title: 5-HT at hypoglossal motor nucleus and respiratory control of genioglossus muscle in anesthetized rats.
abstract: Serotonin (5-HT) from medullary raphe neurons excites hypoglossal motoneurons innervating genioglossus (GG) muscle. Since some raphe neurons also show increased activity in hypercapnia, we tested the hypothesis that serotonergic mechanisms at the hypoglossal motor nucleus (HMN) modulate GG activity and responses to CO2. Seventeen urethane-anesthetized, tracheotomized and vagotomized rats were studied. Microdialysis probes were used to deliver mianserin (5-HT receptor antagonist, 0 and 0.1 mM) or 5-HT (eight doses, 0-50 mM) to the HMN during room air or CO2-stimulated breathing. Mianserin decreased respiratory-related GG activity during room air and CO2-stimulated breathing (P<0.001), and also suppressed GG responses to CO2 (P=0.05). In contrast, GG activity was increased by 5-HT at the HMN, and was further increased in hypercapnia (P<0.02). However, 5-HT increased respiratory-related GG activity at levels lower (1 mM) than those eliciting tonic GG activity (10-30 mM 5-HT). The results show that 5-HT at the HMN contributes to the respiratory control of GG muscle.
authors: Department of Medicine, University of Toronto, Room 6368, Medical Sciences Building, 1 Kings College Circle, Toronto, Ont., Canada M5S 1A8.
authors: Sandeep
authors: S
authors: Sood
authors: Xia
authors: X
authors: Liu
authors: Hattie
authors: H
authors: Liu
authors: Philip
authors: P
authors: Nolan
authors: Richard L
authors: RL
authors: Horner
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000494:pharmacology; D003964:Diaphragm / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography; D004576:Electromyography; D006935:Hypercapnia / Q000469:parasitology; D007002:Hypoglossal Nerve / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D008803:Mianserin / Q000494:pharmacology; D017551:Microdialysis / Q000379:methods; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D014139:Tracheostomy / Q000379:methods; D014628:Vagotomy / Q000379:methods
chemical_list: D012702:Serotonin Antagonists; D002245:Carbon Dioxide; D008803:Mianserin; D012701:Serotonin