title: [Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment].
abstract: OBJECTIVE
  To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.
  
  
  METHODS
  Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.
  
  
  RESULTS
  The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.
  
  
  CONCLUSIONS
  Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts
authors.affiliation: Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
authors.forename: Yu-tao
authors.initials: YT
authors.lastname: Zhan
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Hong-song
authors.initials: HS
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Cong
authors.forename: Ran
authors.initials: R
authors.lastname: Fei
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology*; D004777:Environment; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D022781:Hepatocytes / Q000473:pathology*; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley