Modulation of intracellular Cl- homeostasis by lectin-stimulation in Jurkat T lymphocytes.
We investigated changes in intracellular Cl(-) concentration ([Cl(-)](i)) during lectin-induced activation and proliferation in human Jurkat T lymphocytes. [Cl(-)](i) was measured using Cl(-) fluorescence dye (N-(6-methoyquinolyl) acetoxy-acetyl-ester, MQAE) methods. Lectins, phytohemagglutinin and concanavalin A, dose-dependently increased [Cl(-)](i) and triggered intracellular Cl(-) oscillation in human Jurkat T lymphocytes. However, some mitochondria metabolism inhibitors, such as m-chlorocarbonylcyanide phenylhydrazone (CCP) and 2,4-dinitrophenol, increased [Cl(-)](i) without triggering any Cl(-) oscillation. Furthermore, both lectins and metabolism inhibitors-induced elevation in [Cl(-)](i) were blocked by removal of extracellular Cl(-) from perfusion solution or by application of anthracene-9-carboxylate, a blocker of Cl(-) channels. Since an extracellular Cl(-)-free condition and application of 9-AC also inhibited PHA-induced proliferation, we suggested that elevation of [Cl(-)](i) via activation of Cl(-) channels and increase in incidence of Cl(-) oscillation would play an important role in modulation of Jurkat T cell activation and proliferation.
Lai
Zhong-Fang
ZF
Department of Cell and Biological Pharmacology, Graduate School of Medical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-0811, Japan. lai-zf@gpo.kumamoto-u.ac.jp
Chen
Yu-Zhen
YZ
Nishi
Katsuhide
K
D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000502:physiology*; D019169:Jurkat Cells; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D018118:Chloride Channels; D037102:Lectins; D010835:Phytohemagglutinins; D003208:Concanavalin A