Wogonin suppresses cellular proliferation and expression of monocyte chemoattractant protein 1 in Peyronie's plaque-derived cells.
OBJECTIVE
To test the effect of wogonin on cellular proliferation and expression of monocyte chemoattractant protein 1 (MCP-1) in cells derived from normal and diseased tunica albuginea (TA), as related to Peyronie's disease (PD).


METHODS
Cells with characteristics of fibroblasts were isolated from three tissue sources. Those from the plaque of patients with PD were designated as P cells, those from the adjacent, normal-appearing tissue as C cells, and those from the TA of patients without PD as N cells. These cells were treated with wogonin at doses of 0, 10, 20 and 40 micromol/L for 24 h or treated at a fixed dose of 40 micromol/L for 1, 8 and 24 h. Cell proliferation was assayed with a commercial kit, MCP-1 mRNA expression by reverse transcription-polymerase chain reaction, and secreted MCP-1 by enzyme-linked immunosorbent assay.


RESULTS
Wogonin suppressed cell proliferation in a dose-dependent manner; the effect was more pronounced against P cells at 8 and 24 h. Wogonin down-regulated MCP-1 mRNA expression, especially in P cells. Wogonin suppressed the level of secreted MCP-1 by 59-88%. P cells, which secreted far more MCP-1 than N and C cells at 1 h, were suppressed by 88%. C cells were the least suppressed at all three times.


CONCLUSIONS
Wogonin suppressed the proliferation, the expression of MCP-1 mRNA, and the expression of secreted MCP-1 in TA-derived cells. In most cases, the effect of wogonin was greatest against cells derived from the plaque. Wogonin appears to be a worthy candidate for preclinical trials in men with PD.
Wang
Z
Z
Department of Urology, School of Medicine, University of California, San Francisco, USA.
Lin
G
G
Lue
T F
TF
Lin
C S
CS
D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D002455:Cell Division; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D044950:Flavanones / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D010411:Penile Induration / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010413:Penis / Q000187:drug effects / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D012313:RNA; C085514:wogonin