Over-expression of wild-type Securin leads to aneuploidy in human cells.
Changes in gene expression and aneuploidy are both common features of cancers and under- or over-expression of single genes can cause aneuploidy. Securin, which is involved in chromatid separation, has transforming activity in vitro and is over-expressed in many tumours. We have used a GFP-based assay for chromosome loss in human cells to investigate the effects of over-expressing Securin. We find that over-expression causes a two-fold increase in chromosome loss rate and can thus be a direct cause of aneuploidy.
Christopoulou
Lina
L
Department of Biochemistry, CRC Chromosome Molecular Biology Group, University of Oxford, South Parks Road, OX1 3QU Oxford, UK.
Moore
Jonathan D
JD
Tyler-Smith
Chris
C
D000782:Aneuploidy; D015153:Blotting, Western; D019927:Cyclin E / Q000235:genetics; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins; D009363:Neoplasm Proteins / Q000235:genetics*; D064249:Securin; D014162:Transfection; D014407:Tumor Cells, Cultured
D019927:Cyclin E; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human; D049452:Green Fluorescent Proteins