Photodeposition of silver can result in metal-enhanced fluorescence.
Chemically deposited silver particles are widely used for surface-enhanced Raman scattering (SERS) and more recently for surface-enhanced fluorescence (SEF), also known as metal-enhanced fluorescence (MEF). We now show that metallic silver deposited by laser illumination results in an approximately 7-fold increased intensity of locally bound indocyanine green. The increased intensity is accompanied by a decreased lifetime and increased photostability. These results demonstrate the possibility of photolithographic preparation of surfaces for enhanced fluorescence in microfluidics, medical diagnostics, and other applications.
Geddes
Chris D
CD
University Maryland Baltimore, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Parfenov
Alexandr
A
Lakowicz
Joseph R
JR
D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence / Q000379:methods*; D010777:Photochemistry / Q000379:methods*; D011485:Protein Binding; D011786:Quality Control; D012709:Serum Albumin / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D012709:Serum Albumin; D012834:Silver; D007208:Indocyanine Green