Effect of dodecyl maltoside detergent on rhodopsin stability and function.
Detergent-solubilized bovine rhodopsin produces mixed detergent/lipid/protein micelles. The effect of dodecyl maltoside detergent on the thermal stability of dark-state rhodopsin, and upon formation of the different intermediates after rhodopsin photobleaching (metarhodopsin II and metarhodopsin III), and upon transducin activation has been studied. No significant effect is observed for the thermal stability of dark-state rhodopsin in the range of detergent concentrations studied, but a decrease in the stability of metarhodopsin II and an increase in metarhodopsin III formation is observed with decreasing detergent concentrations. The transducin activation process is also affected by the presence of detergent indicating that this process is dependent on the lipid micro-environment and membrane fluidity, and this stresses the importance of the native lipid environment in rhodopsin normal function.
Ramon
Eva
E
Centre de Biotecnologia Molecular (CEBIM), Seccio de Terassa, Departament d'Enginyeria Qumica, Universitat Politècnica de Catalunya, Colom 1, 08222 Terrassa, Catalonia, Spain.
Marron
Jordi
J
del Valle
Luis
L
Bosch
Laia
L
Andrés
Anna
A
Manyosa
Joan
J
Garriga
Pere
P
D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D008823:Micelles; D012243:Rhodopsin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000502:physiology; D015293:Transducin / Q000378:metabolism
D003902:Detergents; D005960:Glucosides; D008823:Micelles; C013110:metarhodopsins; C040358:dodecyl maltoside; D012243:Rhodopsin; D019204:GTP-Binding Proteins; D015293:Transducin