Poly(styrene/alpha-tert-butoxy-omega-vinylbenzylpolyglycidol) microspheres for immunodiagnostics. Principle of a novel latex test based on combined electrophoretic mobility and particle aggregation measurements.
The principle of a novel latex agglutination test based on combined results of electrophoretic mobility and particle aggregation measurements is described. Poly(styrene/alpha-tert-butoxy-omega-vinylbenzylpolyglycidol) (P(S/PGL)) microspheres were synthesized by a one step soap-free emulsion copolymerization of styrene and alpha-tert-butoxy-omega-vinylbenzylpolyglycidol macromonomer with number average molecular weight Mn = 2700 (polydispersity [Mw]/[Mn] = 1.10). Particles with monomodal size distribution (number average diameter Dn = 220 nm) and surface fraction of polyglycidol equal to f = 0.42 mol % were obtained. Human serum albumin (HSA) was covalently bound onto the surface of P(S/PGL) microspheres activated with 1,3,5-trichlorotriazine. In a model immunodiagnostic assay for anti-HSA, in which P(S/PGL) particles with covalently bound HSA have been used, the electrophoretic mobility and aggregation of microspheres were measured simultaneously. This approach allowed detection of anti-HSA in the serum in the range of anti-HSA concentrations from 0.1 to 150 microg/mL. The highest changes in electrophoretic mobility were registered for microspheres with surface concentration of immobilized HSA equal to Gamma = 9.2 x 10(-4) g/m2.
Radomska-Galant
Izabela
I
Department of Biotechnology and Food Science, Institute of Technical Biochemistry Technical University of Lodz, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland.
Basinska
Teresa
T
D000327:Adsorption; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis; D006801:Humans; D007159:Immunologic Tests / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008863:Microspheres; D011137:Polystyrenes; D020005:Propanols / Q000737:chemistry; D012709:Serum Albumin / Q000276:immunology; D014753:Vinyl Compounds / Q000737:chemistry
D000906:Antibodies; D011137:Polystyrenes; D020005:Propanols; D012709:Serum Albumin; D014753:Vinyl Compounds