[Effect of leptin in the production of progesterone and estradiol by ovarian rat tissue].
An alteration in the balance of autocrine/paracrine ovarian factors may lead to different diseases. The aim of the present report was to evaluate the relationship between insulin growth factor 1 (IGF-1) and leptin (Lp) in the progesterone (P) and estradiol (E) production after ovarian rat FSH stimulation. Thus, ovarian explants were incubated with FSH, IGF-1 and Lp, and P and E were evaluated by means of specific radioimmunoassays. FSH increased ovarian P and E production. IGF-1 synergized with FSH in P as well as in ovarian E production. Lp had not shown any effect on the FSH capacity to increase P and E in the ovarian tissue; nevertheless it reverted the synergistic effect of IGF-1 on FSH for P as well as for E. These data raise the possibility that abnormal Lp levels may contribute to infertility in women with polycystic ovarian syndrome by counteracting the sensitizing effects of IGF-1 in ovarian tissue.
Rearte
María B
MB
Centro de Estudios Farmacológicos y Botánicos (CEFYBO- CONICET), Facultad de Medicina, Universidad de Buenos Aires.
Luchetti
Carolina G
CG
Sander
Valeria
V
González
Claudio
C
Di Girolamo
Guillermo
G
Motta
Alicia B
AB
D000704:Analysis of Variance; D000818:Animals; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D020738:Leptin / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D020738:Leptin; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone