Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir.
Classification of strain W3-B1, which was isolated from condensation water in the Russian space laboratory Mir, was investigated by a polyphasic taxonomic approach. Cells of strain W3-B1 were nonmotile, asporogenous, gram-negative slender rods with rounded ends. 16S rRNA gene sequence analysis indicated that organism should be placed in the genus Chryseobacterium. This organism contains menaquinone MK-6 as the predominent isoprenoid quinone and 3-OH iso 17:0 (40%), iso 15:0 (33%) as the major fatty acids. Phylogenetically, the nearest relative of strain W3-B1 is Chryseobacterium meningosepticum with sequence similarity of 98.4%, but DNA-DNA hybridization resulted in similarity values of only 52.3%. The G+C mol% is 34.6 mol%. Based upon results obtained by morphological, biochemical, chemotaxonomic, and molecular methods, strain W3-B1 was clearly distinguishable from other Chryseobacterium species. For these reasons, a novel species of family Flavobacteriaceae is proposed; strain W3-B1(T) (= GTC 862(T) = JCM 11413(T) = DSM 14571(T)) is the type strain.
Li
Ying
Y
Department of Microbiology - Bioinformatics, Regeneration and Advanced Medical Science, Gifu University, Graduate School of Medicine, Gifu, Japan.
Kawamura
Yoshiaki
Y
Fujiwara
Nagatoshi
N
Naka
Takashi
T
Liu
Hongsheng
H
Huang
Xinxiang
X
Kobayashi
Kazuo
K
Ezaki
Takayuki
T
D001482:Base Composition; D045247:Chryseobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018531:Spacecraft; D013171:Spores, Bacterial; D024482:Vitamin K 2 / Q000032:analysis / Q000737:chemistry; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2