title: Comparison of UV and tandem mass spectrometric detection for the high-performance liquid chromatographic determination of diclofenac in microdialysis samples.
abstract: High-performance liquid chromatography (HPLC) was used to analyze microdialysis samples obtained in vivo from human subcutaneous adipose tissue after topical application of the nonsteroidal anti-inflammatory drug diclofenac. For the reliable determination of diclofenac two different detection principles were applied in two different laboratories. One HPLC method utilized UV-detection at 280 nm, the other one used selected reaction monitoring mass spectrometry (MS). The HPLC-UV and -MS methods offered low limits of quantification of 10 and 1 ng/ml and an accuracy between 94.0-126.7 and 89.3-110.9%, respectively. However, a comparison showed that the HPLC-UV method failed to determine diclofenac in biological matrices, as both false negative and positive values were found. HPLC-MS is clearly superior to HPLC-UV due to a much more selective detection, increased sensitivity and shorter run times.
authors: Department of Clinical Pharmacology, Division of Clinical Pharmacokinetics, Vienna University School of Medicine, Währinger Gürtel 18-20, 1090 Vienna, Austria. bernhard.mayer@univie.ac.at
authors: Bernhard X
authors: BX
authors: Mayer
authors: Khodadad
authors: K
authors: Namiranian
authors: Pejman
authors: P
authors: Dehghanyar
authors: Reinhard
authors: R
authors: Stroh
authors: Hermann
authors: H
authors: Mascher
authors: Markus
authors: M
authors: Müller
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D016014:Linear Models; D017551:Microdialysis / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D004008:Diclofenac