[Effects of Scatophagus argus apoptosis-related gene on apoptosis of NIH-3T3 cells].
OBJECTIVE
To observe the effects of Scatophagus argus apoptosis-related (SaAR) gene transfer on the apoptosis of NIH-3T3 cells.


METHODS
A controlled observation of SaAR gene transfer (at different concentrations) into cultured NIH-3T3 cells via lipofectin was performed, and the rate of cell apoptosis was analyzed by flow cytometry.


RESULTS
The rate of cell apoptosis was (6.37+/-0.0.56)% in the control group, and was (11.89+/-1.13) %, (18.25+/-1.86) % and (22.34+/-1.08) % respectively in pcDNA3-SaAR gene groups corresponding to pcDNA3-SaAR gene concentrations of 2, 3 and 4 microg/ml respectively. It was shown that SaAR gene significantly induced fibroblast apoptosis when the concentration was above 2 microg/ml (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05).


CONCLUSIONS
Transient transfection of SaAR gene can significantly induce apoptosis in NIH-3T3 cells.
Ouyang
Ping
P
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. ypou@fimmu.com
Chen
Hui-ping
HP
Lai
Wen-yan
WY
Yang
Wen-li
WL
Xu
An-long
AL
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005399:Fishes; D022761:Gene Transfer, Horizontal; D051379:Mice; D041681:NIH 3T3 Cells
D018076:DNA, Complementary