Identification of a new HLA-DRB5 allele, DRB5*0112, by routine PCR-SSP.
We describe the identification of a new HLA-DRB5 allele found in two members of a British Caucasoid family. Genomic analysis of exon 2 revealed a novel sequence. The name DRB5*0112 has been assigned to this allele by the WHO Nomenclature Committee, and the EMBL sequence database accession number for this is AJ427352. DRB5*0112 has several unique sequence features when compared to other DRB5 alleles, and comparison with all known DRB1/3/4/5 alleles indicates this to have arisen as a result of intraexonic recombination between a DRB5-containing haplotype and one carrying DRB1*09.
Atkinson
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DC
Histocompatibility and Immunogenetics Laboratory, National Blood Service, Vincent Drive, Edgbaston, Birmingham, UK. david.atkinson@nbs.nhs.uk
Jobson
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SE
Dunn
P P J
PP
Briggs
D C
DC
D000483:Alleles; D000595:Amino Acid Sequence; D005091:Exons; D006684:HLA-DR Antigens / Q000235:genetics*; D059847:HLA-DRB5 Chains; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D006684:HLA-DR Antigens; D059847:HLA-DRB5 Chains