title: cDNA cloning and expression analysis of the rice (Oryza sativa L.) RNase L inhibitor.
abstract: A subtractive cDNA library was constructed using rice (Oryza sativa L.) callus cDNA as driver and differentiating callus cDNA as tester. A novel cDNA fragment encoding RNase L inhibitor (RLI) was isolated by screening the subtractive library, which had a higher expression level in differentiating callus than in callus. The full-length cDNA of rice-RLI was obtained by the method of rapid amplification of cDNA ends, which contained a 1812-bp open reading frame encoding a 604 amino acid polypeptide. Homologous analysis showed that rice-RLI contained the conserved motifs (two repeated P-loops, two ATP-binding boxes and an iron-sulfur binding motif). The fluorescence quantitative PCR analysis showed that it was a constitutive expressed gene but up-regulated in abiotic stress (low temperature and NaCl treatment) and down-regulated under the treatments of NAA and IAA.
authors: Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors: Xi-Ling
authors: XL
authors: Du
authors: Dong
authors: D
authors: Wang
authors: Xiao-Yin
authors: XY
authors: Qian
authors: Li-Zhi
authors: LZ
authors: Jiang
authors: Wei
authors: W
authors: Chun
authors: Ke-Gui
authors: KG
authors: Li
authors: Guo-An
authors: GA
authors: Shen
authors: Chang-Fa
authors: CF
authors: Lin
authors: Jin-Shui
authors: JS
authors: Yang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D018833:Chaperonins / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005453:Fluorescence; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature; D013997:Time Factors
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D018076:DNA, Complementary; D012965:Sodium Chloride; D018833:Chaperonins