Physical polymer matrices based on affinity interactions between peptides and polysaccharides.
A rapidly forming polymer matrix with affinity-based controlled release properties was developed based upon interactions between heparin-binding peptides and heparin. Dynamic mechanical testing of 10% (w/v) compositions consisting of a 3:1 molar ratio of poly(ethylene glycol)-co-peptide (approximately 18,000 g/mol) to heparin (approximately 18,000 g/mol) revealed a viscoelastic profile similar to that of concentrated, large molecular weight polymer solutions and melts. In addition, the biopolymer mixtures recovered quickly following thermal denaturation and mechanical insult. These gel-like materials were able to sequester exogenous heparin-binding peptides and could release these peptides over several days at rates dependent on relative heparin affinity. The initial release rates ranged from 3.3% per hour for a peptide with low heparin affinity to 0.025% per hour for a peptide with strong heparin affinity. By altering the affinity of peptides to heparin, a series of peptides can be developed to yield a range of release profiles useful for controlled in vivo delivery of therapeutics.
Seal
Brandon L
BL
The Harrington Department of Bioengineering, Arizona State University, P.O. Box 879709, Tempe, Arizona 85287-9709, USA.
Panitch
Alyssa
A
D001672:Biocompatible Materials / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004548:Elasticity; D006493:Heparin / Q000008:administration & dosage*; D008422:Materials Testing; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D055672:Static Electricity; D013450:Sulfones / Q000737:chemistry; D014783:Viscosity
D001672:Biocompatible Materials; D004337:Drug Carriers; D010455:Peptides; D013450:Sulfones; D011092:Polyethylene Glycols; C009873:divinyl sulfone; D006493:Heparin