Chemokines and TRANCE as genetic adjuvants for a DNA vaccine to rabies virus.
An adaptive immune response is initiated by mature dendritic cells presenting processed antigen to nai;ve T cells. Assuming that the magnitude of the immune response is influenced by the number and type of antigen-presenting dendritic cells and by the duration of antigen presentation, we tested if chemokines that bind to receptors expressed on immature dendritic cells or TRANCE, a survival factor for mature dendritic cells, can serve as adjuvants. None of the immunomodulaters given as genetic adjuvants with a DNA vaccine encoding the full-length rabies virus glycoprotein augmented the transgene product-specific response. However, RANTES, MCP-1, MIP 1-beta, and TRANCE given together with a DNA vaccine expressing a truncated and thus secreted version of the rabies virus glycoprotein enhanced the response suggesting that the tested genetic adjuvants promoted preferentially presentation of reprocessed antigen originating from transduced tissue cells.
Pinto
A R
AR
The Wistar Institute, 3601 Spruce Street, Room 283, Philadelphia, PA 19104-4205, USA.
Reyes-Sandoval
A
A
Ertl
H C J
HC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002352:Carrier Proteins / Q000235:genetics*; D018925:Chemokines / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D053245:RANK Ligand; D011819:Rabies Vaccines / Q000276:immunology*; D053246:Receptor Activator of Nuclear Factor-kappa B; D019444:Vaccines, DNA / Q000276:immunology*
D000914:Antibodies, Viral; D002352:Carrier Proteins; D018925:Chemokines; D007074:Immunoglobulin G; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D053245:RANK Ligand; D011819:Rabies Vaccines; D053246:Receptor Activator of Nuclear Factor-kappa B; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D019444:Vaccines, DNA; D007371:Interferon-gamma