Analysis of suppressor T cells induced by donor-specific transfusion (DST): establishment of a human T cell hybridoma producing an antigen-nonspecific suppressor factor.
Formation of suppressor T cells (Ts) induced by donor-specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST-treated patient, which produced an antigen-nonspecific suppressor factor. Post-DST PBL were fused with an azaguanine-resistant mutant of a human T cell leukemia cell line, CCRF-CEM(AG). After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524: 18%-43% suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly augmented (64%-88% MLR suppression). This factor inhibited MLR dose-dependently in an antigen-nonspecific and HLA non-restricted manner. These results indicated that Ts clones could be generated in patients receiving DST and that the immunoregulatory factors produced by activated clones may play a role in the prolongation of renal allograft survival.
Fujiwara
T
T
First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700, Japan.
Sakagami
K
K
Kusaka
S
S
Uda
M
M
Orita
K
K
D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001379:Azathioprine / Q000627:therapeutic use; D001782:Blood Donors; D001803:Blood Transfusion; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
D000941:Antigens; D007166:Immunosuppressive Agents; D004247:DNA; D001379:Azathioprine; D013936:Thymidine