Comparative map between chicken chromosome 15 and human chromosomal region 12q24 and 22q11-q12.
The physical and comparative map of GGA15 was improved by the construction of 9 BAC contigs around loci previously mapped on GGA15 by linkage analysis. In total, 240 BAC clones were isolated, covering 30-35% of GGA15, and 120 STS were developed (104 STS derived from BAC end sequences and 18 STS derived within genes). Seventeen chicken orthologues of human genes located on human Chr 22q11-q12 were directly mapped within BAC contigs of GGA15. Furthermore, the partial sequences of the chicken BAC clones were compared with sequences present in the EMBL/GenBank databases and revealed matches to 26 genes, ESTs, and genomic clones located on HSA22q11-q12 and HSA12q24. These results provide a better alignment of GGA15 with the corresponding regions in human and mouse, and improve our knowledge of the evolution and dynamics of the vertebrate genome.
Jennen
Danyel G J
DG
Wageningen Institute of Animal Sciences, Animal Breeding and Genetics Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands. danyel.jennen@wur.nl
Crooijmans
Richard P M A
RP
Kamps
Bram
B
Açar
Rukiye
R
van der Poel
Jan J
JJ
Groenen
Martien A M
MA
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D020451:Contig Mapping; D004247:DNA / Q000235:genetics; D015321:Gene Rearrangement; D005819:Genetic Markers; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D013045:Species Specificity; D038204:beta-Crystallins / Q000235:genetics
D005819:Genetic Markers; D038204:beta-Crystallins; D004247:DNA