Applicability of gradient liquid chromatography with tandem mass spectrometry to the simultaneous screening for about 100 pesticides in crops.
A method was developed for screening crops for a range of pesticide residues by liquid chromatography/tandem mass spectrometry (LC/MS/MS). A complete set of LC, electrospray ionization (ESI), and tandem MS acquisition parameters was established for the determination of 108 analytes; these parameters were used for the simultaneous acquisition of 98 analytes in the positive ESI mode and 10 analytes in an additional MS/MS method in the negative ESI mode. The entire procedure involves extraction of residues with methanol-water and partition into dichloromethane. The utility of the method is demonstrated by the analysis of crops of 5 matrix types (water-containing, acidic, dry, sugar-containing, and fatty). Of 108 pesticides/metabolites tested, 104 showed sufficient stability in most matrixes for determination by LC/MS/MS. These analytes belong to 20 chemical classes, which demonstrate the general applicability of the method for multiclass analysis. By using matrix-matched standards, 67 compounds could be determined in most matrixes with recoveries of 70-120% and a relative standard deviation of < or = 25% at the 0.01 mg/kg level.
Klein
Jeannette
J
Federal Institute for Risk Assessment, POB 33 00 13, D-14191 Berlin, Germany.
Alder
Lutz
L
D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D004355:Drug Stability; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol; D008752:Methylene Chloride; D010573:Pesticide Residues / Q000032:analysis*; D011786:Quality Control; D012680:Sensitivity and Specificity; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D010573:Pesticide Residues; D012997:Solvents; D008752:Methylene Chloride; D000432:Methanol