Surface translocation of pactolus is induced by cell activation and death, but is not required for neutrophil migration and function.
Pactolus is a cell surface protein expressed by murine neutrophils. Pactolus is similar to the beta integrins, except it lacks a functional metal ion-dependent adhesion site domain and is expressed without an alpha-chain partner. The majority of the Pactolus protein is held within the cell in dense granules in a highly glycosylated form. This intracellular form of Pactolus can be released to the cell surface following inflammatory activation or ligation of Pactolus on the cell surface. In addition, intracellular Pactolus translocates to the neutrophil surface following induction of apoptosis. Neutrophil activation studies suggest that Pactolus does not serve as an activating or phagocytic receptor for the neutrophil. To further define the function of Pactolus, a Pactolus-null mouse was generated. Pactolus-deficient animals mature appropriately and possess normal numbers of neutrophils, display appropriate migration into sites of inflammation, and combat introduced infections efficiently. These data suggest that Pactolus does not function as a neutrophil phagocytic or adhesion receptor, but may instead serve as a sugar-bearing ligand for lectin recognition by other cells.
Garrison
Sean
S
Division of Cell Biology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Hojgaard
Andrias
A
Margraf
Rebecca
R
Weis
Janis J
JJ
Weis
John H
JH
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D005089:Exocytosis / Q000235:genetics / Q000276:immunology; D020022:Genetic Predisposition to Disease; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D007975:Leukotriene B4 / Q000494:pharmacology; D008565:Membrane Proteins / Q000819:agonists / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018375:Neutrophil Activation / Q000235:genetics / Q000276:immunology*; D020556:Neutrophil Infiltration / Q000235:genetics / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology; D021381:Protein Transport / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D013203:Staphylococcal Infections / Q000235:genetics / Q000276:immunology; D019311:Staurosporine / Q000494:pharmacology
D019012:Integrin beta1; D008565:Membrane Proteins; C111859:pactolus; D007975:Leukotriene B4; D019311:Staurosporine