Enzyme immunoassay of NSE and GFAP as the criterion of dynamic evaluation of the rat blood-brain barrier in perinatal hypoxic ischemic injury of the CNS.
Enzyme immunoassay of the serum neurospecific antigens (gliofibrillar acid protein and neurospecific enolase) was used for evaluation of the resistance of the blood-brain barrier in Wistar rats with perinatal hypoxia and ischemia of the CNS. Perinatal hypoxia and ischemia of the CNS was modeled by two methods: ligation of the common carotid artery in 7-day-old rats followed by 3.5-h hypoxic hypoxia or 15-min anoxic exposure of fetuses isolated via hysterectomy on day 21 of gestation. Enzyme immunoassay of serum gliofibrillar acid protein and neurospecific enolase in control an experimental rat pups was carried out once a week during 3 months. In controls serum levels of gliofibrillar acid protein and neurospecific enolase virtually did not change during postnatal development, while in animals with cerebral hypoxia and ischemia induced in fetuses by both methods serum concentration of neurospecific enolase sharply increased 1 week after the injury and increased on weeks 6 and 10. The content of gliofibrillar acid protein was maximum on week 1 and later considerably varied, the peaks of its concentrations observed on weeks 3 and 8 preceded the increase in neurospecific enolase activity in peripheral blood.
Chekhonin
V P
VP
V. P. Serbskii State Research Center of Social and Forensic Psychiatry.
Lebedev
S V
SV
Dmitrieva
T B
TB
Blinov
D V
DV
Gurina
O I
OI
Semenova
A V
AV
Volodin
N N
NN
D000818:Animals; D001812:Blood-Brain Barrier; D002138:Calibration; D002339:Carotid Arteries / Q000473:pathology; D002490:Central Nervous System / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000097:blood* / Q000378:metabolism; D000860:Hypoxia; D002534:Hypoxia, Brain / Q000473:pathology; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology*; D007124:Immunoenzyme Techniques; D007511:Ischemia; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein; D010751:Phosphopyruvate Hydratase