In vitro and in vivo antiviral properties of sulfated galactomannans against yellow fever virus (BeH111 strain) and dengue 1 virus (Hawaii strain).
Two galactomannans, one extracted from seeds of Mimosa scabrella, having a mannose to galactose ratio of 1.1, and another with a 1.4 ratio from seeds of Leucaena leucocephala, were sulfated. The products from M. scabrella (BRS) and L. leucocephala (LLS) had a degree of sulfation of 0.62 and 0.50, and an average molecular weight of 620x10(3) and 574x10(3) gmol(-1), respectively. Their activities against yellow fever virus (YFV; BeH111 strain) and dengue 1 virus (DEN-1; Hawaii strain) were evaluated. This was carried out in young mice following intraperitoneal infection with YFV. At a dose of 49 mgkg(-1), BRS and LLS gave protection against death in 87.7 and 96.5% of the mice, respectively. When challenged with 37.5 LD50 of YFV, mice previously inoculated with BRS+virus or LLS+virus, showed 93.3 and 100% resistance, respectively, with neutralization titers similar to mice injected with 25 LD50 of formaldehyde-inactivated YFV. In vitro experiments with YFV and DEN-1 in C6/36 cell culture assays in 24-well microplates showed that concentrations that produced a 100-fold decrease in virus titer of YFV were 586 and 385 mgl(-1) for BRS and LLS, respectively. For DEN-1 they were 347 and 37 mgl(-1), respectively. Sulfated galactomannans, thus demonstrate in vitro and in vivo activity against flaviviruses.
Ono
Lucy
L
Laboratório de Biopolímeros, Departamento de Química, Setor de Ciências Exatas, Centro Politécnico, Universidade Federal do Paraná, CxP 19081, Jardim das Américas, CEP 81531-990 Curitiba, PR, Brazil.
Wollinger
Wagner
W
Rocco
Iray M
IM
Coimbra
Terezinha L M
TL
Gorin
Philip A J
PA
Sierakowski
Maria-Rita
MR
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D003716:Dengue Virus / Q000187:drug effects* / Q000254:growth & development; D007887:Fabaceae; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D031298:Mimosa; D008970:Molecular Weight; D009500:Neutralization Tests; D008517:Phytotherapy; D011506:Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D013431:Sulfates / Q000032:analysis; D015004:Yellow Fever / Q000188:drug therapy* / Q000276:immunology; D015005:Yellow fever virus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
D000914:Antibodies, Viral; D000998:Antiviral Agents; D002241:Carbohydrates; D008351:Mannans; D011506:Proteins; D013431:Sulfates; C012990:galactomannan; D005690:Galactose