title: Effect of resistance training on Na,K pump and Na+/H+ exchange protein densities in muscle from control and patients with type 2 diabetes.
abstract: Ten patients with type 2 diabetes and seven controls were strength-trained with one leg for 30 min three times per week for 6 weeks. The training-induced changes in the protein densities of the Na,K-pump subunits and the Na+/H+ exchanger protein NHE1 were quantified with Western blotting of needle biopsy material obtained from trained and untrained legs of both groups. Training increased the bench press and knee-extensor force by 77+/-15 and 28+/-1%, respectively, in the control subjects, and by 75+/-7 and 42+/-8%, respectively, in the diabetics. In the control subjects the Na,K-pump isoform alpha1 was increased by 37% (P<0.05) in trained compared to untrained leg, and in the diabetics the alpha1 content was 45% higher (P=0.052) in trained compared to untrained leg. For the alpha2 isoform the corresponding values were 21% and 41% (P<0.05), respectively. The content of the beta1 subunit in the control subjects was 33% higher (P<0.05) in trained compared to untrained leg, and 47% higher (P=0.06) in trained compared to untrained leg in the diabetics. Thus, a limited amount of strength-training is able to increase the Na,K-pump subunit and isoform content both in controls and in patients with type 2 diabetes.
authors.affiliation: Department of Medical Physiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Flemming
authors.initials: F
authors.lastname: Dela
authors.forename: Mads
authors.initials: M
authors.lastname: Holten
authors.forename: Carsten
authors.initials: C
authors.lastname: Juel
mesh_terms: D000704:Analysis of Variance; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis* / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis* / Q000235:genetics; D014891:Weight Lifting / Q000502:physiology*
chemical_list: D017923:Sodium-Hydrogen Exchangers; D000254:Sodium-Potassium-Exchanging ATPase