Interleukin-1 and nitric oxide increase NADPH oxidase activity in human coronary artery smooth muscle cells.
OBJECTIVE
Cytokines, nitric oxide (NO) and reactive oxygen species (ROS) are well known for their pathogenic effects in development of cardiovascular diseases. Interleukin-1beta (IL-1beta) is known to induce NO generation, however it is not well established if IL-1beta or NO regulate production of ROS, such as superoxide anion. Therefore, the main objective of this study was to evaluate the effect of IL-1beta or NO on enzyme activity of NADPH oxidase (NOX), a superoxide-generating system recently documented to participate in a variety of vascular functions.


METHODS
Human coronary artery smooth muscle cells (SMC) obtained from Clonetics were treated with IL-1beta and NO donor, sodium nitroprusside (SNP), in culture. Nitrites accumulated in supernatants of SMC cultures were measured as an index of NO released following treatment with IL-1beta. NOX enzyme activity was assayed using cytochrome c as the electron acceptor.


RESULTS
Treatment with IL-1beta resulted in a 3-fold increase in the production of NO by SMC. Both IL-1beta and SNP enhanced NOX activity, by 67 and 45%, respectively, following 24 h of treatment.


CONCLUSIONS
This study suggests that NO or NO- generating cytokines might regulate the production of ROS in the cardiovascular system through modulation of superoxide-generating systems such as NOX.
Kaur
Jaspal
J
Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA.
Dhaunsi
Gursev S
GS
Turner
Ronald B
RB
D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D007375:Interleukin-1; D020030:Nitric Oxide Donors; D013481:Superoxides; D009599:Nitroprusside; D009569:Nitric Oxide; D019255:NADPH Oxidases