title: YmoA negatively regulates expression of invasin from Yersinia enterocolitica.
abstract: inv encodes invasin, which is the primary invasion factor of Yersinia enterocolitica. inv expression in vitro is regulated in response to temperature, pH, and growth phase. In vitro, inv is maximally expressed at 26 degrees C and repressed at 37 degrees C at neutral pH but, when the pH of the media is adjusted to 5.5, levels of inv expression at 37 degrees C are comparable to those at 26 degrees C. A previous genetic screen for regulators of inv identified RovA, which was found to be required for activation of inv in vitro under all conditions tested as well as in vivo. Here we describe a screen that has identified a negative regulator of inv expression, ymoA. The ymoBA locus was identified by transposon mutagenesis as a repressor of inv expression in vitro at 37 degrees C at neutral pH. This mutant shows increased inv expression at 37 degrees C. The mutant can be fully complemented for inv expression by a plasmid expressing ymoA. These results indicate that YmoA plays a role in the negative regulation of inv.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: Damon W
authors.initials: DW
authors.lastname: Ellison
authors.forename: Briana
authors.initials: B
authors.lastname: Young
authors.forename: Kristin
authors.initials: K
authors.lastname: Nelson
authors.forename: Virginia L
authors.initials: VL
authors.lastname: Miller
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000469:Alkaline Phosphatase; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic / Q000502:physiology; D013696:Temperature; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C079210:YmoA protein, Yersinia enterocolitica; C053476:invasin, Yersinia; D018844:Cyclin-Dependent Kinases; D000469:Alkaline Phosphatase