Phenotypic and functional analysis of bovine gammadelta lymphocytes.
The studies have provided the first comprehensive comparison of the factors regulating activation and proliferation of WC1+ and WC1- gammadelta T cells. The investigation has shown that accessory molecules essential for activation and function of WC1+ and WC1- gammadelta T cells and the sources and roles of cytokines in activation of gammadelta T cells through the T cell receptor (TCR). The study has also shown that the role of cytokines in activation and function of gammadelta T cells activated indirectly through cytokines secreted by ab T cells, accessory cells and antigen presenting cells (APC). Cytokines were differentially produced by subpopulations of gammadelta T cells under different conditions of activation. The investigation obtained in this study has revealed that factors account for activation and proliferation of gammadelta T cells in cultures designed to study MHC-restricted responses to antigens. Evidence obtained here has shown there is biological relevance to activation under these culture conditions that points to potential regulatory and effector functions of gammadelta T cells. The investigations have also provided the information needed to begin identifying and characterizing antigens recognized by the TCR repertoires of WC1+ and WC1- gammadelta T cells. Finally, the investigations have provided the information needed to begin analysis of the mechanisms by which gammadelta T cells modulate MHC restricted immune responses to pathogens and derived vaccines.
Park
Y H
YH
Department of Microbiology and Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea. yhp@plaza.snu.ac.kr
Yoo
H S
HS
Yoon
J W
JW
Yang
S J
SJ
An
J S
JS
Davis
W C
WC
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003208:Concanavalin A; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D016207:Cytokines; D017931:DNA Primers; D016692:Receptors, Antigen, T-Cell, gamma-delta; D003208:Concanavalin A