Association of inflammation markers with impaired insulin sensitivity and coagulative activation in obese healthy women.
Insulin resistance is associated with a low chronic inflammatory state. In this study we investigated the relationship between impaired insulin sensitivity and selected markers of inflammation and thrombin generation in obese healthy women. We examined 32 healthy obese women (body mass index > or = 28), with normal insulin sensitivity (NIS, n = 14) or impaired insulin sensitivity (n = 18), and 10 nonobese women (body mass index < 25). Impaired insulin sensitivity patients had significantly higher levels of C-reactive protein (CRP), TGF-beta 1, plasminogen activator inhibitor-1 (PAI-1), activated factor VII (VIIa), and prothrombin fragment 1 + 2 (F1 + 2) compared with either control subjects or NIS patients. On the other hand, NIS patients had higher CRP, TGF-beta 1, PAI-1, and factor VIIa, but not F1 + 2, levels than controls. Significant inverse correlations were observed between the insulin sensitivity index and TGF-beta 1, CRP, PAI-1, factor VIIa, and F1 + 2 levels. Moreover, significant direct correlations were noted between TGF-beta 1 and CRP, PAI-1, factor VIIa, and F1 + 2 concentrations. Finally, multiple regressions revealed that TGF-beta 1 and the insulin sensitivity index were independently related to F1 + 2. Our results are the first to document an in vivo relationship between insulin sensitivity and coagulative activation in obesity. The elevated TGF-beta 1 levels detected in the obese population may provide a biochemical link between insulin resistance and an increased risk for cardiovascular disease.
Romano
Mario
M
Department of Medicine and Aging, University of Chieti G. D'Annunzio, School of Medicine, 66013 Chieti, Italy.
Guagnano
Maria Teresa
MT
Pacini
Giovanni
G
Vigneri
Sergio
S
Falco
Angela
A
Marinopiccoli
Marina
M
Manigrasso
Maria Rosaria
MR
Basili
Stefania
S
Davì
Giovanni
G
D000328:Adult; D015415:Biomarkers; D001777:Blood Coagulation / Q000276:immunology*; D002097:C-Reactive Protein / Q000378:metabolism; D015942:Factor VIIa / Q000378:metabolism; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000276:immunology*; D008875:Middle Aged; D009765:Obesity / Q000276:immunology*; D010446:Peptide Fragments / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D011498:Protein Precursors / Q000097:blood; D011516:Prothrombin; D013917:Thrombin / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood
D015415:Biomarkers; D010446:Peptide Fragments; D017395:Plasminogen Activator Inhibitor 1; D011498:Protein Precursors; D016212:Transforming Growth Factor beta; C029733:prothrombin fragment 1; C044183:prothrombin fragment 2; D011516:Prothrombin; D002097:C-Reactive Protein; D015942:Factor VIIa; D013917:Thrombin