Aneugenic and clastogenic effects of doxorubicin in human lymphocytes.
Doxorubicin, a benzanthroquinone anticancer agent, was examined for its effect on micronucleus induction in cultured human lymphocytes. A statistically significant dose-dependent increase in micronucleus frequency (P<0.001) in binucleated cells was seen and an increase in the kinetochore-positive (P<0.001) and kinetochore-negative micronuclei (P<0.001) was observed. An increase was also observed in the number of necrotic cells, but the frequency of apoptotic cells remained almost constant. This confirms that doxorubicin is both clastogenic and aneugenic.
Dhawan
Alok
A
Industrial Toxicology Research Centre, PO Box-80, M.G.Marg, Lucknow 226 001, Uttar Pradesh, India.
Kayani
Mahmood A
MA
Parry
James M
JM
Parry
Elizabeth
E
Anderson
Diana
D
D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009336:Necrosis; D008941:Spindle Apparatus / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D004247:DNA