[Influence of different temperatures on internalization of epidermal growth factor receptor complexes in Tca-8113 cells].
OBJECTIVE
To investigate the effect of different temperatures on internalization of epidermal growth factor receptor complexes in human tongue squamous carcinoma cells(Tca-8113).


METHODS
Human epidermal growth factor r (hEGF) was labeled with 125I according to Chloramine-T method. The quantity of surface binding and internalization of 125I-hEGF in Tca-8113 cells at 4 degrees C and at 37 degrees C was compared respectively with radiobinding assay.


RESULTS
At 37 degrees C, the amount of EGFR complexes internalization exceeded that of surface binding after 10 min. At 4 degrees C, the level of interlization maintained very low.


CONCLUSIONS
The study indicated that the surface binding of 125I-hEGF at 4 degrees C was steadier than that at 37 degrees C. The study on phenomenon of EGFR complexes internalization provide the experimental basis for selecting anti-EGFR monoclonal antibody in clinic.
Sun
Mo-yi
MY
Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University, Xi'an 710032, China. moyisun@fmmu.edu.cn
Liu
Bao-lin
BL
Jing
Yue-gui
YG
Yang
Cheng
C
Shang
Hong-tao
HT
Li
Jian-hu
JH
D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D013696:Temperature
D007457:Iodine Radioisotopes; D004815:Epidermal Growth Factor; D066246:ErbB Receptors