10q25.3 (DMBT1) copy number changes in astrocytoma grades II and IV.
In the literature, it has been suggested that loss of the 10q25-26 region, including the DMBT1 gene (10q25.3), is correlated with initiation and/or malignant progression of astrocytomas, although the results of the studies on the loss of heterozygosity that led to this assumption are not unequivocal. For this reason, using double-target fluorescence in situ hybridization, we compared copy number changes of 10q25.3 to those of the pericentromeric region (10q12) in 10 cases each of astrocytoma grades II and IV. The same specimens were analyzed for copy number changes of chromosome 1, as a marker for polyploidy, and chromosome 7, which is often gained in astrocytomas of all grades. Our results show that selective loss of the 10q25.3 region was present in 2 of 10 specimens in both astrocytoma grade II and grade IV, occurring only in tumors with polysomy for 10q12. Furthermore, astrocytoma grade II often showed polyploidy for chromosomes 1, 7, and 10 (8 of 10 specimens). In addition, astrocytoma grade IV frequently exhibited losses of chromosome 10 in a high percentage of nuclei. Although based on a small number of cases, the results clearly show that loss of the 10q25.3 region is uncommon in astrocytoma grade II and mostly coincident with loss of chromosome 10 in grade IV tumors. These data indicate that selective loss of the 10q25.3 region, including the DMBT1 gene, is not an initiating event in the genesis of astrocytoma grade II.
Wessels
Peter H
PH
Research Institute Growth and Development (GROW), Maastricht, The Netherlands. PWES@SNEU.AZM.NL
Twijnstra
Albert
A
Kubat
Bela
B
Ummelen
Monique I J
MI
Claessen
Sandra M H
SM
Sciot
Raf
R
Merlo
Adrian
A
Ramaekers
Frans C S
FC
Speel
Ernst J M
EJ
Hopman
Anton H N
AH
D000328:Adult; D000368:Aged; D000373:Agglutinins; D001254:Astrocytoma / Q000737:chemistry / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D002135:Calcium-Binding Proteins; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D004268:DNA-Binding Proteins; D005260:Female; D018628:Gene Dosage; D005909:Glioblastoma / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins