Detection and description of various stores of nitric oxide store in vascular wall.
We analyzed the possibility of the existence of various NO pools in the vascular wall. Incubation of isolated rat aorta with dinitrosyl iron complex (NO donor) led to the formation of NO stores in the vascular wall detected by vascular relaxation response induced by diethyldithiocarbamate and N-acetylcysteine. Comparison of the effects of successive application of diethyldithiocarbamate and N-acetylcysteine revealed two NO pools (one pool responded to both agents, while other responded only to N-acetylcysteine). Inhibition of guanylate cyclase with methylene blue abolished the response to diethyldithiocarbamate, while the reaction to N-acetylcysteine decreased by the value, corresponding to diethyldithiocarbamate-dependent relaxation. It is hypothesized that in the vascular wall NO is stored in the form protein-bound dinitrosyl iron complexes and S-nitrosothiols in hydrophilic and hydrophobic cell compartments.
Vlasova
M A
MA
Institute of General Pathology and Pathological Physiology, Russian Academy of Medical Sciences, Moscow.
Vanin
A F
AF
Muller
B
B
Smirin
B V
BV
Malyshev
I Yu
IY
Manukhina
E B
EB
D000111:Acetylcysteine / Q000737:chemistry; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D004050:Ditiocarb / Q000737:chemistry; D004730:Endothelium, Vascular / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology; D007501:Iron / Q000378:metabolism*; D008297:Male; D008956:Models, Chemical; D009119:Muscle Contraction / Q000187:drug effects; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D026403:S-Nitrosothiols / Q000737:chemistry; D013997:Time Factors
D000276:Adjuvants, Immunologic; D016166:Free Radical Scavengers; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D026403:S-Nitrosothiols; D009569:Nitric Oxide; C073661:dinitrosyl iron complex; D004050:Ditiocarb; D007501:Iron; D000111:Acetylcysteine