title: Oral administration of beta-lactamase preserves colonization resistance of piperacillin-treated mice.
abstract: We hypothesized that orally administered, recombinant class A beta-lactamase would inactivate the portion of parenteral piperacillin excreted into the intestinal tract, preserving colonization resistance of mice against nosocomial pathogens. Subcutaneous piperacillin or piperacillin plus oral beta-lactamase were administered 24 and 12 h before orogastric inoculation of piperacillin-resistant pathogens. Oral administration of beta-lactamase reduced piperacillin-associated alteration of the indigenous microflora and prevented overgrowth of pathogens.
authors: Division of Infectious Diseases, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA. curtisd123@yahoo.com
authors: Usha
authors: U
authors: Stiefel
authors: Nicole J
authors: NJ
authors: Pultz
authors: Jaana
authors: J
authors: Harmoinen
authors: Pertti
authors: P
authors: Koski
authors: Kai
authors: K
authors: Lindevall
authors: Marion S
authors: MS
authors: Helfand
authors: Curtis J
authors: CJ
authors: Donskey
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism; D002177:Candidiasis / Q000188:drug therapy; D017714:Community-Acquired Infections / Q000188:drug therapy; D003428:Cross Infection / Q000188:drug therapy; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004357:Drug Synergism; D016984:Enterococcus faecium / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy; D007710:Klebsiella Infections / Q000188:drug therapy; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010878:Piperacillin / Q000493:pharmacokinetics / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D018440:beta-Lactam Resistance / Q000187:drug effects; D001618:beta-Lactamases / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004271:DNA, Fungal; D001618:beta-Lactamases; D010878:Piperacillin