title: Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers.
abstract: The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals. This led to the establishment of a "normal urine polypeptide pattern", consisting of 247 polypeptides, each of which was found in more than 50% of healthy individuals. Applying CE-MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern. Twenty-seven polypeptides were exclusively found in samples of patients. Another 13, present in controls, were missing. These data indicate that CE-MS can be applied as powerful tool in clinical diagnostics.
authors: Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.
authors: Stefan
authors: S
authors: Wittke
authors: Danilo
authors: D
authors: Fliser
authors: Marion
authors: M
authors: Haubitz
authors: Sebastian
authors: S
authors: Bartel
authors: Ronald
authors: R
authors: Krebs
authors: Frank
authors: F
authors: Hausadel
authors: Meike
authors: M
authors: Hillmann
authors: Igor
authors: I
authors: Golovko
authors: Peer
authors: P
authors: Koester
authors: Hermann
authors: H
authors: Haller
authors: Thorsten
authors: T
authors: Kaiser
authors: Harald
authors: H
authors: Mischak
authors: Eva M
authors: EM
authors: Weissinger
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000652:urine; D010455:Peptides / Q000652:urine*; D011507:Proteinuria / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010455:Peptides