title: [Construction of tissue engineering skin containing melanocytes].
abstract: OBJECTIVE
  To construct a tissue engineering skin containing melanocytes by employing tissue engineering method.
  
  
  METHODS
  The keratinocytes, dermal fibroblasts and melanocytes were isolated and purified. Then the cells were used to construct a tissue engineering skin containing melanocytes. The location of melanocytes in the tissue engineering skin were detected by Dopa staining, transmission electron microscope (TEM) and S-100 immunohistochemical staining.
  
  
  RESULTS
  Melanocytes can be detected in the basal layer of the constructed tissue engineering skin. The results of TEM showed that the melanocytes were in good conditions.
  
  
  CONCLUSIONS
  The artificial skin containing melanocytes was successfully constructed in vitro and can be used to repair the full-thickness skin defects.
authors.affiliation: Department of Oral Histology & Pathology, Qin Du Stomatological College, Fourth Military Medical University, Xi'an, Shaanxi, P. R. China 710032.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Xin-wen
authors.initials: XW
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D001672:Biocompatible Materials; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D008544:Melanocytes / Q000166:cytology*; D012867:Skin / Q000166:cytology*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials