title: Nickel (Ni2+) enhancement of microtubule assembly in vitro is dependent on GTP function.
abstract: Microtubule (MT) assembly in vitro is accompanied by hydrolysis of tubulin-bound GTP at E-site. Ni2+, a human carcinogen, has been shown to markedly perturb the MT system in cultured cells and enhance MT assembly in vitro. To further probe the mechanisms of such multiple Ni2+ damaging actions on MT, we have focused on dissecting the role of the Ni2+/GTP interaction in influencing MT assembly in vitro as monitored by a turbidity assay at A350 at 27 degrees C using purified bovine brain MT proteins containing 162 microM each of Mg2+ and EGTA. MT assembly was initiated by addition of GTP and progressed in a GTP dose-dependent manner. The minimal and optimal exogenous [GTP] required for MT assembly were 15.6 and 500 microM, respectively. Replacement of GTP (25-87%) with increasing [NiCl2] while keeping the sum of [GTP] and [Ni2+] constant at 500 microM enabled MT assembly to proceed with shortened "lags" but reaching the same maximum plateau levels or elongation rates as with 500 microM GTP only. However, in reactions with Ni2+ replacing >94% of GTP, marked inhibition of MT assembly (lower plateaus) occurred. Electron microscopic (EM) examinations showed that MT formed with high Ni2+ substitutions for GTP appeared shorter, more numerous, and resistant to Ca2+ disruption than those assembled with 500 microM GTP only. Notably, in the presence of 500 microM Ni2+ with no GTP added, no typical MT were observed under EM, despite increases in turbidity of the reaction. In addition, the critical concentration of MT proteins required for assembly was also considerably decreased under conditions of Ni2+ replacements of GTP. These results point to an important role of GTP/Ni2+ interaction in modulating the Ni2+ enhancement of MT assembly in vitro.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Wande
authors.initials: W
authors.lastname: Li
authors.forename: Yinzhi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Donald L
authors.initials: DL
authors.lastname: Gantz
authors.forename: Iih-Nan
authors.initials: IN
authors.lastname: Chou
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D066298:In Vitro Techniques; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009391:Nephelometry and Turbidimetry; D009532:Nickel / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D004338:Drug Combinations; D008869:Microtubule-Associated Proteins; C022838:nickel chloride; D009532:Nickel; D006160:Guanosine Triphosphate