Transmission of alien chromosomes from selfed progenies of a complete set of Allium monosomic additions: the development of a reliable method for the maintenance of a monosomic addition set.
Selfed progeny of a complete set of Allium fistulosum - Allium cepa monosomic addition lines (2n = 2x + 1 = 17, FF+1A-FF+8A) were produced to examine the transmission rates of respective alien chromosomes. All eight types of the selfed monosomic additions set germinable seeds. The numbers of chromosomes (2n) in the seedlings were 16, 17, or 18. The eight extra chromosomes varied in transmission rate (%) from 9 (FF+2A) to 49 (FF+8A). The complete set of monosomic additions was reproduced successfully by self-pollination. A reliable way to maintain a set of Allium monosomic additions was developed using a combination of two crossing methods, selfing and female transmission. FF+8A produced two seedlings with 18 chromosomes. Cytogenetical analyses, including GISH, showed that the seedlings were disomic addition plants carrying two entire homologous chromosomes from A. cepa in an integral diploid background of A. fistulosum. Flow cytometry analysis showed that a double dose of the alien 8A chromosome caused fluorescence intensity values spurring in DNA content, and isozyme analysis showed increased glutamate dehydrogenase activity at the gene locus Gdh-1.
Shigyo
M
M
Department of Biological and Environmental Science, Faculty of Agriculture, Yamaguchi University, Japan. shigyo@yamaguchi-u.ac.jp
Wako
T
T
Kojima
A
A
Yamauchi
N
N
Tashiro
Y
Y
D000490:Allium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001947:Breeding / Q000379:methods; D003433:Crosses, Genetic; D005298:Fertility / Q000235:genetics; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005969:Glutamate Dehydrogenase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D009006:Monosomy; D015203:Reproducibility of Results; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D007527:Isoenzymes; D005969:Glutamate Dehydrogenase