Oxidative stress in hypertensive,diabetic, and diabetic hypertensive rats.
BACKGROUND
Reactive oxygen species play a key role in the formation of endothelial dysfunction accompanying diabetes mellitus, hypertension, and other cardiovascular diseases.


METHODS
This study compares oxidative stress (OS) in Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), non-insulin-dependent Cohen Diabetic rats (CDR), and Cohen Rosenthal diabetic hypertensive rats (CRDH), a unique animal model of both diabetes and hypertension. The OS was evaluated with a newly developed thermochemiluminiscence (TCL) analyzer (Lumitest Ltd., Nesher, Israel) that measures the oxidizability (ie, susceptibility to oxidation) of a test sample.


RESULTS
The TCL oxidizability test results of sera from the different rats groups showed a time-dependent increase in TCL of up to 145% +/- 7% for WKY, 160% +/- 8% for SHR, 179% +/- 12% for CDR, and 226% +/- 15% for CRDH. These results were significant: P <.001 for SHR and CDR and P <.0001 for CRDH in comparison to WKY. Lipid peroxide levels also increased in each strain of rats: to 80 +/- 7.8 nmol/mL in WKY, 104 +/- 10.1 nmol/mL in SHR, 110 +/- 9.4 nmol/mL in CDR, and 167 +/- 11.7 nmol/mL in CRDH. These results were also significant: P <.001 for SHR, CDR and CRDH in comparison to WKY.


CONCLUSIONS
The combination of hypertension and diabetes is accompanied by higher oxidative stress than that seen with either disorder alone.
Friedman
Joseph
J
A.J. Chorley Institute for Hypertension, Chaim Sheba Medical Center, Tel Aviv University, Sackler School of Medicine, Tel Hashomer, Israel.
Peleg
Edna
E
Kagan
Tamara
T
Shnizer
Sergei
S
Rosenthal
Talma
T
D000818:Animals; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000503:physiopathology*; D006973:Hypertension / Q000150:complications / Q000503:physiopathology*; D008054:Lipid Peroxides / Q000378:metabolism; D008163:Luminescent Measurements; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017382:Reactive Oxygen Species / Q000378:metabolism*
D008054:Lipid Peroxides; D017382:Reactive Oxygen Species