The functions of Klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye.
Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTOC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm(0), which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTOC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies.
Patterson
Kristin
K
Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA.
Molofsky
Ari B
AB
Robinson
Christina
C
Acosta
Shelley
S
Cater
Courtney
C
Fischer
Janice A
JA
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004330:Drosophila / Q000166:cytology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005123:Eye / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D034882:Lamins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D022101:Microtubule-Organizing Center / Q000378:metabolism; D015530:Nuclear Matrix / Q000378:metabolism; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology* / Q000378:metabolism
D029721:Drosophila Proteins; D034882:Lamins; D026901:Membrane Transport Proteins; C401521:klar protein, Drosophila