Lysozyme-lysozyme and lysozyme-salt interactions in the aqueous saline solution: a new square-well potential.
We investigate lysozyme-lysozyme and lysozyme-salt interactions in electrolyte solutions using a molecular-thermodynamic model. An equation of state based on the statistical mechanical perturbation theory is applied to describe the interactions. The perturbation term includes a new square-well potential of mean force, which implies the information about the lysozyme surface and salt type. The attractive energy of the potential of mean force is correlated with experimental cloud-point temperatures of lysozyme in various solution conditions. The same attractive energy is used to predict osmotic pressure of a given system with no additional parameters. The new potential shows a satisfactory improvement in understanding the interactions between lysozymes in aqueous salt solutions.
Chang
Bong Ho
BH
Division of Chemical Engineering and Molecular Thermodynamics Lab., Hanyang University, Seoul 133-791, Korea.
Bae
Young Chan
YC
D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008962:Models, Theoretical; D009113:Muramidase / Q000737:chemistry*; D009997:Osmotic Pressure; D011506:Proteins / Q000737:chemistry; D012492:Salts / Q000737:chemistry*; D012996:Solutions
D011506:Proteins; D012492:Salts; D012996:Solutions; D009113:Muramidase