title: Investigation of the photochemical properties and in vitro phototoxic potential of bumetanide.
abstract: The photophysical and photochemical properties of bumetanide, a sulfonamide diuretic drug, have been investigated and the photodegradation of the drug on exposure to UV-B and UV-A radiation monitored by fluorimetric and chromatographic (HPLC) methods. The main photoproducts were isolated by solid-phase extraction and liquid chromatographic procedures, and their structure elucidated by 1H-NMR and mass spectrometry. Bumetanide generates only extremely small quantities of singlet oxygen (1O2) upon irradiation in the presence of oxygen. The in vitro 3T3 N RU phototoxicity test yielded no evidence of phototoxic action.
authors: Dipartimento di Scienze Farmaceutiche, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors: Jessica
authors: J
authors: Fiori
authors: Roberto
authors: R
authors: Ballardini
authors: Patrizia
authors: P
authors: Hrelia
authors: Vincenza
authors: V
authors: Andrisano
authors: Andrea
authors: A
authors: Tarozzi
authors: Vanni
authors: V
authors: Cavrini
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D002034:Bumetanide / Q000737:chemistry* / Q000633:toxicity*; D004232:Diuretics / Q000737:chemistry / Q000633:toxicity; D066298:In Vitro Techniques; D051379:Mice; D018462:Photobiology; D010777:Photochemistry; D014466:Ultraviolet Rays
chemical_list: D004232:Diuretics; D002034:Bumetanide