title: Dihydropyridine and ryanodine receptor binding after eccentric contractions in mouse skeletal muscle.
abstract: The purpose of this study was to determine whether there are alterations in the dihydropyridine and/or ryanodine receptors that might explain the excitation-contraction uncoupling associated with eccentric contraction-induced skeletal muscle injury. The left anterior crural muscles (i.e., tibialis anterior, extensor digitorum longus, and extensor hallucis longus) of mice were injured in vivo by 150 eccentric contractions. Peak isometric tetanic torque of the anterior crural muscles was reduced approximately 45% immediately and 3 days after the eccentric contractions. Partial restoration of peak isometric tetanic and subtetanic forces of injured extensor digitorum longus muscles by 10 mM caffeine indicated the presence of excitation-contraction uncoupling. Scatchard analysis of [3H]ryanodine binding indicated that the number of ryanodine receptor binding sites was not altered immediately postinjury but decreased 16% 3 days later. Dihydropyridine receptor binding sites increased approximately 20% immediately after and were elevated to the same extent 3 days after the injury protocol. Muscle injury did not alter the sensitivity of either receptor. These data suggest that a loss or altered sensitivity of the dihydropyridine and ryanodine receptors does not contribute to the excitation-contraction uncoupling immediately after contraction-induced muscle injury. We also concluded that the loss in ryanodine receptors 3 days after injury is not the primary cause of excitation-contraction uncoupling at that time.
authors.affiliation: Muscle Biology Laboratory, Department of Kinesiology and Health, Georgia State University, Atlanta, GA 30303, USA. cingalls@gsu.edu
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Ingalls
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Warren
authors.forename: Jia-Zheng
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Susan L
authors.initials: SL
authors.lastname: Hamilton
authors.forename: R B
authors.initials: RB
authors.lastname: Armstrong
mesh_terms: D000818:Animals; D001665:Binding Sites; D002110:Caffeine / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D005260:Female; D007537:Isometric Contraction; D017275:Isradipine / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000187:drug effects / Q000293:injuries*; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D013643:Tarsus, Animal; D019415:Torque; D014947:Wounds and Injuries / Q000378:metabolism / Q000503:physiopathology
chemical_list: D020746:Calcium Channels, L-Type; D019837:Ryanodine Receptor Calcium Release Channel; D002110:Caffeine; D017275:Isradipine