title: Resistance function of rice lipid transfer protein LTP110.
abstract: Plant lipid transfer proteins (LTPs) are a class of proteins whose functions are still unknown. Some are proposed to have antimicrobial activities. To understand whether LTP110, a rice LTP that we previously identified from rice leaves, plays a role in the protection function against some serious rice pathogens, we investigated the antifungal and antibacterial properties of LTP110. A cDNA sequence, encoding the mature peptide of LTP110, was cloned into the Impact-CN prokaryotic expression system. The purified protein was used for an in vitro inhibition test against rice pathogens, Pyricularia oryzae and Xanthomonas oryzae. The results showed that LTP110 inhibited the germination of Pyricularia oryzae spores, and its inhibitory activity decreased in the presence of a divalent cation. This suggests that the antifungal activity is affected by ions in the media; LTP110 only slightly inhibited the growth of Xanthomonas oryzae. However, the addition of LTP110 to cultured Chinese hamster ovarian cells did not retard growth, suggesting that the toxicity of LTP110 is only restricted to some cell types. Its antimicrobial activity is potentially due to interactions between LTP and microbe-specific structures.
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Biochemistry and Molecular Biology, School of Life Sciences, Fudan University, Shanghai, 200433, PR China. gexiaochun1@yahoo.com.cn
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Ge
authors.forename: Jichao
authors.initials: J
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Chongrong
authors.initials: C
authors.lastname: Sun
authors.forename: Kaiming
authors.initials: K
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001419:Bacteria / Q000472:pathogenicity; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D017931:DNA Primers; D005658:Fungi / Q000472:pathogenicity; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D017931:DNA Primers; D010940:Plant Proteins; C511920:lipid transfer proteins, plant