Effect of Sm(3+) ion on growth of Bacillus thuringiensis by microcalorimetry.
By using an LKB-2277 Bioactivity Monitor, cycle-flow method, the thermogenic curves of aerobic growth for Bacillus thuringiensis cry II strain at 28 degrees C have been obtained. The metabolic thermogenic curves of B. thuringiensis cry II contained two distinct patterns: the first reflects the changes during the bacterial growth phase and the second corresponds to the sporulation phase. From these thermogenic curves in the absence and presence of Sm(3+) ions, the thermokinetic parameters such as the growth rate constants k, the interval time tau(I), the maximum power P (max 1) and heat-output Q(log) for log phase, the maximum power P (max 2) and heatoutput Q(stat) for stationary phase, the heat-output Q(spor) for sporulation phase and total heat effects QT are calculated. Sm(3+) ion has promoting action on the growth of B. thuringiensis cry II in its lower concentration range; on the other hand, this ion has inhibitory action on the sporulation of B. thuringiensis in its higher concentration range. We also found that the effects of Sm(3+) ion on B. thuringiensis during the sporulation phase were far greater than that during the bacterial phase. It is concluded that the application of B. thuringiensis for controlling insecticides is not affected by the presence of the rare-earth elements in the environmental ecosystem.
Ruming
Zhao
Z
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Yi
Liu
L
Wenhua
Li
L
Ping
Shen
S
Songsheng
Qu
Q
Ziniu
Yu
Y
D001413:Bacillus thuringiensis / Q000187:drug effects* / Q000254:growth & development; D002151:Calorimetry / Q000379:methods*; D012493:Samarium / Q000494:pharmacology*
D012493:Samarium