Metabolism of grape seed polyphenol in the rat.
The metabolism of grape seed polyphenol (GSP) has been investigated in rats by high-performance liquid chromatography analysis of the serum and urinary concentrations of the GSP metabolites (+)-catechin (CT), (-)-epicatechin (EC), 3'-O-methyl-(+)-catechin, and 3'-O-methyl-(-)-epicatechin. The serum concentration of these four metabolites reached a maximum 3 h after the oral administration of GSP. The urinary excretion of these GSP metabolites accounted for 0.254% (w/w) of the administered dose of GSP (1.0 g/kg), and the majority of these metabolites were excreted within 25 h of oral administration. The serum concentration and urinary excretion of these metabolites were also compared after the oral administration of different GSP monomers (gallic acid, CT, and EC), normal GSP, and the high molecular weight components of GSP (GSPH). No metabolites were detected in the serum of rats given GSPH. The urinary percentage excretion of the GSP metabolites derived from the respective monomers (CT or EC) did not vary with the administration of different substances (CT or EC, GSP, or GSPH). Taken together, these results suggest that only the monomers of GSP are absorbed and metabolized.
Nakamura
Yumiko
Y
National Institute of Health Sciences, Osaka Branch, 1-1-43, Hoenzaka, Chuo-ku, Osaka 540-0006, Japan. nakamura-y@city.takatuki.osaka.jp
Tonogai
Yasuhide
Y
D002392:Catechin / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D005419:Flavonoids / Q000493:pharmacokinetics*; D008970:Molecular Weight; D010636:Phenols / Q000493:pharmacokinetics*; D059808:Polyphenols; D012639:Seeds / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
C480347:3'-O-methylcatechin; C424450:3'-O-methylepicatechin; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D002392:Catechin