Endothelin-1 decreases ethanolamine plasmalogen levels and evokes PAF production in brain microvessels.
Treatment of brain microvessels with Endothelin-1 evoked a decrease in ethanolamine plasmalogen levels by calcium-independent phospholipase A(2). In contrast, the diacyl molecular forms of ethanolamine phospholipids were unaffected. Evidence also shows that Endothelin type A receptors are involved. Concomitantly, PAF production mediated by CoA-independent transacylase was observed. This is the first evidence of involvement of these pathways on the Endothelin-1 mechanism of action on the blood-brain barrier.
Collado
M Pilar
MP
Departamento de Bioquímica y Biología Molecular I, Facultad de Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
Latorre
Eduardo
E
Fernández
Inmaculada
I
Aragonés
M Dolores
MD
Catalán
R Edgardo
RE
D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000098:blood supply* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000502:physiology*; D008833:Microcirculation; D010741:Phospholipases A / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010955:Plasmalogens / Q000096:biosynthesis*; D010972:Platelet Activating Factor / Q000378:metabolism*; D044022:Receptor, Endothelin A / Q000378:metabolism
D019332:Endothelin-1; D010743:Phospholipids; D010955:Plasmalogens; D010972:Platelet Activating Factor; D044022:Receptor, Endothelin A; C020791:phosphatidal ethanolamines; D010741:Phospholipases A; D002118:Calcium