Isolation of pig pancreatic islets by a new method with hydraulic shaking: preliminary report.
The limited availability of human pancreas represents a serious problem in islet transplantation. In the past few years many efforts have been made to isolate pancreatic islets from large mammals in order to achieve valid and reproducible isolation methods. For several reasons swine may be considered an ideal source of islet tissue because of the similarity between human and porcine insulin and because of the easy availability of pig pancreata. Some papers have been published recently on this topic with good results. However, some problems, such as islet dissociation into single cells after collagenase digestion, are not completely solved. In this article, an automated method involving a hydraulic shaking system is described for islet isolation from the pig pancreas, developed in our laboratory and derived from Ricordi's model.
Viviani
G L
GL
Department of Endocrinology and Metabolism, University of Genova, Genova, Italy.
Fontana
I
I
Zecchi
A
A
Bottino
R
R
Sanna
R
R
Lione
L
L
Sacchi
G
G
Falzetti
G
G
Valente
U
U
Adezati
L
L
D000818:Animals; D002469:Cell Separation / Q000379:methods; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007511:Ischemia; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D014021:Tissue Preservation / Q000379:methods
D007328:Insulin