Characterization of a novel set of resident intrathyroidal bone marrow-derived hematopoietic cells: potential for immune-endocrine interactions in thyroid homeostasis.
Immunofluorescent staining of thyroid tissues was done using monoclonal antibodies to dendritic cell (DC), lymphocyte, macrophage and granulocyte markers. Despite the presence of occasional CD11c+ cells, CD11b+ cells, morphologically characteristic of DCs, were abundant in thyroid of normal mice, at a density of approximately 2.0 cells per thyroid follicle, and were >tenfold more frequent than CD11c+ cells. Thyroid tissues were non-reactive with antibodies to F4/80, CD8alpha, CD40, CD80, Gr-1, CD3, or CD19, indicating that the CD11b+ cells were not macrophages, activated DCs, granulocytes, plasmacytoid DCs, T cells or B cells. Following systemic immune activation, DCs in secondary lymphoid tissues but not in the thyroid, upregulated CD80 expression. Using radiation chimeras made from bone marrow from enhanced green fluorescent protein (EGFP) transgenic mice, EGFP+ DC-like cells were present in the thyroid from 1-20 weeks after bone marrow transfer, but were rare in the kidney and liver, although EGFP+ cells were present in secondary lymphoid tissues. Additionally, DCs generated from EGFP+ bone marrow cells localized in the thyroid of EGFP- mice following adoptive transfer. Double staining of thyroid tissue sections with antibodies to the thyroid stimulating hormone (TSH)-beta molecule and to CD11b revealed co-expression of TSHbeta and CD11b among intrathyroidal DCs. Moreover, RT-PCR analyses indicated expression of the TSHbeta gene in thyroid tissues. These findings define a novel bone marrow-derived hematopoietic cell population that resides in the thyroid of normal mice, which may have a unique role in the microregulation of thyroid physiology and homeostasis.
Klein
John R
JR
Department of Basic Sciences, Dental Branch, University of Texas Health Science Center at Houston, Houston, TX 77030, USA. john.r.klein@uth.tmc.edu
Wang
Heuy-Ching
HC
D000818:Animals; D000911:Antibodies, Monoclonal; D018122:B7-1 Antigen / Q000276:immunology; D001854:Bone Marrow Cells; D018845:CD11 Antigens / Q000276:immunology; D017931:DNA Primers; D003713:Dendritic Cells / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D006706:Homeostasis / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000166:cytology* / Q000276:immunology; D037322:Thyrotropin, beta Subunit / Q000276:immunology
D000911:Antibodies, Monoclonal; D018122:B7-1 Antigen; D018845:CD11 Antigens; D017931:DNA Primers; D037322:Thyrotropin, beta Subunit