Inhibitory effect of hot-water extract from dried fruit of Crataegus pinnatifida on low-density lipoprotein (LDL) oxidation in cell and cell-free systems.
The dried fruit of Crataegus pinnatifida, a local soft drink material and medical herb, was found to possess potential against oxidative stress. In the preliminary study, the antioxidant potential of a hot-water extract obtained from the dried fruit of C. pinnatifida (CF-H) was evaluated in terms of its capacity of quenching 1,1-diphenyl-2-picrylhydrazyl free radicals (EC(50) = 0.118 mg/mL). After content analysis, it was found that CF-H is mainly composed of polyphenols including flavonoids (6.9%), procyanidins (2.2%), (+)-catechin (0.5%), and (-)-epicatechin (0.2%). The antioxidative bioactivity of CF-H had been assess previously using the models of CuSO(4) as cell-free system and sodium nitroprusside (SNP) plus macrophage RAW 264.7 cells as cell system to induce human low-density lipoprotein oxidation. CF-H was found to inhibit relative electrophoretic mobility and thiobarbituric acid reactive substances at the concentration of 0.5-1.0 mg/mL in the cell-free system and at 0.01-0.10 mg/mL in the cell system. Furthermore, it was found that CF-H decreased the SNP-induced cell lipid peroxidation and reduced glutathione depletion.
Chu
Chia-Yih
CY
School of Applied Chemistry, College of Health Management, and Institute of Biochemistry, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Chien Kuao N. Road, Taichung, 402 Taiwan.
Lee
Miao-Jane
MJ
Liao
Chuen-Lan
CL
Lin
Wea-Lung
WL
Yin
Yu-Fang
YF
Tseng
Tsui-Hwa
TH
D002392:Catechin / Q000494:pharmacology; D002474:Cell-Free System; D019327:Copper Sulfate / Q000737:chemistry; D027825:Crataegus / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols; D014867:Water
D005419:Flavonoids; D008077:Lipoproteins, LDL; D020030:Nitric Oxide Donors; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; D009599:Nitroprusside; D002392:Catechin; D019327:Copper Sulfate