Characterization of a beta-D-(1-->3)-glucan from the marine diatom Chaetoceros mülleri by high-resolution magic-angle spinning NMR spectroscopy on whole algal cells.
High-resolution magic-angle spinning (hr-MAS) NMR spectroscopy was used to record NMR spectra of a cell paste from the marine diatom Chaetoceros mülleri. This gave information on a cellular storage polysaccharide identified as a beta-D-(1-->3)-linked glucan, using hr-MAS one-dimensional 1H and 13C, two-dimensional 1H,1H-COSY and 13C,1H-correlation spectroscopy. The same structural information was deduced from the liquid state NMR data on the glucan extracted from C. mülleri. The extracted glucan proved to be a beta-D-(1-->3)-linked glucan with a degree of polymerization of 19 and a degree of beta-D-(1-->6) branching of 0.005. The hr-MAS spectrum of the diatom showed several nonglucan resonances in the carbohydrate region of the NMR spectrum (60-103 ppm) that were shown to be noncarbohydrate resonances by means of two-dimensional 13C,1H- and 1H,1H-correlated NMR data.
Størseth
Trond R
TR
Department of Bioresources, SINTEF Fisheries and Aquaculture, 7465, Trondheim, Norway. trond.r.storseth@sintef.no
Hansen
Karina
K
Skjermo
Jorunn
J
Krane
Jostein
J
D002236:Carbohydrate Conformation; D017377:Diatoms / Q000737:chemistry* / Q000166:cytology*; D005936:Glucans / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D005936:Glucans