Conformational analysis of melittin in solution phase: vibrational circular dichroism study.
The vibrational absorption and vibrational circular dichroism (VCD) spectra of melittin in D(2)O solutions at different pH values, different salt concentrations, or different 2,2,2-trifluoroethanol (TFE) concentrations are recorded in the amide I' (1850-1600 cm(-1)) region. Two models are used to simulate this peptide in different conditions, and a coupled oscillator program is used to obtain the calculated absorption and VCD spectra. This study indicates that melittin adopts a mixed structure in D(2)O solution at low pH, low salt concentration, or low TFE concentration. With an increase in pH, salt concentration, or TFE concentration, the structure changes to alpha-helix and further increases lead to aggregation. These results demonstrate the versatility of VCD in probing the conformations of peptides under different environmental perturbations.
Wang
Feng
F
Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
Polavarapu
Prasad L
PL
D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008555:Melitten / Q000737:chemistry*; D011189:Potassium Chloride; D011487:Protein Conformation; D012996:Solutions; D014270:Trifluoroethanol
D012996:Solutions; D008555:Melitten; D011189:Potassium Chloride; D014270:Trifluoroethanol