title: [A comparative study utilizing different methods for establishing an experimental periodontitis model in rats].
abstract: OBJECTIVE
  To evaluate the experimental periodontitis in rats induced by ligature plus high sugar water with and without infection of periodontopathic bacteria.
  
  
  METHODS
  Forty SD rats (5 weeks of age) were randomly divided into a control group (N) and three experimental groups (P1, P2 and P3). All experimental groups were given silk ligatures around the cervix of the second maxillary molars and were provided with drinking water containing 10% glucose. Group P2 was inoculated with P. gingivalis, three times; group P3 was inoculated with P. gingivalis and F. nucleatum, three times. At weeks 0, 2 and 4, microbiological and histological examinations were made; alveolar bone loss was stereoscopically measured as the distance from the cement-enamel junction to the alveolar crest.
  
  
  RESULTS
  Microbiological examination indicated that the bacteria inoculated in groups P2 and P3 were successfully colonized and both of P. gingivalis and F. nucleatum were predominant among the bacterial colonies of the examined sites. At week 2, histological examination showed gingival erosion and alveolar bone loss in all experimental groups and periodontal pockets in groups P2 and P3. The most serious lesions were observed in group P3. At week 4, the periodontal destruction in all experimental groups became more severe.
  
  
  CONCLUSIONS
  Ligature, high sugar water combined with mixed infection of periodontopathic bacteria can successfully induce experimental periodontitis in rats.
authors: Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Yafei
authors: Y
authors: Wu
authors: Xiaoqin
authors: X
authors: Zhao
authors: Yu
authors: Y
authors: Chen
authors: Xinping
authors: X
authors: Fan
authors: Xiaorong
authors: X
authors: Xiao
authors: Zhu
authors: Z
authors: Zhu
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections; D002241:Carbohydrates; D004195:Disease Models, Animal; D008026:Ligation; D010518:Periodontitis / Q000209:etiology; D016966:Porphyromonas gingivalis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002241:Carbohydrates