Remarkable cross-reaction of pan-pestivirus PCR primers with poliovirus genome.
When several human vaccines were tested for pestivirus contamination using a one-tube closed nested RT-PCR method employing pan-pestivirus primers selected from the 5'-untranslated region (5'-UTR) of the pestivirus genome, a 224 bp DNA product was produced from a poliovirus vaccine. Although this amplicon was of the size expected for pestiviruses, its sequence showed a 100% similarity with the corresponding reverse complement of a nucleotide sequence from the VP2 gene of the poliovirus type 1 Sabin strain. It is recommended that all positive PCR products, especially those prepared using pan-pestivirus primers, obtained from screening biological substances for pestivirus contamination should be checked by use of a specific hybridization probe and preferably by sequencing.
Vilcek
Stefan
S
Department of Parasitology and Infectious Diseases, University of Veterinary Medicine, Komenskeho 73, SK-041 81 Kosice, Slovak Republic. vilcek@uvm.sk
Nettleton
Peter
P
Paton
David
D
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers; D004340:Drug Contamination; D006801:Humans; D010576:Pestivirus / Q000235:genetics*; D017955:Poliovirus / Q000235:genetics*; D011055:Poliovirus Vaccine, Oral; D023321:Poliovirus Vaccines; D016133:Polymerase Chain Reaction / Q000379:methods*
D036022:Capsid Proteins; D017931:DNA Primers; D011055:Poliovirus Vaccine, Oral; D023321:Poliovirus Vaccines