Selenium toxicity in sheep grazing reclaimed phosphate mining sites.
Phosphate mining operations in southeastern Idaho have exposed selenium (Se) that was originally sequestered in the subsurface. Sheep grazing in these areas have died as a result of high Se concentrations in forage and water. This study was designed to monitor the health status of sheep grazing in a natural environment with known elevated levels of Se. A total of 72 Columbia x Suffolk sheep were divided into 3 treatment groups that included control (Con), low selenium (LoSe) and high selenium (HiSe). The baseline phase of the study was conducted in an area with normal background Se levels in forage and water, and was grazed for 3 w by all sheep groups. The sheep then were moved onto reclaimed mine areas to begin the 4-w exposure phase. This was followed by a 2-w depuration phase where sheep again received normal Se levels in forage and water. The Con group was held on areas with normal Se levels of forage (< 0.32 ppm Se dw) and water (< 1.70 ppb Se). The LoSe group was held in an area of elevated forage Se (< 13.0 ppm Se dw) and normal Se levels in their water (< 1.70 ppb Se) during the exposure phase. The HiSe group was held on mining areas with elevated Se forage (< 49.0 ppm Se dw) and drinking water (340 to 415 ppb Se). Whole blood and serum levels in the HiSe group peaked at 1.32 and 0.99 ppm mean Se, respectively. The LoSe group had mean whole blood and serum Se levels of 0.75 ppm on day 42 and 0.32 ppm on day 35 respectively. The Con group maintained low Se levels in both whole blood and serum that ranged from 0.05 to 0.14 ppm and 0.06 to 0.13 ppm respectively. The Se exposure in the HiSe group was estimated 0.26 mg Se/kg body weight/d. One sheep in the HiSe group died and was diagnosed with Se toxicosis based on clinical signs, histopathology and tissue Se levels. Se in liver (3.90 ppm), kidney (1.90 ppm) and skeletal muscle (0.70 ppm) were indicative of high to toxic Se exposure. Two other sheep necropsied after the exposure phase also had Se concentrations in liver, kidney and skeletal muscle representative of high or toxic Se exposure (5.50, 3.50 and 1.10 ppm Se), but these sheep had no gross or histopathological signs of illness. More research is needed on the toxicology of Se in sheep grazing natural settings.
Fessler
A J
AJ
University of Idaho, Department of Food Science and Toxicology, University of Idaho, Moscow, ID 83844-2201, USA.
Moller
G
G
Talcott
P A
PA
Exon
J H
JH
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000822:Animal Husbandry; D000818:Animals; D000831:Animals, Newborn; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000662:veterinary*; D004032:Diet; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D007061:Idaho; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008906:Mining; D018482:Muscle, Skeletal / Q000378:metabolism; D012643:Selenium / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D012709:Serum Albumin; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood* / Q000139:chemically induced
D004785:Environmental Pollutants; D012709:Serum Albumin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D012643:Selenium