Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato.
The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of gamma-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.
Mittova
Valentina
V
Albert Katz Department of Drylands Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Israel.
Theodoulou
Frederica L
FL
Kiddle
Guy
G
Gómez
Leonardo
L
Volokita
Micha
M
Tal
Moshe
M
Foyer
Christine H
CH
Guy
Micha
M
D000064:Acclimatization; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005721:Glutamate-Cysteine Ligase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis* / Q000737:chemistry; D019803:Glutathione Disulfide / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D018551:Solanum lycopersicum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity; D015854:Up-Regulation
D004791:Enzyme Inhibitors; D012965:Sodium Chloride; D008315:Malondialdehyde; D019328:Buthionine Sulfoximine; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D019803:Glutathione Disulfide