title: Prognostic relevance of mitotic activity in patients with node-negative breast cancer.
abstract: The prognostic relevance of mitotic activity was analyzed in a series of 306 patients with node-negative breast cancer treated with locoregional therapy alone, until early relapse. Mitotic activity was evaluated as the number of mitotic figures per 10 high-power fields (mitotic activity index) or per 1000 tumor cells (mitotic index). Counting was carried out blindly by two observers. A high correlation was observed between the two determinations (r(s) =.96, P <.001). For clinical analysis, three mitotic activity index subgroups (mitotic figures/field </= 9, 10-19 and more than 19, according to grading criteria) and three mitotic index subgroups (percentage of mitotic figures less than 0.10, 0.11-0.50 and more than 0.50, according to tertile criteria) were considered. No relation was observed between mitotic variables and 6-year disease-free survival, whereas distant disease-free survival was strongly related to mitotic figures per 10 fields (85%, 89% and 70%, P =.012) and to the percentage of mitotic figures out of a total 1000 tumor cells (87%, 86% and 75%, P =.017). Similarly, both mitotic indices were significantly related to 6-year overall survival (99%, 95% and 77%, P <.001, for mitotic figures per 10 fields and 99%, 93% and 82%, P <.001, for the percentage of mitotic figures). These findings were particularly evident in patients with tumors of 1-2 cm. In conclusion, a high number of mitotic figures is associated with a higher probability of developing distant metastases and a shorter survival. The critical point remains the standardization of the preanalytical and analytical steps within quality control programs.
authors: Pathology Unit, Division of Oncology and Diagnostics, Pierantoni Hospital, Forlì, Italy.
authors: Laura
authors: L
authors: Medri
authors: Annalisa
authors: A
authors: Volpi
authors: Oriana
authors: O
authors: Nanni
authors: Anna Maria
authors: AM
authors: Vecci
authors: Annita
authors: A
authors: Mangia
authors: Francesco
authors: F
authors: Schittulli
authors: Franco
authors: F
authors: Padovani
authors: Donata Casadei
authors: DC
authors: Giunchi
authors: Alfredo
authors: A
authors: Zito
authors: Alfredo
authors: A
authors: Vito
authors: Dino
authors: D
authors: Amadori
authors: Angelo
authors: A
authors: Paradiso
authors: Rosella
authors: R
authors: Silvestrini
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006801:Humans; D016013:Likelihood Functions; D008198:Lymph Nodes / Q000473:pathology*; D008875:Middle Aged; D008940:Mitotic Index; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D015203:Reproducibility of Results; D016019:Survival Analysis