title: Inhibiting effects of Achyranthes bidentata polysaccharide and Lycium barbarum polysaccharide on nonenzyme glycation in D-galactose induced mouse aging model.
abstract: OBJECTIVE
  To investigate the inhibiting effects and mechanism of achyranthes bidentata polysaccharide (ABP) and lycium barbarum polysaccharide (LBP) on nonenzyme glycation in D-galactose induced mouse aging model.
  
  
  METHODS
  Serum AGE levels were determined by AGE-ELISA, MTT method was used to determine lymphocyte proliferation, IL-2 activity was determined by a bioassay method. Spontaneous motor activity was used to detect mouse's neuromuscular movement, latency of step-through method was used to examine learning and memory abilities of mouse, colormetric assay was used to determine hydroxyproline concentration in mouse skin, pyrogallol autoxidation method was used to determine superoxide dismutase (SOD) activity of erythrocytes.
  
  
  RESULTS
  Decreased levels of serum AGE, hydroxyproline concentration in mouse skin and spontaneous motor activity in D-galactose mouse aging model were detected after treated with ABP or LBP, while lymphocyte proliferation and IL-2 activity, learning and memory abilities, SOD activity of erythrocytes, were enhanced.
  
  
  CONCLUSIONS
  ABP and LBP could inhibit nonenzyme glycation in D-galactose induced mouse aging model in vivo and ABP has a better inhibiting effect than LBP.
authors: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
authors: Hong-Bin
authors: HB
authors: Deng
authors: Da-Peng
authors: DP
authors: Cui
authors: Jian-Ming
authors: JM
authors: Jiang
authors: Yan-Chun
authors: YC
authors: Feng
authors: Nian-Sheng
authors: NS
authors: Cai
authors: Dian-Dong
authors: DD
authors: Li
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000375:Aging / Q000502:physiology*; D000818:Animals; D004195:Disease Models, Animal; D004912:Erythrocytes; D005260:Female; D005690:Galactose / Q000737:chemistry*; D007858:Learning; D032305:Lycium / Q000737:chemistry*; D008568:Memory; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D011134:Polysaccharides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D011134:Polysaccharides; D013482:Superoxide Dismutase; D005690:Galactose