title: Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students.
abstract: Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Antoinette
authors.initials: A
authors.lastname: Ngandjio
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Maithe
authors.initials: M
authors.lastname: Clerc
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Marie Christine
authors.initials: MC
authors.lastname: Fonkoua
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Thonnon
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Lunel
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Christiane
authors.initials: C
authors.lastname: Bébéar
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bianchi
authors.affiliation: Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France.
authors.forename: Bertille
authors.initials: B
authors.lastname: de Barbeyrac
mesh_terms: D002163:Cameroon; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics*; D052776:Female Urogenital Diseases / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D052801:Male Urogenital Diseases; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis