The effect of nadroparine on coagulation mechanisms: ultrastructural analysis.
Low molecular weight heparins are widely used in the prophylaxis and treatment of thrombotic disorders. The effect of low molecular weight heparins on coagulation was examined ultrastructurally in an animal model. A test and a control group was formed, each consisting of five rabbits. Nadroparine (225 Institute of Chaoy Unit/kg twice daily) was applied to the test group for 10 days. The control group received 1 ml saline solution subcutaneously. Blood and vascular tissue samples collected at the end of the 10th day were evaluated under a JEM 100 B electron microscope. Platelet degranulation and agglutination was observed in the control group. Fibrin materials were detected in the cytoplasms and surroundings of degranulated platelets. Erythrocyte accumulation was remarkable on the vascular endothelium with intact coagulation periods. In the test group, outer membranes of platelets, hyalomere, and granular structures in the granulomeres were detected to be nearly intact. There were rare erythrocytes in the large vascular lumens. The aggregation phase had occurred but no agglutination was detected. Nadroparine seems to preserve consistency of lipoprotein membranes of platelets and granular structures containing enzymes, which contribute to the coagulation mechanisms.
Sunar
Hasan
H
Department of Cardiovascular Surgery and Department of Pathology, Trakya University, Medical Faculty, Edirne, Turkey. hasansunar@yahoo.com
Hüseyinova
Gülara
G
Canbaz
Suat
S
Halici
Umit
U
Duran
Enver
E
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D005337:Fibrin / Q000187:drug effects / Q000648:ultrastructure; D006487:Hemostasis / Q000502:physiology; D017762:Nadroparin / Q000494:pharmacology*; D011817:Rabbits
D000925:Anticoagulants; D017762:Nadroparin; D005337:Fibrin