[Preparation of NK-enriched LAK cells--their potential cytotoxic and ADCC activities].
We examined several culture methods to induce proliferation of natural killer (NK) cells from peripheral blood mononuclear cells (PBMC). In the presence of IL-2, a remarkable proliferation of NK cells was observed when PBMC were co-cultured with MMC-treated K562, which is known as a highly sensitive in vitro target cell for the NK assay. Addition of OK-432 or TNF-alpha and IL-1 beta also induced marked NK proliferation in a dose dependent manner. These NK-enriched lymphokine activated killer (LAK) cells showed highly cytotoxic activities against various MHC class I positive or negative tumor cells. They also showed potent ADCC activities against Herceptin-coated SK-BR-3, a HER2/neu positive breast cancer cell line. These results indicated that NK-enriched LAK cells are potent effector cells, and suggested novel therapeutic strategies for nonspecific immunotherapy as well as target immunotherapy in combination with anticancer antibodies, such as Herceptin.
Kobayashi
Yasunobu
Y
Cancer Immunotherapy Research Center, J.B. Therapeutics Inc.
Sudo
Toshimi
T
Matsushita
Norimasa
N
Nakao
Masanobu
M
Tanaka
Yoshinori
Y
Shimizu
Kouichi
K
Tanigawa
Keishi
K
Aruga
Atsushi
A
D000920:Antibody-Dependent Cell Cytotoxicity; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology* / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology; D007963:Leukocytes, Mononuclear / Q000166:cytology; D010844:Picibanil / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D010844:Picibanil