Survivin expression in atypical adenomatous hyperplasia of the lung.
Few studies have investigated apoptosis-related factors in atypical adenomatous hyperplasia (AAH) and nonmucinous bronchioloalveolar carcinoma. We studied the expression of survivin, bcl-2, and p53 in 29 AAH lesions (low-grade, 11; high-grade, 18) and 40 nonmucinous BACs using immunohistochemical analysis and of survivin messenger RNA in 6 nonmucinous BACs using reverse transcription-polymerase chain reaction (RT-PCR). The incidence of positive survivin expression was 9% (1/11) in low-grade AAH, 89% (16/18) in high-grade AAH, and 100% (40/40) in nonmucinous BAC. Statistically significant differences were found between low-grade and high-grade AAH and between high-grade AAH and nonmucinous BAC. The percentages obtained for positive bcl-2 and p53 expression were 18% (2/11) and 0% (0/11) in low-grade AAH, respectively, and 28% (5/18) for both in high-grade AAH and 48% (19/40) for both in nonmucinous BAC. In RT-PCR, the intensity of survivin messenger RNA expression was stronger in nonmucinous BACs than in normal lung tissue samples. Thus, the expression of the 3 antibodies in high-grade AAH was intermediate between low-grade AAH and nonmucinous BAC. High-grade AAH may be closely related to nonmucinous BAC.
Nakanishi
Kuniaki
K
Department of Pathology, National Defense Medical College, Tokorozawa, 359-8513, Japan.
Kawai
Toshiaki
T
Kumaki
Fumiyuki
F
Hiroi
Sadayuki
S
Mukai
Makio
M
Ikeda
Eiji
E
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000737:chemistry* / Q000473:pathology; D000236:Adenoma / Q000737:chemistry* / Q000473:pathology; D000368:Aged; D005260:Female; D006801:Humans; D006965:Hyperplasia; D051034:Inhibitor of Apoptosis Proteins; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D009363:Neoplasm Proteins; D011230:Precancerous Conditions / Q000737:chemistry* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D000077022:Survivin; D016159:Tumor Suppressor Protein p53