Identification and quantification of free radicals during myocardial ischemia and reperfusion using electron paramagnetic resonance spectroscopy.
There is general agreement that free radicals are involved in reperfusion injury. Electron paramagnetic resonance (EPR) spectroscopy can be considered as the more suitable technique to directly measure and characterize free radical generation during myocardial ischemia and reperfusion. There are essentially two approaches used in the detection of unstable reactive species: freezing technique and spin traps. The detection of secondary free radicals or ascorbyl free radicals during reperfusion might provide an index of oxidative stress. Spin trapping can also characterize nitric oxide. EPR spectroscopy can provide important data regarding redox state and free radical metabolism but ideally, the spin traps must not interfere with cell or organism function.
Vergely
Catherine
C
Laboratoire de Physiopathologie et Pharmacologie Cardiovasculaires Expérimentales, IFR no 100-Facultés de Médecine et de Pharmacie, 7 Bd Jeanne d'Arc-87900, 21079 Dijon Cedex, France.
Maupoil
Véronique
V
Clermont
Gaelle
G
Bril
Antoine
A
Rochette
Luc
L
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000097:blood* / Q000737:chemistry; D005615:Freezing; D006801:Humans; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D019097:Spin Trapping
D005609:Free Radicals; D009569:Nitric Oxide; D001205:Ascorbic Acid