title: CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility.
abstract: BACKGROUND
  The search for Ca2+ channels residing in sperm has led to the recent cloning and characterization of a novel gene, named CatSper, which codes for a unique Ca2+ channel expressed exclusively in the testis. It plays an essential role in sperm motility, penetration into the oocyte, and ultimately in male fertility. In this study, we assessed the temporal profile of CatSper gene expression during mouse testis development and performed a semi-quantitative evaluation of expression levels in a group of subfertile men which lack sperm motility.
  
  
  METHODS
  A small piece of testicular tissue obtained by either multi-site testicular biopsy or orchidectomy was used for semi-quantitative RT-PCR of CatSper and beta2-microglobulin (beta2m, as an internal control) genes.
  
  
  RESULTS
  Our results reveal that: (i) the expression of mouse CatSper is developmentally regulated with a direct correlation between CatSper expression and mouse sexual maturation. CatSper gene expression is first detected at 3 weeks of age and coincides with the appearance of round spermatids in the developing mouse testis. (ii) There is a significant reduction in the level of CatSper gene expression (up to 3.5-fold difference) among patients which lack sperm motility as compared with patients whose infertility cannot be ascribed to a deficiency in motility (used as a control).
  
  
  CONCLUSIONS
  The data obtained in this study support a potential role for CatSper in sperm motility and fertility in mouse and human. CatSper is therefore implicated as a potential target to explore the molecular mechanisms of male infertility.
authors.affiliation: Department of Genetics, Faculty of Basic Sciences, Tarbiat Modarres University, Tehran, 14115-175, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Nikpoor
authors.forename: S Javad
authors.initials: SJ
authors.lastname: Mowla
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Movahedin
authors.forename: S Amir-Mohsen
authors.initials: SA
authors.lastname: Ziaee
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015870:Gene Expression; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D051379:Mice / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012741:Sexual Maturation; D013081:Sperm Motility; D013737:Testis / Q000254:growth & development* / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism
chemical_list: C438823:CATSPER1 protein, human; D015220:Calcium Channels; C488642:Catsper1 protein, mouse; D001613:beta 2-Microglobulin