Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes.
The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.
Rauski
Aleksandra
A
Immunology Research Center Branislav Janković, Institute of Immunology and Virology Torlak, Belgrade, Serbia and Montenegro.
Kosec
Dusko
D
Vidić-Danković
Biljana
B
Plećas-Solarović
Bosiljka
B
Leposavić
Gordana
G
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes; D016827:CD8 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D008297:Male; D009929:Organ Size; D010641:Phenotype; D011419:Propidium / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology*; D011744:Pyrimidinones / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*
D000319:Adrenergic beta-Antagonists; D015704:CD4 Antigens; D016827:CD8 Antigens; C067361:CD8 receptor; D011744:Pyrimidinones; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2; D011419:Propidium; C003954:merocyanine dye; D011433:Propranolol