Evaluation of a 24-hour bioluminescent enzyme immunoassay for the rapid detection of Salmonella in chicken carcass rinses.
A bioluminescent enzyme immunoassay (BEIA), using Salmonella-specific monoclonal antibody M183 for capture and biotinylated monoclonal antibody M183 for detection, was developed with InteLite AB streptavidin-biotinylated firefly luciferase complex as a reporter. Salmonella cultures were preenriched in buffered peptone water with shaking for 6 h at 37 degrees C and then selectively enriched in Muller-Kauffmann tetrathionate (MKTT) broth and modified semisolid Rappaport-Vassiliadis (MSRV) medium for 16 h at 42 degrees C. After enrichment, the total test time for the BEIA was 1.5 h. The analytical sensitivity of the BEIA ranged from 6.0 x 10(2) CFU/ml to 1.2 x 10(5) CFU/ml in MKTT and from 1.4 x 10(5) to 2.3 x 10(6) CFU/ml in MSRV using six Salmonella serovars prevalent in Canada. With enrichment cultures, the BEIA detected 1 CFU of Salmonella Typhimurium and Salmonella Enteritidis in 25 ml of chicken rinses. Representative strains of 10 Salmonella serovars were detected, and cross-reactivity was not observed with 25 non-Salmonella foodborne bacteria. The BEIA performance was assessed by testing 420 poultry samples, which were analyzed in parallel with the standard MSRV culture method. The BEIA detected 117 (27.88%) Salmonella-positive samples, whereas the standard MSRV culture method detected 124 (29.5%). The BEIA had a sensitivity of 64.5% and a specificity of 87.5% compared to the standard MSRV culture method. However, similar specificities and sensitivities were obtained when the standard MSRV culture method was compared to the BEIA (sensitivity = 68.4% and specificity = 85.5%). Neither method detected 100% of the Salmonella found in the samples tested, and statistical analyses indicated no significant difference between the two methods. In summary, the BEIA offers another alternative for the detection of Salmonella, with the additional advantage of providing a 24-h test for detecting Salmonella in chicken carcass rinses. The results obtained in this research indicate that tests are still needed for the isolation and detection of Salmonella that will establish the true prevalence of Salmonella in chicken samples.
Valdivieso-Garcia
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Laboratory for Foodborne Zoonoses, Population and Public Health Branch, Health Canada, 110 Stone Road West, Guelph, Ontario, Canada N1G 3W4. alfonso_valdivieso@hc-sc.gc.ca
Desruisseau
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A
Riche
E
E
Fukuda
S
S
Tatsumi
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001431:Bacteriological Techniques; D019921:Biotinylation; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods; D003429:Cross Reactions; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D007124:Immunoenzyme Techniques / Q000379:methods*; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D000911:Antibodies, Monoclonal; D003470:Culture Media