The chicken pituitary expresses an ovoinhibitor-like protein in subpopulations of some, but not all, hormone-producing cell types.
Ovoinhibitor is a serine protease-inhibiting protein that was originally purified from egg whites. It is secreted by the oviduct under the control of estrogen and progesterone and it specifically inhibits serine proteinases such as trypsin and chymotrypsin. During recent attempts to raise monoclonal antibodies (Mabs) against chicken bursa of Fabricius proteins, one Mab was produced that specifically recognized chicken ovoinhibitor. This was the first demonstration of ovoinhibitor in an avian immune organ. We presently report on the expression of an ovoinhibitor-like molecule by the pituitary of the chicken as revealed by immunocytochemistry and RT-PCR. Immunofluorescent dual staining experiments using the mouse anti-ovoinhibitor Mab in conjunction with polyclonal antibodies against various hypophysial hormones revealed partial co-localization of an ovoinhibitor-like molecule with growth hormone (GH), luteinizing hormone (LH), and pro-opiomelanocortin (POMC), in a subset of the respective hormone producing cells. By contrast, no co-localization with prolactin (PRL) could be reliably demonstrated. RT-PCR of hypophysial mRNA using ovoinhibitor gene-specific primers yielded an amplicon that was 20% shorter than predicted on the basis of the published ovoinhibitor sequence. Sequencing revealed that of the represented exons only the central portion was expressed in the pituitary and that both 5' and 3' ends of each exon had been truncated. While expression of ovalbumin-like serine protease inhibitors (serpins) has been previously reported in the rat pituitary, to our knowledge, this is the first report of a Kazal-type serine protease inhibitor in the vertebrate neuroendocrine system.
Oubre
Cherie M
CM
Department of Biology, Texas A&M University, 2472 TAMU, College Station, TX 77843-2472, USA.
D'Hondt
Els
E
Moore
Randle W
RW
Hargis
Billy M
BM
Berghman
Luc R
LR
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000378:metabolism*; D015387:Egg Proteins, Dietary / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D013006:Growth Hormone / Q000032:analysis; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000032:analysis; D008969:Molecular Sequence Data; D010058:Oviposition; D010902:Pituitary Gland / Q000737:chemistry* / Q000378:metabolism*; D010907:Pituitary Hormones / Q000096:biosynthesis*; D011333:Pro-Opiomelanocortin / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D015387:Egg Proteins, Dietary; D010907:Pituitary Hormones; C004224:ovoinhibitor; D011333:Pro-Opiomelanocortin; D007986:Luteinizing Hormone; D013006:Growth Hormone