title: Microvascular basal lamina damage in thromboembolic stroke in a rat model.
abstract: We investigated microvascular damage in areas with magnetic resonance imaging (MRI)-defined apparent diffusion coefficient reduction (ADC-R) in a rat model of thromboembolic occlusion of the middle cerebral artery. Rats received either intracarotid recombinant tissue plasminogen activator (rt-PA) or saline. Microvascular basal lamina damage was quantified by immunohistochemical staining of collagen type IV and by videoimaging analysis. ADC-R positive basal ganglia (cortical) areas showed a significant reduction of stained microvascular area by 15+/-6% (8+/-7%) and the microvascular density by 13+/-5% (8+/-6%) of that on the non-ischemic control side (P<0.001). There were no significant microvascular differences between rats given rt-PA or saline, or between those with or without angiographically proven recanalization. This study reports for the first time that microvascular basal lamina damage in experimental thromboembolic stroke is confined to regions with ADC-R in MRI.
authors.affiliation: Department of Neurology, Ludwig-Maximilians University, Klinikum Grosshadern, Marchioninistrasse 15, D-81377 Munich, Germany.
authors.forename: Milan R
authors.initials: MR
authors.lastname: Vosko
authors.forename: Elmar
authors.initials: E
authors.lastname: Busch
authors.forename: Dorothe
authors.initials: D
authors.lastname: Burggraf
authors.forename: Gundula
authors.initials: G
authors.lastname: Bültemeier
authors.forename: Gerhard F
authors.initials: GF
authors.lastname: Hamann
mesh_terms: D000792:Angiography / Q000379:methods; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D001485:Basement Membrane / Q000473:pathology*; D002525:Cerebellar Cortex / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D008279:Magnetic Resonance Imaging / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000209:etiology / Q000473:pathology*; D013923:Thromboembolism / Q000209:etiology / Q000473:pathology*; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use
chemical_list: D024043:Collagen Type II; D010959:Tissue Plasminogen Activator