Induced recovery of defective membrane expression of a CC chemokine receptor 5 mutant by phytohemagglutinin.
CC chemokine receptor 5 (CCR5) is a member of the G-protein-coupled receptor superfamily. It plays an important role in macrophage tropic human immunodeficiency virus-1 entry and in some inflammatory reactions. CCR5-893(-) is a single-nucleotide deletion that results in complete truncation of the C tail of the gene product. We detected CCR5-893(-) in a sample of patients infected with non-tuberculosis mycobacteria and found that it was maintained heterozygously with a frequency of 2%. There is no association between this mutation and any immunodeficiency. Membrane expression of CCR5-893(-) was substantially reduced compared to the wild type, but this defective surface presentation recovered greatly recovered in the presence of 2 mg l(-1) phytohemagglutinin (PHA). However, PHA inducement did not affect the total intracellular expression of CCR5-893(-) or wild-type CCR5. Thus we suggest there exist some PHA-induced factor(s) that could mediate the presentation of truncated CCR5.
Qi
Q-Y
QY
Laboratory of Population & Quantitative Genetics, The State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
Wang
F
F
Zhang
H-T
HT
Wang
J-C
JC
Xiao
H-P
HP
Wang
M-H
MH
Han
Y-F
YF
Zhang
R-M
RM
Tao
S-H
SH
Luo
Z W
ZW
D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry; D006801:Humans; D009154:Mutation; D009164:Mycobacterium Infections / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D019713:Receptors, CCR5 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D010835:Phytohemagglutinins; D019713:Receptors, CCR5