Molecular cloning of a new lectin gene from Z. grandiflora.
Using RNA extracted from Zephyrathes grandiflora young leaves and primers designed according to the conservative regions of Amaryllidaceae lectins, the full-length cDNA of Z. grandiflora agglutinin (ZGA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of ZGA was 934 bp and contained a 576 bp open reading frame (ORF) encoding a 191 amino acid protein. Through comparative analysis of zga gene and its deduced amino acid sequence with those of other Amaryllidaceae species, it was found that zga encoded a precursor lectin with signal peptide. Molecular modeling suggested that ZGA was a mannose-binding lectin with three mannose-binding sites like lectins from other Amaryllidaceae species. Southern blot analysis of the genomic DNA revealed that zga belonged to a multi-copy gene family like those of many other Amaryllidaceae species such as Galanthus nivalis and Clivia miniata.
Kai
Guoyin
G
Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Zheng
Jingui
J
Zhang
Lei
L
Pang
Yongzhen
Y
Liao
Zhihua
Z
Li
Zhugang
Z
Zhao
Linxia
L
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D019668:Liliaceae / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D037121:Plant Lectins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D037121:Plant Lectins