title: Ameliorating effect of beta-carotene on ethylmethane sulphonate-induced genotoxicity in the fish Oreochromis mossambicus.
abstract: Genotoxic effects have been assessed in the fish Oreochromis mossambicus treated separately and conjointly with 0.2% ethylmethane sulphonate (EMS) and 0.05% beta-carotene (BC) during five different time periods (6, 24, 48, 72 and 96 h) by analysis of endpoints such as chromosome aberrations, abnormal red blood cell nuclei, abnormal sperm morphology and protein contents (both qualitative and quantitative) of selected tissues, viz. muscle, heart, eye, brain, gill, liver, spleen and kidney. In addition, the relative efficacy of three doses of BC 0.02, 0.05 and 0.1%, in ameliorating genotoxic effects of 0.2% EMS was also tested after a treatment period of 48 h. EMS caused chromosomal aberrations, nuclear anomalies in red blood cells, abnormal sperm morphology and an apparent alteration of protein synthesis in various tissues. Some of these genotoxic effects of EMS appeared to be ameliorated by all three doses of BC, of which the 0.02% dose showed a marginally better efficacy.
authors.affiliation: Department of Zoology, University of Kalyani, Kalyani, 741235, West Bengal, India.
authors.forename: Bibhas
authors.initials: B
authors.lastname: Guha
authors.forename: Anisur Rahman
authors.initials: AR
authors.lastname: Khuda-Bukhsh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000139:chemically induced* / Q000187:drug effects; D004347:Drug Interactions; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009928:Organ Specificity; D011506:Proteins / Q000378:metabolism; D013077:Sperm Head / Q000187:drug effects; D017210:Tilapia / Q000235:genetics*; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D009153:Mutagens; D011506:Proteins; D019207:beta Carotene; D005020:Ethyl Methanesulfonate