Strong telomerase activity of B lymphocyte from mesenteric lymph nodes of patients with inflammatory bowel disease.
Telomerase acitivity can be induced in human B lymphocytes by in vitro stimulation of their antigen receptors. To determine whether telomerase activity is induced in vivo, we analyzed telomerase activity in B lymphocytes from the mesenteric lymph nodes of patients with inflammatory bowel disease (IBD), whose lymph nodes are well known to be strongly stimulated, and from those of noninflamed controls. Seven IBD patients and 4 noninflamed controls were enrolled. Telomerase activity was assayed by telomeric repeat amplification protocol with minor modifications. The mesenteric lymph nodes from patients with IBD had stronger telomerase activity than those from controls or peripheral mononuclear cells. Isolation of CD19+ B lymphocytes from these lymph nodes showed that this strong activity resides in this lymphocytes subpopulation. This study provides the evidence that telomerase activity is induced in human B lymphocytes in human inflammatory disease.
Takagi
Sho
S
Division of Gastroenterology, Tohoku University Graduate School of Medicine, 1-1 Seiryo, Aoba, Sendai 980-8574, Japan. stakagi@int3.med.tohoku.ac.jp
Kinouchi
Yoshitaka
Y
Chida
Masaki
M
Hiwatashi
Nobuo
N
Noguchi
Mitsunori
M
Takahashi
Seiichi
S
Shimosegawa
Tooru
T
D000328:Adult; D001402:B-Lymphocytes / Q000201:enzymology*; D016022:Case-Control Studies; D004790:Enzyme Induction; D006367:HeLa Cells; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000201:enzymology*; D008198:Lymph Nodes / Q000201:enzymology*; D008643:Mesentery; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase