title: Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus interacts with human myeloid cell nuclear differentiation antigen induced by interferon alpha.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpes virus type 8 (HHV-8) is tightly linked to the development of Kaposi's sarcoma, primary effusion lymphoma (PEL) and some cases of multicentric Castleman's disease. Latency-associated nuclear antigen (LANA) is one of a limited number of KSHV genes consistently expressed in these diseases as well as in KSHV-infected cell lines derived from PEL, and has been shown to play crucial role in persistence of KSHV genomes in the infected cells. In this study, we explored the cellular factors that interact with LANA using yeast two-hybrid screening, and isolated a part of gene encoding human myeloid cell nuclear differentiation antigen (MNDA). MNDA is a hematopoietic interferon-inducible nuclear proteins with a HIN-200 family member with conserved 200-amino acid repeats. Immunoprecipitation assay revealed that LANA interacted with MNDA in a mammalian embryonic kidney cell line. MNDA transcript was undetectable in three PEL cell lines by reverse-transcription polymerase chain reaction, but it was induced by interferon alpha (IFNalpha). Moreover, LANA and MNDA were co-localized in the nuclei of MNDA-expressing PEL cells. Our results suggest that LANA interacts with MNDA in KSHV-infected cells exposed to IFNalpha. Such interaction may modulate IFN-mediated host defense activities.
authors: Division of Virology, Niigata University Graduate School of Medical and Dental Sciences, 1-757, Asahimachi-Dori, Niigata, 951-8510, Japan.
authors: Masaya
authors: M
authors: Fukushi
authors: Masaya
authors: M
authors: Higuchi
authors: Masayasu
authors: M
authors: Oie
authors: Takafumi
authors: T
authors: Tetsuka
authors: Francis
authors: F
authors: Kasolo
authors: Kouji
authors: K
authors: Ichiyama
authors: Naoki
authors: N
authors: Yamamoto
authors: Harutaka
authors: H
authors: Katano
authors: Tetsutaro
authors: T
authors: Sata
authors: Masahiro
authors: M
authors: Fujii
mesh_terms: D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000956:Antigens, Viral / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011994:Recombinant Proteins; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000821:virology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D015214:Antigens, Differentiation, Myelomonocytic; D000956:Antigens, Viral; D004279:DNA, Viral; D007370:Interferon Type I; C075373:MNDA protein, human; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C109540:latency-associated nuclear antigen