title: Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues.
abstract: The Sec machinery is one mechanism used by bacteria to translocate proteins across their cytoplasmic membrane. Most of the Sec components have been identified within the important gastric pathogen, Helicobacter pylori, however their functionality has not yet been demonstrated. Here we report the existence of putative homologues to the Sec components yajC (HP1450) and yidC (HP1551), and demonstrate the ability of the H. pylori secD (HP1550) and secG (HP1255) homologues to facilitate inner membrane translocation of the maltose-binding protein MalE, by complementation of the respective secretion-deficient Escherichia coli mutants, thus providing evidence of their functionality.
authors.affiliation: Institute of Infection, Immunity, and Inflammation, University of Nottingham, Queen's Medical School, C-floor West block, Nottingham NG7 2UH, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Fitchen
authors.forename: Paul
authors.initials: P
authors.lastname: Williams
authors.forename: Kim R
authors.initials: KR
authors.lastname: Hardie
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D016480:Helicobacter pylori / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D000069816:SEC Translocation Channels
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C499394:SecD protein, E coli; C088616:SecG protein, E coli; C412443:YIDC protein, E coli; C116101:YajC protein, Brucella; C075767:secD protein, Bacteria