Purification of saliva agglutinin of Streptococcus intermedius and its association with bacterial aggregation and adherence.
Streptococcus intermedius strain 1208-1 cells were aggregated in the presence of saliva. The saliva agglutinin was purified by centrifugation, filtration, and gel filtration. SDS-PAGE analyses indicated that the purified agglutinin consisted of two high-molecular-mass proteins. Aggregation was dependent on calcium over pH 5.5, with 1 mM being the most effective concentration. Boiling inactivated purified agglutinin. S. intermedius strain 3 and Streptococcus mutans strain 1 were aggregated in the purified agglutinin. After adsorption with strain 1208-1 cells, the saliva sample did not exhibit any aggregation activity, and the agglutinin bands were no longer visible by SDS-PAGE. Adherence analyses demonstrated that the purified agglutinin immobilized on the surfaces of polystyrene wells, actinomyces cells, and apatite beads accounted for the binding of streptococcus cells. Agglutinin also effectively inhibited adherence to apatite beads coated with native saliva.
Yamaguchi
Taihei
T
Department of Preventive Dentistry, Kagoshima University Dental School, 8-35-1, Sakuragaoka, 890-8544 Kagoshima, Japan. yamatai@dentb.hal.kagoshima-u.ac.jp
D000190:Actinomyces; D000373:Agglutinins / Q000302:isolation & purification* / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D002122:Calcium Chloride / Q000494:pharmacology; D002498:Centrifugation; D002850:Chromatography, Gel; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011137:Polystyrenes; D012463:Saliva / Q000737:chemistry*; D034367:Streptococcus intermedius / Q000502:physiology*
D000373:Agglutinins; D011137:Polystyrenes; D017886:Durapatite; D002122:Calcium Chloride