Detection of apoptosis and phagocytosis in vitro in C6 rat glioma cells treated with tiazofurin.
Tiazofurin, an anticancer drug which inhibits IMP dehydrogenase activity and decreases GTP concentration in various malignant cells, induced inhibition of growth and apoptosis in C6 rat glioma in vitro. The effects of tiazofurin were significantly blocked by addition of exogenous guanosine, suggesting the role of decreased GTP in triggering specific signal transduction pathways involved in apoptosis of C6 cells. The most interesting result of this study was the evidence of phagocytosis of apoptotic cells in vitro by neighbouring cells, a phenomenon considered to occur only in apoptosis in vivo. The possibility of observing phagocytosis in C6 glioma cells suggests that this cell system could be a good model for studying mechanisms of phagocytosis in vitro.
Piperski
V
V
ICN Yugoslavia Institute, Center for Biomedical Research, Pasterova 2, Belgrade, Yugoslavia. marinavr@EUnet.yu
Vracar
M
M
Jokanović
M
M
Stukalov
P
P
Rakić
L
L