Identification of carbonyl sulfide and sulfur dioxide in porcine coronary artery by gas chromatography/mass spectrometry, possible relevance to EDHF.
Incubation of porcine coronary artery rings and cardiac muscle tissue in Krebs buffer followed by GC/MS analysis of the headspace gas revealed two gases, carbonyl sulfide (COS) and sulfur dioxide (SO(2)). The gases were identified by characteristic ions obtained by electron ionization, and by comparison of the retention time on a chromatographic column (GS GasPro) with standards of these gases. Stimulation of the arterial rings with acetylcholine and calcium ionophore A23187 increased the levels of SO(2) and COS in the vascular tissue. We also provide evidence that SO(2) could originate from disproportionation of a very unstable gas, sulfur monoxide (S=O). We suggest potential origins of these gases and discuss their relevance to endothelium-derived hyperpolarizing factor.
Balazy
Michael
M
Department of Pharmacology, New York Medical College, Valhalla, NY 10595-1612, USA. Michael_Balazy@nymc.edu
Abu-Yousef
Imad A
IA
Harpp
David N
DN
Park
Joonsoo
J
D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D003331:Coronary Vessels / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D009206:Myocardium / Q000378:metabolism; D013458:Sulfur Dioxide / Q000378:metabolism*; D013461:Sulfur Oxides / Q000378:metabolism*; D013552:Swine; D013696:Temperature; D013997:Time Factors
D001685:Biological Factors; D013461:Sulfur Oxides; C067410:endothelium-dependent hyperpolarization factor; D013458:Sulfur Dioxide; C010063:carbonyl sulfide