Spatiotemporal rescue of memory dysfunction in Drosophila.
We have developed a method for temporal and regional gene expression targeting (TARGET) in Drosophila and show the simultaneous spatial and temporal rescue of a memory defect. The transient expression of the rutabaga-encoded adenylyl cyclase in the mushroom bodies of the adult brain was necessary and sufficient to rescue the rutabaga memory deficit, which rules out a developmental brain defect in the etiology of this deficit and demonstrates an acute role for rutabaga in memory formation in these neurons. The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required.
McGuire
Sean E
SE
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Le
Phuong T
PT
Osborn
Alexander J
AJ
Matsumoto
Kunihiro
K
Davis
Ronald L
RL
D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005838:Genotype; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008568:Memory / Q000502:physiology*; D024521:Mushroom Bodies / Q000502:physiology*; D009473:Neuronal Plasticity; D010641:Phenotype; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C052130:GAL4 protein, S cerevisiae; C055002:GAL80 protein, S cerevisiae; D008164:Luminescent Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila