Ca2+-ATPase isoforms are expressed in neuroprotection in rat, but not human, neurons.
Glutamate excitotoxicity has been implicated in neuronal death and damage in many neurodegenerative disorders. The potential neuroprotective role of the plasma membrane calcium ATPase (PMCA) and the NMDA receptor were investigated in rat and human brain neurons after a glutamate insult. Investigation of potential mechanisms of neuronal survival revealed that surviving rat cerebellar granule cells expressed the mRNA of new PMCA isoforms 2b and 2c. There was no observable change in expression of PMCA isoforms or NMDA receptor NR2 subtypes in human cortical neurons. This study shows that subsets of rat and human neurons are resistant to glutamate-induced excitotoxicity and the mechanisms employed to enable survival differ between rat and human neurons.
Wanigasekara
Yewlan
Y
Faculty of Pharmacy, School of Biological Sciences, University of Sydney, NSW, Autralia.
Armati
Patricia J
PJ
Roufogalis
Basil D
BD
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis* / Q000235:genetics; D027682:Cation Transport Proteins; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D018698:Glutamic Acid / Q000633:toxicity; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D053499:Plasma Membrane Calcium-Transporting ATPases; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D027682:Cation Transport Proteins; D007527:Isoenzymes; D018698:Glutamic Acid; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases