title: Desialylated N-CAM and chromatin-derived acidic peptide effects in the hypothalamus.
abstract: Chromatin-derived acidic peptides (ACPs) have been shown to acutely modulate hypothalamic catecholamine release. To investigate whether this effect is mediated through membrane polysialylated neural-cell adhesion molecule (PSA-N-CAM), we pretreated rat hypothalamic synaptosomes with neuraminidase enzyme, which partially cleaves sialic acid residues from N-CAM, and perfused them with ACP-1 (Asp-Asp-Ser-Asp-Glu-Glu-Asn) or a more lipophilic derivative, ACP-2 ([Ala-Ile-Ser-Pro]-Asp-Asp-Ser-Asp-Glu-Glu-Asn). We have found that neuraminidase completely abolish the inhibitory effect of ACP-1 on dopamine release, while the inhibitory activity of ACP-1 on norepinephrine release is partially lost. On the other hand, ACP-2 inhibition of dopamine release is not modified by neuraminidase pretreatment.
authors: Department of Drug Sciences, School of Pharmacy, G. D'Annunzio University, via dei Vestini, 66013, Chieti, Italy.
authors: Chiara Di
authors: CD
authors: Nisio
authors: Luigi
authors: L
authors: Brunetti
authors: Diana L
authors: DL
authors: Esposito
authors: Lucia
authors: L
authors: Recinella
authors: Giustino
authors: G
authors: Orlando
authors: Barbara
authors: B
authors: Michelotto
authors: Michele
authors: M
authors: Vacca
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D002843:Chromatin; D019006:Neural Cell Adhesion Molecules; D010455:Peptides; D009439:Neuraminidase