title: Anaphylaxis from topical bovine thrombin (Thrombostat) during haemodialysis and evaluation of sensitization among a dialysis population.
abstract: BACKGROUND
  Topical bovine thrombin (TBT) is used as a topical haemostatic agent in haemodialysis (HD) units and for surgical procedures. It has caused allergic reactions during surgical procedures, but no previous cases have been reported with dialysis usage.
  
  
  OBJECTIVE
  A case is reported of anaphylaxis due to TBT in an HD patient. This led to a determination of whether HD patients exposed to TBT develop specific IgE and IgG antibodies to TBT and an assessment of the frequency of allergic symptoms in HD patients as compared with peritoneal dialysis (PD) patients.
  
  
  METHODS
  A cross-sectional study of 100 HD and 52 PD patients was performed using a questionnaire to determine allergic symptoms. An ELISA assay was developed to determine TBT-specific IgE and IgG serum antibodies among HD patients. HD patients without TBT exposure and age- and gender-matched blood donors served as controls.
  
  
  RESULTS
  Allergic symptoms temporally related to dialysis reported by a questionnaire were significantly more frequent for HD patients than for PD patients: rhinoconjuctivitis 11% vs. 2%, asthma symptoms 20% vs. 6%, urticaria 23% vs. 8% (P values <0.05). Positive TBT-specific IgE and IgG antibodies were more frequent among 65 exposed HD patients (28% for IgE and 26% for IgG) than for controls (4% and 9%), P<0.05.
  
  
  CONCLUSIONS
  Results indicate the potential for sensitization and clinical allergic responses to beef thrombin when used for haemostasis in HD and suggest that other haemostatic methods should be considered.
authors.affiliation: University Health Network, Toronto, Ontario, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Wai
authors.forename: V
authors.initials: V
authors.lastname: Tsui
authors.forename: Z
authors.initials: Z
authors.lastname: Peng
authors.forename: R
authors.initials: R
authors.lastname: Richardson
authors.forename: D
authors.initials: D
authors.lastname: Oreopoulos
authors.forename: S M
authors.initials: SM
authors.lastname: Tarlo
mesh_terms: D000328:Adult; D000368:Aged; D000707:Anaphylaxis / Q000139:chemically induced* / Q000276:immunology; D000906:Antibodies / Q000097:blood; D016009:Chi-Square Distribution; D005260:Female; D006490:Hemostatics / Q000009:adverse effects*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D010530:Peritoneal Dialysis; D006435:Renal Dialysis; D013917:Thrombin / Q000009:adverse effects*
chemical_list: D000906:Antibodies; D006490:Hemostatics; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D013917:Thrombin