26S proteasome subunits are O-linked N-acetylglucosamine-modified in Drosophila melanogaster.
The O-linked glycosylation of highly purified Drosophila 26S proteasome has been analyzed by immunological and lectin-binding methods. Five regulatory complex subunits and at least nine catalytic core subunits were recognized by two different monoclonal antibodies specific for O-linked N-acetylglucosamine-modified proteins, and by wheat germ agglutinin, which is specific for the N-acetylglucosamine sugar side-chain. The specificity of these reactions has been proved by competition studies with free N-acetylglucosamine. Three ATPase subunits of the regulatory complex, which are O-glycosylated, have previously been shown [FEBS Lett. 430 (1998) 269] to occur in phosphorylated form as well, indicating that several different post-translational modifications, with distinct regulatory potential, may be present on the same subunit.
Sümegi
Máté
M
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary.
Hunyadi-Gulyás
Eva
E
Medzihradszky
Katalin F
KF
Udvardy
Andor
A
D000117:Acetylglucosamine / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001665:Binding Sites; D004331:Drosophila melanogaster / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010100:Oxygen / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D021122:Protein Subunits; D014909:Wheat Germ Agglutinins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D010100:Oxygen; D000117:Acetylglucosamine