Bundle sheath chloroplasts of rice are more sensitive to drought stress than mesophyll chloroplasts.
We investigated the effects of drought stress on the ultrastructure of chloroplasts in rice plants. After the seedlings were grown in a glasshouse for 1 month, they were treated for drought stress using two methods. One drought treatment was imposed by reducing the water supply to the plants for 1 month. The other was imposed by withholding water for 2 weeks to examine the withering process of leaves by drought stress. The ultrastructural changes of chloroplasts in bundle sheath cells were more prominent than those in mesophyll cells under both drought stress treatments. Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) content in bundle sheath chloroplasts reduced more dramatically than in mesophyll chloroplasts by drought stress. Although a slight swelling of thylakoids was sometimes observed in bundle sheath chloroplasts in moderate stress for 1 month, the thylakoids were less affected by drought stress than chloroplast envelope. These results suggest that chloroplasts in bundle sheath cells were more sensitive to drought stress than those in mesophyll cells and the thylakoids were less damaged by drought stress compared with chloroplast envelope.
Yamane
Koji
K
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Hayakawa
Koji
K
Kawasaki
Michio
M
Taniguchi
Mitsutaka
M
Miyake
Hiroshi
H
D002736:Chloroplasts / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004190:Disasters; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013997:Time Factors; D014867:Water / Q000494:pharmacology*
D014867:Water; D012273:Ribulose-Bisphosphate Carboxylase