Production and the characterization of monoclonal antibody against CD43, K06.
A monoclonal antibody against human CD43 has been developed and designated as K06. Its reactivity in the lymphoid organs was different from that of known anti-CD43 monoclonal antibodies suggesting that this may recognize a novel epitope of human CD43 molecule. The CD43 epitope detected by anti-K06 monoclonal antibody was highly expressed in cortical thymocytes, platelets, and myeloid cells of normal peripheral blood, and its reactivity was comparable to that of known anti-CD43 monoclonal antibodies. However, the density of this epitope was lower in the medullary thymocytes. Biochemical studies indicated that anti-K06 monoclonal antibody could recognize glycosylated moiety of CD43 antigen. The expression profile of anti-K06 monoclonal antibody in several cell lines was somewhat different from that of known anti-CD43 antibodies. In addition, CD43 ligation through the K06 epitope appeared to induce apoptosis in human leukemic cell line, Molt-4. We therefore assume that K06 epitope of human CD43 might have some role in T-cell development.
Park
W S
WS
Department of Pathology, Kangwon National University College of Medicine, Chuncheon, Korea.
Chae
J S
JS
Jung
K C
KC
Choi
W J
WJ
Kook
M-C
MC
Bae
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D017209:Apoptosis; D001792:Blood Platelets / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D051917:Leukosialin; D022423:Myeloid Cells / Q000276:immunology; D012795:Sialoglycoproteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000033:anatomy & histology / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015704:CD4 Antigens; D016827:CD8 Antigens; D000939:Epitopes; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins