title: Karyotypic characterization of representatives from Melolonthinae (Coleoptera: Scarabaeidae): karyotypic analysis, banding and fluorescent in situ hybridization (FISH).
abstract: Meiotic chromosomes of Phyllophaga (Phytalus) vestita, Phyllophaga (Phyllophaga) aff capillata and Lyogenys fuscus (Melolonthinae) were analyzed by conventional staining, C-banding, fluorochromes, silver nitrate and FISH. The three species had a diploid number of 2n=20 and a sex mechanism of the (Xyp; XYp) parachute type. P. (Phytalus) vestita,P. (Phyllophaga) aff capillata and Lyogenys fuscus showed pericentromeric constitutive heterochromatin (CH) in all autosomal bivalents and on X chromosomes. Staining with CMA3 and DAPI fluorochromes showed that the CH of P. (Phytalus) vestita is not specifically rich in AT and GC-base pairs, whereas in P. (Phyllophaga) aff capillata the sex bivalent and one autosomal pair were found to be enriched in GC base pairs with CMA3, and in Lyogenys fuscus CH was positive for DAPI. Silver nitrate staining revealed nucleolar remnants in all three species. However, FISH obtained a precise identification of nucleolar organizing regions with an rDNA 18S and 25S probe. A signal of hybridization was seen in each species, being detected in the X chromosome of P. (Phytalus) vestita and Lyogenys fuscus, and in a small autosomal bivalent of P. (Phyllophaga) aff capillata.
authors.affiliation: Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, UFPE, Brasil. mourarita@bol.com.br
authors.forename: Rita
authors.initials: R
authors.lastname: De Cássia De Moura
authors.forename: Maria José
authors.initials: MJ
authors.lastname: De Souza
authors.forename: Natoniel Franklin
authors.initials: NF
authors.lastname: De Melo
authors.forename: Amaro
authors.initials: A
authors.lastname: De Castro Lira-Neto
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D001517:Coleoptera / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006570:Heterochromatin; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male
chemical_list: D004275:DNA, Ribosomal; D006570:Heterochromatin