FUM9 is required for C-5 hydroxylation of fumonisins and complements the meitotically defined Fum3 locus in Gibberella moniliformis.
Deletion of the Gibberella moniliformis FUM9 gene resulted in mutants that produce only fumonisins that lack a C-5 hydroxyl group. This phenotype is identical to that of previously described mutants with defective alleles at the meiotically defined Fum3 locus. Transformation with a wild-type FUM9 gene into a Fum3-defective mutant restored wild-type fumonisin production. These results indicate that the FUM9 protein catalyzes the C-5 hydroxylation of fumonisins and that FUM9 and the Fum3 locus are the same gene.
Butchko
Robert A E
RA
National Center for Agricultural Resource Utilization, USDA Agricultural Research Service, Peoria, Illinois 61604, USA.
Plattner
Ronald D
RD
Proctor
Robert H
RH
D037341:Fumonisins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D005874:Gibberella / Q000235:genetics* / Q000378:metabolism; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D009183:Mycotoxins / Q000378:metabolism; D014170:Transformation, Genetic; D003313:Zea mays / Q000382:microbiology
D037341:Fumonisins; D005656:Fungal Proteins; D009183:Mycotoxins; D006899:Mixed Function Oxygenases