Characterisation of the guinea pig model of osteoarthritis by in vivo three-dimensional magnetic resonance imaging.
OBJECTIVE
To characterise longitudinal changes in joint integrity and cartilage volume in vivo in the guinea pig spontaneous osteoarthritis (OA) model by magnetic resonance imaging (MRI).


METHODS
Guinea pigs knee were imaged in vivo by high-resolution three-dimensional (3D) MRI between the ages of 3 and 12 months. Image analysis was performed to assess qualitative knee joint changes between 3 and 12 months (n=16) and quantitative volumetric changes of the medial tibial cartilage between 9 and 12 months (n=7). After imaging, animals were killed and knees were assessed macroscopically and histologically.


RESULTS
From 3 to 6 months qualitative observation by MRI and histopathology indicated localised cartilage swelling on the medial tibial plateau. At 6 months, bone cysts had developed in the epiphysis. At 9 months, we observed by MRI and histopathology, fragmentation of the medial tibial cartilage in areas not protected by the meniscus. Cartilage degeneration had intensified at 12 months with evidence of widespread loss of cartilage throughout the tibial plateau. Segmentation of the MR cartilage images showed a 36% loss of volume between 9 and 12 months.


CONCLUSIONS
We have achieved 3D image acquisition and segmentation of knee cartilage in a guinea pig model of chronic OA, which permits measurements previously only possible in man. High resolution and short acquisition time allowed qualitative longitudinal characterisation of the entire knee joint and enabled us to quantify for the first time longitudinal tibial cartilage volume loss associated with disease progression.
Tessier
J J
JJ
Enabling Science and Technology, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK. jean.tessier@astrazeneca.com
Bowyer
J
J
Brownrigg
N J
NJ
Peers
I S
IS
Westwood
F R
FR
Waterton
J C
JC
Maciewicz
R A
RA
D000818:Animals; D001845:Bone Cysts / Q000209:etiology / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006614:Hindlimb / Q000473:pathology; D007596:Joints / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D020370:Osteoarthritis, Knee / Q000150:complications / Q000473:pathology*; D013977:Tibia / Q000473:pathology; D013997:Time Factors