Raman dye-labeled nanoparticle probes for proteins.
In this paper, we demonstrate how one can chemically design Raman dye-functionalized nanoparticle probes with specific protein-binding affinities and use these probes, coupled with surface-enhanced Raman scattering (SERS) spectroscopy, to perform multiplexed screening of protein-small molecule interactions and protein-protein interactions in a protein microarray format.
Cao
Y Charles
YC
Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Jin
Rongchao
R
Nam
Jwa-Min
JM
Thaxton
C Shad
CS
Mirkin
Chad A
CA
D019297:2,4-Dinitrophenol / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D004076:Digoxigenin / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry; D036103:Nanotechnology; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000911:Antibodies, Monoclonal; D006873:Hydroquinones; D011506:Proteins; D012834:Silver; D001710:Biotin; D004076:Digoxigenin; D019297:2,4-Dinitrophenol; C031927:hydroquinone