Analysis of self-assembled monolayer interfaces by electrospray mass spectrometry: a gentle approach.
A general mass spectrometry technique for the characterization of alkanethiol-modified surfaces is presented. Alkanethiol self-assembled onto a gold surface (in this case, peptides were attached to the gold surface via a thiolate bond) was reductively desorbed in 0.05 M KOH in the presence of octadecyl-derivatized silica gel. The peptide adsorbed onto the silica gel, whereupon it could be filtered, washed to remove any salts, and then eluted using a mixture of 4:1 v/v methanol/water. The eluant containing the peptide was injected into a Fourier transform ion-cyclotron resonance mass spectrometer (FTICR/MS) via electrospray ionization. The spectrum showed no fragmentation of the peptide, demonstrating the gentleness of the technique. This simple procedure is not limited to FTICR/MS and could be adapted to other mass spectrometers.
Yang
Wenrong
W
School of Chemical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
Zhang
Rui
R
Willett
Gary D
GD
Hibbert
D Brynn
DB
Gooding
J Justin
JJ
D006046:Gold / Q000032:analysis* / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D010455:Peptides; D058428:Silica Gel; D006046:Gold; D012822:Silicon Dioxide