Effects of transfer from seawater to fresh water on the growth hormone/insulin-like growth factor-I axis and prolactin in the Tilapia, Oreochromis mossambicus.
The effect of freshwater (FW) transfer on growth and on the growth hormone (GH)/insulin-like growth factor-I (IGF-I) axis was examined in the tilapia, Oreochromis mossambicus. Tilapia were raised in seawater (SW) for 5 months and then transferred to FW for an additional 40 days. The growth rate of the fish transferred to FW was significantly reduced compared with the growth rate of fish that remained in SW. Plasma levels of GH were significantly elevated in FW-transferred fish, as were plasma IGF-I levels. Pituitary GH and liver IGF-I mRNA levels, on the other hand, were significantly reduced in the fish transferred to FW. There was a significant correlation between body mass and mRNA levels of GH and IGF-I, but not with plasma levels of GH and IGF-I. Fish transferred to FW had significantly higher prolactin (PRL)(177) levels than the SW control fish, although there was no difference in plasma PRL(188) levels. Consistent with the hyperosmoregulatory effects of PRL, mRNA levels of both PRL(177) and PRL(188) were significantly higher in FW-transferred fish than in the fish in SW. These results suggest that transferring tilapia from SW to FW activates the GH/IGF-I axis, but growth is still inhibited, possibly due to the greater metabolic cost of osmoregulation in FW than in SW.
Riley
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LG
Department of Zoology and Hawaii Institute of Marine Biology, University of Hawaii, PO Box 1346, Kaneohe, HI 96744, USA.
Hirano
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Grau
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EG
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D005618:Fresh Water; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D011388:Prolactin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012623:Seawater; D017210:Tilapia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D013006:Growth Hormone