title: [Expression of telomerase and p53 in ameloblastoma].
abstract: OBJECTIVE
  To detect the expression of p53 protein, hTR, and hTERT mRNA in ameloblastoma (AB) and to investigate the clinical biological characteristics of AB.
  
  
  METHODS
  We detected hTR and hTERT Mrna in 54 cases of AB by in situ hybridization, and p53 protein by S-P method.
  
  
  RESULTS
  The positive ratio of hTR, hTERT mRNA was 2/7 and 1/7 in normal oral mucosa, 81.2%(13/16) and 87.4% (14/16) in odontogenic keratocyst (OKC), 94.4%(51/54) in AB, respectively. There was a significant difference in these three groups (P < 0.001). A strong correlation between hTR and hTERT mRNA was found (rs = 1.000, P < 0.001). p53 protein was expressed in 87.5% (42/49) AB, 44%(11/25) OKC, 33.3%(1/3) normal oral mucosa, respectively. A strong correlation between hTERT mRNA and p53 protein was found (rs = 0.848, P < 0.001). A moderate correlation between hTR mRNA and p53 protein was found (rs = 0.536, P < 0.001).
  
  
  CONCLUSIONS
  The positive ratios of hTR and hTERT were higher than p53 protein in AB. Telomerase activity and p53 expression play an important role in genesis and development of AB.
authors.affiliation: College of Stomatology, China Medical University, Shenyang 110002, China. zhongming1234@163.net
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Lu-huang
authors.initials: LH
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000737:chemistry* / Q000209:etiology; D002648:Child; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007573:Jaw Neoplasms / Q000737:chemistry* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000032:analysis* / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019098:Telomerase