Use of a lectin as an enterocyte-specific cell surface marker for flow cytometric analysis of isolated native small intestinal epithelial cells.
Little use has been made of flow cytometry in evaluating small intestinal epithelial cells. Obtaining pure epithelial cell populations devoid of peripheral blood contaminants and intraepithelial lymphocytes contributes to the difficulties encountered in flow cytometry studies. We have investigated the use of lectins as enterocyte specific cell markers using lectin histochemistry, and have identified one lectin, UEA-1, which binds exclusively and specifically to intestinal epithelial cell brush border. Additionally, we have exploited that specificity using flow cytometry and FITC-UEA-1 to identify and separate native intestinal epithelial cells from a mixed cell population isolated by mechanical vibration. This fluorescent-lectin technique is a unique and simple method to identify native small intestinal epithelial cells in a mixed cell population; it may be exploited by flow cytometric sorting of a pure population for biochemical study or as an enterocyte specific label for surface receptor flow cytometric studies in the research or clinical setting.
Albers
T M
TM
Department of Pathology, Tufts University, School of Medicine and Veterinary Medicine, Boston, Massachusetts 02111, USA.
Moore
R P
RP
D000818:Animals; D015415:Biomarkers; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism
D015415:Biomarkers; D005456:Fluorescent Dyes; D037102:Lectins; D008565:Membrane Proteins