Mycophenolate mofetil inhibits human Tenon fibroblast proliferation by guanosine depletion.
OBJECTIVE
Mycophenolate mofetil could be a useful antiproliferative drug in glaucoma filtering surgery. In this study the authors set out to investigate whether growth inhibition of mycophenolate mofetil on human Tenon fibroblasts is mediated by guanosine depletion.


METHODS
Cultured human Tenon fibroblasts were incubated in various concentrations of mycophenolate mofetil with and without supplementation of guanosine.


RESULTS
Growth inhibition was concentration dependent. The effect could be completely antagonised by guanosine supplement.


CONCLUSIONS
Human Tenon fibroblasts depend on the de novo synthesis pathway of guanosine. No sufficient salvage pathway from purine degradation appears to exist.
Heinz
C
C
Department of Ophthalmology, University of Essen, Hufelandstrasse 55, 45122 Essen, Germany. carsten.heinz@gmx.net
Heise
K
K
Hudde
T
T
Steuhl
K-P
KP
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006151:Guanosine / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007168:IMP Dehydrogenase / Q000037:antagonists & inhibitors; D007166:Immunosuppressive Agents / Q000378:metabolism / Q000494:pharmacology*; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D014130:Trabeculectomy
D007166:Immunosuppressive Agents; D006151:Guanosine; D007168:IMP Dehydrogenase; D009173:Mycophenolic Acid