Sucrose increases calcium-dependent protein kinase and phosphatase activities in potato plants.
The effect of sucrose on tuber formation, calcium-dependent protein kinase (CDPK) and phosphatase activities was analysed using in vitro cultured potato plants. In short treatments, sucrose induced CDPK and phosphatase activities. In long treatments, sucrose induced tuber formation in the absence of other tuber inducing stimuli. Sorbitol caused a minor increase in CDPK activity and affected plant morphology but did not induce tuber development. The addition of the protein kinase inhibitor Staurosporine precluded sucrose-induced tuberization. Altogether, our results suggest that phosphorylation/dephosphorylation events are involved in sucrose-induced tuber development.
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Instituto de Investigaciones en Ingeniería Genética y Biología Molecular and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina.
MacIntosh
G C
GC
Ulloa
R M
RM
Gargantini
P R
PR
Vozza
N F
NF
Téllez-Inón
M T
MT
D002118:Calcium / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011198:Solanum tuberosum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D002118:Calcium; D010744:Phosphoric Monoester Hydrolases; D011494:Protein Kinases; D013395:Sucrose; D017871:Calcium-Calmodulin-Dependent Protein Kinases