title: Quantification of sideroxylonals in Eucalyptus foliage by high-performance liquid chromatography.
abstract: This paper describes the extraction and quantification of sideroxylonals, a group of formylated phloroglucinol compounds found in the foliage of some eucalypt species. Samples of dry, ground foliage were Soxhlet-extracted with light petroleum spirit:acetone (4:1) and the resultant extract analysed (in the presence of internal standard) by reversed-phase HPLC without further purification. The yield of sideroxylonals was exponential with time and showed an inflection at ca. 4 h of extraction. It is recommended that samples be extracted for 6 h, giving a 92% recovery of the sideroxylonals. The title compounds deteriorate under various conditions, e.g. 10% are lost when foliage is oven-dried at 40 degrees C compared to freeze-drying. Storing samples in mobile phase led to a slow deterioration of sideroxylonals with a 7% loss after 4 days, while 22% of these compounds were lost from dry, ground eucalypt leaf stored at room temperature for 20 months.
authors.affiliation: School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia. ian.wallis@anu.edu.au
authors.forename: Ian R
authors.initials: IR
authors.lastname: Wallis
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Herlt
authors.forename: Bart M
authors.initials: BM
authors.lastname: Eschler
authors.forename: Midori
authors.initials: M
authors.lastname: Takasaki
authors.forename: William J
authors.initials: WJ
authors.lastname: Foley
mesh_terms: D001572:Benzofurans / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005052:Eucalyptus / Q000737:chemistry*; D010696:Phloroglucinol / Q000302:isolation & purification* / Q000031:analogs & derivatives; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D001572:Benzofurans; C118103:sideroxylonal C; D010696:Phloroglucinol