[Studies on fractionation and protective immunity of the membrane antigen from hepato-portal juveniles of Schistosoma japonicum].
OBJECTIVE
To explore the immunological characteristics of the membrane antigen from hepato-portal juveniles of Schistosoma japonicum and its protective immunity against S. japonicum (Sj) in mice.


METHODS
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and enzyme-linked immune electro-transfer blot(EITB) methods were used to recognize the membrane antigens from hepato-portal schistosomula (SjHmAg) by infected rabbit sera (IRS) and normal rabbit sera (NRS). Kunming mice were immunized subcutaneously three times(0, 2, 4 weeks) with SjHmAg. Each mouse was challenged with 40 +/- 1 cercariae. Six weeks later the mice were killed, worms and liver eggs were counted.


RESULTS
7 major protein bands appeared on SDS-PAGE. IRS mainly reacted specifically with SjHmAg of 23, 33 and 63 kDa. Compared with the control groups, the reduction rate of worms and eggs per gram liver in the experimental group were 16.2% and 54.4%, respectively.


CONCLUSIONS
Different protein components from SjHmAg are obtained using SDS-PAGE, and the antigen can induce a protective immunity against Sj in mice.
Wang
Min
M
Department of Clinical Laboratory, Second Xiangya Hospital of Central South University, Changsha 410011. wangmin0000@yahoo.com.cn
Yi
Xin-yuan
XY
Zeng
Xian-fang
XF
Zhou
Dong-ming
DM
Zhang
Shun-ke
SK
Zhang
Jie
J
Yuan
Shi-shan
SS
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology*; D015151:Immunoblotting; D008099:Liver / Q000469:parasitology; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D010270:Parasite Egg Count; D011168:Portal System / Q000469:parasitology; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*
D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins