Determination of geosmin, 2-methylisoborneol, and a musty-earthy odor in wheat grain by SPME-GC-MS, profiling volatiles, and sensory analysis.
Geosmin and 2-methylisoborneol-compounds responsible for the musty-earthy off-odor of wheat grain, were isolated by SPME and analyzed by GC-MS. Carboxen/PDMS/DVB fiber coating was selected because of its highest extraction efficiency. Concentrations of geosmin and 2-methylisoborneol as low as 0.001 microg/kg were detected in SIM mode using ion trap mass spectrometer. Apart from GC-MS determination of geosmin and 2-methylisoborneol, various methods for evaluating the musty-earthy off-odor caused by these compounds in wheat grain are presented. Sensory profile analysis differentiated wheat grain into sound and off-flavored, but the method was tedious. Similar groupings, however, were obtained using more rapid methods such as comparison of volatile profiles using SPME-fast GC with PCA projection of data and metal oxide (MOS) based electronic nose.
Jeleń
Henryk H
HH
Institute of Food Technology, The August Cieszkowski Agricultural University of Poznań, 60-624 Poznań, Poland. henrykj@au.poznan.pl
Majcher
Małgorzata
M
Zawirska-Wojtasiak
Renata
R
Wiewiórowska
Małgorzata
M
Wasowicz
Erwin
E
D001892:Camphanes / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009284:Naphthols / Q000032:analysis*; D009812:Odorants / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D012903:Smell; D014908:Triticum / Q000737:chemistry* / Q000382:microbiology; D014835:Volatilization
D001892:Camphanes; D009284:Naphthols; C005536:2-methylisoborneol; C001278:geosmin