Advances in high-throughput screening: biomolecular interaction monitoring in real-time with colloidal metal nanoparticles.
The post-genomic era is revolutionizing the drug discovery process. The new challenges in the identification of therapeutic targets require efficient technological tools in order to be properly addressed. Label-free detection systems use proteins or ligands coupled to materials of which the physical properties are measurably modified upon specific interactions. Among the label-free systems currently available, the use of metal nanocolloids offers enhanced throughput and flexibility for real-time biomolecular recognition monitoring at a reasonable cost.
Englebienne
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Biocybernetics Unit, Laboratory for Experimental Medicine, and Department of Nuclear Medicine, Free University of Brussels, Brugmann Hospital, Place Van Gehuchten 4, B-1020 Brussels, Belgium. penglebi@ulb.ac.be
Van Hoonacker
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Verhas
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Khlebtsov
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D015374:Biosensing Techniques / Q000379:methods*; D003102:Colloids / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D019216:Metals, Heavy / Q000737:chemistry*; D036103:Nanotechnology; D020349:Surface Plasmon Resonance
D003102:Colloids; D008024:Ligands; D019216:Metals, Heavy