Crystallization and preliminary X-ray diffraction analysis of Mycobacterium smegmatis Dps.
Three crystal forms of a DNA-binding protein from stationary phase cells (Dps) of Mycobacterium smegmatis have been grown. They are hexagonal, tetragonal and cubic. The structure of the cubic form, which has one subunit in the asymmetric unit, has been solved using the molecular-replacement method. The application of the crystallographic symmetry operations on the subunit leads to eight dodecamers with 23 symmetry in the unit cell.
Roy
Siddhartha
S
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Gupta
Surbhi
S
Das
Satyabrata
S
Sekar
K
K
Chatterji
Dipankar
D
Vijayan
M
M
D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000737:chemistry*; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins