Expression, isolation, purification, and biochemical properties of trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16.
Here we describe cloning, expression, and purification of the enzyme trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16. Principal biochemical properties of the enzyme at different pH and temperature values were determined. Entropy and enthalpy of activation of the enzyme for substrates trehalose-6-phosphate and p-nitrophenyl glucoside were calculated, and the dependence of the kinetic parameters from ionic strength was established.
Karelov
D V
DV
Konstantinov St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina 188350, Leningrad Region, Russia.
Krasikov
V V
VV
Surjik
M A
MA
Firsov
L M
LM
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D004186:Disaccharidases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism*; D004926:Escherichia coli; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009598:Nitrophenylgalactosides / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013403:Sugar Phosphates / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D014199:Trehalose / Q000031:analogs & derivatives* / Q000378:metabolism
D013403:Sugar Phosphates; D009598:Nitrophenylgalactosides; C059200:4-nitrophenylgalactoside; C082722:trehalose-6-phosphate; D012965:Sodium Chloride; D014199:Trehalose; D005949:Glucose Oxidase; D004186:Disaccharidases; C040631:alpha, alpha-phosphotrehalase; D005947:Glucose