title: Leptin and leptin receptor in human seminal plasma and in human spermatozoa.
abstract: Leptin, a 167 amino acid peptide, is known to influence the gonads via direct and indirect effects. Recent studies provide contradictory proposition about the peripheral impact of leptin in the male gonads. Thus, we examined leptin and its receptors in human seminal plasma and in human ejaculated spermatozoa by Western blot technique and fluorescence microscopy. In seminal plasma we found a free leptin band (16 kDa) by an anti-leptin polyclonal antibody. Incubation of seminal plasma with recombinant leptin caused a statistically significant increase in the amount of free leptin (p < 0.01) and supports this finding. Furthermore, a soluble leptin receptor (145 kDa) was found in human seminal plasma in the same specimen. We also detected a 145-kDa leptin receptor isoform in ejaculated spermatozoa as a possible target of leptin action in the male genital tract, which was localized at the tail of spermatozoa by immunofluorescence microscopy only. This receptor was significantly associated with the intactness of sperm plasma membranes. Spermatozoa with deteriorated membranes contained 49.2 +/- 6.9% leptin receptor signal intensity compared with spermatozoa having intact membranes (p < 0.01). This finding is difficult to interpret and may be caused by a leakage of OB-R due to loss of membrane integrity. In conclusion, these data provide further hints for a peripheral role of leptin in the male genital tract, possibly, by an interaction between leptin and spermatozoa via sperm leptin receptors.
authors.affiliation: Department of Dermatology, Andrological Unit, University of Leipzig, Leipzig, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Jope
authors.forename: A
authors.initials: A
authors.lastname: Lammert
authors.forename: J
authors.initials: J
authors.lastname: Kratzsch
authors.forename: U
authors.initials: U
authors.lastname: Paasch
authors.forename: H-J
authors.initials: HJ
authors.lastname: Glander
mesh_terms: D015153:Blotting, Western; D002462:Cell Membrane / Q000648:ultrastructure; D004542:Ejaculation; D006801:Humans; D007150:Immunohistochemistry; D020738:Leptin / Q000378:metabolism*; D008297:Male; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054411:Receptors, Leptin; D012661:Semen / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: C515448:LEPR protein, human; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin