Stability of natamycin and its cyclodextrin inclusion complexes in aqueous solution.
Aqueous solutions of natamycin and its beta-cyclodextrin (beta-CD), hydroxypropyl beta-cyclodextrin, and gamma-cyclodextrin (gamma-CD) inclusion complexes were completely degraded after 24 h of exposure to 1000 lx fluorescent lighting at 4 degrees C. After 14 days of storage in darkness at 4 degrees C, 92.2% of natamycin remained in active form. The natamycin:beta-CD complex and natamycin:gamma-CD complex were significantly more stable (p < 0.05) than natamycin in its free state in aqueous solutions stored in darkness at 4 degrees C. Clear poly(ethylene terephthalate) packaging with a UV light absorber allowed 85.0% of natamycin to remain after 14 days of storage under 1000 lx fluorescent lighting at 4 degrees C. Natamycin:cyclodextrin complexes can be dissociated for analysis in methanol/water/acetic acid, 60:40:5, v/v/v. Natamycin and its complexes in dissociated form were quantified by reverse phase HPLC with detection by photodiode array at 304 nm.
Koontz
John L
JL
Department of Food Science and Technology, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
Marcy
Joseph E
JE
Barbeau
William E
WE
Duncan
Susan E
SE
D000935:Antifungal Agents / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004355:Drug Stability; D008027:Light; D000432:Methanol; D010866:Natamycin / Q000737:chemistry*; D012996:Solutions; D014867:Water; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D000935:Antifungal Agents; D003505:Cyclodextrins; D012996:Solutions; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D014867:Water; D010866:Natamycin; C031215:betadex; C023792:gamma-cyclodextrin; D000432:Methanol