Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes.
Protection against Schistosoma mansoni infection in C57BL/6 female mice was evaluated by two DNA vaccination strategies. Mice were either vaccinated by intramuscular injection with pcDNAI/Amp constructs encoding either Cu/Zn cytosolic superoxide dismutase (CT-SOD), signal peptide-containing SOD (SP-SOD), glutathione peroxidase (GPX(bb)) or a mutated form of GPX (GPX(m)), or primed with naked DNA constructs and boosted with recombinant vaccinia virus (RVV) containing the same genes. Animals were then challenged with S. mansoni and the level of protection was assessed as the reduction in worm burden. CT-SOD showed significant levels of protection compared to the control group, ranging between 44 and 60%, while SP-SOD exhibited from 22 to 45%. GPX(bb) showed levels of protection (23-55%) higher than GPX(m) (25-34%). The prime-boost strategy gave the same results as naked DNA or recombinant vaccinia virus alone except in the case of GPX, where the protection was 85%.
Shalaby
Kamal A
KA
Department of Microbiology, and Immunology, School of Medicine and Biomedical Sciences, State University of New York, 138 Farber Hall, Buffalo, NY 14214, USA.
Yin
Lei
L
Thakur
Arvind
A
Christen
Linda
L
Niles
Edward G
EG
LoVerde
Philip T
PT
D000818:Animals; D006224:Cricetinae; D003600:Cytosol / Q000201:enzymology; D018076:DNA, Complementary / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000276:immunology*; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics; D016052:Protozoan Vaccines / Q000235:genetics* / Q000276:immunology*; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000235:genetics* / Q000276:immunology* / Q000469:parasitology; D015398:Signal Transduction / Q000235:genetics* / Q000276:immunology*; D013482:Superoxide Dismutase / Q000235:genetics* / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology
D018076:DNA, Complementary; D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase