title: Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929.
abstract: Harpagophytum procumbens (Pedaliaceae) has been used for the treatment of pain and arthritis. The effect of Harpagophytum procumbens against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2) (PGE(2)) immunoassay, and nitric oxide detection on mouse fibroblast cell line L929. The aqueous extract of Harpagophytum procumbens was shown to suppress PGE(2) synthesis and nitric oxide production by inhibiting lipopolysaccharide-stimulated enhancement of the cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) mRNAs expressions in L929 cells. These results suggest that Harpagophytum procumbens exerts anti-inflammatory and analgesic effects probably by suppressing cyclooxygenase-2 and iNOS expressions.
authors.affiliation: Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Sabina
authors.initials: S
authors.lastname: Lim
authors.forename: Seung-Moo
authors.initials: SM
authors.lastname: Han
authors.forename: Hi-Joon
authors.initials: HJ
authors.lastname: Park
authors.forename: Insop
authors.initials: I
authors.lastname: Shin
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Nam-Jae
authors.initials: NJ
authors.lastname: Kim
authors.forename: Ji-Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology; D031684:Harpagophytum; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis
chemical_list: D007527:Isoenzymes; D008070:Lipopolysaccharides; D010936:Plant Extracts; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases