New insights on how nucleotide excision repair could remove DNA adducts induced by chemotherapeutic agents and psoralens plus UV-A (PUVA) in Escherichia coli cells.
Chemotherapeutic agents such as mitomycin C or nitrogen mustards induce DNA inter-strand cross-links (ICL) and are highly toxic, thus constituting an useful tool to treat some human degenerative diseases, such as cancer. Additionally, psoralens plus UV-A (PUVA), which also induce ICL, find use in treatment of patients afflicted with psoriasis and vitiligo. The repair of DNA ICL generated by different molecules involves a number of multi-step DNA repair pathways. In bacteria, as in eukaryotic cells, if DNA ICL are not tolerated or repaired via nucleotide excision repair (NER), homologous recombination or translesion synthesis pathways, these DNA lesions may lead to mutations and cell death. Herein, we bring new insights to the role of Escherichia coli nucleotide excision repair genes uvrA, uvrB and uvrC in the repair of DNA damage induced by some chemotherapeutic agents and psoralen derivatives plus UV-A. These new observations point to a novel role for the UvrB protein, independent of its previously described role in the Uvr(A)BC complex, which could be specific for repair of monoadducts, intra-strand biadducts and/or ICL.
Lage
Claudia
C
Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Bloco G, Centro de Ciencias da Saude, Universidade de Federal do Rio de Janeiro, 21949-900 Rio de Janeiro, RJ, Brazil.
de Pádula
Marcelo
M
de Alencar
Tatiana Amorim Muniz
TA
da Fonseca Gonçalves
Silvia Regina
SR
da Silva Vidal
Leonardo
L
Cabral-Neto
Januário
J
Leitão
Alvaro Costa
AC
D000970:Antineoplastic Agents / Q000494:pharmacology*; D018736:DNA Adducts / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D018736:DNA Adducts; D011564:Furocoumarins