[Effect of dahuang zhechong pill on transforming growth factor-beta 1 in hepatic stellate cells in rats].
OBJECTIVE
To explore the effect of Dahuang Zhechong Pill (DHZCP) on transforming growth factor-beta 1 (TGF-beta 1) in rats hepatic stellate cells.


METHODS
Liver fibrosis model rats were induced by CCl4 compound factor, and treated with DHZCP in three different dosages (ordinary, double and triple) separately. TGF-beta 1 and alpha-smooth muscle actin (alpha-SMA) synthesis expression, and grades of fibrosis were observed.


RESULTS
TGF-beta 1 and alpha-SMA expression in the group treated with ordinary dose of DHZCP was insignificantly different from those in the control group (P > 0.05), but the expression attenuated significantly after treatment with double or triple dose of DHZCP in the portal area and discontinuous fibrous septum (P < 0.05, P < 0.01).


CONCLUSIONS
DHZCP of larger dosage could inhibit the hepatic stellate cells proliferation and secretion of TGF-beta 1, and reduce the genesis of collagen, so as to weaken the auto-secretion amplifying response of the cells, which might be one of the chief mechanisms of DHZCP in antagonizing liver fibrosis.
Li
Lansheng
L
Department of Chinese Medicine, Zhuhai Municipal People's Hospital, 3rd Affiliated Hospital of Medical College of Jinan University, Guangdong 519000. lilansheng@21cn.com
Xu
Man
M
Liu
Zhi-long
ZL
D000199:Actins; D000818:Animals; D002252:Carbon Tetrachloride Poisoning; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005109:Extracellular Matrix; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D051381:Rats; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
D000199:Actins; D004365:Drugs, Chinese Herbal; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1