[Purification of human MMP-1 fusion protein and clinical detection of MMP-1 by its polyclonal antibody].
OBJECTIVE
To obtain the fusion protein of MMP-1 and the rabbit-anti-human matrix metalloproteinase-1(MMP-1) polyclonal antibody, and to detect the serum MMP-1 in patients by this polyclonal antibody.


METHODS
The fusion protein was purified by affinity chromatography. Two rabbits were immunized with the purified fusion protein, and the immune sera of rabbits were collected. Antibodies (IgG) obtained from the immune sera were purified by ammonium sulfate fractionation, and the indirect sandwich enzyme immunoassay was used to detect the serum MMP-1 in 24 samples of patients with chronic liver disease or cirrhosis and in 12 normal sera as controls.


RESULTS
The purified MMP-1 fusion protein was successfully obtained; the purified specific polyclonal antibodies of rabbit-anti-human MMP-1 were also obtained from the immune sera of the rabbits, and could respond to human MMP-1. Compared with the normal controls, the OD value of serum MMP-1 significantly increased in patients with chronic hepatitis or liver cirrhosis (P < 0.01).


CONCLUSIONS
The rabbit-anti-human MMP-1 polyclonal antibody may be used in the diagnosis of initial stage of hepatic fibrosis.
Hou
Jue
J
Institute of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Hu
Guo-ling
GL
Liu
Shuang-hu
SH
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D019694:Hepatitis B, Chronic / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D011993:Recombinant Fusion Proteins; D020781:Matrix Metalloproteinase 1