A label-free multisensing immunosensor based on imaging ellipsometry.
An immunosensor based on imaging ellipsometry and its potential applications was demonstrated in this paper. It has been proven a fast, reliable, and convenient method to quantify the thickness distribution of protein layers or detect protein concentration in solution. Combined with a protein chip, the immunosensor was able to detect multiple analytes simultaneously without any labeling. Preliminary results demonstrated how this immunosensor could be used to monitor several independent biospecific binding processes in real-time and in situ conditions.
Wang
Zhan Hui
ZH
National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, PR China.
Jin
Gang
G
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003199:Computer Systems; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000276:immunology; D013997:Time Factors
D000906:Antibodies; D012709:Serum Albumin; D012710:Serum Albumin, Bovine