[Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].
OBJECTIVE
To improve SEA-ELISA, an immunodiagnostic assay for schistosomiasis.


METHODS
Soluble egg antigen (SEA) of Schistosoma japonicum was treated with sodium periodate (SP) in order to oxidate its glycosylated epitopes. ELISA using the treated SEA was then performed to detect specific antibodies to SEA in the sera of schistosomiasis patients.


RESULTS
Serum samples were tested by ELISA using SEA treated with sodium periodate (SP-SEA-ELISA), including 64 sera from cases with chronic schistosomiasis japonica, 119 sera from normal individuals in non-endemic area, 34 sera from patients with clonorchiasis, 33 sera of paragonimiasis cases and 36 sera from patients with cysticercosis. The results showed that its sensitivity (98.4%) was similar to that of the routine SEA-ELISA (100.0%) (P > 0.05) and the specificity is higher than that of the SEA-ELISA (P < 0.05). SP-SEA-ELISA showed a higher negative rate (89.0%) for sera of schistosomiasis patients 12 months post-treatment than that of the SEA-ELISA (42.1%).


CONCLUSIONS
Use of SP-SEA can increase the specificity of ELISA, reduce cross-reactivity with serum samples from cases infected with other parasites and improve its value in evaluating therapeutic efficacy.
Huang
Yue-long
YL
Department of Parasitology, Xiangya Medical College Central South University, Changsha 410078.
Yi
Xin-yuan
XY
Zeng
Xian-fang
XF
Zhang
Ran
R
Yuan
Shi-shan
SS
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000918:Antibody Specificity; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010504:Periodic Acid; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010504:Periodic Acid; C009288:metaperiodate