[Investigation of interaction of immunoglobulins and detection of viral antigens in cell homogenates by the surface plasmon resonance method].
Immobilization of immunoglobulins on the unmodified gold surface and the gold surface, modified by thiocyanate and protein A Staphylococcus aureus and their subsequent interaction with complementary antibodies were studied using the surface plasmon resonance method. Viral antigens were detected by surface plasmon resonance method in the cell homogenate of green alga Bracteococcus minor, which was artificially infected by tobacco mosaic virus. It was shown, that the process of interaction between virus and antiviral serum obey the Langmure model.
Boltovets'
P M
PM
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Boĭko
V P
VP
Iwe
M
M
Snopok
B A
BA
Shyrshov
Iu M
IuM
Diachenko
N S
NS
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D000460:Chlorophyta / Q000821:virology*; D006046:Gold; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D013045:Species Specificity; D013205:Staphylococcal Protein A; D020349:Surface Plasmon Resonance / Q000379:methods; D013861:Thiocyanates; D014027:Tobacco Mosaic Virus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007136:Immunoglobulins; D013205:Staphylococcal Protein A; D013861:Thiocyanates; D006046:Gold; C031760:thiocyanate