title: Contact deformation of liposome in the presence of osmosis.
abstract: The role of osmotic pressure on the geometry of adherent liposome remains an intricate question in the mechanics of supramolecular structures. In this study, confocal reflection interference contrast microscopy in combination with cross-polarized microscopy was applied to probe the geometry of deformed liposome on fused silica substrates through the determination of a vesicle-substrate separation profile. In parallel, a theoretical model which describes the large deformation of the lipid bilayer membrane under both out-plane bending and in-plane shear forces is developed. Then, the global deformation geometry of the adherent liposome is rigorously compared with our experimental data. It is shown that the adhesion contact area increases in dimension, the liposome volume decreases, and the vesicle height decreases under the reduced osmotic pressure. The coupling of experimental data and a modified theoretical framework of the adherent liposome provides a more explicit result in comparison with previous studies and demonstrates the possibility of modeling the change of liposome mechanics under the influence of osmosis.
authors.affiliation: Tissue Engineering Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, Singapore 639798.
authors.forename: Ji-Jinn
authors.initials: JJ
authors.lastname: Foo
authors.forename: Vincent
authors.initials: V
authors.lastname: Chan
authors.forename: Kuo-Kang
authors.initials: KK
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D003198:Computer Simulation; D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D046911:Macromolecular Substances; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008956:Models, Chemical; D008968:Molecular Conformation; D009038:Motion; D009995:Osmosis; D009997:Osmotic Pressure; D010713:Phosphatidylcholines / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D046911:Macromolecular Substances; D010713:Phosphatidylcholines; C010942:1,2-distearoyllecithin