title: Identification using LC-MSn of co-metabolites in the biosynthesis of the polyketide toxin mycolactone by a clinical isolate of Mycobacterium ulcerans.
abstract: LC-MSn analysis of mycolactone toxin from extracts of Mycobacterium ulcerans has shown that minor co-metabolites, including two previously unreported, differ structurally from mycolactone only in a small portion of the polyketide side-chain.
authors.affiliation: Department of Chemistry and Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Hui
authors.initials: H
authors.lastname: Hong
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Gates
authors.forename: James
authors.initials: J
authors.lastname: Staunton
authors.forename: Tim
authors.initials: T
authors.lastname: Stinear
authors.forename: Stewart T
authors.initials: ST
authors.lastname: Cole
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Spencer
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D018942:Macrolides; D015394:Molecular Structure; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D019911:Mycobacterium ulcerans / Q000302:isolation & purification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001427:Bacterial Toxins; D018942:Macrolides; C117218:mycolactone