title: Peroxynitrite scavenging activity of lithospermate B from Salvia miltiorrhiza.
abstract: Peroxynitrite (ONOO-) is produced by the reaction of superoxide (O2-) with nitric oxide. ONOO- damages proteins through nitration or oxidation. For protection from ONOO- induced protein modifications, ONOO- scavengers should be supplemented. Evidence was obtained that lithospermate B extracted from Salvia miltiorrhiza showed the strongest scavenging activity among its constituents. Its ONOO- scavenging activity is via an electron donation mechanism. A dihydroxyl group and a double bond seem to be essential structure requirements. The data from the experiments further confirmed a protective effect of lithospermate B on bovine serum albumin and low-density lipoprotein against ONOO-. This study demonstrated that lithospermate B with hydroxyl groups and double bonds exerts an anti-nitration effect by scavenging ONOO-.
authors: Department of Pharmacy, Pusan National University, Busan, Korea.
authors: Do You
authors: DY
authors: Soung
authors: Sook Hee
authors: SH
authors: Rhee
authors: Jung Sun
authors: JS
authors: Kim
authors: Ji Young
authors: JY
authors: Lee
authors: Hee-Sun
authors: HS
authors: Yang
authors: Jae Sue
authors: JS
authors: Choi
authors: Takako
authors: T
authors: Yokozawa
authors: Yong Nam
authors: YN
authors: Han
authors: Hae Young
authors: HY
authors: Chung
mesh_terms: D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; C061091:lithospermate B; D030421:Peroxynitrous Acid; D012710:Serum Albumin, Bovine