[Cloning and sequence analysis of Eg95 cDNA from different stages of Echinococcus granulosus in Xinjiang].
OBJECTIVE
To study expression and sequence differences of Echinococcus granulosus 95(Eg95) antigen cDNA from different stages of protoscolex, oncosphere and adult worm of E. granulosus from Xinjiang Uighur Aut. Reg.


METHODS
In accordance with the sequence of Eg95 antigen cDNA, the primers of Eg95 were designed. Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.


RESULTS
PCR results showed that Eg95 antigen cDNA was amplified from three stages of E. granulosus cDNA libraries. Sequencing analysis indicated that the Eg95 cDNA length was 402 bp, same as the reported data in GenBank.


CONCLUSIONS
The Eg95 antigen cDNA was expressed in the different life-cycle stages of E. granulosus in Xinjiang and there was no nucleic acid sequence difference of Eg95 antigen among the protoscolex, oncosphere and adult worm of E. granulosus.
Lin
Ren-yong
RY
Xinjiang Hydatid Fundamental Medical Key Lab, 1st Teaching Hospital of Xinjiang Medical University, Urumqi 830054.
Ding
Jian-bing
JB
Wen
Hao
H
Zhang
Wen-bao
WB
Li
Jun
J
Lu
Xiao-mei
XM
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004446:Echinococcus / Q000235:genetics* / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D000947:Antigens, Helminth; D018076:DNA, Complementary; C446034:EG95 protein, Echinococcus granulosus; D015801:Helminth Proteins