In vitro and ex vivo blood compatibility study of 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymer-coated hemodialysis hollow fibers.
To identify the advantages of 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymer-coated polysulfone (PSf) hollow fibers for hemodialyzer and hemofilter minimodules with hollow fibers were made and blood compatibility was evaluated in vitro and ex vivo. Three types of hollow fibers, i.e., pure PSf (no additives), PSf alloyed with poly(1-vinyl-2-pyrrolidone) (PVPy), and PSf coated with the MPC copolymer, were processed in wet conditions. Commercially available hollow fibers (APS) were used as a control sample. The PSf hollow fibers have a condensed structure. A porous structure was observed when the PVPy was alloyed before wet processing, and no effect of the innercoated MPC copolymer on the porous structure was observed. One-tenth-sized minimodules of the conventional hemodialyzer were fabricated with 200 fibers each. The solute permeability of the hollow fibers was evaluated using 10% bovine serum in a buffer solution containing cytochrome C, which is a model protein of Beta(2)-microglobulin. After circulation for 2.5 h, the solute permeability of APS and PVPy-alloyed PSf hollow fibers decreased to 50% compared with their initial values. In contrast, the value for the hollow fibers innercoated with the MPC copolymer maintained its initial level. The inner surface of the dialysis membranes was observed with a transmission electron microscope and a layer of adsorbed protein on the PSf, APS, and PVPy-alloyed PSf hollow fibers was observed, but not on the MPC copolymer-coated fibers. Blood cell adhesion was then evaluated by circulation of whole rabbit blood without any anticoagulant ex vivo. Many adherent cells were observed on the PVPy-alloyed PSf hollow fibers; however, blood cells did not adhere or aggregate on the MPC copolymer-coated hollow fibers. From these results, we concluded that the in-situ coating of MPC copolymer on PSf hollow fibers is effective in preventing blood coagulation and maintaining the solute permeability of the fibers.
Iwasaki
Yasuhiko
Y
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan. yasu.org@tmd.ac.jp
Nakabayashi
Nobuo
N
Ishihara
Kazuhiko
K
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000628:therapy; D008422:Materials Testing; D008567:Membranes, Artificial; D010767:Phosphorylcholine / Q000737:chemistry / Q000494:pharmacology*; D010973:Platelet Adhesiveness; D011102:Polyhydroxyethyl Methacrylate / Q000494:pharmacology*; D011817:Rabbits; D006435:Renal Dialysis / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D010767:Phosphorylcholine; D011102:Polyhydroxyethyl Methacrylate