Comparison between indirect immunofluorescence assay and shell vial culture for detection of mumps virus from clinical samples.
We report a prospective comparison of the efficacies of an indirect immunofluorescence assay (IFA) and shell vial culture (SVC) of throat swab and urine samples from patients with mumps. Throat swab samples were used for the IFA; the urine samples and throat swabs were inoculated into vials of Vero cells. We studied 62 patients by using 62 throat swabs and 50 urine samples (50 patients with both samples). Sixty (96.7%) throat samples were positive in the SVC, and 61 (98.3%) were positive in the IFA. For the 50 patients from whom both samples were available, the IFA was positive in 50 (100%) cases, the urine sample was positive in 49 (98%) cases, and the throat swab was positive in 48 (96%) cases (P > 0.05). This comparison of throat swabs and urine samples has shown that the two clinical samples are similar in efficacy.
Reina
Jordi
J
Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta (Universitat Illes Balears), 07014 Palma de Mallorca, Spain. jreina@hsd.es
Ballesteros
Francisca
F
Ruiz de Gopegui
Enrique
E
Munar
Maria
M
Mari
Margarita
M
D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D009107:Mumps / Q000652:urine / Q000821:virology*; D009109:Mumps virus / Q000302:isolation & purification*; D010614:Pharynx / Q000821:virology; D015203:Reproducibility of Results; D014773:Virology / Q000379:methods