title: Role of arginine 226 in the mechanism of tryptophan indole-lyase from Proteus vulgaris.
abstract: In the spatial structure of tryptophanase from Proteus vulgaris the guanidinium group of arginine 226 forms a salt bridge with the 3;-oxygen atom of the coenzyme. The replacement of arginine 226 with alanine using site-directed mutagenesis reduced the affinity of the coenzyme for the protein by one order of magnitude compared to the wild-type enzyme. The catalytic activity of the mutant enzyme in the reaction with L-tryptophan was reduced 10(5)-fold compared to the wild-type enzyme. The rates of the reactions with some other substrates decreased 10(3)-10(4)-fold. The mutant enzyme catalyzed exchange of the C-alpha-proton in complexes with some inhibitors with rates reduced 10(2)-fold compared to the wild-type enzyme. Absorption and circular dichroism spectra of the mutant enzyme and the enzyme-inhibitor complexes demonstrate that the replacement of arginine 226 with alanine does not significantly affect the tautomeric equilibrium of the internal aldimine, but it leads to an alteration of the optimal conformation of the coenzyme-substrate intermediates.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia. tvd@eimb.ru
authors.forename: V V
authors.initials: VV
authors.lastname: Kulikova
authors.forename: L N
authors.initials: LN
authors.lastname: Zakomirdina
authors.forename: N P
authors.initials: NP
authors.lastname: Bazhulina
authors.forename: I S
authors.initials: IS
authors.lastname: Dementieva
authors.forename: N G
authors.initials: NG
authors.lastname: Faleev
authors.forename: P D
authors.initials: PD
authors.lastname: Gollnick
authors.forename: T V
authors.initials: TV
authors.lastname: Demidkina
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000378:metabolism; D001120:Arginine / Q000737:chemistry; D020134:Catalytic Domain / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D011514:Proteus vulgaris / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D013379:Substrate Specificity; D014368:Tryptophanase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001120:Arginine; D014368:Tryptophanase