title: Definition of the consensus motif recognized by gamma-adaptin ear domains.
abstract: The heterotetrameric adaptor complex 1 (AP-1) and the monomeric Golgi-localized, gamma ear-containing, Arf-binding (GGA) proteins are components of clathrin coats associated with the trans-Golgi network and endosomes. The carboxyl-terminal ear domains (or gamma-adaptin ear (GAE) domains) of two gamma-adaptin subunit isoforms of AP-1 and of the GGAs are structurally similar and bind to a common set of accessory proteins. In this study, we have systematically defined a core tetrapeptide motif PsiG(P/D/E)(Psi/L/M) (where Psi is an aromatic residue), which is responsible for the interactions of accessory proteins with GAE domains. The definition of this motif has allowed us to identify novel GAE-binding partners named NECAP and aftiphilin, which also contain clathrin-binding motifs. These findings shed light on the mechanism of accessory protein recruitment to trans-Golgi network and endosomal clathrin coats.
authors.affiliation: Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Mattera
authors.forename: Brigitte
authors.initials: B
authors.lastname: Ritter
authors.forename: Sachdev S
authors.initials: SS
authors.lastname: Sidhu
authors.forename: Peter S
authors.initials: PS
authors.lastname: McPherson
authors.forename: Juan S
authors.initials: JS
authors.lastname: Bonifacino
mesh_terms: D033961:Adaptor Protein Complex 1; D033982:Adaptor Protein Complex gamma Subunits / Q000737:chemistry* / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019921:Biotinylation; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D016384:Consensus Sequence; D005982:Glutathione Transferase / Q000235:genetics; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship; D020798:Two-Hybrid System Techniques
chemical_list: D033961:Adaptor Protein Complex 1; D033982:Adaptor Protein Complex gamma Subunits; D033942:Adaptor Proteins, Vesicular Transport; C475910:CLINT1 protein, human; D002352:Carrier Proteins; D002966:Clathrin; D008164:Luminescent Proteins; D010446:Peptide Fragments; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; C121510:SYNRG protein, human; D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase