Different polymerase chain reaction-based analyses for culture-negative endocarditis caused by Streptococcus pneumoniae.
Two cases of culture-negative infective endocarditis caused by Streptococcus pneumoniae are presented. Conventional bacteriological methods were compared with 2 different polymerase chain reaction (PCR)-based analyses: PCR amplification with primers specific for S. pneumoniae, and broad-range PCR followed by sequencing. PCR-based analyses can be a valuable supplement to traditional bacteriological analyses.
Voldstedlund
Marianne
M
Department of Clinical Microbiology, Skejby Hospital, Brendstrupgaardsvej, Aarhus, Denmark. mvold@dadlnet.dk
Pedersen
Lisbeth Nørum
LN
Fuursted
Kurt
K
Nielsen
Lars P
LP
D000368:Aged; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial