title: Effect of melatonin on cholestatic oxidative stress under constant light exposure.
abstract: This study was designed to evaluate the effect of melatonin on cholestatic oxidative stress under constant light exposure. Cholestasis was induced by double ligature and section of the extra-hepatic bile duct. Melatonin was injected i.p.(1000 microg kg(-1) day(-1)). Malondialdehyde, reduced glutathione, catalase, superoxide dismutase, glutathione reductase, peroxidase and transferase were determined in liver. After bile-duct obstruction and under constant light exposure, an increase in malondialdehyde (p < 0.05) and a slight decrease in reduced glutathione were seen. Enzyme activity, with the exception of glutathione reductase, had significantly diminished. After melatonin administration, malondialdehyde fell (p < 0.001), whereas there was an increase in reduced glutathione (p < 0.0001) compared with untreated controls. Constant light exposure was associated with an increase in hepatic oxidative stress. Treatment with melatonin decreased lipid peroxide synthesis, and permitted a recovery of both reduced glutathione and scavenger enzyme activity.
authors: Department of General Surgery, Reina Sofía University Hospital, Córdoba, Spain.
authors: Adolfo
authors: A
authors: Cruz
authors: Francisco J
authors: FJ
authors: Padillo
authors: Jose
authors: J
authors: Granados
authors: Isaac
authors: I
authors: Túnez
authors: M Carmen
authors: MC
authors: Muñoz
authors: Javier
authors: J
authors: Briceño
authors: Carlos
authors: C
authors: Pera-Madrazo
authors: Pedro
authors: P
authors: Montilla
mesh_terms: D000818:Animals; D002779:Cholestasis / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D008027:Light; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008315:Malondialdehyde; D005978:Glutathione; D008550:Melatonin