title: Expression of transforming growth factor beta1 in nerve fibers is related to dysmenorrhea and laparoscopic appearance of endometriotic implants.
abstract: OBJECTIVE
  To quantify the expression of transforming growth factor beta1 in nerve fibers in endometriotic lesions and to correlate it with dysmenorrhea and appearance of endometriotic implants.
  
  
  METHODS
  Prospective comparative study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Peritoneal endometriotic specimens obtained from 35 patients diagnosed with endometriosis were compared with biopsies of normal peritoneum from 10 patients without endometriosis.
  
  
  METHODS
  Endometriosis-associated dysmenorrhea for each patient was evaluated before surgery using a 10-point visual analog scale, which was followed by a laparoscopic staging of the patient's endometriosis.
  
  
  METHODS
  Immunohistochemical analysis of the peritoneal endometriotic specimens evaluated the maximal intensity of staining (INTMMAX) of TGFbeta1, defined as higher staining intensity found within a selected structure.
  
  
  RESULTS
  When the nerve fibers of endometriotic lesions were compared with those of normal peritoneum, statistically significant differences were found in the INTMMAX of TGFbeta1. Greater TGFbeta1 INTMMAX was found in red lesions and deep endometriotic foci than in black lesions and normal peritoneum. A statistically significant relationship was found between the TGFbeta1 INTMMAX score and dysmenorrhea; a relationship also was found to the color of the lesions.
  
  
  CONCLUSIONS
  The physical appearance of endometriotic implants and the severity of dysmenorrhea appear to be related to the expression of TGFbeta1 in nerve fibers.
authors: Department of Gynecology, Hôtel-Dieu, Polyclinique, CHU, Clermont-Ferrand, France.
authors: Stefano
authors: S
authors: Tamburro
authors: Michel
authors: M
authors: Canis
authors: Eliane
authors: E
authors: Albuisson
authors: Pierre
authors: P
authors: Dechelotte
authors: Claude
authors: C
authors: Darcha
authors: Gérard
authors: G
authors: Mage
mesh_terms: D000328:Adult; D001706:Biopsy; D003116:Color; D004412:Dysmenorrhea / Q000378:metabolism / Q000503:physiopathology*; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology*; D004717:Endometrium / Q000294:innervation / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015907:Hysteroscopy; D007150:Immunohistochemistry / Q000379:methods; D007247:Infertility, Female / Q000378:metabolism; D015999:Multivariate Analysis; D009412:Nerve Fibers / Q000378:metabolism*; D017699:Pelvic Pain / Q000378:metabolism; D010537:Peritoneum / Q000294:innervation / Q000473:pathology; D011446:Prospective Studies; D012372:ROC Curve; D012720:Severity of Illness Index; D013194:Staining and Labeling; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
chemical_list: C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1