2,3,7,8-tetrachlorodibenzo-p-dioxin selectively enhances spontaneous IgE production in B cells from atopic patients.
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spontaneous IgE production was studied in B cells from atopic patients with allergic rhinitis, atopic eczema/dermatitis syndrome or bronchial asthma, and from non-atopic subjects. TCDD enhanced spontaneous IgE production in B cells from atopic patients without affecting production of IgG1, IgG2, IgG3, IgG4, IgM, IgA1 and IgA2, while TCDD failed to induce IgE production in B cells from non-atopic subjects. Purified surface IgE+ (sIgE+) B cells from atopic patients spontaneously produced IgE, while surface IgE- (sIgE-) B cells failed to do so. TCDD enhanced spontaneous IgE production in sIgE+ B cells, while TCDD with or without IL-4 or anti-CD40 mAb failed to induce IgE production in sIgE-B cells. Collectively, TCDD selectively enhanced ongoing IgE production. These results suggest that TCDD may aggravate allergic diseases by enhancing IgE-mediated allergic responses.
Kimata
Hajime
H
Department of Allergy, Ujitakeda Hospital, 24-1, Umonji, Uji, Uji-City, Kyoto Prefecture, 611-0021, Japan. unitikah@m12.alphanet.ne.jp
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000276:immunology*; D004785:Environmental Pollutants / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000187:drug effects*; D008297:Male; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*
D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D007073:Immunoglobulin E