title: Tissue-engineered urinary bladder wall using PLGA mesh-collagen hybrid scaffolds: a comparison study of collagen sponge and gel as a scaffold.
abstract: OBJECTIVE
  Tissue engineering of the urinary bladder using autologous cells and biodegradable scaffold is a promising method for augmentation. The authors developed 2 hybrid scaffolds by combining poly (DL-lactic-co-glycolic acid; PLGA) mesh for mechanical strength with collagen sponge or gel suitable for cell seeding. The aim of this study was to compare collagen as a scaffold between collagen sponge and gel and to construct a tissue-engineered urinary bladder wall utilizing these hybrid scaffolds.
  
  
  METHODS
  The PLGA mesh-collagen hybrid scaffolds were prepared by introducing collagen sponge or gel into the PLGA knitted mesh. Urothelial and smooth muscle cells were obtained from porcine urinary bladder wall and were cultured in their respective media. The cells were seeded on these hybrid scaffolds. These constructs were analyzed morphologically and immunohistochemically.
  
  
  RESULTS
  The urothelial layer was generated 3 dimensionally by culturing urothelial cells with PLGA mesh and collagen sponge. The smooth muscle layer was constructed by culturing smooth muscle cells with PLGA mesh and collagen gel. And a novel tissue-engineered urinary bladder wall was constructed laminating the urothelial and smooth muscle layers.
  
  
  CONCLUSIONS
  Ex vivo construction of urinary bladder wall using hybrid scaffolds prepared by combining PLGA mesh with collagen sponge or gel was successful. This tissue-engineered urinary bladder wall allows easy handling and may become a promising tool for bladder augmentation.
authors.affiliation: Department of Pediatric Surgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Komuro
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
authors.forename: Setsuko
authors.initials: S
authors.lastname: Kaneko
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tateishi
authors.forename: Michio
authors.initials: M
authors.lastname: Kaneko
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D005782:Gels; D019344:Lactic Acid; D032389:Myocytes, Smooth Muscle; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D013552:Swine; D023822:Tissue Engineering; D001743:Urinary Bladder; D019459:Urothelium / Q000166:cytology*
chemical_list: D005782:Gels; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen