Measurement of skeletal muscle mass in Duchenne muscular dystrophy: use of 24-h creatinine excretion.
Creatinine concentration in 24-h urine has been proposed as an indirect measure of body skeletal muscle mass (SMM). We attempted to correlate urinary creatinine levels with SMM in eight patients with Duchenne muscular dystrophy, a progressive disease in which the degree of muscle wasting parallels the rate of progression. Magnetic resonance imaging and a newly developed protocol for image analysis were used for the measurement of SMM. The patients ate a creatine-free diet for the week before urine collection. Creatinine was measured with an enzymatic-colorimetric method. Mean (+/-SD) SMM value was 5.4+/-2.5 kg and urine creatinine levels 205.8+/-96.4 mg/day. Daily urinary creatinine excretion did not correlate with SMM. The simple creatinine determination in urine cannot predict SMM in Duchenne muscular dystrophy.
Franciotta
D
D
IRCCS Foundation, Neurological Institute C Mondino, Pavia, Italy.
Zanardi
M C
MC
Albertotti
L
L
Orcesi
S
S
Berardinelli
A
A
Pichiecchio
A
A
Uggetti
C
C
Tagliabue
A
A
D015415:Biomarkers / Q000652:urine; D001827:Body Height; D001835:Body Weight; D002648:Child; D003404:Creatinine / Q000652:urine*; D006801:Humans; D018482:Muscle, Skeletal / Q000033:anatomy & histology*; D020388:Muscular Dystrophy, Duchenne / Q000503:physiopathology*; D012044:Regression Analysis; D015203:Reproducibility of Results
D015415:Biomarkers; D003404:Creatinine