Complete coding sequences of dengue-1 viruses from Paraguay and Argentina.
We have determined the complete coding sequences of six dengue-1 (DEN-1) viruses isolated from Paraguay and Argentina in 2000 from patients with dengue fever. Sequences of strains 259par00, 280par00, 295arg00, 297arg00 and 301arg00 can encode a polyprotein of 3392 amino acids. Strain 293arg00 circulated as a "wild type+deletion mutant" quasispecies, with a subpopulation characterized by a 3-nucleotide deletion in the NS4A region. This variant, which would encode a three amino acid change in the NS4A protein, was found as a minority population in one additional partially-sequenced isolate from the same outbreak. These six South American strains group into two different clades of the "American-African" DEN-1 genotype-one clade is most closely related to strains isolated from Brazil in 1997, the other to a Peruvian strain isolated in 1991 for which only partial sequence information is available. DEN-1 viruses isolated worldwide comprise at least four different genotypes according to previously defined classification criteria.
Avilés
G
G
Department of Microbiology, University of Nevada, Reno, MS 200, Reno, NV 89557, USA. aviles@unr.nevada.edu
Meissner
J
J
Mantovani
R
R
St  Jeor
S
S
D000595:Amino Acid Sequence; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004196:Disease Outbreaks; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010239:Paraguay / Q000453:epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA