Angular distribution of light scattered by single biological cells and oriented particle agglomerates.
We used a flow cytometer together with an intensified CCD camera to record spatially resolved light scattering from micrometer-sized single particles and single oriented particle agglomerates. Experimental differential cross sections of an oriented dumbbell made from two identical polystyrene spheres were compared with theoretical values calculated within the discrete dipole approximation, and good agreement was achieved. Furthermore, characteristic two-dimensional patterns of the scattered-light intensity were recorded for single blood cells, yielding information on the cells' shape and volume. Besides flow cytometry, we observed and analyzed differential light scatter of particle clusters of known size, shape, and orientation located within an optical trap.
Neukammer
Jörg
J
Division of Medical Physics and Metrological Information Technology, Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, 10587 Berlin, Germany. joerg.neukammer@ptb.de
Gohlke
Carsten
C
Höpe
Andreas
A
Wessel
Thomas
T
Rinneberg
Herbert
H
D000465:Algorithms; D048429:Cell Size; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007962:Leukocytes / Q000166:cytology*; D046911:Macromolecular Substances; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D008968:Molecular Conformation; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D012399:Rotation; D012542:Scattering, Radiation; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods
D046911:Macromolecular Substances; D011137:Polystyrenes