Comparative study on serum iron determination by different methods.
Iron is an important trace element in humans. Depletion or overload iron status can result in a number of aberrant physiological functions. Determination of serum iron is one of the valuable test panels for iron status assessment. A comparative study was performed on 3 different methods of serum iron determination, nonprecipitating ferrozine colorimetric method, precipitating ferrozine colorimetric method, and iron liquicolor cleaning factor colorimetric agar-based (CAB) method (Human, Taunusstein, Germany). The correlation coefficients between nonprecipitating ferrozine colorimetric method and precipitating ferrozine colorimetric method, between nonprecipitating ferrozine colorimetric method and iron liquicolor cleaning factor CAB method, and between precipitating ferrozine colorimetric method and iron liquicolor cleaning factor CAB method were found to be 0.998 (P = .067), 0.999 (P = .067), and 0.951 (P = .934), respectively. For a setting with limited resources such as Thailand, use of the first analytical technique, nonprecipitating ferrozine colorimetric method, is recommended because of its low cost and ease of performance.
Suwansaksri
Jamsai
J
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Sookarun
Suphun
S
Wiwanitkit
Viroj
V
Boonchalermvichian
Chaiyaporn
C
Nuchprayoon
Issarang
I
D003124:Colorimetry / Q000191:economics / Q000379:methods / Q000592:standards; D004920:Erythropoiesis; D005297:Ferrozine; D006403:Hematologic Tests / Q000379:methods* / Q000592:standards; D006801:Humans; D007501:Iron / Q000097:blood*; D008722:Methods; D013789:Thalassemia / Q000097:blood
D005297:Ferrozine; D007501:Iron