title: [Effects of catecholamines on human preadipocyte proliferation and differentiation].
abstract: OBJECTIVE
  To study the effects of catecholamines on human preadipocyte proliferation and differentiation.
  
  
  METHODS
  Catecholamines (epinephrine, isoprenaline and noradrenaline) were added to the culture media of human preadipocytes. The proliferation of cells, the expression of GPDH and lipid droplet accumulation in cytoplasm were observed and recorded. The functions of alpha and beta receptors were examined with adding alpha and beta receptor blockers.
  
  
  RESULTS
  Epinephrine and isoprenaline stimulated human preadipocyte proliferation and inhibited GPDH up-regulation during differentiation. The three types of catecholamines inhibited lipid accumulation in cell differentiation. The beta-adrenoceptors played a key role during the process.
  
  
  CONCLUSIONS
  Human preadipocytes responded to catecholamine characteristically. The result would be applicable in the study of drugs for obesity.
authors: Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors: Xiao-hai
authors: XH
authors: Zhu
authors: Qing-lian
authors: QL
authors: He
authors: Zi-hao
authors: ZH
authors: Lin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D002395:Catecholamines / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D009765:Obesity / Q000188:drug therapy; D011943:Receptors, Adrenergic, beta; D015854:Up-Regulation
chemical_list: D002395:Catecholamines; D011943:Receptors, Adrenergic, beta; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine