Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC.
An HPLC method for the determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf has been developed. The separation system consisted of a C18 reversed-phase column, a gradient elution system of methanol/water and orthophosphoric acid, and a photodiode array detector. The concentrations of hamamelitannin, gallic acid, (+)-gallocatechin and (+)-catechin were 4.77, 0.59, 0.22 and 0.39% (w/w), respectively, in the bark. Hamamelitannin and catechins were also detected in the leaves at concentration less than 0.04% (w/w). This method is simple, sensitive and reproducible, ideally suited for rapid, routine analysis.
Wang
Huafu
H
R&D Department, William Ransom & Son plc, Hitchin, Herts SG5 1LY, UK. hwang@williamransom.com
Provan
Gordon J
GJ
Helliwell
Keith
K
D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D029501:Hamamelis; D006601:Hexoses / Q000032:analysis*; D024301:Plant Bark; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves; D018547:Plant Stems
D006601:Hexoses; D010936:Plant Extracts; D005707:Gallic Acid; D002392:Catechin; C093412:hamamelitannin