title: Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples.
abstract: Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized. Both conjugate-coated ELISAs are based on monoclonal antibodies (MAbs) of different specificity and homologous protein conjugates. Effects of several physicochemical factors (ionic strength, pH, Tween-20 and Bovine serum albumin (BSA) concentrations) and solvents (methanol, ethanol, acetone and N,N'-dimethylformamide) on the performance of the assays were studied and optimized. For the DDT-selective assay, the sensitivity, estimated as the I(50) value, was 0.6 microg/l, with a linear working range between 0.1 and 2 microg/l and a limit of detection of 0.06 microg/l. For the DDT group-selective assay, the sensitivity was 0.2 microg/l, with a linear working range between 0.07 and 1 microg/l and a limit of detection of 0.04 microg/l. CL-ELISAs were four times more sensitive compared to colorimetric ELISAs. Finally, both immunoassays were applied to the detection of DDT and group of DDT-related compounds in spiked real water, soil and food samples.
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119899 Moscow, Russia.
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Botchkareva
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.forename: Angel
authors.initials: A
authors.lastname: Montoya
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Manclús
authors.forename: Barbora
authors.initials: B
authors.lastname: Mickova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rauch
authors.forename: Fabiana
authors.initials: F
authors.lastname: Fini
authors.forename: Stefano
authors.initials: S
authors.lastname: Girotti
mesh_terms: D003429:Cross Reactions; D003634:DDT / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D012987:Soil / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D012987:Soil; D014867:Water; D003634:DDT