Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak.
The relation between the role of the organizer at the gastrula stage and the activity of earlier signals in the specification, maintenance, and regionalization of the developing brain anlage is still controversial. Mouse embryos homozygous for null mutation in the cripto gene die at about 9.0 days postcoitum (d.p.c.) and fail to gastrulate and to form the node (the primary organizer). Here, we study the presence and the distribution of anterior neural plate molecular domains in cripto null mutants. We demonstrate that, in cripto(-/-) embryos, the main prosencephalic and mesencephalic regions are present and that they assume the correct topological organization. The identity of the anterior neural domains is maintained in mutant embryos at 8.5 d.p.c., as well as in mutant explants dissected at 8.5 d.p.c. and cultured in vitro for 24 h. Our data imply the existence of a stable neural regionalization of anterior character inside the cripto(-/-) embryos, despite the failure in both the gastrulation process and node formation. These results suggest that, in mouse embryos, the specification of the anterior neural identities can be maintained without an absolute requirement for the embryonic mesoderm and the node.
Liguori
Giovanna L
GL
Institute of Genetics and Biophysics "A. Buzzati-Traverso", CNR, Via Guglielmo Marconi 12, 80125 Naples, Italy. liguori@iigb.na.cnr.it
Echevarría
Diego
D
Improta
Raffaele
R
Signore
Massimo
M
Adamson
Eileen
E
Martínez
Salvador
S
Persico
M Graziella
MG
D000818:Animals; D004475:Ectoderm / Q000502:physiology; D004815:Epidermal Growth Factor; D005260:Female; D005775:Gastrula / Q000502:physiology*; D008562:Membrane Glycoproteins; D008636:Mesencephalon / Q000196:embryology; D008648:Mesoderm / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000502:physiology*; D009420:Nervous System / Q000196:embryology*; D020897:Organizers, Embryonic / Q000502:physiology*; D016548:Prosencephalon / Q000196:embryology
D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C489450:Tdgf1 protein, mouse; D004815:Epidermal Growth Factor