title: Carbonic anhydrase inhibitors: inhibition of human and murine mitochondrial isozymes V with anions.
abstract: In addition to sulfonamides, metal complexing anions represent the second class of inhibitors of the zinc enzyme carbonic anhydrase (CA, EC 4.2.1.1). The first inhibition study of the mitochondrial isozyme CA V (of murine and human origin) with anions is reported here. Inhibition data of the cytosolic isozymes CA I and CA II as well as the membrane-bound isozyme CA IV with a large number of anionic species such as halides, pseudohalides, bicarbonate, nitrate, hydrosulfide, arsenate, sulfamate, and sulfamidate and so on, are also provided for comparison. Isozyme V has an inhibition profile by anions completely different to those of CA I and IV, but similar to that of hCA II, which may have interesting physiological consequences. Similarly to hCA II, the mitochondrial isozymes show micro-nanomolar affinity for sulfonamides such as sulfanilamide and acetazolamide.
authors: Università degli Studi di Firenze, Dipartimento di Biologia Animale e Genetica, Via Romana 17-19, 50122 Firenze, Italy.
authors: Marco
authors: M
authors: Franchi
authors: Daniela
authors: D
authors: Vullo
authors: Enzo
authors: E
authors: Gallori
authors: Jochen
authors: J
authors: Antel
authors: Michael
authors: M
authors: Wurl
authors: Andrea
authors: A
authors: Scozzafava
authors: Claudiu T
authors: CT
authors: Supuran
mesh_terms: D000818:Animals; D000838:Anions / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000378:metabolism; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D024404:Carbonic Anhydrase V / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002384:Catalysis; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D000838:Anions; D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D013449:Sulfonamides; D002245:Carbon Dioxide; D024404:Carbonic Anhydrase V