title: Differential expression of isoproterenol-induced salivary polypeptides in two mouse strains that are congenic for the H-2 histocompatibility gene complex.
abstract: Two inbred mouse strains, A/Snell and A.Swiss, which were produced as congenic with regard to the H-2 histocompatibility gene complex, are homozygous for two different groups of isoproterenol-induced salivary polypeptides (IISP). These polypeptides, which have been considered as markers of the hypertrophic growth of the parotid acinar cells, are members of the complex family of salivary proline-rich proteins (PRP) on the basis of both their massive accumulation in the parotid acinar cells in response to chronic isoproterenol, secretory character, high solubility in trichloroacetic acid and metachromatic staining by Coomassie blue. IISP expressed in both mouse strains were identified by unidimensional SDS-polyacrylamide electrophoresis and Coomassie blue staining both in parotid gland homogenates and in whole salivas obtained from mice repeatedly stimulated at 24-h intervals with isoproterenol. Parotid glands from 40 mice (20 A/Snell and 20 A.Swiss) and salivas from 270 mice (200 A/Snell and 70 A.Swiss) were analyzed. One of the congenic strains (A/Snell) expressed five IISP (Mr 65, 61, 51.5, 38, and 37 kDa) and the other strain (A.Swiss) expressed six IISP (Mr 59, 57, 54.5, 46, 36, and 34 kDa). No inter-individual intra-strain variations were observed, thus defining strain-associated patterns of IISP (PRP).
authors: Cellular and Molecular Biology Unit, Institute for Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 7, Chile. rlopez@machi.med.uchile.cl
authors: Remigio O
authors: RO
authors: López Solís
authors: Ulrike Kemmerling
authors: UK
authors: Weis
authors: Alicia Ramos
authors: AR
authors: Ceballos
authors: Gustavo Hoecker
authors: GH
authors: Salas
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005805:Genes, MHC Class I; D006720:Homozygote; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008815:Mice, Inbred Strains; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D055232:Proline-Rich Protein Domains; D012463:Saliva / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D010455:Peptides; D012471:Salivary Proteins and Peptides; D007545:Isoproterenol