title: Activities of Pt(II) and Ru(III) triazole-pyrimidine complexes against Trypanosoma cruzi and T. brucei brucei.
abstract: We studied the biological activity of three newly synthesized metal complexes of triazole-pyrimidine derivatives that were previously observed to inhibit in vitro growth of epimastigotes of Trypanosoma cruzi and procyclic forms of Trypanosoma brucei brucei. We analyzed the possible inhibitory effect of these compounds on the synthesis of DNA, RNA and protein, ultrastructure and excretion of metabolites by these parasites. RNA synthesis was inhibited by all three complexes assayed. These complexes also led to anomalies of the main organelles (e.g. nucleus, kinetoplast and mitochondria). In addition, these complexes may be capable of altering the excretion of metabolites by the parasites.
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Magán
authors.forename: Clotilde
authors.initials: C
authors.lastname: Marín
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Rosales
authors.forename: Mario Antonio
authors.initials: MA
authors.lastname: Barrera
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Salas
authors.forename: Manuel
authors.initials: M
authors.lastname: Sánchez-Moreno
mesh_terms: D000818:Animals; D001711:Biotransformation; D016054:DNA, Protozoan / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000378:metabolism / Q000494:pharmacology*; D009944:Organoplatinum Compounds / Q000378:metabolism / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000096:biosynthesis; D011743:Pyrimidines / Q000378:metabolism / Q000494:pharmacology*; D016053:RNA, Protozoan / Q000096:biosynthesis; D012428:Ruthenium / Q000378:metabolism / Q000494:pharmacology*; D014230:Triazoles / Q000378:metabolism / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014529:Uridine / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D009942:Organometallic Compounds; D009944:Organoplatinum Compounds; D015800:Protozoan Proteins; D011743:Pyrimidines; D016053:RNA, Protozoan; D014230:Triazoles; D012428:Ruthenium; D014529:Uridine