Evidence of a transcriptional co-activator function of cohesin STAG/SA/Scc3.
Cohesins hold sister chromatids together from DNA replication until they are segregated. Although cohesins Smc1, Smc3, and Scc1/Rad21 are involved in chromatid cohesion and other cellular processes, little is known about the other mitotic cohesin subunit, Scc3/STAG. Here we describe STAG/Scc3, which may act as a transcriptional co-activator. STAG2 is able to enhance the activity of the tumor necrosis factor alpha, the CD69, and the human immunodeficiency virus long terminal repeat promoters in a NF-kappaB-dependent manner. In addition, STAG2 interacts with the viral transactivator Tat and enhances the Tat-mediated activation of the human immunodeficiency virus long terminal repeat promoter. Moreover, STAG2 co-activates a multimeric NF-kappaB reporter construct and enhances the activity of the transactivation domain of p65/RelA in a Gal4 system. This function is dependent on one of the LXXLL co-activation motives present in this cohesin and is substantiated by the interaction of STAG2 with the p65 subunit of NF-kappaB. These results describe a novel activity for cohesins, suggesting a role for STAG/Scc3 in transcriptional regulation.
Lara-Pezzi
Enrique
E
Unidad de Biología Molecular, Hospital de la Princesa, Diego de León, 62, 28006 Madrid, Spain.
Pezzi
Nieves
N
Prieto
Ignacio
I
Barthelemy
Isabel
I
Carreiro
Candelas
C
Martínez
Alicia
A
Maldonado-Rodríguez
Alejandra
A
López-Cabrera
Manuel
M
Barbero
José Luis
JL
D020816:Amino Acid Motifs; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D018797:Cell Cycle Proteins; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D016325:HIV Long Terminal Repeat; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D037181:Lectins, C-Type; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013997:Time Factors; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000097722:Cohesins; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D037181:Lectins, C-Type; D016328:NF-kappa B; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D000097722:Cohesins; D000099192:CD69 Antigens; C052130:GAL4 protein, S cerevisiae; C476538:Stag2 protein, mouse