Evaluation of S. aureus ID, a new chromogenic agar medium for detection of Staphylococcus aureus.
S. aureus ID (bioMérieux, La Balme Les Grottes, France) is a new chromogenic agar medium designed to enable the isolation of staphylococci and the specific identification of Staphylococcus aureus. S. aureus produces green colonies on this medium due to production of alpha-glucosidase. To evaluate this medium, a total of 350 wound swabs were cultured onto S. aureus ID, CHROMagar Staph. aureus, and conventional media routinely used in our laboratory. After 18 to 20 h of incubation, 96.8% of strains formed green colonies on S. aureus ID compared with 91.1% of strains forming mauve colonies on CHROMagar Staph. aureus. A total of 94.3% of strains were recovered within 18 to 20 h with conventional media. The sensitivity was increased after 48 h of incubation to 98.7, 96.2, and 95.6% with S. aureus ID, CHROMagar Staph. aureus, and conventional media, respectively. A total of 97.4% of green colonies on S. aureus ID were confirmed as S. aureus compared with 94.4% of mauve colonies on CHROMagar Staph. aureus. We conclude that S. aureus ID is a highly sensitive and specific medium for the isolation and identification of S. aureus from wound swabs.
Perry
John D
JD
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom. john.perry@nuth.northy.nhs.uk
Rennison
Claire
C
Butterworth
Lynne A
LA
Hopley
Andrew L J
AL
Gould
F Kate
FK
D000038:Abscess / Q000382:microbiology; D000362:Agar; D003470:Culture Media; D005189:False Positive Reactions; D006801:Humans; D013203:Staphylococcal Infections / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*
D003470:Culture Media; D000362:Agar