Bradykinin expression in synovial tissues and synovial fluids obtained from patients with internal derangement of the temporomandibular joint.
Bradykinin has been implicated in the pathogenesis of inflammatory arthritis by virtue of the potent pro-inflammatory properties. The purpose of this study is to investigate the expression of bradykinin in patients with internal derangement of the temporomandibular joint (TMJ). We examined 33 TMJ synovial biopsy specimens from 31 patients with internal derangement of the TMJ by an immunohistochemical technique using specific antibodies. We also determined the concentration of bradykinin in 20 synovial fluids from 18 patients with TMJ internal derangement by enzyme-linked immunosorbent assay. These data were compared with those of the control subjects. Bradykinin was predominantly localized in the synovial lining cell layer of TMJ samples obtained from patients with TMJ internal derangement. Bradykinin was also detected in 19 patients' TMJ synovial fluids and the average of bradykinin concentration in the synovial fluids of patients was higher than that of the healthy controls. Although a statistically significant correlation was not observed, these findings support the hypothesis that bradykinin may also be involved in the pathogenesis of TMJ pain and synovitis.
Suzuki
Toshikazu
T
Dept. Oral and Maxillofacial Surgery, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Ishikawa 920-0293, Japan. suzukig3@kanazawa-med.ac.jp
Segami
Natsuki
N
Nishimura
Masaaki
M
Sato
Jun
J
Nojima
Takayuki
T
D000328:Adult; D001920:Bradykinin / Q000032:analysis / Q000096:biosynthesis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D004204:Joint Dislocations / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010147:Pain Measurement; D013582:Synovial Fluid / Q000737:chemistry / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism*; D013705:Temporomandibular Joint Disorders / Q000378:metabolism*
D001920:Bradykinin