Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol.
3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
Kauffman
Mary E
ME
Idaho National Engineering and Environmental Laboratory, PO Box 1625, MS-2203, Idaho Falls, ID 83415, USA.
Keener
William K
WK
Clingenpeel
Scott R
SR
Watwood
Mary E
ME
Reed
David W
DW
Fujita
Yoshiko
Y
Lehman
R Michael
RM
D000114:Acetylene / Q000031:analogs & derivatives* / Q000378:metabolism; D016956:Burkholderia cepacia / Q000201:enzymology / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D043367:Ralstonia / Q000201:enzymology / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D002396:Catechols; D005456:Fluorescent Dyes; C031136:3-methylcatechol; D014050:Toluene; D000114:Acetylene