Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6.
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.
Shin
I S
IS
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Kim
H R
HR
Nam
M J
MJ
Youn
H Y
HY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003208:Concanavalin A / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry*; D004285:Dogs / Q000097:blood / Q000235:genetics / Q000276:immunology*; D007377:Interleukin-3 / Q000737:chemistry / Q000235:genetics*; D015850:Interleukin-6 / Q000737:chemistry / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D007377:Interleukin-3; D015850:Interleukin-6; D021382:Protein Sorting Signals; D003208:Concanavalin A; D012313:RNA