title: Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis.
abstract: OBJECTIVE
  To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration.
  
  
  METHODS
  Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  
  
  METHODS
  To develop DLK in rabbit eyes, 25 microL of lipopolysaccharide (LPS) solution at a concentration of 50 microg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed.
  
  
  RESULTS
  Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils.
  
  
  CONCLUSIONS
  The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.
authors: Minamiaoyama Eye Clinic, Tokyo, Japan. naokato@bc.iij4u.or.jp
authors: Naoko
authors: N
authors: Asano-Kato
authors: Ikuko
authors: I
authors: Toda
authors: Shigeto
authors: S
authors: Shimmura
authors: Tomoko
authors: T
authors: Noda-Tsuruya
authors: Kazumi
authors: K
authors: Fukagawa
authors: Masaru
authors: M
authors: Yoshinaga
authors: Akihiro
authors: A
authors: Matsukawa
authors: Kazuo
authors: K
authors: Tsubota
mesh_terms: D000818:Animals; D002465:Cell Movement; D003319:Corneal Stroma / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D016209:Interleukin-8 / Q000378:metabolism*; D007634:Keratitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D020731:Keratomileusis, Laser In Situ / Q000009:adverse effects*; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000473:pathology*; D011817:Rabbits; D012486:Salmonella typhimurium
chemical_list: D016209:Interleukin-8; D008070:Lipopolysaccharides