title: Highly sensitive gas chromatographic determination of ethanol in human urine samples.
abstract: In order to evaluate recent alcohol consumption, a very sensitive and specific gas chromatographic method for ethanol determination in human urine samples was developed. The non-invasive method was performed without any pretreatment and carried out on a Stabilwax capillary column, 30 m x 0.53 mm x 1.0 microm film thickness. Helium was used as carrier gas with a constant inlet pressure of 27.72 kPa (0.277 bar) and a flame ionization detector (FID). Quantification was performed with the use of acetonitrile as an internal standard (IS). The calibration curve was linear throughout the concentration range from 0.5 to 500 mg/l. The calculated intra- and inter-day coefficients of variation were below 8%. A clear chromatographic separation of ethanol from methanol, acetone, 1-propanol and 2-propanol was achieved.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases and Hepatology, Medizinische Poliklinik, University of Wuerzburg, Josef-Schneider Strasse 2, D-97080, Wuerzburg, Germany. zilly_m@klinik.uni-wuerzburg.de
authors.forename: Michael
authors.initials: M
authors.lastname: Zilly
authors.forename: Peter
authors.initials: P
authors.lastname: Langmann
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lenker
authors.forename: Verena
authors.initials: V
authors.lastname: Satzinger
authors.forename: Diana
authors.initials: D
authors.lastname: Schirmer
authors.forename: Hartwig
authors.initials: H
authors.lastname: Klinker
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D000431:Ethanol / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000431:Ethanol