Reprogramming the genome: role of the cell cycle.
In nuclear transfer reconstructed embryos, the co-ordination of donor nuclear and recipient cytoplasmic cell cycle phases is essential to maintain ploidy and prevent DNA damage. However, the stage of the cell cycle at the time of reconstruction and the method of reconstruction may also have a significant impact on the subsequent development of the embryo and fetus through a number of other mechanisms. This paper reviews some of the information currently available and proposes that consideration of the cell cycle may lead to improvement of methods for embryo reconstruction.
Campbell
K H S
KH
University of Nottingham, School of Biosciences, Sutton Bonington, Loughbrough, Leicestershire LE12 5RD, UK. keith.campbell@nottingham.ac.uk
Alberio
R
R
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002843:Chromatin / Q000235:genetics; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D006801:Humans; D008322:Mammals / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy
D002843:Chromatin