Serial immunoprecipitation assays for interferon--(IFN)-beta antibodies in multiple sclerosis patients.
We devised a sensitive, radioimmunoprecipitation assay (RIPA) for anti-interferon (IFN)-beta-binding antibody (BAB) detection. Our RIPA showed good agreement with a reference RIPA (mean difference, -3.2 +/- 10.6 AU), and detected BAB to both IFN-beta-1a and IFN-beta-1b. Neutralizing antibodies to IFN-b (NAB) were also determined with a standard method. BAB and NAB were measured in 393 serum samples from 77 multiple sclerosis (MS) patients treated with IFN-beta-1a or -1b, who were studied over two years, and subsequently classified as responders and non-responders. BAB were found at higher concentrations, and more frequently detected, in IFN-beta-1b- than in IFN-beta-1a-treated patients, and, at highest titres, preferentially in patients who were positive for NAB. However, in our series of MS patients, both titres and frequency of detection of BAB or NAB did not differ between IFN-b responders and non-responders.
Lampasona
Vito
V
Department of Clinical Chemistry, San Raffaele Scientific Institute, Milan, Italy.
Rio
Jordi
J
Franciotta
Diego
D
Furlan
Roberta
R
Avolio
Carlo
C
Fazio
Raffaella
R
Lavolpe
Vito
V
Vincent
Angela
A
Comi
Giancarlo
G
Trojano
Maria
M
Montalban
Xavier
X
Martino
Gianvito
G
D001323:Autoantibodies / Q000097:blood*; D006801:Humans; D016899:Interferon-beta / Q000276:immunology*; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay / Q000379:methods*; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D016899:Interferon-beta