The first isolation of two types of trifluoroleucine resistant mutants of Saccharomyces servazzii.
Saccharomyces servazzii plays a crucial role in the making of Japanese radish pickles. To make more flavorsome pickles, we sought to generate trifluoroleucine-resistant mutants of S. servazzii. The three resulting mutants could be classified into two types: one that produces more isoamyl alcohol than the parental strain, and one that produces less. The first type has been well documented in Saccharomyces cerevisiae but the latter appears to be novel and has been characterized as such.
Tominaga
Tatsuya
T
Saitama Prefectural Industrial Technology Center North Institute, 2-133, Suehiro, Kumagayashi, Saitama 360-0031, Japan. tominaga@itc-n.pref.saitama.jp
Okuzawa
Yohei
Y
Kato
Shiro
S
Suzuki
Motofumi
M
D015079:2-Isopropylmalate Synthase / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D025141:Drug Resistance, Fungal / Q000502:physiology*; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009154:Mutation; D000439:Pentanols / Q000378:metabolism*; D012440:Saccharomyces / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D013045:Species Specificity
D000439:Pentanols; C035177:5',5',5'-trifluoroleucine; C029683:isopentyl alcohol; D015079:2-Isopropylmalate Synthase; D007930:Leucine