A novel rabbit model for the evaluation of biomaterial associated urinary tract infection.
OBJECTIVE
It was the objective of this study to establish an animal model which simulates the conditions of a biomaterial associated bacterial urinary tract infection.


METHODS
The curled portion of polyurethane double pig-tail ureteric stents, pre-coated with P. aeruginosa,were inserted transurethrally into the bladder in eight rabbits. Eight control animals received sterile stent material. Microbiology studies of the stent, bladder tissue, and urine, as well as bladder histopathology were evaluated.


RESULTS
P. aeruginosa was recovered from all stent, bladder, and urine specimens in the P. aeruginosa pre-coated stent group, and no P. aeruginosa was present in any of the control specimens (p=0.0002). The controls only developed minimal bladder inflammation, whereas the bladders of the P. aeruginosa pre-coated stent group were significantly more inflamed (p<0.01).


CONCLUSIONS
This rabbit model was easy to manipulate, low in maintenance requirements, and had pathophysiologically distinct end points, suitable for the assessment of biomaterial associated urinary tract infections.
Fung
Leo C T
LC
Department of Urologic Surgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Mittelman
Marc W
MW
Thorner
Paul S
PS
Khoury
Antoine E
AE
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D018441:Biofilms; D004866:Equipment Contamination; D008297:Male; D023421:Models, Animal; D011140:Polyurethanes / Q000627:therapeutic use; D016459:Prosthesis-Related Infections; D011552:Pseudomonas Infections; D011817:Rabbits; D015607:Stents; D014552:Urinary Tract Infections / Q000382:microbiology*
D001672:Biocompatible Materials; D011140:Polyurethanes