title: Cloning of the zebra finch androgen synthetic enzyme CYP17: a study of its neural expression throughout posthatch development.
abstract: Male zebra finches develop a robust neural song system that supports singing, but females have a minimal song circuit and do not sing. Estrogens masculinize the song circuit and are especially potent during the first 3 weeks of posthatch development. The gonads do not seem to supply the masculinizing steroids, implying that another tissue synthesizes steroids. Evidence suggests that the brain is capable of synthesizing neurosteroids, which in developing zebra finches may be required for song system differentiation. Aromatase, the enzyme that synthesizes estrogen from androgen, is equally abundant in male and female brains. To investigate further the potential for neurosteroidogenesis in the zebra finch brain, we cloned and examined the expression of 17alpha-hydroxylase/17,20 lyase (CYP17), the enzyme that synthesizes the androgenic substrate for aromatase. We used Northern blots, reverse transcription-polymerase chain reaction, and in situ hybridization to show that CYP17 is transcribed in developing and adult brains. CYP17 is transcribed at developmental stages and in brain areas potentially important to aspects of the developing song system, although no sex difference was detected in mRNA levels. Our results support the hypothesis that neurosteroids may act to influence brain organization and function in the zebra finch.
authors: Interdepartmental Graduate Program in Neuroscience, University of California--Los Angeles, Los Angeles, California 90095, USA. slondon@ucla.edu
authors: Sarah E
authors: SE
authors: London
authors: Jim
authors: J
authors: Boulter
authors: Barney A
authors: BA
authors: Schlinger
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D012737:Sex Factors; D020308:Songbirds; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D013254:Steroid 17-alpha-Hydroxylase