Hepatoprotective action of PGE1 analogue estimated by measuring the concentrations of acetoacetate and beta-hydroxybutyrate.
The research was carried out on 56 Chinchilla rabbits. The rabbits were divided into 8 groups with one control group. By means of stomach tube the rabbits were administered respectively: carbon tetrachloride (CCl4), synthetic analogue of prostaglandin misoprostol, CCl4 with misoprostol, indomethacin, misoprostol with indomethacin and CCl4 with misoprostol and indomethacin simultaneously. In order to confirm cytoprotective activity of prostaglandin E1 synthetic analogue in experimental acute hepatocellular damage, we measured in arterial blood the concentration of acetoacetate and beta-hydroxybutyrate and calculated molar ratio of their concentrations. The molar ratio of acetoacetate to beta-hydroxybutyrate which had been calculated for individual groups, regardless of the observed changes in ketone bodies' concentrations, in the case of rabbits which were administered CCl4 with misoprostol, had values similar to the ones obtained for the control group. The smallest value of the ketone body concentration ratio was stated at rabbits which were given only CCl4. The results of the research carried out unambiguously indicate hepatoprotective action of the synthetic analogue PGE1 which was administered to the rabbits.
Skiba
Mariusz
M
2nd Department of Internal Medicine Wojewodzki Hospital No. 4, Bytom, Poland.
Maciejewska-Paszek
Izabela
I
Pawłowska-Góral
Katarzyna
K
Aleksiewicz
Roman
R
Wardas
Maria
M
D020155:3-Hydroxybutyric Acid / Q000097:blood*; D000090:Acetoacetates / Q000097:blood*; D000208:Acute Disease; D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000633:toxicity; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D007213:Indomethacin / Q000008:administration & dosage / Q000627:therapeutic use; D007657:Ketone Bodies / Q000097:blood; D008106:Liver Cirrhosis, Experimental / Q000097:blood* / Q000517:prevention & control*; D008297:Male; D016595:Misoprostol / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits
D000090:Acetoacetates; D007657:Ketone Bodies; D016595:Misoprostol; C016635:acetoacetic acid; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D020155:3-Hydroxybutyric Acid; D007213:Indomethacin