A vibrator prevents streaming during close-arterial infusion into the kidney.
Close-arterial infusion of test substances allows one to study the responses of a selected vascular bed without inducing confounding systemic effects. Unfortunately, laminar flow patterns within the artery cause streaming of the injected factor, so that distribution within the target organ is not homogeneous. We describe a reliable method of overcoming these problems. Specifically, we attach a vibrator (i-Vibe egg) to the syringe containing the test substance. We showed that, without vibration, infusion of a solution of Evans blue (0.5% wt/vol) results in uneven distribution of the dye in the kidney. Vibration of the syringe during infusion allows for uniform coloration of the kidney surface. There is also functional improvement of drug distribution during vibration. Renal blood flow was measured during intrarenal infusion of phenylephrine (150 microl, 0.05-0.5 microg). Vibration caused a significant leftward shift in the dose-response curve, i.e., the phenylephrine-induced reduction in renal blood flow was enhanced by vibration. This cheap, simple method for ensuring adequate mixing of intra-arterially infused substances will facilitate not only the study of renal function in the rat but also infusion of test and therapeutic substances into other organs.
Hamza
Shereen M
SM
475 Heritage Medical Research Centre, Univ. of Alberta, Edmonton, Alberta, Canada, T6G 2S2.
Kaufman
Susan
S
D000818:Animals; D004396:Coloring Agents / Q000493:pharmacokinetics; D005070:Evans Blue / Q000493:pharmacokinetics; D007261:Infusions, Intra-Arterial / Q000295:instrumentation / Q000379:methods*; D007668:Kidney / Q000098:blood supply / Q000502:physiology*; D007677:Kidney Function Tests / Q000379:methods*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D012079:Renal Circulation / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014732:Vibration
D004396:Coloring Agents; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D005070:Evans Blue