title: A recombinant fragment of human SP-D reduces allergic responses in mice sensitized to house dust mite allergens.
abstract: C57Bl6 mice sensitized to Dermatophagoides pteronyssinus and challenged with D. pteronyssinus allergen extract given intranasally followed by treatment with intranasal applications of a 60-kDa truncated, trimeric recombinant form of human SP-D (rfhSP-D) showed a significant reduction in serum IgE, IgG1, peripheral blood eosinophilia and airway hyperresponsiveness compared to saline or bovine serum albumin-treated controls. Intracellular cytokine staining of lung and spleen homogenates showed increases in interleukin (IL)-12 production in lung tissue and normalization of IL-12 and interferon (IFN)-gamma in spleen tissue. In previous studies we demonstrated the effectiveness of native SP-D and rfhSP-D in down-regulating allergic responses to allergens of Aspergillus fumigatus. The results reported here indicate that rfhSP-D can suppress the development of allergic symptoms in sensitized mice challenged with allergens of the common house dust mite.
authors: Medical Research Council Immunochemistry Unit, Department of Biochemistry, University of Oxford, UK.
authors: P
authors: P
authors: Strong
authors: P
authors: P
authors: Townsend
authors: R
authors: R
authors: Mackay
authors: K B M
authors: KB
authors: Reid
authors: H W
authors: HW
authors: Clark
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004802:Eosinophilia / Q000517:prevention & control; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000276:immunology; D037663:Pulmonary Surfactant-Associated Protein D / Q000276:immunology*; D011663:Pulmonary Surfactants / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013154:Spleen / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D010446:Peptide Fragments; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D018664:Interleukin-12; D007073:Immunoglobulin E; D007371:Interferon-gamma