GDNF and bFGF are differentially involved in glial cell differentiation and neurite bundle formation in cultures from chick embryonic ciliary ganglia.
We have shown that the neurotrophic factors glial cell line-derived neurotrophic factor (GDNF) and basic fibroblast growth factor (bFGF) exert different effects on glial cells in cultures from chick embryo ciliary ganglia. bFGF acts as a mitogen on glial cells, and induces their aggregation to neuronal bodies; after 48 h of culture no glial cells could be observed along neurites. GDNF has no proliferative role; in contrast, it promotes the expression of the differentiative marker O4 and the association of glial cell bodies to neurites to form robust bundles.
Zamburlin
Pollyanna
P
Dipartimento di Biologia Animale e dell'Uomo, I-10123 Turin, Italy.
Gilardino
Alessandra
A
Ariano
Paolo
P
Lovisolo
Davide
D
Distasi
Carla
C
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology* / Q000502:physiology; D005724:Ganglia / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors / Q000494:pharmacology* / Q000502:physiology; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology
D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D016222:Fibroblast Growth Factor 2