Study on the interaction between nucleic acids and acetamiprid.
The interaction between deoxyribonucleic acid (DNA) and acetamiprid was studied. It was found that the fluorescence of acetamiprid could be enhanced in the presence of DNA in sulfuric acid solution. The excitation and emission wavelength of acetamiprid was 291 nm and 587 nm, respectively. Under optimal conditions, the calibration graph is over the range of 0.1-10 micromL(-1). The calibration limit is 0.06 microg mL(-1) (S/N = 3). The determination results of DNA in yeast cell and golden staphylococcus samples by this method were satisfactory. The mechanism of the reaction is discussed.
Jie
Nianqin
N
College of Applied Chemistry, China Agricultural University, Beijing, PR China. aydy-826@163.com
Hou
Shicong
S
Du
Fengpei
F
Huang
Linrui
L
Jiang
Guibin
G
Lv
Suqin
S
D002138:Calibration; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004271:DNA, Fungal / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D015394:Molecular Structure; D000073943:Neonicotinoids; D009691:Nucleic Acid Denaturation; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013210:Staphylococcus / Q000235:genetics; D013464:Sulfuric Acids; D013997:Time Factors; D015003:Yeasts / Q000235:genetics
D004269:DNA, Bacterial; D004271:DNA, Fungal; D000073943:Neonicotinoids; D011725:Pyridines; D013464:Sulfuric Acids; C464485:acetamiprid; D004247:DNA; C033158:sulfuric acid