[Dynamic effect of low frequency complex impulse current on transdermal absorption of secretio bufonis].
OBJECTIVE
To explore the regulation of transdermal absorption of Secretio Bufonis (SB) and the effect of low frequency complex impulse current (LFCIC) on it.


METHODS
By modifying three-chamber flow diffusion pool to develop a prototype LFCIC device for transdermal delivery, using high performance liquid chromatograph (HPLC) to determine the quantitative transdermal absorption of the amount of ingredients of SB, including bufalin, cinobufagin and resibufogenin, etc. and the transdermal absorption velocity was calculated.


RESULTS
The chief ingredients of SB could be absorbed through skin, but the volume was low. Additional application of LFCIC could enhance the cumulative infiltration volume and velocity of transdermal diffusion. Difference appeared 2 hrs after and significant difference appeared 4 hrs after the application, and 13.8 Hz showed the optimal effect of transdermal delivery.


CONCLUSIONS
Chief ingredients of SB could be absorbed through transdermal medication, and LFCIC can evidently enhance the amount and velocity of transdermal absorption of SB.
Su
Yong-hua
YH
Beijing University of Chinese Medicine, Beijing 100029.
Niu
Xin
X
Yang
Xue-zhi
XZ
D000818:Animals; D002018:Bufanolides / Q000737:chemistry / Q000493:pharmacokinetics*; D002025:Bufonidae; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D007478:Iontophoresis; D008297:Male; D008419:Materia Medica; D011817:Rabbits; D012869:Skin Absorption
D002018:Bufanolides; D008419:Materia Medica; C002471:cinobufagin; C022777:bufalin