[Time-effect and dose-effect relationships study on the effect of benefiting-bone capsule on osteoblasts proliferation].
OBJECTIVE
To investigate the effect of rats sera containing Benefiting-bone Capsule (BBC) on the cell proliferation of skull osteoblast in neonatal SD rats.


METHODS
Forty female SD rats, aged 12 months, were randomly divided into 4 groups including N.S. group, low dosage, middle dosage and high dosage BBC group, the sera were obtained at different times. MTT method was used to determine the effect of BBC on osteoblasts proliferation.


RESULTS
The MTT value reached its highest point 1.5 hours after oral administration (P < 0.05). MTT of high dosage BBC met the maximum value with the concentration of 25% (P < 0.05).


CONCLUSIONS
The rats sera containing BBC can promote proliferation of osteoblast.
Shu
Xiaochun
X
Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000.
Zhang
Ronghua
R
Peng
Keping
K
Zhu
Xiaofeng
X
Cai
Yu
Y
D000818:Animals; D002214:Capsules; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D002214:Capsules; D004365:Drugs, Chinese Herbal