title: Insertion of an En/Spm-related transposable element into a floral homeotic gene DUPLICATED causes a double flower phenotype in the Japanese morning glory.
abstract: Mutations in a floral homeotic gene DUPLICATED (DP) in the Japanese morning glory (Ipomoea nil) cause a substitution of reproductive organs to perianth organs (petals and sepals). This phenotype is similar to loss-of-function phenotypes of the C-function MCM1, AGAMOUS, DEFICIENS and SRF (MADS)-box gene family of transcription factors. DP was isolated using the consensus sequence of C-function MADS-box genes. Its intron-exon structure was well conserved beyond species, and it belongs to the FARINELLI (FAR)-pMADS3 subclass of C-function MADS-box genes. In a dp mutant, an Enhancer/Suppressor-mutator (En/Spm)-related transposable element, transposable element of Pharbitis nil (Tpn)-botan, was inserted in the second intron of DP gene, and the subsequent excision event led to a deletion of a substantial part of the original Tpn element and DP genome. A segment of Tpn1-related transposable element was identified at the recent insertion site of the wild-type DP genome. This transposable element sequence was present in all Ipomoea species tested. This finding suggests that the insertion event originated in an ancestral species of genus Ipomoea.
authors: Department of Biological Science, Graduate School of Science, Kyushu University, Fukuoka 812-8581, Japan. enitascb@mbox.nc.kyushu-u.ac.jp
authors: Eiji
authors: E
authors: Nitasaka
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D005838:Genotype; D027741:Ipomoea / Q000235:genetics*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; D026161:MADS Domain Proteins; D010940:Plant Proteins