Norepinephrine transporter splice variants and their interaction with substrates and blockers.
Norepinephrine transporter (NET), a member of the Na+/Cl--dependent neurotransmitter transporter family, displays species-specific isoforms produced by alternative RNA splicing. This occurs at 3'-flanking coding and noncoding regions, resulting in different carboxy-terminals. When these NET splice variants were expressed in cultured cell lines, the characteristics of substrate transport and the sensitivity to uptake inhibitors differed between isoforms. The different functional expression suggests the physiological importance of the action and interaction of NET splice variants in synaptic transmission.
Kitayama
Shigeo
S
Department of Dental Pharmacology, Okayama University, Graduate School of Medicine and Dentistry, Shikata 2-5-1, Okayama 700-8525, Japan. shigeok@md.okayama-u.ac.jp
Dohi
Toshihiro
T
D017398:Alternative Splicing; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation / Q000502:physiology*; D006801:Humans; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027981:Symporters / Q000235:genetics* / Q000378:metabolism*
D050484:Norepinephrine Plasma Membrane Transport Proteins; C493227:SLC6A2 protein, human; D027981:Symporters