title: Preliminary study on rapid identification of Mycobacterium tuberculosis complex isolates by the BD ProbeTec ET system.
abstract: The BD ProbeTec ET system for identification of Mycobacterium tuberculosis complex (MTBC) isolates from BACTEC 12B culture vials was evaluated in comparison with BACTEC NAP (p-nitro-alpha-acetylamino-beta-hydroxy-propiophenone) differentiation. Of 145 mycobacterial isolates tested, comprising 89 MTBC and 56 non-tuberculous mycobacteria (NTM), BD ProbeTec ET correctly identified 87 MTBC and 56 NTM but missed two MTBC. Three NTM were misidentified when NAP was incubated at 37 degrees C only. Overall sensitivity, specificity and positive and negative predictive values were respectively 97.8, 100, 100 and 96.6 % for the BD ProbeTec ET system and 100, 94.6, 96.7 and 94.6 % for NAP.
authors.affiliation: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608.
authors.forename: S X
authors.initials: SX
authors.lastname: Wang
authors.affiliation: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608.
authors.forename: L H
authors.initials: LH
authors.lastname: Sng
authors.affiliation: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608.
authors.forename: L
authors.initials: L
authors.lastname: Tay
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology*