Measurement of carvedilol in plasma by high-performance liquid chromatography with electrochemical detection.
Carvedilol is a beta/alpha1-adrenoceptor blocker. A sensitive method for measuring plasma levels of carvedilol in human administrated low doses is needed since its plasma concentration is low. We measured carvedilol and carvedilol M21-aglycon using high-performance liquid chromatography (HPLC) with electrochemical detection. The amperometric detector was operated at 930 mV versus Ag/AgCl. Mean coefficients of variation (n = 5) for carvedilol and M21-aglycon were 4.0 and 7.7% (intra) and 6.1 and 6.7% (inter), respectively. The lower limit of quantification for each analyte was 0.10 ng/ml (signal-to-noise ratio = 3). This lower limit of quantification for carvedilol was sufficient for clinical use.
Machida
Maiko
M
Department of Pathophysiology, Hokkaido College of Pharmacy, 7-1 Katsuraoka, Otaru 047-0264, Japan.
Watanabe
Masato
M
Takechi
Shigeru
S
Kakinoki
Shigeo
S
Nomura
Akikazu
A
D000959:Antihypertensive Agents / Q000097:blood*; D002138:Calibration; D002227:Carbazoles / Q000097:blood*; D000077261:Carvedilol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D011412:Propanolamines / Q000097:blood*; D012680:Sensitivity and Specificity
D000959:Antihypertensive Agents; D002227:Carbazoles; D011412:Propanolamines; D000077261:Carvedilol