title: Characteristics and mechanisms of the two types of photoelectric differential response of bacteriorhodopsin-based photocell.
abstract: Bacteriorhodopsin (BR)-based photocells have been assigned possessing differential photoelectric response. But recently we found that the differential response described before, which occurred in milliseconds to seconds, outputting a positive pulse when light was on and a negative pulse when light was off, was not the intrinsic property of the BR molecule. It was partially caused by the measuring circuit. By measuring the photoelectric response signal of the BR film photocell to a short laser pulse, the impulse response function of the BR film photocell was obtained by data fitting with MATLAB software. A simulation system was accordingly developed. The output response signals of the BR film photocell under different stepping incident light were calculated. By simulation and analysis, it was concluded that the differential response caused by the intrinsic property of the BR molecule happened in microseconds time scale, and it produced a negative pulse when light was on and a positive pulse when light was off. It was much faster but much weaker than that described before.
authors: State Key Laboratory of Transient Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, PO Box 80(25), Xi'an710068, P.R. China. yaobl@opt.ac.cn
authors: Baoli
authors: B
authors: Yao
authors: Yingli
authors: Y
authors: Wang
authors: Ming
authors: M
authors: Lei
authors: Yuan
authors: Y
authors: Zheng
mesh_terms: D001436:Bacteriorhodopsins / Q000378:metabolism* / Q000528:radiation effects*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004056:Differential Threshold; D004307:Dose-Response Relationship, Radiation; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D019544:Equipment Failure Analysis; D008027:Light; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods*; D018717:Purple Membrane / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001436:Bacteriorhodopsins