Immunoglobulin D enhances interleukin-6 release from the KU812 human prebasophil cell line.
Despite the role of secreted immunoglobulin D (IgD) remains still largely unknown, previous studies have suggested that secreted IgD could induce basophils degranulation in some allergic asthma patients. In the present study we have searched direct evidence of the action of IgD on KU812 cells, generally classified as an immature basophilic cell line. We analyzed by flow cytometry the capacity of IgD, purified from IgD myeloma sera, to bind KU812 cells. Biotinylated monomeric IgD (mIgD) and biotinylated oligomeric IgD (oIgD) could bind KU812 cells. Blocking experiments with others immunoglobulin isotypes showed that KU812 cells expressed an unspecific receptor for IgD. However, oIgD but not mIgD enhances the release of interleukin-6 (IL-6) from KU812 cells. On the other hand, mIgD and oIgD failed to induce histamine release from KU812 cells or from cord blood derived basophils. Since IL-6 is known to induce basophil differentiation, we proposed that IgD could be implicated in allergic disorders by stimulating IL-6 release by prebasophil cells, then IL-6 could further induce an autocrine maturation of the cells.
Sechet
B
B
Laboratoire Cytokines, FRE CNRS 2224, IBMIG, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Meseri-Delwail
A
A
Arock
M
M
Wijdenes
J
J
Lecron
J C
JC
Sarrouilhe
D
D
D001491:Basophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism*; D015471:Leukemia, Basophilic, Acute / Q000276:immunology / Q000378:metabolism*
D007072:Immunoglobulin D; D015850:Interleukin-6