title: Effects of leptin and leptin fragments on steroid secretion and proliferative activity of regenerating rat adrenal cortex.
abstract: Leptin, an adipose tissue-secreted hormone, acts via several isoforms of specific receptors (Ob-Rs), which may variously interact with the native leptin molecule and its fragments. Evidence has been provided that leptin affects rat adrenal functions, but the results were rather conflicting depending on the experimental condition examined (e.g. regenerating vs. mature or immature adrenal gland). Hence, we investigated the effects of three subcutaneous injections of murine leptin(1-147) and several leptin fragments (3 nmol/100 g body weight; 28, 16 and 4 h before the sacrifice) on the secretory activity and growth of regenerating rat adrenal cortex. The following leptin fragments were tested: murine leptin(116-130), and human leptin fragments 150-167, 138-167, 93-105, 22-56 and [Tyr]26-39. Leptin(1-147) enhanced plasma concentration of both aldosterone and corticosterone. The blood level of aldosterone was raised by leptin(116-130), leptin(138-167) and leptin(93-105), and that of corticosterone by leptin(93-105) and Tyr-leptin(26-39). Metaphase index (stachmokinetic method with vincristine) was unaffected by leptin(1-147), and lowered by leptin(116-130), leptin(150-167) and leptin(138-167). Collectively, our findings allow us to conclude that leptin and leptin fragments enhance the secretory activity and inhibit the growth of regenerating rat adrenal cortex, the biological activity of leptin being located in the C-terminal segment of its molecule.
authors.affiliation: Department of Histology and Embryology, Poznan School of Medicine, PL-60781 Poznan, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Markowska
authors.forename: Giuliano
authors.initials: G
authors.lastname: Neri
authors.forename: Anna
authors.initials: A
authors.lastname: Hochol
authors.forename: Magdalena
authors.initials: M
authors.lastname: Nowak
authors.forename: Gastone G
authors.initials: GG
authors.lastname: Nussdorfer
authors.forename: Ludwik K
authors.initials: LK
authors.lastname: Malendowicz
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects*; D000450:Aldosterone / Q000097:blood; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D003345:Corticosterone / Q000097:blood; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats
chemical_list: D020738:Leptin; D010446:Peptide Fragments; D000450:Aldosterone; D003345:Corticosterone