Photodynamic effect of hypericin and a water-soluble derivative on isolated crayfish neuron and surrounding glial cells.
Hypericin (Hyp) has been proposed as a fluorochrome for fluorescence diagnostics and as a photosensitizer for photodynamic therapy of cancer. However, its insolubility in water is a serious drawback. A novel water-soluble hypericin derivative (Hyp-S) has been constructed, using polyvinylpyrrolidone as a carrier. We used the crayfish stretch receptor, consisting of receptor neuron and satellite glial cells, for comparison of the photodynamic effects of Hyp and Hyp-S. Hyp-S was more toxic in the dark than Hyp and inactivated the neurons at concentrations exceeding 4 microM while Hyp was toxic to the neurons only at the concentrations larger than 20 microM. Electrophysiological investigations revealed polyphasic neuron responses to photosensitization with Hyp as well as with Hyp-S (1 microM concentration, 30 min incubation; irradiation with filtered light from a lamp with an emission maximum near 600 nm and an intensity of 0.2 W/cm2). In the concentration range 1-4 microM Hyp-S was more phototoxic than Hyp. Fluorescence microscopy showed that both sensitizers were predominately localized in the glial envelope surrounding the neuron. A minor fraction of hypericin was found in the neuron perinuclear area rich in cytoplasm organelles. This suggests the potential application of Hyp and Hyp-S for visualization and selective photodynamic treatment of malignant gliomas.
Uzdensky
Anatoly B
AB
Rostov State University, Rostov-on-Don 344090, Russia. uzd@krinc.ru
Bragin
Denis E
DE
Kolosov
Mikhail S
MS
Kubin
Andreas
A
Loew
Hans G
HG
Moan
Johan
J
D000818:Animals; D000873:Anthracenes; D003400:Astacoidea / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D009457:Neuroglia / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility / Q000187:drug effects; D014867:Water / Q000737:chemistry / Q000494:pharmacology
D000873:Anthracenes; D017319:Photosensitizing Agents; D014867:Water; D010569:Perylene; C004965:hypericin