[In vitro study of the effect of nm23-H1 on metastasis ability and chemo-sensitivity of Acc-M cell lines].
OBJECTIVE
To transfect nm23-H1 into the Acc-M cell lines in a safe, high-efficiency and low-toxicity way, and then to find out whether nm23-H1 affects the metastasis ability and chemo-sensitivity of Acc-M cell lines.


METHODS
Lipofect was used to transfect nm23-H1 into Acc-M cell lines. The difference in expression of nm23-H1 between the transfected and non-transfected cell lines was detected by immunohistochemistry. Then by use of transwell-room and wash way, the difference in invasion and metastasis ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of chemo-sensitivity.


RESULTS
Using pCMV-Neo-Bam system, we observed the stable expression of nm23-H1 and the significant difference in Nucleoside Diphosphate kinase-A, expression between the transfected and non-transfected Acc-M cell lines. The metastasis ability of transfected Acc-M cell lines decreased significantly. The chemo-sensitivity of transfected Acc-M cell line to cis-diamminedichloroplatin (C-DDP) increased significantly.


CONCLUSIONS
nm23-H1 can inhibit the metastases of Acc-M cell lines significantly and can increase the chemo-sensitivity to C-DDP significantly.
Chen
Shaowei
S
Department of Oral Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Liao
Xiangling
X
Pan
Jian
J
Yang
Kai
K
Hua
Chengge
C
Wen
Yuming
Y
D000818:Animals; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D051379:Mice; D009062:Mouth Neoplasms / Q000473:pathology*; D054778:NM23 Nucleoside Diphosphate Kinases; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism; D010714:Phosphatidylethanolamines; D011506:Proteins / Q000235:genetics*; D014162:Transfection
D054778:NM23 Nucleoside Diphosphate Kinases; D010714:Phosphatidylethanolamines; D011506:Proteins; C020888:1,2-dielaidoylphosphatidylethanolamine; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D002945:Cisplatin