Effect of Na+,K+,2Cl- cotransport inhibitor bumetanide on electrical and contractile activity of smooth muscle cells in guinea pig ureter.
The effect of Na(+),K(+),2Cl(-) cotransport inhibitor bumetanide on action potentials and contractions of smooth muscle cells in the ureter of guinea pigs evoked by electrical stimulation was studied by the method of double sucrose bridge. Bumetanide (10-100 microM) dose-dependently suppressed action potential and contractions of smooth muscle cells induced by 1-10 microM histamine, 10 microM mesatone, 5 mM tetraethylammonium, and 100 microM sodium nitroprusside. Our findings suggest that test substances modulate Na(+),K(+),2Cl(-) cotransport in smooth muscle cells.
Kovalev
I V
IV
Department of Biophysics, Siberian Medical University, Tomsk; Research Center, University of Montreal, Canada. Kovalev@mail.ru
Baskakov
M B
MB
Anfinogenova
Ya D
YD
Borodin
Yu L
YL
Kilin
A A
AA
Minochenko
I L
IL
Popov
A G
AG
Kapilevich
L V
LV
Medvedev
M A
MA
Orlov
S N
SN
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002034:Bumetanide / Q000494:pharmacology*; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D049994:Sodium Potassium Chloride Symporter Inhibitors; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D014513:Ureter / Q000033:anatomy & histology* / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology
D026902:Potassium Channel Blockers; D049994:Sodium Potassium Chloride Symporter Inhibitors; D028021:Sodium-Potassium-Chloride Symporters; D014665:Vasodilator Agents; D002034:Bumetanide; D009599:Nitroprusside; D019789:Tetraethylammonium