title: Overexpression of the oncogenic kinase Pim-1 leads to genomic instability.
abstract: Aneuploidy and chromosomal aberrations are hallmarks of most human epithelial malignancies. Here we show that overexpression of the oncogenic kinase Pim-1 in human prostate epithelial cells induces genomic instability by subverting the mitotic spindle checkpoint. Cells overexpressing Pim-1 have a defect in the mitotic spindle checkpoint, abnormal mitotic spindles, centrosome amplification, and chromosome missegregation. Polyploidy and aneuploidy ensue due to a delay in completing cytokinesis. These results define a novel role for elevated Pim-1 expression in promoting genomic instability in human prostate tumors.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama 35294-0007, USA.
authors.forename: Meejeon
authors.initials: M
authors.lastname: Roh
authors.forename: Bernard
authors.initials: B
authors.lastname: Gary
authors.forename: Chisu
authors.initials: C
authors.lastname: Song
authors.forename: Nasser
authors.initials: N
authors.lastname: Said-Al-Naief
authors.forename: Albert
authors.initials: A
authors.lastname: Tousson
authors.forename: Andrew
authors.initials: A
authors.lastname: Kraft
authors.forename: Isam-Eldin
authors.initials: IE
authors.lastname: Eltoum
authors.forename: Sarki A
authors.initials: SA
authors.lastname: Abdulkadir
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D020090:Chromosome Segregation; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008297:Male; D008938:Mitosis / Q000235:genetics; D011123:Polyploidy; D011467:Prostate / Q000201:enzymology / Q000502:physiology; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051573:Proto-Oncogene Proteins c-pim-1; D008941:Spindle Apparatus / Q000235:genetics
chemical_list: D011518:Proto-Oncogene Proteins; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1