title: DNase I treatment of cDNA first strands prevents RT-PCR amplification of contaminating DNA sequences.
authors: Center for Human Genetics, University of Bremen, Bremen, Germany.
authors: Aljoscha M
authors: AM
authors: Flohr
authors: Thomas
authors: T
authors: Hackenbeck
authors: Claudia
authors: C
authors: Schlueter
authors: Piere
authors: P
authors: Rogalla
authors: Joern
authors: J
authors: Bullerdiek
mesh_terms: D000595:Amino Acid Sequence; D016477:Artifacts; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism*; D004340:Drug Contamination / Q000517:prevention & control*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011786:Quality Control; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D018076:DNA, Complementary; D003850:Deoxyribonuclease I