Oxidative stress in HEp-2 human laryngeal carcinoma cells induced by combination of vitamins B12b and C.
Incubation of human laryngeal epidermoid carcinoma HEp-2 cells with hydroxocobalamin (vitamin B12b) and ascorbic acid (vitamin C) for 1 h initiated oxidative stress accompanied by damage to mitochondria and increase in intracellular oxidative activity. Studies of the kinetics of these processes showed that the increase in intracellular H2O2 activity and mitochondrial damage are more likely a result, but not the cause of cell apoptosis during the first hour of their incubation with vitamins B12b and C.
Akatov
V S
VS
Tissue Engineering Group, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino. v-akatoff@mail.ru
Solov'eva
M E
ME
Leshchenko
V V
VV
Teplova
V V
VV
D001205:Ascorbic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D016923:Cell Death; D045744:Cell Line, Tumor; D005452:Fluoresceins / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006879:Hydroxocobalamin / Q000494:pharmacology*; D007700:Kinetics; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008564:Membrane Potentials; D008928:Mitochondria / Q000473:pathology; D018384:Oxidative Stress; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014805:Vitamin B 12 / Q000494:pharmacology*
D005452:Fluoresceins; D005456:Fluorescent Dyes; C029569:diacetyldichlorofluorescein; D006861:Hydrogen Peroxide; D014805:Vitamin B 12; D001205:Ascorbic Acid; D006879:Hydroxocobalamin; D002118:Calcium