title: Application of synthetic peptides to the diagnosis of neurocysticercosis.
abstract: We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
authors.affiliation: Instituto Nacional de Neurología y Neurocirugía, México, DF México. afleury@biomedicas.unam.mx
authors.forename: Agnès
authors.initials: A
authors.lastname: Fleury
authors.forename: Constantino
authors.initials: C
authors.lastname: Beltran
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ferrer
authors.forename: Terésa
authors.initials: T
authors.lastname: Garate
authors.forename: Leslie J S
authors.initials: LJ
authors.lastname: Harrison
authors.forename: R Michael E
authors.initials: RM
authors.lastname: Parkhouse
authors.forename: Esperanza
authors.initials: E
authors.lastname: Garcia
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.forename: Julia
authors.initials: J
authors.lastname: Costa-Cruz
authors.forename: Germano
authors.initials: G
authors.lastname: Biondi
authors.forename: Svetlana
authors.initials: S
authors.lastname: Agapejev
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000276:immunology*; D018509:DNA, Helminth / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015723:Gene Library; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020019:Neurocysticercosis / Q000175:diagnosis*; D010446:Peptide Fragments / Q000276:immunology*; D012680:Sensitivity and Specificity; D041202:Taenia saginata / Q000235:genetics / Q000276:immunology*; D041201:Taenia solium / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D010446:Peptide Fragments