title: Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection.
abstract: OBJECTIVE
  To investigate the clonality of ectopic gland cells in peritoneal endometriosis.
  
  
  METHODS
  Prospective study. University hospital.
  
  
  METHODS
  Seventeen women with surgically diagnosed endometriosis.
  
  
  METHODS
  Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
  
  
  METHODS
  Clonality analysis used the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay after digestion of the DNA with methylation-sensitive endonuclease.
  
  
  RESULTS
  Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
  
  
  CONCLUSIONS
  The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.
authors: Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Miyagi, Japan. nabeakt@umin.ac.jp
authors: Hiroshi
authors: H
authors: Nabeshima
authors: Takashi
authors: T
authors: Murakami
authors: Kosuke
authors: K
authors: Yoshinaga
authors: Kazuyo
authors: K
authors: Sato
authors: Yukihiro
authors: Y
authors: Terada
authors: Kunihiro
authors: K
authors: Okamura
mesh_terms: D002999:Clone Cells / Q000473:pathology*; D019175:DNA Methylation; D004715:Endometriosis / Q000473:pathology*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D015907:Hysteroscopy; D053685:Laser Therapy; D042282:Microdissection; D010532:Peritoneal Diseases / Q000473:pathology*; D010735:Phosphoglycerate Kinase / Q000235:genetics; D011110:Polymorphism, Genetic; D011446:Prospective Studies
chemical_list: D010735:Phosphoglycerate Kinase