title: The S-phase checkpoint and its regulation in Saccharomyces cerevisiae.
abstract: Cells are never more vulnerable than during DNA replication, which represents a major moment of potential genetic instability. Genotoxic insults induce many different forms of DNA damage that may interfere with the ability of cells to properly duplicate their genome. Primary damage may in turn undergo structural transformations during DNA replication, thus generating secondary lesions that may be even more dangerous. Cells experiencing replication of damaged DNA or replication blocks activate an S-phase checkpoint response that assures the fidelity and completion of DNA replication before cells enter M-phase. The S-phase checkpoint pathway regulates not only progress through the cell cycle but also DNA repair and DNA replication itself.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy. mariapia.longhese@unimib.it
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Longhese
authors.forename: Michela
authors.initials: M
authors.lastname: Clerici
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lucchini
mesh_terms: D004249:DNA Damage / Q000502:physiology; D004261:DNA Replication / Q000502:physiology*; D004271:DNA, Fungal / Q000502:physiology*; D016196:S Phase / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D004271:DNA, Fungal