Development and implementation of an electrochemiluminescence immunoassay for the determination of an angiogenic polypeptide in dog and rat plasma.
A quantitative method based on electrochemiluminescence immunoassay for the determination of the angiogenic agent aFGF-S117 has been developed and validated. Two polyclonal antibodies specific to aFGF-S117 and a wild-type aFGF antibody were selected for the analysis. The assay was based on the non-competitive sandwich immunoassay principle in which the drug is trapped with a biotinylated antibody that is immobilized on a streptavidin magnetic particle. The drug is then sandwiched with a ruthenium chelated second antibody. The assay demonstrates good accuracy and reproducibility at plasma concentration of 0.5 ng/ml.
Yuan
A S
AS
Merck Research Laboratories, WP75A-203, West Point, PA 19486, USA. al_yuan@merck.com
Morris
M L
ML
Yin
K C
KC
Hsieh
J Y-K
JY
Matuszewski
B K
BK
D042501:Angiogenic Proteins / Q000097:blood* / Q000737:chemistry; D000818:Animals; D004285:Dogs; D004563:Electrochemistry; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D051381:Rats
D042501:Angiogenic Proteins