title: Determination of paeonol in rat plasma by high-performance liquid chromatography and its application to pharmacokinetic studies following oral administration of Moutan cortex decoction.
abstract: Quantification of paeonol, the principal bioactive component of Moutan cortex, in rat plasma following oral administration of Moutan cortex decoction was achieved by using a simple and sensitive high-performance liquid chromatographic method. The calibration curves for paeonol were linear in both the low (25-200 ng/mL) and the high concentration range (200-4000 ng/mL) with r(2) values of 0.9928 and 0.9993, respectively. The coefficients of variation of intra- and inter-day assays were 14.36, 6.52, 1.76, 1.25, 5.36, 3.30 and 1.42% and 12.70, 1.19, 2.98, 1.91, 1.75, 1.78 and 0.96% at concentrations of 25, 50, 100, 200, 500, 1000 and 2000 ng/mL, respectively. The recoveries of paeonol from rat plasma were found to be 101.9, 104.5, 105.4 and 101.2% for concentrations of 50, 500, 1000 and 2000 ng/mL, respectively. The paeonol plasma concentrations were fitted to two-compartment model with fi rst order absorption. The mean terminal half-lives (t(1/2)) of paeonol was 80.9 min.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Xinan
authors.initials: X
authors.lastname: Wu
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
authors.forename: Zhide
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000098:Acetophenones / Q000097:blood* / Q000493:pharmacokinetics; D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000098:Acetophenones; D004365:Drugs, Chinese Herbal; C013638:paeonol