Expression and characterization of the C345C/NTR domains of complement components C3 and C5.
Complement components C3, C4, and C5 are members of the thioester-containing alpha-macroglobulin protein superfamily. Within this superfamily, a unique feature of the complement proteins is a 150-residue-long C-terminal extension of their alpha-subunits that harbors three internal disulfide bonds. Previous reports have suggested that this is an independent structural module, homologous to modules found in other proteins, including netrins and tissue inhibitors of metalloproteinases. Because of its distribution, this putative module has been named both C345C and NTR. To assess the structures of these segments of the complement proteins, their relationships with other domains, and activities as independent structures, we expressed C345C from C3 and C5 in a bacterial strain that permits cytoplasmic disulfide bond formation. Affinity purification directly from cell lysates yielded recombinant C3- and C5-C345C with properties consistent with multiple intramolecular disulfide bonds and high beta-sheet contents. rC5-, but not rC3-C345C inhibited complement hemolytic activity, and surface plasmon resonance studies revealed that rC5-C345C binds to complement components C6 and C7 with dissociation constants of 10 and 3 nM, respectively. Our results provide strong evidence that this binding corresponds to the previously described reversible binding of C5 to C6 and C7, and taken together with earlier work, indicate that the C5-C345C module interacts directly with the factor I modules in C6 and C7. The high binding affinities suggest that complexes composed of C5 bound to C6 or C7 exist in plasma before activation and may facilitate assembly of the complement membrane attack complex.
Thai
Chuong-Thu
CT
Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA.
Ogata
Ronald T
RT
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D001667:Binding, Competitive / Q000276:immunology; D003176:Complement C3 / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D003182:Complement C5 / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003183:Complement C6 / Q000378:metabolism; D003184:Complement C7 / Q000378:metabolism; D015941:Complement Hemolytic Activity Assay / Q000379:methods; D003169:Complement Inactivator Proteins / Q000096:biosynthesis / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D000075386:Netrin Receptors; D010957:Plasmids; D011485:Protein Binding / Q000276:immunology; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000235:genetics; D021122:Protein Subunits / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
D003176:Complement C3; D003182:Complement C5; D003183:Complement C6; D003184:Complement C7; D003169:Complement Inactivator Proteins; D004220:Disulfides; D000075386:Netrin Receptors; D021122:Protein Subunits; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D003545:Cysteine