title: Proton high-resolution magic angle spinning NMR analysis of fresh and previously frozen tissue of human prostate.
abstract: The previously observed improvement in spectral resolution of tissue proton NMR with high-resolution magic angle spinning (HRMAS) was speculated to be due largely to freeze-thawing artifacts resulting from tissue storage. In this study, 12 human prostate samples were analyzed on a 14.1T spectrometer at 3 degrees C, with HRMAS rates of 600 and 700 Hz. These samples were measured fresh and after they were frozen for 12-16 hr prior to thawing. The spectral linewidths measured from fresh and previously frozen samples were identical for all metabolites except citrate and acetate. The metabolite intensities of fresh and freeze-thawed samples depend on the quantification procedures used; however, in this experiment the differences of means were <30%. As expected, it was found that tissue storage impacts tissue quality for pathological analysis, and HRMAS conditions alone are not sufficiently destructive to impair pathological evaluation. Furthermore, although storage conditions affect absolute metabolite concentrations in NMR analysis, relative metabolite concentrations are less affected.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Chin-Lee
authors.initials: CL
authors.lastname: Wu
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Taylor
authors.forename: Wenlei
authors.initials: W
authors.lastname: He
authors.forename: Andrea G
authors.initials: AG
authors.lastname: Zepeda
authors.forename: Elkan F
authors.initials: EF
authors.lastname: Halpern
authors.forename: Anthony
authors.initials: A
authors.lastname: Bielecki
authors.forename: R Gilberto
authors.initials: RG
authors.lastname: Gonzalez
authors.forename: Leo L
authors.initials: LL
authors.lastname: Cheng
mesh_terms: D015925:Cryopreservation; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011467:Prostate / Q000737:chemistry*