title: Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice.
abstract: BACKGROUND
  Food allergy is a common disease without effective treatment. Since strict elimination of food allergens may be difficult, strategies for effective intervention are urgently needed.
  
  
  OBJECTIVE
  The aim was to investigate the prophylactic use of orally administrated FIP-fve, an immunomodulatory protein isolated from the edible mushroom Flammulina velutipes, in a murine model of food allergy.
  
  
  METHODS
  BALB/c mice were immunized twice intraperitoneally with ovalbumin (OVA), at an interval of 2 weeks. Before and during each period of immunization, FIP-fve (200 microg per mouse) or phosphate-buffered saline was given orally every other day with a total of five doses. Then OVA-specific antibodies and cytokine profiles were determined. Subsequently, the mice were orally challenged with OVA. Symptoms of anaphylaxis, levels of plasma histamine, and histology of intestines were examined.
  
  
  RESULTS
  Mice receiving oral FIP-fve treatment during sensitization to OVA had an impaired OVA-specific IgE response with a Th1-predominant cytokine profile. These mice were protected from systemic anaphylaxis-like symptoms induced by subsequent oral challenge with OVA.
  
  
  CONCLUSIONS
  Oral administration of FIP-fve has a Th1-skewing effect on the development of the allergen-specific immune response, and may serve the purpose of immunoprophylaxis for food allergy and other allergic diseases.
authors: Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: K Y
authors: KY
authors: Hsieh
authors: C I
authors: CI
authors: Hsu
authors: J Y
authors: JY
authors: Lin
authors: C C
authors: CC
authors: Tsai
authors: R H
authors: RH
authors: Lin
mesh_terms: D000284:Administration, Oral; D000707:Anaphylaxis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005656:Fungal Proteins / Q000276:immunology / Q000627:therapeutic use*; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007583:Jejunum / Q000473:pathology; D037102:Lectins / Q000276:immunology / Q000627:therapeutic use*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D018417:Th1 Cells / Q000276:immunology
chemical_list: D005656:Fungal Proteins; D037102:Lectins; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine; D007371:Interferon-gamma; D010047:Ovalbumin