The influence of split doses of gamma-radiation on human erythrocytes.
Human erythrocyte suspensions in an isotonic Na-phosphate buffer, pH 7.4, of hematocrit of 2% were exposed under air to gamma radiation at a dose rate of 2.2 kGy. Erythrocytes were irradiated with single doses, and identical doses split into two fractions with an interval time of 3.5 h between following exposures. The obtained results indicated that the irradiation of enucleated human erythrocytes with split doses caused a reduction of hemolysis (2.4 times), a decrease in the level of damage to membrane lipids and the contents of MetHb, compared with identical single doses. However, the splitting of radiation doses did not change the level of damage to the membrane proteins, as was estimated with a maleimide spin label. The obtained results suggest that a decrease in the level of damage to lipids was related to a decrease in hemolysis.
Koziczak
Renata
R
Department of Molecular Biophysics, University of Lodz, Lodz, Poland.
Gonciarz
Marta
M
Krokosz
Anita
A
Szweda-Lewandowska
Zofia
Z
D000222:Adaptation, Physiological / Q000528:radiation effects; D002478:Cells, Cultured; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000528:radiation effects; D004912:Erythrocytes / Q000378:metabolism* / Q000528:radiation effects*; D005720:Gamma Rays; D006461:Hemolysis / Q000528:radiation effects*; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000528:radiation effects
D008563:Membrane Lipids; D008565:Membrane Proteins; D008706:Methemoglobin