[Primary research of the effect of intestinal ischemia/reperfusion injury on Leptin concentrations].
OBJECTIVE
To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses.


METHODS
An intestinal ischemia-reperfusion injury model of rats was established, and used a highly-sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue.


RESULTS
Serum Leptin level (10.82+/-0.83) microg/L significantly decreased after an injury of 60-minute ischemia and 30-minute reperfusion versus pre-experimental serum levels (16.46+/-3.21) microg/L; Leptin level in serum was higher than that in adipose tissue (4.466+/-2.63) mg/100g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended (P=0.047).


CONCLUSIONS
Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/reperfusion.
Lin
Ji
J
Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of People's Liberation Army, Beijing 100853, China.
Yan
Guang-tao
GT
Hao
Xiu-hua
XH
Zhang
Kai
K
Wang
Lu-huan
LH
Xue
Hui
H
D000818:Animals; D005260:Female; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*
D020738:Leptin