Selection of tomato plants resistant to a local Polish isolate of tomato spotted wilt virus (TSWV).
We found that the Sw-5 gene confers resistance to one of the Polish isolates of tomato spotted wilt virus (TSWV). A series of tomato breeding accessions was analysed along with standards of resistance and susceptibility to TSWV. The presence of the Sw-5 gene was determined using the available PCR marker. Subsequently plants from these accessions were grown in the presence of the TSWV isolate from Poland. Some of them developed severe symptoms of the TSWV disease. Expression of the virus proteins was also assayed in tissues of the investigated plants. We found general agreement between either lack or presence of the disease symptoms, virus proteins and resistance gene. Some observed discrepancies of these data are also discussed. Our results indicate that marker-assisted selection can be used for breeding of the TSWV-resistant tomato in Poland.
Czech
Andrzej S
AS
Department of Plant Physiology and Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland.
Szklarczyk
Marek
M
Gajewski
Zbigniew
Z
Zukowska
Ewa
E
Michalik
Barbara
B
Kobyłko
Tadeusz
T
Strzałka
Kazimierz
K
D004797:Enzyme-Linked Immunosorbent Assay; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012641:Selection, Genetic; D017865:Tospovirus / Q000276:immunology
D010940:Plant Proteins; C419645:Sw-5 protein, Lycopersicon esculentum