Quantitative nanopipettor for miniaturized chemical and biochemical reaction sampling.
A novel and readily available pipettor capable of nanoliter-sized volume manipulation was developed to improve and increase the flexibility of small-scale reaction processing. The volume delivery was found to be reproducible, with typical relative standard deviations of 1-5%, and easily tunable over a range of nanoliter-sized aliquots. The nanopipettor was combined with capillary electrophoresis using laser-induced fluorescence detection to monitor a small-scale enzyme reaction (beta-galactosidase) using a tetramethylrhodamine-labeled substrate. The results were in good agreement with a standard enzyme assay using a micropipet, thus demonstrating the nanopipettor's potential in developing new nanoscale utrasensitive enzyme assays.
Lorieau
Justin
J
Chemistry Department, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Shoemaker
Glen K
GK
Palcic
Monica M
MM
D003365:Costs and Cost Analysis; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D007834:Lasers; D008832:Microchemistry / Q000191:economics / Q000295:instrumentation*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000378:metabolism; D012680:Sensitivity and Specificity; D013997:Time Factors; D001616:beta-Galactosidase / Q000032:analysis* / Q000378:metabolism*
D012235:Rhodamines; C005358:tetramethylrhodamine; D001616:beta-Galactosidase