Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMPhi cells from rats.
OBJECTIVE
To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.


METHODS
With carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.


RESULTS
Decoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.


CONCLUSIONS
Decoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats.
Guo
Hua
H
Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Liang
Hua-ping
HP
Lu
Feng-lin
FL
Xu
Xiang
X
An
Bin
B
Hao
Tian-zhi
TZ
Wang
Fu-lLong
FL
Yang
Wen-jun
WJ
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10