[Laminin-binding protein (LBP) as a cellular receptor for the virus of Venezuelan equine encephalomyelitis (VEE): Part 1. A study of the interaction between VEE virus virions and the human recombinant LBP].
The interaction of the VEE virus virions with human LBP was investigated. The affinely purified 43 kDa recombinant LBP (rLBP) of man was found to interact effectively with the VEE virus virions purified in immune enzyme assay. The affinity constant of 43 kDa rLBP with virions was equal to 4.3-4.8 x 10(7) M-1. The rabbit antiviral polyclonal antibodies blocked the interaction of rLBP with the VEE virus virions. According to Western blot, rLBP is capable of interacting with the E1 glycoprotein of the VEE virus, which suggests the presence of a specific epitope of binding with LBP in the surface of the E1-E2 heterodimer of the VEE virus. The results confirm that human LBP could be a receptor for the VEE virus.
Bondarenko
E I
EI
Research Institute for Molecular Biology, Vector State Research Center for Virology and Biotechnology, Kolthovo, Novosibirsk Region, Russia.
Protopopova
E V
EV
Konovalova
S N
SN
Sorokin
A V
AV
Kachko
A V
AV
Surovtsev
I V
IV
Loktev
V B
VB
D000818:Animals; D015153:Blotting, Western; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology / Q000378:metabolism*; D004685:Encephalomyelitis, Venezuelan Equine / Q000821:virology; D000939:Epitopes; D006023:Glycoproteins / Q000378:metabolism; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D006801:Humans; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D014771:Virion / Q000276:immunology / Q000378:metabolism*
D000939:Epitopes; D006023:Glycoproteins; D011498:Protein Precursors; D017474:Receptors, Laminin; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins