title: Altered action of dopamine and cholecystokinin on lateral hypothalamic neurons in rats raised under different feeding conditions.
abstract: Single-unit activity was recorded in the lateral hypothalamus (LH) of adult Wistar rats anaesthetized with urethane. The rats were differently nourished till weaning by raising in small (SL), control (CL) or large litters (LL). They gained significantly different body weight leading to overweight in SL (mean: 428.4 g on day 90) and underweight in LL rats (mean 399.5 g) compared to CLs (414.5 g). The mean basal firing rate of LH neurons differed, it was lowest in SL and highest in LL rats. The proportion of neurons changing their firing rate by more than 30% in response to iontophoretically administered dopamine (DA) was significantly greater in SL (76%) than LL rats (54%). Effects of DA were significantly more often blocked by a D1 receptor antagonist in LL than CLs. The responsiveness to cholecystokinin (CCK) alone and coadministered with DA was also greater in SL than LL. Furthermore, the proportion of neurons inhibited by DA alone and in the presence of CCK was significantly greater in SL than LL rats. In conclusion, litter size and difference in nourishment during early postnatal development of rats seem to determine LH basal firing rate. The increased neuronal responsiveness to exogenous DA and CCK in neonatally overfed SL rats may indicate a decreased activity of these endogenous signals which normally contribute to limitation of energy intake.
authors.affiliation: Johannes Mueller Institute of Physiology, Charité, Humboldt University Berlin, Tucholskystr. 2, D-10117 Berlin, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Zippel
authors.forename: Andreas
authors.initials: A
authors.lastname: Plagemann
authors.forename: Helga
authors.initials: H
authors.lastname: Davidowa
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D001552:Benzazepines / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D022062:Electrophysiologic Techniques, Cardiac; D005247:Feeding Behavior; D007031:Hypothalamus / Q000166:cytology*; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008536:Meglumine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D018697:Nootropic Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D012457:Salicylamides / Q000494:pharmacology; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C090019:8-sulfocholecystokinin octapeptide; D001552:Benzazepines; D018492:Dopamine Antagonists; D007211:Indoles; D018697:Nootropic Agents; C102662:PD 140548; D011949:Receptors, Cholecystokinin; D012457:Salicylamides; C065600:PD 135158; D008536:Meglumine; D002766:Cholecystokinin; C045989:eticlopride; D012844:Sincalide; D004298:Dopamine