Application of genome-wide expression analysis to identify molecular markers useful in monitoring industrial fermentations.
Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae identified the YOR387c and YGL258w homologues as highly inducible in zinc-depleted conditions. Induction was specific for zinc deficiency and was dependent on Zap1p. The results indicate that these sequences may be valuable molecular markers for detecting zinc deficiency in industrial fermentations.
Higgins
Vincent J
VJ
Clive and Vera Ramaciotti Centre for Gene Function Analysis, University of New South Wales, Sydney, New South Wales 2052, Australia. v.higgins@unsw.edu.au
Rogers
Peter J
PJ
Dawes
Ian W
IW
D003470:Culture Media; D005285:Fermentation; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005819:Genetic Markers; D016681:Genome, Fungal; D007218:Industrial Microbiology / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D003470:Culture Media; D005819:Genetic Markers; D029701:Saccharomyces cerevisiae Proteins; D015032:Zinc