title: Predominant intracellular expression of CXCR4 and CCR5 in purified primary trophoblast cells from first trimester and term human placentae.
abstract: OBJECTIVE
  The aim of the present study was to define the expression of CXCR4 and CCR5 on non-cultured non-stimulated primary human trophoblast cells (TCs) immediately after their immunopurification.
  
  
  METHODS
  We have evaluated by flow cytometric analysis and immunofluorescence, highly purified primary TCs prepared from first trimester (8.2 +/- 0.3 weeks, n = 15) and term (Caesarean section, n = 10) placentae for the cell surface and intracellular expression of CXCR4 and CCR5.
  
  
  RESULTS
  There was a high level of individual variability for CXCR4 and CCR5 expression between trophoblast batches. In first trimester and term placentae TCs, we found a greater number of TCs preparations expressing intracellular CXCR4 than CCR5 (P < 0.05). Both receptors were predominantly localized in the intracellular compartment of TCs, whatever if isolated from first trimester or term placentae.
  
  
  CONCLUSIONS
  The functional consequences of the predominance of CXCR4 expression and of cellular addressing are briefly discussed.
authors.affiliation: INSERM U 131, Equipe Cytokines et Relation Materno-Foetale, Hôpital Béclère, Clamart, France. juanguimal@epm.net.co
authors.forename: Juan
authors.initials: J
authors.lastname: Maldonado-Estrada
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Menu
authors.forename: Pierre
authors.initials: P
authors.lastname: Roques
authors.forename: Bruno
authors.initials: B
authors.lastname: Vaslin
authors.forename: Alice
authors.initials: A
authors.lastname: Dautry-Varsat
authors.forename: Françoise
authors.initials: F
authors.lastname: Barré-Sinoussi
authors.forename: Gérard
authors.initials: G
authors.lastname: Chaouat
mesh_terms: D002451:Cell Compartmentation; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000276:immunology; D008856:Microscopy, Fluorescence; D010920:Placenta / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D019713:Receptors, CCR5 / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology*
chemical_list: D019713:Receptors, CCR5; D019718:Receptors, CXCR4