title: Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana.
abstract: Cytokinins have been implicated in developmental and growth processes in plants including cell division, chloroplast biogenesis, shoot meristem initiation and senescence. The regulation of these processes requires changes in cytokinin-responsive gene expression. Here, we induced the expression of a bacterial isopentenyl transferase gene, IPT, in transgenic Arabidopsis thaliana seedlings to study the regulation of genome-wide gene expression in response to endogenous cytokinin. Using MPSS (massively parallel signature sequencing) we identified 823 and 917 genes that were up- and downregulated, respectively, following 24 h of IPT induction. When comparing the response to cytokinin after 6 and 24 h, we identified different clusters of genes showing a similar course of regulation. Our study provides researchers with the opportunity to rapidly assess whether genes of interest are regulated by cytokinins.
authors: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. shoth@biologie.uni-erlangen.de
authors: Stefan
authors: S
authors: Hoth
authors: Yoshihisa
authors: Y
authors: Ikeda
authors: Michele
authors: M
authors: Morgante
authors: Xiujie
authors: X
authors: Wang
authors: Jianru
authors: J
authors: Zuo
authors: Michael K
authors: MK
authors: Hanafey
authors: Terry
authors: T
authors: Gaasterland
authors: Scott V
authors: SV
authors: Tingey
authors: Nam-Hai
authors: NH
authors: Chua
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005810:Multigene Family / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000302:isolation & purification; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D018749:RNA, Plant; D014157:Transcription Factors; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase