Putative FLJ20436 gene characterisation in goat. Observed ubiquitous expression in goat and transgenic mice allowed to restrict the location of an hypothesised insulator element.
Eukaryotic genomes are organised into independent domains through the establishment of boundaries which allow to have distinct pattern of gene expression both during development and in differentiated cells. The previously reported site independent expression of the mammary-specific goat alpha-lactalbumin gene in transgenic mice suggested the existence of cis-regulatory elements located upstream of this gene. The nearby presence of a second ubiquitously expressed gene (the cyclin T1 gene) allowed to define two chromatin domains putatively separated by a boundary insulator-like element. In this study, the characterisation of a third putative gene (FLJ20436) present between the alpha-lactalbumin and the cyclin T1 loci is reported. It was found to be a functional gene, ubiquitously expressed both in goat and transgenic mice. A complex pattern of alternative splicing events was observed in several analysed tissues leading to various mRNAs and putative FLJ20436 proteins, as suggested in human and mouse species. This allowed us to assign this gene to one of the two hypothesised chromatin domains, refining the location of the potential insulator element.
Mata
Xavier
X
Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas Cedex, France. mata@diamant.jouy.inra.fr
Taourit
Sead
S
Le Provost
Fabienne
F
D017398:Alternative Splicing; D000818:Animals; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D011061:Poly A