Rapid suppression of testosterone secretion after capture in male American alligators (Alligator mississippiensis).
All reptiles studied to date show an increase in circulating corticosterone following capture. This rise in corticosterone has also been shown in a number of instances to result in a decline in reproductive steroids within hours after capture. As a result of these observations it has been considered imperative to collect blood samples as soon as possible after capture to get reliable measures of reproductive hormones. It has been claimed, however, that there is no effect of capture stress on reproductive steroids in juvenile alligators held for 2 h following capture. As we generally reject blood samples that are not collected within 15 min of capture we decided to reinvestigate the effect of short-term capture (2 h) on corticosterone and testosterone in male alligators. Four groups of alligators, ranging in size from 74 to 212 cm total length were captured in a 2-week period in May, the time of year when testosterone levels are highest. Two groups were captured during the day (eight bled at capture and again at 2 h, eight bled at 2 h only) and two at night (10 bled at capture and again at 2 h, 10 bled at 2 h only). Testosterone and corticosterone in alligators bled immediately on capture and at 2 h were not significantly different in the AM and PM samples so the results were combined (Initial bleed: corticosterone, 0.95 +/- 0.09 ng/ml, n=18; testosterone, 6.06 +/- 2.09 ng/ml, n=18. Two-hour bleed: corticosterone 15.68 +/- 1.91, n=18; testosterone, 2.75 +/- 0.79, n=18). Both the increase in corticosterone and the decline in testosterone at 2 h were significant (p<0.05). Corticosterone and testosterone in the alligators sampled only once at 2 h were not significantly different from the 2-h values in alligators sampled twice (corticosterone 15.04 +/- 1.29, n=18; testosterone, 1.85 +/- 0.62, n=18). These results clearly demonstrate that short-term capture stress results in a significant decline in testosterone in male alligators.
Lance
Valentine A
VA
Center for Reproduction of Endangered Species, PO Box 120551, San Diego, CA 92112, USA. lvalenti@sunstroke.sdsu.edu
Elsey
Ruth M
RM
Butterstein
George
G
Trosclair
Phillip L
PL
D000489:Alligators and Crocodiles / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose; D001798:Blood Proteins / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D006234:Handling, Psychological; D008297:Male; D012149:Restraint, Physical; D012621:Seasons; D013312:Stress, Physiological / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D001786:Blood Glucose; D001798:Blood Proteins; D013739:Testosterone; D003345:Corticosterone