Stable transplantation results of magnetically retracted islets: a novel method.
OBJECTIVE
Large quantities of pure viable donor islets are necessary for clinical transplantation. At present, low yields and low viability of pancreatic islets after transplantation necessitate the use of multiple donors for a single recipient. In this study an improved method for obtaining large quantities of pure viable islets of Langerhans for transplantation was developed in the rat.


METHODS
Islets of Langerhans were isolated from Albino Oxford rats. The donor pancreata were perfused in situ with iron oxide, which resulted in entrapment of iron particles in the capillaries of the islets. Subsequently, the islets were isolated by magnetic retraction. Islets obtained with this method were compared with islets obtained by density gradient-isolated islets with respect to yields, purity, and insulin production capacity. Islets isolated with the magnetic retraction method were transplanted under the renal capsule of streptozotocin-induced diabetic recipients. Blood-glucose levels in the recipients were monitored for 2 months after transplantation.


RESULTS
This method yielded more pure and viable islets than the conventional protocol. No contamination of exocrine tissue was observed after isolation. Furthermore, the islets isolated by magnetic retraction stained strongly positive for insulin during the entire observation period in vitro, and produced high amounts of insulin upon a challenge with glucose. The islets that were obtained by this new protocol were suitable for safe and effective transplantation.


CONCLUSIONS
We have shown that both the quantity and quality of islets obtained with this method were sufficient to induce insulin independence in a diabetic recipient using islets from only one donor.
Pinkse
G G M
GG
Deptartment of Pathology, Leiden University Medical Center, Building 1, L1-Q, PO Box 9600, 2300 RC Leiden, The Netherlands. G.Pinkse@lumc.nl
Steenvoorde
E
E
Hogendoorn
S
S
Noteborn
M
M
Terpstra
O T
OT
Bruijn
J A
JA
De Heer
E
E
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005947:Glucose / Q000494:pharmacology; D018189:Immunomagnetic Separation / Q000379:methods*; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D005947:Glucose