Oral administration of beta-lactamase preserves colonization resistance of piperacillin-treated mice.
We hypothesized that orally administered, recombinant class A beta-lactamase would inactivate the portion of parenteral piperacillin excreted into the intestinal tract, preserving colonization resistance of mice against nosocomial pathogens. Subcutaneous piperacillin or piperacillin plus oral beta-lactamase were administered 24 and 12 h before orogastric inoculation of piperacillin-resistant pathogens. Oral administration of beta-lactamase reduced piperacillin-associated alteration of the indigenous microflora and prevented overgrowth of pathogens.
Stiefel
Usha
U
Division of Infectious Diseases, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA. curtisd123@yahoo.com
Pultz
Nicole J
NJ
Harmoinen
Jaana
J
Koski
Pertti
P
Lindevall
Kai
K
Helfand
Marion S
MS
Donskey
Curtis J
CJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism; D002177:Candidiasis / Q000188:drug therapy; D017714:Community-Acquired Infections / Q000188:drug therapy; D003428:Cross Infection / Q000188:drug therapy; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004357:Drug Synergism; D016984:Enterococcus faecium / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy; D007710:Klebsiella Infections / Q000188:drug therapy; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010878:Piperacillin / Q000493:pharmacokinetics / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D018440:beta-Lactam Resistance / Q000187:drug effects; D001618:beta-Lactamases / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004271:DNA, Fungal; D001618:beta-Lactamases; D010878:Piperacillin