title: Glial cell and fibroblast cytotoxicity study on 4026-cyclotene photosensitive benzocyclobutene (BCB) polymer films.
abstract: Photosensitive benzocyclobutene (photo-BCB) is a class of polymers with the trade name Cyclotene. The photoimagable property of Cyclotene makes it suitable for the manufacture of microelectronic devices. The motivation behind this study is that we see an exciting application of photo-BCB as substrates in implantable microelectronic biomedical devices due to several desirable properties distinctive from other polymer materials. To our knowledge, however, photo-BCB has never been tested for biomedical implant applications, as evidenced by the lack reported data on its biocompatibility. This study takes the first step towards assessing photo-BCB biocompatibility by evaluating the cytotoxicity and cell adhesion behavior of Cyclotene 4026 coatings exposed to monolayers of glial and fibroblast cells in vitro. It can be concluded from these studies that photo-BCB films deposited on silicon wafers using microfabrication processes did not adversely affect 3T3 fibroblast and T98-G glial cell function in vitro. We also successfully rendered photo-BCB films non-adhesive (no significant fibroblast or glial cell adhesion) with surface immobilized dextran using methods developed for other biomaterials and applications. Future work will further develop prototype photo-BCB microelectrode devices for chronic neural implant applications.
authors.affiliation: Harrington Department of Bioengineering, Arizona State University, PO Box 879709, Tempe, AZ 85287-9709, USA.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Ehteshami
authors.forename: Amarjit
authors.initials: A
authors.lastname: Singh
authors.forename: Gene
authors.initials: G
authors.lastname: Coryell
authors.forename: Stephen
authors.initials: S
authors.lastname: Massia
authors.forename: Jiping
authors.initials: J
authors.lastname: He
authors.forename: Gregory
authors.initials: G
authors.lastname: Raupp
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000633:toxicity*; D046508:Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects*; D011083:Polycyclic Compounds / Q000737:chemistry / Q000633:toxicity*; D011108:Polymers / Q000737:chemistry / Q000633:toxicity*; D019736:Prostheses and Implants
chemical_list: D020099:Coated Materials, Biocompatible; D011083:Polycyclic Compounds; D011108:Polymers; C079040:benzocyclobutene