Determination of bopindolol using the flow injection technique coupled with solid phase extraction.
In the proposed procedure, the determination of bopindolol using a flow injection analysis (FIA) technique, with spectrophotometric detection at 635 nm, is described. The method is based on the production of a green, water-soluble complex with ferric ions in acid medium. The automated lab-made FIA system was used for the direct determination of bopindolol in tablets. Bopindolol was adsorbed onto the solid phase in a mini-column, which was integrated directly into the flow system. The positive feature of the use of solid phase extraction (SPE) was the pre-concentration of bopindolol (seven times). The sample throughput was 50 samples per hour. Using the SPE method, bopindolol was determined with a linear range from 125 to 1000 microg ml(-1) (Relative standard deviation (R.S.D.)=1.87%), with a detection limit (3sigma) of 70 microg ml(-1). The method was applied to the determination of bopindolol in Sandonorm tablets. The results obtained were compared with a conventional HPLC method, both analytical techniques were in good agreement.
Meireles
Joana
J
CEQUP/Department of Physical Chemistry, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha 164, 4050, Porto, Portugal.
Sklenárová
Hana
H
Satínský
Dalibor
D
Solich
Petr
P
Araújo
Alberto N
AN
Montenegro
Maria Conceição B S M
MC
D002138:Calibration; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D010869:Pindolol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D013607:Tablets
D013607:Tablets; D010869:Pindolol; C036007:bopindolol