title: Electrochemical investigation on interaction between DNA with quercetin and Eu-Qu3 complex.
abstract: The interactions of quercetin (Qu) and Eu-Qu3 complex with calf thymus DNA were studied using cyclic voltammetry (CV) and double potential step chronocoulometry (DPSCC) at glass carbon electrode (GCE) for the surface method. The method is simple, convenient, reliable, reagent saving. Information such as intrinsic binding constant (K), and binding numbers (n) of bound species per DNA (bp), ratio (K(Ox)/K(Red)) of the binding constants for the oxidized and reduced forms of a bound species and interaction mode was obtained using dsDNA-modified GCE. Quercetin and Eu-Qu3 can both bind to DNA, but quercetin binds to DNA mainly by electrostatic attraction and the complex bind to DNA by both intercalation and electrostatic attraction. For the quercetin/dsDNA-modified GCE systems, a K of (3.80+/-0.3) x 10(4) M(-1), saturation coverage value (Gammas) of (2.28+/-0.2) x 10(-10) mol/cm2 and n of 1.2 were obtained. For the complex system, a saturation coverage value (Gammas) of 1.65 x 10(-10) mol/cm2 and n of 1.8 were obtained.
authors.affiliation: Department of Chemistry, Northwest Normal University, Lanzhou 730070, PR China. jwkang@nwnu.edu.cn
authors.forename: Jingwan
authors.initials: J
authors.lastname: Kang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhuo
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
authors.forename: Hongde
authors.initials: H
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D002244:Carbon; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005063:Europium / Q000737:chemistry* / Q000378:metabolism*; D005898:Glass; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011794:Quercetin / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009942:Organometallic Compounds; D005063:Europium; D002244:Carbon; D004247:DNA; C427203:calf thymus DNA; D011794:Quercetin