[cDNA cloning and sequence analysis of major allergen of Dermatophagoides farinae (Der f 2) in south China].
OBJECTIVE
To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China.


METHODS
cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed.


RESULTS
cDNA of Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes.


CONCLUSIONS
The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.
Hao
Min-qi
MQ
Guangzhou Institute of Respiratory Diseases, Guangzhou 510120.
Xu
Jun
J
Zhong
Nan-shan
NS
D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics*; D060829:Arthropod Proteins; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D018076:DNA, Complementary; C451160:Dermatophagoides farinae antigen f 2