A newborn rat model for the study of cerebral hemodynamics by near-infrared spectroscopy and laser-Doppler flowmetry in the immature brain.
An animal model for the study of cerebrovascular physiology in the immature brain was developed. Twelve 3- to 5-day-old rat pups were maintained on spontaneous breathing under light anesthesia for either 1 or 2 h. Transcutaneous carbon dioxide tension and arterial oxygen saturation were monitored. Continuous infusion of doxapram limited respiratory acidosis. Cerebral blood flow (CBF) and volume (CBV) could be monitored by near-infrared spectroscopy (NIRS) and laser-Doppler flowmetry (LDF) in spite of some movement artifacts. CBV and CBF were 6.0 +/- 0.3 SE ml/100 g and 36.3 +/- 3.1 SE ml/100 g/min, respectively, and remained stable during the study. Cerebrovascular responses, as monitored by LDF and NIRS, to hypoxic and hypercapnic gas mixtures were consistent.
Fumagalli
Monica
M
Department of Neonatology, Clinica Mangiagalli I.C.P. Milan, University of Milan, Milan, Italy. monica.fumagalli@neonatologia-icp.it
Mosca
Fabio
F
Moos Knudsen
Gitte
G
Greisen
Gorm
G
D000136:Acid-Base Equilibrium; D000280:Administration, Inhalation; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001810:Blood Volume; D002245:Carbon Dioxide / Q000008:administration & dosage / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000187:drug effects; D005240:Feasibility Studies; D006439:Hemodynamics; D006706:Homeostasis; D000860:Hypoxia / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry; D023421:Models, Animal; D010313:Partial Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared
D002245:Carbon Dioxide