title: Immunohistochemical analysis of platelet-derived growth factor and its receptors in soft tissue malignant fibrous histiocytoma.
abstract: BACKGROUND
  Little information is available regarding the expression of platelet-derived growth factor (PDGF) isoforms and their receptors in soft tissue malignant fibrous histiocytoma (MFH).
  
  
  METHODS
  We investigated expression of PDGF isoforms and their receptors (PDGF-R alpha and -R beta) in 43 MFH tissue specimens using immunohistochemical techniques. Furthermore, we examined the correlation of PDGF expression in MFHs with proliferative activity assessed by MIB-1 immunohistochemical staining.
  
  
  RESULTS
  Positive cytoplasmic immunoreactivity for PDGF-AA, -BB and -AB was identified in tumor cells of 28 (66%), 4 (10%) and 26 (61%), respectively, of the 43 MFHs analyzed. Positive cytoplasmic immunoreactivity for PDGF-R alpha and -R beta was identified in tumor cells of 41 (95%) and 32 (74%), respectively, of the MFHs. Thirty-four (79%) MFHs coexpressed one or more PDGF isoforms and their corresponding receptors. In PDGF-AA immunostaining, MIB-indices in the high immunoreactivity group (> 10% of tumor cells) were significantly higher than those in the low immunoreactivity group (< 10% of tumor cells) (p = 0.031).
  
  
  CONCLUSIONS
  Our data provide evidence to support the presence of an autocrine/paracrine mechanism of proliferation control involving the PDGF ligand/receptor system in human MFHs.
authors.affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. yamatetu@med.kobe-u.ac.jp
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akisue
authors.forename: Takashi
authors.initials: T
authors.lastname: Marui
authors.forename: Ikuo
authors.initials: I
authors.lastname: Fujita
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Teruya
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hitora
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakatani
authors.forename: Keiko
authors.initials: K
authors.lastname: Nagira
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000502:physiology; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D020033:Protein Isoforms; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000096:biosynthesis*
chemical_list: D019394:Ki-67 Antigen; D010982:Platelet-Derived Growth Factor; D020033:Protein Isoforms; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta