Impaired expression of acyl-CoA-synthetase 5 in epithelial tumors of the small intestine.
Fatty acids are implicated in tumorigenesis, but data are limited concerning endogenous fatty acid metabolism of tumor cells in adenomas and adenocarcinomas of the small intestine. The recently cloned human acyl-CoA-synthetase 5 (ACS5) is predominantly found in the small intestine and represents a key enzyme in providing cytosolic acyl-CoA thioesters. Protein synthesis and mRNA expression of ACS5 were studied in human intestinal tissues using different methods, including a newly established monoclonal antibody. In the healthy small intestine, expression of ACS5 was restricted to the villus surface epithelium but was not detectable in enterocytes lining crypts. ACS5 protein and mRNA were progressively diminished in epithelial cells of adenomas and adenocarcinomas of the small intestine. In conclusion, altered expression of ACS5 is probably related to the adenoma-carcinoma sequence of small intestinal epithelial tumors due to an impaired acyl-CoA thioester synthesis.
Gassler
Nikolaus
N
Department of Pathology, University of Heidelberg, Germany.
Schneider
Armin
A
Kopitz
Jürgen
J
Schnölzer
Martina
M
Obermüller
Nicholas
N
Kartenbeck
Jürgen
J
Otto
Herwart F
HF
Autschbach
Frank
F
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000236:Adenoma / Q000201:enzymology* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D003066:Coenzyme A Ligases / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000201:enzymology / Q000473:pathology; D007414:Intestinal Neoplasms / Q000201:enzymology* / Q000473:pathology; D007421:Intestine, Small / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D003066:Coenzyme A Ligases; C115795:acyl CoA synthetase 5; C485465:ACSL5 protein, human