Effect of plasma from patients with Behçet's disease on the production of nitric oxide in cultured human umbilical vein endothelial cells.
OBJECTIVE
To determine the effect of plasma from patients with Behçet's disease (BD) on the production of nitric oxide (NO) in cultured human umbilical vein endothelial cells (HUVECs).


METHODS
NO levels were measured in cell culture media after 24-hour incubation of the cells with plasma obtained from 22 BD patients and 16 age/sex-matched healthy control subjects. After treatment of the patients with colchicine and/or nonsteroidal anti-inflammatory drugs, 12 of the patients were considered to be in the inactive phase of the disease. Levels of NO production were also measured in these 12 patients. In addition to the in vitro experiments, erythrocyte sedimentation rate, alpha(1)-antitrypsin, alpha(2)-macroglobulin and neutrophil counts were measured in the patients and controls.


RESULTS
Levels of NO in active state BD patients (15.9 micromol/10(6) cells) were significantly lower than values obtained from both patients in the inactive period (19.2 micromol/10(6) cells) and the control group (19.7 micromol/10(6) cells). No significant differences were observed in induced NO products between the patients in the inactive stage and control subjects.


CONCLUSIONS
Plasma from BD patients decreased the level of NO production in the HUVECs, and therefore may cause dysfunction in the endothelial NO synthase activity.
Orem
Asim
A
Department of Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. aorem64@yahoo.com
Ertürk
Murat
M
Cimşit
Gülseren
G
Kural
Birgül Vanizor
BV
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001528:Behcet Syndrome / Q000097:blood* / Q000188:drug therapy; D002478:Cells, Cultured; D003078:Colchicine / Q000627:therapeutic use; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000097:blood; D010949:Plasma; D014471:Umbilical Veins / Q000166:cytology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009569:Nitric Oxide; D003078:Colchicine