Comparison of proteome between hepatitis B virus- and hepatitis C virus-associated hepatocellular carcinoma.
OBJECTIVE
Hepatocellular carcinoma (HCC) is one of the most common malignant cancers closely associated with chronic infection by the hepatitis B virus (HBV) or the hepatitis C virus (HCV) throughout the world. Differential expression of the proteome in HBV- and HCV-associated HCC was investigated to identify any useful biomarkers indicating virus-specific hepatocarcinogenesis.


METHODS
Twenty-one pairs of specimens (tumorous and surrounding nontumorous liver tissues) were obtained from 21 HCC patients. They were divided into three HCC types by viral markers: 7 hepatitis B surface antigen-positive (B-type HCC), 7 anti-HCV-positive (C-type HCC), and 7 hepatitis B surface antigen-negative and anti-HCV-negative. Total proteins were analyzed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and alterations in the proteome were examined.


RESULTS
Sixty proteins were identified that show significant changes in the expression level between nontumorous and tumorous tissues. Among these, 14 proteins were commonly changed in all three of the HCC types, but 46 proteins showed a tendency of viral marker specificity.


CONCLUSIONS
The identified proteins were classified according to the viral factor as being involved in B-type and C-type HCC. These results suggest strongly that the expression pattern of proteome in HCC tissues is closely associated with etiologic factors. The different protein profiles between B-type and C-type HCC indicate that the pathogenetic mechanisms of hepatocarcinogenesis may be different according to the viral factor, HBV and HCV.
Kim
Won
W
Schools of Biological Sciences and. Chemistry and Molecular Engineering, Seoul National University, Seoul, Korea.
Oe Lim
Seung
S
Kim
Jong-Seo
JS
Ryu
Young Hee
YH
Byeon
Ji-Yeon
JY
Kim
Hie-Joon
HJ
Kim
Yong-Il
YI
Heo
Jin Seok
JS
Park
Young Min
YM
Jung
Guhung
G
D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology*; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006509:Hepatitis B / Q000150:complications; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology*; D020543:Proteome / Q000145:classification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry / Q000145:classification
D020543:Proteome; D014764:Viral Proteins