title: Differential release of alpha-melanophore stimulating hormone isoforms by the pituitary gland of red porgy, Pagrus pagrus.
abstract: The best known actions of the pleiotrope alpha-melanophore stimulating hormone (alpha-MSH) are skin pigment regulation and corticotrope actions in the response to chronic stress. Stress-induced and enhanced release of alpha-MSH may therefore influence skin pigmentation and stress physiology simultaneously. The release of alpha-MSH is under multiple control by hypothalamic hormones and neurotransmitters. Thyrotropin releasing hormone (TRH), corticotropin releasing hormone (CRH), melanophore concentrating hormone (MCH), and dopamine (DA) have been tested in a superfusion set up for their potential to regulate alpha-MSH release from the pituitary gland of red porgy, Pagrus pagrus, in vitro. The release of alpha-MSH was stimulated by TRH and CRH, and was inhibited by MCH and DA. During unstimulated (basal) release, mono-acetylated alpha-MSH was the dominant form. During superfusion with secretagogues, we found that independent of their inhibitory or stimulatory capacity, isoform frequency did not change. MSH-isoform ratios were similar for all the substances that were used, except that both the inhibitory and the stimulatory factors increased the percentage of di-acetylated alpha-MSH at low concentrations (10(-11)M) when compared to their effects at high concentrations (10(-7)M).
authors.affiliation: Department of Organismal Animal Physiology, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands. angesalm@sci.kum.nl
authors.forename: A L
authors.initials: AL
authors.lastname: Van der Salm
authors.forename: M
authors.initials: M
authors.lastname: Pavlidis
authors.forename: G
authors.initials: G
authors.lastname: Flik
authors.forename: S E
authors.initials: SE
authors.lastname: Wendelaar Bonga
mesh_terms: D000107:Acetylation; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D007028:Hypothalamic Hormones / Q000494:pharmacology; D008543:Melanins / Q000494:pharmacology; D010473:Perciformes; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D010907:Pituitary Hormones / Q000494:pharmacology; D020033:Protein Isoforms / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D000521:alpha-MSH / Q000378:metabolism*
chemical_list: D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D020033:Protein Isoforms; D000521:alpha-MSH; D013973:Thyrotropin-Releasing Hormone; C019463:melanin-concentrating hormone; D003346:Corticotropin-Releasing Hormone; D004298:Dopamine