Assessment of laboratory performance of nucleic acid amplification tests for detection of Mycobacterium tuberculosis.
During implementation of the Centers for Disease Control and Prevention's Mycobacterium tuberculosis nucleic acid amplification (NAA) evaluation program, 27.1% of participants used the same biological safety cabinet for NAA and specimen processing; 28.8% reported not using unidirectional workflow. An association between false positives and adverse responses to quality assurance questions (P = 0.04) illustrated the need for following NCCLS recommendations.
Ridderhof
John C
JC
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Williams
Laurina O
LO
Legois
Sue
S
Shult
Peter A
PA
Metchock
Beverly
B
Kubista
Louise N
LN
Handsfield
James H
JH
Fehd
Ronald J
RJ
Robinson
Pamela H
PH
D002487:Centers for Disease Control and Prevention, U.S.; D005784:Gene Amplification; D005821:Genetic Techniques; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012449:Safety / Q000592:standards; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D014481:United States