[Effect of modification of cell calcium status on lectin activity].
Effects of oryzalin (10 microM), an inhibitor of microtubule polymerization, on the activity of soluble and cell wall lectins were studied in 7 day-old seedlings of unhardened (23 degrees C) and cold acclimated (7 days at 2-3 degrees C) winter wheat (Triticum aestivum L.). Seedlings were grown in the presence of 25 microM and 1 mM Ca2+, 500 microM verapamil, 250 microM chlorpromazine or without modifiers of calcium status in the medium. Inhibitor of the microtubule polymerization inhibitor, likely as inhibitors of Ca(2+)-signal, decreased the activity of soluble lectins and increased that of cell wall lectins. Apparently, injury of microtubule phosphorylation results in a more considerable microtubule disorganization, than that observed after oryzalin effect. A low Ca2+ concentration (25 microM) depressed, while a high concentration (1 mM) prompted microtubule sensibility to oryzalin. Such an effect of high Ca2+ concentration may be related to destabilizative action of Ca(2+)-calmodulin in these conditions, because chlorpromazine decreased oryzalin-induced increase in the activity of cell wall lectins with 1 mM Ca2+. It is concluded that the activity of cell wall lectins depends on the microtubule status that is regulated by calcium signal.
Beliaeva
N E
NE
Department of Plant Physiology and Biotechnology of Kazan' State University.
Garaeva
L D
LD
Timofeeva
O A
OA
Chulkova
Iu Iu
IuIu
Khokhlova
L P
LP
D002118:Calcium / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002746:Chlorpromazine / Q000494:pharmacology; D003080:Cold Temperature; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009574:Nitro Compounds / Q000494:pharmacology; D037121:Plant Lectins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014908:Triticum / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
C478646:3,5-dinitro-N(4),N(4)-dipropylsulfanyl amide; D009574:Nitro Compounds; D037121:Plant Lectins; D002118:Calcium; D002746:Chlorpromazine