title: Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine.
abstract: In order to obtain monoclonal antibodies that might have prophylactic applications and to understand better the immune response to hepatitis E virus (HEV), we used phage display to isolate chimpanzee antibodies to HEV. The panning antigen was an two open reading frame (ORF2) recombinant protein that elicits a broadly protective immune response in vaccinated monkeys. Two major antigenic sites were identified on the ORF2 protein: one site was not accessible on the surface of infectious virions but the other site was accessible to antibodies and was recognized specifically by antibodies that neutralize the virus.
authors: Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Darren J
authors: DJ
authors: Schofield
authors: Robert H
authors: RH
authors: Purcell
authors: Hanh T
authors: HT
authors: Nguyen
authors: Suzanne U
authors: SU
authors: Emerson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000956:Antigens, Viral / Q000187:drug effects*; D001710:Biotin; D015153:Blotting, Western; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D016752:Hepatitis E virus / Q000187:drug effects*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008253:Macaca mulatta; D002679:Pan troglodytes; D019151:Peptide Library; D011946:Receptors, Antigen / Q000187:drug effects*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D011946:Receptors, Antigen; D014765:Viral Vaccines; D001710:Biotin