title: Recent advances in molecular epidemiology and detection of Taylorella equigenitalis associated with contagious equine metritis (CEM).
abstract: In the present review article, recent molecular advances relating to studies with Taylorella equigenitalis, as well as the recently described second species of the genus Taylorella, namely Taylorella asinigenitalis, have been described. Molecular genotyping of T. equigenitalis strains by pulsed-field gel electrophoresis (PFGE) after digestion with the suitable restriction enzyme(s) enabled the effective discrimination of strains, thus allowing the examination of the scientific mechanism(s) for its occurrence and transmission of contagious equine metritis (CEM). Alternatively, polymerase chain reaction (PCR) amplification and nucleotide sequencing of the 16S ribosomal DNA sequence and/or the other species specific sequence(s) as targets were confirmed to be effective for identification of T. equigenitalis. These new analytical methods at the genomic DNA level also enabled the discrimination of the newly discovered donkey-related T. asinigenitalis from T. equigenitalis, and moreover, the performance of phylogenetic analysis of genus Taylorella organisms with other closely related genera. Furthermore, detailed analysis of the genes responsible for CEM within the T. equigenitalis genome would be useful to help elucidate the pathogenic virulence and transmission mechanisms associated with the important equine pathogen associated with CEM.
authors.affiliation: Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagamihara 229-8501, Japan. matsuda@azabu-u.ac.jp
authors.forename: Motoo
authors.initials: M
authors.lastname: Matsuda
authors.forename: John E
authors.initials: JE
authors.lastname: Moore
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000453:epidemiology / Q000382:microbiology*; D006736:Horses; D008297:Male; D017720:Molecular Epidemiology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020625:Taylorella equigenitalis / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial