Bone marrow transplant completely rescues hematolymphoid defects in STAT5A/5B-deficient mice.
OBJECTIVE
STAT5A/5B-deficient mice are recognized to manifest defects in multiple cell types and tissues. In particular, the hematopoietic defects in these mice are widespread, affecting multiple lineages and multiple stages of development. Previous studies indicate that deficiencies intrinsic to hematopoietic cells contribute substantially to the observed defects. However, in light of the broad physiologic effects of STAT5 in the context of the organism outside the blood system, we wished to investigate the possibility of STAT5-dependent environmental influence of nonhematopoietic origin on hematopoietic development in these mice.


METHODS
We transplanted wild-type bone marrow into STAT5A/5B-deficient mice to determine the effects of loss of STAT5 in nonhematopoietic tissue on hematopoietic development.


RESULTS
We observed that transplantation of wild-type marrow completely corrects hematopoietic defects in STAT5A/5B-deficient recipient mice, including peripheral blood counts, bone marrow cellularity, and reductions in specific progenitor subsets.


CONCLUSIONS
These results indicate that the important role of STAT5 in hematolymphoid development are mediated directly through effects on hematopoietic cells and not indirectly.
Snow
Jonathan W
JW
Gladstone Institute of Virology and Immunology, Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94141-9100, USA.
Abraham
Ninan
N
Ma
Melissa C
MC
Goldsmith
Mark A
MA
D000818:Animals; D001773:Blood Cells / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006413:Hematopoietic System / Q000254:growth & development; D008208:Lymphatic System / Q000254:growth & development; D051379:Mice; D018345:Mice, Knockout; D008894:Milk Proteins; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics
D004268:DNA-Binding Proteins; D008894:Milk Proteins; D050799:STAT5 Transcription Factor; C494093:Stat5a protein, mouse; D015534:Trans-Activators