Simple setup for nanosecond time-resolved spectroscopic measurements by a digital storage oscilloscope.
An application of a digital storage oscilloscope for nanosecond time-resolved spectroscopic measurements is demonstrated in the range from the single-photon region to the multi-photon region. In comparison to the time-correlated single photon counting (TCSPC) method, the measurement setup can be greatly simplified by averaging the signals measured by the oscilloscope. Moreover, the multi-photon events of the fluorescence emissions can be tracked by this simple setup although there still exist some disadvantages in the dynamic range of the signal due to radio frequency noise, and the temporal response of the photo-multiplier tube. This method can simplify time-resolved optical measurements in the nanosecond range, such as fluorescence decay and time-of-flight measurements of diffusing light. Thus, this simple method will be applicable in many clinical and industrial uses.
Nishimura
Goro
G
Biophysics Laboratory, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan. gnishi@imd.es.hokudai.ac.jp
Tamura
Mamoru
M
D000703:Analog-Digital Conversion; D003626:Data Display; D019544:Equipment Failure Analysis; D016247:Information Storage and Retrieval / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D020112:Rhodamine 123 / Q000032:analysis*; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors
D020112:Rhodamine 123