title: Dietary cysteine reduces the methionine requirement by an equal proportion in both parenterally and enterally fed piglets.
abstract: The sulfur amino acids (SAA), methionine and cysteine, are normally supplied in a 50:50 ratio in the oral diet of pigs. In contrast, cysteine is not included in any appreciable amounts in parenteral solutions due to its instability in solution. Cysteine can replace part of the methionine requirement, but is not required when methionine is supplied at a level that meets the entire SAA requirement. However, the role of the gut on cysteine sparing has not been investigated. In the present study, the enteral and parenteral methionine requirement was determined, with excess dietary cysteine, by using the indicator amino acid oxidation (IAAO) technique. Piglets [n = 28, 2 d, 1.65 +/- 0.014 kg (SE)] were fed elemental diets containing adequate energy, phenylalanine and excess tyrosine, with varied methionine concentrations and excess cysteine [0.55 g/(kg. d)]. Diets were infused continuously via intravenous (parenteral) or gastric (enteral) catheters. Phenylalanine oxidation was determined during a primed, constant infusion of L-[1-(14)C]-phenylalanine, by measuring expired (14)CO(2) and plasma specific radioactivity (SRA) of phenylalanine. For both the parenteral and enteral groups, phenylalanine oxidation (% of dose) decreased linearly (P < 0.01) as methionine intake increased and then became low and unchanging. Using breakpoint analysis, the methionine requirement was estimated to be 0.25 and 0.18 g/(kg. d) for enteral and parenteral feeding, respectively. These data show that the parenteral methionine requirement is approximately 70% of the enteral requirement when measured in the presence of excess dietary cysteine (P < 0.05). A comparison with our previous studies in which methionine was the only source of sulfur amino acids shows that the addition of dietary cysteine reduces the methionine requirement by approximately 40% in both enterally and parenterally fed neonatal piglets. Therefore, dietary cysteine is equally effective in sparing dietary methionine whether fed enterally or parenterally.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Shoveller
authors.forename: Janet A
authors.initials: JA
authors.lastname: Brunton
authors.forename: James D
authors.initials: JD
authors.lastname: House
authors.forename: Paul B
authors.initials: PB
authors.lastname: Pencharz
authors.forename: Ronald O
authors.initials: RO
authors.lastname: Ball
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000097:blood; D003545:Cysteine / Q000008:administration & dosage* / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D004750:Enteral Nutrition; D008715:Methionine / Q000008:administration & dosage*; D009751:Nutritional Requirements; D010288:Parenteral Nutrition; D014176:Protein Biosynthesis; D013552:Swine
chemical_list: D008715:Methionine; D003545:Cysteine