[Relation of genetic polymorphism of NQO1 and GSTT1 with risks of chronic benzene poisoning].
OBJECTIVE
To explore the relation between genetic polymorphisms of NQO1, GSTT1 and risks of chronic benzene poisoning (BP).


METHODS
A case-control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated. Polymerase chain reaction (PCR), denaturing high-performance liquid chromatography(DHPLC) and sequencing were used to detect the single nucleotide polymorphisms(SNPs) of the promoter and complete coding-region of NQO1 gene. Multiple PCR was used to detect GSTT1 genotype.


RESULTS
In smoking population, there was 7.73-fold (95% CI: 1.71-34.97, P = 0.010) of risk in BP subjects carrying NQO1c. 609 T/T genotype, compared with those carrying C/C and C/T. genotype. In drinking population, the individuals carrying the 6th extron of NQO1c. 609 T/T homozygote genotype had a 11.00-fold(95% CI: 1.89-63.83, P = 0.005) risk of BP compared to those with NQO1c. 609 C/T and C/C genotypes.


CONCLUSIONS
The subjects carrying NQO1c. 609 T/T genotype and together with the habit of smoking or drinking may be more susceptible to BP.
Wan
Junxiang
J
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Shi
Jinxiu
J
Guan
Jiru
J
Ye
Rong
R
Gao
Xiaoling
X
Liu
Weiwei
W
Hui
Lijian
L
Cao
Duozhi
D
Jin
Xipeng
X
Hu
Gengxi
G
Xia
Zhaolin
Z
D001554:Benzene / Q000506:poisoning*; D016022:Case-Control Studies; D000431:Ethanol / Q000009:adverse effects; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D009784:Occupational Diseases / Q000235:genetics*; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D012907:Smoking / Q000009:adverse effects
D000431:Ethanol; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase; D001554:Benzene