Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.
Importin alpha, which mediates the nuclear import of nuclear localization signal (NLS)-containing proteins, is a member of nuclear transport factors. Importin alpha binds directly NLS and functions as an adapter for accessing the importin beta-dependent import pathway. To date, several isoforms of importin alpha have been identified and classified into three subfamilies in higher eukaryotes. In this study, we report on the production of a rat monoclonal antibody (MAb) against importin alpha3/Qip1, a member of the importin alpha family, using a rat medial iliac lymph node method. The MAb 3D10 produced, reacted with both recombinant and endogenous importin alpha 3/Qip1. Immunoblotting analysis revealed that MAb 3D10 exclusively recognizes importin alpha3/Qip1 among members of the importin alpha family, in various mammalian cells.
Sakaguchi
Naoko
N
Department of Applied & Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
Miyamoto
Yoichi
Y
Yoneda
Yoshihiro
Y
Ogino
Kenji
K
Tachibana
Taro
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D011485:Protein Binding; D020033:Protein Isoforms; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D028901:alpha Karyopherins / Q000737:chemistry*
D000911:Antibodies, Monoclonal; C106259:KPNA4 protein, human; D020033:Protein Isoforms; D011994:Recombinant Proteins; D028901:alpha Karyopherins