[Expression of pro-inflammation cytokines and activation of nuclear factor kappab in the intestinal mucosa of mice with ulcerative colitis].
OBJECTIVE
To investigate the expression of pro-inflammation cytokines and activation of nuclear factor kappaB (NF-kappaB) in mouse models of ulcerative colitis.


METHODS
Mouse models of ulcerative colitis were established by oral administration of 5% dextran sulfate sodium for 7 d, and the expression of tumor necrosis factor (TNF)- alpha and interleukin (IL)-1beta in the intestinal mucosa were detected by semi-quantitative reverse transcriptional (RT) PCR. The activation of NF-kappaB in the intestinal mucosa was evaluated by electrophoretic mobility shift assay (EMSA).


RESULTS
The expressions of TNF-alpha and IL-1beta were increased in the intestinal mucosa (P=0.009), and the nuclear binding activity of NF-kappaB was also up-regulated after the onset of colitis.


CONCLUSIONS
Pro-inflammatory cytokines play important roles in the pathogenesis of UC, and may exacerbate the inflammation of the intestinal mocosa and cause apoptosis of the epithelial cells, possibly under the regulation of NF-kappaB activation.
Wang
Qun-ying
QY
Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 50515, China.
Chen
Cun-long
CL
Wang
Ji-de
JD
Lai
Zhuo-sheng
ZS
Ma
Qiang
Q
Zhang
Ya-li
YL
D000818:Animals; D003093:Colitis, Ulcerative / Q000209:etiology* / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics*; D005260:Female; D007375:Interleukin-1 / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D007375:Interleukin-1; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha