title: Complete nucleotide sequence and phylogenetic analyses of hepatitis B virus isolated from two pileated gibbons.
abstract: We analyzed full-length sequence of hepatitis B virus (HBV) recovered from two pileated gibbons (Hylobates pileatus) originally born in East Asia. Two animals possessed a viral genome of 3182 nt in length with a 33 nt deletion in the pre-S1 region, and designated HBV PG-Makiko and HBV PG-Yohko, respectively. Both sequences had 65-90% similarity to type A-G of human HBV isolates. Phylogenetic analysis demonstrated that both isolates were distinct from the human and other nonhuman primate HBV isolates, but could be classified into gibbon isolates that were previously reported by others. Small spherical and tubular particles and large particles with outer envelopes were observed in the serum under immunoelectron microscopic examination. By immunohistochemical staining, HBsAg and HBcAg were detected in the cytoplasm and nuclei of hepatocytes, respectively. Our results suggested that HBV found in these animals is indigenous to their respective hosts and not recent acquisitions from human.
authors: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
authors: Naoto
authors: N
authors: Aiba
authors: Hiroyuki
authors: H
authors: Nishimura
authors: Yasuyuki
authors: Y
authors: Arakawa
authors: Kenji
authors: K
authors: Abe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006509:Hepatitis B / Q000662:veterinary / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D006922:Hylobates / Q000821:virology*; D008099:Liver / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014766:Viremia / Q000662:veterinary / Q000821:virology
chemical_list: D004279:DNA, Viral