Development of a dipstick dye immunoassay for diagnosing hydatidosis.
We have developed a kit to diagnose hydatidosis, based on the detection of specific antibodies. This disease, caused by larvae of the cestode Echinococcus granulosus, is a zoonosis commonly found throughout the world. The diagnostic kit discriminates between positive and negative cases in a way that is easily interpreted, i.e., positive cases form a colored band on an inert substrate. The results show test sensitivity to be some 94.87% and specificity 85.71%, which is comparable to those of the ELISA technique. The high sensitivity and specificity of this test affords the great advantage of speed in diagnosing this parasitosis. No other equipment or procedure is required.
Sbihi
Younes
Y
Instituto de Biotecnología, Campus Fuentenueva, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
Gil
José R
JR
Alvarez
Pedro A
PA
Orduña
Antonio
A
Rodríguez-Torres
Antonio
A
Osuna
Antonio
A
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology; D004396:Coloring Agents; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology; D004446:Echinococcus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004396:Coloring Agents; D011933:Reagent Kits, Diagnostic