[The effect of extremely low frequency magnetic fields on cytochrome oxidase subunit 1 mRNA transcription].
OBJECTIVE
To clone and identify MF-1 gene which responded to extremely low frequency magnetic fields(ELF MF) in Daudi cells, and explore the response universality of MF-1 gene in several MF-sensitive cell lines, so as to provide experimental basis for revealing the mechanism of biological effects induced by magnetic field.


METHODS
The DNA fragment of MF-1 was cloned and sequenced; the mRNA level of MF-1 gene were analysed in MF-sensitive cell lines(HL-60, L1210 and CHL) by Northern blot after these cells being treated with 0.1 mT and 0.8 mT MF for 20 minutes and 24 hours, respectively.


RESULTS
The MF-1 cDNA sequence had 100% homology with cytochrome oxidase subunit 1 gene(CO1) by searching Gene Bank database; the transcription of CO1 in HL-60, L1210 and CHL cell lines which exposed to 0.1 mT and 0.8 mT MF for 20 minutes were significantly lower(0.38 +/- 0.12 and 0.37 +/- 0.04) than that of control(0.58 +/- 0.12) and so did for 24 hours exposure(0.46 +/- 0.09 and 0.45 +/- 0.09 vs 0.65 +/- 0.06) (P < 0.05).


CONCLUSIONS
CO1 is a MF-responsive gene. Cytochrome oxidase activity may be affected through low level of CO1 transcription by magnetic fields, thus induce bioeffects in organisms.
Zhong
Tao
T
Bioelectromagnetic Lab., Zhejiang University School of Medicine, Hangzhou 310031, China.
Chen
Qing
Q
Wu
Ruiying
R
Yao
Gengdong
G
Lu
Deqiang
D
Chiang
Huai
H
D000818:Animals; D006224:Cricetinae; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D018922:HL-60 Cells; D006801:Humans; D007939:Leukemia L1210; D008280:Magnetics; D051379:Mice; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis*; D014158:Transcription, Genetic / Q000528:radiation effects*
D021122:Protein Subunits; D012333:RNA, Messenger; D003576:Electron Transport Complex IV