title: Screening of augmenter of liver regeneration-binding proteins by yeast-two hybrid technique.
abstract: OBJECTIVE
  To investigate the biological function of augmenter of liver regeneration (ALR), we used yeast-two hybrid technique to detect proteins in hepatocytes interacting with ALR.
  
  
  METHODS
  ALR bait plasmid was constructed by using yeast-two hybrid system 3, then transformed into yeast AH109. The transformed yeast was mated with yeast Y187 containing liver cDNA library plasmid in a 2XYPDA medium. Diploid yeast was plated on a synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing x-alpha-gal for selection and screening. After extracting and sequencing of the plasmid from blue colonies. Analysis was performed by bioinformatics.
  
  
  RESULTS
  Of 36 colonies sequenced, 14 are metallothionein, 12 albumin, and 3 selenoprotein P. One colony is a new gene with unknown function.
  
  
  CONCLUSIONS
  The successful cloning of gene of ALR interacting protein has paved the way for studying the physiological function of ALR and associated proteins.
authors: Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China. cj@genetherapy.com.cn
authors: Jun
authors: J
authors: Cheng
authors: Lin
authors: L
authors: Wang
authors: Ke
authors: K
authors: Li
authors: Yin-Ying
authors: YY
authors: Lu
authors: Gang
authors: G
authors: Wang
authors: Yan
authors: Y
authors: Liu
authors: Yan-Wei
authors: YW
authors: Zhong
authors: Hui-Juan
authors: HJ
authors: Duan
authors: Yuan
authors: Y
authors: Hong
authors: Li
authors: L
authors: Li
authors: Ling-Xia
authors: LX
authors: Zhang
authors: Ju-Mei
authors: JM
authors: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods; D015723:Gene Library; D022781:Hepatocytes / Q000502:physiology*; D006824:Hybridization, Genetic / Q000502:physiology; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology; D008403:Mass Screening / Q000379:methods*; D011506:Proteins / Q000235:genetics*; D015003:Yeasts; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D002352:Carrier Proteins; D011506:Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors