[The antagonistic effect on anti-thy-1 serum-induced nephritis of rats injected by decorin-transfected mesangial cells vector].
OBJECTIVE
To inject decorin-transfected mesangial cells (MsC) vector into the kidneys of rats with anti-thy-1 serum-induced nephritis via left renal artery and observe the survival condition of MsC vector and its influence on glomerular lesions in rats with anti-thy-1 serum induced nephritis.


METHODS
Rat mesangio-proliferative glomerulonephritis was established by tail intravenous injection with rabbit anti-thy-1 serum (ATS). Decorin-transfected MsC was injected into rat kidneys via left renal artery. Primary culture, immunostaining for BrdU and decorin of transfected MsC lines were performed to observe their survival. Immunohistochemistry with image analysis was performed to detect the expression of BrdU, alpha-SMA, decorin, TGF-beta1, FN and ColIV in diseased glomeruli.


RESULTS
Rat anti-thy-1 serum-induced nephritis identified by pathological examination was successfully established by injecting rabbit ATS, and decorin transfected MsC vector was transfused to rat glomeruli via left renal artery. The active growth and positive expressions of BrdU and decorin proteins on the nuclei and cytoplasms of ex vivo MsC were observed respectively. TGF-beta1, FN, ColIV expressions in diseased glomeruli of rats with ATS nephritis were decreased significantly at day 4 (TGF-beta1, P < 0.05) and day 2 (FN and ColIV, P < 0.01) respectively, compared to uninjected kidneys.


CONCLUSIONS
MsC vector is successfully transferred to the glomeruli of experimental rats via left renal artery injection with no affect on cell survival. Decorin protein is expressed on the transfected MsC and shows antagonistic effect on the glomerular lesions of ATS rats. It suggests that the use of ex vivo MsC vector system can provide useful experimental basis for gene therapy of kidney disease in animal model.
Wang
Hui-jun
HJ
Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhang
Zhi-gang
ZG
Liu
Xue-guang
XG
Zhang
Xiu-rong
XR
Chen
Qi
Q
Guo
Mu-yi
MY
D000818:Animals; D058575:Decorin; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins; D015316:Genetic Therapy; D005920:Glomerular Mesangium / Q000378:metabolism*; D015432:Glomerulonephritis, Membranoproliferative / Q000473:pathology / Q000628:therapy*; D007106:Immune Sera / Q000276:immunology; D007678:Kidney Glomerulus / Q000473:pathology; D011509:Proteoglycans / Q000235:genetics*; D051381:Rats; D018800:Thy-1 Antigens / Q000276:immunology*; D014162:Transfection
C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D007106:Immune Sera; D011509:Proteoglycans; D018800:Thy-1 Antigens