Determination of deoxyribonucleic acids by a resonance light scattering technique and its application.
For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 microg ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA.
Jie
Nianqin
N
College of Applied Chemistry, China Agricultural University, Beijing 100094, China. nqjie826@cau.edu.cn
Jia
Guifang
G
Hou
Shicong
S
Xiong
Yanmei
Y
Dong
Yanhong
Y
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes; D000073943:Neonicotinoids; D012275:Oryza / Q000737:chemistry; D009994:Osmolar Concentration; D011725:Pyridines / Q000737:chemistry; D036226:Seedlings / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D015335:Molecular Probes; D000073943:Neonicotinoids; D011725:Pyridines; C464485:acetamiprid; D004247:DNA