Protein crystal growth with a two-liquid system and stirring solution.
We developed two novel methods for growing large, high-quality protein crystals. A two-liquid system enables the convenient extraction of protein crystals without causing mechanical damage due to growth at the interface between two liquids. Since this system does not require limitations on solution volume, it is also suitable for the seed technique, and for growing large crystals. Another new concept is the mild stirring of the solution using the Floating And Stirring Technique (FAST) and the Micro-stirring technique. When compared to conventional techniques, both techniques result in a reduced number of crystals, as well as the growth of large crystals.
Adachi
Hiroaki
H
Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. adachi@pwr.eng.osaka-u.ac.jp
Takano
Kazufumi
K
Matsumura
Hiroyoshi
H
Inoue
Tsuyoshi
T
Mori
Yusuke
Y
Sasaki
Takatomo
T
D002498:Centrifugation / Q000295:instrumentation* / Q000379:methods*; D003460:Crystallization / Q000295:instrumentation* / Q000379:methods*; D004058:Diffusion; D004867:Equipment Design; D019544:Equipment Failure Analysis; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009113:Muramidase / Q000138:chemical synthesis / Q000737:chemistry / Q000648:ultrastructure; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000648:ultrastructure; D012996:Solutions
D011506:Proteins; D012996:Solutions; C421864:hen egg lysozyme; D009113:Muramidase