title: Temperature-dependent stability and translation of Escherichia coli ompA mRNA.
abstract: RNase E is known to affect the turnover of ompA mRNA in a growth rate-dependent manner. Here, we show that this enzyme also plays a role in the temperature-dependent stability of the transcript, thereby maintaining comparable levels of OmpA at 28 and 37 degrees C. An increase in the efficiency of RNase E cleavages at 37 degrees C within the 5(') UTR of the transcript in vitro was found to correlate with a decreased half-life and steady-state level at elevated temperature in vivo. However, measurements of de novo OmpA synthesis and in vitro toeprinting experiments suggest that translation of ompA mRNA is more efficient at 37 degrees C when compared to 28 degrees C. Thus, the enhanced translation apparently counteracts the decreased half-life at elevated temperature. Moreover, we propose that the temperature-dependent inverse correlation between ompA mRNA stability and translation can result from structural changes induced in the 5(') UTR of the transcript.
authors: Department of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9/4, A-1030 Vienna, Austria.
authors: Taras
authors: T
authors: Afonyushkin
authors: Isabella
authors: I
authors: Moll
authors: Udo
authors: U
authors: Bläsi
authors: Vladimir R
authors: VR
authors: Kaberdin
mesh_terms: D020121:5' Untranslated Regions; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002455:Cell Division; D004722:Endoribonucleases / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D020121:5' Untranslated Regions; D001425:Bacterial Outer Membrane Proteins; D012333:RNA, Messenger; C078167:OMPA outer membrane proteins; D004722:Endoribonucleases; C022537:ribonuclease E