cDNA cloning and characterization of a cotton peptide methionine sulfoxide reductase (cMsrA).
A full-length cDNA clone with high homology with Brassica napus peptide methionine sulfoxide reductase (PMSR) gene (BnPMSR), which could reverse oxidation of methionine residues into MetSO in protein, was cloned by suppression subtraction hybridization and cDNA library screening from Gossypium barbadense. This gene (named as cMsrA; Accession number: AY224208) had a total length of 1059 bp with an open reading frame of 765 bp, and encoded a predicted polypeptide of 255 amino acids with a molecular weight of 28.4 kDa. The cMsrA protein shared 68.6, 66.3 and 65.8% identity with those PMSR proteins isolated from B. napus, A. thaliana and L. sativa respectively. Expression patterns revealed that cMsrA was enriched later and weaker in resistant variety 7124 (Gossypium barbadense) than sensitive variety Ejing-1 (G. hirsutum) under salt treatment and pathogens attack. These results indicated that cMsrA played an important role in protecting the cells against oxidative damage during the pathogens and salt stress.
Zhao
Jingya
J
Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Zuo
Kaijing
K
Wang
Jin
J
Cao
Youfang
Y
Zhang
Lida
L
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D015870:Gene Expression; D015723:Gene Library; D003368:Gossypium / Q000235:genetics*; D051150:Methionine Sulfoxide Reductases; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D017931:DNA Primers; D012965:Sodium Chloride; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase