[Expression of connective tissue growth factor gene in the hypertrophic scar and keloid tissue].
OBJECTIVE
To explore the effect of connective tissue growth factor on the pathogenesis of hypertrophic scar and keloid tissue.


METHODS
The content of hydroxyproline was determined and the expression of connective tissue growth factor gene was detected by the reverse transcription-polymerase chain reaction and image analysis technique in 5 normal skins, 15 hypertrophic scars and 7 keloid tissues.


RESULTS
The contents of hydroxyproline in the hypertrophic scar (84.10 +/- 1.76) and keloid tissue (92.38 +/- 2.04) were significantly higher than that of normal skin tissue (26.52 +/- 4.10) (P < 0.01). The index of connective tissue growth factor mRNA in the hypertrophic scar (0.78 +/- 0.63) and keloid tissue (0.84 +/- 0.04) were higher than that of normal skin tissue (0.09 +/- 0.25) (P < 0.01).


CONCLUSIONS
Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar and keloid tissue.
Liu
Jian-yi
JY
Department of Plastic and Aesthetic Surgery, Southwest Hospital of Third Military Medical University, Chongqing, P. R. China 400038.
Li
Shi-rong
SR
Ji
Shu-xing
SX
D000293:Adolescent; D000328:Adult; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D055513:Connective Tissue Growth Factor; D005260:Female; D015870:Gene Expression; D006801:Humans; D006909:Hydroxyproline / Q000096:biosynthesis* / Q000235:genetics; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D007627:Keloid / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor; D006909:Hydroxyproline