Patterns and functions of STAT activation during Drosophila embryogenesis.
The JAK/STAT pathway mediates cytokine signaling in mammals and is involved in the function and development of the hematopoietic and immune systems. To investigate the biological functions of the JAK/STAT pathway during Drosophila development, we examined the tissue-specific localization of the tyrosine-phosphorylated, or activated form of Drosophila STAT, STAT92E. Here we show that during Drosophila embryonic development STAT92E activation is prominently detected in multiple tissues and in different developmental stages. These tissues include the tracheal pits, elongating intestinal tracks, and growing axons. We demonstrate that stat92E mutants are defective in tracheal formation, hindgut elongation, and nervous system development. Conversely, STAT92E overactivation caused premature development of the tracheal and nervous systems, and over-elongation of the hindgut. These results suggest that STAT activation is involved in proper differentiation and morphogenesis of multiple tissues during Drosophila embryogenesis.
Li
Jinghong
J
Department of Biomedical Genetics, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA.
Li
Wenjun
W
Calhoun
Healani C
HC
Xia
Fan
F
Gao
Fen-Biao
FB
Li
Willis X
WX
D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004064:Digestive System / Q000196:embryology / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005775:Gastrula / Q000166:cytology / Q000378:metabolism; D053613:Janus Kinase 1; D009154:Mutation / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D050791:STAT Transcription Factors; D015398:Signal Transduction; D014132:Trachea / Q000196:embryology / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D050791:STAT Transcription Factors; C105675:Stat92E protein, Drosophila; D015534:Trans-Activators; D011505:Protein-Tyrosine Kinases; D053613:Janus Kinase 1