title: Cytapheresis with a filter for selective removal of CD4+ T cells in experimental autoimmune encephalomyelitis.
abstract: Experimental autoimmune encephalomyelitis (EAE) is a major animal model of human multiple sclerosis (MS). CD4+ T cells are thought to play a pivotal role in the pathogenesis of EAE and MS. In order to investigate the depletion of CD4+ T cells from the systemic circulation as an effective strategy for the treatment of MS, we performed extracorporeal CD4+ T cell adsorption, using a filter to which anti-CD4+ antibody is immobilized as a ligand, in adoptively transferred EAE. Rats treated with CD4+ T cell removal filter (CD4RF) exhibited milder clinical signs of EAE and earlier recovery than those receiving sham treatment. Moreover, the thymic cells from EAE rats treated with CD4RF exhibited a suppressed proliferative response and IFN-gamma production to myelin basic protein. These results suggest that depletion of CD4+ T cells from the systemic circulation by extracorporeal treatment is a potentially useful strategy for treatment of acute phase and relapsing MS.
authors.affiliation: Department of Neurology, Kawatana National Hospital, Nagasaki, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Nakane
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsuo
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Goto
authors.forename: Megumi
authors.initials: M
authors.lastname: Yoshinaga-Matsumoto
authors.forename: Izumi
authors.initials: I
authors.lastname: Ohtsuru
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Ichinose
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Onodera
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Shibuya
mesh_terms: D000208:Acute Disease; D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002460:Cell Line; D016238:Cytapheresis / Q000295:instrumentation / Q000379:methods*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D007371:Interferon-gamma / Q000378:metabolism; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000276:immunology / Q000473:pathology / Q000628:therapy; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D007371:Interferon-gamma