Neospora caninum infection and congenital transmission: serological and parasitological study of cows up to the fourth gestation.
In this paper, a parasitological and serological study performed in three cows up to the fourth gestation is reported in order to clarify the extent of vertical propagation and, secondly, in which period of gestation the recrudescence of previous infection occurs. The cows selected for the study delivered healthy but congenitally infected calves in first pregnancy. The parasite was found, by biological tests in Swiss mice, in all the placentas of the three cows examined, during the three subsequent gestations, at calving. The parasite was found, at slaughtering, in the brains of all nine calves born clinically healthy from the three cows as well. The serological profile, performed at monthly intervals on serum of the cows, showed that IgG and IgM increased in the third trimester of gestation; this rise of antibodies was constantly observed during the three gestations and in all three cows. In the calves, the IgG titres increase after colostrum consumption and an IgM peak at birth, were indicative of a late infection. These findings, along with negative results obtained by a serological study conducted simultaneously on 38 cows housed in the same stable as the experimental animals and the negative results obtained in isolating parasite or antibodies from farm dogs, suggest that N. caninum infection can be maintained over several bovine generations and that recrudescing persistent infection, rather than a new infection, explains the Neospora infection of calves.
Piergili Fioretti
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Department of Veterinary Biopathological Science, Faculty of Veterinary Medicine, University of Perugia, Via S. Costanzo, 4-06100 Perugia, Italy. dpf@unipg.it
Pasquali
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Diaferia
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Mangili
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Rosignoli
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D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000151:congenital / Q000469:parasitology / Q000635:transmission*; D003048:Coccidiosis / Q000635:transmission / Q000662:veterinary*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006321:Heart / Q000469:parasitology; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D008168:Lung / Q000469:parasitology; D018688:Neospora / Q000276:immunology* / Q000302:isolation & purification; D010298:Parity; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M