title: [The experimental study of cryopreserved osteoblasts combined with BGC repairing mandibular defects in rabbits].
abstract: OBJECTIVE
  The purpose of this study was to observe the biological characteristics of cultured Periosteal-derived Osteoblasts (POBs) preserved in liquid nitrogen in vitro, and to preliminarily study the osteogenetic capability of bioactive glass ceramic (BGC) combined with POBs.
  
  
  METHODS
  The cryopreserved cells were cultured in DMEM medium and examined by morphological and histological assay. The POBs growing well in vitro were seeded into the porous BGC materials. A week later, the combined materials were implanted into the bone defects of rabbits' mandibular, the control groups were implanted into the single BGC (no cells). After 2, 4, 8, 12 weeks of operation, the specimens were respectively excised and examined by X-ray and histological chemistry.
  
  
  RESULTS
  The cryopreserved POBs grew well in vitro and also had the tipical characteristics of mature osteoblasts. Cultured with BGC materials, the cells could attach, grow and proliferate well on the surface of most endoporous. After 4 weeks of operation, the transplanted osteoblasts began to form new osteoid or bone tissue in most pores of implanted BGC, and the bone defects were repaired better and earlier.
  
  
  CONCLUSIONS
  It was practical to use the cryopreserved osteoblasts for further study on bone tissue engineering. It suggested that the "life active" bone would get more application and play a more important role in bone restoration and reconstruction.
authors.affiliation: QiLu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Feng-cai
authors.initials: FC
authors.lastname: Wei
authors.forename: Wan-ye
authors.initials: WY
authors.lastname: Tan
authors.forename: Shan-zhen
authors.initials: SZ
authors.lastname: Sun
authors.forename: Guo-ju
authors.initials: GJ
authors.lastname: Li
authors.forename: Huai-shui
authors.initials: HS
authors.lastname: Hou
authors.forename: Bo-long
authors.initials: BL
authors.lastname: Ma
mesh_terms: D000818:Animals; D002516:Ceramics; D015925:Cryopreservation; D005898:Glass; D008334:Mandible / Q000601:surgery*; D010006:Osteoblasts / Q000502:physiology / Q000637:transplantation*; D011817:Rabbits; D023822:Tissue Engineering