Chromosomal localization of six repeated DNA sequences among species of Microtus (Rodentia).
C-banding and fluorescence in situ hybridization (FISH) document the distribution of constitutive heterochromatin and six highly repeated DNA families (MSAT2570, MSAT21, MSAT160, MS2, MS4 and STR47) in the chromosomes of nine species of Microtus (M. chrotorrhinus, M. rossiaemeridionalis, M. arvalis, M. ilaeus, M. transcaspicus, M. cabrerae, M. pennsylvanicus, M. miurus and M. ochrogaster). Autosomal heterochromatin is largely centromeric and contains different repeated families in different species. Similarly, large C-band positive blocks on the sex chromosomes of four species contain different repeated DNAs. This interspecific variation in the chromosomal distribution and copy number of the repeats suggests that a common ancestor to modern species contained most of the repetitive families, and that descendant species selectively amplified or deleted different repeats on different chromosomes.
Modi
William S
WS
Basic Research Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, MD 21702, USA. modi@ncifcrf.gov
Serdyukova
Natalya A
NA
Vorobieva
Nadezhda V
NV
Graphodatsky
Alexander S
AS
D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure*; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D006570:Heterochromatin / Q000235:genetics / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D006570:Heterochromatin; D004247:DNA