title: Fluorescent resolution target for super-resolution microscopy.
abstract: Historically, resolution in fluorescence optical microscopy has been limited by the Rayleigh criterion. Recently, however, several techniques have achieved resolution below that specified by the Rayleigh criterion. Among these are 4-Pi confocal microscopy, harmonic excitation light-microscopy, stimulated emission depletion microscopy, near-field scanning optical microscopy and I(5)M. The most widely accepted current method of resolution testing is to image an array of closely packed fluorescent beads or beads dispersed in a matrix. This shows that the system is capable of resolving a feature with a given diameter; however, it does not demonstrate the classical resolution of the system. We have fabricated a fluorescent resolution target for better characterization of a system's resolution.
authors: Harvard Medical School Technology and Engineering Center, Boston, MA, USA.
authors: P R H
authors: PR
authors: Stark
authors: L J
authors: LJ
authors: Rinko
authors: D N
authors: DN
authors: Larson
mesh_terms: D005456:Fluorescent Dyes; D005708:Gallium; D007089:Image Enhancement; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D019904:Polymethyl Methacrylate; D012235:Rhodamines
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate; D019904:Polymethyl Methacrylate; D005708:Gallium