Physiological differentiation of DH-PBAN-producing neurosecretory cells in the silkworm embryo.
Embryonic diapause of the silkworm, Bombyx mori, is induced by a neuropeptide hormone, the diapause hormone (DH), which is secreted from a limited number of neurosecretory cells in the subesophageal ganglion (SG) at the maternal generation. We examined the developmental fate of the hormone-producing cell (DH-pheromone biosynthesis activating neuropeptide [PBAN]-producing cell) in the embryonic stage at the level of gene expression and cell biology. The DH-PBAN gene expression started at the histogenesis stage and gradually increased toward hatching. DH is an amidated peptide belonging to FXPRLamide family. The immunoreactive somata against anti FXPRLamide antiserum were found in the SG from blastokinesis. Immunoreactive neural processes with varicosites were also found on the corpus cardiacum and the corpus allatum. The implantation of a part of a developing embryo including the SG into the pupae with the SG removed induced diapause eggs in the progeny. These results were obtained from eggs incubated under diapause-averting conditions as well as diapause-inducing conditions. Thus, a neurosecretory system responsible for biosynthesis of FXPRLamide neuropeptides is established as early as histogenesis, although the system to regulate the secretion of neuropeptide hormones has not been fully formed by that time.
Morita
Akihiro
A
Laboratory of Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Chikusa Nagoya, Japan. amorita@med.osaka-u.ac.jp
Niimi
Teruyuki
T
Yamashita
Okitsugu
O
D000818:Animals; D015152:Blotting, Northern; D012831:Bombyx / Q000166:cytology / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics; D009479:Neuropeptides / Q000096:biosynthesis* / Q000235:genetics; D009490:Neurosecretory Systems / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D010063:Ovum / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013696:Temperature
D007301:Insect Hormones; D009479:Neuropeptides; D012333:RNA, Messenger; C075027:diapause hormone