title: [cDNAs cloning of SARS-CoV PUMC2 viral genome].
abstract: OBJECTIVE
  To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.
  
  
  METHODS
  Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.
  
  
  RESULTS
  The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.
  
  
  CONCLUSIONS
  These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fan
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Tan
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Ke
authors.initials: K
authors.lastname: Zou
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: An-ping
authors.initials: AP
authors.lastname: Ni
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins