Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.
We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice.
Cho
Eun-Min
EM
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Okada
Atsushi
A
Kenmoku
Hiromichi
H
Otomo
Kazuko
K
Toyomasu
Tomonobu
T
Mitsuhashi
Wataru
W
Sassa
Takeshi
T
Yajima
Arata
A
Yabuta
Goro
G
Mori
Kenji
K
Oikawa
Hideaki
H
Toshima
Hiroaki
H
Shibuya
Naoto
N
Nojiri
Hideaki
H
Omori
Toshio
T
Nishiyama
Makoto
M
Yamane
Hisakazu
H
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004224:Diterpenes / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology*; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012717:Sesquiterpenes; D013729:Terpenes; D014466:Ultraviolet Rays; D000094162:Phytoalexins
D018076:DNA, Complementary; D004224:Diterpenes; D009844:Oligosaccharides; D010936:Plant Extracts; D010940:Plant Proteins; D012333:RNA, Messenger; D012717:Sesquiterpenes; D013729:Terpenes; D002686:Chitin; C484246:DTC1 protein, Oryza sativa; D007535:Isomerases; D000094162:Phytoalexins