Construction of vectors for inducible gene expression in Lactobacillus sakei and L plantarum.
We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum.
Sørvig
Elisabeth
E
Matforsk, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. elisabeth.sorvig@matforsk.no
Grönqvist
Sonja
S
Naterstad
Kristine
K
Mathiesen
Geir
G
Eijsink
Vincent G H
VG
Axelsson
Lars
L
D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D010957:Plasmids
D005966:Glucuronidase