title: Genetic differentiation of Aedes aegypti (Diptera: Culicidae), the major dengue vector in Brazil.
abstract: In 2000, Brazil reported 180,137 cases of dengue, approximately 80% of the total in the Americas. However, little is known about gene flow among the vector populations in Brazil. Random amplified polymorphic DNA (RAPD) was used to study the genetic structure of Aedes aegypti in 15 populations from five states, with a range extending 2,800 km. An analysis of 47 polymorphic RAPD loci estimated gene flow at the macro- (different states) and micro- (different cities) geographical levels. Genetic polymorphism was high (H(S) = 0.274), and high levels of genetic differentiation existed both between different states (G(ST) = 0.317) and between cities or neighborhoods in each state (G(ST) = 0.085-0.265). These values are higher than those described for any other populations of A. aegypti.
authors.affiliation: Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife-PE, Brazil. tans@cpqam.fiocruz.br
authors.forename: C F J
authors.initials: CF
authors.lastname: Ayres
authors.forename: M A V
authors.initials: MA
authors.lastname: Melo-Santos
authors.forename: A M
authors.initials: AM
authors.lastname: Solé-Cava
authors.forename: A F
authors.initials: AF
authors.lastname: Furtado
mesh_terms: D000330:Aedes / Q000145:classification / Q000235:genetics* / Q000821:virology; D000818:Animals; D001938:Brazil; D003715:Dengue / Q000635:transmission*; D003716:Dengue Virus / Q000302:isolation & purification*; D014644:Genetic Variation; D005843:Geography; D006801:Humans; D007303:Insect Vectors / Q000235:genetics* / Q000821:virology; D010802:Phylogeny; D014329:Tropical Climate