title: AAF-cmk sensitizes tumor cells to trail-mediated apoptosis.
abstract: TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.
authors: Department of Immunology, Center of Biostructure, The Medical University of Warsaw, Chalubińskiego 5, 02004 Warsaw, Poland.
authors: Izabela
authors: I
authors: Młnarczuk
authors: Paweł
authors: P
authors: Mróz
authors: Grazyna
authors: G
authors: Hoser
authors: Dominika
authors: D
authors: Nowis
authors: Łukasz P
authors: ŁP
authors: Biały
authors: Halina
authors: H
authors: Ziemba
authors: Tomasz
authors: T
authors: Grzela
authors: Wojciech
authors: W
authors: Feleszko
authors: Jacek
authors: J
authors: Malejczyk
authors: Cezary
authors: C
authors: Wójcik
authors: Marek
authors: M
authors: Jakóbisiak
authors: Jakub
authors: J
authors: Gołab
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D020298:U937 Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; C055604:alanyl-alanyl-phenylalanine chloromethyl ketone; D011065:Poly(ADP-ribose) Polymerases