title: Mitochondrial DNA diversity of Pakistani goats.
abstract: Pakistan contains numerous domestic goat breeds, but until now there has been no comprehensive study on genetic diversity or a phylogenetic analysis of Pakistani goats. In this study, we analysed the complete mitochondrial DNA D-loop and the cytochrome b gene of 13 Pakistani domestic goat breeds (Capra hircus) and one wild goat, the Sindh Ibex (Capra aegagrus blythi). The phylogenetic analyses and sequence divergence (SD) established four distinct mt-lineages termed as A, B and C (previously reported) and a new lineage D. The Sindh Ibex appeared as an outgroup of domestic goats. The estimated divergence times between the most recently evolved mt-lineages A and D were from 260,483 to 371,052 YA. This suggested that at least four different strains of wild Capra might have been the source of the modern domestic goats. The new mt-lineage D revealed high SD from mt-lineage A and may be the oldest branch under domestication, while mt-lineages B and C showed lower SD and might have been domesticated during an advanced stage of the domestication process.
authors.affiliation: Graduate School of Science and Technology, Kobe University, Kobe, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sultana
authors.forename: H
authors.initials: H
authors.lastname: Mannen
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005843:Geography; D006041:Goats / Q000145:classification / Q000235:genetics*; D010154:Pakistan; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial