Preparation and characterization of collagen-hydroxyapatite composite used for bone tissue engineering scaffold.
In this study, highly porous collagen-HA scaffolds were prepared by solid-liquid phase separation method. Microstructure of the composites was characterized by SEM, TEM and XRD. The results show that collagen-HA scaffolds are porous with three-dimension interconnected fiber microstructure, pore sizes are 50-150 microm, and HA particles are dispersed evenly among collagen fiber. Compared with pure collagen, the mechanical property of collagen-HA composite improves significantly. To gain further insight into cell growth throughout 3D scaffolds, the cell proliferation and attachment on the scaffold in vitro was investigated. The collagen-HA composite has good biocompatibility, and adding HA does not affect the histocompatibility of the scaffold materials. The porous collagen-HA composite is suitable as scaffold used for bone tissue engineering.
Liu
Lingrong
L
Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, PR China. liulingrong@vip.sina.com
Zhang
Lihai
L
Ren
Baizhi
B
Wang
Fujun
F
Zhang
Qiqing
Q
D000818:Animals; D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division; D003094:Collagen; D003188:Composite Resins / Q000138:chemical synthesis* / Q000494:pharmacology; D006882:Hydroxyapatites; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008854:Microscopy, Electron; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D003188:Composite Resins; D006882:Hydroxyapatites; D003094:Collagen