Uptake of Apoptotic K562 Leukaemia Cells by Immature Dendritic Cells is Greatly Facilitated by Serum.
Dendritic cells (DCs) are potent antigen-presenting cells and play an important role in T-cell-mediated immunity. DCs have been shown to induce strong antitumour immune responses both in vitro and in vivo. One way of providing the DCs with all relevant tumour antigens would be to incubate the DCs with material from dead tumour cells. We have examined the uptake of apoptotic and necrotic K562 leukaemia cells by DCs under different culture conditions. Results from coincubation experiments strongly suggested that uptake of apoptotic K562 cells was dependent upon the addition of autologous serum (AS). Under these conditions, 47-79% of all DCs were shown to ingest apoptotic material. AS also seemed to be important for the expression of functionally important markers, most notably HLA class I, CD86, CCR7 and CD83. The vast majority of DCs were shown to ingest necrotic material from K562 cells, with no additional effect of AS. The results suggest that incubation of DCs with apoptotic material for cell therapeutic purposes may best be performed in the presence of AS.
Dalgaard
J
J
Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway. jakob.dalgaard@labmed.uio.no
Beckstrøm
K J
KJ
Brinchmann
J E
JE
D017209:Apoptosis; D003713:Dendritic Cells / Q000276:immunology*; D004705:Endocytosis; D006801:Humans; D016130:Immunophenotyping; D020014:K562 Cells / Q000473:pathology*; D009336:Necrosis; D044967:Serum / Q000502:physiology*