[Comparison of synthesizing Collagen I and III in periodontal ligament cells and gingival fibroblasts].
OBJECTIVE
To investigate the different characteristics of periodontal ligament cells and gingival fibroblasts in synthesizing Collagen I and III.


METHODS
Periodontal ligament cells and gingival fibroblasts were cultured from the same patient and detected for expression of Collagen I and III by immunocytochemical methods.


RESULTS
The immunocytochemical staining showed Collagen I and III were positive in periodontal ligament cells, and mild positive in gingival fibroblasts. Statistical analysis suggested the difference between gingival fibroblasts and periodontal ligament cells.


CONCLUSIONS
There were differences between the periodontal ligament cells and gingival fibroblasts in synthesizing or secreting of Collagen matrix.
Wang
Yan
Y
School of Stomatology, Shandong University, Jinan 250012, China. wangyan1965@yahoo.com.cn
Wang
Shuo-zhi
SZ
Fan
Ming-wen
MW
Yang
Pi-shan
PS
Li
Shu
S
D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000096:biosynthesis*; D024061:Collagen Type III / Q000032:analysis / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010513:Periodontal Ligament / Q000378:metabolism*
D024042:Collagen Type I; D024061:Collagen Type III