title: Crystallization of RusA Holliday junction resolvase from Escherichia coli.
abstract: Crystals of the Escherichia coli Holliday junction resolvase RusA have been obtained using the hanging-drop method and characterized. The crystals have a primitive monoclinic form and belong to space group P2(1). The V(M) value suggests the presence of two copies of the monomer in the asymmetric unit. A full three-wavelength MAD data collection on a selenomethionine-incorporated form has been undertaken and structure determination is under way using data collected to 2.1 A resolution.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, England.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Muranova
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Sedelnikova
authors.forename: Philip M
authors.initials: PM
authors.lastname: Leonard
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pasquo
authors.forename: Edward L
authors.initials: EL
authors.lastname: Bolt
authors.forename: Robert G
authors.initials: RG
authors.lastname: Lloyd
authors.forename: John B
authors.initials: JB
authors.lastname: Rafferty
mesh_terms: D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D045565:Holliday Junction Resolvases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D012645:Selenomethionine; D045565:Holliday Junction Resolvases; C112057:RusA protein, E coli