title: Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids.
abstract: Combination of capillary electrophoresis with mass spectrometry (CE-MS) allows generation of polypeptide patterns of body fluids. In a single CE-MS (45 min) run more than 600 polypeptides were analyzed in hemodialysis fluids obtained with different membranes (high-flux/low-flux). Larger polypeptides (M(r) > 10 000) were almost exclusively present in high-flux dialysates only, while in low-flux dialysates additional small polypeptides were detected. Comparison to the normal urine pattern yielded a surprisingly low consensus: a number of polypeptides present in urine were missing. We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers.
authors: Mosaiques Diagnostics and Therapeutics AG, Feodor-Lynen-Strasse 5, 30625 Hannover, Germany.
authors: Thorsten
authors: T
authors: Kaiser
authors: Alena
authors: A
authors: Hermann
authors: Jan T
authors: JT
authors: Kielstein
authors: Stefan
authors: S
authors: Wittke
authors: Sebastian
authors: S
authors: Bartel
authors: Ronald
authors: R
authors: Krebs
authors: Frank
authors: F
authors: Hausadel
authors: Meike
authors: M
authors: Hillmann
authors: Igor
authors: I
authors: Golovko
authors: Peer
authors: P
authors: Koester
authors: Hermann
authors: H
authors: Haller
authors: Eva M
authors: EM
authors: Weissinger
authors: Danilo
authors: D
authors: Fliser
authors: Harald
authors: H
authors: Mischak
mesh_terms: D003956:Dialysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010455:Peptides