title: A peptide switch regulates DNA polymerase processivity.
abstract: Chromosomal DNA polymerases are tethered to DNA by a circular sliding clamp for high processivity. However, lagging strand synthesis requires the polymerase to rapidly dissociate on finishing each Okazaki fragment. The Escherichia coli replicase contains a subunit (tau) that promotes separation of polymerase from its clamp on finishing DNA segments. This report reveals the mechanism of this process. We find that tau binds the C-terminal residues of the DNA polymerase. Surprisingly, this same C-terminal "tail" of the polymerase interacts with the beta clamp, and tau competes with beta for this sequence. Moreover, tau acts as a DNA sensor. On binding primed DNA, tau releases the polymerase tail, allowing polymerase to bind beta for processive synthesis. But on sensing the DNA is complete (duplex), tau sequesters the polymerase tail from beta, disengaging polymerase from DNA. Therefore, DNA sensing by tau switches the polymerase peptide tail on and off the clamp and coordinates the dynamic turnover of polymerase during lagging strand synthesis.
authors: Howard Hughes Medical Institute and Laboratory of DNA Replication, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.
authors: Francisco J
authors: FJ
authors: López de Saro
authors: Roxana E
authors: RE
authors: Georgescu
authors: Mike
authors: M
authors: O'Donnell
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004258:DNA Polymerase III / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors
chemical_list: C022262:Okazaki fragments; D010455:Peptides; D011994:Recombinant Proteins; D004247:DNA; C023140:DNA replicase; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D003545:Cysteine