title: Thyroid hormone reduces the loss of axotomized sensory neurons in dorsal root ganglia after sciatic nerve transection in adult rat.
abstract: We have shown that a local administration of thyroid hormones (T3) at the level of transected rat sciatic nerve induced a significant increase in the number of regenerated axons. To address the question of whether local administration of T3 rescues the axotomized sensory neurons from death, in the present study we estimated the total number of surviving neurons per dorsal root ganglion (DRG) in three experimental group animals. Forty-five days following rat sciatic nerve transection, the lumbar (L4 and L5) DRG were removed from PBS-control, T3-treated as well as from unoperated rats, and serial sections (1 microm) were cut. The physical dissector method was used to estimate the total number of sensory neurons in the DRGs. Our results revealed that in PBS-control rats transection of sciatic nerve leads to a significant (P < 0.001) decrease in the mean number of sensory neurons (8743.8 +/- 748.6) compared with the number of neurons in nontransected ganglion (mean 13,293.7 +/- 1368.4). However, administration of T3 immediately after sciatic nerve transection rescues a great number of axotomized neurons so that their mean neuron number (12,045.8 +/- 929.8) is not significantly different from the mean number of neurons in the nontransected ganglion. In addition, the volume of ganglia showed a similar tendency. These results suggest that T3 rescues a high number of axotomized sensory neurons from death and allows these cells to grow new axons. We believe that the relative preservation of neurons is important in considering future therapeutic approaches of human peripheral nerve lesion and sensory neuropathy.
authors.affiliation: Institute of Cell Biology and Morphology (IBCM), Medical School, University Hospital of Lausanne, 1011-, Lausanne, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Schenker
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kraftsik
authors.forename: Liliane
authors.initials: L
authors.lastname: Glauser
authors.forename: Thierry
authors.initials: T
authors.lastname: Kuntzer
authors.forename: Julien
authors.initials: J
authors.lastname: Bogousslavsky
authors.forename: Ibtissam
authors.initials: I
authors.lastname: Barakat-Walter
mesh_terms: D000818:Animals; D019771:Axotomy; D002021:Buffers; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D010710:Phosphates; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries*; D013963:Thyroid Hormones / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D002021:Buffers; D010710:Phosphates; D013963:Thyroid Hormones; D014284:Triiodothyronine