title: Action potential modulation of connexin40 gap junctional conductance.
abstract: Connexin40 (Cx40) is abundantly expressed in the atrial myocardium, ventricular conduction system, and vascular endothelial and smooth muscle cells of the mammalian cardiovascular system. Rapid conduction through cardiac tissues depends on electrotonic transfer of the action potential between neighboring cells. To determine whether transjunctional voltages (Vj) elicited by an action potential can modulate conductance of Cx40 gap junctions, simulated myocardial action potentials were applied as voltage-clamp waveforms to Cx40 gap junctions expressed in mouse neuro2A (N2A) cells. Junctional currents resembled the action potential morphology but declined by >50% from peak to near-constant plateau values. Kinetics of Cx40 voltage gating were examined at peak voltages > or =100 mV, and decay time constants changed e-fold per 17.6 mV for Vj > +/-40 mV. Junctional conductance recovered during phase 3 repolarization and early diastole to initial values. These phasic changes in junctional conductance were due to rapid decay kinetics, increasing to tens of milliseconds at peak Vj of 130 mV, and the increase in the steady-state conductance curve as Vj returned toward 0 mV. Time-dependent conductance curves for Cx40 were modeled with one inactivation and two recovery Vj-dependent components. There was a temporal correlation between development of conduction delay or block and the inactivation phase of junctional conductance. Likewise, recovery of junctional conductance was coincident with recovery from refractoriness, suggesting that gap junctions may play a role in the genesis and propagation of cardiac arrhythmias.
authors.affiliation: Department of Pharmacology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA.
authors.forename: Xianming
authors.initials: X
authors.lastname: Lin
authors.forename: Richard D
authors.initials: RD
authors.lastname: Veenstra
mesh_terms: D000200:Action Potentials; D000818:Animals; D045744:Cell Line, Tumor; D017630:Connexins / Q000502:physiology*; D004553:Electric Conductivity; D017629:Gap Junctions / Q000502:physiology*; D051379:Mice; D008954:Models, Biological; D051381:Rats; D013997:Time Factors; D000097003:Gap Junction alpha-5 Protein
chemical_list: D017630:Connexins; D000097003:Gap Junction alpha-5 Protein