Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.
E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
Cortes-Perez
Naima G
NG
Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L. 66450, Mexico.
Bermúdez-Humarán
Luis G
LG
Le Loir
Yves
Y
Rodriguez-Padilla
Cristina
C
Gruss
Alexandra
A
Saucedo-Cárdenas
Odila
O
Langella
Philippe
P
Montes-de-Oca-Luna
Roberto
R
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D005260:Female; D007114:Immunization / Q000379:methods*; D017045:Lactococcus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000517:prevention & control*
D000954:Antigens, Surface; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16