Autometallographic localization of heavy metals in resin sections, cryosections and isolated cells of the mussel Mytilus galloprovincialis (L.).
In the present study, autometallography (AMG) has been performed on resin-embedded tissue sections, cryosections, as well as on isolated cells from the digestive gland, the gills and the haemolymph of mussels (Mytilus galloprovincialis) collected from the field. The AMG reaction was more intense in cryosections and isolated cells compared to resin sections. This intense AMG reaction found in cryosections and in isolated cells and the time effectiveness of the procedures enhances their use in a variety of studies, such as biomonitoring, even if small amount of metals are to be detected. In addition, the preparation of isolated cells does not require specific instruments or qualified personnel and, thus, the use of isolated cells is encouraging for AMG applications, although further laboratory investigation is required. On the other hand, the use of resin-embedded tissue sections for AMG applications, even though it is complicated and time consuming, leads to a high preservation of structural morphology and allows the exact localization of metals in discrete cellular compartments.
Raftopoulou
E K
EK
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
Domouhtsidou
G P
GP
Dimitriadis
V K
VK
D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000378:metabolism*; D002451:Cell Compartmentation / Q000502:physiology; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D004853:Epoxy Resins / Q000737:chemistry; D005088:Exocrine Glands / Q000737:chemistry / Q000378:metabolism; D005629:Frozen Sections / Q000379:methods*; D005880:Gills / Q000737:chemistry / Q000378:metabolism; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism*; D016613:Plastic Embedding / Q000379:methods*
D004853:Epoxy Resins; D019216:Metals, Heavy; C048709:spurr resin