Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2.
Mammalian voltage-gated K+ channels are assemblies of pore-forming alpha-subunits and modulating beta-subunits. To operate correctly, Kv4 alpha-subunits in the heart and central nervous system require recently identified beta-subunits of the neuronal calcium sensing protein family called K+ channel-interacting proteins (KChIPs). Here, Kv4.2.KChIP2 channels are purified, integrity of isolated complexes confirmed, molar ratio of the subunits determined, and subunit valence established. A complex has 4 subunits of each type, a stoichiometry expected for other channels employing neuronal calcium sensing beta-subunits.
Kim
Leo A
LA
Department of Pediatrics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
Furst
Johannes
J
Butler
Margaret H
MH
Xu
Shuhua
S
Grigorieff
Nikolaus
N
Goldstein
Steve A N
SA
D000818:Animals; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D018999:Charybdotoxin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004594:Electrophysiology; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007477:Ions; D051605:Kv Channel-Interacting Proteins; D015221:Potassium Channels / Q000737:chemistry*; D024642:Potassium Channels, Voltage-Gated; D011485:Protein Binding; D051672:Shal Potassium Channels; D013997:Time Factors; D014982:Xenopus laevis
D002135:Calcium-Binding Proteins; D007477:Ions; C497018:KCND2 protein, human; C496752:KCNIP2 protein, human; D051605:Kv Channel-Interacting Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels; D018999:Charybdotoxin; D005990:Glycerol