Comparative study of eight well-known polyphenolic antioxidants.
Eight antioxidants from five different polyphenolic classes (cinnamic acids, benzoic acids, flavonoids, proanthocyanidins and stilbenes), and the water-soluble vitamin E derivative trolox were examined for their antioxidant activity in-vitro. In addition, the compounds were tested for their cytotoxicity on growing fibroblasts and their inhibition of the classical pathway of the complement system. Procyanidin C1 was shown to be a good scavenger of both DPPH(*) and HO(*), and a strong inhibitor of lipid peroxidation and the classical pathway of the complement system. Consequently, procyanidin C1 was classified as the most promising antioxidant in-vitro of all compounds tested. In contrast, genistein exhibited a very low antioxidant activity in both the lipid peroxidation and the DPPH(*) scavenging assay, a high cytotoxicity and a low complement-inhibiting activity.
Cos
P
P
Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Sciences, University of Antwerp, Belgium. paul.cos@ua.ac.be
Hermans
N
N
Calomme
M
M
Maes
L
L
De Bruyne
T
T
Pieters
L
L
Vlietinck
A J
AJ
Vanden Berghe
D
D
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology; D044946:Biflavonoids / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002839:Chromans / Q000737:chemistry / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D019833:Genistein / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates; D059808:Polyphenols; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013267:Stilbenes / Q000494:pharmacology
D000975:Antioxidants; D001565:Benzoates; D044946:Biflavonoids; D001713:Biphenyl Compounds; D002839:Chromans; D002934:Cinnamates; D005419:Flavonoids; D005609:Free Radicals; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D059808:Polyphenols; D044945:Proanthocyanidins; D013267:Stilbenes; C029010:cinnamic acid; D017665:Hydroxyl Radical; C017674:procyanidin; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl; D019833:Genistein; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid