Role of Tea1p, Tea3p and Pom1p in the determination of cell ends in Schizosaccharomyces pombe.
Schizosaccharomyces pombe cells are rod-shaped and grow along a single axis from their two ends. Microtubules extend from the cell centre terminating at the cell ends. The ERM(ezrin/radixin/moesin)-like proteins Tea1p and Tea3p, and the Dyrk-like kinase Pom1p are cell end markers involved in the regulation of growth and microtubular dynamics at the cell ends. We have analysed the relative contribution of these three proteins to the determination of cell ends as sites both for cell growth and for microtubular termination. Pom1Delta, in combination with Tea1Delta or Tea3Delta, has the greatest difficulty in relocalizing actin to the cell ends following actin depolymerization and generates the most defective growth pattern. Tea1Delta, in combination with Pom1Delta or Tea3Delta, displays the highest number of microtubules bending round the cell ends. Tea1DeltaPom1Delta, which has the most defective growth pattern and microtubules, also displays the highest number of branched cells. We show that Tea1p, Tea3p and Pom1p all contribute, to different extents, to the determination of cell ends, as sites for both cell growth and microtubular termination. We also show that the fission yeast cell relies on both the positioning of landmarks and a properly organized microtubule cytoskeleton to direct cell growth.
Niccoli
Teresa
T
Cancer Research UK London Research Institute, Cell Cycle Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
Arellano
Manuel
M
Nurse
Paul
P
D000199:Actins / Q000037:antagonists & inhibitors / Q000502:physiology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology; D011494:Protein Kinases / Q000502:physiology*; D012568:Schizosaccharomyces / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014118:Toxins, Biological / Q000494:pharmacology
D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins; C432671:Tea1 protein, S pombe; C467709:Tea3 protein, S pombe; D013844:Thiazoles; D053778:Thiazolidines; D014118:Toxins, Biological; D011494:Protein Kinases; C112212:Pom1 protein, S pombe; C037067:latrunculin A