title: Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
abstract: We have conducted a human cDNA project to predict protein-coding sequences (CDSs) in large cDNAs (> 4 kb) since 1994, and the number of newly identified genes, known as KIAA genes, already exceeds 2000. The ultimate goal of this project is to clarify the physiological functions of the proteins encoded by KIAA genes. To this end, the project has recently been expanded to include isolation and characterization of mouse KIAA-counterpart genes. We herein present the entire sequences and the chromosome loci of 500 mKIAA cDNA clones and 13 novel cDNA clones that were incidentally identified during this project. The average size of the 513 cDNA sequences reached 4.3 kb and that of the deduced amino acid sequences from these cDNAs was 816 amino acid residues. By comparison of the predicted CDSs between mouse and human KIAAs, 12 mKIAA cDNA clones were assumed to be differently spliced isoforms of the human cDNA clones. The comparison of mouse and human sequences also revealed that four pairs of human KIAA cDNAs are derived from single genes. Notably, a homology search against the public database indicated that 4 out of 13 novel cDNA clones were homologous to the disease-related genes.
authors: Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
authors: Noriko
authors: N
authors: Okazaki
authors: Reiko
authors: R
authors: Kikuno
authors: Reiko
authors: R
authors: Ohara
authors: Susumu
authors: S
authors: Inamoto
authors: Haruhiko
authors: H
authors: Koseki
authors: Shuichi
authors: S
authors: Hiraoka
authors: Yumiko
authors: Y
authors: Saga
authors: Takahiro
authors: T
authors: Nagase
authors: Osamu
authors: O
authors: Ohara
authors: Hisashi
authors: H
authors: Koga
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary / Q000235:genetics*; D030541:Databases, Genetic; D019204:GTP-Binding Proteins; D051379:Mice; D009419:Nerve Tissue Proteins; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C491491:Rims2 protein, mouse; D019204:GTP-Binding Proteins