title: Accurate analysis of fluorescence decays from single molecules in photon counting experiments.
abstract: In time-resolved, single-photon counting experiments, the standard method of nonlinear least-squares curve fitting incorrectly estimates the fluorescence lifetimes. Even for single-exponential data, errors may be up to +/- 15%, and for more complex fits, may be even higher, although the fitted line appears to describe the data. The origin of this error is not a result of the Poisson distribution, as is often stated, but is entirely due to the weighting of the fit. An alternative weighting method involving a minor change in the fitting method eliminates this problem, enabling accurate fitting even in difficult cases, including the small data sets observed in single molecule experiments and with a precision similar to that of maximum likelihood methods.
authors: Department of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors: David A
authors: DA
authors: Turton
authors: Gavin D
authors: GD
authors: Reid
authors: Godfrey S
authors: GS
authors: Beddard