title: Renal and vascular nitric oxide system in reduced renal mass saline hypertension.
abstract: BACKGROUND
  Reduction in renal mass is associated with several structural and functional adaptations including compensatory renal growth and hemodynamic changes. The mediators of the renal hemodynamic adaptations have not been definitively identified. Several investigators have postulated that nitric oxide (NO) is involved this physiological mechanisms. The purpose of this study was to evaluate the role of vascular and renal NO pathway in the model of subtotal nephrectomy-salt load hypertension.
  
  
  METHODS
  Wistar rats with 75% renal mass reduction (RMR) and saline load were studied during 4 weeks. Weekly, indirect systolic blood pressure (SBP) were measured. One week after nephrectomy, animals were divided in two groups, hypertensive (SBP > 140 mm Hg) and normotensive (SBP < 140 mm Hg). Urinary excretion of nitrates and nitrites (NOx), urinary chemioluminiscence levels and NOS activity in the left kidney and in the thoracic aorta artery were determined at the fourth week after subtotal nephrectomy.
  
  
  RESULTS
  Urinary excretion of sodium was higher in normotensive rats than hypertensive rats and in both groups this parameter was higher than in sham rats. NOx excretion and NOS activity in the different nephron segments were higher in normotensive rats than in the hypertensive ones. In contrast, NOS activity in aorta sections and urinary chemiluminescence levels in hypertensive animals were enhanced compared with normotensive rats. These parameters were higher in both groups of nephrectomized rats than in sham ones.
  
  
  CONCLUSIONS
  This study provides evidence to support the fact that the activation of the renal NO system is an important mechanism whereby the remnant kidney regulates sodium and water balance, contributing to control the arterial blood pressure in the renal mass reduction and saline load model.
authors: Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina. carranz@huemul.ffyb.uba.ar
authors: Cristina
authors: C
authors: Arranz
authors: Analía
authors: A
authors: Tomat
authors: Andrea
authors: A
authors: Fellet
authors: Julia
authors: J
authors: García
authors: Ana María
authors: AM
authors: Balaszczuk
authors: María
authors: M
authors: de los Angeles Costa
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D004195:Disease Models, Animal; D005919:Glomerular Filtration Rate / Q000502:physiology; D006977:Hypertension, Renal / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology*; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology* / Q000601:surgery; D008297:Male; D009392:Nephrectomy / Q000379:methods; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012462:Saline Solution, Hypertonic / Q000009:adverse effects
chemical_list: D012462:Saline Solution, Hypertonic; D009569:Nitric Oxide