1-Cys peroxiredoxin knock-out mice express mRNA but not protein for a highly related intronless gene.
1-Cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine, is a unique antioxidant enzyme. We have generated mice in which the 1-cysPrx gene has been inactivated; they are viable and fertile. Mice have a highly related intronless gene (1-cysPrx-P1, GenBank accession number AF085220) with the same length of open reading frame (224 aa) as 1-cysPrx but located on a different chromosome. Since the product of this gene possibly could mimic 1-cysPrx function, we compared expression of 1-cysPrx and 1-cysPrx-P1 in mouse tissues by real-time polymerase chain reaction and Western blot. 1-cysPrx mRNA and protein were expressed in all mouse tissues that were examined with the highest expression level in lung. 1-cysPrx-P1 mRNA was expressed only in testis. In the 1-cysPrx 'knock-out' mouse, 1-cysPrx-P1 mRNA expression level was similar to the wild type but protein expression was not detected. Thus, mouse 1-cysPrx-P1 is an mRNA-expressed pseudogene that does not result in detectable protein in vivo.
Mo
Yiqun
Y
Institute for Environmental Medicine, University of Pennsylvania Medical Center, One John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104-6068, USA.
Feinstein
Sheldon I
SI
Manevich
Yefim
Y
Zhang
Qunwei
Q
Lu
Lu
L
Ho
Ye-Shih
YS
Fisher
Aron B
AB
D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009928:Organ Specificity; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics*; D054464:Peroxiredoxins; D016133:Polymerase Chain Reaction / Q000379:methods; D011544:Pseudogenes / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping
D017931:DNA Primers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D010544:Peroxidases; D054464:Peroxiredoxins