title: Autologous peripheral retinal pigment epithelium translocation in patients with subfoveal neovascular membranes.
abstract: OBJECTIVE
  To evaluate the possibility of translocating autologous peripheral retinal pigment epithelial (RPE) cells and enhance their adhesion to improve functional outcome after choroidal neovascular membrane extraction in patients with subfoveal neovascular membranes.
  
  
  METHODS
  A prospective, non-controlled surgical study in eight consecutive patients operated between February and July 2001 with final data monitoring in July 2002. All patients had mixed subfoveal membranes of 2-4 disc diameters. Functional tests included Snellen vision and central fixation testing. During vitrectomy, after the extraction of the neovascular complex, 8 x 10(4)-16 x 10(4) RPE cells were removed from the periphery and translocated under the macula following the submacular injection of 2 microg of poly-L-lysine to promote adhesion of the cells.
  
  
  RESULTS
  With a follow up ranging from 3 months to 16 months, a pigmented area was seen in the extraction bed of the neovascular membrane in only one patient. Fixation was at the edge of the extraction bed in three patients. Vision remained the same in five patients and deteriorated in three (all with retinal detachment). Retinal detachment due to proliferative vitreoretinopathy occurred in three patients.
  
  
  CONCLUSIONS
  The translocation of autologous peripheral RPE cells after membrane extraction was technically possible in a sterile manner, but was associated with a high proliferative vitreoretinopathy rate and in the present series had no measurable positive effect on functional outcome.
authors.affiliation: The Rotterdam Eye Hospital, Schiedamsevest 180, 3011 BH, Rotterdam, Netherlands. janvanmeurs@cs.com
authors.forename: J C
authors.initials: JC
authors.lastname: van Meurs
authors.forename: E
authors.initials: E
authors.lastname: ter Averst
authors.forename: L J
authors.initials: LJ
authors.lastname: Hofland
authors.forename: P M
authors.initials: PM
authors.lastname: van Hagen
authors.forename: C M
authors.initials: CM
authors.lastname: Mooy
authors.forename: G S
authors.initials: GS
authors.lastname: Baarsma
authors.forename: R W
authors.initials: RW
authors.lastname: Kuijpers
authors.forename: T
authors.initials: T
authors.lastname: Boks
authors.forename: P
authors.initials: P
authors.lastname: Stalmans
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002448:Cell Adhesion; D017690:Cell Transplantation / Q000009:adverse effects / Q000379:methods; D020256:Choroidal Neovascularization / Q000209:etiology / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008268:Macular Degeneration / Q000150:complications / Q000601:surgery; D008297:Male; D010857:Pigment Epithelium of Eye / Q000473:pathology / Q000637:transplantation*; D011446:Prospective Studies; D016896:Treatment Outcome; D014792:Visual Acuity; D014821:Vitrectomy