Assignment of human sprouty 4 gene to chromosome segment 5q32 approximately 33 and analysis of its pattern of expression.
The human sprouty 4 (SPRY4) gene was localized to chromosome band 5q32 approximately 33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPRY4 was expressed in all tested tissues to different levels.
Liu
Hua
H
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China. ymmao@fudan.edu.cn
Chen
Jin-Zhong
JZ
Gu
Shao-Hua
SH
Dai
Jian-Liang
JL
Zhao
En-Pang
EP
Huang
Lu
L
Xu
Wang-Xiang
WX
Xie
Yi
Y
Mao
Yu-Min
YM
D015152:Blotting, Northern; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D021101:Radiation Hybrid Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; C499038:SPRY4 protein, human