Approach to the study of C-glycosyl flavones by ion trap HPLC-PAD-ESI/MS/MS: application to seeds of quince (Cydonia oblonga).
Ion trap HPLC-PAD-ESI/MS/MS has been used to study C-glycosyl flavones in quince seeds. Comparative analysis of the ions [(M-H)-60]-, [(M-H)-90]- and [(M-H)-120]- from 6-C- and 8-C-glycosyl flavone isomers, together with their respective retention times, allowed deductions to be made about the nature of the sugar units and the positions of C-glycosylation. Vicenin-2 (6,8-di-C-glucosyl apigenin), lucenin-2 (6,8-di-C-glucosyl luteolin), stellarin-2 (6,8-di-C-glucosyl chrysoeriol), isoschaftoside (6-C-arabinosyl-8-C-glucosyl apigenin), schaftoside (6-C-glucosyl-8-C-arabinosyl apigenin), 6-C-pentosyl-8-C-glucosyl chrysoeriol and 6-C-glucosyl-8-C-pentosyl chrysoeriol were identified in quince seed.
Ferreres
Federico
F
Laboratorio de Fitoquímica, Departamento de Ciencia y Tecnología de los Alimentos, CEBAS (CSIC), PO Box 4195, E-30080 Murcia, Spain.
Silva
Branca M
BM
Andrade
Paula B
PB
Seabra
Rosa M
RM
Ferreira
Margarida A
MA
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D027824:Rosaceae / Q000737:chemistry*; D012639:Seeds; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D006027:Glycosides