title: The effect of hydrogen peroxide applied to the middle ear on inner ear function.
abstract: OBJECTIVE
  The objective was to assess the effect of hydrogen peroxide applied to the middle ear on cochlear and vestibular function.
  
  
  METHODS
  Prospective animal study.
  
  
  METHODS
  Sand rats underwent a right-side total labyrinthectomy, and a polyethylene tube was inserted into the left-side middle ear. Following baseline recordings of vestibular evoked potentials in response to linear acceleration stimuli and auditory brainstem response, each experimental animal received five daily applications of hydrogen peroxide into the left-side middle ear. Two control groups received saline and gentamicin, respectively. Subsequently, recordings were repeated and compared with baseline measurements.
  
  
  RESULTS
  Saline administration affected neither vestibular evoked potentials nor auditory brainstem response. In contrast, both responses could not be recorded following gentamicin application. After hydrogen peroxide administration, auditory brainstem response could not be recorded in 25% (3 of 12) of the animals, whereas in the remaining nine animals the average auditory brainstem response threshold was significantly elevated by 55 dB (P =.000002). Linear vestibular evoked potentials could not be recorded in 42% (5 of 12) of the animals.
  
  
  CONCLUSIONS
  It appears that topical hydrogen peroxide adversely affects both cochlear and vestibular function of the sand rat. The study demonstrated the effect of a reactive oxygen species on inner ear function and may be useful in the study of mechanisms responsible for this damage and its protection. Clinically, although an animal model was used in the present study, caution should be exercised when large amounts of hydrogen peroxide are applied to a dry, perforated ear.
authors: Department of Otolaryngology-Head and Neck Surgery, Shaare Zedek Medical Center and Faculty of Health Sciences, Ben-Gurion University of the Negev, Jerusalem, Israel.
authors: Ronen
authors: R
authors: Perez
authors: Sharon
authors: S
authors: Freeman
authors: David
authors: D
authors: Cohen
authors: Jean-Yves
authors: JY
authors: Sichel
authors: Haim
authors: H
authors: Sohmer
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000009:adverse effects*; D001309:Auditory Threshold / Q000187:drug effects; D003051:Cochlea / Q000187:drug effects; D007758:Ear, Inner / Q000601:surgery*; D004432:Ear, Middle / Q000601:surgery*; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects*; D005849:Gerbillinae; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000009:adverse effects*; D013506:Otologic Surgical Procedures; D011446:Prospective Studies; D051381:Rats; D017382:Reactive Oxygen Species; D012965:Sodium Chloride / Q000008:administration & dosage; D014722:Vestibule, Labyrinth / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D006861:Hydrogen Peroxide