Application of L-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins.
Nanometer-sized L-cysteine-capped ZnS particles have been synthesized and used as a fluorescence probe to investigate the effect of proteins on fluorescent intensity. With Delta lambda=190 nm, maximum and constant synchronous fluorescence enhancement was produced at 267 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins has been developed. Under optimum conditions, calibration graphs are linear over the range 0.03-8.0 microg mL(-1) for bovine serum albumin (BSA), 0.01-6.0 microg mL(-1) for human serum albumin (HSA), 0.05-8.0 microg mL(-1) for gamma-globulin (gamma-G), and 0.04-4.0 microg mL(-1) for ovalbumin, respectively. The relative standard deviations of seven replicate measurements were 1.75% for 1.0 microg mL(-1) BSA, 1.90% for 1.0 microg mL(-1) HSA, 1.65% for 1.0 microg mL(-1) gamma-G, and 2.32% for 1.0 microg mL(-1) ovalbumin.
Zhu
Chang Qing
CQ
College of Chemistry and Materials Science, Anhui Normal University, 241000 Wuhu, P.R. China. Zhucq625@163.com
Zhao
Dan Hua
DH
Chen
Jin Long
JL
Li
Yong Xin
YX
Wang
Le Yu
le Y
Wang
Lun
L
Zhou
Yun You
YY
Zhuo
Shu Juan
SJ
Wu
Yu Qin
YQ
D002138:Calibration; D003545:Cysteine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008854:Microscopy, Electron; D036103:Nanotechnology; D011506:Proteins / Q000032:analysis*; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D005456:Fluorescent Dyes; D011506:Proteins; D013440:Sulfides; D017967:Zinc Compounds; D003545:Cysteine; C031238:zinc sulfide