title: Identification of 6-aminochrysene photoproducts and study of the effect of a humic acid and riboflavin on its photolysis.
abstract: The effect of the photosensitizer riboflavin (0, 10, 25, 50, 100 microM) and a river humic acid (0, 20, 40, 80 ppm) on the photolysis of 6-aminochrysene (6AC) in 10 mM PBS solution (pH 7.0) was studied. The presence of riboflavin significantly enhanced photolysis rate of 6AC. The photo-transformation half-life of 6AC was 1 or 36 min, respectively, in the presence or absence of riboflavin. The humic acid inhibited the photo-transformation rate of 6AC. The photo-transformation half-lives of 6AC were 37, 56, 92 min at 20, 40, 80 ppm humic acid, respectively. By using LC-MS, the main 6AC photoproduct identified was 5,6-chrysene-quinone along with some minor products. Both 6AC and 5,6-chrysene-quinone exhibited photoinduced cytotoxicity. A photochemical transformation pathway for 6AC was derived.
authors.affiliation: Department of Biology, Jackson State University, Jackson, MS 39217, USA.
authors.forename: Kui
authors.initials: K
authors.lastname: Zeng
authors.forename: Huey-min
authors.initials: HM
authors.lastname: Hwang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Yu
mesh_terms: D002911:Chrysenes / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects*; D006812:Humic Substances; D010782:Photolysis / Q000187:drug effects* / Q000528:radiation effects*; D012256:Riboflavin / Q000494:pharmacology*
chemical_list: D002911:Chrysenes; D006812:Humic Substances; C100101:6-chrysenamine; D012256:Riboflavin