False identification of Coccidioides immitis: do molecular methods always get it right?
rRNA sequence analysis of a partial region of the 18S and 5.8S-internal transcribed spacer 2 (ITS2) region of Chrysosporium keratinophilum highlights its potential molecular misidentification as Coccidioides immitis. Molecular identification of medically important fungi should not be based solely on sequence analysis of the 18S rRNA gene but should be confirmed by sequence analysis of an additional rRNA gene locus, such as the ITS region(s).
Millar
B Cherie
BC
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom.
Jiru
Xu
X
Walker
Michael J
MJ
Evans
James P
JP
Moore
John E
JE
D001483:Base Sequence; D002912:Chrysosporium / Q000145:classification* / Q000235:genetics*; D003045:Coccidioides / Q000145:classification* / Q000235:genetics*; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015203:Reproducibility of Results; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D021901:DNA, Intergenic; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S