title: Serological characteristics of peanut allergy in children.
abstract: BACKGROUND
  Food challenge is considered an excellent clinical tool for the diagnosis of specific food allergy. However in the case of peanut allergy it may be difficult to perform because of the severity of the reactions. The quantitation of a specific immunoglobulin E (IgE) response to different peanut allergens could also contribute to the improvement of the diagnosis. We characterized the IgE response to a whole peanut protein extract and to Ara h 1 and Ara h 2 in different groups of patients classified according to the severity of their allergic reactions.
  
  
  METHODS
  Specific serum IgE were analyzed in 96 children by enzyme-linked immunosorbent assay tests using a whole protein extract or purified peanut proteins and anti-human IgE monoclonal antibodies labeled with acetylcholinesterase.
  
  
  RESULTS
  A parallel was observed between levels of peanut-specific IgE and the classification in five groups and subgroups of patients upon increasing severity of symptoms, especially within the group of highest severity. Moreover, the highest frequency of positive response and the highest levels of specific IgE were observed with whole peanut protein extract.
  
  
  CONCLUSIONS
  In a retrospective evaluation of peanut allergy in children, we have shown that quantitation of peanut-specific IgE could be used to avoid a food challenge particularly in the case of severe reactions. When compared to Ara h 1 and Ara h 2, whole peanut protein extract appeared to be the most appropriate allergen to perform the test.
authors: INRA-Laboratoire d'Immuno-Allergie Alimentaire, CEA-Saclay, Gif sur Yvette, France.
authors: H
authors: H
authors: Bernard
authors: E
authors: E
authors: Paty
authors: L
authors: L
authors: Mondoulet
authors: A W
authors: AW
authors: Burks
authors: G A
authors: GA
authors: Bannon
authors: J M
authors: JM
authors: Wal
authors: P
authors: P
authors: Scheinmann
mesh_terms: D055516:2S Albumins, Plant; D000293:Adolescent; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant; D002648:Child; D002675:Child, Preschool; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007223:Infant; D008297:Male; D008565:Membrane Proteins; D021183:Peanut Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology; D012189:Retrospective Studies; D012698:Serologic Tests / Q000379:methods; D012720:Severity of Illness Index
chemical_list: D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; C078642:Ara h II protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D007073:Immunoglobulin E