Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging.
Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.
Karakikes
I
I
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, U.K.
Barber
R E
RE
Morrison
I E G
IE
Fernández
N
N
Cherry
R J
RJ
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; C045084:invariant chain