[Study on the in vivo effect of rhG-CSF on peripheral T lymphocyte].
OBJECTIVE
To investigate the mechanisms of in vivo rhG-CSF affecting T lymphocyte.


METHODS
Peripheral blood and apheresis graft were obtained from sibling donors before and after G-CSF administration. Proliferation of peripheral blood mononuclear cells (MNC) stimulated by PHA before and after removing monocyte by plastic-adherence was detected by MTT test. Absolute number of MNC, costimulating molecules expression on antigen presenting cells (monocyte and B lymphocyte) and the number of CD(14)(+) cells before and after plastic-adherence treatment were analyzed by flow cytometry.


RESULTS
After rhG-CSF administration, absolute peripheral blood monocyte counts increased by 4.2 +/- 1.74 times. By removing monocytes, proliferation of T cells after rhG-CSF administration partly restored, but remained 20.58% lower than that before G-CSF treatment. CD(86) co-stimulating factor expression on monocyte declined (66.96 +/- 13.87)% and mean fluorescence intensity (MIF) decreased (31.31 +/- 12.91)% after rhG-CSF administration. Expression of CD(80) on B lymphocyte decreased (45.77 +/- 26.58)%.


CONCLUSIONS
The increased number of monocytes after rhG-CSF administration suppressed T-cell proliferation, which was partly the mechanism of the T-cell hyporesponsiveness. The fact that monocyte and B lymphocyte expressed low level of B(7) co-stimulating factor suggested that antigen present cells might also mediate the alteration of T-cell proliferation.
Chen
Song-he
SH
Institute of Hematology & People's Hospital, Peking University, Beijing 100044, China.
Huang
Xiao-jun
XJ
D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D014019:Tissue Donors
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor