Evidence for alternative splicing and developmental regulation of the Drosophila melanogaster Mgat2 (N-acetylglucosaminyltransferase II) gene.
We present a molecular study of the Drosophila melanogaster Mgat2 gene that codes for the N-acetylglucosaminyltransferase II. Isolation and analysis of two cDNA clones indicated that the gene is alternatively spliced, generating two transcripts of 3.3 and 2.7kb. Developmental specificity was observed between the two alternative transcripts during the major developmental stages of D. melanogaster. The deduced amino acid sequences of the two proteins show significant homology to the equivalent mammalian proteins, especially in the carboxyterminal region. In situ hybridizations in embryos and embryonic imaginal discs showed that the gene is expressed mainly but not exclusively in the brain.
Tsitilou
Sonia G
SG
Department of Biochemistry and Molecular Biology, University of Athens, 15701, Athens, Greece. tsitilou@biol.uoa.gr
Grammenoudi
Sofia
S
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019154:Protein Splicing / Q000502:physiology*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D017351:N-Acetylglucosaminyltransferases; C063313:alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase