title: Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures.
abstract: The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, PR China.
authors: Ming
authors: M
authors: Li
authors: Anton
authors: A
authors: Poliakov
authors: U Helena
authors: UH
authors: Danielson
authors: Zhiguo
authors: Z
authors: Su
authors: Jan-Christer
authors: JC
authors: Janson
mesh_terms: D002845:Chromatography / Q000379:methods*; D004789:Enzyme Activation; D004793:Enzyme Reactivators / Q000737:chemistry; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D004793:Enzyme Reactivators; D007527:Isoenzymes; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases