Screening for multiple hereditary hypercoagulability factors using the amplification refractory mutation system.
Many hereditary factors have been implicated in the development of arterial and/or venous thromboembolic diseases. A number of these risk factors can be identified by the amplification refractory mutation system (ARMS). However, the underlying technical conditions for performing ARMS are highly variable, and depend on which risk factors are being analyzed. We have now developed a novel ARMS-based system to simultaneously screen for multiple hypercoagulability factors under identical PCR conditions. This can greatly simplify the process of screening for hereditary hypercoagulability.
Hoppe
Berthold
B
Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin Campus Virchow-Klinikum, Humboldt-Universität Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. berthold.hoppe@charite.de
Heymann
Guido A
GA
Koscielny
Jürgen
J
Hellstern
Peter
P
Kiesewetter
Holger
H
Salama
Abdulgabar
A
D001483:Base Sequence; D001778:Blood Coagulation Disorders / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics*; D001779:Blood Coagulation Factors / Q000235:genetics; D001798:Blood Proteins / Q000235:genetics; D017931:DNA Primers; D006580:Genetic Carrier Screening; D006720:Homozygote; D006801:Humans; D008403:Mass Screening / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods
D001779:Blood Coagulation Factors; D001798:Blood Proteins; D017931:DNA Primers