Investigation of the photochemical properties and in vitro phototoxic potential of bumetanide.
The photophysical and photochemical properties of bumetanide, a sulfonamide diuretic drug, have been investigated and the photodegradation of the drug on exposure to UV-B and UV-A radiation monitored by fluorimetric and chromatographic (HPLC) methods. The main photoproducts were isolated by solid-phase extraction and liquid chromatographic procedures, and their structure elucidated by 1H-NMR and mass spectrometry. Bumetanide generates only extremely small quantities of singlet oxygen (1O2) upon irradiation in the presence of oxygen. The in vitro 3T3 N RU phototoxicity test yielded no evidence of phototoxic action.
Fiori
Jessica
J
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Ballardini
Roberto
R
Hrelia
Patrizia
P
Andrisano
Vincenza
V
Tarozzi
Andrea
A
Cavrini
Vanni
V
D000818:Animals; D041702:BALB 3T3 Cells; D002034:Bumetanide / Q000737:chemistry* / Q000633:toxicity*; D004232:Diuretics / Q000737:chemistry / Q000633:toxicity; D066298:In Vitro Techniques; D051379:Mice; D018462:Photobiology; D010777:Photochemistry; D014466:Ultraviolet Rays
D004232:Diuretics; D002034:Bumetanide