Control of effector CD8+ T cell function by the transcription factor Eomesodermin.
Activated CD8+ T cells play a critical role in host defense against viruses, intracellular microbes, and tumors. It is not clear if a key regulatory transcription factor unites the effector functions of CD8+ T cells. We now show that Eomesodermin (Eomes), a paralogue of T-bet, is induced in effector CD8+ T cells in vitro and in vivo. Ectopic expression of Eomes was sufficient to invoke attributes of effector CD8+ T cells, including interferon-gamma (IFN-gamma), perforin, and granzyme B. Loss-of-function analysis suggests Eomes may also be necessary for full effector differentiation of CD8+ T cells. We suggest that Eomesodermin is likely to complement the actions of T-bet and act as a key regulatory gene in the development of cell-mediated immunity.
Pearce
Erika L
EL
Abramson Family Cancer Research Institute, and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Mullen
Alan C
AC
Martins
Gislâine A
GA
Krawczyk
Connie M
CM
Hutchins
Anne S
AS
Zediak
Valerie P
VP
Banica
Monica
M
DiCioccio
Catherine B
CB
Gross
Darrick A
DA
Mao
Chai-An
CA
Shen
Hao
H
Cereb
Nezih
N
Yang
Soo Y
SY
Lindsten
Tullia
T
Rossant
Janet
J
Hunter
Christopher A
CA
Reiner
Steven L
SL
D000595:Amino Acid Sequence; D000818:Animals; D001117:Arenaviridae Infections / Q000276:immunology; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002454:Cell Differentiation; D003602:Cytotoxicity, Immunologic; D005786:Gene Expression Regulation; D053804:Granzymes; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics; D020825:T-Box Domain Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018418:Th2 Cells / Q000276:immunology / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D000098815:T-bet Transcription Factor
C491198:Eomes protein, mouse; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors; D054353:Perforin; D007371:Interferon-gamma; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases