New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.
Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by the new RT-PCR test (52 versus 76 versus 80%; P = 0.003). This new reliable EV RNA detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurological syndromes.
Jacques
Jérôme
J
Laboratoire de Virologie, Centre Hospitalier Universitaire, IFR 53/EA-3309 Faculté de Médecine de Reims, Reims, France.
Carquin
Jocelyne
J
Brodard
Véronique
V
Moret
Hélène
H
Lebrun
Dorine
D
Bouscambert
Maude
M
Motte
Jacques
J
Rémy
Gérard
G
Andréoletti
Laurent
L
D002555:Cerebrospinal Fluid / Q000821:virology; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D006801:Humans; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000821:virology*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity