title: Polymerase chain reaction assay for Cydia pomonella granulovirus detection in Cydia pomonella population.
abstract: The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards,which had been infected with CpGV was tested during 2 years post infection. (p.i.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other hand, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population.
authors.affiliation: Research Institute of Crop Production, Drnovská 507, Prague 6, 161 06, Czech Republic. jiban@vurv.cz
authors.forename: J K
authors.initials: JK
authors.lastname: Kundu
authors.forename: J
authors.initials: J
authors.lastname: Stará
authors.forename: F
authors.initials: F
authors.lastname: Kocourek
authors.forename: O
authors.initials: O
authors.lastname: Pultar
mesh_terms: D000818:Animals; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D025722:Granulovirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D007814:Larva / Q000821:virology; D027845:Malus; D009036:Moths / Q000254:growth & development / Q000821:virology*; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements