Comparative sequence analysis of the tuf and recA genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating Bifidobacterium lactis from Bifidobacterium animalis.
The relationship between Bifidobacterium lactis and Bifidobacterium animalis was examined by comparative analysis of tuf and recA gene sequences and by restriction fragment length polymorphism analysis of their internal 16S-23S transcribed spacer region sequences. The bifidobacterial strains investigated could be divided into two distinct groups within a single species based on the tuf, recA, and 16S-23S spacer region sequence analysis. Therefore, all strains of B. lactis and B. animalis could be unified as the species B. animalis and divided into two subspecies, Bifidobacterium animalis subsp. lactis and Bifidobacterium animalis subsp. animalis.
Ventura
Marco
M
Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland. m.ventura@ucc.ie
Zink
Ralf
R
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010444:Peptide Elongation Factor Tu / Q000737:chemistry* / Q000235:genetics; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D051381:Rats; D011938:Rec A Recombinases / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011938:Rec A Recombinases; D010444:Peptide Elongation Factor Tu