[Study on invasion and metastasis related factors in differentiation of SMMC-7721 cells induced by matrine].
OBJECTIVE
To study the inhibition effect on invasion and metastasis related factors in differentiation of SMMC-7721 cells induced by matrine.


METHODS
Human hepatoma cell lines SMMC-7721 were cultured in vitro and treated by 0.5 g/L, 0.8 g/L, 1.0 g/L matrine. Then the cell growth curve was protracted, the cell form was observed by light microscope, the invasion and metastasis related factors were measured with cell adhesion test, cell migration test and cell invasion test. The data was collected and analyzed with statistical methods to evaluate roundly the inducing differentiation function of matrine.


RESULTS
Treated by 0.8 g/L, 1.0 g/L matrine, the proliferation of SMMC-7721 was inhibited markedly, and the malignant cell form tended to normal differentiation. It was also found that matrine could inhibit cell invasion and metastasis related factors, including adhesive ability to FN, migrative ability through the polycarbonate filter, and invasive ability through matrigel.


CONCLUSIONS
Matrine is a good differentiation inducer, it can not only inhibit the cell proliferation and induce the cell form differentiation, but also significantly inhibit the invasion and metastasis ability of SMMC-7721 through several steps, involved cell adhesion, migration and invasion.
Wang
Yong
Y
Second Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009.
Peng
Chenghong
C
Zhang
Guoping
G
Liu
Yingbin
Y
Li
Hong
H
Shan
Jianzhen
J
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines; D029909:Sophora / Q000737:chemistry; D014407:Tumor Cells, Cultured; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D000093842:Matrines