[Cimetidine inhibits the adhesion of cancer cells with sialyl Lewis epitope onto the vascular endothelium].
The attachment of circulating cancer cells to vascular endothelium is considered an important initial step in hematogenous metastasis. We believe that hematogenous metastasis can be inhibited by blocking the adhesion of cancer cells to vascular endothelium. We demonstrated that cimetidine suppressed the expression of E-selectin on the surface of HUVECs, which was a ligand to the sialyl Lewis (sLe) epitope. Thereby, adhesion of HT-29 cells to HUVECs was inhibited by cimetidine pretreatment. In this study, adhesion between cancer cells and HUVECs was observed by a high-speed video recording system. We examined whether or not cimetidine inhibited the adhesion of cancer cells with the sLe epitope, such as gastric, esophageal and breast cancers, to HUVECs. Cimetidine was able to block the adhesion of gastric, esophageal and breast cancer cells with the sLe epitope. We conclude that cimetidine would be effective for inhibiting hematogenous metastasis on gastric, esophageal and breast cancer cells with the expression of sLe epitope.
Hayashi
Atsuhiro
A
Dept. of Surgery, Second Teaching Hospital, Fujita Health University.
Kobayashi
Kenichi
K
Imaeda
Yoshihiro
Y
Matsumoto
Sumio
S
D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000502:physiology; D002927:Cimetidine / Q000494:pharmacology*; D019040:E-Selectin / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D000939:Epitopes; D019073:HT29 Cells; D006801:Humans; D009360:Neoplastic Cells, Circulating; D014471:Umbilical Veins / Q000166:cytology
D000951:Antigens, Neoplasm; C042317:DU-PAN-2 antigen, human; D019040:E-Selectin; D000939:Epitopes; D002927:Cimetidine