Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli.
PprI, a newly identified gene switch responsible for extreme radioresistance of Deinococcus radiodurans, plays a central regulatory role in multiple DNA damage repair and protection pathways in response to radiation stress [Biochem. Biophy. Res. Commun. 306 (2003) 354]. To evaluate whether PprI also functions in the radioresistance in other organisms, D. radiodurans PprI protein (Deira-PprI) was expressed in Escherichia coli. The complemented E. coli strain showed an increase of approximately 1.6-fold radioresistance with a high dose of gamma irradiation. Immunoblotting assays showed that the expression of Deira-PprI in E. coli resulted in a significant increase in RecA protein expression following high dose ionizing radiation. The expression of Deira-PprI protein also significantly enhanced the scavenging ability of free radicals by inducing the enzymatic activity of KatG. These results indicate that exogenous expression of Deira-PprI promotes DNA repair and protection pathways and enhances the radioresistance of E. coli.
Gao
Guanjun
G
Institute of Nuclear-Agricultural Science, Zhejiang University, 310029, China.
Tian
Bing
B
Liu
Lili
L
Sheng
Duohong
D
Shen
Binghui
B
Hua
Yuejin
Y
D002374:Catalase / Q000378:metabolism; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D034301:Deinococcus / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism / Q000528:radiation effects*; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays; D015870:Gene Expression; D015151:Immunoblotting; D010957:Plasmids / Q000378:metabolism; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D011938:Rec A Recombinases / Q000378:metabolism; D015996:Survival Rate; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
D005609:Free Radicals; D015534:Trans-Activators; D002374:Catalase; D011938:Rec A Recombinases