title: High-throughput screening of pKa values of pharmaceuticals by pressure-assisted capillary electrophoresis and mass spectrometry.
abstract: A high-throughput pKa screening method based on pressure-assisted capillary electrophoresis (CE) and mass spectrometry (MS) is presented. Effects of buffer type and ionic strength on sensitivity and pKa values were investigated. Influence of dimethyl sulfoxide (DMSO) concentration present in the sample on effective mobility measurement was examined. A series of ten volatile buffers, covering a pH range from 2.5 to 10.5 with the same ionic strength, was employed. The application of volatile background electrolytes resulted in significant signal increase as compared with commonly used non-volatile phosphate buffers. In general, the CE/MS system provided a ten-fold higher sensitivity than conventional UV detection. The newly developed CE/MS method offers high-throughput capacity by pooling a number of compounds into a single sample. Simultaneous measurement of more than 50 compounds was readily achieved in less than 150 min. The measured pKa values are consistent with the published data obtained from the CE/UV method and are also in good agreement with data generated by other methods. Other advantages of using CE/MS for pKa screening are illustrated with typical examples, including poorly soluble compounds and non-UV-absorbing compounds.
authors.affiliation: DMPK & Bioanalytical Chemistry, AstraZeneca R&D, Mölndal, S-43183 Mölndal, Sweden. hong.wan@astrazeneca.com
authors.forename: Hong
authors.initials: H
authors.lastname: Wan
authors.forename: Anders G
authors.initials: AG
authors.lastname: Holmén
authors.forename: Yudong
authors.initials: Y
authors.lastname: Wang
authors.forename: Walter
authors.initials: W
authors.lastname: Lindberg
authors.forename: Marie
authors.initials: M
authors.lastname: Englund
authors.forename: Mats B
authors.initials: MB
authors.lastname: Någård
authors.forename: Richard A
authors.initials: RA
authors.lastname: Thompson
mesh_terms: D000465:Algorithms; D002021:Buffers; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004121:Dimethyl Sulfoxide; D004353:Drug Evaluation, Preclinical; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D017711:Nonlinear Dynamics; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012015:Reference Standards; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012997:Solvents; D004121:Dimethyl Sulfoxide