Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate.
A high-performance liquid chromatography with electrochemical detection (HPLC-ED) method is described for determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) in microdialysate from the skeletal muscle interstitial space. Using a microdialysis technique, we sampled 30 microl dialysate from the skeletal muscle interstitial space and injected dialysate directly into HPLC-ED system. The control MHPG concentration of dialysate was 213+/-18 pg/ml. The MHPG concentrations were reduced by entacapone (catechol-O-methyltransferase inhibitor, COMT), augmented by local infusion of dihydroxyphenylglycol. This system offers a new possibility for simple, rapid monitoring of MHPG as an index of COMT activity in skeletal muscle.
Tokunaga
Noriyuki
N
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Fujishiro-dai 5-7-1, Suita, Osaka 565-8565, Japan.
Yamazaki
Toji
T
Akiyama
Tsuyoshi
T
Mori
Hidezo
H
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis*; D017551:Microdialysis; D018482:Muscle, Skeletal / Q000737:chemistry*; D011817:Rabbits
D008734:Methoxyhydroxyphenylglycol