title: PRELI (protein of relevant evolutionary and lymphoid interest) is located within an evolutionarily conserved gene cluster on chromosome 5q34-q35 and encodes a novel mitochondrial protein.
abstract: The characterization of mitochondrial proteins is important for the understanding of both normal cellular function and mitochondrial disease. In the present study we identify a novel mitochondrial protein, PRELI (protein of relevant evolutionary and lymphoid interest), that is encoded within the evolutionarily conserved MAD3/PRELI/RAB24 gene cluster located at chromosome 5q34-q35. Mouse Preli is expressed at high levels in all settings analysed; it is co-expressed with Rab24 from a strong bi-directional promoter, and is regulated independently from the S-phase-specific Mad3 gene located at its 3' end. PRELI contains a stand-alone 170 amino acid PRELI/MSF1p' motif at its N-terminus. This domain is found in a variety of proteins from diverse eukaryotes including yeast, Drosophila and mammals, but its function is unknown, and the subcellular location of higher eukaryotic PRELI/MSF1P' proteins has not been determined previously. We show here that PRELI is located in the mitochondria, and by using green-fluorescent-protein fusion proteins we identify a mitochondrial targeting signal at its N-terminus.
authors.affiliation: School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Fox
authors.forename: Sally A
authors.initials: SA
authors.lastname: Stubbs
authors.forename: Jimmy
authors.initials: J
authors.lastname: Kyaw Tun
authors.forename: Jack P
authors.initials: JP
authors.lastname: Leek
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Markham
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Wright
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D012097:Repressor Proteins / Q000235:genetics; D020691:rab GTP-Binding Proteins / Q000235:genetics
chemical_list: D024101:Mitochondrial Proteins; C472089:Mxd3 protein, mouse; C487290:PRELI protein, mouse; D021382:Protein Sorting Signals; D012097:Repressor Proteins; C086224:Rab24 protein, mouse; D020691:rab GTP-Binding Proteins