title: [Relationship of apoptosis and expression of Fas gene in tongue squamous cell carcinoma cell line Tca8113].
abstract: OBJECTIVE
  To investigate the relationship of apoptosis and expression of Fas gene in oral squamous cell carcinoma.
  
  
  METHODS
  Plasmid including Fas gene was transfected into Tca8113 cell by lipofectamine kit. The expression of Fas mRNA in transfected and non-transfected cell was detected by RT-PCR. The expression of Fas protein and apoptosis index(AI) was assessed by flow cytometry(FCM).
  
  
  RESULTS
  No variation of positive expression rate of Fas protein was found in Fas-transfected and non-transfected Tca8113 (P > 0.05), but the expression of Fas mRNA, intensity of Fas protein and apoptosis index were significantly increased respectively from 0.201 +/- 0.015, 31.02 +/- 4.63, 0.88% +/- 0.16% to 0.399 +/- 0.073, 54.32 +/- 6.38 and 10.21% +/- 1.46%(P < 0.01).
  
  
  CONCLUSIONS
  Apoptosis in tongue squamous cell carcinoma was not directly associated with the positive expression rate but intensity of Fas protein. A liminal value might exist before apoptosis in Tca8113 cell could be activated.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Hong-zhang
authors.initials: HZ
authors.lastname: Huang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Hou
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Cheng
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D019014:fas Receptor