Gestation stage-specific oxidative deoxyribonucleic acid damage from sidestream smoke in pregnant rats and their fetuses.
Transplacental exposure to environmental tobacco smoke (ETS) is a possible cancer risk factor in offspring. The authors exposed pregnant Sprague-Dawley rats to a relevant dose of ETS (1 mg/m3) from gestation day 4 to days 16 or 21. They then assayed tissues for levels of 8-oxo-2'-deoxyguanosine (8-oxo-dG), a marker of oxidative deoxyribonucleic acid damage. ETS exposure ending on gestation day 16 resulted in statistically significant increases in 8-oxo-dG in maternal liver and kidney and in fetal kidney. On gestation day 21, there were significant 8-oxo-dG increases in fetal liver and brain. These gestational stage- and tissue-specific increases of 1.2- to 1.4-fold are similar to the putative relative increases in risk of human cancers related to ETS.
Maciag
Anna
A
Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA.
Bialkowska
Aneta
A
Espiritu
Imelda
I
Powell
Douglas
D
Alvord
W Gregory
WG
Kasprzak
Kazimierz S
KS
Anderson
Lucy M
LM
Witschi
Hanspeter R
HR
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D005333:Fetus / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000196:embryology; D008099:Liver / Q000187:drug effects; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D014028:Tobacco Smoke Pollution; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine