[Effect of coriaria lactone on the ATP-sensitive potassium channels in pyrimidal neurons of rats].
OBJECTIVE
To acquire knowledge about the effect of Coriaria lactone (CL) on ATP-sensitive potassium channels (KATP) in cultured pyrimidal neurons of rats and the role of KATP in epileptogenesis.


METHODS
Experiments were conducted by the use of cell culture technique and single channel patch clamp technique.


RESULTS
The reversion potential of neuron KATP was nearly 0 mV under the condition of the same high K+ level in both bath solution and pole solution. The channel could be blocked by TEA, and the channel current could be induced by 30 mumol/L DNP but inhibited by 0.5 mol/L ATP. The conductance of KATP channel current was 78.23 +/- 12.04 pS, and this KATP channel could be remarkably activated by CL and specifically blocked by Glibenclamide.


CONCLUSIONS
The activation of neuron KATP may play a role of decreasing the action potential and thus protecting the neurons from the seizure induced by CL.
Zou
Xiaoyi
X
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Hua
H
Zhou
Shushun
S
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D004827:Epilepsy / Q000209:etiology; D005260:Female; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000166:cytology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D007783:Lactones; D015221:Potassium Channels; C049836:coriaria lactone; D000255:Adenosine Triphosphate