Increased serum malondialdehyde and decreased nitric oxide within 24 hours of thrombotic stroke onset.
OBJECTIVE
Ischemia/reperfusion generates free oxygen radicals, which react with the unsaturated lipids of biomembranes resulting in the generation products such as malondialdehyde. Malondialdehyde could be a sensor for tissue damage and reperfusion. Nitric oxide, released due to the early arrival of leukocytes in the brain parenchyma, could be a sensor for nonflow phenomenon. Thereby, the purpose of this research was to evaluate the behavior of malondialdehyde and nitric oxide within the 24 hours after the stroke onset.


METHODS
Fifteen patients up to an age of 49 years, admitted to the emergency of University Hospital and Chiquinquirá Hospital in Maracaibo, Venezuela, were examined by a neurologist and underwent 12-lead electrocardiograms and computed tomography for the diagnosis of thrombotic stroke. Serum malondialdehyde and nitric oxide were measured as thiobarbituric acid adducts and total nitrites. Data were collected within the 24 hours after the stroke onset.


RESULTS
Malondialdehyde for patients with stroke had a significant increase (P<0.001) when compared with healthy controls (47.9 +/- 7.1 vs. 1.7 +/- 0.2 micromol/L). Conversely, serum nitric oxide for patients with stroke had a significant decrease (P<0.001) when compared with the control group (14.5 +/- 1.4 vs. 41.3 +/- 3.7 micromol/L). The lowest values of malondialdehyde and the highest values of nitric oxide were observed in two patients, who died.


CONCLUSIONS
Serum levels of malondialdehyde increase, and serum levels of nitric oxide diminish within 24 hours after the onset of thrombotic stroke onset. This suggests that serum malondialdehyde level could be used as potentially reliable and sensitive marker for reperfusion, whereas nitric oxide levels could acts as potential biochemical sensor for nonreflow phenomenon.
Cano
Climaco P
CP
Centro de Investigaciones Endocrino-Metabólicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela. climacoc@hotmail.com
Bermúdez
Valmore P
VP
Atencio
Hernán E
HE
Medina
Mayerlim T
MT
Anilsa
Amell
A
Souki
Aida
A
Molina
Omaira M
OM
Restrepo
Helen
H
Vargas
Maria E
ME
Núñez
Maryluz
M
Ambard
Merlyn
M
Toledo
Abdón
A
Contreras
Freddy
F
Velasco
Manuel
M
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020767:Intracranial Thrombosis / Q000097:blood* / Q000150:complications; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D012016:Reference Values; D020521:Stroke / Q000097:blood* / Q000209:etiology; D013997:Time Factors; D014687:Venezuela
D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde