title: [Detection of Trichomonas vaginalis with direct immunofluorescence assay].
abstract: OBJECTIVE
  To detect Trichomonas vaginalis (Tv) by direct immunofluorescence assay (DFA) and discuss its clinical significance.
  
  
  METHODS
  The parasites at a concentration of 3 x 10(5) cells/L were fixed by acetone on slides which were then blocked by 1% BSA (bovine serum albumin) or 10% BSA or 10% NCS (newborn calf serum) respectively, incubated with different dilution of polyclonal goat anti-Tv IgG (1:20-1:2,560) for different incubation time (15, 30, 45, 60, 90, 120 min). 120 clinical vaginal specimens were examined by direct immunofluorescence assay, the wet mount method and the in vitro cultivation.
  
  
  RESULTS
  Blocked by 1% or 10% BSA, incubated at 37 degrees C for 45 min with a titer 1:160 of polyclonal antibody were the optimal conditions for direct immunofluorescence assay. Its sensitivity and specificity were 87.9% and 98.6% respectively in comparison with the in vitro cultivation method.
  
  
  CONCLUSIONS
  Direct immunofluorescence assay is a useful alternative to the wet mount method which shows a lower sensitivity.
authors: Institute of Family Planning, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030.
authors: Yong-hong
authors: YH
authors: Tian
authors: Cheng-liang
authors: CL
authors: Xiong
authors: Huang-tao
authors: HT
authors: Guan
authors: Xue-bing
authors: XB
authors: Pang
authors: Chang-fu
authors: CF
authors: Jiang
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006041:Goats; D006801:Humans; D012680:Sensitivity and Specificity; D014247:Trichomonas Vaginitis / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000276:immunology / Q000302:isolation & purification*; D014621:Vagina / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan