[Effects of Fall-39 mRNA expression and antibacterial activity of human pulmonary gland epithelial cells induced by BCG].
OBJECTIVE
To disclose whether BCG can enhance Fall-39 gene expression in human pulmonary gland epithelial cells, and to isolate and identify the bioactive proteins that stimulate Fall-39 gene expression in human pulmonary gland epithelial cells.


METHODS
Fall-39 mRNA expression in SPC-A-1 cells was detected by using RT-PCR. The cell wall proteins were isolated by sucrose density-gradient centrifugation and fractionated by Sephadex G-75 column chromatography. The antibacterial activity of the cell culture supernatant was determined by agarose radial diffusion assay.


RESULTS
The enhanced expression of Fall-39 mRNA was dose- and time-dependent. BCG cell wall proteins fraction 4 had an activity that remarkably enhanced Fall-39 mRNA expression in SPC-A-1 cells and augmented antibacterial activity of the cell culture supernatant.


CONCLUSIONS
These results indicated that BCG could stimulate Fall-39 mRNA expression in human pulmonary gland epithelial cells, thus providing an evidence base for shaping well a new strategy to enhance mucosa antibiotic peptide expression for the prevention and treatment of mucosal infections.
Zhu
Ling
L
Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Feng
Yun
Y
Huang
Ning
N
Wang
Boyao
B
Chen
Jiayu
J
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001500:BCG Vaccine / Q000494:pharmacology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology*; D009163:Mycobacterium bovis / Q000737:chemistry*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001500:BCG Vaccine; C091425:FALL 39; D010455:Peptides; D012333:RNA, Messenger