Crystallization of [Fe3S4]-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus.
Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 A, beta = 103.86 degrees, and diffract to 1.5 A resolution.
Nielsen
Michael S
MS
Department of Chemistry, Building 207, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Harris
Pernille
P
Christensen
Hans E M
HE
D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000706:statistics & numerical data; D004926:Escherichia coli / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009355:Neomycin / Q000378:metabolism; D020027:Pyrococcus furiosus / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D019843:Archaeal Proteins; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D009355:Neomycin