Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction.
IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and flt3 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells.
Kawamura
Toshihiko
T
Department of Pathology, University of Chicago, Chicago, IL 60637, USA.
Koka
Rima
R
Ma
Averil
A
Kumar
Vinay
V
D000818:Animals; D000950:Antigens, Ly / Q000096:biosynthesis* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019409:Interleukin-15 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D037181:Lectins, C-Type; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D021122:Protein Subunits / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011828:Radiation Chimera / Q000235:genetics / Q000276:immunology; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D053647:Receptors, Interleukin-15; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D055630:Receptors, NK Cell Lectin-Like
D000950:Antigens, Ly; C508025:Il15ra protein, mouse; D019409:Interleukin-15; D037181:Lectins, C-Type; D021122:Protein Subunits; D011971:Receptors, Immunologic; D053647:Receptors, Interleukin-15; D015375:Receptors, Interleukin-2; D055630:Receptors, NK Cell Lectin-Like