Helicobacter pylori infection influences expression of genes related to angiogenesis and invasion in human gastric carcinoma cells.
Infection with Helicobacter pylori (H. pylori) is considered a risk factor for gastric carcinoma. The purpose of this study was to clarify whether H. pylori infection plays a role in progression of gastric carcinoma. We examined the expression of genes encoding angiogenic factors and proteases by human gastric carcinoma cell lines (MKN-1 and TMK-1) co-cultured with or without H. pylori by cDNA microarray analysis. Co-culture with H. pylori increased expression of mRNAs encoding interleukin (IL)-8, vascular endothelial growth factor (VEGF), angiogenin, urokinase-type plasminogen activator (uPA), and metalloproteinase (MMP)-9 by gastric carcinoma cells. Up-regulation of these genes at the mRNA and protein levels was confirmed by Northern blot analysis, semi-quantitative RT-PCR analysis, and ELISA. In vitro angiogenic and collagenase activities of conditioned medium from the gastric carcinoma cells were also stimulated by co-culture with H. pylori. These results indicate that H. pylori infection may regulate angiogenesis and invasion of human gastric carcinoma.
Kitadai
Yasuhiko
Y
Department of Medicine and Molecular Science, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, 734-8551, Hiroshima, Japan. kitadai@hiroshima-u.ac.jp
Sasaki
Atsunori
A
Ito
Masanori
M
Tanaka
Shinji
S
Oue
Naohide
N
Yasui
Wataru
W
Aihara
Miki
M
Imagawa
Kenichi
K
Haruma
Ken
K
Chayama
Kazuaki
K
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D018196:Carcinoma, Adenosquamous / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D017364:Collagenases / Q000096:biosynthesis; D010450:Endopeptidases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
D043925:Angiogenesis Inducing Agents; D010450:Endopeptidases; D017364:Collagenases