Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii.
An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.
Zhu
Jun
J
Department of Microbiology, Cornell University, Ithaca, New York 14853, USA. jun_zhu@njau.edu.cn
Chai
Yunrong
Y
Zhong
Zengtao
Z
Li
Shunpeng
S
Winans
Stephen C
SC
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism*; D020561:Alphaproteobacteria / Q000254:growth & development* / Q000378:metabolism*; D027883:Astragalus Plant / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D017123:Bacteriophage T7 / Q000235:genetics / Q000378:metabolism; D001681:Biological Assay; D015964:Gene Expression Regulation, Bacterial; D009586:Nitrogen Fixation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012680:Sensitivity and Specificity; D015398:Signal Transduction
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C080512:TraR protein, Agrobacterium tumefaciens; C088386:homoserine lactone; D015107:4-Butyrolactone