Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients.
OBJECTIVE
To investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.


METHODS
All six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.


RESULTS
All 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.


CONCLUSIONS
The unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
Wei
Shuanzeng
S
Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, China.
Liu
Tonghua
T
Liu
Hongrui
H
Gao
Jie
J
D000230:Adenocarcinoma / Q000235:genetics*; D044466:Asian People; D045744:Cell Line, Tumor; D002681:China; D003062:Codon; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*
D003062:Codon