FT-Raman spectroscopic investigation of lens proteins of tilapia treated with dietary vitamin E.
FT-Raman spectroscopy was employed to explore the structural changes of lens proteins in Tilapia lenses affected by dietary vitamin E supplementation. The microenvironment of major lens constituents including thiol compounds, tyrosine, and tryptophan exhibited significant change upon vitamin E treatment, while the protein secondary structure was unaltered and remained as an antiparallel beta-pleated sheet. These structures in the cortex were more susceptible to vitamin E treatment than in the nucleus. Protein sulfhydryls in the cortex were predominantly in the reduced form, while in the nucleus both the oxidized and reduced forms coexisted as evidenced by the vibrational mode of SH (2580 cm(-1)) and SS (507 cm(-1)), respectively. Both tyrosine and tryptophan were more accessible to water or more exposed in the cortex than in the nucleus. The symmetrically inverse response of vitamin E, between Raman intensity of 1090 cm(-1) and the glutathione level, was consistent with a close relationship of GSH and vitamin E in defending the lens from external insults.
Shih
Shoeher
S
Department of Food Science, National Chiayi University, Chiayi, Taiwan.
Weng
Yih-Ming
YM
Chen
Shoeling
S
Huang
Sue-Lan
SL
Huang
Chen-Huei
CH
Chen
Wenlung
W
D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D003459:Crystallins / Q000378:metabolism*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D017210:Tilapia / Q000378:metabolism*; D014018:Tissue Distribution; D014364:Tryptophan / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage*
D003459:Crystallins; D014810:Vitamin E; D014364:Tryptophan; D005978:Glutathione