Determination of amino acids in cell culture and fermentation broth media using anion-exchange chromatography with integrated pulsed amperometric detection.
Cell culture and fermentation broth media are used in the manufacture of biotherapeutics and many other biological materials. Characterizing the amino acid composition in cell culture and fermentation broth media is important because deficiencies in these nutrients can reduce desired yields or alter final product quality. Anion-exchange (AE) chromatography using sodium hydroxide (NaOH) and sodium acetate gradients, coupled with integrated pulsed amperometric detection (IPAD), determines amino acids without sample derivatization. AE-IPAD also detects carbohydrates, glycols, and sugar alcohols. The presence of these compounds, often at high concentrations in cell culture and fermentation broth media, can complicate amino acid determinations. To determine whether these samples can be analyzed without sample preparation, we studied the effects of altering and extending the initial NaOH eluent concentration on the retention of 42 different carbohydrates and related compounds, 30 amino acids and related compounds, and 3 additional compounds. We found that carbohydrate retention is impacted in a manner different from that of amino acid retention by a change in [NaOH]. We used this selectivity difference to design amino acid determinations of diluted cell culture and fermentation broth media, including Bacto yeast extract-peptone-dextrose (yeast culture medium) broth, Luria-Bertani (bacterial culture medium) broth, and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture media). These media were selected as representatives for both prokaryotic and eukaryotic culture systems capable of challenging the analytical technique presented in this paper. Glucose up to 10mM (0.2%, w/w) did not interfere with the chromatography, or decrease recovery greater than 20%, for the common amino acids arginine, lysine, alanine, threonine, glycine, valine, serine, proline, isoleucine, leucine, methionine, histidine, phenylalanine, glutamate, aspartate, cystine, and tyrosine.
Hanko
Valoran P
VP
Dionex Corp, 500 Mercury Drive, Sunnyvale, CA 94088-3603, USA. val.hanko@dionex.com
Rohrer
Jeffrey S
JS
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000838:Anions; D001419:Bacteria / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis*; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D005057:Eukaryotic Cells / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000032:analysis; D006018:Glycols / Q000032:analysis; D011387:Prokaryotic Cells / Q000378:metabolism; D012972:Sodium Hydroxide; D013402:Sugar Alcohols / Q000032:analysis; D013997:Time Factors; D015003:Yeasts / Q000378:metabolism
D000596:Amino Acids; D000838:Anions; D002241:Carbohydrates; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006018:Glycols; D013402:Sugar Alcohols; D012972:Sodium Hydroxide; D005947:Glucose