title: [Co-expression of CFA/I and CS6 of Enterotoxigenic Escherichia coli ( ETEC ) in Shigella flexneri 2a T32 Derivative Strain FWL01].
abstract: Among the known colonization factors of enterotoxigenic Escherichia coli (ETEC), CFA/I and CS6 (the common antigen in the CFA/IV fimbrial antigens ) are two of the most prevalent fimbriae found in clinical isolates but are never expressed by the same wild-type strains. In this study, CFA/I and CS6 of ETEC were co-expressed in Shigella flexneri 2a T32 derivative strain FWL01 by using a host-plasmid lethal balancing system based on asd gene. The results indicate that the recombinant plasmid carrying CFA/I and CS6 could be stably integrated in FWL01. Expression of the two antigens did not interfere the host growth. The results of immunofluorescence analysis showed that CFA/I and CS6 were localized on the surface of the strain FWL01. In Balb/c mice orally immunized with the recombinant strain, the immune responses against CFA/I and CS6 were observed. Those observations show the feasibility of a multivalent vaccine expressing different fimbrial antigens in attenuated Shigella flexneri.
authors: Beijing Institute of Biotechnology, Beijing 100071, China.
authors: Ji-Ping
authors: JP
authors: Zheng
authors: Ling-Chun
authors: LC
authors: Wang
authors: Peng
authors: P
authors: Wang
authors: Gang
authors: G
authors: Luo
authors: Shu-Qin
authors: SQ
authors: Li
authors: Hai-Qing
authors: HQ
authors: Duan
authors: Cui-Fen
authors: CF
authors: Huang
authors: Zhao-Shan
authors: ZS
authors: Zhang
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012763:Shigella flexneri / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; C098406:CS6 antigen, E coli; D029968:Escherichia coli Proteins; D014613:Vaccines, Attenuated; C022924:colonization factor antigens; D036781:Fimbriae Proteins