Enhanced phagocytosis of Candida species mediated by opsonization with a recombinant human antibody single-chain variable fragment.
Specific antibody opsonization significantly enhances the level of phagocytosis of Candida in the absence of complement. Furthermore, we have described a system using a recombinant human antibody single-chain variable fragment that allows a comparative study of phagocytosis of multiple Candida species opsonized via a common antigen.
Wellington
Melanie
M
Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
Bliss
Joseph M
JM
Haidaris
Constantine G
CG
D000908:Antibodies, Fungal / Q000235:genetics / Q000276:immunology; D002175:Candida / Q000145:classification* / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000378:metabolism*; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000276:immunology*
D000908:Antibodies, Fungal; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D009895:Opsonin Proteins; D011994:Recombinant Proteins