Analysis of fentanyl and norfentanyl in human plasma by liquid chromatography-tandem mass spectrometry using electrospray ionization.
This report describes a sensitive and specific high-performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry (MS-MS) method for the detection of subnanogram concentrations of fentanyl and its metabolite norfentanyl in human plasma. The assay was based on a liquid-liquid extraction of 0.5 mL of human plasma, with a lower limit of quantitation (LLOQ) of 0.05 ng/mL. Sample extracts were analyzed using a ThermoQuest TSQ MS-MS interfaced with a Hewlett-Packard series 1100 HPLC and a Phenomenex (30 x 2.00-mm, 5 microLuna C18(2)) column. The intra-assay precision and accuracy ranged from 2.1 to 12.5% for both analytes at concentrations of 0.1, 0.5, 1.0, and 10 ng/mL. The interassay accuracy and precision ranged from 7.34 to 10.95%.
Day
J
J
Center for Human Toxicology, University of Utah, Salt Lake City, Utah 84112, USA.
Slawson
M
M
Lugo
R A
RA
Wilkins
D
D
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D007223:Infant; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization
C080127:norfentanyl; D005283:Fentanyl