title: Determination of new derivatives of genistein in culture media by liquid chromatography.
abstract: Methods for determination of genistein and its four new analogues in culture media have been developed to support studies on their potential anticancer activities. The investigated compounds were extracted from the media using liquid-liquid extraction with appropriate solvent. After evaporation of organic solvents each of the dry extracts was reconstituted in appropriate mobile phase. Reversed-phase HPLC was applied to quantitative determining of tested compounds. The methods are specific, sensitive and technically simple. They were used to evaluate concentration level of investigated compounds in experiments with human promyelocytic leukemia cells (HL-60 cell line).
authors.affiliation: Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793, Warsaw, Poland. hanka@ifarm.waw.pl
authors.forename: Hanna
authors.initials: H
authors.lastname: Ksycińska
authors.forename: Barbara
authors.initials: B
authors.lastname: Sobik
authors.forename: Joanna
authors.initials: J
authors.lastname: Popiołkiewicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Polkowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Krzeczyński
authors.forename: Jan
authors.initials: J
authors.lastname: Ramza
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Pucko
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Grynkiewicz
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D019833:Genistein / Q000032:analysis*; D018922:HL-60 Cells; D006801:Humans; D012680:Sensitivity and Specificity; D012997:Solvents
chemical_list: D003470:Culture Media; D012997:Solvents; D019833:Genistein