Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers.
The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals. This led to the establishment of a "normal urine polypeptide pattern", consisting of 247 polypeptides, each of which was found in more than 50% of healthy individuals. Applying CE-MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern. Twenty-seven polypeptides were exclusively found in samples of patients. Another 13, present in controls, were missing. These data indicate that CE-MS can be applied as powerful tool in clinical diagnostics.
Wittke
Stefan
S
Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.
Fliser
Danilo
D
Haubitz
Marion
M
Bartel
Sebastian
S
Krebs
Ronald
R
Hausadel
Frank
F
Hillmann
Meike
M
Golovko
Igor
I
Koester
Peer
P
Haller
Hermann
H
Kaiser
Thorsten
T
Mischak
Harald
H
Weissinger
Eva M
EM
D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000652:urine; D010455:Peptides / Q000652:urine*; D011507:Proteinuria / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010455:Peptides