Choice of procedures for preparative chromatography.
Quantitative criteria, necessary and sufficient, were developed basing on the analysis of asymtotic solutions of border equations in elution sorption dynamics for realization of selectivity inversion for components in a chromatographic system due to the effect of kinetic selectivity. Analysis is suggested for experiments, where the new approach to realization of chromatographic processes using effect of kinetic selectivity allows optimization of preparative separation of biologically active substances. The approach suggested implies shortening of the experiment duration for separation processes, which can be crucial in most systems where components to be isolated are labile, or the process economics suffers considerably due to mobile phase, or energy consumption.
Pisarev
Oleg A
OA
Department of Biopolymers, Institute of Macromolecular Compounds, Russian Academy of Science, Bolshoi pr. 31, St. Petersburg 199004, Russia. pisarev@imc.macro.ru
Glasova
Natalia V
NV
D002853:Chromatography, Liquid / Q000379:methods*; D007700:Kinetics; D012680:Sensitivity and Specificity