Effects of fat-soluble extracts from vegetable powder and beta-carotene on proliferation and apoptosis of lung cancer cell YTMLC-90.
OBJECTIVE
The aim of this investigation was to study the effects of fat-soluble extracts from vegetable powder (FEFVP) and beta-carotene on the proliferation and apoptosis of cultured YTMLC-90 lung cancer cells.


METHODS
The lung cancer cells were continuously exposed to a broad range of concentration of FEFVP and beta-carotene. The proliferation was evaluated in MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, and DNA content analysis combined with flow cytometric analysis.


RESULTS
Both FEFVP and beta-carotene were found to inhibit cell proliferation and to induce morphologic changes consistent with apoptosis in YTMLC-90 cancer cells, including cellular shrinkage, chromatin condensation and nuclear fragmentation. DNA agarose gel electrophoresis showed DNA fragmentation 'ladder'. Flow cytometric analysis revealed decreased DNA content and the presence of a sub-G1 apoptotic peak.


CONCLUSIONS
These findings are consistent with the induction of apoptosis. Moreover, the effects of FEFVP are stronger than those of beta-carotene. FEFVP inhibits the growth of YTMLC-90 probably via the induction of apoptosis cancer cells.
Lu
Quan-Jun
QJ
Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450052, Henan, China. lqjnutr@zzu.edu.cn
Huang
Cheng-Yu
CY
Yao
Shu-Xiang
SX
Wang
Rui-Shu
RS
Wu
Xiao-Na
XN
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D002455:Cell Division; D004249:DNA Damage; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D011208:Powders; D014407:Tumor Cells, Cultured; D014675:Vegetables / Q000737:chemistry; D019207:beta Carotene / Q000494:pharmacology*
D000975:Antioxidants; D010936:Plant Extracts; D011208:Powders; D019207:beta Carotene