title: Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes.
abstract: Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), Affiliated with Université Joseph Fourier, B.P. 53, 38041 Grenoble Cedex 9, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Greffe
authors.forename: Morten T
authors.initials: MT
authors.lastname: Jensen
authors.forename: Claude
authors.initials: C
authors.lastname: Bosso
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
authors.forename: Hugues
authors.initials: H
authors.lastname: Driguez
mesh_terms: D000687:Amylopectin / Q000378:metabolism; D001665:Binding Sites; D005964:Glucosyltransferases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology; D007700:Kinetics; D008320:Maltose / Q000031:analogs & derivatives / Q000378:metabolism; D025942:Oligosaccharides, Branched-Chain / Q000138:chemical synthesis* / Q000378:metabolism*; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D025942:Oligosaccharides, Branched-Chain; D011134:Polysaccharides; D008320:Maltose; D000687:Amylopectin; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D006026:Glycoside Hydrolases