Development of cancer vaccines using autologous and recombinant high molecular weight stress proteins.
High molecular weight heat shock proteins (HSPs), hsp110 and grp170, derived from cancer cells have been previously shown to elicit tumor-specific immunity. This phenomenon is attributed to the antigenic peptides associated with the HSPs. Based on the unique chaperoning properties of these HSPs, a new vaccination strategy has been recently developed to elicit antigen-specific antitumor immunity. This approach utilizes tumor-associated antigens naturally complexed to these highly efficient molecular chaperones under heat shock conditions. This chapter focuses on the methodologies of these two vaccine strategies: I. purification of hsp110 and grp170 from tumor tissue or cell lines; II. generation and characterization of in vitro HSP-antigen complexes by heat shock using recombinant HSPs derived from a baculovirus protein expression system.
Wang
Xiang-Yang
XY
Department of Molecular and Cellular Biophysics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. Xiang-yang.Wang@roswellpark.org
Manjili
Masoud H
MH
Park
Juneui
J
Chen
Xing
X
Repasky
Elizabeth
E
Subjeck
John R
JR
D000818:Animals; D019496:Cancer Vaccines / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006031:Glycosylation; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000276:immunology* / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D009369:Neoplasms / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D017510:Protein Folding; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
D019496:Cancer Vaccines; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007202:Indicators and Reagents; D011994:Recombinant Proteins