Experimental evidence for the physiological role of bacterial luciferase in the protection of cells against oxidative stress.
The origin and function of bioluminescence was considered a problematic question of the Charles Darwin theory. Early evolution of bacterial luminescence and its current physiological importance seem to be especially mysterious. Recently, it was proposed that stimulation of DNA repair may be a physiological role for production of light by bacterial cells. On the other hand, it was also proposed that primary role of luminescent systems could be detoxification of the deleterious oxygen derivatives. Although some previous results might suggest that this hypothesis can be correct, until now experimental evidence for such a mechanism operating in bacterial cells and having physiological importance was generally lacking. Here we demonstrate that in the presence of various oxidants (hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and ferrous ions) at certain concentrations in the culture medium, growth of Vibrio harveyi mutants luxA and luxB, but not of the mutant luxD, is severely impaired relative to wild-type bacteria. This deleterious effect of oxidants on the mutants luxA and luxB could be significantly reduced by addition of the antioxidants A-TEMPO or 40H-TEMPO. We conclude that bacterial luciferase may indeed play a physiological role in the protection of cells against oxidative stress.
Szpilewska
Hanna
H
Institute of Oceanology, Polish Academy of Sciences, Sw. Wojciecha 5, 81-347 Gdynia, Poland.
Czyz
Agata
A
Wegrzyn
Grzegorz
G
D000975:Antioxidants / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001555:Benzene Derivatives / Q000378:metabolism / Q000633:toxicity; D003497:Cyclic N-Oxides / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism / Q000633:toxicity; D005798:Genes, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D008156:Luciferases / Q000502:physiology*; D008163:Luminescent Measurements; D016254:Mutagenesis, Insertional; D020125:Mutation, Missense; D016877:Oxidants / Q000378:metabolism / Q000633:toxicity; D018384:Oxidative Stress / Q000502:physiology*; D014733:Vibrio / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D020122:tert-Butylhydroperoxide / Q000378:metabolism / Q000633:toxicity
D000975:Antioxidants; D001426:Bacterial Proteins; D001555:Benzene Derivatives; D003497:Cyclic N-Oxides; D005296:Ferrous Compounds; D016877:Oxidants; C015527:4-amino-TEMPO; C020748:ferrous sulfate; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D008156:Luciferases; C071832:alkanol monooxygenase (FMN-linked); C007164:cumene hydroperoxide