[Suitability of SSCP-PCR analysis for molecular detection of quinolone resistance in Campylobacter jejuni].
Foodborne infections with Campylobacter spp. are increasing, especially antibiotic resistant strains are emerging. Quinolone resistant isolates can cause failure of therapy in severe clinical infections. Molecular characterisation is needed for the detection of resistant variants of C. jejuni. Therefore 23 isolates from poultry and human medicine as well as three control strains were tested for their minimal inhibitory concentration, their Single-Strand-Conformation-Polymorphism (SSCP)-PCR pattern (a method for the detection of resistance determining point mutations), and their sequence of the quinolone resistance determining region (QRDR). Six different SSCP types could be identified: two types for quinolone resistant isolates and other types containing so called silent mutations without influence on the resistance. A genotypic monitoring of the quinolone resistance in C. jejuni can be useful for the early detection of new resistance variants. As a screening method for detection of point mutations in the QRDR the SSCP-PCR can be applied. Compared to other genotypic methods the SSCP-PCR is less time and cost consuming and needs only standard technical equipment.
Beckmann
Lutz
L
Bundesinstitut für Risikobewertung, Berlin.
Müller
Monika
M
Luber
Petra
P
Schrader
Christina
C
Bartelt
Edda
E
Klein
Günter
G
D000818:Animals; D002169:Campylobacter Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics; D002645:Chickens / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011201:Poultry Diseases / Q000382:microbiology; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA
D015363:Quinolones