title: The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA.
abstract: A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
authors.forename: Björn
authors.initials: B
authors.lastname: Sohlberg
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Huang
authors.forename: Stanley N
authors.initials: SN
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D026723:Polyadenylation; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012343:RNA, Transfer; D011062:Polynucleotide Adenylyltransferase; D011117:Polyribonucleotide Nucleotidyltransferase