[Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension].
To construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.



The SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP  -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI-  anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.



A chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.



Gene splicing by overlap extension is a successful specific PCR technique for gene recombination.
Yi
Ping-yong
PY
College of Medicine, Zhejiang University, Hangzhou 310006, China.
Yu
Hai
H
Ma
Wen-xue
WX
Wu
Wen-jun
WJ
Jiang
Huai
H
D000469:Alkaline Phosphatase; D001483:Base Sequence; D004768:Enterotoxins / Q000235:genetics*; D058851:GPI-Linked Proteins; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000235:genetics*
D004768:Enterotoxins; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; C026066:enterotoxin A, Staphylococcal; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental