Interaction of taxilin with syntaxin which does not form the SNARE complex.
Taxilin is novel binding partner of the syntaxin family, which is implicated in intracellular vesicle traffic. However, precise binding properties of taxilin to the syntaxin family remain to be clarified. Then, we here further investigated the interaction of taxilin with the syntaxin family by use of recombinant taxilins. Syntaxin-1a, -3, and -4 bound to taxilin in a dose-dependent and saturable manners. The concentrations of syntaxin-1a, -3, and -4 giving a half-maximal binding to taxilin were about 1.5, 3.0, and 1.0microM, respectively. The interaction of taxilin with syntaxin-1a was inhibited by SNAP-25 or Munc18 in a dose-dependent manner. When recombinant taxilin was incubated with the extract from the rat brain crude membrane fraction, recombinant taxilin bound to syntaxin-1s free of at least VAMP2, SNAP-25, and Munc18. These results suggest that taxilin interacts with the syntaxin family which does not form at least the SNARE complex.
Nogami
Satoru
S
Division of Molecular and Cell Biology, Institute for Medical Science, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu-machi, 321-0293, Tochigi, Japan.
Satoh
Sachie
S
Nakano
Michiko
M
Terano
Akira
A
Shirataki
Hiromichi
H
D000818:Animals; D000954:Antigens, Surface; D008565:Membrane Proteins / Q000737:chemistry*; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D011485:Protein Binding; D021381:Protein Transport; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins / Q000737:chemistry*
D000954:Antigens, Surface; D008565:Membrane Proteins; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D011994:Recombinant Proteins; D050600:SNARE Proteins; C494210:Snap25 protein, rat; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins; C476840:taxilin protein, rat