Microanalysis of nucleic acids using the limulus G test.
The limulus G test has been used as a quantitative analysis of (1-->3)-beta-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1-->3)-beta-D-glucans or/and polynucleotides on the pico gram/ml scale.
Anada
Takahisa
T
Department of Chemical Process and Environments, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
Koumoto
Kazuya
K
Mizu
Masami
M
Karinaga
Ryouji
R
Sakurai
Kazuo
K
Shinkai
Seiji
S
D005936:Glucans / Q000032:analysis; D008033:Limulus Test / Q000379:methods* / Q000592:standards; D008832:Microchemistry / Q000379:methods; D009696:Nucleic Acids / Q000032:analysis*; D011066:Poly C / Q000032:analysis / Q000378:metabolism; D011119:Polynucleotides / Q000032:analysis / Q000378:metabolism; D012566:Sizofiran / Q000032:analysis / Q000378:metabolism; D047071:beta-Glucans
D005936:Glucans; D009696:Nucleic Acids; D011119:Polynucleotides; D047071:beta-Glucans; D011066:Poly C; D012566:Sizofiran; C033363:beta-1,3-glucan