Evaluation of the Abbott Cell-Dyn 4000 hematology analyzer for detection and therapeutic monitoring of Plasmodium vivax in the Republic of Korea.
The Cell-Dyn 4000 automated hematology analyzer (CD4000) has the ability to detect malaria patients, but it remained unclear whether it could detect persistent malaria post-treatment. To investigate this, we used the CD4000 to evaluate 68 Korean patients with Plasmodium vivax malaria, and control groups of 50 patients with fever and 50 asymptomatic patients. The results from the instrument-generated scatter plot (derived by laser light depolarization) were compared with microscopy results. During the initial diagnosis, the sensitivity of the CD4000 in detecting malaria was 91.2%. On day 3 of follow-up, the CD4000 results matched those from microscopy by 96.7%. Malaria was not detected by either method beyond 14 days post-presentation. Interestingly, the atypical depolarizing events, which typify the presence of malaria in the analyzer, were highly correlated with the levels of parasitaemia in serially diluted samples of the leucocyte-depleted blood, and the CD4000 detected parasites down to the level of 288 +/- 17.7/microl. Our findings suggest that the phenomenon of atypical light depolarization could be influenced by parasitaemia levels, and be used as a screening method for P. vivax malaria patients, as well as for the therapeutic monitoring.
Suh
In Bum
IB
Department of Laboratory Medicine, Travel Medicine Clinic, College of Medicine, Korea University, South Korea. Chicago@hanmir.com
Kim
Hae Joong
HJ
Kim
Jin Yong
JY
Lee
Sang Woo
SW
An
Seong Soo A
SS
Kim
Woo Ju
WJ
Lim
Chae Seung
CS
D000328:Adult; D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000188:drug therapy; D008875:Middle Aged; D010274:Parasitology / Q000295:instrumentation / Q000379:methods; D010966:Plasmodium vivax / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000962:Antimalarials