Effects of dopamine infusion on cerebral blood flow, brain cell membrane function and energy metabolism in experimental Escherichia coli meningitis in the newborn piglet.
In the present study, we tested whether maintenance of adequate cerebral perfusion pressure (CPP) by pharmacologically preventing systemic hypotension with dopamine infusion would prevent cerebral ischemia and attenuate energy depletion and neuronal injury even though intracranial pressure remains elevated in a newborn piglet meningitis model. Cerebral blood flow, measured at the end of the experiment using fluorescent microspheres, was significantly increased by dopamine infusion. The decreased cerebral cortical cell membrane Na+, K+ -ATPase activity and increased lipid peroxidation products, indicative of meningitis-induced brain damage, were significantly attenuated by dopamine infusion. Dopamine also significantly attenuated the meningitis-induced reduction in both brain ATP and phosphocreatine levels and the increase in brain lactate level. In summary, maintenance of adequate CPP with dopamine prevented cerebral ischemia, reduced cerebral energy depletion, and attenuated brain injury in neonatal bacterial meningitis.
Park
Won Soon
WS
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. wspark@smc.samsung.co.kr
Chang
Yun Sil
YS
Shim
Jae Won
JW
Kim
Mi Jung
MJ
Ko
Sun Young
SY
Kim
Sung Shin
SS
Hwang
Jong Hee
JH
Choi
Chang Won
CW
Lee
Munhyang
M
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000378:metabolism; D006439:Hemodynamics; D020814:Meningitis, Escherichia coli / Q000378:metabolism*; D008863:Microspheres; D011897:Random Allocation; D012039:Regional Blood Flow; D013552:Swine
D005456:Fluorescent Dyes; D004298:Dopamine