title: Nitric oxide synthase expressions in rat dorsal root ganglion after a hind limb tourniquet.
abstract: We investigated the mRNA levels of neuronal, inducible, endothelial nitric oxide synthases (nNOS, iNOS, eNOS) and tumor necrosis factor-alpha (TNF-alpha) in a rat dorsal root ganglion (DRG) after tourniquet application to a hind limb to identify molecules that trigger secondary events after peripheral nerve injury. Significantly high nNOS, iNOS mRNA and protein levels were observed in the ipsilateral DRGs 4 h after tourniquet application but not in the contralateral or control DRGs. The levels of TNF-alpha, an inducer of iNOS, were significantly increased in the ipsilateral DRGs 1 h after tourniquet application. Large amounts of NO might result in damage to the host cells and induce apotosis to eliminate damaged cells during the early stage of nerve injury.
authors: Department of Legal Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan. sumiko@fmu.ac.jp
authors: Sumiko
authors: S
authors: Abe
authors: Ikubumi
authors: I
authors: Mizusawa
authors: Katsuyuki
authors: K
authors: Kanno
authors: Atsuko
authors: A
authors: Yabashi
authors: Miwako
authors: M
authors: Suto
authors: Mikio
authors: M
authors: Kuraya
authors: Takashi
authors: T
authors: Honda
authors: Kouichi
authors: K
authors: Hiraiwa
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006614:Hindlimb / Q000201:enzymology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000201:enzymology; D014111:Tourniquets
chemical_list: D019001:Nitric Oxide Synthase