title: Gene expression of alpha-endosulfine in the rat brain: correlative changes with aging, learning and stress.
abstract: Endosulfine (EDSF) belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family and was first isolated from ovine brain as a possible endogenous ligand for sulfonylurea receptors. To explore its involvement in brain functions, we investigated regional distribution of alpha-EDSF gene expression in the rat brain, and its regulation under physiological and pathological conditions. The majority of alpha-EDSF gene was expressed in the pyramidal neurons, which represent the principal excitatory neurons in various brain regions. Down-regulation of alpha-EDSF mRNA was detected in the rat hippocampus during long-term memory consolidation following a spatial learning experience, whereas swimming-related stress caused persistent up-regulation of alpha-EDSF gene expression in several brain regions. These changes, however, were absent from brains of diabetic rats that were subjected to the same behavioral treatments. Intracerebroventricular injection of streptozocin with a toxic dose induced severe learning deficits and brain structure alteration accompanied by a massive increase of alpha-EDSF mRNA in the somatosensory cortex. These results suggest that alpha-EDSF gene expression is differentially regulated by distinct brain processes involving excitatory neuronal activities.
authors: Blanchette Rockefeller Neurosciences Institute, 9601 Medical Center Drive, Rockville, MD 20850-3332, USA.
authors: Jingtao
authors: J
authors: Dou
authors: Changhai
authors: C
authors: Cui
authors: Franck
authors: F
authors: Dufour
authors: Daniel L
authors: DL
authors: Alkon
authors: Wei-Qin
authors: WQ
authors: Zhao
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism; D029721:Drosophila Proteins; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017403:In Situ Hybridization; D007276:Injections, Intraventricular; D036341:Intercellular Signaling Peptides and Proteins; D007858:Learning / Q000502:physiology*; D008297:Male; D010455:Peptides / Q000235:genetics*; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000187:drug effects / Q000378:metabolism; D013311:Streptozocin / Q000008:administration & dosage; D013312:Stress, Physiological / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; C483180:endos protein, Drosophila; C075663:endosulfine; D013311:Streptozocin