title: Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells.
abstract: Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.
authors.affiliation: Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-8322, USA. jaeklee@virginia.edu
authors.forename: Jae K
authors.initials: JK
authors.lastname: Lee
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Bussey
authors.forename: Fuad G
authors.initials: FG
authors.lastname: Gwadry
authors.forename: William
authors.initials: W
authors.lastname: Reinhold
authors.forename: Gregory
authors.initials: G
authors.lastname: Riddick
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Pelletier
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishizuka
authors.forename: Gergely
authors.initials: G
authors.lastname: Szakacs
authors.forename: Jean-Phillipe
authors.initials: JP
authors.lastname: Annereau
authors.forename: Uma
authors.initials: U
authors.lastname: Shankavaram
authors.forename: Samir
authors.initials: S
authors.lastname: Lababidi
authors.forename: Lawrence H
authors.initials: LH
authors.lastname: Smith
authors.forename: Michael M
authors.initials: MM
authors.lastname: Gottesman
authors.forename: John N
authors.initials: JN
authors.lastname: Weinstein
mesh_terms: D000465:Algorithms; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*