Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae.
Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member of the Caulimoviridae family. All characteristic domains conserved in plant pararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.
Stavolone
Livia
L
Friedrich Miescher Institute, Basel, Switzerland.
Ragozzino
Antonio
A
Università di Napoli, via Università, 80055 Portici (NA), Italy.
Hohn
Thomas
T
Friedrich Miescher Institute, Basel, Switzerland.
D000595:Amino Acid Sequence; D017796:Caulimovirus / Q000145:classification / Q000235:genetics*; D032301:Cestrum / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins