Analysis of ginkgolides and bilobalide in Ginkgo biloba L. extract injections by high-performance liquid chromatography with evaporative light scattering detection.
A RP-HPLC method with evaporative light scattering detection (ELSD) was developed for the determination of ginkgolides and bilobalide in Ginkgo biloba L. extract injections. The samples were extracted with ethyl acetate and the resulting extract was purified by aluminum oxide column. The resultant solution was determined by HPLC on a C(18) column with methanol-water (33:67, v/v) as eluent. The optimum ELSD parameters were set. The recovery of the method was between 98.3 and 102.1%. The method is suitable for routine quantitation of terpenes in Ginkgo biloba L. extract injections.
Tang
Cui
C
State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
Wei
Xiuli
X
Yin
Chunhua
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003517:Cyclopentanes / Q000032:analysis* / Q000737:chemistry; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D005663:Furans / Q000032:analysis* / Q000737:chemistry; D020441:Ginkgo biloba; D046934:Ginkgolides; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry
D003517:Cyclopentanes; D004224:Diterpenes; D005663:Furans; D046934:Ginkgolides; D010936:Plant Extracts; C073710:bilobalide