title: [Immunohistochemical demonstration of cyclins A, B, D1, D3 and E in hepatocellular carcinomas using tissue microarrays].
abstract: OBJECTIVE
  To investigate the expression of five kinds of cyclins in hepatocellular carcinoma (HCC) and their association with degree of tumor differentiation, metastasis and infection of hepatitis B virus (HBV).
  
  
  METHODS
  The HCC tissue microarrays were composed of those from 273 cases of HCC tissues, 144 surrounding-tumor liver tissues and 10 normal liver tissues obtained from autopsy. The diameter of each specimens on tissue microarrays was 2.0 mm. Immunohistochemistry was used to detect the expression of cyclin A, cyclin B, cyclin D1, cyclin D3 and cyclin E on HCC tissue microarrays. The association of the expression of these cyclins with the infection rate of HBV was also analyzed.
  
  
  RESULTS
  Three paraffin-embedded HCC tissue microarrays were successfully constructed, including 136, 143 and 148 tissue spots, respectively. The positive rates of cyclins in 273 cases of HCC were cyclin A 52.7%, cyclin B 45.4%, cyclin D1 35.9%, cyclin D3 44.3% and cyclin E 23.1%; while the figures in 144 surrounding-tumor tissues were 8.3%, 5.6%, 4.9%, 6.3% and 1.4%, respectively. In 10 normal liver tissues these cyclins exhibited negative staining, with the exception that cyclin D1 was positive in one case of normal liver tissue. The positive rate of cyclins in HCC were significant higher than those in surrounding-tumor liver tissues (P < 0.01), in HCC tissues with histological grade II and III, the cyclins expression were stronger than that in grade I (P < 0.05). The positive rates of cyclins, except cyclin A in HCC with portal vein invasion were higher than those without portal vein invasion (P < 0.01). Infection of HBV did not have significant relationship with the expression of cyclins (P > 0.05).
  
  
  CONCLUSIONS
  Cyclins in different cell cycles overexpressed at varied levels in hepatocellular carcinoma, and the increased expression of cyclins may shorten the tumor cell cycle phase, accelerate cell proliferation, and have a close relationship with HCC aggressiveness.
authors: Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. mhzhu2000@hotmail.com
authors: Ming-hua
authors: MH
authors: Zhu
authors: Can-rong
authors: CR
authors: Ni
authors: Zhi
authors: Z
authors: Zhu
authors: Fang-mei
authors: FM
authors: Li
authors: Shun-min
authors: SM
authors: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D019925:Cyclin A / Q000032:analysis; D019926:Cyclin B / Q000032:analysis; D019938:Cyclin D1 / Q000032:analysis; D056743:Cyclin D3; D019927:Cyclin E / Q000032:analysis; D016213:Cyclins / Q000032:analysis*; D006509:Hepatitis B / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000737:chemistry*
chemical_list: C534696:CCND3 protein, human; D019925:Cyclin A; D019926:Cyclin B; D056743:Cyclin D3; D019927:Cyclin E; D016213:Cyclins; D019938:Cyclin D1