Protein oxidation at the air-lung interface.
Whilst performing its normal functions the lung is required to deal with a range of toxic insults. Whether these are infectious agents, allergens or air pollutants they subject the lung to a range of direct and indirect oxidative stresses. In many instances these challenges lead to oxidative alterations of peptides and proteins within the lung. Measurement of protein oxidation products permits the degree of oxidative stress to be assessed and indicates that endogenous antioxidant defences are overwhelmed. The range of protein oxidation products observed is diverse and the nature and extent of specific oxidation products may inform us about the nature of the damaging ROS and NOS. Recently, there has been a significant shift away from the measurement of these oxidation products simply to establish the presence of oxidative stress, to a focus on identifying specific proteins sensitive to oxidation and establishing the functional consequences of these modifications. In addition the identification of specific enzyme systems to repair these oxidative modifications has lead to the belief that protein function may be regulated through these oxidation reactions. In this review we focus primarily on the soluble protein components of within the surface liquid layer in the lung and the consequence of their undue oxidation.
Kelly
F J
FJ
Air Pollution and Health Research Group, School of Health & Life Sciences, King's College London, London, United Kingdom. frank.kelly@kcl.ac.uk
Mudway
I S
IS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009585:Nitrogen Dioxide / Q000633:toxicity; D015538:Nitrosation; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010126:Ozone / Q000633:toxicity; D011506:Proteins / Q000187:drug effects / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000975:Antioxidants; D011506:Proteins; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D010126:Ozone; D008715:Methionine; D003545:Cysteine; D009585:Nitrogen Dioxide