A stearoyl-CoA-specific Delta 9 fatty acid desaturase from the basidiomycete Lentinula edodes.
A gene encoding a Delta 9 fatty acid desaturase (Delta 9-desaturase) was isolated from L. edodes. The open reading frame of this gene (named Le-FAD1) consisted of 1416 bp. The predicted protein of 471 amino acids shares 47-57% sequence identity with Delta 9-desaturases from other fungi. Expression of the Le-FAD1 ORF complemented genetic disruption of the Saccharomyces cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis of the Le-FAD1-expressing S. cerevisiae ole1-disrupted strain showed that Le-FAD1 protein had slight activity with palmitic acid (16:0) as the substrate and high activity with stearic acid (18:0). Analysis of transcriptional expression showed that the levels of Le-FAD1 mRNA in the primordium and fruiting body of L. edodes were higher than in mycelia cultivated at 18 degrees C as well as 25 degrees C. These results correlated with the increase of unsaturated fatty acids (UFAs) in total lipids during fruiting-body formation.
Sakai
Hiromichi
H
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.
Kajiwara
Susumu
S
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D007700:Kinetics; D020074:Lentinula / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013230:Stearoyl-CoA Desaturase; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; D017931:DNA Primers; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; C017725:stearoyl-coenzyme A; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase