Galanin inhibits gut-related vagal neurons in rats.
Galanin plays an important role in the regulation of food intake, energy balance, and body weight. Many galanin-positive fibers as well as galanin-positive neurons were seen in the dorsal vagal complex, suggesting that galanin produces its effects by actions involving vagal neurons. In the present experiment, we used tract-tracing and neurophysiological techniques to evaluate the origin of the galaninergic fibers and the effect of galanin on neurons in the dorsal vagal complex. Our results reveal that the nucleus of the solitary tract is the major source of the galanin terminals in the dorsal vagal complex. In vivo experiments demonstrated that galanin inhibited the majority of gut-related neurons in the dorsal motor nucleus of the vagus. In vitro experiments demonstrated that galanin inhibited the majority of stomach-projecting neurons in the dorsal motor nucleus of the vagus by suppressing spontaneous activity and/or producing a fully reversible dose-dependent membrane hyperpolarization and outward current. The galanin-induced hyperpolarization and outward current persisted after synaptic input was blocked, suggesting that galanin acts directly on receptors of neurons in the dorsal motor nucleus of the vagus. The reversal potential induced by galanin was close to the potassium ion potentials of the Nernst equation and was prevented by the potassium channel blocker tetraethylammonium, indicating that the inhibitory effect of galanin was mediated by a potassium channel. These results indicate that the dorsal motor nucleus of the vagus is inhibited by galanin derived predominantly from neurons in the nucleus of the solitary tract projecting to the dorsal motor nucleus of the vagus nerve. Galanin is one of the neurotransmitters involved in the vago-vagal reflex.
Tan
Zhenjun
Z
Laboratory of Neurogastroenterology Research, Henry Ford Health System, Detroit, Michigan 48202, USA.
Fogel
Ronald
R
Jiang
Chunhui
C
Zhang
Xueguo
X
D000818:Animals; D004064:Digestive System / Q000166:cytology* / Q000187:drug effects / Q000294:innervation*; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D019004:Galanin / Q000494:pharmacology*; D007150:Immunohistochemistry; D007473:Ion Channels / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D010812:Physical Stimulation; D017729:Presynaptic Terminals / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000502:physiology; D013270:Stomach / Q000294:innervation / Q000502:physiology; D019789:Tetraethylammonium / Q000494:pharmacology; D014630:Vagus Nerve / Q000166:cytology* / Q000187:drug effects
D007473:Ion Channels; D019789:Tetraethylammonium; D019004:Galanin