title: Determination of specific interactions between glucose ligand carrying polymer and glucose transporter type-1 (GLUT-1) using different cell types.
abstract: In order to develop a biomimetic polymer for cell recognition, poly [3-O-(4'-vinylbenzyl)-D-glucose] (PVG) and different types of glucose transport (GLUT)-carrying cells, namely, HepG2 cells (GLUT-1), 3T3-L1 fibroblast cells (GLUT-1 and GLUT-4), and MIN6 cells (GLUT-2), were tested for specific interaction. To clarify the nature of interaction between PVG and the different cell types, rhodamine-B isothiocyanate (RITC)-labeled polymers were used to prove and visualize the interactions. In this study, we found that labeled polymer strongly binds to HepG2 cells. The fluorescence intensity of PVG with in the presence of HepG2 cells was found to be stronger than that of 3T3-L1 fibroblast cells (0.11 +/- 0.05) and MIN6 cells, which carry GLUT-2 (0.028 +/- 0.01); a confocal laser microscopic study confirmed this interaction. To confirm the specificity of the interaction mediated by GLUT, the cells were pretreated with phloretin, an inhibitor of GLUT-1, before adding RITC-labeled PVG polymer to the cell culture medium. This treatment suppressed the interaction of PVG with HepG2 cells and partially suppressed its interaction with 3T3-L1 fibroblast cells.
authors: Department of Biosystems and Agricultural Engineering, Chonnam National University, Kangjoo, Korea.
authors: Jong-Hoon
authors: JH
authors: Chung
authors: Keun-Hong
authors: KH
authors: Park
authors: Byong-Moo
authors: BM
authors: Seo
authors: Eun-Seok
authors: ES
authors: Kim
authors: Jong-Rak
authors: JR
authors: Hong
authors: Il-Hyuk
authors: IH
authors: Chung
authors: Nara
authors: N
authors: Kang
authors: Jeong-Hwa
authors: JH
authors: Baek
authors: Byung-Moo
authors: BM
authors: Min
authors: Yun-Hoon
authors: YH
authors: Choung
authors: Toshihiro
authors: T
authors: Akaike
authors: Pill-Houng
authors: PH
authors: Choung
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005453:Fluorescence; D005947:Glucose; D051272:Glucose Transporter Type 1; D006801:Humans; D008024:Ligands; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; D008024:Ligands; D009004:Monosaccharide Transport Proteins; D011137:Polystyrenes; C495677:SLC2A1 protein, human; C495678:Slc2a1 protein, mouse; C473338:poly(3-O-(4'-vinylbenzyl)-D-glucose); D005947:Glucose