Human bone marrow stromal cell responses on electrospun silk fibroin mats.
Fibers with nanoscale diameters provide benefits due to high surface area for biomaterial scaffolds. In this study electrospun silk fibroin-based fibers with average diameter 700+/-50 nm were prepared from aqueous regenerated silkworm silk solutions. Adhesion, spreading and proliferation of human bone marrow stromal cells (BMSCs) on these silk matrices was studied. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices supported BMSC attachment and proliferation over 14 days in culture similar to native silk fibroin (approximately 15 microm fiber diameter) matrices. The ability of electrospun silk matrices to support BMSC attachment, spreading and growth in vitro, combined with a biocompatibility and biodegradable properties of the silk protein matrix, suggest potential use of these biomaterial matrices as scaffolds for tissue engineering.
Jin
Hyoung-Joon
HJ
Department of Chemical & Biological Engineering, Biomedical Engineering, Bioengineering Center, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
Chen
Jingsong
J
Karageorgiou
Vassilis
V
Altman
Gregory H
GH
Kaplan
David L
DL
D000293:Adolescent; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D012831:Bombyx / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure*; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry*; D008420:Manufactured Materials / Q000032:analysis; D008422:Materials Testing; D008567:Membranes, Artificial; D047011:Silk; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013784:Textiles; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D019476:Insect Proteins; D008567:Membranes, Artificial; D047011:Silk; D005349:Fibroins