title: [Investigation of p53 gene mutations in keloids using PCR-SSCP].
abstract: OBJECTIVE
  To detect gene mutations of p53 gene (exon 4-6) in fibroblasts.
  
  
  METHODS
  Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing.
  
  
  RESULTS
  Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids.
  
  
  CONCLUSIONS
  Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.
authors: Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors: Yong-bo
authors: YB
authors: Liu
authors: Jian-hua
authors: JH
authors: Gao
authors: Hong-jie
authors: HJ
authors: Duan
authors: Xiao-jun
authors: XJ
authors: Liu
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D005347:Fibroblasts; D016158:Genes, p53; D006801:Humans; D007627:Keloid / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53