Case study of the distribution of mucosa-associated Bifidobacterium species, Lactobacillus species, and other lactic acid bacteria in the human colon.
The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, Lactobacillus group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with Lactobacillus group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site.
Nielsen
D S
DS
Department of Dairy and Food Science, Center for Advanced Food Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Møller
P L
PL
Rosenfeldt
V
V
Paerregaard
A
A
Michaelsen
K F
KF
Jakobsen
M
M
D000328:Adult; D001644:Bifidobacterium / Q000302:isolation & purification*; D003106:Colon / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D007777:Lactobacillaceae / Q000302:isolation & purification*; D007778:Lactobacillus / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S