Clinical significance of TGF- beta1 and beta-glucuronidase synchronous detection in human pancreatic cancer.
OBJECTIVE
To investigate the relation of transfer growth factor (TGF-beta1) and beta-glucuronidase (beta-GCD) on the occurrence and progress of pancreatic cancer.


METHODS
The expression of TGF-beta1 and beta-GCD in the pancreatic cancer tissue and normal pancreatic tissue was determined synchronously using ABC method of immunohistochemistry.


RESULTS
The percentage of TGF-beta1 positive cells was significantly higher in pancreatic cancer tissue (43.8%+/-5.2%) than in adjacent pancreatic tissue (28.7%+/-3.6%, P<0.01). The worse the cancer cells differentiated and lymph nodes metastasis, the more over-expression of TGF-beta1. The percentage of beta-GCD positive cells was also significantly higher in the pancreatic cancer tissue (62.5%+/-4.1%) than in the adjacent pancreatic tissue (33.5%+/-2.8%, P<0.01). The degree of over-expression of beta-GCD was related to the degree of cancer cells differentiation, but not to the lymph nodes metastasis. The expression of TGF-beta1 was significantly correlated with the expression of beta-GCD in pancreatic cancer tissue.


CONCLUSIONS
The genesis of pancreatic cancer results from multi-factor, multi-step and multi-gene variation. The synchronous detection of TGF-beta1 and beta-GCD helps to determine the malignant degree of tumors and the prognosis of patients with such disease.
Yue
Hui
H
Department of Digestive Diseases, People's Hospital, Wuhan University, Wuhan 430071, China. kyk567@263.net
Yang
Bo
B
Zhang
Hong
H
Zhu
Shan-De
SD
Du
Xiao-Ju
XJ
Feng
Xin-Li
XL
Yu
Zhong
Z
Xia
Yu-Ting
YT
Yu
Jie-Ping
JP
D002454:Cell Differentiation; D018450:Disease Progression; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D005966:Glucuronidase