title: The response of the hepatic insulin-like growth factor system to growth hormone and dexamethasone in calves.
abstract: Glucocorticoids inhibit postnatal growth and yet can stimulate the somatotropic axis around birth. The aim of the present study was to investigate the effects of dexamethasone on the somatotropic axis and on the responses of the insulin-like growth factor (IGF) system to growth hormone treatment in calves. Calves (n=24) were randomly divided into four groups. Group DX was injected with dexamethasone (30 micro g/kg body weight per day), group GH was injected with 500 mg slow-release bovine growth hormone at 14-day intervals, group GHDX was injected with dexamethasone and bovine growth hormone, and group CNTRL (serving as control) was injected with saline from day 3 to day 42 of life. Blood samples were taken on day 3 and blood and liver samples were obtained on days 7, 14, 28 and 42. Body weight increased in the CNTRL and GH groups up to the end of the study and in the DX and GHDX groups up to the fourth week. Dexamethasone treatment decreased (P<0.05) plasma IGF binding protein (IGFBP)-1 on days 7 and 14, but increased (P<0.05) plasma IGFBP-1, decreased (P<0.05) plasma IGF-I and IGFBP-3, and decreased hepatic mRNA for growth hormone receptor (GHR) and IGF-I on day 42. Growth hormone treatment increased (P<0.05) plasma growth hormone concentrations on days 7 and 14, tended to increase (P<0.1) plasma IGF-I concentrations on day 42, and increased (P<0.05) hepatic mRNA levels of GHR on day 14 and IGF-I mRNA levels on days 7 and 14. The combined dexamethasone and growth hormone treatment increased plasma growth hormone concentrations on day 7 and resulted in the highest plasma concentrations of IGF-I and IGFBP-3 (day 7 to day 28) as well as the greatest abundance of hepatic GHR (day 14) and IGF-I (days 7 and 14) mRNA. Plasma IGFBP-1 concentrations in the GHDX group behaved in a similar manner as in the DX group. In conclusion, the response of the somatotropic axis to growth hormone treatment could be greatly enhanced by dexamethasone treatment during the neonatal and early postnatal period, but body weight gain was not improved. Dexamethasone alone inhibited the somatotropic axis and postnatal growth after the first Month of life.
authors.affiliation: Division of Animal Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, Bremgartenstr. 109a, CH-3012 Berne, Switzerland. harald.hammon@itz.unibe.ch
authors.forename: H M
authors.initials: HM
authors.lastname: Hammon
authors.forename: Y
authors.initials: Y
authors.lastname: Zbinden
authors.forename: H
authors.initials: H
authors.lastname: Sauerwein
authors.forename: B H
authors.initials: BH
authors.lastname: Breier
authors.forename: J W
authors.initials: JW
authors.lastname: Blum
authors.forename: S S
authors.initials: SS
authors.lastname: Donkin
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002417:Cattle / Q000254:growth & development / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004338:Drug Combinations; D004435:Eating; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006128:Growth / Q000187:drug effects; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000097:blood; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011986:Receptors, Somatotropin / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D004338:Drug Combinations; D005938:Glucocorticoids; D018970:Insulin-Like Growth Factor Binding Protein 1; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D011994:Recombinant Proteins; C073294:growth hormone, bovine; D007334:Insulin-Like Growth Factor I; D003907:Dexamethasone; D013006:Growth Hormone