Single-cell analysis of lambda immunity regulation.
We have examined expression of the lambdacI operon in single cells via a rex Colon, two colons gfp substitution. Although average fluorescence agreed with expectations for expression of lambda-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.
Baek
Kristoffer
K
Department of Molecular Cell Biology, University of Copenhagen, Øster Farimagsgade 2A, DK-1353, K, Copenhagen, Denmark.
Svenningsen
Sine
S
Eisen
Harvey
H
Sneppen
Kim
K
Brown
Stanley
S
D019677:Artificial Gene Fusion; D010582:Bacteriophage lambda / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured / Q000528:radiation effects / Q000648:ultrastructure; D003090:Coliphages; D004260:DNA Repair; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins; D007111:Immunity, Cellular; D008164:Luminescent Proteins / Q000378:metabolism; D008242:Lysogeny; D009876:Operon; D010957:Plasmids; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins