Isolation and characterization of a thermophilic bacterium, Geobacillus thermocatenulatus, degrading nylon 12 and nylon 66.
A thermophilic bacterium, identified as a neighboring species to Geobacillus thermocatenulatus, having a growth optimum at 55 degrees C and, capable of degrading nylon 12, was isolated from soil by enrichment culture technique at 60 degrees C. At this temperature, the strain grew on 5 g nylon 12 l(-1) with a decrease in its molecular weight from 41000 to 11000 over 20 d. The degradation was assumed to be due to endogenous hydrolysis of amide bond in nylon 12. The strain degraded also nylon 66 with a decrease in its molecular weight from 43000 to 17000 in 20 d at 60 degrees C. Nylon 6 was not degraded.
Tomita
Kosuke
K
College of Engineering, Kanto Gakuin University, 1-50-1 Mutsuura-higashi, Kanazawa-ku, Yokohama 236-8501, Japan. tomitak@kanto-gakuin.ac.jp
Ikeda
Noritoshi
N
Ueno
Ayumi
A
D001406:Bacillaceae / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*; D001673:Biodegradation, Environmental; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D007220:Industrial Waste / Q000517:prevention & control; D008970:Molecular Weight; D009757:Nylons / Q000737:chemistry* / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013045:Species Specificity
D007220:Industrial Waste; D009757:Nylons; D012989:Soil Pollutants; C036222:nylon 12