Prognostic significance of epithelial cells in the blood of patients with gynaecological malignancies.
BACKGROUND
Some studies have shown that the detection of epithelial cells in peripheral blood indicates minimal residual disease status. There is a lack of data concerning the prognostic relevance of the detection of epithelial cells in the blood of patients with solid tumors. Therefore, we conducted the present study to analyse the relationship between the detection of epithelial cells in the blood, "conventional" prognosis factors and overall survival in patients with gynaecological malignancies.


METHODS
In a prospective study we investigated blood samples of patients with benign and malignant gynaecologic diseases from 11/97 to 03/99. We used an immunocytochemical approach using cytokeratin-directed antibodies CK 8-18 in 135 and A45-B/B3 in another 123 patients for detection of epithelial cells. Follow-up was obtained by checking the local tumor register and contact with all patients included in the study.


RESULTS
There was no significant correlation between detection rate and tumour stage, grading or lymph node status. The median time of follow-up was 45.9 (range 0.7-63) months. There was no significant correlation between the detection rate of positive epithelial cells (method A + B) and overall survival.


CONCLUSIONS
The results of this study underline the need to further investigate the role of disseminated tumor cells in the blood and bone marrow of patients with gynaecological malignancies.
Sehouli
Jalid
J
Department of Obstetrics and Gynaecology, Charité, Campus Virchow-Klinikum, Medical Faculty of the Humboldt University, Augustenburger Platz 1, 13353 Berlin, Germany. sehouli@aol.com
Könsgen
Dominique
D
Nimpsch
Robert
R
Stengel
Dirk
D
Oskay
Gülten
G
Mustea
Alexander
A
Oertel
Joachim
J
Lichtenegger
Werner
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D005833:Genital Neoplasms, Female / Q000097:blood* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies; D015996:Survival Rate
D007633:Keratins