title: Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes).
abstract: Ultra violet (UV) screens and preservatives are widely and increasingly used in cosmetics and pharmaceuticals. In the present study, we examined the estrogenicity of 4-methyl-benzylidene camphor (4-MBC), octyl-methoxycinnamate (OMC), and propyl paraben (n-propyl-p-hydroxy-benzoate; PP), among UV screens and preservatives, using male medaka (Oryzias latipes), in regard to production of vitellogenin (VTG) and choriogenin (CHG) which are known to be estrogen-responsive gene products. First, using a VTG enzyme-linked immunosorbent assay (ELISA) system, we determined the increase in VTG plasma concentration in medaka due to exposure to 4-MBC, OMC, and PP, and compared this concentration to the non-treated control. Next, we found increases in mRNA expression levels of VTG subtypes VTG-1 and VTG-2, and CHG subtypes CHG-L and CHG-H, in liver due to exposure to 4-MBC, OMC, and PP compared to the non-treated control. In addition, we also found increased mRNA expression levels of estrogen receptor (ER) alpha, among sex hormone receptors in the liver, due to exposure to 4-MBC, OMC, and PP compared to the non-treated control. In this study, we showed that 4-MBC, OMC, and PP have estrogenic activity in fish.
authors.affiliation: Department of Applied Biological Chemistry, Osaka Prefecture University, Osaka 599-8531, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Inui
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Adachi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takenaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inui
authors.forename: Masami
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ueda
authors.forename: Hajime
authors.initials: H
authors.lastname: Watanabe
authors.forename: Chisato
authors.initials: C
authors.lastname: Mori
authors.forename: Taisen
authors.initials: T
authors.lastname: Iguchi
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Miyatake
mesh_terms: D000818:Animals; D002164:Camphor / Q000031:analogs & derivatives* / Q000633:toxicity; D002934:Cinnamates / Q000633:toxicity; D004527:Egg Proteins / Q000096:biosynthesis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha; D029941:Fish Proteins / Q000096:biosynthesis* / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009990:Oryzias / Q000097:blood / Q000378:metabolism*; D010226:Parabens / Q000633:toxicity; D011498:Protein Precursors / Q000096:biosynthesis* / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013473:Sunscreening Agents / Q000633:toxicity*; D014819:Vitellogenins / Q000096:biosynthesis* / Q000097:blood
chemical_list: D002934:Cinnamates; D004527:Egg Proteins; D047628:Estrogen Receptor alpha; D029941:Fish Proteins; C092024:L-SF protein, Oryzias latipes; D010226:Parabens; D011498:Protein Precursors; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D013473:Sunscreening Agents; D014819:Vitellogenins; C104950:choriogenin H; C118580:octylmethoxycinnamate; D002164:Camphor; C038939:enzacamene; C006068:propylparaben