A microsatellite marker based framework linkage map of Vitis vinifera L.
We have constructed a framework linkage map based on microsatellite markers for Vitis vinifera L., the European wine grape. The mapping population consisted of 153 progeny plants from a cross of Vitis vinifera cvs. Riesling x Cabernet Sauvignon. One hundred fifty-two microsatellite markers and one polymorphic EST marker have been mapped to 20 linkage groups (2 n=38). The map covers 1,728 cM with an average distance between markers of 11.0 cM. Estimates of genome size, expected genome coverage, and observed genome coverage were determined with 135-140 markers. Genome length estimates differed between paternal and maternal data sets. Observed approximate genome coverage was 65% versus an expected coverage of 90%. Meiotic recombination rates were not significantly different between maternal and paternal parents. This map has been adopted as a reference map for the International Grape Genome Program.
Riaz
S
S
Department of Viticulture and Enology, University of California, One Shields Avenue, Davis, CA 95616, USA.
Dangl
G S
GS
Edwards
K J
KJ
Meredith
C P
CP
D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D008540:Meiosis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D027843:Vitis / Q000235:genetics*
D005819:Genetic Markers