title: [Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs].
abstract: OBJECTIVE
  To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs.
  
  
  METHODS
  Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE.
  
  
  RESULTS
  1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves.
  
  
  CONCLUSIONS
  CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel.
authors.affiliation: Laboratory of Molecular Pharmacology, Xiangya School of Medicine, Central South University, Changsha, 410078, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.forename: Min
authors.initials: M
authors.lastname: Dong
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000502:physiology; D002957:Citrus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006168:Guinea Pigs; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018408:Patch-Clamp Techniques
chemical_list: D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal