EROD activity in gill filaments of anadromous and marine fish as a biomarker of dioxin-like pollutants.
The applicability of a gill filament-based ethoxyresorufin O-deethylase (EROD) assay, originally developed in rainbow trout, was examined in Atlantic salmon (Salmo salar), Arctic charr (Salvelinus alpinus), Atlantic cod (Gadus morhua), saithe (Pollachius virens) and spotted wolffish (Anarhichas minor). All species but spotted wolffish showed strong EROD induction in tip pieces of gill filaments following 48 h of exposure to waterborne beta-naphthoflavone. Atlantic salmon parr, smolts held in freshwater and smolts transferred to seawater showed EROD induction of similar magnitude. Arctic charr, differing 11-fold in body weight, showed similar EROD activities as expressed per gill filament tip. Laboratory exposure of saithe to water and sediments collected at polluted sites, resulted in strong EROD induction. In conclusion, the gill filament assay seems useful for monitoring exposure to aryl hydrocarbon receptor agonists in various species. Furthermore, smoltification status, water salinity and body size proved to have minor influence on gill filament EROD activity. However, the results in spotted wolffish show that some species may be less suitable for monitoring using the gill assay. Assessment of gill filament EROD activity in fish exposed to polluted water and sediments in the laboratory proved to be an easy and cost-effective way to survey pollution with dioxin-like chemicals.
Jönsson
Maria
M
Department of Environmental Toxicology, Centre for Reproductive Biology in Uppsala, Uppsala University, Sweden.
Abrahamson
Alexandra
A
Brunström
Björn
B
Brandt
Ingvar
I
Ingebrigtsen
Kristian
K
Jørgensen
Even H
EH
D000818:Animals; D001681:Biological Assay; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004147:Dioxins / Q000032:analysis* / Q000633:toxicity; D004784:Environmental Monitoring; D005399:Fishes / Q000254:growth & development; D005880:Gills / Q000187:drug effects / Q000201:enzymology*; D008018:Life Cycle Stages; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
D015415:Biomarkers; D004147:Dioxins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1