title: Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR.
abstract: We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of bacterial isolates via computer software.
authors: Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors: Kiersten J
authors: KJ
authors: Meacham
authors: Lixin
authors: L
authors: Zhang
authors: Betsy
authors: B
authors: Foxman
authors: Richard J
authors: RJ
authors: Bauer
authors: Carl F
authors: CF
authors: Marrs
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005060:Europe; D005843:Geography; D006801:Humans; D007438:Introns; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers