title: Lack of correlation between telomere length and telomerase activity and expression in leukemic cells.
abstract: The expression of three components of telomerase complex (hTR, hTERT, TP1) along with telomerase activity and telomere length in leukemic cells was investigated. Cells were isolated from peripheral blood and/or bone marrow of children with acute lymphoblastic (ALL) and non-lymphoblastic (ANLL) leukemia. Expression of three components of telomerase as well as telomerase activity was found in all leukemic cells. Chemiluminescent detection of terminal restriction fragments (TRF) from DNA isolated from ALL cells showed variable patterns expressing considerable heterogeneity of telomere length. The ALL cells appeared to have both long and short telomere lengths, in contrast to normal peripheral lymphocytes, which produced limited pattern of TRF. The ANLL cells produced predominantly short telomere pattern despite high telomerase activity and expression. It can be concluded that high telomerase activity and expression in leukemic cells is not always correlated with long telomeres (TRF pattern).
authors: Institute of Human Genetics PAN, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors: Danuta
authors: D
authors: Januszkiewicz
authors: Jacek
authors: J
authors: Wysoki
authors: Krzysztof
authors: K
authors: Lewandowski
authors: Monika
authors: M
authors: Pernak
authors: Karina
authors: K
authors: Nowicka
authors: Jolanta
authors: J
authors: Rembowska
authors: Jerzy
authors: J
authors: Nowak
mesh_terms: D002874:Chromosome Mapping; D006801:Humans; D007938:Leukemia / Q000201:enzymology* / Q000235:genetics*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*
chemical_list: D019098:Telomerase