title: The transcription factor Eyes absent is a protein tyrosine phosphatase.
abstract: Post-translational modifications provide sensitive and flexible mechanisms to dynamically modulate protein function in response to specific signalling inputs. In the case of transcription factors, changes in phosphorylation state can influence protein stability, conformation, subcellular localization, cofactor interactions, transactivation potential and transcriptional output. Here we show that the evolutionarily conserved transcription factor Eyes absent (Eya) belongs to the phosphatase subgroup of the haloacid dehalogenase (HAD) superfamily, and propose a function for it as a non-thiol-based protein tyrosine phosphatase. Experiments performed in cultured Drosophila cells and in vitro indicate that Eyes absent has intrinsic protein tyrosine phosphatase activity and can autocatalytically dephosphorylate itself. Confirming the biological significance of this function, mutations that disrupt the phosphatase active site severely compromise the ability of Eyes absent to promote eye specification and development in Drosophila. Given the functional importance of phosphorylation-dependent modulation of transcription factor activity, this evidence for a nuclear transcriptional coactivator with intrinsic phosphatase activity suggests an unanticipated method of fine-tuning transcriptional regulation.
authors: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
authors: Tina L
authors: TL
authors: Tootle
authors: Serena J
authors: SJ
authors: Silver
authors: Erin L
authors: EL
authors: Davies
authors: Victoria
authors: V
authors: Newman
authors: Robert R
authors: RR
authors: Latek
authors: Ishara A
authors: IA
authors: Mills
authors: Jeremy D
authors: JD
authors: Selengut
authors: Beth E W
authors: BE
authors: Parlikar
authors: Ilaria
authors: I
authors: Rebay
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D045163:Antibodies, Phospho-Specific / Q000276:immunology; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology* / Q000235:genetics; D004627:Embryonic Induction; D005123:Eye / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D007700:Kinetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D011487:Protein Conformation; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D045163:Antibodies, Phospho-Specific; D029721:Drosophila Proteins; D005136:Eye Proteins; D014157:Transcription Factors; C092694:eya protein, Drosophila; D017027:Protein Tyrosine Phosphatases