title: FAD transport and FAD-dependent protein thiol oxidation in rat liver microsomes.
abstract: The transport of FAD and its effect on disulfide bond formation was investigated in rat liver microsomal vesicles. By measuring the intravesicular FAD-accessible space, we observed that FAD permeates across the microsomal membrane and accumulates in the lumen. Rapid filtration experiments also demonstrated the uptake and efflux of the compound, which could be inhibited by atractyloside and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. FAD entering the lumen promoted the oxidation of protein thiols and increased the intraluminal oxidation of glucose-6-phosphate. These findings support the notion that, similar to yeast, free FAD may have a decisive role in the mechanism of oxidative protein folding in the endoplasmic reticulum lumen of mammalian cells.
authors: Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 1444 Budapest, Hungary.
authors: Marianne
authors: M
authors: Varsányi
authors: András
authors: A
authors: Szarka
authors: Eszter
authors: E
authors: Papp
authors: Dóra
authors: D
authors: Makai
authors: Gábor
authors: G
authors: Nardai
authors: Rosella
authors: R
authors: Fulceri
authors: Péter
authors: P
authors: Csermely
authors: József
authors: J
authors: Mandl
authors: Angelo
authors: A
authors: Benedetti
authors: Gábor
authors: G
authors: Bánhegyi
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001278:Atractyloside / Q000494:pharmacology; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds; D005182:Flavin-Adenine Dinucleotide; D001278:Atractyloside; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D010100:Oxygen