[Characteristics of Bcl-2 and Bax expressions in rat brain after experimental intracerebral hemorrhage and intervention of naoyian granule].
OBJECTIVE
To investigate the characteristics of Bcl-2 and Bax expressions in rat brain after experimental intracerebral hemorrhagic (ICH), and to determine the protective mechanisms of the Traditional Chinese medicine complex, naoyian granule (NYA).


METHODS
ICH models were induced according to Rosenbergs method. The rats were divided into the model group, the NYA-treatment group and the sham group. Bcl-2 and Bax expressions in the rat brain after ICH were detected by immunohistochemistry method.


RESULTS
Bax expressed in the hemorrhagic core, and the immunoreactive neurons were seriously injuried and the shape varied. Bcl-2 expressed in the penumbra with the normal shape of the immunoreactive neurons. Both Bax and Bcl-2 expressed simultaneously in the marginal region of the cerebral hemorrhagic core. Bcl-2 expression in the rat brain reached the peak at the 2nd day and maintained at a relatively high level until the 7th day, and NYA could increase it. Bax expression peaked at the 1st day, and NYA could down-regulate it.


CONCLUSIONS
The expressions of Bcl-2 and Bax after the cerebral hemorrhage are related to the degree of injury, the time, and the location of the brain tissues. NYA can up-regulate the Bcl-2 expression and down-regulate the Bax expression, which may be one of its neuroprotective mechanisms.
Zhang
Hua-xian
HX
Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Li
Xing-qun
XQ
Tang
Tao
T
D000818:Animals; D001921:Brain / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein