title: Identification of hard tissue after experimental pulp capping using dentin sialoprotein (DSP) as a marker.
abstract: The purpose of this study was to identify the hard tissue formed early in experimental pulp exposures capped with mineral trioxide aggregate (MTA) or bone morphogenetic protein (BMP)-7 using dentin sialoprotein (DSP) as a marker. The pulps of 35 maxillary first, second, and third molar teeth from 10 male rats were experimentally exposed. The pulps were capped with MTA alone as a pulp-capping agent and final restoration or with BMP-7 followed by restoration with MTA. Five teeth with class I occlusal preparations, no exposure, and no restoration served as positive controls. Five teeth that received pulp exposures and no restoration served as negative controls. Five untreated third molars served as additional controls. The animals were killed at 2 weeks. The specimens were prepared and evaluated histologically and with immunohistochemistry using polyclonal antibodies raised against rat DSP. Pulps capped with MTA formed hard tissue that demonstrated significantly more immunostaining for DSP compared with BMP-7 (p = 0.0031). MTA-capped pulps also showed significantly more complete bridge formation compared with BMP-7 (p = 0.0008). Pulps capped with BMP-7 demonstrated a hard tissue that was bone-like in appearance and devoid of DSP staining.
authors: Department of Endodontics, Loma Linda University School of Dentistry, CA 92350, USA.
authors: Wallis E
authors: WE
authors: Andelin
authors: Shahrokh
authors: S
authors: Shabahang
authors: Kenneth
authors: K
authors: Wright
authors: Mahmoud
authors: M
authors: Torabinejad
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000503:physiopathology* / Q000628:therapy; D003809:Dentin, Secondary / Q000187:drug effects / Q000378:metabolism*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins; D011498:Protein Precursors; D051381:Rats; D017207:Rats, Sprague-Dawley; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012795:Sialoglycoproteins; D017640:Silicates / Q000494:pharmacology*; D016212:Transforming Growth Factor beta; D000099311:Dentin Sialophosphoprotein
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D017610:Calcium Compounds; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D010087:Oxides; D010750:Phosphoproteins; D011498:Protein Precursors; D012387:Root Canal Filling Materials; D012795:Sialoglycoproteins; D017640:Silicates; D016212:Transforming Growth Factor beta; D000099311:Dentin Sialophosphoprotein; C525821:Bmp7 protein, rat; C086631:mineral trioxide aggregate