Induction of tetraploid gynogenesis in the European sea bass (Dicentrarchus labrax L.).
A preliminary study on tetraploid gynogenetic induction in the European sea bass was performed by pressure-blocking the second polar body release and the first cleavage in eggs fertilized with ultraviolet-irradiated sperm. Fertilization of eggs with genetically inactivated sperm produced only haploid development that terminated around hatching. Pressure treatments (8.500 psi for 2 min) applied at 6 and 65 min after fertilization (a.f.) produced variable levels (7-95%) of tetraploid larvae at hatching. A small proportion of mosaics (3.8n/4.2n) was also recorded.
Peruzzi
S
S
IFREMER, Laboratoire de Recherche Piscicole de Mediterranée, Chemin de Maguelone, F-34250 Palavasles-Flots, France. Stefano.Peruzzi@ifremer.fr
Chatain
B
B
D000818:Animals; D001492:Bass / Q000196:embryology / Q000235:genetics*; D002452:Cell Count; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D018869:Heat-Shock Response; D008297:Male; D011123:Polyploidy; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology / Q000528:radiation effects; D014466:Ultraviolet Rays; D015053:Zygote / Q000502:physiology / Q000528:radiation effects