title: Altered responses of chondrocytes to nanophase PLGA/nanophase titania composites.
abstract: Chondrocyte (cartilage-synthesizing cells) cell density and synthesis of select intracellular proteins by chondrocytes were investigated on novel nanophase poly-lactic/glycolic acid (PLGA) and titania composites in the present in vitro study. Nanophase PLGA films were created by chemically treating conventional (or micron-structured) PLGA films with 10N NaOH for 1h. Titania particle dimensions in ceramic compacts were controlled by utilizing either conventional (i.e., micron) or nanometer grain size titania. Composites of either conventional or nanophase PLGA with either conventional or nanophase titania at 70/30wt% were also created. Compared to surfaces with a conventional or micron topography, results provided the first evidence of stagnant confluent cell densities on nanostructured surfaces at time points between 1 and 7 days. Moreover, compared to surfaces with a conventional topography, increased chondrocyte intracellular synthesis of alkaline phosphatase and chondrocyte expressed protein-68 (proteins that have been correlated with the functions of chondrocytes) were observed on nanophase PLGA/nanophase titania composites. The present study, thus, provided the first evidence of different chondrocyte responses to nanostructured PLGA/nanophase titania composites; in light of other reports demonstrating increased functions of bone cells on the same materials, such data indicates that further investigation of these materials at the bone-cartilage interface should be conducted.
authors.affiliation: Department of Biomedical Engineering, Purdue University, 1296 Potter Eng. Building, West Lafayette, IN 47907-1296, USA.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Savaiano
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008420:Manufactured Materials; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D044367:Phase Transition; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C422245:CRTAC1 protein, human; D002135:Calcium-Binding Proteins; D011108:Polymers; D011506:Proteins; C009495:titanium dioxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014025:Titanium