Application of polyaniline/sol-gel derived tetraethylorthosilicate films to an amperometric lactate biosensor.
The electrochemical entrapment of polyaniline (PANI) onto sol-gel derived tetraethylorthosilicate (TEOS) films deposited onto indium-tin-oxide (ITO) coated glass has been utilized for immobilization of lactate dehydrogenase (LDH). The performance of these sol-gel/PANI/LDH electrodes has been investigated as a function of the lactate concentration, applied potential, pH of the medium and interferents. The amperometric response of the electrodes under optimum conditions exhibited a linear relationship from 1 mM to 4 mM. An attempt has been made to extend the linearity up to 10 mM for lactate by coating an external layer of polyvinyl chloride (PVC) over the sol-gel/PANI/LDH electrodes with a correlation coefficient of 0.89. These sol-gel/PANI/LDH electrodes have a response time of about 60 s, a shelf life of about 8 weeks at 0-4 degrees C and have implications in a lactate biosensor.
Chaubey
Asha
A
Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi-110012, India.
Pande
K K
KK
Malhotra
B D
BD
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis*; D009243:NAD / Q000737:chemistry; D044367:Phase Transition; D011143:Polyvinyl Chloride / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*
D000814:Aniline Compounds; D012821:Silanes; C416807:polyaniline; D009243:NAD; D019344:Lactic Acid; C040733:tetraethoxysilane; D011143:Polyvinyl Chloride; D007770:L-Lactate Dehydrogenase