
Fu
Jian-xin
JX
Yao
Li
L
D000208:Acute Disease; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D017254:Leukemic Infiltration; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000502:physiology*; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D020778:Matrix Metalloproteinase 2