Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana.
Cytokinins have been implicated in developmental and growth processes in plants including cell division, chloroplast biogenesis, shoot meristem initiation and senescence. The regulation of these processes requires changes in cytokinin-responsive gene expression. Here, we induced the expression of a bacterial isopentenyl transferase gene, IPT, in transgenic Arabidopsis thaliana seedlings to study the regulation of genome-wide gene expression in response to endogenous cytokinin. Using MPSS (massively parallel signature sequencing) we identified 823 and 917 genes that were up- and downregulated, respectively, following 24 h of IPT induction. When comparing the response to cytokinin after 6 and 24 h, we identified different clusters of genes showing a similar course of regulation. Our study provides researchers with the opportunity to rapidly assess whether genes of interest are regulated by cytokinins.
Hoth
Stefan
S
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. shoth@biologie.uni-erlangen.de
Ikeda
Yoshihisa
Y
Morgante
Michele
M
Wang
Xiujie
X
Zuo
Jianru
J
Hanafey
Michael K
MK
Gaasterland
Terry
T
Tingey
Scott V
SV
Chua
Nam-Hai
NH
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005810:Multigene Family / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000302:isolation & purification; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D003583:Cytokinins; D018749:RNA, Plant; D014157:Transcription Factors; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase