Transfusion-transmitted virus co-infection in other types of hepatitis and its genotypes.
OBJECTIVE
To identify the influence of transfusion transmitted virus (TTV) co-infection in other virus infected patients and its genotypes.


METHODS
A conservative sequence of ORF1 in the TTV genome was selected as primers and TTV DNA was measured in students and other hepatitis patients by using microplate nucleic acid hybridization and ELISA. The results were statistically analyzed. Nucleotide sequence of divergence>50% was used as color probe for distinguishing TTV genotypes I or II.


RESULTS
TTV DNA was detected in the sera from 2 (3.3%) of 60 students, 2 (14.3%) of 14 patients with non A-non E hepatitis, 6 (12%) of 50 patients with chronic hepatitis B, and 4 (16%) of 25 patients with liver cirrhosis, respectively. Statistical difference was observed between the patient group and the student group (P<0.05), but no significant difference in age, gender, serum ALT levels and TBiL between TTV DNA positive and negative patients (P>0.05). TTV genotype type I was by far the most frequent viral genotype (66.7%), followed by type II (25%), and mixed infection (8.3%).


CONCLUSIONS
These results suggest that the routes of TTV infection may be similar to those of HBV and HCV, and concurrent infection with HBV, HCV are common. TTV co-infection could not affect the clinical features of patients with liver diseases and the pathological process. TTV is not a main causative factor for patients with non A-non E hepatitis. Further study is needed to clarify the role of TTV in patients with non A-non E hepatitis.
Zhao
Shou-Song
SS
Department of Infectious Diseases, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Zhao
Jiu-Fa
JF
Gao
Chun-Ming
CM
Wang
Shu-Min
SM
D004266:DNA Virus Infections / Q000150:complications* / Q000821:virology; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000150:complications*; D006801:Humans; D022783:Torque teno virus / Q000235:genetics* / Q000302:isolation & purification