Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs.
PMWS is a new emerging disease in swine herds worldwide. Field isolates of PCV-2, a putative major causative agent of PMWS, were isolated and genetically characterized. Viral genome of two field isolates (PC201DJ and PC201SS) from pigs showing typical PMWS was sequenced. The nucleotide sequence homology with other PCV-2 isolates was ranging from 95% to 99% in complete viral genomic sequence. The highly conserved nonanucleotide motif of replication origin was identical to that of other PCV-2 isolates. To determine the genetic heterogeneity of PCV-2 isolates, the phylogenetic tree based on the complete genome of PCV-2 isolates were constructed. Two PCV-2 field isolates were closely related to Canadian isolates of PCV-2. PCV-2 isolated from field may have an origin of North America and is possibly originated from importation of breeding stocks. The result indicates that although the genome of PCV-2 is relatively stable in general, minor genetic variations exist among PCV-2 isolates from the different geographic locations. These differences of viral genome might have an important implication for genetic characteristics of PCV-2 infection. Three major immunorelevant epitopes of capsid protein showed variations in amino acid sequences. Also, the variance of amino acid sequence in antigenic epitope existed between two Korean PCV-2 isolates.
Kim
Jin H
JH
Immunopathology laboratory, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Korea.
Lyoo
Young S
YS
D000818:Animals; D001483:Base Sequence; D018173:Circoviridae Infections / Q000662:veterinary*; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D016679:Genome, Viral; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D019282:Wasting Syndrome / Q000662:veterinary* / Q000821:virology; D014886:Weaning
D017931:DNA Primers