Overexpression of cystathionine-gamma-synthase enhances selenium volatilization in Brassica juncea.
Selenium (Se) can be assimilated and volatilized via the sulfate assimilation pathway. Cystathionine-gamma-synthase (CGS) is thought to catalyze the synthesis of Se-cystathionine from Se-cysteine, the first step in the conversion of Se-cysteine to volatile dimethylselenide. Here the hypothesis was tested that CGS is a rate-limiting enzyme for Se volatilization. Cystathionine-gamma-synthase from Arabidopsis thaliana (L.) Heynh. was overexpressed in Indian mustard [ Brassica juncea (L.) Czern & Coss], and five transgenic CGS lines with up to 10-fold enhanced CGS levels were compared with wild-type Indian mustard with respect to Se volatilization, tolerance and accumulation. The CGS transgenics showed 2- to 3-fold higher Se volatilization rates than wild-type plants when supplied with selenate or selenite. Transgenic CGS plants contained 20-40% lower shoot Se levels and 50-70% lower root Se levels than the wild type when supplied with selenite. Furthermore, CGS seedlings were more tolerant to selenite than the wild type. There were no differences in Se accumulation or tolerance from selenate, in agreement with the earlier finding that selenate-to-selenite reduction is rate-limiting for selenate tolerance and accumulation. In conclusion, CGS appears to be a rate-limiting enzyme for Se volatilization. Overexpression of CGS offers a promising approach for the creation of plants with enhanced capacity to remove Se from contaminated sites in the form of low-toxic volatile dimethylselenide.
Van Huysen
Tiffany
T
Department of Biology, Colorado State University, Anatomy/Zoology Building, Fort Collins, CO 80523, USA.
Abdel-Ghany
Salah
S
Hale
Kerry L
KL
LeDuc
Danika
D
Terry
Norman
N
Pilon-Smits
Elizabeth A H
EA
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D019757:Carbon-Oxygen Lyases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D009149:Mustard Plant / Q000201:enzymology* / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D064586:Selenic Acid; D012643:Selenium / Q000378:metabolism*; D018036:Selenium Compounds / Q000378:metabolism; D018038:Sodium Selenite / Q000378:metabolism; D014835:Volatilization
D018036:Selenium Compounds; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases; D012643:Selenium; D018038:Sodium Selenite; D064586:Selenic Acid