Detection of p53 gene mutations by nonisotopic RNase cleavage assay as a predictor of poor prognosis in colorectal cancers.
The present study was undertaken to evaluate p53 gene mutation as a prognostic factor in patients with colorectal cancer. Nonisotopic RNase cleavage assay (NIRCA), recently used for detecting gene mutations, was employed to detect p53 gene mutations in this study. In 15 samples of colorectal tumors, NIRCA was confirmed to be simple, accurate, and thus useful for clinical use, compared with polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP). In another group of 79 cases of colorectal cancer analyzed for p53 gene mutation by using NIRCA, mutations were detected in 58 of 79 (73.4%) cases. Multivariate Cox proportional-hazards analysis showed that p53 gene mutation was a significant prognostic factor in patients with colorectal cancer. Our results showed that NIRCA is a simple and sensitive method, and thus useful for genetic screening of colorectal cancer. Furthermore, our results showed that p53 gene mutation is an independent predictor of poor prognosis in colorectal cancers.
Haseba
Masatoshi
M
First Department of Surgery, Nagasaki University School of Medicine, 1-7-1, Sakamoto Machi, Nagasaki City, Nagasaki 852-8501, Japan. m-haseba@guitar.ocn.ne.jp
Hidaka
Shigekazu
S
Tsuji
Takashi
T
Yano
Hiroshi
H
Komatsu
Hideaki
H
Sawai
Terumitu
T
Yasutake
Toru
T
Nakagoe
Tohru
T
Tagawa
Yutaka
Y
Ayabe
Hiroyoshi
H
D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D016016:Proportional Hazards Models; D012260:Ribonucleases / Q000737:chemistry*; D012680:Sensitivity and Specificity; D016019:Survival Analysis
D012260:Ribonucleases