title: [Primary research of the effect of intestinal ischemia/reperfusion injury on Leptin concentrations].
abstract: OBJECTIVE
  To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses.
  
  
  METHODS
  An intestinal ischemia-reperfusion injury model of rats was established, and used a highly-sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue.
  
  
  RESULTS
  Serum Leptin level (10.82+/-0.83) microg/L significantly decreased after an injury of 60-minute ischemia and 30-minute reperfusion versus pre-experimental serum levels (16.46+/-3.21) microg/L; Leptin level in serum was higher than that in adipose tissue (4.466+/-2.63) mg/100g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended (P=0.047).
  
  
  CONCLUSIONS
  Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/reperfusion.
authors.affiliation: Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of People's Liberation Army, Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Lin
authors.forename: Guang-tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Xiu-hua
authors.initials: XH
authors.lastname: Hao
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Lu-huan
authors.initials: LH
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
mesh_terms: D000818:Animals; D005260:Female; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*
chemical_list: D020738:Leptin