title: Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish.
abstract: The biological functions of insulin-like growth factor (IGF) I and II are modulated by a family of IGF-binding proteins (IGFBPs) in complex IGF-dependent and IGF-independent pathways. For further understanding of the actions of IGFs, some of these binding proteins have been cloned and characterized. We report the molecular cloning of IGFBP-3 cDNA for zebrafish. The tissue-specific and developmental stage-specific expression of IGFBP-3 and the hormonal regulation of its expression have also been determined by comparative reverse transcription polymerase chain reaction. Zebrafish IGFBP-3 cDNA contains an open reading frame of 879 bp, encoding a polypeptide of 293 amino acid residues. Results of this analysis revealed high levels of IGFBP-3 messenger RNA in ovary and fin tissue. Expression of IGFBP-3 mRNA was throughout the entire embryonic development, with the highest level of expression observed at 36 hours after the onset of development. Elevated levels of expression of IGFBP-3 were observed 24 hours after injection with IGF-I and 48 hours with IGF-II or insulin. These results suggest that the expression of IGFBP3 gene might be modulated by IGF-I, IGF-II, and insulin.
authors: Institute of Zoology, Academia Sinica, Nankang, Taipei, Taiwan, ROC.
authors: Jyh-Yih
authors: JY
authors: Chen
authors: Jian-Chyi
authors: JC
authors: Chen
authors: Wei-Tung
authors: WT
authors: Huang
authors: Cheng-Wen
authors: CW
authors: Liu
authors: Cho-Fat
authors: CF
authors: Hui
authors: Thomas T
authors: TT
authors: Chen
authors: Jen-Leih
authors: JL
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013002:Somatomedins / Q000494:pharmacology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D013002:Somatomedins