title: Design and fabrication of standardized hydroxyapatite scaffolds with a defined macro-architecture by rapid prototyping for bone-tissue-engineering research.
abstract: This investigation describes the production and characterization of calcium phosphate scaffolds with defined and reproducible porous macro-architectures and their preliminary in vitro and in vivo bone-tissue-engineered response. Fugitive wax molds were designed and produced using a rapid prototyping technique. An aqueous hydroxyapatite slurry was cast in these molds. After sintering at 1250 degrees C and then cleaning, dimensional and material characterizations of the scaffolds were performed. The resulting scaffolds represented the design, and their dimensions were remarkably consistent. A texture inherent to the layer-by-layer production of the mold was impressed onto the vertical surfaces of the scaffolds. The surface roughness (R(a)) of the textured surfaces was significantly greater than that of the nontextured surfaces. Material analyses revealed a beta-TCP phase in addition to hydroxyapatite for the molded ceramics. Non-molded control ceramics exhibited only hydroxyapatite. Thirty scaffolds were seeded with culture-expanded goat bone-marrow stromal cells (BMSCs) and implanted subcutaneously in nude mice for 4 or 6 weeks. Histology revealed mineralized bone formation in all the scaffolds for both implantation periods. After 4 weeks, bone was present primarily as a layer on scaffold surfaces. After 6 weeks, the surface bone formation was accompanied by bone budding from the surface and occasional bridging of pores. This budding and bridging bone formation almost always was associated with textured scaffold surfaces. However, the area percentage of bone in pores was similar for the 4- and 6-week implantation periods.
authors.affiliation: Department of Orthopaedics, University Medical Center, PO Box 85500, 3508 GA Utrecht, The Netherlands. c.e.wilson@chir.azu.nl
authors.forename: C E
authors.initials: CE
authors.lastname: Wilson
authors.forename: J D
authors.initials: JD
authors.lastname: de Bruijn
authors.forename: C A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.forename: A J
authors.initials: AJ
authors.lastname: Verbout
authors.forename: W J A
authors.initials: WJ
authors.lastname: Dhert
mesh_terms: D000818:Animals; D001846:Bone Development / Q000502:physiology; D018786:Bone Substitutes / Q000138:chemical synthesis*; D001842:Bone and Bones; D002516:Ceramics; D015195:Drug Design; D017886:Durapatite / Q000138:chemical synthesis* / Q000737:chemistry*; D006041:Goats; D006801:Humans; D007085:Ilium / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D023822:Tissue Engineering / Q000379:methods
chemical_list: D018786:Bone Substitutes; D017886:Durapatite