[The clinical significance of the expression of four cell cycle regulators in breast papillomotosis].
OBJECTIVE
To investigate the clinical significance of the protein expression of 4 cell cycle regulators in papillomatosis of the breast.


METHODS
Immunohistochemistry was used to examine the protein expression of CyclinD1, p16, E2F-1 and Rb in 4 groups (196 cases) with mild papillomatosis (MP), moderate papillomatosis (MoP), serious papillomatosis (SP) and ductal carcinoma in situ (DCIS).


RESULTS
There were significant difference of the positive rate and the intensity of CyclinD1, p16, E2F-1 and Rb expression among the groups, respectively (chi(2) were 16.702, 20.742, 40.335, 42.317; 19.120, 29.469, 45.080, 46.920, P < 0.01. Meaning time there were significant difference in the expression of these 4 factors between MoP and SP (P < 0.05). There was significance of E2F-1 or Rb expression between SP and DCIS (P < 0.01), but there was no significance to be found in the expression of CyclinD1 or p16 between the two groups (P > 0.05). Rb was screened as the highest risk factor by Odd Risk Logistic Analysis.


CONCLUSIONS
CyclinD1, p16, E2F-1 and Rb played the important roles from MP into SP and then DCIS. E2F-1 and Rb can be used as the assistant indicators on the differentiation of SP and DCIS. Rb may be helpful in screening high risk cases from SP or MoP.
Niu
Yun
Y
Tianjin Cancer Institute and Cancer Hospital, Tianjin Medical University, Tianjin 300060, China.
Liu
Tie-ju
TJ
Li
Yu
Y
Yu
Yong
Y
Lü
A-juan
AJ
Fu
Xi-lin
XL
D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D019938:Cyclin D1 / Q000032:analysis; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D004268:DNA-Binding Proteins; D003937:Diagnosis, Differential; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D010212:Papilloma / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D016160:Retinoblastoma Protein / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis
D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D016160:Retinoblastoma Protein; D014157:Transcription Factors; D019938:Cyclin D1