Dependence of leukemic cell autofluorescence patterns on the degree of differentiation.
The characterisation of leukemic cell autofluorescence during differentiation, induced by 12-O-tetradecanoylphorbol 13-acetate and all-trans retinoic acid, was performed by autofluorescence microspectroscopy and multispectral imaging autofluorescence microscopy. We have found that a dependence exists between the cell autofluorescence pattern and the degree of cell differentiation. When cells differentiate, their autofluorescence emission changes, following the morphological and functional rearrangement of cell structures. A decrease in emission intensity and a different distribution of endogenous fluorophores are observed. Thus, autofluorescence monitoring on living cells is a potentially useful tool for in vitro study of the differentiation processes. Furthermore, different maturation steps can be distinguished on the basis of the cell fluorescence pattern, leading the way to future application of the technique in diagnostics.
Monici
Monica
M
Centre of Excellence in Optronics, Largo Enrico Fermi, 6, 50125 Florence, Italy. monici@ino.it
Agati
Giovanni
G
Fusi
Franco
F
Pratesi
Riccardo
R
Paglierani
Milena
M
Santini
Valeria
V
Bernabei
Pietro Antonio
PA
D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D005453:Fluorescence; D018922:HL-60 Cells; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology*; D008856:Microscopy, Fluorescence; D018462:Photobiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine