Production and characterization of alpha-galactosidase from Aspergillus flavipes.
An extracellular alpha-galactosidase from the culture filtrate of Aspergillus flavipes grown on melibiose as a carbon source was partially purified by hydroxylapatite and diethylaminoethylcellulose chromatographies. Electrophoretic analysis showed protein bands corresponding to alpha-galactosidase and invertase activities. The optimum pH and temperature were determined as 4.5-5.0 and 45 degrees C, respectively. The Km value for p-nitrophenyl-alpha-d-galactopyranoside was found to be 1.89 mm. The results reported in this study indicate that Aspergillus flavipes is indeed an active source of extracellular alpha-galactosidase.
Ozsoy
Nurten
N
Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34452 Beyazit, Istanbul, Turkey.
Berkkan
Hakan
H
D001230:Aspergillus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D000519:alpha-Galactosidase / Q000302:isolation & purification* / Q000378:metabolism*
D000519:alpha-Galactosidase