title: Genetic interactions among ZDS1,2, CDC37, and protein kinase CK2 in Saccharomyces cerevisiae.
abstract: We report here the identification of the homologous gene pair ZDS1,2 as multicopy suppressors of a temperature-sensitive allele (cka2-13(ts)) of the CKA2 gene encoding the alpha' catalytic subunit of protein kinase CK2. Overexpression of ZDS1,2 suppressed the temperature sensitivity, geldanamycin (GA) sensitivity, slow growth, and flocculation of multiple cka2 alleles and enhanced CK2 activity in vivo toward a known physiological substrate, Fpr3. Consistent with the existence of a recently described positive feedback loop between CK2 and Cdc37, overexpression of ZDS1,2 also suppressed the temperature sensitivity, abnormal morphology, and GA sensitivity of a CK2 phosphorylation-deficient mutant of CDC37, cdc37-S14A, as well as the GA sensitivity of a cdc37-1 allele. A likely basis for all of these effects is our observation that ZDS1,2 overexpression enhances Cdc37 protein levels. Activation of the positive feedback loop between CK2 and Cdc37 likely contributes to the pleiotropic nature of ZDS1,2, as both CK2 and Cdc37 regulate diverse cellular functions.
authors: Department of Biochemistry and Molecular Biology, Life Sciences Building, The University of Georgia, Athens, GA 30602-7229, USA.
authors: Sricharan
authors: S
authors: Bandhakavi
authors: Richard O
authors: RO
authors: McCann
authors: David E
authors: DE
authors: Hanna
authors: Claiborne V C
authors: CV
authors: Glover
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D016227:Benzoquinones; D047390:Casein Kinase II; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins; D004789:Enzyme Activation / Q000502:physiology; D016153:Genes, Suppressor; D005838:Genotype; D047029:Lactams, Macrocyclic; D008856:Microscopy, Fluorescence / Q000379:methods; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D017354:Point Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000037:antagonists & inhibitors / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D016227:Benzoquinones; C513270:CDC37 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D047029:Lactams, Macrocyclic; D018832:Molecular Chaperones; D011809:Quinones; D029701:Saccharomyces cerevisiae Proteins; C101740:ZDS1 protein, S cerevisiae; C101741:ZDS2 protein, S cerevisiae; D011505:Protein-Tyrosine Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; C001277:geldanamycin