title: In vivo implants of beta-sitosterol cause reductions of reactive cholesterol pools in mitochondria isolated from gonads of male goldfish (Carassius auratus).
abstract: beta-Sitosterol, a phytosterol found in high concentrations in pulp mill effluents, has been proposed as one of the causative agents for steroid depressions observed in fish exposed to pulp mill effluents. Previous studies have suggested a cholesterol-mediated mechanism; however, it is unknown how beta-sitosterol depresses gonadal steroidogenesis. In this study, adult male goldfish (Carassius auratus) were exposed for 24-31 days to beta-sitosterol (55% of a phytosterol mixture or 96% pure; 150 microg/g; Silastic implant) after which gonadal mitochondria were isolated. Pregnenolone production, an indicator of the size of the pool of reactive cholesterol, was then measured in the isolated mitochondria. Sterol exposure did not affect P450 side-chain cleavage enzyme (converts cholesterol to pregnenolone) activity but did decrease the size of the mitochondrial pool of reactive cholesterol, suggesting beta-sitosterol is impeding cholesterol transfer across the mitochondrial membrane. This finding is supported by the observation that 25-hydroxycholesterol, which passes through mitochondrial membranes without need for a membrane transporter, restores beta-sitosterol-induced reductions in pregnenolone production.
authors.affiliation: Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, E2L 4L5 New Brunswick, Canada.
authors.forename: Frédéric D L
authors.initials: FD
authors.lastname: Leusch
authors.forename: Deborah L
authors.initials: DL
authors.lastname: MacLatchy
mesh_terms: D000818:Animals; D001692:Biological Transport; D002784:Cholesterol / Q000032:analysis / Q000493:pharmacokinetics*; D006054:Goldfish / Q000502:physiology*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000502:physiology; D011284:Pregnenolone / Q000096:biosynthesis; D012855:Sitosterols / Q000008:administration & dosage / Q000494:pharmacology*; D013737:Testis / Q000502:physiology*
chemical_list: D000960:Hypolipidemic Agents; D012855:Sitosterols; C025473:gamma-sitosterol; D011284:Pregnenolone; D002784:Cholesterol