title: Evaluation of the analytic performances of the new HPLC system HLC-723 G7 for the measurement of hemoglobin A1c.
abstract: OBJECTIVE
  The analytical performance of a new automated HPLC system, for the determination of HbA1C in blood (Tosoh HLC-723 G7), was studied.
  
  
  METHODS
  The study design included the evaluation of imprecision, linearity, interference and carryover. Comparison study was performed by comparing HbA1C results with those obtained from an established method (Bio-Rad Variant II).
  
  
  RESULTS
  Total imprecision was less than 1.34% and the results were linear up to 17.2% HbA1C. The method showed a wide analytical range, and no carryover between specimens. Comparison study yielded, r=0.989, Sy.x=0.255, regression equation (y=0.9895x-0.35); Bland-Altman plot showed a mean bias=- 0.43% HbA1C with confidence limits ranging from -0.48% to -0.38% HbA1C. The presence of abnormal hemoglobin was clearly revealed, and no interference from labile HbA1C was apparent.
  
  
  CONCLUSIONS
  The HLC-723 G7 instrument seems to be a reliable system for routine assay of HbA1C.
authors.affiliation: Central Clinical Biochemistry Laboratory, Hospital of Careggi, viale Morgagni 85, 50139 Florence, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Terreni
authors.forename: Renata
authors.initials: R
authors.lastname: Paleari
authors.forename: Anna
authors.initials: A
authors.lastname: Caldini
authors.forename: Agostino
authors.initials: A
authors.lastname: Ognibene
authors.forename: Andrea
authors.initials: A
authors.lastname: Mosca
authors.forename: Gianni
authors.initials: G
authors.lastname: Messeri
mesh_terms: D001663:Bilirubin / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods* / Q000706:statistics & numerical data; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D014280:Triglycerides / Q000097:blood
chemical_list: D001663:Bilirubin; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; D014280:Triglycerides