Identification of novel HLA class I alleles using single allele sequencing.
Three new HLA-A and five HLA-B alleles reported in this paper have been characterized by direct sequencing of PCR product obtained by group-specific amplification of potential new alleles. Four new alleles, B*5133, B*5134, B*1574 and B*5807, carry motifs observed in previously identified HLA-B alleles and may have evolved via gene conversion. Four alleles, A*2438, A*3405, A*2437 and B*520104, display polymorphisms at positions previously considered constant. All new alleles were identified either by an unexpected sequence specific oligonucleotide probe hybridization pattern or by sequence-based typing, and later confirmed by single allele sequencing.
Lebedeva
T V
TV
HLA Laboratory, American Red Cross Blood Services, New England Region, Dedham, MA 02026, USA.
Huang
A
A
Janzen
M
M
Lau
M
M
Yu
N
N
D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D017422:Sequence Analysis, DNA
D015234:HLA-A Antigens; D015235:HLA-B Antigens