title: Regeneration of the vocal fold using autologous mesenchymal stem cells.
abstract: The aim of this study was to regenerate the injured vocal fold by means of selective cultured autologous mesenchymal stem cells (MSCs). Eight adult beagle dogs were used for this experiment. Selective incubation of MSCs from bone marrow was done. These MSCs were submitted to 3-dimensional incubation in 1% hydrochloric acid atelocollagen. Three-dimensional incubated MSCs were injected into the left vocal fold, and atelocollagen only was injected into the right vocal fold of the same dog as a control. Four days after injection, the posterior parts of the vocal folds were incised. The regeneration of the vocal fold was estimated by morphological and histologic evaluations. Our results showed that 3-dimensional incubated MSCs were useful in the regeneration of the injured vocal fold. This study shows that damaged tissues such as an injured vocal fold would be able to be regenerated by tissue engineering.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kyoto University Faculty of Medicine, Kyoto, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kanemaru
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Kojima
authors.forename: Akhmar
authors.initials: A
authors.lastname: Magrufov
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hiratsuka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D012038:Regeneration; D023822:Tissue Engineering; D014182:Transplantation, Autologous; D014827:Vocal Cords / Q000293:injuries* / Q000502:physiology