title: ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
abstract: The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. We have found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with histone H2AZ in nucleosome arrays. Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery.
authors.affiliation: Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 6068, Bethesda, MD 20892-4255, USA.
authors.forename: Gaku
authors.initials: G
authors.lastname: Mizuguchi
authors.forename: Xuetong
authors.initials: X
authors.lastname: Shen
authors.forename: Joe
authors.initials: J
authors.lastname: Landry
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wu
authors.forename: Subhojit
authors.initials: S
authors.lastname: Sen
authors.forename: Carl
authors.initials: C
authors.lastname: Wu
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002384:Catalysis; D020134:Catalytic Domain; D002843:Chromatin / Q000378:metabolism*; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D005800:Genes, Fungal; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016615:Telomere / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C481261:Swr1 protein, S cerevisiae