title: Combination effects of complement regulatory proteins and anti-complement polymer.
abstract: We previously reported the development of a "cytomedicine" that consists of cells trapped in alginate-poly-L-lysine-alginate (APA) microcapsules and agarose microbeads. The functional cells that are entrapped in semipermeable polymer are completely isolated from cellular immune system. However, the ability of cytomedicine to isolate cells from the humoral immune system, which plays an essential role in xenograft rejection, is low. Therefore, the goal of the present study was to develop a novel cytomedicine that could protect the entrapped cells from injury of the complement system. We investigated the applicability of the complement regulatory protein (CRP), Crry, to cytomedicine. Crry-transfected cells entrapped within agarose microbeads resisted injury by complement to a degree, while entrapment of Crry transfected cells within agarose microbeads containing polyvinyl sulfate (PVS), a novel cytomedical device with anti-complement activity, clearly protected against complement attack. These data indicate that the combination of a CRP and a cytomedical device with anti-complement activity is a superior device for cytomedical therapy.
authors.affiliation: Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Okamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Okada
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mukai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shibata
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Natsuki
authors.initials: N
authors.lastname: Dohi
authors.forename: Noriko
authors.initials: N
authors.lastname: Okada
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Tadanori
authors.initials: T
authors.lastname: Mayumi
mesh_terms: D000818:Animals; D016466:CHO Cells; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D006224:Cricetinae; D007165:Immunosuppression Therapy; D011145:Polyvinyls / Q000494:pharmacology*; D011951:Receptors, Complement / Q000235:genetics*; D012685:Sepharose / Q000494:pharmacology*; D014162:Transfection
chemical_list: D003169:Complement Inactivator Proteins; D011145:Polyvinyls; D011951:Receptors, Complement; C019050:polyvinyl sulfate; D012685:Sepharose