title: Band-target entropy minimization (BTEM) applied to hyperspectral Raman image data.
abstract: Band-target entropy minimization (BTEM) has been applied to extraction of component spectra from hyperspectral Raman images. In this method singular value decomposition is used to calculate the eigenvectors of the spectroscopic image data set. Bands in non-noise eigenvectors that would normally be used for recovery of spectra are examined for localized spectral features. For a targeted (identified) band, information entropy minimization or a closely related algorithm is used to recover the spectrum containing this feature from the non-noise eigenvectors, plus the next 5-30 eigenvectors, in which noise predominates. Tests for which eigenvectors to include are described. The method is demonstrated on one synthesized Raman image data set and two bone tissue specimens. By inclusion of small amounts of signal that would be unused in other methods, BTEM enables the extraction of a larger number of component spectra than are otherwise obtainable. An improvement in signal/noise ratio of the recovered spectra is also obtained.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Effendi
authors.initials: E
authors.lastname: Widjaja
authors.forename: Nicole
authors.initials: N
authors.lastname: Crane
authors.forename: Tso-Ching
authors.initials: TC
authors.lastname: Chen
authors.forename: Michael D
authors.initials: MD
authors.lastname: Morris
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ignelzi
authors.forename: Barbara R
authors.initials: BR
authors.lastname: McCreadie
mesh_terms: D000368:Aged; D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D019277:Entropy; D005269:Femur / Q000737:chemistry* / Q000473:pathology*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D012886:Skull / Q000737:chemistry* / Q000196:embryology / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*