Coupling of a large-size capillary column with an electrospray mass spectrometer. A reliable and sensitive sheath flow capillary electrophoresis-mass spectrometry interface.
The concept of interfacing a large-size column for capillary electrophoresis (CE) to electrospray ionization mass spectrometry (ESI-MS) for robust and automatic CE-MS operation is reported. Both standard ionspray interface and microionspray interface have been modified to operate in a sheath flow pattern to overcome the common stability problem in CE-MS coupling. To make the interface sensitive, a step-down stainless steel tube with smaller inner diameter and tapered tip was incorporated onto a larger tube embracing the CE column via cold soldering. The devices were evaluated for quantitative analysis of nucleotides at femtomole level and stable analytical performance in peptide profiling.
Liu
Charles C
CC
Applied Biosystems/MDS SCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, Canada. charles.liu@sciex.com
Jong
Ray
R
Covey
Tom
T
D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*