Colorimetric in vitro evaluation of T-cell cytotoxicity.
We propose a new colorimetric method for evaluation of specific T lymphocyte cytotoxicity based on the capacity of viable cells to incorporate alamar blue dye, an indicator of cell metabolic activity. This method is effective for highly specific strains of cytotoxic T lymphocytes and for primary T killers formed in vivo and constituting a small part of analyzed cell population. This test is a simple nonradioactive method for evaluating specific cellular cytotoxicity exhibiting sufficient sensitivity for evaluation of this parameter in the presence of effector cells.
Khromykh
L M
LM
N. N. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Anfalova
T V
TV
Kazanskii
D B
DB
D000818:Animals; D002461:Cell Line, Transformed; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D004396:Coloring Agents / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010078:Oxazines / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism; D013997:Time Factors; D014966:Xanthenes / Q000494:pharmacology
D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin