title: Ethylene stimulates endoreduplication but inhibits cytokinesis in cucumber hypocotyl epidermis.
abstract: The effects of ethylene on cell division are generally considered inhibitory. In this study, we demonstrate that transient ethylene exposure, while suppressing cytokinesis, stimulates DNA synthesis. We monitored DNA synthesis and cytokinesis in the epidermis of cucumber (Cucumis sativus) hypocotyls, an organ whose post-germination development involves strictly limited cell division. During exposure to ethylene, DNA synthesis, assessed by the incorporation of the thymidine homolog 5-bromo-2'-deoxyuridine, was detected in 20% of the epidermal cells, whereas DNA synthesis was nearly undetectable in normal air. Cytofluorometric analysis of nuclei in affected cells showed an up to 8-fold increase in DNA content. During this time, new cell plate formation was not detected. However, shortly after ethylene was removed, DNA content was rapidly restored to 2C (diploid) levels in all cells, and new cell plate formation dramatically increased. These results demonstrate that ethylene promotes DNA synthesis and its endoreduplication but inhibits cytokinesis, thereby maintaining some cells in G2 phase.
authors.affiliation: Department of Biology, International Christian University, Mitaka, Tokyo 181-8585 Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Dan
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Imaseki
authors.forename: Geoffrey O
authors.initials: GO
authors.lastname: Wasteneys
authors.forename: Haruko
authors.initials: H
authors.lastname: Kazama
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D018553:Cucumis sativus / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D004261:DNA Replication / Q000187:drug effects; D018744:DNA, Plant / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology*; D018546:Hypocotyl / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D007700:Kinetics; D010937:Plant Growth Regulators / Q000494:pharmacology*
chemical_list: D018744:DNA, Plant; D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene