title: Chromosomal aberrations and micronuclei in lymphocytes of breast cancer patients after an accident during radiotherapy with 8 MeV electrons.
abstract: In February 2001 a radiation accident occurred in a radiotherapy unit of an oncology hospital in Poland. Five breast cancer patients undergoing radiotherapy received a single high dose of 8 MeV electrons. The exact doses are not known, but they were heterogeneous and may have reached about 100 Gy. To assess whether such exposure would be detectable in peripheral blood lymphocytes, chromosomal aberrations and micronuclei were analyzed in lymphocytes from the accident patients and compared to values for lymphocytes from 10 control patients who were not involved in the accident but who received similar radiotherapy treatments. Lymphocytes were harvested for analysis of chromosomal aberrations at three different culture times to determine whether heavily damaged cells reached mitosis with a delay. There was no effect of harvest time on the frequencies of chromosomal aberrations, indicating that there was no delay of heavily damaged cells in entering mitosis. A good correlation was observed between micronuclei and chromosomal aberrations. In lymphocytes from three of the accident patients, significantly enhanced frequencies of both aberrations and micronuclei were found. The great individual variability observed in the frequency of cytogenetic damage in lymphocytes from both control and accident patients precluded the unambiguous identification of all accident patients.
authors.affiliation: Department of Radiation Biology and Health Protection, Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warszawa, Poland. awojcik@ichtj.waw.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojcik
authors.forename: Günther
authors.initials: G
authors.lastname: Stephan
authors.forename: Sylwester
authors.initials: S
authors.lastname: Sommer
authors.forename: Iwona
authors.initials: I
authors.lastname: Buraczewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kuszewski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wieczorek
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Gózdz
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics / Q000532:radiotherapy*; D002869:Chromosome Aberrations; D004583:Electrons / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D048629:Micronuclei, Chromosome-Defective; D008875:Middle Aged; D018788:Radioactive Hazard Release