title: Ginsenoside Rb1: the anti-ulcer constituent from the head of Panax ginseng.
abstract: We previously reported that the butanol (BuOH) fraction of the head of Panax ginseng exhibited gastroprotective activity in peptic and chronic ulcer models. In order to identify the active constituent, an activity-guided isolation of the BuOH faction was conducted with a HCl x ethanol-induced gastric lesion model. The BuOH fraction was passed through a silica-gel column using a chloroform-methanol gradient solvent system, and six fractions (frs. 1-6) were obtained. The active fr. 5 was further separated by silica-gel column, to yield 6 subfractions (subfrs. a-f). Subfr. d was composed of ginsenosides Re, Rc and Rb1. The most active constituent was ginsenoside Rb1 (GRb1), a protopanaxadiol glycoside, which was investigated for its anti-ulcer effect. Gastric injury induced by HCl x ethanol, indomethacin and pyloric ligation (Shay ulcer) was apparently reduced with oral GRb1 doses of 150 and 300 mg/kg. GRb1 at these dosage significantly increased the amount of mucus secretion in an ethanol-induced model. The anti-ulcer effects were consistent with the result of histological examination. These results suggest that the major active constituent in the head of Panax ginseng is GRb1, and that anti-ulcer effect is produced through an increase in mucus secretion.
authors.affiliation: College of Pharmacy, Duksung Womens University, 419 Ssangmun-dong, Dobong-ku, Seoul 132-714, Korea. choonsik@duksung.ac.kr
authors.forename: Choon Sik
authors.initials: CS
authors.lastname: Jeong
authors.forename: Jin Ee
authors.initials: JE
authors.lastname: Hyun
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D005894:Panax; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000897:Anti-Ulcer Agents; D036145:Ginsenosides; D010936:Plant Extracts; C442759:ginsenoside Rb1