title: [Changes in bronchoalveolar lavage fluid of rats exposed to metal grinding dusts].
abstract: OBJECTIVE
  To investigate the effects of composite grinding dusts on rat respiratory system.
  
  
  METHODS
  Rats were administrated with grinding dusts by intratracheal injection. After 2 weeks, the total numbers of cells, the percentage of differential cell, the survival rate of cell, and the activity of lactate dehydrogenase(LDH) and alkaline phosphatase(ALP) in bronchoalveolar lavage fluid were analyzed.
  
  
  RESULTS
  Along with increasing concentration of grinding dusts, the total number of cells in lavage also increased, and was more than that in quartz group. Compared with control group, the percentage of neutrophil in lavage of rats treated with grinding dust and quartz significantly increased and meanwhile that of macrophage significantly decreased[PMN: quartz group (33.83 +/- 4.54)%; grinding dusts group (26.50 +/- 3.99)%, (36.00 +/- 3.58)%, (38.00 +/- 2.10)% at 10, 25, 50 mg/ml respectively. Macrophages: quartz group (62.17 +/- 4.54)%; grinding dusts group (70.83 +/- 3.66)%, (60.83 +/- 2.14)%, (58.17 +/- 2.48)%] while those in control group were (2.83 +/- 0.75)%, (95.67 +/- 1.21)% respectively. The cell survival rate in lavage in control group was 80%, but that in grinding dust and TiO2 group significantly decreased(P < 0.01). The activity of LDH and ALP in all rats treated with dusts obviously increased, and there was significant difference compared with control group(P < 0.01 or P < 0.05). Furthermore, there was significant difference between grinding dust group and quartz group, and between grinding dust group and TiO2 group respectively.
  
  
  CONCLUSIONS
  Metal grinding dust is very harmful to rat's lung cells and may cause fibrogenesis in the lungs.
authors.affiliation: Department of Occupational Health, School of Public Health, Harbin Medical University, Harbin 150001, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Wu
authors.forename: Lianming
authors.initials: L
authors.lastname: Wang
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Huiming
authors.initials: H
authors.lastname: Sun
authors.forename: E J
authors.initials: EJ
authors.lastname: Love
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004391:Dust; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000473:pathology*; D008670:Metals; D051381:Rats
chemical_list: D004391:Dust; D008670:Metals; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase