title: Prospective study of hepatitis C virus infection in hemodialysis patients by monthly analysis of HCV RNA and antibodies.
abstract: The aims of this study were to (i) evaluate the prevalence and the incidence of hepatitis C virus (HCV) infection in hemodialysis patients in two different centers in São Paulo (Brazil), (ii) determine the time required to detect HCV infection among these patients by serology or PCR, (iii) establish the importance of alanine aminotransferase determination as a marker of HCV infection, and (iv) identify the HCV genotypes in this population. Serum samples were collected monthly for 1 year from 281 patients admitted to hospital for hemodialysis. Out of 281 patients, 41 patients (14.6%) were HCV positive; six patients seroconverted during this study (incidence = 3.1/1000 person-month). In 1.8% (5/281) of cases, RNA was detected before the appearance of antibodies (up to 5 months), and in 1.1% (3/281) of cases, RNA was the unique marker of HCV infection. The genotypes found were 1a, 1b, 3a, and 4a. The presence of genotype 4a is noteworthy, since it is a rare genotype in Brazil. These data pointed out the high prevalence and incidence of HCV infection at hemodialysis centers in Brazil and showed that routine PCR is fundamental for improving the detection of HCV carriers among patients undergoing hemodialysis.
authors: Laboratório de Hepatites do Serviço de Virologia do Instituto Adolfo Lutz, São Paulo, Brazil. rmoreira@ial.sp.gov.br
authors: Regina
authors: R
authors: Moreira
authors: João Renato Rebello
authors: JR
authors: Pinho
authors: Jorge
authors: J
authors: Fares
authors: Isabel Takano
authors: IT
authors: Oba
authors: Maria Regina
authors: MR
authors: Cardoso
authors: Cláudia Patara
authors: CP
authors: Saraceni
authors: Celso
authors: C
authors: Granato
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D015994:Incidence; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood*; D006435:Renal Dialysis / Q000009:adverse effects*; D017422:Sequence Analysis, DNA
chemical_list: D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D000410:Alanine Transaminase