[Concordance of leishmaniasis cutaneous tests in Tunisia].
A cross sectional study aimed to evaluate the effect of antigenic preparation (Leishmania infantum versus Leishmania major) and dose of leishmania antigens (5 x 10(6) versus 2.5 x 10(6) parasites in the same volume) on the reproducibility of delayed type hypersensitivity leishmania skin test. Results showed that among 34 individuals involved from visceral leishmaniasis endemic area. 26 (76.5%) had a positif Leishmania infantum leishmania (L-L. infantum) test and 27 (79.4%) to Leishmania major leishmania (L-L. major). Mean size of cutaneous reaction was 5.94 +/- 2.86 mm for L-L. infantum and 5.41 +/- 3.23 mm for L-L. major, with a significant positive linear association (p < 10-3). Intra-class correlation coefficient was 0.80 (CI95% = [0.64-0.93]) and concordance Kappa (kappa) was 0.57 (CI95% = [0.40-0.74]). Among 153 individuals from zoonotic cutaneous leishmaniasis. 92.9% revealed a positive test for both types of leishmanin (L-L. major full dose versus L-L. major half dose). Mean size of cutaneous reaction was 12.61 +/- 4.65 mm for the reference test and 11.30 +/- 3.95 mm for diluted one, with a positive linear association (p < 10-3). Intra-class correlation coefficient was 0.78 (IC95% = [0.71-0.84]) and concordance Kappa (kappa) was 0.82 (IC95% = [0.73-0.91]). These results demonstrate a limited effect of leishmania antigenic variation and antigen dose on the reproducibility of delayed type hypersensitivity induced by the leishmanin test.
Ben Alaya Bouafif
N
N
Laboratoire d'Epidémiologie et d'Ecologie Parasitaire, Institut Pasteur de Tunis.
Ben Salah
A
A
Louzir
H
H
Zaâtour
A
A
Bel Haj Hamida
N
N
Mighri
F
F
Gharbi
A
A
Gramiccia
M
M
Gradoni
L
L
Dellagi
K
K
Ben Ismaïl
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D000953:Antigens, Protozoan / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D019353:Endemic Diseases / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000209:etiology; D018314:Leishmania infantum / Q000276:immunology*; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000379:methods* / Q000592:standards; D018709:Statistics, Nonparametric; D014416:Tunisia / Q000453:epidemiology; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology
D000953:Antigens, Protozoan