Anti-alpha-fodrin autoantibodies in Moyamoya disease.
OBJECTIVE
Moyamoya disease (MMD) is a rare entity that results in progressive occlusion of the arteries of the circle of Willis, but the pathogenesis of MMD is unknown.


METHODS
MMD sera (n=32) were tested for anti-endothelial cell antibodies by enzyme-linked immunoassays and flow cytometric analysis. Apoptosis was induced in human umbilical vein endothelial cells by tumor necrosis factor-alpha.


RESULTS
We found that a high proportion of MMD sera had anti-endothelial cell antibodies with apoptotic stimuli. Prominent reactivities of MMD sera (72%) with recombinant human alpha-fodrin were observed.


CONCLUSIONS
Our study demonstrates that MMD sera contain a high incidence of anti-alpha-fodrin autoantibodies, providing new insight into the mechanisms of occlusion of MMD arteries.
Ogawa
Kouichi
K
Department of Neurosurgery, University of Tokushima School of Medicine, Tokushima, Japan.
Nagahiro
Shinji
S
Arakaki
Rieko
R
Ishimaru
Naozumi
N
Kobayashi
Masaru
M
Hayashi
Yoshio
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000494:pharmacology; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D009072:Moyamoya Disease / Q000097:blood / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D013049:Spectrin
D001323:Autoantibodies; D002352:Carrier Proteins; D007074:Immunoglobulin G; D008840:Microfilament Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D013049:Spectrin