Cytapheresis with a filter for selective removal of CD4+ T cells in experimental autoimmune encephalomyelitis.
Experimental autoimmune encephalomyelitis (EAE) is a major animal model of human multiple sclerosis (MS). CD4+ T cells are thought to play a pivotal role in the pathogenesis of EAE and MS. In order to investigate the depletion of CD4+ T cells from the systemic circulation as an effective strategy for the treatment of MS, we performed extracorporeal CD4+ T cell adsorption, using a filter to which anti-CD4+ antibody is immobilized as a ligand, in adoptively transferred EAE. Rats treated with CD4+ T cell removal filter (CD4RF) exhibited milder clinical signs of EAE and earlier recovery than those receiving sham treatment. Moreover, the thymic cells from EAE rats treated with CD4RF exhibited a suppressed proliferative response and IFN-gamma production to myelin basic protein. These results suggest that depletion of CD4+ T cells from the systemic circulation by extracorporeal treatment is a potentially useful strategy for treatment of acute phase and relapsing MS.
Nakane
Shunya
S
Department of Neurology, Kawatana National Hospital, Nagasaki, Japan.
Matsuo
Hidenori
H
Goto
Hirofumi
H
Yoshinaga-Matsumoto
Megumi
M
Ohtsuru
Izumi
I
Ichinose
Katsuhiro
K
Onodera
Hirokazu
H
Yoshida
Makoto
M
Shibuya
Noritoshi
N
D000208:Acute Disease; D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002460:Cell Line; D016238:Cytapheresis / Q000295:instrumentation / Q000379:methods*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D007371:Interferon-gamma / Q000378:metabolism; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000276:immunology / Q000473:pathology / Q000628:therapy; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D004676:Myelin Basic Protein; D007371:Interferon-gamma