Anti-inflammatory effect of methoxyphenamine compound in rat model of chronic obstructive pulmonary disease.
OBJECTIVE
To evaluate the anti-inflammatory effect of methoxyphenamine compound (MC) on chronic obstructive pulmonary disease (COPD) in rats by measurement of proinflammatory cytokines, total and differential white cell counts (WCC) of bronchroalveolar lavage fluid (BALF).


METHODS
Adult rat model of COPD (COPD group) was induced by intratracheal instillation of lipopolysaccharides and exposure to cigarette smoke. Treatment groups received different dosage of MC (3, 9, and 27 mg daily, MC group) or prednisone (0.25 mg daily, P group) respectively. Tumor necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta), interleukin-6 (IL-6), transforming growth factor beta (TGF-beta) of BALF were determined by ELISA. Total and differential WCC were performed after Giemsa staining.


RESULTS
The levels of TNF-alpha, IL-1beta, IL-6, TGF-beta, total and differential WCC in BALF of MC groups were significantly decreased than that of COPD group (P<0.01), and there was no significant difference among MC groups. There was no significant decrease in the levels of TNF-alpha, IL-1beta and count of alveolar macrophages in P group compared to those of COPD group. More significant decrease in total WCC and neutrophils was found in P than in COPD group (P<0.01).


CONCLUSIONS
MC has anti-inflammmatory effect in the rats with COPD.
Wang
Yue-Hong
YH
Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Bai
Chun-Xue
CX
Hong
Qun-Ying
QY
Chen
Jie
J
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D009504:Neutrophils / Q000276:immunology; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D008694:Methamphetamine; C005157:methoxyphenamine