title: A protein interaction map of Drosophila melanogaster.
abstract: Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.
authors: CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA.
authors: L
authors: L
authors: Giot
authors: J S
authors: JS
authors: Bader
authors: C
authors: C
authors: Brouwer
authors: A
authors: A
authors: Chaudhuri
authors: B
authors: B
authors: Kuang
authors: Y
authors: Y
authors: Li
authors: Y L
authors: YL
authors: Hao
authors: C E
authors: CE
authors: Ooi
authors: B
authors: B
authors: Godwin
authors: E
authors: E
authors: Vitols
authors: G
authors: G
authors: Vijayadamodar
authors: P
authors: P
authors: Pochart
authors: H
authors: H
authors: Machineni
authors: M
authors: M
authors: Welsh
authors: Y
authors: Y
authors: Kong
authors: B
authors: B
authors: Zerhusen
authors: R
authors: R
authors: Malcolm
authors: Z
authors: Z
authors: Varrone
authors: A
authors: A
authors: Collis
authors: M
authors: M
authors: Minto
authors: S
authors: S
authors: Burgess
authors: L
authors: L
authors: McDaniel
authors: E
authors: E
authors: Stimpson
authors: F
authors: F
authors: Spriggs
authors: J
authors: J
authors: Williams
authors: K
authors: K
authors: Neurath
authors: N
authors: N
authors: Ioime
authors: M
authors: M
authors: Agee
authors: E
authors: E
authors: Voss
authors: K
authors: K
authors: Furtak
authors: R
authors: R
authors: Renzulli
authors: N
authors: N
authors: Aanensen
authors: S
authors: S
authors: Carrolla
authors: E
authors: E
authors: Bickelhaupt
authors: Y
authors: Y
authors: Lazovatsky
authors: A
authors: A
authors: DaSilva
authors: J
authors: J
authors: Zhong
authors: C A
authors: CA
authors: Stanyon
authors: R L
authors: RL
authors: Finley
authors: K P
authors: KP
authors: White
authors: M
authors: M
authors: Braverman
authors: T
authors: T
authors: Jarvie
authors: S
authors: S
authors: Gold
authors: M
authors: M
authors: Leach
authors: J
authors: J
authors: Knight
authors: R A
authors: RA
authors: Shimkets
authors: M P
authors: MP
authors: McKenna
authors: J
authors: J
authors: Chant
authors: J M
authors: JM
authors: Rothberg
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism; D017344:Genes, Insect; D007113:Immunity, Innate; D008433:Mathematics; D015233:Models, Statistical; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020543:Proteome; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D020543:Proteome; D012333:RNA, Messenger; D066246:ErbB Receptors; D002118:Calcium