[Relationship of apoptosis and expression of Fas gene in tongue squamous cell carcinoma cell line Tca8113].
OBJECTIVE
To investigate the relationship of apoptosis and expression of Fas gene in oral squamous cell carcinoma.


METHODS
Plasmid including Fas gene was transfected into Tca8113 cell by lipofectamine kit. The expression of Fas mRNA in transfected and non-transfected cell was detected by RT-PCR. The expression of Fas protein and apoptosis index(AI) was assessed by flow cytometry(FCM).


RESULTS
No variation of positive expression rate of Fas protein was found in Fas-transfected and non-transfected Tca8113 (P > 0.05), but the expression of Fas mRNA, intensity of Fas protein and apoptosis index were significantly increased respectively from 0.201 +/- 0.015, 31.02 +/- 4.63, 0.88% +/- 0.16% to 0.399 +/- 0.073, 54.32 +/- 6.38 and 10.21% +/- 1.46%(P < 0.01).


CONCLUSIONS
Apoptosis in tongue squamous cell carcinoma was not directly associated with the positive expression rate but intensity of Fas protein. A liminal value might exist before apoptosis in Tca8113 cell could be activated.
Huang
Hong-zhang
HZ
Department of Oral and Maxillofacial Surgery, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
Hou
Jin-song
JS
Wang
Jian-guang
JG
Cheng
Bin
B
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000235:genetics*
D012333:RNA, Messenger; D019014:fas Receptor