title: Action of trehalose on the preservation of Lactobacillus delbrueckii ssp. bulgaricus by heat and osmotic dehydration.
abstract: OBJECTIVE
  This work determines the efficiency of trehalose on the preservation by heat or osmotic drying of a strain of Lactobacillus delbrueckii ssp. bulgaricus. Cell recovery at different trehalose concentrations during drying correlated with the surface properties and osmotic response of cells after rehydration.
  
  
  RESULTS
  Bacteria were dried in the presence of glycerol, trehalose, sucrose at 70 degrees C and at 20 degrees C. Trehalose attenuates the loss of viability at 0.25 m. At this concentration, the osmotic response and zeta potential of the bacteria were comparable with the nondried ones.
  
  
  CONCLUSIONS
  Trehalose diminishes significantly the damage produced by dehydration both when the bacteria are dried by heating or subjected to osmotic dehydration. This effect appears related to the preservation of the permeability to water and the surface potential of the bacteria.
  
  
  CONCLUSIONS
  Dehydration occurring during heating or during osmosis appears to have similar effects. As dehydration-induced damage is in correlation with osmotic response recovery and is hindered or buffered by the presence of trehalose, it may be related to water eliminated from biological structures involved in water permeation.
authors: Laboratorio de Fisicoquímica de Membranas Lipídicas, Facultad de Farmacia y Bioquímica (UBA), Buenos Aires, Argentina.
authors: A
authors: A
authors: Gómez Zavaglia
authors: E
authors: E
authors: Tymczyszyn
authors: G
authors: G
authors: De Antoni
authors: E
authors: E
authors: Aníbal Disalvo
mesh_terms: D000818:Animals; D003681:Dehydration; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D006358:Hot Temperature; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008892:Milk / Q000382:microbiology; D009995:Osmosis / Q000187:drug effects; D011309:Preservation, Biological / Q000379:methods*; D014199:Trehalose / Q000494:pharmacology*
chemical_list: D014199:Trehalose