title: Application of LC-MS to the analysis of new radiopharmaceuticals.
abstract: Liquid chromatography in combination with mass spectrometry (LC-MS) is key to the identification of metabolites in new molecular imaging probes. These metabolites can be generated using hepatocytes from various species in vitro. Metabolites produced from 4-fluoro-N-[2-[4-(2-methoxylphenyl)piperazino]ethyl]-N-(2-pyridyl)cyclohexane carboxamide (FCWAY) using rat and human hepatocytes are different, whereas metabolites from (3-(3-fluoropropylthio)-1,2,5-thiadiazol-4-yl)-tetrahydro-l-methyl-pyridine (FP-TZTP) using rat and human hepatocytes were similar. The combination of LC-MS and hepatocytes are an indispensable tool in the development of new molecular imaging probes.
authors.affiliation: PET Department, Warren G. Magnuson Clinical Center, NIH, Bethesda, MD 20892, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Dale
authors.initials: D
authors.lastname: Kiesewetter
authors.forename: Lixin
authors.initials: L
authors.lastname: Lang
authors.forename: William C
authors.initials: WC
authors.lastname: Eckelman
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005462:Fluorine Radioisotopes; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010879:Piperazines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011725:Pyridines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019275:Radiopharmaceuticals / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013844:Thiazoles / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: C114151:4-fluoro-N-(2-(1-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-2-pyridinylbenzamide; D005462:Fluorine Radioisotopes; D010879:Piperazines; D011725:Pyridines; D019275:Radiopharmaceuticals; D013844:Thiazoles; C115177:FP-TZTP