title: Cytomegalovirus infection in liver transplant recipients.
abstract: OBJECTIVE
  To explore cytomegalovirus (CMV) infection in recipients of liver transplantation (LT).
  
  
  METHODS
  30 recipients of LT were screened for the appearance of CMV infection by using ELISA to test anti-CMV-Ab from serum samples and using immunohistochemistry method to test CMV antigen expression and nested-PCR to amplify CMV-DNA from blood samples.
  
  
  RESULTS
  Four of 243 samples taken from 30 recipients came out positive of anti-CMV IgG and anti-CMV IgM with a positive rate of 100% and 1.6% respectively. 85 samples resulted in CMV antigen expression (35.0%) with the average antigen index being 4.2+/-3.1/5 X 10(4) WBC. Besides, 99 samples were found to be positive by nested-PCR with a positive rate of 40.7%. 61 samples were found to be simultaneously positive in test of CMV antigen and DNA, with a rate of 25.1%.
  
  
  CONCLUSIONS
  Infection of CMV is common in recipients of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplantation is very important for early diagnosis of CMV infection.
authors: Institute of Infectious Diseases and Key Laboratory of Viral Infectious Diseases, Ministry of Health, Zhejiang University School of Medicine, Hangzhou 310003, China. fjjc@yeh.net
authors: Jun
authors: J
authors: Fan
authors: Wei-Hang
authors: WH
authors: Ma
authors: Jing
authors: J
authors: Qian
authors: Zhi
authors: Z
authors: Chen
authors: Dong-Sheng
authors: DS
authors: Huang
authors: Wei-Lin
authors: WL
authors: Wang
authors: Shu-Sen
authors: SS
authors: Zheng
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016031:Liver Transplantation; D008297:Male; D011183:Postoperative Complications / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M