title: [Phenotypical analysis of effector cells on nonspecific cancer cell therapy].
abstract: In the present study, we examined the contributions of lymphocyte subpopulations in lymphokine activated killer (LAK) activity. LAK cells prepared from peripheral blood mononuclear cells (PBMC) of healthy donors showed highly cytotoxic activities against target tumor cells. When CD16 and CD56 positive cells in LAK cells were depleted by magnetic cell sorting, their cytotoxic activities were dramatically decreased. In contrast, little change was observed by the depletion of CD3 positive cells, suggesting that CD16 and/or CD56 positive populations, but not CD3 positive populations, including natural killer (NK) cells are the major cell types involved in LAK activity. Indeed, NK-enriched LAK cells prepared by culturing PBMC with IL-2 and OK-432 showed a more potent LAK activity than conventional LAK cells and CD3-activated T cells. These results suggest that selective expansion and activation of CD16 and CD56 positive cells in LAK cells is a useful strategies to improve their anti-tumor potential in nonspecific immunotherapy, and possibly in combination therapy with other target immunotherapies as well.
authors: Dept. of Gastroenterological Surgery, Tokyo Women's Medical University.
authors: Toshimi
authors: T
authors: Sudo
authors: Yasunobu
authors: Y
authors: Kobayashi
authors: Norimasa
authors: N
authors: Matsushita
authors: Kouichi
authors: K
authors: Shimizu
authors: Masanobu
authors: M
authors: Nakao
authors: Yoshinori
authors: Y
authors: Tanaka
authors: Keishi
authors: K
authors: Tanigawa
authors: Atsushi
authors: A
authors: Aruga
mesh_terms: D017252:CD3 Complex / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology; D020014:K562 Cells; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology / Q000637:transplantation*; D007694:Killer Cells, Natural / Q000276:immunology; D010844:Picibanil / Q000494:pharmacology
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D007376:Interleukin-2; D010844:Picibanil