Cysteamine-a somatostatin-inhibiting agent-induced growth hormone secretion and growth acceleration in juvenile grass carp (Ctenopharyngodon idellus).
Effects of cysteamine hydrochloride (CSH)-a somatostatin-inhibiting agent on growth hormone (GH) secretion from pituitary fragments (PF) or hypothalamus plus pituitary fragments (HPF) under static incubation conditions, serum GH, 3,5,3(')-triiodothyronine (T(3)) and thyroxine (T(4)) levels, and growth in juvenile grass carp (Ctenopharyngodon idellus) were investigated. CSH (0.1, 1, and 10 mM) had no influences on GH release from PF after 1 and 6h incubation, but was effective in stimulating GH release from HPF in a dose-dependent manner after 1 and 6h incubation. Moreover, prolonged treatment of HPF with CSH decreased the magnitude of enhancement of GH levels in culture medium. CSH and neuropeptides [e.g., human GH-releasing hormone (hGHRH, 100 nM), luteinizing hormone-releasing hormone analog (LHRH-A, [D-Trp(6),Pro(9)]LHRH, 100 nM)], or salmon gonadotropin-releasing hormone analogue (sGnRH-A, [D-Ala(6),Pro(9)]LHRH, 100 nM), alone and in combination during static incubation stimulated GH release from HPF after 1h incubation; in addition, there was an additive, not a synergistic effect of CSH and neuropeptides on stimulation of GH release. Administration of CSH (2.5mg/g diet) in combination with LHRH-A (5 microg/g diet) in diet twice daily for 8 weeks resulted in higher serum GH, T(3), and T(4) levels, ratio of RNA/DNA in muscle, food conversion efficiency, and growth rate than CSH or LHRH-A alone. At trial termination, significant decreases in condition factors and body lipid levels were observed in fish fed with CSH and/or LHRH-A. No significant differences were recorded for viscero-somatic index, hepato-somatic index, and percent body moisture and protein in muscle. These findings, taken as a whole, strongly suggest that the action of CSH stimulating GH release in vitro appears to be mediated through hypothalamic pathways and dietary delivery of CSH directly or indirectly stimulates endogenous GH, T(3), and T(4) secretion, and subsequently leads to a increase in growth rate in grass carp.
Xiao
Dong
D
Institute of Aquatic Economic Animals and Key Laboratory of Guangdong Province for Aquatic Economic Animals, Zhongshan University, 510275 Guangzhou, PR China. xiao_d@hotmail.com
Lin
Hao Ran
HR
D000284:Administration, Oral; D000818:Animals; D002347:Carps / Q000235:genetics* / Q000502:physiology; D003543:Cysteamine / Q000008:administration & dosage / Q000494:pharmacology*; D013006:Growth Hormone / Q000378:metabolism* / Q000494:pharmacology; D007031:Hypothalamus / Q000502:physiology; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013963:Thyroid Hormones / Q000494:pharmacology
D011837:Radiation-Protective Agents; D013963:Thyroid Hormones; D003543:Cysteamine; D013006:Growth Hormone