Autologous red cells derived from cord blood: collection, preparation, storage and quality controls with optimal additive storage medium (Sag-mannitol).
To investigate whether packed red cells (PRCs) prepared from autologous cord blood-packed red cells (AC-PRCs) could be used as an alternative for homologous-packed red cells (H-PRCs), we developed a system to collect and prepare AC-PRCs and determined standard storage parameters during 35 days of storage in extended storage medium (Sag-mannitol). We collected and fractionated cord blood from 390 newborns. The amount and quality of the AC-PRCs were analysed. The bacterial contamination rate was 1.84%. Twelve AC-PRCs were stored for 35 days, and standard laboratory parameters were measured at day 1 and day 35. The initial laboratory parameters of the AC-PRCs were similar to the parameters of the H-PRCs. After 35 days, the AC-PRCs displayed an increased haemolysis rate compared to H-PRCs (1.1 versus 0.2%) and also a significant decreased adenosine triphosphate value (1.2 versus 2.3 micromol L(-1)). Haemoglobin, haematocrit and pH were comparable in both groups. AC-PRCs meet the quality criteria for H-PRCs after 35 days. Utilizing a closed collection system for cord blood and an extended storage medium will increase safety and quality and facilitate the routine transfusion of autologous red cells derived from cord blood.
Garritsen
H S P
HS
Department of Transfusion Medicine, Städtisches Klinikum, Braunschweig, Germany. h.garritsen@klinikum-braunschweig.de
Brune
T
T
Louwen
F
F
Wüllenweber
J
J
Ahlke
C
C
Cassens
U
U
Witteler
R
R
Sibrowski
W
W
D000255:Adenosine Triphosphate / Q000097:blood; D001419:Bacteria; D001793:Blood Preservation / Q000379:methods / Q000592:standards; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D001804:Blood Transfusion, Autologous / Q000379:methods* / Q000592:standards; D017707:Erythrocyte Transfusion / Q000379:methods* / Q000592:standards; D005312:Fetal Blood; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008353:Mannitol; D011786:Quality Control
D006454:Hemoglobins; D008353:Mannitol; D000255:Adenosine Triphosphate