Flow cytometric analysis of DNA, telomerase content and multi-gene expression in esophageal epithelial dysplasia.
OBJECTIVE
To investigate the alteration of molecular events and the early carcinogenesis mechanism of esophageal epithelial cells in the high incidence area of esophageal cancer.


METHODS
Esophageal epithelial cells of esophageal cancer patients were collected from the high incidence area in China. Content of DNA and telomerase as well as multi-gene expressions such as p53, p21 and cyclin D1 in esophageal precancer cells were quantitatively analysed by flow cytometry (FCM) with indirect immunofluorescence technique and DNA propidium iodide fluorescence staining.


RESULTS
FCM analysis results showed the DNA content increased significantly and the heteroploid rate was 87.9% in occurred carcinogenesis. P53 protein accumulation and ras p21 increase were seen in the early carcinogenesis of the esophagus. The positive rate of p53 and ras p21 was 100% (5/5, 4/4 respectively) in the cancer group. Telomerase and oncogene cyclin D1 were over- expressed in all of the cancer patients.


CONCLUSIONS
Increased DNA content and heteroploid rate, accumulation of p53 protein, and over-expression of p21, telomerase and cyclin D1 proteins were early molecular events during the development of esophageal cancer.
Zuo
Lian-Fu
LF
Hebei Provincial Tumor Institute, Shijiazhuang 050011, Hebei Province, China. zuolianfu@sina.com
Lin
Pei-Zhong
PZ
Qi
Fing-Ying
FY
Guo
Jian-Wen
JW
Liu
Jiang-Hui
JH
D002352:Carrier Proteins / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004847:Epithelial Cells / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D016601:RNA-Binding Proteins; D019098:Telomerase / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D002352:Carrier Proteins; D016601:RNA-Binding Proteins; C104342:TEP1 protein, human; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D004247:DNA; D019098:Telomerase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)