title: Hepatocellular apoptosis after hepatectomy in obstructive jaundice in rats.
abstract: OBJECTIVE
  To investigate the hepatocellular apoptosis after hepatectomy in obstructive jaundice and biliary decompression rats.
  
  
  METHODS
  After bile duct ligation for 7 days, rats were randomly divided into OB group in which the rats underwent 70% hepatectomy, OB-CD group in which the rats underwent hepatectomy accompanied by choledochoduodenostomy, CD-Hx group in which the rats underwent choledochoduodenostomy and then received 70% hepatectomy on the fifth day after biliary decompression. The control group (Hx group) only underwent hepatectomy.
  
  
  RESULTS
  The level of total serum bilirubin and serum enzymes was significantly lower in CD-Hx group than in OB-CD and OB groups on day 1, 3 and 5 after hepatectomy. The apoptotic index was significantly lower in CD-Hx group than in OB-CD and OB groups on day 3 and 5. The oligonucleosomal DNA fragments and Caspase-3 activity were also lower in CD-Hx group than in OB-CD and OB groups 3 days after hepatectomy, without differences between CD-Hx and Hx groups.
  
  
  CONCLUSIONS
  Hepatocellular apoptosis plays vital roles in jaundice rats, and biliary decompression is more effective in treatment of patients with severe jaundice before operation.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, Shannxi Province, China. wangdesh@163.com
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Ke-Feng
authors.initials: KF
authors.lastname: Dou
authors.forename: Kai-Zong
authors.initials: KZ
authors.lastname: Li
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Gao
authors.forename: Zhen-Shun
authors.initials: ZS
authors.lastname: Song
authors.forename: Zheng-Cai
authors.initials: ZC
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001663:Bilirubin / Q000097:blood; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002768:Choledochostomy; D004195:Disease Models, Animal; D006498:Hepatectomy; D041781:Jaundice, Obstructive / Q000097:blood / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D001663:Bilirubin