Investigation and optimization of the factors influencing sorghum protein extraction.
To optimize the extraction of sorghum proteins, several variables were examined: sample-to-solvent ratio, detergent type and concentration, reducing agent type and concentration, extraction time, and buffer pH and concentration. Samples were quantified and characterized by RP-HPLC, FZCE, and nitrogen analysis. These studies revealed that pH, detergent type, reducing agent type, and sample-to-solvent ratio all had significant effects on the levels of protein extracted. Increasing SDS concentration (2%) and solvent-to-flour ratio (20:1) with multiple 5 min extracts reduced extraction time by 35-80% while still extracting the same levels of total protein relative to the control methodology. Reproducibility using the multiple extractions was found to be excellent with relative standard deviations of <2% for consecutive extractions.
Park
Seok-Ho
SH
Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506, USA.
Bean
Scott Roger
SR
D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D003902:Detergents; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D019163:Reducing Agents; D015203:Reproducibility of Results; D012997:Solvents; D045868:Sorghum / Q000737:chemistry*
D003902:Detergents; D010936:Plant Extracts; D010940:Plant Proteins; D019163:Reducing Agents; D012997:Solvents; C080615:kafirin protein, Sorghum bicolor