title: Inhibition of spinal fusion by use of a tissue ingrowth inhibitor.
abstract: "Spinal instrumentation without fusion" techniques, which do not interfere with spinal growth, have been used extensively in the treatment of progressive spinal scoliosis in very young children. Due to subperiosteal exposure, the process of spinal instrumentation may induce spontaneous bony fusion. Instrumentation and surgical techniques have been modified in order to prevent spontaneous posterior fusion from occurring in children. An absorbable ADCON-L gel has been shown to inhibit scar and epidural adhesions following spinal surgeries. However, little is known about its influence on spinal fusion. In the present study, a single-level intertransverse arthrodesis at L4-5 on both sides was performed on each of nine pigs. Each side was randomly designed to receive autogenous bone graft with or without ADCON-L gel. The animals were followed for 10 weeks postoperatively. A fusion rate of 78% (7/9) was obtained in the autograft treatment by plain X-ray and CT evaluation, while the autograft/ADCON-L treatment yielded a 0% (0/9) fusion rate ( p = 0.001). Histomorphometric evaluation revealed that the addition of ADCON-L gel to bone graft decreased bone and bone marrow formation and significantly increased fibrous tissue formation. No statistical difference between the two treatments was found in cartilage, bone surface density, osteoid surfaces or osteoclast-covered surfaces in any zone. We conclude that ADCON-L gel mixed into autogenous bone graft can delay or decrease bone formation at spinal arthrodesis sites, thus influencing the extent of spinal fusion. This accords with our hypothesis that the use of ADCON-L gel can prevent not only the occurrence of spontaneous fusion in very young scoliosis patients after instrumentation without fusion, but also re-ossification of a decompressed spinal canal.
authors: Orthopaedic Research Laboratory, Orthopaedic Department E, Aarhus University Hospital, Nørrebrogade 44, 8000, Aarhus C, Denmark. zxnong@hotmail.com
authors: Xuenong
authors: X
authors: Zou
authors: Haisheng
authors: H
authors: Li
authors: Niels
authors: N
authors: Egund
authors: Martin
authors: M
authors: Lind
authors: Cody
authors: C
authors: Bünger
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D005260:Female; D005782:Gels / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology; D007796:Laminectomy; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery*; D023421:Models, Animal; D009930:Organic Chemicals; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control; D013122:Spinal Diseases / Q000473:pathology / Q000517:prevention & control; D013123:Spinal Fusion / Q000379:methods*; D034421:Sus scrofa; D014057:Tomography, X-Ray Computed
chemical_list: C104199:ADCON-L; D005782:Gels; D006131:Growth Inhibitors; D009930:Organic Chemicals