title: Isolation of lung tumor specific peptides from a random peptide library: generation of diagnostic and cell-targeting reagents.
abstract: Discovery of ligands specific to receptor(s) on a surface of a cancer cell could impact clinical issues including functional diagnosis and cell-specific drug delivery. Using a phage display approach, we have isolated 20-mer peptide ligands that bind to 3 different human lung tumor cell lines, NCI-H1299, NCI-H2009, and A549. The panning protocol is unbiased with no selection pressure towards binding a particular cellular receptor. The isolated phage bind to their target cells 24-300 times better than a control phage. Furthermore, the isolated peptides display remarkable cell-specificities and are able to discriminate between normal and cancerous cells as well as different lung tumor cells. The cell-specificities are not coincident with tumor classes indicating that the peptides are able to recognize cell-surface features that are not represented within the classification of tumor type. The isolated peptides are functional outside of the context of the phage and multimerization of the peptide increases its affinity for its given cell type, thus expanding their utility in clinical situations.
authors.affiliation: Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9185, USA.
authors.forename: Tsuksa
authors.initials: T
authors.lastname: Oyama
authors.forename: Kathryn F
authors.initials: KF
authors.lastname: Sykes
authors.forename: Kausar N
authors.initials: KN
authors.lastname: Samli
authors.forename: John D
authors.initials: JD
authors.lastname: Minna
authors.forename: Stephen Albert
authors.initials: SA
authors.lastname: Johnston
authors.forename: Kathlynn C
authors.initials: KC
authors.lastname: Brown
mesh_terms: D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification / Q000175:diagnosis*; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D019151:Peptide Library; D010455:Peptides