title: Aqueous oxygen attenuation of reperfusion microvascular ischemia in a canine model of myocardial infarction.
abstract: Uncorrected microvascular ischemia may contribute to left ventricular impairment during reperfusion after prolonged coronary artery occlusion. Attenuation of such ischemia in microvessels with impaired erythrocyte flow may require delivery of oxygen at high levels in plasma. Intraarterial infusion of aqueous oxygen (AO) can be used in a site specific manner to achieve hyperoxemic levels of oxygenation in the perfusate. With this new approach, the hypothesis was tested that reperfusion microvascular ischemia can be attenuated. After a 90 min coronary balloon occlusion in a canine model, AO hyperoxemic intracoronary perfusion was performed for 90 min after a 30 min period of autoreperfusion. Control groups consisted of normoxemic reperfusion, both passive (autoreperfusion) and active (roller pump). A significant improvement in left ventricular ejection fraction (p < 0.05) at 2 hr of reperfusion was noted only in the AO hyperoxemia group (17 +/- 6% by two dimensional echo), without a significant reduction in the improvement 1 hr after termination of treatment. During AO hyperoxemic perfusion, ECG ST segment isoelectric deviation normalized, and frequency of ventricular premature contractions was significantly reduced, in contrast to the autoreperfusion control group (p < 0.05). Microvascular blood flow, measured as the ischemic/normal left ventricular segment ratio by radiolabeled microspheres immediately after AO hyperoxemic perfusion, was double the value of the autoreperfusion control group at 2 hr of reperfusion (p < 0.05). We conclude that reperfusion microvascular ischemia is attenuated by intracoronary AO hyperoxemic perfusion and acutely improves left ventricular function in this model.
authors.affiliation: Cardiovascular Research Laboratory, Wayne State University, Department of Medicine, Detroit, Michigan 48201, USA.
authors.forename: J Richard
authors.initials: JR
authors.lastname: Spears
authors.forename: Petar
authors.initials: P
authors.lastname: Prcevski
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Giles
authors.initials: G
authors.lastname: Brereton
authors.forename: Marcello
authors.initials: M
authors.lastname: DiCarli
authors.forename: Ali
authors.initials: A
authors.lastname: Spanta
authors.forename: Richard
authors.initials: R
authors.lastname: Crilly
authors.forename: Steven
authors.initials: S
authors.lastname: Lavine
authors.forename: Richard
authors.initials: R
authors.lastname: vander Heide
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D004195:Disease Models, Animal; D004285:Dogs; D004562:Electrocardiography; D008833:Microcirculation / Q000187:drug effects; D009203:Myocardial Infarction / Q000175:diagnosis / Q000188:drug therapy*; D017202:Myocardial Ischemia / Q000175:diagnosis / Q000188:drug therapy*; D015425:Myocardial Reperfusion / Q000379:methods*; D010100:Oxygen / Q000008:administration & dosage* / Q000097:blood; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D010100:Oxygen