[Protective effects of shenmai injection on DPPH free radical-induced injuries in isolated hearts in rabbits].
OBJECTIVE
To examine the protective effects of shenmai injection (SM) on DPPH free radical-induced myocardial injury in isolated hearts in rabbits.


METHODS
The isolated rabbit hearts were perfused in a Langendorff model. The left ventricular pressure (LVP), the first derivative of LVP (+dp/dtmax), left ventricular end-diastolic pressure (LVEDP) and heart rate(HR) were recorded. Coronary flow (CF) and creatine Kinase (CK) of coronary issue were measured.


RESULTS
Perfusion with DPPH (0.25 mumol/L) for 5 min caused a significant impairment of cardiac function, as shown by a decrease in LVP and +dp/dtmax and an increase in LVEDP as well as the increased release of CK. Pretreatment with shenmai injection (1:320) for 10 min significantly improved the cardiac function and decreased the release of CK.


CONCLUSIONS
Shenmai injection protects against the myocardial injury induced by free radical (DPPH) in isolated rabbit hearts.
Li
Nian-sheng
NS
Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.
Xiong
Xiao-ming
XM
Liu
Li-ying
LY
D000818:Animals; D001713:Biphenyl Compounds; D003326:Coronary Circulation / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006834:Hydrazines; D066298:In Vitro Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D010851:Picrates; D011817:Rabbits
D001713:Biphenyl Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; C004931:1,1-diphenyl-2-picrylhydrazyl; D003402:Creatine Kinase