title: Microsatellite-AFLP for genetic mapping of complex polyploids.
abstract: In spite of the economical relevance of polyploid crops, genetic mapping of these species has been relatively overlooked. This is because of intrinsic difficulties such as the uncertainty of the chromosome behavior at meiosis I and the need for very large segregating populations. An important, yet underestimated issue, in mapping polyploids is the choice of the molecular marker system. An ideal molecular marker system for polyploid mapping should maximize the percentage of single dose markers (SDMs) detected and the possibility of recognizing allelic markers. In the present work, the marker index for genetic mapping (MIgm) of M-AFLP is compared with that of AFLP and SAMPL. M-AFLPs have the highest MIgm values (22 vs. 18.5 of SAMPL and 9.83 of AFLP) mostly because of their high power to detect polymorphism. Owing to their prevalent codominant inheritance, it is proposed that M-AFLP can be used for the preliminary identification of hom(e)ologous groups.
authors: Department of Plant Biology and Agro-environmental Biotechnology, University of Perugia, Italy.
authors: Emidio
authors: E
authors: Albertini
authors: Andrea
authors: A
authors: Porceddu
authors: Gianpiero
authors: G
authors: Marconi
authors: Gianni
authors: G
authors: Barcaccia
authors: Luca
authors: L
authors: Pallottini
authors: Mario
authors: M
authors: Falcinelli
mesh_terms: D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D008126:Lod Score; D018895:Microsatellite Repeats; D011123:Polyploidy; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers