Random walks with thin filaments: application of in vitro motility assay to the study of actomyosin regulation.
The in vitro motility devised by Kron and Spudich (Kron and Spudich, 1986; Kron et al., 1991) has proved a very valuable technique for studying the motor properties of myosin of all kinds but it is equally useful for the study of the thin filaments of muscle and their regulation. The movement of a population of thin filaments over immobilised myosin appears to be random but it does in fact yield a large amount of information about contractility and its regulation. The key to extracting useful information from in vitro motility assay experiments is the logical and comprehensive analysis of filament movements.
Marston
Steven
S
Imperial College London, NHLI Doverhouse Street, London SW3 6LY, UK. s.marston@ic.ac.uk
D008841:Actin Cytoskeleton / Q000502:physiology*; D000205:Actomyosin / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000502:physiology; D002148:Calmodulin-Binding Proteins / Q000502:physiology; D009202:Cardiomyopathies / Q000235:genetics / Q000503:physiopathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008840:Microfilament Proteins; D008856:Microscopy, Fluorescence; D009119:Muscle Contraction / Q000235:genetics / Q000502:physiology; D009154:Mutation; D015879:Myosin Subfragments / Q000502:physiology; D014335:Tropomyosin / Q000502:physiology; D014336:Troponin / Q000502:physiology; D000096985:Calponins
D000205:Actomyosin; D002118:Calcium; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D008840:Microfilament Proteins; D015879:Myosin Subfragments; D014335:Tropomyosin; D014336:Troponin; D000096985:Calponins