title: [Effect of PSK on Th1/Th2 balance in tumor-bearing mice].
abstract: We investigated the effect of PSK on Th1/Th2 balance in tumor-bearing mice. PSK was intraperitoneally administered to Meth A-bearing BALB/c mice, and PSK caused regression of the Meth A tumor. The results of Winn assay suggested that the effect of PSK was dependent on CD4+T cells. Furthermore, spleen cells were cultured with mitomycin C-treated Meth A, after which the cytokine concentration was measured by ELISA. IFN-gamma production was increased and IL-4 showed almost no change in PSK-administered mice. In another experiment, PSK was orally administered to colon 26-bearing mice in which tumors were inoculated into the subserosal space of the cecum. Mesenteric lymph nodes cells were cultured with mitomycin C-treated colon 26 cells. IFN-gamma production was increased, but not so much as to be statistically significant, and IL-4 was significantly decreased in PSK-administered mice. PSK increased IFN-gamma and IL-12 p70 production and decreased IL-4 production when spleen cells were stimulated with Con A together with PSK in vitro. As suggested from these results, PSK might induce cytokine production that works for Th1 differentiation, and suppress cytokine production that works for Th2 differentiation, and shift the Th1/Th2 balance toward Th1 dominance in tumor-bearing mice.
authors: Biomedical Research Laboratory, Kureha Chemical Industry Co., Ltd.
authors: Tsutomu
authors: T
authors: Wada
authors: Hiroko
authors: H
authors: Iijima
authors: Fujio
authors: F
authors: Sekine
authors: Mariko
authors: M
authors: Kato
authors: Momomi
authors: M
authors: Tsugane
authors: Yoshiharu
authors: Y
authors: Oguchi
authors: Kenichi
authors: K
authors: Matsunaga
authors: Takao
authors: T
authors: Ando
authors: Kikuo
authors: K
authors: Nomoto
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D005354:Fibrosarcoma / Q000276:immunology / Q000473:pathology*; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011509:Proteoglycans / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000903:Antibiotics, Antineoplastic; D011509:Proteoglycans; D018664:Interleukin-12; D015847:Interleukin-4; C010770:polysaccharide-K; D007371:Interferon-gamma