Caenorhabditis elegans dna-2 is involved in DNA repair and is essential for germ-line development.
Caenorhabditis elegans germ cell proliferation and development were severely damaged in second generation dna-2 homozygotes. Even in the first generation, a much higher incidence of aberrant chromosomes in oocytes and resultantly higher embryonic lethality were found vs. wild type, when DNA breaks were induced by gamma-rays or camptothecin. The deficiency of dna-2 in combination with RNA interference on mre-11 gene expression synergistically aggravated germ-line development, especially oocyte formation. These results suggest that C. elegans Dna-2 is involved in a DNA repair pathway paralleling homologous recombination or non-homologous end joining with mre-11 participation.
Lee
Myon Hee
MH
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, South Korea.
Han
Sung Min
SM
Han
Ji Won
JW
Kim
Yun Mi
YM
Ahnn
Joohong
J
Koo
Hyeon-Sook
HS
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D002166:Camptothecin / Q000494:pharmacology; D002869:Chromosome Aberrations; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair; D018509:DNA, Helminth / Q000502:physiology*; D005720:Gamma Rays; D005804:Genes, Lethal; D005854:Germ Cells / Q000378:metabolism; D006579:Heterozygote; D006720:Homozygote; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010063:Ovum / Q000502:physiology / Q000528:radiation effects; D010641:Phenotype; D034622:RNA Interference; D013696:Temperature
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; C423224:mre-11 protein, C elegans; D002166:Camptothecin