[UV spectrophotometric research of drug mixture in sudden poisoning cases].
The objective of this research--to develop the methodics for analysis of amitriptyline, fluoxetine and codeine in the mixture.


RESULTS
The analytical method of amitriptyline, codeine and fluoxetine in the mixture identification and quantitative determination using ultraviolet spectrophotometry was established. Preparations in the mixture can't be separated, because material ultraviolet light peaks are in insufficient distance and therefore cover one another. The maximum of ultraviolet light absorption for amitriptyline is at 217-220 and 238-240 nm; fluoxetine--at 226-228 nm; codeine--at 224-248 and 284-286 nm. Using ultraviolet spectroscopy it's possible to identify amitriptyline, fluoxetine and codeine only after separating mixture components by thin-layer chromatography, the same time executing cleaning of extracts from blood and urine. Using ultraviolet spectroscopy can be identificated at least 0.5 micro g/ml amitriptyline, 1.5 micro g/ml fluoxetine and 1.0 microg/ml codeine. The intervals of the quantitative determination: 5-25 microg/ml amitriptyline; 5-30 microg/ml fluoxetine; 100-300 microg/ml codeine; relative error of the measurement, when confidence level is 95%, is from 0.66 to 1.2% for amitriptyline; from 0.66 to 1.45% for fluoxetine; from 0.33 to 0.88% for codeine. Standard deviation is from 1.15 to 2.08% for amitriptyline; from 1.15 to 2.52% for fluoxetine; from 0.57 to 1.53% for codeine. Molar absorption coefficients for all three preparations in distillated water were determinated.


CONCLUSIONS
recommended methodology suits for mixture, extracted from biological liquids, components separation, identification and quantitative determination.
Kazlauskiene
Daiva
D
Department of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Kaunas University of Medicine, Kaunas Lithuania. daivakazlauskiene@centras.lt
Vainauskas
Paulius
P
Marksiene
Rūta
R
Kiliuviene
Guoda
G
D000208:Acute Disease; D000639:Amitriptyline / Q000032:analysis* / Q000506:poisoning; D003061:Codeine / Q000032:analysis* / Q000506:poisoning; D003627:Data Interpretation, Statistical; D005473:Fluoxetine / Q000032:analysis* / Q000506:poisoning; D006801:Humans; D011041:Poisoning / Q000175:diagnosis*; D011619:Psychotropic Drugs / Q000032:analysis* / Q000506:poisoning*; D012106:Research; D013056:Spectrophotometry, Ultraviolet
D011619:Psychotropic Drugs; D005473:Fluoxetine; D000639:Amitriptyline; D003061:Codeine