[Effects of daidzein on sperm quality, testis gain and testosterone in mice].
OBJECTIVE
To evaluate the effects of daidzein on sperm quality of male mice.


METHODS
Three different doses of daidzein were supplemented to pubertal male mice for 21 days(5 mg/kg, 100 mg/kg, 1,000 mg/kg ration). The viability of sperm was determined by eosin-Y, the acrosome was observed by Wright-Giemsa staining, and the testosterone was measured by radioimmunoassay.


RESULTS
Daidzein at the dose of 5 mg/kg ration significantly increased serum testosterone levels (P < 0.01), prompted testis gain (P < 0.05), and improved spermatozoa quality. Daidzein at dose of 1,000 mg/kg ration could inhibit the secretion of serum testosterone (P < 0.01), without significant variation in spermatozoa quality. Daidzein at dose of 100 mg/kg ration did not significantly affect sperm quality and other index.


CONCLUSIONS
Daidzein can affect sperm quality and in dosage-dependant ways.
Han
Zhao-Yu
ZY
College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Wang
Gen-Lin
GL
Chen
Wen
W
Zhang
Li-Jun
LJ
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D048789:Phytoestrogens / Q000494:pharmacology*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*; D013737:Testis / Q000187:drug effects*; D013739:Testosterone / Q000097:blood*
D007529:Isoflavones; D048789:Phytoestrogens; D013739:Testosterone; C004742:daidzein