title: Effect of extraction method on the concentrations of selected bioactive compounds in mandarin juice.
abstract: A mandarin-type citrus fruit, ponkan (Citrus reticulata), was processed by in-line, chopper pulper, and hand-press extractions to investigate the effect of extraction method on the concentrations of bioactive compounds in processed juice. Concentrations of polymethoxylated flavones (tangeretin, nobiletin, and sinensetin) and beta-cryptoxanthin in juice, and inhibitory activities against arachidonate cyclooxygenase and lipoxygenases of the juice extract were analyzed. The juice processed by hand-press extraction contained the largest amounts of nobiletin (3.56 mg/100 mL), tangeretin (4.10 mg/100 mL), and sinensetin (0.13 mg/100 mL). Concentrations of beta-cryptoxanthin were 0.66, 0.59, 0.55, and 0.50 mg/100 mL in chopper pulper, in-line (5/64 in.), in-line (8/64 in.) and hand-press juices, respectively. Both extracts of in-line juices showed greater inhibitory activity toward platelet 12-lipoxygenase than the others. The inhibitory effect of hand-press juice extract on platelet cyclooxygenase activity was remarkable among juice extracts. All juice extracts effectively inhibited polymorphonuclear 5-lipoxygenase activity at nearly the same rate.
authors.affiliation: National Agricultural Research Center for Western Region, Fukuyama, Hiroshima 721-8514, Japan. ynogata@affrc.go.jp
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nogata
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ohta
authors.forename: Takashi
authors.initials: T
authors.lastname: Sumida
authors.forename: Keizo
authors.initials: K
authors.lastname: Sekiya
mesh_terms: D001628:Beverages / Q000032:analysis*; D002957:Citrus / Q000737:chemistry*; D065366:Cryptoxanthins; D016861:Cyclooxygenase Inhibitors / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D016859:Lipoxygenase Inhibitors / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D065366:Cryptoxanthins; D016861:Cyclooxygenase Inhibitors; D005419:Flavonoids; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D024341:Xanthophylls; D019207:beta Carotene