title: CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm.
abstract: CatSper family proteins are putative ion channels expressed exclusively in membranes of the sperm flagellum and required for male fertility. Here, we show that mouse CatSper1 is essential for depolarization-evoked Ca2+ entry and for hyperactivated movement, a key flagellar function. CatSper1 is not needed for other developmental landmarks, including regional distributions of CaV1.2, CaV2.2, and CaV2.3 ion channel proteins, the cAMP-mediated activation of motility by HCO3-, and the protein phosphorylation cascade of sperm capacitation. We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization.
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carlson
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Westenbroek
authors.forename: Timothy
authors.initials: T
authors.lastname: Quill
authors.forename: Dejian
authors.initials: D
authors.lastname: Ren
authors.forename: David E
authors.initials: DE
authors.lastname: Clapham
authors.forename: Bertil
authors.initials: B
authors.lastname: Hille
authors.forename: David L
authors.initials: DL
authors.lastname: Garbers
authors.forename: Donner F
authors.initials: DF
authors.lastname: Babcock
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000502:physiology*; D020746:Calcium Channels, L-Type / Q000737:chemistry; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D000242:Cyclic AMP / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007477:Ions; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D009154:Mutation; D010766:Phosphorylation; D013094:Spermatozoa / Q000378:metabolism*; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism
chemical_list: D001639:Bicarbonates; C438823:CATSPER1 protein, human; C498916:Cacna1e protein, mouse; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D007477:Ions; C419385:L-type calcium channel alpha(1C); D014443:Tyrosine; D000242:Cyclic AMP; D002118:Calcium