title: Efficacy of a phytase derived from Escherichia coli and expressed in yeast on phosphorus utilization and bone mineralization in turkey poults.
abstract: A slope-response bioassay was conducted with male turkey poults to determine the sparing effect of P, based on improvements in bone mineralization in turkey poults, from 10 to 21 d of age when diets were supplemented with a novel phytase. Reference diets for calculation of the sparing effect of P contained 0.47, 0.55, 0.70, and 0.79% nonphytate phosphorus (NPP). Diets with varying dosages of a swine, Escherichia coli-derived AppA2 phytase (ECP) expressed in Pichia pastoris yeast (0, 250, 500, 750, and 1,000 U/kg) were added to the 0.47% NPP diet and improvements in bone mineralization determined the sparing effect of P supplied from ECP. Two additional reference diets were included that contained 500 U/kg from one of two commercial phytases (PA and PB) derived from Aspergillus and Peniophora. At 500 U/kg diet the ECP spared an additional 0.22% NPP (if calculated from tibia ash %), 0.18% NPP (if calculated from toe ash %), 0.24% NPP (if calculated from mg tibia ash), or 0.21% NPP (if calculated from mg toe ash). Phosphorus retention results validate bioassay results, in that 500 U ECP/kg resulted in 68.2% P being retained (0.49% of diet P retained) as compared with only 58.9% P being retained from the unsupplemented control diet (0.421% of diet P retained; P < 0.05).
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA. applegt@purdue.edu
authors.forename: T J
authors.initials: TJ
authors.lastname: Applegate
authors.forename: D M
authors.initials: DM
authors.lastname: Webel
authors.forename: X G
authors.initials: XG
authors.lastname: Lei
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage* / Q000235:genetics; D000375:Aging; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002113:Calcification, Physiologic; D004926:Escherichia coli / Q000201:enzymology*; D008297:Male; D008903:Minerals / Q000032:analysis; D010758:Phosphorus / Q000378:metabolism*; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000008:administration & dosage; D013977:Tibia / Q000737:chemistry; D014422:Turkeys / Q000502:physiology*; D015430:Weight Gain
chemical_list: D008903:Minerals; D011994:Recombinant Proteins; D010758:Phosphorus; C028705:bone ash; D010832:6-Phytase