Effect of temperature on viability of Campylobacter jejuni and Campylobacter coli on raw chicken or pork skin.
To determine growth and survival of Campylobacter jejuni and Campylobacter coli on chicken and pork, Campylobacter spp. (10(4) CFU/cm2) were inoculated on pieces of raw, irradiated chicken or pork skin and exposed to temperatures ranging from -20 to 42 degrees C under either microaerobic or aerobic conditions. Viable counts over 48 h declined 2 to 3 log CFU/cm2 at -20 degrees C and 1 to 2 log CFU/cm2 at 25 degrees C regardless of skin type, species of Campylobacter, or level of oxygen. At 4 degrees C, there was no significant change in the number of Campylobacter over 48 h. At both 37 and 42 degrees C, the number of viable Campylobacter increased significantly (2 to 3 log CFU/cm2, P < 0.0001) under microaerobic conditions but decreased 0.5 to 1.5 log CFU/cm2 in air. Preincubation of skins for 24 h at 42 degrees C under microaerobic conditions to establish Campylobacter on the surface prior to lowering the temperature to -20, 4, or 25 degrees C and incubating in air resulted in a decline in viability for the first 4 h (0.5 to 1 log CFU/cm2). However, after this initial drop in viability, no additional effect on viability was observed compared with incubation at -20, 4, or 25 degrees C in air without microaerobic preincubation at 42 degrees C. Preincubation of inoculated skins at -20, 4, or 25 degrees C in air for 24 h followed by a shift in temperature to 42 degrees C for 4, 8, 24, or 48 h and a shift to microaerobic conditions resulted in an overall decline in viability on raw pork skin but not on raw chicken skin. In contrast, preincubation of inoculated skins at -20, 4, or 25 degrees C for 24 h in air followed by a shift in temperature to 37 degrees C and microaerobic conditions did not result in a decrease in viable counts for either chicken or pork skins. Overall, viability of C. coli and C. jejuni on chicken and pork skins was similar. Therefore, a lower incidence of Campylobacter spp. in pork than in poultry postslaughter, despite a similar prevalence in live animals, is not due to differences in viability of C. coli versus C. jejuni on raw chicken or pork skin.
Solow
Barbara Thurston
BT
U.S. Department of Agriculture Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.
Cloak
Orla M
OM
Fratamico
Pina M
PM
D000818:Animals; D017000:Campylobacter coli / Q000254:growth & development* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000254:growth & development* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D008855:Microscopy, Electron, Scanning; D012867:Skin / Q000382:microbiology* / Q000648:ultrastructure; D013552:Swine / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors