title: More efficient mobilisation of peripheral blood stem cells with HiDAC+AMSA+G-CSF than with mini-ICE+G-CSF in patients with AML.
abstract: We have compared the efficacy of two PBSC mobilisation regimens, mini-ICE+filgrastim (second consolidation) and HiDAC+AMSA+filgrastim (third consolidation), in two consecutive cohorts of patients with AML CR1 receiving treatment according to a joint protocol. Group A: 18 patients, aged 41 (21-65) years, were mobilised with mini-ICE (idarubicin 8 mg/m(2)+cytarabine 800 mg/m(2)+etoposide 150 mg/m(2) days 1-3) followed by filgrastim 300-480 microg once daily s.c. from day 11 after start of chemotherapy. Only four patients reached >5 CD34+ cells/microl blood (B-CD34+) and were able to undergo leukaphereses. Two out of 18 (11%) reached the defined target of >/=2.0 x 10(6) CD34+ cells/kg after 1-3 leukaphereses. Group B: 20 patients, aged 50 (29-67) years, received HiDAC+AMSA (cytarabine 3 g/m(2) b.i.d. days 1, 3, 5+amsacrine 150 mg/m(2) q.d. days 2, 4) followed by filgrastim at a similar dose starting on day 7. A total of 18 patients reached B-CD34+ >5/microl and underwent PBSC harvesting, starting on day 23 (14-29) and yielding 4.0 (0.9-21) x 10(6) CD34+ cells/kg. Of 20 patients, 17 (85%) reached the defined target of >/=2.0 x 10(6) CD34+ cells/kg after 1-3 leukaphereses. We conclude that HiDAC+AMSA+G-CSF - in contrast to mini-ICE+G-CSF - is an efficient regimen for mobilising PBSC in patients with AML CR1.
authors.affiliation: Department of Hematology, University Hospital, Uppsala, Sweden. martin.hoglund@medsci.uu.se
authors.forename: M
authors.initials: M
authors.lastname: Höglund
authors.forename: M
authors.initials: M
authors.lastname: Brune
authors.forename: B
authors.initials: B
authors.lastname: Sallerfors
authors.forename: T
authors.initials: T
authors.lastname: Ahlgren
authors.forename: R
authors.initials: R
authors.lastname: Billström
authors.forename: M
authors.initials: M
authors.lastname: Hedenus
authors.forename: B
authors.initials: B
authors.lastname: Markevärn
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
authors.forename: B
authors.initials: B
authors.lastname: Simonsson
authors.forename: D
authors.initials: D
authors.lastname: Stockelberg
authors.forename: A
authors.initials: A
authors.lastname: Wahlin
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000677:Amsacrine / Q000008:administration & dosage / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000627:therapeutic use; D001772:Blood Cell Count; D053456:Caspase 14; D020169:Caspases / Q000008:administration & dosage / Q000494:pharmacology*; D015331:Cohort Studies; D003131:Combined Modality Therapy; D003561:Cytarabine / Q000008:administration & dosage / Q000494:pharmacology*; D005047:Etoposide / Q000008:administration & dosage; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D015255:Idarubicin / Q000008:administration & dosage / Q000009:adverse effects; D007937:Leukapheresis; D007951:Leukemia, Myeloid / Q000097:blood* / Q000188:drug therapy / Q000628:therapy; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D011994:Recombinant Proteins; D014182:Transplantation, Autologous
chemical_list: D011994:Recombinant Proteins; D000677:Amsacrine; D003561:Cytarabine; D016179:Granulocyte Colony-Stimulating Factor; D005047:Etoposide; C506446:CASP14 protein, human; D053456:Caspase 14; D020169:Caspases; D000069585:Filgrastim; D015255:Idarubicin