title: Chromosome number variation in somatic hybrids between transgenic tomato (Lycopersicon esculentum) and Solanum lycopersicoides.
abstract: Leaf mesophyll protoplasts of Lycopersicon esculentum were fused with suspension-culture-derived protoplasts of Solanum lycopersicoides by a PEG treatment. Both species have the same chromosome number (2n = 2x = 24). The hybrid calli were selected using the full selection method - kanamycin resistance and culture conditions critical for L. esculentum protoplast divisions. The genomic in situ hybridization analyses indicated a hypo- and hypertetraploid character of the hybrid plant with a majority of S. lycopersicoides chromosomes and a variation in chromosome number from 46 to 53. The hybrids contained a transgene derived from L. esculentum, as shown by Southern blot hybridization and PCR analyses. Their mitochondria were derived from the wild species, S. lycopersicoides. More than 60 regenerated plants were transferred into the greenhouse. They grew very slowly and were not able to flower for almost one year. The main morphological characters of the hybrids included a single shoot and small, dark-green leaves with strongly wrinkled blades. The reasons for nuclear genome asymmetry between hybrids and the possibilities of using them in a genetic and breeding programme are discussed in this paper.
authors: Cell Biology Department, Maria Curie-Skłodowska University, Lublin, Poland.
authors: Mariola
authors: M
authors: Kulawiec
authors: Norikazu
authors: N
authors: Tagashira
authors: Wojciech
authors: W
authors: Plader
authors: Grzegorz
authors: G
authors: Bartoszewski
authors: Dominik
authors: D
authors: Kuć
authors: Renata
authors: R
authors: Sniezko
authors: Stefan
authors: S
authors: Malepszy
mesh_terms: D016960:Agrobacterium tumefaciens; D015139:Blotting, Southern; D032461:Chromosomes, Plant; D005822:Genetic Vectors; D006824:Hybridization, Genetic; D018551:Solanum lycopersicum / Q000235:genetics*; D030821:Plants, Genetically Modified; D011523:Protoplasts; D014162:Transfection