title: Clinical aspects for the use of DNA image cytometry in detection of bladder cancer: a valuable tool?
abstract: The employment of DNA flow or image cytometry for oncological diagnostic procedures is favored because of its high correlation to tumor biological behavior. Prognostic statements and therapeutic strategies therefore are based on the high validity of DNA cytometric measurements. Using 151 bladder washings from patients suspected of bladder cancer for this study, we examined the clinical value of various common methods of DNA single cell (SCI) and stemline interpretations (SLI). Comparing the specificity and sensitivity of DNA image cytometry in detection of bladder tumors, we found 81 and 52%, respectively, for SCI of Boecking, 84 and 45% for SLI of Boecking, 61 and 58% for SLI of Fu, and 82 and 40% for conventional stemline interpretation. To improve diagnostic and prognostic validity of DNA image cytometry, we designed our own method of interpretation. In consequence, we identified six single DNA parameters out of all recorded measurements that correlated most to histopathological grading (G1-G3). Creating reference values at random and rating by points, we used a cytometric grading system for ranking. In detection of bladder cancer specificity and sensitivity ultimately arrived at almost 70% in application of our method. Thus, by this study, we were able to show that sensitivity of DNA examination can be increased by combining various DNA parameters. Apart from our own scheme, the discrepancy in interpretation of DNA image cytometry does not allow us to recommend this procedure as the only diagnostic in detection of bladder cancer. However, in regard to prognostic statements, particularly tumor biological behavior, DNA image cytometry appears to be useful.
authors.affiliation: Department of Urology, Friedrich-Alexander-University, Krankenhausstrasse 12, 91054 Erlangen, Germany. frens.steffen.krause@t-online.de
authors.forename: F Steffen
authors.initials: FS
authors.lastname: Krause
authors.forename: Gerhard
authors.initials: G
authors.lastname: Feil
authors.forename: Karl-H
authors.initials: KH
authors.lastname: Bichler
authors.forename: Karl M
authors.initials: KM
authors.lastname: Schrott
authors.forename: Ziya Y
authors.initials: ZY
authors.lastname: Akcetin
mesh_terms: D000782:Aneuploidy; D016022:Case-Control Studies; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D011003:Ploidies; D011379:Prognosis; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm