Continuous D-tagatose production by immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor.
D-Tagatose was continuously produced using thermostable L-arabinose isomerase immobilized in alginate with D-galactose solution in a packed-bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme amount, and substrate concentration were found to be optimal at 0.8 mm, 520/7 mm, 0.375 h(-1), 5.65 units, and 300 g/L, respectively. Under these conditions, the bioreactor produced about 145 g/L tagatose with an average productivity of 54 g tagatose/L x h and an average conversion yield of 48% (w/w). Operational stability of the immobilized enzyme was demonstrated, with a tagatose production half-life of 24 days.
Ryu
Se-Ah
SA
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Kim
Chang Sup
CS
Kim
Hye-Jung
HJ
Baek
Dae Heoun
DH
Oh
Deok-Kun
DK
D019747:Aldose-Ketose Isomerases / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry; D019149:Bioreactors; D002845:Chromatography / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D045302:Geobacter / Q000201:enzymology*; D020723:Glucuronic Acid / Q000737:chemistry; D006601:Hexoses / Q000138:chemical synthesis*; D006603:Hexuronic Acids / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size
D000464:Alginates; D004800:Enzymes, Immobilized; D006601:Hexoses; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase; C030192:tagatose; D005690:Galactose