[Expression of telomerase and p53 in ameloblastoma].
OBJECTIVE
To detect the expression of p53 protein, hTR, and hTERT mRNA in ameloblastoma (AB) and to investigate the clinical biological characteristics of AB.


METHODS
We detected hTR and hTERT Mrna in 54 cases of AB by in situ hybridization, and p53 protein by S-P method.


RESULTS
The positive ratio of hTR, hTERT mRNA was 2/7 and 1/7 in normal oral mucosa, 81.2%(13/16) and 87.4% (14/16) in odontogenic keratocyst (OKC), 94.4%(51/54) in AB, respectively. There was a significant difference in these three groups (P < 0.001). A strong correlation between hTR and hTERT mRNA was found (rs = 1.000, P < 0.001). p53 protein was expressed in 87.5% (42/49) AB, 44%(11/25) OKC, 33.3%(1/3) normal oral mucosa, respectively. A strong correlation between hTERT mRNA and p53 protein was found (rs = 0.848, P < 0.001). A moderate correlation between hTR mRNA and p53 protein was found (rs = 0.536, P < 0.001).


CONCLUSIONS
The positive ratios of hTR and hTERT were higher than p53 protein in AB. Telomerase activity and p53 expression play an important role in genesis and development of AB.
Zhong
Ming
M
College of Stomatology, China Medical University, Shenyang 110002, China. zhongming1234@163.net
Zhang
Lu-huang
LH
Wang
Jie
J
Zhang
Bo
B
Hou
Lin
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000737:chemistry* / Q000209:etiology; D002648:Child; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007573:Jaw Neoplasms / Q000737:chemistry* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000032:analysis* / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019098:Telomerase