Eosinophil cationic protein in human milk is associated with development of cow's milk allergy and atopic eczema in breast-fed infants.
The precise role of leukocytes and mediators in human milk is still unresolved. Eosinophils are uncommonly detected in human milk and their presence has previously been associated with maternal atopy and development of cow's milk allergy (CMA) in the breast-fed infant. The purpose of this study was to examine the levels of eosinophil cationic protein (ECP) in human milk and to compare the levels with development of allergic diseases in breast-fed infants. Altogether 94 breast-feeding mothers (58 atopic, 36 nonatopic) with their babies were prospectively followed from birth for development of CMA or atopic dermatitis. Colostrum and mature milk samples (at 3 mo of lactation), together with mother's peripheral blood samples, were collected. Milk and blood leukocyte content was evaluated with a light microscope. ECP concentration in human milk was measured by commercial UniCAP method. By the end of a 2-y follow-up, 51 mothers had an infant with CMA, 24 had an infant with atopic dermatitis, and 19 had a healthy infant. ECP concentration in milk was under the detection limit (2 microg/L) in all the mothers with a healthy infant, whereas detectable levels were found in 27% of mothers with a CMA infant and in 42% of those with a baby with atopic dermatitis. Measurable ECP in milk was detected in 26% of the atopic and 25% of the nonatopic mothers. Presence of ECP in human milk is associated with development of CMA and atopic dermatitis in the breast-fed infant, but has no direct association with the maternal atopy.
Osterlund
Pamela
P
Department of Dermatology, Helsinki University Central Hospital, Finland. pamela.osterlund@helsinki.fi
Smedberg
Tanja
T
Hakulinen
Arja
A
Heikkilä
Hannele
H
Järvinen
Kirsi-Marjut
KM
D000328:Adult; D000818:Animals; D001798:Blood Proteins / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology*; D047091:Eosinophil Granule Proteins; D005260:Female; D005500:Follow-Up Studies; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008892:Milk / Q000276:immunology*; D008895:Milk, Human / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D011446:Prospective Studies; D012260:Ribonucleases / Q000276:immunology* / Q000378:metabolism
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases