Indole-3-butyric acid (IBA) production in culture medium by wild strain Azospirillum brasilense.
Some microorganisms found in the soil are able to produce substances which regulate plant growth. In this study, we show the presence of a substance associated with auxin activity, identified as indole-3-butyric acid (IBA), in Azospirillum brasilense UAP 154 growth medium. A. brasilense was grown and indolic compounds were extracted from the supernatant. These were then analyzed by high performance liquid chromatography (HPLC), gas chromatography and gas chromatography mass spectrometry. The retention time was similar to those of the authentic IBA standard. The compound obtained from HPLC was collected and applied to maize seedlings (Zea mays), inducing biological activity along the roots, similar to that induced by an authentic IBA standard.
Martínez-Morales
Luis Javier
LJ
Centro de Investigaciones Microbiológicas, Instituto de Ciencias, Universidad Autónoma de Puebla, Apdo Postal 1622 Puebla Pue, Mexico. jlmartin@siu.buap.mx
Soto-Urzúa
Lucía
L
Baca
Beatriz Eugenia
BE
Sánchez-Ahédo
José Antonio
JA
D015806:Azospirillum brasilense / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D007210:Indoleacetic Acids / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D003313:Zea mays / Q000187:drug effects / Q000254:growth & development
D003470:Culture Media; D007210:Indoleacetic Acids; D007211:Indoles; C014612:indolebutyric acid