title: [Screening and identification of proteins interacting with p38 MAP kinase via T7 phage-display screening system].
abstract: OBJECTIVE
  To screen and identify proteins that interact with p38 mitogen-activated protein (MAP) kinases by means of T7 phage-display screening system.
  
  
  METHODS
  His-tagged fusion protein of p38 MAP kinase was used to coat a 96-well ELISA plate and Ni-NTA resin, which served as the media for screening human lung and liver T7 phage cDNA libraries.
  
  
  RESULTS
  After 4 rounds of biopanning, 86 independent plaques were selected and processed by EDTA. The inserted gene fragments from these plaques were amplified by PCR, the products purified by a gel recovery method. The sequences of the insertions were identified and analyzed with BLAST program in GenBank. Forty-six clones were found to encode proteins.
  
  
  CONCLUSIONS
  T7 phage-display screening system is convenient, rapid and effective for screening the P38 MAP kinase-binding proteins.
authors: Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China.
authors: Zhi-jie
authors: ZJ
authors: Li
authors: Jing-hua
authors: JH
authors: Liu
authors: Xiao-wei
authors: XW
authors: Gong
authors: Qing-he
authors: QH
authors: Qin
authors: Hao
authors: H
authors: Huang
authors: Peng
authors: P
authors: Deng
authors: Jing-zhen
authors: JZ
authors: Wang
authors: Shan-chao
authors: SC
authors: Zhao
authors: Xue-guang
authors: XG
authors: Sun
authors: Ya-wei
authors: YW
authors: Liu
authors: Ke-shen
authors: KS
authors: Zhao
authors: Yong
authors: Y
authors: Jiang
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011485:Protein Binding; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases