Lysis of heart endothelial cells from donor origin by cardiac graft infiltrating cells.
Endothelial cells may be involved in the acute rejection of allografts. In the present study, graft infiltrating lymphoid cell lines were propagated from a heart graft at the time of histological diagnosis of rejection. The cell lines containing CD8+ cells lysed donor-derived BLCL and endothelial cells (EC) but not third party BLCL or random EC, suggesting that HLA antigens were recognized. The cell lines containing CD4+ cells only did not lyse any target cells. The lysis of EC without preincubation with gamma interferon (gIFN) indicated that the HLA antigens recognized were class I antigens. These results suggested that lysis of donor EC may be one of the mechanisms involved in rejection.
Jutte
N H
NH
Department of Internal Medicine I, Erasmus University Rotterdam/Academic Hospital Rotterdam, Dijkzigt, Rotterdam, The Netherlands.
van Batenburg
M H
MH
Daane
C R
CR
Heijse
P
P
Vaessen
L M
LM
Balk
A H
AH
Mochtar
B
B
Claas
F H
FH
Weimar
W
W
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D004730:Endothelium, Vascular / Q000473:pathology*; D016027:Heart Transplantation / Q000276:immunology / Q000473:pathology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II