title: Pits and fissures: relative space contribution in fissures from sealants, prophylaxis pastes and organic remnants.
abstract: BACKGROUND
  Previous studies by the authors have looked at the nature of the fissure system of human permanent molars and premolars, and has provided evidence for the presence of a prismless layer of enamel. It was noted during these studies that the fissure spaces were often occupied by material other than the fissure sealant. The aim of this study was to define these materials and to look at the percentage contribution of each to the sealed fissure space.
  
  
  METHODS
  A sample of teeth, both molars and premolars, were sealed with an unfilled fissure sealant after prophylaxis with a coloured prophylaxis paste. In one group, the crown of the tooth was removed by dissolution in hydrochloric acid following placement of the sealant. This revealed a negative image of the fissure system and its contents. The second group of teeth was sectioned following sealing, and the contents of the fissure space were analyzed.
  
  
  RESULTS
  The negative image of the fissure system displayed the fissure contents by colour and the sectioned teeth were able to be computer analyzed to establish the relative contribution of sealant, prophylaxis paste and organic material to the fissure space.
  
  
  CONCLUSIONS
  Sealant contribution was in the range of 14-96 per cent, prophylaxis paste from 0-50 per cent and organic remnants 0-55 per cent. The presence of these last two components could contribute to sealant loss.
authors.affiliation: Dental School, The University of Adelaide, South Australia. jburrow@chariot.net.au
authors.forename: J F
authors.initials: JF
authors.lastname: Burrow
authors.forename: M F
authors.initials: MF
authors.lastname: Burrow
authors.forename: O F
authors.initials: OF
authors.lastname: Makinson
mesh_terms: D001641:Bicuspid / Q000648:ultrastructure; D003116:Color; D003743:Dental Enamel / Q000648:ultrastructure*; D003777:Dental Prophylaxis / Q000295:instrumentation; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D016628:Replica Techniques; D017640:Silicates / Q000737:chemistry; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry; D014100:Toothpastes / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry
chemical_list: D009930:Organic Chemicals; D010895:Pit and Fissure Sealants; D017640:Silicates; D017971:Tin Compounds; D014100:Toothpastes; C003784:zircon; D015040:Zirconium; C045358:stannic oxide