title: The Hansenula polymorpha (strain CBS4732) genome sequencing and analysis.
abstract: The methylotrophic yeast Hansenula polymorpha is a recognised model system for investigation of peroxisomal function, special metabolic pathways like methanol metabolism, of nitrate assimilation or thermostability. Strain RB11, an odc1 derivative of the particular H. polymorpha isolate CBS4732 (synonymous to ATCC34438, NRRL-Y-5445, CCY38-22-2) has been developed as a platform for heterologous gene expression. The scientific and industrial significance of this organism is now being met by the characterisation of its entire genome. The H. polymorpha RB11 genome consists of approximately 9.5 Mb and is organised as six chromosomes ranging in size from 0.9 to 2.2 Mb. Over 90% of the genome was sequenced with concomitant high accuracy and assembled into 48 contigs organised on eight scaffolds (supercontigs). After manual annotation 4767 out of 5933 open reading frames (ORFs) with significant homologies to a non-redundant protein database were predicted. The remaining 1166 ORFs showed no significant similarity to known proteins. The number of ORFs is comparable to that of other sequenced budding yeasts of similar genome size.
authors: Institute for Microbiology, Heinrich-Heine University Düsseldorf, Universitätsstrasse 1, 40225, Düsseldorf, Germany. ramezani@uni-duesseldorf.de
authors: Massoud
authors: M
authors: Ramezani-Rad
authors: Cornelis P
authors: CP
authors: Hollenberg
authors: Juergen
authors: J
authors: Lauber
authors: Holger
authors: H
authors: Wedler
authors: Eike
authors: E
authors: Griess
authors: Christian
authors: C
authors: Wagner
authors: Kaj
authors: K
authors: Albermann
authors: Jean
authors: J
authors: Hani
authors: Michael
authors: M
authors: Piontek
authors: Ulrike
authors: U
authors: Dahlems
authors: Gerd
authors: G
authors: Gellissen
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D030562:Databases, Protein; D015723:Gene Library; D005800:Genes, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010843:Pichia / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal