Structure of the polysaccharide isolated from the sheath of Sphaerotilus natans.
A polysaccharide was isolated from the sheath of a sheathed bacterium, Sphaerotilus natans. The sheath polysaccharide (SPS) was composed of D-glucose and D-(N-acetyl)galactosamine in molar ratios of 1:4. Methylation linkage analysis revealed the presence of the residues of 4-linked glucose, 4-linked (N-acetyl)galactosamine, and 3-linked (N-acetyl)galactosamine in molar ratios of 1:3:1. The oligomer of SPS was prepared with an SPS-specific degrading enzyme from a sheath-degrading bacterium, Paenibacillus koleovorans. The oligomer was derivatized and subjected to fast atom bombardment-mass spectrometry to investigate the monosaccharide sequence of SPS. The structure of SPS was confirmed by nuclear magnetic resonance. The resulting data showed that SPS is a straight-chained basic polysaccharide constructed of a pentasaccharide repeating unit.
Takeda
Minoru
M
Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya, Yokohama 240-8501, Japan. mtake@ynu.ac.jp
Nakamori
Tomonari
T
Hatta
Miwa
M
Yamada
Hiroki
H
Koizumi
Jun-ichi
J
D000116:Acetylgalactosamine / Q000032:analysis / Q000737:chemistry; D001419:Bacteria / Q000201:enzymology; D020562:Betaproteobacteria / Q000737:chemistry* / Q000502:physiology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004798:Enzymes / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004798:Enzymes; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D005947:Glucose; D000116:Acetylgalactosamine