title: Deregulated expression of KRAP, a novel gene encoding actin-interacting protein, in human colon cancer cells.
abstract: We have identified a novel gene, designated KRAP (Ki- ras-induced actin-interacting protein), encoding a protein of 1,259 amino acids with coiled-coil regions and transmembrane regions, from the cDNA library of human colon cancer HCT116 cells, as one of the genes upregulated by activated Ki- ras. While KRAP was rarely expressed in normal colon epithelium, deregulated constitutive KRAP expression was observed in some other colon cancer cells. In normal tissues, KRAP was strongly expressed in pancreas and testis. Anti-KRAP polyclonal antibodies detected endogenous KRAP as the molecular size of Mr 180,000, and immunofluorescence microscopy and cytochalasin E treatment revealed that KRAP was clearly associated with the actin filaments. Furthermore, KRAP was localized as a membrane-bound form with extracellular regions. These results together suggested KRAP might be involved in the regulation of filamentous actin and signals from the outside of the cells.
authors.affiliation: Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.|Department of Urology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Inokuchi
authors.affiliation: Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Komiya
authors.affiliation: Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Iwai
authors.initials: I
authors.lastname: Baba
authors.affiliation: Department of Urology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Naito
authors.affiliation: Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sasazuki
authors.affiliation: Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan. sshirasawa@ri.imcj.go.jp.
authors.forename: Senji
authors.initials: S
authors.lastname: Shirasawa
mesh_terms: D000595:Amino Acid Sequence; D019921:Biotinylation; D015152:Blotting, Northern; D003572:Cytochalasins; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D015723:Gene Library; D011905:Genes, ras / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism; D011233:Precipitin Tests; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013737:Testis / Q000378:metabolism; D014162:Transfection
chemical_list: D003572:Cytochalasins; C482653:ITPRID2 protein, human; D008565:Membrane Proteins; D008840:Microfilament Proteins; C007215:cytochalasin E