Altered tropism of recombinant bovine adenovirus type-3 expressing chimeric fiber.
Recombinant bovine adenovirus-3 (BAV-3) has been used as a gene delivery vector for vaccination of calves. However, its usefulness as a vector for non-bovine species is limited due to poor transduction efficiency. To develop BAV-3 based vector for non-bovine species, we determined the feasibility of making targeted BAV-3 vector by modifying its natural tropism. We constructed a chimeric virus, BAV600, in which the knob region of the BAV-3 fiber protein was replaced with that from human adenovirus type 5 (HAV-5). Unmodified BAV-3 vector (BAV304) was able to transduce and direct the expression of green fluorescent protein (GFP) in non-bovine cells, with low efficiency. In contrast, the transduction efficiency of BAV600 in these cells was increased by 3-67-fold. Although, expression of early and late genes was detected in non-human cells, no progeny virus (BAV600) was detected in these cells. Our results suggest that it is possible to develop BAV-3 vectors with tropism for non-bovine species.
Wu
Qiaohua
Q
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
Tikoo
Suresh Kumar
SK
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D017921:Mastadenovirus / Q000235:genetics* / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D014161:Transduction, Genetic; D014779:Virus Replication
D036022:Capsid Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C018315:hexon capsid protein, Adenovirus; D049452:Green Fluorescent Proteins