title: DNA typing: an important step forward? Collaborative Transplant Study.
abstract: In a collaborative project which was supported by 96 transplant centers, DNA typing of HLA-DR antigens was carried out on over 7,000 transplant donors and recipients at 8 participating laboratories. Approximately 25% of the individuals were found to have been typed incorrectly by serological means. An analysis of over 2,500 first cadaver kidney transplants showed a significant correlation of matching for the HLA-DR antigens in transplants where the serological typing was confirmed by DNA typing. In transplants where the serological typing was found to be incorrect, the analysis of serological HLA-DR mismatches resulted in no correlation with graft outcome whereas a significant correlation was found when the corrected DNA typed HLA-DR antigens were analyzed. Transplants which had been reported to the Collaborative Transplant Study based on serological typing as matched for HLA-A, -B, -DR or HLA-B, -DR were found to have a superior graft survival rate only if HLA-DR compatibility was confirmed by DNA typing.
authors: Transplant Immunology Laboratory, University of Heidelberg, Germany.
authors: G
authors: G
authors: Opelz
authors: J
authors: J
authors: Mytilineos
authors: S
authors: S
authors: Scherer
authors: H
authors: H
authors: Dunckley
authors: J
authors: J
authors: Trejaut
authors: J
authors: J
authors: Chapman
authors: D
authors: D
authors: Middleton
authors: D
authors: D
authors: Savage
authors: G
authors: G
authors: Fischer
authors: J D
authors: JD
authors: Bignon
authors: J C
authors: JC
authors: Bensa
authors: E
authors: E
authors: Albert
authors: H
authors: H
authors: Noreen
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D006085:Graft Survival / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D014181:Transplantation Immunology
chemical_list: D006684:HLA-DR Antigens