Mapping of ESTs derived from pre-implantation stage cattle embryos to allocate 16 new additions to the ordered comparative map of cattle and human.
Twenty expressed sequence tags (ESTs) derived from cDNA libraries of different developmental stages of embryos were mapped using a whole genome bovine hamster radiation hybrid panel. These include 14 markers representing genes, most of which have not so far been mapped in cattle, with another three being novel in both cattle and human. The markers were placed on specific chromosomes with high LOD scores and except two all localizations fit the current human and cattle comparative map. The assignment of these genes further enriches the cattle genome map and also contributes to the international effort of generating comparative maps.
Mamo
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Institute of Animal Breeding Sciences, University of Bonn, Bonn, Germany. lilykacha@hotmail.com
Wimmers
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Gilles
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Kata
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SR
Schellander
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K
Ponsuksili
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D000818:Animals; D019295:Computational Biology; D003433:Crosses, Genetic; D020224:Expressed Sequence Tags; D007059:Ictaluridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*