Molecular evolution inferred from immunological cross-reactivity of immunoglobulin G among Chiroptera and closely related species.
We examined the relationships between Megachiroptera and Microchiroptera, and between Chiroptera and other closely related species by the cross-reactivity of immunoglobulin epitopes. Rabbit polyclonal antibody to bat IgG was used for determining the cross-reactivity by a competitive ELISA method. Megachiroptera and Microchiroptera showed high cross-reactivity, over 95.3%, with each other. However, primates and insectivores showed very low cross-reactivity, 8.6 to 20.2% and 5.3 to 12.7%, respectively. These results suggest that suborders of Chiroptera are monophyletic and Chiroptera have a relatively closer relationship to primates than to insectivores.
Omatsu
Tsutomu
T
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Ishii
Yoshiyuki
Y
Kyuwa
Shigeru
S
Milanda
Elizabeth G
EG
Terao
Keiji
K
Yoshikawa
Yasuhiro
Y
D000818:Animals; D002685:Chiroptera / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D003429:Cross Reactions / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D019143:Evolution, Molecular; D007074:Immunoglobulin G / Q000235:genetics* / Q000276:immunology*
D000939:Epitopes; D007074:Immunoglobulin G