A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries.
Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.
Dame
Michael K
MK
Department of Pathology, University of Michigan Medical School, 4224 Medical Science I Building, 1301 Catherine Street, Ann Arbor, Michigan 48109-0602, USA. mdame@med.umich.edu
Yu
Xinwen
X
Garrido
Rosario
R
Bobrowski
Walter
W
McDuffie
J Eric
JE
Murphy
Hedwig S
HS
Albassam
Mudher
M
Varani
James
J
D000818:Animals; D002469:Cell Separation / Q000379:methods; D004396:Coloring Agents; D003331:Coronary Vessels / Q000166:cytology*; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D008858:Microscopy, Phase-Contrast / Q000379:methods; D009131:Muscle, Smooth, Vascular / Q000166:cytology*
D004396:Coloring Agents