CbiX-homologous protein (CbiXhp), a metal-binding protein, from Streptomyces seoulensis is involved in expression of nickel-containing superoxide dismutase.
To find the accessory proteins participating in expression and maturation of nickel-containing superoxide dismutase (NiSOD), a metal-binding protein (CbiXhp) homologous to cobaltochelatase (CbiX) of Bacillus megaterium was isolated by nickel-nitrilotriacetic acid resin from Streptomyces seoulensis. The deduced amino acid sequence of cbiXhp showed 87% and 39% identity to CbiX of Streptomyces coelicolor and that of B. megaterium, respectively. Overexpression of CbiXhp increased the activity and the expression of NiSOD in the presence and absence of nickel, but to a lesser extent in its absence. This result indicates that CbiXhp is involved in the expression of NiSOD.
Kim
In-Kwon
IK
Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea.
Yim
Yang-In
YI
Kim
Young-Min
YM
Lee
Jin-Won
JW
Yim
Hyung-Soon
HS
Kang
Sa-Ouk
SO
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008190:Lyases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism*; D011485:Protein Binding; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D009532:Nickel; D013482:Superoxide Dismutase; D008190:Lyases; C033211:cobaltochelatase