title: Microsatellite variability and heterozygote deficiency in the arctic-alpine Alaskan wheatgrass (Elymus alaskanus) complex.
abstract: Genetic variation in the allotetraploid grass Elymus alaskanus complex was assessed using microsatellites in seven populations from Canada, Greenland, and the U.S.A. Microsatellite variation was compared with allozyme and RAPD variation. Our results indicated that E. alaskanus was highly homozygous but also highly variable. The polymorphic loci ranged from 50 to 100% with a mean of 78.6%, and the mean number of allele per locus was 3.14. Average expected heterozygosity value (HE, gene diversity) varied across populations and ranged from 0.244 to 0.651 with mean of 0.414. The mean value of HE across Canadian populations (0.517) was significantly higher than that across populations in Greenland (0.367). The correlation between allozyme and microsatellite gene diversity value (HE) showed a high positive correlation (r = 0.68), but between RAPD and microsatellite showed a low positive correlation (r = 0.08). Populations were highly differentiated, with 38% of variation among populations. Interpopulation genetic distance showed no association with geographic distance between the population sites of origin. A Hardy-Weinberg exact test for all loci and all populations reveals a significant heterozygote deficiency. Possible explanations for heterozygote deficiency are discussed.
authors: Biology Department, Saint Mary's University, Halifax, Canada. genlou.sun@smu.ca
authors: Genlou
authors: G
authors: Sun
authors: Björn
authors: B
authors: Salomon
mesh_terms: D002170:Canada; D031749:Elymus / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006116:Greenland; D006579:Heterozygote; D007178:Inbreeding; D007527:Isoenzymes / Q000235:genetics; D016013:Likelihood Functions; D018895:Microsatellite Repeats / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D014481:United States
chemical_list: D007527:Isoenzymes