Evaluation of the expression of collagen type I in porous calcium phosphate ceramics implanted in an extra-osseous site.
The aim of the present study is to demonstrate the newly formed tissue in calcium phosphate (Ca/P) ceramics after extra-osseous implantation by histological and immunohistochemistry (IHC) methods. Synthesis porous Ca/P ceramics without adding any growth factor and living cell were implanted in the dorsal muscle of dogs for 1 and 2 months. Undecalcified and decalcified sections were stained by hematoxylin and eosin (H&E), and IHC, respectively. The histological results showed the beginning of osteogenesis and angiogenesis after being implanted for 1 month and the obvious new bone formation after being implanted for 2 months. IHC were conducted via the avidin-biotin peroxidase complex (ABC) method and the primary antibody was collagen type I. IHC results indicated that collagen type I was expressed within osteoblast-like cells and newly formed bone-like tissue in Ca/P ceramics after 1 month, and in the mineralized matrix of newly formed bone and osteoblasts, some osteocytes and some lacunae after 2 months. No cartilage and chondrocytes were observed in the histological and IHC-stained sections. Evidence of intramembranous osteogenesis was confirmed.
Qu
S X
SX
School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, China. mwbqshx@sina.com
Guo
X
X
Weng
J
J
Cheng
J C Y
JC
Feng
B
B
Yeung
H Y
HY
Zhang
X D
XD
D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry; D024042:Collagen Type I / Q000378:metabolism*; D004285:Dogs; D008422:Materials Testing; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology* / Q000601:surgery; D018919:Neovascularization, Physiologic / Q000502:physiology; D016348:Osseointegration / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D019736:Prostheses and Implants
D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; C020243:calcium phosphate