Ribotyping of lactobacilli isolated from spoiled beer.
Twenty-nine Lactobacillus strains contaminating beers in different Czech breweries as well as representative type strains obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and a probe made complementary to 16S and 23S rRNA genes. Biochemical test results assigned the 29 strains to the species L. brevis, L. plantarum, L. buchneri and L. paracasei subsp. paracasei. Ribotyping separated L. brevis, L. plantarum and L. paracasei subsp. paracasei strains into species-specific ribogroups in full correspondence with biotyping; L. buchneri strains were split into two ribogroups. Characteristic band patterns for each species and even typical bands of certain sizes were observed.
Yansanjav
Altanzaya
A
Department of Microbiology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
Svec
Pavel
P
Sedlácek
Ivo
I
Hollerová
Ida
I
Nemec
Miroslav
M
D001515:Beer / Q000382:microbiology*; D002965:Classification; D018153:Czech Republic; D004269:DNA, Bacterial / Q000032:analysis; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics*; D021521:Ribotyping
D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI