Inhibition of staurosporine-induced neuronal cell death by bisphenol A and nonylphenol in primary cultured rat hippocampal and cortical neurons.
We examined whether bisphenol A (BPA) and 4-nonylphenol (NP) influenced staurosporine-induced neuronal cell death in primary cultured rat hippocampal and cortical neurons. In hippocampal neurons, 17beta-estradiol (E2) (1 nM and 10 microM) and BPA (10 microM) significantly inhibited the staurosporine-induced release of lactate dehydrogenase (LDH). In cortical neurons, BPA significantly inhibited the LDH release, while E2 did not. In hippocampal neurons, E2 and BPA significantly inhibited the staurosporine-induced increase in caspase-3 activity. In cortical neurons, BPA and NP significantly inhibited the increase in caspase-3 activity, while E2 did not. Furthermore, low-dose BPA (10 nM) also significantly inhibited the increase in caspase-3 activity in both hippocampal and cortical neurons. BPA and NP might impede normal brain development by inhibiting even desirable neuronal cell death, interfering with caspase-3 activation.
Negishi
Takayuki
T
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. taka-u@yayoi.club.ne.jp
Ishii
Yoshiyuki
Y
Kyuwa
Shigeru
S
Kuroda
Yoichiro
Y
Yoshikawa
Yasuhiro
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000633:toxicity; D004958:Estradiol / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019311:Staurosporine / Q000633:toxicity; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D053148:Caspase 3; D020169:Caspases; D004791:Enzyme Inhibitors; D004958:Estradiol; D016166:Free Radical Scavengers; D007770:L-Lactate Dehydrogenase; D010636:Phenols; D019311:Staurosporine; C025256:nonylphenol; C505410:Casp3 protein, rat; C006780:bisphenol A; D000098826:Bisphenol A Compounds