title: [Impression cytology study of dry eyes in patients with congenital aniridia].
abstract: OBJECTIVE
  To evaluate the long-term results of ocular surface reconstruction by means of medical treatment, limbal transplantation or amniotic membrane transplantation in patients with congenital aniridia.
  
  
  METHODS
  Thirty-six eyes from 18 consecutive patients with aniridia were studied by clinical tests (Schirmer test, break-up time and vital rose bengal staining) and impression cytology. Thirteen patients were treated with lubricant drops for ocular dryness. Two eyes from 2 patients also received limbal transplantation, and other 4 eyes underwent amniotic membrane transplantation.
  
  
  RESULTS
  Corneal and conjunctival epithelium improved in all patients treated with lubricant drops. Ocular surface epithelium condition was worse in those patients not receiving artificial tears. After 12 months of limbal transplantation, clinical and morphological results showed a significant corneal recovery of at least two grades of squamous metaplasia in comparison with the previous situation. We also carried out amniotic membrane transplantation in patients with aniridia. The improvement was initially observed after 2 months and by the sixth month there was a significant improvement in clinical signs and symptoms, with normal corneal cells next to altered ones.
  
  
  CONCLUSIONS
  All the aniridic patients showed dry eye. Treatment with lubricant drops is necessary to decrease the squamous metaplasia process. Limbal transplantation and amniotic membrane implantation are needed for effective corneal surface reconstruction in patients with aniridia, which is followed by a significant improvement of the dry eye.
authors.affiliation: Servicio de Oftalmología, Hospital Universitario Ramón y Cajal, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Rivas
authors.forename: J
authors.initials: J
authors.lastname: Murube
authors.forename: A
authors.initials: A
authors.lastname: Rivas
authors.forename: E
authors.initials: E
authors.lastname: Murube
mesh_terms: D000293:Adolescent; D000328:Adult; D015783:Aniridia / Q000150:complications / Q000473:pathology*; D015352:Dry Eye Syndromes / Q000150:complications / Q000473:pathology* / Q000628:therapy*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged