Isolation of pathogenic Listeria monocytogenes and detection of antibodies against phosphatidylinositol-specific phospholipase C in buffaloes.
The isolation of pathogenic Listeria spp. in bacteriological samples, and anti-phosphatidylinositol-specific phospholipase C (anti-PIPLC) antibodies in sera of buffaloes were studied. Isolation of the pathogen was attempted from the samples by selective enrichment in University of Vermont Medium and plating onto Dominguez-Rodriguez isolation agar. Pathogenicity of the isolates was tested by Christie, Atkins, Munch Petersen test and mice incoulation test. Listeria spp. and L. monocytogenes were isolated from 8.8 and 2.4%, and 4.8 and 1.6% of 125 each meat and blood samples, respectively. Out of the 125 samples each of feacal, nasal and vaginal swabs from buffaloes 8 and 4%, 13.6 and 2.4%, and 6.4 and 2.4% were positive for Listeria spp. and L. monocytogenes, respectively. L. ivanovii was confirmed from 0.8% vaginal sample. A total of 125 serum samples were tested by phosphatidylinositol-specific phospholipase C (PIPLC) based indirect ELISA of which 4.0% turned out to be seropositive.
Chaudhari
S P
SP
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Malik
S V S
SV
Chatlod
L R
LR
Barbuddhe
S B
SB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001681:Biological Assay / Q000662:veterinary; D002020:Buffaloes / Q000276:immunology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D008089:Listeria monocytogenes / Q000201:enzymology / Q000302:isolation & purification*; D008088:Listeriosis / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D008460:Meat / Q000382:microbiology; D051379:Mice; D009296:Nasal Cavity / Q000382:microbiology; D043265:Phosphatidylinositol Diacylglycerol-Lyase / Q000276:immunology*; D054801:Phosphoinositide Phospholipase C
D000907:Antibodies, Bacterial; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase