Oestrogen receptor-beta and neurohypophysial hormones: functional interaction and neuroanatomical localisation.
Oestrogens affect fluid balance, influencing both ingestive behaviour and renal excretion. The renal effects are partly due to altered release of vasopressin and oxytocin. This study was designed to explore the role of oestrogen receptor-beta (ERbeta) in neurohypophysial hormonal function. Following dietary administration, soya isoflavones reach the brain in sufficient concentration to activate ERbeta, but not oestrogen receptor-alpha (ERalpha). ERbeta function was therefore manipulated by feeding rat diets differing in soya isoflavone content. Fluid balance and neurohypophysial hormone release were measured in male rats maintained for 14 days on a soya isoflavone-free diet or one containing 150 microg/g genistein+daidzein. Food and water intake, body weight, urine flow, osmolality and sodium concentrations were determined daily. After 14 days, plasma and urine osmolality and sodium, vasopressin and oxytocin concentrations were determined. There was no significant difference in weight gain between the two groups or in their excretion of sodium and water or plasma sodium and plasma oxytocin. However, plasma vasopressin was significantly lower in the iso-free group. Double-label immunocytochemistry was used to assess colocalisation of ERbeta with the neurohypophysial hormones in male rats. Cell nuclei showing ERbeta immunoreactivity were abundant in the posterior magnocellular paraventricular nucleus (PVNpm) and in the supraoptic nucleus (SON). Vasopressin-immunoreactive neurones were similarly distributed, forming the core of the PVNpm and the ventral portion of the SON; majority were positive for ERbeta. Cells with oxytocin immunoreactivity were located mainly at the periphery of the PVNpm and in the dorsal SON; only approximately a quarter of these cells showed ERbeta immunoreactivity. Thus, the difference in the effects of the soya diet on vasopressin and oxytocin release may be related to the ERbeta-activating properties of this diet and to the preponderance of this receptor in vasopressin as opposed to oxytocin cells.
Forsling
M L
ML
Neuroendocrine and Psychopharmacology Research Laboratories, Centre for Neuroscience, King's College London, Guy's Campus, London SE1 1UL, UK. mary.forsling@kcl.ac.uk
Kalló
I
I
Hartley
D E
DE
Heinze
L
L
Ladek
R
R
Coen
C W
CW
File
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D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D047629:Estrogen Receptor beta; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009994:Osmolar Concentration; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000033:anatomy & histology* / Q000187:drug effects; D010909:Pituitary Hormones, Posterior / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects*; D013025:Glycine max; D013495:Supraoptic Nucleus / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000187:drug effects
D047629:Estrogen Receptor beta; D010909:Pituitary Hormones, Posterior; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014667:Vasopressins; D010121:Oxytocin