Haptoglobin release by human adipose tissue in primary culture.
Haptoglobin is a putative adiposity marker because its concentration in blood is increased in obese humans. The present studies examined haptoglobin release by explants of adipose tissue in primary culture. Haptoglobin was released by explants of human visceral and subcutaneous adipose tissue at a nearly linear rate over 48 h. Explants of visceral adipose tissue released more haptoglobin than did explants of subcutaneous adipose tissue. The release of haptoglobin was quite variable, but there was a close correlation between haptoglobin release by visceral adipose tissue and that by explants of subcutaneous tissue from the same individual. Dexamethasone and niflumic acid, a cyclooxygenase-2 inhibitor, both inhibited haptoglobin release. There was release of haptoglobin by both isolated adipocytes and the adipose tissue matrix remaining after collagenase digestion of human adipose tissue. However, the amount of haptoglobin released by human adipose tissue explants in primary culture was quite low in relationship to the circulating level of haptoglobin.
Fain
John N
JN
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA. jfain@utmem.edu
Bahouth
Suleiman W
SW
Madan
Atul K
AK
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015992:Body Mass Index; D002478:Cells, Cultured; D046508:Culture Techniques; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009544:Niflumic Acid / Q000494:pharmacology; D009765:Obesity / Q000378:metabolism; D040521:Subcutaneous Tissue / Q000378:metabolism
D006242:Haptoglobins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009544:Niflumic Acid; D003907:Dexamethasone