title: Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum.
abstract: The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel beta-strands that are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2-3 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein. The structural and surface resemblance to OB-fold family of proteins and the presence of highly conserved basic residues suggest that S28E may bind to RNA. A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA.
authors: Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada.
authors: Bin
authors: B
authors: Wu
authors: Adelinda
authors: A
authors: Yee
authors: Antonio
authors: A
authors: Pineda-Lucena
authors: Anthony
authors: A
authors: Semesi
authors: Theresa A
authors: TA
authors: Ramelot
authors: John R
authors: JR
authors: Cort
authors: Jin-Won
authors: JW
authors: Jung
authors: Aled
authors: A
authors: Edwards
authors: Weontae
authors: W
authors: Lee
authors: Michael
authors: M
authors: Kennedy
authors: Cheryl H
authors: CH
authors: Arrowsmith
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D017014:Methanobacterium / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins