title: Preparation of deuterated methyl and dimethyl substituted nicotinoylating agents for derivatization of the N-terminal of protein.
abstract: Methyl groups of 6-methylnicotinic acid and 2,6-dimethylnicotinic acid were deuterated by an H-D exchange reaction under conditions of 1% NaOD/D(2)O on heating. With a condensation reaction between the D-labeled nicotinic acid derivative and N-hydroxysuccinimide with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, the nicotinoylating agents, 1-(6-methyl[D(3)]nicotinoyloxy)succinimide (2c) and 1-(2,6-dimethyl[D(6)]nicotinoyloxy)succinimide (2f) were prepared. Both D-labeled nicotinoylating agents and their unlabeled counterparts quantitatively modified the N-terminal of protein.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsumoto
authors.forename: Chie
authors.initials: C
authors.lastname: Murata
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
authors.forename: Ryo
authors.initials: R
authors.lastname: Taguchi
authors.forename: Kohfuku
authors.initials: K
authors.lastname: Kohda
mesh_terms: D001920:Bradykinin / Q000032:analysis*; D003903:Deuterium / Q000032:analysis / Q000138:chemical synthesis*; D009539:Nicotinic Acids / Q000032:analysis / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000032:analysis*
chemical_list: D009539:Nicotinic Acids; D010446:Peptide Fragments; C008473:methyl nicotinate; D003903:Deuterium; D001920:Bradykinin