Preparation and characterization of macroporous chitosan-gelatin/beta-tricalcium phosphate composite scaffolds for bone tissue engineering.
A biodegradable composite scaffold was developed using beta-tricalcium phosphate (beta-TCP) with chitosan (CS) and gelatin (Gel) in the form of a hybrid polymer network (HPN) via co-crosslinking with glutaraldehyde. Various types of scaffolds were prepared by freezing and lyophilizing. These scaffolds were characterized by Fourier transform infrared, X-ray diffractometer, and scanning electron microscopy. The macroporous composite scaffolds exhibited different pore structures. Compressive properties were improved, especially compressive modulus from 3.9-10.9 MPa. Biocompatibility was evaluated subcutaneously on rabbits. A mild inflammatory response was observed over 12 weeks. The results suggest that the scaffolds can be utilized in nonloading bone regeneration.
Yin
Yuji
Y
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China.
Ye
Fen
F
Cui
Junfeng
J
Zhang
Fujiang
F
Li
Xiulan
X
Yao
Kangde
K
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D001673:Biodegradation, Environmental; D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D002130:Calcium Phosphates; D002465:Cell Movement / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003188:Composite Resins / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D005780:Gelatin; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000187:drug effects; D016062:Porosity; D019919:Prosthesis Implantation; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D003188:Composite Resins; C485817:beta-tricalcium phosphate; D002686:Chitin; D005780:Gelatin; D048271:Chitosan; C018392:tricalcium phosphate