title: LC/ESI-MS analysis of two elastin cross-links, desmosine and isodesmosine, and their radiation-induced degradation products.
abstract: In this work, the effect of Fenton reaction on two elastin cross-linked amino acids, desmosine (DES) and isodesmosine (IDE), in the absence or presence of different wavelength radiations generated from artificial sources has been evaluated using LC/ESI-MS. Irradiation as well as incubation of DES or IDE solutions in the presence of Fe(2+) and H(2)O(2) resulted in products with m/z 497.1 and 481.1 for [M+H](+). A strongly dose-dependent degradation of both amino acids was observed upon exposure to UVB at doses ranging from 0 to 3 J/cm(2) and a moderate dose-dependent degradation upon exposure to UVA at doses 10 times higher than that of UVB. A significant time-dependent degradation of DES and IDE was also observed upon exposure of these amino acids to a lamp emitting visible light similar to sunlight. Exposure of both amino acids to IR radiation (520 W) for 8 h did not cause significant degradation.
authors.affiliation: Institute of Pharmaceutics and Biopharmaceutics, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck Str. 4, D-06120, Halle(Saale), Germany.
authors.forename: Melkamu
authors.initials: M
authors.lastname: Getie
authors.forename: Klaus
authors.initials: K
authors.lastname: Raith
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
mesh_terms: D002853:Chromatography, Liquid; D003895:Desmosine / Q000032:analysis* / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D007524:Isodesmosine / Q000032:analysis* / Q000737:chemistry / Q000528:radiation effects; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays
chemical_list: D003895:Desmosine; D007524:Isodesmosine