title: [Studies on establishing rRNA gene map of Angelica sinensis and Rheum palmatum from Gansu by DNA sequencing].
abstract: OBJECTIVE
  To amplify and sequence the internal transcripted spacer of rRNA gene of the seeds of Angelica sinensis and Rheum palmatum for establishing a new method to identify the Chinese herb seeds in molecular level.
  
  
  METHODS
  rDNA in the seeds was extracted. Specific synthesized primers were applied to amplify the internal transcripted spacer of rRNA gene by nested PCR. And the PCR products were sequenced.
  
  
  RESULTS
  PCR results were confirmed by agarose electrophoresis, the above-mentioned methods could achieve the PCR products of interval region of rRNA gene in the seeds, and the sequences of the internal transcripted spacer of rRNA gene in the seeds were acquired by sequencing. There existed marked difference.
  
  
  CONCLUSIONS
  The base sequences of the internal transcripted spacer of rRNA gene in different plant Chinese seeds can be applied to be identifying marker in molecular level.
authors.affiliation: Gansu College of Traditional Chinese Medicine, Lanzhou 730000.
authors.forename: Xiling
authors.initials: X
authors.lastname: Zhang
authors.forename: Keping
authors.initials: K
authors.lastname: Ji
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Li
authors.forename: Che
authors.initials: C
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Lisha
authors.initials: L
authors.lastname: Liu
mesh_terms: D029971:Angelica sinensis / Q000235:genetics*; D001483:Base Sequence; D020459:Genes, rRNA; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012250:Rheum / Q000235:genetics*; D012639:Seeds / Q000235:genetics
chemical_list: D005819:Genetic Markers; D018749:RNA, Plant; D012335:RNA, Ribosomal