title: NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1).
abstract: A strain of Saccharomyces cerevisiae lacking the GPD2 gene, encoding one of the glycerol-3-phosphate dehydrogenases, grows slowly under anaerobic conditions, due to reductive stress caused by the accumulation of cytoplasmic NADH. We used 2D-PAGE to study the effect on global protein expression of reductive stress in the anaerobically grown gpd2Delta strain. The most striking response was a strongly elevated expression of Tdh1p, the minor isoform of glyceraldehyde-3-phosphate dehydrogenase. This increased expression could be reversed by the addition of acetoin, a NADH-specific redox sink, which furthermore largely restored anaerobic growth of the gpd2Delta strain. Additional deletion of the TDH1 gene (but not of TDH2 or TDH3) improved anaerobic growth of the gpd2Delta strain. We therefore propose that TDH1 has properties not displayed by the other TDH isogenes and that its expression is regulated by reductive stress caused by an excess of cytoplasmic NADH.
authors.affiliation: Department of Chemistry and Bioscience/Molecular Biotechnology, Chalmers University of Technology, 405-30 Göteborg, Sweden.
authors.forename: Hadi
authors.initials: H
authors.lastname: Valadi
authors.forename: Asa
authors.initials: A
authors.lastname: Valadi
authors.forename: Ricky
authors.initials: R
authors.lastname: Ansell
authors.forename: Lena
authors.initials: L
authors.lastname: Gustafsson
authors.forename: Lennart
authors.initials: L
authors.lastname: Adler
authors.forename: Joakim
authors.initials: J
authors.lastname: Norbeck
authors.forename: Anders
authors.initials: A
authors.lastname: Blomberg
mesh_terms: D000093:Acetoin / Q000494:pharmacology; D000332:Aerobiosis; D000693:Anaerobiosis; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression / Q000187:drug effects; D005800:Genes, Fungal; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D007527:Isoenzymes; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; D000093:Acetoin; D005993:Glycerolphosphate Dehydrogenase; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); C465224:TDH1 protein, S cerevisiae