title: Differential expression of a cutaneous corticotropin-releasing hormone system.
abstract: We completed the mapping of a cutaneous CRH signaling system in two species with widely different determinants of skin functions, humans and mice. In human skin, the CRH receptor (CRH-R) 1 was expressed in all major cellular populations of epidermis, dermis, and subcutis with CRH-R1alpha being the most prevalent isoform. The CRH-R2 gene was expressed solely in hair follicle keratinocytes and papilla fibroblasts, whereas CRH-R2 antigen was localized predominantly in hair follicles, sebaceous and eccrine glands, muscle and blood vessels. In mouse skin, the CRH-R2 gene and protein were widely expressed in all cutaneous compartments and in cultured normal and malignant melanocytes. CRH-binding protein mRNA was present in dermal fibroblasts, melanoma cells, and sc fat of human skin and undetectable in mouse skin. The urocortin II gene was expressed equally in mouse and human skin. Taken together with our previous investigations, the present studies document the preferential expression of CRH-R1 in human skin, which mirrors CRH-R2 expression patterns in human and mouse skin. They are likely reflecting different functional activities of human and mouse skin. The adnexal location of CRH-R2 suggests a role for the receptor in hair growth. The differential interspecies CRH signaling expression pattern probably reflects adaptation to species-specific skin function determinants.
authors: Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, 930 Madison Avenue, 5th Floor, Memphis, TN 38163, USA. aslominski@utmem.edu
authors: Andrzej
authors: A
authors: Slominski
authors: Alexander
authors: A
authors: Pisarchik
authors: Desmond J
authors: DJ
authors: Tobin
authors: Joseph E
authors: JE
authors: Mazurkiewicz
authors: Jacobo
authors: J
authors: Wortsman
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D020405:Dermis / Q000737:chemistry; D004817:Epidermis / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000737:chemistry; D015870:Gene Expression; D018859:Hair Follicle / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000737:chemistry; D008544:Melanocytes / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D018019:Receptors, Corticotropin-Releasing Hormone / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012535:Scalp; D015398:Signal Transduction; D012867:Skin / Q000737:chemistry*; D013045:Species Specificity; D054832:Urocortins
chemical_list: D002352:Carrier Proteins; D020033:Protein Isoforms; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; C518358:urocortin 2, mouse; C067133:corticotropin releasing factor-binding protein; D003346:Corticotropin-Releasing Hormone