title: Production of polyacrylamide gradient gel for lipoprotein electrophoretic separation.
abstract: BACKGROUND
  Small LDL are associated with risk of coronary heart disease. Gradient gel electrophoresis for LDL separation is not a simple method and high-quality non-denaturing gradient gels are lacking.
  
  
  METHODS
  In this paper, we describe a method for the preparation of a polyacrylamide gel system that consists of an upper linear gradient gel (1.8-10%) and a lower homogeneous gel (16%) for the determination of LDL size.
  
  
  RESULTS
  The linear gradient is highly reproducible. Intra-inter gel coefficients of variation for LDL particle size are lower than 0.6%.
  
  
  CONCLUSIONS
  Effective LDL size measurement from pre-stained serum samples is possible in a stable gel.
authors.affiliation: Department of Clinical, Morphologic and Technological Sciences, Clinica Medica, University of Trieste, Cattinara Hospital, Strada di Fiume, 34149 Trieste, Italy. fondam@units.it
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fonda
authors.forename: Anna M
authors.initials: AM
authors.lastname: Semolic
authors.forename: Maria R
authors.initials: MR
authors.lastname: Soranzo
authors.forename: Luigi
authors.initials: L
authors.lastname: Cattin
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D004586:Electrophoresis / Q000295:instrumentation*; D006801:Humans; D016014:Linear Models; D017270:Lipoprotein(a) / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D015203:Reproducibility of Results
chemical_list: D000180:Acrylic Resins; D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; C016680:polyacrylamide gels