Mass spectrometric analysis of glucose-modified ribonuclease.
RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N epsilon-(carboxymethyl)lysine formation.
Cotham
W E
WE
Department of Chemistry and Biochemistry, Graduate Science Research Center, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, U.S.A.
Hinton
D J S
DJ
Metz
T O
TO
Brock
J W C
JW
Thorpe
S R
SR
Baynes
J W
JW
Ames
J M
JM
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D005947:Glucose / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000032:analysis* / Q000737:chemistry
D012260:Ribonucleases; D005947:Glucose