Application of a molecularly imprinted polymer for the effective recognition of different anti-epidermal growth factor receptor inhibitors.
A molecularly imprinted polymer (MIP) was prepared using (E)-piceatannol, a natural potential anti-epidermal growth factor receptor (EGFR) inhibitor, as the template and 4-vinylpyridine as the functional monomer. The template was isolated from a Chinese traditional Tibetan medicinal herb, Caragana jubata, by a solid-phase extraction procedure. The crude extract of this herb was loaded on the MIP column for the binding test, and two different compounds besides the template itself were specifically recognized by the polymer, which were identified to be butein and quercetin possessing potent anti-EGFR tyrosine kinase activities with IC(50) values of 10 and 15 microM, respectively. Affinity and selectivity for these inhibitors and another three compounds coexisting with the template in this herb were evaluated in the chromatographic mode. For the first time, the affinity of a molecularly imprinted polymer was investigated to be correlative to the bioactivities of the analytes. The chromatographic behavior of the analytes was consistent with their activity values: the more active inhibitor was retained longer on the MIP. This research work afforded us a new approach for the effective recognition of novel anti-EGFR inhibitors from herbs by using the MIP as the receptor mimic to assay the bioactivities of reserved components, which will be very helpful in the direct separation of lead candidates for anticancer drugs.
Zhu
LiLi
L
College of Chemistry and Molecular Engineering, Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100871, People's Republic of China.
Chen
Lirong
L
Xu
Xiaojie
X
D032602:Caragana; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D010936:Plant Extracts; D011108:Polymers; D066246:ErbB Receptors