title: Investigation of metabolic changes in irradiated rat brain tissue by means of 1H NMR in vitro relaxation study.
abstract: The effect of irradiation on concentrations and relaxation behaviour of brain metabolites was studied by means of high-resolution 1H NMR in vitro. The studies were performed on rat brains irradiated with the doses of 20 Gy applied in fractions of 2 Gy. Standard procedures were used to obtain HClO4 extracts of rat brains. The 1H NMR studies of the extracts solutions in D2O were performed using a Varian Inova-300 NMR spectrometer. The integral intensities of the metabolite signals were found to change during the irradiation cycle and after it. These changes are accompanied by the variations in the T1 relaxation times. N-acetylaspartate, glycerophosphocholine, phosphocholine, choline, creatine and phosphocreatine, myoinositol and taurine were analysed as potential markers of irradiation injury.
authors.affiliation: Department of Medical Physics, Institute of Oncology, ul. Wybrzeze Armii Krajowej, 15 Str., 44101 Gliwice, Poland. mary@io.gliwice.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Sokół
authors.forename: Waldemar M
authors.initials: WM
authors.lastname: Przybyszewski
authors.forename: Bozena
authors.initials: B
authors.lastname: Matlas
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects*; D001923:Brain Chemistry; D003401:Creatine / Q000032:analysis / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011522:Protons; D011829:Radiation Dosage; D051381:Rats; D013654:Taurine / Q000032:analysis / Q000378:metabolism*; D014020:Tissue Extracts / Q000032:analysis* / Q000737:chemistry*
chemical_list: D011522:Protons; D014020:Tissue Extracts; D013654:Taurine; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine