title: Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors.
abstract: Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.
authors.affiliation: Dipartimento di Chimica, Universita' degli Studi di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, Firenze, Italy. fausto.lucarelli@unifi.it
authors.forename: Fausto
authors.initials: F
authors.lastname: Lucarelli
authors.forename: Giovanna
authors.initials: G
authors.lastname: Marrazza
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
authors.forename: Marco
authors.initials: M
authors.lastname: Mascini
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000138:chemical synthesis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D006046:Gold; D006801:Humans; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D014159:Transducers
chemical_list: D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D002244:Carbon; D006046:Gold; D004247:DNA