title: Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS.
abstract: In the present study, we describe the human and mouse RFP2 gene structure, multiple RFP2 mRNA isoforms in the two species that have different 5' UTRs and a human-specific antisense transcript RFP2OS. Since the human RFP2 5' UTR is not conserved in mouse, these findings might indicate a different regulation of RFP2 in the two species. The predicted human and mouse RFP2 proteins are shown to contain a tripartite RING finger-B-box-coiled-coil domain (RBCC), also known as a TRIM domain, and therefore belong to a subgroup of RING finger proteins that are often involved in developmental and tumorigenic processes. Because homozygous deletions of chromosomal region 13q14.3 are found in a number of malignancies, including chronic lymphocytic leukemia (CLL) and multiple myeloma (MM), we suggest that RFP2 might be involved in tumor development. This study provides necessary information for evaluation of the role of RFP2 in malignant transformation and other biological processes.
authors.affiliation: Genome Analysis Laboratory, Institute of General Genetics, Russian Academy of Science, Moscow 119991, Russia. abaranov@gmu.edu
authors.forename: Ancha
authors.initials: A
authors.lastname: Baranova
authors.forename: Marianne
authors.initials: M
authors.lastname: Hammarsund
authors.forename: Dmitry
authors.initials: D
authors.lastname: Ivanov
authors.forename: Mikhail
authors.initials: M
authors.lastname: Skoblov
authors.forename: Olle
authors.initials: O
authors.lastname: Sangfelt
authors.forename: Martin
authors.initials: M
authors.lastname: Corcoran
authors.forename: Tatiana
authors.initials: T
authors.lastname: Borodina
authors.forename: Natalia
authors.initials: N
authors.lastname: Makeeva
authors.forename: Anna
authors.initials: A
authors.lastname: Pestova
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tyazhelova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Nazarenko
authors.forename: Francesco
authors.initials: F
authors.lastname: Gorreta
authors.forename: Tariq
authors.initials: T
authors.lastname: Alsheddi
authors.forename: Karen
authors.initials: K
authors.lastname: Schlauch
authors.forename: Eugene
authors.initials: E
authors.lastname: Nikitin
authors.forename: Bagrat
authors.initials: B
authors.lastname: Kapanadze
authors.forename: Dmitry
authors.initials: D
authors.lastname: Shagin
authors.forename: Andrey
authors.initials: A
authors.lastname: Poltaraus
authors.forename: Andrey
authors.initials: A
authors.lastname: Ivanovich Vorobiev
authors.forename: Eugene
authors.initials: E
authors.lastname: Zabarovsky
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukianov
authors.forename: Vikas
authors.initials: V
authors.lastname: Chandhoke
authors.forename: Rachel
authors.initials: R
authors.lastname: Ibbotson
authors.forename: David
authors.initials: D
authors.lastname: Oscier
authors.forename: Stefan
authors.initials: S
authors.lastname: Einhorn
authors.forename: Dan
authors.initials: D
authors.lastname: Grander
authors.forename: Nick
authors.initials: N
authors.lastname: Yankovsky
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005260:Female; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; C112217:TRIM13 protein, human; D025521:Tumor Suppressor Proteins; D004247:DNA