title: Examination of optical depth effects on fluorescence imaging of cardiac propagation.
abstract: Optical mapping with voltage-sensitive dyes provides a high-resolution technique to observe cardiac electrodynamic behavior. Although most studies assume that the fluorescent signal is emitted from the surface layer of cells, the effects of signal attenuation with depth on signal interpretation are still unclear. This simulation study examines the effects of a depth-weighted signal on epicardial activation patterns and filament localization. We simulated filament behavior using a detailed cardiac model, and compared the signal obtained from the top (epicardial) layer of the spatial domain with the calculated weighted signal. General observations included a prolongation of the action upstroke duration, early upstroke initiation, and reduction in signal amplitude in the weighted signal. A shallow filament was found to produce a dual-humped action potential morphology consistent with previously reported observations. Simulated scroll wave breakup exhibited effects such as the false appearance of graded potentials, apparent supramaximal conduction velocities, and a spatially blurred signal with the local amplitude dependent upon the immediate subepicardial activity; the combination of these effects produced a corresponding change in the accuracy of filament localization. Our results indicate that the depth-dependent optical signal has significant consequences on the interpretation of epicardial activation dynamics.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA. mark.bray@vanderbilt.edu
authors.forename: Mark-Anthony
authors.initials: MA
authors.lastname: Bray
authors.forename: John P
authors.initials: JP
authors.lastname: Wikswo
mesh_terms: D000200:Action Potentials; D000465:Algorithms; D002455:Cell Division; D004396:Coloring Agents / Q000494:pharmacology; D006321:Heart / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008955:Models, Cardiovascular; D015233:Models, Statistical; D009206:Myocardium / Q000473:pathology*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D004396:Coloring Agents