Microdevice for separation and quantitative fraction collection.
A new microfluidic concept for quantitative whole-column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD-like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole-column fraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes.
Spesný
Michal
M
Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic.
Foret
Frantisek
F
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D010455:Peptides; D011506:Proteins; D004247:DNA