A pair of rosette glands in the embryo and zoeal larva of an estuarine crab Sesarma haematocheir, and classification of the tegumental glands in the embryos of other crabs.
A pair of rosette glands (one of the tegumental glands in crustaceans) is present at the root of the dorsal spine of the thorax in mature embryos of the estuarine crab Sesarma haematocheir. Each rosette gland is spherical, 45-50 microm in diameter. This gland consists of three types of cells: 18-20 secretory cells, one central cell, and one canal cell. The secretory cells are further classified into two types on the basis of the morphology of secretory granules. There are 17-19 a cells, and only one b cell per rosette gland. An a cell contains spherical secretory granules of 2-3 microm in diameter. The granules are filled with highly electron-dense materials near the nucleus but have lower electron-density near the central cell. The secretory granules contained in the b cell have an irregular shape and are 1-1.5 microm in diameter. The density of the materials in the granules is uniform throughout the cytoplasm. The secretory granules contained in both the a and b cells are produced by the rough endoplasmic reticulum. Materials in the granules are exocytotically discharged into the secretory apparatus inside the secretory cell, sent to the extracellular channels in the central cell, and secreted through the canal cell. The rosette gland can be distinguished from the epidermal cells 2 weeks after egg-laying and the gland matures just before hatching. Materials produced by this gland are secreted after hatching and secretion continues through five stages of zoeal larvae. These rosette glands were never found in the megalopal larva. Rosette glands are found in the embryos of Sesarma spp. and Uca spp. In other crabs, tegumental glands are also found at the same position as in the embryo of S. haematocheir, but the fine structure of their glands is largely different from that of the rosette gland. On the basis of the morphology of secretory cells (a-g cell types), the tegumental glands of a variety of crab embryos can be classified into four types, including rosette glands (type I-IV). The function of these tegumental glands is not yet known, but different types of the gland seem to reflect the phylogeny of the crabs rather than differences of habitat.
Ikeda
Hideki
H
Laboratory of Behavior and Evolution, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Hirano
Yuriko
Y
Saigusa
Masayuki
M
D000818:Animals; D012634:Bodily Secretions / Q000502:physiology; D003386:Brachyura / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D002454:Cell Differentiation / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000196:embryology* / Q000648:ultrastructure; D047108:Embryonic Development; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005088:Exocrine Glands / Q000378:metabolism / Q000648:ultrastructure*; D005089:Exocytosis / Q000502:physiology; D005260:Female; D007814:Larva / Q000254:growth & development* / Q000648:ultrastructure; D038081:Organogenesis / Q000502:physiology; D022142:Secretory Vesicles / Q000378:metabolism / Q000648:ultrastructure; D013045:Species Specificity