Lyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments.
Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise and resuscitate the sporozoites of T. parva. Sporozoites survived lyophilisation and were effective for immunisation. Lyophilised stabilate survived for 2 weeks at 5 degrees C and for 12 weeks at -20 degrees C. Although the viability of the stabilates was severely reduced during lyophilisation, this work suggests that this method has potential and should be considered for other Apicomplexan parasites such as Babesia sp. or Plasmodium sp.
Marcotty
T
T
Department of Veterinary Medicine, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium. tmarcotty@itg.be
Berkvens
D
D
Besa
R K
RK
Losson
B
B
Dolan
T T
TT
Madder
M
M
Chaka
G
G
Van den Bossche
P
P
Brandt
J
J
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D005612:Freeze Drying; D016052:Protozoan Vaccines / Q000276:immunology; D034101:Sporozoites / Q000276:immunology*; D013696:Temperature; D016797:Theileria parva / Q000276:immunology*; D013801:Theileriasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control
D016052:Protozoan Vaccines