title: Toxicity and kinetics of methoxyfenozide in greenhouse-selected Spodoptera exigua (Lepidoptera: Noctuidae).
abstract: Methoxyfenozide (RH-2485) was found to be 7.5-fold less toxic in terms of LD50 values against last-instar larvae of a greenhouse-selected strain of the beet armyworm Spodoptera exigua (Hübner) that was collected in July 2001 in an experimental greenhouse for resistance at Murcia in southern Spain, in comparison with a laboratory susceptible strain. To date, the compound is the newest member of this new group of moulting hormone accelerating IGRs to reach the marketplace against Lepidoptera. To understand this different potency in the greenhouse-selected S exigua, oxidative metabolism and acetylcholinesterase activities were measured in last-instar larvae and adults. In addition, we determined, by the use of 14C-labelled methoxyfenozide, the pattern of absorption in body tissues and excretion via faeces in last-instar larvae of the greenhouse-selected strain and compared the results with those from the laboratory susceptible strain. It was striking that the rate of excretion was about twice as high in the greenhouse-selected strain, resulting in a more rapid clearance of insecticide amounts from the insect body. Data are discussed in relation to mechanisms of lower toxicities for this new group of IGRs.
authors: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Belgium. guy.smagghe@rug.ac.be
authors: Guy
authors: G
authors: Smagghe
authors: Samuel
authors: S
authors: Pineda
authors: Bert
authors: B
authors: Carton
authors: Pedro
authors: P
authors: Del Estal
authors: Flor
authors: F
authors: Budia
authors: Elisa
authors: E
authors: Viñuela
mesh_terms: D000042:Absorption; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D002250:Carbon Radioisotopes; D005243:Feces / Q000737:chemistry; D006834:Hydrazines / Q000493:pharmacokinetics* / Q000633:toxicity*; D007300:Insect Control; D007305:Insecticide Resistance; D007306:Insecticides; D007605:Juvenile Hormones / Q000493:pharmacokinetics* / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D018411:Spodoptera / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D006834:Hydrazines; D007306:Insecticides; D007605:Juvenile Hormones; C406712:methoxyfenozide; D000110:Acetylcholinesterase