Effect of melatonin on cholestatic oxidative stress under constant light exposure.
This study was designed to evaluate the effect of melatonin on cholestatic oxidative stress under constant light exposure. Cholestasis was induced by double ligature and section of the extra-hepatic bile duct. Melatonin was injected i.p.(1000 microg kg(-1) day(-1)). Malondialdehyde, reduced glutathione, catalase, superoxide dismutase, glutathione reductase, peroxidase and transferase were determined in liver. After bile-duct obstruction and under constant light exposure, an increase in malondialdehyde (p < 0.05) and a slight decrease in reduced glutathione were seen. Enzyme activity, with the exception of glutathione reductase, had significantly diminished. After melatonin administration, malondialdehyde fell (p < 0.001), whereas there was an increase in reduced glutathione (p < 0.0001) compared with untreated controls. Constant light exposure was associated with an increase in hepatic oxidative stress. Treatment with melatonin decreased lipid peroxide synthesis, and permitted a recovery of both reduced glutathione and scavenger enzyme activity.
Cruz
Adolfo
A
Department of General Surgery, Reina Sofía University Hospital, Córdoba, Spain.
Padillo
Francisco J
FJ
Granados
Jose
J
Túnez
Isaac
I
Muñoz
M Carmen
MC
Briceño
Javier
J
Pera-Madrazo
Carlos
C
Montilla
Pedro
P
D000818:Animals; D002779:Cholestasis / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D008027:Light; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
D008315:Malondialdehyde; D005978:Glutathione; D008550:Melatonin