Production of murine monoclonal antibody to fetal hemoglobin.
Fetal hemoglobin (Hb F) is a major hemoglobin (Hb) component at birth. Hb F levels are markedly elevated in a number of inherited and acquired disorders. Measurement of Hb F levels is usually carried out by alkali denaturation which is not very accurate for low and high values. An accurate estimation of Hb F, and also of F cells, is desired in many hematological disorders like sickle cell disease, in monitoring the efficacy of hydroxyurea (HU) therapy, to assess feto-maternal hemorrhage (FMH) during pregnancy and in the postpartum period. We have raised a murine monoclonal antibody to human Hb F, that accurately measures the number of F cells by flow cytometry. The antibody was found to be potent and specific for F cells.
Iyer
Yegneshwar S
YS
Institute of Immunohaematology, Indian Council of Medical Research (ICMR), King Edward Memorial Hospital Campus, Parel, Mumbai, India.
Patwardhan
Manisha
M
Pujari
Vaishali
V
Shinde
Gayatri
G
Keertikar
Paritosh
P
Kulkarni
Swati
S
Colah
Roshan B
RB
Mohanty
Dipika
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D005312:Fetal Blood; D005319:Fetal Hemoglobin / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry / Q000592:standards; D006801:Humans; D007118:Immunoassay / Q000592:standards; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D005319:Fetal Hemoglobin