title: Transduction of porcine enteropathogenic Escherichia coli with a derivative of a shiga toxin 2-encoding bacteriophage in a porcine ligated ileal loop system.
abstract: In this study, we have investigated the ability of detoxified Shiga toxin (Stx)-converting bacteriophages Phi3538 (Deltastx(2)::cat) (H. Schmidt et al., Appl. Environ. Microbiol. 65:3855-3861, 1999) and H-19B::Tn10d-bla (D. W. Acheson et al., Infect. Immun. 66:4496-4498, 1998) to lysogenize enteropathogenic Escherichia coli (EPEC) strains in vivo. We were able to transduce the porcine EPEC strain 1390 (O45) with Phi3538 (Deltastx(2)::cat) in porcine ligated ileal loops but not the human EPEC prototype strain E2348/69 (O127). Neither strain 1390 nor strain E2348/69 was lysogenized under these in vivo conditions when E. coli K-12 containing H-19B::Tn10d-bla was used as the stx1 phage donor. The repeated success in the in vivo transduction of an Stx2-encoding phage to a porcine EPEC strain in pig loops was in contrast to failures in the in vitro trials with these and other EPEC strains. These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions.
authors.affiliation: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary. tothi@vmri.hu
authors.forename: István
authors.initials: I
authors.lastname: Tóth
authors.forename: Herbert
authors.initials: H
authors.lastname: Schmidt
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dow
authors.forename: Anna
authors.initials: A
authors.lastname: Malik
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
authors.forename: Béla
authors.initials: B
authors.lastname: Nagy
mesh_terms: D000818:Animals; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000821:virology*; D007082:Ileum / Q000382:microbiology; D022641:Shiga Toxin 2 / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D014161:Transduction, Genetic; D016662:Virus Integration
chemical_list: D022641:Shiga Toxin 2