Cytochrome oxidase activity in the monkey globus pallidus and subthalamic nucleus after ablation of striatal interneurons expressing substance P receptors.
To understand functional roles of striatal interneurons in primate basal ganglia circuitry, we ablated interneurons expressing substance P (SP) receptors (SPR) in the putamen with SP-saporin, a SPR selective neurotoxin. The effect of SP-saporin injection into the putamen was evaluated by examining the loss of cholinergic interneurons and NADPHd-positive (nicotinamide adenine dinucleotide phosphate diaphorase positive) interneurons. We then analyzed regional metabolic changes using cytochrome oxidase (CO) histochemistry. CO activity in some regions of the internal and external segments of the globus pallidus (GP) in the lesioned hemisphere was lower than that in the contralateral or surrounding GP regions. CO activity in the subthalamic nucleus, however, showed no significant change. The present findings suggest that striatopallidal projection neurons exert enhanced inhibitory influence on the GP without modulatory control by the striatal SPR-expressing interneurons.
Chiken
Satomi
S
Department of Brain Structure, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan.
Hatanaka
Nobuhiko
N
Tokuno
Hironobu
H
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D007839:Functional Laterality; D005917:Globus Pallidus / Q000378:metabolism; D007150:Immunohistochemistry; D000922:Immunotoxins / Q000008:administration & dosage / Q000633:toxicity; D007276:Injections, Intraventricular; D007395:Interneurons / Q000187:drug effects* / Q000378:metabolism; D008251:Macaca; D008297:Male; D009699:N-Glycosyl Hydrolases / Q000008:administration & dosage / Q000633:toxicity; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009498:Neurotoxins / Q000008:administration & dosage / Q000633:toxicity; D010940:Plant Proteins / Q000008:administration & dosage / Q000633:toxicity; D018040:Receptors, Neurokinin-1 / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D020531:Subthalamic Nucleus / Q000378:metabolism
D000922:Immunotoxins; D009498:Neurotoxins; D010940:Plant Proteins; D018040:Receptors, Neurokinin-1; D054789:Ribosome Inactivating Proteins, Type 1; D009252:NADPH Dehydrogenase; D003576:Electron Transport Complex IV; D002795:Choline O-Acetyltransferase; D009699:N-Glycosyl Hydrolases; D000076985:Saporins