A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions.
We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate. In addition to elevated throughput, the PicoTiterPlate based amplifications (PTPCR) can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 microL reaction volume for the entire PicoTiterPlate. The bulk PTPCR product can be recovered and assayed with real-time PCR, or discrete PTPCR products can be driven to solid supports, enabling downstream applications such as translation/transcription or sequencing.
Leamon
John H
JH
454 Life Sciences, Branford, CT 06405, USA. jleamon@454.com
Lee
William L
WL
Tartaro
Karrie R
KR
Lanza
Janna R
JR
Sarkis
Gary J
GJ
deWinter
Alex D
AD
Berka
Jan
J
Weiner
Michael
M
Rothberg
Jonathan M
JM
Lohman
Kenton L
KL
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers; D005336:Fiber Optic Technology; D008855:Microscopy, Electron, Scanning; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004247:DNA