title: Water dispersible microbicidal cellulose acetate phthalate film.
abstract: BACKGROUND
  Cellulose acetate phthalate (CAP) has been used for several decades in the pharmaceutical industry for enteric film coating of oral tablets and capsules. Micronized CAP, available commercially as "Aquateric" and containing additional ingredients required for micronization, used for tablet coating from water dispersions, was shown to adsorb and inactivate the human immunodeficiency virus (HIV-1), herpesviruses (HSV) and other sexually transmitted disease (STD) pathogens. Earlier studies indicate that a gel formulation of micronized CAP has a potential as a topical microbicide for prevention of STDs including the acquired immunodeficiency syndrome (AIDS). The objective of endeavors described here was to develop a water dispersible CAP film amenable to inexpensive industrial mass production.
  
  
  METHODS
  CAP and hydroxypropyl cellulose (HPC) were dissolved in different organic solvent mixtures, poured into dishes, and the solvents evaporated. Graded quantities of a resulting selected film were mixed for 5 min at 37 degrees C with HIV-1, HSV and other STD pathogens, respectively. Residual infectivity of the treated viruses and bacteria was determined.
  
  
  RESULTS
  The prerequisites for producing CAP films which are soft, flexible and dispersible in water, resulting in smooth gels, are combining CAP with HPC (other cellulose derivatives are unsuitable), and casting from organic solvent mixtures containing approximately equal to 50 to approximately equal to 65% ethanol (EtOH). The films are approximately equal to 100 micron thick and have a textured surface with alternating protrusions and depressions revealed by scanning electron microscopy. The films, before complete conversion into a gel, rapidly inactivated HIV-1 and HSV and reduced the infectivity of non-viral STD pathogens >1,000-fold.
  
  
  CONCLUSIONS
  Soft pliable CAP-HPC composite films can be generated by casting from organic solvent mixtures containing EtOH. The films rapidly reduce the infectivity of several STD pathogens, including HIV-1. They are converted into gels and thus do not have to be removed following application and use. In addition to their potential as topical microbicides, the films have promise for mucosal delivery of pharmaceuticals other than CAP.
authors: Biochemical Virology Laboratory, The Lindsley F Kimball Research Institute of the New York Blood Center, New York, NY 10021, USA. arneurath@att.net
authors: A Robert
authors: AR
authors: Neurath
authors: Nathan
authors: N
authors: Strick
authors: Yun-Yao
authors: YY
authors: Li
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D015497:HIV-1 / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009040:Motion Pictures; D014867:Water / Q000737:chemistry
chemical_list: D019380:Anti-HIV Agents; D000890:Anti-Infective Agents; D014867:Water; D000431:Ethanol; D002482:Cellulose; C011708:cellulose acetate phthalate; C008079:hydroxypropylcellulose