title: Eyes absent represents a class of protein tyrosine phosphatases.
abstract: The Eyes absent proteins are members of a conserved regulatory network implicated in the development of the eye, muscle, kidney and ear. Mutations in the Eyes absent genes have been associated with several congenital disorders including the multi-organ disease bronchio-oto-renal syndrome, congenital cataracts and late-onset deafness. On the basis of previous analyses it has been shown that Eyes absent is a nuclear transcription factor, acting through interaction with homeodomain-containing Sine oculis (also known as Six) proteins. Here we show that Eyes absent is also a protein tyrosine phosphatase. It does not resemble the classical tyrosine phosphatases that use cysteine as a nucleophile and proceed by means of a thiol-phosphate intermediate. Rather, Eyes absent is the prototype for a class of protein tyrosine phosphatases that use a nucleophilic aspartic acid in a metal-dependent reaction. Furthermore, the phosphatase activity of Eyes absent contributes to its ability to induce eye formation in Drosophila.
authors: Division of Developmental Biology, Cincinnati Childrens Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA.
authors: Jayanagendra P
authors: JP
authors: Rayapureddi
authors: Chandramohan
authors: C
authors: Kattamuri
authors: Brian D
authors: BD
authors: Steinmetz
authors: Benjamin J
authors: BJ
authors: Frankfort
authors: Edwin J
authors: EJ
authors: Ostrin
authors: Graeme
authors: G
authors: Mardon
authors: Rashmi S
authors: RS
authors: Hegde
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology* / Q000235:genetics; D005123:Eye / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D005136:Eye Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D029721:Drosophila Proteins; D005136:Eye Proteins; C092694:eya protein, Drosophila; D017027:Protein Tyrosine Phosphatases