Description of new mutations in the rpoB gene in rifampicin-resistant Neisseria meningitidis selected in vitro in a stepwise manner.
Fourteen meningococcal strains were selected towards rifampicin resistance in a stepwise manner in vitro; final MICs were between 8 and >256 microg ml(-1). Sequence analysis of a 295 bp subgenic fragment of the RNA polymerase beta-subunit (rpoB) gene from the original and the fully resistant strains revealed that, with one exception, the strain pairs differed by just one position in the deduced amino acid sequence. Transformation of a PCR-amplified subgenic rpoB fragment harbouring the mutated site into a susceptible strain demonstrated the resistance-conferring mechanism.
Nolte
Oliver
O
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
Müller
Matthias
M
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
Reitz
Stephan
S
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
Ledig
Sandra
S
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
Ehrhard
Ingrid
I
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
Sonntag
Hans-Günther
HG
Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany.
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D009154:Mutation; D009345:Neisseria meningitidis / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*; D014169:Transformation, Bacterial
D000904:Antibiotics, Antitubercular; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin