Alternative NMR method for quantitative determination of acyl positional distribution in triacylglycerols and related compounds.
High-resolution 13C NMR spectroscopy has been used to analyze the positional distribution of fatty acids in model triacylglycerols. A novel method for quantitative determination of the positional distribution of unsaturated chains in triacylglycerols simultaneously with the ratio of saturated/unsaturated acyl chains has been proposed, utilizing the chemical shift differences of the aliphatic atoms C4, C5, and C6. The use of HSQC-TOCSY spectra allows unequivocal proof of the position of the unsaturated chain as well as complete assignment of the 13C NMR signals in tripalmitin.
Simova
S
S
Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. sds@orgchm.bas.bg
Ivanova
G
G
Spassov
S L
SL
D000215:Acylation; D002247:Carbon Isotopes; D005227:Fatty Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013237:Stereoisomerism; D014280:Triglycerides / Q000737:chemistry*
D002247:Carbon Isotopes; D005227:Fatty Acids; D014280:Triglycerides; C005859:tripalmitin