Dissemination and spread of CTX-M extended-spectrum beta-lactamases among clinical isolates of Klebsiella pneumoniae in central China.
Of 50 extended-spectrum beta-lactamase-producing (ESBL) isolates of Klebsiella pneumoniae collected from six hospitals in Hubei Province, 20 were found to produce CTX-M ESBLs (40%). Sequence analysis of the six isolates carrying bla(CTX-M) genes revealed that they all harboured bla(CTX-M-3). In the majority of isolates, bla(CTX-M) genes were within large conjugative plasmids. A high degree of diversity of the RAPD types revealed that all the isolates carrying CTX-M were genetically unrelated and horizontal transfer of plasmids was probably the main mechanism of bla(CTX-M) spread. This is the first report of the occurrence of CTX-M-3 ESBLs in central China; previously this enzyme was identified only in Europe. A more comprehensive survey of ESBL types from China is urgently needed.
Li
Cong-Rong
CR
Department of Clinical Laboratory, RenMin Hospital of Wuhan University, Wuhan 430060, China.
Li
Yan
Y
Zhang
Pin-An
PA
D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D019105:Random Amplified Polymorphic DNA Technique; D018440:beta-Lactam Resistance / Q000502:physiology*; D001618:beta-Lactamases / Q000032:analysis*
C456079:beta-lactamase CTX-M-13; D001618:beta-Lactamases