[Study on gene expression difference of polymorphonuclear neutrophils in early and late periods of systemic inflammatory response syndrome].
OBJECTIVE
To study the gene expression difference of polymorphonuclear neutrophils (PMN) in systemic inflammatory response syndrome (SIRS) in early and late periods termed as multiple organ dysfunction syndrome (MODS) and to evaluate the related changes in function of PMN.


METHODS
Using cDNA microarray technology, RNA of peripheral blood PMN of 4 patients with SIRS was detected in early and late periods, the gene expression difference was observed and analyzed.


RESULTS
Among 8 464 genes there were 84 differently expressed with 19 expressed higher and 65 expressed lower. The differently expressed genes consisted of cell receptor genes (31 percent), immunity-related genes (27 percent), metabolism-related genes (20 percent), and genes of DNA binding or transcriptional factors (18 percent) etc.


CONCLUSIONS
Differently expressed genes suggest that PMN in MODS be in a dysfunctional state characterized by decreasing innate immunity response and increased tissue auto-injury, which may portend a bad prognosis of the patients.
Liu
Yi
Y
Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. ziboliuyi@yeah.net
Deng
Xiao-ming
XM
Li
Jin-bao
JB
D020869:Gene Expression Profiling; D006801:Humans; D009102:Multiple Organ Failure / Q000097:blood*; D009504:Neutrophils / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood*