Hypermethylation of retrotransposons in the liverwort Marchantia paleacea var. diptera.
Three DNA fragments encoding part of a reverse transcriptase similar to that of Ty1- copia retrotransposons were isolated from suspension-cultured cells of the liverwort Marchantia paleacea var. diptera. Digestion of total DNA with methylation-sensitive and -insensitive isoschizomers revealed that, like those of the subtelomeric region, CpG sequences in retrotransposon genes were hypermethylated. The methylation status and expression of these retrotransposons was less affected by 5-azadeoxycytidine treatment compared to the subtelomeric region, which was considerably demethylated.
Fukuda
T
T
Graduate School of Science and Technology, Kumamoto University, Kurokami, 860-8555 Kumamoto, Japan.
Sakai
M
M
Takano
H
H
Ono
K
K
Takio
S
S
D000595:Amino Acid Sequence; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003470:Culture Media; D019175:DNA Methylation; D018744:DNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D000077209:Decitabine; D015870:Gene Expression; D035926:Hepatophyta / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics; D018626:Retroelements / Q000235:genetics*
D003470:Culture Media; D018744:DNA, Plant; D018626:Retroelements; D000077209:Decitabine; D001374:Azacitidine