Less impaired cell-mediated immune response in the murine peritoneal cavity after CO(2) pneumoperitoneum.
OBJECTIVE
We compared changes in the populations of peritoneal T lymphocytes and natural killer (NK) cells after CO(2) pneumoperitoneum and laparotomy to clarify whether pneumoperitoneum affects cell-mediated immune responses in the peritoneal cavity.


METHODS
We analyzed and compared populations of T lymphocytes and NK cells among peritoneal exudative cells (PECs) collected from 185 female mice subjected to pneumoperitoneum, laparotomy, or anesthesia only. PECs were collected postoperatively, and the populations of T lymphocytes and NK cell subsets were analyzed by flow cytometry. The NK cell cytotoxicity (NKCC) of PECs and splenocytes was measured.


RESULTS
The populations of CD3(+), CD4(+), and CD8(+) lymphocytes in the PECs continued to increase up until postoperative day (POD) 7 after laparotomy. The CD4/8 ratio on POD 3 was significantly lower after laparotomy than after pneumoperitoneum. The percentages of NK cells in the pneumoperitoneum group were significantly lower than those in the laparotomy group. On POD 1, the NKCC of splenocytes was less impaired in the pneumoperitoneum group than in the laparotomy group (10.3% vs 5.0%, P << 0.05).


CONCLUSIONS
Laparoscopic surgery is preferable to open surgery because it results in less impairment of systemic and intraperitoneal cell-mediated immune responses.
Fujii
Kyuzo
K
First Department of Surgery, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, 879-5593 Oita, Japan.
Izumi
Koichi
K
Sonoda
Kazuya
K
Shiraishi
Norio
N
Adachi
Yosuke
Y
Kitano
Seigo
S
D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology; D010535:Laparoscopy / Q000009:adverse effects / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000601:surgery; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects / Q000379:methods*; D011336:Probability; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
D002245:Carbon Dioxide