title: Ascorbic acid supplementation does not improve efficacy of meso-dimercaptosuccinic acid treatment in lead-exposed suckling rats.
abstract: It was suggested that ascorbic acid as a natural chelating agent can influence lead toxicokinetics and improve chelating properties of dimercaptosuccinic acid (DMSA) in adult rats. In this paper potential benefits of ascorbic acid supplementation, alone or combined with DMSA, in decreasing lead retention in suckling rats were evaluated. Such data in young mammals are not available. L-Ascorbic acid (daily dose 650 mg/kg b.wt.) and/or DMSA (daily dose 91 mg/kg b.wt.) were administered orally to suckling Wistar rats either during ongoing 8-day oral lead exposure (as acetate; daily dose 2 mg lead/kg b.wt.) or after 3-day lead exposure (total dose 12 mg lead/kg b.wt.). Lead concentrations were analysed in the carcass (skeleton), liver, kidneys and brain by atomic absorption spectrometry. By ascorbic acid supplementation lead retention was not reduced under either lead exposure condition. Lead concentration was even increased in the carcass. Treatment with DMSA under both exposure conditions significantly reduced lead in all analysed tissues. Combined treatment with ascorbic acid and DMSA during ongoing lead exposure was substantially less effective than DMSA treatment alone, and did not affect DMSA efficacy when administered after lead exposure. It was concluded that ascorbic acid administered either during or after lead exposure in suckling rats has no beneficial effect on either lead retention or DMSA chelation effectiveness.
authors: Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia. vvarnai@imi.hr
authors: Veda Marija
authors: VM
authors: Varnai
authors: Martina
authors: M
authors: Piasek
authors: Maja
authors: M
authors: Blanusa
authors: Dijana
authors: D
authors: Juresa
authors: Marija
authors: M
authors: Sarić
authors: Krista
authors: K
authors: Kostial
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D000931:Antidotes / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D007854:Lead / Q000378:metabolism* / Q000633:toxicity; D007855:Lead Poisoning / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D004113:Succimer / Q000494:pharmacology*
chemical_list: D000931:Antidotes; D002614:Chelating Agents; D007854:Lead; D004113:Succimer; D001205:Ascorbic Acid