[Analysis of nuclear and mitochondrial genomes of transplastomic Salpiglossis sinuata plants obtained by transfer of transformed plastids from N. tabacum (+S. sinuata) cybrid].
New model system of plastid transformation has been proposed using a wild representative of Solanaceae family--S. sinuata. Earlier obtained cybrid plants N. tabacum (+ S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed S. sinuata plastome was transferred from N. tabacum (+ S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.
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Pariĭ
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Komarnitskiĭ
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Gleba
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IuIu
Kuchuk
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D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005818:Genetic Engineering; D030821:Plants, Genetically Modified / Q000235:genetics*; D018087:Plastids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D019657:Solanaceae / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D004272:DNA, Mitochondrial; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer