title: Potent induction of apoptosis by germacranolide sesquiterpene lactones on human myeloid leukemia cells.
abstract: This paper studies the cytotoxic effect induced by four known natural sesquiterpene lactones (tatridin A, tamirin, reynosin, ineupatorolide A) and one synthetic derivative (tatridin A diacetate) on the myeloid leukemia cell lines HL-60 and U937. Tatridin A diacetate and ineupatorolide A were found to be the most cytotoxic compounds with growth inhibition caused by induction of apoptosis as determined by flow cytometry and microscopy of nuclear changes. The results reported here support the conclusion that apoptosis was accompanied by both the activation of caspase-3 and the fragmentation of poly(ADP-ribose) polymerase-1 and was also associated with an early release of cytochrome c from the mitochondria.
authors.affiliation: Department of Chemistry, University of Las Palmas de Gran Canaria, Edificio Ciencias Básicas, Campus Universitario de Tafira, 35017 Las Palmas de Gran Canaria, Spain.
authors.forename: Argimiro
authors.initials: A
authors.lastname: Rivero
authors.forename: José
authors.initials: J
authors.lastname: Quintana
authors.forename: José Luis
authors.initials: JL
authors.lastname: Eiroa
authors.forename: Mariana
authors.initials: M
authors.lastname: López
authors.forename: Jorge
authors.initials: J
authors.lastname: Triana
authors.forename: Jaime
authors.initials: J
authors.lastname: Bermejo
authors.forename: Francisco
authors.initials: F
authors.lastname: Estévez
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D045304:Cytochromes c / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D045788:Sesquiterpenes, Germacrane / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D045788:Sesquiterpenes, Germacrane; C432449:germacranolide; D045304:Cytochromes c