Intercellular bridges and apoptosis in clones of male germ cells.
When an As spermatogonium divides to form a pair of Apr spermatogonia the two daughter cells stay interconnected by an intercellular bridge. These cytoplasmic bridges form after every subsequent division leading to large clones of interconnected germ cells. Cohorts of spermatogonia maintain synchronous development throughout spermatogenesis, which has been attributed to the presence of these intercellular bridges. To examine whether apoptotic signals are transduced through the intercellular bridges we studied germ cell apoptosis in whole mounts of seminiferous tubules from non-irradiated and irradiated mouse testes, using whole mount seminiferous tubules and confocal microscopy. This allowed us to use TUNEL staining of apoptotic germ cells and at the same time to study these apoptotic germ cells in their topographical context. Our results show that in response to ionizing radiation single spermatogonia within a clone can undergo apoptosis without affecting their neighboring cells. Additionally, also early spermatocytes were shown to undergo apoptosis individually. Both radiation-induced spermatogonial apoptosis and spontaneous apoptosis of spermatocytes are caused by DNA damage of individual cells. Degeneration of healthy spermatogonia because of regulatory signals, however, follows other death inducing mechanisms, which lead to apoptosis of chains of interconnected spermatogonia.
Hamer
Geert
G
Department of Endocrinology, Faculty of Biology, Utrecht University, Utrecht, The Netherlands. g.hamer@bio.uu.nl
Roepers-Gajadien
Hermien L
HL
Gademan
Iris S
IS
Kal
Henk B
HB
De Rooij
Dirk G
DG
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002999:Clone Cells; D004307:Dose-Response Relationship, Radiation; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018613:Microscopy, Confocal; D011859:Radiography; D012671:Seminiferous Tubules / Q000000981:diagnostic imaging; D013093:Spermatogonia / Q000502:physiology / Q000528:radiation effects; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000000981:diagnostic imaging; D013997:Time Factors