Significance of expression of heat shock protein90alpha in human gastric cancer.
OBJECTIVE
To evaluate the significance of hsp90alpha expression in human gastric cancer tissues.


METHODS
Immunohistochemical staining was used in clinical specimens from 33 cases of gastric cancer and 33 cases of gastritis with rabbit anti-human hsp90alpha multi-clonal antibody in order to explore the relationship between the expression of hsp90alpha in gastric carcinoma tissue and gastritis tissue as well as in mucous membrane adjacent to cancer and lymph node metastasis.


RESULTS
Hsp90alpha was detected in 88% of gastric carcinoma cases and 55% of gastritis cases. The hsp90alpha positive rate in gastric cancer group was significantly higher than that in gastritis group (P<0.01, P=0.005). The hsp90alpha positive rate in gastric cancer and in mucous membrane adjacent to cancer was 88% and 55% respectively (P<0.01, P=0.005). The hsp90alpha positive rate in lymph node metastasis group and non-lymph node metastasis group was 100% and 60% respectively, and a significant correlation between hsp90alpha expression and lymph node metastasis was shown (P<0.01, P=0.005).


CONCLUSIONS
The hsp90alpha expression rate in gastric cancer group was significantly higher than that in gastritis group as well as that in the group of mucous membrane adjacent to cancer. The hsp90alpha expression in lymphatic node metastasis group was higher than that in non-lymphatic node metastasis group. The results indicate that increased hsp90alpha expression has a close relationship with occurrence and lymph node metastasis of gastric cancer.
Zuo
Dong-Sheng
DS
Department of Pathology, Zhangjiakou Medical College, Zhangjiakou 075029, Hebei Province, China. dshz2003@yahoo.com.cn
Dai
Jie
J
Bo
Ai-Hua
AH
Fan
Jie
J
Xiao
Xiu-Ying
XY
D014408:Biomarkers, Tumor; D005756:Gastritis / Q000378:metabolism / Q000473:pathology; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000556:secondary*
D014408:Biomarkers, Tumor; D018841:HSP90 Heat-Shock Proteins