title: Lysis of heart endothelial cells from donor origin by cardiac graft infiltrating cells.
abstract: Endothelial cells may be involved in the acute rejection of allografts. In the present study, graft infiltrating lymphoid cell lines were propagated from a heart graft at the time of histological diagnosis of rejection. The cell lines containing CD8+ cells lysed donor-derived BLCL and endothelial cells (EC) but not third party BLCL or random EC, suggesting that HLA antigens were recognized. The cell lines containing CD4+ cells only did not lyse any target cells. The lysis of EC without preincubation with gamma interferon (gIFN) indicated that the HLA antigens recognized were class I antigens. These results suggested that lysis of donor EC may be one of the mechanisms involved in rejection.
authors: Department of Internal Medicine I, Erasmus University Rotterdam/Academic Hospital Rotterdam, Dijkzigt, Rotterdam, The Netherlands.
authors: N H
authors: NH
authors: Jutte
authors: M H
authors: MH
authors: van Batenburg
authors: C R
authors: CR
authors: Daane
authors: P
authors: P
authors: Heijse
authors: L M
authors: LM
authors: Vaessen
authors: A H
authors: AH
authors: Balk
authors: B
authors: B
authors: Mochtar
authors: F H
authors: FH
authors: Claas
authors: W
authors: W
authors: Weimar
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D004730:Endothelium, Vascular / Q000473:pathology*; D016027:Heart Transplantation / Q000276:immunology / Q000473:pathology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II