title: Lipoprotein receptors and a disabled family cytoplasmic adaptor protein regulate EGL-17/FGF export in C. elegans.
abstract: Growth factors and morphogens need to be secreted to act on distant cells during development and in response to injury. Here, we report evidence that efficient export of a fibroblast growth factor (FGF), EGL-17, from the Caenorhabditis elegans developing vulva requires the lipoprotein receptor-related proteins Ce-LRP-1 and Ce-LRP-2 and a cytoplasmic adaptor protein, Ce-DAB-1 (Disabled). Lipoprotein receptors are transmembrane proteins best known for their roles in endocytosis. Ce-LRP-1 and Ce-LRP-2 possess a conserved intraluminal domain that can bind to EGL-17, as well as a cytosolic FXNPXY motif that can bind to Ce-DAB-1. Ce-DAB-1 contains signals that confer subcellular localization to Golgi-proximal vesicles. These results suggest a model in which Ce-DAB-1 coordinates selection of receptors and cargo, including EGL-17, for transport through the secretory pathway.
authors: Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors: Darren M
authors: DM
authors: Kamikura
authors: Jonathan A
authors: JA
authors: Cooper
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D020816:Amino Acid Motifs; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D003593:Cytoplasm; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing; D006056:Golgi Apparatus; D049452:Green Fluorescent Proteins; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism; D009154:Mutation; D010750:Phosphoproteins / Q000378:metabolism; D011233:Precipitin Tests; D018110:Receptors, Lipoprotein / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013347:Subcellular Fractions; D020798:Two-Hybrid System Techniques; D014844:Vulva / Q000166:cytology / Q000254:growth & development*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; D029742:Caenorhabditis elegans Proteins; C478773:Dab2 protein, mouse; C105157:Egl-17 protein, C elegans; D036341:Intercellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D010750:Phosphoproteins; D018110:Receptors, Lipoprotein; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors