title: Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-gamma coactivator-1 and peroxisome proliferator-activated receptor-alpha in skeletal muscle.
abstract: The peroxisome proliferator-activated receptor (PPAR)-gamma coactivator-1 (PGC-1) can induce mitochondria biogenesis and has been implicated in the development of oxidative type I muscle fibers. The PPAR isoforms alpha, beta/delta, and gamma control the transcription of genes involved in fatty acid and glucose metabolism. As endurance training increases skeletal muscle mitochondria and type I fiber content and fatty acid oxidative capacity, our aim was to determine whether these increases could be mediated by possible effects on PGC-1 or PPAR-alpha, -beta/delta, and -gamma. Seven healthy men performed 6 weeks of endurance training and the expression levels of PGC-1 and PPAR-alpha, -beta/delta, and -gamma mRNA as well as the fiber type distribution of the PGC-1 and PPAR-alpha proteins were measured in biopsies from their vastus lateralis muscle. PGC-1 and PPAR-alpha mRNA expression increased by 2.7- and 2.2-fold (P < 0.01), respectively, after endurance training. PGC-1 expression was 2.2- and 6-fold greater in the type IIa than in the type I and IIx fibers, respectively. It increased by 2.8-fold in the type IIa fibers and by 1.5-fold in both the type I and IIx fibers after endurance training (P < 0.015). PPAR-alpha was 1.9-fold greater in type I than in the II fibers and increased by 3.0-fold and 1.5-fold in these respective fibers after endurance training (P < 0.001). The increases in PGC-1 and PPAR-alpha levels reported in this study may play an important role in the changes in muscle mitochondria content, oxidative phenotype, and sensitivity to insulin known to be induced by endurance training.
authors.affiliation: Clinique romande de réadaptation, SUVA, Sion, Switzerland. Endocrine Unit, University Hospital Geneva, Geneva, Switzerland. aaron.russell@crr-suva.ch
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Russell
authors.forename: Jonas
authors.initials: J
authors.lastname: Feilchenfeldt
authors.forename: Sylvia
authors.initials: S
authors.lastname: Schreiber
authors.forename: Manu
authors.initials: M
authors.lastname: Praz
authors.forename: Antoinette
authors.initials: A
authors.lastname: Crettenand
authors.forename: Charles
authors.initials: C
authors.lastname: Gobelet
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Meier
authors.forename: David R
authors.initials: DR
authors.lastname: Bell
authors.forename: Anastasia
authors.initials: A
authors.lastname: Kralli
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
authors.forename: Olivier
authors.initials: O
authors.lastname: Dériaz
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010806:Physical Education and Training; D010807:Physical Endurance; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1