Antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses.
We demonstrated that the long tandemly repeated region (TR-2) is dispensable for in vitro replication of fowl adenovirus 9 (FAdV-9). The TR-2-deleted recombinant FAdV-9 expressing the enhanced green fluorescence protein was further characterized for in vivo effects. Groups of chickens were exposed to recombinant or wild-type FAdV-9 by intramuscular injection, through the feed or drinking water and one group served as a negative control. The antibody (Ab) response, evaluated by ELISA and a plaque reduction test depended on the virus, dosage and the route of inoculation. Although the highest levels of anti-viral Ab were detected in chickens inoculated intramuscularly (i.m.) with wild-type FAdV-9, the deletion of TR-2 did not have a significant effect on the immune response. The tissue distribution of the virus was examined by the polymerase chain reaction (PCR) and was similar for both wild-type and recombinant viruses. Based on these results the TR-2 was dispensable for viral replication in vivo and did not influence virus distribution, and the recombinant FAdV-9 induced the same immune response as the wild-type virus.
Ojkic
Davor
D
Animal Health Laboratory, Laboratory Services Division, University of Guelph, Box 3612, Ontario N1H 6R8, Guelph, Canada.
Nagy
Eva
E
D000257:Adenoviridae Infections / Q000276:immunology* / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation / Q000276:immunology; D002645:Chickens; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D025681:Fowl adenovirus A / Q000276:immunology*; D049452:Green Fluorescent Proteins; D007273:Injections, Intramuscular; D008164:Luminescent Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020080:Tandem Repeat Sequences; D014614:Vaccines, Synthetic / Q000276:immunology; D019562:Viral Load; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D017931:DNA Primers; D008164:Luminescent Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D049452:Green Fluorescent Proteins