[Localization and role of dendritic cells in rat kidney with renal ischemia/ reperfusion injury].
OBJECTIVE
To investigate the localization and the role of dendritic cells (DCs) in rat kidney with renal ischemia/reperfusion injury, and to explore the effect of anti-P-selectin Lectin-epidermal growth factor (EGF) domain monoclonal antibody (PsL-EGFmAb) on DCs.


METHODS
(1)Rat model of renal ischemia/reperfusion was established and rats were divided into treated group with PsL-EGFmAb (n=20) and untreated group (n=20), which included four sub-groups respectively according to reperfusion time (1-hour,3-hour, 6-hour, 24-hour), sham group (n=5) severed as control. CD1a(+)CD80(+)DC was observed with microscopy images method and P-selectin was analysed with immunohistochemistry.


RESULTS
(1)In renal tissues of untreated group, CD1a(+)CD80(+)DC was found in renal tubules, interstitium and vessels, especially in renal tubules and interstitium, but DCs were hardly observed in sham group (P<0.001). The number of CD1a(+)CD80(+)DC in 24-hour group was greater than those in other groups (P<0.01). The number of DCs was positively associated with blood urea nitrogen and serum creatinine (both P<0.05). (2)P-selectin content was increased significantly after ischemia/reperfusion 1 hour. (3)The contents of DC and P-selectin were decreased after treated (P<0.05 and P<0.01) in rat renal tissues.


CONCLUSIONS
DCs play an important role in immune pathogenesis after renal ischemia/reperfusion injury, which is related to renal immigration of DCs mediated by P-selectin. PsL-EGFmAb may inhibit local DCs immigration and accumulation in kidney.
Sun
Gui-zhi
GZ
Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhou
Tong
T
Zhang
Yu-mei
YM
Zhang
Dong-qing
DQ
Chen
Yu-ysng
YY
Hu
Qing-Shen
QS
Chen
Nan
N
D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D003713:Dendritic Cells / Q000502:physiology*; D007511:Ischemia / Q000276:immunology*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000276:immunology*
D018949:Antigens, CD1; D018122:B7-1 Antigen; C419393:CD1a antigen