Effect of different matrices on physiological amino acids analysis by liquid chromatography: evaluation and correction of the matrix effect.
Free amino acids in plasma, muscle and liver tissues were determined using the Pico-Tag method which involves HPLC and pre-column derivatization. A study of the accuracy in the determination is carried out by means of external calibration, standard-added calibration, Youden calibration and analyte recovery. It is conclusively shown that the method is affected by a proportional systematic error due to matrix effects, but not by a constant one. A new function named matrix-corrected calibration is proposed to correct for proportional bias when free analyte matrices for matrix-matched addition is unavailable.
Fernández-Fígares
Ignacio
I
Estacion Experimental del Zaidin, Spanish Council for Scientific Research (CSIC), Department of Animal Nutrition, Camino del Jueves s/n, 1100 Armilla, Granada, Spain
Rodríguez
Luis Cuadros
LC
González-Casado
Antonio
A
D000596:Amino Acids / Q000032:analysis* / Q000097:blood; D000818:Animals; D002138:Calibration; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*
D000596:Amino Acids