title: Influence of the fatty acid composition of lipids in chylomicron remnants derived from fish or corn oil on the lipid profile of cultured rat hepatocytes.
abstract: The aim of this work was to characterise the lipid and fatty acid composition of chylomicron remnants enriched in n-3 or n-6 polyunsaturated fatty acids (PUFA) and to investigate their influence on the fatty acid profiles of the lipids of rat hepatocytes cultured in monolayers. Chylomicrons were prepared from the lymph collected from the thoracic duct of rats given an oral dose of fish or corn oil (high in n-3 and n-6 PUFA, respectively), and remnants were prepared in vitro from such chylomicrons using rat plasma containing lipoprotein lipase. The fatty acids predominating in the oils abounded also in their respective chylomicrons and remnants, especially in triacylglycerols. Chylomicrons as well as remnants contained small amounts of phospholipids and long-chain PUFA that were minor in, or absent from, the dietary oils, evidently provided by the intestinal epithelium. The incubation of hepatocytes for 6 h, with either n-3 or n-6 PUFA-rich remnants (0.25-0.75 mM triacylglycerol) resulted in a dose-dependent increase in the amount of triacylglycerols and phospholipids in the cells, which was not affected further by increasing the incubation time to 19 h. Whereas hepatocyte triacylglycerols mostly incorporated the PUFA predominating in each remnant type, the fatty acid profile of cell phospholipids was virtually unchanged. In addition, irrespective of whether they were enriched in n-3 or n-6 PUFA, remnants promoted a relative decrease in the amount of cholesteryl esters, a minor hepatocyte lipid class poor in PUFA. The results demonstrate that the hepatocyte fatty acid profile is modulated in a lipid-class specific way by the amount and type of dietary PUFA delivered to cells in chylomicron remnants.
authors.affiliation: Department of Physiology, University of the Basque Country Medical School, Bilbao, Spain.
authors.forename: E N
authors.initials: EN
authors.lastname: Maldonado
authors.forename: Y
authors.initials: Y
authors.lastname: Chico
authors.forename: K M
authors.initials: KM
authors.lastname: Botham
authors.forename: M I
authors.initials: MI
authors.lastname: Aveldaño
authors.forename: B
authors.initials: B
authors.lastname: Ochoa
mesh_terms: D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D002914:Chylomicrons / Q000494:pharmacology*; D003314:Corn Oil / Q000493:pharmacokinetics*; D015525:Fatty Acids, Omega-3 / Q000493:pharmacokinetics*; D005395:Fish Oils / Q000493:pharmacokinetics*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010743:Phospholipids / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000493:pharmacokinetics
chemical_list: D002914:Chylomicrons; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010743:Phospholipids; D014280:Triglycerides; D003314:Corn Oil