Evolutionary models of the emergence of methicillin-resistant Staphylococcus aureus.
Five major lineages of methicillin-resistant Staphylococcus aureus (MRSA) have evolved since the introduction of methicillin for the treatment of infections caused by penicillin-resistant S. aureus in 1959. The clones of these lineages are responsible for the vast majority of hospital-acquired MRSA disease globally. We have constructed high-resolution evolutionary models for each lineage using a parsimony approach with 15 partial gene sequences from 147 geographically diverse isolates. On the basis of these models, we infer that MRSA has emerged at least 20 times upon acquisition of the methicillin resistance determinant, which is carried on a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec). The acquisition of SCCmec by sensitive clones was four times more common than the replacement of one SCCmec with another. Notably, SCCmec type IV was found in twice as many clones as any other SCCmec type, and it is this SCCmec type which is commonly found in clones from patients with community-acquired MRSA disease. Our findings suggest that most clones of MRSA arise by the acquisition of SCCmec type IV by methicillin-sensitive isolates.
Robinson
D Ashley
DA
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.
Enright
Mark C
MC
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers; D016106:Methicillin Resistance / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D009626:Terminology as Topic
D017931:DNA Primers