title: On/off switch mediated by Exo+ polymerases: experimental analysis for its physiological and technological implications.
abstract: The potential physiological role and technological application of the premature termination of DNA polymerization through the off-switch of exo+ polymerases were studied using 3' phosphorothioate-modified or unmodified primers with single base mismatch distal to the 3' terminus. With exonuclease-digestible unmodified primers, a gradient premature termination of DNA polymerization was observed when amplified with exo+ polymerases. With 3' allele specific phosphorothioate-modified primers, an efficient off-switch effect occurred in the discrimination of a single nucleotide polymorphism when directly using genomic DNA. Clearly, the off-switch of exo+ polymerases is useful in biomedical research.
authors.affiliation: Genomapping Inc., Tianjin, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Lin-Ling
authors.initials: LL
authors.lastname: Chen
authors.forename: Zi-Fen
authors.initials: ZF
authors.lastname: Guo
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Peng
authors.forename: Duan-Fang
authors.initials: DF
authors.lastname: Liao
authors.forename: Kai
authors.initials: K
authors.lastname: Li
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001704:Biopolymers; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism* / Q000502:physiology; D013873:Thionucleotides / Q000737:chemistry
chemical_list: D001704:Biopolymers; D017931:DNA Primers; D013873:Thionucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase