Functional and expression analyses of mgl-1, a mouse orthologue of lethal giant larvae recessive oncogene.
We recently isolated a few mammalian homologue of Drosophila lethal giant larvae (lgl) recessive oncogene, suggesting that there is functional conservation among proteins of this family. The comparison of amino acid sequence for mgl-1 with other lgl family members using the clustal method showed that they are highly homologous. Therefore, we investigated the biological function of mgl-1, a mouse orthologue of lgl, in the absence of Saccharomyces cerevisiae Sop1 and Sop2, the yeast homologues of the lgl recessive oncogene. Functional analysis showed that the expression of mgl-1 cDNA partially restored salt tolerance in yeast, indicating the evolutionary conservation of lgl family members. Since the developmental expression profile of mgl-1 has not been elucidated, the temporal and spatial expression patterns of mouse mgl-1 during early embryonic development were analyzed. The temporal expression analysis revealed that mgl-1 is expressed throughout embryonic development from days E4.5 to E18.5 with the strong expression at E10.5. The analysis of spatial expression showed that mgl-1 mRNA is detected in CNS, craniofacial region, eyes, limbs, and the gut.
Kim
Yong-Soo
YS
Cell and Gene Therapy Research Institute, Pochon CHA University, CHA General Hospital, Seoul, Korea.
Song
Jihwan
J
Kim
Yukyung
Y
Kim
In-Ok
IO
Kang
Incheol
I
Baek
Kwang-Hyun
KH
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018507:Gene Expression Regulation, Developmental; D005816:Genetic Complementation Test; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000502:physiology; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D010744:Phosphoric Monoester Hydrolases; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000502:physiology
D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D018398:Homeodomain Proteins; C082939:Llgl1 protein, mouse; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts; D025521:Tumor Suppressor Proteins; C110553:INP53 protein, S cerevisiae; D010744:Phosphoric Monoester Hydrolases