title: Selective staining by vital dyes of Müller glial cells in retinal wholemounts.
abstract: Müller glial cells within the retina may respond to different signaling molecules with an elevation of their intracellular free calcium. To prove the localization of the recorded calcium responses in Müller cells within acutely isolated retinal wholemounts, retinal pieces from adult animals and humans were exposed to different vital dyes just after the calcium imaging records were finished. The dyes, Mitotracker Orange, Mitotracker Green, Celltracker Orange, Celltracker Green, and monochlorobimane, are all selectively taken up by Müller glial cells, while neuronal cells remain largely devoid of the dyes. By using this method, it can be demonstrated that the free calcium alterations within the wholemounts indeed occur within Müller cells. Moreover, the cross-sectional areas of (dye-filled) Müller glial cell bodies, as well as of (dye-free) neuronal cell bodies, can be measured in retinal wholemounts, and the spatial densities of both types of cells can be determined. The vital dye loading of Müller cells may facilitate investigations of stimulus-induced alterations of retinal glial cell physiology and morphology.
authors.affiliation: Paul Flechsig Institute of Brain Research, Department of Neurophysiology, University of Leipzig, Leipzig, Germany.
authors.forename: Ortrud
authors.initials: O
authors.lastname: Uckermann
authors.forename: Ianors
authors.initials: I
authors.lastname: Iandiev
authors.forename: Mike
authors.initials: M
authors.lastname: Francke
authors.forename: Kristian
authors.initials: K
authors.lastname: Franze
authors.forename: Jens
authors.initials: J
authors.lastname: Grosche
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wolf
authors.forename: Leon
authors.initials: L
authors.lastname: Kohen
authors.forename: Peter
authors.initials: P
authors.lastname: Wiedemann
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.forename: Andreas
authors.initials: A
authors.lastname: Bringmann
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000032:analysis*; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D009457:Neuroglia / Q000737:chemistry* / Q000166:cytology; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000737:chemistry* / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents