[Effects of quercetin on platelet-endothelial cell adhesion and their expression of adhesion molecules].
OBJECTIVE
To observe the effect of quercetin (Que) on the adhesion of platelets to cultured endothelial cells and adhesion molecule expression by human umbilical vein endothelial cells (HUVEC) and platelets.


METHODS
[3H]-Adenine labeled platelets were incubated with HUVEC to investigate the effect of Que on adhesion of platelets to HUVEC. The number of platelets adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy. TNF-alpha induced HUVEC expression ICAM-1 and thrombin induced platelets expression of P-selectin were measured by flow cytometry.


RESULTS
Que (0.3-2.4 mumol.L-1) was shown to inhibit the increase of P-selectin expression of thrombin activated platelets. Pretreatment of HUVEC with tumor necrosis factor (TNF-alpha) significantly increased platelets adhesion to HUVEC and the expression ICAM-1. Que (0.6-2.4 mumol.L-1) inhibited this effect of TNF-alpha in a concentration-dependent manner.


CONCLUSIONS
Que can inhibit the adhesion of platelets to HUVEC and the expression of adhesion molecules (P-selectin and ICAM-1).
Liang
Zhong-qin
ZQ
Department of Pharmacology, Suzhou University Medical College, Suzhou, 215007 China. Liangzhq@etang.com
Zhu
Yi
Y
Gu
Zhen-lun
ZL
Lu
Qun
Q
Fan
Pan-sheng
PS
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1; D011794:Quercetin