Protective effect of shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells.
The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.
Shin
Hyun-Taeg
HT
Department of Oriental Rehabilitation Medicine, College of Oriental Medicine Kyung Hee University, Seoul, Korea.
Chung
Seok-Hee
SH
Lee
Jong-Soo
JS
Kim
Sung-Soo
SS
Shin
Hyun-Dae
HD
Jang
Mi-Hyeon
MH
Shin
Min-Chul
MC
Bahn
Geon-Ho
GH
Paik
Eun-Kyung
EK
Park
Jae-Hyung
JH
Kim
Chang-Ju
CJ
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D006861:Hydrogen Peroxide; D009474:Neurons / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D013997:Time Factors
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D016194:Receptors, N-Methyl-D-Aspartate; C484999:shenqui-wan; D006861:Hydrogen Peroxide