Dysregulation of IL-10 and IL-12p40 in secondary progressive multiple sclerosis.
Multiple sclerosis (MS) is a putative T helper 1 (Th-1) mediated inflammatory disorder of the central nervous system, and levels of pro-inflammatory and anti-inflammatory cytokines have been found to correlate with changes in MS relapses. However, it is unclear if cytokine profiles differ between relapsing-remitting (RRMS) versus secondary progressive (SPMS) disease stages. Cytokine production (IL-2, IL-4, IL-5, IL-10, IL-12p40, TNF-alpha, and IFN-gamma) was assessed by cytometric bead array (CBA) and intracellular cytokine staining from alphaCD3 antibody and mitogen stimulated peripheral blood mononuclear cells (PBMCs) from female RRMS and SPMS patients, and healthy controls. Significantly increased production of IL-12p40 and decreased production of IL-10 were observed in SPMS patients. Differences in immune responsiveness in RRMS and SPMS are important in the understanding of the evolution of the immunopathogenesis of the disease and for the development of disease type specific treatments.
Soldan
Samantha S
SS
Department of Neurology, Reed Neurological Research Center, University of California School of Medicine, 750 Westwood Plaza, Los Angeles, CA 90095, USA.
Alvarez Retuerto
Ana Isabel
AI
Sicotte
Nancy L
NL
Voskuhl
Rhonda R
RR
D000328:Adult; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D053762:Interleukin-12 Subunit p40; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D020528:Multiple Sclerosis, Chronic Progressive / Q000276:immunology* / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D012044:Regression Analysis
D053762:Interleukin-12 Subunit p40; D021122:Protein Subunits; D016753:Interleukin-10; D018664:Interleukin-12