Involvement of nitric oxide in inflammation of ovaries in gilts.
NADPH-diaphorase (NADPH-d) and an inducible type of nitric oxide synthase (iNOS) were demonstrated in porcine ovaries after unilateral infusion of bacteria into the hilus of an ovary. In group I one ml of saline was infused into the hilus of each ovary from the 15th day to the 19th day of the estrous cycle. In group II one ml of bacterial suspension (10(9) colony forming units of Escherichia coli, Staphylococcus aureus and Corynebacterium pyogenes, in a proportion 1:1:1, respectively) in saline was infused into the hilus of one ovary on days corresponding to those of the control group (gr. I), whereas saline was infused into the contralateral ovary. The ovaries were collected on the 7th day of the next estrous cycle. In the bacteria-treated ovary, the activity of NADPH-d was higher in the endothelium of blood vessels, corpora lutea and follicular walls in comparison to that observed in the respective structures of the contralateral ovary. The highest activity of NADPH-d was found in the vascular endothelium in the bacteria-infused ovary. Vascular smooth muscle cells found in both ovaries of the bacteria-treated gilts were more intensely stained for NADPH-d than those in control animals. After bacteria administration, the intensity of NADPH-d reaction in all the structures of both ovaries in group II was higher than in control group. The strongest immunostaining for iNOS was observed in all structures of the bacteria-infused ovary. In the contralateral ovary, iNOS-immunoreactivity was weaker but still stronger than that in control group. The present results revealed that infusions of bacteria into the hilus of one ovary enhanced the activity of NADPH-d and immunoreactivity for iNOS in both porcine ovaries. However, the activity of both enzymes was higher in the bacteria-infused ovary than in the contralateral one. These data suggest that locally synthesized NO can mediate an inflammatory effect of bacteria in the porcine ovaries.
Jana
Barbara
B
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland. baja@pan.olsztyn.pl
Andronowska
Aneta
A
Kucharski
Jan
J
D000818:Animals; D001424:Bacterial Infections / Q000201:enzymology / Q000662:veterinary*; D003354:Corynebacterium Infections / Q000201:enzymology / Q000662:veterinary; D004927:Escherichia coli Infections / Q000201:enzymology / Q000662:veterinary; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation / Q000382:microbiology / Q000662:veterinary*; D009252:NADPH Dehydrogenase / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D010049:Ovarian Diseases / Q000382:microbiology / Q000662:veterinary*; D010053:Ovary / Q000201:enzymology; D013203:Staphylococcal Infections / Q000201:enzymology / Q000662:veterinary; D013553:Swine Diseases / Q000201:enzymology / Q000382:microbiology*
D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D009252:NADPH Dehydrogenase