Acute effects of PYY3-36 on food intake and hypothalamic neuropeptide expression in the mouse.
It has recently been suggested that gut-derived PYY(3-36) may be involved in the central mediation of post-prandial satiety signals. We have examined the acute effects of peripherally administered PYY(3-36) on food intake and hypothalamic gene expression of neuropeptides in mice. A single intraperitoneal injection of PYY(3-36) to mice that had been fasted for 24h resulted in a highly significant reduction in food intake at 6 and 24h post-injection but not at 48h. However, in freely fed mice, food intake was unaltered by PYY(3-36) administration. In the arcuate nucleus POMC mRNA expression was significantly elevated at 6h and remained elevated at 24h following PYY(3-36) injection. By contrast NPY mRNA expression in the arcuate nucleus was suppressed at 6h but not at 24h post-injection. In the lateral hypothalamus there were no differences in MCH mRNA expression at either time point. In conclusion, peripherally administered PYY(3-36) has a suppressive effect on food intake that is more prominent in recently fasted mice and lasts up to 24 h. This is associated with a short-lived suppression of NPY mRNA, a longer lasting increase in POMC mRNA but no change in MCH mRNA expression.
Challis
B G
BG
Department of Medicine and Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, CB2 2QQ, Cambridge, UK.
Pinnock
S B
SB
Coll
A P
AP
Carter
R N
RN
Dickson
S L
SL
O'Rahilly
S
S
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D007028:Hypothalamic Hormones / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008543:Melanins / Q000378:metabolism; D051379:Mice; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000008:administration & dosage*; D019894:Peptide YY / Q000008:administration & dosage*; D010907:Pituitary Hormones / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000378:metabolism
D007028:Hypothalamic Hormones; D008543:Melanins; D009478:Neuropeptide Y; D009479:Neuropeptides; D010446:Peptide Fragments; D010907:Pituitary Hormones; C078948:peptide YY (13-36); D019894:Peptide YY; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone