title: Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.
abstract: E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
authors.affiliation: Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L. 66450, Mexico.
authors.forename: Naima G
authors.initials: NG
authors.lastname: Cortes-Perez
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez-Padilla
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gruss
authors.forename: Odila
authors.initials: O
authors.lastname: Saucedo-Cárdenas
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Roberto
authors.initials: R
authors.lastname: Montes-de-Oca-Luna
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D005260:Female; D007114:Immunization / Q000379:methods*; D017045:Lactococcus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000954:Antigens, Surface; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16