[In situ hybridization analysis of USF1 mRNA expression in odontoblasts].
OBJECTIVE
To detect and verify USF1 mRNA expression in odontoblasts.


METHODS
T-USF1 clone prepared previously was used as template and the desired USF1 cDNA segment was amplified by PCR with specific primers. The segment was labeled with digoxin as a probe and in situ hybridization was performed on the mounting of odontoblasts.


RESULTS
Evidently positive staining was shown in the cytoplasm of odontoblasts.


CONCLUSIONS
We verified the expression of USF1 mRNA in odontoblasts for the first time and provided evidence for further research.
Wu
Li-an
LA
Department of Pediatric Dentistry, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Wen
Ling-ying
LY
Yang
Fu-sheng
FS
Liu
Yong
Y
D004268:DNA-Binding Proteins; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D009804:Odontoblasts / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D014157:Transcription Factors / Q000235:genetics*; D051720:Upstream Stimulatory Factors
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors