Antiviral activity of lactoferrin against canine herpesvirus.
Lactoferrin (LF) is an iron-binding protein that is found in milk and other mammalian secretions. We found that bovine lactoferrin (bLF) inhibited both the in vitro infection and replication of canine herpesvirus (CHV) in Madin-Darby canine kidney (MDCK) cells. Incubation of CHV with bLF prevented subsequent infection of MDCK cells. Furthermore, proteins from CHV-infected MDCK cells were resolved by SDS-PAGE, and then bLF CHV-binding proteins were identified by far Western blotting. We demonstrated that the anti-CHV activity of bLF was due to its interaction with CHV as well as with MDCK cells. Both the apo- and holo-forms of bLF inhibited virus multiplication independently of their iron-withholding properties. We also demonstrated that human LF had anti-CHV activity. Our findings suggest that LF could be effective in dogs to provide protection against CHV infection.
Tanaka
Tetsuya
T
Dairy Science Laboratory, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan. tetsuya@anim.agr.hokudai.ac.jp
Nakatani
Syogo
S
Xuan
Xuenan
X
Kumura
Haruto
H
Igarashi
Ikuo
I
Shimazaki
Kei ichi
Ki
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D003207:Conalbumin / Q000494:pharmacology; D004285:Dogs; D002184:Herpesvirus 1, Canid / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D014168:Transferrin / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D014168:Transferrin; D014764:Viral Proteins; D003207:Conalbumin; D007781:Lactoferrin