Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice.
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by: (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.
Ryu
H I
HI
Bios Limited, 314 Basic Science Institute, Yeungnam University, Kyungsan, Korea.
Kim
C J
CJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D005591:Chemical Fractionation; D006968:Hypersensitivity, Delayed; D007106:Immune Sera / Q000276:immunology; D007111:Immunity, Cellular; D007116:Immunization, Passive; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D010326:Pasteurella Infections / Q000276:immunology / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000737:chemistry* / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000254:growth & development / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; D008070:Lipopolysaccharides