Instrumentation for multi-modal spectroscopic diagnosis of epithelial dysplasia.
Reflectance and fluorescence spectroscopies have shown great promise for early detection of epithelial dysplasia. We have developed a clinical reflectance spectrofluorimeter for multimodal spectroscopic diagnosis of epithelial dysplasia. This clinical instrument, the FastEEM, collects white light reflectance and fluorescence excitation-emission matrices (EEM's) within a fraction of a second. In this paper we describe the FastEEM instrumentation, designed for collection of multi-modal spectroscopic data. We illustrate its performance using tissue phantoms with well defined optical properties and biochemicals of known fluorescence properties. In addition, we discuss our plans to develop a system that combines a multi-spectral imaging device for wide area surveillance with this contact probe device.
Tunnell
J W
JW
G.R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Ave., Bldg 6-014, Cambridge, MA 02139, USA.
Desjardins
A E
AE
Galindo
L
L
Georgakoudi
I
I
McGee
S A
SA
Mirkovic
J
J
Mueller
M G
MG
Nazemi
J
J
Nguyen
F T
FT
Wax
A
A
Zhang
Q
Q
Dasari
R R
RR
Feld
M S
MS
D002277:Carcinoma / Q000175:diagnosis*; D004848:Epithelium / Q000473:pathology*; D005336:Fiber Optic Technology; D006801:Humans; D055095:Optics and Photonics; D019047:Phantoms, Imaging; D012984:Software; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013057:Spectrum Analysis / Q000295:instrumentation*