A selenium-containing single-chain abzyme with potent antioxidant activity.
Reactive oxygen species (ROS) are products of normal metabolic activities and are thought to be the cause of many diseases. A selenium-containing single-chain abzyme 2F3 (Se-2F3-scFv) that imitates glutathione peroxidase has been produced which has the capacity to remove ROS. To evaluate the antioxidant ability of Se-2F3-scFv, we constructed a ferrous sulfate/ascorbate (Vc/Fe2+)-induced mitochondrial damage model system and investigated the capacity of Se-2F3-scFv to protect mitochondria from oxidative damage. Se-2F3-scFv markedly decreased mitochondrial swelling, inhibited lipid peroxidation, and maintained the activity of cytochrome c oxidase, in comparison with Ebselen, a well-studied glutathione peroxidase mimic, indicating that Se-2F3-scFv has potential for treating diseases mediated by ROS.
You
Delin
D
Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P R China.
Ren
Xiaojun
X
Xue
Yan
Y
Luo
Guimin
G
Yang
Tongshu
T
Shen
Jiacong
J
D000818:Animals; D017151:Antibodies, Catalytic / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D003576:Electron Transport Complex IV / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress; D012643:Selenium / Q000378:metabolism*
D017151:Antibodies, Catalytic; D000975:Antioxidants; D005979:Glutathione Peroxidase; D003576:Electron Transport Complex IV; D012643:Selenium