title: The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway.
abstract: The Arabidopsis thalianachlorophyll a/b binding protein underexpressed (cue1) mutant, which has been isolated in a screen for chlorophyll a/b binding protein (CAB) underexpressors, exhibits a reticulate leaf phenotype combined with delayed chloroplast development and aberrant shape of the palisade parenchyma cells. The affected gene in cue1 is a phosphoenolpyruvate (PEP)/phosphate translocator (PPT) of the plastid inner envelope membrane. The proposed function of the PPT in C3-plants is the import of PEP into the stroma as one of the substrates for the shikimate pathway, from which aromatic amino acids and a variety of secondary plant products derive. The mutant phenotype could be: (i) complemented by constitutive overexpression of a heterologous PPT from cauliflower; and (ii) rescued by overexpression of a C4-type pyruvate,orthophosphate dikinase (PPDK). The latter approach indicates that PEP deficiency within plastids triggers developmental constraints in cue1. The impact of the mutation on aspects of primary and secondary metabolism was assessed in cue1 as well as in the individual transformant lines. The majority of the data obtained in this and an accompanying paper suggest that the mutant phenotype is not simply caused by a general restriction of the shikimate pathway because of a defect in a PPT.
authors: Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, D-50931 Köln, Germany.
authors: Lars
authors: L
authors: Voll
authors: Rainer E
authors: RE
authors: Häusler
authors: Rolf
authors: R
authors: Hecker
authors: Andreas
authors: A
authors: Weber
authors: Gottfried
authors: G
authors: Weissenböck
authors: Gabriele
authors: G
authors: Fiene
authors: Sabine
authors: S
authors: Waffenschmidt
authors: Ulf-Ingo
authors: UI
authors: Flügge
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D045342:Light-Harvesting Protein Complexes / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D010641:Phenotype; D010788:Photosynthesis; D015183:Restriction Mapping; D012765:Shikimic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; C491608:CUE1 protein, Arabidopsis; D002352:Carrier Proteins; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D012765:Shikimic Acid