[Effects of vacuum-assisted closure (VAC) on the expressions of MMP-1, 2, 13 in human granulation wound].
OBJECTIVE
To study the changes of metalloproteinases 1, 2, 13 in granulation wound after the treatment of vacuum-assisted closure (VAC).


METHODS
The chronic wounds in 5 patients were treated with VAC. The expressions of MMP-1, 2, 13 in the granulation tissues of the chronic wounds were determined and quantified using RT-PCR technique before and at 1, 4, 7 days after the treatment.


RESULTS
The MMP-1, 13 mRNA showed obvious decrease, with the steepest variation of MMP-13. The MMP-2 mRNA also showed a decreased tendency, though in an undulatory fashion.


CONCLUSIONS
VAC can promote healing of chronic wounds through depressing the expressions of MMP-1, 2, 13 mRNA and protein synthesis, depressing the degradations of collagen and gelatin.
Shi
Bing
B
Department of Plastic and Burn, Tang-Du Hospital, Fourth Military Medical University, Xi'an 710038, China.
Chen
Shao-zong
SZ
Zhang
Ping
P
Li
Jin-qing
JQ
D003094:Collagen / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D054843:Negative-Pressure Wound Therapy; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014945:Wound Healing
D003094:Collagen; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1