title: [Experimental research on human spermatozoa membrane proteins by two-dimensional gel electrophoresis].
abstract: OBJECTIVE
  To analyze human spermatozoa membrane proteins by two-dimensional gel electrophoresis and to provide a basis for drawing the protein map of normal human spermatozoa membrane proteins.
  
  
  METHODS
  Spermatozoa were purified by Percoll density centrifugation, and spermatozoa membrane proteins were analyzed by two-dimensional gel electrophoresis using isoelectric focusing and polyacrylamide gel electrophoresis.
  
  
  RESULTS
  About 800 protein spots could be identified by the imaging analysis system. The molecular weight and isoelectric point of most proteins were 20,000 to 100,000 and 3.0 to 7.0 respectively.
  
  
  CONCLUSIONS
  There are more than 800 types of proteins in the human spermatozoa membrane. The spermatozoa membrane protein can be identified with a certain precision by two-dimensional gel electrophoresis.
authors: Department of Urology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. wangblack@citiz.net
authors: Hao-Fei
authors: HF
authors: Wang
authors: Zu-Qiong
authors: ZQ
authors: Xiang
authors: Yi-Xin
authors: YX
authors: Wang
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry*; D008970:Molecular Weight; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins