Correlation of oxidative stress with activity of matrix metalloproteinase in patients with coronary artery disease. Possible role for left ventricular remodelling.
OBJECTIVE
Oxidative stress is implicated in the progression of heart failure, and matrix metalloproteinase (MMP) activity is increased in patients with congestive heart failure. We examined the role of oxidative stress on MMP activity in humans.


RESULTS
We measured the MMP activity and the level of 8-iso-prostagandin F2alpha (8-iso-PGF2alpha), a specific and quantitative maker of oxidant stress, in the pericardial fluid (PF) in 47 consecutive patients with coronary artery disease who underwent coronary artery bypass surgery. Zymography of PF showed bands at 92-85kDa (MMP-9) and 72-65kDa (MMP-2). The MMP activity was expressed as the ratio to MMP-2 standard. MMP-2, MMP-9 and total gelatinolysis activities were positively correlated with left ventricular end-diastolic volume index (LVEDVI), and MMP-2 and total gelatinolysis activities were also positively correlated with LV end-systolic volume index. Moreover, MMP-2, MMP-9 and total gelatinolysis activities were all positively correlated with pericardial level of 8-iso-PGF2alpha. Also, LVDEVI was positively correlated with pericardial level of 8-iso-PGF2alpha.


CONCLUSIONS
Oxidative stress may play an important role in the regulation of MMP activity. Augmented MMP activity may be involved in the development of ventricular remodelling in patients with coronary artery disease.
Kameda
Kunihiko
K
The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan.
Matsunaga
Toshiro
T
Abe
Naoki
N
Hanada
Hiroyuki
H
Ishizaka
Hiroshi
H
Ono
Hirotsugu
H
Saitoh
Masayuki
M
Fukui
Kozo
K
Fukuda
Ikuo
I
Osanai
Tomohiro
T
Okumura
Ken
K
D000368:Aged; D003324:Coronary Artery Disease / Q000201:enzymology / Q000209:etiology*; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010496:Pericardium / Q000737:chemistry; D011454:Prostaglandins A / Q000032:analysis; D020257:Ventricular Remodeling / Q000502:physiology*
C117466:8-isoprostaglandin A2; D011454:Prostaglandins A; D020782:Matrix Metalloproteinases