Influence of anticoagulants on the measurement of S100B protein in blood.
OBJECTIVE
Evaluate anticoagulants influence on blood S100B levels.


METHODS
Blood from 18 healthy adult subjects were collected using: no anticoagulants; EDTA; heparin; and citrate. S100B levels were determined using LIA-mat assay.


RESULTS
Heparin and citrate increased S100B levels (p<0.001), whereas EDTA had no effect (p=0.24). Heparin samples were highly (r2=0.97, p<0.001), citrate samples were moderately (r2=0.49, p<0.001), and EDTA samples were not (r2=0.22, p=0.059) correlated with serum samples.


CONCLUSIONS
When anticoagulant is required, heparin should be the primary choice.
Tort
Adriano B L
AB
Departamento de Bioquímica, ICBS, UFRGS, Brazil.
Dietrich
Marcelo O
MO
Gonçalves
Carlos A
CA
Souza
Diogo O
DO
Portela
Luis V C
LV
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*
D000925:Anticoagulants; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human