Viable cell detection by the combined use of fluorescent glucose and fluorescent glycine.
The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.
Matsuoka
Hideaki
H
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan. bio-func@cc.tuat.ac.jp
Oishi
Kanenari
K
Watanabe
Masaaki
M
Kozone
Ikuko
I
Saito
Mikako
M
Igimi
Shizunobu
S
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D016980:Aeromonas hydrophila / Q000254:growth & development; D001419:Bacteria / Q000254:growth & development*; D003847:Deoxyglucose / Q000031:analogs & derivatives*; D004926:Escherichia coli / Q000254:growth & development; D005456:Fluorescent Dyes; D005998:Glycine / Q000031:analogs & derivatives*; D008089:Listeria monocytogenes / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D012486:Salmonella typhimurium / Q000254:growth & development
D005456:Fluorescent Dyes; D003847:Deoxyglucose; D009327:4-Chloro-7-nitrobenzofurazan; C098340:2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose; D005998:Glycine