title: Purification and characterization of a phytase from Pseudomonas syringae MOK1.
abstract: A phytase (EC 3.1.3.8) from Pseudomonas syringae MOK1 was purified to apparent homogeneity in two steps employing cation and an anion exchange chromatography. The molecular weight of the purified enzyme was estimated to be 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The optimal activity occurred at pH 5.5 and 40 degrees C. The Michaelis constant (Km) and maximum reaction rate (Vmax) for sodium phytate were 0.38 mM and 769 U/mg of protein, respectively. The enzyme was strongly inhibited by Cu2+, Cd2+, Mn2+, and ethylenediaminetetraacetic acid (EDTA). It showed a high substrate specificity for sodium phytate with little or no activity on other phosphate conjugates. The enzyme efficiently released orthophosphate from wheat bran and soybean meal.
authors: School of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, 441-744, Suweon, Korea.
authors: Jaie Soon
authors: JS
authors: Cho
authors: Chang Whan
authors: CW
authors: Lee
authors: Seung Ha
authors: SH
authors: Kang
authors: Jae Cheon
authors: JC
authors: Lee
authors: Jin Duck
authors: JD
authors: Bok
authors: Yang Soo
authors: YS
authors: Moon
authors: Hong Gu
authors: HG
authors: Lee
authors: Sung Chan
authors: SC
authors: Kim
authors: Yun Jaie
authors: YJ
authors: Choi
mesh_terms: D010832:6-Phytase / Q000302:isolation & purification* / Q000378:metabolism*; D000821:Animal Feed; D002104:Cadmium / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D003300:Copper / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000032:analysis; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000378:metabolism; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D044224:Pseudomonas syringae / Q000201:enzymology*; D013025:Glycine max / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004043:Dietary Fiber; D004791:Enzyme Inhibitors; D010710:Phosphates; D002104:Cadmium; D008345:Manganese; D003300:Copper; D010833:Phytic Acid; D004492:Edetic Acid; D010832:6-Phytase