Adult T-cell leukaemia/lymphoma-like human T-cell leukaemia virus-1 replication in infective dermatitis.
Adult T-cell leukaemia/lymphoma (ATLL) is a malignant T-cell proliferation that occurs in 3-5% of individuals infected with human T-cell leukaemia virus-1 (HTLV-1). HTLV-1 infection is also linked to the development of infective dermatitis (ID), an exudative dermatitis of children that has been proposed as a cofactor of ATLL. Here, HTLV-1 replication was investigated over time in a girl with ID and multiparasitic infestation including strongyloidiasis, a disease also known to predispose HTLV-1 carriers to ATLL. Quantitative polymerase chain reaction (PCR) revealed extremely high proviral loads. During the 2-year period of the present study, the proportion of circulating infected cells ranged between 12% and 36%. Quadruplicate linker-mediated PCR amplification of HTLV-1 flanking sequences identified a pattern of extensive and persistent oligoclonal expansion of infected lymphocytes. As viral loads, both the number and the degree of infected T-cell expansion were independent of treatment or clinical signs. However, the temporal fluctuation of proviral loads correlated significantly with the degree of infected T-cell expansion, but not with the overall number of detected clones. This pattern of HTLV-1 replication over time is very different from that observed in asymptomatic carriers and reminiscent of that observed in ATLL, a result consistent with the proposal of ID as an ATLL cofactor.
Gabet
Anne-Sophie
AS
Unité d'Oncogenèse Virale, UMR5537 CNRS-Université Claude Bernard, Centre Léon Bérard, Lyon, France.
Kazanji
Mirdad
M
Couppie
Pierre
P
Clity
Emmanuel
E
Pouliquen
Jean-François
JF
Sainte-Marie
Dominique
D
Aznar
Christine
C
Wattel
Eric
E
D000914:Antibodies, Viral / Q000097:blood; D000998:Antiviral Agents / Q000627:therapeutic use; D015153:Blotting, Western / Q000379:methods; D002455:Cell Division; D002648:Child; D002999:Clone Cells; D003872:Dermatitis / Q000276:immunology* / Q000469:parasitology / Q000821:virology*; D005260:Female; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D019259:Lamivudine / Q000627:therapeutic use; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology* / Q000469:parasitology; D010272:Parasitic Diseases / Q000276:immunology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000821:virology*; D019562:Viral Load; D014779:Virus Replication
D000914:Antibodies, Viral; D000998:Antiviral Agents; D019259:Lamivudine