Structures, locations, and transfer frequencies of genetic elements conferring high-level gentamicin resistance in Enterococcus faecalis isolates in Greece.
The elements conferring high-level gentamicin resistance in 64 clinical isolates of Enterococcus faecalis were characterized by PCR and by restriction enzyme hybridization analysis of genomic and plasmid DNA. There was a strong association between gentamicin resistance and the aac(6')-aph(2") gene carried on IS256-based elements with different structures, locations, and transfer characteristics.
Daikos
George L
GL
First Department of Propaedeutic Medicine, Athens University, Athens, Greece. gdaikos@med.uoa.gr
Bamias
George
G
Kattamis
Christos
C
Zervos
Marcus J
MJ
Chow
Joseph W
JW
Christakis
George
G
Petrikkos
George
G
Triantafyllopoulou
Penelope
P
Alexandrou
Helen
H
Syriopoulou
Vassiliki
V
D000123:Acetyltransferases / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D005839:Gentamicins / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006115:Greece; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005839:Gentamicins; C108453:6'-aminoglycoside acetyltransferase-2''-aminoglycoside phosphotransferase; D000123:Acetyltransferases; D017853:Phosphotransferases (Alcohol Group Acceptor)