[Study on the diagnostic significance of detecting the expression of AChR-gamma mRNA in rhabdomyosarcoma tissues].
OBJECTIVE
To detect over-expression of AChR-gamma mRNA in rhabdomyosarcoma tissues by duplex RT-PCR and discuss its potential in diagnosis of rhabdomyosarcoma.


METHODS
Duplex RT-PCR was applied to the simultaneous detection of AChR-alpha and gamma subunit messenger RNA in 17 cases of rhabdomyosarcoma (9 ERMS, 6 ARMS, 2 PRMS). 20 cases of non-rhabdomyosarcomous small round cell tumors (6 poorly differentiated synovial sarcomas, 6 ES/PNET, 6 lymphomas, 2 neuroblastomas) and three normal muscle samples were also detected for AChR-alpha and gamma mRNA by the same method.


RESULTS
AChR-alpha and AChR-gamma mRNA were expressed in all the cases of rhabdomyosarcoma. The rate of quantity in both transcripts was AChR-gamma/AChR-alpha >or= 1, but the rate for three normal muscle samples was < 1. Cases of non-rhabdomyosarcomous small round cell tumors were all negative for AChR-gamma.


CONCLUSIONS
AChR-gamma mRNA expression detected by molecular genetic methods is useful in diagnosis and differential diagnosis of rhabdomyosarcoma.
Chang
Bin
B
Department of Pathology, School of Medicine, Shihezi University, Shihezi 832002, China.
Li
Feng
F
Pang
Li-juan
LJ
Hu
Wen-hao
WH
Lu
Tian-cai
TC
Li
Hong-an
HA
Wang
Jian
J
Zhu
Xiong-zeng
XZ
Shi
Da-ren
DR
D003937:Diagnosis, Differential; D006801:Humans; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000235:genetics*; D011978:Receptors, Nicotinic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000175:diagnosis*
C539275:CHRNG protein, human; D021122:Protein Subunits; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic