Luminescent enzyme-linked receptor assay for estrogenic compounds.
The analytics of endocrine-disrupting compounds has become a major issue during recent years. Several test systems have been developed for endocrine-disrupting chemicals. Yeast reporter gene assays and MCF-7 cell-based proliferation assays (E-screen) are particularly popular. A correlation of an enzyme-linked receptor assay (ELRA) with a yeast reporter gene assay is shown. In addition, the development of an ultra-sensitive luminescent ELRA with a detection limit of 20 ng/L for 17 beta-estradiol in the sample is reported. Data for real sample analysis are shown in this paper. ELRA characteristics are compared with cell-based assays, and the issue of detection limits is addressed. In this context, the detection limits of the cell-based assays have been claimed to be below the ELRA detection limits. However, it is clarified that the given detection limits for the yeast estrogen screen and the E-screen are usually based on concentrations of 17 beta-estradiol in the well, not in the sample, whereas ELRA detection limits are concentrations in the sample.
Seifert
Martin
M
Center of Life Sciences Weihenstephan, Chair of Cell Biology, Technische Universitaet Muenchen, Alte Akademie 12, 85350 Freising, Germany. seifert@wzw.tum.de
D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements; D011960:Receptors, Estrogen / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity
D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal; D011960:Receptors, Estrogen; D011994:Recombinant Proteins