Incubation of Schistosoma mansoni lung-stage schistosomula in corn oil exposes their surface membrane antigenic specificities.
Several potential cues for increased surface antigenic expression of lung-stage schistosomula, such as lack of glucose and amino acids and extremes of pH or HCO3- concentration, failed to alter the negligible larval reactivity with control, infection, or irradiated cercariae-vaccine serum in indirect membrane immunofluorescence. In contrast, incubation of larvae in 90% corn oil for 6 hr led to surface membrane changes, which allowed specific and strong binding of antibody from antischistosome sera. The data together indicated that the lung-stage worms' confinement of antigenic molecules in lipid-rich sites of the outer membrane could be reversed in vitro after exposure to corn oil, in a concentration- and time-dependent manner.
El Ridi
Rashika
R
Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt. rashika@mailer.eun.eg
Mohamed
Shadia H
SH
Tallima
Hatem
H
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D003314:Corn Oil; D006224:Cricetinae; D003470:Culture Media; D000939:Epitopes / Q000032:analysis*; D019084:Fluorescent Antibody Technique, Indirect; D007106:Immune Sera / Q000276:immunology; D008168:Lung / Q000469:parasitology*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology; D014612:Vaccines / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D003470:Culture Media; D000939:Epitopes; D007106:Immune Sera; D014612:Vaccines; D003314:Corn Oil