[Analysis on the SARS-CoV genome of PUMC01 isolate].
OBJECTIVE
To perform variation and phylogenetics analysis on the SARS-CoV genome sequence (PUMC01) isolated in the Peking Union Medical College Hospital.


METHODS
The cDNA library of SARS-CoV (PUMC01 isolate) was constructed by means of random-priming strategy. Random selected plasmid was sequenced and the genome sequence of SARS-CoV-PUMC01 was assembled by conventional methods (The Genebank Accession No. of SARS-CoV-PUMC01 is AY350750). The variation and phylogenetics analysis were performed by comparing the PUMC01 sequence with other SARS-CoV isolates.


RESULTS
Ten variation sites were found by comparing PUMC01 isolate with Tor2 and Urbani isolates. In phylogenetic analysis of 18 SARS-CoV isolates, two classes were observed and there is different differential time between these two classes and the different isolates in each class.


CONCLUSIONS
The evidence of phylogenetic analysis of different SARS-CoV isolates from different region is instructive for understanding the clinical relations between the different isolates and the transmission chain of SARS-CoV.
Zou
Ke
K
Chinese Academy of Medical Sciences-Chinese National Human Genome Center, Beijing SARS Coronavirus Genome Sequence Research Group, Beijing 100005, China.
Zhu
Hua
H
Ding
Ke-yue
KY
Wang
Zhong
Z
Liu
Yong
Y
Wang
Ting
T
Yang
Jian
J
Wei
Guo-zhu
GZ
Zhou
Xin-feng
XF
Zhang
Wen
W
Yu
Zhan-xia
ZX
Fan
Zheng
Z
Peng
Xiao-zhong
XZ
Qin
Chuan
C
Liu
Xiang-jun
XJ
Shen
Yan
Y
Ni
An-ping
AP
Qiang
Bo-qin
BQ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins