Depolymerization of actin filament by cytochalasin E induces interleukin-8 production and up-regulates CD54 in the HeLa epithelial cell line.
We previously reported that the depolymerization of actin filament by cytochalasin E enhances low affinity Fcepsilon receptor II (CD23) expression on the human monocyte-like cell line, U937 (J. Clin. Immunol. 20: 235, 2000). In this study, we found that cytochalasin E strongly induces interleukin-8 through an epithelial cell line, HeLa, in dose- and time-dependent manners as assessed by enzyme-linked immunoassay and reverse transcription-polymerase chain reaction techniques. In addition, interleukin-8 production in the HeLa cells cultured with cytochalasin E was blocked in the presence of protein kinase C inhibitors, Go6976 and H-7. On the other hand, it was found that CD54 (intercellular adhesion molecule-1; ICAM-1) expression on the HeLa cells and the secretion of soluble CD54 were significantly up-regulated after culturing with cytochalasin E, and that these up-regulations of CD54 were also suppressed by Go6976. Taken together, these findings indicate that cytochalasin E activates protein kinase C under the depolymerization of actin filament, leading to the induction of interleukin-8 production and the up-regulation of CD54 in HeLa cells.
Ikewaki
Nobunao
N
Division of Immunology, Kyushu University of Health and Welfare, Faculty of Health and Science, Nobeoka, Miyazaki 882-8508, Japan.
Yamada
Akira
A
Inoko
Hidetoshi
H
D008841:Actin Cytoskeleton / Q000378:metabolism*; D016227:Benzoquinones; D002227:Carbazoles / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology*; D004791:Enzyme Inhibitors; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D016209:Interleukin-8 / Q000096:biosynthesis*; D047029:Lactams, Macrocyclic; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D015854:Up-Regulation
D016227:Benzoquinones; D002227:Carbazoles; D003572:Cytochalasins; D004791:Enzyme Inhibitors; D007211:Indoles; D016209:Interleukin-8; D047029:Lactams, Macrocyclic; D011809:Quinones; D018799:Intercellular Adhesion Molecule-1; C081021:Go 6976; D017828:Rifabutin; C007215:cytochalasin E; C020754:herbimycin; D011493:Protein Kinase C