[Effects of lithium chloride on the proliferation and apoptosis of K562 leukemia cells].
OBJECTIVE
To investigate the effects of lithium chloride on the proliferation and apoptosis of human leukemia cell line (K562).


METHODS
The effects of lithium chloride on the proliferation of K562 leukemia cells were evaluated by liquid culture assay, MTT assay, and colony formation assay. The effects of lithium chloride on the apoptosis of K562 leukemia cell were verified by cellular morphology and the agarose gel electrophoresis of DNA fragments.


RESULTS
1. The proliferation of K562 leukemia cells was inhibited by lithium chloride (10-50 mmol/L) in a dose-dependent manner. 2. With lithium chloride (30 mmol/L), the apoptosis cells could be observed in cellular morphology, and DNA ladder was also observed in agarose gel electrophoresis.


CONCLUSIONS
Lithium chloride can inhibit the proliferation of K562 cells and cause the apoptosis of K562 cells.
Tang
Hua-rong
HR
Department of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
He
Qun
Q
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D006801:Humans; D020014:K562 Cells; D018021:Lithium Chloride / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D018021:Lithium Chloride