title: Analysis of HLA class I and II alleles in sporadic inclusion-body myositis.
abstract: Sporadic inclusion body myositis (s-IBM) is characterised by progressive weakness of proximal and distal limb muscles. Most patients are aged over 50 years at disease onset. Muscle biopsy reveals an inflammatory myopathy and cytoplasmic amyloid deposits. The mononuclear infiltrate is dominated by CD8+ T-cells. Several investigators have described associations between s-IBM and certain HLA antigens and alleles. However, to date neither HLA class I nor II alleles have been analysed in a large series of patients. We typed various HLA class I and II alleles in 47 patients suffering from s-IBM using sequence specific-primer pairs (SSPPCR). The results were compared with published German controls. Additional Bonferroni adjustment was performed over all allele groups corresponding to serologically defined antigens within one HLA class I or II locus. After Bonferroni adjustment, we found a significant increase in frequency of the following HLA alleles for s-IBM patients when compared with normal controls: A*03 (p = 0.0002), B*08 (p = 0.002), DRB1*03 (p = 0.0000002), and DQB1*05 (p = 0.02). HLA typing may be helpful to distinguish between subgroups of s-IBM patients. Moreover, HLA analysis may aid in identifying patients who might profit from future therapeutic strategies.
authors.affiliation: Klinik für Neurologie, Technische Universität Dresden, Fetscherstrasse 74, 01307, Dresden, Germany. johannes.lampe@schering.de
authors.forename: Johannes B
authors.initials: JB
authors.lastname: Lampe
authors.forename: Gudrun
authors.initials: G
authors.lastname: Gossrau
authors.forename: Andrea
authors.initials: A
authors.lastname: Kempe
authors.forename: Monika
authors.initials: M
authors.lastname: Füssel
authors.forename: Katja
authors.initials: K
authors.lastname: Schwurack
authors.forename: Rolf
authors.initials: R
authors.lastname: Schröder
authors.forename: Sabine
authors.initials: S
authors.lastname: Krause
authors.forename: Ralf
authors.initials: R
authors.lastname: Kohnen
authors.forename: Maggie C
authors.initials: MC
authors.lastname: Walter
authors.forename: Heinz
authors.initials: H
authors.lastname: Reichmann
authors.forename: Hanns
authors.initials: H
authors.lastname: Lochmüller
mesh_terms: D000483:Alleles; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D018979:Myositis, Inclusion Body / Q000235:genetics*; D016133:Polymerase Chain Reaction