Galectin-1, a cell adhesion modulator, induces apoptosis of rat Leydig cells in vitro.
Galectin-1 (Gal-1), a beta galactoside-binding lectin, is involved in multiple biological functions, such as cell adhesion, apoptosis, and metastasis. On the basis of its ability to interact with extracellular matrix (ECM) glycoproteins, we investigated the Gal-1 effect on Leydig cells, which express and are influenced by ECM proteins. In this study, Gal-1 was identified in Leydig cell cultures by immunofluorescence. To gain insight into its biological role, Gal-1 was added to purified rat Leydig cells, under both basal and human chorionic gonadotrophin-stimulated conditions. Substantial morphological changes were observed, and cell viability showed an 80% decrease after 24 h culture. As a functional consequence of Gal-1 addition, testosterone production was reduced in a dose-dependent fashion, reaching a minimum of 26% after 24 h compared with basal values. cAMP showed a similar variation after 3 h. Assessment of DNA hypodiploidy and caspase activity determinations indicated that the reduction in viability and in steroidogenesis was caused by apoptosis induced by Gal-1. Besides, addition of Gal-1 caused Leydig cell detachment. Presence of laminin-1 or lactose prevented the effect of Gal-1, suggesting that the carbohydrate recognition domain is involved in inducing apoptosis. These findings demonstrate a novel mechanism, based on Gal-1 and laminin-1 interaction, which could help us better understand the molecular basis of Leydig cell function and survival control.
Martinez
Vanesa G
VG
Instituto de Química y Fisicoquómica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquómica, Universidad de Buenos Aires, Junón 956, (1113) Buenos Aires, Argentina.
Pellizzari
Eliana H
EH
Díaz
Emilce S
ES
Cigorraga
Selva B
SB
Lustig
Livia
L
Denduchis
Berta
B
Wolfenstein-Todel
Carlota
C
Iglesias
M Mercedes
MM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D003470:Culture Media; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D037483:Galectin 1 / Q000494:pharmacology*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D003470:Culture Media; D037483:Galectin 1; D013739:Testosterone; C505407:CASP3 protein, human; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases