title: In vivo visualization of metastatic prostate cancer and quantitation of disease progression in immunocompromised mice.
abstract: While survival periods for patients with localized prostate cancer have increased, there is still no curative therapy for metastatic disease. Using non-invasive bioluminescent imaging, we designed a comprehensive murine model to monitor tumor location and expansion. We detected micrometastases after one week that correlated by gross necropsy, autoradiography, and histopathology with organ and skeletal lesions seen clinically. We calculated in vivo kinetics for tumor growth based on biophoton emissions and observed significantly faster growth of bone lesions and of overall tumor burden in young mice compared to old mice. This model provides a controllable biological system for further investigation into the pathogenesis of metastatic prostate cancer and evaluation of new therapies.
authors.affiliation: Department of Urology, Division of Hematology and Oncology, The University of Michigan Comprehensive Cancer Center, Ann Arbor, Michigan 48109-0946, USA.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Kalikin
authors.forename: Abraham
authors.initials: A
authors.lastname: Schneider
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Thakur
authors.forename: Yaron
authors.initials: Y
authors.lastname: Fridman
authors.forename: Laura B
authors.initials: LB
authors.lastname: Griffin
authors.forename: Rodney L
authors.initials: RL
authors.lastname: Dunn
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Rosol
authors.forename: Rajal B
authors.initials: RB
authors.lastname: Shah
authors.forename: Alnawaz
authors.initials: A
authors.lastname: Rehemtulla
authors.forename: Laurie K
authors.initials: LK
authors.lastname: McCauley
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Pienta
mesh_terms: D000818:Animals; D002455:Cell Division; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D017690:Cell Transplantation; D003952:Diagnostic Imaging; D018450:Disease Progression; D006801:Humans; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D010010:Osteoclasts / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D012190:Retroviridae / Q000235:genetics; D013131:Spine / Q000473:pathology / Q000503:physiopathology
chemical_list: D008156:Luciferases