Elevation of serum hepatocyte growth factor during granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization.
We examined serum levels of the angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hepatocyte growth factor (HGF), in normal donors for allogeneic peripheral blood stem cell (PBSC) transplantation. Granulocyte colony-stimulating factor (G-CSF) (filgrastim 400 microg/m2/d) was administered to 23 donors for 5 d and aphereses were performed on days 4 and 5. Although bFGF remained at similar levels after G-CSF treatment, serum VEGF and HGF levels increased 1.5-fold (n = 13; P = 0.02) and 6.8-fold (n = 23; P < 0.0001) respectively. The serum HGF level before G-CSF administration on day 1 correlated inversely with mobilized CD34+ cell numbers. Time course kinetics of HGF showed that on the day after G-CSF administration (day 2), serum HGF levels increased to 3678 pg/ml. For auto PBSC mobilization with chemotherapy and G-CSF 200 microg/m2/d (n = 8), we observed similar HGF elevation, which appeared to be dose-dependent on the G-CSF administered.
Fujii
Keiko
K
Department of Medicine, University of Okayama, 2-5-1 Shikatacho, Okayama 700-8558, Japan.
Ishimaru
Fumihiko
F
Kozuka
Teruhiko
T
Matsuo
Keitaro
K
Nakase
Koichi
K
Kataoka
Itaru
I
Tabayashi
Takayuki
T
Shinagawa
Katsuji
K
Ikeda
Kazuma
K
Harada
Mine
M
Tanimoto
Mitsune
M
D000970:Antineoplastic Agents / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000097:blood*; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006402:Hematologic Diseases / Q000097:blood / Q000628:therapy*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D017228:Hepatocyte Growth Factor / Q000097:blood*; D006801:Humans; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D016179:Granulocyte Colony-Stimulating Factor; D017228:Hepatocyte Growth Factor; D000069585:Filgrastim