Cilostazol reduces brain lesion induced by focal cerebral ischemia in rats--an MRI study.
To investigate the effects of cilostazol on the hemispheric ischemic lesion, we monitored the apparent diffusion coefficient (ADC) and T2 images by MRI techniques in comparison with histology at the terminal of and after 24-h reperfusion following 2-h occlusion of middle cerebral artery (MCA). The ADC values of tissue water and T2-weighted images were quantified by high field magnetic resonance. No significant difference was observed by ADC image among vehicle and cilostazol treatment groups when measured during MCA occlusion. Oral treatment with cilostazol 30 mg/kg two times at 5 min and 4 h significantly suppressed the hemispheric lesion area and volumes when detected by ADC, T2 images and histology, but 3 and 10 mg/kg cilostazol were without effect. Cilostazol (30 mg/kg) significantly reduced the increased cerebral water content at the ischemic hemisphere compared with vehicle group. In line with these results, the neurological deteriorations were much improved in the cilostazol-treated group. Taken together, it is concluded that post-treatment with cilostazol exerts a potent protective effect against cerebral infarct size by reducing the cytotoxic edema.
Lee
Jeong Hyun
JH
Department of Pharmacology, College of Medicine, Busan National University, Ami-Dong 1-Ga, Seo-Gu, Pusan 602-739, South Korea.
Lee
Yong-Kyu
YK
Ishikawa
Makoto
M
Koga
Keiko
K
Fukunaga
Mari
M
Miyakoda
Goro
G
Mori
Toyoki
T
Hosokawa
Tetsumi
T
Hong
Ki Whan
KW
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D001929:Brain Edema / Q000188:drug therapy* / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D000077407:Cilostazol; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013777:Tetrazoles / Q000494:pharmacology / Q000627:therapeutic use*
D013777:Tetrazoles; D000077407:Cilostazol