title: Automated multi-dimensional purification of tagged proteins.
abstract: The capacity for high throughput purification (HTP) is essential in fields such as structural genomics where large numbers of protein samples are routinely characterized in, for example, studies of structural determination, functionality and drug development. Proteins required for such analysis must be pure and homogenous and available in relatively large amounts. AKTA 3D system is a powerful automated protein purification system, which minimizes preparation, run-time and repetitive manual tasks. It has the capacity to purify up to 6 different His6- or GST-tagged proteins per day and can produce 1-50 mg protein per run at >90% purity. The success of automated protein purification increases with careful experimental planning. Protocol, columns and buffers need to be chosen with the final application area for the purified protein in mind.
authors.affiliation: R&D Separations, Amersham Biosciences AB, Björkgatan 30, 751 84 Uppsala, Sweden. jill.sigrell@amersham.com
authors.forename: Jill A
authors.initials: JA
authors.lastname: Sigrell
authors.forename: Pär
authors.initials: P
authors.lastname: Eklund
authors.forename: Markus
authors.initials: M
authors.lastname: Galin
authors.forename: Lotta
authors.initials: L
authors.lastname: Hedkvist
authors.forename: Pia
authors.initials: P
authors.lastname: Liljedahl
authors.forename: Christine Markeland
authors.initials: CM
authors.lastname: Johansson
authors.forename: Thomas
authors.initials: T
authors.lastname: Pless
authors.forename: Karin
authors.initials: K
authors.lastname: Torstenson
mesh_terms: D001331:Automation; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics; D021621:Imaging, Three-Dimensional; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012984:Software
chemical_list: D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D006639:Histidine; D005982:Glutathione Transferase