Molecular cloning and characterization of a novel human kinase gene, PDIK1L.
We isolated a 4301-bp cDNA from a human foetal brain cDNA library by high-throughput cDNA sequencing. It encodes a protein of 341 amino acids, which shows 69% identity with the human kinase CLIK1 (AAL99353), which was suggested to be the CLP-36 interacting kinase. Bioinformatics analysis suggests that the putative kinase may interact with PDZ and LIM domain proteins. Therefore the protein and its cDNA were named 'PDLIM1 interacting kinase 1 like' (PDIK1L; nomenclature approved by the HUGO Gene Nomenclature Committee). Ensembl Genome Browser located PDIK1L to human chromosome 1p35.3. It spans about 13.7 kb and consists of four exons and three introns. Multiple-tissue cDNA panel PCR revealed that the gene is expressed widely in human tissues: liver, kidney, pancreas, spleen, thymus and prostate. The protein appears to be localized to the nucleus.
Guo
Lingchen
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Ji
Chaoneng
C
Gu
Shaohua
S
Ying
Kang
K
Cheng
Haipeng
H
Ni
Xiaoghua
X
Liu
Jianping
J
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D060588:LIM Domain Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014018:Tissue Distribution; D014157:Transcription Factors
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C486312:LDB1 protein, human; D060588:LIM Domain Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C033652:postsynaptic density proteins; D010770:Phosphotransferases; D011494:Protein Kinases; C482688:PDIK1L protein, human; D017346:Protein Serine-Threonine Kinases; C470368:STK35 protein, human