Treadmill exercise increases cell proliferation in hippocampal dentate gyrus in alcohol-intoxicated rats.
OBJECTIVE
Effect of treadmill exercise on hippocampal cell proliferation under normal conditions has been well documented; however, this effect under alcohol intoxication conditions is not clarified, yet. In the present study, the effect of treadmill exercise on cell proliferation in the dentate gyrus in alcohol-intoxicated rats was investigated.


METHODS



METHODS
comparative investigation on number of 5-bromo-2'-deoxyuridine (BrdU)-positive cells in the dentate gyrus 8 days after commencement.


METHODS
animal laboratory.


METHODS
male Sprague-Dawley rats of 5 weeks in age weighing 150+/-10 g.


METHODS
animals were divided into 4 groups: the control-rest group, the control-exercise group, the alcohol-treated-rest group, and the alcohol-treated-exercise group. Animals of the alcohol-treated groups were injected intraperitoneally with alcohol (2 g/kg) once a day for 3 days. All animals were injected BrdU (50 mg/kg) intraperitoneally, and rats of exercise groups were made to run on treadmill for 30 min each day for 5 days following alcohol administration.


METHODS
mean number of BrdU-positive cells in dentate gyrus was observed via immunohistochemistry.


RESULTS
Treadmill exercise significantly increased the number of BrdU-positive cells in the dentate gyrus. Also, treatment with alcohol for 3 days inhibited cell proliferation and treadmill exercise alleviated alcohol-induced inhibition of new cell formation.


CONCLUSIONS
These results suggest the possibility that treadmill exercise may help in improvement following alcohol-induced brain damage.
Yu
B K
BK
Department of Physical Therapy, Shingu College, Kyonggi, Korea.
Yoon
B C
BC
Kim
S S
SS
Chun
S L
SL
Kim
E H
EH
Kim
K M
KM
Lim
B V
BV
Jang
M H
MH
Chung
J H
JH
Kim
C J
CJ
D000435:Alcoholic Intoxication / Q000097:blood / Q000473:pathology* / Q000503:physiopathology*; D000704:Analysis of Variance; D000818:Animals; D018891:Dentate Gyrus / Q000473:pathology*; D000431:Ethanol / Q000097:blood; D005080:Exercise Test; D006624:Hippocampus / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
D000431:Ethanol