PTEN gene mutation and high MIB-1 labeling index may contribute to dissemination in patients with glioblastoma.
Dissemination of glioblastomas is often observed, but the underlying mechanism is not well clarified especially from the genetic viewpoints. The present study examined whether PTEN gene mutations and MIB-1 labeling index (LI) correlate with the dissemination in 39 consecutive patients with glioblastomas. Dissemination was defined as leptomenigeal enhancement by magnetic resonance imaging performed in all patients every 2-3 months. We examined PTEN mutations in 26 patients using cDNA-based direct sequencing and MIB-1 LI in 38 patients. Median survival time of the 39 patients was 16.2 months. Dissemination was found in 17 of 39 patients (43.6%). PTEN mutation was significantly associated with dissemination (P=0.0140), and higher MIB-1 LI (> or =35%) resulted in earlier dissemination (P=0.0156). Kaplan-Meier survival plots showed a significantly poorer survival for patients with PTEN mutation (P=0.0012). The results indicate that the evaluation of PTEN mutation and MIB-1 LI are useful to predict dissemination and prognosis of glioblastomas.
Kato
Hideaki
H
Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
Fujimura
Miki
M
Kumabe
Toshihiro
T
Ishioka
Chikashi
C
Kanamaru
Ryunosuke
R
Yoshimoto
Takashi
T
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D019656:Loss of Heterozygosity / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D012189:Retrospective Studies; D016019:Survival Analysis
D019394:Ki-67 Antigen; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human