title: Yeast genes that enhance the toxicity of a mutant huntingtin fragment or alpha-synuclein.
abstract: Genome-wide screens were performed in yeast to identify genes that enhance the toxicity of a mutant huntingtin fragment or of alpha-synuclein. Of 4850 haploid mutants containing deletions of nonessential genes, 52 were identified that were sensitive to a mutant huntingtin fragment, 86 that were sensitive to alpha-synuclein, and only one mutant that was sensitive to both. Genes that enhanced toxicity of the mutant huntingtin fragment clustered in the functionally related cellular processes of response to stress, protein folding, and ubiquitin-dependent protein catabolism, whereas genes that modified alpha-synuclein toxicity clustered in the processes of lipid metabolism and vesicle-mediated transport. Genes with human orthologs were overrepresented in our screens, suggesting that we may have discovered conserved and nonoverlapping sets of cell-autonomous genes and pathways that are relevant to Huntington's disease and Parkinson's disease.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle, WA 98195-7280, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Willingham
authors.forename: Tiago Fleming
authors.initials: TF
authors.lastname: Outeiro
authors.forename: Michael J
authors.initials: MJ
authors.lastname: DeVit
authors.forename: Susan L
authors.initials: SL
authors.lastname: Lindquist
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Muchowski
mesh_terms: D001692:Biological Transport; D017353:Gene Deletion; D005800:Genes, Fungal; D006801:Humans; D000071058:Huntingtin Protein; D050356:Lipid Metabolism; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology*; D015538:Nitrosation; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D009997:Osmotic Pressure; D018384:Oxidative Stress; D017510:Protein Folding; D011506:Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D051843:Synucleins; D014170:Transformation, Genetic; D025801:Ubiquitin / Q000378:metabolism; D051844:alpha-Synuclein
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; C497604:SNCA protein, human; D051843:Synucleins; D025801:Ubiquitin; D051844:alpha-Synuclein