title: Molecular detection and identification of agents of eumycetoma: detailed report of two cases.
abstract: We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.
authors.affiliation: Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
authors.forename: Abdalla O A
authors.initials: AO
authors.lastname: Ahmed
authors.forename: Nicole
authors.initials: N
authors.lastname: Desplaces
authors.forename: Philippe
authors.initials: P
authors.lastname: Leonard
authors.forename: Fred
authors.initials: F
authors.lastname: Goldstein
authors.forename: Sybren
authors.initials: S
authors.lastname: De Hoog
authors.forename: Henri
authors.initials: H
authors.lastname: Verbrugh
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000627:therapeutic use; D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D017964:Itraconazole / Q000627:therapeutic use; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009181:Mycoses / Q000175:diagnosis* / Q000473:pathology / Q000628:therapy; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D017964:Itraconazole