title: A fluorescent carbazole derivative: high sensitivity for quadruplex DNA.
abstract: We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T(2)AG(3))(4). The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 microM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 microM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti-anti-anti-anti and anti-anti-syn-syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti-syn-anti-syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T(2)AG(3))(4) can be revealed by BMVC.
authors: Institute of Atomic and Molecular Sciences, Academia Sinica, PO Box 23-166, Taipei, 10764, Taiwan, Republic of China.
authors: Cheng-Chung
authors: CC
authors: Chang
authors: Jin-Yi
authors: JY
authors: Wu
authors: Chih-Wei
authors: CW
authors: Chien
authors: Wei-Sung
authors: WS
authors: Wu
authors: Heng
authors: H
authors: Liu
authors: Chi-Chih
authors: CC
authors: Kang
authors: Liang-Jye
authors: LJ
authors: Yu
authors: Ta-Chau
authors: TC
authors: Chang
mesh_terms: D000042:Absorption; D002227:Carbazoles / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D054856:G-Quadruplexes; D006801:Humans; D011726:Pyridinium Compounds / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D016615:Telomere / Q000235:genetics; D017186:Titrimetry
chemical_list: C486012:3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide; D002227:Carbazoles; D011726:Pyridinium Compounds; C041514:carbazole; D004247:DNA