Hydrogen peroxide-induced apoptosis in pc12 cells and the protective effect of puerarin.
In this study, the effect of puerarin on hydrogen peroxide-induced apoptosis in PC12 cells was studied. Exposure of cells to 0.5mM H(2)O(2)may cause significant viability loss and apoptotic rate increase. When c-Myc, Bcl-2 and Bax expression and caspase-3 activity were measured, using Ac-DEVD-AMC as a substrate, the changes in these apoptosis regulatory and effector proteins suggested that the elevation of c-Myc, decrease in Bcl-2:Bax protein ratio, and caspase-3 activation all play a key role in apoptosis. When cells were treated with puerarin prior to 0.5 mM H(2)O(2)treatment, a reduction in viability loss and apoptotic rate was seen. In addition, c-Myc expression decreased and Bcl-2:Bax ratio increased. Puerarin also reduced the H(2)O(2)-induced elevation of caspase-3 activation. These results suggest that puerarin can protect neurons against oxidative stress. It can block apoptosis in its early stages via the regulation of anti- and pro-apoptotic proteins, as well as by the attenuation of caspase-3 activation in H(2)O(2)-induced PC12 cells.
Jiang
B
B
Department of Biochemical Engineering, Dalian University of Technology, 116024, China.
Liu
J H
JH
Bao
Y M
YM
An
L J
LJ
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival; D003374:Coumarins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D008956:Models, Chemical; D009842:Oligopeptides / Q000494:pharmacology; D018384:Oxidative Stress; D016716:PC12 Cells; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology; D051028:bcl-2-Associated X Protein
C112287:Ac-aspartyl-glutamyl-valyl-aspartyl-aminomethylcoumarin; C494828:Bax protein, rat; D003374:Coumarins; D007529:Isoflavones; D009842:Oligopeptides; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D014665:Vasodilator Agents; D051028:bcl-2-Associated X Protein; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; C033607:puerarin