Effect of substance P on gene expression of transforming growth factor beta-1 and its receptors in rat's fibroblasts.
OBJECTIVE
To investigate the effect of substance P (SP) on gene expression of transforming growth factor beta-1 (TGFbeta-1), transforming growth factor receptor-1 (TGFR-1) and transforming growth factor receptor-2 (TGFR-2) in fibroblasts cultured in vitro from rat's granulation tissues.


METHODS
The fibroblasts from the granulation tissues in the skeletal muscle of rat's hind limbs injured by formaldehyde were cultured in vitro. When different concentrations (10(-9)-10(-5) mol/L) of SP were added into the culture medium, the changes of gene expression of TGFbeta-1, TGFR-1 and TGFR-2 in the cultured fibroblasts were observed with reverse transcription polymerase chain reaction at different intervals (0, 3, 6, 12 and 24 hours after incubation).


RESULTS
The gene expression of TGFbeta-1, TGFR-1 and TGFR-2 in the fibroblasts cultured from rat's granulation tissues was up-regulated by SP. The peak level of the mRNA expression was found at 10(-8) mol/L SP and the up-regulation effect was not found at 10(-5) mol/L and 10(-6) mol/L. The peak levels of gene expression of TGFbeta-1, TGFR-1 and TGFR-2 in the fibroblasts treated with SP were achieved at 6 and 12 hours, respectively.


CONCLUSIONS
SP has up-regulation effect on the gene expression of TGFbeta-1, TGFR-1 and TGFR-2 in fibroblasts from rat's granulation tissues in vitro, and the effect is related to different stimulating concentrations of SP. It may be concerned with proliferation and differentiation of fibroblasts and formation of scar tissues during wound healing.
Lai
Xi-nan
XN
Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. laixinan@163.com
Wang
Zheng-guo
ZG
Zhu
Jin-ming
JM
Wang
Li-li
LL
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011336:Probability; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018125:Receptors, Transforming Growth Factor beta / Q000187:drug effects* / Q000235:genetics*; D012680:Sensitivity and Specificity; D013373:Substance P / Q000494:pharmacology*; D014945:Wound Healing / Q000502:physiology
D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D013373:Substance P