Biogeochemical processes and microbial characteristics across groundwater-surface water boundaries of the Hanford Reach of the Columbia River.
Biogeochemical processes within riverbed hyporheic zones (HZ) can potentially impact the fate and transport of contaminants. We evaluated a modified freeze core technique for the collection of intact cobble-bed samples from the Columbia River HZ along a stretch of the Hanford Reach in Washington State and investigated microbiological and geochemical parameters of corresponding frozen and unfrozen samples. During three sampling periods (March, May, and November 2000), relatively high numbers of viable aerobic heterotrophic bacteria were recovered from both unfrozen (10(6)-10(7) cfu/g) and frozen samples (10(5)-10(6) cfu/g). Relatively large populations of sulfate-, nitrate-, and iron-reducing bacteria were present, and significant concentrations of acid-volatile sulfide were measured in some samples, indicating that anoxic regions exist within this zone. Cr(VI), a priority groundwater pollutant on adjacent U.S. Department of Energy lands, was probably removed from solution in HZ samples by a combination of microbial activity and chemical reduction, presumably via products of anaerobic microbial metabolism. These results suggest that biogeochemical processes in the Columbia River HZ may contribute to the natural attenuation of Cr(VI). Although freezing modestly diminished recovery of viable bacteria, freeze core techniques proved reliable for the collection of intact hyporheic sediments.
Moser
Duane P
DP
Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. duane.moser@pnl.gov
Fredrickson
James K
JK
Geist
David R
DR
Arntzen
Evan V
EV
Peacock
Aaron D
AD
Li
Shu-Mei W
SM
Spadoni
Tina
T
McKinley
James P
JP
D001673:Biodegradation, Environmental; D004784:Environmental Monitoring / Q000379:methods*; D005615:Freezing; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D016062:Porosity; D012988:Soil Microbiology; D013048:Specimen Handling; D014861:Washington; D014871:Water Microbiology; D014872:Water Movements; D014874:Water Pollutants, Chemical / Q000032:analysis*
D014874:Water Pollutants, Chemical