Transcriptional regulation through glutamate receptors: Involvement of tyrosine kinases.
Glutamate receptors play a key role in neuronal plasticity, learning and memory, and in several neuropathologies. Short-term and long-term changes in synaptic efficacy are triggered by glutamate. Although an enhanced glutamate-dependent tyrosine phosphorylation has been described in several systems, its role in membrane-to-nuclei signaling is unclear. Taking advantage of the fact that the gene encoding the chick kainate-binding protein undergoes a glutamate-dependent transcriptional regulation via an activator protein-1 (AP-1) site, we evaluated the involvement of tyrosine kinases in this process. We describe here the participation of receptor and non-receptor tyrosine kinases in the signaling cascade triggered by glutamate. Our results suggest that in Bergmann glia cells, glutamate receptors transactivate receptor tyrosine kinases, favoring the idea of a complex network of signals activated by this excitatory neurotransmitter that results in regulation of gene expression.
López-Bayghen
Esther
E
Departamento de Genética y Biología Molecular, Cinvestav-IPN, México, D.F., México.
Aguirre
Adán
A
Ortega
Arturo
A
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D009457:Neuroglia / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D010766:Phosphorylation; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D018091:Receptors, AMPA / Q000502:physiology; D017470:Receptors, Glutamate / Q000502:physiology*; D017451:Receptors, Somatomedin / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000502:physiology*; D014162:Transfection; D019061:src-Family Kinases / Q000502:physiology*
D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D017451:Receptors, Somatomedin; D019061:src-Family Kinases