Comparison of Etest with modified broth microdilution method for testing susceptibility of Aspergillus spp. to voriconazole.
We compared the Etest with a broth microdilution method, performed according to a modified National Committee for Clinical Laboratory Standards guideline (M38-A), for determining the in vitro susceptibility of 77 isolates of Aspergillus spp. (26 A. fumigatus, 21 A. flavus, 10 A. terreus, 9 A. niger, 5 A. nidulellus, 4 A. glaucus, and 2 A. flavipes isolates). Overall, there was 92.2% agreement between both methods when Etest MICs were read at 24 h and 83.1% agreement when both methods were read at 48 h. When Etest MICs were read at 24 h, the agreement was >90% for all species tested except for A. fumigatus (84.6%). When Etest MICs were read at 48 h, the agreement ranged from 50 to 100%. The poorest agreement was seen with A. glaucus (50%) and A. fumigatus (65%). Where a discrepancy was observed between Etest and the reference method, the Etest MIC was generally higher. The Etest appears to be a suitable alternative procedure for testing the susceptibility of Aspergillus spp. to voriconazole.
Serrano
M C
MC
Servicio de Microbiología Clínica, Hospital Universitario de Valme, Seville 41014, Spain.
Morilla
D
D
Valverde
A
A
Chávez
M
M
Espinel-Ingroff
A
A
Claro
R
R
Ramírez
M
M
Mazuelos
E Martín
EM
D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000145:classification / Q000382:microbiology; D001230:Aspergillus / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D001231:Aspergillus flavus / Q000187:drug effects; D001232:Aspergillus fumigatus / Q000187:drug effects; D001234:Aspergillus niger / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011743:Pyrimidines / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D065819:Voriconazole