Determination of cocaine, benzoylecgonine and cocaethylene in human hair by solid-phase microextraction and gas chromatography-mass spectrometry.
The present work describes a highly precise and sensitive method developed to detect cocaine (COC), benzoylecgonine (BE, its main metabolite) and cocaethylene (CE, transesterification product of the coingestion of COC with ethanol) in human head hair samples. The method was based on an alkylchloroformate derivatization of benzoylecgonine and the extraction of the analytes by solid-phase microextraction (SPME). Gas chromatography-mass spectrometry (GC-MS) was used to identify and quantify the analytes in selected ion monitoring mode (SIM). The limits of quantification and detection (LOQ and LOD) were: 0.1 ng/mg for COC and CE, and 0.5 ng/mg for BE. Good inter- and intra-assay precision was observed. The dynamic range of the assay was 0.1-50 ng/mg. The method is not time consuming and was shown to be easy to perform.
de Toledo
Fernanda Crossi Pereira
FC
College of Pharmaceutical Sciences and Toxicology, University of São Paulo, Av. Professor Lineu Prestes 580 B13B, 05508-900, SP, Brazil.
Yonamine
Mauricio
M
de Moraes Moreau
Regina Lucia
RL
Silva
Ovandir Alves
OA
D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C005618:benzoylecgonine; C066444:cocaethylene; D003042:Cocaine