Validation and uses of a Y-chromosome STR 10-plex for forensic and paternity laboratories.
Y-chromosome short tandem repeats (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of male lineages. The present study validated a Y-STR 10-plex on the ABI PRISM 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid Cellmark. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. This study demonstrated that the 10-plex is sensitive to 0.125 ng of input DNA and that female samples mixed with male samples did not interfere with Y-STR haplotyping. In a sample of 525 males, there were three instances of locus multiplication, two at DYS19 and one at DYS435. Overall, haplotype diversity was 0.996, suggesting that the 10-plex can effectively distinguish among male lineages.
Johnson
Cassie L
CL
Orchid Cellmark-Dallas, Dallas, TX 75207, USA. cjohnson@orchid.com
Warren
Joseph H
JH
Giles
Robert C
RC
Staub
Rick W
RW
D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods*; D019075:Electrophoresis, Capillary; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences