[Experimental study on apoptosis in T cells induced by ovarian carcinoma cells].
OBJECTIVE
To investigate apoptosis in T cells induced by ovarian carcinoma cells and analyze the role of nitric oxide and intracellular free calcium in this process.


METHODS
Apoptosis induced by ovarian carcinoma cell lines supernatants was investigated by electron microscopy and flow cytometry. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of inducible nitric oxide synthase (iNOS) mRNA. Calcium ions ([Ca(2+)]i) and cycle guanosine monophosphate (cGMP) were determined by Fura-2 fluorescein load technique and enzyme immunoassay (EIA), respectively.


RESULTS
Cluster of differentiation (CD)8(+) T cells cocultured with ovarian carcinoma cell lines supernatants expressed typical change of apoptosis, such as condensation and fragmentation of chromosomes, apoptosis peak in flow cytometry and higher apoptotic ratios[(23.8 +/- 3.5)%, (23.1 +/- 2.9)%, (24.2 +/- 4.9)% vs (4.6 +/- 0.5)%, P < 0.01]. The expression of iNOS mRNA and cGMP level in the process of CD(8)(+) T cellular apoptosis were significantly enhanced as compared to that of control group [1.14 +/- 0.03, 1.05 +/- 0.04, 1.16 +/- 0.02 vs 0.60 +/- 0.03, P < 0.01; (0.65 +/- 0.09), (0.62 +/- 0.16), (0.57 +/- 0.12) pmol/Lvs (0.29 +/- 0.04) pmol/ml, P < 0.01], and [Ca(2+)]i was significantly higher [(380 +/- 38), (366 +/- 13), (356 +/- 20) nmol/L vs (184 +/- 11) nmol/L, P < 0.01].


CONCLUSIONS
Nitric oxide and intracellular free calcium may play a key role in apoptotic T cell induced by ovarian carcinoma.
Wang
Hui
H
Department of Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Li
Xiao
X
Xie
Xing
X
Lü
Wei-guo
WG
Ye
Da-feng
DF
Chen
Huai-zeng
HZ
Cheng
Qi
Q
D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000097:blood; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000502:physiology; D010051:Ovarian Neoplasms / Q000276:immunology*
D009569:Nitric Oxide; D000242:Cyclic AMP; D002118:Calcium