VARICOSE, a WD-domain protein, is required for leaf blade development.
To gain insight into the processes controlling leaf development, we characterized an Arabidopsis mutant, varicose (vcs), with leaf and shoot apical meristem defects. The vcs phenotype is temperature dependent; low temperature growth largely suppressed defects, whereas high growth temperatures resulted in severe leaf and meristem defects. VCS encodes a putative WD-domain containing protein, suggesting a function involving protein-protein interactions. Temperature shift experiments indicated that VCS is required throughout leaf development, but normal secondary vein patterning required low temperature early in leaf development. The low-temperature vcs phenotype is enhanced in axr1-3 vcs double mutants and in vcs mutants grown in the presence of polar auxin transport inhibitors, however, vcs has apparently normal auxin responses. Taken together, these observations suggest a role for VCS in leaf blade formation.
Deyholos
Michael K
MK
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Cavaness
G Frank
GF
Hall
Branden
B
King
Ed
E
Punwani
Jayson
J
Van Norman
Jaimie
J
Sieburth
Leslie E
LE
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D018519:Meristem / Q000235:genetics / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D011392:Proline; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D017931:DNA Primers; C483689:VARICOSE protein, Arabidopsis; D011392:Proline