title: Immunohistolocalization of carbonic anhydrase isozyme (CA-VI) in bovine mammary glands.
abstract: The localization of bovine carbonic anhydrase isozyme VI (CA-VI) was examined immunohistochemically in bovine mammary glands during early lactation period (after 2-3 days of postpartum) and dry period (at about 2 months preparturition in adults), and young calves (at 30 and 150 days after birth) using specific CA-VI antiserum. The immunoreaction for anti-CA-VI antiserum was very weak in the mammary glands in young (prepubescent) calves. In dry period, CA-VI was also weakly expressed in secretory epithelial (acinar) and ductal cells. In contrast, the reaction was intense in mammary gland cells in early lactation period. Dot blotting analysis indicated that anti-CA-VI reacted positively to beastings and mature saliva, but weakly or not at all to milk during the dry period or calf saliva, respectively. The intense expression of CA-VI in the mammary glands in early lactation period might compensate for low levels of secretion from functionally and structurally immature salivary glands in young calves.
authors.affiliation: Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara-shi, Kanagawa, Japan.
authors.forename: Nobutsune
authors.initials: N
authors.lastname: Ichihara
authors.forename: Masao
authors.initials: M
authors.lastname: Asari
authors.forename: Tamae
authors.initials: T
authors.lastname: Kasuya
authors.forename: Etsuko
authors.initials: E
authors.lastname: Susaki
authors.forename: Kumi
authors.initials: K
authors.lastname: Matsui
authors.forename: Toshiho
authors.initials: T
authors.lastname: Nishita
authors.forename: Hajime
authors.initials: H
authors.lastname: Amasaki
mesh_terms: D000818:Animals; D030741:Carbonic Anhydrase IV / Q000302:isolation & purification*; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000302:isolation & purification; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D012469:Salivary Glands / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D030741:Carbonic Anhydrase IV