title: Colonial differentiation in Streptomyces coelicolor depends on translation of a specific codon within the adpA gene.
abstract: We identified adpA as an araC-like regulatory gene needed for colonial morphogenesis in Streptomyces coelicolor and showed that its activity depended on a unique TTA triplet corresponding to the leucyl-tRNA gene (bldA). These findings partially explained the dependence of aerial mycelium formation on a rare tRNA that is postulated to have developmental control functions.
authors.affiliation: Department of Molecular Microbiology, Biozentrum, Universität Basel, 4056 Basel, Switzerland.
authors.forename: Kien T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: Jennifer
authors.initials: J
authors.lastname: Tenor
authors.forename: Hansruedi
authors.initials: H
authors.lastname: Stettler
authors.forename: Lieu T
authors.initials: LT
authors.lastname: Nguyen
authors.forename: Liem D
authors.initials: LD
authors.lastname: Nguyen
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Thompson
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003062:Codon; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D025282:Mycelium / Q000235:genetics*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C401645:AdpA protein, Streptomyces griseus; D001426:Bacterial Proteins; C107344:BldA tRNA, Streptomyces coelicolor; D003062:Codon; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; C087736:RamR protein, Streptomyces; D015534:Trans-Activators; D014157:Transcription Factors