Genomic structure and characterisation of the promoter region of the human IL-18 gene.
Interleukin (IL)-18 is a cytokine that plays an important role in the T helper (Th) 1 response, primarily by its ability to induce gamma interferon (IFN-gamma) production by T cells and Natural Killer (NK) cells. It also plays a role in host resistance to infection and in Lipopolysaccharides (LPS)-induced histopathology. By a direct sequencing on P1 Artificial Chromosomes (PAC) clones, we have determined the genomic structure and the promoter region of the human IL-18 gene. The IL-18 gene spans approximately 20 Kb and consists of 6 exous. The 5'-flanking regions of human IL-18 and mouse IL-18 show 75% homology suggesting conserved promoter regulatory factors.
el Kares
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R
Laboratoire d'Immunologie Vaccinologie et Génétique Moléculaire, Institut Pasteur de Tunis, 13, Place Pasteur-BP.74, 1002 Tunis Belvedere.
Abdelhak
S
S
Dellagi
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K
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D005091:Exons / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology; D020382:Interleukin-18 / Q000235:genetics*; D007694:Killer Cells, Natural / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013601:T-Lymphocytes / Q000502:physiology; D018417:Th1 Cells / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics
D020382:Interleukin-18; D014157:Transcription Factors; D007371:Interferon-gamma