title: Construction of vectors for inducible gene expression in Lactobacillus sakei and L plantarum.
abstract: We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum.
authors.affiliation: Matforsk, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. elisabeth.sorvig@matforsk.no
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Sørvig
authors.forename: Sonja
authors.initials: S
authors.lastname: Grönqvist
authors.forename: Kristine
authors.initials: K
authors.lastname: Naterstad
authors.forename: Geir
authors.initials: G
authors.lastname: Mathiesen
authors.forename: Vincent G H
authors.initials: VG
authors.lastname: Eijsink
authors.forename: Lars
authors.initials: L
authors.lastname: Axelsson
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D010957:Plasmids
chemical_list: D005966:Glucuronidase