Immunohistolocalization of carbonic anhydrase isozyme (CA-VI) in bovine mammary glands.
The localization of bovine carbonic anhydrase isozyme VI (CA-VI) was examined immunohistochemically in bovine mammary glands during early lactation period (after 2-3 days of postpartum) and dry period (at about 2 months preparturition in adults), and young calves (at 30 and 150 days after birth) using specific CA-VI antiserum. The immunoreaction for anti-CA-VI antiserum was very weak in the mammary glands in young (prepubescent) calves. In dry period, CA-VI was also weakly expressed in secretory epithelial (acinar) and ductal cells. In contrast, the reaction was intense in mammary gland cells in early lactation period. Dot blotting analysis indicated that anti-CA-VI reacted positively to beastings and mature saliva, but weakly or not at all to milk during the dry period or calf saliva, respectively. The intense expression of CA-VI in the mammary glands in early lactation period might compensate for low levels of secretion from functionally and structurally immature salivary glands in young calves.
Ichihara
Nobutsune
N
Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara-shi, Kanagawa, Japan.
Asari
Masao
M
Kasuya
Tamae
T
Susaki
Etsuko
E
Matsui
Kumi
K
Nishita
Toshiho
T
Amasaki
Hajime
H
D000818:Animals; D030741:Carbonic Anhydrase IV / Q000302:isolation & purification*; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000302:isolation & purification; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D012469:Salivary Glands / Q000378:metabolism
D007527:Isoenzymes; D030741:Carbonic Anhydrase IV