title: Fuzzy cluster analysis of high-field functional MRI data.
abstract: Functional magnetic resonance imaging (fMRI) based on blood-oxygen level dependent (BOLD) contrast today is an established brain research method and quickly gains acceptance for complementary clinical diagnosis. However, neither the basic mechanisms like coupling between neuronal activation and haemodynamic response are known exactly, nor can the various artifacts be predicted or controlled. Thus, modeling functional signal changes is non-trivial and exploratory data analysis (EDA) may be rather useful. In particular, identification and separation of artifacts as well as quantification of expected, i.e. stimulus correlated, and novel information on brain activity is important for both, new insights in neuroscience and future developments in functional MRI of the human brain. After an introduction on fuzzy clustering and very high-field fMRI we present several examples where fuzzy cluster analysis (FCA) of fMRI time series helps to identify and locally separate various artifacts. We also present and discuss applications and limitations of fuzzy cluster analysis in very high-field functional MRI: differentiate temporal patterns in MRI using (a) a test object with static and dynamic parts, (b) artifacts due to gross head motion artifacts. Using a synthetic fMRI data set we quantitatively examine the influences of relevant FCA parameters on clustering results in terms of receiver-operator characteristics (ROC) and compare them with a commonly used model-based correlation analysis (CA) approach. The application of FCA in analyzing in vivo fMRI data is shown for (a) a motor paradigm, (b) data from multi-echo imaging, and (c) a fMRI study using mental rotation of three-dimensional cubes. We found that differentiation of true "neural" from false "vascular" activation is possible based on echo time dependence and specific activation levels, as well as based on their signal time-course. Exploratory data analysis methods in general and fuzzy cluster analysis in particular may help to identify artifacts and add novel and unexpected information valuable for interpretation, classification and characterization of functional MRI data which can be used to design new data acquisition schemes, stimulus presentations, neuro(physio)logical paradigms, as well as to improve quantitative biophysical models.
authors.affiliation: NMR Group, Institute for Medical Physics, University of Vienna, Währingerstrasse 13, A-1090 Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Windischberger
authors.forename: Markus
authors.initials: M
authors.lastname: Barth
authors.forename: Claus
authors.initials: C
authors.lastname: Lamm
authors.forename: Lee
authors.initials: L
authors.lastname: Schroeder
authors.forename: Herbert
authors.initials: H
authors.lastname: Bauer
authors.forename: Ruben C
authors.initials: RC
authors.lastname: Gur
authors.forename: Ewald
authors.initials: E
authors.lastname: Moser
mesh_terms: D016477:Artifacts; D001931:Brain Mapping; D002560:Cerebrovascular Circulation; D016000:Cluster Analysis; D005385:Fingers / Q000502:physiology; D017143:Fuzzy Logic; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008606:Mental Processes / Q000502:physiology; D008962:Models, Theoretical; D009038:Motion; D009044:Motor Cortex / Q000000981:diagnostic imaging / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D010775:Photic Stimulation; D012372:ROC Curve; D013997:Time Factors; D014463:Ultrasonography
chemical_list: D010100:Oxygen