title: [Glutamate-neuroexcitotoxicity protective study of monoclonal antibody against human NMDA receptor key subunit].
abstract: OBJECTIVE
  To study if monoclonal antibody against human NMDA receptor key subunit (NR1) may protect neurons from excitotoxicity.
  
  
  METHODS
  We cultured primary hippocampal neurons from 10 newborn rats and made the glutamate excitotoxicity model, examined the ratio of surviving neuron (Trypan blue dye staining) and LDH assay to study the protective effects of mAbN1, There were 2 plates in parallel per group, the experiments were repeated 4 times.
  
  
  RESULTS
  0.3 micro mol/L mAbN1 could protect cultured hippocampal neurons from neuroexcitotoxicity induced by 500 micro mol/L glutamate with increased survival rate 35%. Epitope peptide could block the protection of mAbN1, which suggests that the protection of mAbN1 is specific.
  
  
  CONCLUSIONS
  mAbN1 is a novel NMDAR blocker with in vitro neuroprotective activity which may provide a basis for antibody therapy of neuroexcitotoxicity.
authors.affiliation: Institute for Brain Disorders, Dalian Medical University, Dalian 116027, China.
authors.forename: Xiao-nan
authors.initials: XN
authors.lastname: Kang
authors.forename: Chang-kai
authors.initials: CK
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Ai-shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Ji-qQing
authors.initials: JQ
authors.lastname: Wang
authors.forename: Da-yue
authors.initials: DY
authors.lastname: Han
authors.forename: Guang-xia
authors.initials: GX
authors.lastname: Shi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000633:toxicity*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D021122:Protein Subunits; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*
chemical_list: D000911:Antibodies, Monoclonal; D018696:Neuroprotective Agents; D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid