Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics.
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.
Tolstikov
Vladimir V
VV
Department Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany.
Lommen
Arjen
A
Nakanishi
Kazuki
K
Tanaka
Nobuo
N
Fiehn
Oliver
O
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D010936:Plant Extracts / Q000032:analysis; D012822:Silicon Dioxide; D012984:Software; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013057:Spectrum Analysis
D010936:Plant Extracts; D012822:Silicon Dioxide