[Viability of heterotrophic bacteria in the Bay of Marseilles].
Marine microorganism activities are commonly assessed by bulk methods and assigned to the total cell count. The presence in significant amounts of ghost, dead, and damaged cells makes such as assignment a non-correct one. A Nucleic Acid Double Staining protocol (NADS) of fresh water bacteria (Barbesti et al., Cytometry 40 (2000) 214-218) has been adapted to resolve viable, damaged and dead cells in marine environments (Grégori et al., Appl. Environ. Microbiol. 67 (2001) 4662-4670). The present reports the first in situ application of this approach, conducted in the Bay of Marseilles in winter and spring periods at two sites with contrasted features.
Grégori
Gérald
G
Purdue University Cytometry Laboratories, Hansen Life Sciences Research Building, Room B050, 201 S. University Street, West Lafayette, IN 47907-2064, USA.
Denis
Michel
M
Lefèvre
Dominique
D
Romano
Jean-Claude
JC
D001419:Bacteria / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D002463:Cell Membrane Permeability; D004269:DNA, Bacterial / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer; D008386:Marine Biology / Q000379:methods; D008522:Mediterranean Sea; D012621:Seasons; D013194:Staining and Labeling / Q000379:methods
D004269:DNA, Bacterial