Characterization of a mosquitocidal Bacillus thuringiensis serovar sotto strain isolated from Okinawa, Japan.
OBJECTIVE
To characterize the mosquitocidal activity of parasporal inclusions of the Bacillus thuringiensis serovar sotto strain 96-OK-85-24, for comparison with two well-characterized mosquitocidal strains.


RESULTS
The strain 96-OK-85-24 significantly differed from the existing mosquitocidal B. thuringiensis strains in: (1) lacking the larvicidal activity against Culex pipiens molestus and haemolytic activity, and (2) SDS-PAGE profiles, immunological properties and N-terminal amino acid sequences of parasporal inclusion proteins.


CONCLUSIONS
It is clear from the results that the strain 96-OK-85-24 synthesizes a novel mosquitocidal Cry protein with a unique toxicity spectrum.


CONCLUSIONS
This is the first report of the occurrence of a mosquitocidal B. thuringiensis strain with an unusual toxicity spectrum, lacking the activity against the culicine mosquito.
Ohgushi
A
A
Graduate School of Agriculture, Kyushu University, Fukuoka, Japan.
Wasano
N
N
Shisa
N
N
Saitoh
H
H
Mizuki
E
E
Maeda
M
M
Ohba
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000633:toxicity*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000633:toxicity; D009033:Culicidae / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis; D002479:Inclusion Bodies / Q000737:chemistry / Q000648:ultrastructure; D007300:Insect Control / Q000379:methods*; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010572:Pest Control, Biological / Q000379:methods*; D012756:Sheep; D013171:Spores, Bacterial / Q000737:chemistry / Q000648:ultrastructure
D001426:Bacterial Proteins; D001427:Bacterial Toxins