Changes in agouti-related peptide during the ring dove breeding cycle in relation to prolactin and parental hyperphagia.
Ring doves (Streptopelia risoria) exhibit marked increases in food consumption and decreases in body weight when they are provisioning their young. This study examined changes in hypothalamic immunostaining for agouti-related peptide (AGRP), an endogenous melanocortin receptor antagonist and appetite stimulant, during the ring dove breeding cycle. Because prolactin is orexigenic in doves, and is elevated in blood at the onset of parental hyperphagia, we also explored the possibility that prolactin-induced hyperphagia is associated with AGRP changes. The numbers of AGRP-immunoreactive (ir) cells within the tuberal hypothalamus were maximal during the prelaying period of the breeding cycle but decreased dramatically during early incubation. AGRP-ir cell numbers began to increase again during late incubation and reached a peak during the early and late posthatching stages. Because posthatching elevations in AGRP-ir were temporally associated with marked elevations in parental food intake, and because AGRP is orexigenic in doves, these findings suggest that increased AGRP activity in the dove tuberal hypothalamus may contribute to parental hyperphagia. Rising prolactin secretion during late incubation and early posthatching may initiate this increase in AGRP-ir, since intracerebroventricular administration of prolactin significantly elevated food intake and AGRP-ir cell numbers in the tuberal hypothalamus of nonbreeding doves. Prolactin-induced elevations in AGRP-ir cell numbers persisted when the confounding effects of weight gain that accompany prolactin-induced hyperphagia in nonbreeding doves were eliminated by a food restriction procedure, thereby suggesting that prolactin can directly influence AGRP activity under neutral energy state conditions.
Strader
A D
AD
University of Wisconsin-Milwaukee, Department of Biological Sciences, Milwaukee, WI, USA. april.strader@uc.edu
Buntin
J D
JD
D054369:Agouti-Related Protein; D000818:Animals; D010856:Columbidae / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D006963:Hyperphagia / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008425:Maternal Behavior / Q000502:physiology; D009425:Nesting Behavior / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D010332:Paternal Behavior; D011388:Prolactin / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D044101:Receptors, Melanocortin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012098:Reproduction / Q000502:physiology*
D054369:Agouti-Related Protein; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D044101:Receptors, Melanocortin; D011388:Prolactin