title: Multiharmonic-generation biopsy of skin.
abstract: Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.
authors.affiliation: Department of Electrical Engineering and Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei 10617, Taiwan. sun@cc.ee.ntu.edu.tw
authors.forename: Chi-Kuang
authors.initials: CK
authors.lastname: Sun
authors.forename: Cheng-Chi
authors.initials: CC
authors.lastname: Chen
authors.forename: Shi-Wei
authors.initials: SW
authors.lastname: Chu
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Tsai
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Chen
authors.forename: Bai-Ling
authors.initials: BL
authors.lastname: Lin
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods*; D020405:Dermis / Q000473:pathology; D004423:Ear / Q000473:pathology; D007834:Lasers; D051379:Mice; D017785:Photons; D012867:Skin / Q000473:pathology*