[Photochemical transformation of lactate dehydrogenase isoforms: UV-sensitivity in the presence of biogenous amines].
It has been all round investigation of UV-irradiation influence in a wide dose range on the structural and functional properties of human blood lactate dehydrogenase (LDH) isoenzymes. The photoprotective action of biogenous amines on the functional activity of different enzyme isoforms was found. It has been established that the protective action of biogenous amines is caused by the formation of complex LDH--biogenous amine and by the acception of the active oxygen forms by the molecules of lactate dehydrogenase. Under the conditions of exogenous singlet oxygen generation in the presence of methylene blue, the inactivation of immobilized LDH tetramers and subunits was observed, that shows participation of this active intermediate in the processes of UV-modification of the enzyme in soluble and immobilized states. The scheme of processes of LDH molecules phototransformations in the presence of biogenous amines has been suggested.
Artiukhov
V G
VG
Voronezh State University, Voronezh, 394006 Russia.
Nakvasina
M A
MA
Agisheva
N V
NV
D001679:Biogenic Amines / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D004800:Enzymes, Immobilized / Q000528:radiation effects; D004912:Erythrocytes / Q000201:enzymology* / Q000528:radiation effects; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays
D001679:Biogenic Amines; D004800:Enzymes, Immobilized; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D007770:L-Lactate Dehydrogenase