title: [The modified isolation and culture method of rat Sertoli cells].
abstract: OBJECTIVE
  To simplify the procedures of isolation of rat Sertoli cells and increase the yield of Sertoli cells cultured.
  
  
  METHODS
  Decapsulated rat testis from 16-22-day-old SD rats were digested by sequential two-step digestion using 0.25% pancreatin, 0.05% collagenase in order, and then the cell suspension was cultured in incubator under the condition of 37 degrees C and 5% CO2 for 48 h. The yields were observed and Fas Ligand (Fas-L) was tested by immunohistochemical method.
  
  
  RESULTS
  By the use of this modified method, the yielded sertoli cells accounted for 90% of all the cells harvested. High level expression of Fas-L was detected.
  
  
  CONCLUSIONS
  This modified method increases the yield of Sertoli cells and the procedures of isolation and culture are greatly simplified.
authors: Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Wei
authors: W
authors: Wang
authors: Mingqing
authors: M
authors: Xu
authors: Shu
authors: S
authors: Zhang
authors: Lünan
authors: L
authors: Yan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology*; D013737:Testis / Q000166:cytology*