In vivo postirradiation protection by a vitamin E analog, alpha-TMG.
The water-soluble vitamin E derivative alpha-TMG is an excellent radical scavenger. A dose of 600 mg/kg TMG significantly reduced radiation clastogenicity in mouse bone marrow when administered after irradiation. The present study was aimed at investigating the radioprotective effect of postirradiation treatment with alpha-TMG against a range of whole-body lethal (8.5-12 Gy) and sublethal (1-5 Gy) doses of radiation in adult Swiss albino mice. Protection against lethal irradiation was evaluated from 30-day mouse survival and against sublethal doses was assessed from micronuclei and chromosomal aberrations in the bone marrow 24 h after irradiation. An intraperitoneal injection of 600 mg/kg TMG within 10 min of lethal irradiation increased survival, giving a dose modification factor (DMF) of 1.09. TMG at doses of 400 mg/kg and 600 mg/kg significantly reduced the percentage of aberrant metaphases, the different types of aberrations, and the number of micronucleated erythrocytes. DMFs of 1.22 and 1.48 for percentage aberrant metaphases and 1.6 and 1.98 for micronuclei were obtained for 400 mg/kg and 600 mg/kg TMG, respectively. No drug toxicity was observed at these doses. The effectiveness of TMG when administered postirradiation suggests its possible utility for protection against unplanned radiation exposures.
Satyamitra
Merriline
M
Department of Research, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal 462001, India.
Uma Devi
P
P
Murase
Hironobu
H
Kagiya
V T
VT
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001853:Bone Marrow / Q000528:radiation effects; D002839:Chromans / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*
C106323:2-(glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol; D000975:Antioxidants; D002839:Chromans; D006027:Glycosides; D011837:Radiation-Protective Agents