Detection of autoantibodies in the serum of primary hepatocarcinoma patients.
OBJECTIVE
To study the significance of detecting autoantibodies in primary hepatocarcinoma (PHC) patients.


METHODS
Autoantibodies were detected by indirect immunofluorescence assay. Antigens and antibodies of HBV were determined by enzyme immune assay. Antibody to HCV IgG was detected by enzyme-linked immunoabsorbent assay.


RESULTS
The positive rate of autoantibody was 27.3% (38/139) in 139 PHC patients. The main type of autoantibodies in PHC was anti-nuclear antibody (36/38, 94.7%), others included anti-smooth muscle antibody(2/38, 5.3%), anti-mitochondria antibody (1/38, 2.6%), anti-midbody antibody (1/38, 2.6%, and anti-liver cell membrane antibody (2/38, 5.3%).


CONCLUSIONS
Detecting autoantibodies in PHC patients is of significance in studying the mechanism of autoimmune reaction and etiology in PHC. The diversity of autoantibodies might result from a wide variety of etiological factors involved in PHC development, and from a wide variety of overexpressed or mutated proteins involved in repeated cycles of necrosis and regeneration in hepatocarcinoma development.
Fang
Fang
F
Department of Clinical Laboratory, Shanghai Eastern Hepatobiliary Hospital, Shanghai 200418, China.
Wang
Hua-Liang
HL
Ye
Ping
P
Deng
Hai-Lin
HL
Dong
Gui-Ling
GL
Ma
Li-Ling
LL
Wang
Jian
J
D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000097:blood*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006510:Hepatitis B Antibodies / Q000097:blood; D019694:Hepatitis B, Chronic / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000453:epidemiology* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology; D009130:Muscle, Smooth / Q000276:immunology; D016036:Seroepidemiologic Studies
D000974:Antibodies, Antinuclear; D006510:Hepatitis B Antibodies