Frozen-solution conformational analysis by REDOR spectroscopy.
Frozen-solution conformational analysis (FrSCA) can be performed on organic compounds using REDOR spectroscopy. REDOR measurements on frozen aqueous solutions of 13C-methyl beta-15N-aminoglucoside indicate a bimodal distribution of conformations in a 68:32 ratio, with 13C-15N distances of 4.31 and 3.55 A, respectively. The high resolution and straightforward sample preparation make FrSCA an attractive alternative to solution-based NMR methods of conformational analysis.
Smith
Luis J
LJ
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907-2084, USA.
Boulineau
Fabien P
FP
Raftery
Daniel
D
Wei
Alexander
A
D000117:Acetylglucosamine / Q000737:chemistry; D002247:Carbon Isotopes; D005615:Freezing; D005998:Glycine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D013816:Thermodynamics
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D005998:Glycine; D000117:Acetylglucosamine