title: Analytical method for the determination of atrazine and its dealkylated chlorotriazine metabolites in water using SPE sample preparation and GC-MSD analysis.
abstract: A method is reported for the determination of atrazine and its dealkylated chlorotriazine metabolites in ground, surface, and deionized water. Water samples are adjusted to pH 3-4 prior to loading onto two SPE cartridges in series: C-18 and C-18/cation exchange mixed-mode polymeric phases. The analytes are eluted from each of the two cartridges separately, and the pooled and concentrated fraction is analyzed using gas chromatography-mass selective detection in the selected ion monitoring mode. The lower limit of method validation is 0.10 micrograms/L (ppb) for 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine), 2-amino-4-chloro-6-(isopropylamino)-s-triazine (G-30033, deethylatrazine), 2-amino-4-chloro-6-(ethylamino)-s-triazine (G-28279, deisopropylatrazine), and 2,4-diamino-6-chloro-s-triazine (G-28273, didealkyatrazine). The overall mean procedural recoveries (and standard deviations) are 96 (6.9), 96 (5.5), 95 (6.8), and 100% (10%) for atrazine, G-30033, G-28279, and G-28273, respectively (n = 49). The method validation study was conducted under U.S. EPA FIFRA Good Laboratory Practice Guidelines 40 CFR 160. The reported procedure accounts for residues of G-28273 in water.
authors.affiliation: Analytical Resources Group/Technology Support Department, Syngenta Crop Protection, Inc., Greensboro, North Carolina 27419-8300.
authors.forename: Sung-Ben
authors.initials: SB
authors.lastname: Huang
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Stanton
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Robert A
authors.initials: RA
authors.lastname: Yokley
mesh_terms: D001280:Atrazine / Q000032:analysis* / Q000378:metabolism; D006540:Herbicides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D010573:Pesticide Residues / Q000032:analysis; D012680:Sensitivity and Specificity; D014227:Triazines / Q000032:analysis* / Q000378:metabolism; D014867:Water / Q000737:chemistry*
chemical_list: D006540:Herbicides; D010573:Pesticide Residues; D014227:Triazines; D014867:Water; C060537:chlortriazine; D001280:Atrazine