Inhibiting effects of Achyranthes bidentata polysaccharide and Lycium barbarum polysaccharide on nonenzyme glycation in D-galactose induced mouse aging model.
OBJECTIVE
To investigate the inhibiting effects and mechanism of achyranthes bidentata polysaccharide (ABP) and lycium barbarum polysaccharide (LBP) on nonenzyme glycation in D-galactose induced mouse aging model.


METHODS
Serum AGE levels were determined by AGE-ELISA, MTT method was used to determine lymphocyte proliferation, IL-2 activity was determined by a bioassay method. Spontaneous motor activity was used to detect mouse's neuromuscular movement, latency of step-through method was used to examine learning and memory abilities of mouse, colormetric assay was used to determine hydroxyproline concentration in mouse skin, pyrogallol autoxidation method was used to determine superoxide dismutase (SOD) activity of erythrocytes.


RESULTS
Decreased levels of serum AGE, hydroxyproline concentration in mouse skin and spontaneous motor activity in D-galactose mouse aging model were detected after treated with ABP or LBP, while lymphocyte proliferation and IL-2 activity, learning and memory abilities, SOD activity of erythrocytes, were enhanced.


CONCLUSIONS
ABP and LBP could inhibit nonenzyme glycation in D-galactose induced mouse aging model in vivo and ABP has a better inhibiting effect than LBP.
Deng
Hong-Bin
HB
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Cui
Da-Peng
DP
Jiang
Jian-Ming
JM
Feng
Yan-Chun
YC
Cai
Nian-Sheng
NS
Li
Dian-Dong
DD
D029044:Achyranthes / Q000737:chemistry*; D000375:Aging / Q000502:physiology*; D000818:Animals; D004195:Disease Models, Animal; D004912:Erythrocytes; D005260:Female; D005690:Galactose / Q000737:chemistry*; D007858:Learning; D032305:Lycium / Q000737:chemistry*; D008568:Memory; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D011134:Polysaccharides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D011134:Polysaccharides; D013482:Superoxide Dismutase; D005690:Galactose