title: The amino and carboxyl termini of perilipin a facilitate the storage of triacylglycerols.
abstract: Perilipin A is the most abundant lipid droplet-associated protein in adipocytes and serves important functions in regulating triacylglycerol levels by reducing rates of basal lipolysis and facilitating hormonally stimulated lipolysis. We have previously shown that the central region of perilipin A targets and anchors it to lipid droplets, at least in part via three moderately hydrophobic sequences that embed the protein into the hydrophobic core of the droplet. The current study examines the roles of the amino and carboxyl termini of perilipin A in facilitating triacylglycerol storage. Amino- and carboxyl-terminal truncation mutations of mouse perilipin A were stably expressed in 3T3-L1 preadipocytes, which lack perilipins. Triacylglycerol content of the cells was quantified as a measure of perilipin function and was compared with that of cells expressing full-length perilipin A or control cells lacking perilipins. The amino-terminal sequence between amino acids 122 and 222, including four 10-11-amino acid sequences predicted to form amphipathic beta-strands and a consensus site for cAMP-dependent protein kinase, and the carboxyl terminus of 112 amino acids that is unique to perilipin A were critical to facilitate triacylglycerol storage. The precocious expression of full-length perilipin A in 3T3-L1 preadipocytes aided more rapid storage of triacylglycerol during adipose differentiation. By contrast, the expression of highly truncated amino- or carboxyl-terminal mutations of perilipin failed to serve a dominant negative function in lowering triacylglycerol storage during adipose differentiation. We conclude that the amino and carboxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage.
authors: Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA.
authors: Anne
authors: A
authors: Garcia
authors: Vidya
authors: V
authors: Subramanian
authors: Anna
authors: A
authors: Sekowski
authors: Sucharita
authors: S
authors: Bhattacharyya
authors: Martha W
authors: MW
authors: Love
authors: Dawn L
authors: DL
authors: Brasaemle
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins; D002454:Cell Differentiation; D002460:Cell Line; D017124:Conserved Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D016296:Mutagenesis; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger; D013234:Stem Cells / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D010446:Peptide Fragments; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D014280:Triglycerides; D017868:Cyclic AMP-Dependent Protein Kinases