Preliminary validation of a new standard of efficacy for stored platelets.
BACKGROUND
Platelet preparation and storage systems, unlike those for RBC, lack an objective, absolute performance criterion to determine acceptability. Recently, a criterion based on paired comparison with the radiolabeled recovery and survival of "fresh" platelets has been proposed, namely, recovery = two-thirds and survival = half of "fresh" platelets.


METHODS
Eleven normal subjects donated a unit of leukoreduced apheresis platelets using a standard, approved system. They received an aliquot radiolabeled with 111In or 51Cr (random selection) 4 to 20 hours after donation and, using the other radioisotope, on Day 5 of storage. The recovery was calculated based on the injectate radioactivity. The survival was determined using the multiple-hit model. The area under the platelet survival curve was calculated using the COST program.


RESULTS
Reinfusion of platelets less than 20 hours after collection resulted in a recovery of 74.7 +/- 12.3 percent and a survival time of 7.5 +/- 1.1 days. Reinfusion on Day 5 resulted in a recovery of 58.2 +/- 12.0 percent and a survival time of 6.9 +/- 1.4 days, values that were 77.9 +/- 9.5 percent and 91.8 +/- 16.1 percent of the observation using "fresh" platelets, respectively. The area under the curve using Day 5 platelets was 67.8 +/- 11.5 percent of that using "fresh" platelets.


CONCLUSIONS
The proposed criterion for objective evaluation of platelet preparation and storage systems appears applicable to a commonly accepted approach, leukoreduced apheresis platelets stored in plasma for 5 days, and merits evaluation using other collection, treatment, and storage systems.
AuBuchon
James P
JP
Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA. james.p.aubochon@hitchcock.org
Herschel
Louise
L
Roger
Jill
J
Murphy
Scott
S
D000328:Adult; D019540:Area Under Curve; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation / Q000592:standards*; D002470:Cell Survival; D005260:Female; D006801:Humans; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion; D020127:Recovery of Function; D013997:Time Factors