DNase I treatment of cDNA first strands prevents RT-PCR amplification of contaminating DNA sequences.
Flohr
Aljoscha M
AM
Center for Human Genetics, University of Bremen, Bremen, Germany.
Hackenbeck
Thomas
T
Schlueter
Claudia
C
Rogalla
Piere
P
Bullerdiek
Joern
J
D000595:Amino Acid Sequence; D016477:Artifacts; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism*; D004340:Drug Contamination / Q000517:prevention & control*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011786:Quality Control; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D003850:Deoxyribonuclease I