[Effect of polydatin on lipopolysaccharide-induced leucocyte chemotaxis].
OBJECTIVE
To understand the action mechanisms of polydatin (PD) in the treatment of septic shock in view of its effect on lipopolysaccharide (LPS)-induced chemotaxis of the leucocytes.


METHODS
Chemotactic chamber assay was used to investigate the regulative role of PD in chemotaxis of the neutrophils in response to LPS stimulation.


RESULTS
The chemotactic index of normal neutrophils was 4.96+/-0.69, which was significantly increased by LPS stimulation. LPS stimulation at the doses of 10, 100, and 1000 ng/ml resulted in the elevation of the chemotactic index of the neutrophils to 8.94+/-1.73, 10.31+/-1.180 and 7.12+/-1.46 respectively (P<0.05), an effect potently reversed by the application of PD at the concentrations ranging from 0.008 to 0.8 mg/ml, of which 0.08 mg/ml was the most effective concentration that produced a decrease of the chemotactic index to 1.95+/-0.17. In addition, PD exhibited obvious anti-LPS effect after treatment for 5 to 60 min (P<0.05), while showing no influence on the chemotaxis of normal neutrophils (P>0.05).


CONCLUSIONS
PD can regulate neutrophil chemotaxis in inflammatory reactions and may play a crucial role in the treatment of infections and inflammation.
Huang
Hai-xiao
HX
Key Laboratory of Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China.
Zhao
Qing
Q
Jin
Chun-hua
CH
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000494:pharmacology*
D005960:Glucosides; D008070:Lipopolysaccharides; D013267:Stilbenes; C058229:polydatin