title: Purification and characterization of an aldehyde oxidase from Pseudomonas sp. KY 4690.
abstract: An aldehyde oxidase, which oxidizes various aliphatic and aromatic aldehydes using O(2) as an electron acceptor, was purified from the cell-free extracts of Pseudomonas sp. KY 4690, a soil isolate, to an electrophoretically homogeneous state. The purified enzyme had a molecular mass of 132 kDa and consisted of three non-identical subunits with molecular masses of 88, 39, and 18 kDa. The absorption spectrum of the purified enzyme showed characteristics of an enzyme belonging to the xanthine oxidase family. The enzyme contained 0.89 mol of flavin adenine dinucleotide, 1.0 mol of molybdenum, 3.6 mol of acid-labile sulfur, and 0.90 mol of 5'-CMP per mol of enzyme protein, on the basis of its molecular mass of 145 kDa. Molecular oxygen served as the sole electron acceptor. These results suggest that aldehyde oxidase from Pseudomonas sp. KY 4690 is a new member of the xanthine oxidase family and might contain 1 mol of molybdenum-molybdpterin-cytosine dinucleotide, 1 mol of flavin adenine dinucleotide, and 2 mol of [2Fe-2S] clusters per mol of enzyme protein. The enzyme showed high reaction rates toward various aliphatic and aromatic aldehydes and high thermostability.
authors.affiliation: Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyo 3-Chome, Fukui-shi 910-8507, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Uchida
authors.forename: Daisaku
authors.initials: D
authors.lastname: Kondo
authors.forename: Ayako
authors.initials: A
authors.lastname: Yamashita
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nagaosa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakurai
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujii
authors.forename: Kinya
authors.initials: K
authors.lastname: Fujishiro
authors.forename: Kazuo
authors.initials: K
authors.lastname: Aisaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Uwajima
mesh_terms: D042931:Aldehyde Oxidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D006358:Hot Temperature; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D042931:Aldehyde Oxidase