title: [Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx].
abstract: In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.
authors.affiliation: Cancer Research Institute, Xiangya Medical School of Central South University, Changsha 410078, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Lan
authors.forename: Xiang-Ling
authors.initials: XL
authors.lastname: Feng
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Qiao
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Shen
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009305:Nasopharynx / Q000737:chemistry / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013270:Stomach / Q000737:chemistry; D014059:Tongue / Q000737:chemistry / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins