The production of 6-deoxy-L-talan from Actinobacillus actinomycetemcomitans via bacterial coupling in vitro.
A method of producing 6-deoxy-L-talan, the serotype c-specific polysaccharide antigen (SPA) from Actinobacillus actinomycetemcomitans,was established using a whole-cell reaction with two recombinant Escherichia coli strains. The production of serotype c-SPA was investigated using the dot blot assay with anti-A. actinomycetemcomitans NCTC 9710 serum after an 18-h reaction, starting with a solution containing the recombinant E. coli cells, alpha-d-glucose-1-phosphate, and dTTP. Moreover, examination of the time course for 6-deoxy-L-talan production proved that this system ran satisfactorily. This paper is the first report of a convenient method to readily produce the exopolysaccharide from A. actinomycetemcomitans in vitro.
Suzuki
N
N
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka, Japan.
Nakano
Y
Y
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011134:Polysaccharides / Q000096:biosynthesis / Q000235:genetics*; D013942:Thymine Nucleotides / Q000378:metabolism; D014169:Transformation, Bacterial / Q000235:genetics*
C070694:6-deoxytalan; D000942:Antigens, Bacterial; D005958:Glucosephosphates; D011134:Polysaccharides; D013942:Thymine Nucleotides; C031590:glucose-1-phosphate; C024157:thymidine 5'-triphosphate