title: Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMPhi cells from rats.
abstract: OBJECTIVE
  To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.
  
  
  METHODS
  With carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.
  
  
  RESULTS
  Decoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.
  
  
  CONCLUSIONS
  Decoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats.
authors.affiliation: Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.forename: Hua-ping
authors.initials: HP
authors.lastname: Liang
authors.forename: Feng-lin
authors.initials: FL
authors.lastname: Lu
authors.forename: Xiang
authors.initials: X
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: An
authors.forename: Tian-zhi
authors.initials: TZ
authors.lastname: Hao
authors.forename: Fu-lLong
authors.initials: FL
authors.lastname: Wang
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10