DNA typing: an important step forward? Collaborative Transplant Study.
In a collaborative project which was supported by 96 transplant centers, DNA typing of HLA-DR antigens was carried out on over 7,000 transplant donors and recipients at 8 participating laboratories. Approximately 25% of the individuals were found to have been typed incorrectly by serological means. An analysis of over 2,500 first cadaver kidney transplants showed a significant correlation of matching for the HLA-DR antigens in transplants where the serological typing was confirmed by DNA typing. In transplants where the serological typing was found to be incorrect, the analysis of serological HLA-DR mismatches resulted in no correlation with graft outcome whereas a significant correlation was found when the corrected DNA typed HLA-DR antigens were analyzed. Transplants which had been reported to the Collaborative Transplant Study based on serological typing as matched for HLA-A, -B, -DR or HLA-B, -DR were found to have a superior graft survival rate only if HLA-DR compatibility was confirmed by DNA typing.
Opelz
G
G
Transplant Immunology Laboratory, University of Heidelberg, Germany.
Mytilineos
J
J
Scherer
S
S
Dunckley
H
H
Trejaut
J
J
Chapman
J
J
Middleton
D
D
Savage
D
D
Fischer
G
G
Bignon
J D
JD
Bensa
J C
JC
Albert
E
E
Noreen
H
H
D016172:DNA Fingerprinting / Q000379:methods*; D006085:Graft Survival / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D014181:Transplantation Immunology
D006684:HLA-DR Antigens