A BAC-based physical map of the chicken genome.
A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.
Ren
Chengwei
C
Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
Lee
Mi-Kyung
MK
Yan
Bo
B
Ding
Kejiao
K
Cox
Bettye
B
Romanov
Michael N
MN
Price
Jennifer A
JA
Dodgson
Jerry B
JB
Zhang
Hong-Bin
HB
D000818:Animals; D002645:Chickens / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D019295:Computational Biology / Q000379:methods; D020451:Contig Mapping / Q000379:methods*; D016172:DNA Fingerprinting; D016678:Genome