Neutrophil myeloperoxidase activity and the influence of two single-nucleotide promoter polymorphisms.
Myeloperoxidase (MPO) catalyses the formation of hypochlorous acid and is involved in many (patho)physiological processes. The present study was designed to determine the effect of two MPO promoter polymorphisms (463G/A and 129G/A) on enzyme activity. In 243 healthy controls, genotypes were determined and MPO activity was measured on a single-cell level using a haematological analyser. The 129G/A polymorphism reduces MPO activity in neutrophils, whereas for the 463G/A polymorphism, only gender-dependent differences in MPO activity in older age groups could be found. When studying these polymorphisms in disease, therefore, age and gender should be included in the analysis.
Rutgers
Abraham
A
Clinical and Experimental Immunology, University Hospital Maastricht, Maastricht, The Netherlands. b.rutgers@immuno.unimaas.nl
Heeringa
Peter
P
Giesen
Joyce E H M
JE
Theunissen
Ruud T
RT
Jacobs
Heinz
H
Tervaert
Jan Willem Cohen
JW
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005783:Gender Identity; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
D006997:Hypochlorous Acid; D009195:Peroxidase