title: Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization.
abstract: Metabolomic characteristics in boar spermatozoa were studied using [1,2-(13)C(2)]glucose and mass isotopomer analysis. In boar spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis was seen. Slight glycogen synthesis through the direct pathway and some incorporation of pyruvate into the Krebs cycle also took place. Neither RNA ribose-5-phosphate nor fatty acid synthesis from glucose occurred despite the detection of pyruvate dehydrogenase activity. In contrast to the known metabolic activities in dog sperm, boar spermatozoa have low levels of energy production and biosynthetic activities suggesting two different metabolic profiles for the two different phenotypes.
authors: Department of Biochemistry and Molecular Biology, University of Barcelona, C/ Martí i Franqués 1, Barcelona 08028, Spain.
authors: Silvia
authors: S
authors: Marin
authors: Kelly
authors: K
authors: Chiang
authors: Sara
authors: S
authors: Bassilian
authors: Wai-Nang Paul
authors: WN
authors: Lee
authors: Laszlo G
authors: LG
authors: Boros
authors: Josep Maria
authors: JM
authors: Fernández-Novell
authors: Josep Joan
authors: JJ
authors: Centelles
authors: Antonio
authors: A
authors: Medrano
authors: Joan Enric
authors: JE
authors: Rodriguez-Gil
authors: Marta
authors: M
authors: Cascante
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002952:Citric Acid Cycle / Q000502:physiology; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis; D007773:Lactates / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010427:Pentose Phosphate Pathway / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D005227:Fatty Acids; D007773:Lactates; D006003:Glycogen; D005947:Glucose