title: Localisation of exogenous surfactants in cell membranes in the air-blood barrier: rat model.
abstract: The use of exogenous surfactants has been introduced into the therapy of patients of different ages. Much better results have been obtained in the treatment of respiratory distress syndrome with surfactants enriched with surfactant proteins. In the following study we used protein-containing surfactants (survanta and curosurf). The aim of the following study was to determine the localisation of artificial surfactants in the lung tissue. Using the Immunogold Technique, biotinylated surfactant proteins were traced in the air-blood barriers. In all lungs the exogenous surfactant was present only in some alveoli. In these parts small areas of atelectasis as well as oedema and transudate accumulation were seen. These changes were less severe after biotinylated curosurf treatment. In electron microscope studies we found surfactant elements in the air-blood barrier and other structures of the alveolar septa. Immunogold studies confirm the presence of biotynylated surfactant in the elements of the air-blood barrier.
authors.affiliation: Department of Clinical Pathomorphology, University of Medical Sciences, Poznań, Poland. gensui@wp.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszałek
authors.forename: Wiesława
authors.initials: W
authors.lastname: Biczysko
authors.forename: Marcin
authors.initials: M
authors.lastname: Wasowicz
authors.forename: Paweł
authors.initials: P
authors.lastname: Surowiak
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.forename: Ewa
authors.initials: E
authors.lastname: Florek
mesh_terms: D000818:Animals; D019921:Biotinylation; D015824:Blood-Air Barrier / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D005122:Exudates and Transudates; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D023421:Models, Animal; D001261:Pulmonary Atelectasis / Q000139:chemically induced / Q000473:pathology; D011663:Pulmonary Surfactants / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms
chemical_list: D011663:Pulmonary Surfactants