Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells.
We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.
Draper
Jonathan S
JS
Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Smith
Kath
K
Gokhale
Paul
P
Moore
Harry D
HD
Maltby
Edna
E
Johnson
Julie
J
Meisner
Lorraine
L
Zwaka
Thomas P
TP
Thomson
James A
JA
Andrews
Peter W
PW
D000782:Aneuploidy; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008957:Models, Genetic; D013048:Specimen Handling; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors