title: Resonance light scattering spectroscopy study of interaction between gold colloid and thiamazole and its analytical application.
abstract: In this paper, we used resonance light scattering (RLS) spectroscopy to study the interaction between thiol-containing pharmaceutical-thiamazole and gold colloid. At pH 5.2, the resonance light scattering spectrum of gold nanoparticles has a maximum peak at 555 nm and the RLS intensity is enhanced by trace amount of thiamazole due to the interaction between thiamazole and gold colloid. The binding of colloidal gold to thiamazole results in ligand-induced aggregation of colloidal gold, which was characterized by RLS spectrum, ultraviolet-visible (UV-Vis) spectrum, and transmission electron microscopy (TEM). Based upon the study, we proposed a highly sensitive, gold colloid-based assay using RLS spectrum to detect pharmaceuticals for the first time. The mechanism of binding interaction between Au colloid and thiamazole was also discussed.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.forename: Daiwen
authors.initials: D
authors.lastname: Pang
authors.forename: Ruxiu
authors.initials: R
authors.lastname: Cai
mesh_terms: D000042:Absorption; D006046:Gold; D017979:Gold Colloid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008713:Methimazole / Q000737:chemistry*; D008854:Microscopy, Electron; D008956:Models, Chemical; D036103:Nanotechnology; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D017979:Gold Colloid; D008713:Methimazole; D006046:Gold