Degradation of ferric EDTA by Burkhol cepacia.
EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5-15 microm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4 + (NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH 7.0, 30 degrees C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d-1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.
Fang
Hung-Yuan
HY
Graduate of Engineering Science, Technology School (Doctoral Program), National YunLin University of Science and Technology, Touliu, Yunlin 640, Taiwan. fanghy@es.yuntech.edu.tw
Chen
Shih-Chin
SC
Chen
Szu-Lin
SL
D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004492:Edetic Acid / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D014871:Water Microbiology
D003470:Culture Media; D005290:Ferric Compounds; D000644:Quaternary Ammonium Compounds; D004492:Edetic Acid; C019179:Fe(III)-EDTA