Collagen-induced STAT family members activation in Entamoeba histolytica trophozoites.
The interaction of Entamoeba histolytica trophozoites with collagen type I and calcium induces a membrane to nuclei signaling. The transduction pathways involved in such phenomena are still poorly understood. Using a combination of immunoprecipitation assays, Western immunoblot analysis, electrophoretic mobility shift assays and immunocytochemistry we demonstrate here the expression, tyrosine phosphorylation, nuclear translocation and DNA binding of two members of the signal transducers and activators of transcription family of inducible transcription factors in the protozoan parasite E. histolytica. These results support the notion that the interaction of the extracellular matrix components with the parasite turns on a genetic program that facilitates the invasion of the host.
Cruz-Vera
Jorge
J
Departamento de Genética y Biología Molecular, Cinvestav-IPN, Apartado Postal 14-740, México DF, 07300 México, Mexico.
Clara
L
L
Hernández-Kelly
R
R
Alfredo Méndez
J
J
Pérez-Salazar
Eduardo
E
Ortega
Arturo
A
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D024042:Collagen Type I / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D004748:Entamoeba histolytica / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D024042:Collagen Type I; D004268:DNA-Binding Proteins; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D015534:Trans-Activators; D014443:Tyrosine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium