Novel methyltransferase for modified uridine residues at the wobble position of tRNA.
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.
Kalhor
Hamid R
HR
Department of Chemistry and Biochemistry, UCLA Molecular Biology Institute, University of California-Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.
Clarke
Steven
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D008745:Methylation; D008969:Molecular Sequence Data; D009154:Mutation; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D012347:RNA, Transfer, Arg / Q000737:chemistry / Q000378:metabolism; D012352:RNA, Transfer, Glu / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature; D014529:Uridine / Q000737:chemistry; D012359:tRNA Methyltransferases / Q000235:genetics / Q000378:metabolism*
D004271:DNA, Fungal; D012331:RNA, Fungal; D012347:RNA, Transfer, Arg; D012352:RNA, Transfer, Glu; D029701:Saccharomyces cerevisiae Proteins; D012343:RNA, Transfer; C480488:TRM9 protein, S cerevisiae; D012359:tRNA Methyltransferases; D014529:Uridine