Expression of SR-BI receptor and StAR protein in rat ocular tissues.
The class-B type-I scavenger receptor (SR-BI) plays a key role in cholesterol homeostasis; it mediates the selective uptake of lipoprotein cholesterol to steroidogenic tissues. We show by RT-PCR, western blot, in situ hybridization and immunohistochemistry analysis that SR-BI is highly expressed in different neuro-retinal and non-neuronal cells types on rat eye. Immunohistochemistry of the steroidogenic acute regulatory protein (StAR) involved in neurosteroid production showed the same expression pattern than SR-BI in rat eye. Our results may suggest a key role of these genes in the ocular cholesterol metabolism for membranes biosynthesis and neurosteroidogenesis.
Provost
Alexandra C
AC
Centre de recherche thérapeutique en ophtalmologie (CERTO), équipe d'accueil no 2502 du ministère de l'Enseignement supérieur, université René-Descartes, faculté de médecine Necker-Enfants Malades, 156, rue de Vaugirard 75015 Paris, France.
Péquignot
Marie O
MO
Sainton
Karine M
KM
Gadin
Stéphanie
S
Sallé
Sandrine
S
Marchant
Dominique
D
Hales
Dale B
DB
Abitbol
Marc
M
D000818:Animals; D001921:Brain / Q000502:physiology; D018955:CD36 Antigens; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D009799:Ocular Physiological Phenomena; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains; D011971:Receptors, Immunologic / Q000235:genetics*; D051116:Receptors, Scavenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B; D014158:Transcription, Genetic; D000098682:Steroidogenic Acute Regulatory Protein
D018955:CD36 Antigens; D010750:Phosphoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; C495125:Scarb1 protein, rat; D051122:Scavenger Receptors, Class B; D000098682:Steroidogenic Acute Regulatory Protein