[Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips].
A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-line drugs, i.e. rifampin and isoniazid. The method provides for detecting above 95% of rifampin-resistant and around 80% of isoniazid-resistant strains within 1 day.
Griadunov
D A
DA
Mikhaĭlovich
V M
VM
Lapa
S A
SA
Rudinskiĭ
N I
NI
Barskiĭ
V E
VE
Chudinov
A V
AV
Zasedatelev
A S
AS
Mirzabekov
A D
AD
D001426:Bacterial Proteins; D002374:Catalase; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology
D001426:Bacterial Proteins; D009841:Oligonucleotides; D010088:Oxidoreductases; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid; D012293:Rifampin