Comparison between oleic acid and docosahexaenoic acid binding to interphotoreceptor retinoid-binding protein.
Interphotoreceptor retinoid-binding protein (IRBP), which binds retinoids and fatty acids, is the major soluble protein in the interphotoreceptor matrix (IPM) but its role remains ambiguous. Using competitive fluorescence and tryptophan-quenching assays we found oleic acid and other cis-monounsaturated fatty acids bind much more strongly than does docosahexaenoic acid to bovine IRBP. IPM's fatty acid composition was determined: it was richer in oleic acid than either the retinal pigment epithelium or rod outer segments. This may imply oleic acid has a key role in the balance and transport of retinoids and fatty acids in the retina.
Semenova
Ekaterina M
EM
Department of Pharmaceutical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, Scotland, UK.
Converse
Carolyn A
CA
D000818:Animals; D002417:Cattle; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005136:Eye Proteins; D005227:Fatty Acids / Q000032:analysis; D005453:Fluorescence; D008024:Ligands; D019301:Oleic Acid / Q000378:metabolism*; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism; D000099214:Retinol-Binding Proteins, Interstitial
D004281:Docosahexaenoic Acids; D005136:Eye Proteins; D005227:Fatty Acids; D008024:Ligands; D019301:Oleic Acid; D012177:Retinol-Binding Proteins; D014801:Vitamin A; D000099214:Retinol-Binding Proteins, Interstitial