Neuropeptides (SP and CGRP) augment pro-inflammatory cytokine production in HSV-infected macrophages.
Neuropeptides are able to modulate cytokine production by macrophages in response to various stimulators. In this study, the effects of neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) on production of pro-inflammatory cytokines TNF and IL-1 beta by macrophages were considered. Mouse peritoneal macrophages were infected with herpes simplex virus type-1 (HSV-1), or remained unstimulated, and cytokine assays were performed after 12 h. IL-1 beta and TNF secretion by unstimulated macrophages have been significantly increased in the presence of SP and CGRP. Each neuropeptide, alone or in coordination with the other, caused significant increase in IL-1 beta and TNF production by HSV-infected mouse peritoneal macrophages. It was concluded that the macrophage-mediated inflammatory response to HSV-1 is enhanced in the presence of these neuropeptides.
Yaraee
Roya
R
Department of Immunology, School of Medicine, Shahed University, Tehran, Iran. yaraee@shahed.ac.ir
Ebtekar
Massoumeh
M
Ahmadiani
Abolhassan
A
Sabahi
Farzaneh
F
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000009:adverse effects* / Q000096:biosynthesis* / Q000378:metabolism; D004338:Drug Combinations; D018259:Herpesvirus 1, Human / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D007249:Inflammation / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000737:chemistry / Q000493:pharmacokinetics; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013373:Substance P / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000737:chemistry / Q000493:pharmacokinetics; D014709:Vero Cells
D016207:Cytokines; D004338:Drug Combinations; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide