[Localization of proline-rich peptide (galarmin) in brain structures of rats (immunohistochemical study)].
In the present study the immunohistochemical localization of proline-rich peptide, so called galarmin, was examined in the brain structures of intact and galarmin-treated rats. Galarmin (a fragment of neurophysin II C-end glycopeptide) was isolated by A.A. Galoyan and coworkers in 1997, from the neurosecretory granules of bovine neurohypophysis, produced by the hypothalamic magnocellular nuclei. In intact rats galarmin-immunoreactive neurons and nerve fibers were widely distributed in the central nervous system. Single intramuscular injection of galarmin to the rats resulted in the increase of both galarmin-immunoreactivity and the number of galarmin-immunoreactive nerve cells, fibers and capillaries. In control experiments where the antisera against the fragment of immunophilin (a receptor of immunosuppressor macrolide FK-506) and the pancreatic neuropeptide Y were used as the primary antibodies, the significant increase of neuropeptide Y-immunoreactive nerve fibers and immunophilin-positive lymphocytes was revealed in galarmin-treated rats. Based on these results and the data on the motoneurons regeneration in the spinal cord hemisectioned rats given galarmin daily for 3 weeks, galarmin has been suggested to act as an immunomodulator, neurotransmitter and neuroregulator.
Abramian
S S
SS
Department of Neurohormones Biochemistry, National Academy of Sciences, Yerevan, Republic of Armenia.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009412:Nerve Fibers / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009481:Neurophysins / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D051381:Rats; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000276:immunology
D009478:Neuropeptide Y; D009481:Neurophysins; D010446:Peptide Fragments; D011392:Proline