Analysis of microfouling products formed on metallic surfaces exposed in a marine environment.
Mild steel (MS), stainless steel (SS) and copper (Cu) test panels were immersed in the surface water of Dona Paula Bay over a period of 15 d. During the immersion period data on the hydrography, nutrients and suspended matter were also collected. The suspended matter and fouling products on the MS, SS and Cu panels were analysed for organic carbon (OC), organic nitrogen (ON), chlorophyll a (chl a), protein and carbohydrate concentration and composition, and the dry weight (DW) was recorded. Compared to suspended matter, the chemical and biochemical components of the fouling products showed strong temporal and substratum related differences. The microfouling biomass (as DW, OC, ON, chl a and protein) on all the test panels generally increased over the period of immersion. Carbohydrates were more abundant in the suspended matter whereas fouling products were enriched in proteins. The contribution of protein-carbon to the total carbon increased over the period of immersion for the microfouling products on MS and SS whilst it did not show a consistent trend on Cu. Whereas, the carbohydrate-carbon contribution to the total carbon increased for the fouling products on MS, it did not exhibit a particular pattern on SS or Cu over the period of immersion. Capillary gas chromatographic analysis showed the presence of glucose, galactose, mannose, arabinose, xylose fucose and ribose in both the fouling products and suspended matter. However, there were differences in the relative distribution of these monosaccharides in the suspended matter and the fouling products. Glucose was the most abundant monosaccharide, which showed strong temporal variations in suspended matter. In contrast, the wt % concentrations of individual monosaccharides showed large temporal differences for the fouling products, which were strongly influenced by the period of immersion and the type of test substratum. Glucose and fucose were relatively more abundant in the fouling products on SS and Cu, whilst glucose was the most abundant monosaccharide on MS. The monosaccharide and chemical composition data suggest strong temporal changes in the composition of the fouling products.
D'Souza
Fraddry
F
National Institute of Oceanography, Marine Corrosion and Material Research Division, Dona Paula, 403004 Goa, India.
Bhosle
Narayan B
NB
D001422:Bacterial Adhesion; D002241:Carbohydrates / Q000032:analysis*; D002849:Chromatography, Gas; D003300:Copper / Q000737:chemistry*; D007194:India; D009930:Organic Chemicals / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012623:Seawater / Q000032:analysis*; D013232:Steel / Q000737:chemistry*; D013997:Time Factors
D002241:Carbohydrates; D009930:Organic Chemicals; D011506:Proteins; D013232:Steel; D003300:Copper