[Expression of androgen receptor isoforms in normal human prostate].
OBJECTIVE
To investigate the expression of androgen receptor (AR) isoforms in normal human prostate.


METHODS
Fourteen normal prostatic specimens from donors, aged 25 on average (21-28 yr), were analyzed by high resolution isoelectric focusing (IEF). The expression of AR isoforms was demonstrated in all 14 normal human prostatic tissues.


RESULTS
Four types of AR isoforms were detected with isoelectric point value at 6.5, 6.0, 5.8 and 5.3 in 14 prostatic specimens. Binding of 3H-dihydrotestosterone (DHT) to these four AR isoforms was inhibited by the addition of 100-fold excess of DHT and testosterone. No effect of progesterone, estradiol and diethylstilbestrol on tritiated hormone binding was observed.


CONCLUSIONS
There are four AR isoforms in normal human prostate. The expression of AR isoforms is different from one another.
Xia
Shu-Jie
SJ
Department of Urology, Shanghai First People's Hospital, Jiaotong University, Shanghai 200080, China.
Hao
Gang-Yue
GY
Wen
Wei
W
Zhang
Jie
J
Li
Wei-Guo
WG
Zhu
Yi-Yong
YY
Tang
Xiao-Da
XD
D000328:Adult; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D011467:Prostate / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis; D011944:Receptors, Androgen / Q000096:biosynthesis*
D020033:Protein Isoforms; D011944:Receptors, Androgen