Structure of viscotoxin A3: disulfide location from weak SAD data.
The crystal structure of viscotoxin A3 (VT A3) extracted from European mistletoe (Viscum album L.) has been solved using the anomalous diffraction of the native S atoms measured in-house with Cu Kalpha radiation to a resolution of 2.2 A and truncated to 2.5 A. A 1.75 A resolution synchrotron data set was used for phase expansion and refinement. An innovation in the dual-space substructure-solution program SHELXD enabled the individual S atoms of the disulfide bonds to be located using the Cu Kalpha data; this resulted in a marked improvement in the phasing compared with the use of super-S atoms. The VT A3 monomer consists of 46 amino acids with three disulfide bridges and has an overall fold resembling the canonical architecture of the alpha- and beta-thionins, a capital letter L. The asymmetric unit consists of two monomers related by a local twofold axis and held together by hydrophobic interactions between the monomer units. One phosphate anion (confirmed by 31P-NMR and MS) is associated with each monomer.
Debreczeni
Judit E
JE
Lehrstuhl für Strukturchemie, Georg-August Universität, Tammannstrasse 4, Göttingen, Germany.
Girmann
Beatrix
B
Zeeck
Axel
A
Krätzner
Ralph
R
Sheldrick
George M
GM
D001665:Binding Sites; D018360:Crystallography, X-Ray / Q000379:methods; D004220:Disulfides / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010710:Phosphates / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D055672:Static Electricity; D017356:Synchrotrons; D028182:Viscum album / Q000737:chemistry
D004220:Disulfides; D010710:Phosphates; D010940:Plant Proteins; C485281:viscotoxin A3, Viscum album; C488038:viscotoxin