title: PTEN gene mutation and high MIB-1 labeling index may contribute to dissemination in patients with glioblastoma.
abstract: Dissemination of glioblastomas is often observed, but the underlying mechanism is not well clarified especially from the genetic viewpoints. The present study examined whether PTEN gene mutations and MIB-1 labeling index (LI) correlate with the dissemination in 39 consecutive patients with glioblastomas. Dissemination was defined as leptomenigeal enhancement by magnetic resonance imaging performed in all patients every 2-3 months. We examined PTEN mutations in 26 patients using cDNA-based direct sequencing and MIB-1 LI in 38 patients. Median survival time of the 39 patients was 16.2 months. Dissemination was found in 17 of 39 patients (43.6%). PTEN mutation was significantly associated with dissemination (P=0.0140), and higher MIB-1 LI (> or =35%) resulted in earlier dissemination (P=0.0156). Kaplan-Meier survival plots showed a significantly poorer survival for patients with PTEN mutation (P=0.0012). The results indicate that the evaluation of PTEN mutation and MIB-1 LI are useful to predict dissemination and prognosis of glioblastomas.
authors.affiliation: Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kato
authors.forename: Miki
authors.initials: M
authors.lastname: Fujimura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kumabe
authors.forename: Chikashi
authors.initials: C
authors.lastname: Ishioka
authors.forename: Ryunosuke
authors.initials: R
authors.lastname: Kanamaru
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshimoto
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D019656:Loss of Heterozygosity / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D019394:Ki-67 Antigen; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human