title: The chemical modification of the essential groups of beta-N-acetyl-D-glucosaminidase from Turbo cornutus Solander.
abstract: The chemical modification of beta-N-acetyl-D-glucosaminidase (EC3.2.1.30) from Turbo cornutus Solander has been first studied. The results demonstrate that the sulfhydryl group of cysteine residues and the hydroxyl group of serine residues are not essential to the enzyme's function. The modification of indole group of tryptophan of the enzyme by N-bromosuccinimide (NBS) can lead to the complete inactivation, accompanying the absorption decreasing at 278 nm and the fluorescence intensity quenching at 335 nm, indicating that tryptophan is essential residue to the enzyme. The modification of amino group of lysine residue by formaldehyde and trinitrobenzenesulfonic acid also inactivates the enzyme completely. The results show that lysine and tryptophan are probably situated in the active site of the enzyme. The modification of the imidazole residue and carboxyl group leads to inactivate incompletely, indicating they are not the composing groups of the enzyme active center, and they are essential for maintaining the enzyme's conformation which is necessary for the catalytic activity of the enzyme.
authors.affiliation: The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China.
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Lin
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000118:Acetylglucosaminidase / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D012694:Serine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
chemical_list: D013438:Sulfhydryl Compounds; D012694:Serine; D006639:Histidine; D014364:Tryptophan; D000118:Acetylglucosaminidase