title: Simultaneous transgenic suppression of LePG and LeExp1 influences rheological properties of juice and concentrates from a processing tomato variety.
abstract: Processing tomato lines suppressed in the accumulation of ripening-related polygalacturonase or expansin were generated by introduction of transgenes to silence expression of the LePG and LeExp1 genes, respectively. The rheological properties of juice and juice reconstituted from paste produced from lines suppressed in one of these genes, or in both, were compared with azygous controls. When assayed by measuring Bostwick consistency, paste produced from either suppressed LePG or suppressed LeExp1 lines and diluted to 5 degrees Brix was approximately 18% more viscous than that produced from controls. Simultaneous suppression of LePG and LeExp1 produced a small additional increase in viscosity of 4%. Rheometric flow analysis at 5 or 10 degrees Brix also showed substantial increases in the consistency index due to suppression of either LePG or LeExp1 alone, and a small additional increase when both genes were suppressed in the same transgenic line. Measurements by laser diffraction and [1H]NMR showed that suppression of LePG or LeExp1 accumulation altered the size distribution of insoluble particles and modified their surface properties. The data are consistent with suppression of LePG increasing serum viscosity, and suppression of either LePG or LeExp1 altering the properties of the insoluble particles and improving some aspect of particle-particle or particle-serum interaction, or both. However, relative to that caused by suppression of either gene alone, the additional increase in viscosity caused by simultaneous suppression of LePG and LeExp1 together was slight.
authors.affiliation: Department of Food Science and Technology, University of California, Davis, California 95616.
authors.forename: Mary S
authors.initials: MS
authors.lastname: Kalamaki
authors.forename: Mark H
authors.initials: MH
authors.lastname: Harpster
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Palys
authors.forename: John M
authors.initials: JM
authors.lastname: Labavitch
authors.forename: David S
authors.initials: DS
authors.lastname: Reid
authors.forename: David A
authors.initials: DA
authors.lastname: Brummell
mesh_terms: D001628:Beverages; D005511:Food Handling; D005524:Food Technology; D018551:Solanum lycopersicum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D010316:Particle Size; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology*; D011096:Polygalacturonase / Q000235:genetics*; D012212:Rheology; D013489:Suppression, Genetic
chemical_list: D010940:Plant Proteins; C095825:expansin protein, plant; D011096:Polygalacturonase