Characterization and localization of fluorescent Pseudomonas cold shock protein(s) by monospecific polyclonal antibodies.
Cold shock protein (CSP) from Pseudomonas fluorescens MTCC 103 and cold resistant protein (CRP) from its mutant CRPF8 of 14 and 35 kd, respectively were purified to homogeneity by HPLC. Polyclonal antibodies were raised against these proteins and the expression level was checked at different temperatures, i.e., 4, 10, 20, 30 and 37 C. Furthermore, morphological changes in P. fluorescens MTCC 103 and its mutant (CRPF8) were analyzed by transmission electron microscopy (TEM). Localization of CSP and CRP documented with immunoelectron microscopy, using colloidal gold particles conjugated with secondary antibodies being the probe were used. Nevertheless, the results of cytosolic localization of CSP and CRP were evident. Furthermore, the expression of CSP and CRP increased with decrease in temperature and the cell wall thickness of the mutant exhibited 2-fold increase, thus facilitating low temperature survival.
Khan
Mahejibin
M
Department of Microbiology, College of Basic Sciences & Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar-263145 (US Nagar), Uttaranchal, India.
Bajpai
Virendra Kumar
VK
Anasari
Shaheen Akhtar
SA
Kumar
Anil
A
Goel
Reeta
R
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D002473:Cell Wall / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D015151:Immunoblotting; D008854:Microscopy, Electron; D008970:Molecular Weight; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature
D001426:Bacterial Proteins