Chromatographic determination of the association constant between 8-methoxypsoralen and modified beta-cyclodextrin: protective effect of hydroxypropyl-beta-cyclodextrin on 8-methoxypsoralen toxicity in human keratinocytes.
The retention of 8-methoxypsoralen (8-MOP) on an immobilised hydroxypropyl-beta-cyclodextrin (HP-beta-CD) column was analysed in HPLC by the determination of its Langmuir distribution isotherm. A such method was used to confirm the potential drug complexing role of this cyclodextrin. The 8-MOP/HP-beta-CD association constant (K) was equal to 29.5 and 18.7 M-1, respectively, at a temperature equal to 5 and 25 degrees C, respectively. These association constant values were used to determine the cytotoxicity profile of human keratinocyte cell line (HaCaT) in relation to the complex concentration. It was showed through these data that HP-beta-CD had a cytoprotective since a reverse effect of HP-beta-CD on 8-MOP cytotoxicity was observed.
Guillaume
Y
Y
Equipe des Sciences Séparatives et Biopharmaceutiques, Faculté de Médecine et de Pharmacie, Place Saint Jacques, 25030 Besançon, France. yves.guillaume@univ-fcomte.fr
Andre
C
C
Simon
N
N
Gehin
A
A
Guyon
C
C
Thomassin
M
M
Ismaili
L
L
Aubin
F
F
Nicod
L
L
D002460:Cell Line; D003505:Cyclodextrins / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D008730:Methoxsalen / Q000032:analysis* / Q000633:toxicity; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; D008730:Methoxsalen