title: Risk factors and reproductive disorders associated with canine herpesvirus-1 (CHV-1).
abstract: Canine herpesvirus-1 (CHV-1) is presumed to be enzootic in the dog population and is associated with fertility disorders and neonatal mortality. In this study we screened for risk factors affecting CHV-1 antibody titers and investigated the association between antibody titers and reproductive disorders. Therefore, serum from 545 dogs used for reproduction was analysed with an ELISA. Using a forward stepwise procedure and retaining significant risk factors (P<0.05), best fitting multifactorial generalized linear model (glm) procedures were built for males and females. The effect of antibody titers on reproductive disorders was analysed with logistic regression analysis. The association between reproductive disorders and seroprevalence was analysed in chi-square analyses using contingency tables. In both sexes, kennel cough and breeding management were found to have an impact on the CHV-1 antibody titer. Also, the influence of kennel cough on the antibody titer was correlated to the hygienic status of the kennel. In females, age, kennel size and cycle stage had an effect on CHV-1 antibody titers. Furthermore, kennel size and hygiene were found to be correlated. In males, mating experience had an impact on CHV-1 antibody titers. An association was observed between serological status and a history of abortion in bitches. In conclusion, this study suggests CHV-1 antibody titers may be affected by many factors, both on an environmental and host level. Therefore, interpretation of the serological status requires precaution. Furthermore, oronasal and venereal transmission seem to play a role in the spreading of infection.
authors.affiliation: Department of Clinical Sciences, Section Small Animal Reproduction, College of Veterinary Medicine, Université de Liège, Boulevard de Colonster 20, B44, 4000 Liège, Belgium. vronsse@ulg.ac.be
authors.forename: Veerle
authors.initials: V
authors.lastname: Ronsse
authors.forename: John
authors.initials: J
authors.lastname: Verstegen
authors.forename: Karine
authors.initials: K
authors.lastname: Onclin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Farnir
authors.forename: Hervé
authors.initials: H
authors.lastname: Poulet
mesh_terms: D000034:Abortion, Veterinary / Q000821:virology; D000375:Aging; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001947:Breeding; D004283:Dog Diseases / Q000635:transmission / Q000821:virology*; D004285:Dogs; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications / Q000635:transmission / Q000662:veterinary*; D002184:Herpesvirus 1, Canid / Q000276:immunology; D006799:Housing, Animal; D016015:Logistic Models; D008297:Male; D010298:Parity; D012098:Reproduction; D012307:Risk Factors; D012727:Sex Characteristics
chemical_list: D000914:Antibodies, Viral