title: The use of transgenic mice for the production of a human monoclonal antibody specific for human CD69 antigen.
abstract: The CD69 antigen is the earliest activation marker expressed on leukocyte surfaces after stimulation and it has been correlated with disease state in a variety of inflammatory and autoimmune diseases. We were interested in the generation of a human monoclonal antibody (mAb) against the CD69 antigen. To do this, mice carrying human Ig transgenes (on an inactivated endogenous immunoglobulin H and Igkappa background) were immunized with rat cells transfected with the human CD69 molecule. From over 2000 hybridoma clones generated in different fusions, we were able to obtain a human monoclonal antibody, hAIM-29, which specifically recognizes human CD69 on the surface of activated-human leukocytes. We demonstrate that the antibody is specific for the human CD69 molecule, as shown by double staining with mouse anti-human CD69 antibodies, ELISA, immunoblot and immunoprecipitation studies. Results of additional experiments show that hAIM-29 activates intracellular calcium influx without Ig cross-linking and enhances phorbol myristate acetate-induced cell proliferation in a manner similar to other mouse anti-CD69 antibodies. This report is the first to describe the isolation and characterization of a novel human mAb, hAIM-29, which may have therapeutic potential in diseases associated with the presence of activated cells expressing CD69 antigen.
authors: Area de Inmunología, Facultad de Ciencias, Universidad de Vigo, Lagoas-Marcosende s/n 36200 Vigo, Pontevedra, Spain.
authors: Ana
authors: A
authors: Molina
authors: Mónica
authors: M
authors: Valladares
authors: Susana
authors: S
authors: Magadán
authors: David
authors: D
authors: Sancho
authors: Fernando
authors: F
authors: Viedma
authors: Irene
authors: I
authors: Sanjuan
authors: Francisco
authors: F
authors: Gambón
authors: Francisco
authors: F
authors: Sánchez-Madrid
authors: Africa
authors: A
authors: González-Fernández
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D051381:Rats; D000099192:CD69 Antigens
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D002118:Calcium; D037181:Lectins, C-Type; D000099192:CD69 Antigens