Integrated bio-fluorescence sensor.
Due to the recent explosion in optoelectronics for telecommunication applications, novel optoelectronic sensing structures can now be realized. In this work, we explore the integration of optoelectronic components towards miniature and portable fluorescence sensors. The integration of these micro-fabricated sensors with microfluidics and capillary networks may reduce the cost and complexity of current research instruments and open up a world of new applications in portable biological analysis systems. A novel optoelectronic design that capitalizes on current vertical-cavity surface-emitting laser (VCSEL) technology is explored. Specifically, VCSELs, optical emission filters and PIN photodetectors are fabricated as part of a monolithically integrated near-infrared fluorescence detection system. High-performance lasers and photodetectors have been characterized and integrated to form a complete sensor. Experimental results show that sensor sensitivity is limited by laser background. The laser background is caused by spontaneous emission emitted from the side of the VCSEL excitation source. Laser background will limit sensitivity in most integrated sensing designs due to locating excitation sources and photodetectors in such close proximity, and methods are proposed to reduce the laser background in such designs so that practical fluorescent detection limits can be achieved.
Thrush
Evan
E
Solid State and Photonics Laboratory, Stanford University, Stanford, CA 94305, USA. ethrush@stanford.edu
Levi
Ofer
O
Ha
Wonill
W
Wang
Ke
K
Smith
Stephen J
SJ
Harris
James S
JS
D015374:Biosensing Techniques; D005453:Fluorescence; D007834:Lasers; D012680:Sensitivity and Specificity