title: Microfluidic systems in proteomics.
abstract: We present the state-of-the-art in miniaturized sample preparation, immunoassays, one-dimensional and multidimensional analyte separations, and coupling of microdevices with electrospray ionization-mass spectrometry. Hyphenation of these different techniques and their relevance to proteomics will be discussed. In particular, we will show that analytical performances of microfluidic analytical systems are already close to fulfill the requirements for proteomics, and that miniaturization results at the same time in a dramatic increase in analysis throughput. Throughout this review, some examples of analytical operations that cannot be achieved without microfluidics will be emphasized. Finally, conditions for the spreading of microanalytical systems in routine proteomic labs will be discussed.
authors: Laboratoire d'Electrochimie Physique et Analytique (LEPA), Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors: Niels
authors: N
authors: Lion
authors: Tatiana C
authors: TC
authors: Rohner
authors: Loïc
authors: L
authors: Dayon
authors: Isabelle L
authors: IL
authors: Arnaud
authors: Eugen
authors: E
authors: Damoc
authors: Nikolay
authors: N
authors: Youhnovski
authors: Zhi-Yong
authors: ZY
authors: Wu
authors: Christophe
authors: C
authors: Roussel
authors: Jacques
authors: J
authors: Josserand
authors: Henrik
authors: H
authors: Jensen
authors: Joël S
authors: JS
authors: Rossier
authors: Michael
authors: M
authors: Przybylski
authors: Hubert H
authors: HH
authors: Girault
mesh_terms: D004586:Electrophoresis / Q000379:methods; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D044085:Microfluidics / Q000379:methods*; D008904:Miniaturization / Q000379:methods; D020543:Proteome
chemical_list: D004798:Enzymes; D020543:Proteome