[Cloning and sequence of human hepatocyte DNA synthetic stimulated factor cDNA].
OBJECTIVE
To clarify the nature of human hepatocyte DNA synthetic stimulated factor (HDSSF) and to lay a foundation for its gene cloning and expression.


METHODS
We amplified the target gene fragment by degenerating PCR, labelled the fragment as a probe to screen human fetal liver cDNA library, and then analyzed the HDSSF gene sequence.


RESULTS
A 600 base pair fragment was obtained and labelled successfully with DIG. Human HDSSF sequence was determined by screening the library and gene sequencing. Its cDNA chain was composed of 748 base pairs including 594 base pair open read-code frame and 5' and 3' terminal noncode district sequence.


CONCLUSIONS
By screening cDNA library, the HDSSF gene cloning has been gained successfully. HDSSF should be a new hepatocyte growth substance which consists of 198 amino acid residues.
Tang
Shi-gang
SG
Department of Infectious Diseases, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Su
Xian-shi
XS
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; C485002:hepatocyte DNA synthetic stimulated factor, human; D017228:Hepatocyte Growth Factor