title: Inhibitory effect of curcumin on proliferation of K562 cells involves down-regulation of p210(bcr/abl) initiated Ras signal transduction pathway.
abstract: OBJECTIVE
  To investigate the effects of curcumin (Cur) on proliferation of K562 cells and the relationship between these effects and Ras signal transduction pathway activated by p210bcr/abl.
  
  
  METHODS
  K562 cell line was used as a p210bcr/abl-positive cell system and HL-60 cell line as a p210bcr/abl-negative control; etoposide (VP-16), which has no influence on p210bcr/abl and has resistance to K562 cells[1], was used as an anticancer drug control to compare with curcumin. MTT was used to determine the proliferative effects of drugs on K562 and HL-60 cells. Western blot and flow cytometry were used to examine the abundance of signal protein molecules expressed in tumor cells.
  
  
  RESULTS
  An exposure of K562 cells or HL-60 cells to Cur produced both concentration- and time-dependent increase in the anti-proliferative rate. Moreover, both cell lines had the same sensitivity to Cur (P>0.05). In contrast, HL-60 cells had more sensitivity to VP-16 than K562 cells in anti-proliferative effect (P<0.01). The abundance of p210bcr/abl as well as MEK-1 and c-JUN proteins were strongly down-regulated in curcumin-treated p210bcr/abl-positive K562 cells while c-JUN and MEK-1 proteins were only slightly down-regulated in p210bcr/abl-negative HL-60 cells.
  
  
  CONCLUSIONS
  Curcumin inhibited the proliferation of K562 cells and the inhibitory effect was correlated with down-regulation of the abundance of p210bcr/abl, which may ultimately lead to retard the Ras signal transduction pathway. Curcumin might be worthy of being evaluated as a potential chemotherapeutic agent to CML.
authors: Department of Pharmacology, Institute of Clinical Pharmacology, Fujian Medical University, Fuzhou 350004, China. jhx58@hotmail.com
authors: Li-Xian
authors: LX
authors: Wu
authors: Jian-Hua
authors: JH
authors: Xu
authors: Guo-Hua
authors: GH
authors: Wu
authors: Yuan-Zhong
authors: YZ
authors: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D011905:Genes, ras; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016044:Fusion Proteins, bcr-abl; D003474:Curcumin