title: Correlation of oxidative stress with activity of matrix metalloproteinase in patients with coronary artery disease. Possible role for left ventricular remodelling.
abstract: OBJECTIVE
  Oxidative stress is implicated in the progression of heart failure, and matrix metalloproteinase (MMP) activity is increased in patients with congestive heart failure. We examined the role of oxidative stress on MMP activity in humans.
  
  
  RESULTS
  We measured the MMP activity and the level of 8-iso-prostagandin F2alpha (8-iso-PGF2alpha), a specific and quantitative maker of oxidant stress, in the pericardial fluid (PF) in 47 consecutive patients with coronary artery disease who underwent coronary artery bypass surgery. Zymography of PF showed bands at 92-85kDa (MMP-9) and 72-65kDa (MMP-2). The MMP activity was expressed as the ratio to MMP-2 standard. MMP-2, MMP-9 and total gelatinolysis activities were positively correlated with left ventricular end-diastolic volume index (LVEDVI), and MMP-2 and total gelatinolysis activities were also positively correlated with LV end-systolic volume index. Moreover, MMP-2, MMP-9 and total gelatinolysis activities were all positively correlated with pericardial level of 8-iso-PGF2alpha. Also, LVDEVI was positively correlated with pericardial level of 8-iso-PGF2alpha.
  
  
  CONCLUSIONS
  Oxidative stress may play an important role in the regulation of MMP activity. Augmented MMP activity may be involved in the development of ventricular remodelling in patients with coronary artery disease.
authors.affiliation: The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Kameda
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Naoki
authors.initials: N
authors.lastname: Abe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hanada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishizaka
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ono
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saitoh
authors.forename: Kozo
authors.initials: K
authors.lastname: Fukui
authors.forename: Ikuo
authors.initials: I
authors.lastname: Fukuda
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osanai
authors.forename: Ken
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000368:Aged; D003324:Coronary Artery Disease / Q000201:enzymology / Q000209:etiology*; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010496:Pericardium / Q000737:chemistry; D011454:Prostaglandins A / Q000032:analysis; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: C117466:8-isoprostaglandin A2; D011454:Prostaglandins A; D020782:Matrix Metalloproteinases