title: Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement.
abstract: The Arabidopsis thaliana (L.) Heyhn. genome contains 45 genes that show substantial sequence similarity to the tobacco ( Nicotiana tabacum L.) HIN1 (harpin-induced) gene and the Arabidopsis NDR1 (non-race-specific disease resistance) gene. Of these, the nine ( NHL1 to NHL3, NHL8 to NHL12 and NHL22) that bear the highest identity to HIN1 were selected and their expression in various situations was examined. We found that NHL10 behaves like the tobacco HIN1 gene in that its transcripts are abundant in senescing leaves and they specifically accumulate during the hypersensitive response (HR) caused by exposure to an avirulent Cucumber mosaic virus (CMV) strain. Spermine, a novel inducer of tobacco PR (pathogenesis-related) genes, was found to up-regulate expression of NHL3, NHL10 and NDR1. Green fluorescent protein-fusion experiments indicated that NHL2 and NHL10, and possibly NDR1 are localized in the chloroplasts. Studies using Arabidopsis mutants defective in salicylic acid (SA), ethylene and jasmonic acid signalling revealed that the senescence-associated expression of NHL10 is mediated by a pathway that involves SA but that NHL10 expression during CMV-induced HR and spermine treatment is totally independent of SA.
authors.affiliation: Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Miyagi, Japan.
authors.forename: Ming Shu
authors.initials: MS
authors.lastname: Zheng
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamamoto
authors.forename: Jyoti
authors.initials: J
authors.lastname: Shah
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Kusano
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D017799:Cucumovirus / Q000254:growth & development*; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D005030:Ethylenes / Q000378:metabolism / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D009154:Mutation; D009928:Organ Specificity / Q000235:genetics / Q000502:physiology; D054883:Oxylipins; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D011073:Polyamines / Q000494:pharmacology; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D000068103:Serine-Arginine Splicing Factors; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013096:Spermine / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; C109369:NDR1 protein, Arabidopsis; C486389:NHL10 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D011073:Polyamines; C473790:RCY1 protein, Arabidopsis; D014157:Transcription Factors; D000068103:Serine-Arginine Splicing Factors; D013096:Spermine; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid