title: The crystal structure of murine p97/VCP at 3.6A.
abstract: p97/VCP is a member of the AAA ATPase family and has roles in both membrane fusion and ubiquitin dependent protein degradation. Here, we present a 3.6A crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent. The resulting structure illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement observed in this structure is in contrast to our previously predicted tail-to-tail packing model. The linker between the D1 and D2 domains is partially disordered, suggesting a flexible nature. Normal mode analysis of the crystal structure suggests anti-correlated motions and distinct conformational states of the two AAA domains.
authors.affiliation: Department of Biological Sciences, Imperial College London, South Kensington SW7 2AZ, UK.
authors.forename: Trevor
authors.initials: T
authors.lastname: Huyton
authors.forename: Valerie E
authors.initials: VE
authors.lastname: Pye
authors.forename: Louise C
authors.initials: LC
authors.lastname: Briggs
authors.forename: Terence C
authors.initials: TC
authors.lastname: Flynn
authors.forename: Fabienne
authors.initials: F
authors.lastname: Beuron
authors.forename: Hisao
authors.initials: H
authors.lastname: Kondo
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Ma
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Paul S
authors.initials: PS
authors.lastname: Freemont
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry; D000818:Animals; D016880:Anisotropy; D018797:Cell Cycle Proteins / Q000737:chemistry*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004583:Electrons; D049071:Endopeptidase Clp; D051379:Mice; D008958:Models, Molecular; D015233:Models, Statistical; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012697:Serine Endopeptidases / Q000737:chemistry; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein; C000614179:Vcp protein, mouse