title: Using two photon microscopy to quantify enzymatic reaction rates on polymer beads.
abstract: Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports.
authors.affiliation: School of Chemistry, The University of Edinburgh, Edinburgh, UK EH9 3JJ.
authors.forename: Annie Y
authors.initials: AY
authors.lastname: Bosma
authors.forename: Rein V
authors.initials: RV
authors.lastname: Ulijn
authors.forename: Gail
authors.initials: G
authors.lastname: McConnell
authors.forename: John
authors.initials: J
authors.lastname: Girkin
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Halling
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D004058:Diffusion; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D017785:Photons; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism*; D000099166:Polyacrylamides
chemical_list: D000178:Acrylamides; D004800:Enzymes, Immobilized; D011092:Polyethylene Glycols; D011108:Polymers; D013820:Thermolysin; C113798:polyethyleneglycol-polyacrylamide copolymer; D000099166:Polyacrylamides