Enhancement of antibody response by one-trial conditioning: contrasting results using different antigens.
New research in conditioned enhancement of antibody response requires a general paradigm effective with different antigens. In this experiment series we applied a one-trial protocol using keyhole limpet hemocyanin immunization as an unconditioned stimulus. Several different conditions were tested. Two different times between conditioning and test trial, two relevant antigen doses and the use of an antigen booster during test trial were investigated. We did not find a conditioned effect in any of the conditions used. In contrast, we found a reliable albeit modest conditioned effect using hen egg lysozyme as unconditioned stimulus. By comparing these and other findings we conclude that the number of conditioning trials is a possible requirement for a more reliable conditioning of antibody response.
Espinosa
Enrique
E
Department of Neuroscience, Cell Physiology Institute, National Autonomous University of Mexico UNAM, Apartado Postal 70-253, Mexico DF 04510, Mexico.
Calderas
Tania
T
Flores-Muciño
Oscar
O
Pérez-García
Georgina
G
Vázquez-Camacho
Ana C
AC
Bermúdez-Rattoni
Federico
F
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D003213:Conditioning, Psychological / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006433:Hemocyanins / Q000276:immunology; D008297:Male; D009113:Muramidase / Q000276:immunology; D015213:Neuroimmunomodulation / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000941:Antigens; D006433:Hemocyanins; C421864:hen egg lysozyme; D009113:Muramidase; C032808:keyhole-limpet hemocyanin