title: Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol.
abstract: 3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
authors: Idaho National Engineering and Environmental Laboratory, PO Box 1625, MS-2203, Idaho Falls, ID 83415, USA.
authors: Mary E
authors: ME
authors: Kauffman
authors: William K
authors: WK
authors: Keener
authors: Scott R
authors: SR
authors: Clingenpeel
authors: Mary E
authors: ME
authors: Watwood
authors: David W
authors: DW
authors: Reed
authors: Yoshiko
authors: Y
authors: Fujita
authors: R Michael
authors: RM
authors: Lehman
mesh_terms: D000114:Acetylene / Q000031:analogs & derivatives* / Q000378:metabolism; D016956:Burkholderia cepacia / Q000201:enzymology / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D043367:Ralstonia / Q000201:enzymology / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
chemical_list: D002396:Catechols; D005456:Fluorescent Dyes; C031136:3-methylcatechol; D014050:Toluene; D000114:Acetylene