title: Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens.
abstract: Three Herpes Simplex Virus LightCycler polymerase chain reaction assays were compared for the detection of herpes simplex virus in 48 swab specimens. The assays comprised of one in-house assay and two commercial kits: the Artus HSV LC RealArt PCR kit and the Roche LightCycler HSV 1/2 Detection kit. On the whole, the three assays had comparable sensitivities. However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis.
authors: Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Herston Road, 4029 Herston, Queensland, Australia.
authors: D M
authors: DM
authors: Whiley
authors: M W
authors: MW
authors: Syrmis
authors: I M
authors: IM
authors: Mackay
authors: T P
authors: TP
authors: Sloots
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000302:isolation & purification*; D012867:Skin / Q000821:virology; D013048:Specimen Handling
chemical_list: D004279:DNA, Viral