The effect of silica-containing calcium-phosphate particles on human osteoblasts in vitro.
There is an ongoing need for more effective and less costly bone substitutes. It has previously been proposed that silica-containing bioactive glass would be more effective as a bone repair material because of its physiochemical properties. Three newly synthesized silica-containing bioactive glass formulations, HA-31 (25%), HA-11 (50%), and HA-13 (75%), were tested as biocompatible substrates for the continued proliferation and phenotype expression of human bone cells in vitro. Two currently available bioactive glasses (BioGlass(R), Hydroxyapatite) served as comparisons. The biocompatibility of these bioglasses, as well as their osteoconductive properties, was assessed by employing primary cultures of human osteoblasts and human synoviocytes for 4 days. The results obtained demonstrated that the three new bioglasses enhanced the proliferative response of osteoblasts compared with osteoblasts cultured alone. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis indicated that osteoblasts retained their phenotypic expression by continued expression of collagen type I and alkaline phosphatase. The newly synthesized preparations of silica-containing bioactive glass did not induce stimulation of proinflammatory markers iNOS and IL-1beta in synoviocytes. In conclusion, the newly synthesized silica-containing bioactive glasses are biocompatible substrate for bone-forming osteoblasts. However, the formulations tested did not show significant advantage over the currently available bioactive glasses in vitro.
Phan
Phong V
PV
Johns Hopkins University, Department of Orthopaedic Surgery, Division of Arthritis Surgery, The Good Samaritan Hospital, 5601 Loch Raven Blvd., Baltimore, Maryland 21239, USA.
Grzanna
Mark
M
Chu
James
J
Polotsky
Anna
A
el-Ghannam
Ahmed
A
Van Heerden
David
D
Hungerford
David S
DS
Frondoza
Carmelita G
CG
D000469:Alkaline Phosphatase / Q000235:genetics; D018786:Bone Substitutes / Q000737:chemistry* / Q000592:standards; D002130:Calcium Phosphates; D002455:Cell Division; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D052247:Nitric Oxide Synthase Type II; D010006:Osteoblasts / Q000166:cytology*; D012822:Silicon Dioxide; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; D018836:Inflammation Mediators; D007375:Interleukin-1; D012822:Silicon Dioxide; C020243:calcium phosphate; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D000469:Alkaline Phosphatase