SATB1 makes a complex with p300 and represses gp91(phox) promoter activity.
The expression of gp91(phox), the key component of the phagocyte NADPH oxidase, is regulated by various factors binding to its proximal promoter. Two nuclear matrix attachment region (MAR)-binding proteins, special AT-rich binding protein 1 (SATB1) and CCAAT displacement protein (CDP), have been reported as rare examples of gp91(phox) gene repressors. However, their individual roles and interactions with other factors in the promoter have not been elucidated in detail. We have focused on these two repressive proteins recognizing the bp -115 to bp -106 segment of the gene and obtained the following results: 1. SATB1 makes a complex, mainly with p300, regardless of the presence of DNA. 2. SATB1/p300 complex binding to the 5' upstream AT-rich region in the bp -115 to bp -106 segment represses the gp91(phox) promoter activity, and the repressed activity is partially released by CDP binding to the CCAAT element directly downstream of the AT-rich region. Our findings imply a novel role for p300 in SATB1-associated global transcription regulation.
Fujii
Yoshito
Y
Department of Host-Defense Biochemistry, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Nagasaki 852-8523, Japan. f1081@cc.nagasaki-u.ac.jp
Kumatori
Atsushi
A
Nakamura
Michio
M
D001665:Binding Sites; D002460:Cell Line; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic
D036961:Matrix Attachment Region Binding Proteins; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; C076189:SATB1 protein, human; D015534:Trans-Activators; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases