title: Instrumentation for multi-modal spectroscopic diagnosis of epithelial dysplasia.
abstract: Reflectance and fluorescence spectroscopies have shown great promise for early detection of epithelial dysplasia. We have developed a clinical reflectance spectrofluorimeter for multimodal spectroscopic diagnosis of epithelial dysplasia. This clinical instrument, the FastEEM, collects white light reflectance and fluorescence excitation-emission matrices (EEM's) within a fraction of a second. In this paper we describe the FastEEM instrumentation, designed for collection of multi-modal spectroscopic data. We illustrate its performance using tissue phantoms with well defined optical properties and biochemicals of known fluorescence properties. In addition, we discuss our plans to develop a system that combines a multi-spectral imaging device for wide area surveillance with this contact probe device.
authors.affiliation: G.R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Ave., Bldg 6-014, Cambridge, MA 02139, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Tunnell
authors.forename: A E
authors.initials: AE
authors.lastname: Desjardins
authors.forename: L
authors.initials: L
authors.lastname: Galindo
authors.forename: I
authors.initials: I
authors.lastname: Georgakoudi
authors.forename: S A
authors.initials: SA
authors.lastname: McGee
authors.forename: J
authors.initials: J
authors.lastname: Mirkovic
authors.forename: M G
authors.initials: MG
authors.lastname: Mueller
authors.forename: J
authors.initials: J
authors.lastname: Nazemi
authors.forename: F T
authors.initials: FT
authors.lastname: Nguyen
authors.forename: A
authors.initials: A
authors.lastname: Wax
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: R R
authors.initials: RR
authors.lastname: Dasari
authors.forename: M S
authors.initials: MS
authors.lastname: Feld
mesh_terms: D002277:Carcinoma / Q000175:diagnosis*; D004848:Epithelium / Q000473:pathology*; D005336:Fiber Optic Technology; D006801:Humans; D055095:Optics and Photonics; D019047:Phantoms, Imaging; D012984:Software; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013057:Spectrum Analysis / Q000295:instrumentation*