Role of secretory and cytosolic phospholipase A(2) enzymes in lysophosphatidylcholine-stimulated monocyte arachidonic acid release.
To determine if lysophosphatidylcholine (lysoPC) is able to induce proinflammatory changes in monocytes, its ability to stimulate arachidonic acid (AA) release, a product of phospholipase A2 (PLA(2)) activity, has been analyzed. LysoPC increased AA release in THP-1 and Mono Mac6 cells in a time- and concentration-dependent manner. The monocytes expressed both secretory and cytosolic PLA(2) enzymes and AA release was strongly reduced by cellular pretreatment with different PLA(2) inhibitors and by pertussis toxin, an inhibitor of G(i)-protein activation. This indicates that both cytosolic and secretory PLA(2) enzymes regulate specific lysoPC receptor-induced AA release, suggesting lysoPC participation in monocyte proinflammatory activation.
Oestvang
Janne
J
Department of Biology, Section on Molecular Biology and Biotechnology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Anthonsen
Marit W
MW
Johansen
Berit
B
D016718:Arachidonic Acid / Q000737:chemistry / Q000378:metabolism*; D001095:Arachidonic Acids / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism; D063065:Organophosphonates / Q000494:pharmacology; D037342:Pertussis Toxin / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D054467:Phospholipases A2; D013997:Time Factors; D014316:Tritium
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D004791:Enzyme Inhibitors; D008244:Lysophosphatidylcholines; D063065:Organophosphonates; C104810:methyl arachidonylfluorophosphonate; D014316:Tritium; D016718:Arachidonic Acid; D019301:Oleic Acid; D037342:Pertussis Toxin; D010741:Phospholipases A; D054467:Phospholipases A2