[Preparation of acellularized porcine heart valve and seeding of bovine aortic endothelial cells].
OBJECTIVE
To explore the possibility of detergent acellularized porcine heart valve serving as a scaffold for tissue engineering valve.


METHODS
The porcine aortic valves were acellularized by use of trypsin-EDTA. Triton X-100, RNase and DNase treatment. Biomechanical characteristics of fresh valves and acellularized valve were tested; also fresh valves, acellularized valve and valves treated with method of bioprothetic treatment were implanted subcutaneously in rats; frequently seeded with bovine aortic endothelial cells(BAECs), and then cultured for 7 days.


RESULTS
The acellularization procedure resulted in complete removal of the cellular components while the construction of matrix was maintained. The matrix could be successfully seeded with in vitro expanded BAECs, which formed a continuous monolayer on the surface. There is no significant difference of PGI2 secretion of BAECs between cells seeded onto the acellular leaflets and that onto the wells of 24-wells plate (P > 0.05).


CONCLUSIONS
Acellularied porcine aortic valve can be applied as a scaffold to develop tissue engineering heart valve.
Ye
Fu-lin
FL
Department of Thoracic and Cardiac Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200433.
Xu
Zhi-yun
ZY
Zhang
Bao-ren
BR
D000818:Animals; D001021:Aortic Valve / Q000166:cytology; D001698:Biomedical Engineering; D001705:Bioprosthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000637:transplantation*; D005109:Extracellular Matrix; D005988:Glyceric Acids; D006350:Heart Valve Prosthesis; D011474:Prosthesis Design; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous
D005988:Glyceric Acids; C053491:polyglyceric acid