[Study on mechanical compression regulating the proliferation of young growing rat masticatory myocyte in vitro].
OBJECTIVE
To establish an experimental model which was the masticatory myocyte culture and force stimulation in vitro and study the proliferation changes of cultured masticatory myocytes caused by mechanical compression in young rat.


METHODS
Flow cytometry (FCM) was used to examine the changes of cellular DNA content and cell cycles of cultured masticatory myocytes.


RESULTS
The DNA content in experimental groups was higher than that in control. After the compressive force was applied for 2 hours, the proliferation index (PI) in experimental group became higher than that in control. Under a continuous pressure for 4 hours, the PI in 2,000 mu strain group reached the maximum (48.9%) but the PI in 4,000 mu strain group reached the minimum (39.0%).


CONCLUSIONS
The proliferation of masticatory myocytes from young rat increased under certain force and certain period of time, but decreased if the force applied was overloaded.
Chen
Kaiyun
K
Department of Orthodontics, Oral Hospital of Guangdong Province, Guangzhou 510280, China.
Luo
Songjiao
S
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D003799:Dental Stress Analysis; D005434:Flow Cytometry; D008410:Masticatory Muscles / Q000166:cytology* / Q000502:physiology; D032342:Muscle Cells / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical
D004247:DNA