title: A three-tiered approach to differentiate Listeria monocytogenes biofilm-forming abilities.
abstract: The purpose of this research was to develop a system for cultivating and evaluating Listeria monocytogenes biofilms that produces consistent and reliable results. A three-tiered approach was used to evaluate biofilm-forming abilities of three L. monocytogenes strains that were originally associated with listeriosis outbreaks. A L. monocytogenes 'honeycomb' biofilm structure was described. L. monocytogenes strains Scott A and V7 were comparable in developing biofilm network structures and F2365 was less effective in forming biofilm. This three-tiered method can be very useful for further biofilm studies.
authors.affiliation: Department of Food Science and Technology, The Ohio State University, 2015 Fyffe Road, 110 Parker Food Science and Technology Building, Columbus, OH 43210, USA.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Marsh
authors.forename: Hongliang
authors.initials: H
authors.lastname: Luo
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D006801:Humans; D008089:Listeria monocytogenes / Q000166:cytology* / Q000302:isolation & purification / Q000502:physiology*; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology; D008853:Microscopy; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis
chemical_list: D011135:Polysaccharides, Bacterial