title: Development of a capillary electrophoresis method for the determination of allopurinol and its active metabolite oxypurinol.
abstract: A simple and sensitive capillary zone electrophoresis method with UV absorbance detection is described for the quantitation of allopurinol and its metabolite oxypurinol in aqueous solution. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was systematically investigated; these parameters included the nature and concentration of the separation buffer, pH and applied voltage. A buffer consisting of 15 mM 2-[N-cyclohexylamino]ethanesulfonic acid (CHES) adjusted to pH 8.8 was found to provide a very efficient and stable electrophoretic system for the analysis of these compounds. The optimized method was validated with respect to precision, linearity, limits of detection and quantification, accuracy and robustness. The applicability of the assay was demonstrated by analyzing these compounds in serum and allopurinol in commercial pharmaceutical preparations.
authors.affiliation: Department of Analytical Chemistry, University of Murcia, 30071, Murcia, Spain
authors.forename: Tomás
authors.initials: T
authors.lastname: Pérez-Ruiz
authors.forename: Carmen
authors.initials: C
authors.lastname: Martínez-Lozano
authors.forename: Virginia
authors.initials: V
authors.lastname: Tomás
authors.forename: Raquel
authors.initials: R
authors.lastname: Galera
mesh_terms: D000493:Allopurinol / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010117:Oxypurinol / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000493:Allopurinol; D010117:Oxypurinol