A simple and sensitive chemiluminescence method for the determination of tiopronin for a pharmaceutical formulation.
Here we report a rather simple and convenient chemiluminescence (CL) method for the determination of tiopronin. It was based that tiopronin could greatly enhance CL between H2O2 and luminol in a basic alkaline solution. Light emission is intense, and even with a simple setup a high sensitivity could be achieved. The linear range was 3 mM-500 nM with a detection limit of 200 nM. Singlet oxygen and hydroxyl radical were suggested to be produced in this reaction and was responsible for the CL of tiopronin. As a preliminary application, this simple method has been successfully applied into the determination of tiopronin in a pharmaceutical formulation.
Lu
Jianzhong
J
Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China. jzlu@shmu.edu.cn
Lau
Choiwan
C
Yagisawa
Shiroki
S
Ohta
Kazuko
K
Kai
Masaaki
M
D002626:Chemistry, Pharmaceutical; D008163:Luminescent Measurements; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D008625:Tiopronin / Q000032:analysis* / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D008625:Tiopronin