[Altered of zinc finger proteins expression in FL cells following benzo [a] pyrene treatment].
To understand benzo[a]pyrene (B[a]P) mediated cellular responses, and to provide clues to explore molecular mechanism of mutagenesis and carcinogenesis induced by B[a]P.



Two-dimensional electrophoresis (2-DE) was used to investigate the protein expression levels of FL cells after B[a]P exposure, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify the differentially expressed proteins.



Statistical analysis showed that the volumes of 47 protein spots were altered after B[a]P treatment (P<0.05) and 23 proteins were successfully identified, including zinc finger proteins, SWI/SNF related protein, Bromo domain containing domain and other proteins.



These affected proteins may be involved in the cellular responses to B[a]P exposure, and may mediate the B[a]P induced mutagenesis and carcinogenesis.
Gao
Zhi-hua
ZH
Department of Pathophysiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Yang
Jing-hua
JH
Yang
Jun
J
Yu
Ying-nian
YN
D000650:Amnion / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002478:Cells, Cultured; D004260:DNA Repair; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D040901:Proteomics; D016335:Zinc Fingers
D001564:Benzo(a)pyrene