title: [Influence of KATP channel inhibitor on the changes of HSP70 expression in sevoflurane-induced neonatal rat cardiomyocytes].
abstract: OBJECTIVE
  To study the roles of KATP channel and HSP70 in sevoflurane-induced preconditioning in neonatal rat cardiomyocytes and their mutual relationship.
  
  
  METHODS
  The second generation of primary cultured cardiomyocytes were randomly divided into 5 groups; normal control, anoxia/reoxygenation, sevoflurane preconditioning, glyburide and glyburide plus sevoflurane. In each group, the cardiomyocytes were exposed to a 2-hour anoxia, followed by a 48-hour reoxygenation. We detected HSP70 expression at 0, 1, 12, 24, 36 and 48 hours after reoxygenation respectively.
  
  
  RESULTS
  At each time-point of reoxygenation, the expression of HSP70 in sevoflurane preconditioning group was significantly higher than that of normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups (P < 0.01). There was no significant difference concerning HSP70 expression among normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups (P > 0.05).
  
  
  CONCLUSIONS
  Both HSP70 and KATP channel may be involved in the process of sevoflurane preconditioning in neonatal rat cardiomyocytes. Blocking the KATP channel can inhibit the expression of HSP70.
authors: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors: Ying
authors: Y
authors: Tang
authors: Quanyun
authors: Q
authors: Wang
authors: Jingyi
authors: J
authors: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005905:Glyburide / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D019157:Ischemic Preconditioning, Myocardial; D008738:Methyl Ethers / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008738:Methyl Ethers; D026902:Potassium Channel Blockers; D000077149:Sevoflurane; D000255:Adenosine Triphosphate; D005905:Glyburide