title: Induction of interleukin-8 gene expression by black-pigmented Bacteroides in human pulp fibroblasts and osteoblasts.
abstract: OBJECTIVE
  To investigate the effect of black-pigmented Bacteroides on the expression of interleukin (IL)-8 gene in human pulp fibroblasts and osteoblasts.
  
  
  METHODS
  The supernatants of Porphyromonas endodontalis, P. gingivalis and Prevotella intermedia were used to evaluate IL-8 gene expression in human pulp fibroblasts and osteoblasts. The levels of mRNAs were measured by the quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis.
  
  
  RESULTS
  Investigations of the time-dependence of IL-8 mRNA expression in black-pigmented Bacteroides-treated pulp fibroblasts and osteoblasts revealed a rapid accumulation of the transcript after 2 h of exposure, and remained elevated throughout the 24-h incubation period. In addition, IL-8 mRNA gene expression was also found in human osteoblasts stimulated with black-pigmented Bacteroides. However, black-pigmented Bacteroides was found to be more effective in the induction of IL-8 mRNA gene expression in osteoblasts than in pulp fibroblasts (P < 0.05).
  
  
  CONCLUSIONS
  Black-pigmented Bacteroides are capable of amplifying the local immune response and promoting pulpal/periapical tissue inflammation by stimulating pulp fibroblasts and osteoblasts to express IL-8.
authors.affiliation: Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: L-C
authors.initials: LC
authors.lastname: Yang
authors.forename: F-M
authors.initials: FM
authors.lastname: Huang
authors.forename: C-S
authors.initials: CS
authors.lastname: Lin
authors.forename: C-M
authors.initials: CM
authors.lastname: Liu
authors.forename: C-C
authors.initials: CC
authors.lastname: Lai
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000704:Analysis of Variance; D001438:Bacteroidaceae / Q000235:genetics*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003782:Dental Pulp / Q000276:immunology* / Q000382:microbiology; D005347:Fibroblasts / Q000276:immunology* / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D010006:Osteoblasts / Q000276:immunology* / Q000382:microbiology; D045202:Porphyromonas endodontalis / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000235:genetics; D018713:Prevotella intermedia / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger