title: [Pollution of EHEC O157:H7 in six types of food in Henan Province].
abstract: In order to investigate the distribution of contamination of EHEC O157:H7 in 6 types of food in Henan Province and characterize the effect of seasonal factors on distribution of O157:H7 contamination so as to prevent and control food contamination, Henan Province was divided into five sampling areas according to the natural geographical features. Samples were taken randomly in summer and winter sales links. After selective culture of increasing reproduction of O157:H7, it was screened by immuno-gold reagent, and pathogenic bacterium were isolated after accumulation of immunomagnetic. Then, it was identified by the bioMérieux VITEK32 AMS system, GNI+ and sero-reaction. Results showed that 28 EHEC O157:H7 were isolated in 1463 samples among which the positive rate of raw meat and fresh vegetable was 3.3% and 3.2% respectively (the highest of all), and EHEC O157:H7 was not found in yogurt. The positive rate in summer (2.5%) was obviously higher than that in winter (1.1%). It could be concluded that the contamination of EHEC O157:H7 in food was serious. Its positive rate was positively correlated with the epidemic of infective diarrhea. Therefore, attention should be given by relevant departments concerned and spervision and testing should be strengthened in livestock and farmyard bird slaughtering link, vegetable production link and sales link for better prevention of the breaking out and epidemic of food born diseases.
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou 450003, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhang
authors.forename: Xingguang
authors.initials: X
authors.lastname: Liao
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002681:China; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D018189:Immunomagnetic Separation; D008460:Meat / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology; D015014:Yogurt / Q000382:microbiology