title: Affinity purification of specific chromatin segments from chromosomal loci in yeast.
abstract: Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Joachim
authors.initials: J
authors.lastname: Griesenbeck
authors.forename: Hinrich
authors.initials: H
authors.lastname: Boeger
authors.forename: J Seth
authors.initials: JS
authors.lastname: Strattan
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D002843:Chromatin / Q000235:genetics* / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D015825:Chromosomes, Fungal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C051611:PHO2 protein, S cerevisiae; C060292:PHO4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors