title: Altered immunoglobulin isotype profile and anti-immature worm surface immunoglobulins in mice harboring a praziquantel-resistant Schistosoma mansoni isolate.
abstract: After placement in mice of PZQ-sensitive and -insensitive S. mansoni isolates obtained from villagers responding and not responding to PZQ, parasitological criteria reflecting their biological development and also the host anti-immature worm immunoglobulin isotypes were examined 8 and 10 weeks post infection. Hepatic granuloma diameter, hepatic histopathological changes and immunolocalization of IgG and IgM on the surface of PZQ-sensitive and -resistant worms were also examined 10 weeks post infection. Data showed that parasitological criteria were not significantly different between mice infected with the PZQ-sensitive and -insensitive S. mansoni isolates. As regards serum immunoglobulins, in mice infected with the PZQ-insensitive S. mansoni isolate, IgG and IgG1 were significantly (p<0.05) lower 8 and 10 weeks post infection, respectively (1.41+/-0.07 and 1.08+/-0.10 and 1.35+/-0.06 and 1.09+/-0.07) than in mice infected with the PZQ-sensitive S. mansoni isolate (1.73+/-0.15 and 1.38+/-0.10 and 1.73+/-0.17 and 1.54+0.21) after the same observation periods. IgM level was nearly the same while IgE was lower than that recorded in mice infected with the PZQ-sensitive S. mansoni isolate. IgG immunofluorescence was also lower (60%+/-6.78) on the surface of resistant worms than that of sensitive worms (66.6%+/-5.27); meanwhile, hepatic granuloma diameter was significantly larger (296.5+/-3.0 vs 283.6+/-4.0) in mice infected with the PZQ-insensitive S. mansoni isolate with higher percentage of intact eggs. Differences in the immunogenic make up of PZQ-sensitive and -insensitive S. mansoni isolates qualitatively and/or quantitatively favoring a certain Th cell subpopulation response could be the underlying reason for such differences recorded in the host immunoglobulin isotype response and also the egg-induced hepatic histopathological changes.
authors.affiliation: Department of Immunology, Theodor Bilharz Research Institute, Guiza, Egypt.
authors.forename: Soad
authors.initials: S
authors.lastname: Hanallah
authors.forename: Naglaa
authors.initials: N
authors.lastname: El-Lakkany
authors.forename: Soheir
authors.initials: S
authors.lastname: Mahmoud
authors.forename: Mona
authors.initials: M
authors.lastname: Mousa
authors.forename: Sanaa
authors.initials: S
authors.lastname: Botros
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D000909:Antibodies, Helminth / Q000032:analysis / Q000097:blood*; D004195:Disease Models, Animal; D004351:Drug Resistance; D006099:Granuloma / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000032:analysis / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D010270:Parasite Egg Count; D011223:Praziquantel / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification*; D012555:Schistosomiasis mansoni / Q000097:blood / Q000276:immunology* / Q000473:pathology
chemical_list: D000871:Anthelmintics; D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007073:Immunoglobulin E; D011223:Praziquantel