title: Increased transmigration of G-CSF-mobilized peripheral blood CD34+ cells after overnight storage at 37 degrees C.
abstract: BACKGROUND
  G-CSF-mobilized PBPCs are utilized in allogeneic and autologous PBPC transplants. Homing, adhesion, and transmigration of hematopoietic CD34+ cells are required for successful engraftment. Hematopoietic CD34+ cells undergo directional migration toward the CXCR4 receptor ligand stromal-derived factor-1 (SDF-1). Limited data are available on the effects of liquid storage and cryopreservation on PBPC CD34+ cells.
  
  
  METHODS
  Magnetic-assisted cell sorting (MACS)-selected CD34+ cells were assayed for retention of in vitro transmigration and phenotypic changes of unit-matched liquid-stored and cryopreserved PBPC samples from healthy donors. Studies evaluated whether transmigration of CD34+ cells in Iscove's modified Dulbecco's medium plus 1 percent HSA alone or in medium supplemented with SCF or allogeneic plasma was affected by overnight incubation at 37 degrees C, relative to nonincubated conditions.
  
  
  RESULTS
  Transmigration was maintained during liquid storage at 1 to 6 degrees C during a 2-day period and in unit-matched cryopreserved-thawed samples that had been initially liquid stored. Overnight incubation at 37 degrees C of MACS-selected unit-matched liquid-stored or cryopreserved-thawed CD34+ cells resulted in substantially increased transmigration, in particular with noncoated filters chemoattracted with the chemokine SDF-1.
  
  
  CONCLUSIONS
  CD34+ cell transmigration was comparable between liquid-stored and cryopreserved samples, and both demonstrated similar increases after overnight incubation at 37 degrees C.
authors: American Red Cross, Blood & Cell Therapy Development Department, Holland Laboratory, Rockville, Maryland, USA. greco@usa.redcross.org
authors: Nicholas J
authors: NJ
authors: Greco
authors: Wendy R
authors: WR
authors: Lee
authors: Gary
authors: G
authors: Moroff
mesh_terms: D018952:Antigens, CD34 / Q000097:blood*; D001773:Blood Cells / Q000187:drug effects* / Q000276:immunology*; D001793:Blood Preservation; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D013696:Temperature; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016178:Granulocyte-Macrophage Colony-Stimulating Factor