The B-cell transcription factors BSAP, Oct-2, and BOB.1 and the pan-B-cell markers CD20, CD22, and CD79a are useful in the differential diagnosis of classic Hodgkin lymphoma.
Even with routine immunohistochemical evaluation, distinguishing classic Hodgkin lymphoma (CHL) from diffuse large B-cell lymphoma (DLBCL) or anaplastic large cell lymphoma (ALCL) can be difficult. In these cases, the transcription factors (B cell-specific activator protein [BSAP], octamer-binding transcription factor 2 [Oct-2], and B-cell Oct-binding protein 1 [BOB.1]) and the pan-B-cell markers (CD20, CD22, and CD79a) may aid in clarifying the diagnosis. In 57 cases of CHL, 5 cases of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), and 33 cases of non-Hodgkin lymphoma (25 DLBCL and 8 ALCL) we found the transcription factor phenotype BSAP+ and either Oct-2- or BOB.1- to be predictive of CHL; BSAP+/Oct-2+/BOB.1+ was predictive of NLPHL or DLBCL, while BSAP- was predictive of ALCL. Expression of all 3 pan-B-cell markers was seen only in NLPHL and DLBCL; positivity for a single B-cell marker was present only in CHL. Thus, together, the transcription factors and pan-B-cell markers might be useful in the differential diagnosis of CHL.
Browne
Patrick
P
Dept of Pathology, IMPATH, 5300 McConnell Ave, Los Angeles, CA 90066, USA.
Petrosyan
Karen
K
Hernandez
Antonio
A
Chan
Joel A
JA
D015703:Antigens, CD / Q000032:analysis*; D018951:Antigens, CD20 / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D051925:CD79 Antigens; D015815:Cell Adhesion Molecules; D004268:DNA-Binding Proteins / Q000032:analysis*; D003937:Diagnosis, Differential; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis*; D008221:Lymphoid Tissue / Q000737:chemistry; D016393:Lymphoma, B-Cell / Q000175:diagnosis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis; D050813:Octamer Transcription Factor-2; D051757:PAX5 Transcription Factor; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D051918:Sialic Acid Binding Ig-like Lectin 2; D015534:Trans-Activators / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*
D015703:Antigens, CD; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D051925:CD79 Antigens; C497881:CD79A protein, human; D015815:Cell Adhesion Molecules; D004268:DNA-Binding Proteins; D037102:Lectins; D050813:Octamer Transcription Factor-2; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C091546:POU2AF1 protein, human; C058435:POU2F2 protein, human; D011947:Receptors, Antigen, B-Cell; D051918:Sialic Acid Binding Ig-like Lectin 2; D015534:Trans-Activators; D014157:Transcription Factors