In vitro and short-term in vivo characteristics of a Kel-F thin film modified glucose sensor.
A new outer layer composition, consisting of polytetrafluoroethylene (PTFE), Kel-F oil, and Nafion, is suggested to minimize the detrimental effect of dissolved oxygen and to extend the linear response range of a glucose oxidase(GOx)-based sensor using nonconducting polymer. The morphology of Kel-F/PTFE/Kel-F/Nafion polymeric laminate was followed during fabrication by SEM. When Kel-F film was formed on the PTFE outer layer, the linear response was extended to 21 mM, at a sensitivity of 2.8 +/- 0.8 nA/mM mm2. We demonstrate that a sensor without Kel-F/PTFE/Kel-F/Nafion outer layer is relatively oxygen dependent, whereas by comparison a sensor with Kel-F/PTFE/Kel-F/Nafion outer layer is oxygen independent. The current of such a glucose sensor implanted in the subcutaneous tissue stabilized within 60 min, and the lag between blood glucose changes and sensor output was within 1 min. The in vivo characteristics of the glucose sensor described show great promise for one-point in vivo calibration.
Kang
Sun Kil
SK
Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul 110-744, Korea.
Jeong
Ran-A
RA
Park
Sejin
S
Chung
Taek Dong
TD
Park
Sunmin
S
Kim
Hee Chan
HC
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013997:Time Factors
D001786:Blood Glucose; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D011138:Polytetrafluoroethylene; D005949:Glucose Oxidase; D010100:Oxygen