title: Detection of Helicobacter pylori and its CagA gene in tonsil and adenoid tissues by PCR.
abstract: OBJECTIVE
  To determine the presence of Helicobacter pylori and, if detected, the prevalence of the CagA gene in adenotonsillectomy specimens by polymerase chain reaction (PCR).
  
  
  METHODS
  A prospective clinical trial.
  
  
  METHODS
  Tertiary referral center.
  
  
  METHODS
  The study population comprised 23 patients who had undergone adenoidectomy, tonsillectomy, or adenotonsillectomy under local or general anesthesia. Helicobacter pylori DNA was extracted from 3-mm-diameter tissue samples obtained from each tonsil and adenoid tissue specimens. The amplifications were performed for the 16S ribosomal RNA (rRNA) and CagA genes of H pylori in the samples of which H pylori DNA was detected.
  
  
  RESULTS
  In examining all the samples, 7 (30%) of 23 patients were shown to be positive for H pylori DNA, 5 (71%) of whom also possessed the CagA gene.
  
  
  CONCLUSIONS
  Tonsil and adenoid tissues may be an ecological niche of the mouth without regard to transient or permanent colonization. Oral-oral transmission may be a possible mode of spread of H pylori.
authors: School of Medicine, Department of Microbiology and Clinical Microbiology, Gazi University, Ankara, Turkey. meltemyc@gazi.edu.tr
authors: Meltem Yalinay
authors: MY
authors: Cirak
authors: Ali
authors: A
authors: Ozdek
authors: Dicle
authors: D
authors: Yilmaz
authors: Unal
authors: U
authors: Bayiz
authors: Erdal
authors: E
authors: Samim
authors: Sevgi
authors: S
authors: Turet
mesh_terms: D000234:Adenoids / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D014066:Palatine Tonsil / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori