title: [Contribution of Parodium gel in an experimental model of human gingival inflammation].
abstract: BACKGROUND
  Our aim was to evaluate the effect of a gingival gel containing chlorhexidine and Rheum Palmatum extract on gingival fragments stimulated by SP (substance P) and lipopolysaccharides (LPS).
  
  
  METHODS
  Gingival fragments were maintained in survival for 3 days at 37 degrees C. To induce inflammation, SP and LPS were applied to the culture medium in contact with the corium. The gingival gel was applied on epithelium. Histological analysis was then performed on hematoxylin and eosin stained slides. Edema was evaluated with semi-quantitative scores. Vasodilation was studied by counting the percent of dilated vessels according to scores and the surface of these dilated vessels by morphometrical image analysis. An inflammatory cytokine, IL8, was measured in culture supernatants. Immunohistochemical expression of metalloproteinase type 9 (MMP9) implicated in inflammatory processes, was also studied (% of positive cells).
  
  
  RESULTS
  Edema, vasodilation and IL8 were significantly increased after application of SP and LPS. Application of gingival gel showed a significant decrease of these parameters. A significant decrease of MMP9 on fibroblasts and mononuclear cells was observed after use of gingival gel.
authors: Service de Stomatologie et de Chirurgie Maxillo-Faciale, Hôpital de la Pitié-Salpêtrière, 47-83 bd de l'Hôpital, 75013 Paris.
authors: S
authors: S
authors: Boisnic
authors: L
authors: L
authors: Ben Slama
authors: M-C
authors: MC
authors: Branchet-Gumila
authors: G
authors: G
authors: d'Arros
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use*; D002196:Capillaries / Q000187:drug effects; D002478:Cells, Cultured; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D004396:Coloring Agents; D004108:Dilatation, Pathologic / Q000188:drug therapy / Q000473:pathology; D005782:Gels; D005881:Gingiva / Q000098:blood supply / Q000187:drug effects*; D005891:Gingivitis / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016209:Interleukin-8 / Q000032:analysis; D008070:Lipopolysaccharides / Q000009:adverse effects; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D012250:Rheum; D013373:Substance P / Q000009:adverse effects
chemical_list: D000891:Anti-Infective Agents, Local; D004396:Coloring Agents; D005782:Gels; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013373:Substance P; D020780:Matrix Metalloproteinase 9; D002710:Chlorhexidine