Specific antibodies induced by nasally administered 40-kDa outer membrane protein of Porphyromonas gingivalis inhibits coaggregation activity of P. gingivalis.
In this study, we have assessed the efficacy of the 40-kDa outer membrane protein (40k-OMP) of Porphyromonas gingivalis as a nasal vaccine for the prevention of adult periodontitis. Mice nasally immunized with 40k-OMP and cholera toxin as mucosal adjuvant displayed significant levels of 40k-OMP-specific serum IgG1, IgG2b and IgA as well as mucosal IgA antibodies (Abs) in saliva and nasal secretions. Ab-forming cell (AFC) analysis confirmed the antibody titers by detecting high numbers of 40k-OMP-specific AFCs in spleen, salivary glands and nasal passages. Because 40k-OMP-specific IgG inhibited coaggregation of P. gingivalis vesicles and S. gordonii, it may be an important tool for the prevention of adult periodontitis.
Namikoshi
Jun
J
Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, Matsudo, 271-8587, Chiba, Japan.
Otake
Shigeo
S
Maeba
Satomi
S
Hayakawa
Mitsuo
M
Abiko
Yoshimitsu
Y
Yamamoto
Masafumi
M
D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage* / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016966:Porphyromonas gingivalis / Q000276:immunology*; D012463:Saliva / Q000276:immunology / Q000382:microbiology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G