title: [Typing of Salmonella by DNA-microarrays].
abstract: Microarrays (DNA-Chips) are miniaturized carriers on which many nucleic acid molecule probes such as oligonucleotides or PCR products are immobilized in a high density, and compactness. Homologue DNA hybridises with the immobilized complementary nucleic acid probes. This study gives after a short general introduction in the principle of DNA-microarrays an overview about published data on the field of typing of Salmonella by microarrays. An onset of a DNA-microarray developed by the National Reference Laboratory for Salmonella (NRL-Salm) will be introduced. By this new technique, it is possible to answer epidemiological questions as well as to find genes involved in certain biochemical processes, such as pathogenicity or resistance of salmonellae.
authors.affiliation: Bundesinsitut für Risikobewertung, Nationales Referenzlabor für Salmonellen, Berlin.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Malorny
authors.forename: Beatriz
authors.initials: B
authors.lastname: Guerra
authors.forename: Patric
authors.initials: P
authors.lastname: Zeltz
authors.forename: Katharina
authors.initials: K
authors.lastname: Rissler
authors.forename: Reiner
authors.initials: R
authors.lastname: Helmuth
mesh_terms: D000818:Animals; D015342:DNA Probes; D004352:Drug Resistance, Microbial; D015698:Genomic Library; D006801:Humans; D017720:Molecular Epidemiology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012475:Salmonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012478:Salmonella Food Poisoning / Q000175:diagnosis* / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes