Effect of Mycobacterium bovis BCG vaccination on Mycobacterium-specific cellular proliferation and tumor necrosis factor alpha production from distinct guinea pig leukocyte populations.
In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-alpha) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-alpha from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.
Lasco
Todd M
TM
Department of Medical Microbiology and Immunology, The Texas A&M University System-Health Science Center, College Station, Texas 77843, USA. Todd.Lasco@colostate.edu
Yamamoto
Toshiko
T
Yoshimura
Teizo
T
Allen
Shannon Sedberry
SS
Cassone
Lynne
L
McMurray
David N
DN
D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D006168:Guinea Pigs; D006358:Hot Temperature; D007962:Leukocytes / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000472:pathogenicity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014611:Vaccination
D001500:BCG Vaccine; D014409:Tumor Necrosis Factor-alpha