A quantitative analysis of the expression of alpha-smooth muscle actin in mesangioproliferative (GnMes) glomerulonephritis.
The aim of the study was to quantitatively assess the expression of alpha-SMA in the renal interstitium in GnMes cases. Two image analysis approaches (in grey scale and in spatial colour images) were used to determine correctly the presence of the reaction. In spatial evaluation, the mean area fraction of a-SMA in the renal interstitium was 3.63% +/- 1.29%, while the results obtained from traditional quantitative method based on grey scale images were biased and too low.
Nieruchalska
Edyta
E
Laboratory of Morphometry and Medical Image Processing, Chair of Pathology, University of Medical Sciences,Poznań, Poland.
Strzelczyk
Rafał
R
Woźniak
Aldona
A
Zurawski
Jakub
J
Kaczmarek
Elzbieta
E
Salwa-Zurawska
Wiesława
W
D000199:Actins / Q000032:analysis / Q000378:metabolism*; D005355:Fibrosis / Q000378:metabolism / Q000473:pathology; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology
D000199:Actins