Excised bone structures in mice: imaging at three-dimensional synchrotron radiation micro CT.
Bone microarchitecture and mineralization were determined at three-dimensional synchrotron radiation micro computed tomography in two inbred mice strains. Distal metaphysis of the left femur was imaged in three dimensions at 6.65 microm, whereas the right femur was analyzed with histomorphometry. Three-dimensional quantitative parameters of trabecular and cortical bone architecture were computed. C3H/HeJ@Ico mice had greater bone density and thicker trabeculae; greater cortical bone density, cortical thickness, and porosity; and greater mineralization than did C57BL/6J@Ico mice. The technique is well suited for assessment of trabecular and cortical bone in small animals and at the same time provides mineralization status in three dimensions.
Martín-Badosa
Estela
E
European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, France.
Amblard
Daniel
D
Nuzzo
Stefania
S
Elmoutaouakkil
Abdelmajid
A
Vico
Laurence
L
Peyrin
Françoise
F
D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000648:ultrastructure*; D002113:Calcification, Physiologic; D005269:Femur / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000379:methods*