title: Ternary complex formation between DNA-adenovirus core protein VII and TAF-Ibeta/SET, an acidic molecular chaperone.
abstract: The adenovirus (Ad) genome complexed with viral core proteins designated Ad core is the template for transcription of early genes and the first round of replication in Ad-infected cells. A cellular protein designated template-activating factor-I (TAF-I) is found to be involved in remodeling of the Ad core in vitro. Here we found that TAF-I interacts with the Ad DNA through core protein VII in infected cells in early phases of infection. In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA-protein VII complexes.
authors.affiliation: Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Haruki
authors.forename: Bela
authors.initials: B
authors.lastname: Gyurcsik
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Okuwaki
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D011232:Chemical Precipitation; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins; D024202:Electrophoretic Mobility Shift Assay; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism / Q000821:virology; D005814:Genes, Viral; D056488:Histone Chaperones; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C513473:SET protein, human; D014157:Transcription Factors; D014758:Viral Core Proteins