Quick preparations of human parvovirus B19 microarray probes using PCR.
OBJECTIVE
To prepare DNA microarray probes for the detection of human parvovirus B19. Method Specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome. The PCR products were cloned into the pMD-18 T vector.


METHODS
Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19.


RESULTS
Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19.


CONCLUSIONS
PCR amplification of the conserved and specific human parvovirus B19 genes is simple and effective to prepare the desired probes.
Lü
Liang
L
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Wang
Hong-min
HM
Ma
Xiao-dong
XD
Sun
Zhao-hui
ZH
Zheng
Wen-ling
WL
D015342:DNA Probes; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016732:Parvovirus B19, Human / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes