title: 3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected types of processed and prepared foods: collaborative study.
abstract: The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Five foods--frozen lasagna, custard, frozen mixed vegetables, frozen hashbrowns, and frozen batter-coated mushrooms--were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 5 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.
authors: rtech laboratories, PO Box 64101, St. Paul, MN 55164-0101, USA. kmsilbernagel@landolakes.com
authors: Karen M
authors: KM
authors: Silbernagel
authors: Robert P
authors: RP
authors: Jechorek
authors: Charles N
authors: CN
authors: Carver
authors: Barbara L
authors: BL
authors: Horter
authors: Kathryn G
authors: KG
authors: Lindberg
mesh_terms: D000363:Agaricales; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D005511:Food Handling; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D005615:Freezing; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014675:Vegetables / Q000382:microbiology