title: Lipid dynamics in yeast under haem-induced unsaturated fatty acid and/or sterol depletion.
abstract: In the yeast Saccharomyces cerevisiae, UFA (unsaturated fatty acids) and ergosterol syntheses are aerobic processes that require haem. We took advantage of a strain affected in haem synthesis ( hem1 Delta) to starve specifically for one or the other of these essential lipids in order to examine the consequences on the overall lipid composition. Our results demonstrate that reserve lipids (i.e. triacylglycerols and steryl esters) are depleted independently of haem availability and that their UFA and sterol content is not crucial to sustain residual growth under lipid depletion. In parallel to UFA starvation, a net accumulation of SFA (saturated fatty acids) is observed as a consequence of haem biosynthesis preclusion. Interestingly, the excess SFA are not mainly stored within triacylglycerols and steryl esters but rather within specific phospholipid species, with a marked preference for PtdIns. This results in an increase in the cellular PtdIns content. However, neutral lipid homoeostasis is perturbed under haem starvation. The contribution of two lipid particle-associated proteins (namely Tgl1p and Dga1p) to this process is described.
authors.affiliation: Laboratoire de Génétique de la Levure, CNRS-UMR6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers CEDEX, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Ferreira
authors.forename: Matthieu
authors.initials: M
authors.lastname: Régnacq
authors.forename: Parissa
authors.initials: P
authors.lastname: Alimardani
authors.forename: Carole
authors.initials: C
authors.lastname: Moreau-Vauzelle
authors.forename: Thierry
authors.initials: T
authors.lastname: Bergès
mesh_terms: D000217:Acyltransferases / Q000502:physiology; D051048:Diacylglycerol O-Acyltransferase; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000096:biosynthesis*; D017353:Gene Deletion; D006418:Heme / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D010716:Phosphatidylinositols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013261:Sterols / Q000096:biosynthesis* / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010716:Phosphatidylinositols; D010743:Phospholipids; D013261:Sterols; D014280:Triglycerides; D006418:Heme; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase