title: Characterization of gdp1+ as encoding a GDPase in the fission yeast Schizosaccharomyces pombe.
abstract: We have isolated the gdp1+ gene from Schizosaccharomyces pombe coding for a membrane protein with guanosine diphosphatase (GDPase) activity, which is highly homologous to Golgi GDPases isolated from other yeast species. The gdp1+ product, Gdp1p, displays both GDPase and uridine diphosphatase (UDPase) activities in vitro, with a strong dependence for calcium and manganese cations. The observation of a defect in N-glycosylation of invertase in S. pombe Deltagdp1 cells together with the ability of gdp1+ to functionally complement the defective O-mannosylation of chitinase in Saccharomyces cerevisiae cells disrupted in the GDA1 gene (gdp1+ homolog), suggests a main role for Gdp1p in protein glycosylation in fission yeast.
authors: Department of Genetics and Microbiology, Faculty of Biology, Universidad de Murcia, 30071 Murcia, Spain.
authors: Raquel
authors: R
authors: Sánchez
authors: Alejandro
authors: A
authors: Franco
authors: Mariano
authors: M
authors: Gacto
authors: Vicente
authors: V
authors: Notario
authors: José
authors: J
authors: Cansado
mesh_terms: D006031:Glycosylation; D016296:Mutagenesis; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D011755:Pyrophosphatases; C023273:uridine diphosphatase; C010357:guanosine-diphosphatase