title: Comparison of stem-cell-mediated osteogenesis and dentinogenesis.
abstract: The difference between stem-cell-mediated bone and dentin regeneration is not yet well-understood. Here we use an in vivo stem cell transplantation system to investigate differential regulation mechanisms of bone marrow stromal stem cells (BMSSCs) and dental pulp stem cells (DPSCs). Elevated expression of basic fibroblast growth factor (bFGF) and matrix metalloproteinase 9 (MMP-9, gelatinase B) was found to be associated with the formation of hematopoietic marrow in BMSSC transplants, but not in the connective tissue of DPSC transplants. The expression of dentin sialoprotein (DSP) specifically marked dentin synthesis in DPSC transplants. Moreover, DPSCs were found to be able to generate reparative dentin-like tissue on the surface of human dentin in vivo. This study provided direct evidence to suggest that osteogenesis and dentinogenesis mediated by BMSSCs and DPSCs, respectively, may be regulated by distinct mechanisms, leading to the different organization of the mineralized and non-mineralized tissues.
authors: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
authors: S
authors: S
authors: Batouli
authors: M
authors: M
authors: Miura
authors: J
authors: J
authors: Brahim
authors: T W
authors: TW
authors: Tsutsui
authors: L W
authors: LW
authors: Fisher
authors: S
authors: S
authors: Gronthos
authors: P Gehron
authors: PG
authors: Robey
authors: S
authors: S
authors: Shi
mesh_terms: D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D003239:Connective Tissue Cells / Q000502:physiology; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D003809:Dentin, Secondary / Q000254:growth & development; D003810:Dentinogenesis / Q000502:physiology*; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D006410:Hematopoiesis / Q000502:physiology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D010012:Osteogenesis / Q000502:physiology*; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins / Q000032:analysis; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D017154:Stromal Cells / Q000502:physiology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2; D020780:Matrix Metalloproteinase 9; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein