Cloning and mutation analysis of ZFP276 as a candidate tumor suppressor in breast cancer.
Loss of heterozygosity (LOH) involving chromosome 16q23.4 occurs frequently in breast tumors, which suggests that this region may contain a tumor suppressor gene. Since ZFP276 is located in this region, we have therefore cloned and performed mutation analysis of its coding region in 70 breast tumors. One silent polymorphism and two alterations predicted to result in amino acid changes were detected. Absence of the wild-type allele in tumors carrying the E530D variant suggests a possible role for this change in tumorigenesis.
Wong
Jasmine C Y
JCY
Program in Genetics and Genomics Biology, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1X8, Canada. jcywong@sickkids.ca.|Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada. jcywong@sickkids.ca.
Gokgoz
Nalan
N
Fred A. Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada.
Alon
Noa
N
Program in Genetics and Genomics Biology, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1X8, Canada.
Andrulis
Irene L
IL
Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.|Fred A. Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada.
Buchwald
Manuel
M
Program in Genetics and Genomics Biology, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1X8, Canada.|Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.
D000483:Alleles; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D016335:Zinc Fingers
D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; C481818:ZNF276 protein, human