Production of 5-aminolevulinic acid by an Escherichia coli aminolevulinate dehydratase mutant that overproduces Rhodobacter sphaeroides aminolevulinate synthase.
Two Escherichia coli strains, a wild-type strain and a hemB mutant, which contained the hemA gene from Rhodobacter sphaeroides produced aminolevulinate at 4 g l(-1). The hemB mutant did not increase the accumulation of aminolevulinate, an observation attributed to its 50% lower aminolevulinate synthase activity in than the wild-type. Growth was limited for both strains probably due to the accumulation of 0.5 g aminoacetone l(-1).
Xie
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Center for Molecular BioEngineering, Driftmier Engineering, University of Georgia, Athens, GA 30602, USA.
Eiteman
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MA
Altman
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D000624:5-Aminolevulinate Synthetase / Q000235:genetics / Q000378:metabolism*; D000622:Aminolevulinic Acid / Q000378:metabolism*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D000623:Porphobilinogen Synthase / Q000172:deficiency*; D011994:Recombinant Proteins / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase; D000623:Porphobilinogen Synthase