title: Toluene diisocyanate exposure induces laryngo-tracheal eosinophilia, which can be ameliorated by supplementation with antioxidant vitamins in guinea pigs.
abstract: OBJECTIVE
  Toluene diisocyanate (TDI)-induced asthma is a common cause of occupational lung disease. In addition, a sore throat is one of the complaints of TDI-exposed workers. The aim of the present study was to determine whether TDI exposure induces laryngeal and/or tracheal lesions in experimental animals.
  
  
  METHODS
  Guinea pigs underwent naris application of TDI three times, and their respiratory tracts were then examined using light and electron microscopy. Some animals simultaneously received vitamins C and E. which function as antioxidant agents.
  
  
  RESULTS
  When TDI-treated animals showed the clinical sign of labored breathing, many eosinophils had appeared in the lamina propria and mucosa of both the larynx and trachea, which finally infiltrated the tract lumen through the ruptured epithelium. Laryngo-tracheal inflammation was more severe than that observed in the lungs. However, supplementation with antioxidant vitamins in TDI-treated animals ameliorated the respiratory eosinophilia.
  
  
  CONCLUSIONS
  Naris application of TDI induced laryngotracheitis. which was significantly suppressed by the antioxidant vitamins, This implies a preventive effect of the vitamins on this occupational disease.
authors: Department of Anatomy, Kagawa Medical University, Kagawa, Japan.
authors: He
authors: H
authors: Gu
authors: Masahiro
authors: M
authors: Itoh
authors: Nagahisa
authors: N
authors: Matsuyama
authors: Shogo
authors: S
authors: Hayashi
authors: Akira
authors: A
authors: Iimura
authors: Yoichi
authors: Y
authors: Nakamura
authors: Takanori
authors: T
authors: Miki
authors: Yoshiki
authors: Y
authors: Takeuchi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007830:Larynx / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D011657:Pulmonary Eosinophilia / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D014051:Toluene 2,4-Diisocyanate / Q000633:toxicity*; D014132:Trachea / Q000187:drug effects / Q000648:ultrastructure; D024502:alpha-Tocopherol / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D014051:Toluene 2,4-Diisocyanate; D024502:alpha-Tocopherol; D001205:Ascorbic Acid