A ribonuclease with distinctive features from the wild green-headed mushroom Russulus virescens.
A ribonuclease with an N-terminal sequence different from those of other ribonucleases has been purified from fruiting bodies of the mushroom Russula virescens. The RNase was adsorbed on DEAE-cellulose and Q-Sepharose in 10mM Tris-HCl buffer (pH 7.1-7.3) and on CM-Sepharose in 10mM NH(4)OAc buffer (pH 4.6), unlike other mushroom ribonucleases which are unadsorbed on DEAE-cellulose. The RNase demonstrated a molecular mass of 28kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In contrast to other mushroom ribonucleases which are monospecific, it exhibited co-specificity towards poly A and poly C. It demonstrated a pH optimum of 4.5, which is lower than values reported for other mushroom ribonucleases, and a temperature optimum of 60 degrees C.
Wang
Hexiang
H
Department of Microbiology, College of Biological Science, China Agricultural University, and State Key Laboratory of Agrobiotechnology, Beijing, China.
Ng
Tzi Bun
TB
D000363:Agaricales / Q000737:chemistry* / Q000145:classification / Q000201:enzymology*; D000595:Amino Acid Sequence; D002850:Chromatography, Gel / Q000379:methods; D004586:Electrophoresis; D004789:Enzyme Activation; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011061:Poly A / Q000737:chemistry*; D011066:Poly C / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D017385:Sequence Homology; D013045:Species Specificity; D013379:Substrate Specificity
D005656:Fungal Proteins; D011061:Poly A; D011066:Poly C; D012260:Ribonucleases