Thyroglobulin-pulsed human monocyte-derived dendritic cells induce CD4+ T cell activation.
Although thyroglobulin (Tg) would be expected to act as a tumor-associated antigen that might be exploitable by immunotherapy against thyroid cancers, it remains unclear how to effectively enhance the immune response to Tg in human since it is a self-component glycoprotein. We therefore tested whether and how human peripheral blood (PB) monocyte-derived dendritic cells (DCs) pulsed with human (h)Tg would induce activation of hTg-specific T cells. We found that immature DCs (iDCs) exhibited a higher endocytic capacity for fluorescein isothiocyanate-conjugated hTg than did mature DCs (mDCs). Although freshly isolated T cells responded poorly to mDCs, hTg-primed T cells responded much more strongly to hTg pulsed mDCs, which selectively induced IFN-gamma-secreting T cells. These results suggest that hTg-pulsed mDCs enhance the responses of Tg-specific T cells, raising the possibility that vaccination with hTg-pulsed mDCs may be an effective approach as immunotherapy to potentiate thyroid cancer specific therapy.
Morishita
Mariko
M
Department of Advanced Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Tokyo 108-8639, Japan. morishi-@za2.so-net.ne.jp
Uchimaru
Kaoru
K
Sato
Katsuaki
K
Ohtsuru
Akira
A
Yamashita
Shunichi
S
Kanematsu
Takashi
T
Yamashita
Naohide
N
D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D003713:Dendritic Cells / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology; D010641:Phenotype; D013954:Thyroglobulin / Q000502:physiology*
D008565:Membrane Proteins; D013954:Thyroglobulin