title: Replication timing of the human genome.
abstract: We have developed a directly quantitative method utilizing genomic clone DNA microarrays to assess the replication timing of sequences during the S phase of the cell cycle. The genomic resolution of the replication timing measurements is limited only by the genomic clone size and density. We demonstrate the power of this approach by constructing a genome-wide map of replication timing in human lymphoblastoid cells using an array with clones spaced at 1 Mb intervals and a high-resolution replication timing map of 22q with an array utilizing overlapping sequencing tile path clones. We show a positive correlation, both genome-wide and at a high resolution, between replication timing and a range of genome parameters including GC content, gene density and transcriptional activity.
authors.affiliation: The Welcome Trust Sanger Institute, Welcome Genome Campus, Cambridge, UK.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Woodfine
authors.forename: Heike
authors.initials: H
authors.lastname: Fiegler
authors.forename: David M
authors.initials: DM
authors.lastname: Beare
authors.forename: John E
authors.initials: JE
authors.lastname: Collins
authors.forename: Owen T
authors.initials: OT
authors.lastname: McCann
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Young
authors.forename: Silvana
authors.initials: S
authors.lastname: Debernardi
authors.forename: Richard
authors.initials: R
authors.lastname: Mott
authors.forename: Ian
authors.initials: I
authors.lastname: Dunham
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Carter
mesh_terms: D001482:Base Composition; D002478:Cells, Cultured; D002892:Chromosomes, Human, Pair 22; D004261:DNA Replication; D015870:Gene Expression; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016196:S Phase / Q000235:genetics