Functional BvgAS virulence control system in Bordetella bronchiseptica is necessary for induction of Ca2+ transients in ciliated tracheal epithelial cells.
To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca(2+) signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca(2+) transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca(2+) signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.
Groathouse
Nathan A
NA
Department of Zoology and Physiology, University of Wyoming, Laramie, Wyoming 82701, USA.
Heinzen
Robert A
RA
Boitano
Scott
S
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001885:Bordetella Infections / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000472:pathogenicity* / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002923:Cilia; D004847:Epithelial Cells / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009924:Organ Culture Techniques; D011817:Rabbits; D015398:Signal Transduction; D014132:Trachea / Q000166:cytology / Q000382:microbiology*; D014157:Transcription Factors / Q000378:metabolism*; D014774:Virulence
D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis; D002118:Calcium