Thermodynamic treatment of oligonucleotide duplex-simplex equilibria.
Thermodynamic formulations have been devised to obtain Delta G degrees values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.
Owczarzy
Richard
R
Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA.
Dunietz
Isard
I
Behlke
Mark A
MA
Klotz
Irving M
IM
Walder
Joseph A
JA
D001622:Betaine / Q000737:chemistry; D001669:Biochemical Phenomena; D001671:Biochemistry; D007477:Ions; D007700:Kinetics; D008962:Models, Theoretical; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012964:Sodium / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics
D007477:Ions; D009841:Oligonucleotides; D000644:Quaternary Ammonium Compounds; D001622:Betaine; D012964:Sodium