[Research of immunization with recombinant plasmid pcDNA3-pacA and pcDNA3-pacP against dental caries in gnotobiotic rats. I: Measurement of specific anti-PAc S-IgA, IgG, valence].
OBJECTIVE
In this study, rats were immunized with pcDNA3-pacA and pcDNA3-pacP through submandibular gland, the valence of specific anti-PAc S-IgA,IgG were measured.


METHODS
Gnotobiotic rats were vaccinated with pcDNA3-pacA, pcDNA3-pacP and combination use of pcDNA3-pacA and pcDNA3-pacP through submandibular gland. The valence of specific anti-PAc S-IgA in salivary, IgG in serum were measured.


RESULTS
The specific anti-PAc S-IgA, IgG could be detected in all of recombinant plasmid groups. The level of salivary specific anti-PAc S-IgA and IgG in pcDNA3-pacA, pcDNA3-pacP, combination use of pcDNA3-pacA and pcDNA3-pacP groups were higher than negative control groups (P < 0.05).


CONCLUSIONS
The recombinant plasmid pcDNA3-pacA and pcDNA3-pacP could provoke specific mucosal immune responses as a novel immunogen against dental caries.
Cao
Fu-xian
FX
Oral Section, No.1 Affiliated Hospital of Dalian Medical University, Dalian 116011, China. caofuxian@163.com
Liu
Tian-jia
TJ
Yang
De-qin
DQ
Pan
Ju-li
JL
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D003731:Dental Caries / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005856:Germ-Free Life; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D008297:Male; D010957:Plasmids / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013295:Streptococcus mutans / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007071:Immunoglobulin A, Secretory; D019444:Vaccines, DNA