Efficient repair of DNA damage induced by heavy ion particles in meiotic prophase I nuclei of Caenorhabditis elegans.
The effects of heavy ion particle irradiation on meiosis and reproductive development in the nematode Caenorhabditis elegans were studied. Meiotic pachytene nuclei are significantly resistant to particle irradiation by the heavy ions carbon and argon, as well as to X-rays, but not UV, whereas diplotene to diakinesis stage oocytes and early embryonic cells are not. Chromosomal abnormalities appear in mitotic cells and in maturing oocytes irradiated with heavy ion particles during the diplotene to the early diakinesis stages, but not in oocytes irradiated during the pachytene stage. The pachytene nuclei of ced-3 mutants, which are defective in apoptosis, are similarly resistant to ionizing radiation, but pachytene nuclei depleted for Ce-atl-1 (ataxia-telangiectasia like 1) or Ce-rdh-1/rad-51 are more sensitive. Pachytene nuclei thus appear to effectively repair heavy ion-induced DNA damage by the meiotic homologous recombination system.
Takanami
Takako
T
Graduate School of Life Sciences, Tohoku University, Saitama, Japan.
Zhang
Yongzhao
Y
Aoki
Hidetoshi
H
Abe
Tomoko
T
Yoshida
Shigeo
S
Takahashi
Hideyuki
H
Horiuchi
Saburo
S
Higashitani
Atsushi
A
D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D020450:Heavy Ions; D008540:Meiosis / Q000235:genetics / Q000528:radiation effects; D009866:Oogenesis / Q000528:radiation effects*; D010770:Phosphotransferases / Q000172:deficiency; D011418:Prophase / Q000235:genetics* / Q000528:radiation effects*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000235:genetics; D011995:Recombination, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays; D014965:X-Rays
D029742:Caenorhabditis elegans Proteins; D004247:DNA; C576885:Atl-1 protein, C elegans; D010770:Phosphotransferases; D064007:Ataxia Telangiectasia Mutated Proteins