title: Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa.
abstract: The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
authors.affiliation: CERMAV-CNRS (affiliated with Université Joseph Fourier), PO Box 53, F-38041 Grenoble Cedex 09, France.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Cioci
authors.forename: Edward P
authors.initials: EP
authors.lastname: Mitchell
authors.forename: Catherine
authors.initials: C
authors.lastname: Gautier
authors.forename: Michaela
authors.initials: M
authors.lastname: Wimmerová
authors.forename: Dvora
authors.initials: D
authors.lastname: Sudakevitz
authors.forename: Serge
authors.initials: S
authors.lastname: Pérez
authors.forename: Nechama
authors.initials: N
authors.lastname: Gilboa-Garber
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D037102:Lectins; C038692:adhesin, Pseudomonas; D002118:Calcium; D005690:Galactose