Batch growth of Salmonella typhimurium LT2: stoichiometry and factors leading to cessation of growth.
Salmonella typhimurium LT2 was grown in batch culture (trypticase soy broth, with 0.3%(w/v) yeast extract, 1% (w/v) glucose and 0.5% (w/v/) NaCl, 20 degrees C) at a range of initial pH (4.4, 4.8, 5.0 and 7.0). The consumption of oxygen and glucose was found to be independent of initial pH, and stoichiometric with growth. Mean yield coefficients of 6.9 x 10(-15) and 15.5 x 10(-15) mol oxygen/cell were estimated. Calculation of the instantaneous state of carbon during the cultivation showed stoichiometric conversion of glucose into biomass, carbon dioxide and organic acids. The concentration of the undissociated form of the primary acidic product (acetic acid) was shown to be the factor limiting growth.
Wilson
P D G
PD
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
Wilson
D R
DR
Brocklehurst
T F
TF
Coleman
H P
HP
Mitchell
G
G
Waspe
C R
CR
Jukes
S A
SA
Robins
M M
MM
D019342:Acetic Acid / Q000378:metabolism / Q000494:pharmacology*; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D002245:Carbon Dioxide; D019342:Acetic Acid; D010100:Oxygen