FT-IR study of heterologous protein expression in recombinant Escherichia coli strains.
Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.
Ami
Diletta
D
INFM UdR Milano-Bicocca, Department of Biotechnologies and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
Bonecchi
Loredana
L
Calì
Simona
S
Orsini
Gaetano
G
Tonon
Giancarlo
G
Doglia
Silvia Maria
SM
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000032:analysis* / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000096:biosynthesis; D011994:Recombinant Proteins; D017550:Spectroscopy, Fourier Transform Infrared
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins