title: Neutrophil myeloperoxidase activity and the influence of two single-nucleotide promoter polymorphisms.
abstract: Myeloperoxidase (MPO) catalyses the formation of hypochlorous acid and is involved in many (patho)physiological processes. The present study was designed to determine the effect of two MPO promoter polymorphisms (463G/A and 129G/A) on enzyme activity. In 243 healthy controls, genotypes were determined and MPO activity was measured on a single-cell level using a haematological analyser. The 129G/A polymorphism reduces MPO activity in neutrophils, whereas for the 463G/A polymorphism, only gender-dependent differences in MPO activity in older age groups could be found. When studying these polymorphisms in disease, therefore, age and gender should be included in the analysis.
authors: Clinical and Experimental Immunology, University Hospital Maastricht, Maastricht, The Netherlands. b.rutgers@immuno.unimaas.nl
authors: Abraham
authors: A
authors: Rutgers
authors: Peter
authors: P
authors: Heeringa
authors: Joyce E H M
authors: JE
authors: Giesen
authors: Ruud T
authors: RT
authors: Theunissen
authors: Heinz
authors: H
authors: Jacobs
authors: Jan Willem Cohen
authors: JW
authors: Tervaert
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005783:Gender Identity; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D006997:Hypochlorous Acid; D009195:Peroxidase