Primary antisera against selected steroids or proteins and secondary antisera against gamma-globulins--an available tool for studies of reproductive processes.
This paper presents characteristics of different polyclonal antisera raised against several steroid and protein antigens: 1/ primary antisera against steroid hormones: estradiol-17beta (anti-E2), estrone (anti-E1), testosterone (anti-T), androstendione (anti-A4), cortisol (anti-F) and corticosterone (anti-B); 2/ primary antisera against porcine luteinizing hormone (anti-pLH) and against different forms of porcine pregnancy associated glycoproteins (anti-pPAG) - proteins produced by chorionic tissue; 3/ secondary monovalent antisera raised against rabbit gamma-globulins (Sm-r); 4/ secondary polyvalent antisera against rabbit, pig and quinea pig gamma-globulins mixed at a ratio 1:1:1 (Sp-rpq). All antisera described in the paper present sufficient quality to be routinely used in various RIA, ELISA or Western determinations in physiological and clinical studies of reproductive processes. The antisera against steroid hormones and pLH are available on request.
Szafrańska
Bozena
B
Department of Animal Physiology, University of Warmia and Mazury, Olsztyn, Poland. szafran@uwm.edu.pl
Ziecik
Adam
A
Okrasa
Stanisław
S
D000735:Androstenedione / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D003345:Corticosterone / Q000276:immunology; D004958:Estradiol / Q000276:immunology; D004970:Estrone / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006168:Guinea Pigs; D006728:Hormones / Q000276:immunology*; D006854:Hydrocortisone / Q000276:immunology; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology*; D007114:Immunization; D007986:Luteinizing Hormone / Q000276:immunology; D011257:Pregnancy Proteins / Q000276:immunology; D011817:Rabbits; D012098:Reproduction / Q000502:physiology*; D013552:Swine; D013739:Testosterone / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D006023:Glycoproteins; D006728:Hormones; D007106:Immune Sera; D011257:Pregnancy Proteins; D005719:gamma-Globulins; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D007986:Luteinizing Hormone; D003345:Corticosterone; D006854:Hydrocortisone