Analysis of the RNA helicase A gene in human lung cancer.
In this study we investigated the RNA helicase A (RHA) gene localized on chromosome 1q25 in 15 lung cancer cell lines, 54 primary carcinomas, bronchial epithelial cells and normal lung. Both RT-PCR and real-time RT-PCR showed that RHA is over-expressed in tumour samples compared to normal lung tissues (p=0.01). There was a tendency for higher expression levels in small cell lung cancer compared to non-small cell carcinomas. Over-expression of Y-162 correlated with high grade tumours (p=0.023). However, there was no correlation with tumour stage and survival. Our observations are compatible with the role of RHA in lung cancer development.
Wei
Xiao
X
Institute of Pathology, University Hospital Charité, Berlin, Germany.
Pacyna-Gengelbach
Manuela
M
Schlüns
Karsten
K
An
Qian
Q
Gao
Yanning
Y
Cheng
Shujun
S
Petersen
Iver
I
D001324:Autoantigens / Q000235:genetics*; D015152:Blotting, Northern; D002460:Cell Line; D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D009363:Neoplasm Proteins; D009693:Nucleic Acid Hybridization / Q000379:methods; D020365:RNA Helicases / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001324:Autoantigens; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C098487:DHX9 protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases