title: Coding potential of laboratory and clinical strains of human cytomegalovirus.
abstract: Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 80544, USA.
authors.forename: Eain
authors.initials: E
authors.lastname: Murphy
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.forename: Jane
authors.initials: J
authors.lastname: Grimwood
authors.forename: Jeremy
authors.initials: J
authors.lastname: Schmutz
authors.forename: Mark
authors.initials: M
authors.lastname: Dickson
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jarvis
authors.forename: Gabriele
authors.initials: G
authors.lastname: Hahn
authors.forename: Jay A
authors.initials: JA
authors.lastname: Nelson
authors.forename: Richard M
authors.initials: RM
authors.lastname: Myers
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Shenk
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D004279:DNA, Viral; D005347:Fibroblasts / Q000378:metabolism; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames
chemical_list: D004279:DNA, Viral; D004247:DNA