title: Antibody-mediated neutralization of pertussis toxin-induced mitogenicity of human peripheral blood mononuclear cells.
abstract: Antibody-mediated neutralization of pertussis toxin-induced proliferation of human peripheral blood mononuclear cells (PBMC) was assessed using alamarBlue and compared with results from the Chinese hamster ovary (CHO) cell assay using sera from vaccinated adults and convalescent children. Neutralization values for the CHO assay were similar for vaccinated and convalescent subjects; however. the convalescent group had higher titers in the PBMC assay. Results for pertussis toxin neutralization with the CHO assay appear to be distinct from those with the PBMC assay.
authors.affiliation: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Scott H
authors.initials: SH
authors.lastname: Millen
authors.forename: David I
authors.initials: DI
authors.lastname: Bernstein
authors.forename: Beverly
authors.initials: B
authors.lastname: Connelly
authors.forename: Joel I
authors.initials: JI
authors.lastname: Ward
authors.forename: Swei-Ju
authors.initials: SJ
authors.lastname: Chang
authors.forename: Alison A
authors.initials: AA
authors.lastname: Weiss
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D009500:Neutralization Tests; D037342:Pertussis Toxin / Q000276:immunology* / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D037342:Pertussis Toxin