Expression of binary toxin genes in the mosquito-colonizable bacteria, Bacillus cereus, leads to high toxicity against Culex quinquefasciatus larvae.
Two B. cereus strains, Ae10 and Cx5, isolated from mosquito larval guts, were transformed with a recombinant plasmid, pBS373, harboring binary toxin genes from Bacillus sphaericus 2297. Immunoblotting analysis clearly revealed the production and presence of the 51-kDa toxin protein in both strains. Two recombinant B. cereus strains Ae10 and Cx5 showed very high toxicity against C. quinquefasciatus larvae. Since both strains have a close relationship with the mosquito larvae in the native environment and are capable of recolonizing in the guts of mosquito larvae, these strains can be considered promising new hosts for an effective delivery of mosquito-larvicidal toxins.
Luxananil
Plearnpis
P
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand. pleam@biotec.or.th
Tanapongpipat
Sutipa
S
Promdonkoy
Boonhiang
B
Atomi
Haruyuki
H
Imanaka
Tadayuki
T
Panyim
Sakol
S
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics* / Q000633:toxicity; D003001:Cloning, Molecular; D003465:Culex / Q000254:growth & development* / Q000382:microbiology*; D015870:Gene Expression; D015151:Immunoblotting / Q000379:methods; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D010572:Pest Control, Biological / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000633:toxicity; D014169:Transformation, Bacterial
D001427:Bacterial Toxins; D007306:Insecticides; D011994:Recombinant Proteins; C046134:binB protein, Bacillus sphaericus