In vivo comparison of IVDU and IVFRU in HSV1-TK gene expressing tumor bearing rats.
(E)-5-(2-Iodovinyl)-2'-deoxyuridine (IVDU) and (E)-5-(2-iodovinyl)-2'-fluoro-2'-deoxyuridine (IVFRU) are potential substrates of Herpes Simplex Virus type 1 thymidine kinase (HSV-TK). In the present study, cellular uptake of radioiodinated substrates was found to be low in wild type MCA cells, but high in HSV-TK gene expressing cells. The carrier-free substrates, in particular, showed higher cellular uptake than carrier-added compounds. Biodistribution showed that the %ID/g of the MCA-TK/MCA tumor ratio of IVDU injected at 1, 4, and 24h were 1.1, 0.9 and 1.3, and those of IVFRU were 1.7, 1.7 and 1.8 respectively. Therefore, both IVDU and IVFRU could possibly be used as radiopharmaceuticals to evaluate reporter gene expression. However, IVFRU was more specific and stable than IVDU for selective non-invasive imaging of HSV-TK expression.
Choi
Tae Hyun
TH
Laboratory of Cyclotron Application, Korea Cancer Center Hospital, and Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Seoul, South Korea.
Ahn
Soon Hyuk
SH
Kwon
Hee-Chung
HC
Choi
Chang Woon
CW
Awh
Ok Doo
OD
Lim
Sang Moo
SM
D000818:Animals; D045744:Cell Line, Tumor; D016903:Drug Monitoring / Q000379:methods*; D004355:Drug Stability; D005467:Floxuridine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D015316:Genetic Therapy / Q000379:methods; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics; D007065:Idoxuridine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D009369:Neoplasms / Q000628:therapy*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D013379:Substrate Specificity; D013937:Thymidine Kinase / Q000032:analysis* / Q000235:genetics; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D005467:Floxuridine; C060868:5-(2-iodovinyl)-1-(2'-fluoro-2'-deoxyuridine); C030360:5-(2-iodovinyl)-2'-deoxyuridine; D013937:Thymidine Kinase; D007065:Idoxuridine