Phenotypic and molecular identity of cells in the adult subventricular zone. in vivo and after expansion in vitro.
We have studied the molecular identity of adult mouse SVZ cells in situ, and after isolation and expansion as neurospheres in vitro. The gene and protein expression patterns of the adult cells have been compared to that of the cells from the lateral ganglionic eminence (LGE), their putative embryonic counterparts. The LGE gives rise to both striatal projection neurons and olfactory bulb interneurons via spatially and molecularly distinct progenitor populations present in the SVZ of the LGE. These two populations are thought to have a common origin in the GSH2 expressing cells of the embryonic LGE ventricular zone. We found that a significant number of cells in the adult SVZ, and in the in vitro expanded neurospheres, derived from the adult SVZ express GSH2. However, under normal conditions, GSH2-expressing cells in the adult SVZ and in the in vitro expanded neurospheres appear to specify only olfactory bulb progenitors and not striatal progenitors.
Parmar
Malin
M
Wallenberg Neuroscience Center, Division of Neurobiology, Lund University, BMC A11, SE-221 84 Lund, Sweden. Malin.Parmar@mphy.lu.se
Sjöberg
Andreas
A
Björklund
Anders
A
Kokaia
Zaal
Z
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D010641:Phenotype; D018874:Spheroids, Cellular; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D015415:Biomarkers; D004268:DNA-Binding Proteins; C088334:Distal-less homeobox proteins; C490641:Etv1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; C109498:Gsh2 protein, mouse; D018398:Homeodomain Proteins; C085115:Is1-1 protein; D008667:Metalloproteins; C102641:Mrg1 protein, mouse; D014157:Transcription Factors