[Bone induction by human periodontal ligament fibroblasts transfected with bone morphogenetic protein 2 gene].
OBJECTIVE
To establish human periodontal ligament fibroblasts (HPDLFs) that express bone morphogenetic protein 2 and observe their osteogenesis in vivo, so as to provide fundamental data for future study on gene therapy of periodontal defect treatment.


METHODS
The phagemid expression vector for BMP-2 (pBK-B2) was transfected into HPDLFs by using Lipofect AMINE. The BMP-2 expression was determined by immunohistochemical ABC method. The BMP-2 gene transfected HPDLFs were injected into thigh muscle of nude mice under sterile condition. Specimens were collected in 21 days after injection and underwent normal procedures for fixation, decalcification, embedding, incising and staining with HE.


RESULTS
The results demonstrated that BMP-2 protein expressed in HPDLFs after BMP-2 gene transfection. The HPDLFs transfected with BMP-2 gene could induce new bone formation at the injection site.


CONCLUSIONS
The results indicated that BMP-2 gene was transfected successfully and expressed efficiently in HPDLFs. The HPDLFs transfected with BMP-2 gene are capable of inducing new bone formation in vivo.
Si
Xiaohui
X
Research Institute of Stomatology, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Liu
Zheng
Z
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000502:physiology; D002648:Child; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D014162:Transfection; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta