title: Leukocyte adhesion to cold-preserved rat endothelial cells: role of actin disassembly and ICAM-1.
abstract: Leukocyte adhesion on reperfusion is a critical component of cold preservation injury, and involves increased intercellular adhesion molecule 1 (ICAM-1) expression on sinusoidal endothelial cells (SEC). This study determined whether ICAM-1 expression occurs during cold preservation and whether actin disassembly is necessary for ICAM-1 expression and leukocyte adhesion. ICAM-1 expression was measured in isolated rat SEC during 8 hours of cold preservation by immunofluorescence techniques. Leukocyte adhesion to cold-preserved SEC was measured in an assay using fluorescently labeled leukocytes. The calpain inhibitors N-acetyl-leu-leu-norleucinal/N-acetyl-leu-leu-methioninal and the actin stabilizer phalloidin were added in some studies to prevent actin disassembly. Cold-exposed SEC showed a rapid increase of surface ICAM-1 expression, reaching maximum values in 1 hour. Studies in permeabilized cells suggested that ICAM-1 moved from a perinuclear location to the cell surface. Actin stabilization had no effect on the time-dependent increase in ICAM-1 expression, but seemed to affect the distribution of ICAM-1 on the cell surface. Leukocyte adhesion to SEC correlated with ICAM-1 expression and was reduced to control levels by an anti-ICAM-1 antibody. Although actin stabilization did not reduce ICAM-1 expression, it did reduce leukocyte-SEC adhesion to control values. Increased ICAM-1 expression on cold-preserved SEC is a direct effect of cold. It is not related to actin disassembly, although it seems that actin disassembly affects the distribution of ICAM-1. Leukocyte adhesion to cold-preserved SEC requires both increased ICAM-1 and actin disassembly. Agents that inhibit actin disassembly can significantly decrease leukocyte adhesion regardless of increased ICAM-1 expression.
authors: Department of Surgery, Washington University in St. Louis, St. Louis, MO, USA.
authors: Stefan A
authors: SA
authors: Topp
authors: Gundumi A
authors: GA
authors: Upadhya
authors: Steven M
authors: SM
authors: Strasberg
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors; D015925:Cryopreservation; D004727:Endothelium / Q000166:cytology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D008856:Microscopy, Fluorescence; D010590:Phalloidine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D000199:Actins; D018799:Intercellular Adhesion Molecule-1; D010590:Phalloidine; D002154:Calpain; D002118:Calcium