Beta-phenylpyruvate and glucose uptake in isolated mouse soleus muscle and cultured C2C12 muscle cells.
Previous investigation demonstrated the potential of beta-phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with beta-phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM beta-phenylpyruvate in soleus muscle (P<0.01) and C2C12 cells (P<0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in Vmax compared with controls (P<0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to beta-phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of beta-phenylpyruvate.
Ben-Abraham
Ron
R
Department of Anesthesiology, Tel Aviv Sourasky Medical Center, 64239 Tel Aviv, Israel.
Gazit
Vered
V
Vofsi
Oded
O
Ben-Shlomo
Izahar
I
Reznick
Abraham Z
AZ
Katz
Yeshayahu
Y
D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism*; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010667:Phenylpyruvic Acids / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D010667:Phenylpyruvic Acids; C495678:Slc2a1 protein, mouse; C495693:Slc2a4 protein, mouse; D005947:Glucose; C031606:phenylpyruvic acid