Characterization of gdp1+ as encoding a GDPase in the fission yeast Schizosaccharomyces pombe.
We have isolated the gdp1+ gene from Schizosaccharomyces pombe coding for a membrane protein with guanosine diphosphatase (GDPase) activity, which is highly homologous to Golgi GDPases isolated from other yeast species. The gdp1+ product, Gdp1p, displays both GDPase and uridine diphosphatase (UDPase) activities in vitro, with a strong dependence for calcium and manganese cations. The observation of a defect in N-glycosylation of invertase in S. pombe Deltagdp1 cells together with the ability of gdp1+ to functionally complement the defective O-mannosylation of chitinase in Saccharomyces cerevisiae cells disrupted in the GDA1 gene (gdp1+ homolog), suggests a main role for Gdp1p in protein glycosylation in fission yeast.
Sánchez
Raquel
R
Department of Genetics and Microbiology, Faculty of Biology, Universidad de Murcia, 30071 Murcia, Spain.
Franco
Alejandro
A
Gacto
Mariano
M
Notario
Vicente
V
Cansado
José
J
D006031:Glycosylation; D016296:Mutagenesis; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
D029702:Schizosaccharomyces pombe Proteins; D011755:Pyrophosphatases; C023273:uridine diphosphatase; C010357:guanosine-diphosphatase