Purification of glysojanin, an antifungal protein, from the black soybean Glycine soja.
A monomeric protein, with a molecular mass of 25 kDa and an N-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean Glycine soja. The protein, designated glysojanin, demonstrated potent antifungal activity against the fungi Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited HIV-1 reverse transcriptase with an IC50 of 47 micromol/L, [methyl-3H]thymidine incorporation by mouse spleen cells with an IC50 of 175 micromol/L, and translation in the rabbit reticulocyte lysate with an IC50 of 20 micromol/L. Glysojanin was purified using a procedure that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography on Mono S, and gel filtration by fast protein liquid chromatography on Superdex 75.
Ngai
Patrick H K
PH
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., China.
Ng
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D002845:Chromatography; D005658:Fungi / Q000187:drug effects*; D005670:Fusarium / Q000187:drug effects*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D030262:Soybean Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects
D000935:Antifungal Agents; D030262:Soybean Proteins; D054303:HIV Reverse Transcriptase