title: Renin and kallikrein in connecting tubule of mouse.
abstract: BACKGROUND
  The observation of renin expression in connecting tubule, a segment that also expresses tissue kallikrein (KLK-1), raises two questions. Are the genes expressed in the same or in different cells of connecting tubule? Does this topography support the hypothesis that KLK-1 activates prorenin or is it more likely that it affords coordinated gene regulation?
  
  
  METHODS
  Renin and KLK-1 were examined by immunostaining and in situ hybridization. Renin activation by KLK-1 was investigated in vitro. In vivo, excretion of prorenin and active renin was compared in mice homozygous for targeted inactivation of KLK-1 (TK(-/-)) and normal littermates (TK(+/+)).
  
  
  RESULTS
  Using in situ immunostaining for renin and in situ hybridization for KLK-1 mRNA, we found that connecting tubule cells expressing renin also expressed KLK-1. We confirmed in vitro activation of prorenin by KLK-1, but found no difference in the ratio of active renin to prorenin in urine of TK(-/-) and TK(+/+) animals. Compared to TK(+/+) controls, TK(-/-) mice exhibited significantly lower 24-hour excretion of prorenin (5.05 +/- 1.16 mg Ang I/hour vs. 9.39 +/- 1.96 mg Ang I/hour, P < 0.05) and active renin (1.98 +/- 0.25 mg Ang I/hour vs. 3.58 +/- 0.39 mg Ang I/hour, P < 0.05), with no difference in either urine volumes or plasma renin concentrations.
  
  
  CONCLUSIONS
  Direct interaction between renin and KLK-1, not ruled out in vitro, is not supported in vivo. By contrast, lower excretion of active renin and prorenin in TK(-/-) compared to TK(+/+) suggest coordinated regulation of the two proteins in their participation to collecting duct function.
authors: Department of Human Genetics, University of Utah, Health Sciences Center, Salt Lake City, Utah 84112, USA.
authors: Andreas
authors: A
authors: Rohrwasser
authors: Tomoaki
authors: T
authors: Ishigami
authors: Barbu
authors: B
authors: Gociman
authors: Pierre
authors: P
authors: Lantelme
authors: Terry
authors: T
authors: Morgan
authors: Tong
authors: T
authors: Cheng
authors: Elaine
authors: E
authors: Hillas
authors: Shuhua
authors: S
authors: Zhang
authors: Kenneth
authors: K
authors: Ward
authors: May
authors: M
authors: Bloch-Faure
authors: Pierre
authors: P
authors: Meneton
authors: J M
authors: JM
authors: Lalouel
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012083:Renin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D020840:Tissue Kallikreins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D020840:Tissue Kallikreins; D012083:Renin