Extracellular matrix proteins modulate cytokine production by monocytes during their interactions with cancer cells.
BACKGROUND
This study examined the role of extracellular matrix compounds (EMC) in the alteration of tumour necrosis factor-alpha (TNF alpha) and interleukin-10 (IL-10) production by human monocytes stimulated with cancer cells.


METHODS
Monocytes were cultured with cancer cells in the absence or presence of EMC and cytokine release was measured by ELISA. In some experiments monocytes preincubated with monoclonal antibodies (mAbs) against CD29 and CD44 were used.


RESULTS
Fibronectin, collagen type I and type IV induced production of cytokines by monocytes and mAbs inhibited this effect. The release of TNF alpha monocytes stimulated with cancer cells was inhibited by fibronectin, collagen type I and IV and IL-10 by fibronectin and collagen type IV. Other EMC were ineffective. Both mAbs partly reversed this inhibitory effect.


CONCLUSIONS
These findings suggest that some EMC induced cytokine release by monocytes but inhibit monocyte-cancer cell interactions and this effect is presumably due to competition for the same receptors.
Macura-Biegun
Anna
A
Department of Clinical Immunology, Polish-American Institute of Pediatrics, Jagiellonian University Medical College, Wielicka 265, 30-663 Cracow, Poland.
Ruggiero
Irena
I
Hajto
Barbara
B
Zembala
Marek
M
D000230:Adenocarcinoma / Q000473:pathology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002450:Cell Communication / Q000502:physiology*; D024042:Collagen Type I / Q000494:pharmacology; D024141:Collagen Type IV / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D019012:Integrin beta1 / Q000276:immunology; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D024042:Collagen Type I; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10