Glycosyl phenylthiosulfonates (glyco-PTS): novel reagents for glycoprotein synthesis.
Controlled site-selective glycosylation can be achieved by combining site-directed cysteine mutagenesis with chemical modification of the introduced thiol; a new class of more efficient chemoselective reagents, glycosyl phenylthiosulfonates, allow rapid glycosylations of representative simple thiols, peptides and proteins.
Gamblin
David P
DP
Dyson Perrins Laboratory, University of Oxford, South Parks Road, Oxford, UK OX1 3QY.
Garnier
Philippe
P
Ward
Sarah J
SJ
Oldham
Neil J
NJ
Fairbanks
Antony J
AJ
Davis
Benjamin G
BG
D006023:Glycoproteins / Q000138:chemical synthesis*; D007202:Indicators and Reagents / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013886:Thiosulfonic Acids / Q000737:chemistry*
D006023:Glycoproteins; D007202:Indicators and Reagents; D013886:Thiosulfonic Acids