title: Auxin responsiveness of a novel cytochrome p450 in rice coleoptiles.
abstract: An early auxin-induced gene was isolated from rice (Oryza sativa L. subsp. japonica cv Nihonmasari) coleoptiles by a fluorescent-labeled differential display screen. The full-length gene contains conserved domains characteristic for the cytochrome p450 superfamily. This gene, designated as CYP87A3, was weakly expressed in dark-grown coleoptiles but was up-regulated rapidly and transiently when coleoptile segments were incubated in 5 microm indole-3-acetic acid. This induction by auxin could not be suppressed by cycloheximide. Depletion of segments from endogenous auxin reduced the amount of CYP87A3 transcripts. The CYP87A3 transcript level was rapidly, although transiently, up-regulated in response to light as well. The observed pattern of gene regulation might indicate a role in the suppression of auxin-induced coleoptile growth. The role of CYP87A3 is discussed with respect to auxin signaling in the regulation of coleoptile growth.
authors: Institut für Biologie II, Schänzlestrasse 1, D-79104 Freiburg, Germany. christian.chaban@uni-freiburg.de
authors: Christina
authors: C
authors: Chaban
authors: Frank
authors: F
authors: Waller
authors: Masaki
authors: M
authors: Furuya
authors: Peter
authors: P
authors: Nick
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology*; D010100:Oxygen / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D007210:Indoleacetic Acids; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen