Separation of cefoperazone enantiomers using beta-cyclodextrin as chiral additive by capillary zone electrophoresis.
A capillary electrophoresis method was developed to separate the enantiomers of cefoperazone. Different cyclodextrins, including alpha-cyclodextrin (alpha-CD), beta-cyclodextrin (beta-CD), gamma-cyclodextrin (gamma-CD), 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD), and methyl-beta-cyclodextrin (Me-beta-CD), were tested as chiral additives in the running buffer. The effect of various parameters on enantioseparation such as concentration of NaH(2)PO(4), buffer pH, and CD concentration was also studied. The cefoperazone enantiomers were baseline separated under conditions of 0.04 mmol/L beta-CD, 75 mmol/L NaH(2)PO(4) buffer at pH 4.0. A fused silica capillary (40 cm effective length x 75 microm ID) was used. The applied voltage and capillary temperature were 20 kV and 25 degrees C, respectively. Under these conditions, linear calibration curves were obtained in the 5-500 microg/ml range using UV detection at 280 nm. The limit of detection for both isomers was 0.1 microg/ml. The method was used for the analysis of different pharmaceutical preparations (dose) and biological samples containing cefoperazone.
Wang
Rong
R
Department of Pharmacy, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou, China.
Jia
Zheng-Ping
ZP
Fan
Jun-Jie
JJ
Hu
Xiao-Li
XL
Li
Yong-Min
YM
Chen
Li-Ren
LR
Xie
Jing-Wen
JW
Zhang
Qiang
Q
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000652:urine; D002021:Buffers; D002438:Cefoperazone / Q000737:chemistry* / Q000302:isolation & purification* / Q000652:urine; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010756:Phosphoric Acids; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D000900:Anti-Bacterial Agents; D002021:Buffers; D003505:Cyclodextrins; D010756:Phosphoric Acids; D047392:beta-Cyclodextrins; D002438:Cefoperazone; C030242:phosphoric acid; C031215:betadex