Quantitative analysis of vitamin A degradation by Raman spectroscopy.
Vitamin A is known to support cell growth promotion, maintenance, and differentiation of epithelial tissues. Retinol is currently used in cosmetic formulations and products to deliver these and other benefits to the skin. However, retinol is known to be unstable and, therefore, remains of great concern to the cosmetic industry. The decomposition pathways of the retinoids in general have been previously postulated and investigated mostly by high-performance liquid chromatography (HPLC) with UV/Vis spectroscopy. In our studies, we examined specific conditions at which retinol degrades and subsequently identified and quantified the products of retinol decomposition by Raman spectroscopy. We reveal which experimental settings and computational tools allow monitoring of in situ evolution of an all-trans retinol solution when submitted to UV light in the presence of oxygen.
Failloux
Nelly
N
Equipe des systèmes d'intérêts biologiques, Laboratoire de Dynamique Interactions et Réactivité, CNRS-UMR 7075, 2 rue Henri Dunant, 94 320 Thiais, France.
Bonnet
Isabelle
I
Baron
Marie-Hélène
MH
Perrier
Eric
E
D019840:2-Propanol / Q000737:chemistry; D003358:Cosmetics / Q000032:analysis / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D004355:Drug Stability; D004356:Drug Storage; D009824:Ointments / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects*
D003358:Cosmetics; D009824:Ointments; D014801:Vitamin A; D019840:2-Propanol; D010100:Oxygen