title: Reactive oxygen species affect photomorphogenesis in Neurospora crassa.
abstract: In Neurospora crassa, several biological phenomena such as the synthesis of carotenoids in the mycelia and polarity of perithecia are regulated by light. We found that a sod-1 mutant, with a defective Cu,Zn-type superoxide dismutase (SOD), showed accelerated light-dependent induction of carotenoid accumulation in the mycelia compared with the wild type. The initial rate of light-induced carotenoid accumulation in the sod-1 mutant was faster than that in the vvd mutant known to accumulate high concentrations. This acceleration was suppressed by treatment with antioxidant reagents. Light-induced transcription of genes involved in carotenoid synthesis, al-1, -2, and -3, was sustained in the sod-1 mutant, whereas it was transient in the wild type. Moreover sod-1 was defective in terms of light-induced polarity of perithecia. By genetic analysis, the enhancement in light-inducible carotenoid synthesis in sod-1 was dependent on the wild type alleles of wc-1 and wc-2. However, the sod-1;vvd double mutant showed additive effects on the carotenoid accumulation in the mycelia. These results suggested that intracellular reactive oxygen species regulated by SOD-1 could affect the light-signal transduction pathway via WC proteins.
authors: Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244-0813, Japan.
authors: Yusuke
authors: Y
authors: Yoshida
authors: Kohji
authors: K
authors: Hasunuma
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015152:Blotting, Northern; D002338:Carotenoids / Q000378:metabolism; D008027:Light; D009154:Mutation; D009492:Neurospora crassa / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002338:Carotenoids; D013482:Superoxide Dismutase