SetB: an integral membrane protein that affects chromosome segregation in Escherichia coli.
SetB was identified as a high-copy suppressor of the partition defect of a mutation in parC, encoding one of the subunits of topoisomerase IV. Deletion of this integral inner membrane protein causes a delay in chromosome segregation, whereas its overproduction causes nucleoid disintegration and stretching, leading to a cell division defect. setB deletion mutants also exhibit a synthetic phenotype when combined with mutations that delete the C-terminal motor domain of the septal ring protein FtsK. SetB localizes in the cell as a helix and interacts with MreB, the bacterial actin homologue, which also forms a helix. These observations suggest that there may be a link between chromosome segregation and cellular infrastructure.
Espeli
Olivier
O
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Nurse
Pearl
P
Levine
Cindy
C
Lee
Chong
C
Marians
Kenneth J
KJ
D002455:Cell Division; D020090:Chromosome Segregation / Q000502:physiology*; D002876:Chromosomes, Bacterial / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D009004:Monosaccharide Transport Proteins / Q000502:physiology*
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; C119764:setB protein, E coli