title: Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.
abstract: OBJECTIVE
  To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.
  
  
  METHODS
  The whole-cell patch clamp technique was used.
  
  
  RESULTS
  Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms.
  
  
  CONCLUSIONS
  Pae inhibited I(Na) in mouse hippocampus neurons.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Guang-Qin
authors.initials: GQ
authors.lastname: Zhang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Hao
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Chen
authors.forename: Pei-Ai
authors.initials: PA
authors.lastname: Zhou
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Cheng
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Wu
mesh_terms: D000818:Animals; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D039821:Monoterpenes; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D015222:Sodium Channels / Q000378:metabolism*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; D026941:Sodium Channel Blockers; D015222:Sodium Channels; C015423:peoniflorin; D006027:Glycosides