Androgen receptor antigen density and S-phase fraction in prostate cancer: a pilot study.
OBJECTIVE
To determine whether quantitative flow cytometric androgen receptor density expression (MFC ratio) in prostate cancer was associated with S-phase fraction.


METHODS
Flow cytometry was performed to determine DNA aneuploidy, S-phase fraction, percentage of androgen receptor (AR)-positive cells, and MFC ratio in prostate cancer patients.


RESULTS
MFC ratio showed distinct clustering. Eight patients had a low MFC ratio of 1.78-2.74, while 10 patients had high MFC ratios between 4.99 and 6.48. The S-phase fraction had average values of 11.05 vs 4.92 in tumors with high vs low MFC ratio (P<0.01).


CONCLUSIONS
S-phase fraction was significantly higher in tumors with high AR density.
Abdel-Wahab
M
M
Department of Radiation Oncology, University of Miami, Florida 33136, USA. mwahab@med.miami.edu
Krishan
A
A
Milikowski
C
C
Wahab
A A
AA
Walker
G
G
Markoe
A
A
D000368:Aged; D000728:Androgens; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011944:Receptors, Androgen / Q000378:metabolism*; D016196:S Phase; D015996:Survival Rate
D000728:Androgens; D011944:Receptors, Androgen