Dynamics of the IRE RNA hairpin loop probed by 2-aminopurine fluorescence and stochastic dynamics simulations.
The iron responsive element (IRE) RNA hairpin loop contains six phylogenetically conserved nucleotides, which constitute part of the sequence-specific binding site of the IRE-binding protein. The NMR structure of the loop has been solved, showing that 3 of the 6 nt are poorly constrained. Here, two purine nucleotides in the IRE loop are individually replaced with the fluorescent purine analog 2-aminopurine (2AP). Steady-state and time-resolved fluorescence methods are used to describe the structure and dynamics of 2AP in the IRE loop. The data indicate that 2AP at the position of the adenosine in the loop moves between stacked and unstacked positions, whereas 2AP at the adjacent guanosine is predominantly solvent exposed. Stochastic dynamics simulations are used to provide a physical description of how those nucleotides might move.
Hall
Kathleen B
KB
Department of Biochemistry & Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA. Divergence, Inc., St. Louis, Missouri 63141, USA. hall@bionmr3.wustl.edu
Williams
D Jeremy
DJ
D015075:2-Aminopurine / Q000737:chemistry*; D006801:Humans; D035925:Iron-Regulatory Proteins / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D035925:Iron-Regulatory Proteins; D009692:Nucleic Acid Heteroduplexes; D015075:2-Aminopurine; D012313:RNA