title: The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization.
abstract: The nature of genetic alterations in bilateral breast cancer (BBC) associated with the distinctive development of a second primary tumor or a metastatic lesion is not clearly established. In this study, patterns of promoter methylation and gene copy number changes were assessed for their utility in the distinction of two types of BBC (synchronous and metachronous). Seven cases of synchronous and five cases of metachronous breast cancer tissues were used in X chromosome inactivation assay to assess the methylation pattern of human androgen receptor gene. X chromosome inactivation assay alone did not provide enough information to distinguish the genetic origins of synchronous and metachronous BBC. When four pairs of paraffin-embedded BBC tissues were used in cDNA array-based CGH with placenta DNA as a reference, higher DNA copy number changes were observed from metachronous pairs (9.0%) than from synchronous pairs (3.1%). From the two cases of metachronous pairs tested, 44 genes were found to be commonly modulated in gene copy numbers in a cancer detected later.
authors.affiliation: Cancer Metastasis Research Center, Yonsei University, College of Medicine, Seoul 120-752, Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Seo
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Rha
authors.forename: Sang Hwa
authors.initials: SH
authors.lastname: Yang
authors.forename: Sang Cheol
authors.initials: SC
authors.lastname: Kim
authors.forename: Gui Youn
authors.initials: GY
authors.lastname: Lee
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Park
authors.forename: Woo Ick
authors.initials: WI
authors.lastname: Yang
authors.forename: Joong Bae
authors.initials: JB
authors.lastname: Ahn
authors.forename: Byung Woo
authors.initials: BW
authors.lastname: Park
authors.forename: Hyun Cheol
authors.initials: HC
authors.lastname: Chung
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D041321:Chromosomes, Human, X; D018076:DNA, Complementary; D004303:Dosage Compensation, Genetic; D005260:Female; D018628:Gene Dosage; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D018076:DNA, Complementary