title: Effects of chronic hypogonadism on corticosterone secretion and cyclic AMP production in male rat adrenocortical cells.
abstract: OBJECTIVE
  To determine the secretion of corticosterone (CCS) both in vivo and in vitro during different intervals after orchidectomy in male rats.
  
  
  METHODS
  Three- and 12-month-old rats had been orchidectomized 0, 3, 6, or 9 months before decapitation.
  
  
  RESULTS
  Orchidectomy increased the concentrations of plasma CCS, the basal release of CCS, and the adenosine 3', 5'-cyclic monophosphate (cAMP) production in rat zona fasciculata reticularis (ZFR) cells. The forskolin/3-isobutyl-l-methylxanthine-stimulated releases of CCS and cAMP production by ZFR cells were higher in rats with chronic hypogonadism. The CCS release from ZFR cells of orchidectomized rat was not altered by 8-bromo-cAMP treatment. Orchidectomy enhanced the stimulatory effect of deoxycorticosterone on CCS release in ZFR cells.
  
  
  CONCLUSIONS
  These results suggest that orchidectomy-related increases of CCS secretion in rats are associated with an increase of adenylate cyclase activity, cAMP generation, and 11-beta-hydroxylase activity in ZFR cells.
authors.affiliation: Department of Physical Therapy, Hungkuang University, Taichung, Taiwan, ROC. lo@sunrise.hk.edu.tw
authors.forename: Ming-Jae
authors.initials: MJ
authors.lastname: Lo
authors.forename: Mei-Mei
authors.initials: MM
authors.lastname: Kau
authors.forename: Paulus S
authors.initials: PS
authors.lastname: Wang
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000302:Adrenal Cortex / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000097:blood / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D007006:Hypogonadism / Q000097:blood / Q000503:physiopathology*; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood; D015383:Zona Fasciculata / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology
chemical_list: D015124:8-Bromo Cyclic Adenosine Monophosphate; D013739:Testosterone; D000242:Cyclic AMP; D003345:Corticosterone