Glycoprotein isolated from Ulmus davidiana Nakai inhibits TPA-induced apoptosis through nuclear factor-kappa B in NIH/3T3 cells.
Glycoprotein of Ulmus davidiana Nakai (UDN glycoprotein) was isolated and identified using SDS-PAGE. UDN glycoprotein was shown to have strong scavenging activities against oxygen free radicals, as detected by different oxygen-radical formation assays. To investigate the anti-apoptotic effects of UDN glycoprotein, we investigated the activity of protein kinase C alpha (PKCalpha), the DNA-binding activation of nuclear factor-kappa B (NF-kappaB), the production of nitric oxide (NO) and apoptosis in 12-O-tetradecanoylphorbol 13-acetate (TPA)-stimulated NIH/3T3 cells using a western blot analysis, electrophoretic mobility shift assays (EMSA) and NO assays. Results in this experiment showed that 100 microg/ml of UDN glycoprotein has inhibitory effects on PKCalpha translocation, NF-kappaB DNA binding activity, NO production, and apoptosis in TPA (61.68 ng/ml)-stimulated NIH/3T3 cells. Interestingly however, it could not regulate the DNA binding activity of AP-1. Therefore, UDN glycoprotein, a natural anti-oxidant, is a potential modulator of apoptotic signal pathways in NIH/3T3 cells.
Lee
Sei-Jung
SJ
Institute of Biotechnology, Chonnam National University, No. 521, 300, Yongbong-Dong 500-757, Kwangju, South Korea.
Heo
Kyung-Sun
KS
Oh
Phil-Sun
PS
Lim
Kwang
K
Lim
Kye-Taek
KT
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000502:physiology; D041681:NIH 3T3 Cells; D009569:Nitric Oxide / Q000096:biosynthesis; D010940:Plant Proteins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D034403:Ulmus / Q000737:chemistry*
D000975:Antioxidants; D006023:Glycoproteins; D016328:NF-kappa B; D010940:Plant Proteins; D009569:Nitric Oxide; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate