title: Porcine peripheral blood dendritic cells and natural interferon-producing cells.
abstract: Peripheral blood contains two major particular infrequent dendritic cells (DC) subsets linking the innate and specific immune system, the myeloid DC and plasmacytoid DC equivalent to the natural interferon-producing cells (NIPC). The functional characterization of these cells demands large volumes of blood, making a large animal model more appropriate and beneficial for certain studies. Here, two subsets of porcine blood mononuclear cells expressing swine workshop cluster 3 (SWC3, a SIRP family member), are described and compared to monocytes. The blood DC specialized in T-cell stimulation were major histocompatibility complex (MHC) class II+, CD80/86+, CD1+/-, CD4-, and in contrast to monocytes CD14-. A CD16- and a CD16+ subset could be discriminated. Granulocyte-macrophage colony-stimulating factor and interleukin-3 were survival factors for this DC subset, and culture induced an up-regulation of MHC class II and CD80/86. The second subset described, are porcine NIPC, typically CD4++, MHC class IIlow, CD80/86low, CD1-, CD8-/low, CD16-/low and CD45RA-/low. Porcine NIPC had high interleukin-3 binding capacity, and survived in response to this cytokine. Their unique function was strong interferon type I secretion after virus stimulation. Both subsets were endocytically active when freshly isolated, and down-regulated this activity after in vitro maturation. Taken together, the present report has delineated porcine blood DC and NIPC, permitting a more detailed understanding of innate immune defences, particularly in response to infections.
authors: Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland. artur.summerfield@ivi.admin.ch
authors: Artur
authors: A
authors: Summerfield
authors: Laurence
authors: L
authors: Guzylack-Piriou
authors: Alexander
authors: A
authors: Schaub
authors: Carlos P
authors: CP
authors: Carrasco
authors: Valerie
authors: V
authors: Tâche
authors: Bernard
authors: B
authors: Charley
authors: Kenneth C
authors: KC
authors: McCullough
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002470:Cell Survival; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016130:Immunophenotyping / Q000379:methods; D007372:Interferons / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D018613:Microscopy, Confocal / Q000379:methods; D009000:Monocytes / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D007372:Interferons