title: Characterization of vitreous B-cell infiltrates in patients with primary ocular lymphoma, using CDR3 size polymorphism analysis of antibody transcripts.
abstract: OBJECTIVE
  Histopathology usually cannot be performed and cytology is unfortunately frequently insufficient to confirm a suspicion of primary intraocular lymphoma (PIOL). The purpose of this study was to evaluate the Immunoscope technique for the identification of ocular B-cell monoclonal infiltrates in patients with malignant or immune conditions.
  
  
  METHODS
  Polymorphism analysis of the size of the third complementarity-determining region (CDR3) of heavy chain antibody transcripts was used to differentiate between a polyclonal infiltrate and a monoclonal infiltrate within a clinical vitreous sample of two groups of patients. PIOL was confirmed in all patients of the first group (n = 6). Five patients with autoimmune uveitis or immune-recovery uveitis associated with AIDS were included in the control group. The level of IL-10 in the vitreous was determined in all patients.
  
  
  RESULTS
  In five cases of severe PIOL, CDR3 polymorphism analysis confirmed the presence of a dominant B-cell clone within the eye. In one case of confirmed PIOL presenting with mild vitritis, CDR3 polymorphism analysis was consistent with the existence of a polyclonal profile in the ocular sample studied. Conversely, it was shown that the detection of an intraocular monoclonal B-cell population does not necessarily imply ocular lymphoma. A clonal expansion was detected in a control patient who exhibited merely a nonmalignant response associated with immune-recovery uveitis.
  
  
  CONCLUSIONS
  This PCR-based technique can make an important contribution to the characterization of intraocular B cells, but it alone cannot confirm or exclude the existence of a malignant lymphocyte proliferation. In the evaluation of a patient with intraocular inflammation in whom PIOL is suspected, CDR3 polymorphism analysis is recommended to confirm clonality. In general, the information about lymphocyte diversity provided by this technology opens up new possibilities for the analysis of ocular infiltrates.
authors.affiliation: Laboratory of Immunology A, National Institute for Health and Medical Research, Paris, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Gorochov
authors.forename: Christophe
authors.initials: C
authors.lastname: Parizot
authors.forename: Bahram
authors.initials: B
authors.lastname: Bodaghi
authors.forename: Darren
authors.initials: D
authors.lastname: Raphael
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cassoux
authors.forename: Hélène
authors.initials: H
authors.lastname: Merle-Beral
authors.forename: Patrice
authors.initials: P
authors.lastname: Debré
authors.forename: Phuc
authors.initials: P
authors.lastname: LeHoang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005134:Eye Neoplasms / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014821:Vitrectomy; D014822:Vitreous Body / Q000276:immunology* / Q000473:pathology
chemical_list: D022801:Complementarity Determining Regions; D004273:DNA, Neoplasm; D016753:Interleukin-10