Development of bespoke bioluminescent reporters with the potential for in situ deployment within a phenolic-remediating wastewater treatment system.
A suite of ecologically relevant, site-specific bioreporters was constructed by transposon mutagenesis of microorganisms isolated from a polluted phenolic-remediating wastewater treatment system. Four Pseudomonad species were engineered to carry a stable chromosomal copy of the lux operon (luxCDABE) derived from Photorhabdus luminescens. These recombinant reporter microorganisms were tested for bioluminescence response to relevant phenol concentrations in the laboratory and to phenolic-containing effluents generated by an industrial wastewater treatment plant. The reporters displayed proportional responses of bioluminescence decay with increasing phenol concentrations up to 800 mg l(-1) of phenol. When deployed against samples from the treatment system, they showed superior operational range and sensing capabilities to that observed for industry standard microorganisms such as Vibrio fischeri. Specifically, the engineered strains accurately predicted toxicity shifts in all the treatment compartments under study (with phenolic concentrations ranging from approximately 10 to 600 mg l(-1)) with a low coefficient of variation of replicate determinations (between 1.16% and 8.32%). This work highlights the utility of genetic modification of native microorganisms from sites of interest to provide robust and ecologically relevant organism-based reagents for toxicity monitoring with the potential for in situ deployment.
Wiles
Siouxsie
S
Molecular Microbial Ecology Laboratory, Centre for Ecology and Hydrology-Oxford, Mansfield Road, Oxford OX1 3SR, UK.
Whiteley
Andrew S
AS
Philp
Jim C
JC
Bailey
Mark J
MJ
D001673:Biodegradation, Environmental; D017930:Genes, Reporter; D005818:Genetic Engineering; D008163:Luminescent Measurements; D016254:Mutagenesis, Insertional; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D020637:Photorhabdus / Q000235:genetics / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D010636:Phenols; D014874:Water Pollutants, Chemical