title: Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.
abstract: Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.
authors.affiliation: Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
authors.forename: Holger
authors.initials: H
authors.lastname: Mayta
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: Fanny
authors.initials: F
authors.lastname: Arenas
authors.forename: Teresa
authors.initials: T
authors.lastname: Valencia
authors.forename: Luz
authors.initials: L
authors.lastname: Caviedes
authors.forename: Sonia H
authors.initials: SH
authors.lastname: Montenegro
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ticona
authors.forename: Jaime
authors.initials: J
authors.lastname: Ortiz
authors.forename: Rosa
authors.initials: R
authors.lastname: Chumpitaz
authors.forename: Carlton A
authors.initials: CA
authors.lastname: Evans
authors.forename: Diana L
authors.initials: DL
authors.lastname: Williams
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology / Q000627:therapeutic use; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010568:Peru; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000188:drug therapy
chemical_list: D000995:Antitubercular Agents; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin