Improvement of protein crystal quality by forced flow solution.
Flow experiments in growing protein crystals were conducted to clarify the influence of the solution flow on the crystal quality. Lysozyme crystals grown under various flow velocities were analyzed by using synchrotron radiation to assess the quality. As a result, the crystals grown under forced flow were of better quality than those grown in quiescent conditions.
Kadowaki
Akio
A
Tokyo Institute of Technology, Materials Interdisciplinary Graduate School of Science and Engineering, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan. kadowaki.akio@nasda.go.jp
Yoshizaki
Izumi
I
Rong
Long
L
Komatsu
Hiroshi
H
Odawara
Osamu
O
Yoda
Shinichi
S
D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D009113:Muramidase / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017356:Synchrotrons
D011506:Proteins; C421864:hen egg lysozyme; D009113:Muramidase