title: Respiratory chlamydial infection based on experimental aerosol challenge of pigs with Chlamydia suis.
abstract: An experimental study of aerogeneous challenge in pigs was conducted in order to reveal characteristic features of porcine respiratory chlamydiosis. Eight conventionally raised pigs were exposed to a pathogenic strain of Chlamydia (C.) suis, four controls were mock infected. Besides pathological changes, the acute-phase and humoral immune responses, as well as the dissemination and transmission of the challenge strain was monitored in the course of infection. The data from clinical investigations, LPS-binding protein assay, antibody ELISAs, confocal laser scanning and light microscopy, immunohistochemical staining and PCR provided extensive evidence of the pathogenic potential of C. suis for the porcine respiratory system. This model appears suitable for further pathophysiological and immunological investigations of chlamydial respiratory infections and can also be recommended for studies of Chlamydia-associated infections of the human lung.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals (BFAU), Jena Institutes, Naumburger Str. 96a, 07743, Jena, Germany. k.sachse@jena.bfav.de
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Ernst
authors.initials: E
authors.lastname: Grossmann
authors.forename: Angela
authors.initials: A
authors.lastname: Berndt
authors.forename: Christine
authors.initials: C
authors.lastname: Schütt
authors.forename: Klaus
authors.initials: K
authors.lastname: Henning
authors.forename: Dirk
authors.initials: D
authors.lastname: Theegarten
authors.forename: Olaf
authors.initials: O
authors.lastname: Anhenn
authors.forename: Petra
authors.initials: P
authors.lastname: Reinhold
mesh_terms: D000209:Acute-Phase Proteins; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001831:Body Temperature; D002352:Carrier Proteins / Q000097:blood; D018834:Chaperonin 60 / Q000097:blood; D002689:Chlamydia / Q000235:genetics / Q000254:growth & development*; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008562:Membrane Glycoproteins; D018613:Microscopy, Confocal / Q000662:veterinary; D014066:Palatine Tonsil / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012141:Respiratory Tract Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein