[DNA analysis of a 500 year mummy sample].
OBJECTIVE
To accumulate experience for dated forensic matter analysis, for example, Mummy.


METHODS
DNA are extracted by methods of phenol-chloroform and are purified by Wizard DNA clean-up system. The STRs locus are ampolification with Promega Powerplus 16 system. The mtDNA hypervariable region 1 (HV1) is amplificated by '3 pair primers'. The products were sequenced with 377 DNA sequencer.


RESULTS
The STRs locus very distinctness and mtDNA sequence is correct.


CONCLUSIONS
It is a valuable method for special forensic matters.
Tong
Da-yue
DY
Faculty of Forensic Medicine, Zhongshan Medical College, Zhongshan University, Guangzhou 510089, China. tddy0@163.com
Wu
Xin-yao
XY
Cai
Gui-qing
GQ
D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005554:Forensic Medicine; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D009106:Mummies; D017422:Sequence Analysis, DNA / Q000379:methods
D004272:DNA, Mitochondrial; D004247:DNA