title: Direct and distinguishable inhibitory roles for SUMO isoforms in the control of transcriptional synergy.
abstract: Functional interactions between factors bound at multiple sites on DNA often lead to a synergistic or more-than-additive transcriptional response. We previously defined a class of peptide sequences termed synergy control motifs (SC motifs) that function in multiple regulators by selectively inhibiting synergistic activity driven from multiple but not single response elements. By studying the prototypic SC motifs of the glucocorticoid receptor, we show that SC motifs inhibit transcription per se both in cis and in trans, and that a requirement for multiple contacts with DNA renders them selective for compound response elements. Notably, SC motifs are sites for SUMOylation, and the degree of modification correlates strongly with the extent of synergy control. Recruiting SUMO to the promoter either independently or as a fusion to the glucocorticoid receptor is sufficient to recapitulate the in trans and in cis inhibition by SC motifs without apparent changes in subcellular localization. Moreover, we find that the core ubiquitin fold domain of SUMO is sufficient for inhibition and that, independently of their potential for polySUMO chain formation, SUMO-2 and SUMO-3 are more effective inhibitors than SUMO-1.
authors.affiliation: Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109-0632, USA.
authors.forename: Sam
authors.initials: S
authors.lastname: Holmstrom
authors.forename: Mary E
authors.initials: ME
authors.lastname: Van Antwerp
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Iñiguez-Lluhi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D017930:Genes, Reporter; D007700:Kinetics; D008156:Luciferases / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D014162:Transfection
chemical_list: D029721:Drosophila Proteins; D020033:Protein Isoforms; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D004247:DNA; D000426:Alcohol Dehydrogenase; D008156:Luciferases