A micromachined capillary electrophoresis chip with fully integrated electrodes for separation and electrochemical detection.
A novel analytical microsystem with fully integrated electrodes for electrophoresis and amperometrical detection is described. With respect to the lab-on-a-chip concept a capillary electrophoresis (CE) microsystem has been fabricated with a total of six gold electrodes for sample injection, separation and electrochemical detection using standard microfabrication technologies. The device is a ready-to-use system that does not need any extra mechanical apparatus for electrode insertion. The CE-chip has successfully been tested by measuring hydrogen peroxide, ascorbic acid and uric acid simultaneously. All three oxidizable species could be detected in less than 70 s. Glucose was detected by performing an enzymatic reaction along the separation channel. The microsystem showed a very good reproducibility.
Wilke
Ralph
R
Institute for Microtechnology, Technical University of Braunschweig, Alte Salzdahlumer Str. 203, 38124 Braunschweig, Germany. r.wilke@tu-bs.de
Büttgenbach
Stephanus
S
D001205:Ascorbic Acid / Q000032:analysis*; D045424:Complex Mixtures / Q000032:analysis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000032:analysis*
D045424:Complex Mixtures; D004800:Enzymes, Immobilized; D014527:Uric Acid; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D001205:Ascorbic Acid