A smart bioconjugate of alginate and pectinase with unusual biological activity toward chitosan.
The commercial preparation of pectinase (Pectinex Ultra SP-L) was conjugated to alginate by noncovalent interactions by employing 1% alginate during the conjugation protocol. The optimum "immobilization efficiency" was 0.76. The pH optimum and the thermal stability of the enzyme remained unchanged upon conjugation with alginate. The soluble bioconjugate showed a 3-fold increase in V(max)/K(m) as compared to the free enzyme when the smart biocatalyst was used for chitosan hydrolysis. Time course hydrolysis of chitosan thus showed higher conversion of chitosan into reducing oligosaccharides/sugars. The smart bioconjugate could be reused five times without any detectable loss of chitosanase activity.
Sardar
Meryam
M
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Roy
Ipsita
I
Gupta
Munishwar N
MN
D000464:Alginates / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D046911:Macromolecular Substances; D010368:Pectins / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D046911:Macromolecular Substances; D002686:Chitin; D010368:Pectins; D020723:Glucuronic Acid; D048271:Chitosan; D011096:Polygalacturonase; C003181:polygalacturonic acid