title: Typing of Mycobacterium tuberculosis strains resistant to rifampicin and isoniazid by molecular biological methods.
abstract: Mutations in the rpoB, katG, inhA, oxyR/ahpC genes in rifampicin- and isoniazid-resistant M. tuberculosis strains isolated from residents of Moscow, Astrakhan', and Moldova Republic were studied by molecular biological methods (heteroduplex analysis, single strand conformational polymorphism, biochips). Twenty-five combinations of mutations were detected. Some differences in the type distribution of detected mutations were found. The use of biochips is the most perspective method for determining the type of mutation.
authors.affiliation: Moscow Tuberculosis Control Center, Moscow Public Health Committee.
authors.forename: O I
authors.initials: OI
authors.lastname: Skotnikova
authors.forename: E Yu
authors.initials: EY
authors.lastname: Nosova
authors.forename: O V
authors.initials: OV
authors.lastname: Markova
authors.forename: O A
authors.initials: OA
authors.lastname: Irtuganova
authors.forename: V N
authors.initials: VN
authors.lastname: Krudu
authors.forename: S S
authors.initials: SS
authors.lastname: Ginda
authors.forename: O V
authors.initials: OV
authors.lastname: Golishcheva
authors.forename: M N
authors.initials: MN
authors.lastname: Biryukova
authors.forename: E N
authors.initials: EN
authors.lastname: Strel'tsova
authors.forename: O V
authors.initials: OV
authors.lastname: Kalyanina
authors.forename: V I
authors.initials: VI
authors.lastname: Litvinov
authors.forename: A M
authors.initials: AM
authors.lastname: Moroz
authors.forename: S A
authors.initials: SA
authors.lastname: Lapa
authors.forename: D A
authors.initials: DA
authors.lastname: Gryadunov
authors.forename: V M
authors.initials: VM
authors.lastname: Mikhailovich
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003062:Codon; D004247:DNA / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes; D020411:Oligonucleotide Array Sequence Analysis; D018807:Polymorphism, Single-Stranded Conformational; D012293:Rifampin / Q000494:pharmacology*; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D000995:Antitubercular Agents; D003062:Codon; D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D007538:Isoniazid; D012293:Rifampin