Gleditsia sinensis fruit extract induced growth inhibition involves basic fibroblast growth factor and nitric oxide.
Recently we have shown the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various solid tumour and leukaemia cell lines as well as primary cultured bone marrow cells isolated from patients with acute and chronic myelogenous leukaemia. We further studied whether the growth inhibitory effect of GSE involves basic fibroblast growth factor (bFGF) in cancer cell lines including breast cancer MDA-MB231, nasopharyngeal cancer CNE-2 and prostate cancer LNCaP. We also investigated whether GSE could alter the production of nitric oxide (NO) pattern from these cancer cell lines. Growth inhibition assay was quantitated by sulforhodamine B protein staining method. Enzyme linked immunosorbent assay (ELISA) was used to quantitate the total bFGF protein. The amount of NO secreted into culture medium in terms of nitrite ion concentration was measured by the Greiss method. ELISA showed that GSE could stimulate total bFGF protein level which was dose- dependent. NO production was also stimulated from these cancer cell lines after treating with GSE. Both of the increment in total bFGF and NO levels were correlated with the degree of growth inhibition. Changes involving cell shrinkage and detachment of cancer cells could readily be observed. Taken together, our results here suggest that growth inhibition induced by GSE in these solid tumour cell lines may involve both bFGF and NO regulations.
Chui
Chung Hin
CH
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China.
Tang
Johnny Cheuk On
JC
Lau
Fung Yi
FY
Teo
Ivy Tuang Ngo
IT
Yau
Mable Yin Chun
MY
Wong
Raymond Siu Ming
RS
Cheng
Gregory Yin Ming
GY
Ho
Stephen King Wah
SK
Leung
Thomas Wai Tong
TW
Hui
Kin Shing
KS
Wong
Mai Man
MM
Fatima
Sarwat
S
Cheng
Chor Hing
CH
Cheung
Filly
F
Tan
Wei Qi
WQ
Chow
Larry Ming Cheung
LM
Guo
Dean
D
Chan
Albert Sun Chi
AS
D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism*; D031501:Gleditsia / Q000378:metabolism*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D009569:Nitric Oxide / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology
D006131:Growth Inhibitors; D010936:Plant Extracts; D016222:Fibroblast Growth Factor 2; D009569:Nitric Oxide