title: Penetration enhancement in mouse skin and lipolysis in adipocytes by TAT-GKH, a new cosmetic ingredient.
abstract: Since the basic domain of human immunodeficiency virus type I (HIV-1) transactivator of transcription (TAT) protein was reported to possess the ability to traverse biological membranes efficiently, various therapeutic proteins have been attached to TAT for the purpose of therapy. In this study, the tripeptide GKH (glycine-lysine-histidine) derived from parathyroid hormone (PTH), known as lipolytic peptide, was attached to 9-poly lysine (TAT) to be used as a cosmetic ingredient in slimming products. TAT-GKH at 10(-5) M induced approximately 37.6% and 41.5% maximal lipolytic effects in cultured 3T3-L1 differentiated adipocytes and in epididymal adipocytes isolated from rats, respectively, compared with basal lipolysis. The lipolytic effect of GKH was not changed by TAT-GKH fusion. In cytotoxicity tests, there was no cytotoxicity in any dose concentration of TAT-GKH. We confirmed that TAT-GKH induced lipolytic activity by GKH without cytotoxicity and with the possibility of its use as a safe cosmetic ingredient. TAT-GKH elevated penetration into excised hairless mice skin 36 times more efficiently than GKH. TAT-GKH can be used as a cosmetic ingredient in slimming products, with both penetration enhancement and lipolytic effect without cytotoxicity.
authors: Cosmetic R&D Center, LG Household & Healthcare Research Park 84, Jang-dong, Yusong-gu, Taejon 305-343, Korea.
authors: Jun Man
authors: JM
authors: Lim
authors: Min Youl
authors: MY
authors: Chang
authors: Sun Gyoo
authors: SG
authors: Park
authors: Nae Gyu
authors: NG
authors: Kang
authors: Young Sook
authors: YS
authors: Song
authors: Young Hwa
authors: YH
authors: Lee
authors: Young Chang
authors: YC
authors: Yoo
authors: Wan Goo
authors: WG
authors: Cho
authors: Soo Young
authors: SY
authors: Choi
authors: She Hoon
authors: SH
authors: Kang
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003358:Cosmetics / Q000737:chemistry*; D008066:Lipolysis; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*
chemical_list: D003358:Cosmetics; D009842:Oligopeptides; C481804:lysyl-lysyl-lysyl-lysyl-lysyl-lysyl-lysyl-lysyl-lysyl-glycyl-lysyl-histidine