Sensitivity of Vibrio species in phosphate-buffered saline and in oysters to high-pressure processing.
Multiple strains of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae non-O1 were tested in phosphate-buffered saline for their sensitivity to high-pressure processing (HPP). Variability in sensitivity among strains was observed for all species; this variability decreased at higher pressures. V. vulnificus was the species that was most sensitive to treatment at 200 MPa (decimal reduction time [D] = 26 s), and V. cholerae was the species that was most resistant to treatment at 200 MPa (D = 149 s). The O3:K6 serotype of V. parahaemolyticus was more resistant to pressure than other serotypes of V. parahaemolyticus were. The results of studies involving V. vulnificus naturally occurring in oysters revealed that a pressure treatment of 250 MPa for 120 s achieved a > 5-log reduction in the levels of this bacterium. V. parahaemolyticus serotype O3:K6 in oysters required a pressure of 300 MPa for 180 s for a comparable 5-log reduction. When properly applied, HPP can be effective in improving the safety of shellfish with respect to Vibrio spp.
Cook
David W
DW
U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, Alabama 36528-0158, USA. docjoc@cableone.net
D000818:Animals; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D010125:Ostreidae / Q000382:microbiology*; D011312:Pressure; D012703:Serotyping; D012758:Shellfish / Q000382:microbiology*; D013045:Species Specificity; D014733:Vibrio / Q000145:classification / Q000187:drug effects / Q000254:growth & development*; D044163:Vibrio cholerae non-O1 / Q000187:drug effects / Q000254:growth & development; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000254:growth & development; D041261:Vibrio vulnificus / Q000187:drug effects / Q000254:growth & development