title: Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
abstract: Heteronuclear NMR relaxation measurements and hydrogen exchange data have been used to characterize protein dynamics in the presence or absence of stabilizing solutes from hyperthermophiles. Rubredoxin from Desulfovibrio gigas was selected as a model protein and the effect of diglycerol phosphate on its dynamic behaviour was studied. The presence of 100 mM diglycerol phosphate induces a fourfold increase in the half-life for thermal denaturation of D. gigas rubredoxin. A model-free analysis of the protein backbone relaxation parameters shows an average increase of generalized order parameters of 0.015 reflecting a small overall reduction in mobility of fast-scale motions. Hydrogen exchange data acquired over a temperature span of 20 degrees C yielded thermodynamic parameters for the structural opening reactions that allow for the exchange. This shows that the closed form of the protein is stabilized by an additional 1.6 kJ x mol(-1) in the presence of the solute. The results seem to indicate that the stabilizing effect is due mainly to a reduction in mobility of the slower, larger-scale motions within the protein structure with an associated increase in the enthalpy of interactions.
authors: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors: Pedro
authors: P
authors: Lamosa
authors: David L
authors: DL
authors: Turner
authors: Rita
authors: R
authors: Ventura
authors: Christopher
authors: C
authors: Maycock
authors: Helena
authors: H
authors: Santos
mesh_terms: D000577:Amides / Q000378:metabolism; D003901:Desulfovibrio / Q000737:chemistry*; D041961:Deuterium Exchange Measurement; D005994:Glycerophosphates / Q000494:pharmacology*; D006207:Half-Life; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation / Q000187:drug effects; D012416:Rubredoxins / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000577:Amides; D005994:Glycerophosphates; D012416:Rubredoxins; D012996:Solutions; C409185:diglycerol phosphate