Correlation of biochemical and hematological changes with graft failure following pig heart and kidney transplantation in baboons.
We have explored biochemical and hematologic parameters that might indicate acute humoral xenograft rejection (AHXR) following pig organ transplantation in baboons. Baboons (n = 15) received an immunosuppressive regimen, and underwent a miniature swine or hDAF kidney (Group 1, n = 6) or heart (Group 2, n = 7) transplantation. Control baboons (Group 3, n = 2) received the immunosuppressive regimen without organ transplantation. Blood chemistry and hematologic parameters were measured daily. Baboon and porcine cytomegalovirus were monitored. In Groups 1 and 2, organ grafts survived for up to 29 days. A plasma fibrinogen of <80 mg/dL on 2 consecutive days, and a serum lactate dehydrogenase of >600 U/L and aspartate transaminase of >300 U/L, were associated with the development of AHXR in both heart and kidney grafts. In Group 1, a decrease in platelet count of >150,000/microL within 3 days, or a count of <50,000/microL, were associated with AHXR. In Group 2, a creatine phosphokinase of >500 U/L was associated with graft failure. In Group 3, no abnormalities were observed. The possibility that porcine CMV may play a role in graft injury could not be excluded. Noninvasive parameters were identified that have predictive potential for AHXR. Monitoring of these might enable therapeutic intervention to reverse rejection.
Knosalla
Christoph
C
Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.
Gollackner
Bernd
B
Bühler
Leo
L
Mueller
Nicolas J
NJ
Houser
Stuart
S
Mauiyyedi
Shamila
S
Sachs
David H
DH
Robson
Simon C
SC
Fishman
Jay
J
Schuurman
Henk-Jan
HJ
Awwad
Michel
M
Cooper
David K C
DK
D000818:Animals; D004211:Disseminated Intravascular Coagulation / Q000276:immunology; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010215:Papio / Q000276:immunology; D013552:Swine / Q000276:immunology; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000276:immunology*
D006454:Hemoglobins; D007770:L-Lactate Dehydrogenase