Study of transcripts from AC010088, a 199,485 bp fragment of the human Y chromosome located in the azoospermia factor region c.
Deletions on the long arm of the human Y chromosome are associated with male infertility. In this work, we studied transcripts of a 199,485 bp long fragment of the Yq11 region (GenBank accession number, AC010088) located in the AZFc (azoospermia factor region c), and characterized their gene structures. After masking repetitive elements, we searched human mRNA Refseqs (reference sequences), a dbEST (database of expressed sequence tags) and a non-redundant nucleic acid database for the mRNAs and ESTs corresponding to the AC010088 using the BLAST programs at the NCBI (National Center for Biotechnology Information) site. Our findings are summarized as follows: i) BPY2 (testis basic protein on Y, 2), DAZ1 (deleted in azoospermia 1), TTY4 (testis transcript Y 4) mRNAs and 23 ESTs were found; ii) Eighteen of 23 ESTs were transcripts of the DAZ gene(s), one EST was a transcript of TTY4 gene, and the remaining 4 probably corresponded to 4 different pseudogenes; iii) DAZ gene(s) were expressed not only in testis, but also in lung carcinoma cells, stomach and Ewing's sarcoma cells; iv) beta-satellite clusters were present around and within the BPY2 and TTY4 gene region; v) In this study, TTY4, BPY2 and DAZ1 genes were mapped precisely to the AC010088 region.
Kondo
Yuki
Y
Hirakata Ryoikuen, Tsuda-higashi 2-1-1, Hirakata, Osaka 573-0122, Japan.
Watanabe
Makoto
M
Nishigushi
Seiji
S
Shimda
Kazunori
K
Yamanishi
Hiromochi
H
Kurokawa
Ken
K
Yasunaga
Teruo
T
Koyama
Koji
K
D002874:Chromosome Mapping; D041322:Chromosomes, Human, Y / Q000235:genetics*; D030541:Databases, Genetic; D000075364:Deleted in Azoospermia 1 Protein; D015870:Gene Expression; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000235:genetics; D029589:Seminal Plasma Proteins / Q000235:genetics*
C466623:BPY2 protein, human; C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029589:Seminal Plasma Proteins; C480315:TTTY4 protein, human