Transcriptional regulation of the human GM3 synthase (hST3Gal V) gene during monocytic differentiation of HL-60 cells.
We studied the transcriptional regulation of human GM3 synthase (hST3Gal V) during monocytic differentiation of HL-60 cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Northern blot and reverse transcription polymerase chain reaction indicated that the induction of hST3Gal V by TPA is regulated at the transcriptional level. To elucidate the mechanism underlying the regulation of hST3Gal V gene expression during the differentiation of HL-60 cells induced by TPA, we characterized the promoter region of the hST3Gal V gene. Functional analysis of the 5'-flanking region of the hST3Gal V gene by the transient expression method showed that the -177 to -83 region, which contains a cAMP responsive element binding protein (CREB) binding site at -143, functions as the TPA-inducible promoter in HL-60 cells. In addition, gel shift assays and site-directed mutagenesis indicated that the CREB binding site at -143 is crucial for the TPA-induced expression of the hST3Gal V in HL-60 cells.
Choi
Hee-Jung
HJ
Faculty of Biotechnology, Dong-A University, Busan 604-714, South Korea.
Chung
Tae-Wook
TW
Kang
Nam-Young
NY
Kim
Kyoung-Sook
KS
Lee
Young-Choon
YC
Kim
Cheorl-Ho
CH
D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D004790:Enzyme Induction / Q000187:drug effects; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology*; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D015854:Up-Regulation
D017362:Cyclic AMP Response Element-Binding Protein; D012333:RNA, Messenger; D008156:Luciferases; D012799:Sialyltransferases; C021343:haematoside synthetase; D013755:Tetradecanoylphorbol Acetate