Dynamic properties of soy globulin adsorbed films at the air-water interface.
In this paper we present surface dilatational properties of soy globulins (beta-conglycinin, glycinin, and reduced glycinin with 10 mM of dithiothreitol (DTT)) adsorbed onto the air-water interface, as a function of adsorption time. The experiments were performed at constant temperature (20 degrees C), pH (8.0), and ionic strength (0.05 M). The surface rheological parameters were measured as a function of protein concentration (ranging from 1 to 1x10(-3)% wt/wt). We found that the surface dilatational modulus, E, increases, and the phase angle, phi, decreases with time, theta, which may be associated with protein adsorption. These phenomena have been related to protein adsorption, unfolding, and/or protein-protein interactions (at long-term adsorption) as a function of protein concentration in solution. From a rheological point of view, the surface viscoelastic characteristics of soy globulin films adsorbed at the air-water interface are practically elastic. The main conclusion is that the dilatational properties of the adsorbed films depend on the molecular structure of the protein.
Rodríguez Patino
Juan M
JM
Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, c. Prof. García González, s/núm. 41012, Sevilla, Spain. jmrodri@us.es
Molina Ortiz
Sara E
SE
Carrera Sánchez
Cecilio
C
Rodríguez Niño
Maria Rosario
MR
Añón
Maria Cristina
MC
D000327:Adsorption; D000388:Air; D052179:Antigens, Plant; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004229:Dithiothreitol / Q000737:chemistry* / Q000378:metabolism; D005916:Globulins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D009040:Motion Pictures; D012212:Rheology; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D014867:Water
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D014867:Water; C011474:glycinin; D004229:Dithiothreitol