[Effect of endotoxin-induced uveitis on GATA-3 expression and ACAID development].
OBJECTIVE
To investigate the changes of the GATA-3 expression and development of anterior chamber associated immune deviation (ACAID) after anterior chamber (AC) injection of interphotoreceptor retinoid binding protein (IRBP) under ocular inflammation.


METHODS
ACAID was induced by injection of IRBP into the AC of 30 Spar-Dawley (SD) rats. Then the animals were divided into -4 days group, -24 hours group, 0 hour group, 3 days group, and 7 days group according to the lipopolysaccharide (LPS) injection 4 days and 24 hours before, or 0 hour, 3 days and 7 days after IRBP inoculation respectively. 6 rats were used as controls 8 days after IRBP injection, Serum interleukin-4 (IL-4) was evaluated to count the development of ACAID; Western blot and RT-PCR were used to determine the protein and mRNA levels of GATA-3 expression.


RESULTS
In -24 hours group and 0 hour group, the ocular inflammation reached a maximum 24 hours after LPS injection; on 8 days after IRBP inoculation, serum IL-4 couldn't be detected and GATA-3 expression were not changed both on mRNA and protein levels compared with control group. In -4 days group, the ocular inflammation was subsided gradually 24 hours and disappeared 96 hours after LPS injection; serum IL-4 and GATA-3 expression were significantly elevated 8 days after IRBP injection. In 7 days group, the serum IL-4 and GATA-3 expression in spleen increased 8 days after IRBP inoculation.


CONCLUSIONS
In ocular inflammation, the up-regulation of GATA-3 expression is inhibited and ACAID development is blocked after antigen was injected into anterior chamber. Once GATA-3 is up-regulated, LPS injection cannot affect ACAID development.
Fu
Tao
T
Tongren Ophthalmic Center, Capital University of Medical Sciences, Beijing 100730, China.
Yang
Pei-zeng
PZ
Huang
Xiang-kun
XK
Zheng
Hu-ling
HL
Zhou
Hong-yan
HY
Zhong
Hua-hong
HH
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D005786:Gene Expression Regulation; D015847:Interleukin-4 / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012177:Retinol-Binding Proteins / Q000276:immunology*; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics*; D014605:Uveitis / Q000378:metabolism*; D000099214:Retinol-Binding Proteins, Interstitial
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D015847:Interleukin-4; D008070:Lipopolysaccharides; D012177:Retinol-Binding Proteins; D015534:Trans-Activators; D000099214:Retinol-Binding Proteins, Interstitial