title: Lack of cholecystokinin-A receptor enhanced gallstone formation: a study in CCK-A receptor gene knockout mice.
abstract: The etiology of gallstones is multifactorial, with interactions between genes and the environment. We generated cholecystokinin (CCK) -A receptor (R)-deficient (-/-) mice and found that CCK did not produce gallbladder contraction in CCK-AR(-/-) mice. The purpose of this study was to identify the role of CCK-AR on gallstone formation. Age-matched CCK-AR gene (+/+) and (-/-) progenies were used. Sludge and gallstone formation, as well as plasma cholesterol levels, were measured at 12 and 24 months of age. Sludge and gallstone formation were significantly higher in CCK-AR(-/-) mice than in CCK-AR(+/+) mice at 12 and 24 months of age, although these were not different between 12 and 24 months of age. The plasma cholesterol levels, daily food intake, and body weight were not significantly different between CCK-AR(+/+) and (-/-) mice. Sludge and gallstone formation were not observed at 6 months of age. In conclusion, deteriorated gallbladder contraction due to a lack of CCK-AR favored gallstone formation after the middle age of life.
authors: Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashiku, Tokyo-173-0015, Japan.
authors: Norikazu
authors: N
authors: Sato
authors: Kyoko
authors: K
authors: Miyasaka
authors: Shinji
authors: S
authors: Suzuki
authors: Setsuko
authors: S
authors: Kanai
authors: Minoru
authors: M
authors: Ohta
authors: Takako
authors: T
authors: Kawanami
authors: Yuki
authors: Y
authors: Yoshida
authors: Soichi
authors: S
authors: Takiguchi
authors: Tetsuo
authors: T
authors: Noda
authors: Yutaka
authors: Y
authors: Takata
authors: Akihiro
authors: A
authors: Funakoshi
mesh_terms: D000818:Animals; D005704:Gallbladder / Q000187:drug effects / Q000503:physiopathology; D042882:Gallstones / Q000209:etiology*; D051379:Mice; D018345:Mice, Knockout; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D043886:Receptor, Cholecystokinin A / Q000172:deficiency*; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: C090019:8-sulfocholecystokinin octapeptide; D043886:Receptor, Cholecystokinin A; D012844:Sincalide