title: Essential role for ADAM19 in cardiovascular morphogenesis.
abstract: Congenital heart disease is the most common form of human birth defects, yet much remains to be learned about its underlying causes. Here we report that mice lacking functional ADAM19 (mnemonic for a disintegrin and metalloprotease 19) exhibit severe defects in cardiac morphogenesis, including a ventricular septal defect (VSD), abnormal formation of the aortic and pulmonic valves, leading to valvular stenosis, and abnormalities of the cardiac vasculature. During mouse development, ADAM19 is highly expressed in the conotruncus and the endocardial cushion, structures that give rise to the affected heart valves and the membranous ventricular septum. ADAM19 is also highly expressed in osteoblast-like cells in the bone, yet it does not appear to be essential for bone growth and skeletal development. Most adam19(-/-) animals die perinatally, likely as a result of their cardiac defects. These findings raise the possibility that mutations in ADAM19 may contribute to human congenital heart valve and septal defects.
authors.affiliation: Cell Biology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Zhou
authors.forename: Gisela
authors.initials: G
authors.lastname: Weskamp
authors.forename: Valérie
authors.initials: V
authors.lastname: Chesneau
authors.forename: Umut
authors.initials: U
authors.lastname: Sahin
authors.forename: Andrea
authors.initials: A
authors.lastname: Vortkamp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Horiuchi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Chiusaroli
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hahn
authors.forename: David
authors.initials: D
authors.lastname: Wilkes
authors.forename: Peter
authors.initials: P
authors.lastname: Fisher
authors.forename: Roland
authors.initials: R
authors.lastname: Baron
authors.forename: Katia
authors.initials: K
authors.lastname: Manova
authors.forename: Craig T
authors.initials: CT
authors.lastname: Basson
authors.forename: Barbara
authors.initials: B
authors.lastname: Hempstead
authors.forename: Carl P
authors.initials: CP
authors.lastname: Blobel
mesh_terms: D051722:ADAM Proteins; D000818:Animals; D015153:Blotting, Western; D001846:Bone Development / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002319:Cardiovascular System / Q000196:embryology*; D019483:Disintegrins / Q000378:metabolism*; D008168:Lung / Q000254:growth & development; D008565:Membrane Proteins / Q000378:metabolism*; D045726:Metalloproteases / Q000378:metabolism*; D051379:Mice
chemical_list: D019483:Disintegrins; D008565:Membrane Proteins; D045726:Metalloproteases; D051722:ADAM Proteins; C096116:Adam19 protein, mouse