[Radioenzymatic investigation of membrane and soluble forms of angiotensin-converting enzyme in acetate-phosphate buffer].
The dose response of soluble and membrane forms of angiotensin-converting enzyme to gamma-irradiation is investigated at different pH values of the medium and at different concentrations of acetate-phosphate buffer. Membrane form of the enzyme is more stable shows principally other conformational equilibrium than the soluble form. "Splitted" activation peaks on the curves of the enzyme dose response are observed.
Orlova
M A
MA
Lomonosov Moscow State University, Chemical Fuculty, Moscow, 119899 Russia. orlova@radio.chem.msu.ru
Kost
O A
OA
Grinshteĭn
S V
SV
Fedoseev
V M
VM
Korzhuev
A V
AV
Troshina
N N
NN
D000085:Acetates; D002021:Buffers; D004307:Dose-Response Relationship, Radiation; D004795:Enzyme Stability / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D008566:Membranes / Q000201:enzymology / Q000528:radiation effects*; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000528:radiation effects*; D010710:Phosphates
D000085:Acetates; D002021:Buffers; D010710:Phosphates; D007703:Peptidyl-Dipeptidase A