An invertase inhibitor from maize localizes to the embryo surrounding region during early kernel development.
Invertase activity is thought to play a regulatory role during early kernel development by converting sucrose originating from source leaves into hexoses to support cell division in the endosperm and embryo. Invertases are regulated at the posttranslational level by small protein inhibitors, INVINHs. We found that in maize (Zea mays), an invertase inhibitor homolog (ZM-INVINH1) is expressed early in kernel development, between 4 and 7 d after pollination. Invertase activity is reduced in vitro in the presence of recombinant ZM-INVINH1, and inhibition is attenuated by pre-incubation with sucrose. The presence of a putative signal peptide, fractionation experiments, and ZM-INVINH1::green fluorescent protein fusion experiments indicate that the protein is exported to the apoplast. Moreover, association of ZM-INVINH1 with the glycoprotein fraction by concanavalin A chromatogaphy suggests that ZM-INVINH1 interacts with an apoplastic invertase during early kernel development. ZM-INVINH1 was localized to the embryo surrounding region by in situ analysis, suggesting that this region forms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endosperm developmental rates.
Bate
Nicholas J
NJ
Agronomic Traits, Trait and Technology Development, Pioneer Hi-Bred International, 7250 N.W. 62nd Avenue, Johnston, Iowa, 50131-0552, USA. nic.bate@pioneer.com
Niu
Xiping
X
Wang
Yuwen
Y
Reimann
Kellie S
KS
Helentjaris
Timothy G
TG
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D004791:Enzyme Inhibitors / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000196:embryology* / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000037:antagonists & inhibitors*
D018744:DNA, Plant; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D011994:Recombinant Proteins; D043324:beta-Fructofuranosidase