title: Relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma.
abstract: OBJECTIVE
  To study relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma(HCC).
  
  
  METHODS
  Apoptotic rate (AR), proliferative index (PI) and S-phase fraction (SPF) were measured by flow cytometry, and p170, p21 and nucleoside diphosphate kinase (ndpk) by strept avidin-biotin complex immunohistochemical technique in 57 patients with HCC.
  
  
  RESULTS
  In this group, AR was 1.77%+/-0.19%, SPF 12.55%+/-0.68%, and PI 20.91%+/-1.12% (r=-0.173). p170, p21 and ndpk positive rates were 61.36%, 68.18%, 52.27% respectively in patients with a mean AR of </=1.77%, and 23.08%, 38.46%, 84.62% respectively in patients with a mean AR of >1.77% (all P<0.05). In patients with positive tumor invasiveness and metastasis, ndpk (+) was 43.75%, p21 (+) 75.00%, p170 (+) 65.63%, AR 1.12%+/-0.16%, PI 23.78%+/-1.48%, and SPF 13.90%+/-0.99%. In patients with negative invasiveness and metastasis, however, ndpk (+) was 80.00%, p21 (+) 44.00%, p170 (+) 36.00%, AR 2.32%+/-0.52%, PI 18.53%+/-0.82% and SPF 11.43%+/-0.70%.
  
  
  CONCLUSIONS
  Apoptosis of HCC is negatively correlated with its invasive and metastatic potential or other factors as proliferative activity, p21, p170 and ndpk.
authors: Department of Radiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. xiaogdk@sohu.com
authors: En-Hua
authors: EH
authors: Xiao
authors: Guo-Dong
authors: GD
authors: Hu
authors: Jin-Qing
authors: JQ
authors: Li
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology* / Q000556:secondary; D002455:Cell Division; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009701:Nucleoside-Diphosphate Kinase