title: Protective effect of shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells.
abstract: The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.
authors.affiliation: Department of Oriental Rehabilitation Medicine, College of Oriental Medicine Kyung Hee University, Seoul, Korea.
authors.forename: Hyun-Taeg
authors.initials: HT
authors.lastname: Shin
authors.forename: Seok-Hee
authors.initials: SH
authors.lastname: Chung
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Hyun-Dae
authors.initials: HD
authors.lastname: Shin
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Geon-Ho
authors.initials: GH
authors.lastname: Bahn
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Paik
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Park
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D006861:Hydrogen Peroxide; D009474:Neurons / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D013997:Time Factors
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D016194:Receptors, N-Methyl-D-Aspartate; C484999:shenqui-wan; D006861:Hydrogen Peroxide