Analysis of aristolochic acid in nine sources of Xixin, a traditional Chinese medicine, by liquid chromatography/atmospheric pressure chemical ionization/tandem mass spectrometry.
Aristolochic acid I (AA-I), which is a known nephrotoxin, is found in a commonly used Chinese medicine, Xixin, that originates from nine Asarum species (Aristolochiaceae) found in China. A method has been developed using reversed-phase liquid chromatography coupled with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry under the positive ion detection mode [LC/(+)APCI/MS/MS] to determine the amount of AA-I in Xixin. The limit of detection of AA-I, estimated by monitoring with LC/MS/MS, was at the low microg/l level. By applying this method to methanol extracts of nine Asarum species, the concentrations of AA-I were found to range from 3.3 ng/mg (Asarum sieboldii) to 3376.9 ng/mg (Asarum crispulatum).
Jong
Ting-Ting
TT
Department of Chemistry, National Chung-Hsin University, Taichung, Taiwan. ttjong@mail.nchu.edu.tw
Lee
Maw-Rong
MR
Hsiao
Shun-Sheng
SS
Hsai
Jar-Lung
JL
Wu
Tian-Shung
TS
Chiang
Shu Tuan
ST
Cai
Shao-Qing
SQ
D034341:Aristolochic Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D008516:Medicine, Chinese Traditional / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; C000228:aristolochic acid I