The effect of laminin and its peptide SIKVAV on a human salivary gland myoepithelioma cell line.
We have demonstrated that the basement membrane regulates the myoepithelioma. We are now studying the effect of laminin, a basement membrane protein, in the morphology of a cell line (M1) derived from human salivary gland plasmacytoid myoepithelioma. These cells were grown inside a three-dimensional preparation of laminin-1. Phenotype differences were assessed by light and transmission electron microscopy. In addition, we analysed the effect of a molecular domain of laminin-1, the peptide SIKVAV, on M1 cells. This peptide was chosen because it is effective in cell proliferation and differentiation. M1 cells grown inside laminin-1 were mostly plasmacytoid-like, while cells treated by SIKVAV showed light and electron microscopic features of typical plasmacytoid cells. This peptide also modulated smooth-muscle actin expression in M1 cells. We demonstrated that laminin-1 and its derived peptide SIKVAV morphoregulates myoepithelioma cells in culture.
Capuano
A C T
AC
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo SP 05508-900, Brazil.
Jaeger
R G
RG
D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000494:pharmacology*; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000378:metabolism; D009208:Myoepithelioma / Q000473:pathology*; D009842:Oligopeptides / Q000494:pharmacology*; D012468:Salivary Gland Neoplasms / Q000473:pathology*
D007797:Laminin; D009842:Oligopeptides; C121346:laminin 1; C077583:seryl-isoleucyl-lysyl-valyl-alanyl-valinamide