Expression of IGF2 and IGF receptor mRNA in bovine nuclear transferred embryos.
Incomplete reprogramming of the donor cell nucleus after nuclear transfer (NT) probably leads to the abnormal expression of developmentally important genes. This may be responsible for the low efficiency of cloned animal production. Insulin-like growth factor 2 (IGF2) and IGF2 receptor (IGF2R) are imprinted genes that play important roles in preimplantation development. To obtain an insight into abnormal gene expression after nuclear transfer, we assessed the transcription patterns of IGF2-IGF2R in single in vitro fertilised and cloned embryos by reverse-transcription polymerase chain reaction (RT-PCR). IGF2R expression did not differ significantly but IGF2 was more highly expressed in cloned embryos than in IVF embryos (p < 0.05). This was confirmed by a quantitative RT-PCR method. Thus, incomplete reprogramming may induce abnormal transcription of IGF2 in cloned embryos.
Han
Dong-Wook
DW
Department of Animal Science, ARRC, Bio/Molecular Informatics Center, Konkuk University, Seoul, Korea.
Song
Sang-Jin
SJ
Uhum
Sang Jun
SJ
Do
Jeong-Tae
JT
Kim
Nam-Hyung
NH
Chung
Kil-Saeng
KS
Lee
Hoon Taek
HT
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000235:genetics*; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D017527:Receptor, IGF Type 2 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II