High-speed electrophoretic analysis of 1-phenyl-3-methyl-5-pyrazolone derivatives of monosaccharides on a quartz microchip with whole-channel UV detection.
1-Phenyl-3-methyl-5-pyrazolone (PMP) derivatives of monosaccharides were analyzed by electrophoresis on a quartz microchip with whole-channel UV detection. Rapid separation of PMP derivatives of aldopentoses was achieved by plain-zone electrophoresis in a neutral phosphate buffer with the height equivalent to a theoretical plate at the micrometer level. Zone electrophoresis as borate complexes was also successful for the separation of PMP derivatives of a few aldoses, which were separated within 1 min. Separation by microchip electrophoresis was compared to that by capillary electrophoresis, and the difference was discussed in terms of column efficiency and sample column capacity.
Suzuki
Shigeo
S
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Ishida
Yoshiro
Y
Arai
Akihiro
A
Nakanishi
Hiroaki
H
Honda
Susumu
S
D000983:Antipyrine / Q000031:analogs & derivatives*; D000077553:Edaravone; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D016166:Free Radical Scavengers; D006601:Hexoses / Q000737:chemistry / Q000302:isolation & purification; D007202:Indicators and Reagents; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010429:Pentoses / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D000516:alpha-Amylases
D016166:Free Radical Scavengers; D006601:Hexoses; D007202:Indicators and Reagents; D009005:Monosaccharides; D009844:Oligosaccharides; D010429:Pentoses; D000516:alpha-Amylases; D000077553:Edaravone; D000983:Antipyrine