Preparation of poly(thionine) modified screen-printed carbon electrode and its application to determine NADH in flow injection analysis system.
A poly(thionine) modified screen-printed carbon electrode has been prepared by an electrooxidative polymerization of thionine in neutral phosphate buffer. The modified electrodes are found to give stable and reproducible electrocatlytic responses to NADH and exhibit good stability. Several techniques, including cyclic voltammetry, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM), have been employed to characterize the poly(thionine) film. Further, the modified screen-printed carbon electrode was found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 5-100 microM.
Gao
Qiang
Q
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renmin Street 159, Changchun, Jilin 130022, China.
Cui
Xiaoqiang
X
Yang
Fan
F
Ma
Ying
Y
Yang
Xiurong
X
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D009243:NAD / Q000032:analysis* / Q000737:chemistry*; D010640:Phenothiazines / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D010640:Phenothiazines; D011108:Polymers; D009243:NAD; D002244:Carbon; C009469:thionine