Dithizone derivatives as sensitive water soluble chromogenic reagents for the ion chromatographic determination of inorganic and organo-mercury in aqueous matrices.
Water-soluble sulfonate and the novel carboxylate analogues of dithizone, combined with ion interaction chromatography on a Dionex Acclaim 120 C18 silica column (250 x 4.6 mm id) with an eluent consisting of 10 mM tetrabutylammonium bromide and 60:40 methanol:water, have been developed as highly sensitive chromogenic ligands for the quantitative isocratic determination of inorganic and organo-mercury compounds in aqueous matrices in under 12 min. Using an optimised post column reagent system containing 0.65 mM dye, 0.5% Triton X-100 and 50 mM sodium hydroxide, good linearity (0-7.5 mg L(-1) R2 > 0.999), reproducibility using peak area measurements (RSD 0.69-1.38%, n = 8), and limits of detection (4-12 microg L(-1)) were achieved for methyl mercury, inorganic mercury and phenyl mercury.
Shaw
Matthew J
MJ
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Private Bag 75, Hobart 7001, Tasmania, Australia.
Jones
Phil
P
Haddad
Paul R
PR
D002614:Chelating Agents; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D002863:Chromogenic Compounds; D004230:Dithizone; D008628:Mercury / Q000032:analysis*; D012995:Solubility
D002614:Chelating Agents; D002863:Chromogenic Compounds; D004230:Dithizone; D008628:Mercury