title: Molecular genetic diversity of the French-American grapevine hybrids cultivated in North America.
abstract: French-American hybrid grapevines are most popular in eastern and mid-western North America: they are hardy cultivars derived from crosses between the European Vitis vinifera and American wild vines. The aim of this study was to characterize their genetic background using 6 microsatellite (SSR) markers and a set of 33 diagnostic RAPD markers. The latter were reproducible with different PCR thermal cyclers. Two SSR loci were found to be synonymous, VrZAG47 and VVMD27. The DNA profile frequencies estimated for each cultivar were much lower with multi-locus SSR data than that obtained from multi-fragment RAPD data. There was no significant correlation between the multi-locus DNA profile frequencies derived from SSRs and those from RAPDs. Estimates of genetic diversity derived from SSRs were generally higher and the average similarity between cultivars was generally lower than values reported for subgroups of V. vinifera, in accordance with expectations for hybrid cultivars. The phenetic relationships depicted by UPGMA (unweighted pair-group method with arithmetic averaging) and neighbor-joining analyses of microsatellite data were congruent and, to a large extent, in agreement with the known pedigree or history of each cultivar. A major dichotomy was observed between one group where the known genetic background was dominated by the North American Vitis riparia and Vitis labrusca, and another one where the genetic background was dominated by the European V. vinifera. Two Kulhmann varieties thought to be synonymous were found to be different, though closely related.
authors.affiliation: Chaire de recherche du Canada en génomique forestière et environnementale, Centre de recherche en biologie forestière, Université Laval, Sainte-Foy, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Pollefeys
authors.forename: Jean
authors.initials: J
authors.lastname: Bousquet
mesh_terms: D000465:Algorithms; D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D009656:North America; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D027843:Vitis / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant