[Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment].
OBJECTIVE
To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.


METHODS
Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.


RESULTS
The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.


CONCLUSIONS
Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts
Zhan
Yu-tao
YT
Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Wei
Lai
L
Chen
Hong-song
HS
Cong
Xu
X
Fei
Ran
R
Wang
Yu
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology*; D004777:Environment; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D022781:Hepatocytes / Q000473:pathology*; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley