Production of transgenic lily plants by Agrobacterium-mediated transformation.
A system for the production of transgenic plants was developed for the Oriental hybrid lily, Lilium cv. Acapulco, by Agrobacterium-mediated genetic transformation. Filament-derived calli were co-cultivated with A. tumefaciens strain EHA101/pIG121Hm, which harbored a binary vector carrying the neomycin phosphotransferase II, hygromycin phosphotransferase, and intron-containing beta-glucuronidase genes in the T-DNA region. Six hygromycin-resistant (Hyg(r)) culture lines were obtained from 200 calli by scratching them with sandpaper prior to inoculation and using NH(4)NO(3)-free medium for co-cultivation and a hygromycin-containing regeneration medium for selection. Hyg(r) culture lines regenerated shoots, which developed into plantlets following transfer to a plant growth regulator-free medium. All of these plantlets were verified to be transgenic by GUS histochemical assay and inverse PCR analysis.
Hoshi
Y
Y
Niigata Agricultural Research Institute, 857 Nagakura, 940-0826, Nagaoka, Niigata, Japan. yhoshi@ari.pref.niigata.jp
Kondo
M
M
Mori
S
S
Adachi
Y
Y
Nakano
M
M
Kobayashi
H
H
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D027762:Lilium / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic / Q000235:genetics*
D017931:DNA Primers; D005966:Glucuronidase