title: Biogenetic studies in Syringa vulgaris L.: bioconversion of (18)O(2H)-labeled precursors into lilac aldehydes and lilac alcohols.
abstract: Syringa vulgaris L. inflorescences, petals, and chloroplasts, isolated from lilac flower petals, were fed with aqueous solutions of (18)O-labeled linalool and [5,5-(2)H(2)]-deoxy-d-xylose (DOX). The chloroplasts of lilac flower petals were isolated after feeding experiments with labeled precursors. Volatiles from the chloroplasts were extracted by stir bar sorptive extraction (SBSE) and analyzed using enantioselective multidimensional gas chromatography-mass spectrometry (enantio-MDGC-MS). Feeding experiments with DOX indicate that the novel mevalonate-independent 1-deoxy-d-xylose 5-phosphate/2C-methyl-d-erythritol 4-phosphate (DOX/MEP) is the decisive pathway of lilac aldehyde and lilac alcohol, respectively. Bioconversion of [(18)O]linalool into lilac aldehyde and lilac alcohol during in vivo feeding experiments was monitored, and the metabolic pathways are discussed.
authors.affiliation: Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439 Frankfurt Main, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Burkhardt
authors.forename: Armin
authors.initials: A
authors.lastname: Mosandl
mesh_terms: D000080462:Acyclic Monoterpenes; D000438:Alcohols / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D003903:Deuterium; D035264:Flowers / Q000378:metabolism / Q000648:ultrastructure; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D039821:Monoterpenes / Q000378:metabolism; D010103:Oxygen Isotopes; D031662:Syringa / Q000378:metabolism*; D014835:Volatilization
chemical_list: D000080462:Acyclic Monoterpenes; D000438:Alcohols; D000447:Aldehydes; D039821:Monoterpenes; D010103:Oxygen Isotopes; D003903:Deuterium; C018584:linalool