title: High deleterious genomic mutation rate in stationary phase of Escherichia coli.
abstract: In natural habitats, bacteria spend most of their time in some form of growth arrest. Little is known about deleterious mutations in such stages, and consequently there is limited understanding of what evolutionary events occur. In a deleterious mutation accumulation experiment in prolonged stationary phase of Escherichia coli, about 0.03 slightly deleterious mutations were observed per genome per day. This is over an order of magnitude higher than extrapolations from fast-growing cells, but in line with inferences from observations in adaptive stationary phase mutation experiments. These findings may affect understanding of bacterial evolution and the emergence of bacterial pathogenicity.
authors.affiliation: Microbial Ecology Group, Department of Biosciences, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany. Laurence.Loewe@evolutionary-research.net
authors.forename: Laurence
authors.initials: L
authors.lastname: Loewe
authors.forename: Volker
authors.initials: V
authors.lastname: Textor
authors.forename: Siegfried
authors.initials: S
authors.lastname: Scherer
mesh_terms: D005075:Biological Evolution; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D016680:Genome, Bacterial; D016013:Likelihood Functions; D009154:Mutation; D010641:Phenotype; D012641:Selection, Genetic; D013696:Temperature; D013997:Time Factors