title: Accumulation of LEA proteins in salt (NaCl) stressed young seedlings of rice (Oryza sativa L.) cultivar Bura Rata and their degradation during recovery from salinity stress.
abstract: Germination and subsequent hydroponic growth under salt stress (100 mmol/L NaCl) triggered an accumulation of six major stress proteins and resulted in a growth arrest of young seedlings of rice (Oryza sativa L.) cv. Bura Rata. Based on two-dimensional electrophoretic resolution, partial amino acid sequencing and immunodetection techniques, four of the salt stress-induced polypeptides were identified as LEA proteins. Under all experimental conditions wherein seedlings exhibited superior halotolerance, salt stress-induced LEA proteins were expressed at low levels. In contrast, accumulation of LEA proteins was found associated with growth arrest. When returned to non-saline media, seedlings stressed with salt for four days recovered immediately. Longer exposure to 100 mmol/L NaCl, however, progressively delayed recovery and reduced the number of seedlings which could recover from salt stress. Recovery from salt stress was consistently accompanied by degradation of the salt stress-induced LEA proteins. The results of this study show that LEA proteins accumulate during the salinity-triggered growth arrest of young Bura Rata seedlings and are mobilised during the recovery of seedlings from salinity stress.
authors: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India.
authors: Karuna
authors: K
authors: Chourey
authors: Saradha
authors: S
authors: Ramani
authors: Shree Kumar
authors: SK
authors: Apte
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D012965:Sodium Chloride