Synthesis of 13C-labeled iodoacetanilide and application to quantitative peptide analysis by isotope differential mass spectrometry.
13C-Labeled and unlabeled iodoacetanilides have been synthesized for covalent modification of the sulfhydryl groups of cysteine residues in proteins or peptides. A combination of these reagents, coupled with mass spectrometry, is a powerful tool for quantitative analysis of peptides and hence proteins.
Niwayama
Satomi
S
Department of Chemistry, Oklahoma State University, Stillwater 74078-3071, USA. niwayama@biochem.okstate.edu
Kurono
Sadamu
S
Matsumoto
Hiroyuki
H
D000083:Acetanilides / Q000138:chemical synthesis* / Q000737:chemistry; D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D003545:Cysteine / Q000737:chemistry; D006847:Hydrocarbons, Iodinated / Q000138:chemical synthesis* / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000083:Acetanilides; D002247:Carbon Isotopes; D006847:Hydrocarbons, Iodinated; D010455:Peptides; D013438:Sulfhydryl Compounds; D003545:Cysteine