Quantitative study of HIV-1 Tat peptide and TAR RNA interaction inhibited by poly(allylamine hydrochloride).
The interaction of poly(allylamine hydrochloride) (PAH) with TAR RNA has been studied by quartz crystal microbalance (QCM) cooperating with capillary electrophoresis (CE). Experimental results showed that PAH had high affinity for TAR RNA. In particular, PAH could disrupt the interaction of Tat peptide with TAR RNA, which is critical for HIV-1 virus replication. The approaches described here indicate that they are powerful for studying the binding processes of Tat peptide-TAR RNA and drug-TAR RNA, having great significance for the design of new drug.
Zhao
Hong
H
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, People's Republic of China.
Dai
Dongsheng
D
Li
Jinru
J
Chen
Yi
Y
Jiang
Long
L
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015696:Gene Products, tat / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D011073:Polyamines / Q000737:chemistry*; D011485:Protein Binding / Q000187:drug effects; D021281:Quantitative Structure-Activity Relationship; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein
D015696:Gene Products, tat; D011073:Polyamines; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein; C063994:polyallylamine