title: Functional expression of the short isoform of the murine leptin receptor Ob-Rc (muB1.219) in Xenopus laevis oocytes.
abstract: Leptin, a hormone mainly secreted by the adipose tissue, acts on the hypothalamus to regulate food intake and thermogenesis. Six leptin receptor isoforms have been identified and localized in different tissues. While it is clear that leptin action in the brain occurs by binding to the long receptor isoform, several studies have shown that the short isoforms could be involved in the transcellular transport of the hormone from the blood to the brain. Based on these works, we decided to investigate whether the murine short leptin receptor isoform Ob-Rc (muB1.219) could transport leptin when expressed in Xenopus laevis oocytes. MuB1.219 cRNA was injected into the oocytes and functional studies were performed by incubating the oocytes in the presence of 2.5 nM [125I]-leptin, under different conditions. Results showed that leptin binding to the injected oocytes was four to eight-fold higher than the binding to the non-injected oocytes. This was blocked by 250 nM of non-radiolabeled leptin, suggesting that the binding was specific. Leptin internalization was observed from 30 min incubation onwards. Coexpression of the human Na+/glucose cotransporter and the leptin receptor showed that leptin increased sugar uptake into the oocytes. These results demonstrate that the short leptin receptor Ob-Rc is able to mediate binding and internalization of the hormone when expressed in oocytes and that it may perform intracellular signaling.
authors.affiliation: Departamento de Fisiología y Nutrición, Universidad de Navarra, 31080 Pamplona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Barrenetxe
authors.forename: R
authors.initials: R
authors.lastname: Palacios
authors.forename: A
authors.initials: A
authors.lastname: Barber
authors.forename: M P
authors.initials: MP
authors.lastname: Lostao
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D050260:Carbohydrate Metabolism; D015870:Gene Expression; D006801:Humans; D007536:Isomerism; D020738:Leptin / Q000378:metabolism / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D054411:Receptors, Leptin; D051269:Sodium-Glucose Transporter 1; D014982:Xenopus laevis
chemical_list: C515448:LEPR protein, human; D020738:Leptin; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; C515447:leptin receptor, mouse