Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells.
A cell colony (IM95m) that produces hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and interleukin-8 (IL-8) was cloned from gastric cancer cells (IM95 cell line). In culture medium, the highest levels of HGF, VEGF, and IL-8 were about 1.1, 0.9, and 0.17 ng/ml culture medium at 3 d from 10(5) cells. IM95m may be useful in elucidating the role of tumor cells in angiogenesis.
Iwai
Mineko
M
Department of Applied Bio-Science, Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka 599-8570, Japan. iwaimi@riast.osakafu-u.ac.jp
Matsuda
Masahiko
M
Iwai
Yoshiaki
Y
D002455:Cell Division; D045744:Cell Line, Tumor / Q000378:metabolism*; D048429:Cell Size; D019976:Cloning, Organism; D003470:Culture Media / Q000737:chemistry; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D003470:Culture Media; D016209:Interleukin-8; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor