Desialylated N-CAM and chromatin-derived acidic peptide effects in the hypothalamus.
Chromatin-derived acidic peptides (ACPs) have been shown to acutely modulate hypothalamic catecholamine release. To investigate whether this effect is mediated through membrane polysialylated neural-cell adhesion molecule (PSA-N-CAM), we pretreated rat hypothalamic synaptosomes with neuraminidase enzyme, which partially cleaves sialic acid residues from N-CAM, and perfused them with ACP-1 (Asp-Asp-Ser-Asp-Glu-Glu-Asn) or a more lipophilic derivative, ACP-2 ([Ala-Ile-Ser-Pro]-Asp-Asp-Ser-Asp-Glu-Glu-Asn). We have found that neuraminidase completely abolish the inhibitory effect of ACP-1 on dopamine release, while the inhibitory activity of ACP-1 on norepinephrine release is partially lost. On the other hand, ACP-2 inhibition of dopamine release is not modified by neuraminidase pretreatment.
Nisio
Chiara Di
CD
Department of Drug Sciences, School of Pharmacy, G. D'Annunzio University, via dei Vestini, 66013, Chieti, Italy.
Brunetti
Luigi
L
Esposito
Diana L
DL
Recinella
Lucia
L
Orlando
Giustino
G
Michelotto
Barbara
B
Vacca
Michele
M
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism
D002843:Chromatin; D019006:Neural Cell Adhesion Molecules; D010455:Peptides; D009439:Neuraminidase