title: Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients.
abstract: Pathogenetic processes that facilitate the entry, replication, and persistence of Mycobacterium tuberculosis (MTB) in the mammalian host likely include the regulated expression of specific sets of genes at different stages of infection. Identification of genes that are differentially expressed in vivo would provide insights into host-pathogen interactions in tuberculosis (TB); this approach might be particularly valuable for the study of human TB, where experimental opportunities are limited. In this study, the levels of selected MTB mRNAs were quantified in vitro in axenic culture, in vivo in the lungs of mice, and in lung specimens obtained from TB patients with active disease. We report the differential expression of MTB mRNAs associated with iron limitation, alternative carbon metabolism, and cellular hypoxia, conditions that are thought to exist within the granulomatous lesions of TB, in the lungs of wild-type C57BL/6 mice as compared with bacteria grown in vitro. Analysis of the same set of mRNAs in lung specimens obtained from TB patients revealed differences in MTB gene expression in humans as compared with mice.
authors: The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
authors: Juliano
authors: J
authors: Timm
authors: Frank A
authors: FA
authors: Post
authors: Linda-Gail
authors: LG
authors: Bekker
authors: Gabriele B
authors: GB
authors: Walther
authors: Helen C
authors: HC
authors: Wainwright
authors: Riccardo
authors: R
authors: Manganelli
authors: Wai-Tsing
authors: WT
authors: Chan
authors: Liana
authors: L
authors: Tsenova
authors: Benjamin
authors: B
authors: Gold
authors: Issar
authors: I
authors: Smith
authors: Gilla
authors: G
authors: Kaplan
authors: John D
authors: JD
authors: McKinney
mesh_terms: D000818:Animals; D002244:Carbon / Q000378:metabolism; D015687:Cell Hypoxia; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005798:Genes, Bacterial; D005943:Gluconeogenesis; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D005227:Fatty Acids; D012329:RNA, Bacterial; D012333:RNA, Messenger; D002244:Carbon; D007501:Iron; D010100:Oxygen