title: Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.
abstract: A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
authors: Rega Institute for Medical Research, Laboratory for Medicinal Chemistry, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors: Koen
authors: K
authors: Nauwelaerts
authors: Karen
authors: K
authors: Vastmans
authors: Matheus
authors: M
authors: Froeyen
authors: Veerle
authors: V
authors: Kempeneers
authors: Jef
authors: J
authors: Rozenski
authors: Helmut
authors: H
authors: Rosemeyer
authors: Arthur
authors: A
authors: Van Aerschot
authors: Roger
authors: R
authors: Busson
authors: Jeffrey C
authors: JC
authors: Lacey
authors: Ekaterina
authors: E
authors: Efimtseva
authors: Sergey
authors: S
authors: Mikhailov
authors: Eveline
authors: E
authors: Lescrinier
authors: Piet
authors: P
authors: Herdewijn
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004256:DNA Polymerase I / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D004187:Disaccharides; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D010504:Periodic Acid; D012972:Sodium Hydroxide; D000255:Adenosine Triphosphate; D004247:DNA; C009288:metaperiodate; D004256:DNA Polymerase I