title: Biochemical markers of sperm function: male fertility and sperm selection for ICSI.
abstract: The expression of a 70 kDa chaperone protein, HspA2 (formerly called CK-M), has been identified in mature human spermatozoa. The central role of HspA2 has been established, as the expression level of this protein is related to sperm cellular maturity, DNA integrity, chromatin maturity, chromosomal aneuploidy frequency and sperm function, including fertilizing potential. The spermiogenetic events of cytoplasmic extrusion and remodelling of the plasma membrane, which facilitate the formation of zona pellucida binding site(s) in human spermatozoa, are related. Finally, the presence of the hyaluronic acid (HA) receptor on the plasma membrane of mature sperm coupled with the HA-coated slide sperm-binding assay, facilitates the testing of infertile men and the selection of single mature spermatozoa for ICSI. Because mature spermatozoa have no residual cytoplasm, the HA-bound sperm fraction is also enriched in spermatozoa that are normal by the Kruger strict morphology method.
authors.affiliation: The Sperm Physiology and IVF Laboratories, Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA.
authors.forename: S
authors.initials: S
authors.lastname: Cayli
authors.forename: A
authors.initials: A
authors.lastname: Jakab
authors.forename: L
authors.initials: L
authors.lastname: Ovari
authors.forename: E
authors.initials: E
authors.lastname: Delpiano
authors.forename: C
authors.initials: C
authors.lastname: Celik-Ozenci
authors.forename: D
authors.initials: D
authors.lastname: Sakkas
authors.forename: D
authors.initials: D
authors.lastname: Ward
authors.forename: G
authors.initials: G
authors.lastname: Huszar
mesh_terms: D000782:Aneuploidy; D001665:Binding Sites; D001706:Biopsy; D002462:Cell Membrane / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002869:Chromosome Aberrations; D004247:DNA / Q000737:chemistry; D005260:Female; D005306:Fertilization; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D008953:Models, Anatomic; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure*; D013737:Testis / Q000473:pathology; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D002843:Chromatin; D018840:HSP70 Heat-Shock Proteins; C091879:HSPA2 protein, human; D006820:Hyaluronic Acid; D004247:DNA