[Determination of the stable mitochondrial factor in vivo].
We have determined a release of a stable mitochondrial factor (SMF) into the outflowing blood in vivo. That effect was induced by ischemia/reperfusion or phenylarsin oxide (PAO)--the activators of the mitochondrial permeability transition pore (MPTP) and the oxidative stress. The SMF was measured by a spectrophotometer in the range of wave-lengths 230-260 nm with the absorption maximum of 240-250 nm. The SMF release was accompanied with a decrease in the myocardial contractility, disturbances in cardiodynamics and regional blood circulation. The data obtained have demonstrated that the SMF can be used as a marker of MPTP opening in vivo.
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A.A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev.
Bohuslavs'kyĭ
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D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D003326:Coronary Circulation / Q000502:physiology; D004285:Dogs; D006614:Hindlimb / Q000098:blood supply; D007473:Ion Channels / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D024101:Mitochondrial Proteins / Q000097:blood*; D009200:Myocardial Contraction / Q000502:physiology; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D012039:Regional Blood Flow / Q000502:physiology
D001152:Arsenicals; D015415:Biomarkers; D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D024101:Mitochondrial Proteins; C029341:oxophenylarsine