title: High density of immobilized galactose ligand enhances hepatocyte attachment and function.
abstract: Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6'-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm(2) on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 x 10(5) cells/cm(2), attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface.
authors: Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597.
authors: Chao
authors: C
authors: Yin
authors: Lei
authors: L
authors: Ying
authors: Peng-Chi
authors: PC
authors: Zhang
authors: Ren-Xi
authors: RX
authors: Zhuo
authors: En-Tang
authors: ET
authors: Kang
authors: Kam W
authors: KW
authors: Leong
authors: Hai-Quan
authors: HQ
authors: Mao
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D048429:Cell Size; D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D005690:Galactose / Q000737:chemistry* / Q000378:metabolism; D022781:Hepatocytes / Q000502:physiology* / Q000648:ultrastructure; D008024:Ligands; D015394:Molecular Structure; D037121:Plant Lectins / Q000378:metabolism; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D013499:Surface Properties; D014508:Urea / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D000418:Albumins; D008024:Ligands; D037121:Plant Lectins; D011093:Polyethylene Terephthalates; C006903:carbopol 940; D014508:Urea; D016650:Fluorescein-5-isothiocyanate; D005690:Galactose