title: Quantifying adenovirus-neutralizing antibodies by luciferase transgene detection: addressing preexisting immunity to vaccine and gene therapy vectors.
abstract: The presence of various levels of anti-adenovirus serotype 5 (Ad5)-neutralizing antibodies in humans is thought to contribute to the inconsistent clinical results obtained so far in diverse gene transfer and vaccination studies and might preclude universal dosing with recombinant Ad5. Prescreening of individuals eligible for Ad5 or alternative serotype treatment and subsequently tailoring the vector dose might aid in ensuring the consistency of clinical parameters. For this purpose, a qualified Ad neutralization assay is required. Here we have tested the different protocols used to date to determine anti-Ad neutralizing activity. Based on simplicity, speed, high throughput, sensitivity, and robustness, we propose a qualified assay in which Ad neutralization is monitored by luciferase reporter gene expression.
authors: Vaccine R&D Division, Crucell Holland BV, 2301 CA Leiden, The Netherlands.
authors: Mieke C
authors: MC
authors: Sprangers
authors: Wandena
authors: W
authors: Lakhai
authors: Wouter
authors: W
authors: Koudstaal
authors: Marielle
authors: M
authors: Verhoeven
authors: Björn F
authors: BF
authors: Koel
authors: Ronald
authors: R
authors: Vogels
authors: Jaap
authors: J
authors: Goudsmit
authors: Menzo J E
authors: MJ
authors: Havenga
authors: Stefan
authors: S
authors: Kostense
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D016679:Genome, Viral; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008156:Luciferases / Q000032:analysis / Q000235:genetics*; D009500:Neutralization Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D007074:Immunoglobulin G; D008156:Luciferases