Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition.
The mechanisms regulating mitochondrial outer-membrane permeabilization and the release of cytochrome c during apoptosis remain controversial. In the present study, we show in an in vitro model system that the release of cytochrome c may occur via moderate modulation of mitochondrial volume, irrespective of the mechanism leading to the mitochondrial swelling. In contrast with mitochondrial permeability transition-dependent release of cytochrome c, in the present study mitochondria remain intact and functionally active.
Gogvadze
Vladimir
V
Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Box 210, SE-171 77 Stockholm, Sweden.
Robertson
John D
JD
Enoksson
Mari
M
Zhivotovsky
Boris
B
Orrenius
Sten
S
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism*; D007473:Ion Channels / Q000502:physiology*; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009997:Osmotic Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D014634:Valinomycin / Q000494:pharmacology
D007473:Ion Channels; D007476:Ionophores; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D014634:Valinomycin; D045304:Cytochromes c; D002118:Calcium