title: Human keratinocyte ATP2C1 localizes to the Golgi and controls Golgi Ca2+ stores.
abstract: Hailey-Hailey disease (MIM16960) is a blistering skin disease caused by mutations in the Ca2+ ATPase ATP2C1. We found that the abnormal Ca2+ signaling seen in Hailey-Hailey disease keratinocytes correlates with decreased protein levels of ATP2C1. Human ATP2C1 protein approximated 115 kDa in size. The ATP2C1 is localized to the Golgi apparatus in human keratinocytes, similar to its localization in yeast and Caenorhabditis elegans. To test whether the ATP2C1 controls Golgi Ca2+ stores, we measured intraorganelle Ca2+ concentrations using specifically targeted aequorins. Whereas normal keratinocytes display Golgi Ca2+ levels comparable to other epithelial cells, Hailey-Hailey disease keratinocyte Golgi Ca2+ refill is slower, and the maximum Ca2+ concentration reached is significantly lower. These findings were replicated in vivo, because clinically normal Hailey-Hailey disease epidermis contained lower Ca2+ stores and displayed an abnormal Ca2+ gradient. In this report we localize the ATP2C1, demonstrate its physiologic relevance in mammalian cells, and measure intraorganelle Golgi Ca2+ in keratinocytes.
authors: Department of Dermatology, University of California, San Francisco, California, USA. behnemj@itsa.ucsf.edu
authors: Martin J
authors: MJ
authors: Behne
authors: Chia-Ling
authors: CL
authors: Tu
authors: Ida
authors: I
authors: Aronchik
authors: Ervin
authors: E
authors: Epstein
authors: Graham
authors: G
authors: Bench
authors: Daniel D
authors: DD
authors: Bikle
authors: Tullio
authors: T
authors: Pozzan
authors: Theodora M
authors: TM
authors: Mauro
mesh_terms: D000328:Adult; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D016506:Pemphigus, Benign Familial / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
chemical_list: C403624:ATP2C1 protein, human; D000252:Calcium-Transporting ATPases; D002118:Calcium