title: Layering defect in p35 deficiency is linked to improper neuronal-glial interaction in radial migration.
abstract: Several genes essential for neocortical layering have been identified in recent years, but their precise roles in this process remain to be elucidated. Mice deficient in p35--an activator of cyclin-dependent kinase 5 (Cdk5)--are characterized by a neocortex that has inverted layering. To decipher the physiological mechanisms that underlie this defect, we compared time-lapse recordings between p35(-/-) and wild-type cortical slices. In the p35(-/-) neocortex, the classic modes of radial migration--somal translocation and locomotion--were largely replaced by a distinct mode of migration: branched migration. Branched migration is cell-autonomous, associated with impaired neuronal-glial interaction and rare in neurons of scrambler mice, which are deficient in Dab1. Hence, our findings suggest that inside-out layering requires distinct functions of Reelin and p35/Cdk5 signaling, with the latter being important for proper glia-guided migration.
authors: Department of Pathology, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA.
authors: Amitabh
authors: A
authors: Gupta
authors: Kamon
authors: K
authors: Sanada
authors: David T
authors: DT
authors: Miyamoto
authors: Susan
authors: S
authors: Rovelstad
authors: Bagirathy
authors: B
authors: Nadarajah
authors: Alan L
authors: AL
authors: Pearlman
authors: Jan
authors: J
authors: Brunstrom
authors: Li-Huei
authors: LH
authors: Tsai
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D000091042:Reelin Protein
chemical_list: D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C089495:neuronal Cdk5 activator (p25-p35); C000717128:Reln protein, mouse