Chromosomal assignment of 20 candidate genes for canine congenital sensorineural deafness by FISH and RH mapping.
The analysis of inherited diseases in the domestic dog (Canis familiaris) provides a resource for the continued use of this species as a model system for human diseases. Many different dog breeds are affected by congenital sensorineural deafness. Since mutations in various genes have already been found causative for sensorineural hearing impairment in humans or mice, 20 of these genes were considered as candidates for deafness in dogs. For each of the candidate genes a canine BAC clone was isolated by screening with heterologous human or murine cDNA probes. The gene-containing BAC clones were physically assigned to the canine genome by FISH and the BAC-derived STS-markers were positioned with the RHDF5000 panel on the canine RH map. The mapping data, which confirm the established conservation of synteny between canine and human chromosomes, provide a resource for further association studies in segregating canine populations and the basis for new insights into this common canine and human disease.
Rak
S G
SG
Institute of Animal Breeding and Genetics, School of Veterinary Medicine, Hannover, Germany. Simone.Rak@tiho-hannover.de
Drögemüller
C
C
Leeb
T
T
Quignon
P
P
André
C
C
Scott
A
A
Breen
M
M
Distl
O
O
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D003638:Deafness / Q000235:genetics / Q000662:veterinary*; D004285:Dogs; D020022:Genetic Predisposition to Disease; D006319:Hearing Loss, Sensorineural / Q000235:genetics / Q000662:veterinary*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D021101:Radiation Hybrid Mapping / Q000662:veterinary; D026801:Synteny