title: Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain.
abstract: OBJECTIVE
  To investigate the inhibition of p27kip1 gene on the growth of esophageal carcinoma cell strain (EC9706).
  
  
  METHODS
  Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effect on p27kip1 expression, the growth of esophageal carcinoma cell, DNA replication, protein synthesis, cell multiplication and apoptosis were explored by means of cell growth count, 3H-TdR, 3H-Leucine incorporation, flow cytometry, DNA fragment analysis and TUNEL.
  
  
  RESULTS
  Recombinant adenovirus Ad-p27kip1 was successfully constructed with a virus titer of 1.24 X 10(12) pfu/ml. p27kip protein expression increased markedly after EC-9706 transfection, while incorporation quantity of 3H-TdR and 3H-Leucine decreased significantly. The growth of esophageal carcinoma cell was inhibited obviously. Testing of flow cytometry displayed a typical apoptosis peak, and DNA gel electrophoresis showed a typical apoptosis ladder. TUNEL showed the apoptosis rate of Ad-p27kip1 group and control group to be 37.3% and 1.26% (P<0.001) respectively.
  
  
  CONCLUSIONS
  Ad-p27kip1 can inhibit the growth and multiplication of esophageal carcinoma cells and induce apoptosis. Therefore, enhanced p27kip1 expression may be a new way to treat esophageal carcinoma.
authors: Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, China. wuhe9224@sina.com
authors: Qing-Ming
authors: QM
authors: Wu
authors: Jie-Ping
authors: JP
authors: Yu
authors: Qiang
authors: Q
authors: Tong
authors: Xiao-Hu
authors: XH
authors: Wang
authors: Guo-Jian
authors: GJ
authors: Xie
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis; D018797:Cell Cycle Proteins / Q000235:genetics*; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor / Q000166:cytology / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004938:Esophageal Neoplasms; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020287:In Situ Nick-End Labeling; D007930:Leucine / Q000493:pharmacokinetics; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013936:Thymidine / Q000493:pharmacokinetics; D014162:Transfection; D014316:Tritium; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D014316:Tritium; D050760:Cyclin-Dependent Kinase Inhibitor p27; D007930:Leucine; D013936:Thymidine