title: The effects of sodium phosphate and polyethylene glycol-electrolyte bowel preparation solutions on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat.
abstract: The aim of the present study is to evaluate the effects of sodium phosphate (NaP) and polyethylene glycol-electrolyte (PEG-EL) colon cleansing solutions on histopathology of 2,4,6-trinitrobenzene sulfonic acid (TNBS) and ethanol (E)-induced colitis in the rat and normal rat colon. The presence of hyperemia, inflammation and ulcer was evaluated to score of macroscopic morphologic damage. The microscopic criteria including ulceration, mucus cell depletion, crypt abscesses, inflammatory cysts, mucosal atrophy, edema, inflammatory cell infiltration, and vascular dilatation were evaluated to determine the extent of inflammatory reaction. Colitis findings were determined on the rats which were administered TNBS-E. Aphthoid lesions were seen 30% and 20 % of rats, respectively, by NaP and PEG-EL solutions in healthy group. Microscopic examination of aphthoid lesions revealed edema within the lamina propria and lymphoid hyperplasia in the mucosa and submucosa without erosion, ulceration and inflammatory cell infiltration. No significant difference was statistically found either macroscopically or microscopically in terms of the effects of saline, NaP and PEG-EL solutions in healthy rats and rats with colitis (p > .05). Under the present conditions, we concluded administration of NaP and PEG-EL solutions did not cause evident morphological changes on the rectal mucosa macroscopically and microscopically, although in a proportion of rats aphthoid lesions seem as a marker of mucosal damage macroscopically.
authors: Department of Pathology, Osmangazi University Faculty of Medicine, Eskisehir, Turkey.
authors: Bahattin
authors: B
authors: Erdogan
authors: Serap
authors: S
authors: Isiksoy
authors: Emine
authors: E
authors: Dundar
authors: Ozgul
authors: O
authors: Pasaoglu
authors: Cengiz
authors: C
authors: Bal
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000473:pathology*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004573:Electrolytes; D000431:Ethanol / Q000633:toxicity; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D010710:Phosphates / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions; D007507:Therapeutic Irrigation / Q000379:methods; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity; D014456:Ulcer / Q000139:chemically induced / Q000473:pathology
chemical_list: D004573:Electrolytes; D010710:Phosphates; D012996:Solutions; D000431:Ethanol; D011092:Polyethylene Glycols; D014302:Trinitrobenzenesulfonic Acid; C018279:sodium phosphate