A novel HLA-A allele: A*0257.
A novel human leucocyte antigen-A*02 (HLA-A*02) allele was detected by reference strand-mediated conformation analysis (RSCA) of a DNA sample from a Tarahumara individual. Direct sequencing of HLA-A locus polymerase chain reaction products identified a mutation in one of the alleles. Cloning and sequencing confirmed the presence of a new allele, A*0257 which differed from A*0206 by two nucleotides at positions 355 and 362, inducing changes in residues 95 and 97, respectively, within the peptide-binding site. Those changes suggest that allele A*0257 may have resulted from an intralocus recombination event.
García-Ortiz
J E
JE
Departamento de Inmunobiología Molecular, Centro de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Coahuila, Torreón, Coahuila, México.
Cox
S T
ST
Sandoval-Ramirez
L
L
Little
A M
AM
Marsh
S G E
SG
Madrigal
J A
JA
Argüello
J R
JR
D000483:Alleles; D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D015234:HLA-A Antigens