title: [Polymorphism analysis of cytochrome C oxidase 1 (COI) gene in Necator americanus collected from 5 provinces in China].
abstract: OBJECTIVE
  To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates.
  
  
  METHODS
  COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced.
  
  
  RESULTS
  The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation occurred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the sequences ranged from 1.34% to 2.18%.
  
  
  CONCLUSIONS
  The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.
authors: Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025.
authors: Tie-hua
authors: TH
authors: Li
authors: Xiang-rong
authors: XR
authors: Guo
authors: Ling
authors: L
authors: Hu
authors: Shu-hua
authors: SH
authors: Xiao
authors: Hai-chou
authors: HC
authors: Xue
authors: John
authors: J
authors: Hawdon
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D017221:Necator americanus / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV