title: Effects of serum deprivation on myofibrillar proteolysis in chick myotube cultures.
abstract: We examined the effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N(tau)-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B+L and D) activities were increased by serum deprivation. These results indicate that serum deprivation induces calpain, proteasome, and cathepsins activities, resulting in an increase in myofibrillar proteolysis in chick myotubes.
authors.affiliation: Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan. kaznaka@affrc.go.jp
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakashima
authors.forename: Itoko
authors.initials: I
authors.lastname: Nonaka
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Masaki
mesh_terms: D000818:Animals; D002154:Calpain / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002645:Chickens; D016895:Culture Media, Serum-Free; D003546:Cysteine Endopeptidases / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex
chemical_list: D016895:Culture Media, Serum-Free; D009097:Multienzyme Complexes; D009124:Muscle Proteins; D002403:Cathepsins; D002154:Calpain; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex