title: [Study on the effect of H2O2 against Acanthamoeba in vitro].
abstract: OBJECTIVE
  To detect the effect of H2O2 on Acanthamoeba spp..
  
  
  METHODS
  By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested.
  
  
  RESULTS
  At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites.
  
  
  CONCLUSIONS
  H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.
authors: State Key Laboratory of Bio-Orgnic and Natural Product Chemistry, Shanghai Institute of Orgnic Chemistry, Chinese Academy of Science, Shanghai 200032.
authors: Qun-fei
authors: QF
authors: Zhao
authors: Xue-liang
authors: XL
authors: Gao
authors: Min
authors: M
authors: Qian
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D015823:Acanthamoeba Keratitis / Q000469:parasitology; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D021261:Parasitic Sensitivity Tests
chemical_list: D000891:Anti-Infective Agents, Local; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase