title: Molecular epidemiology of classical swine fever in the Russian Federation.
abstract: The ability to discriminate between various classical swine fever virus (CSFV) strains and isolates is a prerequisite for following the spread of the virus after an outbreak. To determine the relatedness between Russian CSFV isolates from different geographical regions, three fragments of the viral genome (5' NTR, the variable region of the E2 gene and a fragment of the NS5B gene) were sequenced and used for genetic typing. Thirty-one field isolates were obtained from CSF outbreaks which occurred between 1994 and 1999. In addition, three attenuated strains were included in the study, namely the LK and CS vaccine strains, and the moderately virulent 238H isolate. The vaccine strains have been used in Russia for more than 30 years. Our results showed that all field isolates are in subgroup 1.1 together with Alfort 187 and with the highly virulent strain Shimen. In contrast, the CS and LK vaccine strains belong to subgroup 1.2. While there is no evidence for the reversion of the two vaccine strains to wild type, it is feasible that the highly virulent Shimen strain, which has been used as a challenge strain for many years, contributed to field strain generation. The Russian field isolates from the 1990s can be distinguished from the CSF virus isolates which occurred in the EU Member States in the same decade, as here all outbreaks were caused by CSF viruses belonging to subgroup 2.
authors: NARVAC R&D Department, Ivanovski Virology Institute, Moscow, Russia.
authors: A
authors: A
authors: Vlasova
authors: T
authors: T
authors: Grebennikova
authors: A
authors: A
authors: Zaberezhny
authors: I
authors: I
authors: Greiser-Wilke
authors: G
authors: G
authors: Floegel-Niesmann
authors: V
authors: V
authors: Kurinnov
authors: T
authors: T
authors: Aliper
authors: E
authors: E
authors: Nepoklonov
mesh_terms: D000818:Animals; D001483:Base Sequence; D006691:Classical Swine Fever / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004196:Disease Outbreaks / Q000662:veterinary*; D005838:Genotype; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012426:Russia / Q000453:epidemiology; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014765:Viral Vaccines
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D014765:Viral Vaccines