title: Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients.
abstract: OBJECTIVE
  To investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.
  
  
  METHODS
  All six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.
  
  
  RESULTS
  All 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.
  
  
  CONCLUSIONS
  The unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, China.
authors.forename: Shuanzeng
authors.initials: S
authors.lastname: Wei
authors.forename: Tonghua
authors.initials: T
authors.lastname: Liu
authors.forename: Hongrui
authors.initials: H
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D044466:Asian People; D045744:Cell Line, Tumor; D002681:China; D003062:Codon; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*
chemical_list: D003062:Codon