Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR.
We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of bacterial isolates via computer software.
Meacham
Kiersten J
KJ
Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA.
Zhang
Lixin
L
Foxman
Betsy
B
Bauer
Richard J
RJ
Marrs
Carl F
CF
D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005060:Europe; D005843:Geography; D006801:Humans; D007438:Introns; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results
D017931:DNA Primers