[Effects of tobacco on proliferation and attachment of human periodontal ligament fibroblast].
OBJECTIVE
To evaluate the effects of nicotine and tobacco extract (ST) on PDLFs morphology, structure, proliferation and attachment.


METHODS
PDLFs were cultured in the presence of nicotine and ST at various concentration. The cell changes in the morphology and structure were examined by histological and transmission electrical microscope (TEM). The growth and attachment of cell were measured by MTT method.


RESULTS
The size of the cells became smaller gradually and their shapes changed from shuttle type to oval or round when the concentration of nicotine and ST increased, the polarity of the cells was in disorder, ultrastructure showed that the organelles, especially rough-surfaced endoplasmic reticulum and golgi complex decreased in number, microtubule and microfilaments were disassembled, the nuclei became fewer or shrunk, the growth and attachment were dose-dependently inhibited.


CONCLUSIONS
Nicotine and ST can change PDLFs' morphology and structure, they may inhibit the growth and attachment through disruption of the cytoskeleton, suggesting nicotine and ST may have pathological role on human periodontitis.
Xu
Yan
Y
Department of Oral Medicine, School of Stomatology, Anhui Medical University, Hefei 230032, China.
Jiang
Yong
Y
Li
Song
S
Mei
Ling-xXuan
LX
Zhang
Li
L
Zhao
Chun-hHui
CH
D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D009538:Nicotine / Q000633:toxicity; D010513:Periodontal Ligament / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D010936:Plant Extracts / Q000633:toxicity; D014026:Nicotiana
D010936:Plant Extracts; D009538:Nicotine