title: Development of a bioanalytical liquid chromatography method for quantitation of 9-nitrocamptothecin in human plasma.
abstract: 9-Nitrocamptothecin (9-NC) is an orally administered camptothecin (CPT) that is under evaluation in clinical trials. This compound is not fluorescent, which has hampered development of a sensitive high-performance liquid chromatographic (LC) assay for measurement of drug concentrations in clinical trials. We now report development of an assay that involves reduction of 9-NC to the fluorescent compound 9-aminocamptothecin (9-AC). The method is based on enzymatic reduction of 9-NC using bovine liver S-9 fraction. This method is validated to quantitate 9-NC and 9-AC in patient samples, and yields results comparable to those obtained with an LC/MS method.
authors.affiliation: Department of Pharmacology, Robert Wood Johnson School of Medicine, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USA. goudemk@umdnj.edu
authors.forename: Murugesan K
authors.initials: MK
authors.lastname: Gounder
authors.forename: Show-Li
authors.initials: SL
authors.lastname: Sun
authors.forename: Howard
authors.initials: H
authors.lastname: Sands
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Weichung J
authors.initials: WJ
authors.lastname: Shih
authors.forename: Zheming
authors.initials: Z
authors.lastname: Gu
authors.forename: Samantha
authors.initials: S
authors.lastname: Charles-Williams
authors.forename: Moulakshi
authors.initials: M
authors.lastname: Roychowdhury
authors.forename: Rajeev
authors.initials: R
authors.lastname: Rajendra
authors.forename: Eric H
authors.initials: EH
authors.lastname: Rubin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D001711:Biotransformation; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood*; D002417:Cattle; D006801:Humans; D008099:Liver / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; C079905:rubitecan; D002166:Camptothecin