Nitric oxide synthase expressions in rat dorsal root ganglion after a hind limb tourniquet.
We investigated the mRNA levels of neuronal, inducible, endothelial nitric oxide synthases (nNOS, iNOS, eNOS) and tumor necrosis factor-alpha (TNF-alpha) in a rat dorsal root ganglion (DRG) after tourniquet application to a hind limb to identify molecules that trigger secondary events after peripheral nerve injury. Significantly high nNOS, iNOS mRNA and protein levels were observed in the ipsilateral DRGs 4 h after tourniquet application but not in the contralateral or control DRGs. The levels of TNF-alpha, an inducer of iNOS, were significantly increased in the ipsilateral DRGs 1 h after tourniquet application. Large amounts of NO might result in damage to the host cells and induce apotosis to eliminate damaged cells during the early stage of nerve injury.
Abe
Sumiko
S
Department of Legal Medicine, Fukushima Medical University School of Medicine, Fukushima, Japan. sumiko@fmu.ac.jp
Mizusawa
Ikubumi
I
Kanno
Katsuyuki
K
Yabashi
Atsuko
A
Suto
Miwako
M
Kuraya
Mikio
M
Honda
Takashi
T
Hiraiwa
Kouichi
K
D000818:Animals; D005727:Ganglia, Spinal / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006614:Hindlimb / Q000201:enzymology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000201:enzymology; D014111:Tourniquets
D019001:Nitric Oxide Synthase