title: Degradation of ferric EDTA by Burkhol cepacia.
abstract: EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5-15 microm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4 + (NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH 7.0, 30 degrees C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d-1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.
authors.affiliation: Graduate of Engineering Science, Technology School (Doctoral Program), National YunLin University of Science and Technology, Touliu, Yunlin 640, Taiwan. fanghy@es.yuntech.edu.tw
authors.forename: Hung-Yuan
authors.initials: HY
authors.lastname: Fang
authors.forename: Shih-Chin
authors.initials: SC
authors.lastname: Chen
authors.forename: Szu-Lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004492:Edetic Acid / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D000644:Quaternary Ammonium Compounds; D004492:Edetic Acid; C019179:Fe(III)-EDTA