title: Numerical study of induced current perturbations in the vicinity of excitable cells exposed to extremely low frequency magnetic fields.
abstract: Realistic three-dimensional cell morphologies were modelled to determine the current density induced in excitable cell culture preparations exposed to 60 Hz magnetic fields and to identify important factors that can influence the responses of cells to these fields. Cell morphologies representing single spherical adrenal chromaffin cells, single elongated smooth muscle cells and chromaffin cell aggregates in a Petri dish containing culture medium were modelled using the finite element method. The computations for a spherical cell revealed alterations in the magnitude and spatial distribution of the induced current density in the immediate vicinity of the cell. Maxima occurred at the equatorial sides and minima at the poles. Proximity of cells to each other as well as cell aggregate shape, size and orientation with respect to the induced current influenced the magnitude and spatial distribution of the induced current density. For an elongated cell, effects on the induced current density were highly dependent on cell orientation with respect to the direction of the induced current. These results provide novel insights into the perturbations in induced current that occur in excitable cell culture preparations and lay a foundation for understanding the mechanisms of interaction with extremely low frequency magnetic fields at the tissue level.
authors: Biomedical Engineering Graduate Program, University of Nevada, Reno, NV 89557, USA.
authors: Noha
authors: N
authors: Hassan
authors: Indira
authors: I
authors: Chatterjee
authors: Nelson G
authors: NG
authors: Publicover
authors: Gale L
authors: GL
authors: Craviso
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000528:radiation effects; D002462:Cell Membrane / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D019439:Chromaffin Cells / Q000528:radiation effects*; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D008954:Models, Biological; D009131:Muscle, Smooth, Vascular / Q000528:radiation effects*; D010784:Photomicrography