[Difference in lymphocyte heat shock protein 71 (HSP71) expression in soldiers of armed police forces from different climatic zones].
OBJECTIVE
To analyze the difference between basal and heat-inducible levels of lymphocyte heat shock protein 71 (HSP71) expression in soldiers from Beijing, Zhengzhou and Guangzhou.


METHODS
Flow cytometry and Comet assay were used to detect the level of HSP71 and DNA damage respectively.


RESULTS
Comet assay showed that there was no significant DNA damage before and after heat stress at 41 degrees C for 1 h, and also no difference found among the 3 climatic zones(P > 0.05). HSP71 of all soldiers in the 3 zones elevated after stress (P < 0.05). The basal and heat-inducible levels of HSP71 in Beijing soldiers(845.87 +/- 135.60 and 1254. 47 +/- 239.05 mean fluorescence intensity respectively) were higher than those in Guangzhou soldiers(702.73 +/- 184.70 and 861.72 +/- 225.12 mean fluorescence intensity respectively) (P < 0.05).


CONCLUSIONS
The differences of lymphocyte HSP71 expression before and after heat stress among the soldiers from Beijing, Zhengzhou and Guangzhou suggest that basal and heat-inducible levels of lymphocyte HSP71 expression may be considered as a valuable index to evaluate heat tolerance of soldiers in different climatic zones.
Zeng
Huasong
H
Chengdu Wujing Hospital, Chengdu 610000, China.
Xiao
Chengfeng
C
Jing
Xiujing
X
Yang
Miao
M
Ke
Lei
L
Xu
Qian
Q
Tan
Hao
H
Li
Fangze
F
Wu
Tangchun
T
D002980:Climate; D020552:Comet Assay; D004249:DNA Damage; D005434:Flow Cytometry; D018882:Heat Stress Disorders / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008889:Military Personnel; D016495:Police
D006360:Heat-Shock Proteins