Protective effect of D-003 on experimental spinal cord ischemia in rabbits.
D-003 is a natural mixture of long chain aliphatic acids isolated and purified from sugar cane wax. It possesses antiplatelet and antithrombotic effects as well as decreases plasma and serum levels of thromboxane B(2) (TxB(2)), meanwhile significantly and markedly raises prostacyclin (PgI(2)) levels in rats. This study was undertaken to investigate the effects of D-003 on spinal cord injury in rabbits. New Zealand rabbits were treated during 10 days with D-003 (25 and 200 mg kg(-1)) and ASA (2 mg kg(-1)) before spinal cord ischemia. Animals were subjected to 20 min of aortic occlusion and 24h of reperfusion. Clinical symptoms and histopathological changes of spinal cord were observed. The PgI(2) levels in thoracic aorta were quantified by bioassay. D-003 (25 and 200 mg kg(-1)) significantly increased the mean scores reached 4h after reperfusion, although no dose relation was observed. Twenty-four hours after reperfusion, no deaths occurred in both sham and D-003 treated groups, meanwhile in positive controls and ASA the mortality rate was 38.5% and 7.69% respectively. In addition, 100% of sham, 69% and 77% of rabbits treated with D-003 at 25 and 200 mg kg(-1), respectively, did not show histopathological changes. By the contrary, 100% of positive control animals showed severe damage and ASA-treated rabbits showed only a partial protection. Animals treated with both doses of D-003 showed PgI(2) levels significantly larger than those of positive and negative controls, an effect dose-related, while ASA 2 mg kg(-1) did not change PgI(2) values. The increase of PgI(2) levels achieved in the D-003 treated animals could be an important mechanism in the protection against the spinal cord ischemia.
Carbajal
D
D
Department of Pharmacology, Center of Natural Products, National Center for Scientific Research, Ave 25 and 158 P.O. Box 6880, Cubanacan, Havana, Cuba. dalmer@ip.etecsa.cu
Arruzazabala
M L
ML
Noa
M
M
Molina
V
V
Más
R
R
Arango
E
E
Valdés
S
S
Gonzalez
J E
JE
D000818:Animals; D001011:Aorta / Q000187:drug effects; D011464:Epoprostenol / Q000097:blood; D005227:Fatty Acids / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology*; D011817:Rabbits; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology; D020760:Spinal Cord Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control*; D015996:Survival Rate
C418063:D-003 sugarcane wax acid mixture; D005227:Fatty Acids; D020011:Protective Agents; D011464:Epoprostenol