Biosensor for rapid phosphate monitoring in a sequencing batch reactor (SBR) system.
A thick-film phosphate biosensor based on hydrogel immobilized pyruvate oxidase (POD) has been developed for rapid phosphate process control monitoring in an experimental sequencing batch reactor (SBR) system. We have employed a phosphate biosensor in an off-line monitoring of phosphate concentrations in a bench scale SBR. Measurements with biosensor show a good correlation (r2=0.98) with those of commercial colorimetric phosphate testing kits. The signal response time was 1 min with a detection limit of 5 microM. The biosensor method showed a good operational stability, needed less experimental procedures and a small sample size (approximately 20 microl). This allows its practical application for rapid phosphate measurements to obtain real time process data in a SBR system.
Mak
Wing Cheung
WC
Sino-German Nano-Analytical Lab (SiGNAL), Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. chmartin@ust.hk
Chan
Chiyui
C
Barford
John
J
Renneberg
Reinhard
R
D019149:Bioreactors; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D009862:Online Systems; D010710:Phosphates / Q000032:analysis* / Q000737:chemistry*; D011771:Pyruvate Oxidase / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004800:Enzymes, Immobilized; D010710:Phosphates; D014874:Water Pollutants, Chemical; D011771:Pyruvate Oxidase