title: Protective effects of scutellarin on superoxide-induced oxidative stress in rat cortical synaptosomes.
abstract: OBJECTIVE
  To evaluate the effects of scutellarin on superoxide-induced oxidative stress in rat cortical synaptosomes.
  
  
  METHODS
  Oxidative damage model was established by incubation with xanthine (0.3 mmol/L) and xanthine oxidase (0.02 U) at 37 degree for 30 min. The extent of membrane oxidation was assessed by malondialdehyde (MDA). Membrane fluidity was measured by fluorescence anisotropy (polarization) of the lipophilic probe 1,6-diphenyl-1,3,5-hexatriene (DPH). Intracellular Ca2+ concentration([Ca2+]i) was measured by fluorescent spectrophotometry. Fura 2-AM was used as an indicator for [Ca2+]i. Na+/K+-ATPase activity assay was based on the amount of inorganic phosphate (Pi) released during an enzymatic hydrolysis of ATP.
  
  
  RESULTS
  Synaptosomes exposed to superoxide significantly elevated the levels of malondialdehyde (MDA) and [Ca2+]i compared with those in normal group. These changes were accompanied by the decrease in membrane fluidity and Na+/K+-ATPase activity. Pretreatment with scutellarin (25-100 micromol/L) significantly ameliorated the oxidative damage of synaptosomes by reducing MDA levels and [Ca2+]i, up-regulating membrane fluidity and restoring Na+/K+-ATPase activity.
  
  
  CONCLUSIONS
  Scutellarin exerts a potent protective effect against oxidative damage in synaptosomes induced by superoxide.
authors: Pharmaceutical Institute, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Hong
authors: H
authors: Liu
authors: Xiang-Liang
authors: XL
authors: Yang
authors: Ying
authors: Y
authors: Wang
authors: Xiao-Qiao
authors: XQ
authors: Tang
authors: Dong-Ying
authors: DY
authors: Jiang
authors: Hui-Bi
authors: HB
authors: Xu
mesh_terms: D000818:Animals; D047310:Apigenin; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D005419:Flavonoids; D020011:Protective Agents; D008315:Malondialdehyde; D047310:Apigenin; D000254:Sodium-Potassium-Exchanging ATPase; C458179:scutellarein; D002118:Calcium