Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.
AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription.
Wozniak
Daniel J
DJ
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. dwozniak@wfubmc.edu
Sprinkle
April B
AB
Baynham
Patricia J
PJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
C079619:AlgU protein, Pseudomonas aeruginosa; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D012808:Sigma Factor