title: A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum-infected erythrocytes.
abstract: Severe malaria by Plasmodium falciparum is a potentially fatal disease, frequently unresponsive to even the most aggressive treatments. Host organ failure is associated with acquired rigidity of infected red blood cells and capillary blockage. In vitro techniques have played an important role in modeling cell deformability. Although, historically they have either been applied to bulk cell populations or to measure single physical parameters of individual cells. In this article, we demonstrate the unique abilities and benefits of elastomeric microchannels to characterize complex behaviors of single cells, under flow, in multicellular capillary blockages. Channels of 8-, 6-, 4-, and 2-microm widths were readily traversed by the 8 microm-wide, highly elastic, uninfected red blood cells, as well as by infected cells in the early ring stages. Trophozoite stages failed to freely traverse 2- to 4-microm channels; some that passed through the 4-microm channels emerged from constricted space with deformations whose shape-recovery could be observed in real time. In 2-microm channels, trophozoites mimicked "pitting," a normal process in the body where spleen beds remove parasites without destroying the red cell. Schizont forms failed to traverse even 6-microm channels and rapidly formed a capillary blockage. Interestingly, individual uninfected red blood cells readily squeezed through the blockages formed by immobile schizonts in a 6-microm capillary. The last observation can explain the high parasitemia in a growing capillary blockage and the well known benefits of early blood transfusion in severe malaria.
authors.affiliation: Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.
authors.forename: J Patrick
authors.initials: JP
authors.lastname: Shelby
authors.forename: John
authors.initials: J
authors.lastname: White
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Ganesan
authors.forename: Pradipsinh K
authors.initials: PK
authors.lastname: Rathod
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D000818:Animals; D002196:Capillaries / Q000469:parasitology*; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology*; D006461:Hemolysis; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008962:Models, Theoretical; D010963:Plasmodium falciparum / Q000472:pathogenicity*; D013997:Time Factors