title: Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction.
abstract: Human leucocyte antigen-B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence-specific primer-based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence-specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases.
authors.affiliation: HLA Tissue Typing Laboratory, Immunohematology and Transfusion Medicine Service, IRCCS Istituto Scientifico H.S. Raffaele, Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Zino
authors.forename: S
authors.initials: S
authors.lastname: Di Terlizzi
authors.forename: C
authors.initials: C
authors.lastname: Carugo
authors.forename: L
authors.initials: L
authors.lastname: Baggi
authors.forename: A
authors.initials: A
authors.lastname: Galli
authors.forename: C
authors.initials: C
authors.lastname: Bonini
authors.forename: S
authors.initials: S
authors.lastname: Rossini
authors.forename: B
authors.initials: B
authors.lastname: Mazzi
authors.forename: K
authors.initials: K
authors.lastname: Fleischhauer
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D015796:HLA-B27 Antigen / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D015796:HLA-B27 Antigen