Effects of anabolic steroids and antioxidant vitamins on ethanol-induced tissue injury.
Various mechanisms are involved in the process of ethanol-induced tissue impairment. Oxidative stress and its effects are among the most important. We compared the effects of antioxidant vitamins (vitamin C and E in combination) and steroids (testosterone and nandrolone separately) on the toxicity of ethanol in rats. Animals (male Wistar rats, n = 48) were randomised into following groups-Control, Ethanol, Testosterone, Ethanol + Testosterone, Ethanol + Nandrolone, Ethanol + Vitamins. Alcohol was given daily by gavage in a dose of 5 g/kg of body weight. On the 27th day of the study the animals were sacrificed by decapitation and tissue samples were taken. Metabolic status, parameters of the hepatic metabolism, hormone levels (testosterone, ACTH, corticosterone), lipoperoxidation markers (malondialdehyde and conjugated diens in forebrain cortex and in cerebellum) and advanced glycation end-products were analysed. Tissue samples underwent histological examination. Histological outcomes showed a protective effect of antioxidants on hepatic and cerebellar injury caused by chronic ethanol intake. Anabolic steroids protected especially the central nervous tissue against the toxicity of alcohol. Both, antioxidant vitamins and anabolic steroids protect against the ethanol-induced toxicity, however, this effect is tissue specific.
Celec
Peter
P
Faculty of Medicine, Georg-August University, Göttingen, Germany. petercelec@hotmail.com
Jáni
Peter
P
Smreková
Lucia
L
Mrlian
Andrej
A
Kúdela
Matús
M
Hodosy
Július
J
Boor
Peter
P
Kristová
Viera
V
Jakubovský
Ján
J
Jezová
Daniela
D
Halcák
Lukác
L
Bozek
Peter
P
Slámová
Judita
J
Ulicná
Ol'ga
O
Hojsík
Dalibor
D
Jurkovicová
Ingrid
I
D000324:Adrenocorticotropic Hormone / Q000097:blood; D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002452:Cell Count; D002492:Central Nervous System Depressants / Q000633:toxicity*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control; D003345:Corticosterone / Q000097:blood; D004357:Drug Synergism; D042783:Endothelial Cells / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009277:Nandrolone / Q000494:pharmacology; D009420:Nervous System / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011689:Purkinje Cells / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*; D013739:Testosterone / Q000097:blood / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology*
D045930:Anabolic Agents; D000975:Antioxidants; D002492:Central Nervous System Depressants; D012709:Serum Albumin; D013256:Steroids; D014280:Triglycerides; D014810:Vitamin E; D000431:Ethanol; D013739:Testosterone; D008315:Malondialdehyde; D009277:Nandrolone; D000324:Adrenocorticotropic Hormone; D001205:Ascorbic Acid; D003345:Corticosterone