[The modified isolation and culture method of rat Sertoli cells].
OBJECTIVE
To simplify the procedures of isolation of rat Sertoli cells and increase the yield of Sertoli cells cultured.


METHODS
Decapsulated rat testis from 16-22-day-old SD rats were digested by sequential two-step digestion using 0.25% pancreatin, 0.05% collagenase in order, and then the cell suspension was cultured in incubator under the condition of 37 degrees C and 5% CO2 for 48 h. The yields were observed and Fas Ligand (Fas-L) was tested by immunohistochemical method.


RESULTS
By the use of this modified method, the yielded sertoli cells accounted for 90% of all the cells harvested. High level expression of Fas-L was detected.


CONCLUSIONS
This modified method increases the yield of Sertoli cells and the procedures of isolation and culture are greatly simplified.
Wang
Wei
W
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Xu
Mingqing
M
Zhang
Shu
S
Yan
Lünan
L
D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology*; D013737:Testis / Q000166:cytology*