3M Petrifilm Staph Express Count plate method for the enumeration of Staphylococcus aureus in selected types of meat, seafood, and poultry: collaborative study.
The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method 975.55 for the enumeration of Staphylococcus aureus in selected foods. Four foods--cooked, diced chicken; cured ham; smoked salmon; and pepperoni--were analyzed for S. aureus by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample, a low inoculation level, a medium inoculation level, and a medium inoculation level with background flora, each in duplicate. The mean log10 counts for the methods were comparable for all 4 foods. The repeatability and reproducibility variances of the 24 h Petrifilm Staph Express Count plate method were similar to those of the 72 h standard method.
McMahon
Wendy A
WA
Silliker, Inc., Research Center, 160 Armory Dr, South Holland, IL 60473, USA. wendy.mcmahon@silliker.com
Aleo
Victoria A
VA
Schultz
Ann M
AM
Horter
Barbara L
BL
Lindberg
Kathryn G
KG
D000818:Animals; D002645:Chickens; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D005511:Food Handling; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology; D011200:Poultry / Q000382:microbiology*; D015203:Reproducibility of Results; D012474:Salmon / Q000382:microbiology; D017747:Seafood / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013552:Swine