A genotype-specific pollen gene associated with self-incompatibility in Lycopersicon peruvianum.
Self-incompatibility is a genetically controlled process used to prevent self-pollination. We report here the characterization of pollen cDNA clones of Lycopersicon peruvianum, and the identification of a genotype-specific pollen factor involved in self-incompatibility. To identify the latter, differential mRNA display RT-PCR was performed on pollen cDNAs from S12Sa and S11Sa genotypes. We isolated four cDNA fragments expressed preferentially in S12Sa pollen, and screened a cDNA library from S12Sa pollen with the four cDNA fragments to isolate the corresponding full length cDNAs. One of the four isolated cDNAs encoded part of an actin depolymerizing factor protein that we named LpADF. LpADF is highly homologous to actin depolymerizing factors of Arabidopsis thaliana, Lilium longiflorum, and Zea mays. RNA blot analysis revealed that LpADF is only expressed in mature pollen of the S12Sa genotype, and is therefore a candidate pollen factor in the gametophyte self-incompatibility system of L. peruvianum.
Kim
Myung Hee
MH
Faculty of Life Resources, Catholic University of Daegu, Gyeongsan 712-702, Korea.
Kim
Yang Suk
YS
Park
Soon Ki
SK
Shin
Dong Ill
DI
Park
Hee Sung
HS
Chung
Il Kyung
IK
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D015723:Gene Library; D017343:Genes, Plant; D005838:Genotype; D027762:Lilium / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D011058:Pollen / Q000235:genetics*; D016415:Sequence Alignment; D003313:Zea mays / Q000235:genetics
D010940:Plant Proteins