title: Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea.
abstract: Biomass samples from the Black Sea collected in 1988 were analyzed for SSU genes from Bacteria and Archaea after 10 years of storage at -80 degrees C. Both clonal libraries and direct fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analyses were used to assess the microbial community. Uniform and discrete depth distributions of different SSU phylotypes were observed. However, most recombinant clones were not restricted to a specific depth in the water column, and many of the major T-RFLP peaks remain uncharacterized. Of the clones obtained, an epsilon-Proteobacteria and a Pseudoalteromonas-like clone accounted for major peaks in the fingerprint, while deeply branching lineages of alpha- and gamma-Proteobacteria were associated with smaller peaks. Additionally, members were found among both the delta-Proteobacteria related to sulfate reducers and the Archaea related to phylotypes from the ANME groups that anaerobically oxidize methane.
authors: Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey 08901-8525, USA.
authors: Costantino
authors: C
authors: Vetriani
authors: Hiep V
authors: HV
authors: Tran
authors: Lee J
authors: LJ
authors: Kerkhof
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001105:Archaea / Q000145:classification* / Q000235:genetics; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D018533:Biomass; D016172:DNA Fingerprinting / Q000379:methods*; D004275:DNA, Ribosomal; D017753:Ecosystem; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D020560:Proteobacteria / Q000145:classification / Q000235:genetics; D031161:Pseudoalteromonas / Q000145:classification / Q000235:genetics; D012336:RNA, Ribosomal, 16S; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S