Cloning and characterization of a novel Hsp100/Clp gene (osClpD) from Oryza sativa.
A novel osClpD gene, encoding a highly conservative ClpD subfamily member, was first isolated and characterized from Oryza sativa. The full-length cDNA of osClpD gene was 3140 bp and contained a 2884 bp open reading frame encoding a 938 amino acid protein. The phylogenetic tree and blast search showed that OSClpD belonged to the ClpD subfamily of the Hsp100/Clp family, and contained all protein motifs characteristic for the ClpD subfamily of Hsp100/Clp proteins. The real-time quantitative PCR analysis proved that it was inducible by water deficit and temperature stress in vegetative tissues.
Shen
Guo-An
GA
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Pang
Yong-Zhen
YZ
Lin
Chang-Fa
CF
Wei
Chun
C
Qian
Xiao-Yin
XY
Jiang
Li-Zhi
LZ
Du
Xi-Ling
XL
Li
Ke-Gui
KG
Attia
Kotb
K
Yang
Jin-Shui
JS
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003890:Desiccation; D049071:Endopeptidase Clp; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics*; D013696:Temperature
D017931:DNA Primers; D018076:DNA, Complementary; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases