Cell lineage assignment of cytogenetic findings in acute lymphoblastic leukemia using combined immunomagnetic cell separation and chromosome preparation.
In acute lymphoblastic leukemia (ALL) abnormal karyotypes frequently constitute a minor part of the dividing cells, and the origin of metaphases in normal diploid cases remains obscure. We used a combination of immunomagnetic cell separation and chromosome preparation (ICSCP) to focus on the metaphases of interest and to assign the chromosome findings to CD19+ or CD7+ leukemia cells.
Rieder
Harald
H
Bachmann
Ina
I
Busch
Sylke
S
Schwartz
Stefan
S
Reckzeh
Barbara
B
Hoelzer
Dieter
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D018941:Antigens, CD19 / Q000032:analysis*; D019011:Antigens, CD7 / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D001854:Bone Marrow Cells / Q000276:immunology / Q000473:pathology; D019070:Cell Lineage; D020223:Chromosome Painting; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping / Q000379:methods*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D018941:Antigens, CD19; D019011:Antigens, CD7; D000951:Antigens, Neoplasm