title: Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface.
abstract: With the help of a microfabrication process and surface modification technology, a method of fabricating protein patterned chips was developed which can be utilized as a powerful tool for performing bioassays in a high-throughput manner. A digital micromirror array (MMA) system was used as a virtual photomask, so that a maskless photolithography process was able to be used to build patterned biomolecules on a chip by selective illumination onto the chip surface. We utilized the nitroveratryloxycarbonyl (NVOC) group as a photolabile protecting group for protein patterning. The NVOC-protected surface was selectively irradiated by a UV illuminator using an MMA. After removing the NVOC group, biotin was coupled to the NVOC-cleaved site, onto which a buffered streptavidin solution was eluted. At this point, we could obtain a streptavidin-patterned surface and observe the effect of the polymer-grafted surface in reducing nonspecific binding.
authors: School of Chemical Engineering, Seoul National University, Kwanak-Gu, Seoul 151-742, South Korea.
authors: Dong-Sik
authors: DS
authors: Shin
authors: Kook-Nyung
authors: KN
authors: Lee
authors: Ki-Hoon
authors: KH
authors: Jang
authors: Jae-Kwon
authors: JK
authors: Kim
authors: Woo-Jae
authors: WJ
authors: Chung
authors: Yong-Kweon
authors: YK
authors: Kim
authors: Yoon-Sik
authors: YS
authors: Lee
mesh_terms: D000327:Adsorption; D001710:Biotin / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D005898:Glass; D010781:Photography / Q000379:methods; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D014867:Water; D001710:Biotin; D019809:Streptavidin