title: Dependence of leukemic cell autofluorescence patterns on the degree of differentiation.
abstract: The characterisation of leukemic cell autofluorescence during differentiation, induced by 12-O-tetradecanoylphorbol 13-acetate and all-trans retinoic acid, was performed by autofluorescence microspectroscopy and multispectral imaging autofluorescence microscopy. We have found that a dependence exists between the cell autofluorescence pattern and the degree of cell differentiation. When cells differentiate, their autofluorescence emission changes, following the morphological and functional rearrangement of cell structures. A decrease in emission intensity and a different distribution of endogenous fluorophores are observed. Thus, autofluorescence monitoring on living cells is a potentially useful tool for in vitro study of the differentiation processes. Furthermore, different maturation steps can be distinguished on the basis of the cell fluorescence pattern, leading the way to future application of the technique in diagnostics.
authors: Centre of Excellence in Optronics, Largo Enrico Fermi, 6, 50125 Florence, Italy. monici@ino.it
authors: Monica
authors: M
authors: Monici
authors: Giovanni
authors: G
authors: Agati
authors: Franco
authors: F
authors: Fusi
authors: Riccardo
authors: R
authors: Pratesi
authors: Milena
authors: M
authors: Paglierani
authors: Valeria
authors: V
authors: Santini
authors: Pietro Antonio
authors: PA
authors: Bernabei
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D005453:Fluorescence; D018922:HL-60 Cells; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology*; D008856:Microscopy, Fluorescence; D018462:Photobiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine