Purification, crystallization and preliminary X-ray analysis of a water-soluble chlorophyll protein from Brassica oleracea L. var. acephala (kale).
A water-soluble chlorophyll protein (WSCP) with a chlorophyll a:b ratio of 6:1 from Brassica oleracea L. var. acephala (kale) was purified and crystallized by the hanging-drop vapour-diffusion method using PEG 8000 and zinc acetate as precipitants. The crystal belongs to the hexagonal space group P6(4)22, with unit-cell parameters a = b = 162.2, c = 38.7 A. A native data set was collected to 2.80 A resolution at 293 K using Cu Kalpha radiation from a rotating-anode generator. Preliminary analysis via molecular replacement identified one kale WSCP monomer in the asymmetric unit. The crystal packing showed a tetrameric structure for kale WSCP, as suggested by previous biochemical studies of WSCPs from Brassicaceae plants.
Horigome
Daisuke
D
Department of Biomolecular Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Satoh
Hiroyuki
H
Uchida
Akira
A
D000595:Amino Acid Sequence; D001937:Brassica / Q000737:chemistry*; D002734:Chlorophyll / Q000031:analogs & derivatives*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D020836:Protein Structure, Quaternary; D012995:Solubility; D013053:Spectrophotometry / Q000379:methods; D014867:Water
D045342:Light-Harvesting Protein Complexes; D014867:Water; D002734:Chlorophyll