Identification using LC-MSn of co-metabolites in the biosynthesis of the polyketide toxin mycolactone by a clinical isolate of Mycobacterium ulcerans.
LC-MSn analysis of mycolactone toxin from extracts of Mycobacterium ulcerans has shown that minor co-metabolites, including two previously unreported, differ structurally from mycolactone only in a small portion of the polyketide side-chain.
Hong
Hui
H
Department of Chemistry and Biochemistry, University of Cambridge, Cambridge, UK.
Gates
Paul J
PJ
Staunton
James
J
Stinear
Tim
T
Cole
Stewart T
ST
Leadlay
Peter F
PF
Spencer
Jonathan B
JB
D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D018942:Macrolides; D015394:Molecular Structure; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D019911:Mycobacterium ulcerans / Q000302:isolation & purification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D001427:Bacterial Toxins; D018942:Macrolides; C117218:mycolactone