Relationship between Candida albicans virulence during experimental hematogenously disseminated infection and endothelial cell damage in vitro.
Candida albicans must penetrate the endothelial cell lining of the vasculature to invade the deep tissues during a hematogenously disseminated infection. We compared 27 C. albicans mutants with their wild-type parent for their capacity to damage endothelial cells in vitro and cause a lethal infection in mice following tail vein inoculation. Of 10 mutants with significantly impaired capacity to damage endothelial cells, all had attenuated virulence. Therefore, the endothelial cell damage assay can be used as a screen to identify some virulence factors relevant to hematogenously disseminated candidiasis.
Sanchez
Angela A
AA
St. Johns Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502, USA.
Johnston
Douglas A
DA
Myers
Carter
C
Edwards
John E
JE
Mitchell
Aaron P
AP
Filler
Scott G
SG
D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D003601:Cytotoxicity Tests, Immunologic; D042783:Endothelial Cells / Q000382:microbiology* / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D014470:Umbilical Cord; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
D037521:Virulence Factors