Micellar electrokinetic capillary chromatography-electrochemical detection for analysis of biogenic amines in Drosophila melanogaster.
Micellar electrokinetic chromatography coupled to amperometric electrochemical detection was used to investigate the chemical environment of the fruit fly, Drosophila melanogaster. Preliminary studies focused on the employment and optimization of the system to separate electroactive amine-containing molecules present in the head and body of male and female flies. Ultimately, biogenic amines significant to the fly including L-3,4-dihydroxyphenylalanine, dopamine, tyramine, and serotonin were identified and their relative abundance quantified. Transgenic Drosophila with functionally ablated dopamine and serotonin neurons were analyzed to demonstrate the sensitivity of the technique. The separation method developed in this study should offer an advantage in elucidating the critical role that electroactive biogenic amines play in complex physiological processes correlated with Drosophila behavior.
Ream
Paula J
PJ
Department of Chemistry, Pennsylvania State University, 152 Davey Laboratory, University Park, Pennsylvania 16802, USA.
Suljak
Steven W
SW
Ewing
Andrew G
AG
Han
Kyung-An
KA
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000379:methods*; D005260:Female; D006257:Head; D008297:Male
D001679:Biogenic Amines