Carbon 13 NMR spectroscopy: a powerful tool for studying renal metabolism.
Using precise examples, this paper shows that carbon 13 NMR spectroscopy in conjunction with radioactive and enzymatic methods as well as with adequate mathematical modeling of metabolic pathways allows not only to identify but also to quantify fluxes through enzymes involved in substrate and drug metabolism. Carbon 13 NMR spectroscopy is a tool of unprecedented power to unravel the complexity of renal metabolism. Currently it plays a major role in what is nowadays called metabolomics.
Baverel
G
G
Inserm U499 et ANIMET, Faculté de Médecine RTH Laennec, rue Guillaume Paradin, 69372 cedex 8 Lyon, France. baverel@laennnec.univ-lyon1.fr
Conjard
A
A
Chauvin
M-F
MF
Vercoutere
B
B
Vittorelli
A
A
Dubourg
L
L
Gauthier
C
C
Michoudet
C
C
Durozard
D
D
Martin
G
G
D000818:Animals; D002247:Carbon Isotopes; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011766:Pyruvate Carboxylase / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D011817:Rabbits
D002247:Carbon Isotopes; D007773:Lactates; D011768:Pyruvate Dehydrogenase Complex; D018698:Glutamic Acid; D011766:Pyruvate Carboxylase; D005947:Glucose