title: Fibrinogen conformation and platelet reactivity in relation to material-blood interaction: effect of stress hormones.
abstract: The performance of many biomaterials in hemocompatibility tests is altered when blood is drawn from stressed subjects. A salient physiological response during stress is one in which hormones are released into plasma by the hypothalamo-pituitary-adrenal axis. We investigated the influence of basal and stress levels of epinephrine and beta-endorphin on the conformation of fibrinogen (Fbg), both in saline solution (under physiological conditions) and after its adsorption to polyethylene (PE), by FT-IR spectroscopy. Moreover, as Fbg is one of the major mediators of platelet adhesion, the behavior of platelets in contact with PE was also evaluated as a function of the two different hormone concentrations. Epinephrine was found to affect Fbg conformation and to increase platelet adhesion to PE at stress level. Basal and stress levels of beta-endorphin did not significantly affect the Fbg conformation and only induced adhesion of isolated platelets to the PE surface. A direct relationship was therefore found between Fbg conformation and platelet behavior. The response of platelets was affected by the stress status of donors through the influence of epinephrine on Fbg conformation.
authors: C.R.I.S.M.A. and Department of Chemical and Biosystem Sciences and Technologies, University of Siena, Via Aldo Moro 2, Siena, Italy 53100. barbucci@unisi.it
authors: Rolando
authors: R
authors: Barbucci
authors: Stefania
authors: S
authors: Lamponi
authors: Agnese
authors: A
authors: Magnani
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000494:pharmacology / Q000592:standards*; D001782:Blood Donors / Q000523:psychology; D004837:Epinephrine / Q000097:blood; D005340:Fibrinogen / Q000737:chemistry*; D006728:Hormones / Q000097:blood; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects*; D020959:Polyethylene / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological / Q000097:blood*; D001615:beta-Endorphin / Q000097:blood
chemical_list: D001672:Biocompatible Materials; D006728:Hormones; D001615:beta-Endorphin; D005340:Fibrinogen; D020959:Polyethylene; D004837:Epinephrine