title: A transgenic mouse model for the detailed morphological study of astrocytes.
abstract: We have generated a transgenic mouse model in which astrocytes express an enhanced green fluorescent protein (EGFP) under the control of the mouse glial fibrillary acidic protein (GFAP) promoter. EGFP, which is characteristically found throughout the cell, was expressed in these animals even in astrocytic fine processes, and EGFP expressing cells demonstrated morphological characters of protoplasmic, fibrous, or reactive astrocytes. In contrast, GFAP immunoreactivity was found only in the perinuclear region and in the main processes. The transgenic mouse model therefore provides a valuable tool for the detailed morphological investigation of astrocytes.
authors: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors: Ryusuke
authors: R
authors: Suzuki
authors: Jun
authors: J
authors: Watanabe
authors: Satoru
authors: S
authors: Arata
authors: Hisayuki
authors: H
authors: Funahashi
authors: Sakae
authors: S
authors: Kikuyama
authors: Seiji
authors: S
authors: Shioda
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001921:Brain / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000033:anatomy & histology*; D009924:Organ Culture Techniques; D011401:Promoter Regions, Genetic
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins