title: The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa.
abstract: The objective of this study was to assess the efficacy of a novel intracytoplasmic sperm injection (ICSI) procedure, as well as the in vitro and in vivo developmental competence of goat embryos produced by ICSI. Oocyte-cumulus complexes recovered by LOPU from donors stimulated with gonadotrophins were matured in vitro. Fresh goat semen was used for ICSI following Percoll gradient washing. Tail-cut spermatozoa were microinjected into the ooplasm of goat oocytes using a piezo micropipette-driving system (PiezoDrill). In order to assess developmental competence, the ICSI-derived zygotes were cultured in one of two media systems (mTALP-mKSOM vs G1.3-G2.3) for in vitro development or were transferred into recipients for full-term development. The results suggest that cutting sperm tails using the oocyte-holding pipette coupled with the PiezoDrill is an efficient approach for goat ICSI in terms of oocyte survival, pronuclear development and initial cleavage. The mTALP-mKSOM culture system was more suitable for in vitro development of ICSI-derived goat embryos than G1.3-G2.3. This first report of full-term development of an ICSI-derived goat embryo suggests that ICSI can be applied to assisted reproduction in goats.
authors.affiliation: Nexia Biotechnologies Inc., 1000, Avenue St-Charles, Vaudreuil Dorion, Quebec, Canada J7V 8P5. bwang@nexiabiotech.com
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Baldassarre
authors.forename: J
authors.initials: J
authors.lastname: Pierson
authors.forename: F
authors.initials: F
authors.lastname: Cote
authors.forename: K M
authors.initials: KM
authors.lastname: Rao
authors.forename: C N
authors.initials: CN
authors.lastname: Karatzas
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D006041:Goats; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013082:Sperm Tail; D013094:Spermatozoa / Q000502:physiology