title: Dose rate effect on micronuclei induction in cytokinesis blocked human peripheral blood lymphocytes.
abstract: The effect of dose rate on the induction of micronuclei (MN) in human peripheral blood lymphocytes was investigated over a range of dose rates from 0.125 Gy h(-1) to 178.2 Gy h(-1). The response of MN induction was fitted with a linear quadratic model and the alpha and beta coefficients were estimated. It was found that beta values decrease with decreasing dose rate as in the case of chromosomal aberration. At the dose rate of 0.125 Gy h(-1), pure linear response of MN induction was observed. An attempt was made to simulate the calibration curve for the purpose of biological dosimetry at different dose rates and exposure times. The yields when simulated with the exposure time or the dose rate are in agreement with experimental results.
authors.affiliation: RP & AD, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: N N
authors.initials: NN
authors.lastname: Bhat
authors.forename: B S
authors.initials: BS
authors.lastname: Rao
mesh_terms: D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure*; D003198:Computer Simulation; D003571:Cytochalasin B / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure*; D015162:Micronucleus Tests / Q000379:methods*; D008954:Models, Biological; D011829:Radiation Dosage; D018570:Risk Assessment / Q000379:methods
chemical_list: D003571:Cytochalasin B