The influence of antimicrobial treatments on the cytocompatibility of polyurethane biosensor membranes.
The cytocompatibility of polyurethane membranes was tested following ultraviolet or gamma irradiation as well as treatment with hydrogen peroxide or glutaraldehyde containing solutions. Despite the fact that all of the methods had been recommended for antimicrobial treatment of glucose biosensors, the treatments investigated significantly influenced cytocompatibility characteristics. Cytotoxicity of membrane eluates was not observed following irradiation treatments. This was also the case when the membranes were repeatedly washed following chemical treatment. Cell growth upon the membranes was stimulated to a different extent after gamma and UV irradiation as well as following hydrogen peroxide treatments. Residues of an urea-based hydrogen peroxide inclusion compound caused a restriction in cell growth upon the membranes as was similarly observed with 2 and 4% glutaraldehyde solutions acting over 2 and 4 h, respectively. It is concluded that cytocompatibility in vitro reflecting the host response against a biomaterial in vivo does not only depend upon the material itself but also upon antimicrobial treatments which could have consequences for its bioperformance characteristics.
von Woedtke
Th
T
Institute of Pharmacy, The Ernst Moritz Arndt University of Greifswald, D-17487 Greifswald, Germany. woedtke@mail.uni-greifswald.de
Schlosser
M
M
Urban
G
G
Hartmann
V
V
Jülich
W-D
WD
Abel
P U
PU
Wilhelm
L
L
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001672:Biocompatible Materials / Q000009:adverse effects; D015374:Biosensing Techniques; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D005347:Fibroblasts / Q000473:pathology*; D005720:Gamma Rays; D005947:Glucose / Q000032:analysis*; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D011140:Polyurethanes / Q000737:chemistry* / Q000528:radiation effects*; D019736:Prostheses and Implants / Q000009:adverse effects; D013242:Sterilization / Q000379:methods*; D013499:Surface Properties; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011140:Polyurethanes; D006861:Hydrogen Peroxide; D005947:Glucose; D005976:Glutaral