Reduction of antigenicity of Cry j I, major allergen of Japanese cedar pollen, by the attachment of polysaccharides.
An attempt was made to mask the allergenic structure of a major allergen protein, Cry j I (CJI), in Japanese cedar pollen using the Maillard-type polysaccharide conjugation. The SDS-PAGE pattern of the CJI-galactomannan conjugate prepared by the Maillard reaction showed broad bands widely distributed from 50 kDa to more than 100 kDa, suggesting the attachment of galactomannan. The competitive enzyme-linked immunosorbent assay showed that the IgE antibody in the sera of cedar pollen-sensitive patients reacted strongly with CJI, while it did not react with the CJI-galactomannan conjugate. This result suggests that the antigenicity of CJI is greatly reduced by the conjugation with galactomannan.
Usui
Masakatsu
M
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Saito
Akira
A
Taniguchi
Naohiro
N
Nishijima
Noriaki
N
Azakami
Hiroyuki
H
Kato
Akio
A
D000485:Allergens / Q000187:drug effects / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D052179:Antigens, Plant; D028203:Cedrus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007564:Japan; D010940:Plant Proteins / Q000187:drug effects / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011134:Polysaccharides / Q000494:pharmacology*
D000485:Allergens; D000906:Antibodies; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010940:Plant Proteins; D011134:Polysaccharides; D007073:Immunoglobulin E