Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.
Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.
Mayta
Holger
H
Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Gilman
Robert H
RH
Arenas
Fanny
F
Valencia
Teresa
T
Caviedes
Luz
L
Montenegro
Sonia H
SH
Ticona
Eduardo
E
Ortiz
Jaime
J
Chumpitaz
Rosa
R
Evans
Carlton A
CA
Williams
Diana L
DL
D000995:Antitubercular Agents / Q000494:pharmacology / Q000627:therapeutic use; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010568:Peru; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000188:drug therapy
D000995:Antitubercular Agents; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin