Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica.
In this study an antifungal protein from Urginea indica bulbs was purified to homogeneity by acid precipitation, Diol 300 Gel-filtration, and C(18) reverse phase HPLC. Its molecular mass was estimated to be 29 kDa and periodic acid-Schiff (PAS) staining showed that identified antifungal molecule is a glycoprotein. The neutralization of antifungal activity after periodate oxidation of 29 kDa glycoprotein suggests that the glycan part of the molecule appears to be involved in antifungal activity. N-terminal amino acid sequence of the purified protein was determined as SQLKAXIXDF. This sequence had no sequence similarity with any antifungal proteins. A polyclonal antiserum was raised against purified protein and used in immunolocalization analysis. Results suggest that it is localized to the cell wall of the bulb. Antifungal tests have demonstrated that U. indica protein exerts a fungistatic effect. It completely inhibits the germination of spores and hyphal growth of Fusarium oxysporum.
Deepak
A V
AV
Department of Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India.
Thippeswamy
G
G
Shivakameshwari
M N
MN
Salimath
Bharathi P
BP
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000935:Antifungal Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005670:Fusarium / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry*; D019668:Liliaceae / Q000378:metabolism; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D018514:Plant Structures / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011817:Rabbits; D013997:Time Factors
D000906:Antibodies; D000935:Antifungal Agents; D002241:Carbohydrates; D006023:Glycoproteins; D010936:Plant Extracts; D010940:Plant Proteins