[The experimental study of cryopreserved osteoblasts combined with BGC repairing mandibular defects in rabbits].
OBJECTIVE
The purpose of this study was to observe the biological characteristics of cultured Periosteal-derived Osteoblasts (POBs) preserved in liquid nitrogen in vitro, and to preliminarily study the osteogenetic capability of bioactive glass ceramic (BGC) combined with POBs.


METHODS
The cryopreserved cells were cultured in DMEM medium and examined by morphological and histological assay. The POBs growing well in vitro were seeded into the porous BGC materials. A week later, the combined materials were implanted into the bone defects of rabbits' mandibular, the control groups were implanted into the single BGC (no cells). After 2, 4, 8, 12 weeks of operation, the specimens were respectively excised and examined by X-ray and histological chemistry.


RESULTS
The cryopreserved POBs grew well in vitro and also had the tipical characteristics of mature osteoblasts. Cultured with BGC materials, the cells could attach, grow and proliferate well on the surface of most endoporous. After 4 weeks of operation, the transplanted osteoblasts began to form new osteoid or bone tissue in most pores of implanted BGC, and the bone defects were repaired better and earlier.


CONCLUSIONS
It was practical to use the cryopreserved osteoblasts for further study on bone tissue engineering. It suggested that the "life active" bone would get more application and play a more important role in bone restoration and reconstruction.
Wei
Feng-cai
FC
QiLu Hospital, Shandong University, Jinan 250012, China.
Tan
Wan-ye
WY
Sun
Shan-zhen
SZ
Li
Guo-ju
GJ
Hou
Huai-shui
HS
Ma
Bo-long
BL
D000818:Animals; D002516:Ceramics; D015925:Cryopreservation; D005898:Glass; D008334:Mandible / Q000601:surgery*; D010006:Osteoblasts / Q000502:physiology / Q000637:transplantation*; D011817:Rabbits; D023822:Tissue Engineering