title: Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis.
abstract: The precise mechanistic relationship between gene activation and repression events is a central question in mammalian organogenesis, as exemplified by the evolutionarily conserved sine oculis (Six), eyes absent (Eya) and dachshund (Dach) network of genetically interacting proteins. Here, we report that Six1 is required for the development of murine kidney, muscle and inner ear, and that it exhibits synergistic genetic interactions with Eya factors. We demonstrate that the Eya family has a protein phosphatase function, and that its enzymatic activity is required for regulating genes encoding growth control and signalling molecules, modulating precursor cell proliferation. The phosphatase function of Eya switches the function of Six1-Dach from repression to activation, causing transcriptional activation through recruitment of co-activators. The gene-specific recruitment of a co-activator with intrinsic phosphatase activity provides a molecular mechanism for activation of specific gene targets, including those regulating precursor cell proliferation and survival in mammalian organogenesis.
authors: Howard Hughes Medical Institute, School and Department of Medicine, UCSD, 9500 Gilman Drive, Room 345, La Jolla, California 92093-0648, USA. seli@ucsd.edu
authors: Xue
authors: X
authors: Li
authors: Kenneth A
authors: KA
authors: Oghi
authors: Jie
authors: J
authors: Zhang
authors: Anna
authors: A
authors: Krones
authors: Kevin T
authors: KT
authors: Bush
authors: Christopher K
authors: CK
authors: Glass
authors: Sanjay K
authors: SK
authors: Nigam
authors: Aneel K
authors: AK
authors: Aggarwal
authors: Richard
authors: R
authors: Maas
authors: David W
authors: DW
authors: Rose
authors: Michael G
authors: MG
authors: Rosenfeld
mesh_terms: D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D004423:Ear / Q000196:embryology; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D038081:Organogenesis; D010902:Pituitary Gland / Q000196:embryology / Q000378:metabolism; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C466082:Eya3 protein, mouse; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C490947:Six1 protein, mouse; D015534:Trans-Activators; C103968:dac protein, Drosophila; C501156:Eya1 protein, mouse; D017027:Protein Tyrosine Phosphatases