title: Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface.
abstract: Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.
authors: National Institute of Advanced Industrial Science and Technology, Hyogo, Japan.
authors: Koichi
authors: K
authors: Kato
authors: Kohei
authors: K
authors: Umezawa
authors: Daniel P
authors: DP
authors: Funeriu
authors: Masato
authors: M
authors: Miyake
authors: Jun
authors: J
authors: Miyake
authors: Teruyuki
authors: T
authors: Nagamune
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D018914:Cells, Immobilized / Q000502:physiology*; D004986:Ether / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D004986:Ether; D011092:Polyethylene Glycols