Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples.
Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized. Both conjugate-coated ELISAs are based on monoclonal antibodies (MAbs) of different specificity and homologous protein conjugates. Effects of several physicochemical factors (ionic strength, pH, Tween-20 and Bovine serum albumin (BSA) concentrations) and solvents (methanol, ethanol, acetone and N,N'-dimethylformamide) on the performance of the assays were studied and optimized. For the DDT-selective assay, the sensitivity, estimated as the I(50) value, was 0.6 microg/l, with a linear working range between 0.1 and 2 microg/l and a limit of detection of 0.06 microg/l. For the DDT group-selective assay, the sensitivity was 0.2 microg/l, with a linear working range between 0.07 and 1 microg/l and a limit of detection of 0.04 microg/l. CL-ELISAs were four times more sensitive compared to colorimetric ELISAs. Finally, both immunoassays were applied to the detection of DDT and group of DDT-related compounds in spiked real water, soil and food samples.
Botchkareva
Alexandra E
AE
Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119899 Moscow, Russia.
Eremin
Sergei A
SA
Montoya
Angel
A
Manclús
Juan J
JJ
Mickova
Barbora
B
Rauch
Pavel
P
Fini
Fabiana
F
Girotti
Stefano
S
D003429:Cross Reactions; D003634:DDT / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D012987:Soil / Q000032:analysis; D014867:Water / Q000032:analysis
D012987:Soil; D014867:Water; D003634:DDT