title: Three-dimensional MRI of cerebral projections in rat brain in vivo after intracortical injection of MnCl2.
abstract: In this study we investigated the potential of in vivo MRI detection of axonal Mn2+ transport for tracing neuronal projections originating in the sensorimotor cortex in healthy and lesioned rat brains. Special attention was given to the potential of visualizing neuronal sprouting of central nervous system across the midline. After injecting unchelated MnCl2 into the forelimb area of sensorimotor cortex of 18 healthy and 10 lesioned rats corticofugal projections could be traced through the internal capsule to the cerebral peduncle and the pyramidal decussation. Although the neuronal tract was visible as early as 6 h after MnCl2 injection, best contrast was achieved after 24-48 h. Beside the cortico-spinal tract, the cortico-thalamic fibres were also visualized by anterograde Mn2+ transport. Cortico-striatal fibres were partially masked by the very high signal near the MnCl2 injection site but could be discerned as well. Slight, diffuse signal enhancement of cortical tissue contralateral to the MnCl2 injection site in healthy rat brains suggests interhemispheric connections or passive diffusion of Mn2+. However, enhanced fibre tract contrast connecting both hemispheres was visible 16 weeks after onset of focal photothrombotic cortical injury. In conclusion our study has shown that we were able to visualize reproducibly the main descending corticofugal projections and interhemispheric connections by non-invasive MRI after localized injection of MnCl2. The appearance of interhemispheric Mn2+-enhanced fibres after photothrombotic focal injury indicates that the method may bear potential to follow non-invasively gross plastic changes of connectivity in the brain after injury.
authors.affiliation: Novartis Pharma AG, Preclinical Research, PO Box, CH-4002 Basel, Switzerland. peter.allegrini@pharma.novartis.com
authors.forename: Peter R
authors.initials: PR
authors.lastname: Allegrini
authors.forename: Christoph
authors.initials: C
authors.lastname: Wiessner
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D020767:Intracranial Thrombosis / Q000378:metabolism / Q000473:pathology*; D015636:Magnesium Chloride / Q000008:administration & dosage / Q000493:pharmacokinetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008845:Microinjections / Q000379:methods; D009044:Motor Cortex / Q000378:metabolism / Q000473:pathology*; D009434:Neural Pathways / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D013003:Somatosensory Cortex / Q000378:metabolism / Q000473:pathology*; D014018:Tissue Distribution
chemical_list: D015636:Magnesium Chloride