title: Functional, structural, and distribution analysis of the chorionic gonadotropin receptor using murine monoclonal antibodies.
abstract: LH and human chorionic gonadotropin (hCG) control steroid production and gametogenesis. They also function as growth factors through interaction with a specific receptor that is a member of the seven-transmembrane receptor family coupled via G proteins to signal pathways involving cAMP and phospholipase C/inositol 3 phosphate. For this study, monoclonal antibodies (mAbs) were raised against the human LH receptor (LHR)/hCG receptor (hCGR), using Chinese hamster ovary LHR-transfected cells as the immunogen. Two reagents were then selected on the basis of their ability to recognize the full-length transmembrane receptor expressed both by Chinese hamster ovary LHR-transfected cells and by a limited number of tumor cell lines. One of these mAbs reacts with the LHR/hCGR in tissue sections of both frozen and paraffin-embedded specimens. This unique feature allowed us to map the cytological distribution of LHR/hCGR in human breast tissues at different stages of development in physiological and benign pathological conditions. The same mAb proved to be agonistic: receptor ligation elicits signals that modulate the growth of selected breast tumor cell lines. This observation suggests that the mAb recognizes an epitope that is included in the domain of the receptor involved in the interaction with the natural ligand.
authors: Department of Genetics, Biology and Biochemistry and Research Center for Experimental Medicine, University of Torino, Torino 10126, Italy.
authors: Ada
authors: A
authors: Funaro
authors: Anna
authors: A
authors: Sapino
authors: Bruna
authors: B
authors: Ferranti
authors: Alberto L
authors: AL
authors: Horenstein
authors: Isabella
authors: I
authors: Castellano
authors: Bruno
authors: B
authors: Bagni
authors: Gianni
authors: G
authors: Garotta
authors: Fabio
authors: F
authors: Malavasi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D001940:Breast / Q000737:chemistry / Q000378:metabolism; D016466:CHO Cells; D002455:Cell Division / Q000276:immunology; D045744:Cell Line, Tumor; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D018604:Epitope Mapping; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006825:Hybridomas; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D016612:Paraffin Embedding; D011974:Receptors, LH / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D005404:Fixatives; D011974:Receptors, LH; D005557:Formaldehyde; D000242:Cyclic AMP