Expression of connexin48.5, connexin44.1, and connexin43 during zebrafish (Danio rerio) lens development.
Connexins (Cx), the protein units of gap junctions, play important roles in lens development and homeostasis. Here, we report the mRNA expression patterns of zebrafish Cx48.5, Cx44.1, Cx43 during lens development. The expression of all three connexins in the adult lens was first confirmed by reverse transcriptase-polymerase chain reaction. By whole-mount in situ hybridization, we detected Cx48.5 expression throughout the lens, except the lateral lens epithelium, at 36 hours postfertilization (hpf). The pattern remained the same at 2 days postfertilization (dpf). By 3 and 4 dpf, Cx48.5 expression was restricted to the differentiating lens fibers in the equatorial and medial regions. Cx44.1 was expressed in a similar manner as Cx48.5 from 36 hpf to 4 dpf. However, Cx44.1 expression was also detected in the lens at 24 hpf. Cx43 expression was detected throughout the lens at 24 and 36 hpf but became restricted to the lateral epithelium at later stages.
Cheng
Shaohong
S
Department of Zoology, University of Manitoba, Winnipeg, Manitoba, Canada.
Christie
Tara
T
Valdimarsson
Gunnar
G
D000818:Animals; D002645:Chickens; D018031:Connexin 43 / Q000096:biosynthesis*; D017630:Connexins / Q000096:biosynthesis*; D017931:DNA Primers / Q000494:pharmacology; D017629:Gap Junctions; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007908:Lens, Crystalline / Q000378:metabolism*; D051379:Mice; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014018:Tissue Distribution; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis*
D018031:Connexin 43; D017630:Connexins; D017931:DNA Primers; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C434318:connexin 44; C488372:connexin 48.5, zebrafish