title: Study on the interaction between nucleic acids and acetamiprid.
abstract: The interaction between deoxyribonucleic acid (DNA) and acetamiprid was studied. It was found that the fluorescence of acetamiprid could be enhanced in the presence of DNA in sulfuric acid solution. The excitation and emission wavelength of acetamiprid was 291 nm and 587 nm, respectively. Under optimal conditions, the calibration graph is over the range of 0.1-10 micromL(-1). The calibration limit is 0.06 microg mL(-1) (S/N = 3). The determination results of DNA in yeast cell and golden staphylococcus samples by this method were satisfactory. The mechanism of the reaction is discussed.
authors: College of Applied Chemistry, China Agricultural University, Beijing, PR China. aydy-826@163.com
authors: Nianqin
authors: N
authors: Jie
authors: Shicong
authors: S
authors: Hou
authors: Fengpei
authors: F
authors: Du
authors: Linrui
authors: L
authors: Huang
authors: Guibin
authors: G
authors: Jiang
authors: Suqin
authors: S
authors: Lv
mesh_terms: D002138:Calibration; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004271:DNA, Fungal / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D015394:Molecular Structure; D000073943:Neonicotinoids; D009691:Nucleic Acid Denaturation; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013210:Staphylococcus / Q000235:genetics; D013464:Sulfuric Acids; D013997:Time Factors; D015003:Yeasts / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D000073943:Neonicotinoids; D011725:Pyridines; D013464:Sulfuric Acids; C464485:acetamiprid; D004247:DNA; C033158:sulfuric acid