title: Expression of cyclooxygenase-1 and -2 in human breast cancer.
abstract: OBJECTIVE
  We investigated the expression of cyclooxygenase (Cox-1 and Cox-2) in mammary tissues from patients with breast cancer.
  
  
  METHODS
  We used reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry.
  
  
  RESULTS
  The cancer cells showed very weak expression of Cox-1, but strong expression of immunoreactive Cox-2. In contrast, immunoreactive Cox-2 was very weak in all of the benign mammary tumors examined, including fibroadenoma (FA) and mastopathy (MP). Immunoreactive Cox-1 was also very weak in these benign tumors. The extent and intensity of immunoreactive Cox-2 polypeptides was significantly greater in the cancer cells than in the FA cells or MP cells. RT-PCR analysis showed enhanced expression of Cox-2, but not Cox-1 in breast cancer tissue, and faint expression of Cox-2 in benign tissue.
  
  
  CONCLUSIONS
  These results demonstrated that human breast cancer cells generated Cox-2, indicating that Cox-2 might play an important role in the proliferation of breast cancer cells.
authors: Department of Organ Transplant and Endocrinological Surgery, Kyoto Prefectural University of Medicine, 465 Hirokoji, Kawaramachi, Kamigyo-ku, 602-0841 Kyoto, Japan.
authors: Norio
authors: N
authors: Yoshimura
authors: Hajime
authors: H
authors: Sano
authors: Masahiko
authors: M
authors: Okamoto
authors: Kiyokazu
authors: K
authors: Akioka
authors: Hidetaka
authors: H
authors: Ushigome
authors: Yayoi
authors: Y
authors: Kadotani
authors: Rikio
authors: R
authors: Yoshimura
authors: Shuji
authors: S
authors: Nobori
authors: Atsushi
authors: A
authors: Higuchi
authors: Yoshihiro
authors: Y
authors: Ohmori
authors: Kenji
authors: K
authors: Nakamura
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000473:pathology*; D015331:Cohort Studies; D046508:Culture Techniques; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D008565:Membrane Proteins; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; D008565:Membrane Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases