Catalase-peroxidase activity has no influence on virulence in a murine model of tuberculosis.
The capacity to generate a chronic and persistent infection in the experimental murine model of tuberculosis induced aerogenically by a low-dose inoculum was determined in eight isoniazid-resistant clinical strains of Mycobacterium tuberculosis showing different catalase-peroxidase (C-P) activities. Determination of bacillary concentration in lung and spleen and the percentage of pulmonary parenchyma occupied by granulomas were monitored. Data showed no relation between the lack of C-P activity and the ability to develop a persistent infection, highlighting the potential of C-P negative strains to spread through the community.
Cardona
Pere Joan
PJ
Unitat de Tuberculosi Experimental, Department of Microbiology, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Crta del Canyet s/n, Badalona, 08916 Catalonia, Spain. pcardona@ns.hugtip.scs.es
Gordillo
Sergi
S
Amat
Isabel
I
Díaz
Jorge
J
Lonca
Joan
J
Vilaplana
Cristina
C
Pallarés
Angeles
A
Llatjós
Roger
R
Ariza
Aurelio
A
Ausina
Vicenç
V
D000818:Animals; D000995:Antitubercular Agents / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D007538:Isoniazid / Q000627:therapeutic use; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology* / Q000472:pathogenicity*; D010544:Peroxidases / Q000378:metabolism*; D010641:Phenotype; D013053:Spectrophotometry / Q000379:methods; D014376:Tuberculosis / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology; D018088:Tuberculosis, Multidrug-Resistant / Q000201:enzymology / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D014774:Virulence
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria; D007538:Isoniazid