Canscora decussata (Roxb.) Schult (Gentianaceae) inhibits LPS-induced expression of ICAM-1 and E-selectin on endothelial cells and carageenan-induced paw-edema in rats.
Inflammation is characterized pathologically by an increased supply of blood to the affected area, vasodilation, and infiltration of phagocytic, monocytic, and polymorphonuclear cells into the site of inflammation. The infiltration of leukocytes is in part regulated by the increased expression of cell adhesion molecules viz. ICAM-1 and E-selectin on endothelial cells. We investigated the effect of ethanolic extract prepared from plant Canscora decussata on expression of ICAM-1 and E-selectin on endothelial cells. Our results demonstrated that treatment of human endothelial cells with the extract down-regulated the LPS-induced expression of ICAM-1 and E-selectin. To study further the anti-inflammatory activity of the extract, we evaluated its effect in a rat model of carageenan-induced paw-edema. Our results showed that the extract was able to reduce the edema formation in a dose-dependent manner. These results, therefore, indicate that Canscora decussata extract could be useful towards developing effective anti-inflammatory agent(s) in the future.
Madan
Babita
B
Molecular Immunology and Immunogenetics Laboratory, Institute of Genomics and Integrative Biology, University of Delhi Campus (North), Mall Road, Delhi 110007, India.
Mandal
B C
BC
Kumar
Sarvesh
S
Ghosh
B
B
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000096:biosynthesis*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D029965:Gentianaceae / Q000737:chemistry*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D019040:E-Selectin; D008070:Lipopolysaccharides; D010936:Plant Extracts; D018799:Intercellular Adhesion Molecule-1; D002351:Carrageenan