title: Incorporation of lycopene into chylomicron remnant-like particles enhances their induction of lipid accumulation in macrophages.
abstract: Lipid accumulation in macrophages exposed to chylomicron remnant-like particles containing the dietary antioxidant lycopene was investigated. After incubation with THP-1 macrophages (48h), chylomicron remnant-like particles containing lycopene (lycCRLPs) as compared to those without (CRLPs) caused significantly more lipid accumulation in the cells, and this was due to increases in both the triacylglycerol (+100%) and cholesterol (+62%) content. In addition, expression of mRNA for diacylglycerol acyltransferase (DGAT), a key enzyme in triacylglycerol synthesis, was significantly decreased by lycCRLPs, but not CRLPs. These findings suggest that lycopene from the diet may promote, rather than retard, lipid accumulation in macrophages during its transport in the blood in chylomicron remnants.
authors.affiliation: Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St., NW1 0TU, London, UK.
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Moore
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Napolitano
authors.forename: Antonella
authors.initials: A
authors.lastname: Prosperi
authors.forename: Michael
authors.initials: M
authors.lastname: Avella
authors.forename: Keith E
authors.initials: KE
authors.lastname: Suckling
authors.forename: Elena
authors.initials: E
authors.lastname: Bravo
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Botham
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001057:Apolipoproteins E / Q000737:chemistry / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D053298:Chylomicron Remnants; D002914:Chylomicrons / Q000737:chemistry* / Q000378:metabolism*; D051048:Diacylglycerol O-Acyltransferase; D006801:Humans; D050356:Lipid Metabolism; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism; D000077276:Lycopene; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism; D002785:Sterol O-Acyltransferase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001057:Apolipoproteins E; D053298:Chylomicron Remnants; D002914:Chylomicrons; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011973:Receptors, LDL; D002338:Carotenoids; D000217:Acyltransferases; C494881:DGAT1 protein, human; D051048:Diacylglycerol O-Acyltransferase; D002785:Sterol O-Acyltransferase; D000077276:Lycopene