title: Crystallization and preliminary X-ray diffraction data of Mycobacterium tuberculosis FbpC1 (Rv3803c).
abstract: The heterotrimeric antigen 85 complex (Ag85) is a major component of the cell wall of Mycobacterium tuberculosis and consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2). These play key roles in the pathogenesis of tuberculosis and in maintaining cell-wall integrity. A homologue of the Ag85 subunits ( approximately 40% identity) was recently annotated in the M. tuberculosis genome as FbpC1. Unlike the Ag85-complex components, FbpC1 lacks mycolyltransferase activity and its function remains to be established. In order to aid functional characterization, FbpC1 has been crystallized. At room temperature, tetragonal crystals of FbpC1 were obtained belonging to space group P4(1)2(1)2 (unit-cell parameters a = b = 109.9, c = 61.8 A), yet when frozen the crystals underwent a phase transition to orthorhombic symmetry, space group P2(1)2(1)2(1) (a = 59.9, b = 108.9, c = 109.9 A). Diffraction data complete to 1.7 A resolution were recorded at 100 K at the synchrotron.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, England.
authors.forename: Rosalind A
authors.initials: RA
authors.lastname: Wilson
authors.forename: Sonia
authors.initials: S
authors.lastname: Rai
authors.forename: Willian N
authors.initials: WN
authors.lastname: Maughan
authors.forename: Laurent
authors.initials: L
authors.lastname: Kremer
authors.forename: Benson M
authors.initials: BM
authors.lastname: Kariuki
authors.forename: Kenneth D M
authors.initials: KD
authors.lastname: Harris
authors.forename: Trixie
authors.initials: T
authors.lastname: Wagner
authors.forename: Gurdyal S
authors.initials: GS
authors.lastname: Besra
authors.forename: Klaus
authors.initials: K
authors.lastname: Fütterer
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017356:Synchrotrons
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium; D011994:Recombinant Proteins