Bacterial antimutagenesis by hydroxycinnamic acids from plant cell walls.
We have determined the abilities of (E)-ferulic acid, (E)-p-coumaric acid and (E,E)-5-5-dehydrodiferulic acid to protect against different types of mutation in a simple bacterial model. These antimutagenic properties were compared with those of the related compound curcumin, and also with those of an extract containing hydroxycinnamic acids obtained by the saponification of the cell walls of wheat coleoptiles. Three known mutagens, bleomycin, hydrogen peroxide and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) were used to chemically induce reversion mutation, while the known antimutagen Trolox was used as a positive control. Both the pure hydroxycinnamic acids and the extract from the cell walls showed antimutagenic properties. It is known that hydroxycinnamic acids ester-linked to plant cell walls can be released in the human colon by the action of microbial esterases. Providing the current data extrapolate to mammalian cells, they suggest that antimutagenic properties of hydroxycinnamic acids released from plant cell walls could play a role in dietary fibre protection against cancer.
Ferguson
Lynnette R
LR
Discipline of Nutrition, Faculty of Medicine and Health Sciences, The University of Auckland, Private Bag 92019, Auckland 1000, New Zealand. l.ferguson@auckland.ac.nz
Lim
Isabel Fong
IF
Pearson
Amira E
AE
Ralph
John
J
Harris
Philip J
PJ
D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001761:Bleomycin / Q000633:toxicity; D002473:Cell Wall / Q000737:chemistry; D018548:Cotyledon / Q000737:chemistry / Q000166:cytology; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects*; D011804:Quinolines / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D014908:Triticum / Q000737:chemistry* / Q000166:cytology
D016587:Antimutagenic Agents; D003373:Coumaric Acids; D009153:Mutagens; D011804:Quinolines; D001761:Bleomycin; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D006861:Hydrogen Peroxide