Interleukin-1beta gene polymorphisms in Taiwanese patients with gout.
The purpose of this study was to examine whether interleukin-1 beta (IL-1beta) promoter and exon 5 gene polymorphisms are markers of susceptibility or clinical manifestations in Taiwanese patients with gout. The study included 196 patients in addition to 103 unrelated healthy control subjects living in central Taiwan. From genomic DNA, polymorphisms of the gene for IL-1beta promoter and IL-1beta exon 5 were typed. Allelic frequencies were compared between the two groups, and the relationship between allelic frequencies and clinical manifestations of gout was evaluated. No significant differences were observed in the allelic frequencies of the IL-1beta promoter between patients with gout and healthy control subjects. Additionally, we did not detect any association of the IL-1beta promoter genotype with the clinical and laboratory profiles of gout patients. However, there was a significant difference between the two groups in terms of hypertriglyceridemia (P=0.0004, chi(2)=12.52, OR 7.14, 95%CI 0.012-0.22). There was also a significant difference in the genotype of IL-1beta exon 5 polymorphism between patients with and without hypertriglyceridemia. Results of the present study suggest that polymorphisms of the IL-1beta promoter and IL-1beta exon 5 are not related to gout patients in central Taiwan.
Chen
Man-Ling
ML
Department of Medical Genetics, China Medical University Hospital, College of Chinese Medicine, China Medical University, Taichung, Taiwan.
Huang
Chung-Ming
CM
Tsai
Chang-Hai
CH
Tsai
Fuu-Jen
FJ
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D016001:Confidence Intervals; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006073:Gout / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D013624:Taiwan
D005819:Genetic Markers; D007375:Interleukin-1