title: Mechanisms of platelet retention in the collagen-coated-bead column.
abstract: Although the glass-bead column has been used to measure platelet adhesion, whether platelet interaction with glass beads represents physiologic processes remains unsettled. In an attempt to obtain more physiologic platelet responses, plastic beads coated with type I collagen have been recently developed to replace glass beads. In this study, we analyzed the factors responsible for platelet retention in the collagen-coated-bead column and investigated its possible clinical applications. We pumped citrated whole-blood samples into columns at a fixed speed with an injection pump and calculated platelet-retention rates by measuring platelet counts before and after passage through the columns. The platelet-retention rates, which were highly reproducible with samples from healthy donors, were reduced in a patient with glycoprotein (GP) VI deficiency but not in patients with type III von Willebrand disease. Anti-GPIIb/IIIa antibody and GRGDS peptide markedly inhibited platelet retention, whereas inhibition of the GPIb-von Willebrand factor or GPIa/IIa-collagen interaction had no effect. Data on the effects of various antiplatelet agents (including the antithrombin agent argatroban, prostacyclin, acetylsalicylic acid, and the ADP scavenger creatine phosphate/creatine phosphokinase) support the usefulness of this assay method in clinical application. Our findings suggest that GPVI and GPIIb/IIIa but not the GPIb-von Willebrand factor interaction are mainly involved in platelet retention in this column.
authors: Department of Clinical and Laboratory Medicine, Yamanashi Medical University, Nakakoma, Yamanashi, Japan.
authors: Makoto
authors: M
authors: Kaneko
authors: Olga
authors: O
authors: Cuyun-Lira
authors: Toshiro
authors: T
authors: Takafuta
authors: Katsue
authors: K
authors: Suzuki-Inoue
authors: Kaneo
authors: K
authors: Satoh
authors: Kayoko
authors: K
authors: Ohtsuki
authors: Masatoshi
authors: M
authors: Ohnishi
authors: Morio
authors: M
authors: Arai
authors: Yutaka
authors: Y
authors: Yatomi
authors: Yukio
authors: Y
authors: Ozaki
mesh_terms: D000244:Adenosine Diphosphate / Q000502:physiology; D003094:Collagen / Q000494:pharmacology*; D006801:Humans; D010973:Platelet Adhesiveness; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000502:physiology; D040201:Platelet Membrane Glycoprotein IIb / Q000502:physiology; D010980:Platelet Membrane Glycoproteins / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000502:physiology; D014841:von Willebrand Factor / Q000502:physiology
chemical_list: D010975:Platelet Aggregation Inhibitors; D019038:Platelet Glycoprotein GPIb-IX Complex; D040201:Platelet Membrane Glycoprotein IIb; D010980:Platelet Membrane Glycoproteins; D018048:Receptors, Purinergic P2; C098341:platelet membrane glycoprotein VI; D014841:von Willebrand Factor; D000244:Adenosine Diphosphate; D003094:Collagen