Soluble glucocorticoid-induced TNF receptor (sGITR) induces inflammation in mice.
Glucocorticoid-induced TNF receptor (GITR) was a new member of the TNF/nerve growth factor receptor (TNFR/ NGFR) family and induced in murine T cells by dexamathasone. Recombinant soluble GITR (sGITR) induced an inflammation in peritoneal membrane and changes in spleen after i.p. injection of 3 mg/kg in C57BL/6 mice. Spleen was enlarged and percentage of neutrophils and monocytes were increased. The area of red pulp in spleen was increased, while that of white pulp was decreased after GITR injection. The thickening of membrane and neutrophil infiltration was observed in peritoneal membrane with increased myeloperoxidase activity. At later time, neutrophil infiltration moved to inside the tissue with tissue damage. GITR ligand and GITR were expressed constitutively on the surface of spleen cells and cells from peritoneal fluid. In contrast, no significant change in the spleen and in peritoneal membrane was observed in mice treated with LPS. GITR may play a role in body's inflammatory processes.
Shin
Hyun-Hee
HH
Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Kim
Suk-Gi
SG
Lee
Moo-Hyung
MH
Suh
Jae-Hee
JH
Kwon
Byoung S
BS
Choi
Hye-Seon
HS
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D005434:Flow Cytometry; D053450:Glucocorticoid-Induced TNFR-Related Protein; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology; D007267:Injections; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D012995:Solubility; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D048069:Tumor Necrosis Factors
D002352:Carrier Proteins; D053450:Glucocorticoid-Induced TNFR-Related Protein; D017475:Receptors, Nerve Growth Factor; D018124:Receptors, Tumor Necrosis Factor; C106544:Tnfrsf18 protein, mouse; C478966:Tnfsf18 protein, mouse; D048069:Tumor Necrosis Factors