title: Correlation of biochemical and hematological changes with graft failure following pig heart and kidney transplantation in baboons.
abstract: We have explored biochemical and hematologic parameters that might indicate acute humoral xenograft rejection (AHXR) following pig organ transplantation in baboons. Baboons (n = 15) received an immunosuppressive regimen, and underwent a miniature swine or hDAF kidney (Group 1, n = 6) or heart (Group 2, n = 7) transplantation. Control baboons (Group 3, n = 2) received the immunosuppressive regimen without organ transplantation. Blood chemistry and hematologic parameters were measured daily. Baboon and porcine cytomegalovirus were monitored. In Groups 1 and 2, organ grafts survived for up to 29 days. A plasma fibrinogen of <80 mg/dL on 2 consecutive days, and a serum lactate dehydrogenase of >600 U/L and aspartate transaminase of >300 U/L, were associated with the development of AHXR in both heart and kidney grafts. In Group 1, a decrease in platelet count of >150,000/microL within 3 days, or a count of <50,000/microL, were associated with AHXR. In Group 2, a creatine phosphokinase of >500 U/L was associated with graft failure. In Group 3, no abnormalities were observed. The possibility that porcine CMV may play a role in graft injury could not be excluded. Noninvasive parameters were identified that have predictive potential for AHXR. Monitoring of these might enable therapeutic intervention to reverse rejection.
authors: Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.
authors: Christoph
authors: C
authors: Knosalla
authors: Bernd
authors: B
authors: Gollackner
authors: Leo
authors: L
authors: Bühler
authors: Nicolas J
authors: NJ
authors: Mueller
authors: Stuart
authors: S
authors: Houser
authors: Shamila
authors: S
authors: Mauiyyedi
authors: David H
authors: DH
authors: Sachs
authors: Simon C
authors: SC
authors: Robson
authors: Jay
authors: J
authors: Fishman
authors: Henk-Jan
authors: HJ
authors: Schuurman
authors: Michel
authors: M
authors: Awwad
authors: David K C
authors: DK
authors: Cooper
mesh_terms: D000818:Animals; D004211:Disseminated Intravascular Coagulation / Q000276:immunology; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010215:Papio / Q000276:immunology; D013552:Swine / Q000276:immunology; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000276:immunology*
chemical_list: D006454:Hemoglobins; D007770:L-Lactate Dehydrogenase