A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates.
A real-time PCR genotypic assay was developed for the detection of isoniazid (INH) resistance in Mycobacterium tuberculosis. The assay detects mutations C(-15)T and, possibly, G(-24)T in the regulatory region of the inhA gene and proved as sensitive and specific as nucleotide sequencing in all the clinical isolates tested. Our assays mapped the mutations efficiently in 10 out of 35 resistant isolates, thereby covering 29% of all resistant strains.
Rindi
Laura
L
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, Via San Zeno 35/39, I-56127 Pisa, Italy. rindi@biomed.unipi.it
Bianchi
Lea
L
Tortoli
Enrico
E
Lari
Nicoletta
N
Bonanni
Daniela
D
Garzelli
Carlo
C
D000995:Antitubercular Agents / Q000494:pharmacology*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D017354:Point Mutation / Q000187:drug effects / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D000995:Antitubercular Agents; D007538:Isoniazid