Identification of proteins associated with murine gammaherpesvirus 68 virions.
Murine gammaherpesvirus 68 (MHV68 [also known as gammaHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identified by mass spectrometry. Five nucleocapsid protein homologues, the tegument protein homologue encoded by open reading frame (ORF) 75c, and envelope glycoproteins B and H were detected. In addition, gene products from MHV68 ORF20, ORF24, ORF28, ORF45, ORF48, and ORF52 were identified in association with virions, suggesting that these gammaherpesvirus genes are involved in the early phase of infection or virion assembly and egress.
Bortz
Eric
E
Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.
Whitelegge
Julian P
JP
Jia
Qingmei
Q
Zhou
Z Hong
ZH
Stewart
James P
JP
Wu
Ting-Ting
TT
Sun
Ren
R
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D020285:Cryoelectron Microscopy; D018142:Gammaherpesvirinae / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure*; D019065:Virus Assembly
D010455:Peptides; D014764:Viral Proteins