Using hydrophilic adhesive tape for collection of evidence for forensic DNA analysis.
Known exemplar samples of human DNA have traditionally been body fluids, such as blood, saliva, and semen. In each case, the presence of water is a risk for the bacterial growth, which may degrade the DNA evidence. In this study, the authors have developed a method that employed a hydrophilic adhesive tape (HAT) for collecting DNA evidence. The HAT method was used to remove surface cells from relatively hairless areas on the body. The area examined were ankle, arm, behind the ear, between fingers and back of the neck. The HAT was then dissolved in the extraction buffer. DNA typing was performed at vWA, THo1, F13A1, and FES loci using the short tandem repeat (STR) analysis. Our results show that the samples collected from ear give the best results with a success rate of 100%. All subjects tested by this method had known STR genotypes established from buccal swabs. The authors' results suggest that the HAT method can be used as a less invasive method for collecting biological evidence for forensic DNA analysis. In addition, this collection method should reduce the risk of DNA degradation due to the moisture, which is encountered using conventional collecting methods.
Li
Richard C
RC
Forensic Science Program, College of Criminal Justice, Sam Houston State University, Huntsville, TX 77341-2296, USA. cjc_rcl@shsu.edu
Harris
Howard A
HA
D000269:Adhesives; D002021:Buffers; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D004492:Edetic Acid; D005260:Female; D005554:Forensic Medicine / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D012867:Skin / Q000378:metabolism; D020080:Tandem Repeat Sequences; D014325:Tromethamine
D000269:Adhesives; D002021:Buffers; D014325:Tromethamine; D004247:DNA; D004492:Edetic Acid