Conserved SNH domain of the proto-oncoprotein SYT interacts with components of the human chromatin remodelling complexes, while the QPGY repeat domain forms homo-oligomers.
Many studies have now established that the SWI/SNF chromatin remodelling complexes are involved in activation and repression of a variety of genes. In mammalian cells, these complexes contain the BRM and BRG1 helicase-like proteins that are thought to be responsible for nucleosome remodelling. The proto-oncoprotein SYT, involved in the unique translocation t(X;18) found in synovial sarcoma, is known to interact with human BRM (hBRM), thus providing a link between chromatin remodelling factors and human cancer. In this work, we address how SYT interacts with hBRM and BRG1. We demonstrate that the conserved N-terminal SNH domain of SYT, which is also present in the oncoproteins SYT-SSX, binds to both hBRM and BRG1. We have also found that in vivo the C-terminus transactivation QPGY region of SYT can interact with itself. This results in an amplified interaction with hBRM and highlights a possible regulatory function of this domain in cells.
Perani
Michela
M
Section of Molecular Carcinogenesis-BLB, Institute of Cancer Research, 15, Cotswold Road, Sutton, Surrey SM 5NG, UK. michela.perani@icr.ac.uk
Ingram
Catherine J E
CJ
Cooper
Colin S
CS
Garrett
Michelle D
MD
Goodwin
Graham H
GH
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D006801:Humans; D009496:Neurotensin / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D014157:Transcription Factors / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D002843:Chromatin; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; C084011:SMARCA2 protein, human; C093961:SS18 protein, human; D014157:Transcription Factors; C439005:stearyl-Nle(17)-neurotensin(6-11)VIP(7-28); D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin