title: Ganglioside-dependent cell attachment and endocytosis of murine polyomavirus-like particles.
abstract: For murine polyomavirus (Py), previous studies suggest the cellular target is a terminal alpha2,3-linked sialic acid. Here, we investigate the binding and uptake of mouse polyomavirus-like particles (PyVLP) derived from bacterially expressed VP1. We find that in fibroblast 3T6 cells, binding of PyVLP was substantially reduced by sialidase treatment, but only moderately affected by protease treatment, suggesting glycolipids such as the sialic acid-containing gangliosides mediate cell attachment. We further tested the entry requirement of PyVLP using the ganglioside-deficient GM95 murine cell line, and find PyVLP binding and entry were reduced in these cells. Finally, we find that addition of gangliosides G(M1), G(D1a), and G(T1b) to GM95 cells restored cellular PyVLP binding and uptake. Taken together, results indicate that gangliosides function in PyVLP cell attachment and endocytosis.
authors.affiliation: Institute of Biochemistry, Swiss Federal Institute of Technology-Zurich, CH-8093, Zurich, Switzerland.
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Smith
authors.forename: Hauke
authors.initials: H
authors.lastname: Lilie
authors.forename: Ari
authors.initials: A
authors.lastname: Helenius
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D001667:Binding, Competitive; D036022:Capsid Proteins / Q000378:metabolism*; D002460:Cell Line; D004705:Endocytosis / Q000502:physiology*; D010450:Endopeptidases / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005732:Gangliosides / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009439:Neuraminidase / Q000494:pharmacology; D011120:Polyomavirus / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D001427:Bacterial Toxins; D036022:Capsid Proteins; D005732:Gangliosides; D006017:Glycolipids; D009439:Neuraminidase; D010450:Endopeptidases