title: Cloning and characterization of a novel Hsp100/Clp gene (osClpD) from Oryza sativa.
abstract: A novel osClpD gene, encoding a highly conservative ClpD subfamily member, was first isolated and characterized from Oryza sativa. The full-length cDNA of osClpD gene was 3140 bp and contained a 2884 bp open reading frame encoding a 938 amino acid protein. The phylogenetic tree and blast search showed that OSClpD belonged to the ClpD subfamily of the Hsp100/Clp family, and contained all protein motifs characteristic for the ClpD subfamily of Hsp100/Clp proteins. The real-time quantitative PCR analysis proved that it was inducible by water deficit and temperature stress in vegetative tissues.
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Shen
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Pang
authors.forename: Chang-Fa
authors.initials: CF
authors.lastname: Lin
authors.forename: Chun
authors.initials: C
authors.lastname: Wei
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Qian
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Jiang
authors.forename: Xi-Ling
authors.initials: XL
authors.lastname: Du
authors.forename: Ke-Gui
authors.initials: KG
authors.lastname: Li
authors.forename: Kotb
authors.initials: K
authors.lastname: Attia
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: Yang
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003890:Desiccation; D049071:Endopeptidase Clp; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics*; D013696:Temperature
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases