title: Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein.
abstract: The elucidation of the antigenic structure of the envelope proteins of Arteriviridae which includes lactate dehydrogenase-elevating virus (LDV) will provide further understanding of a mechanism of strict host cell specificity. To analyze the linkage between LDV envelope proteins, M/VP-2 and VP-3, which may play an important role in viral infectivity, we generated specific antibody against M/VP-2 that has not been reported in previous studies. A synthetic polypeptide corresponding to the C-terminal region of LDV strain C (LDV-C) ORF6, which encodes M/VP-2, was chemically synthesized and coupled to keyhole limpet hemocyanin (KLH). The peptide was immunogenic in rabbits and induced antibody specific for viral protein. Western blotting and immunofluorescence analysis of virion M/VP-2 in infected macrophages showed that the antibody was able to react specifically with authentic virion protein. The immunoreactive antibody against LDV M/VP-2 described in this study will be useful for further studies of the specific roles of the envelope proteins in arterivirus assembly and infectivity.
authors.affiliation: Department of Technical Support and Development, National Institute of Radiological Science, 4-9-1 Anagawa, Inage-ku, 263-8555, Chiba-shi, Japan. h_omoe@nirs.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Takahashi-Omoe
authors.forename: K
authors.initials: K
authors.lastname: Omoe
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kameoka
authors.forename: S
authors.initials: S
authors.lastname: Matsushita
authors.forename: T
authors.initials: T
authors.lastname: Inada
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D018174:Arterivirus Infections / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D007772:Lactate dehydrogenase-elevating virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D016366:Open Reading Frames; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; C096094:M-VP-2 protein, lactate dehydrogenase-elevating virus; D014759:Viral Envelope Proteins