title: Increase of beta1-adrenergic receptor gene expression induced by nicotine in hippocampal slice of rat.
abstract: OBJECTIVE
  To investigate the effect of nicotine on beta1-adrenergic receptor (beta1-AR) in the hippocampal slice of rat.
  
  
  METHODS
  Hippocampal slices (400 microm thick) were incubated in artificial cerebrospinal fluid (ACSF) previously saturated with 95 % O2 and 5 % CO2 at 28 degree for 120 min, and then incubated with nicotine 10 micromol/L for 30, 60, 90, and 120 min. mRNA of the beta1-adrenergic receptor was examined with semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), and the protein level was measured by Western blot and RIA.
  
  
  RESULTS
  The mRNA gene expression and the protein level of beta1-adrenergic receptor in hippocampal slices were increased after nicotine treatment. The peak of protein occurred later but higher than that of mRNA level.
  
  
  CONCLUSIONS
  Both expression of beta1- adrenergic receptor gene transcription and post-transcriptional protein level in rat hippocampus were altered by nicotine.
authors.affiliation: Department of Pharmacology, Sun Yat-sen University of Medical Science, Guangzhou 510089, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Yu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Huang
authors.forename: Ru-Zhu
authors.initials: RZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018342:Receptors, Adrenergic, beta-1 / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D009538:Nicotine