[Activity of the enzymes of carbon metabolism in Sulfobacillus sibiricus under various conditions of cultivation].
The thermoacidophilic iron-oxidizing chemolithotroph Sulfobacillus sibiricus N1T is characterized by steady growth and amplified cell yield when grown in vigorously aerated medium containing Fe2+, glucose, and yeast extract as energy sources. In this case, carbon dioxide, glucose, and yeast extract are used as carbon sources. Glucose is assimilated through the fructose-bisphosphate pathway and the pentose-phosphate pathway. Glyoxylate bypass does not function in S. sibiricus, and the tricarboxylic acid cycle is disrupted at the level of 2-oxoglutarate dehydrogenase. The presence of ribulose-bisphosphate carboxylase indicates that carbon dioxide fixation proceeds through the Calvin cycle. The activity of ribulose-bisphosphate carboxylase is highest in autotrophically grown cells. The cells also contain pyruvate carboxylase, phosphoenolpyruvate carboxylase, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate carboxytransphosphorylase.
Zakharchuk
L M
LM
Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
Egorova
M A
MA
Tsaplina
I A
IA
Bogdanova
T I
TI
Krasil'nikova
E N
EN
Melamud
V S
VS
Karavaĭko
G I
GI
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002952:Citric Acid Cycle; D003470:Culture Media; D005296:Ferrous Compounds; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005947:Glucose; D010427:Pentose Phosphate Pathway; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011766:Pyruvate Carboxylase / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
D003470:Culture Media; D005296:Ferrous Compounds; D002245:Carbon Dioxide; D002244:Carbon; D006597:Fructose-Bisphosphatase; D002262:Carboxy-Lyases; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D012273:Ribulose-Bisphosphate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D011766:Pyruvate Carboxylase; D005947:Glucose