Demonstration of functional receptors for noradrenaline and adenosine-5'-triphosphate, but not for prostaglandin E2, in HT-29 human colon cancer cell line.
1. The aim of the current study was to investigate in HT-29 human colon cancer cell line, the existence of functional receptors for the signalling molecules, noradrenaline (NA), prostaglandin E2 (PGE2), and adenosine-5'-triphosphate (ATP). 2. We utilized microphysiometry, which monitors on-line extracellular acidification rate (ECAR) as a measure of cellular metabolic activity, and how this variable is altered by signalling molecules. 3. Challenge with NA (5.9 microM) resulted in an increase in ECAR by approximately 24% of basal. 4. PGE2 (0.0284, 0.284 and 2.84 microM) hardly affected ECAR. 5. ATP (100 microM) elicited a biphasic effect on ECAR (increase and decrease in ECAR by about 58 and 10% of basal, respectively). 6. HT-29 cells were shown to express COX-2 by immunocytochemistry. 7. These data suggest the presence of functional receptors for NA and ATP, but not for PGE2 in HT-29 human colon cancer cell line.
Nylund
G
G
Department of Surgery, Institute of Surgical Sciences, Sahlgrenska University Hospital, SE-413 45 Göteborg, Sweden.
Nordgren
S
S
Delbro
D S
DS
D000255:Adenosine Triphosphate / Q000494:pharmacology; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins; D009638:Norepinephrine / Q000378:metabolism* / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000378:metabolism*; D018078:Receptors, Prostaglandin E / Q000187:drug effects / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism*
D007527:Isoenzymes; D008024:Ligands; D008565:Membrane Proteins; D011941:Receptors, Adrenergic; D018078:Receptors, Prostaglandin E; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D009638:Norepinephrine