Whole-comparative genomic hybridization (W-CGH): 1. The quick overview of repetitive DNA sequences on a genome.
We present a technique (W-CGH) based on Comparative Genomic Hybridization (CGH), but using whole DNA probes, which permits the identification of chromosomal polymorphisms related to highly repetitive DNA sequences that exist between the two genomes compared. The procedure employs two differently colored whole DNA probes from two different individuals that are mixed and hybridized to metaphase chromosomes. The method provides a simple way to map whole genome differences for highly repetitive DNA sequences between two individuals, since it does not require chromosome-specific probes.
Pita
Miguel
M
Unidad de Genética, Departamento de Biologia, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.pita@uam.es
Fernandez
José Luis
JL
Gosálvez
Jaime
J
D001483:Base Sequence; D004247:DNA / Q000648:ultrastructure*; D015342:DNA Probes / Q000235:genetics*; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004247:DNA