Increased thymocyte CD4+CD8+ cells and T-cell receptor beta gene rearrangements in thymoma.
Epithelial cells in thymoma and thymic carcinoma may influence T-cell development in the tumor. In this study, we investigated the cell surface phenotype and T-cell receptor (TCR) gene rearrangement of thymocytes in thymic tumors. TCR rearrangement was observed in all cases of thymoma (A, AB, B1-2). A faint band in each digestion suggested the deletion between D1 to C1 or D1 to J2, and an additional rearrangement band with BamHI suggested the rearrangement between D1 to J1. High percentages of CD1+ cells and CD4+CD8+ (DP) cells were detected in all cases of thymoma (A, AB, B1-2). There are two kinds of cell surface phenotypes increased in populations of thymoma; one is increased DP cells and the other is a relatively low percentage of DP cells accompanied by a relatively high percentage of CD3+CD69+ cells. These findings suggest that thymocytes in thymoma are derived from immature T-cell expansion.
Sakuraba
Motoki
M
Department of Surgery I, Tokyo Women's Medical University School of Medicine, 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan.
Motoji
Toshiko
T
Nitta
Sumio
S
Mizoguchi
Hideaki
H
Ohnuki
Takamasa
T
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D005260:Female; D005434:Flow Cytometry; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D013945:Thymoma / Q000276:immunology*; D013953:Thymus Neoplasms / Q000276:immunology*