-308 Nco I polymorphism of tumour necrosis factor alpha in overweight Caucasians.
We investigated whether a polymorphism in the promoter region of the TNFalpha gene (-308 A/G) is associated with reduced weight loss in obese Australian subjects on an energy restricted diet. 189 healthy subjects and 91 subjects with type II diabetes were genotyped for the -308 Nco I polymorphism using PCR-RFLP techniques. A subset of these subjects (211 females and 45 males), were placed on a 30% energy restricted diet (6200 kJ) for 12 weeks. Subjects were assessed every 2 weeks and changes in body weight, waist circumference and BMI were used as determinants of weight loss. Fasting plasma was analysed for glucose, insulin, lipids and free fatty acids. 64% of subjects were GG homozygotes, 31% were AG heterozygotes and 5% were AA homozygotes. There was no significant difference between the allele frequency in healthy subjects (0.21) and type 2 diabetic patients (0.24). The presence of the -308 A/G polymorphism did not significantly influence initial BMI, the amount of weight lost (GG, 8.1+/-0.65 kg, AG, 6.9+/-0.77 kg, AA, 7.6+/-0.12 kg), waist circumference or any metabolic variable. The AA variant at position -308 in the promoter region of the TNFalpha gene does not influence the amount of weight lost in overweight and obese men and women on a 30% energy restricted diet.
Morris
Alison M
AM
Department of Physiology, Adelaide University, Adelaide, SA 5000, Australia. alison.morris@uchsc.edu
Heilbronn
Leonie K
LK
Noakes
Manny
M
Kind
Karen L
KL
Clifton
Peter M
PM
D001315:Australia; D001483:Base Sequence; D015992:Body Mass Index; D017931:DNA Primers; D015257:DNA-Cytosine Methylases; D003920:Diabetes Mellitus / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004038:Diet, Reducing; D002149:Energy Intake; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012016:Reference Values; D017384:Sequence Deletion / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D015431:Weight Loss / Q000235:genetics; D044465:White People / Q000235:genetics
D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha; C059640:DNA modification methylase NcoI; D015257:DNA-Cytosine Methylases