title: Epoxide hydrolase Tyr113His polymorphism is not associated with susceptibility to esophageal squamous cell carcinoma in population of North China.
abstract: OBJECTIVE
  To investigate the possible association of microsomal epoxide hydrolase (mEH) Tyr113His polymorphism with susceptibility to esophageal squamous cell carcinoma (ESCC) in a population of North China.
  
  
  METHODS
  The mEH Tyr113His genotypes were determined by polymerase-chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis in 257 patients with esophageal squamous cell carcinoma (ESCC) and 252 healthy subjects as a control group.
  
  
  RESULTS
  The frequencies for Tyr and His alleles were 44.2%, 55.8% in ESCC patients, and 44.0% and 56.0% in healthy subjects, respectively. No statistic difference in allele distribution was observed between ESCC patients and controls (chi2=0.008, P=0.929). The overall genotype distribution difference was not observed between cancer cases and controls (chi2=2.116, P=0.347). Compared with Tyr/Tyr genotype, neither His/His genotype nor in combination with Tyr/His genotype significantly modified the risk of the development of ESCC, the adjusted odds ratio was 1.076 (95% CI=0.850-1.361) and 0.756 (95% CI=0.493-1.157), respectively. When stratified for sex, age, smoking status and family history of upper gastrointestinal cancer, His/His genotype alone or in combination with Tyr/His genotype also did not show any significant influence on the risk of developing ESCC.
  
  
  CONCLUSIONS
  MEH Tyr113His polymorphism may not be used as a stratification marker in screening individuals at a high risk of ESCC.
authors.affiliation: Hebei Cancer Institute, Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Jin
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Deng-Gui
authors.initials: DG
authors.lastname: Wen
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Kuang
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Wei
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002681:China; D017931:DNA Primers; D004851:Epoxide Hydrolases / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006639:Histidine; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values; D012907:Smoking; D014443:Tyrosine
chemical_list: D017931:DNA Primers; D014443:Tyrosine; D006639:Histidine; D004851:Epoxide Hydrolases