Extraction and analysis of human nuclear and mitochondrial DNA from electron beam irradiated envelopes.
The United States Postal Service is considering methods such as electron beam irradiation to neutralize biological agents sent through the mail. While this is proven to reduce/eliminate pathogenic organisms, it may also degrade human genomic DNA and therefore hinder the ability to garner forensically informative genetic profiles. To determine the effects of electron beam irradiation on DNA typing, 16 white, standard letter-sized envelopes were licked. Half of the envelopes served as nonirradiated controls while the other half underwent irradiation at dosages sufficient to kill anthrax spores (29.3 and 51.6 kGy). Total cellular DNA was extracted from all envelopes; nuclear short tandem repeat loci, as well as the hypervariable region I from mitochondrial DNA, were amplified by means of the polymerase chain reaction. Short tandem repeat profiles and mitochondrial DNA sequence haplotypes were acquired on an ABI Prism 310 Genetic Analyzer platform. Analysis of data from irradiated samples revealed evidence of DNA degradation; however, the ability to construct full genetic profiles from both nuclear and mitochondrial DNA remained largely unaffected. The use of the polymerase chain reaction, coupled with florescent fragment analysis and mitochondrial DNA sequencing, should be considered to profile biological material from evidence enduring irradiation to inactivate infectious agents.
Withrow
Angela G
AG
Marshall University Forensic Science Center, DNA Laboratory, Huntington, WV 25701, USA.
Sikorsky
Jan
J
Downs
J C Upshaw
JC
Fenger
Terry
T
D022801:Complementarity Determining Regions / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000528:radiation effects*; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D005554:Forensic Medicine / Q000379:methods*; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction; D011177:Postal Service; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D014481:United States
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D004247:DNA