Additive effect of p53, p21 and Rb deletion in triple knockout primary hepatocytes.
Using Cre-Lox technology to inducibly delete Rb from wild-type, p21- and/or p53-deficient primary hepatocytes, we investigated the role of p53, p21 and pRb in the regulation of liver cell proliferation, polyploidization and death. These cellular decisions are critical to maintaining liver cell replacement in disease, and in determining the likelihood of carcinogenesis in chronic liver injury. Clearly, the present study shows a complex interplay between p53, p21 and pRb, which regulates the likelihood of hepatocytes stimulated from quiescence, to proliferate, undergo polyploidy or die. It reveals that these proteins act both in concert and independently, demonstrating that a small set of key cellular players is common to diverse cell decisions of fundamental importance to disease.
Sheahan
Sharon
S
School of Molecular & Clinical Medicine, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK.
Bellamy
Christopher O C
CO
Treanor
Louise
L
Harrison
David J
DJ
Prost
Sandrine
S
D000818:Animals; D017209:Apoptosis; D002455:Cell Division; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D005434:Flow Cytometry; D017353:Gene Deletion; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008938:Mitosis; D008954:Models, Biological; D011123:Polyploidy; D016160:Retinoblastoma Protein / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics* / Q000502:physiology
C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53