Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER.
A family of five-pass transmembrane proteins (FinGERs) were identified from the protein sequence database. The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species. Eight family members were found in human. Multiple sequence alignment revealed three regions conserved among all family members. All of the human family members were expressed widely in various tissues. The human proteins were localized in and around the Golgi apparatus and may also be in the ER to some extent. The Golgi apparatus was fragmented by overexpression of the five of the family members. Some of the members were found to interact by yeast two-hybrid analysis, suggesting the formation of a complex. These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus.
Shakoori
Abbas
A
Division of Molecular Membrane Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan.
Fujii
Gourou
G
Yoshimura
Shin-Ichiro
S
Kitamura
Masashi
M
Nakayama
Kazuhisa
K
Ito
Takashi
T
Ohno
Hiroshi
H
Nakamura
Nobuhiro
N
D000595:Amino Acid Sequence; D002352:Carrier Proteins; D030562:Databases, Protein; D004721:Endoplasmic Reticulum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D021381:Protein Transport; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins