Use of a gas-sensor array for detecting volatile organic compounds (VOC) in chemically induced cells.
An application of gas sensors for rapid bioanalysis is presented. An array of temperature-modulated semiconductor sensors was used to characterize the headspace above a cell culture. Recombinant Saccharomyces cerevisiae yeast cells, able to respond to 17 beta-estradiol by producing a reporter protein, were used as a model system. Yeast cells had the DNA sequence of the human estrogen receptor stably integrated into the genome, and contained expression plasmids carrying estrogen-responsive sequences and the reporter gene lac-Z, encoding the enzyme beta-galactosidase. The sensor-response profiles showed small but noticeable discrimination between cell samples induced with 17 beta-estradiol and non-induced cell samples. The sensor array was capable of detecting changes in the volatile organic compound composition of the headspace above the cultured cells, which can be associated with metabolic changes induced by a chemical compound. This finding suggests the possibility of using cross-selective gas-sensor arrays for analysis of drugs or bioactive molecules through their interaction with cell systems, with the advantage of providing information on their bioavailability.
Pasini
Patrizia
P
Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. patrizia.pasini@unibo.it
Powar
Nilesh
N
Gutierrez-Osuna
Ricardo
R
Daunert
Sylvia
S
Roda
Aldo
A
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004958:Estradiol / Q000032:analysis / Q000494:pharmacology; D005740:Gases / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements; D009930:Organic Chemicals / Q000032:analysis* / Q000494:pharmacology*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012666:Semiconductors; D013696:Temperature; D014835:Volatilization; D001616:beta-Galactosidase / Q000378:metabolism
D005740:Gases; D009930:Organic Chemicals; D004958:Estradiol; D001616:beta-Galactosidase