[Analysis of dose-dependent antibody titration curves].
Several types of dose-response titration curves were considered. It was demonstrated that the use of the so-called coordinates of dilution suggested earlier by us allows one to analyze the titration curves, obtained either by ELISA, or by agglutination. Theoretical curves, obtained by the developed theory are very similar to those obtained in experiments. It was shown, that the analysis of the titration curves could give important information concerning antibody-blocking factors in titration sera or other samples of studied antibodies.
Bobrovnik
S A
SA
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. sab@biochem.kiev.ua
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002417:Cattle; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology
D000906:Antibodies; D000907:Antibodies, Bacterial; D012710:Serum Albumin, Bovine; D010047:Ovalbumin