Efficacy of DNA-hsp65 vaccination for tuberculosis varies with method of DNA introduction in vivo.
A DNA vaccine codifying the mycobacterial hsp65 can prevent infection with Mycobacterium tuberculosis in a prophylactic setting and also therapeutically reduce the number of bacteria in infected mice. The protective mechanism is thought to be related to Th1-mediated events that result in bacterial killing. To determine the best method of hsp65 introduction for vaccination efficacy against tuberculosis (TB), we evaluated the immunogenicity and protection of DNA-hsp65 administered by gene gun bombardment or intramuscular (i.m.) injection of naked DNA. Immunization by gene gun induced immune response with plasmid doses 100-fold lower than those required for intramuscular immunization. However, in contrast to intramuscular immunization, which was protective in these studies, gene gun immunization did not protect BALB/c mice against challenge infection.
Lima
K M
KM
Instituto do Milênio REDE-TB, Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.
dos Santos
S A
SA
Santos
R R
RR
Brandão
I T
IT
Rodrigues
J M
JM
Silva
C L
CL
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000276:immunology*; D019470:Biolistics; D018834:Chaperonin 60; D018833:Chaperonins / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D006046:Gold; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D032581:Tuberculosis Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016207:Cytokines; D004269:DNA, Bacterial; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; C074119:heat-shock protein 65, Mycobacterium; D006046:Gold; D018833:Chaperonins