Targeted degradation of TOC1 by ZTL modulates circadian function in Arabidopsis thaliana.
The underlying mechanism of circadian rhythmicity appears to be conserved among organisms, and is based on negative transcriptional feedback loops forming a cellular oscillator (or 'clock'). Circadian changes in protein stability, phosphorylation and subcellular localization also contribute to the generation and maintenance of this clock. In plants, several genes have been shown to be closely associated with the circadian system. However, the molecular mechanisms proposed to regulate the plant clock are mostly based on regulation at the transcriptional level. Here we provide genetic and molecular evidence for a role of ZEITLUPE (ZTL) in the targeted degradation of TIMING OF CAB EXPRESSION 1 (TOC1) in Arabidopsis thaliana (thale cress). The physical interaction of TOC1 with ZTL is abolished by the ztl-1 mutation, resulting in constitutive levels of TOC1 protein expression. The dark-dependent degradation of TOC1 protein requires functional ZTL, and is prevented by inhibiting the proteosome pathway. Our results show that the TOC1-ZTL interaction is important in the control of TOC1 protein stability, and is probably responsible for the regulation of circadian period by the clock.
Más
Paloma
P
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Kim
Woe-Yeon
WY
Somers
David E
DE
Kay
Steve A
SA
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001683:Biological Clocks; D002940:Circadian Rhythm / Q000502:physiology*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D009097:Multienzyme Complexes; D018749:RNA, Plant; C411822:TOC1 protein, Arabidopsis; D014157:Transcription Factors; C410503:ZTL protein, Arabidopsis; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex