title: Validated HPLC method for determination of LASSBio-581, a new heterocyclic N-phenylpiperazine derivative, in rat plasma.
abstract: A rapid, simple and accurate high performance liquid chromatography (HPLC) method was developed and validated for the determination of LASSBio-581 (1-[1-(4-chloro-phenyl)-1H-[1,2,3]triazol-4-ylmethyl]-4-phenyl-piperazine) in rat plasma using ketoconazole as internal standard. Plasma samples were deproteinized with methanol. A good chromatographic separation was achieved using a reversed phase C18 column. Mobile phase consisting of sodium dihydrogen phosphate monohydrate (pH 4.5, 0.02 M) and methanol mixture (35:65, v/v) was used at a flow rate of 1.0 ml/min. The eluate was monitored using a UV detector at 248 nm. The retention times of LASSBio-581 and the internal standard were approximately 3.8 and 5.6 min, respectively. The calibration curves were linear over the concentration range of 0.25-8.0 microg/ml with correlation coefficients >0.99. The limit of quantitation was 0.25 microg/ml. The accuracy of the method was >90%. The intra-day relative standard deviation (R.S.D.) ranged from 6.15 to 10.52% at 0.4 microg/ml, 7.44 to 13.81% at 1.5 microg/ml and 6.10 to 13.94% at 6.0 microg/ml. The inter-day R.S.D. were 9.54, 8.42 and 8.25% at 0.4, 1.5 and 6.0 microg/ml, respectively. No interference from endogenous substances or metabolites were observed. The method has been used to measure plasma concentrations of LASSBio-581 in pharmacokinetic studies in rats.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Tasso
authors.forename: G
authors.initials: G
authors.lastname: Neves
authors.forename: R
authors.initials: R
authors.lastname: Menegatti
authors.forename: C A M
authors.initials: CA
authors.lastname: Fraga
authors.forename: E J
authors.initials: EJ
authors.lastname: Barreiro
authors.forename: V L
authors.initials: VL
authors.lastname: Eifler-Lima
authors.forename: S M K
authors.initials: SM
authors.lastname: Rates
authors.forename: Teresa
authors.initials: T
authors.lastname: Dalla Costa
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006571:Heterocyclic Compounds / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D010879:Piperazines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006571:Heterocyclic Compounds; C490224:LASSBio-581; D010879:Piperazines; C031503:phenylpiperazine