title: [Clinical significance of assessment of the metabolism of connective-tissue biopolymers in patients with type 1 diabetes mellitus and with diabetic nephropathy].
abstract: The purpose of the case study was to define a degree of destructive changes in the connective tissue at different stages of diabetic nephropathy (DN) progression. One hundred and twenty eight DN patients with type 1 diabetes mellitus, disease duration-5 to 20 years (classification according C. Mogensen, 1983) were examined. Special biochemical tools were used to evaluate the connective-tissue condition. The parameters of metabolism of the connective-tissue biopolymers were investigated in blood, urea and saliva, i.e. general and sulphated glucosamine glycine (GAG), sialic acid (SA) and oxyproline fractions. The results showed an increasing value of total and sulphated GAG and SA with a worsening DN severity, which is indicative of a destruction degree of glycoprotein-complexes in the microvascular basal membranes. A high level of bound oxyproline fractions denotes the fibril-genesis processes, which start to activate yet at the preclinical DN stage. The changes detected previously in the parameters of metabolism of the connective-tissue biopolymers, as observed in DN patients, ensure a timely choice of a therapy and a proper monitoring of its efficiency.
authors.forename: I A
authors.initials: IA
authors.lastname: Kazakova
authors.forename: V V
authors.initials: VV
authors.lastname: Trusov
authors.forename: I B
authors.initials: IB
authors.lastname: Cheremiskina
mesh_terms: D000328:Adult; D001704:Biopolymers / Q000097:blood / Q000378:metabolism* / Q000652:urine; D003238:Connective Tissue / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000209:etiology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000209:etiology / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism
chemical_list: D001704:Biopolymers