Gene polymorphisms in tumor necrosis factor locus and waist-hip ratio in obese Koreans.
BACKGROUND
The study was designed to investigate the association among tumor necrosis factor-beta (TNFbeta)+252A/G, TNF-alpha (TNFalpha)-308G/A polymorphisms and anthropometric parameters related to obesity in Korean obese subjects.


METHODS
The study included 152 obese healthy subjects (BMI>or=25 kg/m(2), range 25.0-40.4, age range 15-40 years) and 82 non-obese controls (BMI<25 kg/m(2), age range 15-40 years). Total fat mass and percent body fat were determined by dual-energy X-ray absorptiometry (DEXA). Genomic DNA was extracted and used for NcoI restriction fragment length polymorphism (RFLP)-based genotyping of TNF.


RESULTS
No differences were observed for allelic and genotype frequencies between obese and non-obese subjects. Also, no association of TNF polymorphisms was observed with body mass index (BMI) for genotype in obese subjects. In addition, age, percent body fat, BMI and cholesterol concentrations did not differ from TNF genotypes. However, waist-to-hip ratio (WHR) was significantly decreased in subjects with TNFalpha A/TNFbeta G haplotype compared with other haplotypes carriers (0.92+/-0.03 vs. 0.94+/-0.06, P=0.005).


CONCLUSIONS
These results suggest that TNF polymorphisms at position -308 and +252 are not a significant factor for BMI, but affect the WHR in obese Koreans.
Um
Jae-Young
JY
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701, Seoul, South Korea.
Park
Jae-Hwang
JH
Kim
Hyung-Min
HM
D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics*; D001824:Body Constitution / Q000235:genetics*; D015992:Body Mass Index; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007723:Korea; D009765:Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha