title: Aryldiazomethanes for universal labeling of nucleic acids and analysis on DNA chips.
abstract: DNA and RNA labeling and detection are key steps in nucleic acid-based technologies, used in medical research and molecular diagnostics. We report here the synthesis, reactivity, and potential of a new type of labeling molecule, m-(N-Biotinoylamino)phenylmethyldiazomethane (m-BioPMDAM), that reacts selectively and efficiently with phosphates in nucleotide monomers, oligonucleotides, DNA, and RNA. This molecule contains a biotin as detectable unit and a diazomethyl function as reactive moiety. We demonstrate that this label fulfills the requirements of stability, solubility, reactivity, and selectivity for hybridization-based analysis and especially for detection on high-density DNA chips.
authors: BioMérieux, Advanced Technology/Molecular Diagnostics, Marcy l'Etoile, France. ali.laayoun@eu.biomerieux.com
authors: Ali
authors: A
authors: Laayoun
authors: Mitsuharu
authors: M
authors: Kotera
authors: Isabelle
authors: I
authors: Sothier
authors: Emmanuelle
authors: E
authors: Trévisiol
authors: Eloy
authors: E
authors: Bernal-Méndez
authors: Cécile
authors: C
authors: Bourget
authors: Lionel
authors: L
authors: Menou
authors: Jean
authors: J
authors: Lhomme
authors: Alain
authors: A
authors: Troesch
mesh_terms: D019921:Biotinylation; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D003978:Diazomethane / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009696:Nucleic Acids; D003978:Diazomethane; D012313:RNA; D004247:DNA