Induction of chromosome aberrations in cultured human lymphocytes treated with ethoxyquin.
The chromosomal aberration test was employed to investigate the effect in vitro of a known antioxidant and food preservative, ethoxyquin (EQ, 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline) on human chromosomes. The studies were undertaken because there are no published in vitro data on genotoxicity of EQ in mammalian cells and there are many reports pointing out that it may be harmful to animals and human beings. Lymphocytes obtained from three healthy donors were incubated with EQ (0.01-0.5mM) both with and without metabolic activation. Stability studies performed by HPLC analysis showed that EQ was stable under the conditions of the lymphocyte cultures. The results of the chromosome aberration assay showed that EQ induces chromosome aberrations: gaps and breaks as well as dicentrics and atypical translocation chromosomes.
Blaszczyk
A
A
Department of Cytogenetics and Plant Molecular Biology, University of Lódź, Banacha 12/16, 90-237, Lódź, Poland. ablasz@biol.uni.lodz.pl
Osiecka
R
R
Skolimowski
J
J
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002869:Chromosome Aberrations / Q000139:chemically induced*; D004355:Drug Stability; D005015:Ethoxyquin / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005520:Food Preservatives / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D009153:Mutagens / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D012044:Regression Analysis
D005520:Food Preservatives; D009153:Mutagens; D005015:Ethoxyquin