Silanized surfaces for in vitro studies of actomyosin function and nanotechnology applications.
We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to find a substitute for the polymer resists. We have found that surfaces derivatized with trimethylchlorosilane (TMCS) were superior to all other surfaces tested, including nitrocellulose. High-quality actin filament motility was observed up to 6 days after incubation with HMM and the fraction of motile actin filaments and the velocity of smooth sliding were generally higher on TMCS than on nitrocellulose. The actomyosin function on TMCS-derivatized glass and nitrocellulose is considered in relation to roughness and hydrophobicity of these surfaces. The results suggest that TMCS is an ideal substitute for polymer resists in the nanostructured in vitro motility assay. Furthermore, TMCS derivatized glass also seems to offer several advantages over nitrocellulose for HMM adsorption in the ordinary in vitro motility assay.
Sundberg
Mark
M
Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden.
Rosengren
Jenny P
JP
Bunk
Richard
R
Lindahl
Joakim
J
Nicholls
Ian A
IA
Tågerud
Sven
S
Omling
Pär
P
Montelius
Lars
L
Månsson
Alf
A
D000205:Actomyosin / Q000502:physiology*; D000327:Adsorption; D000818:Animals; D003101:Collodion; D007700:Kinetics; D008722:Methods; D009068:Movement; D036103:Nanotechnology / Q000379:methods*; D011817:Rabbits; D012825:Silicon; D013499:Surface Properties; D014297:Trimethylsilyl Compounds
D014297:Trimethylsilyl Compounds; C039293:trimethylchlorosilane; D003101:Collodion; D000205:Actomyosin; D012825:Silicon