title: Human metapneumovirus infection in Thai children.
abstract: Human metapneumovirus (hMPV) associated with clinical respiratory tract infection (RTI) in children was first isolated in The Netherlands. Of 120 Thai paediatric patients with RTI examined, 5 cases (4.2%) showed detectable hMPV based on N-gene-specific RT-PCR. All of them were negative for hRSV infection. Aligning the sequences with a reference strain revealed some nucleotide differences, which necessitates future investigation to evaluate clinical significance and genotype variation.
authors: Viral Hepatitis Research Unit, Department of Paediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors: Wanida
authors: W
authors: Thanasugarn
authors: Rujipat
authors: R
authors: Samransamruajkit
authors: Pijitra
authors: P
authors: Vanapongtipagorn
authors: Nuanchan
authors: N
authors: Prapphal
authors: Bernadette
authors: B
authors: Van den Hoogen
authors: Albert D M E
authors: AD
authors: Osterhaus
authors: Yong
authors: Y
authors: Poovorawan
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007223:Infant; D008297:Male; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D016415:Sequence Alignment; D013785:Thailand / Q000453:epidemiology
chemical_list: D012367:RNA, Viral