title: [Detection of COL1A1/PDGFB fusion transcripts in dermatofibroscoma protuberans by revers transcriptase-polymerase chain reaction using paraffin-embedded tissues].
abstract: OBJECTIVE
  To detect the COL1A1/PDGFB fusion transcripts and discuss its clinicopathological significance in dermatofibroscoma protuberans.
  
  
  METHODS
  Formalin fixed, paraffin-embedded tumor specimens from 12 patients with DFSP were reviewed by light microscope and the expression of COL1A1/PDGFB mRNA resulting from the reciprocal translocation t(17;22) (q22;q13.1) was detected by one-step revers transcriptase-polymerase chain reaction. The following tumor specimens were included as controls: 2 fibrosarcoma, 2 malignant fibrous histocytoma, 3 leiomyosarcoma, 1 dermarofibroma and 1 nerve shealth tumor.
  
  
  RESULTS
  The COL1A1/PDGFB fusion transcripts were detected in 8 (67%) of 12 samples from patients with DFSP. Nucleotide sequence analysis using the PCR products confirmed that different regions of the COL1A1 gene, respectively, were fused with of PDGFB gene. No COL1A1/PDGFB fusion transcripts were detected in the control tumors.
  
  
  CONCLUSIONS
  Detection of specific COL1A1/PDGFB fusion transcripts in DFSP will help to diagnose the nature of DFSP and research the mechanism of its molecular histogenesis.
authors: Department of Pathology, School of Medicine, Shihezi University, Shihezi 832002, China.
authors: Jiang-hua
authors: JH
authors: Yang
authors: Wen-hao
authors: WH
authors: Hu
authors: Feng
authors: F
authors: Li
authors: Tian-cai
authors: TC
authors: Lu
authors: Hong-an
authors: HA
authors: Li
authors: Bin
authors: B
authors: Chang
authors: Xue-bao
authors: XB
authors: Zhang
authors: Xiao-ming
authors: XM
authors: Zhou
authors: Hong-wei
authors: HW
authors: Dang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D024042:Collagen Type I / Q000235:genetics*; D018223:Dermatofibrosarcoma / Q000235:genetics*; D005260:Female; D020571:Genes, sis; D006801:Humans; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012878:Skin Neoplasms / Q000235:genetics*
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins