Evaluation of methods for recovery of Salmonella from dairy cattle, poultry, and swine farms.
Current official methods for detection and isolation of Salmonella are mostly designed for foods. The objective of this study was to determine optimal methods for detection and isolation of Salmonella from animal and environmental samples of dairy, poultry, and swine farms. Preenrichment in lactose broth versus direct enrichment (no preenrichment) prior to selective enrichment in Rappaport-Vassiliadis, selenite cystine, and tetrathionate incubated at 35 and 42 degrees C and in four differential/selective plating media (brilliant green, bismuth sulfite, Hektoen enteric, and xylose-lysine-tergitol 4 agar base) were evaluated for their ability to recover Salmonella from artificially contaminated samples. The effects of pH adjustments to samples on Salmonella recovery were determined. A pH adjustment of the enrichment broth to 6.8 +/- 0.2 after addition of samples significantly improved recovery of Salmonella. The most effective medium combinations for isolation of Salmonella from farm samples depended on the type of samples. Generalizations of protocols for recovery of Salmonella from farm samples might result in poor recovery, increased recovery time, and increased sample processing costs.
Pangloli
Philipus
P
Food Safety Center of Excellence, Agricultural Experimental Station, The University of Tennessee, Knoxville, Tennessee 37996, USA.
Dje
Yobouet
Y
Oliver
S P
SP
Mathew
A
A
Golden
D A
DA
Taylor
W J
WJ
Draughon
F A
FA
D000821:Animal Feed / Q000382:microbiology; D000822:Animal Husbandry / Q000379:methods / Q000592:standards; D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle / Q000382:microbiology; D002480:Cellulase / Q000378:metabolism; D002645:Chickens / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D004783:Environmental Microbiology; D006197:Hair / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000382:microbiology; D012475:Salmonella / Q000302:isolation & purification*; D013552:Swine / Q000382:microbiology
D003470:Culture Media; D008372:Manure; D002480:Cellulase