Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends.
Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajstić, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA3 (chromomycin A3) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.
Puizina
Jasna
J
Gregor Mendel Institute ofMolecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria. jasna.puizina@gmi.oeaw.ac.at
Weiss-Schneeweiss
Hanna
H
Pedrosa-Harand
Andrea
A
Kamenjarin
Juraj
J
Trinajstic
Ivo
I
Riha
Karel
K
Schweizer
Dieter
D
D000818:Animals; D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D019668:Liliaceae / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D014714:Vertebrates / Q000235:genetics