Development of a physical and genetic map of the virulent Wolbachia strain wMelPop.
We report here the construction of a physical and genetic map of the virulent Wolbachia strain, wMelPop. This map was determined by ordering 28 chromosome fragments that resulted from digestion with the restriction endonucleases FseI, ApaI, SmaI, and AscI and were resolved by pulsed-field gel electrophoresis. Southern hybridization was done with 53 Wolbachia-specific genes as probes in order to determine the relative positions of these restriction fragments and use them to serve as markers. Comparison of the resulting map with the whole genome sequence of the closely related benign Wolbachia strain, wMel, shows that the two genomes are largely conserved in gene organization with the exception of a single inversion in the chromosome.
Sun
Ling V
LV
Section of Vector Biology, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Riegler
Markus
M
O'Neill
Scott L
SL
D015139:Blotting, Southern; D002874:Chromosome Mapping; D016680:Genome, Bacterial; D010641:Phenotype; D014774:Virulence / Q000235:genetics; D020577:Wolbachia / Q000235:genetics* / Q000472:pathogenicity