Coding potential of laboratory and clinical strains of human cytomegalovirus.
Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.
Murphy
Eain
E
Department of Molecular Biology, Princeton University, Princeton, NJ 80544, USA.
Yu
Dong
D
Grimwood
Jane
J
Schmutz
Jeremy
J
Dickson
Mark
M
Jarvis
Michael A
MA
Hahn
Gabriele
G
Nelson
Jay A
JA
Myers
Richard M
RM
Shenk
Thomas E
TE
D022202:Chromosomes, Artificial, Bacterial; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D004279:DNA, Viral; D005347:Fibroblasts / Q000378:metabolism; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D004279:DNA, Viral; D004247:DNA