Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals.
Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.
Holyoak
Todd
T
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA.
Fenn
Timothy D
TD
Wilson
Mark A
MA
Moulin
Aaron G
AG
Ringe
Dagmar
D
Petsko
Gregory A
GA
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003451:Cryoprotective Agents / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D008314:Malonates / Q000737:chemistry*; D008958:Models, Molecular
D001426:Bacterial Proteins; D003451:Cryoprotective Agents; D004798:Enzymes; D008314:Malonates; C030290:malonic acid