BRPK, a novel protein kinase showing increased expression in mouse cancer cell lines with higher metastatic potential.
A novel protein kinase named BRPK was isolated and partially characterized. BRPK was expressed at a higher level in three carcinoma cell lines with higher metastatic potential. Mouse and human BRPK cDNAs are well conserved and encode 580 and 581 amino acids, respectively. BRPK has a serine/threonine-type protein kinase domain, and the recombinant proteins of BRPK were capable of autophosphorylation. The results of a comparative sequence analysis indicated a possible link of BRPK to BRAP2. BRAP2 is known to bind the nuclear localization signal of BRCA1. We cloned mouse BRAP2 cDNA and showed the presence of isoforms.
Nakajima
Akinori
A
Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry, Shikata-chou, Okayama 700-8558, Japan.
Kataoka
Ken
K
Hong
Mei
M
Sakaguchi
Masakiyo
M
Huh
Nam-ho
NH
D000595:Amino Acid Sequence; D000818:Animals; D019313:BRCA1 Protein / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D002460:Cell Line; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008545:Melanoma / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D014162:Transfection; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases
D019313:BRCA1 Protein; D002352:Carrier Proteins; D018076:DNA, Complementary; C111164:BRAP protein, human; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases