The simultaneous determination of 1-aminocyclopropane-1-carboxylic acid and cyclopropane-1,1-dicarboxylic acid in Lycopersicum esculentum by high-performance liquid chromatography--electrospray tandem mass spectrometry.
Varying concentrations of cyclopropane-1,1-dicarboxylic acid (CDA), an inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase, added to the solid culture medium of tomato nodal shoot segments resulted in a reduction in the level of endogenous ethylene according to the concentration of inhibitor applied. Following treatment with inhibitor, plants were homogenised and the concentrations of CDA and of 1-aminocyclopropane-1-carboxylic acid (ACC) were measured simultaneously in the resulting juice using an HPLC-ESI/MS-MS method. The levels of CDA and ACC measured in the plant tissues were associated with the concentration of inhibitor added to the solid medium. The HPLC-ESI/MS-MS method described produced limits of detection of 0.8 pmol for ACC and of 4 pmol for CDA.
Petritis
Konstantinos
K
Institut de Chimie Organique et Analytique, CNRS UMR-6005, Université d'Orléans, BP 6759, 45067, Orléans, Cedex 2, France. konstantinos.petritis@pnl.gov
Koukaki
Georgia
G
Koussissi
Elisabet
E
Elfakir
Claire
C
Dreux
Michel
M
Dourtoglou
Vassilis
V
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000598:Amino Acids, Cyclic / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003521:Cyclopropanes / Q000032:analysis*; D003998:Dicarboxylic Acids; D004791:Enzyme Inhibitors / Q000032:analysis; D005030:Ethylenes / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000598:Amino Acids, Cyclic; D002264:Carboxylic Acids; D003521:Cyclopropanes; D003998:Dicarboxylic Acids; D004791:Enzyme Inhibitors; D005030:Ethylenes; C419326:cyclopropane-1,1-dicarboxylic acid; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase