title: The relationship between HBV lamivudine resistance and HBV genotypes or basic core promoter mutations.
abstract: OBJECTIVE
  To investigate the relationship between HBV lamivudine resistance and HBV genotypes or basic core promoter (BCP) mutations.
  
  
  METHODS
  The common coated probes were synthesized according to the conserved regions of the preC gene of hepatitis B virus (HBV). Different colorized probes were chosen from the sequences of different genotypes of HBV (A to F), BCP and YMDD wild types and mutants, respectively. HBV DNA levels, HBV genotypes, BCP and YMDD resistants were analyzed by PCR microplate hybridization ELISA at the zero and 6th month after the patients were treated with lamivudine.
  
  
  RESULTS
  HBV genotyping results showed that HBV types B, C, D accounted for about 30%, 36% and 23% patients respectively. Thirteen BCP mutations (type B in 1 patient, type C in 8 and type D in 4) were found before treatment with lamivudine. HBV DNA levels were lower than 100 pg/ml in 2 patients and higher than 100 pg/ml in 11. 9.4% of the HBV patients (5/43; type C in 3 and type D in 2) showed YMDD resistants and 4 BCP mutations at the same time.
  
  
  CONCLUSIONS
  Oral treatment of lamivudine decreases the level of serum HBV DNA. The appearance of HBV YMDD resistants is related to certain HBV genotypes, and most of them are BCP mutations.
authors: Department of Clinical Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine (TCM), Guangzhou 510120, China. zhoudongyao@21cn.com
authors: Dong-Yao
authors: DY
authors: Zhou
authors: Lian-Ying
authors: LY
authors: Lin
authors: Hong
authors: H
authors: Wang
authors: Jian-Sheng
authors: JS
authors: Huang
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002681:China; D024882:Drug Resistance, Viral / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D019259:Lamivudine