Leptin increase is associated with markers of the hemostatic system in obese healthy women.
BACKGROUND
Leptin, a hormone secreted by the adipose tissue, might be a link between obesity and increased morbidity for cardiovascular disease. Leptin exerts proinflammatory, pro-angiogenic actions by activating a specific receptor (Ob-Rb) which is expressed in human endothelial cells. Thus, a link may exist between leptin expression and endothelial dysfunction.


OBJECTIVE
We sought to determine whether in obese women there is a correlation between leptin levels, endothelial perturbation and coagulative activation.


METHODS
Circulating levels of leptin, von Willebrand Factor (VWF), factor (F)VIIa, prothrombin fragment 1 + 2 (F1+2), were measured in 51 non-diabetic, obese women and in 51 normal-weight subjects, using immunoenzymatic assays.


RESULTS
Obese women had significantly higher levels of leptin, VWF, FVIIa, F1+2 compared with healthy women. Simple correlation coefficients showed significant correlation between leptin and either VWF, FVIIa, or F1+2 concentrations. A multiple linear regression analysis, performed to quantify further the relationship between leptin levels and the above-mentioned variables as well as the inflammatory marker C-reactive protein (CRP) and including age, body mass index (BMI), waist-hip ratio (WHR) and lipid parameters as potential confounders, revealed that only FVIIa and VWF were independently related to leptin levels. Reduction in adipose tissue after weight loss resulted in a decrease in both circulating leptin and endothelial and coagulative activation markers.


CONCLUSIONS
We suggest that leptin might have pro-atherogenic effects in vivo, with a mechanism involving endothelial cell activation.
Guagnano
M T
MT
Center of Excellence on Aging and Department of Medicine, University of Chieti G D'Annunzio, School of Medicine, Chieti, Italy.
Romano
M
M
Falco
A
A
Nutini
M
M
Marinopiccoli
M
M
Manigrasso
M R
MR
Basili
S
S
Davì
G
G
D000328:Adult; D000886:Anthropometry; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation; D016022:Case-Control Studies; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006487:Hemostasis; D006801:Humans; D007249:Inflammation / Q000097:blood; D020738:Leptin / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D019851:Thrombophilia / Q000097:blood; D015431:Weight Loss
D015415:Biomarkers; D020738:Leptin