Application of Bacillus anthracis PCR to simulated clinical samples.
We evaluated PCR for the detection of Bacillus anthracis DNA from simulated clinical specimens relevant for the microbiological diagnosis of anthrax or exposure to B. anthracis spores. In simulated blood specimens, the lowest limit of detection was 400 CFU per mL of blood, which may be sufficient for samples from patients with septic anthrax. Screening nasal swabs by PCR may not be sensitive enough to rule out dangerous exposure to anthrax spores, as a minimum of 2000 spores per sample was required for detectable amplification. As spores survived some standard DNA purification methods, special attention should be paid to laboratory safety when preparing samples possibly containing live spores.
Rantakokko-Jalava
K
K
Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland. kaisu.rantakokko@utu.fi
Viljanen
M K
MK
D000881:Anthrax / Q000097:blood / Q000382:microbiology*; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009297:Nasal Mucosa / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial; D013234:Stem Cells
D004269:DNA, Bacterial