Use of ribosomal DNA sequence data to characterize and detect a neogregarine pathogen of Solenopsis invicta (Hymenoptera: Formicidae).
A neogregarine parasite of the red imported fire ant, Solenopsis invicta, was discovered recently in Florida and tentatively placed in the Mattesia genus based on morphological characterization. S. invicta infected with this Mattesia species exhibited a characteristic yellowing of the cuticle which was designated Mattesia "yellow-head disease" (YHD). The 18S rRNA gene sequence from Mattesia YHD was elucidated and compared with the neogregarine pathogens, Mattesia geminata and Ophriocystis elektroscirrha. The sequence data support the previous conclusion that Mattesia YHD is a new species that infects S. invicta. Furthermore, high sequence identity between Mattesia YHD, M. geminata (95.7%), and O. elektroscirrha (86.2%) correctly place the YHD organism in the Mattesia genus and Neogregarinorida order. Oligonucleotide primer pairs were designed to unique areas of the 18S rRNA genes of Mattesia YHD and S. invicta. Multiplex PCR resulted in sensitive and specific detection of Mattesia YHD infection of S. invicta.
Valles
Steven M
SM
USDA-ARS Center for Medical, Agricultural and Veterinary Entomology, 1600 SW 23rd. Drive, Gainesville, FL 32608, USA. svalles@gainesville.usda.ufl.edu
Pereira
Roberto M
RM
D000818:Animals; D016782:Apicomplexa / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal / Q000032:analysis; D006927:Hymenoptera / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000032:analysis*; D017385:Sequence Homology
D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S