Effect of ribose supplementation on resynthesis of adenine nucleotides after intense intermittent training in humans.
The effect of oral ribose supplementation on the resynthesis of adenine nucleotides and performance after 1 wk of intense intermittent exercise was examined. Eight subjects performed a random double-blind crossover design. The subjects performed cycle training consisting of 15 x 10 s of all-out sprinting twice per day for 7 days. After training the subjects received either ribose (200 mg/kg body wt; Rib) or placebo (Pla) three times per day for 3 days. An exercise test was performed at 72 h after the last training session. Immediately after the last training session, muscle ATP was lowered (P < 0.05) by 25 +/- 2 and 22 +/- 3% in Pla and Rib, respectively. In both Pla and Rib, muscle ATP levels at 5 and 24 h after the exercise were still lower (P < 0.05) than pretraining. After 72 h, muscle ATP was similar (P > 0.05) to pretraining in Rib (24.6 +/- 0.6 vs. 26.2 +/- 0.2 mmol/kg dry wt) but still lower (P < 0.05) in Pla (21.1 +/- 0.5 vs. 26.0 +/- 0.2 mmol/kg dry wt) and higher (P < 0.05) in Rib than in Pla. Plasma hypoxanthine levels after the test performed at 72 h were higher (P < 0.05) in Rib compared with Pla. Mean and peak power outputs during the test performed at 72 h were similar (P > 0.05) in Pla and Rib. The results support the hypothesis that the availability of ribose in the muscle is a limiting factor for the rate of resynthesis of ATP. Furthermore, the reduction in muscle ATP observed after intense training does not appear to be limiting for high-intensity exercise performance.
Hellsten
Y
Y
Human Physiology, Copenhagen Muscle Research Centre, Institute for Excersize and Sport Science, DK-2100 Copenhagen, Denmark. yhellsten@aki.ku.dk
Skadhauge
L
L
Bangsbo
J
J
D000227:Adenine Nucleotides / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D001642:Bicycling; D001786:Blood Glucose / Q000032:analysis; D002395:Catecholamines / Q000097:blood; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004311:Double-Blind Method; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D019271:Hypoxanthine / Q000097:blood; D007291:Inosine Monophosphate / Q000378:metabolism; D007328:Insulin / Q000097:blood; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010806:Physical Education and Training; D010807:Physical Endurance / Q000502:physiology*; D012266:Ribose / Q000494:pharmacology*; D014527:Uric Acid / Q000097:blood
D000227:Adenine Nucleotides; D001786:Blood Glucose; D002395:Catecholamines; D007328:Insulin; D010725:Phosphocreatine; D007291:Inosine Monophosphate; D014527:Uric Acid; D019271:Hypoxanthine; D019344:Lactic Acid; D012266:Ribose; D000255:Adenosine Triphosphate; D006003:Glycogen