title: Determination of the lipid classes and fatty acid profile of Niger (Guizotia abyssinica Cass.) seed oil.
abstract: Niger seeds (Guizotia abyssinica Cass.), which are of interest as a new source of vegetable oils, were subjected to Soxhlet-extraction with n-hexane and the extract analysed using a combination of CC, GC, TLC and normal-phase HPLC. The total lipid content was ca. 300 mg/g seed material, and the fatty acid profile showed a high content of linoleic acid (up to 63%) together with palmitic acid (17%), oleic acid (ca. 11%), and stearic acid (ca. 7%). CC separation over silica gel eluted with solvents of increasing polarity yielded 291 mg/g of neutral lipids, 5.76 mg/g of glycolipids, and 0.84 mg/g of phospholipids. GC analysis showed that the major fatty acid present in all lipid classes was linoleic acid together with minor amounts of palmitic, oleic and stearic acids. Polar lipid fractions, however, were characterised by higher levels of palmitic acid and a lower content of linoleic acid. Phospholipid classes separated by normal-phase HPLC consisted of phosphatidylcholine (ca. 49%), phosphatidylethanolamine (22%), phosphatidylinositol (14%), phosphatidylserine (ca. 8%), and minor amounts (2-3%) of phosphatidylglycerol and lysophosphatidylcholine.
authors.affiliation: Institute of Food Chemistry, Technical University of Berlin, TIB 4/3-1, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany. hassanienmohamed@hotmail.com
authors.forename: Mohamed Fawzy
authors.initials: MF
authors.lastname: Ramadan
authors.forename: Jörg-Thomas
authors.initials: JT
authors.lastname: Mörsel
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D010938:Plant Oils