[Construction of tissue engineering skin containing melanocytes].
OBJECTIVE
To construct a tissue engineering skin containing melanocytes by employing tissue engineering method.


METHODS
The keratinocytes, dermal fibroblasts and melanocytes were isolated and purified. Then the cells were used to construct a tissue engineering skin containing melanocytes. The location of melanocytes in the tissue engineering skin were detected by Dopa staining, transmission electron microscope (TEM) and S-100 immunohistochemical staining.


RESULTS
Melanocytes can be detected in the basal layer of the constructed tissue engineering skin. The results of TEM showed that the melanocytes were in good conditions.


CONCLUSIONS
The artificial skin containing melanocytes was successfully constructed in vitro and can be used to repair the full-thickness skin defects.
Liu
Yuan
Y
Department of Oral Histology & Pathology, Qin Du Stomatological College, Fourth Military Medical University, Xi'an, Shaanxi, P. R. China 710032.
Jin
Yan
Y
Wang
Xin-wen
XW
D000293:Adolescent; D001672:Biocompatible Materials; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D008544:Melanocytes / Q000166:cytology*; D012867:Skin / Q000166:cytology*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials