title: Mucosal co-application of lactic acid bacteria and allergen induces counter-regulatory immune responses in a murine model of birch pollen allergy.
abstract: Recent epidemiological studies and clinical trials suggest a possible role of certain lactic acid bacteria (LAB) strains in the prevention of allergic diseases. In this study, we aimed at evaluating the immunomodulatory potential of two LAB strains, Lactococcus lactis and Lactobacillus plantarum, for prophylaxis and therapy of allergic immune responses. Both LAB strains-induced high levels of IL-12 and IFN-gamma in naive murine spleen cell cultures. Intranasal co-application with recombinant Bet v 1, the major birch pollen allergen, prior or after allergic sensitization, led to increased levels of allergen-specific IgG2a antibodies and in vitro IFN-gamma production, indicating a shift towards Th1 responses. Successful immunomodulation by the mucosal pre-treatment was further demonstrated by suppression of allergen-induced basophil degranulation. We conclude that these LAB strains in combination with an allergen could be promising candidates for mucosal vaccination against type I allergy.
authors: Department of Pathophysiology, Medical School AKH EBO3Q, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
authors: Andreas
authors: A
authors: Repa
authors: Corinne
authors: C
authors: Grangette
authors: Catherine
authors: C
authors: Daniel
authors: Romana
authors: R
authors: Hochreiter
authors: Karin
authors: K
authors: Hoffmann-Sommergruber
authors: Josef
authors: J
authors: Thalhamer
authors: Dietrich
authors: D
authors: Kraft
authors: Heimo
authors: H
authors: Breiteneder
authors: Annick
authors: A
authors: Mercenier
authors: Ursula
authors: U
authors: Wiedermann
mesh_terms: D000276:Adjuvants, Immunologic; D000485:Allergens / Q000276:immunology*; D000818:Animals; D015549:Basophil Degranulation Test; D029662:Betula / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D007111:Immunity, Cellular / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000276:immunology*; D013294:Lactococcus lactis / Q000276:immunology*; D015471:Leukemia, Basophilic, Acute / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000276:immunology*; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D018664:Interleukin-12; D019344:Lactic Acid; D007371:Interferon-gamma