High-resolution 1H NMR spectroscopy in a live mouse subjected to 1.5 Hz magic angle spinning.
It is demonstrated that the resolution of the (1)H NMR metabolite spectrum in a live mouse can be significantly enhanced by an ultraslow magic angle spinning of the animal combined with a modified phase-corrected magic angle turning (PHORMAT) pulse sequence. Proton NMR spectra were measured of the torso and the top part of the belly of a female BALBc mouse in a 2 T field while spinning the animal at a speed of 1.5 Hz. It was found that even in this relatively low field, with PHORMAT an isotropic spectrum is obtained with line widths that are a factor of 4.6 smaller than those obtained in a stationary mouse. It is concluded that in vivo PHORMAT has the potential to significantly increase the utility of (1)H NMR spectroscopy for biochemical and biomedical animal research.
Wind
Robert A
RA
Pacific Northwest National Laboratory, Richland, Washington 99352, USA. robert.wind@pnl.gov
Hu
Jian Zhi
JZ
Rommereim
Donald N
DN
D000818:Animals; D005260:Female; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C