Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.
OBJECTIVE
To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.


METHODS
The whole-cell patch clamp technique was used.


RESULTS
Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms.


CONCLUSIONS
Pae inhibited I(Na) in mouse hippocampus neurons.
Zhang
Guang-Qin
GQ
State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Hao
Xue-Mei
XM
Chen
Shi-Zhong
SZ
Zhou
Pei-Ai
PA
Cheng
He-Ping
HP
Wu
Cai-Hong
CH
D000818:Animals; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D039821:Monoterpenes; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D015222:Sodium Channels / Q000378:metabolism*; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; D026941:Sodium Channel Blockers; D015222:Sodium Channels; C015423:peoniflorin; D006027:Glycosides