title: [Study on mechanical compression regulating the proliferation of young growing rat masticatory myocyte in vitro].
abstract: OBJECTIVE
  To establish an experimental model which was the masticatory myocyte culture and force stimulation in vitro and study the proliferation changes of cultured masticatory myocytes caused by mechanical compression in young rat.
  
  
  METHODS
  Flow cytometry (FCM) was used to examine the changes of cellular DNA content and cell cycles of cultured masticatory myocytes.
  
  
  RESULTS
  The DNA content in experimental groups was higher than that in control. After the compressive force was applied for 2 hours, the proliferation index (PI) in experimental group became higher than that in control. Under a continuous pressure for 4 hours, the PI in 2,000 mu strain group reached the maximum (48.9%) but the PI in 4,000 mu strain group reached the minimum (39.0%).
  
  
  CONCLUSIONS
  The proliferation of masticatory myocytes from young rat increased under certain force and certain period of time, but decreased if the force applied was overloaded.
authors.affiliation: Department of Orthodontics, Oral Hospital of Guangdong Province, Guangzhou 510280, China.
authors.forename: Kaiyun
authors.initials: K
authors.lastname: Chen
authors.forename: Songjiao
authors.initials: S
authors.lastname: Luo
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D003799:Dental Stress Analysis; D005434:Flow Cytometry; D008410:Masticatory Muscles / Q000166:cytology* / Q000502:physiology; D032342:Muscle Cells / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical
chemical_list: D004247:DNA