Change in electrophoretic mobility of PC12 cells after culturing on PVA membranes modified with different diamines.
The cell-biomaterial interaction is of extreme importance in regulating the numerous functions necessary for cell adhesion, growth, and differentiation. In the current study, electrophoresis was used to investigate the interactions between cells and biomaterials by measuring the change in electrophoretic mobility of pheochromocytoma (PC12) cells after they were cultured on the poly (vinyl alcohol) membranes modified with different diamines. Variations in cellular activity and electrophoretic mobility of cultured cells were compared. It was found that the intracellular metabolism and the cell surface charge properties were altered after cells contacting biomaterials and the variation of the latter occurred earlier than that of the former. Although the precise mechanism by which the variation of electrophoretic mobility of cultured PC12 cells was unknown, the biomaterials could influence the cell mobility within a short incubation time. It was hypothesized that changes in extracellular matrix components of cell surface may be in part responsible.
Young
Tai-Horng
TH
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan 100, ROC. thyoung@ha.mc.ntu.edu.tw
Hung
Chih-Huang
CH
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D003959:Diamines / Q000737:chemistry* / Q000378:metabolism; D004577:Electron Probe Microanalysis; D004586:Electrophoresis; D008422:Materials Testing; D016716:PC12 Cells; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013499:Surface Properties
D001672:Biocompatible Materials; D003959:Diamines; D011142:Polyvinyl Alcohol