title: Attempts to inhibit ruminal methanogenesis by blocking pyruvate oxidative decarboxylation.
abstract: The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythiamin decreased methanogenesis by 23%. In the third experiment, three pyruvate derivatives did not inhibit methanogenesis, although hydroxypyruvate improved organic matter fermentation from 57.8% to 64.2%. The additives did not seem to inhibit pyruvate oxidative decarboxylation.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Ungerfeld
authors.forename: S R
authors.initials: SR
authors.lastname: Rust
authors.forename: R
authors.initials: R
authors.lastname: Burnett
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D003653:Decarboxylation; D005285:Fermentation; D007658:Ketone Oxidoreductases / Q000037:antagonists & inhibitors; D008697:Methane / Q000378:metabolism*; D010084:Oxidation-Reduction; D050743:Pyruvate Synthase; D019289:Pyruvic Acid / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012417:Rumen / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D013831:Thiamine / Q000378:metabolism
chemical_list: D003470:Culture Media; D019289:Pyruvic Acid; D007658:Ketone Oxidoreductases; D050743:Pyruvate Synthase; D008697:Methane; D013831:Thiamine