title: Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC.
abstract: An HPLC method for the determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf has been developed. The separation system consisted of a C18 reversed-phase column, a gradient elution system of methanol/water and orthophosphoric acid, and a photodiode array detector. The concentrations of hamamelitannin, gallic acid, (+)-gallocatechin and (+)-catechin were 4.77, 0.59, 0.22 and 0.39% (w/w), respectively, in the bark. Hamamelitannin and catechins were also detected in the leaves at concentration less than 0.04% (w/w). This method is simple, sensitive and reproducible, ideally suited for rapid, routine analysis.
authors: R&D Department, William Ransom & Son plc, Hitchin, Herts SG5 1LY, UK. hwang@williamransom.com
authors: Huafu
authors: H
authors: Wang
authors: Gordon J
authors: GJ
authors: Provan
authors: Keith
authors: K
authors: Helliwell
mesh_terms: D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D029501:Hamamelis; D006601:Hexoses / Q000032:analysis*; D024301:Plant Bark; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves; D018547:Plant Stems
chemical_list: D006601:Hexoses; D010936:Plant Extracts; D005707:Gallic Acid; D002392:Catechin; C093412:hamamelitannin