title: Cry29A and Cry30A: two novel delta-endotoxins isolated from Bacillus thuringiensis serovar medellin.
abstract: Two novel crystal protein genes from a highly mosquitocidal Bacillus thuringiensis serovar medellin strain were cloned and sequenced. The corresponding proteins, Cry29A and Cry30A, were nontoxic when tested individually against the mosquito species bioassayed (Aedes aegypti, Culex pipiens and Anopheles stephensi). However, Cry29A synergized the toxicity of Cry11Bb against Aedes aegypti by a four-fold factor.
authors.affiliation: Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur, Paris, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Juárez-Pérez
authors.forename: Manuel
authors.initials: M
authors.lastname: Porcar
authors.forename: Sergio
authors.initials: S
authors.lastname: Orduz
authors.forename: Armelle
authors.initials: A
authors.lastname: Delécluse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D015139:Blotting, Southern; D009033:Culicidae / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C115468:CRY11BB protein, Bacillus thuringiensis; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis