An in vitro evaluation of the effects of homocysteine thiolactone on key steps of angiogenesis and tumor invasion.
Homocysteine thiolactone is a highly reactive homocysteine derivative that can react easily with proteins. Protein homocysteinylation has been suggested as a possible mechanism underlying the pathological consequences of impaired homocysteine metabolism. Homocysteine inhibits key steps of angiogenesis and tumor invasion. It can be hypothesized that homocysteine thiolactone could mimic the described anti-angiogenic and anti-invasive effects of homocysteine. Therefore, we studied the effects of homocysteine thiolactone on different key steps of angiogenesis and tumor invasion, using model endothelial and tumor cell lines. This study demonstrates that homocysteine thiolactone, in high contrast to homocysteine, is not an anti-angiogenic compound. Furthermore, our results suggest that homocysteine thiolactone could behave as a pro-angiogenic compound.
Martínez-Poveda
Beatriz
B
Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, E-29071 Málaga, Spain.
Chavarría
Teresa
T
Sánchez-Jiménez
Francisca
F
Quesada
Ana R
AR
Medina
Miguel Angel
MA
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000473:pathology; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000188:drug therapy*; D009389:Neovascularization, Pathologic; D011509:Proteoglycans / Q000494:pharmacology; D013997:Time Factors
D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors; D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D006710:Homocysteine; C059485:matrigel; D003094:Collagen; C007957:homocysteine thiolactone; D020780:Matrix Metalloproteinase 9