A sequence motif within trypanosome precursor tRNAs influences abundance and mitochondrial localization.
Trypanosoma brucei lacks mitochondrial genes encoding tRNAs and must import nuclearly encoded tRNAs from the cytosol. The mechanism and specificity of this process remain unclear. We have identified a unique sequence motif, YGG(C/A)RRC, upstream of the genes encoding mitochondrially localized tRNAs in T. brucei. Both in vitro import studies and in vivo transfection studies indicate that deletion of the YGG(C/A)RRC sequence alters mitochondrial localization of tRNA(Leu), and in vivo studies also show a decrease in the cellular abundance of tRNA(Leu). These studies provide direct evidence for cis-acting RNA motifs within precursor tRNAs that facilitate the selection of tRNAs for mitochondrial import in trypanosomes. Furthermore, we found that mutations to the YGG(C/A)RRC sequence also altered the intracellular distribution of other endogenous tRNAs, suggesting a general role for this sequence in tRNA trafficking in trypanosomes.
Sherrer
R Lynn
RL
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Yermovsky-Kammerer
Audra E
AE
Hajduk
Stephen L
SL
D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D017124:Conserved Sequence; D003600:Cytosol / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000235:genetics* / Q000378:metabolism*; D012356:RNA, Transfer, Leu / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
D012322:RNA Precursors; D016053:RNA, Protozoan; D012356:RNA, Transfer, Leu; C091001:pre-tRNA(Leu)