title: Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro.
abstract: Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. In addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC.
authors.affiliation: First Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokushima University, 3-18-15 Kuramoto, Tokushima 770-8504, Japan. yuasa@dent.tokushima-u.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yuasa
authors.forename: Youji
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Kunio
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takechi
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Momota
authors.forename: Seiko
authors.initials: S
authors.lastname: Tatehara
authors.forename: Masaru
authors.initials: M
authors.lastname: Nagayama
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001843:Bone Cements / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D008968:Molecular Conformation; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D006882:Hydroxyapatites