title: Evolution of aminoglycoside resistance phenotypes of four Gram-negative bacteria: an 8-year survey in a University Hospital in Greece.
abstract: In order to determine the resistance patterns and evolution trends of four common Enterobacteriaceae (Escherichia coli, Proteus spp., Klebsiella spp. and Enterobacter spp.), aminoglycoside resistance phenotypes of 8917 non-repetitive strains, isolated over an 8-year period, were analysed. Phenotypes were defined by examining the susceptibility of the strains to a panel of aminoglycosides, using disk diffusion method. A large diversity of different resistance phenotypes was encountered. A significant progressive increase in the proportions of wild-type E. coli strains was noted. Among resistant strains of Enterobacter spp. and Klebsiella spp., the incidence of phenotype KTANt (kanamycin, tobramycin, amikacin and netilmicin), indicative of AAC(6')-I production, was very high (66.7 and 46.5%, respectively). Phenotypes indicative for gentamicin-modifying enzymes as well as broad-spectrum combinations (combinations of gentamicin-modifying enzymes with AAC(6')-I) were infrequent.
authors: Departments of Clinical Bacteriology, University Hospital of Heraklion, Heraklion 1352, Crete 71110, Greece.
authors: I
authors: I
authors: Neonakis
authors: A
authors: A
authors: Gikas
authors: E
authors: E
authors: Scoulica
authors: A
authors: A
authors: Manios
authors: A
authors: A
authors: Georgiladakis
authors: Y
authors: Y
authors: Tselentis
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D019143:Evolution, Molecular; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D006115:Greece; D006801:Humans; D010641:Phenotype
chemical_list: D000617:Aminoglycosides