title: Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatography-tandem mass spectrometry.
abstract: This paper presents the development of an analytical procedure for the determination of two sexual steroid hormones: 17beta-estradiol and estrone, and the synthetic contraceptive estrogen, 17alpha-ethynylestradiol in effluents of wastewater treatment plants. Samples are extracted via solid-phase extraction using C18 cartridges. Extracts in ethyl acetate are then purified with a liquid-liquid separation with aqueous sodium chloride, then with a clean-up on a Florisil cartridge. Steroids are analyzed using an LC-MS-MS ion trap system. Internal quantification with the corresponding deuterated steroids leads to limits of quantification at 5 ng/l for estrone and 10 ng/l for estradiol and ethynylestradiol. In mineral spiked water, recoveries are 91% for 17beta-estradiol, 97% for estrone and 87% for 17alpha-ethynylestradiol and RSDs are 15% for 17beta-estradiol, 11% for estrone and 23% for 17alpha-ethynylestradiol.
authors.affiliation: Anjou Recherche-Vivendi Water, Emerging Parameters, 1 Place de Turenne, Immeuble Le Dufy, 94417 Saint Maurice Cedex, France. valerie.ingrand@generale-des-eaux.net
authors.forename: Valerie
authors.initials: V
authors.lastname: Ingrand
authors.forename: Gaëla
authors.initials: G
authors.lastname: Herry
authors.forename: Johanne
authors.initials: J
authors.lastname: Beausse
authors.forename: Marie-Renée
authors.initials: MR
authors.lastname: de Roubin
mesh_terms: D004967:Estrogens / Q000032:analysis*; D007220:Industrial Waste; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004967:Estrogens; D007220:Industrial Waste; D014874:Water Pollutants, Chemical