title: First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis.
abstract: Since the invading trophoblast represents a semi-allograft, it should be rejected by the mother. It has, therefore, been postulated that during normal pregnancy the trophoblast evades the maternal immune system though the establishment of immune privilege by triggering the death of activated lymphocytes which may be sensitized to paternal alloantigens. Such peripheral tolerance may be directed through the Fas/Fas ligand (FasL) apoptotic pathway and mediated by FasL expressed by the trophoblast. However, in vivo studies show that membrane-associated expression of FasL may instead promote allograft rejection, rather than protection. The aim of this study was to determine if there is a role for FasL in trophoblast immune privilege. In this study, we demonstrate that isolated first trimester trophoblast cells lack membrane-associated FasL, but express a cytoplasmic form in association with a specialized secretory lysosomal pathway. Furthermore, this intracellular FasL is constitutively secreted by trophoblast cells via the release of microvesicles. Following disruption of these microvesicles, the whole 37 kDa secreted FasL is able to induce T-cell death by apoptosis through activation of the Fas pathway. Therefore, we propose that secretion of FasL may be one mechanism by which trophoblast cells promote a state of immune privilege and, therefore, protect themselves from maternal immune recognition.
authors.affiliation: Department of Obstetrics and Gynecology, Yale University, School of Medicine, 333 Cedar Street, FMB 301, New Haven, CT 06520, USA.
authors.forename: Vikki M
authors.initials: VM
authors.lastname: Abrahams
authors.forename: Shawn L
authors.initials: SL
authors.lastname: Straszewski-Chavez
authors.forename: Seth
authors.initials: S
authors.lastname: Guller
authors.forename: Gil
authors.initials: G
authors.lastname: Mor
mesh_terms: D017209:Apoptosis; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins