title: Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans.
abstract: During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of their ends, but we know little about the structure of MT ends in spindles. Here, we use high-voltage electron tomography to study the centrosome- and kinetochore-associated ends of spindle MTs in embryonic cells of the nematode, Caenorhabditis elegans. Centrosome-associated MT ends are either closed or open. Closed MT ends are more numerous and are uniformly distributed around the centrosome, but open ends are found preferentially on kinetochore-attached MTs. These results have structural implications for models of MT interactions with centrosomes.
authors: Boulder Laboratory for 3-D Electron Microscopy of Cells, University of Colorado, 80309, USA.
authors: Eileen T
authors: ET
authors: O'Toole
authors: Kent L
authors: KL
authors: McDonald
authors: Jana
authors: J
authors: Mäntler
authors: J Richard
authors: JR
authors: McIntosh
authors: Anthony A
authors: AA
authors: Hyman
authors: Thomas
authors: T
authors: Müller-Reichert
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism / Q000648:ultrastructure*; D002502:Centrioles / Q000502:physiology / Q000648:ultrastructure; D018385:Centrosome / Q000502:physiology / Q000648:ultrastructure*; D002843:Chromatin / Q000502:physiology / Q000648:ultrastructure; D018386:Kinetochores / Q000502:physiology / Q000648:ultrastructure; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D008938:Mitosis / Q000502:physiology*; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure; D014057:Tomography, X-Ray Computed
chemical_list: D002843:Chromatin