Restriction enzyme analysis of ribosomal DNA shows that Candida inconspicua clinical isolates can be misidentified as Candida norvegensis with traditional diagnostic procedures.
We identified 29 yeast isolates from 22 patients using the API ID32C panel. Twenty-eight of these isolates were Candida norvegensis and one was C. inconspicua. Although C. norvegensis is considered a pseudohypha-producing species, only one isolate produced pseudohyphae. Restriction enzyme analysis of PCR-amplified ribosomal DNA with four different enzymes proved that all isolates were C. inconspicua.
Majoros
L
L
Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. major@jaguar.dote.hu
Kardos
G
G
Belák
A
A
Maráz
A
A
Asztalos
L
L
Csánky
E
E
Barta
Z
Z
Szabó
B
B
D002175:Candida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000145:classification / Q000175:diagnosis*; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D007297:Inpatients; D010045:Outpatients; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D015183:Restriction Mapping / Q000379:methods
D004275:DNA, Ribosomal