But1 and But2 proteins bind to Uba3, a catalytic subunit of E1 for neddylation, in fission yeast.
NEDD8/Rub1 is the most homologous protein to ubiquitin among the ubiquitin-like proteins, and it is covalently linked to target proteins via the C-terminal glycine residue in a manner analogous to ubiquitylation. However, the mechanism(s) involved in the regulation of the NEDD8 ligation pathway remains elusive. Using the two-hybrid system, we isolated novel genes from the Schizosaccharomyces pombe cDNA library whose products bind to Uba3, which is a catalytic protein for E1-like activity of the NEDD8 pathway. We designated these genes but1(+) and but2(+) (for proteins that bind to Uba three). But1 is a nuclear protein and its overexpression caused cell elongation, which is a common phenotype of the NEDD8 pathway defective mutant in S. pombe. Furthermore, overexpression of but1(+) in ned8-temperature sensitive mutant had a deleterious effect even under permissive temperatures. Our results suggest that But1 may have an inhibitory role in the NEDD8 pathway.
Yashiroda
Hideki
H
Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo 113-8613, Japan.
Tanaka
Keiji
K
D020134:Catalytic Domain; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry; D015723:Gene Library; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics; D020798:Two-Hybrid System Techniques; D014452:Ubiquitins / Q000737:chemistry* / Q000378:metabolism
C479858:But1 protein, S pombe; C479859:But2 protein, S pombe; D018076:DNA, Complementary; D005656:Fungal Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D014452:Ubiquitins; C491803:uba3 protein, S pombe; D049452:Green Fluorescent Proteins