Effect of drying methods on hybrid layer thickness.
OBJECTIVE
To evaluate the effect of drying methods used in scanning electron microscopy on the thickness of the hybrid layers created by several different adhesive systems.


METHODS
A total etch dry bonding system, (Photobond), a three-step bonding adhesive system, (Scotchbond Multi-Purpose), a wet-bonding adhesive system (All-Bond 2) and a self-etching primer system (Mac Bond II) were used in this study. To permit measurement of the thickness of the hybrid layer, the resin-dentin interfaces were polished and etched briefly to remove polishing debris followed by immersion in 10% NaOCl for 4 hours. Half of the specimens in each group were subjected to fixation and critical-point drying followed by gold sputter-coating. The remaining specimens were allowed to air dry prior to gold sputter-coating. SEM observations were carried out to determine the structure and thickness of the hybrid layers.


RESULTS
The thickness of hybrid layers were affected by the drying methods with the critical-point drying methods producing the thickest hybrid layers (P < 0.05). The thickest hybrid layers were created by Photobond, with Scotchbond Multi-Purpose and All-Bond 2 producing intermediate thickness hybrid layers, among the total etch systems. The thinnest hybrid layer among all of the adhesive systems was produced by the self-etching primer, Mac Bond II.
Itou
Kousuke
K
Department of Operative Dentistry, Field of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine and Dentistry, Japan.
Torii
Yasuhiro
Y
Oyama
Fumiko
F
Yoshiyama
Masahiro
M
Pashley
David H
DH
D000134:Acid Etching, Dental; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003890:Desiccation; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D016628:Replica Techniques; D019279:Resin Cements / Q000737:chemistry; D013499:Surface Properties; D016707:Tissue Fixation / Q000379:methods
C112828:Clearfil AP-X; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C452442:Mac Bond II; D008689:Methacrylates; C481355:Photobond; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C100742:All-Bond 2; D017438:Bisphenol A-Glycidyl Methacrylate