title: In vitro and short-term in vivo characteristics of a Kel-F thin film modified glucose sensor.
abstract: A new outer layer composition, consisting of polytetrafluoroethylene (PTFE), Kel-F oil, and Nafion, is suggested to minimize the detrimental effect of dissolved oxygen and to extend the linear response range of a glucose oxidase(GOx)-based sensor using nonconducting polymer. The morphology of Kel-F/PTFE/Kel-F/Nafion polymeric laminate was followed during fabrication by SEM. When Kel-F film was formed on the PTFE outer layer, the linear response was extended to 21 mM, at a sensitivity of 2.8 +/- 0.8 nA/mM mm2. We demonstrate that a sensor without Kel-F/PTFE/Kel-F/Nafion outer layer is relatively oxygen dependent, whereas by comparison a sensor with Kel-F/PTFE/Kel-F/Nafion outer layer is oxygen independent. The current of such a glucose sensor implanted in the subcutaneous tissue stabilized within 60 min, and the lag between blood glucose changes and sensor output was within 1 min. The in vivo characteristics of the glucose sensor described show great promise for one-point in vivo calibration.
authors.affiliation: Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul 110-744, Korea.
authors.forename: Sun Kil
authors.initials: SK
authors.lastname: Kang
authors.forename: Ran-A
authors.initials: RA
authors.lastname: Jeong
authors.forename: Sejin
authors.initials: S
authors.lastname: Park
authors.forename: Taek Dong
authors.initials: TD
authors.lastname: Chung
authors.forename: Sunmin
authors.initials: S
authors.lastname: Park
authors.forename: Hee Chan
authors.initials: HC
authors.lastname: Kim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D011138:Polytetrafluoroethylene; D005949:Glucose Oxidase; D010100:Oxygen