[Studies on establishing rRNA gene map of Angelica sinensis and Rheum palmatum from Gansu by DNA sequencing].
OBJECTIVE
To amplify and sequence the internal transcripted spacer of rRNA gene of the seeds of Angelica sinensis and Rheum palmatum for establishing a new method to identify the Chinese herb seeds in molecular level.


METHODS
rDNA in the seeds was extracted. Specific synthesized primers were applied to amplify the internal transcripted spacer of rRNA gene by nested PCR. And the PCR products were sequenced.


RESULTS
PCR results were confirmed by agarose electrophoresis, the above-mentioned methods could achieve the PCR products of interval region of rRNA gene in the seeds, and the sequences of the internal transcripted spacer of rRNA gene in the seeds were acquired by sequencing. There existed marked difference.


CONCLUSIONS
The base sequences of the internal transcripted spacer of rRNA gene in different plant Chinese seeds can be applied to be identifying marker in molecular level.
Zhang
Xiling
X
Gansu College of Traditional Chinese Medicine, Lanzhou 730000.
Ji
Keping
K
Li
Yingdong
Y
Chen
Che
C
Li
Xiaohong
X
Liu
Lisha
L
D029971:Angelica sinensis / Q000235:genetics*; D001483:Base Sequence; D020459:Genes, rRNA; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012250:Rheum / Q000235:genetics*; D012639:Seeds / Q000235:genetics
D005819:Genetic Markers; D018749:RNA, Plant; D012335:RNA, Ribosomal