title: Polychromatic LED therapy in burn healing of non-diabetic and diabetic rats.
abstract: OBJECTIVE
  We determined the effect of polychromatic light-emitting diodes (LED) in burn healing of non-diabetic and streptozotocin-induced diabetic rats.
  
  
  BACKGROUND
  LEDs were used as the light source for phototherapy.
  
  
  METHODS
  The polychromatic LED is a cluster of 25 diodes emitting photons at wavelengths of 510-543, 594-599, 626-639, 640-670, and 842-879 nm with 272-mW output power. Age-matched, male Sprague-Dawley rats (n = 30) were used. Streptozotocin (70 mg/kg) was used for diabetes induction. Rat weight, hyperglycemia, and glycosuria were monitored for the first 3 days and weekly thereafter. Rats were anesthetized and shaved after 1 week of diabetes. Burn areas of 1.5 +/-.03 cm2 were created using a metal rod pre-heated up to 600 degrees C that was applied for 2 sec. Diabetic and non-diabetic rats were randomized into the following treatment groups: control, 5, 10, 20, and 30 J/cm2. Light treatment commenced after burn infliction and was repeated three times per week. Burn areas were measured daily.
  
  
  RESULTS
  Burn healing was impaired significantly during diabetes by -46.17%. Polychromatic LED treatment using 5, 10, 20, and 30 J/cm2 incident doses influenced healing by 6.85%, 4.93%, -4.18%, and -5.42% in the non-diabetic rats; and 73.87%, 76.77%, 60.92%, and 48.77% in the diabetic rats, relative to their controls, respectively.
  
  
  CONCLUSIONS
  The effect of polychromatic LED in non-diabetic rats was insignificant; however, it simulated the trend of stimulation and inhibition seen using low-level lasers. Significant stimulation observed in the diabetic rats demonstrated the usefulness of polychromatic LED in diabetic burn healing.
authors.affiliation: Laser Medicine Research Section, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. Watban@KFSHRC.EDU.SA
authors.forename: Farouk A H
authors.initials: FA
authors.lastname: Al-Watban
authors.forename: Bernard L
authors.initials: BL
authors.lastname: Andres
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications / Q000532:radiotherapy / Q000628:therapy*; D048909:Diabetes Complications; D003921:Diabetes Mellitus, Experimental; D008297:Male; D023421:Models, Animal; D010789:Phototherapy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000528:radiation effects*