title: [Study on Calpain10 gene polymorphism in Chinese type 2 diabetes families].
abstract: OBJECTIVE
  To detect the Calpain10 gene polymorphisms in North China families with type 2 diabetes and to investigate their association with type 2 diabetes.
  
  
  METHODS
  PCR-RFLP method was used to test the polymorphisms of Calpain10 SNP43 (G/A) and SNP19 (1/2) in 801 individuals from 218 type 2 diabetes mellitus (DM) families, 211 type 2 diabetes patients without family history, and 127 normal control subjects in northern China.
  
  
  RESULTS
  (1) The Calpain 10 SNP43 "G" allele frequency was 91.9% in the type 2 diabetic patients without family history, 92.7% in the probands from the type 2 diabetes families without linkage between the onset of DM and SNP43 site, and 95.3% in the probands from type 2 diabetes families with linkage evidence at SNP43, all significantly higher than that in the controls (85.8%, chi(2) = 6.39, df = 1,P = 0.011; chi(2) = 8.437,df = 1, P < 0.01); and chi(2) = 16.49, df = 1, P < 0.01). The distribution of polymorphism of the SNP19 site was not significantly different between the patients and control subjects. (2) Logistic regression analysis adjusted by BMI, sex and age showed that SNP43 G/G genotype was associated with type 2 diabetes. The odds ratios of the three group were OR = 1.78, P = 0.045; OR = 2.53, P = 0.008; OR = 4.32, P = 0.000 respectively.
  
  
  CONCLUSIONS
  SNP43 site of Calpain10 gene is related to type 2 diabetes. Calpain 10 gene may be a related gene of type 2 diabetes in Chinese.
authors.affiliation: Department of Gerontology, People's Hospital of Peking University, Beijing 100044, China.
authors.forename: Ling-xia
authors.initials: LX
authors.lastname: Chen
authors.forename: Li-nong
authors.initials: LN
authors.lastname: Ji
authors.forename: Xue-yao
authors.initials: XY
authors.lastname: Han
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D002154:Calpain / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
chemical_list: D002154:Calpain; C415197:calpain 10