Carbon starvation survival of Listeria monocytogenes in planktonic state and in biofilm: a proteomic study.
The proteomes of Listeria monocytogenes expressed in suspension and biofilm state, in the presence and absence of a carbon source, were analysed by two-dimensional electrophoresis with the help of computer software. The up-regulated proteins in each case were identified by peptide sequencing using electrospray ionisation tandem mass spectrometry and a database search against the Listeria genome was performed. Relevant functions could be attributed to a number of the induced proteins which contribute to the understanding of the mechanisms of starvation survival of L. monocytogenes in planktonic state and in biofilm.
Helloin
Emmanuelle
E
Institut National de la Recherche Agronomique, Unité de Pathologie Infectieuse et Immunologie, Nouzilly, France.
Jänsch
Lothar
L
Phan-Thanh
Luu
L
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D002244:Carbon / Q000172:deficiency* / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000172:deficiency / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D007526:Isoelectric Point; D007553:Isotope Labeling; D008089:Listeria monocytogenes / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D013193:Stainless Steel; D015854:Up-Regulation / Q000187:drug effects
D001426:Bacterial Proteins; D020543:Proteome; D013193:Stainless Steel; D002244:Carbon; D005947:Glucose