[Expression of midkine and its relationship with HBV infection in hepatocellular carcinomas].
OBJECTIVE
To investigate expression of midkine (MK) in hepatocellular carcinoma (HCC) and its relationship with HBV infection.


METHODS
Expression of MK mRNA, MK protein and HBV DNA were determined in 62 cases of human HCC and 10 cases of controls with in situ hybridization and immunohistochemistry.


RESULTS
Positive rates of MK mRNA and MK protein in carcinoma tissue were 74.2% and 75.8% respectively, which were higher than in paratumorous liver tissue and normal controls (P < 0.01). Positive rate of HBV DNA in the nuclei of carcinoma tissue (62.9%) were higher than in paratumorous liver tissue (6.5%) (P < 0.01). There was a significant relationship between expression of MK mRNA and MK protein in the carcinoma tissue and HBV DNA in the nuclei of carcinoma tissue.


CONCLUSIONS
HCC overexpresses MK at the mRNA and protein level. The overexpression of the MK protein might be correlated with the existence of HBV DNA in the nuclei of carcinoma tissue.
Dai
Li-cheng
LC
Department of Infection, Huzhou Central Hospital, Huzhou 313000, China.
Yao
Xing
X
Lu
Yong-liang
YL
Ping
Jin-liang
JL
Zhang
Bu-wen
BW
Ni
Can-rong
CR
D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000378:metabolism / Q000821:virology; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D016207:Cytokines; D004279:DNA, Viral / Q000032:analysis; D006509:Hepatitis B / Q000150:complications; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000209:etiology* / Q000378:metabolism / Q000821:virology; D000078792:Midkine; D012333:RNA, Messenger / Q000032:analysis
D002352:Carrier Proteins; D016207:Cytokines; D004279:DNA, Viral; D012333:RNA, Messenger; D000078792:Midkine