Endotoxin-induced changes in human working and declarative memory associate with cleavage of plasma "readthrough" acetylcholinesterase.
Endotoxin stimulation of the immune system produces marked alterations in memory functioning. However, molecular links between this cognitive response and infection-responding neurotransmission pathways are still unknown. The cytokine and memory responses of volunteers injected with 0.8 ng/kg Salmonella endotoxin were compared with changes in plasma levels and integrity of the stress-induced acetylcholinesterase variant, AChE-R. Vascular endothelial cells were found to express AChE-R messenger RNA and protein both in healthy and inflamed human tissues. Plasma AChE activity was reduced after endotoxin treatment, but not placebo treatment, parallel to the decline in cortisol after the endotoxin-induced peak and inversely to the accumulation of a C-terminal immunopositive AChE-R peptide of 36 amino acid residues. AChE-R cleavage coincided with significant endotoxin-induced improvement in working memory and impairment in declarative memory. By 3 h posttreatment, working memory improvement was negatively correlated with AChE-R cleavage, which showed association to proinflammatory cytokine levels. By 9 h posttreatment, declarative memory impairment was negatively correlated with AChE-R cleavage and positively correlated with the suppressed AChE activity. Endotoxin-induced peripheral cholinergic stress responses are hence associated with greater impairment in declarative memory and lower improvement in working memory, pointing at AChE-R as a surrogate marker of psychoneuroimmunological stress.
Cohen
Osnat
O
Department of Biological Chemistry and The Eric Roland Center for Neurodegenerative Diseases, The Hebrew University of Jerusalem, Israel 91904.
Reichenberg
Abraham
A
Perry
Chava
C
Ginzberg
Dalia
D
Pollmächer
Thomas
T
Soreq
Hermona
H
Yirmiya
Raz
R
D000110:Acetylcholinesterase / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000097:blood; D004731:Endotoxins / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D008569:Memory Disorders / Q000097:blood / Q000139:chemically induced / Q000201:enzymology*; D015213:Neuroimmunomodulation / Q000187:drug effects* / Q000276:immunology; D010446:Peptide Fragments / Q000097:blood; D012333:RNA, Messenger / Q000097:blood; D011930:Reaction Time / Q000187:drug effects / Q000276:immunology; D013312:Stress, Physiological / Q000097:blood / Q000139:chemically induced / Q000201:enzymology
D015415:Biomarkers; D016207:Cytokines; D004731:Endotoxins; D010446:Peptide Fragments; D012333:RNA, Messenger; D000110:Acetylcholinesterase; D006854:Hydrocortisone