title: Formalization of the MESF unit of fluorescence intensity.
abstract: This report summarizes the work performed during the past two years at the National Institute of Standards and Technology (NIST) in the refinement and formal definition of the MESF unit of fluorescence intensity. In addition to the theory underlying the MESF unit, considerations of error analysis are also presented. The details of this work may be found in the three publications of the NIST Journal of Research (www.nist.gov) listed as the references 2-4. The use of the fluorescence intensity unit provides a tool to compare quantitative fluorescence intensity measurements over time and across platforms.
authors.affiliation: Center for Quantitative Cytometry, San Juan, Puerto Rico. abe@quantcyte.org
authors.forename: Abe
authors.initials: A
authors.lastname: Schwartz
authors.forename: Adolfas K
authors.initials: AK
authors.lastname: Gaigalas
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Gerald E
authors.initials: GE
authors.lastname: Marti
authors.forename: Robert F
authors.initials: RF
authors.lastname: Vogt
authors.forename: E
authors.initials: E
authors.lastname: Fernandez-Repollet
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000592:standards
chemical_list: D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D005456:Fluorescent Dyes