[Expression of matrix metalloproteinase 2 and 9 in liver tissue of patients with schistosomiasis japonica].
OBJECTIVE
To explore the change of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in liver tissue of the patients with schistosomiasis japonica.


METHODS
Liver biopsy materials were examined pathomorphologically in 26 patients with schistosomiasis in advanced stage and 5 cases without the disease as control. The expression of MMP-2, MMP-9 and C-IV were studied by immunohistochemistry, and result was analyzed by picture quantitative analysis technique.


RESULTS
Immnoreactive MMP-2 was mainly expressed in the plasma, the membrane of hepatocytes and hepatic sinusoids, it was also found in myofibroblast cells and endothelial cells of blood vessel. Immnoreactive MMP-9 was observed in hepatic stellate cells, sinusoids and myofibroblast cells, sometimes it was also seen in the plasma of hepatocytes and epithelial cells of bile duct. The expression of MMP-2, MMP-9 and C-IV was significantly stronger in patients with advanced schistosomiasis than that of the control(P < 0.05). The expression of MMP-2 was relevant to the inflammation grade and stage of liver fibrosis(P < 0.01). The expression of MMP-9 did not show significant change(P > 0.05) and the expression of C-IV was consistent with that of MMP-2.


CONCLUSIONS
The study indicates that increased expression of MMP-2 participated in the initiation and development process of liver fibrosis, MMP-9 was related to the initiation of liver fibrosis and pathological change existed in hepatic sinusoids in advanced schistosomiasis japonica.
Tao
Jun
J
Institute of Infectious Diseases, First Affiliatred Hospital, School of Medicine, Zhejiang University, Hangzhou 310003.
Cai
Wei-min
WM
Chen
Jia-lin
JL
Zhang
Yan-ping
YP
Weng
Hong-lei
HL
Liu
Rong-hua
RH
D000328:Adult; D000368:Aged; D024141:Collagen Type IV / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000201:enzymology* / Q000469:parasitology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D012554:Schistosomiasis japonica / Q000201:enzymology* / Q000378:metabolism
D024141:Collagen Type IV; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9