title: An ascidian homolog of vertebrate iodothyronine deiodinases.
abstract: In all classes of vertebrates, the deiodination of the prohormone T(4) to T(3) represents an essential activation step in thyroid hormone action. The possible presence of iodothyronine deiodinase activity in protochordates has been demonstrated in vivo. Recent molecular cloning of the genomes and transcripts of several ascidian species allows further investigation into thyroid-related processes in ascidians. A cDNA clone from Halocynthia roretzi (hrDx) was found to have significant homology (30% amino acid identity) with the iodothyronine deiodinase gene sequences from vertebrates, including the presence of an in-frame UGA codon that might encode a selenocysteine (SeC) in the active site. Because it was not certain that the 3' untranslated region (UTR) contained a SeC insertion sequence (SECIS) element essential for SeC incorporation, a chimeric expression vector of the hrDx coding sequence and the rat deiodinase SECIS element was produced, as well as an expression vector containing the intact hrDx cDNA. COS, CHO, and HEK cells were transfected with these vectors, and deiodinase activity was measured in cell homogenates. Outer-ring deiodinase activity was detected using both T(4) and reverse T(3) as substrates, and activity was enhanced by the presence of the reductive cofactor dithiothreitol. The enzyme activity was optimal during incubation between 20 and 30 C (pH 6-7) and was strongly inhibited by gold-thioglucose. The Halocynthia deiodinase appears to be a high Michaelis-Menten constant (K(m)) enzyme (K(m) reverse T(3), 2 microM; and K(m) T(4), 4 microM). Deiodinase activity was completely lost upon the substitution of the SeC residue in the putative catalytic center by either cysteine or alanine. Transfection of the full-length hrDx cDNA produced deiodinase activity confirming the presence of a SECIS element in the 3'UTR, as revealed by the SECISearch program. In conclusion, our results show, for the first time, the existence of an ascidian iodothyronine outer-ring deiodinase. This raises the hypothesis that, in protochordates, the prohormone T(4) is activated by enzymatic outer-ring deiodination to T(3).
authors.affiliation: Department of Internal Medicine, Erasmus Medical Center, 3000 Rotterdam, The Netherlands.
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Shepherdley
authors.forename: Willem
authors.initials: W
authors.lastname: Klootwijk
authors.forename: Kazuhiro W
authors.initials: KW
authors.lastname: Makabe
authors.forename: Theo J
authors.initials: TJ
authors.lastname: Visser
authors.forename: George G J M
authors.initials: GG
authors.lastname: Kuiper
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics* / Q000378:metabolism*; D007668:Kidney / Q000166:cytology; D007700:Kinetics; D008969:Molecular Sequence Data; D007470:Monoiodotyrosine / Q000378:metabolism; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D011993:Recombinant Fusion Proteins; D012644:Selenium Radioisotopes; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity; D013974:Thyroxine / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology; D014561:Urochordata / Q000235:genetics*; D014714:Vertebrates
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D012644:Selenium Radioisotopes; D014284:Triiodothyronine; D007453:Iodide Peroxidase; D007470:Monoiodotyrosine; D013974:Thyroxine