title: Expression of gicerin enhances the invasive and metastatic activities of a mouse mammary carcinoma cell line.
abstract: Gicerin, an immunoglobulin superfamily protein, is expressed abundantly in the embryonic tissues and plays an important role in development through its cell adhesive activity. Interestingly, re-expression of gicerin is found in a variety of tumors. In the present study, the possible role of gicerin in the progression of mammary carcinoma was investigated. The normal mammary glands of mice were negative for gicerin, but sporadic mammary carcinoma cells expressed gicerin strongly on their surface. A mouse mammary carcinoma cell line, JYG-B, which is gicerin-negative was employed for introducing gicerin cDNA and the resultant gicerin transfectants were subsequently analyzed. In vitro, self-aggregation activity of the gicerin-transfectants progressed. For an in vivo study, invasive and metastatic potential of the cells was examined by a subcutaneous implantation into nude mice. The invasion of gicerin transfectants into the surrounding tissue was enhanced and severe metastasis to the lungs occurred. These findings suggest that gicerin is an effector for the malignant progression of mammary carcinoma.
authors: Department of Veterinary Anatomy, Graduate School of Agricultural and Biological Sciences, Osaka Prefecture University, Osaka 599-8531, Japan. ytsuka@jyui.vet.osakafu-u.ac.jp
authors: Yasuhiro
authors: Y
authors: Tsukamoto
authors: Takashi
authors: T
authors: Sakaiuchi
authors: Satoshi
authors: S
authors: Hiroi
authors: Masaru
authors: M
authors: Furuya
authors: Seiichiro
authors: S
authors: Tsuchiya
authors: Fumihiko
authors: F
authors: Sasaki
authors: Naomasa
authors: N
authors: Miki
authors: Eiichi
authors: E
authors: Taira
mesh_terms: D000818:Animals; D030161:Avian Proteins; D015153:Blotting, Western; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology; D008321:Mammary Glands, Animal / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D013997:Time Factors; D014162:Transfection
chemical_list: D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C497903:Mcam protein, mouse; D009419:Nerve Tissue Proteins