DAPI derivative: a fluorescent DNA dye that can be covalently attached to biomolecules.
The preparation of a DAPI (4',6-diamidino-2-phenylindole) derivative is described. The resulting derivative retains the fluorogenic property upon binding to double-stranded DNA. Its ability for bioconjugation through amide linkage is demonstrated.
Li
Min
M
Roche Diagnostics Corporation, 9115 Hague Road, Indianapolis, IN 46250, USA. minli88@att.net
Wu
Robert S
RS
Tsai
Jane S C
JS
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007211:Indoles; D012710:Serum Albumin, Bovine; C007293:DAPI