[Expression of matrix metalloproteinase-3 and tissue inhibitor metalloproteinases-1 in regenerated rabbit bone after mandibular osteodistraction].
OBJECTIVE
To observe the expression patterns of matrix metalloproteinase-3 and its inhibitor, tissue inhibitor metalloproteinases (TIMP)-1, in remodeling phase of mandibular distraction osteogenesis.


METHODS
Bilateral mandibular osteotomies were performed in 8 mature rabbits. The mandibles were lengthened 6 mm using a custom-made distractor with a rate of 1 mm/d. All animals were killed in 4 weeks after completion of distraction. The distracted calluses were harvested and processed for histology and immunohistochemistry of MMP-3 and TIMP-1.


RESULTS
Elevated expression of both MMP-3 and TIMP-1 was found in the distracted calluses resulting from mandibular lengthening. Positive staining for MMP-3 was seen in the osteoblasts and osteocytes, and TIMP-1 was mainly localized in osteoblasts.


CONCLUSIONS
MMP-3 and TIMP-1 appear to play important roles in the remodeling of new bone created by mandibular distraction osteogenesis.
Zou
Shujuan
S
Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Wang
Zhiguo
Z
Hu
Jing
J
D000818:Animals; D001861:Bone Regeneration; D016723:Bone Remodeling; D005260:Female; D008297:Male; D008334:Mandible / Q000166:cytology / Q000378:metabolism / Q000601:surgery*; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism; D019857:Osteogenesis, Distraction; D011817:Rabbits; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis*
D019715:Tissue Inhibitor of Metalloproteinase-1; D019278:Matrix Metalloproteinase 3