Acute-phase-dependent binding affinity of C/EBPbeta from the nuclear extract and nuclear matrix towards the hormone response element of the alpha2-macroglobulin gene in rat hepatocytes.
Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied. By Western and South-Western blot analysis we have shown the presence of C/EBPbeta in the examined nuclear fractions as well as its increased binding affinity to the examined gene sequence during the acute-phase response. The results suggest that both nuclear protein fractions could participate in the transcriptional regulation of the alpha2-macroglobulin gene in the rat hepatocytes.
Bogojević
D
D
Molecular Biology Laboratory, Institute for Biological Research, 29 Novembra 142, 11000 Belgrade, Serbia and Montenegro. dekana@ibiss.bg.ac.yu
Mihailović
M
M
Petrović
M
M
Dinić
S
S
Ivanović-Matić
S
S
Poznanović
G
G
D000210:Acute-Phase Reaction / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D015530:Nuclear Matrix / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism*; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000276:immunology / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; C497251:Ddit3 protein, rat; D014157:Transcription Factors; D000511:alpha-Macroglobulins; D051742:Transcription Factor CHOP; D014425:Turpentine