title: Expression of Trk A receptors in the mammalian inner ear.
abstract: Tyrosine kinase receptors, including Trk A, Trk B and Trk C, participate in many different biological processes that are regulated by neurotrophic factors. Nerve growth factor (NGF)-triggered Trk A signaling is involved in growth, survival and differentiation of neurons in the central nervous system and in neural crest-derived cells. Trk A, Trk B and Trk C expression has been reported in the rat ventral cochlear nucleus. In the present study, we explored the immunocytochemical distribution of Trk A in the rodent inner ear. Rat and mouse cochleae were immunolabeled with a rabbit anti-Trk A polyclonal antibody (Chemicon) that has no reported cross-reactivity with Trk B and Trk C. In embryonic day 16 mice, no Trk A immunolabeling could be detected in the developing neuroepithelium. At postnatal day 6, weak Trk A labeling could be observed in both inner and outer hair cells. At postnatal day 12, enhanced punctate Trk A immunoexpression was present in hair cells. In adult mice and rats, intense Trk A labeling was observed in outer and inner hair cell bodies, in supporting cell bodies throughout the cochlea, and in spiral ganglion neurons. Trk A was not observed in stria vascularis, hair cell stereocilia, nor in the Trk B- and Trk C-rich cerebellum. This distribution pattern of Trk A suggests that its ligand, NGF, exerts significant trophic effects in the rodent inner ear.
authors.affiliation: Oregon Hearing Research Center, NRC04, Department of Otolaryngology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: Dai
authors.forename: P S
authors.initials: PS
authors.lastname: Steyger
authors.forename: Z M
authors.initials: ZM
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Vass
authors.forename: A L
authors.initials: AL
authors.lastname: Nuttall
mesh_terms: D000818:Animals; D007758:Ear, Inner / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D051381:Rats; D020917:Receptor, trkA / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D020917:Receptor, trkA