Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine.
In order to obtain monoclonal antibodies that might have prophylactic applications and to understand better the immune response to hepatitis E virus (HEV), we used phage display to isolate chimpanzee antibodies to HEV. The panning antigen was an two open reading frame (ORF2) recombinant protein that elicits a broadly protective immune response in vaccinated monkeys. Two major antigenic sites were identified on the ORF2 protein: one site was not accessible on the surface of infectious virions but the other site was accessible to antibodies and was recognized specifically by antibodies that neutralize the virus.
Schofield
Darren J
DJ
Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Purcell
Robert H
RH
Nguyen
Hanh T
HT
Emerson
Suzanne U
SU
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000956:Antigens, Viral / Q000187:drug effects*; D001710:Biotin; D015153:Blotting, Western; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D016752:Hepatitis E virus / Q000187:drug effects*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008253:Macaca mulatta; D002679:Pan troglodytes; D019151:Peptide Library; D011946:Receptors, Antigen / Q000187:drug effects*; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D011946:Receptors, Antigen; D014765:Viral Vaccines; D001710:Biotin