Microencapsulation of superoxide dismutase into poly(epsilon-caprolactone) microparticles by reverse micelle solvent evaporation.
The aim of this work was to encapsulate superoxide dismutase (SOD) in poly(epsilon-caprolactone) (PCL) microparticles by reverse micelle solvent evaporation. The concentration of PCL, the hydrophile-lipophile balance (HLB), and concentration of the sucrose ester used as surfactant in the organic phase were investigated as formulation variables. Relatively higher encapsulation efficiency (approximately 48%) and retained enzymatic activity (>90%) were obtained with microparticle formulation made from the 20% (w/v) PCL and 0.05% (w/v) sucrose ester of HLB = 6. This formulation allowed the in vitro release of SOD for at least 72 hr. These results showed that reverse micelle solvent evaporation can be used to efficiently encapsulate SOD in PCL microparticles. Such formulations may improve the bioavailability of SOD.
Youan
Bi-Botti Célestin
BB
Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA. bcyouan@ama.ttuhsc.edu
D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D008823:Micelles; D011091:Polyesters / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D012997:Solvents / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D013482:Superoxide Dismutase / Q000138:chemical synthesis* / Q000493:pharmacokinetics
D002214:Capsules; D008823:Micelles; D011091:Polyesters; D012997:Solvents; C016240:polycaprolactone; D013482:Superoxide Dismutase