Analysis of serotype AD strains from F1 progenies between urease-positive- and negative-strains of Cryptococcus neoformans.
Cryptococcus neoformans is a pathogenic basidiomycete with a defined sexual cycle involving mating between haploid yeast cells with a transient diploid state. We examined F1 progeny from a crossing between the urease-negative strain (environmental isolate, serotype A, mating type alfa, haploid) and a tester strain (B 3502 from NIH of USA; urease-positive, serotype D, mating type a, haploid) for serotype, mating type, ploidy and urease activity, and performed partial sequencing of the urease gene. Phenotypes of the F1 progeny and results of SSCP analyses suggested that the serotype AD strain of the F1 progeny is a hybrid of the parental serotype A and D strains.
Tanaka
Reiko
R
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Nishimura
Kazuko
K
Imanishi
Yumi
Y
Takahashi
Ichiro
I
Hata
Yoko
Y
Miyaji
Makoto
M
D003455:Cryptococcus neoformans / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D012703:Serotyping; D014510:Urease / Q000032:analysis
D014510:Urease