Characterization of tetrol I-1 and (+/-)-anti-BPDE-DNA adducts with solid-matrix fluorescence quenching.
The solid-matrix fluorescence (SMF) quenching of (+/-)-anti-benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts and a hydrolysis product of the DNA adducts, tetrol I-1, were investigated by using thallium nitrate and sodium iodide to quench the SMF. Several fluorescence quenching models were evaluated for both (+/-)-anti-BPDE-DNA adducts and tetrol I-1. The SMF quenching phenomena were quite different with the two salts for the (+/-)-anti-BPDE-DNA adducts and tetrol I-1. Generally, with thallium nitrate as a quencher, a two-site model with two independent quenching sites was applicable to both the (+/-)-anti-BPDE-DNA adducts and tetrol I-1 data. However, with sodium iodide, the SMF quenching data for tetrol I-1 were fit to the sphere of action model, but the (+/-)-anti-BPDE-DNA adducts SMF quenching data were qualitatively related to a BET isotherm. From the SMF quenching data, unique information was acquired for the quasi-intercalated BPDE-DNA adducts and the external form of the BPDE-DNA adducts. In addition, insights were obtained on how the adsorbed salts interacted with the solid matrix and with the (+/-)-anti-BPDE-DNA adducts and tetrol I-1.
Smith
Barry W
BW
Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071-3838, USA.
Hurtubise
Robert J
RJ
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000737:chemistry*; D001564:Benzo(a)pyrene / Q000031:analogs & derivatives* / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D018736:DNA Adducts; D001564:Benzo(a)pyrene; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C074998:benzo(a)pyrene-7,8,9,10-tetrol