9p21 and 13q14 dosages in ependymomas. A clinicopathologic study of 101 cases.
Ependymomas are glial neoplasms whose clinical behavior is difficult to predict based on histology alone. Recently, a comparative genomic hybridization study identified frequent chromosome 9p and 13q losses in anaplastic ependymomas, suggesting that p16 and RB alterations may be involved in tumor progression. In order to test this hypothesis further, 101 myxopapillary, conventional, and anaplastic ependymomas (51 spinal and 50 intracranial tumors) were tested for RB and p16 deletions using fluorescence in situ hybridization. Clinical follow-up, ranging from 2 to 198 months (median 46 months), was obtained in 90 cases (91%). RB and p16 deletions were seen in 22 of 92 (24%) and 22 of 89 (25%) informative cases, respectively. Polysomies were more frequent in the grade I and II spinal tumors, consistent with prior reports of increased aneuploidy in such cases. No significant genetic associations were seen with tumor grade, recurrence, or death, suggesting that 9p and 13q deletions do not play a prominent role in the malignant progression of ependymomas, as has been implicated in other glioma subtypes.
Rajaram
Veena
V
Department of Pathology, Washington University School of Medicine, St Louis, MO 63110-1093, USA.
Leuthardt
Eric C
EC
Singh
Pratima K
PK
Ojemann
Jeffrey G
JG
Brat
Daniel J
DJ
Prayson
Richard A
RA
Perry
Arie
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D002882:Chromosomes, Human, Pair 13; D002899:Chromosomes, Human, Pair 9; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D004806:Ependymoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D005500:Follow-Up Studies; D017353:Gene Deletion; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008875:Middle Aged; D009367:Neoplasm Staging; D016160:Retinoblastoma Protein / Q000235:genetics; D013120:Spinal Cord Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D016896:Treatment Outcome
D019941:Cyclin-Dependent Kinase Inhibitor p16; D016160:Retinoblastoma Protein