title: In vivo calibration of the subcutaneous amperometric glucose sensors using a non-enzyme electrode.
abstract: A new two-point calibration method for the subcutaneous amperometric continuous glucose sensor is reported. The proposed method is based on direct measurement of the background current (I(o)) using a non-enzyme electrode. For in vivo test, three electrodes were implanted in rabbits. Two of the three were identical needle-type enzyme electrodes with perfluorinated polymer outer layers (Pt/enzyme layer/Kel-F/PTFE/Kel-F/Nafion) that were placed in subcutaneous tissue and in a vessel (ear artery), respectively. And one non-enzyme electrode with exactly the same membrane composition as those of other two was in the subcutaneous layer to measure the background current. Implantation in the subcutaneous layer generated many crevices on the protecting layers of the electrodes. The signals from enzyme electrodes were effectively corrected by the measured background current from the non-enzyme electrode. In addition, a telemetric monitoring system was developed and evaluated for in vivo continuous glucose monitoring in order to alleviate the problems of motion artifact.
authors: Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 28 Yongon Dong, Chongno Gu, Seoul 110-744, South Korea.
authors: Ran-A
authors: RA
authors: Jeong
authors: Jae Youn
authors: JY
authors: Hwang
authors: Segyeong
authors: S
authors: Joo
authors: Taek Dong
authors: TD
authors: Chung
authors: Sejin
authors: S
authors: Park
authors: Sun Kil
authors: SK
authors: Kang
authors: Won-Yong
authors: WY
authors: Lee
authors: Hee Chan
authors: HC
authors: Kim
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D005240:Feasibility Studies; D008297:Male; D018670:Monitoring, Ambulatory / Q000295:instrumentation* / Q000379:methods; D019736:Prostheses and Implants; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose