Inhibition study of angiotensin converting enzyme by capillary electrophoresis after enzymatic reaction at capillary inlet.
Capillary electrophoresis was used to study the inhibition of angiotensin-converting enzyme (ACE) by different inhibitors. Reaction occurred at the capillary inlet during a predetermined waiting period, followed by the electrophoretic separation of the reaction compounds. ACE activity was determined by the quantification of the reaction product, hippuric acid, at 230 nm. The technique was used to study the potency of five different inhibitors (captopril, lisinopril, perindoprilat, quinaprilat and benazeprilat). During a kinetic study, the Ki value of captopril was estimated to be 55.4 +/- 8.8 nM, a value consistent with previously reported values.
Van Dyck
Sigrid
S
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.
Nováková
Sona
S
Van Schepdael
Ann
A
Hoogmartens
Jos
J
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007700:Kinetics; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects* / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D007703:Peptidyl-Dipeptidase A