[The effect of liposome-carried metallothionein on secondary venous ischemia-reperfusion injury in a rat flap].
OBJECTIVE
To investigate the effect of liposome-carried metallothionein (lipo-MT) on secondary ischemia-reperfusion injury in the rat island flap.


METHODS
An abdominal island flap was created in the Wistar rat. The animals were divided into four groups: the sham group, the secondary ischemia-reperfusion group, the group treated with blank liposome and the group treated with lipo-MT. The malondialdehyde (MDA) content, the myeloperoxidase (MPO) activity was assayed immediately, at 30 minutes and 7 days after the secondary venous ischemia-reperfusion. The level of endothelin (ET) and lactic dehydrogenase (LDH) of the rat plasma was measured at 30 minutes after secondary venous ischemia-reperfusion. The content of MT of the flap was assayed by Cd-hemoglobin saturation method at 7 days after the operation.


RESULTS
The treatment of lipo-MT significantly decreased the content of MDA, MPO of the flap, decreased the activity of ET, LDH of the rat palsma, increased the content of MT of the flap and improved the flap viability.


CONCLUSIONS
Lipo-MT can improve flap survival by reducing ischemia-reperfusion injury.
Guo
Jie
J
Department of Plastic Surgery, Second Hospital, Harbin University, Harbin 150086, China.
Wang
Dong-yan
DY
Guan
De-hong
DH
Tang
Chao-shu
CS
D000818:Animals; D016232:Endothelins / Q000097:blood; D006085:Graft Survival; D007511:Ischemia / Q000378:metabolism; D045022:Lactate Dehydrogenases / Q000097:blood; D008081:Liposomes; D008315:Malondialdehyde / Q000378:metabolism; D008668:Metallothionein / Q000008:administration & dosage*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D013524:Surgical Flaps / Q000098:blood supply*; D013997:Time Factors
D016232:Endothelins; D008081:Liposomes; D008315:Malondialdehyde; D008668:Metallothionein; D045022:Lactate Dehydrogenases; D009195:Peroxidase