title: Xenopus Meis3 protein forms a hindbrain-inducing center by activating FGF/MAP kinase and PCP pathways.
abstract: Knockdown studies in Xenopus demonstrated that the XMeis3 gene is required for proper hindbrain formation. An explant assay was developed to distinguish between autonomous and inductive activities of XMeis3 protein. Animal cap explants caudalized by XMeis3 were recombined with explants neuralized by the BMP dominant-negative receptor protein. XMeis3-expressing cells induced convergent extension cell elongations in juxtaposed neuralized explants. Elongated explants expressed hindbrain and primary neuron markers, and anterior neural marker expression was extinguished. Cell elongation was dependent on FGF/MAP-kinase and Wnt-PCP activities. XMeis3 activates FGF/MAP-kinase signaling, which then modulates the PCP pathway. In this manner, XMeis3 protein establishes a hindbrain-inducing center that determines anteroposterior patterning in the brain.
authors.affiliation: Department of Biochemistry, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
authors.forename: Emil
authors.initials: E
authors.lastname: Aamar
authors.forename: Dale
authors.initials: D
authors.lastname: Frank
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000502:physiology; D009479:Neuropeptides / Q000502:physiology; D010060:Ovulation; D012249:Rhombencephalon / Q000196:embryology*; D014981:Xenopus / Q000196:embryology*; D029867:Xenopus Proteins / Q000235:genetics
chemical_list: D019485:Bone Morphogenetic Proteins; D018398:Homeodomain Proteins; C491174:Meis3 protein, Xenopus; D009479:Neuropeptides; D029867:Xenopus Proteins; D005346:Fibroblast Growth Factors; D020928:Mitogen-Activated Protein Kinases