Expression of the murine glutathione S-transferase alpha3 (GSTA3) subunit is markedly induced during adipocyte differentiation: activation of the GSTA3 gene promoter by the pro-adipogenic eicosanoid 15-deoxy-Delta12,14-prostaglandin J2.
The expression of class alpha, micro, and pi glutathione S-transferases (GSTs) has been examined during the adipose conversion of mouse 3T3-L1 cells. The GSTA4, GSTM1, and GSTP1/2 subunits are expressed constitutively in confluent 3T3-L1 cells, and their levels remain essentially unchanged during adipocyte differentiation. By contrast, the GSTA3 subunit is virtually undetectable in confluent 3T3-L1 cells under basal conditions, but its expression is markedly induced during adipose conversion. Inhibition of the 3T3-L1 adipogenic program demonstrated that GSTA3 expression is associated specifically with acquisition of the adipocytic phenotype. Reporter gene assays demonstrated that the mouse GSTA3 5(')-upstream region is transcriptionally activated by 15-deoxy-Delta(12,14)-prostaglandin J(2) through an antioxidant response element, suggesting that this pro-adipogenic eicosanoid may be involved in regulating GSTA3 expression during adipogenesis. These data suggest a previously unrecognised role for GSTs in mouse adipocytes.
Jowsey
Ian R
IR
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK. i.r.jowsey@dundee.ac.uk
Smith
Stephen A
SA
Hayes
John D
JD
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000201:enzymology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D015777:Eicosanoids / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000235:genetics* / Q000378:metabolism*; D021122:Protein Subunits; D015533:Transcriptional Activation / Q000502:physiology
C097240:15-deoxy-delta(12,14)-prostaglandin J2; D015777:Eicosanoids; D021122:Protein Subunits; C000594000:GSTA3 protein, mouse; D005982:Glutathione Transferase; D015230:Prostaglandin D2