The calcium sensor CBL1 integrates plant responses to abiotic stresses.
Calcium ions represent both an integrative signal and an important convergence point of many disparate signaling pathways. Calcium-binding proteins, like calcineurin B-like (CBL) proteins, have been implicated as important relays in calcium signaling. Here, we report the in vivo study of CBL1 function in Arabidopsis. Analyses of loss-of-function as well as CBL1-overexpressing lines indicate a crucial function of this calcium sensor protein in abiotic stress responses. Mutation of CBL1 impairs plant responses to drought and salt stresses and affects gene expression of cold-regulated genes, but does not affect abscisic acid (ABA) responsiveness. Conversely, overexpression of CBL1 reduces transpirational water loss and induces the expression of early stress-responsive transcription factors and stress adaptation genes in non-stressed plants. Together, our data indicate that the calcium sensor protein CBL1 may constitute an integrative node in plant responses to abiotic stimuli and contributes to the regulation of early stress-related transcription factors of the C-Repeat-Binding Factor/dehydration-responsive element (CBF/DREB) type.
Albrecht
Verónica
V
Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany.
Weinl
Stefan
S
Blazevic
Dragica
D
D'Angelo
Cecilia
C
Batistic
Oliver
O
Kolukisaoglu
Uner
U
Bock
Ralph
R
Schulz
Burkhard
B
Harter
Klaus
K
Kudla
Jörg
J
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004190:Disasters; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D018526:Plant Transpiration / Q000187:drug effects; D030821:Plants, Genetically Modified; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D002118:Calcium; D002135:Calcium-Binding Proteins; D004269:DNA, Bacterial; D012965:Sodium Chloride; D014157:Transcription Factors; C484078:CBL10 protein, Arabidopsis; C484079:CBL3 protein, Arabidopsis; C514582:CBL9 protein, Arabidopsis; C036483:T-DNA; C483419:calcineurin B-like protein 1, Arabidopsis; C480758:At5g55990 protein, Arabidopsis