Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience.
Based on time-averaging fluctuation analysis of small fluorescent molecular ensembles in equilibrium, fluorescence correlation spectroscopy has recently been applied to investigate processes in the intracellular milieu. The exquisite sensitivity of fluorescence correlation spectroscopy provides access to a multitude of measurement parameters (rates of diffusion, local concentration, states of aggregation and molecular interactions) in real time with fast temporal and high spatial resolution. The introduction of dual-color cross-correlation, imaging, two-photon excitation, and coincidence analysis coupled with fluorescence correlation spectroscopy has expanded the utility of the technique to encompass a wide range of promising applications in living cells that may provide unprecedented insight into understanding the molecular mechanisms of intracellular neurobiological processes.
Kim
Sally A
SA
Department of Neurobiology and Anatomy, P.O. Box 20708, University of Texas Health Science Center at Houston, Houston, TX 77225, USA. sally.kim@uth.tmc.edu
Schwille
Petra
P
D000818:Animals; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry*; D009488:Neurosciences / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods