title: Analysis of aristolochic acid in nine sources of Xixin, a traditional Chinese medicine, by liquid chromatography/atmospheric pressure chemical ionization/tandem mass spectrometry.
abstract: Aristolochic acid I (AA-I), which is a known nephrotoxin, is found in a commonly used Chinese medicine, Xixin, that originates from nine Asarum species (Aristolochiaceae) found in China. A method has been developed using reversed-phase liquid chromatography coupled with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry under the positive ion detection mode [LC/(+)APCI/MS/MS] to determine the amount of AA-I in Xixin. The limit of detection of AA-I, estimated by monitoring with LC/MS/MS, was at the low microg/l level. By applying this method to methanol extracts of nine Asarum species, the concentrations of AA-I were found to range from 3.3 ng/mg (Asarum sieboldii) to 3376.9 ng/mg (Asarum crispulatum).
authors: Department of Chemistry, National Chung-Hsin University, Taichung, Taiwan. ttjong@mail.nchu.edu.tw
authors: Ting-Ting
authors: TT
authors: Jong
authors: Maw-Rong
authors: MR
authors: Lee
authors: Shun-Sheng
authors: SS
authors: Hsiao
authors: Jar-Lung
authors: JL
authors: Hsai
authors: Tian-Shung
authors: TS
authors: Wu
authors: Shu Tuan
authors: ST
authors: Chiang
authors: Shao-Qing
authors: SQ
authors: Cai
mesh_terms: D034341:Aristolochic Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D008516:Medicine, Chinese Traditional / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; C000228:aristolochic acid I