Determination of trace procaine hydrochloride by differential pulse adsorptive stripping voltammetry with a Nafion modified glassy carbon electrode.
A sensitive and selective method for the determination of procaine hydrochloride with a Nafion-modified glassy carbon electrode has been developed. The voltammetric behavior of procaine hydrochloride on the Nafion-modified electrode indicated that the modified electrode not only increased the sensitivity of the determination of procaine hydrochloride, but also catalyzed the electrode process. Procaine hydrochloride was accumulated in Britton-Robinson buffer (pH 2.09) at a potential of -0.2 V (vs. SCE) for 180 s, and was then determined by differential pulse adsorptive stripping voltammetry. The effect of various parameters, such as the pH of the medium, the mass of drop-coated Nafion, the accumulation potential, the accumulation time and the scan rate, were investigated. Under the optimum conditions, a linear calibration graph was obtained in the concentration range of 6.0 x 10(-8) to 6.0 x 10(-6) mol l(-1) with a correlation coefficient of 0.9987. The relative standard deviation was 4.18% for eight successive determinations of 1.0 x 10(-7) mol l(-1) procaine hydrochloride, and the detection limit (three times signal to noise) was 7.0 x 10(-9) mol l(-1). A study of interfering substances was also performed, and the method was applied to the direct determinations of procaine hydrochloride in the injection solution of procaine hydrochloride and in rabbit serum.
Li
Nianbing
N
Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, PR China.
Duan
Jianping
J
Chen
Guonan
G
D000779:Anesthetics, Local / Q000032:analysis / Q000097:blood; D000818:Animals; D002138:Calibration; D002244:Carbon; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D004566:Electrodes / Q000592:standards; D005465:Fluorocarbon Polymers; D011343:Procaine / Q000032:analysis* / Q000097:blood; D011817:Rabbits; D015203:Reproducibility of Results
D000779:Anesthetics, Local; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D011343:Procaine; D002244:Carbon