title: Aging is associated with increased T-cell chemokine expression in C57BL/6 mice.
abstract: To better understand the contribution of the chemokine system in immune senescence, we determined the aging effect on CD4+ and CD8+ T-cell chemokine expression by microarray screening and ribonuclease protection assays. Compared with young C57BL/6 mice, freshly isolated CD4+ cells from aged mice express increased level of interferon-gamma-inducible protein 10 (IP-10), macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, regulated upon activation, normal T-cell expressed and secreted (RANTES), and lymphotactin (Ltn). T-cell receptor (TCR)/coreceptor stimulation up-regulates MIP-1alpha, MIP-1beta, and Ltn, and down-regulates IP-10 and RANTES expression in CD4+ T cells. A similar increase in chemokine expression was demonstrated in the CD8+ T cell. Enzyme-linked immunosorbent assays confirmed increased T-cell chemokine protein production in old CD4+ and CD8+ T cells. Finally, supernatant of cultured T cells from old animals caused an enhanced leukocyte chemotaxis response compared with that from young animals, suggesting that the age-related difference in T-cell chemokine expression has an important functional consequence.
authors: Division of Geriatric Medicine and Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, USA.
authors: Jun
authors: J
authors: Chen
authors: Ruran
authors: R
authors: Mo
authors: Pascal A
authors: PA
authors: Lescure
authors: David E
authors: DE
authors: Misek
authors: Samir
authors: S
authors: Hanash
authors: Rosemary
authors: R
authors: Rochford
authors: Monte
authors: M
authors: Hobbs
authors: Raymond L
authors: RL
authors: Yung
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D018925:Chemokines / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012260:Ribonucleases
chemical_list: D018925:Chemokines; D012260:Ribonucleases