Cloning and characterization of GITR ligand.
The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-kappaB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+CD25+ regulatory T-cell-mediated suppressive activities.
Kim
J D
JD
Immunomodulation Research Center, University of Ulsan, Ulsan, Korea.
Choi
B K
BK
Bae
J S
JS
Lee
U H
UH
Han
I S
IS
Lee
H W
HW
Youn
B S
BS
Vinay
D S
DS
Kwon
B S
BS
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism; D005434:Flow Cytometry; D040461:Gene Components; D015870:Gene Expression; D053450:Glucocorticoid-Induced TNFR-Related Protein; D006801:Humans; D008024:Ligands; D008156:Luciferases; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013491:Suppressor Factors, Immunologic / Q000037:antagonists & inhibitors; D048069:Tumor Necrosis Factors
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D053450:Glucocorticoid-Induced TNFR-Related Protein; D008024:Ligands; D016328:NF-kappa B; D017475:Receptors, Nerve Growth Factor; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; C491405:TNFRSF18 protein, human; C106544:Tnfrsf18 protein, mouse; C478966:Tnfsf18 protein, mouse; D048069:Tumor Necrosis Factors; D008156:Luciferases