Relationship between genetic polymorphism of cytochrome P450IIE1 and fatty liver.
OBJECTIVE
To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.


METHODS
Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients without fatty liver and 20 normal controls. Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst I and Rsa I.


RESULTS
The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6%), obese fatty liver (38.5%), or diabetic fatty liver (33.3%) was significantly lower than that of the corresponding patients without fatty liver (100%, 100% and 80% respectively), while the frequency of C2 genes, including C1/C2 and C2/C2, was significantly higher (71.4%/0%, 61.5%/0%, and 66.7%/20%) (P<0.01). The frequency distribution of the above genes of non-fatty liver patients (100%/0, 100%/0, and 80%/20%) was not significantly different from that of the normal controls (85%/15%) (P>0.05).


CONCLUSIONS
The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver. Besides, C2 gene may play a key role in the pathogenesis of fatty liver.
Piao
Yun-Feng
YF
Department of Gastroenterology, First Hospital, Jilin University, Changchun 130021, Jilin Province, China.
Li
Jing-Tao
JT
Shi
Yang
Y
D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005235:Fatty Liver, Alcoholic / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length
D019392:Cytochrome P-450 CYP2E1