title: [Grapevine viruses in Tunisia].
abstract: Tunisian grapevine culture is affected by many viruses caused by some phytovirus belonging to nepovirus, closterovirus and trichovirus groups. The present work deal with the economically important viroses identified in tunisian grapevines. We present here the development methods to detect these viruses in propagating material. The important viruses biologically, biochemically, serologically and using molecular techniques, characterised are: GFLV, GLRaV3 and GVB. The genetic polymorphism analysis was also carried and tunisian isolates were compared to previously described ones in literature.
authors.affiliation: Laboratoire de génétique et biologie moléculaire, Faculté des Sciences de Tunis, Campus universitaire, Le Belvedère, 1060 Tunis, Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Acheche
authors.forename: S
authors.initials: S
authors.lastname: Fattouch
authors.forename: S
authors.initials: S
authors.lastname: M'Hirsi
authors.forename: N
authors.initials: N
authors.lastname: Marzouki
authors.forename: M
authors.initials: M
authors.lastname: Marrakchi
mesh_terms: D017790:Closterovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014416:Tunisia; D014773:Virology / Q000379:methods*; D027843:Vitis / Q000821:virology*
chemical_list: D004279:DNA, Viral