title: Schwann cells transplantation promoted and the repair of brain stem injury in rats.
abstract: OBJECTIVE
  To explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats.
  
  
  METHODS
  Schwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively.
  
  
  RESULTS
  BrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas.
  
  
  CONCLUSIONS
  The transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.
authors: Beijing Neurosurgical Institute, Tian Tan Xili 6, Beijing 100050, China. wanhong50@hotmail.com
authors: Hong
authors: H
authors: Wan
authors: Yi-Hua
authors: YH
authors: An
authors: Mei-Zhen
authors: MZ
authors: Sun
authors: Ya-Zhuo
authors: YZ
authors: Zhang
authors: Zhong-Cheng
authors: ZC
authors: Wang
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001930:Brain Injuries / Q000628:therapy* / Q000662:veterinary; D001933:Brain Stem / Q000293:injuries*; D001973:Bromodeoxyuridine; D017690:Cell Transplantation / Q000379:methods* / Q000662:veterinary; D004594:Electrophysiology; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000637:transplantation*
chemical_list: D000963:Antimetabolites; D001973:Bromodeoxyuridine