title: Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation.
abstract: We investigated the effect of supplementing extended boar semen with different amounts of hyaluronan (HA) prior to freezing on post-thaw sperm characteristics. Using a split sample design, the effect of HA at a final concentration of 500 or 1000 microg/ml semen on post-thaw motility parameters, and membrane lipid architecture status assessed by merocyanine-540/YOPRO-1 and flow cytometry were evaluated. HA-supplementation improved motility parameters (P < 0.05 to P < 0.001) and decreased the percentage of hyperactivated spermatozoa (P < 0.05). HA-supplemented samples had more spermatozoa showing high lipid membrane stability as assessed with merocyanine-540. In conclusion, HA appeared to preserve post-thaw spermatozoa viability in vitro and maintained membrane stability after cryopreservation.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences (SLU), SE-750-07 Uppsala, Sweden. fjuanpvega@unex.es
authors.forename: F J
authors.initials: FJ
authors.lastname: Peña
authors.forename: A
authors.initials: A
authors.lastname: Johannisson
authors.forename: M
authors.initials: M
authors.lastname: Wallgren
authors.forename: H
authors.initials: H
authors.lastname: Rodriguez-Martinez
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008055:Lipids / Q000032:analysis*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine
chemical_list: D008055:Lipids; D006820:Hyaluronic Acid