title: Performance of the VERSANT HIV-1 resistance assays (LiPA) for detecting drug resistance in therapy-naive patients infected with different HIV-1 subtypes.
abstract: In this study we evaluated the performance of the VERSANT HIV-1 Resistance Assays (LiPA) in detecting drug resistance in therapy-naive HIV-infected patients diagnosed in Belgium in 2000. We compared the results with population sequencing and found concordance to be in line with previous studies in treatment-experienced patients (86.87% for reverse transcriptase (RT); 92.77% for protease (PRO)). Discordance was mainly due to indeterminate reactions on LiPA (8.45% for RT; 6.85% for PRO) and minor discordances (4.13% for RT; 0.25% for PRO). Major discordances were rare (0.46% for RT; 0.12% for PRO). Indeterminate reactions were significantly associated with strains belonging to non-B subtypes.
authors: Rega Institute for Medical Research and University Hospitals, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000, Leuven, Belgium.
authors: Inge
authors: I
authors: Derdelinckx
authors: Kristel
authors: K
authors: Van Laethem
authors: Bart
authors: B
authors: Maes
authors: Yoeri
authors: Y
authors: Schrooten
authors: Kirsten
authors: K
authors: De Schouwer
authors: Stéphane
authors: S
authors: De Wit
authors: Katrien
authors: K
authors: Fransen
authors: Sergio
authors: S
authors: García Ribas
authors: Michel
authors: M
authors: Moutschen
authors: Dolores
authors: D
authors: Vaira
authors: Georges
authors: G
authors: Zissis
authors: Marc
authors: M
authors: Van Ranst
authors: Eric
authors: E
authors: Van Wijngaerden
authors: Anne Mieke
authors: AM
authors: Vandamme
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001530:Belgium / Q000453:epidemiology; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D019380:Anti-HIV Agents; D054303:HIV Reverse Transcriptase; D016333:HIV Protease