Analysis of genes expressed in head kidney of common carp Cyprinus carpio L treated with cortisol.
We analyzed genes expressed from head kidney of common carp Cyprinus carpio L. treated with cortisol. The results of single-pass sequencing of expressed sequence tags (ESTs) from 188 clones (AU240288-AU240367, AU301120-AU301227) from kidney cDNA are presented. One-hundred-twenty-seven clones (67.6%) were completely unknown and are likely to represent newly described genes, whereas 61 clones (32.4%) were identified based on matches to sequences in the database. The putative genes contain several ribosomal proteins, cytochrome oxidase subunits. Immune related cDNA clones identified from kidney were immunoglobulin light chain (n=4), FK506/rapamycin-binding protein (FKBP), CXC chemokine receptor type 4, complement factor B/C2-A3, peptidylprolyl isomerase (cyclophilin; Cyp)-like1, cyclophilin S1, heat shock-70 kDa protein-4, stress-activated protein kinase-3 (n=2). FKBP and cyclophilin genes expressed in normal tissues (head kidney, spleen, liver, brain and heart). Expression of FKBP and cyclophilin genes were not detected in liver, brain and heart when treated with cortisol for 16 h.
Kawano
Haruhiko
H
Faculty of Agriculture, Miyazaki University, Gakuen kibanadai-nishi 1-1, Miyazaki 889-2192, Japan.
Kono
Tomoya
T
Watanuki
Hironobu
H
Savan
Ram
R
Sakai
Masahiro
M
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002347:Carps / Q000235:genetics*; D021983:Cyclophilins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000235:genetics
D018076:DNA, Complementary; D013481:Superoxides; D021983:Cyclophilins; D022021:Tacrolimus Binding Proteins; D006854:Hydrocortisone