title: Crystallization of CprB, an autoregulator-receptor protein from Streptomyces coelicolor A3(2).
abstract: CprB, an autoregulator-receptor protein from Streptomyces coelicolor A3(2), was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 6000 as a precipitant. Three crystal forms (I, II and III) were obtained; crystal forms I and II were useful for structure determination. Form I crystals belong to an orthorhombic system with space group P2(1)2(1)2(1) and diffracted to better than 2.4 A. Form II crystals belong to a tetragonal system with space group P4(1(3))2(1)2.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. matsume@jbirc.go.jp
authors.forename: Ryo
authors.initials: R
authors.lastname: Natsume
authors.forename: Ryo
authors.initials: R
authors.lastname: Takeshita
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics; D013302:Streptomyces / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D012097:Repressor Proteins