Immunocytochemical localization of neurons containing the AMPA GluR2/3 subunit in the hamster visual cortex.
AMPA glutamate receptors play a crucial role in brain functions such as synaptic plasticity and development. We have studied the chemo-architecture of the AMPA glutamate receptor subtype GluR2/3 in the hamster visual cortex by immunocytochemistry and compared it with the distribution of the calcium-binding proteins, calbindin D28K and calretinin. Anti-GluR2/3-immunoreactive (IR) neurons were predominantly located in layers II/III, V, and VI, and the majority of the labeled neurons were round or oval. However, many pyramidal cells in layer V were also labeled. Two-color immunofluorescence revealed that none of the GluR2/3-IR neurons contained calbindin D28 K or calretinin. Thus specific layers of neurons express the GluR2/3 subunit and these do not correlate with expression of calbindin D28K and calretinin.
Ryoo
Soo-Ryoon
SR
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
Ahn
Chang-Hyun
CH
Lee
Jae-Young
JY
Kang
Youn-Sil
YS
Jeon
Chang-Jin
CJ
D000818:Animals; D064032:Calbindin 2; D064026:Calbindins; D006224:Cricetinae; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry / Q000166:cytology*; D017966:Pyramidal Cells / Q000737:chemistry / Q000166:cytology*; D018091:Receptors, AMPA / Q000096:biosynthesis*; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis; D014793:Visual Cortex / Q000737:chemistry / Q000166:cytology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
D064032:Calbindin 2; D064026:Calbindins; D018091:Receptors, AMPA; D064030:S100 Calcium Binding Protein G; C104723:glutamate receptor ionotropic, AMPA 3; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C104722:glutamate receptor ionotropic, AMPA 2