Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry.
A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO(3), and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C(18) HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.
Ventura
Meritxell
M
Analytical Chemistry Department, Institut Químic de Sarrià, Ramon Llull University, Via Augusta 390, 08017 Barcelona, Spain. mvenben@iqs.es
Vallejos
Carlos
C
Anaya
Iván Atilio
IA
Broto-Puig
Francesc
F
Agut
Montserrat
M
Comellas
Lluís
L
D002851:Chromatography, High Pressure Liquid; D040503:Coffea / Q000737:chemistry*; D003069:Coffee / Q000737:chemistry*; D006358:Hot Temperature; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D011786:Quality Control; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D003069:Coffee; D009183:Mycotoxins; D009793:Ochratoxins; D010936:Plant Extracts; C025589:ochratoxin A