title: The simultaneous in vivo perilymphatic perfusion of avian auditory and vestibular end organs.
abstract: Perilymphatic perfusion is a method that allows the control of fluid parameters throughout the perilymphatic space of the inner ear. We have evaluated a new method for continuous perilymphatic perfusion of the auditory and vestibular end organs with artificial perilymph (APL) in chickens. Perfusate temperature (39.0 degrees C), pH (7.4), osmolarity (328 +/- 2 mosm), and flow rate (2 microl/min) were carefully controlled. Independent functional tests of vestibular and auditory sensory systems were made throughout perfusion periods by recording peripheral compound action potentials (CAPs). The recordings provided a means of monitoring the status of hair cell transduction, synaptic transmission and collective primary afferent activation in response to auditory or vestibular gravity receptor stimuli. Auditory and vestibular responses were stable during perfusion. No significant changes occurred in vestibular or auditory CAP amplitudes during long-term perfusion (50-80 min, n=7) and responses remained stable in one animal perfused for over 3 h. To our knowledge, there have been no reports evaluating vestibular function under these conditions. This technique enables us to systematically study receptor pharmacology in the peripheral vestibular and auditory systems virtually simultaneously in vivo. The model is well suited for use in the study of the pharmacology and toxicology of inner ear sensory systems.
authors.affiliation: Department of Physiology, University of Missouri-Columbia, Columbia, MO 65203, USA.
authors.forename: Shunda R
authors.initials: SR
authors.lastname: Irons-Brown
authors.forename: Sherri M
authors.initials: SM
authors.lastname: Jones
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Jones
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002645:Chickens / Q000502:physiology*; D003051:Cochlea / Q000187:drug effects / Q000502:physiology*; D005072:Evoked Potentials, Auditory / Q000187:drug effects / Q000502:physiology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018069:Hair Cells, Vestibular / Q000502:physiology; D010477:Perfusion / Q000379:methods*; D010498:Perilymph / Q000502:physiology*; D011930:Reaction Time / Q000187:drug effects; D013995:Time; D014722:Vestibule, Labyrinth / Q000187:drug effects / Q000502:physiology*
chemical_list: D018691:Excitatory Amino Acid Antagonists; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione