[Study on the effect of H2O2 against Acanthamoeba in vitro].
OBJECTIVE
To detect the effect of H2O2 on Acanthamoeba spp..


METHODS
By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested.


RESULTS
At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites.


CONCLUSIONS
H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.
Zhao
Qun-fei
QF
State Key Laboratory of Bio-Orgnic and Natural Product Chemistry, Shanghai Institute of Orgnic Chemistry, Chinese Academy of Science, Shanghai 200032.
Gao
Xue-liang
XL
Qian
Min
M
D000048:Acanthamoeba / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D015823:Acanthamoeba Keratitis / Q000469:parasitology; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D021261:Parasitic Sensitivity Tests
D000891:Anti-Infective Agents, Local; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase