title: Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes.
abstract: S-Formylglutathione hydrolase (SFGH) has activity toward several xenobiotic carboxyesters and catalyses the final step of formaldehyde detoxification: the hydrolysis of S-formylglutathione to formate and glutathione. The Arabidopsis thaliana enzyme (AtSFGH) was crystallized in space group C2, with unit-cell parameters a = 128.5, b = 81.1, c = 94.3 A, beta = 93.3 degrees and three molecules in the asymmetric unit. A second crystal form of AtSFGH could be obtained by pressurizing the monoclinic crystals at 2 MPa for 30 min. The resulting space group is either P3(1)21 or P3(2)21, with unit-cell parameters a = 75.1, c = 92.8 A and one molecule in the asymmetric unit. Crystallographic data have been collected for both crystal forms to resolutions of 1.7 A for the monoclinic crystal and 1.6 A for the trigonal crystal. The structure has been solved by MAD phasing using a three-wavelength data set collected from a monoclinic crystal of selenomethionine-labelled AtSFGH.
authors.affiliation: CCLRC Daresbury Laboratory, Warrington, Cheshire WA4 4AD, England. k.mcauley@dl.ac.uk
authors.forename: Katherine E
authors.initials: KE
authors.lastname: McAuley
authors.forename: Ian
authors.initials: I
authors.lastname: Cummins
authors.forename: Miroslav
authors.initials: M
authors.lastname: Papiz
authors.forename: Robert
authors.initials: R
authors.lastname: Edwards
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Fordham-Skelton
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D011312:Pressure; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry*; D013869:Thiolester Hydrolases / Q000737:chemistry* / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D012645:Selenomethionine; D013869:Thiolester Hydrolases; C020149:s-formylglutathione hydrolase