[Changes of peripheral blood CD45RA+ cells after hematopoietic stem cell transplantation: evaluation with four-color flow cytometry].
OBJECTIVE
To investigate the alteration of peripheral blood naive T cells in adult patients after hematopoietic stem cell transplantation (HSCT).


METHODS
Peripheral blood samples were collected from 49 patients requiring stem cell transplantation before and 15, 30, 90, and 180 d after transplantation, respectively. Dynamic changes of CD4(+)CD45RA(+)/CD8(+)CD45RA(+) cell counting were measured using flow cytometry.


RESULTS
Flow cytometry results of 90 samples showed that CD8(+)CD45RA(+) cells recovered to normal level 30 d after transplantation, whereas CD4(+)CD45RA(+) cells remained low and kept a slow pace of gradual increase over the 6 months following the transplantation. More rapid recovery of the transport and regeneration function of the thymus cells was achieved in patients receiving hematopoietic stem cell autotransplant than in those with allotransplant.


CONCLUSIONS
Measurement of the changes of CD4(+)CD45RA(+)/CD8(+)CD45RA(+) cell ratio using flow cytometry may provide insights into the molecular picture of the thymus regeneration function in association with immune reconstruction process in patients after stem cell transplantation. The number of newly generated T cells transported from the thymus can be a putative marker of immune reconstruction after transplantation.
Yin
Ji-xia
JX
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Sun
Jin
J
Yi
Zheng-shan
ZS
Zhou
Shu-yun
SY
D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D005260:Female; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008297:Male; D008875:Middle Aged
D017493:Leukocyte Common Antigens