title: GLUT9 is differentially expressed and targeted in the preimplantation embryo.
abstract: During preimplantation development in the mouse, it is crucial that glucose metabolism not be compromised. Any decrease in glucose uptake at this stage in development can compromise the developing embryo. We have cloned another member of the glucose transporter family, GLUT9, which is expressed embryonically. Three different isoforms were identified. We have shown that two of the mouse GLUT9 isoforms transport glucose at a rate significantly greater than controls. Expression analysis of the preimplantation blastocyst identifies only the presence of the shorter GLUT9 isoform, RT-PCR and Western immunoblot confirmed this finding. A differential pattern of expression was seen with GLUT9 present at the plasma membrane in one- and two-cell zygotes and in an intracellular compartment in trophectoderm cells at a blastocyst stage. Although blocking GLUT9 expression during preimplantation development had no effect on glucose transport or apoptosis, transfer of these embryos into pseudopregnant mice resulted in increased pregnancy loss, suggesting that GLUT9 is critical for early preimplantation development.
authors.affiliation: Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110, USA carayannopm@msnotes.wustl.edu
authors.forename: Mary O
authors.initials: MO
authors.lastname: Carayannopoulos
authors.forename: Amanda
authors.initials: A
authors.lastname: Schlein
authors.forename: Amanda
authors.initials: A
authors.lastname: Wyman
authors.forename: Maggie
authors.initials: M
authors.lastname: Chi
authors.forename: Chithra
authors.initials: C
authors.lastname: Keembiyehetty
authors.forename: Kelle H
authors.initials: KH
authors.lastname: Moley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; C498775:Slc2a9 protein, mouse; D005947:Glucose