Preferential orientation of an immunoglobulin in a glycolipid monolayer controlled by the disintegration kinetics of proteo-lipidic vesicles spread at an air-buffer interface.
The insertion of immunoglobulin (IgG) in a glycolipid monolayer was achieved by using the ability of new proteo-glycolipid vesicles to disintegrate into a mixed IgG-glycolipid interfacial film after spreading at an air-buffer interface. The interfacial disintegration kinetics was shown to be directly dependent on the initial vesicle surface density and on the buffer ionic strength. The presence of the immunoglobulin in the glycolipid film was displayed by an increase of the lateral compressibility (Cs) during monolayer compression. Cs magnitude modifications, due to the antibody effect on the monolayer packing, decreases as the spread vesicle density increases. At interfacial saturation, the lateral compressibility profile becomes similar to that of a control monolayer without antibody. However, the careful analysis of the mixed monolayer after transfer by Langmuir-Blodgett technique (ATR-FTIR characterisation, enzyme immunoassociation) clearly demonstrated that the antibody was still present in such conditions and was not completely squeezed out from the interface as compressibility changes could have meant. At nonsaturating vesicle surface density, IgG molecules initially lying in the lipid matrix with the Y-shape plane parallel to the interface move to a standing-up position during the compression, leading to lateral compressibility modifications. For a saturating vesicle surface density, the glycolipid molecules force the IgG molecules to directly adopt a more vertical position in the interfacial film and, consequently, no lateral compressibility modification was recorded during the compression.
Girard-Egrot
Agnès P
AP
UMR 5013/EMB2-CNRS/UCBL, Université Claude Bernard Lyon 1, 43 Bvd du 11 novembre 1918, cedex F-69622 Villeurbanne, France. agnes.ergot@univ-lyon.fr
Godoy
Stéphanie
S
Chauvet
Jean Paul
JP
Boullanger
Paul
P
Coulet
Pierre R
PR
D000388:Air; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D015374:Biosensing Techniques; D002021:Buffers; D004058:Diffusion; D006017:Glycolipids / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D007700:Kinetics; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry*; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D011487:Protein Conformation; D012996:Solutions; D013499:Surface Properties; D013500:Surface Tension
D001672:Biocompatible Materials; D002021:Buffers; D006017:Glycolipids; D007074:Immunoglobulin G; D008081:Liposomes; D008567:Membranes, Artificial; D012996:Solutions