title: Capillary electrophoretic determination of theanine, caffeine, and catechins in fresh tea leaves and oolong tea and their effects on rat neurosphere adhesion and migration.
abstract: Theanine, caffeine, and catechins in fresh tea leaves and oolong tea were determined by using capillary electrophoresis (CE). CE separated these tea polyphenols from three other tea ingredients, namely, caffeine, theophylline, and theanine, within 8 min. The young leaves (apical bud and the two youngest leaves) were found to be richer in caffeine, (-)-epigallocatechin gallate (EGCg), and (-)-epicatechin gallate (ECg) than old leaves (from 5th to 7th leaves). On the other hand, the old leaves (from 8th to 10th leaves) contained higher levels of theanine, (-)-epigallocatechin (EGC), and (-)-epicatechin (EC). Results from a comparison of fresh young tea and oolong tea compositions indicated oolong tea contained more theanine and catechins than fresh young tea. Furthermore, it was found that the levels of theanine, EGC, and EGCg in young leaves rose markedly with the withering process. Caffeine did not markedly change. However, fully or partially fermented teas (oolong tea or pauchong tea) have a common initial step in the withering process. Fresh tea leaves or oolong tea extract (0.1%, w/v) markedly inhibited neurosphere adhesion, cell migration, and neurite outgrowth in rat neurospheres. Theanine (348 micrograms/mL) and caffeine at high concentration (50 micrograms/mL) did not inhibit neurosphere adhesion or migration activities, but EGCg at 20 micrograms/mL effectively inhibited neurosphere adhesion for 24 h. These results indicated that EGCg might affect neural stem cell survival or differentiation.
authors.affiliation: Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Section 1, Jen-Ai Road, Taipei, Taiwan 100.
authors.forename: Chia-Nan
authors.initials: CN
authors.lastname: Chen
authors.forename: Chia-Min
authors.initials: CM
authors.lastname: Liang
authors.forename: Jueng-Rong
authors.initials: JR
authors.lastname: Lai
authors.forename: Yao-Jen
authors.initials: YJ
authors.lastname: Tsai
authors.forename: Jyh-Shyan
authors.initials: JS
authors.lastname: Tsay
authors.forename: Jen-Kun
authors.initials: JK
authors.lastname: Lin
mesh_terms: D000818:Animals; D002110:Caffeine / Q000032:analysis* / Q000494:pharmacology; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000032:analysis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D019075:Electrophoresis, Capillary; D005260:Female; D005971:Glutamates / Q000032:analysis* / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013662:Tea / Q000737:chemistry
chemical_list: D005971:Glutamates; D013662:Tea; D002110:Caffeine; C026166:theanine; D002392:Catechin