title: [Expression of pro-inflammation cytokines and activation of nuclear factor kappab in the intestinal mucosa of mice with ulcerative colitis].
abstract: OBJECTIVE
  To investigate the expression of pro-inflammation cytokines and activation of nuclear factor kappaB (NF-kappaB) in mouse models of ulcerative colitis.
  
  
  METHODS
  Mouse models of ulcerative colitis were established by oral administration of 5% dextran sulfate sodium for 7 d, and the expression of tumor necrosis factor (TNF)- alpha and interleukin (IL)-1beta in the intestinal mucosa were detected by semi-quantitative reverse transcriptional (RT) PCR. The activation of NF-kappaB in the intestinal mucosa was evaluated by electrophoretic mobility shift assay (EMSA).
  
  
  RESULTS
  The expressions of TNF-alpha and IL-1beta were increased in the intestinal mucosa (P=0.009), and the nuclear binding activity of NF-kappaB was also up-regulated after the onset of colitis.
  
  
  CONCLUSIONS
  Pro-inflammatory cytokines play important roles in the pathogenesis of UC, and may exacerbate the inflammation of the intestinal mocosa and cause apoptosis of the epithelial cells, possibly under the regulation of NF-kappaB activation.
authors: Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 50515, China.
authors: Qun-ying
authors: QY
authors: Wang
authors: Cun-long
authors: CL
authors: Chen
authors: Ji-de
authors: JD
authors: Wang
authors: Zhuo-sheng
authors: ZS
authors: Lai
authors: Qiang
authors: Q
authors: Ma
authors: Ya-li
authors: YL
authors: Zhang
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000209:etiology* / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics*; D005260:Female; D007375:Interleukin-1 / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha