Increased activity of glycerol 3-phosphate dehydrogenase and other lipogenic enzymes in human bladder cancer.
Common molecular changes in cancer cells are high carbon flux through the glycolytic pathway and overexpression of fatty acid synthase, a key lipogenic enzyme. Since glycerol 3-phosphate dehydrogenase creates a link between carbohydrates and the lipid metabolism, we have investigated the activity of glycerol 3-phosphate dehydrogenase and various lipogenic enzymes in human bladder cancer. The data presented in this paper indicate that glycerol 3-phosphate dehydrogenase activity in human bladder cancer is significantly higher compared to adjacent non-neoplastic tissue, serving as normal control bladder tissue. Increased glycerol 3-phosphate dehydrogenase activity is accompanied by increased enzyme activity, either directly (fatty acid synthase) or indirectly (through ATP-citrate lyase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and citrate synthase) involved in fatty acid synthesis. Coordinated upregulation of glycerol 3-phosphate dehydrogenase and lipogenic enzymes activities in human bladder cancer suggests that glycerol 3-phosphate dehydrogenase supplies glycerol 3-phosphate for lipid biosynthesis.
Turyn
J
J
Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland.
Schlichtholz
B
B
Dettlaff-Pokora
A
A
Presler
M
M
Goyke
E
E
Matuszewski
M
M
Kmieć
Z
Z
Krajka
K
K
Swierczynski
J
J
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000096:biosynthesis*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000201:enzymology*
D008055:Lipids; D005993:Glycerolphosphate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D064429:Fatty Acid Synthases; D002950:Citrate (si)-Synthase; D001275:ATP Citrate (pro-S)-Lyase