Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies.
This review describes microfluidic systems in poly(dimethylsiloxane) (PDMS) for biological studies. Properties of PDMS that make it a suitable platform for miniaturized biological studies, techniques for fabricating PDMS microstructures, and methods for controlling fluid flow in microchannels are discussed. Biological procedures that have been miniaturized into PDMS-based microdevices include immunoassays, separation of proteins and DNA, sorting and manipulation of cells, studies of cells in microchannels exposed to laminar flows of fluids, and large-scale, combinatorial screening. The review emphasizes the advantages of miniaturization for biological analysis, such as efficiency of the device and special insights into cell biology.
Sia
Samuel K
SK
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Whitesides
George M
GM
D001695:Biology / Q000379:methods; D002477:Cells / Q000166:cytology; D004129:Dimethylpolysiloxanes; D008280:Magnetics; D044085:Microfluidics / Q000379:methods*; D012828:Silicones
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon