title: Comparison of PCR-enzyme-linked immunosorbent assay and real-time PCR assay for diagnosis of an unusual case of cerebral toxoplasmosis in a stem cell transplant recipient.
abstract: A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.
authors: Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Paris, France. jean.menotti@sls.ap-hop-paris.fr
authors: Jean
authors: J
authors: Menotti
authors: Gustavo
authors: G
authors: Vilela
authors: Stéphane
authors: S
authors: Romand
authors: Yves Jean-François
authors: YJ
authors: Garin
authors: Lionel
authors: L
authors: Ades
authors: Eliane
authors: E
authors: Gluckman
authors: Francis
authors: F
authors: Derouin
authors: Patricia
authors: P
authors: Ribaud
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014122:Toxoplasma / Q000145:classification / Q000302:isolation & purification*; D016781:Toxoplasmosis, Cerebral / Q000097:blood / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G