title: Serial immunoprecipitation assays for interferon--(IFN)-beta antibodies in multiple sclerosis patients.
abstract: We devised a sensitive, radioimmunoprecipitation assay (RIPA) for anti-interferon (IFN)-beta-binding antibody (BAB) detection. Our RIPA showed good agreement with a reference RIPA (mean difference, -3.2 +/- 10.6 AU), and detected BAB to both IFN-beta-1a and IFN-beta-1b. Neutralizing antibodies to IFN-b (NAB) were also determined with a standard method. BAB and NAB were measured in 393 serum samples from 77 multiple sclerosis (MS) patients treated with IFN-beta-1a or -1b, who were studied over two years, and subsequently classified as responders and non-responders. BAB were found at higher concentrations, and more frequently detected, in IFN-beta-1b- than in IFN-beta-1a-treated patients, and, at highest titres, preferentially in patients who were positive for NAB. However, in our series of MS patients, both titres and frequency of detection of BAB or NAB did not differ between IFN-b responders and non-responders.
authors.affiliation: Department of Clinical Chemistry, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Lampasona
authors.forename: Jordi
authors.initials: J
authors.lastname: Rio
authors.forename: Diego
authors.initials: D
authors.lastname: Franciotta
authors.forename: Roberta
authors.initials: R
authors.lastname: Furlan
authors.forename: Carlo
authors.initials: C
authors.lastname: Avolio
authors.forename: Raffaella
authors.initials: R
authors.lastname: Fazio
authors.forename: Vito
authors.initials: V
authors.lastname: Lavolpe
authors.forename: Angela
authors.initials: A
authors.lastname: Vincent
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Comi
authors.forename: Maria
authors.initials: M
authors.lastname: Trojano
authors.forename: Xavier
authors.initials: X
authors.lastname: Montalban
authors.forename: Gianvito
authors.initials: G
authors.lastname: Martino
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D006801:Humans; D016899:Interferon-beta / Q000276:immunology*; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D016899:Interferon-beta