title: Cilostazol reduces brain lesion induced by focal cerebral ischemia in rats--an MRI study.
abstract: To investigate the effects of cilostazol on the hemispheric ischemic lesion, we monitored the apparent diffusion coefficient (ADC) and T2 images by MRI techniques in comparison with histology at the terminal of and after 24-h reperfusion following 2-h occlusion of middle cerebral artery (MCA). The ADC values of tissue water and T2-weighted images were quantified by high field magnetic resonance. No significant difference was observed by ADC image among vehicle and cilostazol treatment groups when measured during MCA occlusion. Oral treatment with cilostazol 30 mg/kg two times at 5 min and 4 h significantly suppressed the hemispheric lesion area and volumes when detected by ADC, T2 images and histology, but 3 and 10 mg/kg cilostazol were without effect. Cilostazol (30 mg/kg) significantly reduced the increased cerebral water content at the ischemic hemisphere compared with vehicle group. In line with these results, the neurological deteriorations were much improved in the cilostazol-treated group. Taken together, it is concluded that post-treatment with cilostazol exerts a potent protective effect against cerebral infarct size by reducing the cytotoxic edema.
authors.affiliation: Department of Pharmacology, College of Medicine, Busan National University, Ami-Dong 1-Ga, Seo-Gu, Pusan 602-739, South Korea.
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yong-Kyu
authors.initials: YK
authors.lastname: Lee
authors.forename: Makoto
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Koga
authors.forename: Mari
authors.initials: M
authors.lastname: Fukunaga
authors.forename: Goro
authors.initials: G
authors.lastname: Miyakoda
authors.forename: Toyoki
authors.initials: T
authors.lastname: Mori
authors.forename: Tetsumi
authors.initials: T
authors.lastname: Hosokawa
authors.forename: Ki Whan
authors.initials: KW
authors.lastname: Hong
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D001929:Brain Edema / Q000188:drug therapy* / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D000077407:Cilostazol; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013777:Tetrazoles / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D013777:Tetrazoles; D000077407:Cilostazol