Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.
A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
Nauwelaerts
Koen
K
Rega Institute for Medical Research, Laboratory for Medicinal Chemistry, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Vastmans
Karen
K
Froeyen
Matheus
M
Kempeneers
Veerle
V
Rozenski
Jef
J
Rosemeyer
Helmut
H
Van Aerschot
Arthur
A
Busson
Roger
R
Lacey
Jeffrey C
JC
Efimtseva
Ekaterina
E
Mikhailov
Sergey
S
Lescrinier
Eveline
E
Herdewijn
Piet
P
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004256:DNA Polymerase I / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D013379:Substrate Specificity; D013816:Thermodynamics
D004187:Disaccharides; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D010504:Periodic Acid; D012972:Sodium Hydroxide; D000255:Adenosine Triphosphate; D004247:DNA; C009288:metaperiodate; D004256:DNA Polymerase I