Relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma.
OBJECTIVE
To study relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma(HCC).


METHODS
Apoptotic rate (AR), proliferative index (PI) and S-phase fraction (SPF) were measured by flow cytometry, and p170, p21 and nucleoside diphosphate kinase (ndpk) by strept avidin-biotin complex immunohistochemical technique in 57 patients with HCC.


RESULTS
In this group, AR was 1.77%+/-0.19%, SPF 12.55%+/-0.68%, and PI 20.91%+/-1.12% (r=-0.173). p170, p21 and ndpk positive rates were 61.36%, 68.18%, 52.27% respectively in patients with a mean AR of </=1.77%, and 23.08%, 38.46%, 84.62% respectively in patients with a mean AR of >1.77% (all P<0.05). In patients with positive tumor invasiveness and metastasis, ndpk (+) was 43.75%, p21 (+) 75.00%, p170 (+) 65.63%, AR 1.12%+/-0.16%, PI 23.78%+/-1.48%, and SPF 13.90%+/-0.99%. In patients with negative invasiveness and metastasis, however, ndpk (+) was 80.00%, p21 (+) 44.00%, p170 (+) 36.00%, AR 2.32%+/-0.52%, PI 18.53%+/-0.82% and SPF 11.43%+/-0.70%.


CONCLUSIONS
Apoptosis of HCC is negatively correlated with its invasive and metastatic potential or other factors as proliferative activity, p21, p170 and ndpk.
Xiao
En-Hua
EH
Department of Radiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. xiaogdk@sohu.com
Hu
Guo-Dong
GD
Li
Jin-Qing
JQ
D018435:ATP Binding Cassette Transporter, Subfamily B; D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology* / Q000556:secondary; D002455:Cell Division; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009701:Nucleoside-Diphosphate Kinase