Identification of a S100 calcium-binding protein expressed in HL-60 cells treated with all-trans retinoic acid by two-dimensional electrophoresis and mass spectrometry.
To characterize the alteration of protein expression during tumor cell differentiation induced by all-trans retinoic acid (ATRA) and to understand downstream signaling and molecular mechanism of ATRA action, we compared the protein expression profiles in HL-60 cells with ATRA treatment using two-dimensional electrophoresis (2-DE). Although many changes in protein expression were found in 2-DE maps, here we identified two protein spots remarkably expressed in the differentiated cells by nanoelectrospray ionization mass spectrometry and database searching. These two protein spots were found to be the same protein, namely S100 calcium-binding protein A9 (S100A9). Further study will be done to ascertain whether S100A9 plays a role in the regulation of differentiation or just a consequence of differentiation.
Wu
Yuzhang
Y
Institute of Immunology, Third Military Medical University, 30 Gaotanyan, Chongqing 400038, PR China.
Mou
Zhirong
Z
Li
Jintao
J
Zhou
Wei
W
Wei
Bing
B
Zou
Linyun
L
D040502:Calgranulin B / Q000096:biosynthesis / Q000187:drug effects / Q000302:isolation & purification*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D014212:Tretinoin / Q000494:pharmacology*
D040502:Calgranulin B; D011506:Proteins; D014212:Tretinoin