[TLR4 mRNA expression and liver injury in LPS-induced mouse].
OBJECTIVE
To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).


METHODS
Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.


RESULTS
A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.


CONCLUSIONS
The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.
Liu
Xiao-wei
XW
Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
You
Yu
Y
Lu
Fang-gen
FG
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism*; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors