Determination of DNA guanine sites forming photo-adducts with Ru(II)-labeled oligonucleotides; DNA polymerase inhibition by the resulting photo-crosslinking.
The influence of the distance between the anchoring site of the tethered [Ru(TAP)(2)dip](2+) complex (TAP=1,4,5,8-tetraazaphenanthrene; dip=4,7-diphenyl-1,10-phenanthroline) on a probe sequence and the guanines of the complementary target strand was studied by (1) the luminescence quenching of the complex (by electron transfer) and (2) the oligodeoxyribonucleotide adduct (ODN adduct) formation which results in photo-crosslinking of the two strands. Moving the guanine moieties away from the complex induces an important decrease of the efficiency of both processes, but clearly affects the ODN adduct formation more specifically than the quenching process. From these results, we determined the positions of the guanine bases in the duplex ODN that are able to form a photo-adduct with the tethered complex. We also examined the possible competition between a long-range hole migration in the duplex ODN and the formation of a photo-adduct by using a sequence labeled with the complex at the 5'-phosphate end. Such a hole migration appears to be inefficient as compared to the ODN adduct formation. Finally, we studied the influence of the photo-crosslinking on the function of two different DNA polymerases. A 17-mer Ru(II)-labeled ODN was hybridized to its complementary sequence located on the 5'-side of a 40-mer matrix. After illumination, the elongation of a 13-mer DNA primer hybridized to the 3'-extremity of the same matrix was stopped at a position corresponding to the formation of the ODN adduct.
Lentzen
Olivier
O
Organic Chemistry and Photochemistry, Laboratoire Européen Associé Ingénierie Biomoléculaire, Université Libre de Bruxelles, 50 Avenue F.D. Roosevelt, CP 160/08, 1050, Brussels, Belgium.
Defrancq
Eric
E
Constant
Jean-François
JF
Schumm
Stephan
S
García-Fresnadillo
David
D
Moucheron
Cécile
C
Dumy
Pascal
P
Kirsch-De Mesmaeker
Andrée
A
D000970:Antineoplastic Agents / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry* / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D019951:DNA Polymerase beta / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D019791:Guanidine / Q000032:analysis* / Q000737:chemistry; D008027:Light; D015335:Molecular Probes / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010777:Photochemistry; D012428:Ruthenium / Q000737:chemistry*
D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D018736:DNA Adducts; D015335:Molecular Probes; D009838:Oligodeoxyribonucleotides; D012428:Ruthenium; D004256:DNA Polymerase I; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase; D019791:Guanidine