title: Spatial pattern and temporal evolution of retinal oxygenation response in oxygen-induced retinopathy.
abstract: OBJECTIVE
  To determine the spatial pattern and temporal evolution of the change in retinal partial oxygen pressure (DeltaPO(2)) associated with a murine oxygen-induced retinopathy (OIR) model of retinal neovascularization (NV).
  
  
  METHODS
  On P7, newborn C57BL/6 mice were exposed to 75% oxygen until postnatal day (P)12, followed by recovery in room air until P17 or P34. Control mice remained in room air until P17 or P34. At P17 and P34, functional magnetic resonance imaging (MRI) and a carbogen inhalation challenge was used to measure retinal DeltaPO(2). Retinal avascularity, distance from the optic nerve head to the vascular edge in the peripheral retina, and NV incidence and severity were measured in retinas stained with adenosine diphosphatase (ADPase).
  
  
  RESULTS
  In P17 and P34 controls and in P34 OIR animals, retinas were fully vascularized without evidence of NV. In P17 OIR mice, there was a large central retinal capillary-free zone (22% +/- 3% of the entire retinal area, mean +/- SD) and 4 clockhours (range 1-7) of retinal NV at the border of the peripheral vascular and central acapillary retina in 100% (36/36) of the mice. In P17 OIR mice, retinal DeltaPO(2) over the vascularized far peripheral retina was not significantly (P > 0.05) different from the P17 control but was supernormal (P < 0.05) over the central capillary-free retina. However, no differences (P > 0.05) in retinal DeltaPO(2) were found between the P34 control and OIR groups.
  
  
  CONCLUSIONS
  A reversible supernormal DeltaPO(2) was found only over the central acapillary retina during the appearance of retinal NV in a mouse OIR model. The present data show the applicability of carbogen-challenge functional MRI to the study of retinal DeltaPO(2) in vivo in eyes that are too small for the use of existing techniques.
authors.affiliation: Department of Anatomy and Cell Biology, Wayne State University, 540 E. Canfield, Detroit, MI 48201, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Roberts
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Berkowitz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001081:Apyrase / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D018496:Hyperoxia / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D012160:Retina / Q000378:metabolism; D015861:Retinal Neovascularization / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D012171:Retinal Vessels / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D001081:Apyrase; D010100:Oxygen