Evidence for heme oxygenase-1 association with caveolin-1 and -2 in mouse mesangial cells.
The interaction of heme oxygenase-1 (HO-1) and caveolin in the cultured mouse mesangial cells (MMC) was investigated. In normal MMCs, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Upon treating the MMCs either with cadmium (Cd) or spermine NONOate (SPER/NO), expression of HO-1 mRNA and protein was increased. Caveolae rich membranous fractions from the MMCs treated with Cd or SPER/NO contained both HO-1 and caveolin-1 or caveolin-2. The experiments of immuno-precipitation showed complex formation between the HO-1 and caveolin-1 or caveolin-2 in the Cd treated MMCs. Confocal microscopic results also support co-localization of HO-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with HO-1 in caveolae suggested that caveolin could also play an important role in regulating the function of HO-1.
Jung
Nam-Hee
NH
Department of Pharmacology and Toxicology, College of Medicine, Inha University, Incheon, Korea.
Kim
Hong Pyo
HP
Kim
Bum-Rae
BR
Cha
Seok Ho
SH
Kim
Gyeong A
GA
Ha
Hunjoo
H
Na
Young Eun
YE
Cha
Young-Nam
YN
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000187:drug effects / Q000378:metabolism*; D011232:Chemical Precipitation; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000187:drug effects / Q000378:metabolism*; D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D051379:Mice; D018613:Microscopy, Confocal; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009589:Nitrogen Oxides; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology
C495541:Cav1 protein, mouse; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D008565:Membrane Proteins; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D002104:Cadmium; C091861:spermine nitric oxide complex; D013096:Spermine; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse