title: Pseudoalteromonas sagamiensis sp. nov., a marine bacterium that produces protease inhibitors.
abstract: A marine bacterium producing protease inhibitors was isolated from neritic sea water and was studied phenotypically, genotypically and phylogenetically. This bacterium (strain B-10-31(T)) produced three types of protease inhibitor, namely, marinostatin, monastatin and leupeptin, which were considerably different in terms of their chemical structure and properties. Strain B-10-31(T) was a rod-shaped, non-spore-forming, Gram-negative, strictly aerobic bacterium that was motile by means of one polar flagellum. The strain required Na(+) for growth and exhibited optimal growth at 27 degrees C, pH 8.0 and 2 % (w/v) NaCl. It utilized various substrates, such as D-glucose, maltose, maltotriose, N-acetylglucosamine, L-threonine, L-serine, L-arginine, L-proline, L-alpha-alanine and L-glutamate, as the sole energy source. Ubiquinone-8 was the major respiratory quinone. The major fatty acids were C(16 : 0), C(16 : 1) omega7c, C(16 : 1) omega9c and C(18 : 1) omega7c. The G+C content of the DNA of strain B-10-31(T) was 42.0 mol%. Phylogenetic analysis, based on 16S rDNA sequences, showed that the strain clustered in the gamma-Proteobacteria. The aerobic marine bacterium Pseudoalteromonas bacteriolytica was the species most closely related to the new isolate (90.4 % 16S rDNA sequence similarity); other described species in the gamma-Proteobacteria cluster showed low levels of sequence similarity with strain B-10-31(T) (<90 %). Based on the above results, it is proposed that the novel marine bacterium should be classified as a new species, for which the name Pseudoalteromonas sagamiensis (type strain B-10-31(T)=JCM 11461(T)=DSM 14643(T)) is proposed.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Fisheries, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Imada
authors.forename: Akira
authors.initials: A
authors.lastname: Hiraishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Inamori
authors.forename: Naoko
authors.initials: N
authors.lastname: Hamada
authors.forename: Etsuo
authors.initials: E
authors.lastname: Watanabe
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D011480:Protease Inhibitors / Q000378:metabolism*; D031161:Pseudoalteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D011480:Protease Inhibitors