The use of genomics and proteomics to understand oocyte and early embryo functions in farm animals.
Oocyte maturation, a simple and visible phenomenon, is about to be transformed into a complex and not so visible molecular cascade leading to the marking of the following generation. The study of oocyte maturation in mammals is progressively changing towards a more molecular approach. This review addresses the main challenges in the study of RNA extraction and quantification in oocytes and embryos as well as the importance of the mRNA maturation. The identification of specific genes in oocytes and embryos is now possible with the use of powerful tools, such as library analysis or subtractions, DNA array, comparative analysis of databanks from other mammals or animals and two-dimensional gel electrophoresis analysis. Finally, RNA interference is a useful tool for studying gene function by knocking out the activity of specific genes and will be used in oocytes and embryos.
Sirard
M A
MA
Centre de Recherche en Biologie de la Reproduction, Department of Animal Sciences, Laval University, Sainte-Foy, QC, Canada G1K 7P4. marc-andre.sirard@crbr.ulaval.ca
Dufort
I
I
Coenen
K
K
Tremblay
K
K
Massicotte
L
L
Robert
C
C
D000818:Animals; D000829:Animals, Domestic / Q000378:metabolism*; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D030541:Databases, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005820:Genetic Testing; D016678:Genome; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000378:metabolism*; D010060:Ovulation; D020543:Proteome; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012756:Sheep
D020543:Proteome; D012333:RNA, Messenger