Enhanced peroxidase activity of hemoglobin in a DNA membrane and its application to an unmediated hydrogen peroxide biosensor.
Hemoglobin can exhibit not only a direct electron transfer reacting after being entrapped in a DNA membrane, but also a greatly enhanced peroxidase activity toward the reduction of hydrogen peroxide. Based on the direct electrochemical property and nice enzymatic activity of the protein in a DNA membrane, a reagentless hydrogen peroxide biosensor was prepared. The peak current related to hydrogen peroxide was linearly proportional to its concentration in the range of 1.9 x 10(-6)-6.8 x 10(-4) mol L(-1). The detection limit was 1 x 10(-6) mol/L.
Shang
Libin
L
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
Sun
Ziyi
Z
Wang
Xingwu
X
Li
Genxi
G
D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008566:Membranes / Q000378:metabolism; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results
D006454:Hemoglobins; D004247:DNA; D006861:Hydrogen Peroxide; D009195:Peroxidase