[Characterization of a Cl. Perfringens type D strain, isolated in the field and optimization of epsilon toxin biosynthesis in a cell culture].
A field strain of cl. perfringens, named Dt001, was isolated from kidney of ovine enterotoemia case. The isolate characterized as Cl. perfringens, type D was based on its cultural and biochemical characters and its factors of virulence. The strain was very toxinogenic and well adapted to culture conditions of biofermentation when the parameters related to ptt, incubation time, substrat ... were optimized. Thus, the use of carbon source as polymer (destrine), the continuous control of pH allowed improvement of the rate of biosynthesis of Epsilon toxine by 10 times. The study of the immunogenicity of the isolate showed that preparations of anacultures were more immunogenic then those of anatoxine type. The fact that the two forms of epsilon antigens (protoxin and active toxin) show similar immune response in rabbits, indicates that the proteolytic action of trypsin is limited only to the toxic sites and does not affect the immunogenic epsitopes of the toxin. It also suggests a molecular organization of epsilon toxin in which the immunogenic epsitopes and the toxin sites are apart. The biotechnological performances and the immunogenicity and toxinogenical of the Dt001 isolate are in favor of its possible use as a component of an inactivated vaccine against enterotoxenia.
Maaroufi
A
A
Laboratoire de Microbiologie Vétérinaire, Institut Pasteur de Tunis.
Metoui
W
W
Rahmouni
S
S
Ghram
A
A
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods*; D003016:Clostridium perfringens / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D004767:Enterotoxemia / Q000453:epidemiology / Q000382:microbiology*; D007114:Immunization / Q000379:methods; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin; D016056:Immunodominant Epitopes