Effect of antioxidant probucol on cell-mediated LDL oxidation in vitro and in vivo.
We studied the effect of phenol antioxidant probucol on free radical oxidation of LDL isolated from blood plasma of healthy donors. Oxidation was induced by co-incubation of LDL with cultured peripheral blood monocyte-macrophages and human umbilical vein endothelial cells under conditions of ischemia-reperfusion. In addition, the effect of probucol therapy on oxidability of plasma LDL in CHD patients was examined. Probucol (0.1-10 microM) efficiently protected LDL from free radical oxidation in vitro and in vivo.
Bilenko
M V
MV
V. N. Orekhovich State Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Khil'chenko
A V
AV
Konovalova
G G
GG
Lankin
V Z
VZ
D000924:Anticholesteremic Agents / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D002478:Cells, Cultured; D003327:Coronary Disease / Q000188:drug therapy; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011341:Probucol / Q000378:metabolism* / Q000627:therapeutic use
D000924:Anticholesteremic Agents; D000975:Antioxidants; D008077:Lipoproteins, LDL; D011341:Probucol