Identification and characterization of the DdlB, FtsQ and FtsA genes upstream of FtsZ in Bartonella bacilliformis and Bartonella henselae.
Homologues of the cell division protein FtsZ were previously identified in Bartonella bacilliformis and Bartonella henselae. We report herein that ftsZ is located at the distal end of an operon that includes ddlB, ftsQ, and ftsA. These genes code for homologues of D-alanine D-alanine ligase, an enzyme involved in cell wall biosynthesis, and FtsQ, and FtsA, which are involved in cell division. The DdlB, FtsQ, and FtsA proteins from Bartonella species are most homologous to proteins in closely related species from the Order Rhizobiales, such as Brucella sp., Agrobacterium tumefaciens, and M. loti. The organization of the genes within the ddlB-ftsZ operon of B. bacilliformis and B. henselae (5'ddlB-ftsQ-ftsA-ftsZ 3') is similar to that of Mesorhizobium loti and Escherichia coli. We report the localization of three promoter regions within the ddlB-ftsA sequence of B. bacilliformis that may enhance the transcription of ftsZ mRNA. A promoter region was also identified upstream of the ddlB gene.
Fiskus
Warren
W
Department of Biology, Georgia State University, Atlanta, GA 30302-4010, USA.
Padmalayam
Indira
I
Kelly
Tim
T
Guibao
Christina
C
Baumstark
Barbara R
BR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D045503:Bartonella bacilliformis / Q000235:genetics*; D018416:Bartonella henselae / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D003598:Cytoskeletal Proteins; D004269:DNA, Bacterial / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics; D012230:Rhizobiaceae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C065103:FtsA protein, Bacteria; C489310:FtsA protein, E coli; C068023:FtsQ protein, E coli; C071525:FtsZ protein, Bacteria; D008565:Membrane Proteins; D010453:Peptide Synthases; C019638:D-alanylalanine synthetase