Microfluidic systems in proteomics.
We present the state-of-the-art in miniaturized sample preparation, immunoassays, one-dimensional and multidimensional analyte separations, and coupling of microdevices with electrospray ionization-mass spectrometry. Hyphenation of these different techniques and their relevance to proteomics will be discussed. In particular, we will show that analytical performances of microfluidic analytical systems are already close to fulfill the requirements for proteomics, and that miniaturization results at the same time in a dramatic increase in analysis throughput. Throughout this review, some examples of analytical operations that cannot be achieved without microfluidics will be emphasized. Finally, conditions for the spreading of microanalytical systems in routine proteomic labs will be discussed.
Lion
Niels
N
Laboratoire d'Electrochimie Physique et Analytique (LEPA), Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Rohner
Tatiana C
TC
Dayon
Loïc
L
Arnaud
Isabelle L
IL
Damoc
Eugen
E
Youhnovski
Nikolay
N
Wu
Zhi-Yong
ZY
Roussel
Christophe
C
Josserand
Jacques
J
Jensen
Henrik
H
Rossier
Joël S
JS
Przybylski
Michael
M
Girault
Hubert H
HH
D004586:Electrophoresis / Q000379:methods; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D044085:Microfluidics / Q000379:methods*; D008904:Miniaturization / Q000379:methods; D020543:Proteome
D004798:Enzymes; D020543:Proteome