[Investigation of p53 gene mutations in keloids using PCR-SSCP].
OBJECTIVE
To detect gene mutations of p53 gene (exon 4-6) in fibroblasts.


METHODS
Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing.


RESULTS
Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids.


CONCLUSIONS
Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.
Liu
Yong-bo
YB
Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Gao
Jian-hua
JH
Duan
Hong-jie
HJ
Liu
Xiao-jun
XJ
D017209:Apoptosis; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D005347:Fibroblasts; D016158:Genes, p53; D006801:Humans; D007627:Keloid / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D016159:Tumor Suppressor Protein p53