Extractive spectrophotometric methods for determination of lercanidipine.
Extractive spectrophotometric methods are described for the determination of lercanidipine (LER) either in pure form or in pharmaceutical formulations. The methods involve formation of coloured chloroform extractable ion-pair complexes with bromothymol blue (BTB) and bromocresol green (BCG) in acidic medium. The extracted complexes showed absorbance maxima at 417 and 416 nm for BTB and BCG, respectively. The optimization of the reaction conditions was investigated. Beer's law is obeyed in the concentration ranges 6.0-42.0 microg x ml(-1) or 7.1-43.8 microg x ml(-1) with BTB or BCG, respectively. The composition of the ion-pairs was found to be 1:1 by Job's method. The specific absorptivities, molar absorptivities, Sandell sensitivities, standard deviations and percent recoveries were evaluated. Also, LER was determined by measurement of its first derivative signals at 245 nm. Calibration graph was established for 4.2-58.0 microg x ml(-1) of LER. The methods have been applied to the determination of drug in commercial tablets. Results of analysis were validated. No interferences were observed from common pharmaceutical adjuvants.
Erk
Nevin
N
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandoğan-Ankara, Turkey. erk@pharmacy.ankara.edu.tr
D000465:Algorithms; D001961:Bromcresol Green; D001979:Bromthymol Blue; D002121:Calcium Channel Blockers / Q000032:analysis*; D004095:Dihydropyridines / Q000032:analysis*; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D013607:Tablets; D001961:Bromcresol Green; C060343:lercanidipine; D001979:Bromthymol Blue