Identification of Aerococcus urinae in urine samples.
To evaluate procedures for the identification of Aerococcus urinae, we examined 24 alpha-hemolytic non-enterococcal bacterial isolates from 4373 urine samples. Published procedures were compared with 16s rRNA sequencing and biochemical profiling (BBL-Crystal-GP). 16s rRNA sequencing and BBL-Crystal-GP identified the same 13 isolates as A. urinae. Published tests failed to distinguish the 13 A. urinae isolates from eight non-A. urinae isolates; several tests exhibited no discrimination. Ciprofloxacin and trimethoprim susceptibility and growth at 45 degrees C improved discrimination. For urinary isolates, standard procedures for identification of A. urinae are redundant and insufficiently discriminatory, and may need revision. BBL-Crystal-GP is an accurate alternative.
Grude
N
N
A/S Telelab, Pb 1868 Gulset, 3703 Skien, University of Tromsø, Tromsø, Norway.
Jenkins
A
A
Tveten
Y
Y
Kristiansen
B-E
BE
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009664:Norway; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D013289:Streptococcaceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S