In vitro antiviral activity of prodelphinidin B-2 3,3'-di-O-gallate from Myrica rubra.
In this study, the in vitro antiviral properties of prodelphinidin B-2 3,3'-di- O-gallate (PB233'OG) isolated from the bark of Myrica rubra (Myricaceae) was investigated. Results showed that PB233'OG exhibited anti-herpes simplex virus type 2 (HSV-2) activity with IC (50) values of 5.3 +/- 0.1 and 0.4 +/- 0.04 microM for XTT and plaque reduction assays, respectively. The IC (50) value increased with increasing MOI (multiplicity of infection). PB233'OG did not show a cellular cytotoxic effect at concentrations that possessed antiviral activity. Mechanistic studies demonstrated that PB233'OG inhibited HSV-2 attachment to the Vero cell, interfered with the penetration of HSV-2 into the Vero cell, affected the late stage(s) of the HSV-2 infection cycle, and also reduced the viral infectivity at high concentrations. It is concluded that PB233'OG exhibits various modes of action in its anti-HSV-2 effects.
Cheng
Hua-Yew
HY
Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
Lin
Ta-Chen
TC
Ishimaru
Kanji
K
Yang
Chien-Min
CM
Wang
Kuo-Chih
KC
Lin
Chun-Ching
CC
D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002522:Chlorocebus aethiops; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D031641:Myrica; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D014709:Vero Cells / Q000821:virology
D000872:Anthocyanins; D000998:Antiviral Agents; D010936:Plant Extracts; C472180:prodelphinidin B-2 3'-O-gallate