A fluorescent carbazole derivative: high sensitivity for quadruplex DNA.
We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T(2)AG(3))(4). The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 microM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 microM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti-anti-anti-anti and anti-anti-syn-syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti-syn-anti-syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T(2)AG(3))(4) can be revealed by BMVC.
Chang
Cheng-Chung
CC
Institute of Atomic and Molecular Sciences, Academia Sinica, PO Box 23-166, Taipei, 10764, Taiwan, Republic of China.
Wu
Jin-Yi
JY
Chien
Chih-Wei
CW
Wu
Wei-Sung
WS
Liu
Heng
H
Kang
Chi-Chih
CC
Yu
Liang-Jye
LJ
Chang
Ta-Chau
TC
D000042:Absorption; D002227:Carbazoles / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D054856:G-Quadruplexes; D006801:Humans; D011726:Pyridinium Compounds / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D016615:Telomere / Q000235:genetics; D017186:Titrimetry
C486012:3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide; D002227:Carbazoles; D011726:Pyridinium Compounds; C041514:carbazole; D004247:DNA