Fabrication of glucose oxidase/polypyrrole biosensor by galvanostatic method in various pH aqueous solutions.
The pH effect of pyrrole electropolymerization in the presence of glucose oxidase (GODx) on the performance and characteristic of galvanostatically fabricated glucose oxidase/polypyrrole (Ppy) biosensor is reported. Preparing the GODx/Ppy biosensors in 0.1 M KCl saline solution with various pH containing 0.05 M pyrrole monomer and 0.5 mg/ml GODx at 382 microA/cm2 current density for 100 mC/cm2 film thickness, both the galvanostatic responses and characteristics of these resulted biosensors were obtained. The results revealed that the galvanostatic glucose biosensor fabricated at neutral pH condition exhibited much higher sensitivity than those fabricated at lower or higher pH conditions, and had a good linearity form zero to 10 mM glucose with the sensitivity of 7 nA/mM. Finally, the long-term stability and the kinetic parameters, Michaelis constant and maximum current, of this biosensor were also reported.
Uang
Yuh-Ming
YM
Chemical Engineering Department, Wu-Feng Institute of Technology, Chia-Yi, Taiwan, ROC.
Chou
Tse-Chuan
TC
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; D012996:Solutions; D014867:Water; C067635:polypyrrole; D005949:Glucose Oxidase; D005947:Glucose