title: hZimp10 is an androgen receptor co-activator and forms a complex with SUMO-1 at replication foci.
abstract: The androgen receptor (AR) plays a central role in male sexual development and in normal and malignant prostate cell growth and survival. It has been shown that transcriptional activation of AR is regulated through interaction with various co-factors. Here we identify a novel PIAS-like protein, hZimp10, as an AR-interacting protein. The transactivation domain (TAD) of AR and the central region of hZimp10 were found to be responsible for the interaction. A strong intrinsic transactivation domain was identified in the C-terminal, proline-rich region of hZimp10. Endogenous AR and hZimp10 proteins were co-stained in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, hZimp10 augmented the transcriptional activity of AR. Moreover, hZimp10 co-localized with AR and SUMO-1 at replication foci throughout S phase, and it was capable of enhancing sumoylation of AR in vivo. Studies using sumoylation deficient AR mutants suggested that the augmentation of AR activity by hZimp10 is dependent on the sumoylation of the receptor. Taken together, these data demonstrate that hZimp10 is a novel AR co-regulator.
authors: Department of Urology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors: Manju
authors: M
authors: Sharma
authors: Xiaoyu
authors: X
authors: Li
authors: Yuzhuo
authors: Y
authors: Wang
authors: Mark
authors: M
authors: Zarnegar
authors: Chun-Yin
authors: CY
authors: Huang
authors: Jorma J
authors: JJ
authors: Palvimo
authors: Bing
authors: B
authors: Lim
authors: Zijie
authors: Z
authors: Sun
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004261:DNA Replication / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D011944:Receptors, Androgen; D025842:SUMO-1 Protein; D014157:Transcription Factors; C480599:ZMIZ1 protein, human