title: Caspase inhibition prevents staurosporine-induced apoptosis in CHO-K1 cells.
abstract: Apoptosis is a distinct form of programmed cell death that plays an important role in many biological processes. Although the phenotypes of apoptotic cells are well documented, little is known of the central mechanism leading to programmed cell death. Over the past few years, a number of ICE/CED-3 family proteases (also termed caspases) have been discovered and implicated as the common effectors of apoptosis. In this report, we demonstrate that induction of apoptosis in CHO-K1 cells by staurosporine, a broad spectrum inhibitor of protein kinases, results in an increase in DEVD-dependent protease activity. These events were followed by nuclear DNA fragmentation and cell death. Inhibition of the DEVD-cleaving activity by a synthetic tetrapeptide inhibitor DEVD-CHO, blocked staurosporine-induced downstream apoptotic phenotypes, such as morphological characteristics and DNA fragmentation. These results suggest that staurosporine-induced apoptosis in CHO-K1 cells is mediated through the CPP32/caspase-3-like cysteine proteases.
authors.affiliation: CLONTECH Laboratories, Inc., 1020 East Meadow Circle Palo Alto, CA 94303, USA. egzhang@clontech.com
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Yan
authors.forename: V
authors.initials: V
authors.lastname: Gurtu
authors.forename: C
authors.initials: C
authors.lastname: Spencer
authors.forename: S R
authors.initials: SR
authors.lastname: Kain