PFGE and pertactin gene sequencing suggest limited genetic variability within the Finnish Bordetella parapertussis population.
The outer-membrane protein pertactin (Prn) of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica is believed to function as an adhesin and is an important immunogen. The emergence of B. pertussis and B. bronchiseptica Prn variants has been reported. The aim of this study was to determine whether similar variation is found in B. parapertussis Prn and to characterize Finnish clinical B. parapertussis isolates that were collected in 1982-2000. Of 76 B. parapertussis isolates studied, seven (9 %) were found to have silent and non-silent nucleotide changes. In addition, one (1 %) had eight PQP repeats instead of nine. Three closely related B. parapertussis XbaI PFGE patterns were found. Genetic variation of B. parapertussis was found to be very limited, suggesting that B. parapertussis is a stable organism that is well-adapted to its own ecological niche.
Mäkinen
Johanna
J
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
Mertsola
Jussi
J
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
Soini
Hanna
H
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
Arvilommi
Heikki
H
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
Viljanen
Matti K
MK
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
Guiso
Nicole
N
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
He
Qiushui
Q
National Public Health Institute, Department of Human Microbial Ecology and Inflammation1 and Turku Graduate School of Biomedical Sciences, University of Turku2, Turku, Finland 3Department of Pediatrics, Turku University Hospital, Turku, Finland 4Department of Medical Microbiology, University of Turku, Turku, Finland 5Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D042483:Bordetella parapertussis / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010566:Virulence Factors, Bordetella / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin