title: Small lymphocytic lymphoma, marginal zone B-cell lymphoma, and mantle cell lymphoma exhibit distinct gene-expression profiles allowing molecular diagnosis.
abstract: Non-germinal center small B-cell lymphomas represent a heterogeneous group of non-Hodgkin lymphomas, the most frequent histologic subtypes being small lymphocytic lymphoma (SLL), splenic marginal zone B-cell lymphoma (MZL), and mantle cell lymphoma (MCL). In order to identify genomic signatures specific for each disease, we analyzed 128 primary tumors using high-density microarrays. Several clusters of genes significantly discriminated the 3 histologic subtypes. Genes associated with cell adhesion, angiogenesis, and inhibition of apoptosis were up-regulated in SLL. Genes associated with intracellular signaling via the AKT1 pathway were up-regulated in splenic MZL. Genes associated with cell cycle control and multidrug resistance were up-regulated in MCL. Using 44 genes selected within the gene clusters discriminant for the 3 lymphoma subtypes, we generated a class prediction score that allowed us to classify the 3 entities in 96% of the cases, including borderline cases. Whereas specific transcriptional profiles easily distinguished all MZL samples, SLL samples, and most of the MCL samples into separate groups, few MCL cases exhibited MZL-type transcriptional profiles. This study demonstrates that SLL, splenic MZL, and MCL possess specific transcriptional profiles that may be relevant to the pathogenesis and the diagnosis of these histologic subtypes.
authors: Service d'hématologie, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France. catherine.thieblemont@chu-lyon.fr
authors: Catherine
authors: C
authors: Thieblemont
authors: Valéry
authors: V
authors: Nasser
authors: Pascale
authors: P
authors: Felman
authors: Karen
authors: K
authors: Leroy
authors: Sophie
authors: S
authors: Gazzo
authors: Evelyne
authors: E
authors: Callet-Bauchu
authors: Béatrice
authors: B
authors: Loriod
authors: Samuel
authors: S
authors: Granjeaud
authors: Philippe
authors: P
authors: Gaulard
authors: Corinne
authors: C
authors: Haioun
authors: Alexandra
authors: A
authors: Traverse-Glehen
authors: Lucile
authors: L
authors: Baseggio
authors: François
authors: F
authors: Bertucci
authors: Daniel
authors: D
authors: Birnbaum
authors: Florence
authors: F
authors: Magrangeas
authors: Stéphane
authors: S
authors: Minvielle
authors: Hervé
authors: H
authors: Avet-Loiseau
authors: Gilles
authors: G
authors: Salles
authors: Bertrand
authors: B
authors: Coiffier
authors: Françoise
authors: F
authors: Berger
authors: Rémi
authors: R
authors: Houlgatte
mesh_terms: D001706:Biopsy; D003937:Diagnosis, Differential; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D014066:Palatine Tonsil / Q000473:pathology; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D013154:Spleen / Q000473:pathology
chemical_list: D012334:RNA, Neoplasm