title: Localization of mechanosensitive channel TRPV4 in mouse skin.
abstract: A transient receptor potential (TRP) family, TRPV4, is a calcium-permeable swell-activated channel, playing a role in cutaneous mechanosensation. To elucidate the localization in the mechanosensitive endings, we found with immunohistochemistry in mice that TRPV4 was expressed both by small (low threshold) and large (high threshold) dorsal root ganglia neurons. In addition to free nerve endings, TRPV4 was specifically located at cutaneous mechanosensory terminals co-localized with neurofilament 200, including Meissner, Merkel, penicillate and intraepidermal terminals but not including hair follicle palisades. The distribution suggests that the sensation of pressure by mechanosensitive TRPV4 channel is transmitted through A- as well as C-fiber.
authors.affiliation: Department of Pharmacology, Jichi Medical School, Kawachi, Tochigi 329-0498, Japan. macsuz@jichi.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yuki
authors.initials: Y
authors.lastname: Oyama
authors.forename: Atsuko
authors.initials: A
authors.lastname: Mizuno
authors.forename: Eiji
authors.initials: E
authors.lastname: Kusano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirao
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ookawara
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D008465:Mechanoreceptors / Q000378:metabolism; D008562:Membrane Glycoproteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D064531:Peripherins; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D050916:TRPV Cation Channels
chemical_list: D027682:Cation Transport Proteins; D007381:Intermediate Filament Proteins; D007473:Ion Channels; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D064531:Peripherins; D050916:TRPV Cation Channels; C486576:Trpv4 protein, mouse; C051774:neurofilament protein H