Alternative splicing generates a novel non-secretable resistin isoform in Wistar rats.
Resistin is a secreted adipose tissue hormone that belongs to the resistin-like molecule family. We report here a new alternatively spliced isoform of the rat resistin gene, named S-resistin (short resistin), detected in adipose tissue by reverse transcription-polymerase chain reaction (RT-PCR). A comparison of this cDNA variant and genomic sequences indicates the lack of the second exon containing the secretory consensus signal. Both cDNAs, resistin and S-resistin, were carboxy-tagged with FLAG epitope and transiently expressed in cultured cell lines. While the resistin-FLAG construct gives the expected pattern for a secretion protein, the S-resistin-FLAG construct yielded a predominant nuclear staining. These results indicate that this splicing event regulates the fate and probably the function of the mature protein.
Del Arco
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Area de Bioquímica, Centro Regional de Investigaciones Biomédicas (CRIB), Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Av De Carlos III s/n, 45071, Toledo, Spain. araceli.arco@uclm.es
Peralta
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Carrascosa
J M
JM
Ros
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M
Andrés
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Arribas
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D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006729:Hormones, Ectopic / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor; D020033:Protein Isoforms; D011506:Proteins; D051381:Rats; D017208:Rats, Wistar; D052243:Resistin; D016415:Sequence Alignment; D017385:Sequence Homology
D018076:DNA, Complementary; D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D020033:Protein Isoforms; D011506:Proteins; C499624:RETN protein, human; C486574:RETNLB protein, human; D052243:Resistin; C499623:Retn protein, mouse; C499622:Retn protein, rat; C413576:Retnla protein, mouse; C486575:Retnla protein, rat; D020932:Nerve Growth Factor