Interleukin-2 abolishes myeloid cell accumulation induced by Lewis lung carcinoma.
Immune aberration in cancer patients can be at least partly ascribed to an accumulation of immature myeloid cells and monocytes/macrophages with immunosuppressive functions. Mice implanted with Lewis lung carcinoma 2 (LL/2) cells show marked splenomegaly as the tumors progress, and this condition is accompanied by impaired T cell activities. We characterized the cells that accumulated in the spleens of LL/2 tumor-bearing mice and attempted to restore the normal cell population by employing interleukin-2 (IL-2). Flow cytometric analysis revealed that the cells expressing Mac1, B7, NK-K1, Gra-1, and MHC class II antigens on their surfaces drastically decreased in number when LL/2 had been engineered to produce IL-2. IL-2 also restored the concanavalin A (ConA)-mediated proliferative response and IL-2 production of the spleen cells. The in vivo growth of IL-2-producing tumors was significantly slower than that of parental LL/2 cells. Therefore, local IL-2 production may reverse systemic immune abnormality by stopping myeloid cell accumulation.
Shin-Ya
Masaharu
M
Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kitaku, Kyoto 603-8555, Japan. masaharu@basic.kpu-m.ac.jp
Mazda
Osam
O
Tsuchihara
Chiharu
C
Hirai
Hideyo
H
Imanishi
Jiro
J
Takeuchi
Minoru
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018827:Carcinoma, Lewis Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology*; D009368:Neoplasm Transplantation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D042461:Vascular Endothelial Growth Factor A; D003208:Concanavalin A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor