Detection of specific noncovalent interaction of peptide with DNA by MALDI-TOF.
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was used to obtain spectra of peptide-DNA complexes formed by basic domain (BD15) of c-Fos protein and DNA AP-1 site (5'-TGAGTCA-3'). The noncovalent interaction between single stranded DNA and BD15 was observed and confirmed to be an ionic one between the negatively charged sugar-phosphate backbone of DNA and positively charged side chains of Arg- and lys-rich peptides as demonstrated by Vertes and coworkers and Woods and coworkers. But the specific noncovalent interaction between DNA AP-1 site and the dimer of BD15 was firstly detected in this paper. Various different sequence DNAs were studied and it was found that this interaction is a sequence-specific one, and AP-1 site was essential for this interaction. This specific interaction depends on the matrix. It was only observed in the ATT matrix and not in the other two matrixes (CHCA and DHBA).
Luo
Shi-Zhong
SZ
The Key Laboratory for Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, China.
Li
Yan-Mei
YM
Qiang
Wei
W
Zhao
Yu-Fen
YF
Abe
Hiroshi
H
Nemoto
Tadashi
T
Qin
Xu-Rong
XR
Nakanishi
Hiroshi
H
D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018808:Transcription Factor AP-1 / Q000737:chemistry / Q000378:metabolism
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1