title: Anaemia stimulates aquaporin 1 expression in the fetal sheep heart.
abstract: Interstitial fluid fluxes are much greater in the fetus than in the adult, and filtration rates are increased over control in most tissues of the anaemic fetus. Increased capillary filtration may lead to cardiac oedema which, in turn, severely impacts cardiac function. Mechanisms that underlie these differences in flux are incompletely understood. One possible mechanism is an increase in capillary water permeability. Therefore, the goal of our study was to determine the level of expression of the water channel aquaporin 1 (AQP1) during cardiac development and in the anaemic fetal sheep heart. Hearts from chronically instrumented anaemic sheep fetuses and hearts from normal early fetal, late fetal, neonatal and adult sheep were used for Northern and Western analyses and immunohistochemistry. We found that AQP1 mRNA levels were lower in the young fetal left ventricle than in the adult left ventricle (P < 0.05). We also found that cardiac AQP1 expression was increased in anaemic fetuses compared to age-matched controls (P < 0.05). Expression of AQP1 in all groups was greatest in the microvascular endothelium. These data suggest that AQP1 plays an important role in the physiological accommodation to fetal anaemia.
authors: Departments of Physiology and Pharmacology, Obstetrics, Medicine (Cardiology), and Heart Research Center, Oregon Health and Science University, Portland, OR 97239, USA.
authors: S
authors: S
authors: Jonker
authors: L E
authors: LE
authors: Davis
authors: J D W
authors: JD
authors: van der Bilt
authors: B
authors: B
authors: Hadder
authors: A R
authors: AR
authors: Hohimer
authors: G D
authors: GD
authors: Giraud
authors: K L
authors: KL
authors: Thornburg
mesh_terms: D000222:Adaptation, Physiological; D000375:Aging / Q000378:metabolism*; D000740:Anemia / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D051398:Aquaporin 1; D020346:Aquaporins / Q000378:metabolism*; D005260:Female; D006352:Heart Ventricles / Q000196:embryology* / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012756:Sheep
chemical_list: D020346:Aquaporins; D051398:Aquaporin 1