Proton leak and hydrogen peroxide production in liver mitochondria from energy-restricted rats.
Energy restriction (ER), without malnutrition, is the only environmental intervention that consistently increases maximum life span in laboratory rodents. One theory proposes that a reduction in energy expenditure and reactive oxygen species production is the mechanism responsible for this action of ER. To further test this theory, proton leak, H2O2 production, lipid peroxidation, and protein carbonyls were measured in mitochondria from FBNF1 rats fed either a control or 40% ER diet (onset at 6 mo of age). Liver mitochondria were isolated at 7 and 12 mo of age. Liver weight decreased 25 and 36% at 1 and 6 mo of ER, respectively (P < 0.05). ER resulted in an increase (P < 0.05) in percent total polyunsaturates, n-6 polyunsaturates, and total unsaturates (6 mo only) in mitochondrial lipids. These changes, however, were not associated with significant alterations in mitochondrial function. State 4 respiration and membrane potential were not different (P > 0.05) between groups at either assessment period. Similarly, proton leak kinetics were not different between control and ER animals. Top-down metabolic control analysis and its extension, elasticity analysis, were used at the 6-mo assessment and revealed no difference in control of the oxidative phosphorylation system between control and ER rats. H2O2 production with either succinate or pyruvate/malate substrates was also not different (P > 0.05) between groups at either time point. In conclusion, ER did not alter proton leak or H2O2 production at this age or stage of restriction in liver.
Ramsey
Jon J
JJ
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. jjramsey@ucdavis.edu
Hagopian
Kevork
K
Kenny
Teresa M
TM
Koomson
Edward K
EK
Bevilacqua
Lisa
L
Weindruch
Richard
R
Harper
Mary-Ellen
ME
D000375:Aging / Q000502:physiology; D000821:Animal Feed; D000818:Animals; D031204:Caloric Restriction; D004734:Energy Metabolism / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology; D017494:Proton Pumps / Q000378:metabolism*; D011522:Protons; D051381:Rats; D011919:Rats, Inbred Strains; D017382:Reactive Oxygen Species / Q000378:metabolism
D017494:Proton Pumps; D011522:Protons; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide