Comparative analysis of Borrelia isolates from southeastern USA based on randomly amplified polymorphic DNA fingerprint and 16S ribosomal gene sequence analyses.
Fifty-three southern USA Borrelia isolates were characterized using randomly amplified polymorphic DNA fingerprinting analysis (RAPD). Twenty-nine types were recognized among 37 B. andersonii strains, seven types among eight B. bissettii strains, and seven types among seven B. burgdorferi sensu stricto strains. Strain TXW-1 formed a separate RAPD type. Nearly complete sequences of the rrs genes from 17 representative southern Borrelia were determined. The similarity values were found to be 96-100% within the B. burgdorferi sensu lato (s.l.) complex, 94-99% among the relapsing fever borreliae, and 93-99% between the two complexes. Phylogenetic analysis indicated that all the Borrelia strains we analyzed could be divided into two parts: the B. burgdorferi s.l. complex and the relapsing fever borreliae complex. TXW-1 segregated with the North American relapsing fever borreliae and formed a separate subbranch.
Lin
Tao
T
Institute of Arthropodology and Parasitology, PO Box 8056, Georgia Southern University, Statesboro, GA 30460-8056, USA.
Oliver
James H
JH
Gao
Lihui
L
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D001898:Borrelia / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis / Q000302:isolation & purification; D004197:Disease Reservoirs; D020459:Genes, rRNA; D005838:Genotype; D008193:Lyme Disease / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012061:Relapsing Fever / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015144:Southeastern United States; D013987:Ticks / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S