Rapid analysis of the Vbeta repertoire of CD4 and CD8 T lymphocytes in whole blood.
Determination of the T cell receptor Vbeta repertoire of human CD4 and CD8 populations is a useful immunological tool, particularly in the investigation of superantigen involvement in various disease states. We describe the optimisation of a rapid technique for the simultaneous evaluation of 24 Vbeta families of the T cell receptor of CD4 and CD8 positive lymphocytes in whole blood by flow cytometry adapting a commercially available monoclonal antibody kit. The technique described is reliable and reproducible, and we describe its use as a potential diagnostic tool in patients with staphylococcal and streptococcal toxic shock syndromes.
MacIsaac
Christopher
C
Intensive Care Unit, The Royal Melbourne Hospital, Australia.
Curtis
Nigel
N
Cade
John
J
Visvanathan
Kumar
K
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D002648:Child; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D012772:Shock, Septic / Q000175:diagnosis
D011933:Reagent Kits, Diagnostic; D016693:Receptors, Antigen, T-Cell, alpha-beta