Preparation and characterization of novel coenzyme Q10 nanoparticles engineered from microemulsion precursors.
The purpose of these studies was to prepare and characterize nanoparticles into which Coenzyme Q10 (CoQ10) had been incorporated (CoQ10-NPs) using a simple and potentially scalable method. CoQ10-NPs were prepared by cooling warm microemulsion precursors composed of emulsifying wax, CoQ10, Brij 78, and/or Tween 20. The nanoparticles were lyophilized, and the stability of CoQ10-NPs in both lyophilized form and aqueous suspension was monitored over 7 days. The release of CoQ10 from the nanoparticles was investigated at 37 degrees C. Finally, an in vitro study of the uptake of CoQ10-NPs by mouse macrophage, J774A.1, was completed. The incorporation efficiency of CoQ10 was approximately 74% +/- 5%. Differential Scanning Calorimetry (DSC) showed that the nanoparticle was not a physical mixture of its individual components. The size of the nanoparticles increased over time if stored in aqueous suspension. However, enhanced stability was observed when the nanoparticles were stored at 4 degrees C. Storage in lyophilized form demonstrated the highest stability. The in vitro release profile of CoQ10 from the nanoparticles showed an initial period of rapid release in the first 9 hours followed by a period of slower and extended release. The uptake of CoQ10-NPs by the J774A.1 cells was over 4-fold higher than that of the CoQ10-free nanoparticles (P < .05). In conclusion, CoQ10-NPs with potential application for oral CoQ10 delivery were engineered readily from microemulsion precursors.
Hsu
Cheng-Hsuan
CH
Division of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0082, USA.
Cui
Zhengrong
Z
Mumper
Russell J
RJ
Jay
Michael
M
D000818:Animals; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002460:Cell Line; D002616:Chemical Engineering / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003067:Coenzymes; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry*; D005612:Freeze Drying / Q000379:methods; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D012995:Solubility; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism*
D003067:Coenzymes; D004655:Emulsions; D014451:Ubiquinone; C024989:coenzyme Q10