Protein A-tagging for purification of native macromolecular complexes from Candida albicans.
Protein A-tagging has become an important tool in characterization of protein-protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A-tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox.
Blackwell
Chris
C
School of Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle upon Tyne, UK.
Russell
Claire L
CL
Argimon
Silvia
S
Brown
Alistair J P
AJ
Brown
Jeremy D
JD
D001483:Base Sequence; D015153:Blotting, Western; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D018271:Signal Recognition Particle / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics
D005656:Fungal Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D018271:Signal Recognition Particle; D013205:Staphylococcal Protein A