[The regulating effect of antisense-S-Oligo on TYR gene expression and melanin production of melanocytes].
OBJECTIVE
Despite the causes for melanin increase, the increased gene expression of TYR is a common pathological process. Based on this viewpoint, antisense-S-Oligo of TYR was designed and synthesized to regulate synthesis of melanin in order to explore the treatment for skin pigmentation.


METHODS
The cultured melanocytes were divided into 3 groups. The group 1 was treated with endothelin, group 2 treated with ultraviolet ray and group 3 was used as the control. In each group, the 5' antisense-S-Oligo, the 3' antisense-S-Oligo, the mixed antisense-S-Oligo of TYR or Dotap only was added. The melanin content and TYR gene expressions were examined.


RESULTS
The 5' antisense-S-Oligo, the 3' antisense-S-Oligo and the mixed antisense-S-Oligo significantly inhibited the increase of melanin content and TYR gene expression, which were caused by endothelin or ultraviolet ray treatment. Of the three treatments, the 3' antisense-S-Oligo showed the strongest effect.


CONCLUSIONS
Antisense-S-Oligo has significant regulating effects on TYR gene expression and melanin content. The 3' antisense-S-Oligo is more effective than the 5' antisense-S-Oligo.
Zhang
Yu-guang
YG
Department of Plastic and Reconstructive Surgery of Shanghai the Ninth People's Hospital, Shanghai 200011, China.
Hu
Qiong-hua
QH
Wang
Xun-zhang
XZ
Qi
Zuo-liang
ZL
Lin
Xiao-xi
XX
Fang
Jian-lin
JL
Dai
Chuan-chang
CC
D024509:3' Flanking Region / Q000235:genetics; D024506:5' Flanking Region / Q000235:genetics; D016232:Endothelins / Q000494:pharmacology; D015870:Gene Expression; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D014443:Tyrosine / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays
D016232:Endothelins; D008543:Melanins; D020319:Oligodeoxyribonucleotides, Antisense; D014443:Tyrosine