title: Differential alternative splicing expressions of telomerase reverse transcriptase in gastrointestinal cell lines.
abstract: Telomerase is a cellular RNA-dependent DNA polymerase that serves to maintain the tandem arrays of telomeric TTAGGG repeats at eukaryotic chromosome ends. One of the human telomerase components is hTERT, which has three alternative spliced sites that introduce eight isoforms of hTERT mRNA. The expression of these isoforms in gastrointestinal cell lines is unknown. We developed a PCR-based assay for detecting these splicing variants. In gastric and hepatocellular carcinoma cell lines, the gamma deletion variant and its combination variants, alpha- and gamma-, beta- and gamma-, and alpha-, beta- and gamma-deletion variants were frequently detected, while they were not detected in colorectal carcinoma cell lines. Our results provide important information of use for more detailed studies on the regulation of telomerase activity.
authors.affiliation: Center for Molecular Biology and Cytogenetics, SRL Inc., Tokyo 191-0002, Japan. knagao@srl.srl-inc.co.jp
authors.forename: Kumi
authors.initials: K
authors.lastname: Nagao
authors.forename: Kenji
authors.initials: K
authors.lastname: Katsumata
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Aizawa
authors.forename: Noboru
authors.initials: N
authors.lastname: Saito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hirata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Hikiji
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Koiwa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hisatomi
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D005770:Gastrointestinal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D019098:Telomerase