Expression of human decay accelerating factor or membrane cofactor protein genes on mouse cells inhibits lysis by human complement.
Mouse cells expressing the human complement regulatory proteins decay accelerating factor (DAF) or membrane cofactor protein (MCP) were produced both by hybridoma technology and by transfection with the appropriate cDNAs. The expression of either or both of these products protected the mouse cell from lysis by human (though not rabbit) complement in the presence of naturally occurring human anti-mouse antibody. This effect could be abrogated by the addition of monoclonal antibody against DAF or MCP. These data suggested that the production of animals transgenic for human complement regulatory proteins should in principle be similarly protected from hyperacute xenograft rejection.
White
D J
DJ
Clinical School of Medicine, University of Cambridge Addenbrooke's Hospital, Hills Rd., Cambridge CB2 2QQ.
Oglesby
T
T
Liszewski
M K
MK
Tedja
I
I
Hourcade
D
D
Wang
M W
MW
Wright
L
L
Wallwork
J
J
Atkinson
J P
JP
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D018958:CD55 Antigens / Q000235:genetics* / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D002878:Chromosomes, Human, Pair 1; D003165:Complement System Proteins / Q000276:immunology*; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D011817:Rabbits; D014162:Transfection; D014183:Transplantation, Heterologous / Q000276:immunology
D015703:Antigens, CD; C497857:CD46 protein, human; D018958:CD55 Antigens; C497859:Mcp protein, mouse; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D003165:Complement System Proteins