HPLC analysis of some sulphur compounds in saliva: comparison between healthy subjects and periodontopathic patients.
BACKGROUND
Saliva is an easily available biological material that is not commonly analysed in clinical chemistry, while it could give useful information especially in several oral diseases.


METHODS
In this work, the sulphur containing compounds cysteine, cysteinylglycine and glutathione were analysed in saliva of control subjects and periodontopathic subjects by a HPLC method. The detection limit of the method is 0.5, 0.1 and 0.1 micromol/l for cysteine, cysteinylglycine and glutathione, respectively, and it is linear up to 10 mmol/l.


RESULTS
the median values for the control group are 1.2 micromol/l for cysteine and glutathione and 0.4 micromol/l for cysteinylglycine while those of periodontopathic patients are significantly increased (4.4, 2.1 and 11.0 micromol/l for cysteine, cysteinylglycine and glutathione, respectively).
Zappacosta
Bruno
B
Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
Manni
Armando
A
Persichilli
Silvia
S
Scribano
Donata
D
Minucci
Angelo
A
Lazzaro
Daniele
D
De Sole
Pasquale
P
Giardina
Bruno
B
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis / Q000378:metabolism; D004151:Dipeptides / Q000032:analysis / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D013457:Sulfur Compounds / Q000032:analysis* / Q000378:metabolism
D004151:Dipeptides; D013457:Sulfur Compounds; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine