title: Evidence for control of nitrogen metabolism by a START-dependent mechanism in Saccharomyces cerevisiae.
abstract: It is generally thought that cell growth and metabolism regulate cell division and not vice versa. Here, we examined Saccharomyces cerevisiae cells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Bryan
authors.forename: E
authors.initials: E
authors.lastname: McGrew
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Polymenis
mesh_terms: D001483:Base Sequence; D017489:CDC28 Protein Kinase, S cerevisiae / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D016193:G1 Phase / Q000502:physiology; D005800:Genes, Fungal; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C082719:CLN1 protein, S cerevisiae; C082721:CLN2 protein, S cerevisiae; D016213:Cyclins; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D005970:Glutamate Synthase; D017489:CDC28 Protein Kinase, S cerevisiae; D009584:Nitrogen