Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections.
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to identify an isolate of S. haemolyticus fully. The PCR assay, which detects polymorphism in the 16S-23S rRNA spacer region, is shown to be potentially useful for rapid and accurate identification of coagulase-negative staphylococci. Identical PFGE type and antibiotic-resistance profiles of two methicillin-resistant S. haemolyticus isolates in this study suggest the existence of a multiresistant community clone.
Shittu
Adebayo
A
School of Biochemistry and Microbiology, University of Durban-Westville, Durban 4000, Republic of South Africa 2Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, UK 3Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
Lin
Johnson
J
School of Biochemistry and Microbiology, University of Durban-Westville, Durban 4000, Republic of South Africa 2Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, UK 3Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
Morrison
Donald
D
School of Biochemistry and Microbiology, University of Durban-Westville, Durban 4000, Republic of South Africa 2Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, UK 3Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
Kolawole
Deboye
D
School of Biochemistry and Microbiology, University of Durban-Westville, Durban 4000, Republic of South Africa 2Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, UK 3Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
D000328:Adult; D002648:Child; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D018461:Soft Tissue Infections / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013207:Staphylococcal Skin Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D041162:Staphylococcus haemolyticus / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D014946:Wound Infection / Q000382:microbiology*
D004269:DNA, Bacterial