title: Bone marrow-derived cells do not incorporate into the adult growing vasculature.
abstract: Bone marrow-Derived cells have been proposed to form new vessels or at least incorporate into growing vessels in adult organisms under certain physiological and pathological conditions. We investigated whether bone marrow-Derived cells incorporate into vessels using mouse models of hindlimb ischemia (arteriogenesis and angiogenesis) and tumor growth. C57BL/6 wild-type mice were lethally irradiated and transplanted with bone marrow cells from littermates expressing enhanced green fluorescent protein (GFP). At least 6 weeks after bone marrow transplantation, the animals underwent unilateral femoral artery occlusions with or without pretreatment with vascular endothelial growth factor or were subcutaneously implanted with methylcholanthrene-induced fibrosarcoma (BFS-1) cells. Seven and 21 days after surgery, proximal hindlimb muscles with growing collateral arteries and ischemic gastrocnemius muscles as well as grown tumors and various organs were excised for histological analysis. We failed to colocalize GFP signals with endothelial or smooth muscle cell markers. Occasionally, the use of high-power laser scanning confocal microscopy uncovered false-positive results because of overlap of different fluorescent signals from adjacent cells. Nevertheless, we observed accumulations of GFP-positive cells around growing collateral arteries (3-fold increase versus nonoccluded side, P<0.001) and in ischemic distal hindlimbs. These cells were identified as fibroblasts, pericytes, and primarily leukocytes that stained positive for several growth factors and chemokines. Our findings suggest that in the adult organism, bone marrow-Derived cells do not promote vascular growth by incorporating into vessel walls but may function as supporting cells.
authors.affiliation: Max-Planck-Institute for Clinical & Physiological Research, Bad Nauheim, Germany. t.ziegelhoeffer@kerckhoff.mpg.de
authors.forename: Tibor
authors.initials: T
authors.lastname: Ziegelhoeffer
authors.forename: Borja
authors.initials: B
authors.lastname: Fernandez
authors.forename: Sawa
authors.initials: S
authors.lastname: Kostin
authors.forename: Matthias
authors.initials: M
authors.lastname: Heil
authors.forename: Robert
authors.initials: R
authors.lastname: Voswinckel
authors.forename: Armin
authors.initials: A
authors.lastname: Helisch
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schaper
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D004730:Endothelium, Vascular / Q000166:cytology; D005263:Femoral Artery; D005347:Fibroblasts / Q000166:cytology; D005354:Fibrosarcoma / Q000098:blood supply*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006614:Hindlimb / Q000098:blood supply; D007511:Ischemia / Q000473:pathology; D007962:Leukocytes / Q000166:cytology; D008026:Ligation; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000473:pathology*; D018919:Neovascularization, Physiologic; D009928:Organ Specificity; D020286:Pericytes / Q000166:cytology; D011828:Radiation Chimera
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins