[cDNAs cloning of SARS-CoV PUMC2 viral genome].
OBJECTIVE
To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.


METHODS
Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.


RESULTS
The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.


CONCLUSIONS
These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
Fan
Zheng
Z
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Tan
Xin-yu
XY
Yin
Bin
B
Zou
Ke
K
Wang
Ting
T
Shen
Yan
Y
Ni
An-ping
AP
Qin
Chuan
C
Yuan
Jian-gang
JG
Qiang
Bo-qin
BQ
Peng
Xiao-zhong
XZ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins