title: Crystallization and preliminary crystallographic studies of the C-terminal domain of human FKBP52.
abstract: FKBP52 is a high-molecular-weight immunophilin belonging to the FKBP (FK506-binding protein) family. FKBP52 is one of several chaperone proteins associated with untransformed steroid receptors in steroid receptor-hsp90 heterocomplexes. Here, the C-terminal domain (amino acids 145-459) has been cloned, overexpressed and purified. Crystals were obtained using the hanging-drop vapour-diffusion technique with ammonium sulfate as precipitant in 0.1 M Tris pH 8.0 solution. Diffraction data to 2.7 A were collected from a selenomethionine-containing crystal belonging to space group C222(1), with unit-cell parameters a = 114.4, b = 143.1, c = 171.2 A, alpha = beta = gamma = 90 degrees. There are three molecules per asymmetric unit.
authors.affiliation: Laboratory of Structural Biology, Tsinghua University and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, People's Republic of China.
authors.forename: Beili
authors.initials: B
authors.lastname: Wu
authors.forename: Pengyun
authors.initials: P
authors.lastname: Li
authors.forename: Cuiling
authors.initials: C
authors.lastname: Shu
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D022021:Tacrolimus Binding Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012645:Selenomethionine; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4