High expression of YB-1 gene in erythroid cells in patients with refractory anemia.
It has been shown that aberrant expression of a transcription factor, GATA-1, leads to maturation arrest and transformation of erythroid cells. We previously reported that a multifunctional protein, YB-1, was expressed strongly in the spleen of a GATA-1 mutant mouse, which was filled with transformed erythroblasts. This finding suggested that YB-1 has roles in erythropoiesis. In this study, we examined in vivo expression of YB-1 messenger RNA (mRNA) in bone marrow erythroid cells and erythroid leukemic cell lines. During erythroid differentiation of erythroid leukemic cell lines, the expression level of YB-1 mRNA was highest at the early phase of differentiation and then decreased. In human bone marrow cells, the in vivo expression level of YB-1 mRNA was higher in glycophorin A-positive cells than in glycophorin A-negative cells. An interesting finding was that expression of YB-1 was higher in erythroblasts in myelodysplastic syndrome-refractory anemia (MDS-RA) than in normal cells. The findings suggested that YB-1 functions in the early stage of erythropoiesis and that aberrant expression of this protein may induce hematological diseases such as MDS.
Yokoyama
Hisayuki
H
Department of Rheumatology and Hematology, Tohoku University School of Medicine, Sendai, Japan.
Harigae
Hideo
H
Takahashi
Shinichiro
S
Kameoka
Junichi
J
Miyamura
Koichi
K
Ishizawa
Kenichi
K
Kaku
Mitsuo
M
Sasaki
Takeshi
T
D000753:Anemia, Refractory / Q000097:blood*; D001854:Bone Marrow Cells; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins; D004912:Erythrocytes / Q000378:metabolism*; D004920:Erythropoiesis / Q000235:genetics; D006021:Glycophorins / Q000032:analysis; D006801:Humans; D020014:K562 Cells; D009190:Myelodysplastic Syndromes / Q000209:etiology; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D006021:Glycophorins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human