Enhanced survival of primary murine dopaminergic neurons induced by a partially purified cell lysate fraction from mouse-derived striatal hybrid monoclonal cells.
Lysates of X61, a striatal-derived cell line, and a partially purified preparation from the lysate (UF4) contain a factor(s) capable of increasing the dopamine content of a mesencephalic-derived dopaminergic cell line (MN9D) and of cultures containing primary dopaminergic neurons. Treatment of cultures containing dopaminergic primary neurons grown in the absence of target cells over a 2 week period with X61 lysate or UF4 resulted in an elevation of dopamine levels of the cultures and of media homovanillic acid as well as a 2.0-fold (UF4) to 2.9-fold (X61 lysate) increase in the density of dopaminergic neurons in treated cultures. The results suggest that the activity factor derived from X61 is capable of preventing dopaminergic cell loss which occurs in the absence of dopaminergic target cells of the corpus striatum.
Won
Lisa
L
Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, 947 East 58th Street, Chicago, IL 60637, USA.
Bubula
Nancy
N
Hessefort
Suzanne
S
Gross
Martin
M
Heller
Alfred
A
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003342:Corpus Striatum / Q000502:physiology; D004298:Dopamine / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D006822:Hybrid Cells; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism
D006133:Growth Substances; D004298:Dopamine