Increase in hepatic mRNA expression of coagulant factors in type 2 diabetic model mice.
OBJECTIVE
The purpose of the study is to investigate whether hypercoagulation in diabetes is observed not only in increased plasma levels of the coagulative factors but also in increased hepatic mRNA levels.


METHODS
The age-related changes of coagulation factors were determined using KK and KK-A(y) mice as a model for human type 2 diabetes mellitus. Expression of the alpha-, beta- and gamma-chains of fibrinogen-mRNA and prothrombin-mRNA from the mouse liver was examined by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Hemoglobin A(1c) (HbA(1C)), plasma fibrinogen, triglyceride and insulin levels increased apparently, especially in KK-A(y) mouse at 4 months old. The mRNA levels of gamma-chain of fibrinogen significantly increased at 3 and 4 months of age in both mice. The mRNA levels of alpha- and beta-chains of fibrinogen and prothrombin were significantly increased in 4-month-old KK-A(y) mouse.


CONCLUSIONS
These results suggest that the increase in hepatic mRNA expression of coagulant factors contributes to the hypercoagulable state in type 2 diabetic mice.
Murakami
Hidetomo
H
Department of Pharmacology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. hideneurom@med.showa-u.ac.jp
Okazaki
Masako
M
Amagasa
Hidetoshi
H
Oguchi
Katsuji
K
D000818:Animals; D001779:Blood Coagulation Factors / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004195:Disease Models, Animal; D005340:Fibrinogen / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D011516:Prothrombin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics
D001779:Blood Coagulation Factors; D018076:DNA, Complementary; D012333:RNA, Messenger; D011516:Prothrombin; D005340:Fibrinogen