Identification of 6-aminochrysene photoproducts and study of the effect of a humic acid and riboflavin on its photolysis.
The effect of the photosensitizer riboflavin (0, 10, 25, 50, 100 microM) and a river humic acid (0, 20, 40, 80 ppm) on the photolysis of 6-aminochrysene (6AC) in 10 mM PBS solution (pH 7.0) was studied. The presence of riboflavin significantly enhanced photolysis rate of 6AC. The photo-transformation half-life of 6AC was 1 or 36 min, respectively, in the presence or absence of riboflavin. The humic acid inhibited the photo-transformation rate of 6AC. The photo-transformation half-lives of 6AC were 37, 56, 92 min at 20, 40, 80 ppm humic acid, respectively. By using LC-MS, the main 6AC photoproduct identified was 5,6-chrysene-quinone along with some minor products. Both 6AC and 5,6-chrysene-quinone exhibited photoinduced cytotoxicity. A photochemical transformation pathway for 6AC was derived.
Zeng
Kui
K
Department of Biology, Jackson State University, Jackson, MS 39217, USA.
Hwang
Huey-min
HM
Zhang
Yi
Y
Yu
Hongtao
H
D002911:Chrysenes / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects*; D006812:Humic Substances; D010782:Photolysis / Q000187:drug effects* / Q000528:radiation effects*; D012256:Riboflavin / Q000494:pharmacology*
D002911:Chrysenes; D006812:Humic Substances; C100101:6-chrysenamine; D012256:Riboflavin