NSF and p97/VCP: similar at first, different at last.
N-Ethylmaleimide sensitive factor (NSF) and p97/valosin-containing protein (VCP) are distantly related members of the ATPases associated with a variety of cellular activities (AAA) family of proteins. While both proteins have been implied in cellular morphology changes involving membrane compartments or vesicles, more recent evidence seems to imply that NSF is primarily involved in the soluble NSF attachment receptor (SNARE)-mediated vesicle fusion by disassembling the SNARE complex whereas p97/VCP is primarily involved in the extraction of membrane proteins. These functional differences are now corroborated by major structural differences based on recent crystallographic and cryo-electron microscopy studies. This review discusses these recent findings.
Brunger
Axel T
AT
Howard Hughes Medical Institute, and Department of Molecular and Cellular Physiology, and Stanford Synchrotron Radiation Laboratory, Stanford University, James H. Clark Center E300-C, 318 Campus Drive, Stanford, CA 94305-5432, USA. brunger@stanford.edu
DeLaBarre
Byron
B
D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D016384:Consensus Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D052066:N-Ethylmaleimide-Sensitive Proteins; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D000074405:Valosin Containing Protein; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D033921:Vesicular Transport Proteins; D000251:Adenosine Triphosphatases; D052066:N-Ethylmaleimide-Sensitive Proteins; C000614178:VCP protein, human; D000074405:Valosin Containing Protein