Effect of high-NaCl or high-KCl diet on hepatic Na+- and K+-receptor sensitivity and NKCC1 expression in rats.
We previously reported that the bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter (NKCC1) is involved in the hepatic Na+ and K+ sensor mechanism. In the present study, we examined the effects of a high-NaCl or high-KCl diet on hepatic Na+ and K+ receptor sensitivity and NKCC1 expression in the liver of Sprague-Dawley rats. RT-PCR and Western blots were used to measure NKCC1 mRNA and protein expression, respectively. Infusion of hypertonic NaCl or isotonic KCl + NaCl solutions into the portal vein increased hepatic afferent nerve activity (HANA) in a Na+ or K+ dose-dependent manner. After 4 wk on a high-NaCl or high-KCl diet, HANA responses were attenuated compared with animals fed a normal diet, and NKCC1 expression was reduced. These results show that a high-NaCl or high-KCl diet decreases NKCC1 expression in the liver, and it might cause a reduction in hepatic Na(+)- and K(+)-receptor sensitivity.
Tsuchiya
You
Y
Department of Physiology, Gifu University School of Medicine, Gifu 500-8705, Japan.
Nakashima
Shigeru
S
Banno
Yoshiko
Y
Suzuki
Yuichi
Y
Morita
Hironobu
H
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D006400:Hematocrit; D006439:Hemodynamics / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D008102:Liver Circulation / Q000187:drug effects; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D028021:Sodium-Potassium-Chloride Symporters / Q000096:biosynthesis*; D064506:Solute Carrier Family 12, Member 2; D014882:Water-Electrolyte Balance / Q000187:drug effects
D012333:RNA, Messenger; C578879:Slc12a2 protein, rat; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2; D012965:Sodium Chloride; D011189:Potassium Chloride; D012964:Sodium; D011188:Potassium