title: Phospholipid complexation and association with apolipoprotein C-II: insights from mass spectrometry.
abstract: The interactions between phospholipid molecules in suspensions have been studied by using mass spectrometry. Electrospray mass spectra of homogeneous preparations formed from three different phospholipid molecules demonstrate that under certain conditions interactions between 90 and 100 lipid molecules can be preserved. In the presence of apolipoprotein C-II, a phospholipid binding protein, a series of lipid molecules and the protein were observed in complexes. The specificity of binding was demonstrated by proteolysis; the resulting mass spectra reveal lipid-bound peptides that encompass the proposed lipid-binding domain. The mass spectra of heterogeneous suspensions and their complexes with apolipoprotein C-II demonstrate that the protein binds simultaneously to two different phospholipids. Moreover, when apolipoprotein C-II is added to lipid suspensions formed with local concentrations of the same lipid molecule, the protein is capable of remodeling the distribution to form one that is closer to a statistical arrangement. These observations demonstrate a capacity for apolipoprotein C-II to change the topology of the phospholipid surface. More generally, these results highlight the fact that mass spectrometry can be used to probe lipid interactions in both homogeneous and heterogeneous suspensions and demonstrate reorganization of the distribution of lipids upon surface binding of apolipoprotein C-II.
authors.affiliation: University Chemistry Department, University of Cambridge, Cambridge CB2 1EW, UK.
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Hanson
authors.forename: Leopold L
authors.initials: LL
authors.lastname: Ilag
authors.forename: Jonathan
authors.initials: J
authors.lastname: Malo
authors.forename: Danny M
authors.initials: DM
authors.lastname: Hatters
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Howlett
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
mesh_terms: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000737:chemistry*; D002918:Chymotrypsin / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D007477:Ions; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D007477:Ions; D008055:Lipids; D010743:Phospholipids; D012967:Sodium Dodecyl Sulfate; D002918:Chymotrypsin