title: Stable Bacillus thuringiensis (Bt) toxin content in interspecific F1 and backcross populations of wild Brassica rapa after Bt gene transfer.
abstract: Stable expression of a transgene may lead to increased fitness for wild plants after acquiring the transgene via crop-weed hybridization. Here, we investigate the stability of Bt toxin content in wild Brassica rapa acquiring the Bt gene from Bt Brassica napus. The Bt toxin content in nine Bt-expressing B. napus lines was 0.80-1.70 micro g/g leaf tissue throughout the growing season. These nine lines were crossed with three accessions of wild B. rapa and the Bt gene was successfully transferred to interspecific hybrids (F1) and successive backcross generations (BC1 to BC4). The Bt toxin level in F1 and BC progenies containing the Bt gene remained at 0.90-3.10 micro g/g leaf tissue. This study indicates that the Bt gene can persist and be stably expressed in wild B. rapa.
authors.affiliation: National Water Research Institute, Environment Canada, 11 Innovation Blvd, Saskatoon, Saskatchewan, S7N 3H5 Canada. bin.zhu@ec.gc.ca
authors.forename: B
authors.initials: B
authors.lastname: Zhu
authors.forename: J R
authors.initials: JR
authors.lastname: Lawrence
authors.forename: S I
authors.initials: SI
authors.lastname: Warwick
authors.forename: P
authors.initials: P
authors.lastname: Mason
authors.forename: L
authors.initials: L
authors.lastname: Braun
authors.forename: M D
authors.initials: MD
authors.lastname: Halfhill
authors.forename: C N
authors.initials: CN
authors.lastname: Stewart
mesh_terms: D001413:Bacillus thuringiensis / Q000737:chemistry*; D029688:Brassica napus / Q000737:chemistry / Q000235:genetics*; D029741:Brassica rapa / Q000737:chemistry / Q000235:genetics*; D003433:Crosses, Genetic; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006824:Hybridization, Genetic; D009864:Ontario; D019076:Transgenes / Q000235:genetics*