title: Analysis of serotype AD strains from F1 progenies between urease-positive- and negative-strains of Cryptococcus neoformans.
abstract: Cryptococcus neoformans is a pathogenic basidiomycete with a defined sexual cycle involving mating between haploid yeast cells with a transient diploid state. We examined F1 progeny from a crossing between the urease-negative strain (environmental isolate, serotype A, mating type alfa, haploid) and a tester strain (B 3502 from NIH of USA; urease-positive, serotype D, mating type a, haploid) for serotype, mating type, ploidy and urease activity, and performed partial sequencing of the urease gene. Phenotypes of the F1 progeny and results of SSCP analyses suggested that the serotype AD strain of the F1 progeny is a hybrid of the parental serotype A and D strains.
authors: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors: Reiko
authors: R
authors: Tanaka
authors: Kazuko
authors: K
authors: Nishimura
authors: Yumi
authors: Y
authors: Imanishi
authors: Ichiro
authors: I
authors: Takahashi
authors: Yoko
authors: Y
authors: Hata
authors: Makoto
authors: M
authors: Miyaji
mesh_terms: D003455:Cryptococcus neoformans / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D012703:Serotyping; D014510:Urease / Q000032:analysis
chemical_list: D014510:Urease