Encapsulated yeast cells inside Paramecium primaurelia: a model system for protection capability of polyelectrolyte shells.
One of the most promising applications of encapsulated living cells is their use as protected transplanted tissue into the human body. A suitable system for the protection of living cells is the use of nano- or microcapsules of polyelectrolytes. These shells can be deposited easily on top of the cells by means of a layer-by-layer technique. An interesting feature of the capsules is the possibility to control their properties on a nanometre level, tuning their wall texture via the preparation conditions. Here we introduce a model system to test the protection ability of polyelectrolyte capsules. Common bakery yeast cells were encapsulated. They were coated with a fluorescently labelled shell at conditions known to guarantee cell survival, and the cell interior was stained with DAPI. The protozoan Paramecium primaurelia was incubated with this double-stained living yeast and visualized by means of two-photon excitation fluorescence microscopy. Cross-sections of the dye-stained material as well as autofluorescence of the fixed protozoan allowed us to follow the digestion of the coated yeast with time. Our investigation reveals that capsules prepared under these deposition conditions are permeable to lysosomal enzymes, leading to degradation of the yeast inside the intact capsules. Our preliminary results indicate the suitability of the introduced model as a test system of this permeability.
Krol
S
S
INFM, Department of Physics, and DIPTERIS, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy. krol@fisica.unige.it
Cavalleri
O
O
Ramoino
P
P
Gliozzi
A
A
Diaspro
A
A
D000818:Animals; D002214:Capsules; D010450:Endopeptidases / Q000378:metabolism; D007211:Indoles; D008049:Lipase / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010247:Paramecium / Q000382:microbiology*; D010539:Permeability; D011073:Polyamines; D011137:Polystyrenes; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000502:physiology
D002214:Capsules; D007211:Indoles; D011073:Polyamines; D011137:Polystyrenes; C063994:polyallylamine; C007293:DAPI; C003321:polystyrene sulfonic acid; D008049:Lipase; D010450:Endopeptidases