A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.
Giot
L
L
CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA.
Bader
J S
JS
Brouwer
C
C
Chaudhuri
A
A
Kuang
B
B
Li
Y
Y
Hao
Y L
YL
Ooi
C E
CE
Godwin
B
B
Vitols
E
E
Vijayadamodar
G
G
Pochart
P
P
Machineni
H
H
Welsh
M
M
Kong
Y
Y
Zerhusen
B
B
Malcolm
R
R
Varrone
Z
Z
Collis
A
A
Minto
M
M
Burgess
S
S
McDaniel
L
L
Stimpson
E
E
Spriggs
F
F
Williams
J
J
Neurath
K
K
Ioime
N
N
Agee
M
M
Voss
E
E
Furtak
K
K
Renzulli
R
R
Aanensen
N
N
Carrolla
S
S
Bickelhaupt
E
E
Lazovatsky
Y
Y
DaSilva
A
A
Zhong
J
J
Stanyon
C A
CA
Finley
R L
RL
White
K P
KP
Braverman
M
M
Jarvie
T
T
Gold
S
S
Leach
M
M
Knight
J
J
Shimkets
R A
RA
McKenna
M P
MP
Chant
J
J
Rothberg
J M
JM
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism; D017344:Genes, Insect; D007113:Immunity, Innate; D008433:Mathematics; D015233:Models, Statistical; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020543:Proteome; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D029721:Drosophila Proteins; D020543:Proteome; D012333:RNA, Messenger; D066246:ErbB Receptors; D002118:Calcium