Vacuolar H+ pumping ATPases in luminal acidic organelles and extracellular compartments: common rotational mechanism and diverse physiological roles.
Cytoplasmic organelles with an acidic luminal pH include vacuoles, coated vesicles, lysosomes, the Golgi apparatus, and synaptic vesicles. Acidic compartments are also known outside specialized cells such as osteoclasts. The unique acidic pH is formed by V-ATPase (Vacuolar type ATPase), other ion transporters, and the buffering action of proteins inside the organelles. V-ATPase hydrolyzes ATP and transports protons inside an organelle or extracellular compartment. We have summarized recent progress on mouse V-ATPases and their varying localizations together with their mechanism emphasizing similarities with F-type ATPases.
Sun-Wada
Ge-Hong
GH
Division of Biological Sciences and Nanoscience, and Nanotechnology Center, Japan Science and Technology Cooperation, Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Wada
Yoh
Y
Futai
Masamitsu
M
D000818:Animals; D002384:Catalysis; D005110:Extracellular Space / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D020409:Molecular Motor Proteins; D015388:Organelles / Q000201:enzymology; D021122:Protein Subunits / Q000502:physiology; D012399:Rotation; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry* / Q000502:physiology*
D020409:Molecular Motor Proteins; D021122:Protein Subunits; D025262:Vacuolar Proton-Translocating ATPases