title: Contribution of mitochondria to cardiac muscle water/macromolecule proton magnetization transfer.
abstract: The contribution of mitochondria to water-macromolecule proton magnetization transfer (MT) was evaluated in porcine heart tissue. An examination of isolated mitochondria in suspension, at the same concentration as found in heart tissue, revealed MT effects very similar in magnitude and bandwidth to those in intact heart tissue. Disruption of the gross structure of the mitochondria by freeze-thawing or with detergent resulted in only approximately 25% decreases in MT, which suggests that the structure of the mitochondria is not critical for these effects. The current data indicate that mitochondria macromolecules contribute significantly to MT in the intact heart.
authors.affiliation: Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20817, USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Ward
authors.forename: Adam E
authors.initials: AE
authors.lastname: Schussheim
authors.forename: Robert S
authors.initials: RS
authors.lastname: Balaban
mesh_terms: D000818:Animals; D001692:Biological Transport; D001834:Body Water / Q000378:metabolism*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008279:Magnetic Resonance Imaging; D008929:Mitochondria, Heart / Q000502:physiology* / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D011522:Protons; D013552:Swine
chemical_list: D046911:Macromolecular Substances; D011522:Protons