title: High prevalence of epidermodysplasia verruciformis-associated human papillomavirus DNA in actinic keratoses of the immunocompetent population.
abstract: Skin cancers in both immunosuppressed and immunocompetent populations are associated with epidermodysplasia verruciformis human papillomavirus (EV-HPV) DNA. However, little is known about the prevalence of EV-HPVs in actinic keratoses in immunocompetent individuals. Actinic keratoses from 114 patients were classified as low-grade ( n=76) or high-grade ( n=38) according to the extent of histological atypia. HPV DNA was amplified from 54 frozen and 60 paraffin-embedded biopsy specimens by nested polymerase chain reaction (PCR) with several consensus and type-specific primers. PCR products were sequenced for typing. These results were compared with HPV detection in skin cancers ( n=20) and Bowen's disease ( n=18). A broad spectrum of EV-HPV types including oncogenic HPV5 and HPV8 and partially characterized sequences were detected in actinic keratoses and cutaneous cancers. In actinic keratoses a higher prevalence of EV-HPV DNA was found in frozen tissues than in formalin-fixed tissues (85% vs 67%). There was no difference between the low- and high-grade actinic keratoses either in terms of EV-HPV DNA prevalence or the results of serological study using HPV8 virus-like particles. The detection rate of EV-HPVs was lower in skin cancers and Bowen's disease. This would suggest involvement of EV-HPVs in the early stages of cutaneous oncogenesis.
authors: Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, 50935 Cologne, Germany. herbert.pfister@medizin.uni-koeln.de
authors: Herbert
authors: H
authors: Pfister
authors: Pawel G
authors: PG
authors: Fuchs
authors: Slawomir
authors: S
authors: Majewski
authors: Stefania
authors: S
authors: Jablonska
authors: Iwona
authors: I
authors: Pniewska
authors: Magdalena
authors: M
authors: Malejczyk
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D001913:Bowen's Disease / Q000378:metabolism / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000821:virology; D004279:DNA, Viral / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004819:Epidermodysplasia Verruciformis / Q000821:virology*; D006801:Humans; D007642:Keratosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011832:Radiation Injuries / Q000150:complications; D012878:Skin Neoplasms / Q000378:metabolism / Q000821:virology; D014466:Ultraviolet Rays
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral