title: Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses.
abstract: To identify cold-, drought-, high-salinity-, and/or abscisic acid (ABA)-inducible genes in rice (Oryza sativa), we prepared a rice cDNA microarray including about 1700 independent cDNAs derived from cDNA libraries prepared from drought-, cold-, and high-salinity-treated rice plants. We confirmed stress-inducible expression of the candidate genes selected by microarray analysis using RNA gel-blot analysis and finally identified a total of 73 genes as stress inducible including 58 novel unreported genes in rice. Among them, 36, 62, 57, and 43 genes were induced by cold, drought, high salinity, and ABA, respectively. We observed a strong association in the expression of stress-responsive genes and found 15 genes that responded to all four treatments. Venn diagram analysis revealed greater cross talk between signaling pathways for drought, ABA, and high-salinity stresses than between signaling pathways for cold and ABA stresses or cold and high-salinity stresses in rice. The rice genome database search enabled us not only to identify possible known cis-acting elements in the promoter regions of several stress-inducible genes but also to expect the existence of novel cis-acting elements involved in stress-responsive gene expression in rice stress-inducible promoters. Comparative analysis of Arabidopsis and rice showed that among the 73 stress-inducible rice genes, 51 already have been reported in Arabidopsis with similar function or gene name. Transcriptome analysis revealed novel stress-inducible genes, suggesting some differences between Arabidopsis and rice in their response to stress.
authors.affiliation: Biological Resources Division, Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
authors.forename: M Ashiq
authors.initials: MA
authors.lastname: Rabbani
authors.forename: Kyonoshin
authors.initials: K
authors.lastname: Maruyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: M Ayub
authors.initials: MA
authors.lastname: Khan
authors.forename: Koji
authors.initials: K
authors.lastname: Katsura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ito
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yoshiwara
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000064:Acclimatization / Q000235:genetics*; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004190:Disasters; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D015723:Gene Library; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D009994:Osmolar Concentration; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D012965:Sodium Chloride; D000040:Abscisic Acid