[Study of the protective capacity of scorpion venom Buthus occitanus tunetanus polymerised to glutaraldehyde in mice strains with different haplotypes].
The immune response obtained against the toxic fraction of the scorpion venom Buthus occitanus tunetanus detoxified by polymerisation with glutaraldehyde, was analysed for low inbred mice having different haplotypes: C57BL/6 (H-2b) et BALB/c (H-2d) and the SWISS outbred mouse. This three strains of mice, immunized with the polymeric form of Bot-G50 are able to induce an immune response with bumoral mediation. The anti-polymers antibodies obtained from immunized mice, cross-react with the native Bot-G50 fraction. Indeed, in vitro protection experiments demonstrated that immune sera were neutralizing (between 150 and 235 micrograms of Bot-G50 ml). The in vivo protection assays showed that immunized mice could resist the challenge by high amount of toxic fraction (between 70 and 80 micrograms of Bot-G50). This protection was found to be long-lived, since immunized SWISS mice could resist the challenge by 4 DL50 of the toxic fraction (80 micrograms) six month after the start of the immunized program.
Kaabi
H
H
Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, 13, Place Pasteur, BP 74, 1002 Tunis Belvédère.
Kharrat
R
R
el Ayeb
M
M
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000917:Antibody Formation; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005976:Glutaral / Q000737:chemistry; D006239:Haplotypes / Q000235:genetics; D007114:Immunization / Q000379:methods* / Q000592:standards; D051379:Mice / Q000235:genetics*; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D011108:Polymers / Q000737:chemistry; D065008:Scorpion Stings / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control; D012604:Scorpion Venoms / Q000276:immunology*; D012605:Scorpions / Q000145:classification; D014416:Tunisia / Q000453:epidemiology
D000910:Antibodies, Heterophile; D011108:Polymers; D012604:Scorpion Venoms; D005976:Glutaral