title: FT-IR study of heterologous protein expression in recombinant Escherichia coli strains.
abstract: Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.
authors.affiliation: INFM UdR Milano-Bicocca, Department of Biotechnologies and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Diletta
authors.initials: D
authors.lastname: Ami
authors.forename: Loredana
authors.initials: L
authors.lastname: Bonecchi
authors.forename: Simona
authors.initials: S
authors.lastname: Calì
authors.forename: Gaetano
authors.initials: G
authors.lastname: Orsini
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Tonon
authors.forename: Silvia Maria
authors.initials: SM
authors.lastname: Doglia
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000032:analysis* / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000096:biosynthesis; D011994:Recombinant Proteins; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins