Determination of specific interactions between glucose ligand carrying polymer and glucose transporter type-1 (GLUT-1) using different cell types.
In order to develop a biomimetic polymer for cell recognition, poly [3-O-(4'-vinylbenzyl)-D-glucose] (PVG) and different types of glucose transport (GLUT)-carrying cells, namely, HepG2 cells (GLUT-1), 3T3-L1 fibroblast cells (GLUT-1 and GLUT-4), and MIN6 cells (GLUT-2), were tested for specific interaction. To clarify the nature of interaction between PVG and the different cell types, rhodamine-B isothiocyanate (RITC)-labeled polymers were used to prove and visualize the interactions. In this study, we found that labeled polymer strongly binds to HepG2 cells. The fluorescence intensity of PVG with in the presence of HepG2 cells was found to be stronger than that of 3T3-L1 fibroblast cells (0.11 +/- 0.05) and MIN6 cells, which carry GLUT-2 (0.028 +/- 0.01); a confocal laser microscopic study confirmed this interaction. To confirm the specificity of the interaction mediated by GLUT, the cells were pretreated with phloretin, an inhibitor of GLUT-1, before adding RITC-labeled PVG polymer to the cell culture medium. This treatment suppressed the interaction of PVG with HepG2 cells and partially suppressed its interaction with 3T3-L1 fibroblast cells.
Chung
Jong-Hoon
JH
Department of Biosystems and Agricultural Engineering, Chonnam National University, Kangjoo, Korea.
Park
Keun-Hong
KH
Seo
Byong-Moo
BM
Kim
Eun-Seok
ES
Hong
Jong-Rak
JR
Chung
Il-Hyuk
IH
Kang
Nara
N
Baek
Jeong-Hwa
JH
Min
Byung-Moo
BM
Choung
Yun-Hoon
YH
Akaike
Toshihiro
T
Choung
Pill-Houng
PH
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005453:Fluorescence; D005947:Glucose; D051272:Glucose Transporter Type 1; D006801:Humans; D008024:Ligands; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D008024:Ligands; D009004:Monosaccharide Transport Proteins; D011137:Polystyrenes; C495677:SLC2A1 protein, human; C495678:Slc2a1 protein, mouse; C473338:poly(3-O-(4'-vinylbenzyl)-D-glucose); D005947:Glucose