Mitochondrial cytochrome b variation in populations of the visceral leishmaniasis vector Lutzomyia longipalpis across eastern Brazil.
A population analysis of peridomestic, light-trapped, field specimens of the phlebotomine sand fly Lutzomyia longipalpis was targeted to six locations representing a geographic transect across eastern Brazil. Mitochondrial cytochrome b gene sequences established the pattern of genetic variation among the populations. Alignment of a 261-basepair region at the 3' end of cytochrome b identified 30 haplotypes and 21 segregating sites from 78 sand flies. Pairwise comparisons indicated statistically significant population structuring between northern and southern populations, as well as structuring among the southern populations. Prominent spatial clustering was evident for two of the populations in a minimum spanning network of the haplotypes, but sequence divergence was not sufficient to indicate cryptic species.
Hodgkinson
Virginia H
VH
Biology Department, Fairfield University, Fairfield, Connecticut 06824, USA. vhodgkinson@mail.fairfield.edu
Birungi
Josephine
J
Quintana
Miguel
M
Dietze
Reynaldo
R
Munstermann
Leonard E
LE
Deitze
Reynaldo
R
D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis*; D014644:Genetic Variation; D005843:Geography; D006801:Humans; D007303:Insect Vectors / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000635:transmission*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011156:Population Density; D011576:Psychodidae / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b