title: Human serum amyloid P component attenuates the bacterial lipopolysaccharide-induced increase in blood-brain barrier permeability in mice.
abstract: Endotoxin challenge leads to septic shock, multi-organ failure and death in mice. Permeability of the blood-brain barrier (BBB) is increased by endotoxemia. Serum amyloid P component (SAP) is a lipopolysaccharide (LPS)-binding protein that can modulate the host reactions during infections. It is controversial whether SAP can protect from LPS toxicity in vivo or not. We have tested the effect of human SAP on BBB permeability of Salmonella typhimurium LPS-injected mice. The animals showed signs of sickness behaviour including immobility, anorexia, and diarrhoea. Intraperitoneally administered LPS increased the BBB permeability for sodium fluorescein for about 4-fold, and for albumin for more than 2-fold in brain cortex. SAP, given intravenously, had no effect on basal BBB permeability for albumin, although it decreased sodium fluorescein extravasation to brain tissue. In LPS-treated mice, SAP administration alleviated the symptoms of septic shock, and significantly inhibited the enhanced BBB permeability for both tracers. Our data indicate that human SAP may counteract the toxic effects of LPS during septic shock.
authors: Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt 62, H-6726 Szeged, Hungary.
authors: Szilvia
authors: S
authors: Veszelka
authors: Zoltán
authors: Z
authors: Urbányi
authors: Tamás
authors: T
authors: Pázmány
authors: László
authors: L
authors: Németh
authors: Izabella
authors: I
authors: Obál
authors: Ngo Thi Khue
authors: NT
authors: Dung
authors: Csongor S
authors: CS
authors: Abrahám
authors: Gábor
authors: G
authors: Szabó
authors: Mária A
authors: MA
authors: Deli
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D000683:Serum Amyloid P-Component / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D000683:Serum Amyloid P-Component