Stimulation of midgut stem cell proliferation by Manduca sexta alpha-arylphorin.
Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the observed stem cell proliferation. SDS-PAGE analysis of the protein results in a single band of 77 kDa. Sequences of tryptic peptides from this protein are identical to internal sequences of the storage hexamer alpha-arylphorin. The alpha-arylphorin isolated by our procedure represents a small fraction of the total arylphorin present in the fat body extract. However, it alone seems responsible for the stimulation of mitotic activity in H. virescens midgut stem cells.
Blackburn
Michael B
MB
Insect Biocontrol Laboratory, Plant Sciences Institute, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA. blackbm@ba.ars.usda.gov
Loeb
Marcia J
MJ
Clark
Edward
E
Jaffe
Howard
H
D000595:Amino Acid Sequence; D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004064:Digestive System / Q000166:cytology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D005216:Fat Body / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D018402:Manduca / Q000166:cytology* / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D006023:Glycoproteins; D019476:Insect Proteins; D010446:Peptide Fragments; C041137:storage proteins, Insecta