title: A new gene-finding tool: using the Caenorhabditis elegans operons for identifying functional partner proteins in human cells.
abstract: How can a large number of different phenotypes be generated by a limited number of genotypes? Promiscuity between different, structurally related and/or unrelated proteins seems to provide a plausible explanation to this pertinent question. Strategies able to predict such functional interrelations between different proteins are important to restrict the number of putative candidate proteins, which can then be subjected to time-consuming functional tests. Here we describe the use of the operon structure of the nematode genome to identify partner proteins in human cells. In this work we focus on ion channels proteins, which build an interface between the cell and the outside world and are responsible for a growing number of diseases in humans. However, the proposed strategy for the partner protein quest is not restricted to this scientific area but can be adopted in virtually every field of human biology where protein-protein interactions are assumed.
authors: Institute of Physiology, University of Innsbruck, Fritz-Pregl Strasse 3, A-6020 Innsbruck, Austria.
authors: Sonja
authors: S
authors: Eichmüller
authors: Valeria
authors: V
authors: Vezzoli
authors: Claudia
authors: C
authors: Bazzini
authors: Markus
authors: M
authors: Ritter
authors: Johannes
authors: J
authors: Fürst
authors: Martin
authors: M
authors: Jakab
authors: Andrea
authors: A
authors: Ravasio
authors: Sabine
authors: S
authors: Chwatal
authors: Silvia
authors: S
authors: Dossena
authors: Guido
authors: G
authors: Bottà
authors: Giuliano
authors: G
authors: Meyer
authors: Brigitte
authors: B
authors: Maier
authors: Giovanna
authors: G
authors: Valenti
authors: Florian
authors: F
authors: Lang
authors: Markus
authors: M
authors: Paulmichl
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D016678:Genome; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D007477:Ions / Q000737:chemistry / Q000378:metabolism; D008027:Light; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D009876:Operon; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D007473:Ion Channels; D007477:Ions; D011993:Recombinant Fusion Proteins; D000255:Adenosine Triphosphate