title: Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii.
abstract: We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.
authors: Northeast Structural Genomics Consortium Center for Advanced Biotechnology and Medicine (CABM), Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA.
authors: James M
authors: JM
authors: Aramini
authors: Yuanpeng J
authors: YJ
authors: Huang
authors: John R
authors: JR
authors: Cort
authors: Sharon
authors: S
authors: Goldsmith-Fischman
authors: Rong
authors: R
authors: Xiao
authors: Liang-Yu
authors: LY
authors: Shih
authors: Chi K
authors: CK
authors: Ho
authors: Jinfeng
authors: J
authors: Liu
authors: Burkhard
authors: B
authors: Rost
authors: Barry
authors: B
authors: Honig
authors: Michael A
authors: MA
authors: Kennedy
authors: Thomas B
authors: TB
authors: Acton
authors: Gaetano T
authors: GT
authors: Montelione
mesh_terms: D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D043902:Pyrococcus horikoshii / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D012269:Ribosomal Proteins; C040469:ribosomal protein S30