title: Less impaired cell-mediated immune response in the murine peritoneal cavity after CO(2) pneumoperitoneum.
abstract: OBJECTIVE
  We compared changes in the populations of peritoneal T lymphocytes and natural killer (NK) cells after CO(2) pneumoperitoneum and laparotomy to clarify whether pneumoperitoneum affects cell-mediated immune responses in the peritoneal cavity.
  
  
  METHODS
  We analyzed and compared populations of T lymphocytes and NK cells among peritoneal exudative cells (PECs) collected from 185 female mice subjected to pneumoperitoneum, laparotomy, or anesthesia only. PECs were collected postoperatively, and the populations of T lymphocytes and NK cell subsets were analyzed by flow cytometry. The NK cell cytotoxicity (NKCC) of PECs and splenocytes was measured.
  
  
  RESULTS
  The populations of CD3(+), CD4(+), and CD8(+) lymphocytes in the PECs continued to increase up until postoperative day (POD) 7 after laparotomy. The CD4/8 ratio on POD 3 was significantly lower after laparotomy than after pneumoperitoneum. The percentages of NK cells in the pneumoperitoneum group were significantly lower than those in the laparotomy group. On POD 1, the NKCC of splenocytes was less impaired in the pneumoperitoneum group than in the laparotomy group (10.3% vs 5.0%, P << 0.05).
  
  
  CONCLUSIONS
  Laparoscopic surgery is preferable to open surgery because it results in less impairment of systemic and intraperitoneal cell-mediated immune responses.
authors: First Department of Surgery, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, 879-5593 Oita, Japan.
authors: Kyuzo
authors: K
authors: Fujii
authors: Koichi
authors: K
authors: Izumi
authors: Kazuya
authors: K
authors: Sonoda
authors: Norio
authors: N
authors: Shiraishi
authors: Yosuke
authors: Y
authors: Adachi
authors: Seigo
authors: S
authors: Kitano
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology; D010535:Laparoscopy / Q000009:adverse effects / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000601:surgery; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects / Q000379:methods*; D011336:Probability; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
chemical_list: D002245:Carbon Dioxide