title: Development of improved vaccines for heartwater.
abstract: Heartwater is controlled by frequent application of acaricides, which is costly, creates endemic instability and has the potential of contaminating the environment. The live blood vaccine currently available has limitations because it is laborious and inconvenient to use, difficult to standardise and can transmit other blood-borne pathogens. The UF/USAID/SADC Heartwater Research Project has conducted research on the development of two types of vaccine for heartwater. The first-generation inactivated vaccine has been intensively tested in the laboratory and subsequently field tested in four southern African countries. It protects cattle, sheep and goats against mortality from heartwater challenge. It can be modified to incorporate any Ehrlichia ruminantium strain to provide protection from field challenge. The second-generation DNA vaccine containing genes encoding immunogenic E. ruminantium proteins has been developed and evaluated in the mouse model as well as in cattle and sheep. The use of improved vaccines against heartwater would have a positive impact on livestock farming in sub-Saharan Africa and the Caribbean and could be used to control the spread of heartwater if it were to be introduced into regions such as the United States.
authors.affiliation: University of Florida/USAID/SADC Heartwater Research Project, Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-0880, USA. sumanmah@mweb.co.za
authors.forename: S M
authors.initials: SM
authors.lastname: Mahan
authors.forename: A F
authors.initials: AF
authors.lastname: Barbet
authors.forename: M J
authors.initials: MJ
authors.lastname: Burridge
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D016995:Ehrlichia ruminantium / Q000235:genetics / Q000276:immunology; D006357:Heartwater Disease / Q000276:immunology* / Q000517:prevention & control; D017282:Tick-Borne Diseases / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D013987:Ticks / Q000382:microbiology; D019444:Vaccines, DNA / Q000627:therapeutic use*
chemical_list: D001428:Bacterial Vaccines; D019444:Vaccines, DNA