Temperature may influence and regulate NF-YB expression in toad oocyte.
Fully grown oocytes derived from Bufo gargarizans maintained at relatively low temperatures (4 degrees C, LTE-oocytes) acquire the competence to resume normal meiosis. In contrast, fully grown oocytes derived from toads maintained at relatively high temperatures (28 degrees C, HTE-oocytes) never acquire maturation competence. By suppression subtractive hybridization, we obtained 18 ESTs preliminarily thought to be preferentially expressed in LTE-oocytes; of these, TS1-4 shared homology with the human and mouse NF-YB genes. We cloned the full-length toad NF-YB gene by RACE and identified three alternatively spliced transcripts: tNF-YB1, tNF-YB2, and tNF-YB3 (GenBank Accession Nos. AY442015, AY442016, and AY442017, respectively). Toad NF-YB was differentially transcribed and translated in LTE-oocytes versus HTE-oocytes, likely resulting in differential CCAAT-binding and/or transcriptional activity of NF-Y. Furthermore, toad cyclin B2 was differentially transcribed at high and low temperatures. Taken together, this report of the differential expression of toad NF-YB at different temperatures is the first evidence that temperature may influence and regulate NF-YB expression.
Yan
Shan
S
Shanghai Medical College, Fudan University, Shanghai 200032, PR China. yanshan007@yahoo.com.cn
Tso
Jiake
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002025:Bufonidae; D023081:CCAAT-Binding Factor / Q000096:biosynthesis* / Q000737:chemistry; D003001:Cloning, Molecular; D019926:Cyclin B / Q000378:metabolism; D056765:Cyclin B2; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D020224:Expressed Sequence Tags; D008540:Meiosis; D008954:Models, Biological; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism*; D016366:Open Reading Frames; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000096:biosynthesis*; D014158:Transcription, Genetic
D023081:CCAAT-Binding Factor; C503758:CCNB2 protein, human; C503759:Ccnb2 protein, mouse; D019926:Cyclin B; D056765:Cyclin B2; D004268:DNA-Binding Proteins; C492063:NFYB protein, human; C492062:Nfyb protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors