title: CbiX-homologous protein (CbiXhp), a metal-binding protein, from Streptomyces seoulensis is involved in expression of nickel-containing superoxide dismutase.
abstract: To find the accessory proteins participating in expression and maturation of nickel-containing superoxide dismutase (NiSOD), a metal-binding protein (CbiXhp) homologous to cobaltochelatase (CbiX) of Bacillus megaterium was isolated by nickel-nitrilotriacetic acid resin from Streptomyces seoulensis. The deduced amino acid sequence of cbiXhp showed 87% and 39% identity to CbiX of Streptomyces coelicolor and that of B. megaterium, respectively. Overexpression of CbiXhp increased the activity and the expression of NiSOD in the presence and absence of nickel, but to a lesser extent in its absence. This result indicates that CbiXhp is involved in the expression of NiSOD.
authors: Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea.
authors: In-Kwon
authors: IK
authors: Kim
authors: Yang-In
authors: YI
authors: Yim
authors: Young-Min
authors: YM
authors: Kim
authors: Jin-Won
authors: JW
authors: Lee
authors: Hyung-Soon
authors: HS
authors: Yim
authors: Sa-Ouk
authors: SO
authors: Kang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008190:Lyases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism*; D011485:Protein Binding; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009532:Nickel; D013482:Superoxide Dismutase; D008190:Lyases; C033211:cobaltochelatase