Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates.
Analysis methods are described which integrate information from fluctuation analysis with that from deconvolution. Together the two approaches allow to derive a consistent quantitative description of quantal release (both evoked, spontaneous and asynchronous) under conditions in which quantal parameters may change during a repetitively applied stimulation protocol. Specifically, our methods take into account the effects of accumulating transmitter in the synaptic cleft and postsynaptic receptor desensitization, which may develop during strong stimulation. Several ways to handle non-stationarities are described. Examples are provided for the Calyx of Held, a glutamatergic synapse, in which both the pre- and the postsynaptic compartments can be voltage-clamped.
Neher
Erwin
E
Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. eneher@gwdg.de
Sakaba
Takeshi
T
D000465:Algorithms; D016477:Artifacts; D001306:Auditory Pathways / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism; D008959:Models, Neurological; D015233:Models, Statistical; D018377:Neurotransmitter Agents / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000502:physiology; D013570:Synaptic Membranes / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism*
D018377:Neurotransmitter Agents; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid