Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins.
In order to improve our understanding of the colonization of the pulmonary tract of cystic fibrosis (CF) patients by Pseudomonas aeruginosa, 162 isolates from five different ecological origins were studied. The genetic features of each isolate were determined by random amplification of polymorphic DNA (RAPD) and by searching for eight virulence genes (six known virulence genes, algD, lasB, toxA, plcH, plcN and exoS, and two genes encoding putative neuraminidases, nan1 and nan2). Five RAPD groups were identified. Most of the CF isolates were distributed equally in three of these groups (RA, RB and RC). The CF isolates in RB were related to isolates from a wide variety of origins. The CF isolates in RA were related to a population composed of 65 % of the non-CF isolates from pulmonary tract infections. RC was mainly composed of CF isolates that were related to 30 % of isolates from plants. All genes except exoS and nan1 were present in all isolates. The exoS and nan1 virulence factor genes were most prevalent in CF isolates. exoS, which encodes exoenzyme S, was present in 94 % of CF isolates but also in 80 % of non-CF isolates from pulmonary tract infections. nan1, which encodes a putative neuraminidase, was found in 82.5 % of the isolates from group RC, which was composed largely of CF isolates. In conclusion, three major genogroups of P. aeruginosa isolates, each of which exhibits peculiar genetic features, are able to colonize CF patients. This may have different consequences on the outcome of pulmonary disease.
Lanotte
Philippe
P
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Watt
Stephane
S
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Mereghetti
Laurent
L
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Dartiguelongue
Nathalie
N
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Rastegar-Lari
Aziz
A
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Goudeau
Alain
A
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
Quentin
Roland
R
Département de Microbiologie Médicale et Moléculaire, EA 3250, Unité de Bactériologie, Faculté de Médecine, Tours, France.
D036002:ADP Ribose Transferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015139:Blotting, Southern; D002237:Carbohydrate Dehydrogenases / Q000235:genetics; D003550:Cystic Fibrosis / Q000150:complications* / Q000382:microbiology; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005098:Exotoxins / Q000235:genetics; D005260:Female; D000071677:Histidine Kinase; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics; D009439:Neuraminidase / Q000235:genetics; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics; D011552:Pseudomonas Infections / Q000150:complications / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D019105:Random Amplified Polymorphic DNA Technique; D012137:Respiratory System / Q000382:microbiology*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002237:Carbohydrate Dehydrogenases; D017931:DNA Primers; D004269:DNA, Bacterial; D005098:Exotoxins; D000071677:Histidine Kinase; D008666:Metalloendopeptidases; D009439:Neuraminidase; D011494:Protein Kinases; D017855:Transferases (Other Substituted Phosphate Groups); D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C020562:GDPmannose dehydrogenase; C477519:sphingomyelin synthase, Pseudomonas aeruginosa; C043859:pseudolysin, Pseudomonas aeruginosa