Determination of gatifloxacin in human serum and urine by high-performance liquid chromatography with ultraviolet detection.
A simple and sensitive HPLC method for the determination of gatifloxacin concentrations in human serum and urine was developed and validated. Serum proteins were removed by ultrafiltration through a filtering device after adding a displacing agent. Urine samples were diluted with mobile phase prior to injection. Separation was achieved with a C18 reverse-phase column and gatifloxacin concentrations were determined using ultraviolet detection. The quantitation limits of the assay were 100 ng/ml in serum and 1.0 microg/ml in urine. The assay method was successfully applied to a pharmacokinetic study of gatifloxacin in healthy volunteers.
Overholser
Brian R
BR
Department of Pharmacy Practice, School of Pharmacy and Pharmacal Sciences, Purdue University, W7555 Myers Building, 1001 West 10th Street, Indianapolis, IN 46202-2879, USA.
Kays
Michael B
MB
Sowinski
Kevin M
KM
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D000077734:Gatifloxacin; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D024841:Fluoroquinolones; D000077734:Gatifloxacin