title: The adrenal secretory serine protease AsP is a short secretory isoform of the transmembrane airway trypsin-like protease.
abstract: To further elucidate the role of proteases capable of cleaving N-terminal proopiomelanocortin (N-POMC)-derived peptides, we have cloned two cDNAs encoding isoforms of the airway trypsin-like protease (AT) from mouse (MAT) and rat (RAT), respectively. The open reading frames comprise 417 amino acids (aa) and 279 aa. The mouse AT gene was located at chromosome 5E1 and contains 10 exons. The longer isoform, which we designated MAT1 and RAT1, has a simple type II transmembrane protein structure, consisting of a short cytoplasmic domain, a transmembrane domain, a SEA (63-kDa sea urchin sperm protein, enteropeptidase, agrin) module, and a serine protease domain. The human homolog of MAT1 and RAT1 is the human AT (HAT). The shorter isoform, designated MAT2 and RAT2, which contains an alternative N terminus, was formerly described in the rat as adrenal secretory serine protease (AsP) and has been shown to be involved in the processing of N-POMC-derived peptides. In contrast to the long isoform, neither MAT2 and RAT2 (AsP) contain a transmembrane domain nor a SEA domain but an N-terminal signal peptide to direct the enzyme to the secretory pathway. The C terminus, covering the catalytic triad, is identical in both isoforms. Immunohistochemically, MAT/RAT was predominantly expressed in tissues of the upper gastrointestinal and the respiratory tract-but also in the adrenal gland. Moreover, isoform-specific RT-PCR and quantitative PCR analysis revealed a complex expression pattern of the two isoforms with differences between mice and rats. These findings indicate a multifunctional role of these proteases beyond adrenal proliferation.
authors.affiliation: Endocrinology and Diabetes Unit, Department of Medicine, University of Wuerzburg, Germany. immoh@citrus.ucr.edu
authors.forename: Immo A
authors.initials: IA
authors.lastname: Hansen
authors.forename: Martin
authors.initials: M
authors.lastname: Fassnacht
authors.forename: Stefanie
authors.initials: S
authors.lastname: Hahner
authors.forename: Fabian
authors.initials: F
authors.lastname: Hammer
authors.forename: Markus
authors.initials: M
authors.lastname: Schammann
authors.forename: Susanne R
authors.initials: SR
authors.lastname: Meyer
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Bicknell
authors.forename: Bruno
authors.initials: B
authors.lastname: Allolio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D012697:Serine Endopeptidases; C431509:adrenal secretory protease; C483682:airway trypsin-like protease, mouse; C483683:airway trypsin-like protease, rat