Transcription of metabolic enzyme genes during the excystation of Giardia lamblia.
The present study evaluates the expression of genes of Giardia lamblia, one of the most simple and most early diverging eukaryotes, that encode the metabolic enzymes pyruvate: ferredoxin oxidoreductase (PFOR), acetyl-CoA synthetase (ACS), alcohol dehydrogenase E (ADHE) and glutamate dehydrogenase (GDH) and the cyst wall protein (CWP1) gene in trophozoites, cysts and during the excystation process. Primers were designed to amplify mRNA fragments through quantitative reverse-transcriptase-polymerase-chain-reaction. In trophozoites, all transcripts of the enzymes studied were present. In cysts, three of the transcripts were detected: CWP1, GDH and ACS; but the relative levels of the mRNA of GDH and ACS were very different between trophozoites and cysts. During excystation, PFOR and ADHE transcripts appeared after the first induction phase, and the mRNAs of ACS and GDH increased throughout the process.
Niño
Carlos A
CA
Laboratorio de Investigaciones Básicas en Bioquímica, Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Bogota, Colombia.
Wasserman
Moises
M
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000818:Animals; D003066:Coenzyme A Ligases / Q000235:genetics; D017931:DNA Primers; D005287:Ferredoxin-NADP Reductase / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D016829:Giardia lamblia / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D005969:Glutamate Dehydrogenase / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
C094333:CWP1 protein, Giardia lamblia; D017931:DNA Primers; D015800:Protozoan Proteins; D012333:RNA, Messenger; D000426:Alcohol Dehydrogenase; D005287:Ferredoxin-NADP Reductase; D005969:Glutamate Dehydrogenase; D003066:Coenzyme A Ligases; C101958:acetate-CoA ligase (ADP-forming)