The role of hydrophobic interactions in amyloidogenesis: example of prion-related polypeptides.
Conversion of the non-infectious, cellular form of the prion protein (PrP(C)) to the infectious form (PrP(Sc)) is thought to be driven by an alpha-helical to beta-sheet conformational transition. To reveal the sequence determinants which encourage the transition to beta-fold, we study the synthetic peptides associated with hydrophobic conserved fragments of the N-terminal region of the prion protein. The structure of peptides in solution was probed under various thermodynamic conditions employing circular dichroism and steady state fluorescence spectroscopy as well as dye binding assays. The fluorescence methods utilized pyrene residues covalently attached to the end of the model peptides. In aqueous solutions, the structure assessments indicate the formation of metastable peptide aggregates; the molecular conformations within the peptide micelles are largely coiled. This stage in molecular assembly exists without significant beta-strand formation, i.e., before the appearance of any ordered secondary structure detectable by circular dichroism. At moderate concentrations of trifluoroethanol and/or acetonitrile, the conformational ensemble shifts towards beta-strand formation, and the population of the amorphous aggregates decreases significantly. Overall, the present data indicate that hydrophobic interactions between side chains of the peptide variants prevent, in fact, the formation of the rigid beta-sheet structures. Encouragement of beta-folds requires the destabilization of local interactions in the peptide chain, which in vivo might be possible within cell membranes as well as within partly folded molecular forms.
Tcherkasskaya
Olga
O
Department of Biochemistry & Molecular Biology, Georgetown University School of Medicine, Washington DC, USA. ovt@georgetown.edu
Sanders
William
W
Chynwat
Verradej
V
Davidson
Eugene A
EA
Orser
Cindy S
CS
D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000502:physiology*; D000818:Animals; D002942:Circular Dichroism; D004396:Coloring Agents / Q000494:pharmacology; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017096:Prion Diseases / Q000378:metabolism; D011328:Prions / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D014466:Ultraviolet Rays
D000682:Amyloid; D004396:Coloring Agents; D010455:Peptides; D011328:Prions