Optimization of alkyl ester production from grease using a phyllosilicate sol-gel immobilized lipase.
Simple alkyl ester derivatives of restaurant grease were prepared using a lipase from Pseudomonoas cepacia immobilized within a phyllosilicate sol-gel matrix as biocatalyst. Alcoholysis reactions of grease were carried out in solvent-free media using a one-step addition of alcohol to the reaction mixture. The immobilized lipase was active from 40 to 70 degrees C. Ester yields (60-97%) were highest when using a ratio of reactants of 2 mmol grease to 8 mmol alcohol and the biocatalyst was 10% (w/w) of grease in the presence of molecular sieves.
Hsu
An-Fei
AF
US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. ahsu@arserrc.gov
Jones
Kerby C
KC
Foglia
Thomas A
TA
Marmer
William N
WN
D000478:Alkylation; D003246:Conservation of Energy Resources; D004800:Enzymes, Immobilized / Q000096:biosynthesis / Q000737:chemistry; D004951:Esterification; D004952:Esters / Q000138:chemical synthesis*; D000431:Ethanol / Q000737:chemistry*; D005230:Fatty Acids, Nonesterified / Q000737:chemistry*; D005742:Gasoline; D005782:Gels / Q000737:chemistry; D007220:Industrial Waste / Q000517:prevention & control*; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011786:Quality Control; D017640:Silicates / Q000737:chemistry*
D004800:Enzymes, Immobilized; D004952:Esters; D005230:Fatty Acids, Nonesterified; D005742:Gasoline; D005782:Gels; D007220:Industrial Waste; D010938:Plant Oils; D017640:Silicates; D000431:Ethanol; D008049:Lipase