[Immunohistochemical investigation of lungs with severe acute respiratory syndrome].
OBJECTIVE
To investigate the roles of different cells in the pulmonary lesions in the severe acute respiratory syndrome (SARS) patients.


METHODS
The monoclonal antibodies of CD8, CD20, CD34, LCA, CD56, CD68, and AE1/AE3 are used to demonstrate the different cells in the lung specimens of SARS patients in order to study the patterns of cell responses in this new disease. Meanwhile the HE stained slides were also carefully studied to compare with the results of immunohistochemical staining.


RESULTS
The number of capillaries increased and the capillaries clearly outlined the contour of alveolar wall from beginning to early stage of organization, the number of lymphocytes decreased sharply while the number of macrophage remarkably increased, together with proliferation of type II pneumocytes. The numbers of blood vessels decreased in the fibrotic and consolidated lung tissue, and the vessel cavities enlarged, losing the normal contour of alveolar septa.


CONCLUSIONS
The lesions in the lung from SARS patients are consisted of the tissue reaction to the inflammatory injury, including extensive exudation, capillary proliferation, fibrosis, and obvious infiltration of macrophages which may play a key role in the pathogenesis of pulmonary lesions of SARS.
Lu
Zhao-hui
ZH
Department of Pathology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Chen
Jie
J
Luo
Yu-feng
YF
Cao
Jin-ling
JL
Wan
Jian-wei
JW
Wang
De-tian
DT
Zhang
Hong-tu
HT
Xie
Yong-qiang
YQ
D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D018951:Antigens, CD20 / Q000276:immunology; D018952:Antigens, CD34 / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D002196:Capillaries / Q000473:pathology; D004487:Edema / Q000473:pathology; D005260:Female; D005355:Fibrosis / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000473:pathology; D045169:Severe Acute Respiratory Syndrome / Q000473:pathology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D018951:Antigens, CD20; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule