A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product.
Nagy
Zsolt B
ZB
Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary.
Felföldi
Ferenc
F
Tamás
László
L
Puskás
László G
LG
D004252:DNA Mutational Analysis; D004262:DNA Restriction Enzymes; D008722:Methods; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D004262:DNA Restriction Enzymes