Ink-jet printing for micropattern generation of laminin for neuronal adhesion.
In order to achieve defined adhesion and neurite outgrowth, the growth substrate must be patterned in an appropriate way. We utilised ink-jet printing by means of a piezo-based microdispenser to create defined line patterns of a polymer with typical dimensions of 100 microm width on glass, silicon, gold and carbon substrates. Vinnapas, a co-polymer of vinyl acetate and ethylene, was mixed with the extracellular matrix protein laminin to achieve neuronal adhesion on the surface of the patterns. It could be demonstrated that the laminin entrapped in the polymer lines can be recognised by a specific antibody. Adhesion of embryonic chicken forebrain neurones is following the prepared lines, and identity of adhering cells could be shown by neurofilament staining. These findings open the route for the generation of complex small neuronal arrays and for the electrochemical investigation of the obtained neuronal matrix.
Turcu
Florin
F
Anal Chem-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
Tratsk-Nitz
Karla
K
Thanos
Solon
S
Schuhmann
Wolfgang
W
Heiduschka
Peter
P
D000818:Animals; D001672:Biocompatible Materials; D002232:Carbocyanines / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002642:Chick Embryo; D007120:Immunochemistry / Q000379:methods; D007281:Ink; D007797:Laminin / Q000378:metabolism*; D008422:Materials Testing; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D011327:Printing / Q000379:methods*; D016548:Prosencephalon / Q000166:cytology / Q000502:physiology
C035005:3,3'-dihexadecylindocarbocyanine; D001672:Biocompatible Materials; D002232:Carbocyanines; D007797:Laminin; D016900:Neurofilament Proteins