Optical bio-sniffer for ethanol vapor using an oxygen-sensitive optical fiber.
An optical bio-sniffer for ethanol was constructed by immobilizing alcohol oxidase (AOD) onto a tip of a fiber optic oxygen sensor with a tube-ring, using an oxygen sensitive ruthenium organic complex (excitation, 470 nm; fluorescent, 600 nm). A reaction unit for circulating buffer solution was applied to the tip of the device. After the experiment in the liquid phase, the sniffer-device was applied for gas analysis using a gas flow measurement system with a gas generator. The optical device was applied to detect the oxygen consumption induced by AOD enzymatic reaction with alcohol application. The sensor in the liquid phase was used to measure ethanol solution from 0.50 to 9.09 mmol/l. Then, the bio-sniffer was calibrated against ethanol vapor from 0.71 to 51.49 ppm with good gas-selectivity based on the AOD substrate specificity. The bio-sniffer with the reaction unit was also used to monitor the concentration change of gaseous ethanol by rinsing and cleaning the fiber tip and the enzyme membrane with buffer solution.
Mitsubayashi
Kohji
K
Department of Human and Information Science, School of Information Technology and Electronics, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. mitsu@keyaki.cc.u-tokai.ac.jp
Kon
Takuo
T
Hashimoto
Yuki
Y
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001944:Breath Tests / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry*; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005740:Gases / Q000032:analysis; D055096:Optical Devices; D055100:Optical Fibers; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005740:Gases; D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D010100:Oxygen