Isolation and properties of major components of Penicillium canescens extracellular enzyme complex.
The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.
Sinitsyna
O A
OA
Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia. oasinitsyna@enzyme.chem.msu.ru
Bukhtoyarov
F E
FE
Gusakov
A V
AV
Okunev
O N
ON
Bekkarevitch
A O
AO
Vinetsky
Yu P
YP
Sinitsyn
A P
AP
D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D010407:Penicillium / Q000201:enzymology*; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000737:chemistry / Q000302:isolation & purification*
D005656:Fungal Proteins; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; C019713:alpha-N-arabinofuranosidase; D044644:Endo-1,3(4)-beta-Glucanase