Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry.
The formal reduction potential (Eo') of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (+/-0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant.
Dorman
Scott C
SC
Department of Chemistry, Birmingham Southern College, Birmingham, AL 35254, USA. sdorman@bsc.edu
Harrington
John P
JP
Martin
Melinda S
MS
Johnson
Timothy V
TV
D000818:Animals; D004563:Electrochemistry / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry*; D006736:Horses; D009211:Myoglobin / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods
D006454:Hemoglobins; D009211:Myoglobin