title: Association of cytolethal distending toxin locus cdtB with enteropathogenic Escherichia coli isolated from patients with acute diarrhea in Calcutta, India.
abstract: Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These isolates were identified as being enteropathogenic E. coli (EPEC). In a retrospective study using a probe hybridization assay, 6 of 138 EPEC strains were found to harbor the cdtB locus. cdtB-positive isolates mostly belong to the O86a and O127a serogroups, with the former being associated with higher expression of CDT. Pulsed-field gel electrophoresis profiles showed that the EPEC strains harboring cdtB strains are genetically diverse.
authors: National Institute of Cholera and Enteric Diseases, Indian Veterinary Research Institute, Calcutta, India.
authors: Mritunjay
authors: M
authors: Pandey
authors: Asis
authors: A
authors: Khan
authors: Suresh C
authors: SC
authors: Das
authors: Bhaswati
authors: B
authors: Sarkar
authors: Soumen
authors: S
authors: Kahali
authors: Subhra
authors: S
authors: Chakraborty
authors: Santanu
authors: S
authors: Chattopadhyay
authors: Shinji
authors: S
authors: Yamasaki
authors: Yoshifumi
authors: Y
authors: Takeda
authors: G Balakrish
authors: GB
authors: Nair
authors: T
authors: T
authors: Ramamurthy
mesh_terms: D000208:Acute Disease; D001427:Bacterial Toxins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D017931:DNA Primers; D003681:Dehydration / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000097:blood / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D009780:Occult Blood; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012703:Serotyping / Q000379:methods; D014839:Vomiting / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D017931:DNA Primers; D029968:Escherichia coli Proteins; C067044:cytolethal distending toxin