Method for patterning stretched DNA molecules on mica surfaces by soft lithography.
Lambda DNA was stretched and patterned on mica surface using soft lithography. A highly diluted solution of amino propyl trimethoxy silane in hexane was deposited on a line patterned polydimethylsiloxane (PDMS) stamp. The functionalized stamp was then used to pick up DNA by molecular combing while the line patterns are parallel to the liquid surface. The stamp was then microcontact printed on freshly cleaved mica. We successfully obtained stretched DNA pattern on mica surface. DNA was found to be stretched in patterns perpendicular to those carved on the stamp. The stretched DNA population was large enough to be used for molecular biology mapping studies. Furthermore, the possibility of locating stretched DNA molecules in the desired position by stamping makes this method a good candidate for assembling non-semiconductor molecular devices.
Gad
M
M
Food Engineering Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. gmuhamme@affrc.go.jp
Sugiyama
Shigeru
S
Ohtani
Toshio
T
D000538:Aluminum Silicates; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
D000538:Aluminum Silicates; D004129:Dimethylpolysiloxanes; D009841:Oligonucleotides; D012828:Silicones; C013830:baysilon; D004247:DNA; C011934:mica