title: Immature granulocyte detection by the SE-9000 haematology analyser during pregnancy.
abstract: The objective of this study was to determine the nature of the alarm for immature granulocytes appearing in haemograms from pregnant women, as detected by the immature cell information channel (IMI) of the SE-9000 automated haematology analyser. Of all tests run on pregnant women in a 4-month period (n = 698), the first 100 haemograms with immature granulocyte alarms (14.33%) were collected. Each of these samples was then stained with Wright-Giemsa stain. The following variables were also analysed: age of the mother, trimester and days of gestation, type of delivery, weight and sex of the baby, and Apgar score. Most pregnant women were in the third trimester of gestation (82%) when an alarm was noted on the IMI channel. Of the patients, 62% had normal deliveries. The most frequent complication was obstructed delivery (23%). Mean percentages by microscopic counts of band cells, metamyelocytes, and myelocytes were 2.99, 0.45, and 0.19%, respectively. There was a statistically significant correlation for all cell types between the SE-9000 and the manual count method. No association was observed between the presence of immature granulocytes and the clinical variables analysed. The SE-9000 analyser shows high sensitivity in the IMI channel for detection of immature forms.
authors.affiliation: Department of Immunology, Virgen del Rocío University Hospitals, Seville, Spain. afernandezsuarez@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Suárez
authors.forename: V T
authors.initials: VT
authors.lastname: Pascual
authors.forename: M T F
authors.initials: MT
authors.lastname: Gimenez
authors.forename: J F S
authors.initials: JF
authors.lastname: Hernández
mesh_terms: D000328:Adult; D001331:Automation; D001724:Birth Weight; D002454:Cell Differentiation; D036861:Delivery, Obstetric; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007564:Japan; D007958:Leukocyte Count / Q000295:instrumentation*; D007964:Leukocytosis / Q000097:blood; D008423:Maternal Age; D011247:Pregnancy / Q000097:blood*; D011263:Pregnancy Trimester, Third; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013194:Staining and Labeling