Prediction of tyrosine sulfation sites in animal viruses.
Post-translational modification of proteins by tyrosine sulfation enhances the affinity of extracellular ligand-receptor interactions important in the immune response and other biological processes in animals. For example, sulfated tyrosines in polyomavirus and varicella-zoster virus may help modulate host cell recognition and facilitate viral attachment and entry. Using a Position-Specific-Scoring-Matrix with an accuracy of 96.43%, we analyzed the possibility of tyrosine sulfation in all 1517 animal viruses available in the Swiss-Prot database. From a total of 97,729 tyrosines, we predicted 5091 sulfated tyrosine sites from 1024 viruses. Our site predictions in hemagglutinin of influenza A, VP4 of rotavirus, and US28 of cytomegalovirus strongly suggest an important link between tyrosine sulfation and viral disease mechanisms. In each of these three viral proteins, we observed highly conserved amino acid sequences surrounding predicted sulfated tyrosine sites. Tyrosine sulfation appears to be much more common in animal viruses than is currently recognized.
Lin
Henry C
HC
Section of Neurobiology, Physiology and Behavior, University of California, Davis, CA 95616, USA.
Tsai
Kevin
K
Chang
Brian L
BL
Liu
Justin
J
Young
Melinda
M
Hsu
Willy
W
Louie
Samuel
S
Nicholas
Hugh B
HB
Rosenquist
Grace L
GL
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry*; D002417:Cattle; D017124:Conserved Sequence; D004372:Ducks; D006389:Hemagglutinins, Viral / Q000737:chemistry*; D006736:Horses; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry*; D008969:Molecular Sequence Data; D019707:Receptors, Chemokine / Q000737:chemistry*; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods*; D017385:Sequence Homology; D013045:Species Specificity; D013431:Sulfates / Q000737:chemistry*; D034421:Sus scrofa; D014443:Tyrosine / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
D036022:Capsid Proteins; D006389:Hemagglutinins, Viral; D019707:Receptors, Chemokine; D013431:Sulfates; C411191:US28 receptor, Cytomegalovirus; C060339:VP4 protein, Rotavirus; D014764:Viral Proteins; D014443:Tyrosine