In vitro and in vivo interactions between the hepatitis B virus protein P22 and the cellular protein gC1qR.
gC1qR, a mitochondrial matrix protein, was identified as the main cellular partner of the hepatitis B virus P22 protein. We demonstrated by immunofluorescence studies that some P22 molecules were colocalized with the endogenous gC1qR in both the cytoplasm and the nucleus but never in the mitochondria. We also showed that the last 34 amino acids of P22 were involved in the association with gC1qR.
Lainé
S
S
Laboratoire de Génétique et Biologie Cellulaire, Université de Versailles St. Quentin, Versailles, France.
Thouard
A
A
Derancourt
J
J
Kress
M
M
Sitterlin
D
D
Rossignol
J-M
JM
D000595:Amino Acid Sequence; D002352:Carrier Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011951:Receptors, Complement / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
C087299:C1QBP protein, human; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; D014764:Viral Proteins; C032402:complement 1q receptor