title: [Analysis on the SARS-CoV genome of PUMC01 isolate].
abstract: OBJECTIVE
  To perform variation and phylogenetics analysis on the SARS-CoV genome sequence (PUMC01) isolated in the Peking Union Medical College Hospital.
  
  
  METHODS
  The cDNA library of SARS-CoV (PUMC01 isolate) was constructed by means of random-priming strategy. Random selected plasmid was sequenced and the genome sequence of SARS-CoV-PUMC01 was assembled by conventional methods (The Genebank Accession No. of SARS-CoV-PUMC01 is AY350750). The variation and phylogenetics analysis were performed by comparing the PUMC01 sequence with other SARS-CoV isolates.
  
  
  RESULTS
  Ten variation sites were found by comparing PUMC01 isolate with Tor2 and Urbani isolates. In phylogenetic analysis of 18 SARS-CoV isolates, two classes were observed and there is different differential time between these two classes and the different isolates in each class.
  
  
  CONCLUSIONS
  The evidence of phylogenetic analysis of different SARS-CoV isolates from different region is instructive for understanding the clinical relations between the different isolates and the transmission chain of SARS-CoV.
authors.affiliation: Chinese Academy of Medical Sciences-Chinese National Human Genome Center, Beijing SARS Coronavirus Genome Sequence Research Group, Beijing 100005, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zou
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Ke-yue
authors.initials: KY
authors.lastname: Ding
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Guo-zhu
authors.initials: GZ
authors.lastname: Wei
authors.forename: Xin-feng
authors.initials: XF
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhan-xia
authors.initials: ZX
authors.lastname: Yu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fan
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Peng
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Xiang-jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: An-ping
authors.initials: AP
authors.lastname: Ni
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins