Wolinella succinogenes quinol:fumarate reductase and its comparison to E. coli succinate:quinone reductase.
The three-dimensional structure of Wolinella succinogenes quinol:fumarate reductase (QFR), a dihaem-containing member of the superfamily of succinate:quinone oxidoreductases (SQOR), has been determined at 2.2 A resolution by X-ray crystallography [Lancaster et al., Nature 402 (1999) 377-385]. The structure and mechanism of W. succinogenes QFR and their relevance to the SQOR superfamily have recently been reviewed [Lancaster, Adv. Protein Chem. 63 (2003) 131-149]. Here, a comparison is presented of W. succinogenes QFR to the recently determined structure of the mono-haem containing succinate:quinone reductase from Escherichia coli [Yankovskaya et al., Science 299 (2003) 700-704]. In spite of differences in polypeptide and haem composition, the overall topology of the membrane anchors and their relative orientation to the conserved hydrophilic subunits is strikingly similar. A major difference is the lack of any evidence for a 'proximal' quinone site, close to the hydrophilic subunits, in W. succinogenes QFR.
Lancaster
C Roy D
CR
Max-Planck-Institut für Biophysik, Abteilung Molekulare Membranbiologie, Marie-Curie-Str. 15, D-60439 Frankfurt am Main, Germany. roy.lancaster@mpibp-frankfurt.mpg.de
D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004579:Electron Transport; D042963:Electron Transport Complex II / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D019143:Evolution, Molecular; D006418:Heme / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits; D018892:Proton-Motive Force; D017386:Sequence Homology, Amino Acid; D016968:Wolinella / Q000201:enzymology* / Q000235:genetics
D021122:Protein Subunits; D006418:Heme; D010088:Oxidoreductases; D042963:Electron Transport Complex II; C424907:quinol fumarate reductase