PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle.
The use of a single restriction fragment length polymorphism (RFLP)-PCR assay which is able to characterise all important bovine trypanosome species was evaluated for the detection of mixed infections with Trypanosoma brucei brucei, Trypanosoma theileri, Trypanosoma congolense and Trypanosoma vivax. Results showed that mixed infections are detectable at a minimum ratio of 2%/98% of standardised DNA solutions with a concentration of 10 ng ml(-1). All mixed infections gave clear profiles that could be easily differentiated except with T. theileri and T. congolense where the T. theileri band was concealed by the T. congolense profile.
Delespaux
V
V
Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. vdelespaux@itg.be
Ayral
F
F
Geysen
D
D
Geerts
S
S
D000818:Animals; D002417:Cattle; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014345:Trypanosoma / Q000145:classification* / Q000302:isolation & purification*; D014346:Trypanosoma brucei brucei / Q000145:classification / Q000302:isolation & purification; D014348:Trypanosoma congolense / Q000145:classification / Q000302:isolation & purification; D016689:Trypanosoma vivax / Q000145:classification / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis* / Q000469:parasitology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal