Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids.
Combination of capillary electrophoresis with mass spectrometry (CE-MS) allows generation of polypeptide patterns of body fluids. In a single CE-MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (M(r) > 10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected. Comparison to the normal urine pattern yielded a surprisingly low consensus: a number of polypeptides present in urine were missing. We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers.
Kaiser
Thorsten
T
Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.
Hermann
Alena
A
Kielstein
Jan T
JT
Wittke
Stefan
S
Bartel
Sebastian
S
Krebs
Ronald
R
Hausadel
Frank
F
Hillmann
Meike
M
Golovko
Igor
I
Koester
Peer
P
Haller
Hermann
H
Weissinger
Eva M
EM
Fliser
Danilo
D
Mischak
Harald
H
D003956:Dialysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010455:Peptides