Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targets.
Biofilms are highly ordered microbial communities enmeshed in a carefully sculpted matrix designed for survival of organisms either in multi- or mono-genus/species in a specific microniche. In human disease, biofilm infections are some of the most recalcitrant to treat. Even with rigorous antibiotic regimens, some biofilms, such as those within the thick airway mucus of cystic fibrosis (CF) patients, persist throughout the course of the disease process. In this editorial, discussion will cover the utility of using advanced proteomic techniques to help identify potential weaknesses in the already impressive defensive armamentarium of biofilm bacteria. Two biofilm systems will be discussed herein, one of which is that of Pseudomonas aeruginosa biofilms within CF airway biofilms. The other is referred to as persistent 'bioterrorist agent biofilms' in which Francisella tularensis can grow on surfaces where environmental amoeba can phagocytose them, allowing for growth of F. tularensis within the amoebae.
Hassett
Daniel J
DJ
Limbach
Patrick A
PA
Hennigan
Robert F
RF
Klose
Karl E
KE
Hancock
Robert E W
RE
Platt
Mark D
MD
Hunt
Donald F
DF
D000048:Acanthamoeba / Q000378:metabolism; D000818:Animals; D018441:Biofilms; D023482:Bioterrorism / Q000517:prevention & control*; D005604:Francisella tularensis / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control; D006801:Humans; D013058:Mass Spectrometry; D008954:Models, Biological; D040901:Proteomics / Q000379:methods*; D011552:Pseudomonas Infections / Q000517:prevention & control; D014612:Vaccines
D014612:Vaccines