A simple and simultaneous determination of acyclovir and ganciclovir in human plasma by high-performance liquid chromatography.
A simple high-performance liquid chromatographic method was developed for the simultaneous determination of the therapeutic levels of acyclovir and ganciclovir in human plasma. After precipitation of plasma proteins with 6% perchloric acid, acyclovir and ganciclovir were simultaneously determined by reversed-phase chromatography with spectophotometric detection at 254 nm. The peak heights for acyclovir and ganciclovir were linearly related to their concentrations ranging from 0.063 to 2.080 micro g/mL. The recovery was 100.48-102.84% for acyclovir and 99.26-103.07% for ganciclovir. The intra- and inter-day relative standard deviation values were in the range 0.186-8.703% for acyclovir and 0.137-6.424% for ganciclovir. The detection limits for both compounds were 0.01 micro g/mL determined as the signal-to-noise ratio of 3. The present method is applicable to therapeutic monitoring during antiviral medication.
Teshima
Daisuke
D
Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan. dteshima@st.hosp.kyushu-u.ac.jp
Otsubo
Kaori
K
Yoshida
Tsuneko
T
Itoh
Yoshinori
Y
Oishi
Ryozo
R
D000212:Acyclovir / Q000097:blood*; D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015774:Ganciclovir / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000998:Antiviral Agents; D015774:Ganciclovir; D000212:Acyclovir