The relationship between activities of hepatic and gastric alcohol dehydrogenase and occurrence of chronic alcoholic liver disease.
OBJECTIVE
To investigate the role of hepatic and gastric alcohol dehydrogenase (ADH) in different pathologic stages of alcoholic liver disease (ALD).


METHODS
Thirty-nine Wistar rats were divided randomly into two groups: model group (24) and control group (15). The ALD model was established by infusing alcohol into the stomach. After hepatic and gastric tissues had been stained by enzyme histo-cytochemistry assay, the activity varieties of hepatic and gastric ADH were observed by an optical microscope, and the activity alterations were also determined by LUZEX-F image analysis as a semi-quantitative method.


RESULTS
The activity of hepatic ADH gradually increased, but that of gastric ADH gradually decreased in the different pathologic stages of alcoholic liver disease. There was a significant difference between the model group and control group (P<0.05).


CONCLUSIONS
Along with occurrence of ALD, the activity of hepatic ADH gradually increased, but that of gastric ADH gradually decreased, showing that the activity alterations of hepatic and gastric ADH may play an important role in the onset and development of ALD.
Wang
Guo-Xiang
GX
Digestive Division, Department of Internal Medicine, General Hospital of Benxi Steel-Iron Company, Benxi 117000, China.
Wang
Bing-Yuan
BY
Liu
Chun-Rong
CR
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000818:Animals; D002908:Chronic Disease; D006651:Histocytochemistry / Q000379:methods; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013270:Stomach / Q000201:enzymology* / Q000473:pathology
D000426:Alcohol Dehydrogenase