Affinity chromatography techniques based on the immobilisation of peptides exhibiting specific binding activity.
Affinity chromatography is one of the powerful techniques in selective purification and isolation of a great number of compounds. New challenges in scientific research, such as high-throughput systems, isolation procedures that allow to obtain a single substance from a complex matrix in high degree of purity, low costs and wide availability, have led to the discovery of new tailor-made synthetic recognition systems. In this review the design, synthesis, purification and characterisation of peptides with recognition properties are discussed. Applications of peptide ligands are described and analytical tools mentioned.
Tozzi
Cinzia
C
Department of Analytical Chemistry, University of Turin, Via P. Giuria 5, 10125 Turin, Italy. cinzia.tozzi@unito.it
Anfossi
Laura
L
Giraudi
Gianfranco
G
D002846:Chromatography, Affinity / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
D010455:Peptides