Inducible nitric oxide synthase regulates production of isoprostanes in vivo during chlamydial genital infection in mice.
Urinary nitrite and F(2)-isoprostanes, an index of oxidant stress, were elevated during chlamydial genital infection of mice. Enhancement of urinary nitrite and F(2)-isoprostanes was observed in phagocyte oxidase-deficient mice. Inhibition of inducible nitric oxide synthase reduced isoprostane excretion. We conclude that nitrogen radicals induce F(2)-isoprostane production and excretion during murine chlamydial genital infection.
Ramsey
K H
KH
Department of Microbiology, Chicago College of Osteopathic Medicine, Midwestern University, Downers Grove, Illinois 60515, USA. kramse@midwestern.edu
Sigar
I M
IM
Rana
S V
SV
Gupta
J
J
Holland
S M
SM
Byrne
G I
GI
Morrow
J D
JD
D000818:Animals; D002690:Chlamydia Infections / Q000382:microbiology / Q000503:physiopathology; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites / Q000652:urine*; D010088:Oxidoreductases / Q000235:genetics; D010586:Phagocytes / Q000201:enzymology; D016585:Vaginosis, Bacterial / Q000382:microbiology / Q000503:physiopathology*
D028441:F2-Isoprostanes; D009573:Nitrites; D010088:Oxidoreductases; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse