Low Mw sulfated curdlan with improved water solubility forms macromolecular complexes with polycytidylic acid.
The water solubility of curdlan was enhanced by partial sulfation at O-6. Circular dichroism measurements suggest that the sulfated curdlan with the degree of substitution (DS) from 0 to 8.7 mol% forms macromolecular complexes with polycytidylic acid (poly(C)). Although the thermal stability of the complexes decreased with increase in DS, this could be overlapped by addition of NaCl in the concentration above that of serum. The results clearly indicate that the drawback arising from the electrostatic repulsion between the anionic charges can be partially compensated by the presence of salt. Furthermore, the polynucleotide chain complexed with the sulfated curdlan was protected from the enzymatic hydrolysis, corroborating the assumption that the sulfated curdlan has an ability to bind poly(C).
Koumoto
Kazuya
K
Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
Umeda
Mariko
M
Numata
Munenori
M
Matsumoto
Takahiro
T
Sakurai
Kazuo
K
Kunitake
Toyoki
T
Shinkai
Seiji
S
D000838:Anions / Q000737:chemistry; D002942:Circular Dichroism; D005936:Glucans / Q000737:chemistry*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011066:Poly C / Q000737:chemistry*; D011119:Polynucleotides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility; D055672:Static Electricity; D013431:Sulfates / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry*; D047071:beta-Glucans
D000838:Anions; D005936:Glucans; D046911:Macromolecular Substances; D011119:Polynucleotides; D011134:Polysaccharides; D013431:Sulfates; D047071:beta-Glucans; D014867:Water; D011066:Poly C; D012965:Sodium Chloride; C063130:curdlan sulfate