title: Vestibular Schwann cells are a distinct subpopulation of peripheral glia with specific sensitivity to growth factors and extracellular matrix components.
abstract: Vestibular nerve Schwann cells are predisposed to develop schwannoma. While knowledge concerning this condition has greatly improved, little is known about properties of normal vestibular Schwann cells. In an attempt to understand this predisposition, we evaluated cell density regulation and proliferative features of these cells taken from 6-day-old rats. Data were compared to those obtained with sciatic Schwann cells. In both vestibular and sciatic 7-day-old cultures, Schwann cells appear as bipolar or flattened cells. However, sciatic and vestibular cells greatly differ in other aspects: on poly-L-lysine coating, sciatic cells specifically synthesize myelin basic protein, while expression of P0 mRNAs is restricted to some vestibular cells. Laminin increases sciatic cell density but not that of vestibular cells. Fibronectin selectively enhances the proliferation of vestibular Schwann cells and lacks an effect on sciatic ones. Comparison of cell density changes between sciatic and vestibular cells shows that they are sensitive to two different sets of growth factors. Progesterone and FGF-2 combined with forskolin selectively enhance the cell density of sciatic glia, while IGF-1 and GDNF specifically increase vestibular cell density. Furthermore, BrdU incorporation assays indicate that GDNF is also a mitogen for vestibular cells. Altogether, vestibular Schwann cells display phenotypic features and responsiveness to exogenous signals that are significantly different from sciatic Schwann cells, suggesting that vestibular glia form a subpopulation of Schwann cells.
authors: INSERM U583, Université Montpellier II, Place Eugène Bataillon, 34090 Montpellier, France. sly@univ_montp2.fr
authors: Sylvain
authors: S
authors: Bartolami
authors: Christelle
authors: C
authors: Augé
authors: Cécile
authors: C
authors: Travo
authors: Stéphanie
authors: S
authors: Ventéo
authors: Marlies
authors: M
authors: Knipper
authors: Alain
authors: A
authors: Sans
mesh_terms: D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology*; D012584:Sciatic Nerve / Q000166:cytology; D014725:Vestibular Nerve / Q000166:cytology*
chemical_list: D016326:Extracellular Matrix Proteins; D006133:Growth Substances