title: [Inhibition of survivin expression in liver cancer cells by shRNA].
abstract: OBJECTIVE
  To construct the plasmid containing short hairpin RNA (shRNA) of survivin in order to suppress the expression of survivin gene in HepG2 and SMMC-7721.
  
  
  METHODS
  Two 20 to 21 bp reverse repeated motifs of survivin target sequence with 4 bp or 8 bp spacer were synthesized respectively and inserted into plasmid pTZU6+1 to generate the plasmid pshRNA-survivin1 and pshRNA-survivin2; plasmid pEGFP-C1-survivin and pshRNA-survivin1 or pshRNA-survivin2 plasmid were cotransfected into liver cancer cell HepG2 and SMMC-7721 to detect effect of GFP expression respectively and analyze the inhibition of survivin gene.
  
  
  RESULTS
  The recombinant plasmid pshRNA-survivin1 and pshRNA-survivin2 were successfully constructed. The recombinant plasmids suppress the survivin expression by 80% in HepG2 and SMMC-7721.
  
  
  CONCLUSIONS
  The result showed that the short hairpin RNA of survivin can efficiently suppress it's expression in HepG2 and SMMC-7721.
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Yan
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
authors.forename: Ni
authors.initials: N
authors.lastname: Tang
authors.forename: Bing-qian
authors.initials: BQ
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Pu
authors.forename: Ming-que
authors.initials: MQ
authors.lastname: Xianh
authors.forename: Yin-hua
authors.initials: YH
authors.lastname: Lan
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008113:Liver Neoplasms / Q000235:genetics / Q000628:therapy*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D000077022:Survivin