Tempero-spatial dissociation between the expression of Fas and apoptosis after coronary occlusion.
OBJECTIVE
To explore the role of Fas in cardiomyocytic apoptosis induced by ischaemia through determining the histological relation between Fas expression and apoptosis in rat myocardium during ischaemia/infarction.


METHODS
The myocardial ischaemia model was produced by ligating the left coronary artery in Sprague-Dawley rats. The rats were killed from 10 minutes to seven days after surgery. Apoptotic myocardial cells were detected by the in situ terminal deoxynucleotidyl transferase mediated nick end labelling method, and the expression of Fas by immunohistochemistry and western blotting.


RESULTS
Cardiomyocytic apoptosis appeared from three to 36 hours after ischaemia. The expression of Fas could be detected by western blot from before surgery to seven days of ischaemia. Apoptosis and the expression of Fas in the cardiomyocytes appeared in different regions of the myocardium: apoptosis in the ischaemic region, Fas in the regions surrounding ischaemic myocardium.


CONCLUSIONS
These results suggest that there is a tempero-spatial dissociation between the expression of Fas and apoptosis after coronary occlusion. Fas might not directly regulate the apoptosis of cardiomyocytes induced by ischaemia.
Feng
Q Z
QZ
Department of Cardiology, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China. fqz301@yahoo.com
Li
T D
TD
Wei
L X
LX
Qiao
X
X
Yi
J
J
Wang
L
L
Yang
T S
TS
D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling; D008297:Male; D023421:Models, Animal; D017202:Myocardial Ischemia / Q000276:immunology / Q000473:pathology*; D009206:Myocardium / Q000276:immunology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019014:fas Receptor / Q000032:analysis*
D015415:Biomarkers; D019014:fas Receptor