title: A simple and sensitive chemiluminescence method for the determination of tiopronin for a pharmaceutical formulation.
abstract: Here we report a rather simple and convenient chemiluminescence (CL) method for the determination of tiopronin. It was based that tiopronin could greatly enhance CL between H2O2 and luminol in a basic alkaline solution. Light emission is intense, and even with a simple setup a high sensitivity could be achieved. The linear range was 3 mM-500 nM with a detection limit of 200 nM. Singlet oxygen and hydroxyl radical were suggested to be produced in this reaction and was responsible for the CL of tiopronin. As a preliminary application, this simple method has been successfully applied into the determination of tiopronin in a pharmaceutical formulation.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China. jzlu@shmu.edu.cn
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lu
authors.forename: Choiwan
authors.initials: C
authors.lastname: Lau
authors.forename: Shiroki
authors.initials: S
authors.lastname: Yagisawa
authors.forename: Kazuko
authors.initials: K
authors.lastname: Ohta
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kai
mesh_terms: D002626:Chemistry, Pharmaceutical; D008163:Luminescent Measurements; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D008625:Tiopronin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D004364:Pharmaceutical Preparations; D008625:Tiopronin