title: Candida zemplinina sp. nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines.
abstract: Four yeast strains isolated from fermenting botrytized grape musts in the Tokaj wine region of Hungary are shown to represent a new osmotolerant and psychrotolerant species. The new species, Candida zemplinina (type strain 10-372(T)=CBS 9494(T)=NCAIM Y016667(T)), is closely related to Candida stellata, a yeast common on overripe grapes and in sweet fermenting wines. The sequence of the D1/D2 domain of the C. zemplinina 10-372(T) 26S rDNA shows 8.1 % sequence difference when compared to its counterpart in C. stellata CBS 157(T). In the conserved 5.8S gene of the ITS1-5.8S-ITS2 region the difference is 8 %. The D1/D2 domain differs only at two nucleotides from the homologous sequence of a yeast strain isolated from botrytized grapes in California, suggesting that C. zemplinina is a wine yeast that occurs in geographically distant localities.
authors.affiliation: Department of Genetics, University of Debrecen, Debrecen, Hungary.
authors.forename: Matthias
authors.initials: M
authors.lastname: Sipiczki
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000254:growth & development; D014920:Wine / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D000431:Ethanol; D005947:Glucose