Interlaboratory evaluation of hematocytometer method of inoculum preparation for testing antifungal susceptibilities of filamentous fungi.
A three-laboratory study was performed to evaluate conidium counting in a hematocytometer as a technique of inoculum preparation for susceptibility testing of Aspergillus spp. In addition, inocula were quantified by colony counting and optical density determination. The agreement and correlation coefficient between conidium and colony quantifications were 89.2% and 0.73 (P < 0.01). Correlations with optical density determination were not significant.
Rodriguez-Tudela
J L
JL
Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Spain.
Chryssanthou
Erja
E
Petrikkou
Evangelia
E
Mosquera
Juan
J
Denning
David W
DW
Cuenca-Estrella
Manuel
M
D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009172:Mycology / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D000935:Antifungal Agents