title: [Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].
abstract: OBJECTIVE
  To improve SEA-ELISA, an immunodiagnostic assay for schistosomiasis.
  
  
  METHODS
  Soluble egg antigen (SEA) of Schistosoma japonicum was treated with sodium periodate (SP) in order to oxidate its glycosylated epitopes. ELISA using the treated SEA was then performed to detect specific antibodies to SEA in the sera of schistosomiasis patients.
  
  
  RESULTS
  Serum samples were tested by ELISA using SEA treated with sodium periodate (SP-SEA-ELISA), including 64 sera from cases with chronic schistosomiasis japonica, 119 sera from normal individuals in non-endemic area, 34 sera from patients with clonorchiasis, 33 sera of paragonimiasis cases and 36 sera from patients with cysticercosis. The results showed that its sensitivity (98.4%) was similar to that of the routine SEA-ELISA (100.0%) (P > 0.05) and the specificity is higher than that of the SEA-ELISA (P < 0.05). SP-SEA-ELISA showed a higher negative rate (89.0%) for sera of schistosomiasis patients 12 months post-treatment than that of the SEA-ELISA (42.1%).
  
  
  CONCLUSIONS
  Use of SP-SEA can increase the specificity of ELISA, reduce cross-reactivity with serum samples from cases infected with other parasites and improve its value in evaluating therapeutic efficacy.
authors: Department of Parasitology, Xiangya Medical College Central South University, Changsha 410078.
authors: Yue-long
authors: YL
authors: Huang
authors: Xin-yuan
authors: XY
authors: Yi
authors: Xian-fang
authors: XF
authors: Zeng
authors: Ran
authors: R
authors: Zhang
authors: Shi-shan
authors: SS
authors: Yuan
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000918:Antibody Specificity; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010504:Periodic Acid; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010504:Periodic Acid; C009288:metaperiodate