Remineralization of demineralized albumin-calcium phosphate coatings.
Calcium phosphate and bovine serum albumin were coprecipitated (under physiological conditions of temperature and pH) upon the surfaces of titanium-alloy samples, which thereby became coated with a dense, proteinaceous mineral layer 30-50 microm in thickness. Dissolution of the inorganic phase by treatment with acidic saline yielded a self-supporting protein scaffold, 7-10 microm in thickness. Energy-dispersive X-ray analysis and Fourier-transform infrared spectroscopy confirmed the absence of inorganic components from the demineralized albumin scaffolds. When titanium-alloy samples bearing these demineralized protein scaffolds were immersed in a supersaturated solution of calcium phosphate (again at physiological temperature and pH), they remineralized. These redux albumin-calcium phosphate layers corresponded in thickness to the original coatings. When titanium-alloy discs bearing the demineralized protein scaffolds were implanted ectopically (subcutaneously) in mice, they, too, remineralized. No uniform mineral layer was deposited upon the surfaces of naked titanium-alloy implants. To the best of our knowledge, this is the first demonstration of remineralization within the interstices of a noncollagenous protein scaffold, either in vitro or in vivo.
Liu
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Y
Biomaterials Research Group, Leiden University, The Netherlands. maria.liu@isotis.com
Hunziker
E B
EB
Layrolle
P
P
Van Blitterswijk
C
C
Calvert
P D
PD
de Groot
K
K
D000497:Alloys / Q000737:chemistry; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry
D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D012710:Serum Albumin, Bovine; D014025:Titanium