Increased syndecan expression following myocardial infarction indicates a role in cardiac remodeling.
The purpose of this study was to identify essential genes involved in myocardial growth and remodeling following myocardial infarction (MI). Left ventricular noninfarcted tissues from six mice subjected to MI under general anesthesia and from six sham-operated mice were obtained 1 wk after primary surgery and analyzed by means of cDNA filter arrays. Out of a total of 1,176 genes, 641 were consistently expressed, twenty-three were upregulated and thirteen downregulated. Five genes were only expressed following MI. Syndecan-3, a transmembranous heparan sulfate proteoglycan, was found to be upregulated together with a transcriptional activator of syndecans, Wilms tumor protein 1 (WT-1). Northern blotting demonstrated a significant upregulation of syndecan-1, -2, -3, and -4, WT-1, fibronectin, and basic fibroblast growth factor (FGF) receptor 1. Furthermore, Western blot analysis showed statistically significant increases in protein levels for syndecan-3 and -4. In conclusion, we have identified a subset of genes with increased expression in noninfarcted left ventricular tissue following MI, including syndecans 1-4, WT-1, fibronectin, collagen 6A, and FGF receptor 1. Since the syndecans link the cytoskeleton to the extracellular matrix and function as required coreceptors for FGF, we suggest a role for the syndecans in cardiac remodeling following MI.
Finsen
Alexandra Vanessa
AV
Institute for Experimental Medical Research, Ullevaal University Hospital, Oslo, Norway. alexandra.finsen@ioks.uio.no
Woldbaek
Per Reidar
PR
Li
Jian
J
Wu
Jiaping
J
Lyberg
Torstein
T
Tønnessen
Theis
T
Christensen
Geir
G
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D020869:Gene Expression Profiling; D006321:Heart; D008168:Lung / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009203:Myocardial Infarction; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D011509:Proteoglycans / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D053668:Syndecan-1; D053667:Syndecans; D015854:Up-Regulation; D020257:Ventricular Remodeling / Q000235:genetics*
D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D053667:Syndecans