Photocrosslinkable polysaccharides based on chondroitin sulfate.
Photopolymerizing hydrogels have demonstrated potential for use as a scaffold in numerous tissue-engineering applications. The majority of photopolymerizing hydrogels are made from purely synthetic polymers. The purpose of this study was to synthesize and characterize photopolymerizing hydrogels derived from the biopolymer chondroitin sulfate in order to enhance the bioactivity of the scaffold and potentially improve tissue regeneration. Methacrylate groups were added to chondroitin sulfate, a major component of cartilage, using glycidyl methacrylate. The gels exhibited viscoelastic behavior typical of hydrogels. Cogels based on chondroitin sulfate and poly(ethylene glycol) demonstrated increasing pore size with increasing concentration of chondroitin sulfate as determined by water content, mechanical strength, and morphology using scanning electron microscopy. The chondroitin sulfate hydrogels degraded specifically in the presence of the enzyme chondroitinase. Chondrocytes remained viable after photoencapsulation and incubation in the biogels, suggesting their possible use for cartilage tissue engineering.
Li
Qiang
Q
Department of Biomedical Engineering, Johns Hopkins University, 3400 North Charles Street/Clark Hall 106, Baltimore, Maryland 21218, USA. jhe@bme.jhu.edu
Williams
Christopher G
CG
Sun
Danny D N
DD
Wang
Jun
J
Leong
Kam
K
Elisseeff
Jennifer H
JH
D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D002470:Cell Survival; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D003432:Cross-Linking Reagents; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D014132:Trachea / Q000737:chemistry / Q000166:cytology
D001672:Biocompatible Materials; D002214:Capsules; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011134:Polysaccharides; D002809:Chondroitin Sulfates