Increase in plasma and surface CD163 levels in patients undergoing coronary artery bypass graft surgery.
Although haptoglobin polymorphism has been shown to be a genetic risk factor in coronary artery disease, its mechanisms of action are incompletely defined. Recently, a macrophage scavenger receptor for the uptake of haptoglobin-hemoglobin (Hp-Hb) complexes was cloned and designated CD163. Macrophage expression of CD163 is increased by glucocorticoids, IL-10 and IL-6. To better understand the in vivo response of CD163 to an inflammatory stimulus and glucocorticoid treatment, we studied 18 patients who underwent elective coronary artery bypass graft (CABG) surgery with cardiopulmonary bypass (CPB). We report a rapid increase in plasma levels of soluble CD163 by 1 h post-declamping the aorta during CABG surgery with CPB. Furthermore, we demonstrate significant increases in monocyte CD163 on post-operative day 1; 14-fold for patients pre-treated with methylprednisolone and 3-fold for those who did not receive exogenous glucocorticoids. These findings show CD163 to be rapidly mobilized in response to systemic inflammatory stimuli and to be affected significantly by glucocorticoids in vivo. The proposed role of CD163 as a Hp-Hb scavenger and anti-inflammatory molecule, in conjunction with the results of this study, make CD163 an intriguing target for potential manipulation of the acute response to inflammation.
Goldstein
Jonathan I
JI
Department of Physiology, Dartmouth Medical School, Hanover, NH 03755, USA.
Goldstein
Katharine A
KA
Wardwell
Kathleen
K
Fahrner
Scott L
SL
Goonan
Katie E
KE
Cheney
Matthew D
MD
Yeager
Mark P
MP
Guyre
Paul M
PM
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis* / Q000097:blood; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis* / Q000097:blood; D000954:Antigens, Surface / Q000096:biosynthesis*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass / Q000009:adverse effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000097:blood; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology / Q000378:metabolism; D000098902:CD163 Antigen
D000209:Acute-Phase Proteins; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D000098902:CD163 Antigen; D005938:Glucocorticoids; D011956:Receptors, Cell Surface; D008775:Methylprednisolone