Bacterial biofilm in seawater: cell surface properties of early-attached marine bacteria.
The development of antifouling strategies in seawater requires knowledge of the physico-chemical properties of the cell surfaces of early adherent bacteria. The hydrophilic, electrostatic and the Lewis acid-base cell surface properties of eleven marine bacteria were characterized. Although these bacteria adhered to a hydrophilic support immersed for 3 and 6 h, they presented various physico-chemical properties. Eleven strains possessed a hydrophilic surface and five a hydrophobic surface. Although the majority of the bacteria presented an electron-donating character, some could not generate Lewis acid-base interactions with the support. On the other hand, all strains possessed an isoelectric point ranging from 2.2 to 3.4 and were negatively charged at the pH of seawater. Hydrophilicity was a preponderant property among these bacteria, but other properties should not be ignored. The development of new antifouling paints must take account all the possible interaction levels used by the bacteria to adhere to an immersed surface.
Grasland
Beatrice
B
Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne-Sud, F-56321 Lorient (EA 2594), France.
Mitalane
Jalila
J
Briandet
Romain
R
Quemener
Eric
E
Meylheuc
Thierry
T
Linossier
Isabelle
I
Vallee-Rehel
Karine
K
Haras
Dominique
D
D001419:Bacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D017931:DNA Primers; D004586:Electrophoresis; D057927:Hydrophobic and Hydrophilic Interactions; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D012997:Solvents / Q000378:metabolism; D013499:Surface Properties; D000267:Tissue Adhesions
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012997:Solvents