title: Isolation of protein ligands from large peptoid libraries.
abstract: The isolation of ligands for large numbers of proteins is an important goal in proteomics. Whereas peptide libraries are rich sources of protein-binding molecules, native peptides have certain undesirable properties, such as sensitivity to proteases that make them less than ideal for some applications. We report here the construction and characterization of large, chemically diverse combinatorial libraries of peptoids (N-substituted oligoglycines). A protocol for the isolation of specific protein-binding molecules from these libraries is described. These data suggest that peptoid libraries will prove to be inexpensive and convenient sources of protein ligands.
authors: Center For Biomedical Inventions, Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8573, USA.
authors: Prasanna G
authors: PG
authors: Alluri
authors: M Muralidhar
authors: MM
authors: Reddy
authors: Kiran
authors: K
authors: Bachhawat-Sikder
authors: Hernando J
authors: HJ
authors: Olivos
authors: Thomas
authors: T
authors: Kodadek
mesh_terms: D000588:Amines / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008024:Ligands; D009687:Nuclear Proteins; D019151:Peptide Library; D034444:Peptoids / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000378:metabolism; D051736:Proto-Oncogene Proteins c-mdm2
chemical_list: D000588:Amines; D008024:Ligands; D009687:Nuclear Proteins; D019151:Peptide Library; D034444:Peptoids; D011518:Proto-Oncogene Proteins; D051736:Proto-Oncogene Proteins c-mdm2