[Polymorphism analysis of cytochrome C oxidase 1 (COI) gene in Necator americanus collected from 5 provinces in China].
OBJECTIVE
To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates.


METHODS
COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced.


RESULTS
The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation occurred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the sequences ranged from 1.34% to 2.18%.


CONCLUSIONS
The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.
Li
Tie-hua
TH
Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025.
Guo
Xiang-rong
XR
Hu
Ling
L
Xiao
Shu-hua
SH
Xue
Hai-chou
HC
Hawdon
John
J
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid
D018509:DNA, Helminth; D003576:Electron Transport Complex IV