Effect of reorganized-human growth hormone on cell cycle kinetics in liver cancer in vitro.
OBJECTIVE
To study the effect of reorganized-human growth hormone (r-hGH) on cycle kinetics and apoptosis of liver cancer cells or 7402 cells.


METHODS
Liver cancer cells were cultured for 24 hours with r-hGH at different concentrations with or without cisplatin (DDP). Cells undergoing apoptosis and differentiation were determined by flow cytometry (FCM).


RESULTS
Comparison of the results in culture with and without r-hGH showed that the percentage of cells in G0-G1 phase dropped (P<0.05), whereas in S phase increased (P<0.05). Adding of r-hGH and DDP to the culture medium increased the apoptosis of liver cancer cells more significantly than adding DDP only (P<0.05).


CONCLUSIONS
Liver cancer cells might express the hGH receptor. In vitro r-hGH might induce the differentiation of liver cancer cells, stimulate the combination of DNA, and reduce the cells in G0-G1 phases. These improve the sensibility of tumors to the special-staged chemical treatment. Chemotherapy together with r-hGH may increase the apoptosis of liver cancer cells.
He
Xin-Yang
XY
Department of Surgery, Anhui Provincial Hospital, Hefei 230001, China. xugelian@mail.hf.ah.cn
Xu
Ge-Liang
GL
Xu
Rong-Nan
RN
Ze
Zhi-Min
ZM
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000378:metabolism; D004357:Drug Synergism; D005453:Fluorescence; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D019382:Human Growth Hormone; D002945:Cisplatin