title: A novel ribonuclease from the veiled lady mushroom Dictyophora indusiata.
abstract: A ribonuclease (RNase), exhibiting a molecular mass of 28 kDa and specificity toward polyU and polyA and possessing an N-terminal sequence dissimilar to previously reported mushroom RNases, was isolated from dried fruiting bodies of veiled lady mushroom (Dictyophora indusiata). It demonstrated an RNase activity of 564 U/mg toward yeast transfer RNA. The RNase was adsorbed on DEAE-cellulose, CM-Sepharose, and Q-Sepharose. It demonstrated a pH optimum of 4-4.5 and a temperature optimum of 60 degrees C. There was a loss of RNase activity at temperatures above 60 degrees C.
authors.affiliation: Department of Microbiology, College of Biological Science, China Agricultural University, 10094 Beijing, China.
authors.forename: Hexing
authors.initials: H
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D011131:Polyribonucleotides / Q000737:chemistry*; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D017385:Sequence Homology
chemical_list: D005656:Fungal Proteins; D011131:Polyribonucleotides; D012260:Ribonucleases