A nested alpha-amylase gene in Drosophila ananassae.
The amylase gene family of Drosophila ananassae consists in seven copies, scattered on several chromosomal arms. We have evidenced that a member of the family, Amy35, lies within an intron of a gene homologous to the CG14696 gene of D. melanogaster. This nested arrangement seems restricted to the D. ananassae subgroup. The nested and the nest genes are encoded on opposite strands. Both are actively transcribed in the midgut at the same time, raising the possibility of interference between their mRNAs. Our data also help to elucidate the history of the Amy family, suggesting that Amy35 arose by duplication and translocation from another ancestral locus, into a formerly short intron, in an ancestor of the subgroup.
Da Lage
Jean-Luc
JL
Centre National de la Recherche Scientifique (CNRS), Populations, Génétique et Evolution (UPR 9034), 91198 Gif sur Yvette, France. jldl@pge.cnrs-gif.fr
Maisonhaute
Claude
C
Maczkowiak
Frédérique
F
Cariou
Marie-Louise
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004064:Digestive System / Q000201:enzymology; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics*; D019143:Evolution, Molecular; D020440:Gene Duplication; D017344:Genes, Insect; D017403:In Situ Hybridization; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014178:Translocation, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D004251:DNA Transposable Elements; D004247:DNA; D000516:alpha-Amylases