title: The construction of an EST database for Bombyx mori and its application.
abstract: To build a foundation for the complete genome analysis of Bombyx mori, we have constructed an EST database. Because gene expression patterns deeply depend on tissues as well as developmental stages, we analyzed many cDNA libraries prepared from various tissues and different developmental stages to cover the entire set of Bombyx genes. So far, the Bombyx EST database contains 35,000 ESTs from 36 cDNA libraries, which are grouped into approximately 11,000 nonredundant ESTs with the average length of 1.25 kb. The comparison with FlyBase suggests that the present EST database, SilkBase, covers >55% of all genes of Bombyx. The fraction of library-specific ESTs in each cDNA library indicates that we have not yet reached saturation, showing the validity of our strategy for constructing an EST database to cover all genes. To tackle the coming saturation problem, we have checked two methods, subtraction and normalization, to increase coverage and decrease the number of housekeeping genes, resulting in a 5-11% increase of library-specific ESTs. The identification of a number of genes and comprehensive cloning of gene families have already emerged from the SilkBase search. Direct links of SilkBase with FlyBase and WormBase provide ready identification of candidate Lepidoptera-specific genes.
authors: Laboratory of Insect Genome, National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan.
authors: Kazuei
authors: K
authors: Mita
authors: Mitsuoki
authors: M
authors: Morimyo
authors: Kazuhiro
authors: K
authors: Okano
authors: Yoshiko
authors: Y
authors: Koike
authors: Junko
authors: J
authors: Nohata
authors: Hideki
authors: H
authors: Kawasaki
authors: Keiko
authors: K
authors: Kadono-Okuda
authors: Kimiko
authors: K
authors: Yamamoto
authors: Masataka G
authors: MG
authors: Suzuki
authors: Toru
authors: T
authors: Shimada
authors: Marian R
authors: MR
authors: Goldsmith
authors: Susumu
authors: S
authors: Maeda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins