title: High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis.
abstract: BACKGROUND
  Japanese cedar pollinosis is the most common form of hayfever in Japan in spring and has remarkably increased since 1960.
  
  
  OBJECTIVE
  We sought to clarify the candidate genes for cedar pollinosis using a case-control study.
  
  
  METHODS
  After diagnosing 351 subjects on the basis of an intradermal test, nasal provocation test, and questionnaire regarding nasal and conjunctival symptoms, we determined the blood-specific IgE values and genotypes of eosinophil peroxidase (EPO) and interleukin-4 receptor alpha-chain (IL4RA) in 145 patients with pollinosis and 206 healthy subjects, including 75 healthy subjects with higher specific IgE values.
  
  
  RESULTS
  We found significant differences in the frequencies of Pro358Leu in EPO and of Ile50Val and Glu375Ala in IL4RA between patients and healthy subjects. There was a significantly higher frequency of 358Leu in EPO in patients than in healthy subjects showing a higher specific IgE value. In contrast, we recognized significant changes in the prevalence of Ile50Val and Glu375Ala in IL4RA in healthy subjects with a normal IgE value compared with those in healthy subjects with a higher specific IgE value. The relationship between EPO polymorphisms and the onset of symptoms was exactly opposite that for IL4RA.
  
  
  CONCLUSIONS
  These results suggest that Pro358Leu in EPO is strongly involved in the development of cedar pollinosis. Ile50Val and Glu375Ala in IL4RA seem to be related to cedar pollen sensitization. Subjects with Ile50 or Glu375 might develop cedar pollinosis with increased exposure to cedar pollen.
authors.affiliation: Department of Environmental Medicine, Kochi Medical School, Nankoku, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Miyagawa
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Endo
authors.forename: Toru
authors.initials: T
authors.lastname: Imai
authors.forename: Kotaro
authors.initials: K
authors.lastname: Ozasa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Motohashi
authors.forename: Ichiyo
authors.initials: I
authors.lastname: Matsuzaki
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sasahara
authors.forename: Kotaro
authors.initials: K
authors.lastname: Hatta
authors.forename: Akira
authors.initials: A
authors.lastname: Eboshida
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D016022:Case-Control Studies; D032485:Cryptomeria; D047209:Eosinophil Peroxidase; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007428:Intradermal Tests; D008297:Male; D008875:Middle Aged; D009299:Nasal Provocation Tests; D010544:Peroxidases / Q000235:genetics*; D011058:Pollen / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D019948:Receptors, Interleukin-4 / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D011795:Surveys and Questionnaires
chemical_list: D000485:Allergens; D019948:Receptors, Interleukin-4; D007073:Immunoglobulin E; D047209:Eosinophil Peroxidase; D010544:Peroxidases