[Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis].
OBJECTIVE
To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.


METHODS
Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.


RESULTS
67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.


CONCLUSIONS
Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.
Jiang
Ping
P
General Hospital of Beijing Coal Mineral Bureau, Beijing 102308, China.
Li
Hongmin
H
Chen
Dongjin
D
Wang
Wei
W
Feng
Bai
B
Wang
Zhongyuan
Z
Wang
Guoyang
G
Li
Sumei
S
Han
Huixin
H
D003031:Coal; D024881:Drug Resistance, Bacterial; D006801:Humans; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012830:Silicotuberculosis / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology
D003031:Coal