2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced activation of mitogen-activated protein kinase signaling pathway in Jurkat T cells.
The present study was performed to examine mitogen-activated protein kinase associated pathways in mediation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced cell apoptosis in cultured Jurkat T cells. TCDD significantly decreased cell viability in a concentration-dependent manner (P<0.05 at 10-300 nM). TCDD (10 nM) also time-dependently decreased cell viability (P<0.05 at 12-48 hr). c-Jun NH2-terminal kinase was significantly phosphorylated with TCDD treatment in a time dependent manner. p38 Mitogen-activated protein kinase was not significantly changed with TCDD treatment. Extracellular signal-regulated protein kinase was significantly phosphorylated with TCDD treatment for 8 hr and gradually returned to baseline. TCDD induced up-regulation of ASK1 and C-Jun, which are up- and down-stream of JNK, respectively, and up-regulation of cytosolic cytochrome c and caspase-3. These results demonstrate that MAPK signaling pathways including JNK and ERK 1/2, are activated with the treatment of TCDD in Jurkat T cells, which suggest that MAPK pathways may be involved in TCDD-induced cell death.
Kwon
Myung-Ja
MJ
Medicinal Science Division, Korea Research Institute of Chemical Technology, Daejon, Korea.
Jeong
Kyu-Shik
KS
Choi
Eun Jeong
EJ
Lee
Byung Ho
BH
D002470:Cell Survival / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D004789:Enzyme Activation; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D019169:Jurkat Cells; D048670:MAP Kinase Kinase 4; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism* / Q000502:physiology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases
D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D020929:Mitogen-Activated Protein Kinase Kinases