Intravitreal triamcinolone does not alter basal vascular endothelial growth factor mRNA expression in rat retina.
Intravitreal triamcinolone inhibits choroidal neovascularization. To investigate if vascular endothelial growth factor (VEGF) is affected, we examined VEGF expression after intravitreal triamcinolone administration in rat retina. Using in situ hybridization, we have found dense clustered VEGF mRNA signals in the retinal pigment epithelium, moderate patchy signals in the inner nuclear layer, and positive labeling in a sub-population of ganglion cells. Densitometry and northern blot analysis revealed no significant alteration of VEGF mRNA expression pattern and level 3-21 days after triamcinolone injection. Our data indicate that intravitreal triamcinolone does not affect basal VEGF mRNA expression in normal adult rat retina.
Gao
Hua
H
Department of Ophthalmology, Baylor College of Medicine, Cullen Eye Institute, 6560 Fannin, Suite 2200, Houston, TX 77030, USA. huagao55@hotmail.com
Qiao
Xiaoxi
X
Gao
Rui
R
Mieler
William F
WF
McPherson
Alice R
AR
Holz
Eric R
ER
D020533:Angiogenesis Inhibitors / Q000627:therapeutic use*; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D002829:Choroid / Q000098:blood supply; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization / Q000379:methods; D007267:Injections; D008268:Macular Degeneration / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014221:Triamcinolone / Q000627:therapeutic use*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D014822:Vitreous Body
D020533:Angiogenesis Inhibitors; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D014221:Triamcinolone