First estimation of C4-H bond dissociation energies of NADH and its radical cation in aqueous solution.
The heterolytic and homolytic C4-H bond dissociation energies of NADH and its radical cation (NADH*+) in aqueous solution were estimated according to the reaction of NADH with N,N,N',N'-tetramethyl-p-phenylenediamine radical cation perchlorate (TMPA*+) in aqueous solution. The results show that the values of the heterolytic and homolytic C4-H bond dissociation energies of NADH in aqueous solution are 53.6 and 79.3 kcal/mol, respectively; the values of the heterolytic and homolytic C4-H bond dissociation energies of NADH*+*+ in aqueous solution are 5.1 and 36.3 kcal/mol, respectively, which, to our knowledge, is first reported. This energetic information disclosed in the present work should be believed to furnish hints to the understanding of the mechanisms for the redox interconversions of coenzyme couple NADH/NAD+ in vivo.
Zhu
Xiao-Qing
XQ
Department of Chemistry, National Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xqzhu@nankai.edu.cn
Yang
Yuan
Y
Zhang
Min
M
Cheng
Jin-Pei
JP
Zhang
Ming
M
D002412:Cations / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D009243:NAD / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013772:Tetramethylphenylenediamine / Q000737:chemistry; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D002412:Cations; D005609:Free Radicals; D012996:Solutions; D014867:Water; D009243:NAD; D013772:Tetramethylphenylenediamine