Evaluation of myeloid and lymphoid dendritic cells in peritoneal fluid in women with non-malignant ovarian tumors.
OBJECTIVE
Identification of myeloid and lymphoid dendritic cells (DCs) in peritoneal fluid (PF) and peripheral blood (PB) of patients with ovarian pathology.


METHODS
PF and PB were collected from 60 patients who underwent laparoscopy because of non-malignant ovarian tumors. Mononuclear cells were separated by gradient centrifugation. The cell surface antigens were determined by flow cytometry using monoclonal antibodies.


RESULTS
Both myeloid and lymphoid DCs were detected in PF and PB of women with ovarian tumors. The percentage of myeloid DCs was significantly higher in PF than in PB. The concentration of PF myeloid DCs was the highest (P < 0.05) in patients with dermoid cysts (0.67 x 10(6)/mL PF) in comparison with the other studied groups, excluding patients with normal pelvis.


CONCLUSIONS
Domination of myeloid and not lymphoid cells in PF may support the hypothesis that local PF immune disturbances may play a role in some non-malignant ovarian pathology.
Wertel
Iwona
I
Department of Gynecology, University School of Medicine, ul. Staszica 16, 20-081 Lublin, Poland. Iwonawertel@wp.pl
Kotarski
Jan
J
Roliński
Jacek
J
Bojarska-Junak
Agnieszka
A
Gogacz
Marek
M
D000293:Adolescent; D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology*; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000276:immunology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000097:blood / Q000166:cytology* / Q000276:immunology; D022423:Myeloid Cells / Q000166:cytology* / Q000276:immunology; D010048:Ovarian Cysts / Q000276:immunology* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000473:pathology