Measurement of MxA mRNA or protein as a biomarker of IFNbeta bioactivity: detection of antibody-mediated decreased bioactivity (ADB).
Myxovirus A (MxA) is a protein that is specifically induced by treatment with type I cytokines and has proven to be a reliable biomarker of interferon-beta (IFNbeta) bioactivity. IFNbeta-induced MxA can be measured as either protein or mRNA in the blood of IFNbeta-treated patients with MS. In patients with MS who are treated with IFNbeta, loss of the MxA response is caused by high levels of anti-IFNbeta antibodies and is a sensitive marker of lost bioactivity.
Pachner
Andrew R
AR
Department of Neurology and Neurosciences, UMDNJ-New Jersey Medical School, Newark, NJ, USA.
Bertolotto
Antonio
A
Deisenhammer
Florian
F
D001682:Biological Availability; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001773:Blood Cells / Q000737:chemistry; D004351:Drug Resistance / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016899:Interferon-beta / Q000276:immunology* / Q000493:pharmacokinetics / Q000627:therapeutic use; D009103:Multiple Sclerosis / Q000097:blood / Q000188:drug therapy / Q000276:immunology; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger / Q000032:analysis*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D016899:Interferon-beta; D019204:GTP-Binding Proteins