Protein crystallization for genomics: throughput versus output.
Structural genomics involves many steps in order to reach from Gene to structure. This article focuses on the crystallization step in this chain of tasks. It is becoming increasingly evident that the current high throughput procedures for crystallising proteins do not always produce the expected output of high quality crystals required for structure determination by x-ray crystallography. This problem is discussed and suggestions for raising the output are presented.
Chayen
Naomi E
NE
Biological Structure and Function Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London SW7 2AZ, UK. n.chayen@imperial.ac.uk
D001671:Biochemistry / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D002627:Chemistry, Physical / Q000379:methods; D003460:Crystallization / Q000379:methods; D005782:Gels; D023281:Genomics / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000737:chemistry*
D005782:Gels; D011506:Proteins