title: Kinetics of DNA binding with chloroquine phosphate using capacitive sensing method.
abstract: The capacitive sensing method has been applied to study the binding of DNA with chloroquine phosphate. DNA was immobilized on a gold electrode surface, self-assembled with thioglycolic acid. The results of a quartz crystal impedance (QCI) study indicate that the reaction of double-strand DNA (dsDNA) with chloroquine includes a fast electrostatic attraction and a slow intercalation of chloroquine into double-strand helix. The real-time experimental data obtained by capacitive sensing also revealed two distinctive kinetics stages during binding of dsDNA with chloroquine, while only one stage exists during reaction of single-strand DNA (ssDNA) with chloroquine. The kinetic parameters were obtained by fitting the real-time experimental data using a two stage reaction model. The rate constants of electrostatic attraction for dsDNA and ssDNA are estimated as 0.014 and 0.018 s(-1), respectively. The rate constant of the second stage of dsDNA is 0.0011 s(-1).
authors: State Key Laboratory of Chemo/Biological Sensing and Chemometrics, Hunan University, Changsha 410082, China.
authors: Fan
authors: F
authors: Yin
authors: Manli
authors: M
authors: Guo
authors: Shouzhuo
authors: S
authors: Yao
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002738:Chloroquine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D007700:Kinetics; D046911:Macromolecular Substances; D009862:Online Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D046911:Macromolecular Substances; C023676:chloroquine diphosphate; D002738:Chloroquine; D004247:DNA