Automated multi-dimensional purification of tagged proteins.
The capacity for high throughput purification (HTP) is essential in fields such as structural genomics where large numbers of protein samples are routinely characterized in, for example, studies of structural determination, functionality and drug development. Proteins required for such analysis must be pure and homogenous and available in relatively large amounts. AKTA 3D system is a powerful automated protein purification system, which minimizes preparation, run-time and repetitive manual tasks. It has the capacity to purify up to 6 different His6- or GST-tagged proteins per day and can produce 1-50 mg protein per run at >90% purity. The success of automated protein purification increases with careful experimental planning. Protocol, columns and buffers need to be chosen with the final application area for the purified protein in mind.
Sigrell
Jill A
JA
R&D Separations, Amersham Biosciences AB, Björkgatan 30, 751 84 Uppsala, Sweden. jill.sigrell@amersham.com
Eklund
Pär
P
Galin
Markus
M
Hedkvist
Lotta
L
Liljedahl
Pia
P
Johansson
Christine Markeland
CM
Pless
Thomas
T
Torstenson
Karin
K
D001331:Automation; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics; D021621:Imaging, Three-Dimensional; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012984:Software
D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D006639:Histidine; D005982:Glutathione Transferase