title: Burkholderia pseudomallei: another emerging pathogen in cystic fibrosis.
abstract: BACKGROUND
  Burkholderia pseudomallei is an important cause of acute fulminant pneumonia and septicaemia in tropical regions of northern Australia and south east Asia. Subacute and chronic forms of the disease also occur. There have been three recent reports of adults with cystic fibrosis (CF) who presumably acquired B pseudomallei infection during extended vacations or residence in either Thailand or northern Australia.
  
  
  METHODS
  The clinical course, molecular characteristics, serology and response to treatment are described in four adult CF patients infected with B pseudomallei. Polymerase chain reaction (PCR) based methods were used to confirm B pseudomallei and exclude B cepacia complex. Genotyping was performed using randomly amplified polymorphic DNA (RAPD) PCR and pulsed field gel electrophoresis (PFGE).
  
  
  RESULTS
  Four patients are described with a mean duration of infection of 32 months. All but one patient lived in tropical Queensland. Two patients (with the longest duration of infection) deteriorated clinically and one subsequently died of respiratory failure. Both responded to intravenous treatment specifically targeting B pseudomallei. Another patient suffered two severe episodes of acute bronchopneumonia following acquisition of B pseudomallei. Eradication of the organism was not possible in any of the cases. PFGE of a sample isolate from each patient revealed the strains to be unique and RAPD analysis showed retention of the same strain within an individual over time.
  
  
  CONCLUSIONS
  These findings support a potential pathogenic role for B pseudomallei in CF lung disease, producing both chronic infection and possibly acute bronchopneumonia. Identical isolates are retained over time and are unique, consistent with likely environmental acquisition and not person to person spread. B pseudomallei is emerging as a significant pathogen for patients with CF residing and holidaying in the tropics.
authors.affiliation: Adult Cystic Fibrosis Unit, The Prince Charles Hospital, Brisbane, Queensland, Australia.
authors.forename: M R
authors.initials: MR
authors.lastname: O'Carroll
authors.forename: T J
authors.initials: TJ
authors.lastname: Kidd
authors.forename: C
authors.initials: C
authors.lastname: Coulter
authors.forename: H V
authors.initials: HV
authors.lastname: Smith
authors.forename: B R
authors.initials: BR
authors.lastname: Rose
authors.forename: C
authors.initials: C
authors.lastname: Harbour
authors.forename: S C
authors.initials: SC
authors.lastname: Bell
mesh_terms: D000293:Adolescent; D000328:Adult; D016957:Burkholderia pseudomallei; D021821:Communicable Diseases, Emerging; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004351:Drug Resistance; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000150:complications*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial