Scanning electron microscopy evaluation of the link between a new dentinal desensitizer and dentine.
OBJECTIVE
The purpose of this study was to evaluate the interaction of a novel dentinal desensitizer Twin Pro with enamel-dentinal adhesive preparations or filling materials and dentine using scanning electron microscopy (SEM) technology.


METHODS
Black's I class cavities were drilled extracted molar teeth free of caries or fissures, and the cavities disinfected. The specimens were divided into 4 groups of 2 teeth each treated as follows: Group A: Twin Pro, fluid (Tetric-Flow Vivadent) and micro-hybrid (Tetric-Ceram Vivadent) composites placed on the etched (Liner Bond 2V Clearfil) cavities. Group B: Twin Pro, PQ1-Ultradent loaded Primer-Bonding plus fluid and micro-hybrid composites applied to the acid-etched (37% orthophosphoric acid) cavities. Group C: Twin Pro plus Silver amalgam alloy (Phasealloy- Sybram Kerr). Group D (control group): self-etching primer plus fluid and micro-hybrid composite. Specimens were investigated by SEM.


RESULTS
The results obtained in all groups show that the application of Twin Pro does not alter the adhesiveness of the restorative composite materials to the dental wall. In fact the desensitizer and the adhesive layers are indistinguishable at SEM observation, and well adherent to the dentinal surface of the cavities.


CONCLUSIONS
The results of SEM investigations show that Twin Pro does not decrease the adhesion of restorative materials to the tooth surface, as it establishes an efficient interconnection with the different materials used, and it forms a uniform layer covering and occluding dentine tubules, and this might constitute an efficacious sealing of dentinal tubules which possibly contribute to decrease dentinal sensitivity to environmental nociceptive stimuli.
Brenna
F
F
Dentistry and Stomatology Clinic, Faculty of Dentistry, Insubria University, School of Medicine, Varese, Italy.
Tagliabue
A
A
Levrini
L
L
Tettamanti
L
L
Quacci
D
D
Bergamaschi
M
M
D000180:Acrylic Resins / Q000627:therapeutic use; D001641:Bicuspid; D003188:Composite Resins / Q000627:therapeutic use; D003723:Dental Amalgam; D001840:Dental Bonding; D003736:Dental Cavity Lining; D003782:Dental Pulp / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000503:physiopathology / Q000648:ultrastructure; D003807:Dentin Sensitivity / Q000503:physiopathology / Q000628:therapy*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes / Q000627:therapeutic use; D012996:Solutions / Q000494:pharmacology*; D000267:Tissue Adhesions
D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D011140:Polyurethanes; D012996:Solutions; C513703:Vivadent; D003723:Dental Amalgam