title: Quantifying the effects of deuterium substitution on phospholipid mixing in bilayer membranes. A nearest-neighbor recognition investigation.
abstract: Nearest-neighbor recognition measurements have established that the effects of deuterium substitution on phospholipid mixing are exceedingly small. Thus, the mixing behavior of an exchangeable phospholipid bearing two stearoyl chains with a homologue containing two myristoyl chains in gel-fluid bilayers, fluid bilayers, cholesterol-rich fluid bilayers, and gel-fluid bilayers that have been enriched with cholesterol correspond to a difference in the free energy of mixing that is less than 2.2 cal/mol of hydrogen in all cases. These findings provide the strongest evidence to date in support of the use of deuterated phospholipids as "nonperturbing" probes for structural and dynamic studies of bilayer membranes.
authors.affiliation: Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Tokutake
authors.forename: Bingwen
authors.initials: B
authors.lastname: Jing
authors.forename: Honghua
authors.initials: H
authors.lastname: Cao
authors.forename: Steven L
authors.initials: SL
authors.lastname: Regen
mesh_terms: D003903:Deuterium; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009226:Myristates / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D009226:Myristates; D010743:Phospholipids; D003903:Deuterium