Molecular detection and identification of agents of eumycetoma: detailed report of two cases.
We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.
Ahmed
Abdalla O A
AO
Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
Desplaces
Nicole
N
Leonard
Philippe
P
Goldstein
Fred
F
De Hoog
Sybren
S
Verbrugh
Henri
H
van Belkum
Alex
A
D000328:Adult; D000935:Antifungal Agents / Q000627:therapeutic use; D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D017964:Itraconazole / Q000627:therapeutic use; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009181:Mycoses / Q000175:diagnosis* / Q000473:pathology / Q000628:therapy; D016133:Polymerase Chain Reaction / Q000379:methods
D000935:Antifungal Agents; D004271:DNA, Fungal; D017964:Itraconazole