title: Genetic analysis of the capsule locus of Haemophilus influenzae serotype f.
abstract: A 19-kb DNA region containing genes involved in the biosynthesis of the capsule of Haemophilus influenzae serotype f (Hif) has been cloned and characterized. The Hif cap locus organization is typical of group II capsule biosynthetic loci found in other H. influenzae serotype b bacteria and other gram-negative bacteria. However, the Hif cap locus was not associated with an IS1016 element. Three new open reading frames, Fcs1, Fcs2, and Fcs3, were identified in the Hif capsule-specific region II. The chromosomal location of the Hif cap locus and the organization of the flanking sequences differed significantly from previously described division I H. influenzae serotypes.
authors.affiliation: Atlanta Veterans Affairs Medical Center and Department of Medicine, Emory University School of Medicine, Decatur, Georgia 30033, USA.
authors.forename: Sarah W
authors.initials: SW
authors.lastname: Satola
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Schirmer
authors.forename: Monica M
authors.initials: MM
authors.lastname: Farley
mesh_terms: D016667:Bacterial Capsules / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins