title: Intravital microscopy identifies selectins that regulate T cell traffic into allografts.
abstract: T cell homing to sites of injury and inflammation is a critical step for adaptive immune responses. While much has been learned regarding T cell homing to lymphoid tissues, few studies have directly observed trafficking events during an effector response. In this study, we developed a model that uses intravital fluorescence videomicroscopy to determine the molecules critical to T cell rolling within skin allograft microvasculature during the effector phase of the rejection response. Additional studies were performed to quantify T cell infiltrates as rejection progressed. We found that P-selectin and E-selectin expressed on postcapillary venules play overlapping roles in the recruitment of activated T cells in a SCID reconstitution model of skin graft rejection and are important in T cell accumulation at the graft site. Surprisingly, we also found that naive T cells are recruited and accumulate via constitutive T cell L-selectin and upregulated L-selectin ligands on rejecting allograft vasculature. These data indicated that a specific retinue of molecules is upregulated during the rejection response, and they suggest potential future therapeutic targets.
authors.affiliation: Emory Transplant Center and Department of Surgery, Emory Universuty School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Jones
authors.forename: Nozomu
authors.initials: N
authors.lastname: Shirasugi
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Adams
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Pearson
authors.forename: Christian P
authors.initials: CP
authors.lastname: Larsen
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D019040:E-Selectin / Q000502:physiology; D006084:Graft Rejection / Q000276:immunology*; D019041:L-Selectin / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018715:Microscopy, Video; D019007:P-Selectin / Q000502:physiology; D019087:Selectins / Q000502:physiology*; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000502:physiology*; D014184:Transplantation, Homologous
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D019087:Selectins; D019041:L-Selectin