Frequent loss of heterozygosity on chromosome 9 in Chinese esophageal squamous cell carcinomas.
Multiple and extensive alterations in chromosome 9 were detected in thirty-four esophageal squamous cell carcinoma patients, using seventeen polymorphic markers localized to chromosome 9 to detect the loss of heterozygosity (LOH) by polymerase chain reaction techniques. The LOH rates detected in this study range from 42.9 to 80.0%. Three commonly deleted regions mapping to 9p23-p22, 9q13-q22.3, and 9q34 were observed. D9S1812 LOH at 9q22.1 was significantly associated with well- and moderately-differentiated tumors; LOH at D9S768, mapping to 9q13-21.3, indicated that drinking habits are not a significant risk factor for Chinese esophagus cancer. Interestingly, no case of microsatellite instability was observed.
Lichun
Yang
Y
Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong (SAR), Kowloon, People's Republic of China.
Ching Tang
Cecilia Man
CM
Wai Lau
Kwok
K
Lung
Maria Li
ML
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002681:China; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction