A combined atomic force microscopy and molecular dynamics simulation study on a plastocyanin mutant chemisorbed on a gold surface.
A mutant of copper plastocyanin, covalently bound to an Au (111) surface through an engineered disulfide bridge, was investigated in aqueous medium by atomic force microscopy (AFM) and molecular dynamics (MD) simulations. Tapping-mode AFM images revealed adsorption of single molecules which are homogeneously distributed over the substrate and strongly bound to gold and display uniform lateral size. A statistical analysis of the height of the macromolecules on the gold substrate evidenced a distribution around a mean value consistent with that expected from the crystallographic data and with a relatively large standard deviation. A 10-ns classical MD simulation of mutated plastocyanin, hydrated by a layer of water, covalently bound to a gold surface by one or two sulfur atoms, was performed. The simulations indicate that the bound protein retains, in both cases, its overall tertiary structure during the dynamic evolution. Moreover, the macro-molecule can assume different orientations with respect to the gold substrate, which give rise to a distribution of heights on the gold substrate. Experimental and MD simulation results are compared and discussed in connection with the topological and dynamical properties of the protein system.
Bizzarri
Anna Rita
AR
Biophysics and Nanoscience Group, INFM, Dipartimento di Scienze Ambientali Università della Tuscia, Largo dell'Università, 01100 Viterbo, Italy.
Bonanni
Beatrice
B
Costantini
Giulio
G
Cannistraro
Salvatore
S
D000327:Adsorption; D001665:Binding Sites; D003198:Computer Simulation; D003545:Cysteine / Q000235:genetics; D003627:Data Interpretation, Statistical; D004220:Disulfides / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D008968:Molecular Conformation; D009154:Mutation; D010970:Plastocyanin / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D013499:Surface Properties
D004220:Disulfides; D011506:Proteins; D006046:Gold; D010970:Plastocyanin; D003545:Cysteine