The expression of GFP under the control of fibroin promotor in primary ovarian cells of Antheraea pernyi.
The fibroin promoter can stably express foreign gene in lepidopteran cells. Total RNA was extracted from the gland of silkworm, Antheraea pernyi and the transcription initiation site of fibroin gene of A. pernyi was identified by RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE). The expression vector (pGFP-N2/Fib) was constructed by use of replacing the CMV promoter with the fibroin promoter. The results of visual screening under a fluorescent inverted microscope and Western blot analysis indicated that the GFP gene was expressed in the primary cells of ovary origins from A. pernyi.
Li
Wenli
W
Bioengineering Department, Dalian University of Technology, Dalian 116012, People's Republic of China.
Jin
Liji
L
Bu
Pengcheng
P
An
Lijia
L
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005349:Fibroins / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D004247:DNA; D005349:Fibroins