"In vitro" effect of cumene hydroperoxide on hepatic elongation factor-2 and its protection by melatonin.
We have examined by immunoblotting the effect of three oxidant compounds on the level of hepatic elongation factor-2 (eEF-2). Rat liver homogenates were exposed to cumene hydroperoxide (CH), 2-2'-azobis (2-aminopropane) dihydrochloride (AAPH) and H(2)O(2). Only CH treatment produced the disappearance of eEF-2, probably due to a phenomena of peptide bond cleavage. The direct implication of free radical species in this process is evident because of the fact that the inclusion of a free radical scavenger such as melatonin prevented the eEF-2 depletion. The results also suggest that the disappearance of eEF-2 induced by CH can be linked to a lipid peroxidant process, which could account for the decline of protein synthesis in aging and other circumstances where lipid peroxidation is high.
Parrado
J
J
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, C/. Tramontana s/n, 41012 Seville, Spain.
Absi
E H
EH
Machado
A
A
Ayala
A
A
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D010084:Oxidation-Reduction; D020652:Peptide Elongation Factor 2 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001555:Benzene Derivatives; D020652:Peptide Elongation Factor 2; D008550:Melatonin; C007164:cumene hydroperoxide