title: Immunolocalization of laminin-alpha1-like antigens around synapses in mouse cerebellar perineuronal nets.
abstract: The hypothesis that extracellular matrix components may be related to neuronal development in the mouse cerebellar cortex was verified with immunohistochemistry by using an antibody against laminin-alpha1, a major extracellular matrix protein in various tissues. A commercially available polyclonal antibody, raised against the carboxyl-terminal 20-amino acid peptide of laminin-alpha1 was used. Some positive immunoreaction products were localized around large GABAergic interneurons in granular layers and others were around neurons in deep cerebellar nuclei. At the electron microscope level, diaminobenzidine immunoreaction products were localized around presynaptic boutons and in intercellular matrices around interneurons. Such immunoreaction products could be detected at postnatal day 20, when most of cerebellar synapses are assumed to be established. It has been known that a special feature of extracellular matrix, termed perineuronal nets, exists around specific subpopulation of neurons. In the mouse cerebellum, the present findings suggest that laminin itself or laminin-like-antigens exists in the perineuronal nets in relation to inhibitory neuron synapses.
authors: Department of Anatomy, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi 409-3898, Japan.
authors: Takashi
authors: T
authors: Yagi
authors: Nobuo
authors: N
authors: Terada
authors: Takeshi
authors: T
authors: Baba
authors: Shinichi
authors: S
authors: Ohno
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D007395:Interneurons / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D007797:Laminin / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D017729:Presynaptic Terminals / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D007797:Laminin; C084098:laminin A