Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells.
We investigated whether zinc deficiency can affect plasma membrane rheology. Three cell lines, human leukaemia T-cells (Jurkat), rat fibroblasts (3T3) and human neuroblastoma cells (IMR-32), were cultured for 48 h in control medium, in zinc-deficient medium (1.5 microM zinc; 1.5 Zn), or in the zinc-deficient medium supplemented with 15 microM zinc (15 Zn). The number of viable cells was lower in the 1.5 Zn group than in the control and 15 Zn groups. The frequency of apoptosis was higher in the 1.5 Zn group than in the control and 15 Zn groups. Membrane fluidity was evaluated using the 6-(9-anthroyloxy)stearic acid and 16-(9-anthroyloxy)palmitic acid probes. Membrane fluidity was higher in 1.5 Zn cells than in the control cells; no differences were observed between control cells and 15 Zn cells. The effect of zinc deficiency on membrane fluidity at the water/lipid interface was associated with a higher phosphatidylserine externalization. The higher membrane fluidity in the hydrophobic region of the bilayer was correlated with a lower content of arachidonic acid. We suggest that the increased fluidity of the membrane secondary to zinc deficiency is in part due to a decrease in arachidonic acid content and the apoptosis-related changes in phosphatidylserine distribution.
Verstraeten
Sandra V
SV
Department of Biological Chemistry, IQUIFIB (UBA-CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
Zago
M Paola
MP
MacKenzie
Gerardo G
GG
Keen
Carl L
CL
Oteiza
Patricia I
PI
D016475:3T3 Cells; D000634:Aminoquinolines / Q000737:chemistry; D000818:Animals; D017209:Apoptosis; D002462:Cell Membrane / Q000737:chemistry*; D002470:Cell Survival; D005227:Fatty Acids / Q000032:analysis; D005456:Fluorescent Dyes; D006801:Humans; D019169:Jurkat Cells; D008560:Membrane Fluidity; D051379:Mice; D010718:Phosphatidylserines / Q000032:analysis; D014105:Tosyl Compounds / Q000737:chemistry; D015032:Zinc / Q000032:analysis / Q000172:deficiency / Q000502:physiology*
D000634:Aminoquinolines; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010718:Phosphatidylserines; D014105:Tosyl Compounds; C061730:N-(6-methoxy-8-quinolyl)-4-toluenesulfonamide; D015032:Zinc