Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan.
Five strains of halophilic, Gram-negative marine bacteria (KMM 3809(T), KMM 3814, KMM 3815, KMM 3817 and KMM 3818) were isolated from sediments collected from Chazhma Bay, Sea of Japan. Phylogenetic 16S rRNA gene sequence-based analysis placed these bacteria in a clade within the genus Marinobacter in the gamma-Proteobacteria. KMM 3809(T) showed highest 16S rRNA gene sequence similarity of 97.3 % to Marinobacter litoralis and 96.9 % to Marinobacter hydrocarbonoclasticus and Marinobacter aquaeolei. DNA-DNA hybridization between the five isolates was at the conspecific level (94-96 %) and that among the closest phylogenetic neighbours ranged from 45.0 to 62.5 %. The new organisms were susceptible to polymyxin. Predominant fatty acids were C(16 : 0), C(16 : 1)omega9c, C(16 : 1)omega7c and C(18 : 1)omega9c. Phylogenetic evidence, along with phenotypic and genotypic characteristics, showed that the bacteria constituted a novel species of the genus Marinobacter. The name Marinobacter excellens sp. nov. is proposed for this species, with the type strain KMM 3809(T) (=CIP 107686(T)).
Gorshkova
Nataliya M
NM
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Ivanova
Elena P
EP
Sergeev
Alexandr F
AF
Zhukova
Natalia V
NV
Alexeeva
Yulia
Y
Wright
Jonathan P
JP
Nicolau
Dan V
DV
Mikhailov
Valery V
VV
Christen
Richard
R
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology; D007091:Image Processing, Computer-Assisted; D007564:Japan; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology
D004269:DNA, Bacterial