Postulation of and evidence for provirus existence in RSV-transformed cells and for an oncogenic activity associated with only part of the RSV genome.
This article gives a historical insight into the establishment of suitable models allowing the postulation that chicken Rous sarcoma virus (RSV) becomes integrated in different cells as a provirus. This is documented by the correspondence between two laboratories involved in these investigations. Special attention is paid to RSV-transformed mammalian cells, their virogenic nature, virus rescue by cell fusion, and finally their use for the oncogene v-src characterization. Two sets of experiments are mentioned, which provided an early indication of a transforming gene present in RSV.
Svoboda
J
J
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 37 Prague 6, Czech Republic. svoboda@img.cas.cz
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D002645:Chickens; D016679:Genome, Viral; D049673:History, 20th Century; D009858:Oncogenic Viruses / Q000235:genetics / Q000254:growth & development; D011533:Proviruses / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000266:history / Q000378:metabolism; D051381:Rats; D001357:Sarcoma, Avian / Q000235:genetics / Q000266:history / Q000821:virology
D012194:RNA-Directed DNA Polymerase