Study on metabolism of scutellarin in rats by HPLC-MS and HPLC-NMR.
Scutellarin is the major active constituent of Scutellaria barbata D. The metabolism of scutellarin has been investigated in rats. The solid-phase extraction and HPLC-DAD methods were established to separate and analyse metabolites. Five metabolites (M1-M5) were identified by enzymatic hydrolysis, HPLC-DAD, HPLC-MS and HPLC-NMR. M1 and M3 were conjugates of scutellarin with two sulfate groups, which have not been reported in natural plants. M2 was scutellarin; M4 was 6-methyl-scutellarin; and M5 was 6-methyl-scutellarein. The metabolic pathway was proposed.
Zhang
Jin-Lan
JL
Institute of Materia Medica, Chinese Academy of Medical Sciences 100050, China. zhjl2002by@sohu.com
Che
Qing-Min
QM
Li
Shou-Zhuo
SZ
Zhou
Tong-Hui
TH
D000284:Administration, Oral; D000818:Animals; D047310:Apigenin; D001646:Bile / Q000378:metabolism; D005419:Flavonoids / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use / Q000652:urine; D005965:Glucuronates; D008099:Liver / Q000378:metabolism*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D031363:Scutellaria
D005419:Flavonoids; D005965:Glucuronates; D010936:Plant Extracts; C484876:scutellarin; D047310:Apigenin; C458179:scutellarein