Alterations in mitochondria function and morphology in HT29 cells upon conditions inducing differentiation and apoptosis.
Morphological and biochemical studies of HT29 cells treated with sodium butyrate and/or glucose-deprived revealed both apoptotic and differentiation response. The main apoptotic response was accompanied with an increase of floating cells. However, the ultrastructural analysis of adherent cells showed the typical apoptotic character of the nucleus in some of them. In addition, remarkable changes of mitochondria, assumed as early stages starting the apoptotic cascade, were observed. These changes were represented not only by alterations of mitochondrial morphology, but also by the number of mitochondria and their localization.
Sovová
Vlasta
V
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 37 Praha 6, Czech Republic.
Stokrová
Jitka
J
Sloncová
Eva
E
Kucerová
Dana
D
Tuhácková
Zdena
Z
Korb
Jan
J
D018686:Anesthetics, Intravenous / Q000494:pharmacology; D017209:Apoptosis; D015153:Blotting, Western; D002454:Cell Differentiation; D003593:Cytoplasm / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D012978:Sodium Oxybate / Q000494:pharmacology; D013347:Subcellular Fractions
D018686:Anesthetics, Intravenous; D012978:Sodium Oxybate; D005947:Glucose