A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603.
A new Bacillus licheniformis strain, 603, isolated from a mixture of drilling fluid and subsurface thermal water, has been found to produce a cyclic lipopeptide which is released into cultural medium as well as present in cells as the major lipid constituent (57% of the total cell lipids extractable with 2:1 chloroform-methanol). The quantitative ratio of the extracellular and intracellular lipopeptide has been estimated as 23:10. The metabolite represents a heptapeptide, L-Asp-->L-Leu-->L-Leu-->L-Val-->L-Val-->L-Glu-->L-Leu, N-acylated to the N-terminal amino acid, L-Asp, by a 3-hydroxy fatty acid (from 13:0 to 17:0 with n-, iso-, and anteiso-chains), the 3-OH group of which is esterified by the C-terminal amino acid, L-Leu. The chemical structure of the lipopeptide has been established by means of infrared (IR), 1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionisation (ESI) mass spectrometry (MS), including secondary ion mass spectrometry, along with chemical and enzymatic degradation. Although a diversity of similar metabolites synthesised by various B. licheniformis strains are presently known, such a structure has not been reported thus far. Added to the growth medium of strain 603 at the concentration of 1.6 microg/ml, the lipopeptide prevents adhesion of cells to a glass surface. Also, it exhibits a considerable growth-inhibiting activity against Corynebacterium variabilis and a much lower activity against Acinetobacter sp.
Batrakov
Stanislav G
SG
Russian Research Centre Hydrobios of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russia.
Rodionova
Tatiana A
TA
Esipov
Stanislav E
SE
Polyakov
Nikita B
NB
Sheichenko
Vladimir I
VI
Shekhovtsova
Nina V
NV
Lukin
Sergey M
SM
Panikov
Nikolai S
NS
Nikolaev
Yuri A
YA
D000150:Acinetobacter / Q000187:drug effects / Q000254:growth & development; D000595:Amino Acid Sequence; D001407:Bacillus / Q000737:chemistry* / Q000302:isolation & purification; D001422:Bacterial Adhesion / Q000187:drug effects*; D003352:Corynebacterium / Q000187:drug effects / Q000254:growth & development; D008074:Lipoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012988:Soil Microbiology; D013057:Spectrum Analysis
D008074:Lipoproteins; D010456:Peptides, Cyclic