Comparison of UV and tandem mass spectrometric detection for the high-performance liquid chromatographic determination of diclofenac in microdialysis samples.
High-performance liquid chromatography (HPLC) was used to analyze microdialysis samples obtained in vivo from human subcutaneous adipose tissue after topical application of the nonsteroidal anti-inflammatory drug diclofenac. For the reliable determination of diclofenac two different detection principles were applied in two different laboratories. One HPLC method utilized UV-detection at 280 nm, the other one used selected reaction monitoring mass spectrometry (MS). The HPLC-UV and -MS methods offered low limits of quantification of 10 and 1 ng/ml and an accuracy between 94.0-126.7 and 89.3-110.9%, respectively. However, a comparison showed that the HPLC-UV method failed to determine diclofenac in biological matrices, as both false negative and positive values were found. HPLC-MS is clearly superior to HPLC-UV due to a much more selective detection, increased sensitivity and shorter run times.
Mayer
Bernhard X
BX
Department of Clinical Pharmacology, Division of Clinical Pharmacokinetics, Vienna University School of Medicine, Währinger Gürtel 18-20, 1090 Vienna, Austria. bernhard.mayer@univie.ac.at
Namiranian
Khodadad
K
Dehghanyar
Pejman
P
Stroh
Reinhard
R
Mascher
Hermann
H
Müller
Markus
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D016014:Linear Models; D017551:Microdialysis / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D004008:Diclofenac