[Experimental study on expression of GM-CSF from human endothelial cells and monocytes induced by total saponins of panax ginseng].
OBJECTIVE
To study the effect of total saponins of Panax ginseng (TSPG) on the expression of granulocytemacrophage colony-stimulating factor (GM-CSF) from human endothelial cells and monocytes and the relationship between TSPG and human granulocytopoiesis and monocytopoiesis modulation.


METHODS
Adopting the hematopoietic progenitor cells culture in vitro, hematopoietic growth factor biological assay, immunocytochemistry and nucleic acid in situ hybridization, the GM-CSF expression in the endothelial cells and monocytes were detected.


RESULTS
The conditioned cultural media of endothelial and monocytes induced and prepared by TSPG, could significantly promote the proliferation and differentiation of human colony forming unit-granulocyte macrophage (CFU-GM), and enhance the protein and mRNA expression of GM-CSF in endothelial cells and monocytes.


CONCLUSIONS
TSPG could possibly through direct or indirect route, promote hematopoietic, induce endothelial cells and monocytes in the microenvironment to synthetize and secrete GM-CSF, so as to further promote the proliferation and differentiation of human CFU-GM.
Chen
Di
D
Department of Physiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016.
Wang
Sha-li
SL
Wang
Ya-ping
YP
D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000378:metabolism; D036145:Ginsenosides / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D005894:Panax / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D036145:Ginsenosides; D012503:Saponins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor