title: Progenitor cell involvement is predictive of response to induction chemotherapy in paediatric acute myeloid leukaemia.
abstract: In acute myeloid leukaemia (AML), involvement of early progenitor cells may predict poor response to induction chemotherapy. We evaluated the involvement of early progenitor cells in two AML subtypes with a favourable prognosis [t(8;21) and t(15;17)], and a subtype with poor prognosis (monosomy 7). CD34+CD33- cells were isolated by fluorescence-activated cell sorting, grown in liquid medium followed by culture in semi-solid medium, and the colonies that were formed were analysed for the identifiable genetic markers. Two of 136 colonies from six t(8;21) AML patients expressed the AML1-ETO transcript, and all six patients achieved remission after induction. None of 192 colonies from five t(15;17) AML patients expressed the RARalpha-PML transcript and all achieved remission. In contrast, in three of 10 cases of monosomy 7 AML, colonies were positive for monosomy 7, and all three patients failed to enter remission. However, five of six evaluable patients with colonies negative for monosomy 7 entered remission. These data support the hypothesis that leukaemic involvement of early progenitor cells affects the response to induction chemotherapy.
authors: Fred Hutchinson Cancer Research Center, Seattle, WA, USA. djohnston@cheo.on.ca
authors: Donna L
authors: DL
authors: Johnston
authors: Soheil
authors: S
authors: Meshinchi
authors: Kent E
authors: KE
authors: Opheim
authors: Maria G
authors: MG
authors: Pallavicini
authors: James
authors: J
authors: Feusner
authors: William G
authors: WG
authors: Woods
authors: Beverly J
authors: BJ
authors: Lange
authors: Jerald P
authors: JP
authors: Radich
authors: Irwin D
authors: ID
authors: Bernstein
mesh_terms: D000208:Acute Disease; D002648:Child; D002869:Chromosome Aberrations; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002891:Chromosomes, Human, Pair 21; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D050676:Core Binding Factor Alpha 2 Subunit; D015321:Gene Rearrangement; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000188:drug therapy* / Q000235:genetics*; D009006:Monosomy; D009363:Neoplasm Proteins / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D011379:Prognosis; D000075142:RUNX1 Translocation Partner 1 Protein; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D005819:Genetic Markers; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D000075142:RUNX1 Translocation Partner 1 Protein; D014157:Transcription Factors; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein