Common single nucleotide polymorphisms of the MDR1 gene have no influence on its mRNA expression level of normal kidney cortex and renal cell carcinoma in Japanese nephrectomized patients.
In this study, we have quantified the mRNA expression levels of multidrug resistance gene 1 (MDR1) in the normal kidney cortex and renal cell carcinoma (RCC) segments from 24 Japanese nephrectomized patients by real-time polymerase chain reaction (PCR). The mRNA expression level of MDR1 in RCC segments was significantly decreased in comparison with each normal segment (P=0.0042, by Student's paired t-test). In addition, the ten common single nucleotide polymorphisms (SNPs) of the MDR1 gene in the patients were assessed using the PCR-restriction enzyme fragment length polymorphism method to investigate the influence of these SNPs on its mRNA expression levels. The allele frequencies of these SNPs were comparable with our previous report in the Japanese recipients of living-donor liver transplantation (Goto et al., Pharmacogenetics 12:451-457; 2002). MDR1 expression levels in the normal kidney cortex were independent on the five SNPs, which were polymorphic in the Japanese population. Furthermore, the effect of the SNPs on expression levels of MDR1 mRNA in RCC segments was not recognized. These findings suggest that the common SNPs in the MDR1 gene have no influence on the expression of its transcript in RCC segments as well as in the normal kidney cortex.
Uwai
Yuichi
Y
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Masuda
Satohiro
S
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Goto
Maki
M
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Motohashi
Hideyuki
H
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Saito
Hideyuki
H
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Okuda
Masahiro
M
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan.
Nakamura
Eijirou
E
Department of Urology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ito
Noriyuki
N
Department of Urology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ogawa
Osamu
O
Department of Urology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Inui
Ken-Ichi
KI
Department of Pharmacy, Kyoto University Hospital, Sakyo-ku, Kyoto 606-8507, Japan. inui@kuhp.kyoto-u.ac.jp.
D000328:Adult; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D005453:Fluorescence; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D007564:Japan; D007672:Kidney Cortex / Q000378:metabolism*; D007680:Kidney Neoplasms; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger