Characterization of plasma membrane respiratory chain and ATPase in the actinomycete Nonomuraea sp. ATCC 39727.
We have characterized the respiratory system of the aerobic actinomycete Nonomuraea sp. ATCC 39727. The plasma membrane of the microorganism is shown to contain a protonmotive respiratory chain and H+-ATPase. The respiratory chain is made up of a rotenone-sensitive NADH-quinone oxidoreductase, a four subunits aa3-type cytochrome c oxidase and a bc1 complex. The H+-ATPase is characterized as an F0F1-type on the basis of its sensitivity to specific inhibitors; the enzyme is also inhibited by mM concentrations of Ca2+. The activity of the respiratory chain increases during the exponential growth phase, but is depressed in the stationary phase. The H+-ATPase activity reaches, as the respiratory chain, a maximal activity at the end of the exponential growth phase and then remains constant in the stationary phase.
Palese
L L
LL
Department of Medical Biochemistry and Biology, University of Bari, Bari, Italy.
Gaballo
A
A
Technikova-Dobrova
Z
Z
Labonia
N
N
Abbrescia
A
A
Scacco
S
S
Micelli
L
L
Papa
S
S
D000192:Actinomycetales / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D025243:Bacterial Proton-Translocating ATPases / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D012402:Rotenone / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003486:Cyanides; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D014475:Uncoupling Agents; D012402:Rotenone; D000968:Antimycin A; D003576:Electron Transport Complex IV; D025243:Bacterial Proton-Translocating ATPases; D042967:Electron Transport Complex I; D002118:Calcium