Thermally reversible polymer gel for chondrocyte culture.
We have evaluated a biomaterial to serve as a scaffold for the propagation and amplification of chondrocytes that promotes the original cellular phenotype of these cells. The goal of the present study was to investigate the use of thermally reversible polymer gels poly(NiPAAm-co-AAc), as a biocompatible supporting scaffold for the propagation of chondrocytic cells. The polymer gels at temperatures above its lower critical solution temperature whereas liquefying at temperatures below its lower critical solution temperature of 34.5 degrees C. Hence, the polymer, in its gelled form, has the ability to hold cells in situ, forming a matrix similar to the natural cellular environment or the extracellular matrix that comprises cartilage. We tested the hypothesis that the polymer gel promotes cell viability and function. Human osteoblast-like cells, nasal chondrocytes, and articular chondrocytes (1 x 10(5)/150 microL) were resuspended in enriched Dulbecco's minimal essential media and were plated onto control (without gel) and gel containing 24-well plates. The plates were reincubated at 37 degrees C, 5% CO(2) for the time point of interest. Additional media was added to the plates and exchanged as needed. After cell culture, cells were retrieved, enumerated, and cell viability was determined. Other aliquots of the cells were stained for morphological analysis whereas expression of chondrocyte markers including collagen type II and aggrecan were determined using reverse transcriptase-polymerase chain reaction. The polymer gel was not cytotoxic because the cell number retrieved from three-dimensional culture gel was found to be one to two times higher than that retrieved from monolayer culture. Chondrocytes propagated in the thermo-reversible polymers expressed enhanced or maintained expression of collagen type II and aggrecan. Collagen type I expression was decreased or unaltered. The N-isopropylacrylamide and acrylic acid copolymer gel has potential use as a cell culture substrate and as a cell delivery vehicle.
Au
Angela
A
Department of Orthopaedic Surgery, Johns Hopkins University, 5601 Loch Raven Boulevard, Baltimore, Maryland 21239, USA.
Ha
Jinny
J
Polotsky
Anna
A
Krzyminski
Karol
K
Gutowska
Anna
A
Hungerford
David S
DS
Frondoza
Carmelita G
CG
D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D048429:Cell Size; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D005782:Gels / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D001672:Biocompatible Materials; D005782:Gels; D011108:Polymers