A reduced protein model with accurate native-structure identification ability.
A protein model that is simple enough to be used in protein-folding simulations but accurate enough to identify a protein native fold is described. Its geometry consists of describing the residues by one, two, or three pseudoatoms, depending on the residue size. Its energy is given by a pairwise, knowledge-based potential obtained for all the pseudoatoms as a function of their relative distance. The pseudoatomic potential is also a function of the primary chain separation and residue order. The model is tested by gapless threading on a large, representative set of known protein and decoy structures obtained from the "Decoys 'R' Us" database. It is also tested by threading on gapped decoys generated for proteins with many homologs. The gapless threading tests show near 98% native-structure recognition as the lowest energy structure and almost 100% as one of the three lowest energy structures for over 2200 test proteins. In decoy threading tests, the model recognized the majority of the native structures. It is also able to recognize native structures among gapped decoys, in spite of close structural similarities. The results indicate that the pseudoatomic model has native recognition ability similar to comparable atomic-based models but much better than equivalent residue-based models.
Betancourt
Marcos R
MR
University at Buffalo Center of Excellence in Bioinformatics, Buffalo, New York 14203, USA.
D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D008956:Models, Chemical; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D013816:Thermodynamics
D000596:Amino Acids; D011506:Proteins