Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization.
Metabolomic characteristics in boar spermatozoa were studied using [1,2-(13)C(2)]glucose and mass isotopomer analysis. In boar spermatozoa, glycolysis was the main pathway of glucose utilization producing lactate/pyruvate, whereas no gluconeogenesis was seen. Slight glycogen synthesis through the direct pathway and some incorporation of pyruvate into the Krebs cycle also took place. Neither RNA ribose-5-phosphate nor fatty acid synthesis from glucose occurred despite the detection of pyruvate dehydrogenase activity. In contrast to the known metabolic activities in dog sperm, boar spermatozoa have low levels of energy production and biosynthetic activities suggesting two different metabolic profiles for the two different phenotypes.
Marin
Silvia
S
Department of Biochemistry and Molecular Biology, University of Barcelona, C/ Martí i Franqués 1, Barcelona 08028, Spain.
Chiang
Kelly
K
Bassilian
Sara
S
Lee
Wai-Nang Paul
WN
Boros
Laszlo G
LG
Fernández-Novell
Josep Maria
JM
Centelles
Josep Joan
JJ
Medrano
Antonio
A
Rodriguez-Gil
Joan Enric
JE
Cascante
Marta
M
D000818:Animals; D002247:Carbon Isotopes; D002952:Citric Acid Cycle / Q000502:physiology; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis; D007773:Lactates / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010427:Pentose Phosphate Pathway / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
D002247:Carbon Isotopes; D005227:Fatty Acids; D007773:Lactates; D006003:Glycogen; D005947:Glucose