Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissues.
Ablation of GLUT4 in adipose tissues results in whole body insulin resistance and high-fat feeding down-regulates GLUT4 mRNA in white adipose tissues. Previous studies demonstrated that adipose tissue specific element(s) (ASE) of the murine GLUT4 gene is located between -551 and -442 relative to transcription start site and that high-fat responsive element(s) (HFRE) for down-regulation of the GLUT4 gene is located between bases -1001 and -442. To further characterize these regulatory elements, the regulation of GLUT4 minigenes containing -701, -551, and -506 bp of 5(')-flanking region was studied in transgenic mice. GLUT4 minigene mRNA from -506 transgenic mice did not express in adipose tissues, indicating that ASE located within 45 bp is located between bases -551 and -506. An 80-kDa of nuclear DNA binding protein was found to bind to a -TCCTCGTGGGAAGCG- element located between bases -551 and -537. High-fat diet feeding down-regulated GLUT4 minigene mRNA in -701 transgenic mice, but not in -551 transgenic mice, indicating that HFRE is located within 150 bp between bases -701 and -551 of the GLUT4 gene and is distinct from ASE.
Miura
Shinji
S
Division of Clinical Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
Tsunoda
Nobuyo
N
Ikeda
Shinobu
S
Kai
Yuko
Y
Ono
Misaki
M
Maruyama
Kayo
K
Takahashi
Mayumi
M
Mochida
Keiji
K
Matsuda
Junichiro
J
Lane
M Daniel
MD
Ezaki
Osamu
O
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D004041:Dietary Fats / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D051275:Glucose Transporter Type 4; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D009124:Muscle Proteins; D009928:Organ Specificity; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014018:Tissue Distribution
D004041:Dietary Fats; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D038621:Regulatory Sequences, Ribonucleic Acid; C495693:Slc2a4 protein, mouse