Immunohistochemical detection of the calcitonin receptor-like receptor protein in the microvasculature of rat endothelium.
Calcitonin-gene-related peptide and adrenomedullin have similar and potent vascular effects, which appear to be mediated by the G protein-coupled calcitonin receptor-like (CRL) receptor. Using immunohistochemical and Western blot analyses, we have obtained novel evidence that CRL receptor is expressed in the rat vascular endothelium using an antibody to rat CRL receptor that we have raised and fully characterised. These results are an important basis for further studies aimed at determining the so far ill-defined functional significance of the extensive distribution of CRL receptor in the vascular endothelium.
Hagner
Stefanie
S
Institute of Physiology, University of Marburg, Deutschhaus Strasse 2, D-35037 Marburg, Germany. stefanie-hagner@online.de
Haberberger
Rainer
R
Hay
Deborah L
DL
Facer
Paul
P
Reiners
Katja
K
Voigt
Karlheinz
K
McGregor
Gerard P
GP
D000818:Animals; D058286:Calcitonin Receptor-Like Protein; D002460:Cell Line; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000098:blood supply* / Q000737:chemistry / Q000378:metabolism*; D008833:Microcirculation / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018003:Receptors, Calcitonin / Q000032:analysis* / Q000096:biosynthesis; D014162:Transfection
C545578:CALCRL protein, human; D058286:Calcitonin Receptor-Like Protein; C545580:Calcrl protein, rat; D018003:Receptors, Calcitonin