Muscle enzyme activities in a deep-sea squaloid shark, Centroscyllium fabricii, compared with its shallow-living relative, Squalus acanthias.
The activities of several enzymes of energy metabolism were measured in the heart, red muscle, and white muscle of a deep and a shallow living squaloid shark, Centroscyllium fabricii and Squalus acanthias, respectively. The phylogenetic closeness of these species, combined with their active predatory nature, similar body form, and size makes them well matched for comparison. This is the first time such a comparison has been made involving a deep-sea elasmobranch. Enzyme activities were similar in the heart, but generally lower in the red muscle of C. fabricii. Paralleling the trend seen in deep-sea teleosts, the white muscle of C. fabricii had substantially lower activities of key glycolytic enzymes, pyruvate kinase and lactate dehydrogenase, relative to S. acanthias or other shallow living elasmobranchs. Unexpectedly, between the squaloid sharks examined, creatine phosphokinase activity was higher in all tissues of the deep living C. fabricii. Low white muscle glycolytic enzyme activities in the deep-sea species coupled with high creatine phosphokinase activity suggests that the capacity for short burst swimming is likely limited once creatine phosphate supplies have been exhausted.
Treberg
Jason R
JR
Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, Newfoundland, A1C 5S7, Canada. jtreberg@mun.ca
Martin
R Aidan
RA
Driedzic
William R
WR
D000818:Animals; D001267:Atlantic Ocean; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D004777:Environment; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D009523:Newfoundland and Labrador; D011770:Pyruvate Kinase / Q000378:metabolism; D012754:Sharks / Q000502:physiology*; D013053:Spectrophotometry
D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D003402:Creatine Kinase