title: Integration of heterologous genes in several yeast species using vectors containing a Hansenula polymorpha-derived rDNA-targeting element.
abstract: A method that has been successfully used to generate recombinant Hansenula polymorpha strains by transformation with rDNA-targeting vectors was applied in the present study to a range of alternative yeast hosts, using vectors with an H. polymorpha-derived integration sequence. The dimorphic yeast Arxula adeninivorans, which is currently being assessed for heterologous gene expression, was the main focus of the study. As in H. polymorpha, it was possible to co-integrate more than a single plasmid carrying an expressible gene. Additionally, the vectors were examined in two further species, Pichia stipitis and Saccharomyces cerevisiae. Based on these results the design of a 'universal' fungal vector appears to be feasible.
authors: Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany. klabundj@uni-duesseldorf.de
authors: Jens
authors: J
authors: Klabunde
authors: Gotthard
authors: G
authors: Kunze
authors: Gerd
authors: G
authors: Gellissen
authors: Cornelis P
authors: CP
authors: Hollenberg
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004274:DNA, Recombinant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D016681:Genome, Fungal; D016366:Open Reading Frames; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D011994:Recombinant Proteins