title: Transgenic bcl-2 is not sufficient to rescue all hematolymphoid defects in STAT5A/5B-deficient mice.
abstract: OBJECTIVE
  Cytokines bind high-affinity receptors expressed on hematopoietic cells to initiate signaling cascades that regulate differentiation, proliferation, and survival. Previous studies have established a role for STAT5 in transducing survival signals for hematopoietic progenitor cells in response to cytokines.
  
  
  METHODS
  To determine if constitutive expression of a member of the bcl-2 family of anti-apoptotic proteins could compensate for the loss of STAT5, we utilized combinatorial genetics to generate STAT5A/5B-deficient mice expressing a bcl-2 transgene.
  
  
  RESULTS
  Although bcl-2 expression restored peripheral blood counts to normal in STAT5A/5B(-/-) mice, we noted a striking failure of this transgene to correct defects in hematopoietic stem and progenitor cells.
  
  
  CONCLUSIONS
  These data imply important effects of STAT5 in modulating hematopoietic cells in addition to promoting survival per se.
authors.affiliation: Gladstone Institute of Virology and Immunology, Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94141-9100, USA.
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Snow
authors.forename: Ninan
authors.initials: N
authors.lastname: Abraham
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Ma
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Bronson
authors.forename: Mark A
authors.initials: MA
authors.lastname: Goldsmith
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006413:Hematopoietic System / Q000187:drug effects* / Q000254:growth & development; D008208:Lymphatic System / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008894:Milk Proteins; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics; D019076:Transgenes; D017211:Treatment Failure
chemical_list: D004268:DNA-Binding Proteins; D008894:Milk Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D050799:STAT5 Transcription Factor; C494093:Stat5a protein, mouse; D015534:Trans-Activators