[Experimental study of mouse infected with human cytomegalovirus AD169 strain].
OBJECTIVE
To determine the sensitivity of mouse to human cytomegalovirus AD169 strain (HCMV AD169) infection and to establish a stable animal model for developmental disorders.


METHODS
Forty kunmin mice were divided into two groups randomly: the virus inoculated group and the control group. After 7, 15, 30, and 60 days, the mice's brain development was observed by pathological techniques. HCMV antigen was detected by immunohistochemical method and HCMV DNA was analyzed by polymerase chain reaction (PCR).


RESULTS
The extensive pathological damages and developmental disorders in the brain tissues of infected mice were observed. Meanwhile, the viral antigen and viral DNA were also demonstrated in the brain tissues of HCMV infected mice.


CONCLUSIONS
HCMV can cause brain developmental disorders in mice, which provides a rationale for understanding the mechanism of congenital abnormality by HCMV infection.
Xie
Ni
N
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Chen
Li-yu
LY
Dai
Gan
G
D000818:Animals; D000831:Animals, Newborn; D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections / Q000150:complications / Q000473:pathology; D004195:Disease Models, Animal; D051379:Mice; D009421:Nervous System Malformations / Q000821:virology; D011897:Random Allocation