title: Differential expression of T-bet, a T-box transcription factor required for Th1 T-cell development, in peripheral T-cell lymphomas.
abstract: We studied T-bet expression in 91 cases of peripheral T-cell lymphoma (PTCL) by immunostaining and found expression in 42 cases (46%), including all 5 lymphoepithelioid lymphoma cases and 12 (86%) of 14 angioimmunoblastic lymphoma cases, but only 9 (25%) of 36 anaplastic large cell lymphoma cases. Expression of T-bet in PTCL correlates with expression of other markers of Th1 T-cell differentiation, including CXCR3 (P < .0001), CD69 (P = .0013), LEF-1 (P = .0007), and OX40/CD134 (P = .005), and absence of expression of markers of Th2 T-cell differentiation, including CD30 (P = .0001) and CXCR4 (P = .0144). Of 22 cases of PTCL immunoreactive for all Th1-associated markers previously studied and nonreactive for Th2-associated markers, 20 (91%) were immunoreactive for T-bet. Of 22 PTCL cases immunoreactive for Th2-associated markers studied and nonreactive for all Th1-associated markers studied, 4 (18%) were immunoreactive for T-bet. The remaining 47 PTCL cases (52%) exhibited incomplete or mixed staining for Th1- and Th2-associated markers, with 18 (38%) of 47 immunoreactive for T-bet. T-bet is a new marker that may contribute to the diagnosis and subtyping of PTCLs. T-bet expression in these neoplasms provides further support for a model of PTCL in which tumor subsets express markers of, and may be derived from, Th1- or Th2-committed T cells.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
authors.forename: Peter
authors.initials: P
authors.lastname: van den Elzen
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Weng
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Shahsafaei
authors.forename: Laurie H
authors.initials: LH
authors.lastname: Glimcher
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000737:chemistry; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000378:metabolism*; D008232:Lymphoproliferative Disorders / Q000276:immunology / Q000378:metabolism; D020825:T-Box Domain Proteins; D018417:Th1 Cells / Q000502:physiology*; D018418:Th2 Cells / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis*; D000098815:T-bet Transcription Factor
chemical_list: D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors