Acute bile duct ligation ameliorates ischemic renal failure.
BACKGROUND
Biliary obstruction affects the renal response to ischemia and also elicits a hepatic cytokine response. Using a murine model, we now test the hypothesis that these hepatic cytokines help determine the outcome of ischemic acute renal failure.


METHODS
C3H/HEN mice were subjected to bile duct ligation 24 h (ABDL) or 7 days (CBDL) prior to induction of acute ischemic renal failure (ARF). Serum creatinine (Scr), cytokine mRNA abundance, and renal histology were studied 24 h after renal ischemia.


RESULTS
ABDL prior to ARF resulted in amelioration of renal injury (Scr 0.7 +/- 0.1 mg/dl compared to 2.5 +/- 0.1 mg/dl in sham/ARF group, (mean +/- SE, n = 11/group). CBDL exacerbated renal injury. Increased hepatic mRNA for interleukin-10 (IL10) and interleukin-1 receptor antagonist (IL1RA) was detected in the ABDL/ARF group but not in the CBDL/ARF group. These data suggest that hepatic production of IL10 and IL1RA in response to ABDL ameliorates ischemic ARF, an effect that is lost after several days of BDL.


CONCLUSIONS
Our data support the concept that hepatic cytokines modulate renal injury. This adds a new dimension in our understanding of renal injury in the setting of hepatic disease.
Jeyarajah
D Rohan
DR
Department of Surgery, Southwestern Medical School, Dallas, Tex 75390-9156, USA. rohan.jeyarajah@utsouthwestern.edu
Kielar
Mariusz L
ML
Zhou
Xin J
XJ
Zhang
Ying
Y
Lu
Christopher Y
CY
D058186:Acute Kidney Injury / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D002779:Cholestasis / Q000503:physiopathology; D003135:Common Bile Duct / Q000503:physiopathology / Q000601:surgery*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D007511:Ischemia / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply*; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000517:prevention & control*; D008026:Ligation; D008099:Liver / Q000737:chemistry / Q000503:physiopathology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000378:metabolism; D018123:Receptors, Interleukin / Q000037:antagonists & inhibitors / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D053701:Receptors, Interleukin-10
D012333:RNA, Messenger; D018123:Receptors, Interleukin; D017472:Receptors, Interleukin-1; D053701:Receptors, Interleukin-10; D003404:Creatinine