Isolation and characterisation of proline/arginine-rich cathelicidin peptides from ovine neutrophils.
Cathelicidins are a family of gene-encoded antimicrobial peptides found in mammals. Seven cathelicidin genes have been identified in sheep, but up to now only two variants of one of these predicted peptides (OaBac5) have been purified from ovine neutrophils. In this work numerous proline/arginine-rich cathelicidin peptides were purified, including the originally predicted OaBac5 and another OaBac5 variant. As well as this, the C-terminus of the predicted OaBac7.5 and various truncated forms of OaBac11 were purified. Even though these peptides were much smaller than those predicted, they still displayed antimicrobial activity.
Anderson
Rachel C
RC
Biotechnology Group, Institute of Technology and Engineering, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
Yu
Pak-Lam
PL
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001120:Arginine; D001798:Blood Proteins; D002176:Candida albicans / Q000187:drug effects; D002417:Cattle; D004926:Escherichia coli / Q000187:drug effects; D006041:Goats; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010456:Peptides, Cyclic; D011392:Proline; D017385:Sequence Homology; D012756:Sheep; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects; D023084:alpha-Defensins / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D010456:Peptides, Cyclic; D023084:alpha-Defensins; C056454:bactenecin; D001120:Arginine; D011392:Proline