title: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.
abstract: Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.
authors: Institute of Molecular Genetics, AS CR, Vídenská 1083, 142 20 Praha 4, Czech Republic.
authors: Nadezda
authors: N
authors: Brdicková
authors: Tomás
authors: T
authors: Brdicka
authors: Pavla
authors: P
authors: Angelisová
authors: Ondrej
authors: O
authors: Horváth
authors: Jiri
authors: J
authors: Spicka
authors: Ivan
authors: I
authors: Hilgert
authors: Jan
authors: J
authors: Paces
authors: Luca
authors: L
authors: Simeoni
authors: Stefanie
authors: S
authors: Kliche
authors: Camilla
authors: C
authors: Merten
authors: Burkhart
authors: B
authors: Schraven
authors: Václav
authors: V
authors: Horejsí
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D000081247:CSK Tyrosine-Protein Kinase; D018076:DNA, Complementary; D030562:Databases, Protein; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D021962:Membrane Microdomains / Q000276:immunology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D019061:src-Family Kinases
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D015704:CD4 Antigens; D016827:CD8 Antigens; D018076:DNA, Complementary; C480489:Lck-interacting protein, mouse; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019061:src-Family Kinases; C000631346:CSK protein, human