Usefulness of 31P-MRS as a method of evaluating the viability of preserved and transplanted rat liver.
We used phosphorus-31 magnetic resonance spectroscopy (31P-MRS) to evaluate the viability of transplanted rat liver. Wistar rats were used as donors and recipients. The donor livers were preserved in saline (group 1), Euro-Collins solution (group 2), or in University of Wisconsin (UW) solution (group 3) for 3 and 6 h in groups 1, 2 and 3 and for 9 h in groups 2 and 3. Thereafter the livers were orthotopically transplanted. 31P-MRS spectra were measured after portal reperfusion. Finally, all the recipients were divided into survivors and non-survivors. Survival rates were better in group 3 than in groups 1 and 2. In the 9-h-preserved livers, the livers in group 3 showed a significantly higher beta-ATP/Pi ratio than those in group 2. Comparing survivors and non-survivors in the 6-h-preserved livers in group 2, survivors' livers showed significantly higher beta-ATP/Pi ratio than those of non-survivors. We concluded that 31P-MRS is a useful method for assessing viability of rat liver grafts.
Kanetsuna
Y
Y
3rd Department of Surgery, Tokyo Women's Medical College, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, Japan.
Fujita
S
S
Tojimbara
T
T
Fuchinoue
S
S
Teraoka
S
S
Ota
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002314:Cardioplegic Solutions; D006085:Graft Survival / Q000502:physiology*; D006982:Hypertonic Solutions; D016031:Liver Transplantation / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D023421:Models, Animal; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D011652:Pulmonary Circulation; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000379:methods; D014185:Transplantation, Isogeneic / Q000502:physiology
D002314:Cardioplegic Solutions; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D010710:Phosphates; D010758:Phosphorus; D000255:Adenosine Triphosphate