[Case report: Porcine circovirus type 2 infection in an European wild boar (Sus scrofa) in the state of Brandenburg, Germany].
This case represents the first case of Porcine Circovirus Type 2 (PCV-2)--infection in a free living European wild boar associated with morphological lesions, which are regarded as characteristic for Postweaning Multisystemic Wasting Syndrome (PMWS) in domestic pigs. The animal, an approximately 10 month old male, was found dead in a rural area within the state of Brandenburg, Germany. The closest commercial pig farm is located in 3 km distance from the spot where the carcass was found. At necropsy, the animal was found to be in a runted condition. Morphological investigation revealed two lesion complexes. Firstly, lymphatic depletion was present in different organs. Mainly the white pulp of the spleen was affected, where lymph follicles and periarteriolar lymphatic sheaths were nearly completely depleted of lymphoid cells. The former lymphatic areas could only be identified by the presence of histiocytic cells. Secondly, there were widely distributed lesions indicative of a bacterial septicemia i.e. purulent-necrotizing lymphadenitis, pulpous hyperplasia of the spleen, miliary lytic liver necroses and foci of fibrinous pneumonia. Within the lesions, bacterial colonies were found (short Gram-negative rods). Bacteriology revealed a septicemic Salmonella choleraesuis var. Kunzendorf--infection. Virologically, the animal was tested with negative results for Classical Swine Fever Virus and PRRSV. The unusual depletion of the lymphatic tissue mainly in the spleen led to the suspicion of a PCV-2 infection. Typical circoviral particles were found by negative-contrast electron microscopy in samples from spleen and lymph nodes. Using a commercial antiserum against Porcine Circovirus, positive staining was found by fluorescence microscopy in tonsils, spleen and lymph nodes. Finally, the virus was identified to be PCV-2 by species-specific PCR. The presented case rises the questions if PCV-2 is endemic in the European wild boar population at least in certain areas, if it is of pathogenetic importance for wild boars and if the virus present in wild boars is identical to that present in domestic pigs with PMWS.
Schulze
C
C
Landesamt für Verbraucherschutz und Landwirtschaft, Laborbereich Frankfurt (Oder)-Markendorf.
Neumann
G
G
Grütze
I
I
Engelhardt
A
A
Mirle
C
C
Ehlert
F
F
Hlinak
A
A
D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000302:isolation & purification; D005858:Germany / Q000453:epidemiology; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D034421:Sus scrofa; D013553:Swine Diseases / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D019282:Wasting Syndrome / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology