title: Use of recombinant cyprosin in the manufacture of ewe's milk cheese.
abstract: A recombinant cyprosin from the cardoon (Cynara cardunculus L.) was assayed and compared with calf rennet in batches of ewes' milk cheese by determining different chemical, biochemical, and microbiological parameters over 4 months of ripening. There were no differences between the two types of coagulants in most chemical parameters, a(w), and pH. Proteolysis was more marked and rapid in cheese containing recombinant cyprosin as coagulant, the soluble nitrogen content of which was significantly higher (p < 0.01) than that of the cheese obtained with animal rennet; at the end of ripening the nonprotein nitrogen of cheese produced with recombinant cyprosin was slightly higher (p > 0.05) as compared with that in cheeses obtained with animal rennet. Microbial counts in the milk used for making cheese were high in most of the groups analyzed. Despite slight differences in counts, the main microbial groups analyzed were similar in cheese produced with both types of coagulants throughout ripening.
authors.affiliation: Departamento de Bromatología y Tecnología de los Alimentos, Campus de Rabanales, Edificio C-1, Universidad de Córdoba, E-14014 Córdoba, Spain. ao l fecaj@lucano.uco.es
authors.forename: José
authors.initials: J
authors.lastname: Fernández-Salguero
authors.forename: Francisco
authors.initials: F
authors.lastname: Prados
authors.forename: Filomena
authors.initials: F
authors.lastname: Calixto
authors.forename: Montserrat
authors.initials: M
authors.lastname: Vioque
authors.forename: Pedro
authors.initials: P
authors.lastname: Sampaio
authors.forename: Luis
authors.initials: L
authors.lastname: Tejada
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases; D002417:Cattle; D002611:Cheese / Q000382:microbiology; D012085:Chymosin; D031942:Cynara / Q000201:enzymology; D005260:Female; D005511:Food Handling / Q000379:methods*; D008892:Milk / Q000382:microbiology; D011994:Recombinant Proteins; D012756:Sheep
chemical_list: D011994:Recombinant Proteins; C025350:rennet; D016282:Aspartic Acid Endopeptidases; C087364:cyprosin; D012085:Chymosin