title: [Activity of the enzymes of carbon metabolism in Sulfobacillus sibiricus under various conditions of cultivation].
abstract: The thermoacidophilic iron-oxidizing chemolithotroph Sulfobacillus sibiricus N1T is characterized by steady growth and amplified cell yield when grown in vigorously aerated medium containing Fe2+, glucose, and yeast extract as energy sources. In this case, carbon dioxide, glucose, and yeast extract are used as carbon sources. Glucose is assimilated through the fructose-bisphosphate pathway and the pentose-phosphate pathway. Glyoxylate bypass does not function in S. sibiricus, and the tricarboxylic acid cycle is disrupted at the level of 2-oxoglutarate dehydrogenase. The presence of ribulose-bisphosphate carboxylase indicates that carbon dioxide fixation proceeds through the Calvin cycle. The activity of ribulose-bisphosphate carboxylase is highest in autotrophically grown cells. The cells also contain pyruvate carboxylase, phosphoenolpyruvate carboxylase, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate carboxytransphosphorylase.
authors.affiliation: Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
authors.forename: L M
authors.initials: LM
authors.lastname: Zakharchuk
authors.forename: M A
authors.initials: MA
authors.lastname: Egorova
authors.forename: I A
authors.initials: IA
authors.lastname: Tsaplina
authors.forename: T I
authors.initials: TI
authors.lastname: Bogdanova
authors.forename: E N
authors.initials: EN
authors.lastname: Krasil'nikova
authors.forename: V S
authors.initials: VS
authors.lastname: Melamud
authors.forename: G I
authors.initials: GI
authors.lastname: Karavaĭko
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002952:Citric Acid Cycle; D003470:Culture Media; D005296:Ferrous Compounds; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005947:Glucose; D010427:Pentose Phosphate Pathway; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011766:Pyruvate Carboxylase / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
chemical_list: D003470:Culture Media; D005296:Ferrous Compounds; D002245:Carbon Dioxide; D002244:Carbon; D006597:Fructose-Bisphosphatase; D002262:Carboxy-Lyases; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D012273:Ribulose-Bisphosphate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D011766:Pyruvate Carboxylase; D005947:Glucose