The fusion peptide of simian immunodeficiency virus and the phase behaviour of N-methylated dioleoylphosphatidylethanolamine.
Temperature-scan X-ray scattering was used to study the effect of the fusion peptide of simian immunodeficiency virus (SIV) on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine (DOPE-Me), in the presence and absence of one or both of the fusion inhibitors carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC). Using X-ray diffraction at stations 2.1 and 8.2 of the Synchrotron Radiation Source at Daresbury Laboratory, UK, the structure of multilamellar vesicles (MLVs) was probed as the temperature was raised from 20 to 90 degrees C. The results are compared to those of similar studies, reported earlier, that used the fusion peptide of feline leukaemia virus (FeLV) which, at 28 amino acid residues in length, is considerably longer than the SIV peptide (12 amino acid residues). We interpret the results within the framework of current understanding of membrane fusion, and demonstrate how observed lipid polymorphism might describe the fusion process.
Harroun
Thad A
TA
Department of Preclinical Veterinary Sciences, Royal School of Veterinary Studies, University of Edinburgh, Summerhall, EH9 1QH Scotland, Edinburgh, UK.
Balali-Mood
Kia
K
Gourlay
Ian
I
Bradshaw
Jeremy P
JP
D015686:Gene Products, env / Q000737:chemistry; D007783:Lactones / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D046911:Macromolecular Substances; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008745:Methylation; D008968:Molecular Conformation; D044367:Phase Transition; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011485:Protein Binding; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry; D013696:Temperature; D014759:Viral Envelope Proteins / Q000737:chemistry; D014760:Viral Fusion Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
D015686:Gene Products, env; D007783:Lactones; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D010714:Phosphatidylethanolamines; D015682:Retroviridae Proteins, Oncogenic; C087575:SIV envelope glycoprotein gp160; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C078678:p15E protein, Murine leukemia virus; C094877:dioleoyl phosphatidylethanolamine; C054164:lipstatin