Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, gram stain-negative blood cultures.
Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.
Adler
Hildegard
H
Bacteriology Laboratory, University of Basel Hospitals and Clinics, 4031 Basel, Switzerland. hadler@uhbs.ch
Baumlin
Nathalie
N
Frei
Reno
R
D000165:Acridine Orange; D001769:Blood / Q000382:microbiology; D005189:False Positive Reactions; D005456:Fluorescent Dyes; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D015203:Reproducibility of Results
D005456:Fluorescent Dyes; D000165:Acridine Orange