title: An oriented peptide array library (OPAL) strategy to study protein-protein interactions.
abstract: One of the major questions in signal transduction is how the specificities of protein-protein interactions determine the assembly of distinct signaling complexes in response to stimuli. Several peptide library methods have been developed and widely used to study protein-protein interactions. These approaches primarily rely on peptide or DNA sequencing to identify the peptide or consensus motif for binding and may prove too costly or difficult to accommodate high throughput applications. We report here an oriented peptide array library (OPAL) approach that should facilitate high throughput proteomic analysis of protein-protein interactions. OPAL integrates the principles of both the oriented peptide libraries and array technologies. Hundreds of pools of oriented peptide libraries are synthesized as amino acid scan arrays. We demonstrate that these arrays can be used to map the specificities of a variety of interactions, including antibodies, protein domains such Src homology 2 domains, and protein kinases.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Shawn S-C
authors.initials: SS
authors.lastname: Li
authors.forename: J Wade
authors.initials: JW
authors.lastname: Harper
authors.forename: Zhou
authors.initials: Z
authors.lastname: Songyang
mesh_terms: D000818:Animals; D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D019151:Peptide Library; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D018909:src Homology Domains
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D019151:Peptide Library