Protease-activated receptor isoform expression in pregnant and nonpregnant rat myometrial tissue.
OBJECTIVE
Three protease-activated receptor (PAR1, 3, and 4) isoforms have been shown to be responsible for the cellular effects of thrombin; another PAR isoform (PAR2) is responsible for the cellular effects of trypsin. The present studies sought to test the hypothesis that one (or more) of these PAR isoforms is expressed in myometrial tissue, thereby accounting for the uterotonic effects of these novel agonists.


METHODS
The rat PAR3 and 4 isoforms were cloned from a rat spleen cDNA library. PAR isoform mRNA expression was determined by using reverse-transcriptase polymerase chain reactions (PCR) in Sprague-Dawley rats. Confirmation of the identity of the amplified mRNA was done by sequence analysis. Relative quantification of the PAR1 and PAR2 isoforms was performed using a real-time quantitative reverse transcriptase PCR (RT-PCR) technique. PAR protein expression was confirmed by Western blots using polyclonal antibodies.


RESULTS
The rat PAR3 and 4 homologues showed significant sequence homology to the mouse and human amino acid and nucleotide sequences. The RT-PCR studies confirmed PAR1-4 expression in myometrium from rats in estrus. PAR3 was expressed in uterus, spleen, kidney, liver, lung, brain, and heart. PAR4 was expressed in uterus, spleen, and lung. Messenger RNA for the PAR1 and 2 isoforms was expressed during the second half of gestation in myometrium from timed-pregnant rats. In contrast, mRNA for the PAR3 and 4 isoforms was not detected in gestational myometrium. PAR protein expression appeared to match tissue mRNA expression patterns.


CONCLUSIONS
These RT-PCR studies confirmed ubiquitous expression of the PAR1 and PAR2 isoforms in myometrium and other rat tissues; in contrast, the PAR3 and PAR4 isoforms are expressed in a tissue-specific and gestationally related pattern.
Chien
Edward K
EK
Department of Obstetrics and Gynecology, University of Vermont, Given Building C258, 89 Beaumonmt Drive, Burlington, VT 05405, USA. Edward.Chien@uvm.edu
Sweet
Leigh
L
Phillippe
Mark
M
Marietti
Sarah
S
Kim
Terrence T
TT
Wolff
David A
DA
Thomas
Leandra
L
Bieber
Eric
E
D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D004971:Estrus / Q000502:physiology; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D009215:Myometrium / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D044463:Receptor, PAR-1 / Q000235:genetics / Q000378:metabolism*; D044464:Receptor, PAR-2 / Q000235:genetics / Q000378:metabolism; D044462:Receptors, Proteinase-Activated / Q000235:genetics / Q000378:metabolism*
D020033:Protein Isoforms; D044463:Receptor, PAR-1; D044464:Receptor, PAR-2; D044462:Receptors, Proteinase-Activated; C481941:protease-activated receptor 3, mouse; C481942:protease-activated receptor 4, mouse