title: Urinary PAH metabolites influenced by genetic polymorphisms of GSTM1 in male hospital incinerator workers.
abstract: Hospital waste incinerator workers are exposed to various pyrolysis products including polycyclic aromatic hydrocarbons (PAHs). We evaluated their exposure by assessing urinary 1-hydroxypyrene glucuronide (1-OHPG), as an internal dose of PAH exposure. The potential effect of genetic polymorphisms of GSTM1/T1 involved in PAH metabolisms was also investigated. Pre- and post-shift samples were collected from 28 hospital incinerator workers. Urinary 1-OHPG was assayed by synchronous fluorescence spectroscopy (SFS) after immunoaffinity purification with the monoclonal antibody 8E11. Genotypes of GSTM1/T1 were assessed by PCR-based methods. Information on smoking habits and use of personal protective equipment were collected by means of a self-administered questionnaire. The Mann-Whitney test was used to compare group means of these biomarkers. Urinary 1-OHPG levels were similar in pre- and post-shift urine samples. The arithmetic mean concentrations of urinary 1-OHPG were 0.16 +/- 0.04 micromol/mol creatinine pre-shift and 0.19 +/- 0.09 micromol/mol creatinine post-shift, but urinary 1-OHPG levels were significantly higher in individuals with the GSTM1 null genotype than with the GSTM1 present genotype (p=0.05, by Mann-Whitney test). Our results suggest that the urinary 1-OHPG levels in hospital waste incinerator workers may be modified by the GSTM1 genotype, but these findings remain to be confirmed in future studies involving larger sample sizes.
authors.affiliation: Department of Preventive Medicine, Seoul National University College of Medicine, Korea.
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Soo-Hun
authors.initials: SH
authors.lastname: Cho
authors.forename: Yun-Chul
authors.initials: YC
authors.lastname: Hong
authors.forename: Kwan-Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Ho-Jang
authors.initials: HJ
authors.lastname: Kwan
authors.forename: Inmi
authors.initials: I
authors.lastname: Choi
authors.forename: Daehee
authors.initials: D
authors.lastname: Kang
mesh_terms: D000328:Adult; D018736:DNA Adducts / Q000032:analysis; D005838:Genotype; D005965:Glucuronates / Q000652:urine*; D005982:Glutathione Transferase / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D017745:Incineration; D019570:Inhalation Exposure; D007723:Korea; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011110:Polymorphism, Genetic; D011481:Protective Clothing; D011721:Pyrenes; D018709:Statistics, Nonparametric; D011795:Surveys and Questionnaires
chemical_list: D018736:DNA Adducts; D005965:Glucuronates; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C093851:1-hydroxypyrene-glucuronide