title: Isolation and properties of major components of Penicillium canescens extracellular enzyme complex.
abstract: The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia. oasinitsyna@enzyme.chem.msu.ru
authors.forename: O A
authors.initials: OA
authors.lastname: Sinitsyna
authors.forename: F E
authors.initials: FE
authors.lastname: Bukhtoyarov
authors.forename: A V
authors.initials: AV
authors.lastname: Gusakov
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: A O
authors.initials: AO
authors.lastname: Bekkarevitch
authors.forename: Yu P
authors.initials: YP
authors.lastname: Vinetsky
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D010407:Penicillium / Q000201:enzymology*; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005656:Fungal Proteins; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; C019713:alpha-N-arabinofuranosidase; D044644:Endo-1,3(4)-beta-Glucanase