[Co-expression of CFA/I and CS6 of Enterotoxigenic Escherichia coli ( ETEC ) in Shigella flexneri 2a T32 Derivative Strain FWL01].
Among the known colonization factors of enterotoxigenic Escherichia coli (ETEC), CFA/I and CS6 (the common antigen in the CFA/IV fimbrial antigens ) are two of the most prevalent fimbriae found in clinical isolates but are never expressed by the same wild-type strains. In this study, CFA/I and CS6 of ETEC were co-expressed in Shigella flexneri 2a T32 derivative strain FWL01 by using a host-plasmid lethal balancing system based on asd gene. The results indicate that the recombinant plasmid carrying CFA/I and CS6 could be stably integrated in FWL01. Expression of the two antigens did not interfere the host growth. The results of immunofluorescence analysis showed that CFA/I and CS6 were localized on the surface of the strain FWL01. In Balb/c mice orally immunized with the recombinant strain, the immune responses against CFA/I and CS6 were observed. Those observations show the feasibility of a multivalent vaccine expressing different fimbrial antigens in attenuated Shigella flexneri.
Zheng
Ji-Ping
JP
Beijing Institute of Biotechnology, Beijing 100071, China.
Wang
Ling-Chun
LC
Wang
Peng
P
Luo
Gang
G
Li
Shu-Qin
SQ
Duan
Hai-Qing
HQ
Huang
Cui-Fen
CF
Zhang
Zhao-Shan
ZS
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012763:Shigella flexneri / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014613:Vaccines, Attenuated / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C098406:CS6 antigen, E coli; D029968:Escherichia coli Proteins; D014613:Vaccines, Attenuated; C022924:colonization factor antigens; D036781:Fimbriae Proteins