title: VARICOSE, a WD-domain protein, is required for leaf blade development.
abstract: To gain insight into the processes controlling leaf development, we characterized an Arabidopsis mutant, varicose (vcs), with leaf and shoot apical meristem defects. The vcs phenotype is temperature dependent; low temperature growth largely suppressed defects, whereas high growth temperatures resulted in severe leaf and meristem defects. VCS encodes a putative WD-domain containing protein, suggesting a function involving protein-protein interactions. Temperature shift experiments indicated that VCS is required throughout leaf development, but normal secondary vein patterning required low temperature early in leaf development. The low-temperature vcs phenotype is enhanced in axr1-3 vcs double mutants and in vcs mutants grown in the presence of polar auxin transport inhibitors, however, vcs has apparently normal auxin responses. Taken together, these observations suggest a role for VCS in leaf blade formation.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Canada.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Deyholos
authors.forename: G Frank
authors.initials: GF
authors.lastname: Cavaness
authors.forename: Branden
authors.initials: B
authors.lastname: Hall
authors.forename: Ed
authors.initials: E
authors.lastname: King
authors.forename: Jayson
authors.initials: J
authors.lastname: Punwani
authors.forename: Jaimie
authors.initials: J
authors.lastname: Van Norman
authors.forename: Leslie E
authors.initials: LE
authors.lastname: Sieburth
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D018519:Meristem / Q000235:genetics / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D011392:Proline; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; C483689:VARICOSE protein, Arabidopsis; D011392:Proline