Optimization of cyclodextrin production from sago starch.
Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optimum pH and temperature for CD production from sago starch were found to be in the ranges of 4.5-5.0 and 55-60 degrees C, respectively. beta-CD was the predominant product, constituting 65% of all CD products. The beta-CD produced using partially purified and crude CGTase were compared and found to have no significant difference in yield and productivity. The appropriate proportion of CGTase to sago starch for beta-CD production was determined by response surface methodology. The most appropriate enzyme:substrate ratio was 50 U g sago starch(-1) CGTase and 60 g l(-1) sago starch.
Charoenlap
Nisanart
N
Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Payathai, Bangkok 10400, Thailand.
Dharmsthiti
Saovanee
S
Sirisansaneeyakul
Sarote
S
Lertsiri
Sittiwat
S
D000704:Analysis of Variance; D001407:Bacillus / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D029784:Cycas / Q000737:chemistry*; D003505:Cyclodextrins / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012044:Regression Analysis; D013213:Starch / Q000378:metabolism*; D013696:Temperature
D003505:Cyclodextrins; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase