Expression of nitric oxide synthases in keratinocytes after UVB irradiation.
The importance of nitric oxide (NO) in mediating vasodilation, neurotransmission, and immune and inflammatory responses has been demonstrated. Human keratinocyte express inducible nitric oxide synthase (iNOS) and the neuronal constitutive isoform of NOS (ncNOS). We established an in vitro model in keratinocytes to investigate changes in NO, iNOS and ncNOS expression after UVB exposure. We demonstrated a large induction of NO after UVB exposure and that the source of NO produced in UVB-exposed keratinocytes was increased expression of iNOS and ncNOS. The increased NO production with increased expression of iNOS and ncNOS may contribute to the pathological and physiological features of UVB-induced erythema and skin inflammation.
Chang
Huoy-Rou
HR
Department of Biomedical Engineering, I-Shou University, Section 1, 1 Shiuecheng Road, Dashu Shiang, Kaohsiung County, Taiwan. huoy@isu.edu.tw
Tsao
Der-An
DA
Wang
Shiow-Ru
SR
Yu
Hsin-Su
HS
D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D014466:Ultraviolet Rays
D009569:Nitric Oxide; C496320:NOS1 protein, human; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II