Oligosaccharide identification and mixture quantification using Raman spectroscopy and chemometric analysis.
This work demonstrates the feasibility of using Raman spectroscopy for the analysis of small quantities of chemically similar oligosaccharides and their mixtures. Raman spectra were obtained from 10-microL aliquots of 1 mM solutions of maltotetraose and/or stachyose after deposition onto an electrochemically roughened silver substrate (and the resulting spectral features are attributed to a combination of normal and surface-enhanced Raman scattering). These compounds were selected because they are representative of glycans derived from post-translationally modified proteins which, like these compounds, often consist of isomers of equal mass and similar shape. Replicate spectral measurements were recorded and processed using a partial-least-squares (PLS) classification and quantification algorithms with a leave-one-batch-out (LOBO) training and testing procedure. Spectra derived from solutions of individual sugars were identified with 100% accuracy, and mixtures of the two sugars were quantified with an average error of 2.7% in the relative maltotetraose/stachyose composition for mixtures with a total oligosaccharide concentration of 1 mM.
Mrozek
Melissa F
MF
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Zhang
Dongmao
D
Ben-Amotz
Dor
D
D000465:Algorithms; D007536:Isomerism; D008320:Maltose / Q000031:analogs & derivatives / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods
D009844:Oligosaccharides; D011134:Polysaccharides; D011506:Proteins; C005695:stachyose; D008320:Maltose; C009819:maltotetraose