title: Intracellular domains of NMDA receptor subtypes are determinants for long-term potentiation induction.
abstract: NMDA receptors (NMDARs) are essential for modulating synaptic strength at central synapses. At hippocampal CA3-to-CA1 synapses of adult mice, different NMDAR subtypes with distinct functionality assemble from NR1 with NR2A and/or NR2B subunits. Here we investigated the role of these NMDA receptor subtypes in long-term potentiation (LTP) induction. Because of the higher NR2B contribution in the young hippocampus, LTP of extracellular field potentials could be enhanced by repeated tetanic stimulation in young but not in adult mice. Similarly, NR2B-specific antagonists reduced LTP in young but only marginally in adult wild-type mice, further demonstrating that in mature CA3-to-CA1 connections LTP induction results primarily from NR2A-type signaling. This finding is also supported by gene-targeted mutant mice expressing C-terminally truncated NR2A subunits, which participate in synaptic NMDAR channel formation and Ca2+ signaling, as indicated by immunopurified synaptic receptors, postembedding immunogold labeling, and spinous Ca2+ transients in the presence of NR2B blockers. These blockers abolished LTP in the mutant at all ages, revealing that, without the intracellular C-terminal domain, NR2A-type receptors are deficient in LTP signaling. Without NR2B blockade, CA3-to-CA1 LTP was more strongly reduced in adult than young mutant mice but could be restored to wild-type levels by repeated tetanic stimulation. Thus, besides NMDA receptor-mediated Ca2+ influx, subtype-specific signaling is critical for LTP induction, with the intracellular C-terminal domain of the NR2 subunits directing signaling pathways with an age-dependent preference.
authors.affiliation: Max-Planck-Institute for Medical Research, D-69120 Heidelberg, Germany. kohr@mpimf-heidelberg.mpg.de
authors.forename: Georg
authors.initials: G
authors.lastname: Köhr
authors.forename: Vidar
authors.initials: V
authors.lastname: Jensen
authors.forename: Helmut J
authors.initials: HJ
authors.lastname: Koester
authors.forename: Andre L A
authors.initials: AL
authors.lastname: Mihaljevic
authors.forename: Jo K
authors.initials: JK
authors.lastname: Utvik
authors.forename: Ane
authors.initials: A
authors.lastname: Kvello
authors.forename: Ole P
authors.initials: OP
authors.lastname: Ottersen
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeburg
authors.forename: Rolf
authors.initials: R
authors.lastname: Sprengel
authors.forename: Øivind
authors.initials: Ø
authors.lastname: Hvalby
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018390:Gene Targeting; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D017434:Protein Structure, Tertiary / Q000502:physiology; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D018691:Excitatory Amino Acid Antagonists; C409466:NR1 NMDA receptor; C120997:NR2A NMDA receptor; C121001:NR2B NMDA receptor; D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate