title: Developmentally regulated expression of the mouse homologues of the potassium channel encoding genes m-erg1, m-erg2 and m-erg3.
abstract: Deciphering the expression pattern of K+ channel encoding genes during development can help in the understanding of the establishment of cellular excitability and unravel the molecular mechanisms of neuromuscular diseases. We focused our attention on genes belonging to the erg family, which is deeply involved in the control of neuromuscular excitability in Drosophila flies and possibly other organisms. Both in situ hybridisation and RNase Protection Assay experiments were used to study the expression pattern of mouse (m)erg1, m-erg2 and m-erg3 genes during mouse embryo development, to allow the pattern to be compared with their expression in the adult. M-erg1 is first expressed in the heart and in the central nervous system (CNS) of embryonic day 9.5 (E9.5) embryos; the gene appears in ganglia of the peripheral nervous system (PNS) (dorsal root (DRG) and sympathetic (SCG) ganglia, mioenteric plexus), in the neural layer of retina, skeletal muscles, gonads and gut at E13.5. In the adult m-erg1 is expressed in the heart, various structures of the CNS, DRG and retina. M-erg2 is first expressed at E9.5 in the CNS, thereafter (E13.5) in the neural layer of retina, DRG, SCG, and in the atrium. In the adult the gene is present in some restricted areas of the CNS, retina and DRG. M-erg3 displayed an expression pattern partially overlapping that of m-erg1, with a transitory expression in the developing heart as well. A detailed study of the mouse adult brain showed a peculiar expression pattern of the three genes, sometimes overlapping in different encephalic areas.
authors.affiliation: Department of Experimental Pathology and Oncology, University of Firenze, Viale G.B.Morgagni 50, 50134, Florence, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Polvani
authors.forename: Alessio
authors.initials: A
authors.lastname: Masi
authors.forename: Serena
authors.initials: S
authors.lastname: Pillozzi
authors.forename: Laura
authors.initials: L
authors.lastname: Gragnani
authors.forename: Olivia
authors.initials: O
authors.lastname: Crociani
authors.forename: Massimo
authors.initials: M
authors.lastname: Olivotto
authors.forename: Andrea
authors.initials: A
authors.lastname: Becchetti
authors.forename: Enzo
authors.initials: E
authors.lastname: Wanke
authors.forename: Annarosa
authors.initials: A
authors.lastname: Arcangeli
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
chemical_list: D000072237:ERG1 Potassium Channel; C488358:Erg3 protein, mouse; D051638:Ether-A-Go-Go Potassium Channels; C000606915:Kcnh2 protein, mouse; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger