Does glutathione S-transferase Pi (GST-Pi) a marker protein for cancer?
Glutathione S-transferases (GSTs, EC 2.5.1.18) are multifunctional and multigene products. They are versatile enzymes and participate in the nucleophilic attack of the sulphur atom of glutathione on the electrophilic centers of various endogenous and xenobiotic compounds. Out of the five, alpha, micro, pi, sigma and theta, major classes of GSTs, GST-pi has significance in the diagnosis of cancers as it is expressed abundantly in tumor cells. This protein is a single gene product, coded by seven exons, that is having 24 kDa mass and pI value of 7.0. Four upstream elements such as two enhancers, and one of each of AP-1 site and GC box regulate pi gene. During chemical carcinogenesis because of jun/fos oncogenes (AP-1) regulatory elements, specifically GST-pi is expressed in liver. Therefore this gene product could be used as marker protein for the detection of chemical toxicity and carcinogenesis.
Aliya
S
S
Department of Biochemistry, S. V. University, Tirupathi, India.
Reddanna
P
P
Thyagaraju
K
K
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004789:Enzyme Activation; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D051381:Rats; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D014408:Biomarkers, Tumor; D007527:Isoenzymes; D018808:Transcription Factor AP-1; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C496558:Gstp1 protein, rat