title: The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy.
abstract: Circulating IgG antibody reactivity and excreted egg counts were investigated in 489 Kenyans given chemotherapy for schistosomiasis mansoni. Antibody reactivity was measured in ELISA, using either unfractionated aqueous soluble constituents of Schistosoma mansoni eggs (SEA) or CEF6 (a soluble fraction of S. mansoni eggs containing two cationic antigens) as the antigen source. Antibody reactivity for each antigen source was strongly associated with egg counts, both pre- and post-treatment. Approximately 6 months after chemotherapy, egg counts were zero in 84% of the subjects. The mean optical densities (OD) measured in the post-treatment ELISA were 60% (CEF6) or 45% (SEA) lower than the pre-treatment values, the reduction in the OD with CEF6 as antigen source being significantly greater than that observed with SEA (P <0.001). The usefulness of an assay for antibody reactivity in monitoring the effects of the treatment of schistosomiasis is discussed.
authors: School of Biological Sciences, University of Wales Bangor, Deiniol Road, Bangor LL57 2UW, UK. m.doenhoff@bangor.ac.uk
authors: M J
authors: MJ
authors: Doenhoff
authors: J G
authors: JG
authors: Wheeler
authors: K
authors: K
authors: Tricker
authors: J V
authors: JV
authors: Hamilton
authors: R F
authors: RF
authors: Sturrock
authors: A E
authors: AE
authors: Butterworth
authors: J H
authors: JH
authors: Ouma
authors: G G
authors: GG
authors: Mbugua
authors: C
authors: C
authors: Kariuki
authors: D
authors: D
authors: Koech
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010063:Ovum / Q000276:immunology; D010270:Parasite Egg Count; D012555:Schistosomiasis mansoni / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G