Detection of enterococcal surface protein gene (esp) and amplified fragment length polymorphism typing of glycopeptide-resistant Enterococcus faecium during its emergence in a Greek intensive care unit.
The emergence of glycopeptide-resistant Enterococcus faecium (GREF) in a Greek intensive care unit was studied by amplified fragment length polymorphism analysis and esp gene detection. Three GREF clones harboring the esp gene were recovered from 17 out of 21 patients, indicating the dissemination of genetically homogenous and virulent strains of GREF.
Routsi
C
C
Department of Critical Care, Medical School of Athens University, Evangelismos Hospital, Athens, Greece. croutsi@hotmail.com
Platsouka
E
E
Willems
R J L
RJ
Bonten
M J M
MJ
Paniara
O
O
Saroglou
G
G
Roussos
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics*; D016984:Enterococcus faecium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014644:Genetic Variation; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006115:Greece; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D016896:Treatment Outcome
D001426:Bacterial Proteins; D008565:Membrane Proteins; C116707:enterococcal surface protein, esp