title: Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells.
abstract: Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source of cardiomyocyte progenitors is of great interest to provide a usable model in vitro and new perspective in regenerative therapy. As adipose tissues were recently demonstrated to contain pluripotent stem cells, the emergence of cardiomyocyte phenotype from adipose-derived cells was investigated. We demonstrated that rare beating cells with cardiomyocyte features could be identified after culture of adipose stroma cells without addition of 5-azacytidine. The cardiomyocyte phenotype was first identified by morphological observation, confirmed with expression of specific cardiac markers, immunocytochemistry staining, and ultrastructural analysis, revealing the presence of ventricle- and atrial-like cells. Electrophysiological studies performed on early culture revealed a pacemaker activity of the cells. Finally, functional studies showed that adrenergic agonist stimulated the beating rate whereas cholinergic agonist decreased it. Taken together, this study demonstrated that functional cardiomyocyte-like cells could be directly obtained from adipose tissue. According to the large amount of this tissue in adult mammal, it could represent a useful source of cardiomyocyte progenitors.
authors.affiliation: UMR 5018 UPS CNRS, IFR31, Research Institute 31, France.
authors.forename: V
authors.initials: V
authors.lastname: Planat-Bénard
authors.forename: C
authors.initials: C
authors.lastname: Menard
authors.forename: M
authors.initials: M
authors.lastname: André
authors.forename: M
authors.initials: M
authors.lastname: Puceat
authors.forename: A
authors.initials: A
authors.lastname: Perez
authors.forename: J-M
authors.initials: JM
authors.lastname: Garcia-Verdugo
authors.forename: L
authors.initials: L
authors.lastname: Pénicaud
authors.forename: L
authors.initials: L
authors.lastname: Casteilla
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D018663:Adrenergic Agents / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D018678:Cholinergic Agents / Q000494:pharmacology; D002999:Clone Cells / Q000166:cytology; D006325:Heart Atria / Q000166:cytology; D006352:Heart Ventricles / Q000166:cytology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology*; D032386:Myoblasts, Cardiac / Q000166:cytology*; D009200:Myocardial Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype; D011433:Propranolol / Q000494:pharmacology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D018663:Adrenergic Agents; D018678:Cholinergic Agents; D012313:RNA; D001285:Atropine; D002217:Carbachol; D011433:Propranolol; D007545:Isoproterenol