Novel vanadium-binding proteins (vanabins) identified in cDNA libraries and the genome of the ascidian Ciona intestinalis.
Ascidians, especially those belonging to the suborder Phlebobranchia, can accumulate high levels of vanadium. Vanadium-binding proteins (vanabins) were first isolated from a vanadium-accumulating ascidian, Ascidia sydneiensis samea, and then the vanabins were cloned, their expression was studied, and metal-binding assays were conducted. In order to unravel the mechanism of vanadium accumulation, we searched for vanabin-like genes in other animals, including other ascidians. A database search revealed five groups of cDNAs that encoded vanabin-like proteins in another ascidian, Ciona intestinalis. The genes encoding C. intestinalis vanabins, CiVanabin1 to CiVanabin5, were clustered in an 8.4-kb genomic region. The direction of transcription of each gene was identical and each gene had a single intron. All the C. intestinalis vanabins were cysteine-rich, and the repetitive pattern of cysteines closely resembled that of A. sydneiensis samea vanabins. Using immobilized metal ion affinity chromatography (IMAC), we found that a recombinant protein of at least one of the C. intestinalis vanabins (CiVanabin5) bound to vanadium(IV) ions.
Trivedi
Subrata
S
Marine Biological Laboratory, Graduate School of Science, Hiroshima University, Japan.
Ueki
Tatsuya
T
Yamaguchi
Nobuo
N
Michibata
Hitoshi
H
D000595:Amino Acid Sequence; D000818:Animals; D002938:Ciona intestinalis / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D015723:Gene Library; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014639:Vanadium / Q000378:metabolism*
D018076:DNA, Complementary; D011506:Proteins; D014639:Vanadium