title: Determination of genetic sex in chinook salmon (Oncorhynchus tshawytscha) using the male-linked growth hormone pseudogene by real-time PCR.
abstract: This study used a real-time quantitative polymerase chain reaction (qPCR) method based on the growth hormone pseudogene (GHp) in chinook salmon (Oncorhynchus tshawytscha) to determine genetic sex. The GHp is present as a single copy in the genome of the male chinook salmon but is absent in the female, providing a means of using this real-time qPCR method to discriminate genetic sex. Comparisons between genomic DNA samples from 2 geographically distinct populations of chinook salmon (Columbia River, Washington, and Yukon River, Alaska) showed, within each population examined, that the males were clearly differentiated from the females. There were no interpopulation differences between males or females. The advantages of this real-time qPCR method are that it is rapid, is amenable to high sample throughput, and provides an accurate numerical value that allows comparisons between samples by statistical methods.
authors: Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3051, USA, jamesn@uidaho.edu.
authors: James J
authors: JJ
authors: Nagler
authors: Tim
authors: T
authors: Cavileer
authors: Kirk
authors: K
authors: Steinhorst
authors: Robert H
authors: RH
authors: Devlin
mesh_terms: D000413:Alaska; D000818:Animals; D017931:DNA Primers; D005456:Fluorescent Dyes; D013006:Growth Hormone / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011544:Pseudogenes / Q000235:genetics*; D012474:Salmon / Q000235:genetics*; D012732:Sex Determination Analysis / Q000379:methods*; D014861:Washington
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D013006:Growth Hormone