Ex vivo perfusion of canine pancreaticoduodenal allografts using class-II-specific monoclonal antibody delays the onset of acute rejection.
In the following study, we investigated whether ex vivo perfusion of canine pancreaticoduodenal allografts prior to transplantation using a class-II-specific monoclonal antibody (MoAb) OKIa1) could prevent acute rejection. Untreated grafts were rejected within 6 days after transplantation, and all of these recipients suffered severe hyperglycemia. In contrast, in recipients who received grafts which underwent ex vivo class-II-specific MoAb perfusion treatment, the mean urinary amylase levels were sustained significantly higher (11,733 +/- 4493 vs. 3274 +/- 2108 U/L on day 7, P < 0.005), and mean fasting blood glucose (FBG) levels remained within the normal range (13.4 +/- 5.8 vs. 23.4 +/- 3.9 mM on day 7, P < 0.0005). Low doses of cyclosporin A (CsA) were necessary in order to maintain lower FBG levels. Histopathology analysis on day 7 after transplantation showed that endotheliitis and necrosis were much less prominent in the MoAb-treated grafts. In the light of our results, we conclude that ex vivo perfusion of canine pancreaticoduodenal allografts using a class-II-specific MoAb is effective in delaying the onset of acute rejection, and low doses of CsA could extend this effect.
Miyoshi
K
K
First Department of Surgery, Okayama University Medical School, Okayama, Japan.
Sakagami
K
K
Orita
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D003712:Dendrites / Q000276:immunology / Q000473:pathology; D004285:Dogs; D004386:Duodenum / Q000637:transplantation*; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008297:Male; D023421:Models, Animal; D016035:Pancreas Transplantation / Q000276:immunology* / Q000379:methods; D010180:Pancreatectomy; D010477:Perfusion; D020858:Tissue and Organ Harvesting / Q000379:methods; D014184:Transplantation, Homologous / Q000276:immunology / Q000379:methods
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II