The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea.
Brassinosteroids (BRs) are growth-promoting plant steroid hormones, and in garden pea (Pisum sativum L.), the lka mutant is defective in BR perception. Here, we show that LKA encodes P. sativum BRI1 (PsBRI1), a homolog of BRI1, which is the Arabidopsis leucine-rich repeat receptor-like kinase/BR receptor. PsBRI1 was isolated by screening a pea cDNA library using Arabidopsis BRI1 cDNA as the probe. PsBRI1 is predicted to encode a 1188-amino-acid protein that has 78% similarity with Arabidopsis BRI1. Sequence analysis of PsBRI1 in the lka mutant led to the identification of a missense mutation that converts the highly conserved aspartic acid residue to asparagine, which is located in the leucine-rich repeat, just before the island domain that may bind BR or a BR-protein complex. The mutation identified in PsBRI1 co-segregated with the semi-erectoide lka phenotype. Transcript analysis of LKA/PsBRI1 indicates that it is ubiquitously expressed in pea and that the expression was downregulated by exogenous BR. The lka mutant was then utilized in further studies to analyze the independent actions of BR and gibberellin (GA) through the characterization of BR response on GA mutants and GA response on BR mutants.
Nomura
Takahito
T
Department of Biosciences, Teikyo University, Utsunomiya 320-8551, Japan.
Bishop
Gerard J
GJ
Kaneta
Tsuyoshi
T
Reid
James B
JB
Chory
Joanne
J
Yokota
Takao
T
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D015723:Gene Library; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D020449:Repetitive Sequences, Amino Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics
D029681:Arabidopsis Proteins; D017931:DNA Primers; D011494:Protein Kinases