title: Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging.
abstract: Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.
authors.affiliation: Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, U.K.
authors.forename: I
authors.initials: I
authors.lastname: Karakikes
authors.forename: R E
authors.initials: RE
authors.lastname: Barber
authors.forename: I E G
authors.initials: IE
authors.lastname: Morrison
authors.forename: N
authors.initials: N
authors.lastname: Fernández
authors.forename: R J
authors.initials: RJ
authors.lastname: Cherry
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; C045084:invariant chain