title: Effects of macromolecule synthesis inhibitors on light-induced phase shift of the circadian rhythm in melatonin release from the cultured pineal organ of a teleost, ayu (Plecoglossus altivelis).
abstract: Effects of macromolecule synthesis inhibitors on the light-induced phase shift of the circadian clock in the photoreceptive pineal organ of a teleost, ayu (Plecoglosus altivelis) were investigated using melatonin release as an indicator. A single light pulse during the early- and late-subjective night delayed and advanced the phase of the circadian rhythm in melatonin release, respectively. During the late subjective-night, protein synthesis inhibitor cycloheximide (CHX) delayed the rhythm while RNA synthesis inhibitor 5,6-dichlorobenzimidazole riboside (DRB) had little effect. Light-induced phase advance was diminished by the treatment of CHX but not by DRB. During the early subjective-night, DRB, CHX, light and combination of these (DRB+light, CHX+light) all phase-delayed the rhythm. There were no additive effects of light and DRB or CHX. These results indicate that macromolecule synthesis is somehow involved in generation of circadian oscillation, and that de novo protein synthesis is required for light-induced phase shift of the circadian clock in the ayu pineal organ.
authors: Laboratory of Aquatic Animal Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, jAPAN.
authors: Tomohiro
authors: T
authors: Masuda
authors: Masayuki
authors: M
authors: Iigo
authors: Kanta
authors: K
authors: Mizusawa
authors: Katsumi
authors: K
authors: Aida
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D002940:Circadian Rhythm / Q000187:drug effects* / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D008027:Light; D008550:Melatonin / Q000378:metabolism*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D036441:Osmeriformes / Q000502:physiology*; D010870:Pineal Gland / Q000378:metabolism*; D011863:Radioimmunoassay
chemical_list: C110962:5,6-dichlorobenzimidazole; D001562:Benzimidazoles; D019384:Nucleic Acid Synthesis Inhibitors; D003513:Cycloheximide; D008550:Melatonin