[Effect of chailing decoction and its active ingredients on experimental nephritis in rats].
OBJECTIVE
To screen the active ingredients of Chailing decoction (CLD) by using rat nephritis model induced by mono-colonal antibody 1-22-3 (mAb) injection.


METHODS
The active ingredients of CLD was screened by 5 successive times of experiment. In each time, 28 rats were randomly divided into 4 groups, 7 in each. Group 1 was treated with PBS as control, Groups 2-4 were treated separately with CLD and its various ingredients, the medication was started 5 days before and lasted to 8 days after modeling by peritoneal injection, 13 days totally. All the rats were killed 8 days after modeling to observe the effect of various drugs on proteinuria, morphological change of kidney and biochemical parameters.


RESULTS
CLD, Xiaochaihu decoction, various combination of thorowax root and its extract (saikosaponin-d) could reduce urinary protein, inhibit the proliferation of mesangial cell and expansion of extracellular matrix.


CONCLUSIONS
CLD and its active ingredients had inhibition on mAb induced rat model of nephritis, the active is saikosaponin-d.
Li
Ping
P
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029.
Li
Pei-heng
PH
Zhang
Zi-yi
ZY
D000818:Animals; D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D009393:Nephritis / Q000139:chemically induced / Q000473:pathology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011507:Proteinuria / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D