Dendritic molecular capsules for hydrophobic compounds.
Reichardt's dye, a highly solvatochromic dye, was encapsulated within poly (glycerol succinic acid) ([Gn]-PGLSA-OH) dendrimers to investigate the interior environment of these dendritic macromolecules. The absorption maximum for the encapsulated Reichardt's dye in water was indicative of a relatively high dielectric constant present within the dye/dendrimer complex. (1)H NMR of the encapsulated complex showed the presence of aromatic protons from Reichardt's dye along with the aliphatic protons of the dendrimer. Additionally, there were substantial changes in T(1) and T(2) times of the encapsulated dye when compared with the free dye, and (1)H NOESY spectra for the complex showed a significant number of intermolecular NOE cross-peaks. These data reveal the close through-space proximity of the dye to the dendrimer and the restricted motion of the encapsulated dye. To demonstrate the potential use of these macromolecules as drug delivery vehicles, the poorly water-soluble anticancer drug 10-hydroxycamptothecin (10HCPT) was encapsulated within a carboxylated PGLSA dendrimer ([G4]-PGLSA-COONa). Cytotoxicity assays with human breast cancer cells showed a significant reduction of cell viability, demonstrating that 10HCPT retains activity upon encapsulation.
Morgan
Meredith T
MT
Departments of Chemistry and Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Carnahan
Michael A
MA
Immoos
Chad E
CE
Ribeiro
Anthony A
AA
Finkelstein
Stella
S
Lee
Stephen J
SJ
Grinstaff
Mark W
MW
D001555:Benzene Derivatives / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013386:Succinates / Q000737:chemistry*
D001555:Benzene Derivatives; D004396:Coloring Agents; D011108:Polymers; D013386:Succinates; C043941:polyglycerol; D005990:Glycerol