Occurrence of hepatitis viruses in wild-born non-human primates: a 3 year (1998-2001) epidemiological survey in Gabon.
Hepatitis B and C infections are endemic in human population in central Africa, particularly in Gabon. The aim of this study was to determine the prevalence of hepatitis B virus (HBV) and eventual occurrence of hepatitis C virus (HBC)-related strains in a variety of wild-born non-human primates living in Gabon and Congo. Plasma samples were screened for HBV and HCV markers. A non-invasive method of DNA extraction from faeces followed by specific HBV-DNA amplification was developed to study this infection in wild troops of chimpanzees and gorillas. No HCV infection in non-human primates, wild-born or captive, was detected among 596 samples tested. No HBV infection could be detected in samples tested and obtained from Cercopithecidae. In contrast, 14.7 and 42.2% of wild-born chimpanzees in Gabon and Congo were infected with HBV or had evidence of past HBV infection. At Centre International de Recherches Médicales (CIRMF) Primate Centre, 32.1% of chimpanzees and gorillas were HBV positive or had evidence of past infection. In the cases with past infection, 5.9% wild-born and 8.3% at CIRMF harboured HBV-DNA despite the presence of neutralizing HbsAb. Together with previous findings, we confirm the high HBV prevalence not only in humans but also in chimpanzees and gorillas in Gabon and Congo.
Makuwa
M
M
Centre International de Recherches Médicales, Franceville, Gabon. mmakuwa@yahoo.com
Souquière
S
S
Telfer
P
P
Leroy
E
E
Bourry
O
O
Rouquet
P
P
Clifford
S
S
Wickings
E J
EJ
Roques
P
P
Simon
F
F
D000818:Animals; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005681:Gabon / Q000453:epidemiology; D016174:Hepacivirus / Q000276:immunology; D006509:Hepatitis B / Q000453:epidemiology / Q000662:veterinary*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018419:Primate Diseases / Q000453:epidemiology*; D011323:Primates / Q000276:immunology / Q000821:virology*
D017931:DNA Primers