title: Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry.
abstract: The formal reduction potential (Eo') of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (+/-0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant.
authors.affiliation: Department of Chemistry, Birmingham Southern College, Birmingham, AL 35254, USA. sdorman@bsc.edu
authors.forename: Scott C
authors.initials: SC
authors.lastname: Dorman
authors.forename: John P
authors.initials: JP
authors.lastname: Harrington
authors.forename: Melinda S
authors.initials: MS
authors.lastname: Martin
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Johnson
mesh_terms: D000818:Animals; D004563:Electrochemistry / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry*; D006736:Horses; D009211:Myoglobin / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods
chemical_list: D006454:Hemoglobins; D009211:Myoglobin