Development of an IL-6 antagonist peptide that induces apoptosis in 7TD1 cells.
Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of proliferation and differentiation of hematopoietic cells and in the pathogenesis of many diseases, including multiple myeloma. This study pursues a way to interfere with IL-6 pathway in an attempt to modulate its biological activity. Here we describe the rational design and biological evaluation of peptides able to antagonize the murine IL-6 activity by interfering with IL-6 Receptor alpha in 7TD1 cells, a IL-6-dependent B-cell line. Of the peptide tested, only Guess 4a is capable of interfering with IL-6 transducing pathway, therefore inducing growth arrest and apoptosis of 7TD1 cells.
Manfredini
Rossella
R
Sezione di Chimica Biologica, Dipartimento di Scienze Biomediche, Università degli Studi di Modena e Reggio Emilia, Via Campi 297, 41100, Modena, Italy.
Tenedini
Elena
E
Siena
Michela
M
Tagliafico
Enrico
E
Montanari
Monica
M
Grande
Alexis
A
Zanocco-Marani
Tommaso
T
Poligani
Cristina
C
Zini
Roberta
R
Gemelli
Claudia
C
Bergamaschi
Anna
A
Vignudelli
Tatiana
T
De Rienzo
Francesca
F
De Benedetti
Pier Giuseppe
PG
Menziani
Maria Cristina
MC
Ferrari
Sergio
S
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D019295:Computational Biology; D003198:Computer Simulation; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D000093242:Interleukin-6 Inhibitors
D015850:Interleukin-6; D010455:Peptides; D000093242:Interleukin-6 Inhibitors