[Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells].
OBJECTIVE
To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.


METHODS
With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank.


RESULTS
593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.


CONCLUSIONS
Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.
Wang
Dong-mei
DM
Department of Stem Cell Biology, Institute of Transfusion Medicine, Beijing 100850, China.
Yang
Li
L
Li
Liang
L
Feng
Kai
K
Bai
Ci-xian
CX
Chen
Lin
L
Pei
Xue-tao
XT
D018952:Antigens, CD34 / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization
D018952:Antigens, CD34