title: Expression of transforming growth factor-beta and insulin-like growth factor in molar and placental tissues.
abstract: The semiquantitative reverse transcription polymerase chain reaction was employed to detect the expression of transforming growth factor beta (TGF-beta) and insulin-like growth factor (IGF) in complete hydatidiform mole, normal first-trimester villi, the normal term placenta (after vaginal/abdominal deliver) and the preeclamptic placenta at term. The expression of IGF-I mRNA was seen in all five tissues, but its level was much lower in the term placental tissues with preeclampsia than in other tissues. The content of IGF-I mRNA in villous tissues from molar pregnancy was slightly higher than in normal first-trimester villi. IGF-II mRNA was detected at similar levels in all three sorts of term placental tissues. However, the expression level of IGF-II mRNA in tissues of complete molar pregnancy was significantly lower than in normal first-trimester villi. TGF-beta(3) was found expressed in all five tissues, while TGF-beta(1) and TGF-beta(2) mRNA were not detected. Compared to the normal first-trimester villi, the expression of TGF-beta(3) in complete hydatidiform molar tissues was comparatively higher. Furthermore, the expression levels of TGF-beta(3) in the preeclamptic placenta and the normal placenta after cesarean birth were higher than in the placenta after vaginal delivery. We concluded that, the change of TGF-beta and IGF expression in placental tissues might be involved in the development of trophoblastic diseases of pregnancy.
authors: Department of Obstetrics and Gynecology, Nanfang Hospital, Guangzhou 510515, China. LAB973@fimmu.edu.cn
authors: Zhan-Jun
authors: ZJ
authors: Pang
authors: Fu-Qi
authors: FQ
authors: Xing
mesh_terms: D000328:Adult; D002824:Chorionic Villi / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000276:immunology / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D010920:Placenta / Q000276:immunology / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II