title: Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, gram stain-negative blood cultures.
abstract: Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.
authors: Bacteriology Laboratory, University of Basel Hospitals and Clinics, 4031 Basel, Switzerland. hadler@uhbs.ch
authors: Hildegard
authors: H
authors: Adler
authors: Nathalie
authors: N
authors: Baumlin
authors: Reno
authors: R
authors: Frei
mesh_terms: D000165:Acridine Orange; D001769:Blood / Q000382:microbiology; D005189:False Positive Reactions; D005456:Fluorescent Dyes; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange