title: Ovarian primary tissue culture of the kuruma shrimp Marsupenaeus japonicus.
abstract: Cell growth in ovarian primary culture of the kuruma shrimp, Marsupenaeus japonicus, was examined under various culture conditions. The best growth of ovarian cells was obtained in a culture system consisting of double strength of Leibovitz-15 medium supplemented with 10% fetal bovine serum, glucose (1 g/L), proline (0.1 g/L), TC-Yeastolate (1 g/L), and lactalbumin hydrolysate (1 g/L). The cells survived in this medium at 25 degrees C for 45 d. The epithelial-like cells predominated in 10-d-old cultures, covering >80% of the surface area on the bottom of flask. Cells in mitosis were often observed. Cell proliferation was monitored by incorporation of 5-bromo-2'-deoxyuridine (BrdU), an analog of thymidine. 5-Bromo-2'-deoxyuridine-associated cells accounted for 11.5 and 35.0% of cell populations at 2 and 24 h, respectively, after BrdU treatment. Our results provide an improved culture technique for ovarian tissue of the kuruma shrimp.
authors: Kyushu Medical Co., Ltd., Kurume Research Center Building, 2432-3 Aikawa, Kurume, Fukuoka 839-0861, Japan. maeda-bio@ceres.ocn.ne.jp
authors: Minoru
authors: M
authors: Maeda
authors: Eiichi
authors: E
authors: Mizuki
authors: Toshiaki
authors: T
authors: Itami
authors: Michio
authors: M
authors: Ohba
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D005260:Female; D010053:Ovary / Q000166:cytology*; D033561:Penaeidae / Q000166:cytology*; D013997:Time Factors
chemical_list: D003470:Culture Media; D001973:Bromodeoxyuridine