Intravenous administration of replication-incompetent adenovirus to rhesus monkeys induces thrombocytopenia by increasing in vivo platelet clearance.
A replication-incompetent adenovirus vector was administered to rhesus macaques at 1, 3 and 6 x 1012 particles/kg doses to investigate its toxicity. Platelet count decrements of 28%, 82% and 90%, respectively, were observed, with corresponding platelet half-lives of 69.0, 25.2 and 22.2 h (compared with 111 h in untreated animals). The platelet decline was equivalent for all three doses for 8 h, and platelet count recovery began as early as 8 h after infusion for low-dose recipients, or as late as 24 h for the medium and high dose recipients. These observations suggest that thrombocytopenia is a saturable, reversible consumptive process.
Wolins
N
N
Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA.
Lozier
J
J
Eggerman
T L
TL
Jones
E
E
Aguilar-Córdova
E
E
Vostal
J G
JG
D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000150:complications*; D000818:Animals; D001792:Blood Platelets / Q000821:virology*; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000008:administration & dosage*; D007275:Injections, Intravenous; D008253:Macaca mulatta; D010976:Platelet Count; D013921:Thrombocytopenia / Q000821:virology*