Evaluation of antiradical capacity by H2O2-hemin-induced luminol chemiluminescence.
This paper describes a screening method for antioxidant potential determination based on luminol/hemin/hydrogen peroxide chemiluminescence. The emission depletion, caused by an antiradical compound added during the chemiluminescence decay, is proportional to the number of reactive species trapped. Therefore, the difference between the areas of the emission decay curves, obtained in the absence and in the presence of the potential antioxidant, is a measure for the antiradical capacity of the sample. The technique has been applied to measure the antiradical capacity of pure compounds and complex mixtures from natural origin, providing reliable results that indicate the method's feasibility.
Bastos
Erick L
EL
Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Bloco 12 S, São Paulo, SP, 05508-900, Brazil.
Romoff
Paulete
P
Eckert
Camila R
CR
Baader
Wilhelm J
WJ
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D006427:Hemin / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000975:Antioxidants; D008165:Luminol; D006427:Hemin; D006861:Hydrogen Peroxide