Neutralization of enteric coronaviruses with Escherichia coli cells expressing single-chain Fv-autotransporter fusions.
We report here that fusions of single-chain antibodies (scFvs) to the autotransporter beta domain of the IgA protease of Neisseria gonorrhoeae are instrumental in locating virus-neutralizing activity on the cell surface of Escherichia coli. E. coli cells displaying scFvs against the transmissible gastroenteritis coronavirus on their surface blocked in vivo the access of the infectious agent to cultured epithelial cells. This result raises prospects for antiviral strategies aimed at hindering the entry into target cells by bacteria that naturally colonize the same intestinal niches.
Veiga
Esteban
E
Department of Microbial Biotechnology, Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, 28049 Madrid, Spain.
De Lorenzo
Víctor
V
Fernández
Luis Angel
LA
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D009344:Neisseria gonorrhoeae / Q000201:enzymology; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology* / Q000472:pathogenicity
D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D011993:Recombinant Fusion Proteins; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase