title: Deuterium-labeled phaseic acid and dihydrophaseic acids for internal standards.
abstract: The concentration of abscisic acid in plants is regulated not only by biosynthesis, but also by metabolism. Abscisic acid is metabolized to phaseic acid via 8'-hydroxyabscisic acid, and phaseic acid is then converted to dihydrophaseic acid and its epimer. A quantitative analysis of these metabolites is important as well as that of abscisic acid to understand changes in the concentration of abscisic acid in plants. However, no internal standards of the metabolites suitable for quantitative analysis have been reported. We prepared 7'-deuterium-labeled phaseic acid with a deuterium content of 86%, using the equilibrium reaction between phaseic acid and 8'-hydroxyabscisic acid. 7'-Deuterium-labeled dihydrophaseic acids were obtained by reducing 7'-deuterium-labeled phaseic acid. The levels of the metabolites in plant organs were determined by using the deuterated metabolites as internal standards.
authors.affiliation: International Innovation Center, Kyoto University, Japan. hirai@kais.kyoto-u.ac.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hirai
authors.forename: Satoru
authors.initials: S
authors.lastname: Kondo
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohigashi
mesh_terms: D000040:Abscisic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis; D003903:Deuterium; D035264:Flowers / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D027845:Malus / Q000737:chemistry; D008958:Models, Molecular; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D012016:Reference Values; D012717:Sesquiterpenes / Q000032:analysis* / Q000138:chemical synthesis
chemical_list: D012717:Sesquiterpenes; C000612858:phaseic acid; D000040:Abscisic Acid; D003903:Deuterium