Evaluation of Carolina Rinse solution as a treatment for ischaemia reperfusion of the equine jejunum.
BACKGROUND
Ileus and peritoneal adhesions are the most common complications following surgery for small intestinal obstruction. Carolina Rinse (CR) has been shown to decrease reperfusion injury in intestine and other organs.


OBJECTIVE
CR decreases intestinal inflammation and subsequent scarring associated with reperfusion injury.


METHODS
CR was infused intra-arterially and applied topically just prior to reperfusion in jejunum exposed to experimental ischemia. Vascular permeability, neutrophil accumulation and serosal scarring were compared in treated and untreated intestine.


RESULTS
CR maintained a normal osmotic reflection coefficient and decreased migration of neutrophils into the serosa during reperfusion. After 10 days, treated intestine was normal in appearance with a trend toward less serosal scarring and fibroblast proliferation. There was a significant decrease in fibroplasia at biopsy sites in treated intestine.


CONCLUSIONS
Arterial perfusion combined with topical application of CR during jejunal ischaemia decreases immediate reperfusion injury and limits post operative scarring.


CONCLUSIONS
CR should be used as a local perfusate rather than a systemic treatment; it may best be applied topically and intraluminally to avoid damaging mesenteric arteries. CR should be considered an adjunct treatment as part of overall surgical management and post operative care.
Dabareiner
R M
RM
The Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, PO Box 1938, Leesburg, Virginia 20177, USA.
White
N A
NA
Donaldson
L
L
D000287:Administration, Topical; D000818:Animals; D002921:Cicatrix / Q000517:prevention & control / Q000662:veterinary; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D005260:Female; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D007261:Infusions, Intra-Arterial / Q000662:veterinary; D007583:Jejunum / Q000098:blood supply / Q000187:drug effects / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D012039:Regional Blood Flow / Q000187:drug effects; D015427:Reperfusion Injury / Q000188:drug therapy / Q000662:veterinary*; D012996:Solutions / Q000008:administration & dosage / Q000627:therapeutic use*; D013152:Splanchnic Circulation / Q000187:drug effects; D016896:Treatment Outcome
C070887:Carolina rinse solution; D012996:Solutions