title: mTOR-dependent regulation of ribosomal gene transcription requires S6K1 and is mediated by phosphorylation of the carboxy-terminal activation domain of the nucleolar transcription factor UBF.
abstract: Mammalian target of rapamycin (mTOR) is a key regulator of cell growth acting via two independent targets, ribosomal protein S6 kinase 1 (S6K1) and 4EBP1. While each is known to regulate translational efficiency, the mechanism by which they control cell growth remains unclear. In addition to increased initiation of translation, the accelerated synthesis and accumulation of ribosomes are fundamental for efficient cell growth and proliferation. Using the mTOR inhibitor rapamycin, we show that mTOR is required for the rapid and sustained serum-induced activation of 45S ribosomal gene transcription (rDNA transcription), a major rate-limiting step in ribosome biogenesis and cellular growth. Expression of a constitutively active, rapamycin-insensitive mutant of S6K1 stimulated rDNA transcription in the absence of serum and rescued rapamycin repression of rDNA transcription. Moreover, overexpression of a dominant-negative S6K1 mutant repressed transcription in exponentially growing NIH 3T3 cells. Rapamycin treatment led to a rapid dephosphorylation of the carboxy-terminal activation domain of the rDNA transcription factor, UBF, which significantly reduced its ability to associate with the basal rDNA transcription factor SL-1. Rapamycin-mediated repression of rDNA transcription was rescued by purified recombinant phosphorylated UBF and endogenous UBF from exponentially growing NIH 3T3 cells but not by hypophosphorylated UBF from cells treated with rapamycin or dephosphorylated recombinant UBF. Thus, mTOR plays a critical role in the regulation of ribosome biogenesis via a mechanism that requires S6K1 activation and phosphorylation of UBF.
authors: Trescowthick Research Laboratories, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, USA.
authors: Katherine M
authors: KM
authors: Hannan
authors: Yves
authors: Y
authors: Brandenburger
authors: Anna
authors: A
authors: Jenkins
authors: Kerith
authors: K
authors: Sharkey
authors: Alice
authors: A
authors: Cavanaugh
authors: Lawrence
authors: L
authors: Rothblum
authors: Tom
authors: T
authors: Moss
authors: Gretchen
authors: G
authors: Poortinga
authors: Grant A
authors: GA
authors: McArthur
authors: Richard B
authors: RB
authors: Pearson
authors: Ross D
authors: RD
authors: Hannan
mesh_terms: D000818:Animals; D002455:Cell Division; D004275:DNA, Ribosomal / Q000235:genetics*; D004789:Enzyme Activation; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D035463:Pol1 Transcription Initiation Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism*; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal; D035463:Pol1 Transcription Initiation Complex Proteins; D011994:Recombinant Proteins; C491074:Rrn3 protein, mouse; C063844:transcription factor UBF; D011494:Protein Kinases; C546843:mTOR protein, mouse; D019893:Ribosomal Protein S6 Kinases; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus