Characterisation of the intact rainbow trout vitellogenin protein and analysis of its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation time-of-flight-mass spectrometry and electrospray ionisation quadrupole/time-of-flight mass spectrometry.
Vitellogenin (VTG) is a protein produced by the liver of oviparous animals. It is being used as a biomarker for exposure to endocrine disruptors in many species. Rainbow trout Vtg has recently been sequenced by the conventional cDNA nucleotide approach. We focused on protein characterization of the intact protein and its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation and electrospray ionisation mass spectrometry. The molecular mass of the intact protein was found to be 183127 Da. A large number of unidentified peptide ions encourage further structural analysis to propose possible sequence variants and post-translational modifications.
Banoub
Joseph
J
Science, Oceans and Environment Branch, Fisheries and Oceans Canada, St John's, PO Box 5667, Newfoundland A1C 5X1, Canada. banoubjo@dfo-mpo.gc.ca
Thibault
Pierre
P
Mansour
Atef
A
Cohen
Alejandro
A
Heeley
David H
DH
Jackman
Donna
D
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000737:chemistry*; D014819:Vitellogenins / Q000032:analysis / Q000737:chemistry*
D010446:Peptide Fragments; D014819:Vitellogenins; D014357:Trypsin; D003488:Cyanogen Bromide