Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.
Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a "gold standard") applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.
Segura-Alvarez
Monserrat
M
Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, D-16868 Wusterhausen. Bundesinstitut für Risikobewertung, D-06846 Dessau, Germany.
Richter
Heinz
H
Conraths
Franz J
FJ
Geue
Lutz
L
D000818:Animals; D002417:Cattle; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods; D022621:Shiga Toxin / Q000032:analysis / Q000235:genetics* / Q000633:toxicity; D014709:Vero Cells
D022621:Shiga Toxin