title: Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica, Pasteurella multocida and Actinobacillus pleuropneumoniae.
abstract: Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems remained, especially in terms of reduced laying rates. Therefore, experimental vaccines were reformulated with various bacterial antigens of the swine respiratory diseases. After immunizing hens with the antigens, antibody profiles and other effects including laying rates were investigated and compared to those of the previous study. Profiles of antibody titers were very similar with those of the previous study. However, side effects, such as depression, weakness, reduction of laying rates and mortality, were dramatically lowered and laying rates were increased in hens injected with certain experimental vaccines. In particular, laying rates of hens injected with vaccines against atrophic rhinitis were increased up to 84% by injecting a vaccine composed of only the DNTs of B. bronchiseptica and P. multocida D:4. Efficacies of the vaccines against swine pneumonic pasteurellosis and pleuropneumonia were very similar with those of the previous study. These results suggest that new vaccines could be effective in the production of egg yolk antibodies against the causative agents of swine respiratory diseases.
authors: Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, 441-744, Korea.
authors: Na Ri
authors: NR
authors: Shin
authors: In Soo
authors: IS
authors: Choi
authors: Jong Man
authors: JM
authors: Kim
authors: Won
authors: W
authors: Hur
authors: Han Sang
authors: HS
authors: Yoo
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification; D016950:Bordetella bronchiseptica / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004530:Egg Yolk / Q000382:microbiology; D005260:Female; D007136:Immunoglobulins / Q000235:genetics*; D010058:Oviposition; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000276:immunology*; D012703:Serotyping; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C016512:IgY; D007136:Immunoglobulins