title: Effect of the Suppressor of Underreplication (SuUR) gene on position-effect variegation silencing in Drosophila melanogaster.
abstract: It has been previously shown that the SuUR gene encodes a protein located in intercalary and pericentromeric heterochromatin in Drosophila melanogaster polytene chromosomes. The SuUR mutation suppresses the formation of ectopic contacts and DNA underreplication in polytene chromosomes; SuUR+ in extra doses enhances the expression of these characters. This study demonstrates that heterochromatin-dependent PEV silencing is also influenced by SuUR. The SuUR protein localizes to chromosome regions compacted as a result of PEV; the SuUR mutation suppresses DNA underreplication arising in regions of polytene chromosomes undergoing PEV. The SuUR mutation also suppresses variegation of both adult morphological characters and chromatin compaction observed in rearranged chromosomes. In contrast, SuUR+ in extra doses and its overexpression enhance variegation. Thus, SuUR affects PEV silencing in a dose-dependent manner. However, its effect is expressed weaker than that of the strong modifier Su(var)2-5.
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Belyaeva
authors.forename: L V
authors.initials: LV
authors.lastname: Boldyreva
authors.forename: E I
authors.initials: EI
authors.lastname: Volkova
authors.forename: R A
authors.initials: RA
authors.lastname: Nanayev
authors.forename: A A
authors.initials: AA
authors.lastname: Alekseyenko
authors.forename: I F
authors.initials: IF
authors.lastname: Zhimulev
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D020868:Gene Silencing; D008297:Male
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C464850:SuUR protein, Drosophila; D004247:DNA