T-cell receptor Vbeta gene usage by lymphocytes infiltrating human renal allografts.
T cell lines have been derived from human kidney allograft biopsies using mitogenic stimulation. Southern blotting using a T-cell receptor (TCR) Cbeta probe revealed an oligoclonal pattern of rearranged bands in all 12 samples analysed. In some cases, differences in band patterns were noted between independent cultures from the same biopsy. Most T-cell clones derived from 2 biopsies showed different patterns of rearranged bands. The polymerase chain reaction (PCR) was used to study TCR Vbeta gene usage in allograft-derived T-cell cultures. This was more sensitive and more informative than Southern blotting and revealed that most TCR Vbeta genes were expressed in T cells from biopsies showing cellular rejection. The potential usefulness of this technique to quantify TCR V gene usage in allospecific T-cell populations is discussed.
Gecim
I E
IE
Renal Transplant Unit, Royal Liverpool University Hospital, Liverpool, United Kingdom.
Christmas
S E
SE
Brew
R
R
Flanagan
B F
BF
Wheatcroft
N J
NJ
Bakran
A
A
Sells
R A
RA
D004247:DNA / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D004247:DNA