Replication and expression of a swinepox virus vector delivering feline leukemia virus Gag and Env to cell lines of swine and feline origin.
The host range of swinepox virus (SPV) is restricted to swine, although SPV has been shown to infect mammalian, non-swine cells, without recovery of infectious virus. SPV is a reasonable candidate for development as a non-productively replicating viral vector for use in non-swine, mammalian species, such as the cat. A novel SPV gene deletion (SPV 043) was created and found to be non-attenuating. This deletion was utilized to generate a stable recombinant virus expressing the Gag-Pro and Env proteins of feline leukemia virus (FeLV). Expression and replication of this vector was studied in embryonic swine kidney cells (ESK-4), and two feline cell lines, Crandell feline kidney cells (CRFK) and feline skin fibroblasts (FSF). Our results showed that feline cells were susceptible to infection by SPV and supported expression of foreign genes driven by synthetic poxvirus promoters, however, SPV viral DNA was not replicated in feline cells and infectious virus was not recovered. In addition, FeLV Gag virus-like particles were produced from both ESK-4 and CRFK cells and foreign antigens were incorporated into infectious SPV intracellular mature virions (IMV). These results suggest that SPV may have potential as a safe vaccine delivery vector for cats.
Winslow
Barbara J
BJ
Schering-Plough Animal Health Corporation, San Diego, CA 92121, USA. barbara.winslow@spcorp.com
Cochran
Mark D
MD
Holzenburg
Andreas
A
Sun
Jingchuan
J
Junker
David E
DE
Collisson
Ellen W
EW
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D015686:Gene Products, env / Q000235:genetics / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D005256:Leukemia Virus, Feline / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018148:Suipoxvirus / Q000235:genetics / Q000502:physiology; D013552:Swine; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
D015686:Gene Products, env; D015683:Gene Products, gag