Purification of alpha2-macroglobulin and the construction of immunogenic alpha2-macroglobulin-peptide complexes for use as cancer vaccines.
A simple method for purification of immunogenic alpha2-macroglobulin from murine serum is described. Also described is a method for creating alpha2-macroglobulin-antigen complexes using purified alpha2-macroglobulin and peptides derived from tumor cell lysates. The application of such alpha2-macroglobulin-antigen complexes for eliciting cancer immunity is discussed.
Binder
Robert J
RJ
Center for Immunotherapy of Cancer and Infectious Diseases, University of Connecticut School of Medicine, Farmington, CT 06030-1601, USA. Rbinder@up.uchc.edu
D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology*; D019496:Cancer Vaccines / Q000138:chemical synthesis* / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D000511:alpha-Macroglobulins / Q000276:immunology* / Q000302:isolation & purification
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D010455:Peptides; D000511:alpha-Macroglobulins