Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1,2-epoxyoctane.
Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilised biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M were utilised for bead preparation.
Maritz
J
J
Department of Pharmaceutical Chemistry, Potchefstroom University for CHE, Private Bag X6001, 2520, Potchefstroom, South Africa.
Krieg
H M
HM
Yeates
C A
CA
Botes
A L
AL
Breytenbach
J C
JC
D000464:Alginates; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000502:physiology; D004789:Enzyme Activation; D004795:Enzyme Stability; D004851:Epoxide Hydrolases / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology*; D020723:Glucuronic Acid / Q000502:physiology*; D006603:Hexuronic Acids; D007700:Kinetics; D008863:Microspheres; D009795:Octanes / Q000378:metabolism*; D013237:Stereoisomerism
C487864:1,2-epoxyoctane; D000464:Alginates; D004852:Epoxy Compounds; D006603:Hexuronic Acids; D009795:Octanes; D020723:Glucuronic Acid; D004851:Epoxide Hydrolases