title: Comparison of enzyme activities in plasma and leukocytes in dairy and beef cattle.
abstract: Concentrations of plasma glucose, immunoreactive insulin (IRI) and free fatty acid (FFA) and activities of enzymes related to energy metabolism and lactate dehydrogenase (LDH) isoenzyme pattern in plasma and leukocytes were investigated in lactating Holstein cows (dairy cattle) and fattening Japanese Black Wagyu x Holstein steers (beef cattle). IRI concentrations and LDH and malate dehydrogenase (MDH) activities in the plasma of beef cattle were significantly higher than those in dairy cattle. The cytosolic ratio of MDH/LDH activity in the leukocytes of beef cattle was significantly higher than that of dairy cattle. These findings might be associated with the different energy metabolism between dairy and beef cattle.
authors.affiliation: Division of Veterinary Biochemistry, Department of Animal Science, Nippon Veterinary and Animal Science University, Masashino, Tokyo, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Akira
authors.initials: A
authors.lastname: Inoue
authors.forename: Akira
authors.initials: A
authors.lastname: Takeguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Megumi
authors.initials: M
authors.lastname: Shimoo
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
authors.forename: Itaru
authors.initials: I
authors.lastname: Yoshimura
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001786:Blood Glucose; D002417:Cattle / Q000097:blood* / Q000378:metabolism; D004586:Electrophoresis; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007328:Insulin / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology*
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007770:L-Lactate Dehydrogenase