The EOL-1 cell line as an in vitro model for the study of FIP1L1-PDGFRA-positive chronic eosinophilic leukemia.
We recently identified the chimeric kinase FIP1L1-platelet-derived growth factor receptor alpha (PDGFRalpha) as a cause of the hypereosinophilic syndrome and of chronic eosinophilic leukemia. To investigate the role of FIP1L1-PDGFRA in the pathogenesis of acute leukemia, we screened 87 leukemia cell lines for the presence of FIP1L1-PDGFRA. One cell line, EOL-1, expressed the FIP1L1-PDGFRA fusion. Three structurally divergent kinase inhibitors--imatinib (STI-571), PKC412, and SU5614--inhibited the growth of EOL-1 cells. These results indicate that the fusion of FIP1L1 to PDGFRA occurs rarely in leukemia cell lines, but they identify EOL-1 as an in vitro model for the study of FIP1L1-PDGFRA-positive chronic eosinophilic leukemia and for the analysis of small molecule inhibitors of FIP1L1-PDGFRalpha.
Cools
Jan
J
Division of Hematology and the Howard Hughes Medical Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. jan.cools@med.kuleuven.ac.be
Quentmeier
Hilmar
H
Huntly
Brian J P
BJ
Marynen
Peter
P
Griffin
James D
JD
Drexler
Hans G
HG
Gilliland
D Gary
DG
D017209:Apoptosis; D001483:Base Sequence; D001549:Benzamides; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000235:genetics*; D000068877:Imatinib Mesylate; D008954:Models, Biological; D010879:Piperazines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000032:analysis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D039221:mRNA Cleavage and Polyadenylation Factors / Q000032:analysis* / Q000235:genetics
D001549:Benzamides; D015415:Biomarkers; D017931:DNA Primers; D004791:Enzyme Inhibitors; C479818:FIP1L1 protein, human; D010879:Piperazines; D011743:Pyrimidines; D011993:Recombinant Fusion Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D000068877:Imatinib Mesylate; D020796:Receptor, Platelet-Derived Growth Factor alpha