title: Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling.
abstract: Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/ml range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.
authors.forename: Weiping
authors.initials: W
authors.lastname: Shao
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Isabelle
authors.initials: I
authors.lastname: Laroche
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Zong
authors.forename: Dhavalkumar D.
authors.initials: DD
authors.lastname: Patel
authors.forename: Stephen
authors.initials: S
authors.lastname: Kingsmore
authors.forename: Steven P.
authors.initials: SP
authors.lastname: Piccoli