title: A soluble molecule secreted by human blastocysts modulates regulation of HOXA10 expression in an epithelial endometrial cell line.
abstract: OBJECTIVE
  To determine the existence of a soluble signal, secreted from the human blastocyst embryo, that induces HOXA10 gene expression before cell-cell contact.
  
  
  METHODS
  To analyze, by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), cell-free media that had contained human embryos cultured to the blastocyst stage for a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa).
  
  
  METHODS
  Assisted reproduction technology program of Yale University, New Haven, Connecticut.
  
  
  METHODS
  Patients undergoing intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) cycles. Treatment of Ishikawa cells with blastocyst-conditioned media.
  
  
  METHODS
  Determination of HOXA10 gene expression.
  
  
  RESULTS
  We demonstrate that cell-free media that had contained human embryos cultured to the blastocyst stage contain a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa). We found that hCG does not induce HOXA10 in Ishikawa cells.
  
  
  CONCLUSIONS
  Soluble molecules induce a well-characterized marker of endometrial receptivity in endometrial cells without blastocyst apposition. Additionally, HOXA10 induction can serve as a means of evaluating human embryos cultured for IVF and ET. High quality embryos may induce local endometrial receptivity before trophectoderm-endometrial contact.
authors: Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut 06520, USA.
authors: Denny
authors: D
authors: Sakkas
authors: Christina
authors: C
authors: Lu
authors: Ebru
authors: E
authors: Zulfikaroglu
authors: Evelyn
authors: E
authors: Neuber
authors: Hugh S
authors: HS
authors: Taylor
mesh_terms: D001685:Biological Factors / Q000737:chemistry / Q000502:physiology*; D001755:Blastocyst / Q000378:metabolism*; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000494:pharmacology / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005314:Embryonic and Fetal Development / Q000502:physiology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012995:Solubility; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D001685:Biological Factors; D006063:Chorionic Gonadotropin; D017077:Culture Media, Conditioned; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C092119:Hoxa10 protein, mouse