Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning 1H nuclear magnetic resonance spectroscopy of liver tissues.
Principal component analysis (PCA) has been applied to three nuclear magnetic resonance (NMR) spectral editing methods, namely, the Carr-Purcell-Meiboom-Gill spin-echo, diffusion editing, and skyline projection of a two-dimensional J-resolved spectrum, obtained from high-resolution magic-angle spinning NMR spectroscopy of liver tissues, to distinguish between control and hydrazine-treated rats. The effects of the toxin on rat liver biochemistry were directly observed and characterized by depleted levels of liver glycogen, choline, taurine, trimethylamine N-oxide, and glucose and by elevated levels of lipids and alanine. The highly unsaturated omega-3-type fatty acid was observed for the first time in hydrazine-treated rat liver. The contributions of the metabolites to the separation of control from dosed liver tissues varied depending on the type of spectral editing method used. We have shown that subtle changes in the metabolic profiles can be selectively amplified using a metabonomics approach based on the different NMR spectral editing techniques in conjunction with PCA.
Wang
Yulan
Y
Biomedical Sciences Division, Faculty of Medicine, Imperial College, Sir Alexander Fleming Building, South Kensington, SW7 2AZ London, UK. yulan.wang@imperial.ac.uk
Bollard
Mary E
ME
Keun
Hector
H
Antti
Henrik
H
Beckonert
Olaf
O
Ebbels
Timothy M
TM
Lindon
John C
JC
Holmes
Elaine
E
Tang
Huiru
H
Nicholson
Jeremy K
JK
D000409:Alanine / Q000032:analysis; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D002794:Choline / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006834:Hydrazines / Q000008:administration & dosage / Q000494:pharmacology; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008744:Methylamines / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000032:analysis; D014118:Toxins, Biological / Q000008:administration & dosage
D006834:Hydrazines; D008055:Lipids; D008744:Methylamines; D014118:Toxins, Biological; D013654:Taurine; C029424:hydrazine; D006003:Glycogen; C005855:trimethyloxamine; D005947:Glucose; D002794:Choline; D000409:Alanine