Determination of lincomycin and tylosin residues in honey using solid-phase extraction and liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
An analytical method for the determination of residues of the antibiotic drugs lincomycin and tylosin in honey was developed. The procedure employed a solid-phase extraction for the isolation of lincomycin and tylosin from diluted honey samples. The antibiotic residues were subsequently analyzed by reversed-phase HPLC with atmospheric pressure chemical ionization mass spectrometric detection. Average analyte recoveries for lincomycin and tylosin ranged from 84 to 107% in replicate sets of honey samples fortified with drug concentrations of 0.01, 0.5, and 10 microg/g. The method detection limits were determined to be 0.007 and 0.01 microg/g for lincomycin and tylosin, respectively.
Thompson
Thomas S
TS
Alberta Agriculture, Food and Rural Development, Food Safety Division, Agri-Food Laboratories Branch, 6909 116th Street, Edmonton, Alta., Canada T6H 4P2. tom.thompson@gov.ab.ca
Noot
Donald K
DK
Calvert
Jane
J
Pernal
Stephen F
SF
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006722:Honey / Q000032:analysis*; D008034:Lincomycin / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015645:Tylosin / Q000032:analysis*
D008034:Lincomycin; D015645:Tylosin