Homeostatic proliferation is a barrier to transplantation tolerance.
Despite the ease of inhibiting immune responses by blockade of T-cell costimulation in naive rodent models, it is difficult to suppress those responses in animals with memory cells. Studies demonstrating the importance of alloreactive T-cell deletion during tolerance induction have promoted use of peritransplant T-cell-depleting therapies in clinical trials. But potentially complicating wide-scale, nonspecific T-cell depletion is the finding that extensive T-cell proliferation can occur under conditions of lymphopenia. This process, termed homeostatic proliferation, may induce acquisition of functional memory T cells. Here, using clinically relevant mouse models of peripheral T-cell depletion, we show that residual nondepleted T cells undergo substantial homeostatic expansion. In this setting, costimulatory blockade neither significantly suppresses homeostatic proliferation nor prevents allograft rejection. In addition, T cells that have completed homeostatic proliferation show dominant resistance to tolerance when adoptively transferred into wild-type recipients, consistent with known properties of memory cells in vivo. These findings establish the importance of homeostatic proliferation in clinically relevant settings, demonstrate the barrier that homeostatic proliferation can present to the induction of transplantation tolerance, and have important implications for transplantation protocols that use partial or complete peripheral T-cell depletion.
Wu
Zihao
Z
Department of Medicine, University of Pennsylvania, Philadelphia, Philadelphia 19104, USA.
Bensinger
Steven J
SJ
Zhang
Jidong
J
Chen
Chuangqi
C
Yuan
Xueli
X
Huang
Xiaolun
X
Markmann
James F
JF
Kassaee
Alireza
A
Rosengard
Bruce R
BR
Hancock
Wayne W
WW
Sayegh
Mohamed H
MH
Turka
Laurence A
LA
D000818:Animals; D006706:Homeostasis; D007108:Immune Tolerance; D008212:Lymphocyte Depletion; D051379:Mice; D013045:Species Specificity; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014181:Transplantation Immunology