Effects of a novel anti-exospore monoclonal antibody on microsporidial development in vitro.
The possible role of humoral antibodies in the immune response to microsporidiosis was studied using a novel anti-exospore monoclonal antibody (MAb) P5/H1 which recognised Encephalitozoon cuniculi. The effect of the P5/H1 MAb on microsporidial growth in vitro resulted in a reduction of the numbers of E. cuniculi spores in a Vero E6 cell-line culture. This reduction in the number of infected cells and the decrease of intracellular spores in infected cells was found when MAb P5/H1 was present in cultures, compared to cultures with an irrelevant isotype control MAb. Moreover, the presence of P5/H1 MAb increased the number of phagocytosed spores in macrophage cultures, and increased the activation of macrophages measured by nitrite oxide production. These results suggest a possible partial role of specific humoral antibodies in the protection against E. cuniculi infection.
Sak
B
B
Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic. casio@paru.cas.cz
Saková
K
K
Ditrich
O
O
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D009670:Encephalitozoon cuniculi / Q000276:immunology* / Q000502:physiology*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D033761:Spores, Protozoan / Q000276:immunology* / Q000502:physiology; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007371:Interferon-gamma