[Effects of Chinese herbal compound on the expression of hepatocyte cytochrome P450IIE1 in rats with alcoholic fatty liver].
OBJECTIVE
To study the effect of Chinese herbal compound (CHC) on the expression of hepatocyte cytochrome P450IIE1 in rat model of alcoholic fatty liver (AFL).


METHODS
The AFL rats models were established by administering the drinking water with 40%(v/v) ethanol, and the changes of pathology in liver and hepatocyte P450IIE1 expression, as well as the contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), vitamin E (VitE) in liver were detected and compared with those in the control group.


RESULTS
Fatty degeneration in liver recovered normally in the CHC-treated group. Immunohistochemical and in situ hybridization examination showed that CHC could inhibit the hepatocyte cytochrome P450IIE1 expression markedly, and restore the contents of MDA, SOD, GSH, VitE to nearly normal range.


CONCLUSIONS
CHC can prevent AFL through inhibiting the hepatocyte cytochrome P450IIE1 expression markedly
Dai
Ning
N
Department of Gastroenterology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China.
Zeng
Min-de
MD
Peng
Yan-shen
YS
Li
Ji-qiang
JQ
Qiu
De-kai
DK
Lu
Lun-gen
LG
D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005235:Fatty Liver, Alcoholic / Q000473:pathology*; D015870:Gene Expression; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D019392:Cytochrome P-450 CYP2E1