A paramagnetic CEST agent for imaging glucose by MRI.
The europium(III) complex of a DOTA-tetraamide ligand (DOTA = 1,4,7,10-tetraazacyclododecane-N,N',N' ',N' ''-tetraacetic acids) containing two phenyl boronate pendent arms binds glucose reversibly with an association constant of 383 M-1 at pH 7. Glucose binding results in slowing of water exchange between a single Eu(III)-bound water molecule and bulk water, and this can be imaged by MRI using chemical exchange saturation transfer (CEST) imaging sequence. This metabolite-responsive paramagnetic CEST agent responds to changes in glucose over the physiologically important range (0-20 mM), and thus it offers the possibility of high-sensitivity MR imaging glucose in tissues using bulk water protons as antenna.
Zhang
Shanrong
S
The Rogers Magnetic Resonance Center, Department of Radiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9085, USA.
Trokowski
Robert
R
Sherry
A Dean
AD
D001897:Boronic Acids / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D005063:Europium / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry
D001897:Boronic Acids; D003287:Contrast Media; D009942:Organometallic Compounds; D005063:Europium; D005947:Glucose