Baicalein induced in vitro apoptosis undergo caspases activity in human promyelocytic leukemia HL-60 cells.
In this study, we evaluated the potential apoptosis effects of baicalein on human promyelocytic leukemia HL-60 cells in vitro. Apoptosis induction, cell viability, morphology and caspase-3 activity were then performed to determine by flow cytometric assay, DNA gel electrophoresis, anti-ADP-ribose stain and determination of caspase-3 activity. There is a significant difference in cell death of HL-60 cells that was detected between baicalein-treated and untreated groups. Furthermore, there was a further significant increase in apoptosis induction when cells were treated with baicalein compared to without baicalein treated groups. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed baicalein induced apoptosis in HL-60 cells. Baicalein also promoted caspase-3 activity then leading to cleavage of poly-ADP-ribose polymerase finally leading to DNA fragmentation occurrence. Furthermore, the baicalein-induced apoptosis was markedly blocked by the broad-spectrum caspase inhibitor, z-VAD-fmk. Taken together, these results suggest that treatment of human leukemia HL-60 cells with baicalein induced apoptosis through activation of caspase-3 activity.
Li
Y C
YC
Department of Medical Technology, Chungtai Institute of Health Sciences and Technology, Taichung 400, Taiwan, ROC.
Tyan
Y S
YS
Kuo
H M
HM
Chang
W C
WC
Hsia
T C
TC
Chung
J G
JG
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D020169:Caspases / Q000096:biosynthesis*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D007202:Indicators and Reagents; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014343:Trypan Blue
D000590:Amino Acid Chloromethyl Ketones; D000911:Antibodies, Monoclonal; D000975:Antioxidants; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; C105034:acetyl-aspartyl-valyl-alanyl-aspartyl fluoromethyl ketone; C006680:baicalein; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases; D014343:Trypan Blue