[Arbitrary primed polymerase chain reaction for the genotypic identification of Streptococcus sanguis group].
OBJECTIVE
To find out an ideal method used in identification of Streptococcus sanguis group (SSG) strains by arbitrary primed polymerase chain reaction (AP-PCR).


METHODS
AP-PCR was used to distinguish SSG strains by designing 25 bp arbitrary primer 5' AAG AGA GGA GCT AGC TCT TCT TGG A 3'.


RESULTS
There were great differences in the main band of DNA polymorphism among SSG species. The similar band can be obtained from the different DNA extractions in the same species.


CONCLUSIONS
AP-PCR may be useful in the identification and classification of SSG species.
Shi
Yu-cai
YC
Department of Dentistry, Yueqing Hospital, Zhejiang 325600, China.
Zhang
Wei-dong
WD
Chen
Hui
H
D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D013298:Streptococcus sanguis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification