title: [Inhibition of MCP-1 mRNA expression by propylene glycol mannate sulfate in hyperlipidemic rat aorta].
abstract: OBJECTIVE
  To study the effects of prophylene glycol mannate sulfate (PGMS) on monocyte chemoattractant protein-1 (MCP-1) mRNA expression in hyperlipidemic rat aorta and to clarify the molecular mechanism of PGMS for the prevention of atherosclerosis.
  
  
  METHODS
  PGMS (37.8 and 75.6 mg.kg-1.d-1, ig) or PGMS (37.8 and 75.6 mg.kg-1.d-1, ig) combined with diethyldithiocarbamate (DDC, an inhibitor of SOD, 200 mg.kg-1 every three days, i.p.) were given to hyperlipidemic rats for three weeks. The MDA content and SOD activity were determined after 12 h of starvation, and MCP-1 mRNA expression in aorta was detected by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  There was significant decrease (29.46% or 58.40)% of MCP-1 mRNA expression in aortic after the therapy. The SOD activity increased markedly and the MDA content decreased at the same time. After treatment with DDC, the SOD activity was inhibited and the MDA content increased, but with no significant effect on MCP-1 mRNA expression.
  
  
  CONCLUSIONS
  PGMS inhibited MCP-1 mRNA expression with no relation to its effect on decreasing MDA content.
authors: Institute of Marine Drugs and Foods, Ocean University of China, Qingdao 266003, China.
authors: Yan
authors: Y
authors: Gao
authors: Wen-gong
authors: WG
authors: Yu
authors: Feng
authors: F
authors: Han
authors: Xin-zhi
authors: XZ
authors: Lu
authors: Qian-hong
authors: QH
authors: Gong
authors: Hua-shi
authors: HS
authors: Guan
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006949:Hyperlipidemias / Q000097:blood / Q000473:pathology; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D011409:Propylene Glycols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D000960:Hypolipidemic Agents; D011409:Propylene Glycols; D012333:RNA, Messenger; D008315:Malondialdehyde; D013482:Superoxide Dismutase