The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest.
The transcription factor CCAAT enhancer-binding protein alpha (C/EBPalpha) is a tumor suppressor in myeloid cells and inhibits proliferation in all cell types examined. C/EBPalpha interacts with the SWI/SNF chromatin-remodeling complex during the regulation of differentiation-specific genes. Here we show that C/EBPalpha fails to suppress proliferation in SWI/SNF defective cell lines after knock-down of SWI/SNF core components or after deletion of the SWI/SNF interaction domain in C/EBPalpha, respectively. Reconstitution of SWI/SNF function restores C/EBPalpha-dependent proliferation arrest. Our results show that the anti-proliferation activity of C/EBPalpha critically depends on components of the SWI/SNF core complex and suggest that the functional interaction between SWI/SNF and C/EBPalpha is a prerequisite for proliferation arrest.
Müller
Christine
C
Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, D-13092 Berlin, Germany.
Calkhoven
Cornelis F
CF
Sha
Xiaojing
X
Leutz
Achim
A
D000818:Animals; D015153:Blotting, Western; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D008957:Models, Genetic; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000378:metabolism; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D013997:Time Factors; D014162:Transfection
D022763:CCAAT-Enhancer-Binding Protein-alpha; D002843:Chromatin; D020033:Protein Isoforms