Characterization of porphyrins of rat Harderian gland by thin layer chromatography and mass spectrometry: no evidence for a tricarboxylic acid porphyrin.
Reversed-phase TLC systems using ion-pairing reagents are described for the separation without prior derivatization of the porphyrins of rat Harderian gland. Porphyrins with R(f) values greater than protoporphyrin but smaller than coproporphyrin, representing harderoporphyrin, were labile to 1 m NaOH, which converted them to protoporphyrin, inconsistent with the established literature that harderoporphyrin is a tricarboxylic acid porphyrin. It was confirmed by HPLC/electrospray ionization MS that this 'harderoporphyrin' fraction consists of the recently characterized glycoconjugate, protoporphyrin-1-O-acyl beta-xyloside, with trace amounts of protoporphyrin-1-O-acyl beta-glucoside.
Gorchein
Abel
A
MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck College, Malet Street, London WC1E 7HX, UK. a.gorchein@sbc.bbk.ac.uk
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D006243:Harderian Gland / Q000737:chemistry*; D008297:Male; D011166:Porphyrins / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014233:Tricarboxylic Acids / Q000737:chemistry*
D011166:Porphyrins; D014233:Tricarboxylic Acids