[Amplification of beta-catenin gene in the paraffin-embedded epidermal tissue].
OBJECTIVE
To establish a method of extracting DNA from the paraffin-embedded epidermal tissue and examine the amplification of beta-catenin gene by polymerase chain reaction (PCR) analysis.


METHODS
Exon 3 of the beta-catenin gene was amplified by PCR in 14 paraffin-embedded Bowen's disease (BD) of various weight and years.


RESULTS
The amplification of exon 3 of beta-catenin gene was successful in all the 10 mg and 20 mg samples, but not in the 5 mg samples. The amplification of exon 3 of beta-catenin gene was successful in 9 samples embedded within 10 years, and in only 2 of the 5 samples embedded more than 10 years.


CONCLUSIONS
Epidermal tissue whose weight is 10 mg or more within 10 years of embedding can be used in the amplification of purpose gene by PCR.
Chen
Xiang
X
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xie
Hong-fu
HF
Chen
Fu-wen
FW
D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D015534:Trans-Activators; D051176:beta Catenin