title: Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
abstract: Monocytes-macrophages, the target cells of African swine fever virus (ASFV) are highly heterogeneous in phenotype and function. In this study, we have investigated the correlation between the phenotype of specific populations of porcine macrophages and their permissiveness to ASFV infection. Bone marrow cells and fresh blood monocytes were less susceptible to in vitro infection by ASFV than more mature cells, such as alveolar macrophages. FACS analyses of monocytes using a panel of mAbs specific for porcine monocyte/macrophages showed that infected cells had a more mature phenotype, expressing higher levels of several macrophage specific markers and SLA II antigens. Maturation of monocytes led to an increase in the percentage of infected cells, which correlated with an enhanced expression of CD163. Separation of CD163+ and CD163- monocytes demonstrated the specific sensitivity of the CD163+ subset to ASFV infection. In vivo experiments also showed a close correlation between CD163 expression and virus infection. Finally, mAb 2A10 and, in a lower extent, mAb 4E9 were able to inhibit, in a dose-dependent manner, both ASFV infection and viral particle binding to alveolar macrophages. Altogether, these results strongly suggest a role of CD163 in the process of infection of porcine monocytes/macrophages by ASFV.
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña km. 7.5, 28040 Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Sánchez-Torres
authors.forename: P
authors.initials: P
authors.lastname: Gómez-Puertas
authors.forename: M
authors.initials: M
authors.lastname: Gómez-del-Moral
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: J M
authors.initials: JM
authors.lastname: Escribano
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000502:physiology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis / Q000502:physiology*; D025701:Asfarviridae / Q000502:physiology*; D002478:Cells, Cultured; D008264:Macrophages / Q000737:chemistry / Q000821:virology*; D009000:Monocytes / Q000737:chemistry / Q000821:virology*; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000032:analysis / Q000502:physiology*; D013552:Swine; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface