Isolation and characterization of a zinc-containing metalloprotease expressed by Vibrio tubiashii.
A Vibrio tubiashii hemagglutinin, a protease, was purified by ammonium sulfate precipitation, gel filtration, and hydrophobic interaction chromatography. It agglutinates sheep, chicken, bovine, rabbit, guinea pig, and human erythrocytes. It has a molecular mass of 35 kDa, isoelectric points of 3.5 and 3.7, and is inhibited by ortho-phenanthro line, phosphoramidon, and Zincov. The N-terminal amino acid sequence (Ala-Gln-Ala-Thr-Gly-Thr-Gly- Pro-Gly-Gly-Asn-Gln-Lys-Thr-Gly-Gln- Tyr-Asn-Phe-Gly) has strong homology to other Vibrio proteases.
Delston
R B
RB
Division of Microbiological Studies, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740, USA.
Kothary
M H
MH
Shangraw
K A
KA
Tall
B D
BD
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002845:Chromatography / Q000379:methods; D006020:Glycopeptides / Q000494:pharmacology; D006384:Hemagglutination; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007526:Isoelectric Point; D045726:Metalloproteases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010618:Phenanthrolines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics; D015032:Zinc / Q000032:analysis
D006020:Glycopeptides; D006877:Hydroxamic Acids; D010618:Phenanthrolines; D011480:Protease Inhibitors; C048727:zincov; D045726:Metalloproteases; D015032:Zinc; D000645:Ammonium Sulfate; C008890:phosphoramidon