Microvascular basal lamina damage in thromboembolic stroke in a rat model.
We investigated microvascular damage in areas with magnetic resonance imaging (MRI)-defined apparent diffusion coefficient reduction (ADC-R) in a rat model of thromboembolic occlusion of the middle cerebral artery. Rats received either intracarotid recombinant tissue plasminogen activator (rt-PA) or saline. Microvascular basal lamina damage was quantified by immunohistochemical staining of collagen type IV and by videoimaging analysis. ADC-R positive basal ganglia (cortical) areas showed a significant reduction of stained microvascular area by 15+/-6% (8+/-7%) and the microvascular density by 13+/-5% (8+/-6%) of that on the non-ischemic control side (P<0.001). There were no significant microvascular differences between rats given rt-PA or saline, or between those with or without angiographically proven recanalization. This study reports for the first time that microvascular basal lamina damage in experimental thromboembolic stroke is confined to regions with ADC-R in MRI.
Vosko
Milan R
MR
Department of Neurology, Ludwig-Maximilians University, Klinikum Grosshadern, Marchioninistrasse 15, D-81377 Munich, Germany.
Busch
Elmar
E
Burggraf
Dorothe
D
Bültemeier
Gundula
G
Hamann
Gerhard F
GF
D000792:Angiography / Q000379:methods; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D001485:Basement Membrane / Q000473:pathology*; D002525:Cerebellar Cortex / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D008279:Magnetic Resonance Imaging / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000209:etiology / Q000473:pathology*; D013923:Thromboembolism / Q000209:etiology / Q000473:pathology*; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use
D024043:Collagen Type II; D010959:Tissue Plasminogen Activator