[Effect of nandrolone phenylpropionate on expression of hepatic albumin-mRNA and androgen receptor in burned rats].
OBJECTIVE
To study the mechanism of nandrolone phenylpropionate (NP) on hepatic albumin mRNA and androgen receptor(AR) in burned rats.


METHODS
Thirty-two Wistar rats with a deep second-degree cutaneous burn of 20% total body surface area were randomly divided into two groups: NP group (experimental group, 5 mg/kg NP) and normal saline as control group every other day. The expression copy quantity of albumin-mRNA and mean integrated absorbency(mIA) of AR in liver tissue were measured by quantitative fluorescent RT-PCR and immunohistochemistry respectively on the 4th, 7th, 14th and 21st days of post-burned.


RESULTS
The expression levels of albumin-mRNA and AR in liver tissue in NP group were much higher than those in control group. The albumin-mRNA and AR expression increased significantly (P < 0.05) after 7 and 14 days, while the expression had no significant difference between NP group and control group on the 4th day. A positive correlation occurred between the expression level of albumin-mRNA and the quantity of AR in liver tissue(r = 0.936, P < 0.05).


CONCLUSIONS
Nandrolone phenylpropionate up-regulated respectively the expression of albumin-mRNA and the density of AR in liver tissue.
Cen
Ying
Y
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Li
Kai
K
Liu
Xiao-xue
XX
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002056:Burns / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000096:biosynthesis* / Q000235:genetics
D000418:Albumins; D012333:RNA, Messenger; D011944:Receptors, Androgen; D009277:Nandrolone; C008580:nandrolone phenpropionate