title: Herbimycin A induces sympathetic neuron survival and protects against hypoxia.
abstract: We have examined how herbimycin affects the survival and neuritogenesis of avian sympathetic neurons. Herbimycin promoted sympathetic neuron survival and neuritogenesis. At higher concentrations (> or = 100 ng/ml), herbimycin still enhanced neuron survival but blocked neuritogenesis. Addition of herbimycin (10-30 ng/ml) to neurons cultured in the presence of NGF or retinal conditioned medium altered neuronal morphology, with an increase in the number of neurites. Addition of NGF during hypoxia rescued 52% of the neurons compared to 14% survival in control conditions. Herbimycin alone rescued about 50% of the neurons. In the presence of NGF and 100 ng/ml herbimycin, 81% of the neurons survived hypoxia. Our results show that herbimycin promotes survival of chick sympathetic neurons and potentiates the effects of NGF.
authors.affiliation: Programa de Neurobiologia, IBCCF, UFRJ, Rio de Janeiro, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Cabral-da-Silva
authors.forename: N E Loureiro
authors.initials: NE
authors.lastname: dos Santos
authors.forename: F G
authors.initials: FG
authors.lastname: de Mello
authors.forename: J S H
authors.initials: JS
authors.lastname: Taylor
authors.forename: A M
authors.initials: AM
authors.lastname: Tolkovsky
authors.forename: R
authors.initials: R
authors.lastname: Linden
authors.forename: R A M
authors.initials: RA
authors.lastname: Reis
mesh_terms: D000320:Adrenergic Fibers / Q000187:drug effects* / Q000502:physiology*; D000818:Animals; D016227:Benzoquinones; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D047029:Lactams, Macrocyclic; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D011809:Quinones / Q000494:pharmacology*; D017828:Rifabutin / Q000031:analogs & derivatives
chemical_list: D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D011809:Quinones; D017828:Rifabutin; C020754:herbimycin