Nondestructive analyses of unsaturated fatty acid species in dietary oils by attenuated total reflectance with Fourier transform IR spectroscopy.
The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR absorption bands with various peak positions and intensities at around 3010 cm(-1), depending on the extent of unsaturation and PUFA species. With the aid of partial least-squares regression analysis, the FTIR measurement could practically predict the content of each PUFA species in the oil to be tested. A calculation method was also presented to directly find PUFA species in oils from the FTIR spectra. This technique was applied to dried soybean seeds to demonstrate a nonhomogenous distribution of saturated fatty acids and PUFAs, as well as glycans, in soybean cross sections.
Yoshida
Satoshi
S
Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. xyosida@biomol.gifu-u.ac.jp
Yoshida
Hiroshi
H
D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D005224:Fats, Unsaturated / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
D004042:Dietary Fats, Unsaturated; D005224:Fats, Unsaturated; D005231:Fatty Acids, Unsaturated