title: A consensus linkage map for sugi (Cryptomeria japonica) from two pedigrees, based on microsatellites and expressed sequence tags.
abstract: A consensus map for sugi (Cryptomeria japonica) was constructed by integrating linkage data from two unrelated third-generation pedigrees, one derived from a full-sib cross and the other by self-pollination of F1 individuals. The progeny segregation data of the first pedigree were derived from cleaved amplified polymorphic sequences, microsatellites, restriction fragment length polymorphisms, and single nucleotide polymorphisms. The data of the second pedigree were derived from cleaved amplified polymorphic sequences, isozyme markers, morphological traits, random amplified polymorphic DNA markers, and restriction fragment length polymorphisms. Linkage analyses were done for the first pedigree with JoinMap 3.0, using its parameter set for progeny derived by cross-pollination, and for the second pedigree with the parameter set for progeny derived from selfing of F1 individuals. The 11 chromosomes of C. japonica are represented in the consensus map. A total of 438 markers were assigned to 11 large linkage groups, 1 small linkage group, and 1 nonintegrated linkage group from the second pedigree; their total length was 1372.2 cM. On average, the consensus map showed 1 marker every 3.0 cM. PCR-based codominant DNA markers such as cleaved amplified polymorphic sequences and microsatellite markers were distributed in all linkage groups and occupied about half of mapped loci. These markers are very useful for integration of different linkage maps, QTL mapping, and comparative mapping for evolutional study, especially for species with a large genome size such as conifers.
authors.affiliation: Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Tani
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Iwata
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mukai
authors.forename: Tokuko
authors.initials: T
authors.lastname: Ujino-Ihara
authors.forename: Asako
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Kensuke
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshimaru
authors.forename: Masafumi
authors.initials: M
authors.lastname: Murai
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Nagasaka
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tsumura
mesh_terms: D001483:Base Sequence; D032485:Cryptomeria / Q000235:genetics*; D017931:DNA Primers; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree
chemical_list: D017931:DNA Primers; D005819:Genetic Markers