The Bcl-2 protein expression in germinal centers of hypertrophied adenoids in children.
The aim of this study was to evaluate Bcl-2 expression in the germinal centers of adenoid in children on the assumption that it can be treated as a marker of adenoidal tissue function. The study involved 95 children. The patients were divided into three age groups: aged up to 5, 5-10 and above 10 years. The analysed material were adenoids removed on the grounds of hypertrophy. Immunohistochemical analyses were carried out using monoclonal antibodies (DAKO/Bcl-2, No. M0887) directed against human Bcl-2 protein. The presence of Bcl-2 positive lymphocytes within the lymphoid follicles and Bcl-2 immunostaining were scored. The immunohistochemical staining showed Bcl-2 positive lymphocytes in the examined sections of adenoidal tissue and their characteristic location, mainly within the mantle zone. Bcl-2 reactivity was widespread in hyperplastic lymphoid tissue, including germinal centers surroundings, scattered cells within the germinal centers and the T-cell areas in general. We have not found statistically significant correlation between Bcl-2 expression and clinical status and change in Bcl-2 expression level according to age. The apoptosis presence within the germinal centers and its lack in mantle zone, the manifestation of which is Bcl-2 expression, is a sign of a proper lymphocytes maturation within lymphoid follicles. It seems that these processes are not influenced by age, so the adenoid involution is probably associated with the changes in the number of lymphoid follicles rather than change or inhibition of the processes that take place in them.
Musiatowicz
M
M
Department of Paediatric Otorhinolaryngology, Medical University of Bialystok, Waszyngtona 17, 15-273 Bialystok, Poland. medix@home.pl
Hassmann-Poznańska
E
E
Musiatowicz
B
B
Baltaziak
M
M
D000234:Adenoids / Q000378:metabolism / Q000473:pathology*; D000911:Antibodies, Monoclonal / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics*; D018858:Germinal Center / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006984:Hypertrophy / Q000235:genetics / Q000473:pathology; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000473:pathology; D008224:Lymphoma, Follicular / Q000473:pathology; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D019253:Proto-Oncogene Proteins c-bcl-2