title: Detection of p53 gene mutations by nonisotopic RNase cleavage assay as a predictor of poor prognosis in colorectal cancers.
abstract: The present study was undertaken to evaluate p53 gene mutation as a prognostic factor in patients with colorectal cancer. Nonisotopic RNase cleavage assay (NIRCA), recently used for detecting gene mutations, was employed to detect p53 gene mutations in this study. In 15 samples of colorectal tumors, NIRCA was confirmed to be simple, accurate, and thus useful for clinical use, compared with polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP). In another group of 79 cases of colorectal cancer analyzed for p53 gene mutation by using NIRCA, mutations were detected in 58 of 79 (73.4%) cases. Multivariate Cox proportional-hazards analysis showed that p53 gene mutation was a significant prognostic factor in patients with colorectal cancer. Our results showed that NIRCA is a simple and sensitive method, and thus useful for genetic screening of colorectal cancer. Furthermore, our results showed that p53 gene mutation is an independent predictor of poor prognosis in colorectal cancers.
authors: First Department of Surgery, Nagasaki University School of Medicine, 1-7-1, Sakamoto Machi, Nagasaki City, Nagasaki 852-8501, Japan. m-haseba@guitar.ocn.ne.jp
authors: Masatoshi
authors: M
authors: Haseba
authors: Shigekazu
authors: S
authors: Hidaka
authors: Takashi
authors: T
authors: Tsuji
authors: Hiroshi
authors: H
authors: Yano
authors: Hideaki
authors: H
authors: Komatsu
authors: Terumitu
authors: T
authors: Sawai
authors: Toru
authors: T
authors: Yasutake
authors: Tohru
authors: T
authors: Nakagoe
authors: Yutaka
authors: Y
authors: Tagawa
authors: Hiroyoshi
authors: H
authors: Ayabe
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D016016:Proportional Hazards Models; D012260:Ribonucleases / Q000737:chemistry*; D012680:Sensitivity and Specificity; D016019:Survival Analysis
chemical_list: D012260:Ribonucleases