[Development and identification of a set of Triticum aestivum-Thinopyrum bessarabicum disomic alien addition lines].
In order to transfer the genes for salt tolerance and disease resistance from Thinopyrum bessarabicum into wheat, the hybrid progenies between T. aestivum cv. Chinese Spring and T. aestivum cv. Chinese Spring-amphiploid Th. bessarabicum were screened. A set of T. aestivum-Th. bessarabicum disomic addition lines was developed with the assistance of mitotic chromosome C-banding and genomic in situ hybridization (GISH), as well as GISH on meiotic M I chromosome preparations. The results indicated that all the wheat chromosomes in the lines remained unchanged karyotypically, while the added Th. bessarabicum chromosomes paired regularly in meiosis. The developed disomic addition lines were designated temporarily as DAJ1, DAJ2, DAJ3, DAJ4, DAJ5, DAJ6 and DAJ7 respectively. Determination of the homoelogous groups of the added Th. bessarabicum chromosomes and localization of the genes for salt tolerance and disease resistance are undergoing.
Zhuang
Li-Fang
LF
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Institution of Cytogenetics, Nanjing Agricultural University, Nanjing 210095, China.
Qi
Zeng-Jun
ZJ
Ying
Jia
J
Chen
Pei-Du
PD
Liu
Da-Jun
DJ
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics; D006823:Hybrid Vigor / Q000235:genetics; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D006109:Poaceae / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000235:genetics*
D012965:Sodium Chloride