Antibody-mediated neutralization of pertussis toxin-induced mitogenicity of human peripheral blood mononuclear cells.
Antibody-mediated neutralization of pertussis toxin-induced proliferation of human peripheral blood mononuclear cells (PBMC) was assessed using alamarBlue and compared with results from the Chinese hamster ovary (CHO) cell assay using sera from vaccinated adults and convalescent children. Neutralization values for the CHO assay were similar for vaccinated and convalescent subjects; however. the convalescent group had higher titers in the PBMC assay. Results for pertussis toxin neutralization with the CHO assay appear to be distinct from those with the PBMC assay.
Millen
Scott H
SH
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA.
Bernstein
David I
DI
Connelly
Beverly
B
Ward
Joel I
JI
Chang
Swei-Ju
SJ
Weiss
Alison A
AA
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D009500:Neutralization Tests; D037342:Pertussis Toxin / Q000276:immunology* / Q000494:pharmacology*
D000907:Antibodies, Bacterial; D037342:Pertussis Toxin