[Study on DNA methylation status of WT1 gene promoter in leukemia cell].
OBJECTIVE
To analyse the WT1 expression and its DNA methylation status of its promoter domain.


METHODS
The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.


RESULTS
WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.


CONCLUSIONS
DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.
Wang
Quan-shun
QS
Department of Hematology, PLA General Hospital, Beijing 100853, China.
Yu
Li
L
Zhao
Yu
Y
Han
Wei-dong
WD
Gao
Chun-ji
CJ
Lou
Fang-ding
FD
D045744:Cell Line, Tumor; D019175:DNA Methylation; D016162:Genes, Wilms Tumor; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic