Concomitant distribution shift of glial GABA transporter and S100 calcium-binding proteins in the rat retina after kainate-induced excitotoxic injury.
The goal of this study was to elucidate the involvement of neuronal and glial calcium-binding proteins in the stimulation of gamma-aminobutyric acid (GABA) transport system by kainate-induced excitotoxicity in the rat retina. We used immunohistochemical method to assess the localization of GABA reuptake and calcium-binding proteins. After systemic administration of kainate, the neuronal GABA transporter does not show an association with calbindin D-28K. However, the localization of the GAT-3 transport system in Müller glial cells is closely correlated with the S100 proteins interacting with glial fibrillary acidic protein (GFAP) in response to kainate injury. Furthermore, we demonstrate that kainate-mediated excitotoxicity induced concomitant distribution shift of glial GABA transporter, S100 proteins and GFAP in the distal processes and endfeet of glial cells during the first 48 h.
Kim
Donghou
D
Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, South Korea.
Kim
Mi Jung
MJ
Lee
Jong Hwan
JH
Im
Jin Ok
JO
Won
Yu Jin
YJ
Yoon
Seung-Yong
SY
Hong
Hea Nam
HN
D000818:Animals; D064026:Calbindins; D002352:Carrier Proteins / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D050485:GABA Plasma Membrane Transport Proteins; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000633:toxicity*; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D027361:Organic Anion Transporters; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism*; D013997:Time Factors
D064026:Calbindins; D002352:Carrier Proteins; D018690:Excitatory Amino Acid Agonists; D050485:GABA Plasma Membrane Transport Proteins; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D064030:S100 Calcium Binding Protein G; D009418:S100 Proteins; D007608:Kainic Acid