title: [Effects of aldosterone and spironolactone on the proliferation and collagen synthesis of hepatic stellate cells in rats].
abstract: OBJECTIVE
  To investigate the effects of aldosterone and spironolactone on the proliferation and collagen synthesis of hepatic stellate cells.
  
  
  METHODS
  Rat HSC were incubated with aldosterone or spironolactone, an aldosterone receptor antagonist at different concentrations. The proliferation of HSC was measured by (3)H-thymidine incorporation, and the collagen synthesis by (3)H-Proline. The changes of HSC cycle were analyzed by FCM.
  
  
  RESULTS
  At dose of 10(-4) mol/L, ALD increased the incorporation of (3)H-TdR and (3)H-Pro (P < 0.05), but didn't at the lower doses. Spironolactone significantly inhibited HSC proliferation and collagen synthesis. The effective concentration was among 10(-4) mol/L - 10(-6) mol/L, and in dose-dependent manner. The percentage of G(1) phase of the cell cycle was significantly increased, and the cell proliferation suppression of spironolactone is associated with cell arrest in G(1) phase. Co-stimulation with aldosterone and spironolactone did not have significant effects on HSC proliferation and collagen synthesis compared with the control group.
  
  
  CONCLUSIONS
  The proliferation and collagen synthesis of HSC might be enhanced by ALD at higher concentration, but was inhibited by spironolactone.
authors.affiliation: Department of Gestrointerology, Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Yao
authors.forename: Xi-xian
authors.initials: XX
authors.lastname: Yao
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Fang
authors.forename: Dong-lai
authors.initials: DL
authors.lastname: Cui
authors.forename: Wen-yuan
authors.initials: WY
authors.lastname: Bai
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology*; D008103:Liver Cirrhosis / Q000473:pathology*; D051381:Rats; D013148:Spironolactone / Q000494:pharmacology*
chemical_list: D013148:Spironolactone; D000450:Aldosterone; D003094:Collagen