Antioxidant properties of dipyridamole as assessed by chemiluminescence.
The ability of dipyridamole (DIP) to scavenge oxygen metabolites generated by either activated human neutrophils (PMNs) or cell-free systems using luminol(s)- and lucigenin-enhanced chemiluminescence was investigated. In the presence of DIP (15-50 microM) a dose-dependent inhibition period was seen in phorbol myristate acetate (PMA)-stimulated PMNs as assayed by isoluminol-enhanced chemiluminescence (ILCL) with horseradish peroxidase (HRP). Although such a lag period was not observed in the absence of HRP, 50 microM DIP inhibited extracellular ILCL by more than 50%. Intracellular luminol-enhanced chemiluminescence (LCL) as assayed in either PMA- or in ionomycin-activated PMNs was not affected by dipyridamole (15-50 microM). In cell-free systems, DIP produced concentration-dependent inhibition in H2O2-(45% at 50 microM), OH- (40%, at 0.1 microM) and HOCl-(20% at 10 microM). Both absorbance and fluorescence scans revealed that DIP is able to react with equimolar quantities of either H202 or HOCl. These results suggest that DIP scavenges reactive oxygen species (ROS) presumably secreted by activated human PMNs in the following decreasing order: *OH > HOCl > H2O2 >> O2-.
Vargas
F
F
Laboratorio de Fotoquímica, Centro de Química, Institute Venezolano de Insvestigaciones Científicas I.V.I.C., Caracas, Venezuela. fvargas@ivic.ivic.ve
Rivas
C
C
Díaz
Y
Y
Contreras
N
N
Silva
A
A
Ojeda
L E
LE
Velásquez
M
M
Fraile
G
G
D000166:Acridines / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D045304:Cytochromes c / Q000378:metabolism; D004176:Dipyridamole / Q000737:chemistry*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry
D000166:Acridines; D000975:Antioxidants; D016877:Oxidants; D017382:Reactive Oxygen Species; C033472:10,10'-dimethyl-9,9'-biacridinium; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004176:Dipyridamole; D006997:Hypochlorous Acid; D045304:Cytochromes c; D006861:Hydrogen Peroxide