IFN-gamma polymorphisms (IFN-gamma +2109 and IFN-gamma +3810) are associated with severe hepatic fibrosis in human hepatic schistosomiasis (Schistosoma mansoni).
Schistosome infection is a major public health concern affecting millions of people living in tropical regions of Africa, Asia, and South America. Schistosomes cause mild clinical symptoms in most subjects, whereas a small proportion of individuals presents severe clinical disease (as periportal fibrosis (PPF)) that may lead to death. Severe PPF results from an abnormal deposition of extracellular matrix proteins in the periportal spaces due to a chronic inflammation triggered by eggs and schistosome Ags. Extracellular matrix protein production is regulated by a number of cytokines, including IFN-gamma. We have now screened putative polymorphic sites within this gene in a population living in an endemic area for Schistosoma mansoni. Two polymorphisms located in the third intron of the IFN-gamma gene are associated with PPF. The IFN-gamma +2109 A/G polymorphism is associated with a higher risk for developing PPF, whereas the IFN-gamma +3810 G/A polymorphism is associated with less PPF. The polymorphisms result in changes in nuclear protein interactions with the intronic regions of the gene, suggesting that they may modify IFN-gamma mRNA expression. These results are consistent with the results of previous studies. Indeed, PPF is controlled by a major locus located on chromosome 6q22-q23, closely linked to the gene encoding the alpha-chain of the IFN-gamma receptor, and low IFN-gamma producers have been shown to have an increased risk of severe PPF. Together, these observations support the view that IFN-gamma expression and subsequent signal transduction play a critical role in the control of PPF in human hepatic schistosome infection (S. mansoni).
Chevillard
Christophe
C
Immunologie et Génétique des Maladies Parasitaires, Laboratoire de Parasitologie-Mycologie, Faculté de Médecine, Institut National de la Santé et de la Recherche Médicale Unité 399, Marseille, France. christophe.chevillard@medecine.univ-mrs.fr
Moukoko
Carole Eboumbou
CE
Elwali
Nasr-Eldin M A
NE
Bream
Jay H
JH
Kouriba
Bourema
B
Argiro
Laurent
L
Rahoud
Siddig
S
Mergani
Adil
A
Henri
Sandrine
S
Gaudart
Jean
J
Mohamed-Ali
Qurashi
Q
Young
Howard A
HA
Dessein
Alain J
AJ
D000225:Adenine; D004247:DNA / Q000235:genetics / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006147:Guanine; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008103:Liver Cirrhosis / Q000000981:diagnostic imaging / Q000235:genetics* / Q000276:immunology*; D008109:Liver Diseases, Parasitic / Q000000981:diagnostic imaging / Q000235:genetics* / Q000276:immunology*; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000000981:diagnostic imaging / Q000235:genetics* / Q000276:immunology*; D012720:Severity of Illness Index; D014463:Ultrasonography
D009687:Nuclear Proteins; D006147:Guanine; D007371:Interferon-gamma; D004247:DNA; D000225:Adenine