title: HPLC analysis of some sulphur compounds in saliva: comparison between healthy subjects and periodontopathic patients.
abstract: BACKGROUND
  Saliva is an easily available biological material that is not commonly analysed in clinical chemistry, while it could give useful information especially in several oral diseases.
  
  
  METHODS
  In this work, the sulphur containing compounds cysteine, cysteinylglycine and glutathione were analysed in saliva of control subjects and periodontopathic subjects by a HPLC method. The detection limit of the method is 0.5, 0.1 and 0.1 micromol/l for cysteine, cysteinylglycine and glutathione, respectively, and it is linear up to 10 mmol/l.
  
  
  RESULTS
  the median values for the control group are 1.2 micromol/l for cysteine and glutathione and 0.4 micromol/l for cysteinylglycine while those of periodontopathic patients are significantly increased (4.4, 2.1 and 11.0 micromol/l for cysteine, cysteinylglycine and glutathione, respectively).
authors.affiliation: Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
authors.forename: Bruno
authors.initials: B
authors.lastname: Zappacosta
authors.forename: Armando
authors.initials: A
authors.lastname: Manni
authors.forename: Silvia
authors.initials: S
authors.lastname: Persichilli
authors.forename: Donata
authors.initials: D
authors.lastname: Scribano
authors.forename: Angelo
authors.initials: A
authors.lastname: Minucci
authors.forename: Daniele
authors.initials: D
authors.lastname: Lazzaro
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Sole
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis / Q000378:metabolism; D004151:Dipeptides / Q000032:analysis / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D013457:Sulfur Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D004151:Dipeptides; D013457:Sulfur Compounds; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine