Cytoglobin/STAP, its unique localization in splanchnic fibroblast-like cells and function in organ fibrogenesis.
Cytoglobin/stellate cell activation-associated protein (Cygb/STAP) consists of a new class of hexacoordinate globin superfamily, which was recently discovered by a proteome analysis on the rat hepatic stellate cells. Unlike haemoglobin, myoglobin, and neuroglobin, Cygb/STAP is ubiquitously expressed in several organs, although its detailed localization has not been clarified. Immunohistochemistry and immunoelectron microscopy revealed that Cygb/STAP is uniquely localized in fibroblast-like cells in splanchnic organs, namely the vitamin A-storing cell lineage, but neither in epithelial cells, endothelial cells, muscle cells, blood cells, macrophages, nor dermal fibroblasts. The expression of Cygb/STAP was upregulated in fibrotic lesions of the pancreas and kidney in which activated fibroblast-like cells or myofibroblasts are known to increase in number. In cultured hepatic stellate cells, Cygb/STAP expression was augmented by the stimulation with sera, platelet-derived growth factor-BB, and transforming growth factor-beta 1. Overexpression of Cygb/STAP in NIH 3T3 cells induced the cells to lessen migratory activities and increase the expression of collagen alpha1(I) mRNA. These results indicate that Cygb/STAP is a tissue globin uniquely localized in splanchnic fibroblastic cell lineage and may play a role in fibrotic organ disorder.
Nakatani
Kazuki
K
Department of Anatomy, Graduate School of Medicine, Osaka City University, Japan.
Okuyama
Hiroaki
H
Shimahara
Yasuyuki
Y
Saeki
Shigeru
S
Kim
Dong-Ho
DH
Nakajima
Yuji
Y
Seki
Shuichi
S
Kawada
Norifumi
N
Yoshizato
Katsutoshi
K
D000818:Animals; D000077214:Becaplermin; D015415:Biomarkers / Q000032:analysis; D002908:Chronic Disease; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000078842:Cytoglobin; D004195:Disease Models, Animal; D005355:Fibrosis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D005914:Globins / Q000032:analysis / Q000378:metabolism*; D006420:Hemeproteins / Q000032:analysis / Q000378:metabolism*; D007674:Kidney Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D007728:Kupffer Cells / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D010179:Pancreas / Q000201:enzymology / Q000473:pathology; D010195:Pancreatitis / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D014801:Vitamin A / Q000032:analysis / Q000378:metabolism
D015415:Biomarkers; D024042:Collagen Type I; C000627268:Cygb protein, rat; D000078842:Cytoglobin; D006420:Hemeproteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; C508961:Tgfb1 protein, mouse; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014801:Vitamin A; D000077214:Becaplermin; D005914:Globins; D010544:Peroxidases