title: Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows.
abstract: To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.
authors: College of Veterinary Medicine, Chonbuk National University, Chonju, Korea.
authors: Byeong K
authors: BK
authors: Baek
authors: Kim B
authors: KB
authors: Soo
authors: Jin H
authors: JH
authors: Kim
authors: Jin
authors: J
authors: Hur
authors: Bou O
authors: BO
authors: Lee
authors: J M
authors: JM
authors: Jung
authors: Misao
authors: M
authors: Onuma
authors: Anthony O
authors: AO
authors: Oluoch
authors: Chang-Hyun
authors: CH
authors: Kim
authors: Ibulaimu
authors: I
authors: Kakoma
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology / Q000635:transmission*; D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D016794:Theileria / Q000235:genetics / Q000302:isolation & purification*; D013801:Theileriasis / Q000175:diagnosis / Q000469:parasitology / Q000635:transmission*
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D016054:DNA, Protozoan