title: Identification and metabolic role of the mitochondrial aspartate-glutamate transporter in Saccharomyces cerevisiae.
abstract: The malate-aspartate NADH shuttle in mammalian cells requires the activity of the mitochondrial aspartate-glutamate carrier (AGC). Recently, we identified in man two AGC isoforms, aralar1 and citrin, which are regulated by calcium on the external face of the inner mitochondrial membrane. We have now identified Agc1p as the yeast counterpart of the human AGC. The corresponding gene was overexpressed in bacteria and yeast mitochondria, and the protein was reconstituted in liposomes where it was identified as an aspartate-glutamate transporter from its transport properties. Furthermore, yeast cells lacking Agc1p were unable to grow on acetate and oleic acid, and had reduced levels of valine, ornithine and citrulline; in contrast they grew on ethanol. Expression of the human AGC isoforms can replace the function of Agc1p. However, unlike its human orthologues, yeast Agc1p catalyses both aspartate-glutamate exchange and substrate uniport activities. We conclude that Agc1p performs two metabolic roles in Saccharomyces cerevisiae. On the one hand, it functions as a uniporter to supply the mitochondria with glutamate for nitrogen metabolism and ornithine synthesis. On the other, the Agc1p, as an aspartate-glutamate exchanger, plays a role within the malate-aspartate NADH shuttle which is critical for the growth of yeast on acetate and fatty acids as carbon sources. These results provide strong evidence of the existence of a malate-aspartate NADH shuttle in yeast.
authors: Departmento Biología Molecular, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-C S I C, Madrid, Spain.
authors: S
authors: S
authors: Cavero
authors: A
authors: A
authors: Vozza
authors: A
authors: A
authors: del Arco
authors: L
authors: L
authors: Palmieri
authors: A
authors: A
authors: Villa
authors: E
authors: E
authors: Blanco
authors: M J
authors: MJ
authors: Runswick
authors: J E
authors: JE
authors: Walker
authors: S
authors: S
authors: Cerdán
authors: F
authors: F
authors: Palmieri
authors: J
authors: J
authors: Satrústegui
mesh_terms: D000085:Acetates / Q000378:metabolism; D026922:Amino Acid Transport Systems, Acidic / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism*; D009952:Ornithine / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D026922:Amino Acid Transport Systems, Acidic; D017920:Antiporters; D008081:Liposomes; D008293:Malates; D020033:Protein Isoforms; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C051046:aspartate-glutamate carrier; D002245:Carbon Dioxide; D001224:Aspartic Acid; D018698:Glutamic Acid; C030298:malic acid; D009952:Ornithine