Detection of Helicobacter pylori and its CagA gene in tonsil and adenoid tissues by PCR.
OBJECTIVE
To determine the presence of Helicobacter pylori and, if detected, the prevalence of the CagA gene in adenotonsillectomy specimens by polymerase chain reaction (PCR).


METHODS
A prospective clinical trial.


METHODS
Tertiary referral center.


METHODS
The study population comprised 23 patients who had undergone adenoidectomy, tonsillectomy, or adenotonsillectomy under local or general anesthesia. Helicobacter pylori DNA was extracted from 3-mm-diameter tissue samples obtained from each tonsil and adenoid tissue specimens. The amplifications were performed for the 16S ribosomal RNA (rRNA) and CagA genes of H pylori in the samples of which H pylori DNA was detected.


RESULTS
In examining all the samples, 7 (30%) of 23 patients were shown to be positive for H pylori DNA, 5 (71%) of whom also possessed the CagA gene.


CONCLUSIONS
Tonsil and adenoid tissues may be an ecological niche of the mouth without regard to transient or permanent colonization. Oral-oral transmission may be a possible mode of spread of H pylori.
Cirak
Meltem Yalinay
MY
School of Medicine, Department of Microbiology and Clinical Microbiology, Gazi University, Ankara, Turkey. meltemyc@gazi.edu.tr
Ozdek
Ali
A
Yilmaz
Dicle
D
Bayiz
Unal
U
Samim
Erdal
E
Turet
Sevgi
S
D000234:Adenoids / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D014066:Palatine Tonsil / Q000382:microbiology*; D016133:Polymerase Chain Reaction
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori