title: Experimental allogenic penile transplantation.
abstract: OBJECTIVE
  The aim of this study was to assess the feasibility of allogenic penile transplantation (PTx) for creating a source of viable penile tissue for use in penile reconstruction.
  
  
  METHODS
  The entire penis from an adult Brown-Norway rat was transplanted into a pouch created in the omentum of an adult Lewis rat (fully allogenic PTx, n = 23). Recipients were divided into 2 groups according to immunosuppressant (FK506) usage: in the FK+ group, FK506 (0.6 mg/kg/d) was administered intraperitoneally until a predetermined day (day 3, 5, 7, 10, 14, or 21) after PTx, and then the grafts were harvested. No FK506 was used in the FK- group. Syngeneic PTx (n = 8) patients were used as controls. All grafts were stained with H&E for histologic examination.
  
  
  RESULTS
  At laparotomy, each successfully transplanted penis appeared as a cylindrical mass in the omentum. Grafts could be mobilized to the genital area because of a long omental pedicle. Graft survival in the control and FK+ groups was 100%. Rejection was minimal to moderate in FK+ grafts harvested on days 3 and 5 after PTx and minimal or absent in FK+ grafts harvested on days 7, 10, 14, and 21. Penile structure on H&E staining was normal in FK+ and control specimens. Rejection with massive cellular infiltration was observed in all FK- grafts.
  
  
  CONCLUSIONS
  FK506 successfully prevented rejection in allogenic PTx, and the authors' technique has potential for creating viable penile tissue that could be used as an option for penile reconstruction.
authors.affiliation: Department of Pediatric Surgery and The Central Laboratory, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koga
authors.forename: Atsuyuki
authors.initials: A
authors.lastname: Yamataka
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kato
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Lane
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Sueyoshi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyano
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D006084:Graft Rejection; D006085:Graft Survival; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008297:Male; D010413:Penis / Q000033:anatomy & histology; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016559:Tacrolimus / Q000627:therapeutic use; D014184:Transplantation, Homologous; D000093667:Penile Transplantation
chemical_list: D007166:Immunosuppressive Agents; D016559:Tacrolimus