Synaptic loss following depletion of noradrenaline and/or serotonin in the rat visual cortex: a quantitative electron microscopic study.
Biogenic amines have a trophic-like role for the formation and the maintenance of synapses in the CNS. We examined the changes in the number of synaptic profiles in the developing and adult rat visual cortex following selective depletion of noradrenaline and/or serotonin. By the drug-induced decreases in levels of noradrenaline or serotonin between 1 and 2 weeks after birth, the number of synaptic profiles was decreased by 29-55% compared with that of control animals. The magnitude of reduction in the number of synaptic profiles was virtually the same following simultaneous depletion of both noradrenaline and serotonin compared with the depletion of noradrenaline or serotonin alone. Later in the developmental period, the function of noradrenaline and serotonin in facilitating synapse formation and maintenance became less prominent than that in younger animals. In the control animals, the number of axosomatic synapses was the highest at around 2 weeks after birth, and decreased with development. The number of axodendritic synapses was the highest between 2 and 7 weeks after birth, and decreased to 50% at 11 weeks after birth. These data demonstrate that synapses in the rat visual cortex are overproduced during the early developmental period. We suggest that both serotonin and noradrenaline are necessary for synapse formation during the early stages of development of the rat visual cortex.
Matsukawa
M
M
Department of Anatomy, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba, Ibaraki 305-8577, Japan. matukawa@med.nihon-u.ac.jp
Nakadate
K
K
Ishihara
I
I
Okado
N
N
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001596:Benzylamines / Q000633:toxicity; D002452:Cell Count; D004347:Drug Interactions; D010134:Fenclonine / Q000633:toxicity; D008297:Male; D008854:Microscopy, Electron; D009473:Neuronal Plasticity / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D014179:Neurotransmitter Uptake Inhibitors / Q000633:toxicity; D009638:Norepinephrine / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000633:toxicity; D013569:Synapses / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014793:Visual Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D001596:Benzylamines; D014179:Neurotransmitter Uptake Inhibitors; D012702:Serotonin Antagonists; D012701:Serotonin; C012102:DSP 4; D010134:Fenclonine; D009638:Norepinephrine