title: Nadroparine inhibits the hypersensitivity response in the conjunctiva.
abstract: This study sought to investigate the effects of nadroparine on an in vivo experimental model of type I hypersensitivity response in the rat conjunctiva. Following drug application onto the eye, either before or after challenge with the mast cell degranulator, basic polyamine compound 48/80, the conjunctival histamine content and the nitrite levels in the conjunctival lavage fluid were quantified fluorometrically and spectrophotometrically, respectively. Instillation into the eye of nadroparine inhibited the C48/80-induced decreases in conjunctival histamine and the delayed increases in nitrite levels, without influencing basal mediator levels. Protamine did not induce histamine release and only partially reversed the effects of nadroparine post-challenge, yet it had no effect on the protective action of the drug when administered prior to degranulation. The results showed that nadroparine was equally effective in attenuating the effects of compound 48/80 in the eye when administered topically either before or after challenge.
authors.affiliation: Department of Experimental Pharmacology, Medical School, University of Athens, M. Asias 75, GR-11527 Athens, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Giannoulaki
authors.forename: Miltiades
authors.initials: M
authors.lastname: Papathanassiou
authors.forename: Nikolaos M
authors.initials: NM
authors.lastname: Sitaras
authors.forename: Ekaterini
authors.initials: E
authors.lastname: Tiligada
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003228:Conjunctiva / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000517:prevention & control*; D007322:Instillation, Drug; D008297:Male; D017762:Nadroparin / Q000494:pharmacology*; D009573:Nitrites / Q000378:metabolism; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D007507:Therapeutic Irrigation; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D017762:Nadroparin; D009573:Nitrites; D011479:Protamines; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine