New directions of miniaturization within the proteomics research area.
An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel- and liquid-phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing plays a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.
Marko-Varga
György
G
Department of Analytical Chemistry, Lund University, Lund, Sweden. gyorgy.marko-varga@astrazeneca.com
Nilsson
Johan
J
Laurell
Thomas
T
D008904:Miniaturization / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods* / Q000639:trends; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome