Molecular diversity of Moraxella bovis isolated from Brazil, Argentina and Uruguay over a period of three decades.
The molecular profile of 30 Moraxella bovis strains, recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001, was determined through randomly applied polymorphic DNA (RAPD) analysis. Molecular profiles of nine strains recovered after 1990 varied from those recovered before 1990. The profiles of 13 strains (48%) differed from those of three vaccinal strains extensively used since 1984 in Argentina and Uruguay. Eight Argentinean strains, one from Brazil and two from Uruguay had identical RAPD profiles. Strains belonging to different serogroups had identical RAPD profiles, demonstrating that this technique was not able to discriminate among strains with low cross-reactivity indices. RAPD may be helpful in the primary characterization of M. bovis strains, but it does not replace serological characterization.
Conceição
Fabricio Rochedo
FR
Centro de Biotecnologia, Universidade Federal de Pelotas, CP 354, 96010-900 Pelotas, Brazil.
Dellagostin
Odir Antônio
OA
Paolichi
Fernando
F
Leturia
Analia Cobo
AC
Gil Turnes
Carlos
C
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks / Q000662:veterinary; D014644:Genetic Variation; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016955:Moraxella bovis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D014583:Uruguay / Q000453:epidemiology
D004269:DNA, Bacterial