A comparative study of the photophysical and phototoxic properties of octakis(decyloxy)phthalocyaninato zinc(II), incorporated in a hydrophilic polymer, in liposomes and in non-ionic micelles.
Factors such as charge, aggregation and lipophilicity influence photosensitiser localisation. The lipophilic octasubstituted sensitiser 2,3,9,10,16,17,23,24-octakis(decyloxy)phthalocyaninato zinc(II) was incorporated into liposomes of dimyristoyl-L-alpha-phosphatidylcholine (DMPC), non-ionic micelles of Tween 80 and the hydrosoluble polymer Solutol HS 15 in order to investigate how these different environments affect the photophysical properties and phototoxicity of the photosensitiser. Fluorescence quantum yields and singlet molecular oxygen generation are enhanced in the presence of Solutol HS 15. Phototoxicities were calculated by employing a concentration of 10(-7) M of the dye against the Hep-2 cell line, which showed a viability of 53 and 30% in DMPC and Solutol HS 15, respectively. After 24 h of photodynamic therapy with 15 min irradiation, apoptotic and necrotic cells were observed.
Rodriguez
Myriam E
ME
INQUIMAE, Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina.
Morán
Flavia
F
Bonansea
Analia
A
Monetti
Marisa
M
Fernández
Daniel A
DA
Strassert
Cristian A
CA
Rivarola
Viviana
V
Awruch
Josefina
J
Dicelio
Lelia E
LE
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine; D006801:Humans; D008081:Liposomes; D008823:Micelles; D009336:Necrosis; D009942:Organometallic Compounds / Q000737:chemistry* / Q000633:toxicity*; D018462:Photobiology; D010777:Photochemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000633:toxicity*; D011108:Polymers
D008081:Liposomes; D008823:Micelles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011108:Polymers; D004134:Dimyristoylphosphatidylcholine