title: Evidence for heme oxygenase-1 association with caveolin-1 and -2 in mouse mesangial cells.
abstract: The interaction of heme oxygenase-1 (HO-1) and caveolin in the cultured mouse mesangial cells (MMC) was investigated. In normal MMCs, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Upon treating the MMCs either with cadmium (Cd) or spermine NONOate (SPER/NO), expression of HO-1 mRNA and protein was increased. Caveolae rich membranous fractions from the MMCs treated with Cd or SPER/NO contained both HO-1 and caveolin-1 or caveolin-2. The experiments of immuno-precipitation showed complex formation between the HO-1 and caveolin-1 or caveolin-2 in the Cd treated MMCs. Confocal microscopic results also support co-localization of HO-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with HO-1 in caveolae suggested that caveolin could also play an important role in regulating the function of HO-1.
authors.affiliation: Department of Pharmacology and Toxicology, College of Medicine, Inha University, Incheon, Korea.
authors.forename: Nam-Hee
authors.initials: NH
authors.lastname: Jung
authors.forename: Hong Pyo
authors.initials: HP
authors.lastname: Kim
authors.forename: Bum-Rae
authors.initials: BR
authors.lastname: Kim
authors.forename: Seok Ho
authors.initials: SH
authors.lastname: Cha
authors.forename: Gyeong A
authors.initials: GA
authors.lastname: Kim
authors.forename: Hunjoo
authors.initials: H
authors.lastname: Ha
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Na
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Cha
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000187:drug effects / Q000378:metabolism*; D011232:Chemical Precipitation; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000187:drug effects / Q000378:metabolism*; D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D051379:Mice; D018613:Microscopy, Confocal; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009589:Nitrogen Oxides; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D008565:Membrane Proteins; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D002104:Cadmium; C091861:spermine nitric oxide complex; D013096:Spermine; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse