Clinical relevance of soluble HLA and interaction of papain derived class I molecules with alloreactive CTL.
MHC class I and class II molecules are expressed in soluble form in the serum of both healthy and diseased individuals. Our aim was to investigate whether soluble class I (sHLA) levels in allograft patients correlate with their clinical status. Altogether, 20 renal and 30 cardiac graft recipients were examined. High levels of sHLA were measured at least 5 days preceding acute rejection episodes. Immune complexes between anti-HLA antibodies and sHLA were detected in a patient who died of a severe vascular rejection. In another study the interaction of papain-derived sHLA on alloreactive CTL in vitro was investigated. In a chromium-51-release cytotoxicity assay, 1.25 microg/ml of papain-digested class I molecules reduced CTL cytotoxicity to background levels. On the contrary, immobilized molecules triggered the release of serine esterase allospecifically. These data showed that the MHC molecule alone was a sufficient ligand for the interaction with alloreactive CTL.
Zavazava
N
N
Institute of Immunology, University of Kiel, Kiel, Germany.
Hausmann
R
R
Kraatz
E
E
Müller-Ruchholtz
W
W
D015415:Biomarkers / Q000097:blood; D004950:Esterases / Q000097:blood; D006680:HLA Antigens / Q000097:blood*; D016027:Heart Transplantation / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000097:blood / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D010206:Papain; D012995:Solubility; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology; D016896:Treatment Outcome
D015415:Biomarkers; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D004950:Esterases; C022635:serine esterase; D010206:Papain