title: [Influence of different temperatures on internalization of epidermal growth factor receptor complexes in Tca-8113 cells].
abstract: OBJECTIVE
  To investigate the effect of different temperatures on internalization of epidermal growth factor receptor complexes in human tongue squamous carcinoma cells(Tca-8113).
  
  
  METHODS
  Human epidermal growth factor r (hEGF) was labeled with 125I according to Chloramine-T method. The quantity of surface binding and internalization of 125I-hEGF in Tca-8113 cells at 4 degrees C and at 37 degrees C was compared respectively with radiobinding assay.
  
  
  RESULTS
  At 37 degrees C, the amount of EGFR complexes internalization exceeded that of surface binding after 10 min. At 4 degrees C, the level of interlization maintained very low.
  
  
  CONCLUSIONS
  The study indicated that the surface binding of 125I-hEGF at 4 degrees C was steadier than that at 37 degrees C. The study on phenomenon of EGFR complexes internalization provide the experimental basis for selecting anti-EGFR monoclonal antibody in clinic.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University, Xi'an 710032, China. moyisun@fmmu.edu.cn
authors.forename: Mo-yi
authors.initials: MY
authors.lastname: Sun
authors.forename: Bao-lin
authors.initials: BL
authors.lastname: Liu
authors.forename: Yue-gui
authors.initials: YG
authors.lastname: Jing
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Shang
authors.forename: Jian-hu
authors.initials: JH
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D013696:Temperature
chemical_list: D007457:Iodine Radioisotopes; D004815:Epidermal Growth Factor; D066246:ErbB Receptors