[Production and characterization of a panel of monoclonal antibodies to Toxoplasma gondii antigens].
A representative collection was obtained containing 68 monoclonal antibodies (MAB) to Toxoplasma gondii antigens, which was characterized by the binding with the below fractions of tochizoites in the immune-enzyme assay (IEA) and immunoblotting (IB): membrane (MEM), somatic (water-soluble, SOM) and excretory-secretory (ES). Most of MABs were produced to MEM antigens (43), 6 MABs reacted with the somatic fraction, and 3 MABs reacted with both fractions. Two MABs to ES antigen were detected in the latter group. An analysis of MABs in concurrent IEA and IB revealed the immune-dominant proteins of the MEM and SOM fractions of antibodies to T. gondii tochizoites (p30 and p27, respectively). The presence of 2 non-overlapping antigenic determinants was shown for p30. Further research would detect MABs that could be used in the diagnosis of toxoplasmosis.
Poryvaeva
V A
VA
Vector-Best Ltd., Koltsovo, Novosibirsk Region, Russia.
Aborneva
I V
IV
Bormotov
N I
NI
Gladkova
S E
SE
Reshetnikov
S S
SS
Kuvshinova
I N
IN
Rukavishnikov
M Iu
MIu
Dedkova
L M
LM
Belanov
E F
EF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes