Advances in capillary electrophoretically mediated microanalysis.
Recent developments in the use of capillary electrophoretic techniques for the in-line study of enzyme reactions and derivatization protocols are reviewed. The article is divided into two parts: (i) in-line enzyme reactions and (ii) in-line derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e., continuous or transient) of the developed EMMA-based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, according to the functional group that is derivatized.
Van Dyck
Sigrid
S
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Katholieke Universiteit Leuven, Leuven, Belgium.
Kaale
Eliangiringa
E
Nováková
Sona
S
Glatz
Zdenek
Z
Hoogmartens
Jos
J
Van Schepdael
Ann
A
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007202:Indicators and Reagents / Q000032:analysis; D009862:Online Systems / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D005456:Fluorescent Dyes; D007202:Indicators and Reagents