Variability of HXT2 at the protein and gene level among the Saccharomyces sensu stricto group.
Variability of HXT2 at the protein and gene level was investigated among Saccharomyces sensu stricto and other yeast species. Results showed that the HXT2 gene is probably present in yeast genera other than Saccharomyces, suggesting that this gene is widely distributed in the yeast world. Chromosomal analyses indicated the stable location of HXT2 on the same chromosome and with the same copy number throughout the entire sensu stricto group. Results of the immunoblotting assay demonstrated that all strains tested (with the exception of S. cerevisiae DBVPG 6042) exhibited a lower level of Hxt2p expression than that shown by laboratory wild-type. Moreover, Hxt2p expression seems to reinforce the taxonomical differences between the two pairs of species (S. cerevisiae and S. paradoxus vs. S. pastorianus and S. bayanus) within the sensu stricto group of the genus of Saccharomyces that also reflect their different ecological niche.
Selvi
Sabrina
S
Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, Università di Perugia, via Borgo XX Giugno 74, 06100, Perugia, Italy.
Cardinali
Gianluigi
G
Ciani
Maurizio
M
D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000032:analysis; D014644:Genetic Variation; D051246:Glucose Transport Proteins, Facilitative; D008565:Membrane Proteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012440:Saccharomyces / Q000145:classification / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D004271:DNA, Fungal; D051246:Glucose Transport Proteins, Facilitative; C084498:HXT2 protein, S cerevisiae; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins