Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis.
OBJECTIVE
To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration.


METHODS
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.


METHODS
To develop DLK in rabbit eyes, 25 microL of lipopolysaccharide (LPS) solution at a concentration of 50 microg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed.


RESULTS
Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils.


CONCLUSIONS
The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.
Asano-Kato
Naoko
N
Minamiaoyama Eye Clinic, Tokyo, Japan. naokato@bc.iij4u.or.jp
Toda
Ikuko
I
Shimmura
Shigeto
S
Noda-Tsuruya
Tomoko
T
Fukagawa
Kazumi
K
Yoshinaga
Masaru
M
Matsukawa
Akihiro
A
Tsubota
Kazuo
K
D000818:Animals; D002465:Cell Movement; D003319:Corneal Stroma / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D016209:Interleukin-8 / Q000378:metabolism*; D007634:Keratitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D020731:Keratomileusis, Laser In Situ / Q000009:adverse effects*; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000473:pathology*; D011817:Rabbits; D012486:Salmonella typhimurium
D016209:Interleukin-8; D008070:Lipopolysaccharides