title: Chemotactic activity of CXCL5 in cerebrospinal fluid of children with bacterial meningitis.
abstract: CXCL5 (epithelial-cell-derived neutrophil-activating protein (ENA-)78) is a CXC-chemokine that specifically acts on neutrophils. To obtain insight into the extent of local presence and action of CXCL5 during bacterial meningitis, we measured its concentrations in cerebrospinal fluid (CSF) of patients with culture-proven bacterial meningitis (n=14), aseptic meningitis (n=6), and controls (n=32) and compared these results with levels of other CXC-chemokines, CXCL8- (interleukin-8) and CXCL1-related oncogene (growth-related oncogene (GRO)-alpha). Patients with bacterial meningitis had profoundly elevated CSF concentrations of all three chemokines. CXCL5 was not detectable in patients with aseptic meningitis or control subjects. CSF from patients with bacterial meningitis exerted chemotactic activity towards neutrophils, which was partially inhibited by neutralizing antibodies against CXCL5 and CXCL8, but not CXCL1. CSF from controls exerted minor chemotactic activity, which could be strongly enhanced by the addition of recombinant CXCL5, CXCL8 or CXCL1. During bacterial meningitis, CXCL5 is elevated in CSF, where it is involved in the recruitment of neutrophils to the central nervous system.
authors.affiliation: Laboratory of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands. p.j.zwijnenburg@amc.uva.nl
authors.forename: Petra J G
authors.initials: PJ
authors.lastname: Zwijnenburg
authors.forename: Henrica M A
authors.initials: HM
authors.lastname: de Bie
authors.forename: John J
authors.initials: JJ
authors.lastname: Roord
authors.forename: Tom
authors.initials: T
authors.lastname: van der Poll
authors.forename: A Marceline
authors.initials: AM
authors.lastname: van Furth
mesh_terms: D000293:Adolescent; D054360:Chemokine CXCL1; D054365:Chemokine CXCL5; D019743:Chemokines, CXC / Q000134:cerebrospinal fluid / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002648:Child; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000134:cerebrospinal fluid / Q000502:physiology; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000134:cerebrospinal fluid* / Q000502:physiology*; D007710:Klebsiella Infections / Q000134:cerebrospinal fluid / Q000276:immunology; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000276:immunology; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid* / Q000276:immunology; D008585:Meningitis, Meningococcal / Q000134:cerebrospinal fluid / Q000276:immunology; D008586:Meningitis, Pneumococcal / Q000134:cerebrospinal fluid / Q000276:immunology; D018375:Neutrophil Activation / Q000276:immunology
chemical_list: C475829:CXCL1 protein, human; C486263:CXCL5 protein, human; D054360:Chemokine CXCL1; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8