Post-exposure therapy in mice against experimental rabies: a single injection of DNA vaccine is as effective as five injections of cell culture-derived vaccine.
Two rabies post-exposure therapies were comparatively evaluated: BALB/c mice were challenged at day 0 with rabies virus and then received either a single dose of rabies DNA vaccine administered at day 0, or five doses of cell culture-derived rabies vaccine administered at days 0, 3, 7, 15 and 28. Both regimens, rapidly triggered protective levels of neutralizing antibodies against rabies virus in vaccinated mice. In addition, one injection of DNA vaccine protected 53% of the challenged mice, compared to 40% of mice protected after five injections of cell culture-derived vaccine. We conclude that rabies post-exposure vaccination in BALB/c mice, based on a single administration of rabies DNA vaccine might be at least as effective as five injections of cell culture-derived vaccine.
Bahloul
Chokri
C
Laboratory of Immunology, Vaccinology and Molecular Genetics, Institut Pasteur de Tunis, 13 Place Pasteur BP-74, 1002, Tunis-Belvedere, Tunisia. chokri.bahoul@pasteur.rns.tn
Ahmed
Sami Ben Hadj
SB
B'chir
Beya Imen
BI
Kharmachi
Habib
H
Hayouni
El Akrem
el A
Dellagi
Koussay
K
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D007167:Immunotherapy; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D011818:Rabies / Q000628:therapy*; D011819:Rabies Vaccines / Q000627:therapeutic use*; D016019:Survival Analysis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000627:therapeutic use*
D000906:Antibodies; D016207:Cytokines; D011819:Rabies Vaccines; D019444:Vaccines, DNA