Functional role of nicotinic acetylcholine receptors in apoptosis in HL-60 cell line.
The subunit composition of nicotinic acetylcholine receptors involved in apoptosis is an ongoing question. HL-60 cells were used in order to investigate the implication of nicotinic acetylcholine receptors in bleomycin-induced apoptosis. We found that bleomycin-induced apoptosis was significantly enhanced by nicotine and was blocked by nicotinic acetylcholine receptor antagonists, including alpha-bungarotoxin, a competitive antagonist of alpha 7 nicotinic receptor. Among the other agonists tested, 3-[2,4-dimethoxybenzylidene]anabaseine (GTS-21)-selective agonist for alpha 7-nicotinic acetylcholine receptor-, but not epibatidine or cytisine, enhanced bleomycin-induced apoptosis. In addition to these results, the detectable presence of alpha 7-mRNA supports a key role of alpha 7-nicotinic acetylcholine receptors in the modulation of the induced apoptosis by nicotine.
Gimonet
Delphine
D
Institut Jean-Godinot, Secteur Prévention, BP 171, 1 Avenue du général Koenig, 51056 Reims Cedex, France.
Grailhe
Regis
R
Coninx
Paul
P
Antonicelli
Frank
F
Haye
Bernard
B
Liautaud-Roger
Françoise
F
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D009538:Nicotine / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000502:physiology*
D011978:Receptors, Nicotinic; D009538:Nicotine