[Detection of cytomegalovirus infection by polymerase chain reaction in hematopoietic stem cell transplantation recipients].
OBJECTIVE
To evaluate the detection of cytomegalovirus (CMV) by polymerase chain reaction (PCR) for predicting the development of CMV disease.


METHODS
One hundred and thirty one allo-HSCT patients performed in the past 2 years were analyzed retrospectively. PCR-CMV was used to monitor CMV viremia and vireuria once a week after transplantation.


RESULTS
In the dynamic detection, CMV viremia was positive for at least one chance in 89 patients, vireuria did in 99 patients. Thirty-seven patients developed CMV disease with an accumulative incidence of 32.5%. The incidence of CMV disease was 15.6% in plasma CMV-PCR negative group, 31.3% in positive once group, and 47.3% in positive over twice group. There was significant difference among the three groups (P = 0.0126). The incidence of CMV disease was 24.8% in urine CMV-PCR negative group, 43.5% in positive once group, and 33.0% in positive over twice group, being no significant difference among them (P = 0.845). On analysis, viremia could predict the development of CMV disease: the PPV (positive predictive value) is 40.5%, NPV (negative predictive value) is 84.4%, sensitivity is 75.0%, and specificity is 69.2%.


CONCLUSIONS
Detected by CMV-PCR, MCV viremia may predict the development of CMV disease, but MCV vireuria cannot.
Xu
Lan-ping
LP
Institute of Hematology, People's Hospital, Peking University, Beijing 100044, China.
Huang
Xiao-jun
XJ
Guo
Nai-lan
NL
Ren
Han-yun
HY
Zhang
Yao-chen
YC
Lu
Dao-pei
DP
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology; D004279:DNA, Viral / Q000097:blood / Q000652:urine; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous / Q000009:adverse effects
D004279:DNA, Viral