T Cell Integrin Overexpression as a Model of Murine Autoimmunity.
Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to investigate the role of T cell integrin adhesion molecules in the development of autoimmunity. This report summarizes the methods we used in establishing this murine model. By treating murine CD4+ T cells with DNA hypomethylating agents and by transfection we were able to test the in vitro effects of integrin overexpression on T cell autoreactive proliferation, cytotoxicity, adhesion and trafficking. Furthermore, we showed that the ability to induce in vivo autoimmunity may be unique to the integrin lymphocyte function associated antigen-1 (LFA-1).
Yung
Raymond L.
RL
Department of Internal Medicine, University of Michigan. Ann Arbor, MI, 48109-0940. USA.
Ray
Donna
D
Mo
Ru Ran
RR
Chen
Jun
J