Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an alpha-L-rhamnosidase of enological interest.
The Aspergillus aculeatus rhaA gene encoding an alpha-L-rhamnosidase has been expressed in both laboratory and industrial wine yeast strains. Wines produced in microvinifications, conducted using a combination of the genetically modified industrial strain expressing rhaA and another strain expressing a beta-glucosidase, show increased content mainly of the aromatic compound linalool.
Manzanares
Paloma
P
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot, 46100 Valencia, Spain.
Orejas
Margarita
M
Gil
José Vicente
JV
De Graaff
Leo H
LH
Visser
Jaap
J
Ramón
Daniel
D
D000080462:Acyclic Monoterpenes; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001709:Biotechnology / Q000379:methods; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D027843:Vitis / Q000378:metabolism / Q000382:microbiology; D014920:Wine / Q000382:microbiology*; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; C018584:linalool; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C022525:alpha-L-rhamnosidase