[Gene expression profile changes in human multiple myeloma].
OBJECTIVE
To determine the difference in gene expression between human multiple myeloma (MM) and normal bone marrow.


METHODS
cDNA chip was used to detect the mRNA of mononuclear cell from 10 untreated MM patients and 10 cases of normal bone marrow.


RESULTS
Of the 2,048 genes, we found 566 different expression genes, of which the expression of 237 genes was higher and 329 lower in MM than in the normal bone marrow.


CONCLUSIONS
Many genes may be involved in the pathogenesis of MM. cDNA chip technology is an effective tool in the study of tumor related genes.
Shi
Yi-wu
YW
Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Hu
Wei-xin
WX
Tang
Li-jun
LJ
D000328:Adult; D000368:Aged; D001897:Boronic Acids; D000069286:Bortezomib; D004815:Epidermal Growth Factor / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis / Q000235:genetics; D011719:Pyrazines; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D001897:Boronic Acids; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; C100853:MLF2 protein, human; D009687:Nuclear Proteins; D011719:Pyrazines; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D000069286:Bortezomib; D017027:Protein Tyrosine Phosphatases