title: [Preparation and identification of egg yolk antibodies against HLA-A* 0201 heavy chain].
abstract: OBJECTIVE
  To prepare a high-titer specific and efficient egg yolk immunoglobulin (IgY).
  
  
  METHODS
  The antigen of HLA-A* 0201 heavy chain was used to immunize the hens together with Freud's adjuvant and the eggs these hens laid were collected. IgY was purified from the egg yolk by water extraction, salt precipitation with ammonium sulfate and dialysis. The immunoactivity of the IgY was measured by enzyme-linked immunosorbent assay (ELISA) and its purity was determined by SDS-PAGE.
  
  
  RESULTS
  The titer of IgY was 1  10(6) as shown by ELISA, with protein concentration of 9.77 mg/ml. The purity of the resultant IgY was more than 90% as suggested by SDS-PAGE analysis.
  
  
  CONCLUSIONS
  The antigen of HLA-A* 0201 heavy chain along with complete Freund adjuvant is effective to elicit an immune response of hens for producing IgY antibodies in high yields.
authors: Institute of Molecular Immunology, First Military Medical University, Guangzhou 510515, China. mengxj163@163.net
authors: Xiao-jing
authors: XJ
authors: Meng
authors: Min-jie
authors: MJ
authors: Meng
authors: Xing-mei
authors: XM
authors: Linlai
authors: Ming-qian
authors: MQ
authors: Zhou
authors: Xiao-ning
authors: XN
authors: Wang
mesh_terms: D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D015234:HLA-A Antigens; C016512:IgY; D007136:Immunoglobulins