title: A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions.
abstract: We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate. In addition to elevated throughput, the PicoTiterPlate based amplifications (PTPCR) can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 microL reaction volume for the entire PicoTiterPlate. The bulk PTPCR product can be recovered and assayed with real-time PCR, or discrete PTPCR products can be driven to solid supports, enabling downstream applications such as translation/transcription or sequencing.
authors: 454 Life Sciences, Branford, CT 06405, USA. jleamon@454.com
authors: John H
authors: JH
authors: Leamon
authors: William L
authors: WL
authors: Lee
authors: Karrie R
authors: KR
authors: Tartaro
authors: Janna R
authors: JR
authors: Lanza
authors: Gary J
authors: GJ
authors: Sarkis
authors: Alex D
authors: AD
authors: deWinter
authors: Jan
authors: J
authors: Berka
authors: Michael
authors: M
authors: Weiner
authors: Jonathan M
authors: JM
authors: Rothberg
authors: Kenton L
authors: KL
authors: Lohman
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers; D005336:Fiber Optic Technology; D008855:Microscopy, Electron, Scanning; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004247:DNA