title: Increased serum malondialdehyde and decreased nitric oxide within 24 hours of thrombotic stroke onset.
abstract: OBJECTIVE
  Ischemia/reperfusion generates free oxygen radicals, which react with the unsaturated lipids of biomembranes resulting in the generation products such as malondialdehyde. Malondialdehyde could be a sensor for tissue damage and reperfusion. Nitric oxide, released due to the early arrival of leukocytes in the brain parenchyma, could be a sensor for nonflow phenomenon. Thereby, the purpose of this research was to evaluate the behavior of malondialdehyde and nitric oxide within the 24 hours after the stroke onset.
  
  
  METHODS
  Fifteen patients up to an age of 49 years, admitted to the emergency of University Hospital and Chiquinquirá Hospital in Maracaibo, Venezuela, were examined by a neurologist and underwent 12-lead electrocardiograms and computed tomography for the diagnosis of thrombotic stroke. Serum malondialdehyde and nitric oxide were measured as thiobarbituric acid adducts and total nitrites. Data were collected within the 24 hours after the stroke onset.
  
  
  RESULTS
  Malondialdehyde for patients with stroke had a significant increase (P<0.001) when compared with healthy controls (47.9 +/- 7.1 vs. 1.7 +/- 0.2 micromol/L). Conversely, serum nitric oxide for patients with stroke had a significant decrease (P<0.001) when compared with the control group (14.5 +/- 1.4 vs. 41.3 +/- 3.7 micromol/L). The lowest values of malondialdehyde and the highest values of nitric oxide were observed in two patients, who died.
  
  
  CONCLUSIONS
  Serum levels of malondialdehyde increase, and serum levels of nitric oxide diminish within 24 hours after the onset of thrombotic stroke onset. This suggests that serum malondialdehyde level could be used as potentially reliable and sensitive marker for reperfusion, whereas nitric oxide levels could acts as potential biochemical sensor for nonreflow phenomenon.
authors: Centro de Investigaciones Endocrino-Metabólicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela. climacoc@hotmail.com
authors: Climaco P
authors: CP
authors: Cano
authors: Valmore P
authors: VP
authors: Bermúdez
authors: Hernán E
authors: HE
authors: Atencio
authors: Mayerlim T
authors: MT
authors: Medina
authors: Amell
authors: A
authors: Anilsa
authors: Aida
authors: A
authors: Souki
authors: Omaira M
authors: OM
authors: Molina
authors: Helen
authors: H
authors: Restrepo
authors: Maria E
authors: ME
authors: Vargas
authors: Maryluz
authors: M
authors: Núñez
authors: Merlyn
authors: M
authors: Ambard
authors: Abdón
authors: A
authors: Toledo
authors: Freddy
authors: F
authors: Contreras
authors: Manuel
authors: M
authors: Velasco
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020767:Intracranial Thrombosis / Q000097:blood* / Q000150:complications; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D012016:Reference Values; D020521:Stroke / Q000097:blood* / Q000209:etiology; D013997:Time Factors; D014687:Venezuela
chemical_list: D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde