title: Single-level dynamic spiral CT of hepatocellular carcinoma: correlation between imaging features and density of tumor microvessels.
abstract: OBJECTIVE
  To investigate the correlation of enhancement features of hepatocellular carcinoma (HCC) revealed by single-level dynamic spiral CT scanning (DSCT) with tumor microvessel density (MVD), and to determine the validity of DSCT in assessing in vivo tumor angiogenic activity of HCC.
  
  
  METHODS
  Twenty six HCC patients were diagnosed histopathologically. DSCT was performed for all patients according to standard scanning protocol. Time-density curves were generated, relevant curve parameters were measured, and gross enhancement morphology was analyzed. Operation was performed to remove HCC lesions 1 to 2 weeks following CT scan. Histopathological slides were carefully prepared for the standard F(8)RA immunohistochemical staining and tumor microvessel counting. Enhancement imaging features of HCC lesions were correlatively studied with tumor MVD and its intra-tumor distribution characteristics.
  
  
  RESULTS
  On DSCT images of HCC lesions, three patterns of time-density curve and three types of gross enhancement morphology were recognized. Histomorphologically, the distribution of positively stained tumor endothelial cells within tumor was categorized into 3 types. Curve parameters such as peak enhancement value and contrast enhancement ratio were significantly correlated with tumor tissue MVD (r=0.508 and r=0.423, P<0.01 and P<0.05 respectively). Both the pattern of time-density curve and the gross enhancement morphology of HCC lesions were also correlated with tumor MVD, and reflected the distributive features of tumor microvessels within HCC lesions. Correlation between the likelihood of intrahepatic metastasis of HCC lesions with densely enhanced pseudocapsules and rich pseudocapsular tumor MVD was found.
  
  
  CONCLUSIONS
  Enhancement imaging features of HCC lesions on DSCT scanning are correlated with tumor MVD, and reflect the intra-tumor distribution characteristics of tumor microvessels. DSCT is valuable in assessing the angiogenic activity and tumor neovascularity of HCC patients in vivo.
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. wxchen25@sohu.com
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Chen
authors.forename: Peng-Qiu
authors.initials: PQ
authors.lastname: Min
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.forename: Bong-Liang
authors.initials: BL
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Ge
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000473:pathology; D004730:Endothelium, Vascular / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000473:pathology; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000473:pathology; D036542:Tomography, Spiral Computed; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D014841:von Willebrand Factor