Production and characterisation of monoclonal antibodies to ovine interleukin-5.
Monoclonal antibodies (MAbs) were developed against recombinant ovine interleukin-5 (IL-5) produced in the baculovirus expression vector system. One MAb, D11 (isotype IgG1), neutralised the activity of both recombinant and native sources of IL-5 in a biological assay (Baf cell assay) but was only weakly reactive in immunocytochemistry. Conversely, a second MAb, A8 (isotype IgA), successfully detected IL-5 in immunocytochemistry but did not display neutralising activity. The development of these MAbs will enable the assay of ovine IL-5 in vitro and permit studies into the role of hypersensitivity reactions in sheep by neutralisation of IL-5 in vivo.
Bendixsen
Tuan
T
CSIRO Livestock Industries, Prospect, Sydney, NSW 2148, Australia.
Bosward
Katrina L
KL
Emery
David L
DL
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D005822:Genetic Vectors; D015848:Interleukin-5 / Q000097:blood* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012756:Sheep; D014162:Transfection; D014254:Trichostrongylus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015848:Interleukin-5; D011994:Recombinant Proteins