Molecular and antigenic characterization of Bordetella bronchiseptica isolated from a wild southern sea otter (Enhydra lutris nereis) with severe suppurative bronchopneumonia.
Bordetella bronchiseptica was isolated in pure culture from the lung, abdomen, and intestine of a wild free-ranging southern sea otter (Enhydra lutris nereis) with severe, suppurative bronchopneumonia. Immunohistochemistry, using antiserum raised to B. bronchiseptica, revealed strong positive staining of bacteria attached to bronchial ciliated epithelia as well as scattered positive staining in affected alveoli. Western blot analysis demonstrated that virulence factors, filamentous hemagglutinin, pertactin, and adenylate cyclase toxin are produced by the sea otter B. bronchiseptica isolate. Ribotype analysis using Pvu II restriction digests indicated that this isolate is most similar to strains commonly obtained in domestic dogs and cats.
Staveley
Clare M
CM
California Department of Fish and Game, Marine Wildlife Veterinary Care and Research Center, Santa Cruz, CA 95060, USA.
Register
Karen B
KB
Miller
Melissa A
MA
Brockmeier
Susan L
SL
Jessup
David A
DA
Jang
Spencer
S
D000818:Animals; D000835:Animals, Wild; D015153:Blotting, Western; D001885:Bordetella Infections / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D016950:Bordetella bronchiseptica / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001996:Bronchopneumonia / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D008168:Lung / Q000382:microbiology; D010041:Otters / Q000382:microbiology*; D021521:Ribotyping