Induction of interleukin-8 gene expression by black-pigmented Bacteroides in human pulp fibroblasts and osteoblasts.
OBJECTIVE
To investigate the effect of black-pigmented Bacteroides on the expression of interleukin (IL)-8 gene in human pulp fibroblasts and osteoblasts.


METHODS
The supernatants of Porphyromonas endodontalis, P. gingivalis and Prevotella intermedia were used to evaluate IL-8 gene expression in human pulp fibroblasts and osteoblasts. The levels of mRNAs were measured by the quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis.


RESULTS
Investigations of the time-dependence of IL-8 mRNA expression in black-pigmented Bacteroides-treated pulp fibroblasts and osteoblasts revealed a rapid accumulation of the transcript after 2 h of exposure, and remained elevated throughout the 24-h incubation period. In addition, IL-8 mRNA gene expression was also found in human osteoblasts stimulated with black-pigmented Bacteroides. However, black-pigmented Bacteroides was found to be more effective in the induction of IL-8 mRNA gene expression in osteoblasts than in pulp fibroblasts (P < 0.05).


CONCLUSIONS
Black-pigmented Bacteroides are capable of amplifying the local immune response and promoting pulpal/periapical tissue inflammation by stimulating pulp fibroblasts and osteoblasts to express IL-8.
Yang
L-C
LC
Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
Huang
F-M
FM
Lin
C-S
CS
Liu
C-M
CM
Lai
C-C
CC
Chang
Y-C
YC
D000704:Analysis of Variance; D001438:Bacteroidaceae / Q000235:genetics*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003782:Dental Pulp / Q000276:immunology* / Q000382:microbiology; D005347:Fibroblasts / Q000276:immunology* / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D010006:Osteoblasts / Q000276:immunology* / Q000382:microbiology; D045202:Porphyromonas endodontalis / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000235:genetics; D018713:Prevotella intermedia / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
D016209:Interleukin-8; D012333:RNA, Messenger