Preservation of human saphenous vein against reactive oxygen species-induced oxidative stress by green tea polyphenol pretreatment.
The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.
Han
Dong-Wook
DW
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
Suh
Hwal
H
Park
Young Hwan
YH
Cho
Bum Koo
BK
Hyon
Suong-Hyu
SH
Park
Jong-Chul
JC
D000975:Antioxidants / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D012501:Saphenous Vein / Q000187:drug effects; D013662:Tea; D014652:Vascular Diseases / Q000209:etiology / Q000517:prevention & control
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013662:Tea