In vitro cytotoxicity of tanshinones isolated from Salvia miltiorrhiza Bunge against P388 lymphocytic leukemia cells.
The cytotoxic effect of four tanshinones isolated from the dried root of Salvia miltiorrhiza Bunge (Lamiaceae) were studied in vitro using P388 lymphocytic leukemia cells. Tanshinone I and tanshinone IIA were shown to be quite strongly cytotoxic against the cells (86.76% and 56.05% cell inhibition at 25 microg/ml). Dihydrotanshinone I and cryptotanshinone, which lack of saturation at C-15, showed little cytotoxicity (13.71% and 39.21% cell inhibition at 25 microg/ml). The results also revealed a structure activity relationship. We suggest that the unsaturation at C-15 and saturation on ring A may be critical structural components for cytotoxicity in these diterpenes.
Mosaddik
M A
MA
Centre for Phytochemistry, Southern Cross University, Lismore, NSW, Australia. mamosaddik@yahoo.com
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor / Q000187:drug effects; D007945:Leukemia, Lymphoid / Q000188:drug therapy; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D027561:Salvia miltiorrhiza; D013329:Structure-Activity Relationship
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts