Deletion of Mycobacterium tuberculosis sigma factor E results in delayed time to death with bacterial persistence in the lungs of aerosol-infected mice.
The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a significant reduction of bacterial numbers in lungs.
Ando
Masaru
M
Department of Medicine, Center for Tuberculosis Research, Johns Hopkins School of Medicine, Baltimore, Maryland 21231-1001, USA.
Yoshimatsu
Tetsuyuki
T
Ko
Chiew
C
Converse
Paul J
PJ
Bishai
William R
WR
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D015169:Colony Count, Microbial; D017353:Gene Deletion; D018869:Heat-Shock Response; D006801:Humans; D008168:Lung / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016513:Mice, SCID; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000472:pathogenicity*; D012808:Sigma Factor / Q000235:genetics*; D016019:Survival Analysis; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000401:mortality*
D000336:Aerosols; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors