Human connective tissue growth factor expressed in Escherichia coli is a non-mitogenic inhibitor of apoptosis.
After cloning and sequencing chicken connective tissue growth factor (cCTGF) we showed that in serum-deprived chicken embryo fibroblasts (CEF) the initial decline of cCTGF mRNA was followed by an increase. Human CTGF that was produced as a soluble protein in Escherichia coli by thioredoxin co-expression inhibited serum-deprivation induced apoptosis of CEF at I(50) of approximately 8pM, demonstrating a function of CTGF at concentrations observed in supernatants of fibroblasts. CTGF was neither mitogenic for CEF nor for other cell types. In conclusion CTGF supports very efficiently survival of certain cells without stimulating their cell growth.
Gygi
Daniel
D
Physiologisches Institut, Cardiovascular Research, Universität Zürich and Cardiology, University Hospital Zürich, Switzerland.
Zumstein
Peter
P
Grossenbacher
Doris
D
Altwegg
Lukas
L
Lüscher
Thomas F
TF
Gehring
Heinz
H
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D015152:Blotting, Northern; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D055513:Connective Tissue Growth Factor; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008934:Mitogens / Q000378:metabolism; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013879:Thioredoxins / Q000378:metabolism; D013997:Time Factors
D001425:Bacterial Outer Membrane Proteins; C526317:CCN2 protein, human; C526318:CCN2 protein, mouse; D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008934:Mitogens; C063931:Omp2 protein, bacteria; D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor; D013879:Thioredoxins