Using two photon microscopy to quantify enzymatic reaction rates on polymer beads.
Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports.
Bosma
Annie Y
AY
School of Chemistry, The University of Edinburgh, Edinburgh, UK EH9 3JJ.
Ulijn
Rein V
RV
McConnell
Gail
G
Girkin
John
J
Halling
Peter J
PJ
Flitsch
Sabine L
SL
D000178:Acrylamides / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D004058:Diffusion; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D017785:Photons; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism*; D000099166:Polyacrylamides
D000178:Acrylamides; D004800:Enzymes, Immobilized; D011092:Polyethylene Glycols; D011108:Polymers; D013820:Thermolysin; C113798:polyethyleneglycol-polyacrylamide copolymer; D000099166:Polyacrylamides