A Bacillus thuringiensis isolate possessing a spore-associated filament.
We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast and electron microscopy indicate the presence of both a parasporal body and a long filament which are retained after sporulation. The filament is shown to consistently arise from the end of the exosporium and next to the parasporal body. Upon spore germination, the parasporal body/filament complex is retained on the cell wall of the resulting bacterium.
Rampersad
Joanne
J
The University of the West Indies, School of Agriculture, Trinidad, West Indies.
Khan
Ayub
A
Ammons
David
D
D001413:Bacillus thuringiensis / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000648:ultrastructure*; D001427:Bacterial Toxins / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure*; D014300:Trinidad and Tobago
D001427:Bacterial Toxins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S