title: Effect of monotherapy and combination therapy with richlocaine on tissue hypoxia and activity of keratinocyte detoxifying systems in ischemic skin flap.
abstract: Treatment with richlocaine alone and, especially, in combination with antihypoxant energostim decreased the total content of hydroxyproline in the ischemic skin flap on day 3 after excision. Combination therapy with richlocaine and energostim normalized the redox potential in the energy supply system, improved antioxidant protection, and promoted the recovery of a balance between various components in the antioxidant system. These changes were not accompanied enhanced production of malonic dialdehyde. Our results suggest that combination therapy with richlocaine and energostim maintains the adaptive reserves of detoxifying systems in keratinocytes and prevents endotoxemia. Richlocaine primarily stimulates glycolytic synthesis of ATP, activates nonmitochondrial antioxidant enzymes, and increases RNase activity in lysosomes.
authors.affiliation: Krasnodar Research Center, Russian Academy of Medical Sciences, Krasnodar Krai Administration.
authors.forename: V L
authors.initials: VL
authors.lastname: Popkov
authors.forename: A V
authors.initials: AV
authors.lastname: Zadorozhnyi
authors.forename: V P
authors.initials: VP
authors.lastname: Galenko-Yaroshevskii
authors.forename: N A
authors.initials: NA
authors.lastname: Varazanashvili
authors.forename: V N
authors.initials: VN
authors.lastname: Meladze
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004579:Electron Transport; D019446:Endotoxemia / Q000378:metabolism; D006019:Glycolysis; D000860:Hypoxia; D007511:Ischemia; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D008928:Mitochondria / Q000473:pathology; D010084:Oxidation-Reduction; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D012867:Skin / Q000473:pathology; D013997:Time Factors
chemical_list: D000975:Antioxidants; D010880:Piperidines; C493511:richlocaine; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; C039786:malonic semialdehyde; D012260:Ribonucleases