Capsule thickness and amounts of a 39 kDa capsular protein of avian Pasteurella multocida type A strains correlate with their pathogenicity for chickens.
Capsule thickness of avian Pasteurella multocida type A strains was determined by transmission electron microscopy after labeling with polycationic ferritin and compared with their pathogenicity for chickens. The capsule thickness of P. multocida strains Pm-18 and X-73 was 81.4 and 50.1 nm on average, respectively. These strains were highly virulent for chicken, whereas the less virulent strains Pm-1 and Pm-3 had a thin and irregular capsule, 21.0 and 29.8 nm on average, respectively. However, the thickest capsule was observed in strain P-1059, 101.2 nm on average, and the strain revealed moderate virulence. The noncapsulated variant P-1059B, which was derived from strain P-1059, revealed low virulence. The six P. multocida strains were examined with regard to protein content on the capsule of organisms. Amounts of total proteins of crude capsular extract (CCE) from capsulated strains were approximately twice those of the noncapsulated strains. The amount of an antigenic 39 kDa protein in the CCE were found to correlate with the capsule thickness, since heavily capsulated strains exhibited the greatest amount, whereas noncapsulated strains including noncapsulated and low virulent variant P-1059B possessed little 39 kDa protein. The results demonstrated that the capsule thickness and the quantity of a 39 kDa capsular protein of avian P. multocida type A strains correlated with their pathogenicity for chickens.
Borrathybay
Entomack
E
Department of Veterinary Microbiology, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180-8602, Japan.
Sawada
Takuo
T
Kataoka
Yasushi
Y
Okiyama
Emi
E
Kawamoto
Eiichi
E
Amao
Hiromi
H
D000818:Animals; D001422:Bacterial Adhesion; D016667:Bacterial Capsules / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D002645:Chickens; D015151:Immunoblotting; D008854:Microscopy, Electron / Q000662:veterinary; D008970:Molecular Weight; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D011201:Poultry Diseases / Q000382:microbiology*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013234:Stem Cells; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins