Characterization of genetic differences between Mycobacterium avium subsp. paratuberculosis type I and type II isolates.
A combination of representational difference analysis and comparative DNA sequencing revealed that four type I (sheep) isolates of Mycobacterium avium subsp. paratuberculosis were differentiated from nine type II (bovine) isolates by the presence of an 11-bp insertion in a novel M. avium subsp. paratuberculosis-specific region of genomic DNA. Further, our studies show that M. avium subsp. paratuberculosis type I isolates contain three type-specific loci that are missing in M. avium subsp. paratuberculosis type II but are present in M. avium subsp. avium. Taken together, the results are consistent with the hypothesis that M. avium subsp. paratuberculosis type I strains are an evolutionary intermediate between M. avium subsp. avium and M. avium subsp. paratuberculosis type II isolates or share a subset of M. avium subsp. avium type-specific loci through horizontal transfer.
Dohmann
Karen
K
Department of Infectious Diseases, Institute for Microbiology, School of Veterinary Medicine Hannover, D-30173 Hannover, Germany.
Strommenger
Birgit
B
Stevenson
Karen
K
de Juan
Lucía
L
Stratmann
Janin
J
Kapur
Vivek
V
Bull
Tim J
TJ
Gerlach
Gerald-Friedrich
GF
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial