title: Heparin in vitro sensitivity of the activated partial thromboplastin time in canine plasma depends on reagent.
abstract: The in vitro heparin sensitivity of 6 different commercial activated partial thromboplastin time (APTT) reagents was investigated based on artificial plasma samples prepared by addition of sodium heparin at different activities (0-1.5 IU/ml) to pooled normal canine plasma. Statistical analysis using 2-way analysis of variance was based on APTT ratios (APTT/mean APTT control). Significant differences between the APTT ratios of different APTT reagents (P < 0.00001) were found, which also depended on heparin activity (interaction between the factors; P < 0.00001). For example, mean APTT ratio at 0.7 IU/ml heparin varied between 1.2 and 2.5. The results of this study indicate that recommendations for the control of heparin therapy in dogs by APTT ratio should be reagent specific.
authors.affiliation: Clinic for Small Animals, School of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Mischke
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D004283:Dog Diseases / Q000188:drug therapy; D004285:Dogs; D006493:Heparin / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D010314:Partial Thromboplastin Time / Q000662:veterinary; D015203:Reproducibility of Results; D013048:Specimen Handling
chemical_list: D000925:Anticoagulants; D007202:Indicators and Reagents; D006493:Heparin