Identification of a human erythroid progenitor cell population which expresses the CD34 antigen and binds the plant lectin Ulex europaeus I.
Two and three color flow cytometry of normal human bone marrow was used to identify CD34+ progenitor cells and examine their binding to the plant lectin Ulex europaeus I (Ulex). In normal bone marrow, 48.48 +/- 17.4% of the CD34+ cells bind to Ulex. Two color flow cytometry was used to sort CD34 + cells, and subsets of CD34+ cells, CD34+ Ulex+ and CD34+ Ulex-. These populations were sorted into colony assays to assess myeloid (CFU-GM) and erythroid (BFU-E) progenitors. The CD34+ Ulex+ subset was 84 +/- 14% BFU-E colonies (mean +/- S.D.) and had the highest cloning efficiency of 28 +/- 13%. Three color analysis of CD34+ Ulex+ cells showed staining with other erythroid (CD71, GlyA) antibodies and lack of stain. ing with myeloid (CD13, CD45RA) antibodies. These studies confirmed the erythroid characteristics of this subpopulation.
Unverzagt
K L
KL
Immunotherapy Division, Biotech Group, Baxter Healthcare Corporation, Round Lake Illinois 60073, USA.
Martinson
J
J
Lee
W
W
Stiff
P J
PJ
Williams
S
S
Bender
J G
JG
D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D018952:Antigens, CD34 / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D019070:Cell Lineage / Q000276:immunology*; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism*; D015203:Reproducibility of Results
D000906:Antibodies; D018952:Antigens, CD34; D015415:Biomarkers; D037121:Plant Lectins; C036850:Ulex europaeus lectins