Immunogenicity of Borrelia burgdorferi arthritis-related protein.
Immunization against arthritis-related protein (Arp) elicits antibody in mice that resolves arthritis but is not protective against challenge with Borrelia burgdorferi. In mice immunized against Arp, an unrelated 37-kDa protein (P37-42), outer surface protein A (OspA), or glutathione S-transferase (GT) and then challenged by syringe or tick, only OspA conferred protection. Passive transfer of Arp antiserum into infected SCID mice induced arthritis resolution, but antisera to P37-42, OspA, GT, or six overlapping Arp peptide fragments did not. Results suggest that the arthritis-resolving immunogenicity is specific to Arp, but the relevant epitopes may be conformational.
Feng
Sunlian
S
Center for Comparative Medicine, School of Medicine, University of California at Davis, 95616, USA.
Hodzic
Emir
E
Freet
Kimberly
K
Barthold
Stephen W
SW
D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage* / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001168:Arthritis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines; D025065:Borrelia burgdorferi / Q000276:immunology*; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D007116:Immunization, Passive; D008074:Lipoproteins; D008193:Lyme Disease / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D008809:Mice, Inbred C3H
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C072045:OspA protein; D005982:Glutathione Transferase