title: Oligomeric assembly and ligand binding of the members of protein family YER057c/YIL051c/YJGF.
abstract: Proteins UK114 and p14.5 are both members of the putative family of small proteins YER057c/YIL051c/YjgF. The biological role of these proteins is not understood very well, and in addition, their oligomeric structure in solution remains controversial. We therefore investigated the oligomeric structure of UK114 and p14.5 using a number of methods. Both proteins have exhibited a homotrimeric structure in solution. Indeed the trimeric structure of the two proteins appeared to be so similar that when protein subunits derived from different species were mixed, stable heterotrimeric complexes (monomer ratio of 1:2 and 2:1 of UK114 and p14.5, respectively) could be formed in vitro. Furthermore, the trimeric structure of both UK114 and p14.5 proved essential for the stoichiometric hydrophobic ligand, such as fatty acid binding activity of the two proteins.
authors: Institute of Biotechnology, V. Graiciūno 8, Vilnius 2028, Lithuania. Editami@ibt.lt
authors: Edita
authors: E
authors: Mistiniene
authors: Virginijus
authors: V
authors: Luksa
authors: Jolanta
authors: J
authors: Sereikaite
authors: Vytautas
authors: V
authors: Naktinis
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000817:Anilino Naphthalenesulfonates; D005227:Fatty Acids; D006360:Heat-Shock Proteins; D008024:Ligands; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; C101630:UK114 antigen; C103185:trichloroacetic acid soluble protein, 14.5 kDa; C027132:1-anilino-8-naphthalenesulfonate