Induction of CRMP-4 in striatum of adult rat after transient brain ischemia.
OBJECTIVE
To study the expression of collapsing response mediated protein-4 (CRMP-4) and nestin in the ischemic adult rat brain following transient brain ischemia.


METHODS
Brain ischemia was induced by transient left middle cerebral artery occlusion (MCAO) for 60 min in adult rats. The expression of CRMP-4, nestin and bromodeoxyuridine (BrdU) was analyzed by immunohistochemical method. The co-localization of CRMP-4 and nestin or BrdU was analyzed by double staining combined with confocal laser scanning microscopy.


RESULTS
CRMP-4, a marker of immature neuron, could be expressed in the ipsilateral striatum and cerebral cortex at 1st and 2nd week after the ischemia-reperfusion; nestin, a marker of neural stem cell, occurred in above regions from several hours to 2 weeks. CRMP-4 costained with nestin and with BrdU incorporation.


CONCLUSIONS
Neural stem cells may present in the striatum and cerebral cortex of adult rat and can be triggered to differentiate into newborn neuron there by ischemic brain trauma.
Liu
Peng-Chong
PC
National Laboratory of Medical Neurobiology, Fudan University Shanghai Medical College, Shanghai 200032, China. fysun@shmu.edu.cn
Yang
Zeng-Jin
ZJ
Qiu
Mei-Hong
MH
Zhang
Ling-Mei
LM
Sun
Feng-Yan
FY
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000150:complications / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley
C449760:Dpysl3 protein, rat; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D001973:Bromodeoxyuridine