LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast.
Previously, we reported that the LAMMER kinase homolog, Lkh1, is a negative regulator of filamentous growth and asexual flocculation in the fission yeast, Schizosaccharomyces pombe. Here, we report that the lkh1(+) null mutant is sensitive to oxidative stress because of a reduction in the expression of genes for antioxidant enzymes such as catalase (ctt1(+)) and Cu,Zn-superoxide dismutase (sod1(+)). Furthermore, the lkh1(+) null mutant shows increased levels of intracellular peroxides under conditions of oxidative stress compared with wild-type cells. Interestingly, expression of the gene for the transcription factor Atf1 is reduced in the lkh1(+) null mutant under oxidative stress, whereas expression of the transcription factor Pap1 is not. We report the novel finding that Lkh1 is involved in the oxidative-stress response of the fission yeast, S. pombe, and regulates the expression of antioxidant enzymes via the transcription factor Atf1.
Park
Yoon-Dong
YD
Department of Microbiology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
Kang
Won-Hwa
WH
Yang
Wan-Soo
WS
Shin
Kwang-Soo
KS
Sook Bae
Kyung
K
Park
Hee-Moon
HM
D051697:Activating Transcription Factor 1; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D000073718:Pancreatitis-Associated Proteins; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D051697:Activating Transcription Factor 1; D004268:DNA-Binding Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D011494:Protein Kinases; C446922:lkh1 protein, S pombe