A study of factors affecting the labelling of tartrate with 188Re and the transchelation of the 188Re from the tartrate to a protein.
The formation of 188Re-tartrate for use in transchelation reactions and the transchelation of the 188Re onto albumin was studied. Two labelled tartrate products were separated using a non-traditional mobile phase on ITLC strips. Tartrate labelling yield increases with pH but so does the instability when the product is exposed to air. Lower pH's are preferred when oxygen-free labelling conditions can be achieved. Higher tin levels protect against air oxidation. Stability of the Re-tartrate complex is supported by addition of ascorbic acid and ferrous sulphate, however both these agents decreased the rate of the formation of the Re-tartrate complex. The labelling efficiency of a perrhenate solution decreased with the time for which it is stored prior to the labelling reaction, depending on the radioactive concentration.Re-albumin transchelation efficiency increases with the tartrate concentration, while increased stability of the Re-tartrate complex lowers the transchelation yields of Re-albumin.
Sailerova
Eva
E
Radiopharmacy, Health Sciences Centre, 820 Sherbrook Street, Winnipeg, Manitoba, Canada R3A 1R9.
Billinghurst
Mervyn W
MW
D002614:Chelating Agents / Q000737:chemistry; D004355:Drug Stability; D005079:Excipients; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D011506:Proteins / Q000737:chemistry*; D011868:Radioisotopes / Q000737:chemistry*; D012211:Rhenium / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013644:Tartrates / Q000737:chemistry*
D002614:Chelating Agents; D005079:Excipients; D011506:Proteins; D011868:Radioisotopes; D012709:Serum Albumin; D013644:Tartrates; D012211:Rhenium; C029768:tartaric acid