The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA.
A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.
Sohlberg
Björn
B
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
Huang
Jianqiang
J
Cohen
Stanley N
SN
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D026723:Polyadenylation; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*
D012329:RNA, Bacterial; D012343:RNA, Transfer; D011062:Polynucleotide Adenylyltransferase; D011117:Polyribonucleotide Nucleotidyltransferase