Site-specific recombination by the DDE family member mobile element IS30 transposase.
DNA rearrangements carried out by site-specific recombinases and transposases (Tpases) show striking similarities despite the wide spectrum of the catalytic mechanisms involved in the reactions. Here, we show that the bacterial insertion sequence (IS)30 element can act similarly to site-specific systems. We have developed an inversion system using IS30 Tpase and a viable lambda phage, where the integration/excision system is replaced with IS30. Both models have been proved to operate analogously to their natural counterpart, confirming that a DDE family Tpase is able to fulfill the functions of site-specific recombinases. This work demonstrates that distinction between transposition and site-specific recombination becomes blurred, because both functions can be fulfilled by the same enzyme, and both types of rearrangements can be achieved by the same catalytic mechanisms.
Kiss
János
J
Environmental Biosafety Research Institute, Agricultural Biotechnology Center, 4 Szent-Györgyi Albert Street, H-2100, Gödöllõ, Hungary.
Szabó
Mónika
M
Olasz
Ferenc
F
D020816:Amino Acid Motifs; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D017353:Gene Deletion; D008954:Models, Biological; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011995:Recombination, Genetic; D013997:Time Factors; D014158:Transcription, Genetic; D019895:Transposases / Q000235:genetics*
D004251:DNA Transposable Elements; D019895:Transposases