A new 9-lipoxygenase cDNA from developing rice seeds.
We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.
Mizuno
Kouichi
K
Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. koumno@akita-pu.ac.jp
Iida
Toshii
T
Takano
Atsushi
A
Yokoyama
Mineyuki
M
Fujimura
Tatsuhito
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D008084:Lipoxygenase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D012639:Seeds / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D008084:Lipoxygenase