Schwann cells transplantation promoted and the repair of brain stem injury in rats.
OBJECTIVE
To explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats.


METHODS
Schwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively.


RESULTS
BrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas.


CONCLUSIONS
The transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.
Wan
Hong
H
Beijing Neurosurgical Institute, Tian Tan Xili 6, Beijing 100050, China. wanhong50@hotmail.com
An
Yi-Hua
YH
Sun
Mei-Zhen
MZ
Zhang
Ya-Zhuo
YZ
Wang
Zhong-Cheng
ZC
D000818:Animals; D000963:Antimetabolites; D001930:Brain Injuries / Q000628:therapy* / Q000662:veterinary; D001933:Brain Stem / Q000293:injuries*; D001973:Bromodeoxyuridine; D017690:Cell Transplantation / Q000379:methods* / Q000662:veterinary; D004594:Electrophysiology; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000637:transplantation*
D000963:Antimetabolites; D001973:Bromodeoxyuridine