Identification of Phoenix dactylifera L. varieties based on amplified fragment length polymorphism (AFLP) markers.
The amplified fragment length polymorphism (AFLP) technique was applied to identify palm varieties. Fluorescence labelled primers were used in selective amplifications and the amplified fragments were detected on capillary gel electrophoresis using an automated DNA sequencer with the analysis fragment option. This is a rapid and efficient technique for detecting a large number of DNA markers on the date palm. Phoenix dactylifera L. varieties Bou-Fegous, Medjool, and E-528 from Estación Phoenix (Elche), Spain, were analysed, yielding a total of 310 AFLP fragments derived from five primer combinations. The process for regenerating the date palm cultivars from in vitro tissue culture should yield individuals phenotypically and genetically identical to the explant they are derived from. The AFLP markers obtained were successfully used for comparing and identifying vitroplants of palm.
Diaz
Susana
S
Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, 03080 Alicante, Spain.
Pire
Carmen
C
Ferrer
Juan
J
Bonete
Maria José
MJ
D028023:Arecaceae / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes / Q000737:chemistry; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D018745:Genome, Plant; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005456:Fluorescent Dyes; D005819:Genetic Markers