Using PC12 cells to evaluate poly(caprolactone) and collagenous microcarriers for applications in nerve guide fabrication.
Tissue-engineered nerve guides can provide mechanical support as well as chemical stimulation for nerve regeneration. PC12 cells were used to test the novel combination of poly(caprolactone) (PCL) and macroporous collagen-based microcarriers (CultiSphers) as an initial phase in the fabrication of multichanneled nerve guides. Laminin-coated PCL was an effective matrix for the attachment, proliferation, and viability of PC12 cells, relative to uncoated PCL. PC12 cells attached to laminin-coated PCL and extended neurites when cultured in the presence of nerve growth factor (NGF). PC12 cells attached and proliferated on CultiSphers and extended neurites in response to NGF. A novel PCL/CultiSpher composite material also supported PC12 attachment and proliferation and provides a potentially useful material for the fabrication of synthetic nerve guides.
Waddell
Rebecca L
RL
Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.
Marra
Kacey G
KG
Collins
Kelly L
KL
Leung
Jade T
JT
Doctor
John S
JS
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D003094:Collagen / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D008863:Microspheres; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D016716:PC12 Cells; D019736:Prostheses and Implants; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D002208:Caproates; D007783:Lactones; C121056:caprolactone; D003094:Collagen