title: Effect of interstitial laser hyperthermia in a murine model of colorectal liver metastases: scanning electron microscopic study.
abstract: Interstitial laser hyperthermia (ILH) is an in situ ablative technique used to treat colorectal liver metastases. The relatively high recurrence of tumor after treatment by ILH may be related to incomplete destruction. Little is known about the effectiveness of ILH for destroying tumor microvasculature. The aim of this study was to define the changes to the microvascular architecture of tumors after treatment with ILH, specifically focusing on the completeness of tumor vasculature destruction. An intrasplenic induction model of liver metastases in 4- to 6-week-old male inbred CBA mice was used. Laser hyperthermia was applied to liver and tumor tissue using a bare optical quartz fiber from a Medilas Fibertom 4100 Nd:YAG surgical laser generator. The animals underwent microvascular corrosion casting of the livers immediately after application of ILH. Microvascular casts were then prepared and studied by scanning electron microscopy. ILH produced complete, uniform destruction of the tumor microvasculature with compete hemostasis. Blood flow in vessels larger than 100 microm diameter had a relatively protective effect, although ILH was able to overcome this barrier effectively by increasing the energy applied. ILH produces complete destruction of tumor microvasculature with hemostasis. The protective effect of blood flow in larger vessels can be overcome by the appropriate use of higher energy levels.
authors.affiliation: Department of Surgery, University of Melbourne, Austin Hospital, Studley Road, Heidelberg, 3084 Melbourne, Victoria, Australia.
authors.forename: Vijayaragavan
authors.initials: V
authors.lastname: Muralidharan
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Nikfarjam
authors.forename: Caterina
authors.initials: C
authors.lastname: Malcontenti-Wilson
authors.forename: Christopher
authors.initials: C
authors.lastname: Christophi
mesh_terms: D000818:Animals; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000473:pathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D006979:Hyperthermia, Induced / Q000379:methods; D053685:Laser Therapy; D008113:Liver Neoplasms / Q000556:secondary* / Q000628:therapy* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008855:Microscopy, Electron, Scanning