Ecological adaptation during incipient speciation revealed by precise gene replacement.
To understand the role of adaptation in speciation, one must characterize the ecologically relevant phenotypic effects of naturally occurring alleles at loci potentially causing reproductive isolation. The desaturase2 gene of Drosophila melanogaster is such a locus. Two geographically differentiated ds2 alleles underlie a pheromonal difference between the Zimbabwe and Cosmopolitan races. We used a site-directed gene replacement technique to introduce an allele of ds2 from the Zimbabwe population into Cosmopolitan flies. We show that the Cosmopolitan allele confers resistance to cold as well as susceptibility to starvation when the entire genetic background is otherwise identical. We conclude that ecological adaptation likely accompanies sexual isolation between the two behavioral races of D. melanogaster.
Greenberg
Anthony J
AJ
Department of Ecology and Evolution, The University of Chicago, Chicago, IL 60637, USA.
Moran
Jennifer R
JR
Coyne
Jerry A
JA
Wu
Chung-I
CI
D000222:Adaptation, Physiological; D000349:Africa; D000466:Alkadienes / Q000032:analysis; D000475:Alkenes / Q000032:analysis; D000483:Alleles; D000818:Animals; D005075:Biological Evolution; D001824:Body Constitution; D017691:Caribbean Region; D003080:Cold Temperature; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D017753:Ecosystem; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000502:physiology; D005260:Female; D018390:Gene Targeting; D017344:Genes, Insect; D005838:Genotype; D008297:Male; D010641:Phenotype; D010675:Pheromones / Q000502:physiology; D011110:Polymorphism, Genetic; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal; D013045:Species Specificity; D013217:Starvation; D014921:Wings, Animal / Q000033:anatomy & histology
C479924:5,9-heptacosadiene; C096032:7,11-heptacosadiene; D000466:Alkadienes; D000475:Alkenes; D029721:Drosophila Proteins; D010675:Pheromones; D044943:Fatty Acid Desaturases; C499595:desat2 protein, Drosophila