Association of cytolethal distending toxin locus cdtB with enteropathogenic Escherichia coli isolated from patients with acute diarrhea in Calcutta, India.
Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These isolates were identified as being enteropathogenic E. coli (EPEC). In a retrospective study using a probe hybridization assay, 6 of 138 EPEC strains were found to harbor the cdtB locus. cdtB-positive isolates mostly belong to the O86a and O127a serogroups, with the former being associated with higher expression of CDT. Pulsed-field gel electrophoresis profiles showed that the EPEC strains harboring cdtB strains are genetically diverse.
Pandey
Mritunjay
M
National Institute of Cholera and Enteric Diseases, Indian Veterinary Research Institute, Calcutta, India.
Khan
Asis
A
Das
Suresh C
SC
Sarkar
Bhaswati
B
Kahali
Soumen
S
Chakraborty
Subhra
S
Chattopadhyay
Santanu
S
Yamasaki
Shinji
S
Takeda
Yoshifumi
Y
Nair
G Balakrish
GB
Ramamurthy
T
T
D000208:Acute Disease; D001427:Bacterial Toxins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D017931:DNA Primers; D003681:Dehydration / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000097:blood / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D009780:Occult Blood; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012703:Serotyping / Q000379:methods; D014839:Vomiting / Q000382:microbiology
D001427:Bacterial Toxins; D017931:DNA Primers; D029968:Escherichia coli Proteins; C067044:cytolethal distending toxin