title: Cutting edge: gamma delta T cells provide help to B cells with altered clonotypes and are capable of inducing Ig gene hypermutation.
abstract: It has not been resolved whether gammadelta T cells can collaborate with germinal center B cells and support Ig hypermutation during an Ab response to a truly defined T-dependent Ag. In this study, we show that in the absence of alphabeta T cells, immunization with the well-defined T-dependent Ag, (4-hydroxy-3-nitrophenyl) acetyl (NP) conjugate, was able to induce Ig hypermutation. However, the clonotypes of B cells responding to NP were dramatically altered in TCR beta(-/-) mice. Unlike B cells in wild-type mice that use canonical VDJ rearrangements, most NP-responding B cells in mutant mice use analog genes of the J558 gene family. In addition, the majority of anti-NP Abs produced in mutant mice use kappaL chain instead of lambda1L chain, which dominates in mice of Igh(b) background. Thus, the B cell population that collaborates with gammadelta T cells is distinct from B cells interacting with conventional alphabeta Th cells.
authors: Department of Immunology, Baylor College of Medicine, Houston, TX 77030, USA. bzheng@bcm.tmc.edu
authors: Biao
authors: B
authors: Zheng
authors: Ekaterina
authors: E
authors: Marinova
authors: Jin
authors: J
authors: Han
authors: Tse-Hua
authors: TH
authors: Tan
authors: Shuhua
authors: S
authors: Han
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000008:administration & dosage / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002645:Chickens; D002999:Clone Cells; D005260:Female; D018858:Germinal Center / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology; D016130:Immunophenotyping; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009596:Nitrophenols / Q000008:administration & dosage / Q000276:immunology; D010648:Phenylacetates; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis / Q000502:physiology*; D027041:Somatic Hypermutation, Immunoglobulin; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D005719:gamma-Globulins / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D006241:Haptens; D009596:Nitrophenols; D010648:Phenylacetates; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D005719:gamma-Globulins; C026242:4-hydroxy-5-nitrophenyl acetic acid