Angiotensin-converting enzyme (CD143) is abundantly expressed by dendritic cells and discriminates human monocyte-derived dendritic cells from acute myeloid leukemia-derived dendritic cells.
OBJECTIVE
The pattern of angiotensin-converting enzyme (ACE) expression in dendritic cells (DC) and macrophages derived from normal monocytes vs that in DC derived from acute myeloid leukemia blasts was investigated.


METHODS
ACE expression was quantified by flow cytometry using a set of monoclonal antibodies (mAbs) directed against five different epitopes on the ACE molecule and by enzyme activity measurement.


RESULTS
The binding pattern of a set of anti-ACE mAbs to the surface of blood cells and their progeny, as revealed by FACS, showed lineage and epitope specificity. Differentiation of monocytes to macrophages and DC was accompanied by a dramatic increase in ACE expression. ACE activity was 50-fold higher in macrophages and 150-fold higher in DC than in monocytes. ACE level normalized per cell revealed that DC expressed 1300-fold more ACE than did monocytes. In contrast, DC derived from acute myeloid leukemia blasts did not show an elevated level of ACE, although they acquired DC markers CD80, CD40, and CD86 upon cytokine or calcium ionophore treatment.


CONCLUSIONS
ACE expression becomes the first marker to functionally distinguish DC generated from monocytes and leukemic blast cells. Given that ACE plays an important role in the hydrolysis of many peptides, as well as in the presentation of some antigens to immune cells, these data suggest that elevated ACE expression on the surface of DC is not just a reflection of the general activation of monocytes during differentiation; rather, it may be physiologically important for the functioning of these cells.
Danilov
Sergei M
SM
Department of Anesthesiology, Anesthesiology Research Center, University of Illinois at Chicago, 1819 W. Polk Street (M/C 519), Chicago, IL 60612, USA. danilov@uic.edu
Sadovnikova
Elena
E
Scharenborg
Nicole
N
Balyasnikova
Irina V
IV
Svinareva
Daria A
DA
Semikina
Elena L
EL
Parovichnikova
Elena N
EN
Savchenko
Valery G
VG
Adema
Gosse J
GJ
D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis; D001773:Blood Cells; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000201:enzymology*; D005434:Flow Cytometry; D006801:Humans; D007951:Leukemia, Myeloid / Q000201:enzymology / Q000473:pathology*; D009000:Monocytes / Q000166:cytology*; D007703:Peptidyl-Dipeptidase A / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology
D015703:Antigens, CD; D007703:Peptidyl-Dipeptidase A