title: Alkaloid production in Crinum moorei cultures.
abstract: The alkaloids cherylline (1), crinamidine (2), crinine (3), epibuphanisine (4), lycorine (5), powelline (6), undulatine (7), 1-epideacetylbowdensine (8), and 3-O-acetylhamayne (9) were identified in the in vitro propagated bulblets of Crinum moorei. In addition, crinine, powelline, and undulatine were detected in the solidified Murashige and Skoog (MS) medium. The identity of the alkaloids was confirmed by comparing retention times and mass spectra with known samples. Light, as well as benzyladenine (BA) and charcoal supplementation of the tissue culture medium, influenced the levels of specific alkaloids in both the bulblets and media.
authors.affiliation: Research Centre for Plant Growth and Development, School of Botany and Zoology, Faculty of Science and Agriculture, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa. fennel@nu.ac.za
authors.forename: C W
authors.initials: CW
authors.lastname: Fennell
authors.forename: E E
authors.initials: EE
authors.lastname: Elgorashi
authors.forename: J
authors.initials: J
authors.lastname: van Staden
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D047151:Amaryllidaceae Alkaloids; D031407:Crinum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D015394:Molecular Structure; D010617:Phenanthridines / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D013019:South Africa
chemical_list: D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D007546:Isoquinolines; D010617:Phenanthridines; C424145:cherylline; C015330:lycorine