Investigations on the influence of helminth parasites on vaccination of chickens against Newcastle disease virus under village conditions.
Prevalence studies have shown that almost 100% of free-range chickens are infected with a wide range of parasites. The infections are mostly subclinical in nature, resulting in production losses and occasionally mortality. Newcastle disease (ND) on the other hand, results in high mortality rates during epidemics. ND is a limiting factor for increasing poultry production in many tropical countries, where frequent reports indicate vaccination failures. The aim of our study was to investigate the influence of helminths on the antibody response after vaccination against Newcastle disease of free-range chickens naturally infected with parasites. Sixty chickens were divided into six groups, of which three were vaccinated against ND with a live De Soto vaccine, while the other three remained non-vaccinated. One group within the vaccinated groups and the one within the non-vaccinated group was kept naturally infected with helminth parasites, while the other two groups in each set were dewormed with fenbendazole and niclosamide, and one of each of these groups was subsequently infected with Ascaridia galli. After vaccination, all the groups were followed for 5 weeks and their antibody titres were determined weekly using a HI test. All the birds were finally challenged 4 weeks after vaccination with a virulent velogenic ND virus obtained from a field outbreak. All the vaccinated chickens seroconverted and had high antibody levels after 3 weeks, but these dropped to low levels at 4 weeks after vaccination. After challenge, the antibody titres rose in the dewormed groups but not in the parasite-infected groups. After 5 weeks, all the parasite-infected animals had significantly lower antibody titres than the dewormed animals. All the vaccinated chickens survived the challenge infection, emphasizing the importance of the cellular immune response. Further studies are needed to examine the effects of the parasitic infection on protection against ND over a longer period.
Hørning
G
G
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Dyrlagevej 2, DK-1870 Frederiksberg C, Denmark.
Rasmussen
S
S
Permin
A
A
Bisgaard
M
M
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001197:Ascaridia / Q000472:pathogenicity; D002645:Chickens / Q000276:immunology* / Q000469:parasitology* / Q000821:virology; D006374:Helminthiasis, Animal / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*; D015030:Zimbabwe
D000914:Antibodies, Viral