Succinate dehydrogenase activity in rat dorsolateral ventral horn motoneurons at L6 after spaceflight and recovery.
Succinate dehydrogenase (SDH) activity levels of motoneurons in the rostral, middle, and caudal portions of the dorsolateral region of the ventral horn of the 6th lumbar (L6) segment of the rat spinal cord were determined after 14 days of spaceflight and after 9 days of recovery on Earth. The mean SDH activity of motoneurons with cell body sizes between 500 and 800 micrometers2 located in the rostral portion of the L6 segment was lower in spaceflight than in age-matched control rats. The decrease in motoneuron SDH activity persisted for at least 9 days of recovery on Earth. In contrast, the mean SDH activity of motoneurons located in the middle and caudal portions of the L6 segment were unaffected by spaceflight and recovery on Earth. The motoneurons in the rostral portion of the L6 segment presumably innervate both high- and low-oxidative fibers in hindlimb muscles, whereas those in the middle and caudal portions presumably innervate perineal muscles that are comprised only of low-oxidative fibers. These data indicate that moderate-sized motoneurons, most likely innervating fibers in high-oxidative muscles, are responsive to the microgravity environment.
Ishihara
Akihiko
A
Laboratory of Neurochemistry, Faculty of Integrated Human Studies, Kyoto University, Kyoto, Japan. ishihara@life.h.kyoto-u.ac.jp
Ohira
Yoshinobu
Y
Roy
Roland R
RR
Nagaoka
Shunji
S
Sekiguchi
Chiharu
C
Hinds
Willy E
WE
Edgerton
V Reggie
VR
D000818:Animals; D000870:Anterior Horn Cells / Q000166:cytology / Q000201:enzymology*; D048429:Cell Size; D006614:Hindlimb; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000201:enzymology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000201:enzymology; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology / Q000294:innervation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight; D013116:Spinal Cord / Q000166:cytology* / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014893:Weightlessness
D013385:Succinate Dehydrogenase