Investigation of the antigen antibody reaction between anti-bovine serum albumin (a-BSA) and bovine serum albumin (BSA) using piezoresistive microcantilever based sensors.
A new microsensor application based on piezoresistive microcantilever technology has been used to study the interaction of anti-bovine serum albumin (a-BSA) with bovine serum albumin (BSA). A thin layer of BSA attached to a glass slide was used as the active sensing layer for the detection of a-BSA in solution. This design produced a large, consistent cantilever deflection when exposed to the analyte. In this system, the cantilever deflection is measured as a simple resistance change in the piezoresistive channel within the cantilever. In a second set of experiments, 3:1 BSA:PEO protein/polymer blended substrates were used as the active sensing layer for the detection of a-BSA in an aerosol delivery. A distinct signature for the analyte, separate from the water vapor carrier, is obtained for this system.
Kooser
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A
Department of Chemistry, Northern Arizona University, Flagstaff, AZ 86011, USA.
Manygoats
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Eastman
M P
MP
Porter
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TL
D000336:Aerosols / Q000032:analysis*; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D017097:Electric Impedance; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*
D000336:Aerosols; D000906:Antibodies; D000936:Antigen-Antibody Complex; D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine