Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis.
Four aryl-phospho-beta- d-glucosidases were identified in Bacillus subtilis by using 4-methylumbelliferyl-phospho-beta- d-glucopyranoside as a substrate. Two of these enzymes are the products of the bglA and bglH genes, previously suggested to encode aryl-phospho-beta- d-glucosidases, while the other enzymes are encoded by the yckE and ydhP genes. Together, these four genes account for >99.9% of the glucosidase activity in B. subtilis on aryl-phospho-beta- d-glucosides. yckE was expressed at a low and constant level during growth, sporulation, and spore germination, and was not induced by aryl-beta- d-glucosides. ydhP was also not induced by aryl-beta- d-glucosides. However, while ydhP was expressed at only a very low level in exponential-phase cells and germinating spores, this gene was expressed at a higher levels upon entry into the stationary phase of growth. Strains lacking yckE or ydhP exhibited no defects in growth, sporulation, or spore germination or in growth on aryl-beta- d-glucosides. However, a strain lacking bglA, bglH and yckE grew poorly if at all on aryl-beta- d-glucosides as the sole carbon source.
Setlow
Barbara
B
Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA.
Cabrera-Hernandez
Arturo
A
Cabrera-Martinez
Rosa Martha
RM
Setlow
Peter
P
D001104:Arbutin / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001592:Benzyl Alcohols / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005960:Glucosides; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism
D001592:Benzyl Alcohols; D005960:Glucosides; D006923:Hymecromone; C005696:salicin; D001104:Arbutin; D005959:Glucosidases; D005947:Glucose