Antithrombic and anti-atherogenic effects of partially defatted flaxseed meal using a laser-induced thrombosis test in apolipoprotein E and low-density lipoprotein receptor deficient mice.
Atherothrombosis can be regarded as a 'life-style related disease' of which diet is one of the important risk factors. The prophylactic effect of partially defatted flaxseed meal (PDFM) on atherothrombosis has not yet been studied. The aim of this study was to assess the effect of PDFM and a lignan from flaxseed, secoisolariciresinol diglucoside (SDG), on thrombosis and atherogenesis. An earlier developed test, the quantitative assessment of laser-induced thrombus formation in the carotid artery of apolipoprotein E and low-density lipoprotein receptor deficient mice was used in this study. Thrombotic and atherosclerotic status was assessed in mice kept on a high-fat diet for 8 weeks (40% in energy). The diet contained 0.05% cholesterol alone (control) or the same cholesterol with added PDFM (5% w/w; 8.3 g/kg body weight per day) or SDG (0.06% w/w; 100 mg/kg body weight per day). PDFM showed antithrombotic (P < 0.01) and anti-atherogenic effect (P < 0.01). SDG did not affect either atherogenesis or thrombosis. This study suggests that dietary intake of PDFM can be beneficial in reducing the risk of high-fat-induced atherothrombosis.
Sano
Takashi
T
Laboratory of Physiology, Faculty of Nutrition, and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan. takashi_sano@mail.yoshihara.co.jp
Oda
Etsuko
E
Yamashita
Takatoshi
T
Shiramasa
Hiroshi
H
Ijiri
Yoshinobu
Y
Yamashita
Tsutomu
T
Yamamoto
Junichiro
J
D000818:Animals; D001161:Arteriosclerosis / Q000517:prevention & control*; D002072:Butylene Glycols / Q000737:chemistry / Q000494:pharmacology; D004032:Diet; D004195:Disease Models, Animal; D019597:Flax / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D007834:Lasers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D013927:Thrombosis / Q000517:prevention & control*
D002072:Butylene Glycols; D005960:Glucosides; C090142:secoisolariciresinol diglucoside