[Application of precision-cut liver slice to the study of the effect of glutathione on cadmium chloride hepatotoxicity].
In order to evaluate the feasibility of application of precision cut liver slice(PCLS) to the study of the effect of glutathione on cadmium chloride hepatotoxicity, slices were cultured with CdCl2 and GSH. Slices activity and drug metabolic function was estimated by assay of slice viability, NO secretion, and phase I and phase II enzyme activity. Results showed that the slices activity was significantly inhibited by CdCl2. Various phase I and phase II enzymes activity of slices were obviously reduced by CdCl2, but aniline hydroxidase activity was enhanced. GSH could distinctly reversed the change of slices induced by CdCl2. Therefore, it could be concluded that PCLS culture system was sensitive and steady, and was a good model for pharmacological and toxicological investigation of liver in vitro.
Yang
Zheqiong
Z
Department of Pharmacology, Medical College, Wuhan University, Wuhan 430071, China.
Peng
Renxiu
R
Xi
Jinlei
J
Wu
Junyu
J
D000818:Animals; D019256:Cadmium Chloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D046508:Culture Techniques; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D005978:Glutathione; D019256:Cadmium Chloride