title: Treatment of chronic hepatitis B virus infection via oral immune regulation toward hepatitis B virus proteins.
abstract: OBJECTIVE
  Hepatitis B virus (HBV) is a noncytopathic virus, and hepatocellular injury is mediated by a defective host antiviral immune response. We have previously shown that antiviral immunity can be modulated through oral feeding of viral proteins. The aims of this study were to determine the safety and efficacy of treatment of patients with chronic HBV by means of p.o. administration of HBV envelope proteins.
  
  
  METHODS
  A total of 42 chronic HBV patients were treated p.o. with HBV envelope proteins (HBsAg+preS1+preS2), three times/wk for 20-30 wk, and followed for an additional 20 wk. Patients were monitored for HBV-DNA levels, liver enzymes, and liver histology. HBV-directed T cell immune modulation was assessed in vitro by HBV specific T cell-proliferation, cytotoxicity, IFN gamma, and IL10 ELISPOT assays, and reverse transcription-polymerase chain reaction cytokines assay.
  
  
  RESULTS
  Favorable response in one of the primary endpoints was achieved in 28/42 patients (66.6%) by means of p.o. immune regulation. A significant decrease in viral load was observed in 15 patients (35.7%). HBsAg/HBcAg biopsy scores improved in 41% and 57.1% of patients, respectively. Histological improvement in liver necroinflammatory score was noted in 12/40 patients (30%). In all, 80% showed biochemical response. Five of 19 HBeAg positive patients (26.3%) became negative for HBeAg. A favorable augmentation in anti-HBV specific T cell response, with increased HbsAg specific T cell proliferation (78%), cytotoxicity (75%), and IFN gamma positive T cell clones (62.9%) was noted. In addition, a decrease in the IL10 gamma positive T cell clones was achieved (48.1%). Natural killer T (NKT) lymphocytes increased significantly in all treated patients.
  
  
  CONCLUSIONS
  Immune regulation of the anti-HBV immune response via p.o. administration of HBV envelope proteins alleviated the immune-mediated liver injury while augmenting the effective antiviral immunity.
authors.affiliation: Liver Unit, Department of Medicine, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: Rifaat
authors.initials: R
authors.lastname: Safadi
authors.forename: Eran
authors.initials: E
authors.lastname: Israeli
authors.forename: Orit
authors.initials: O
authors.lastname: Papo
authors.forename: Oren
authors.initials: O
authors.lastname: Shibolet
authors.forename: Alaa
authors.initials: A
authors.lastname: Melhem
authors.forename: Aharon
authors.initials: A
authors.lastname: Bloch
authors.forename: Mina
authors.initials: M
authors.lastname: Rowe
authors.forename: Ruslana
authors.initials: R
authors.lastname: Alper
authors.forename: Athalia
authors.initials: A
authors.lastname: Klein
authors.forename: Nilla
authors.initials: N
authors.lastname: Hemed
authors.forename: Ori
authors.initials: O
authors.lastname: Segol
authors.forename: Barbara
authors.initials: B
authors.lastname: Thalenfeld
authors.forename: Dean
authors.initials: D
authors.lastname: Engelhardt
authors.forename: Elazar
authors.initials: E
authors.lastname: Rabbani
authors.forename: Yaron
authors.initials: Y
authors.lastname: Ilan
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000368:Aged; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D005500:Follow-Up Studies; D006514:Hepatitis B Surface Antigens / Q000627:therapeutic use*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000628:therapy*; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology*; D007167:Immunotherapy / Q000379:methods; D008297:Male; D008875:Middle Aged; D011336:Probability; D011446:Prospective Studies; D018570:Risk Assessment; D018709:Statistics, Nonparametric; D016896:Treatment Outcome; D014758:Viral Core Proteins / Q000187:drug effects*
chemical_list: D006514:Hepatitis B Surface Antigens; D014758:Viral Core Proteins