16S rDNA targeted PCR for the detection of Paenibacillus macerans.
OBJECTIVE
To develop a PCR detection method, which could be used for the detection of Paenibacillus macerans in environmental samples or to help the identification of strains suspected to belong to this species.


RESULTS
Primers specific for P. macerans were developed based on the 16S rRNA gene sequence and were evaluated by PCR performed with genomic DNA from other Paenibacillus, other bacteria and DNA from soil as templates. The primers were shown to be specific for P. macerans strains and to amplify a 981-bp amplicon. Vegetative cells of P. macerans LMD 24.10T were tracked in Cerrado soil in 24-h experiments and PCR allowed the detection of 103 introduced cells per gram of dry soil.


CONCLUSIONS
This PCR detection method was adequate to assess the presence of P. macerans in Cerrado soil.


CONCLUSIONS
It can also be used after culturing to rapid confirm the identity of isolates suspected to belong to P. macerans.
Vollú
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Laboratório de Genética Microbiana, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
dos Santos
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SC
Seldin
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D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S