title: A virulence attenuated amoebapore-less mutant of Entamoeba histolytica and its interaction with host cells.
abstract: Entamoeba histolytica, the protozoan parasite which causes amoebiasis, is an exclusively human pathogen so developing a vaccine could effectively impact the spread of the disease. Recently we developed a genetically modified avirulent strain, termed G3, from the virulent E. histolytica strain HM-1:IMSS. The new strain lacks the important virulence factor, the amoebapore-A. The objective of our current study was to investigate the avirulence of the attenuated strain as well as to examine the antigenic and immunogenic responses of these trophozoites as potential candidates for a live vaccine. Functional assays were conducted to characterise the virulent behaviour of the G3 strain. This behaviour was compared to the virulent strain HM-1:IMSS and the non-virulent strain Rahman. Western blots were conducted to confirm the lack of amoebapore-A in the E. histolytica G3 strain and to demonstrate that it had no influence on the presence of other virulence factors. Results of these two sets of tests proved the G3 strain to be phenotypically similar to the avirulent Rahman strain while antigenically identical to the virulent HM-1:IMSS, apart from the lack of the amoebapore-A protein. Intraperitoneal immunisation of hamsters with G3 trophozoites compared to sham immunised hamsters resulted in IgG anti-HM-1:IMSS antibodies. The level of humoral response was variable and further testing has to take place before introducing this new strain as a vaccine.
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel.
authors.forename: Shay
authors.initials: S
authors.lastname: Bujanover
authors.forename: Uriel
authors.initials: U
authors.lastname: Katz
authors.forename: Rivka
authors.initials: R
authors.lastname: Bracha
authors.forename: David
authors.initials: D
authors.lastname: Mirelman
mesh_terms: D000818:Animals; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000235:genetics* / Q000276:immunology; D004749:Entamoebiasis / Q000276:immunology; D020868:Gene Silencing; D017125:Genes, Protozoan; D006790:Host-Parasite Interactions; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D014613:Vaccines, Attenuated; D014774:Virulence
chemical_list: D007473:Ion Channels; D015800:Protozoan Proteins; D014613:Vaccines, Attenuated; C041670:amoebapore proteins, protozoan