title: Isolation of Burkholderia cepacia complex genomovars from waters.
abstract: The aim of this study was to develop a selective enrichment broth as an aid for the isolation of Burkholderia cepacia complex (Bcc) bacteria from water. To allow growth of all nine genomovars, mixtures of two carbon sources had to be used, i.e. L-arabinose/D-cellobiose or L-arabinose/L-threonine. Selectivity was provided by polymyxin B and 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390). Following enrichment, Bcc bacteria were isolated on a diagnostic O/F agar supplemented with gentamicin. A preliminary bio-diversity study on 28 surface waters yielded five different genomovars, i.e. B. cepacia (genomovar I), B. multivorans, B. cenocepacia, B. vietnamiensis and B. anthina. Drinking waters did not contain Bcc bacteria. However, the genomovar pattern from a given sample varied with the enrichment broth used.
authors.affiliation: Laboratory for Pharmaceutical Microbiology, University of Ghent, Ghent, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Vermis
authors.forename: Mariya
authors.initials: M
authors.lastname: Brachkova
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Hans
authors.initials: H
authors.lastname: Nelis
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001089:Arabinose / Q000378:metabolism; D042602:Burkholderia cepacia complex / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification; D005839:Gentamicins / Q000494:pharmacology; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011112:Polymyxin B / Q000494:pharmacology; D011938:Rec A Recombinases / Q000235:genetics; D012641:Selection, Genetic; D013912:Threonine / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D000166:Acridines; D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D005839:Gentamicins; D002475:Cellobiose; D013912:Threonine; C030016:9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan; D001089:Arabinose; D011938:Rec A Recombinases; D011112:Polymyxin B