title: Characterization of plasma membrane respiratory chain and ATPase in the actinomycete Nonomuraea sp. ATCC 39727.
abstract: We have characterized the respiratory system of the aerobic actinomycete Nonomuraea sp. ATCC 39727. The plasma membrane of the microorganism is shown to contain a protonmotive respiratory chain and H+-ATPase. The respiratory chain is made up of a rotenone-sensitive NADH-quinone oxidoreductase, a four subunits aa3-type cytochrome c oxidase and a bc1 complex. The H+-ATPase is characterized as an F0F1-type on the basis of its sensitivity to specific inhibitors; the enzyme is also inhibited by mM concentrations of Ca2+. The activity of the respiratory chain increases during the exponential growth phase, but is depressed in the stationary phase. The H+-ATPase activity reaches, as the respiratory chain, a maximal activity at the end of the exponential growth phase and then remains constant in the stationary phase.
authors.affiliation: Department of Medical Biochemistry and Biology, University of Bari, Bari, Italy.
authors.forename: L L
authors.initials: LL
authors.lastname: Palese
authors.forename: A
authors.initials: A
authors.lastname: Gaballo
authors.forename: Z
authors.initials: Z
authors.lastname: Technikova-Dobrova
authors.forename: N
authors.initials: N
authors.lastname: Labonia
authors.forename: A
authors.initials: A
authors.lastname: Abbrescia
authors.forename: S
authors.initials: S
authors.lastname: Scacco
authors.forename: L
authors.initials: L
authors.lastname: Micelli
authors.forename: S
authors.initials: S
authors.lastname: Papa
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D025243:Bacterial Proton-Translocating ATPases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D012402:Rotenone / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003486:Cyanides; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D014475:Uncoupling Agents; D012402:Rotenone; D000968:Antimycin A; D003576:Electron Transport Complex IV; D025243:Bacterial Proton-Translocating ATPases; D042967:Electron Transport Complex I; D002118:Calcium