title: [Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells].
abstract: OBJECTIVE
  To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.
  
  
  METHODS
  With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank.
  
  
  RESULTS
  593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.
  
  
  CONCLUSIONS
  Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.
authors.affiliation: Department of Stem Cell Biology, Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.forename: Ci-xian
authors.initials: CX
authors.lastname: Bai
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Pei
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization
chemical_list: D018952:Antigens, CD34