title: Mass spectrometric analysis of glucose-modified ribonuclease.
abstract: RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N epsilon-(carboxymethyl)lysine formation.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate Science Research Center, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, U.S.A.
authors.forename: W E
authors.initials: WE
authors.lastname: Cotham
authors.forename: D J S
authors.initials: DJ
authors.lastname: Hinton
authors.forename: T O
authors.initials: TO
authors.lastname: Metz
authors.forename: J W C
authors.initials: JW
authors.lastname: Brock
authors.forename: S R
authors.initials: SR
authors.lastname: Thorpe
authors.forename: J W
authors.initials: JW
authors.lastname: Baynes
authors.forename: J M
authors.initials: JM
authors.lastname: Ames
mesh_terms: D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D005947:Glucose / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000032:analysis* / Q000737:chemistry
chemical_list: D012260:Ribonucleases; D005947:Glucose