Improvement of reverse transcription PCR by RNase H.
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5' terminal. Furthermore, the RNase H treatment improved the sensitivity of RT-PCR amplification against a general target.
Kitabayashi
Masao
M
Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
Esaka
Muneharu
M
D001483:Base Sequence; D017931:DNA Primers; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016914:Ribonuclease H / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
D017931:DNA Primers; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H