title: [Responses of neurons and astrocytes in rat hippocampus to kainic acid-induced seizures].
abstract: OBJECTIVE
  To investigate the time course of the responses of neurons and astrocytes in rat hippocampus (HI) to kainic acid (KA)-induced seizures in various regions.
  
  
  METHODS
  By means immunohistochemical staining for anti-Fos protein and anti-glial fibrillary acidic protein (GFAP), the regional distribution of reactive neurons and astrocytes in the HI was observed at different time points after a unilateral stereotaxic microinjection of KA into the lateral ventricle of rats to cause limbic and generalized convulsive seizures.
  
  
  RESULTS
  The injection of KA triggered limbic motor seizures including immobilization, staring, facial and jaw clonus ect. followed by recurrent generalized convulsive seizures. After KA-induced seizures, the GFAP-positive astrocytes and Fos-positive neurons were markedly increased in the HI. The increase of GFAP immunoreactivity was observed 30 min after the seizure onset, reaching the maximum at 1 h; the increase of Fos immunoreactivity was detected at 1 h after the onset, peaking at 2 h.
  
  
  CONCLUSIONS
  The neurons and astrocytes in rat HI are highly active during seizures and the reactive astrocytes might play an important role in epileptogenesis.
authors: Institute of Neurosciences of PLA and Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. zl-liu@163.net
authors: Zhi-liang
authors: ZL
authors: Liu
authors: Ru-xiang
authors: RX
authors: Xu
authors: Zhi-jun
authors: ZJ
authors: Yang
authors: Yi-wu
authors: YW
authors: Dai
authors: Cheng-yi
authors: CY
authors: Luo
authors: Mou-xuan
authors: MX
authors: Du
authors: Yu-xi
authors: YX
authors: Zou
authors: Xiao-dan
authors: XD
authors: Jiang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000473:pathology*; D007150:Immunohistochemistry; D007608:Kainic Acid; D008297:Male; D009474:Neurons / Q000473:pathology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000473:pathology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D016760:Proto-Oncogene Proteins c-fos; D007608:Kainic Acid