title: Fine-structure molecular typing of Irish Helicobacter pylori isolates and their genetic relatedness to strains from four different continents.
abstract: Genotyping of 74 Irish Helicobacter pylori isolates was performed at four different loci (vacA signal sequence and mid-region, insertion-deletion polymorphisms at the 3' end of the cag pathogenicity island, and cagA). The predominant vacA alleles and insertion-deletion motifs suggest an ancestral relationship between Irish isolates and either specific East Asian or Northern European strains. In addition, fluorescent amplified fragment length polymorphism-PCR genotyping and phylogenetic analysis of 32 representative Irish H. pylori isolates and 22 isolates from four different continents demonstrated that the Irish H. pylori isolates examined were weakly clonal and showed some association with both European and Asian isolates. These three genotyping techniques show that Irish H. pylori isolates have distinctive features that may have evolved in this insular European population.
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland. carrolli@tcd.ie
authors.forename: Ian M
authors.initials: IM
authors.lastname: Carroll
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmed
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Beesley
authors.forename: Aleem A
authors.initials: AA
authors.lastname: Khan
authors.forename: Sheikh
authors.initials: S
authors.lastname: Ghousunnissa
authors.forename: Colm A O
authors.initials: CA
authors.lastname: Moráin
authors.forename: Cyril J
authors.initials: CJ
authors.lastname: Smyth
mesh_terms: D000349:Africa; D017931:DNA Primers; D004251:DNA Transposable Elements; D005060:Europe; D017353:Gene Deletion; D005838:Genotype; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007194:India; D007494:Ireland; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D012703:Serotyping / Q000379:methods; D013020:South America
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements