The labelling of Nanocoll with [111In] for dual-isotope scanning.
Visualization and biopsy of sentinel lymph nodes play an important role in planning and controlling the therapy of breast cancer. Hitherto two methods-scintigraphy or gamma probe detection after injection of [99mTc]-nanocolloids and visual detection after injection of patent blue dye-are used routinely. There are no conclusive publications elucidating such important parameters as injection site, injection method and colloidal parameters. The present work aims to label Nanocoll with [111In] to provide an alternative method, a simultanous one-compound dual-isotope application.


METHODS
[111In]-Indiumchloride was buffered with acetate and transferred to the nanocolloid. The colloid labelling reaction was complete after 30 min and filtrated through 100 nm Nuclepore filters.


RESULTS
Incorporation yield of [111In]-Indium into the nanocolloid was nearly quantitative, the step associated with the major loss of activity was the particle sizing with a mean yield of 55%.


CONCLUSIONS
The presented method allows for the routine supply of [111In]-nanocolloids. Size-filtered [111In]-Nanocoll shows the same particle size range as [99mTc]-Nanocoll.
Mitterhauser
Markus
M
Anstaltsapotheke des Allgemeinen Krankenhauses der Stadt Wien, Wien 1090, Austria. markus.mitterhauser@akh-wien.ac.at
Wadsak
Wolfgang
W
Mien
Leonhard-Key
LK
Eidherr
Harald
H
Roka
Sebastian
S
Zettinig
Georg
G
Angelberger
Peter
P
Viernstein
Helmut
H
Kletter
Kurt
K
Dudczak
Robert
R
D007205:Indium Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D010316:Particle Size; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013668:Technetium Tc 99m Aggregated Albumin / Q000737:chemistry*
D007205:Indium Radioisotopes; D019275:Radiopharmaceuticals; D013668:Technetium Tc 99m Aggregated Albumin; C083602:technetium Tc 99m nanocolloid