title: Association of interleukin-18 gene promoter polymorphisms in type 1 diabetes and autoimmune thyroid disease.
abstract: Type 1 diabetes is a heterogeneous autoimmune disease and is often associated with other organ-specific autoimmune diseases, including autoimmune thyroid disease (AITD). IL-18 is a potent proinflammatory cytokine capable of inducing IFN-gamma production that is associated with the development of type 1 diabetes and AITD. The gene for IL-18 is located near Idd2 and has been reported to be associated with a susceptibility to type 1 diabetes. To test the putative involvement of IL-18 gene polymorphism in predisposition to type 1 diabetes and AITD, we conducted a case-control study in Japanese population. The SNPs at position -607 (C/A) and -137 (G/C) in the promoter region of the IL-18 gene were analyzed by sequence-specific PCR in 74 nondiabetic patients with AITD, 47 type 1 diabetic patients with AITD, and 114 normal controls. There was no significant increase in the genotype and allele frequencies not only in nondiabetic patients with AITD compared with normal controls, but also in type 1 diabetic patients with AITD compared with normal controls. The distribution of IL-18 gene haplotypes was also similar between both patient groups and normal controls. These results suggest that polymorphisms of the IL-18 gene are not associated with a susceptibility to AITD and type 1 diabetes coexistent with AITD in Japanese population.
authors.affiliation: First Department of Internal Medicine, Graduate School of Biochemical Sciences, Nagasaki University School of Medicine, Nagasaki, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Ide
authors.forename: Eiji
authors.initials: E
authors.lastname: Kawasaki
authors.forename: Norio
authors.initials: N
authors.lastname: Abiru
authors.forename: Fuyan
authors.initials: F
authors.lastname: Sun
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukushima
authors.forename: Reiko
authors.initials: R
authors.lastname: Ishii
authors.forename: Ryoko
authors.initials: R
authors.lastname: Takahashi
authors.forename: Hironaga
authors.initials: H
authors.lastname: Kuwahara
authors.forename: Naruhiro
authors.initials: N
authors.lastname: Fujita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kita
authors.forename: Misa
authors.initials: M
authors.lastname: Imaizumi
authors.forename: Katsuya
authors.initials: K
authors.lastname: Oshima
authors.forename: Toshiro
authors.initials: T
authors.lastname: Usa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Uotani
authors.forename: Eri
authors.initials: E
authors.lastname: Ejima
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Ashizawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Katsumi
authors.initials: K
authors.lastname: Eguchi
mesh_terms: D000328:Adult; D001327:Autoimmune Diseases / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020382:Interleukin-18 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D013959:Thyroid Diseases / Q000235:genetics*
chemical_list: D020382:Interleukin-18