title: The beta3 nicotinic receptor subunit: a component of alpha-conotoxin MII-binding nicotinic acetylcholine receptors that modulate dopamine release and related behaviors.
abstract: Nigrostriatal dopaminergic neurons express many nicotinic acetylcholine receptor (nAChR) subunits capable of forming multiple nAChR subtypes. These subtypes are expressed differentially along the neuron and presumably mediate diverse responses. beta3 subunit mRNA has restricted expression but is abundant in the substantia nigra and ventral tegmental areas. To investigate the potential role(s) of nicotinic receptors containing the beta3 subunit in dopaminergic tracts, we generated mice with a null mutation in the beta3 gene. We were thereby able to identify a population of beta3-dependent alpha-conotoxin MII-binding nAChRs that modulate striatal dopamine release. Changes were also observed in locomotor activity and prepulse inhibition of acoustic startle, behaviors that are controlled, in part, by nigrostriatal and mesolimbic dopaminergic activity, respectively, suggesting that beta3-containing nAChRs modulate these behaviors.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California 92037-1099, USA.
authors.forename: Changhai
authors.initials: C
authors.lastname: Cui
authors.forename: T K
authors.initials: TK
authors.lastname: Booker
authors.forename: Roberta S
authors.initials: RS
authors.lastname: Allen
authors.forename: Sharon R
authors.initials: SR
authors.lastname: Grady
authors.forename: Paul
authors.initials: P
authors.lastname: Whiteaker
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Marks
authors.forename: Outi
authors.initials: O
authors.lastname: Salminen
authors.forename: Theresa
authors.initials: T
authors.lastname: Tritto
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Butt
authors.forename: W R
authors.initials: WR
authors.lastname: Allen
authors.forename: Jerry A
authors.initials: JA
authors.lastname: Stitzel
authors.forename: J Michael
authors.initials: JM
authors.lastname: McIntosh
authors.forename: Jim
authors.initials: J
authors.lastname: Boulter
authors.forename: Allan C
authors.initials: AC
authors.lastname: Collins
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Heinemann
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001667:Binding, Competitive; D020916:Conotoxins / Q000378:metabolism*; D003342:Corpus Striatum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D018390:Gene Targeting; D017403:In Situ Hybridization; D008024:Ligands; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D009043:Motor Activity / Q000235:genetics; D009433:Neural Inhibition / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism*; D013216:Reflex, Startle / Q000235:genetics; D013378:Substantia Nigra / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
chemical_list: D020916:Conotoxins; D008024:Ligands; D021122:Protein Subunits; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; C098872:alpha-conotoxin MII; D009538:Nicotine; D004298:Dopamine