Production of polyacrylamide gradient gel for lipoprotein electrophoretic separation.
BACKGROUND
Small LDL are associated with risk of coronary heart disease. Gradient gel electrophoresis for LDL separation is not a simple method and high-quality non-denaturing gradient gels are lacking.


METHODS
In this paper, we describe a method for the preparation of a polyacrylamide gel system that consists of an upper linear gradient gel (1.8-10%) and a lower homogeneous gel (16%) for the determination of LDL size.


RESULTS
The linear gradient is highly reproducible. Intra-inter gel coefficients of variation for LDL particle size are lower than 0.6%.


CONCLUSIONS
Effective LDL size measurement from pre-stained serum samples is possible in a stable gel.
Fonda
Maurizio
M
Department of Clinical, Morphologic and Technological Sciences, Clinica Medica, University of Trieste, Cattinara Hospital, Strada di Fiume, 34149 Trieste, Italy. fondam@units.it
Semolic
Anna M
AM
Soranzo
Maria R
MR
Cattin
Luigi
L
D000180:Acrylic Resins / Q000138:chemical synthesis*; D004586:Electrophoresis / Q000295:instrumentation*; D006801:Humans; D016014:Linear Models; D017270:Lipoprotein(a) / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D015203:Reproducibility of Results
D000180:Acrylic Resins; D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; C016680:polyacrylamide gels