Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection.
This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
Sousa-Nunes
Rita
R
Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom.
Rana
Amer Ahmed
AA
Kettleborough
Ross
R
Brickman
Joshua M
JM
Clements
Melanie
M
Forrest
Alistair
A
Grimmond
Sean
S
Avner
Philip
P
Smith
James C
JC
Dunwoodie
Sally L
SL
Beddington
Rosa S P
RS
D000818:Animals; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000096:biosynthesis; D004707:Endoderm / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015723:Gene Library; D051379:Mice / Q000196:embryology* / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D017422:Sequence Analysis, DNA / Q000379:methods
D018076:DNA, Complementary