[Effects of As2O3 on tissue factor, plasminogen activator inhibitor-1 and -2 expression in NB4, HL-60 and THP-1 cells].
OBJECTIVE
To explore the effects of arsenic trioxide (As2O3) on tissue factor (TF), plasminogen activator inhibitor-1(PAI-I) and PAI-2 expression in NB4, HL-60 and THP-1 cells.


METHODS
Inhibition of the cell proliferation of NB4, HL-60 and THP-1 cells treated with As2O3 in different concentrations was observed by use of growth curves. Apoptosis was assessed by DNA electrophoresis and flow cytometric DNA contents analysis. TF, PAI-1 and PAI-2 antigen levels in cell lysates were measured by enzyme-linked immunoassay (ELISA).


RESULTS
1. After 24 hours exposure to 1 mumol/L As2O3 or 8 mumol/L As2O3, induced apoptosis of NB4 or HL-60 cells was noted. However induced apoptosis of THP-1 cells was not seen after exposure to 8 mumol/L As2O3 for 24 h. 2. The TF antigen levels were down-regulated during the As2O3-induced apoptosis of NB4 cells (P < 0.01), and the down-regulation effect of TF antigen levels was in the As2O3-concentration dependent fashion. Moreover, 2 mumol/L As2O3 increased the PAI-1 antigen levels in NB4 cells (P < 0.05). 3. The TF antigen levels were up-regulated and the PAI-2 antigen levels were down-regulated during the As2O3-induced apoptosis of HL-60 cells (P < 0.01 and P < 0.05, TF and PAI-2 respectively). 4. The TF and PAI-2 antigen levels in THP-1 cells were up-regulated after the exposure to 8 mumol/L As2O3 for 24 h (P < 0.05 and P < 0.01, TF and PAI-2 respectively).


CONCLUSIONS
The effects of As2O3 on TF, PAI-1 and PAI-2 antigen levels in NB4, HL-60 and THP-1 cells are distinct.
Xu
Xia
X
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Peng
Liming
L
D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology; D010087:Oxides / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D017396:Plasminogen Activator Inhibitor 2 / Q000096:biosynthesis* / Q000235:genetics; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D001152:Arsenicals; D010087:Oxides; D017395:Plasminogen Activator Inhibitor 1; D017396:Plasminogen Activator Inhibitor 2; D013925:Thromboplastin; D000077237:Arsenic Trioxide