title: Identification of a human erythroid progenitor cell population which expresses the CD34 antigen and binds the plant lectin Ulex europaeus I.
abstract: Two and three color flow cytometry of normal human bone marrow was used to identify CD34+ progenitor cells and examine their binding to the plant lectin Ulex europaeus I (Ulex). In normal bone marrow, 48.48 +/- 17.4% of the CD34+ cells bind to Ulex. Two color flow cytometry was used to sort CD34 + cells, and subsets of CD34+ cells, CD34+ Ulex+ and CD34+ Ulex-. These populations were sorted into colony assays to assess myeloid (CFU-GM) and erythroid (BFU-E) progenitors. The CD34+ Ulex+ subset was 84 +/- 14% BFU-E colonies (mean +/- S.D.) and had the highest cloning efficiency of 28 +/- 13%. Three color analysis of CD34+ Ulex+ cells showed staining with other erythroid (CD71, GlyA) antibodies and lack of stain. ing with myeloid (CD13, CD45RA) antibodies. These studies confirmed the erythroid characteristics of this subpopulation.
authors.affiliation: Immunotherapy Division, Biotech Group, Baxter Healthcare Corporation, Round Lake Illinois 60073, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Unverzagt
authors.forename: J
authors.initials: J
authors.lastname: Martinson
authors.forename: W
authors.initials: W
authors.lastname: Lee
authors.forename: P J
authors.initials: PJ
authors.lastname: Stiff
authors.forename: S
authors.initials: S
authors.lastname: Williams
authors.forename: J G
authors.initials: JG
authors.lastname: Bender
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D018952:Antigens, CD34 / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D019070:Cell Lineage / Q000276:immunology*; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D018952:Antigens, CD34; D015415:Biomarkers; D037121:Plant Lectins; C036850:Ulex europaeus lectins