Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction.
Human leucocyte antigen-B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence-specific primer-based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence-specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases.
Zino
E
E
HLA Tissue Typing Laboratory, Immunohematology and Transfusion Medicine Service, IRCCS Istituto Scientifico H.S. Raffaele, Milan, Italy.
Di Terlizzi
S
S
Carugo
C
C
Baggi
L
L
Galli
A
A
Bonini
C
C
Rossini
S
S
Mazzi
B
B
Fleischhauer
K
K
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D015796:HLA-B27 Antigen / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015796:HLA-B27 Antigen