title: Expressed sequence tags from the Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, in the sexual reproduction process.
abstract: We obtained genetic information on sexual reproduction of the Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga. Normalized cDNA libraries were constructed from cells in the sexual reproduction process, and a total of 1190 5'-end expressed sequence tags were established. Since 604 of these ESTs were classified into 174 non-redundant sequences, these 1190 ESTs include 760 unique sequences. Similarity search against a public non-redundant protein database indicated that 390 unique sequences had significant similarity to registered sequences. Among these 390 sequences, 3 were identical to and 4 were homologous to previously identified sex-pheromone genes. According to our study, 370 of 760 unique sequences are likely to be novel transcripts. These cDNA clones and EST sequence information may would be helpful for future functional analyses using DNA array technologies.
authors: Institute of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan. sekimoto@bio.c.u-tokyo.ac.jp
authors: Hiroyuki
authors: H
authors: Sekimoto
authors: Yoichi
authors: Y
authors: Tanabe
authors: Mari
authors: M
authors: Takizawa
authors: Nao
authors: N
authors: Ito
authors: Ryo-hei
authors: RH
authors: Fukumoto
authors: Motomi
authors: M
authors: Ito
mesh_terms: D000595:Amino Acid Sequence; D003470:Culture Media; D018076:DNA, Complementary / Q000235:genetics; D030562:Databases, Protein; D056890:Eukaryota / Q000235:genetics*; D020224:Expressed Sequence Tags; D015723:Gene Library; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012724:Sex Attractants / Q000235:genetics
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D012724:Sex Attractants