Conformational differences between native and recombinant horseradish peroxidase revealed by tritium planigraphy.
Significant conformational differences between native and recombinant horseradish peroxidase have been shown by tritium planigraphy, which includes a method of thermal activation of tritium followed by amino acid analysis of the protein preparation. Comparison of radioactivity distribution among the amino acid residues with the theoretical (calculated) accessibility shows that the recombinant enzyme is characterized by high hydrophobicity and compactness of folding. The protective role of oligosaccharides in native enzyme has been confirmed. An unexpected result of the study is a finding on high accessibility of a catalytic histidine residue in solution. An effect of low dose (3 Gy) of irradiation on the accessibility of amino acid residues has been unequivocally demonstrated. The data can be interpreted as swelling of the compact folding and increase in the surface hydrophilicity of the recombinant enzyme. In the case of native enzyme, irradiation does not cause remarkable changes in the accessibility of amino acid residues indicating the possible extensive radical modification of the native enzyme in the life-course of the cell. The catalytic histidine is an exception. It becomes inaccessible after the enzyme irradiation, while its accessibility in the recombinant enzyme increases. An additional observation of a 5-fold decrease in the rate constant towards hydrogen peroxide points to the destructive effect of irradiation on the hydrogen bond network in the distal domain of the native enzyme molecule and partial collapse of the active site pocket.
Orlova
M A
MA
Department of Radiochemistry, Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia. tchubar@enz.chem.msu.ru
Chubar
T A
TA
Fechina
V A
VA
Ignatenko
O V
OV
Badun
G A
GA
Ksenofontov
A L
AL
Uporov
I V
IV
Gazaryan
I G
IG
D000596:Amino Acids / Q000032:analysis; D004795:Enzyme Stability / Q000528:radiation effects; D005720:Gamma Rays; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D011487:Protein Conformation / Q000528:radiation effects; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects; D014316:Tritium
D000596:Amino Acids; D011994:Recombinant Proteins; D014316:Tritium; D006735:Horseradish Peroxidase