Structure-function relationships in enzymatically modified articular cartilage.
The present study is aimed at revealing structure-function relationships of bovine patellar articular cartilage. Collagenase, chondroitinase ABC and elastase were used for controlled and selective enzymatic modifications of cartilage structure, composition and functional properties. The effects of the enzymatic degradations were quantitatively evaluated using quantitative polarized light microscopy, digital densitometry of safranin O-stained sections as well as with biochemical and biomechanical techniques. The parameters related to tissue composition and structure were correlated with the indentation stiffness of cartilage. In general, tissue alterations after enzymatic digestions were restricted to the superficial cartilage. All enzymatic degradations induced superficial proteoglycan (PG) depletion. Collagenase also induced detectable superficial collagen damage, though without causing cartilage fibrillation or tissue swelling. Quantitative microscopic techniques were more sensitive than biochemical methods in detecting these changes. The Young's modulus of cartilage decreased after enzymatic treatments indicating significant softening of the tissue. The PG concentration of the superficial zone proved to be the major determinant of the Young's modulus (r(2) = 0.767, n = 72, p < 0.001). Results of the present study indicate that specific enzymatic degradations of the tissue PGs and collagen can provide reproducible experimental models to clarify the structure-function relationships of cartilage. Effects of these models mimic the changes observed in early osteoarthrosis. Biomechanical testing and quantitative microscopic techniques proved to be powerful tools for detecting the superficial structural and compositional changes while the biochemical measurements on the whole uncalcified cartilage were less sensitive.
Rieppo
Jarno
J
Department of Anatomy, University of Kuopio, Kuopio, Finland. Heikki.Helminen@uku.fi
Töyräs
Juha
J
Nieminen
Miika T
MT
Kovanen
Vuokko
V
Hyttinen
Mika M
MM
Korhonen
Rami K
RK
Jurvelin
Jukka S
JS
Helminen
Heikki J
HJ
D000818:Animals; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000378:metabolism / Q000502:physiology*; D002417:Cattle; D019765:Chondroitin ABC Lyase; D017364:Collagenases; D008297:Male; D008859:Microscopy, Polarization; D010196:Pancreatic Elastase; D010329:Patella; D011509:Proteoglycans / Q000378:metabolism; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship
D011509:Proteoglycans; D010196:Pancreatic Elastase; D017364:Collagenases; D019765:Chondroitin ABC Lyase