[Synthesis of N-[2-(cholesteryloxycarbonylamino) ethyl] carbamoylmethylated mannan].
OBJECTIVE
To study the synthesis of chol-AECM-mannan.


METHODS
Using mannan as the main material, we synthesized a compound with polysaccharides partially modified by cholesterol (namely chol-AECM-mannan) through carboxymethylating, carbamoylating and condensation reaction.


RESULTS
The product was confirmed by IR spectrum and 1H nuclear magnetic resonance (1HNMR). The proton integral ratio of mannan and cholesterol is 100: 5, that is to say, the numbers of the substituted cholesterol per 100 mannose units are 1. 1. The production rate is 24%.


CONCLUSIONS
The method is a effective pathway in term of synthesizing chol-AECM-mannan.
Yang
Li
L
Key Laboratory of Biotherapy of Human Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Cheng
Li
L
Wei
Yuquan
Y
Tian
Ling
L
D002784:Cholesterol / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000138:chemical synthesis* / Q000737:chemistry; D013055:Spectrophotometry, Infrared
D008081:Liposomes; D008351:Mannans; C488215:N-(2-(cholesteryloxycarbonylamino)ethyl)carbamoylmethylated mannan; D002784:Cholesterol