2E3, a new marker that selectively identifies porcine CD4+ naive T cells.
We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are CD29low, CD45RAhigh, CD49dlow, CD11alow, CD18low, and SLA-II-. After mitogen activation CD4+2E3+ T cells express high levels of IL-2 mRNA, but only traces of IFN-gamma or IL-4 mRNA. Indeed a minor percentage of cells stained positive for IFN-gamma when assessed by flow cytometry. Moreover, CD4+2E3+ T cells did not proliferate in response to the recall antigen lysozyme, although they did efficiently to the mitogen ConA. By contrast, CD4+2E3- T cells show phenotypical and functional characteristics of primed cells. They express markers associated to a memory phenotype, respond to the recall antigen lysozyme, and produce high amounts of IFN-gamma and IL-4.
Revilla
C
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Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña, km 7, 28040 Madrid, Spain.
Rodríguez-Carreño
M P
MP
Alvarez
B
B
Chamorro
S
S
Alonso
L M
LM
Ezquerra
A
A
Alonso
F
F
Domínguez
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D007376:Interleukin-2; D003208:Concanavalin A; D015847:Interleukin-4; D015759:Ionomycin; D007371:Interferon-gamma; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate