Substrate specificity for catalysis of phosphodiester cleavage by a dinuclear Zn(II) complex.
The 2.4 kcal mol(-1) greater stabilization of the transition state for cleavage of the minimal substrate HpPNP compared to the nucleoside substrate UpPNP by the efficient dinuclear metal ion catalyst Zn2(L2O) provides evidence that access to the cationic core of Zn2(L2O) is sterically blocked for the bulkier nucleoside substrates, a flaw that will need to be dealt with in later generations of metal ion catalysts of RNA cleavage.
Yang
Meng-Yin
MY
Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260-3000, USA.
Richard
John P
JP
Morrow
Janet R
JR
D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D010755:Organophosphates / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D015032:Zinc / Q000737:chemistry*
D010755:Organophosphates; D012313:RNA; D015032:Zinc