N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced in vivo clastogenicity: protective effects of aqueous neem leaf extract.
We evaluated the modifying effects of aqueous neem leaf extract on the in vivo clastogenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a potent gastric carcinogen by quantitation of micronuclei and chromosomal aberrations in metaphase cells from the bone marrow of male Wistar rats. Intraperitoneal injection of MNNG (40 mg/kg body weight) induced a significant increase in the frequency of micronuclei and chromosomal aberrations. Pretreatment with aqueous neem leaf extract (100 mg/kg body weight) significantly reduced MNNG-induced increase in micronuclei and chromosomal aberrations. These results reveal the chemoprotective potential of aqueous neem leaf extract against the clastogenic effects of MNNG.
Arivazhagan
S
S
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Nagini
S
S
Santhiya
S T
ST
Ramesh
A
A
D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D015162:Micronucleus Tests; D009153:Mutagens / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D008769:Methylnitronitrosoguanidine