title: Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGC (primordial germ cell)-containing graft.
abstract: In germ-line chimera, gametes originate from both the donor and recipient. In order to increase the proportion of gametes from the donor, the elimination or reduction of primordial germ cells (PGCs) from the recipient is required. In the present study, histological and genetic analyses were performed in the chimeric fish obtained when sterile goldfish x common carp hybrid and fertile goldfish embryos were used as a recipient and donor, respectively. Chimerism was induced by transplantation of the lower part of the goldfish blastoderm into the hybrid blastoderm at the blastula stage. Neither spermatid nor spermatozoa were observed in the testis of the male hybrid. Motile sperm were obtained from 15 chimeric males by human chorionic gonadotropin (HCG) injection. When the sperm of chimeric fish were genetically analyzed, only goldfish-specific repetitive DNA sequences were detected. These results revealed that chimeric fish of the cross between a sterile male hybrid and fertile goldfish produced sperm exclusively derived from the donor goldfish.
authors: Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Kameda, Nanae 041-1105, Japan. eyemaha@fsc.hokudai.ac.jp
authors: Etsuro
authors: E
authors: Yamaha
authors: Masaru
authors: M
authors: Murakami
authors: Koji
authors: K
authors: Hada
authors: Satoshi
authors: S
authors: Otani
authors: Takafumi
authors: T
authors: Fujimoto
authors: Minoru
authors: M
authors: Tanaka
authors: Suzu
authors: S
authors: Sakao
authors: Shizuo
authors: S
authors: Kimura
authors: Shoh
authors: S
authors: Sato
authors: Katsutoshi
authors: K
authors: Arai
mesh_terms: D000818:Animals; D001756:Blastoderm / Q000637:transplantation; D002347:Carps; D017690:Cell Transplantation; D003433:Crosses, Genetic; D004625:Embryo, Nonmammalian / Q000166:cytology; D005260:Female; D005298:Fertility; D005306:Fertilization; D005854:Germ Cells / Q000166:cytology*; D006054:Goldfish; D006066:Gonads / Q000196:embryology; D006824:Hybridization, Genetic; D008297:Male; D018183:Transplantation Chimera