[Experimental research on human spermatozoa membrane proteins by two-dimensional gel electrophoresis].
OBJECTIVE
To analyze human spermatozoa membrane proteins by two-dimensional gel electrophoresis and to provide a basis for drawing the protein map of normal human spermatozoa membrane proteins.


METHODS
Spermatozoa were purified by Percoll density centrifugation, and spermatozoa membrane proteins were analyzed by two-dimensional gel electrophoresis using isoelectric focusing and polyacrylamide gel electrophoresis.


RESULTS
About 800 protein spots could be identified by the imaging analysis system. The molecular weight and isoelectric point of most proteins were 20,000 to 100,000 and 3.0 to 7.0 respectively.


CONCLUSIONS
There are more than 800 types of proteins in the human spermatozoa membrane. The spermatozoa membrane protein can be identified with a certain precision by two-dimensional gel electrophoresis.
Wang
Hao-Fei
HF
Department of Urology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. wangblack@citiz.net
Xiang
Zu-Qiong
ZQ
Wang
Yi-Xin
YX
D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry*; D008970:Molecular Weight; D013094:Spermatozoa / Q000737:chemistry*
D008565:Membrane Proteins