title: [An immunohistochemical study on neovascularization in TMJ during mandibular advancement].
abstract: OBJECTIVE
  Neovascularization is proved to be closely correlated with ossification. This study was designed to determine the biochemical path through which the new bone formation is enhanced in TMJ during mandibular protrusion.
  
  
  METHODS
  SD Rats were fitted with bite-jumping appliances to allow for an adequate advancement of the mandible. The animals were sacrificed 3, 7, 14, 21 and 30 days, respectively, after placement of the appliances. Slides with TMJ tissue were prepared for biochemical procedure. Immunohistochemical approaches were adopted to examine neovascularization in TMJ by immuno-localizing the newly formed endothelial cells. Computer-assisted image analysis system was applied to quantify the positive immunoreaction.
  
  
  RESULTS
  Neovascularization in connective tissue close to the condyle was 200% higher in the experimental groups than that in the control, and, within the bony tissue of the condyle, neovascularization in experimental animals was found to be 103% higher than that of the control.
  
  
  CONCLUSIONS
  Increased neovascularization triggered by mandibular protrusion might intensify new bone formation in condyle and glenoid fossa and finally lead to a growth adaptation of TMJ to bite-jumping therapy.
authors.affiliation: Department of Orthodontics, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China. gangshen@orthosh.com
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: A B
authors.initials: AB
authors.lastname: Rabie
authors.forename: U
authors.initials: U
authors.lastname: Hägg
authors.forename: Rong-jing
authors.initials: RJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D019186:Mandibular Advancement; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D013704:Temporomandibular Joint / Q000098:blood supply*