[Effects of aldosterone and spironolactone on the proliferation and collagen synthesis of hepatic stellate cells in rats].
OBJECTIVE
To investigate the effects of aldosterone and spironolactone on the proliferation and collagen synthesis of hepatic stellate cells.


METHODS
Rat HSC were incubated with aldosterone or spironolactone, an aldosterone receptor antagonist at different concentrations. The proliferation of HSC was measured by (3)H-thymidine incorporation, and the collagen synthesis by (3)H-Proline. The changes of HSC cycle were analyzed by FCM.


RESULTS
At dose of 10(-4) mol/L, ALD increased the incorporation of (3)H-TdR and (3)H-Pro (P < 0.05), but didn't at the lower doses. Spironolactone significantly inhibited HSC proliferation and collagen synthesis. The effective concentration was among 10(-4) mol/L - 10(-6) mol/L, and in dose-dependent manner. The percentage of G(1) phase of the cell cycle was significantly increased, and the cell proliferation suppression of spironolactone is associated with cell arrest in G(1) phase. Co-stimulation with aldosterone and spironolactone did not have significant effects on HSC proliferation and collagen synthesis compared with the control group.


CONCLUSIONS
The proliferation and collagen synthesis of HSC might be enhanced by ALD at higher concentration, but was inhibited by spironolactone.
Yao
Jin-feng
JF
Department of Gestrointerology, Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Yao
Xi-xian
XX
Fang
Hong-mei
HM
Cui
Dong-lai
DL
Bai
Wen-yuan
WY
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology*; D008103:Liver Cirrhosis / Q000473:pathology*; D051381:Rats; D013148:Spironolactone / Q000494:pharmacology*
D013148:Spironolactone; D000450:Aldosterone; D003094:Collagen