title: Thermodynamic treatment of oligonucleotide duplex-simplex equilibria.
abstract: Thermodynamic formulations have been devised to obtain Delta G degrees values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.
authors.affiliation: Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Owczarzy
authors.forename: Isard
authors.initials: I
authors.lastname: Dunietz
authors.forename: Mark A
authors.initials: MA
authors.lastname: Behlke
authors.forename: Irving M
authors.initials: IM
authors.lastname: Klotz
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Walder
mesh_terms: D001622:Betaine / Q000737:chemistry; D001669:Biochemical Phenomena; D001671:Biochemistry; D007477:Ions; D007700:Kinetics; D008962:Models, Theoretical; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012964:Sodium / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics
chemical_list: D007477:Ions; D009841:Oligonucleotides; D000644:Quaternary Ammonium Compounds; D001622:Betaine; D012964:Sodium