title: Dietary fat interacts with the -514C>T polymorphism in the hepatic lipase gene promoter on plasma lipid profiles in a multiethnic Asian population: the 1998 Singapore National Health Survey.
abstract: We have previously reported an interaction between -514C>T polymorphism at the hepatic lipase (HL) gene and dietary fat on high-density lipoprotein-cholesterol (HDL-C) metabolism in a representative sample of white subjects participating in the Framingham Heart Study. Replication of these findings in other populations will provide proof for the relevance and consistency of this marker as a tool for risk assessment and more personalized cardiovascular disease prevention. Therefore, we examined this gene-nutrient interaction in a representative sample of Singaporeans (1324 Chinese, 471 Malays and 375 Asian Indians) whose dietary fat intake was recorded by a validated questionnaire. When no stratification by fat intake was considered, the T allele was associated with higher plasma HDL-C concentrations (P = 0.001), higher triglyceride (TG) concentrations (P = 0.001) and higher HDL-C/TG ratios (P = 0.041). We found a highly significant interaction (P = 0.001) between polymorphism and fat intake in determining TG concentration and the HDL-C/TG ratio (P = 0.001) in the overall sample even after adjustment for potential confounders. Thus, TT subjects showed higher TG concentrations only when fat intake supplied >30% of total energy. This interaction was also found when fat intake was considered as continuous (P = 0.035). Moreover, in the upper tertile of fat intake, TT subjects had 45% more TG than CC individuals (P < 0.01). For HDL-C concentration, the gene-diet interaction was significant (P = 0.015) only in subjects of Indian origin. In conclusion, our results indicate that there are differences in the association of -514C>T polymorphism with plasma lipids according to dietary intake and ethnic background. Specifically, the TT genotype is associated with a more atherogenic lipid profile when subjects consume diets with a fat content > 30%.
authors: Department of Endocrinology, Singapore General Hospital, Singapore 169608.
authors: E Shyong
authors: ES
authors: Tai
authors: Dolores
authors: D
authors: Corella
authors: Mabel
authors: M
authors: Deurenberg-Yap
authors: Jeffery
authors: J
authors: Cutter
authors: Suok Kai
authors: SK
authors: Chew
authors: Chee Eng
authors: CE
authors: Tan
authors: Jose M
authors: JM
authors: Ordovas
mesh_terms: D044466:Asian People; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004041:Dietary Fats / Q000494:pharmacology*; D005006:Ethnicity; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India; D008049:Lipase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008296:Malaysia; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014280:Triglycerides / Q000097:blood
chemical_list: D017931:DNA Primers; D004041:Dietary Fats; D014280:Triglycerides; D008049:Lipase