Recent developments in micro-diffraction on protein crystals.
Protein crystallography micro-diffraction and micro small-angle X-ray scattering at a third-generation synchrotron radiation source undulator beamline both require a compromise on beam size and beam divergence. Micro small-angle X-ray scattering requires in addition an as-close-as-possible angular approach to the direct beam, which is mainly limited by the beam divergence at the sample position. Both techniques have been developed at the ESRF microfocus beamline in a complimentary way. The development of a dedicated microgoniometer in the frame of an EMBL/ESRF collaboration has revealed the potential of micro-diffraction for protein crystallography and is a step in the quest for user friendly instrumentation.
Riekel
Christian
C
European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble, France.
D004867:Equipment Design; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods*; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012399:Rotation; D017356:Synchrotrons / Q000295:instrumentation*; D014961:X-Ray Diffraction / Q000295:instrumentation* / Q000379:methods*
D011506:Proteins