Cell cycle arrest induction by an adenoviral vector expressing HIV-1 Vpr in bovine and feline cells.
An accessory protein, Vpr, of human immunodeficiency virus type 1 (HIV-1) induces the cell cycle G(2)/M arrest in primate cells, but not in rodent cells, suggesting that a species-specific factor might be involved in the phenomenon. To study whether Vpr can cause G(2)/M arrest in non-primate cells, a novel adenoviral vector, Ad-VIG, co-expressing HIV-1 Vpr and green fluorescent protein (GFP) was constructed and infected on cell lines derived from various mammalian species. With its ability to express GFP, Ad-VIG enabled flow cytometric evaluation of transduction efficiency in the infected cells, and Western blot analysis showed successful expression of Vpr in the vector-transduced cells. Upon Ad-VIG infection, human HeLa, African green monkey Vero, feline CRFK, and bovine MDBK cells manifested cell cycle G(2)/M arrest. This is the first study showing that non-primate feline and bovine cells are susceptible to Vpr-induced cell cycle arrest.
Matsuda
Mari
M
Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japan.
Matsuda
Naoto
N
Watanabe
Ayako
A
Fujisawa
Ryuichi
R
Yamamoto
Katsuhiko
K
Masuda
Michiaki
M
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D005434:Flow Cytometry; D016336:Gene Products, vpr / Q000235:genetics* / Q000502:physiology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D008957:Models, Genetic; D013045:Species Specificity; D014709:Vero Cells
D016336:Gene Products, vpr; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins