title: Laser-assisted microdissection applied to frozen surgical pathologic specimens - methodological aspects on RT-PCR.
abstract: Laser-assisted microdissection has become a unique technique for an accurate gene-expression profiling analysis in human tissues. The introduction of this approach requires the development of a reliable, efficient, and reproducible procedure for tissue processing. We report a systematic evaluation of the different relevant steps required to obtain sufficient quantity and good quality RNA for reverse transcriptase-polymerase chain reaction when using frozen surgical pathologic tissues as starting material. We propose an optimized and very efficient method with respect to time and effort that can easily be put into practice in research laboratory as well as in any pathology laboratory.
authors.affiliation: Laboratory of Pathology, Erasmus University Hospital, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Saal
authors.forename: Anne
authors.initials: A
authors.lastname: Gustin
authors.forename: Katia
authors.initials: K
authors.lastname: Rombaut
authors.forename: Pilar
authors.initials: P
authors.lastname: Pelaez
authors.forename: Sandrine
authors.initials: S
authors.lastname: Rorive
authors.forename: Myriam
authors.initials: M
authors.lastname: Remmelink
authors.forename: Isabelle
authors.initials: I
authors.lastname: Salmon
mesh_terms: D003106:Colon / Q000473:pathology; D005629:Frozen Sections / Q000592:standards*; D006801:Humans; D053685:Laser Therapy / Q000379:methods; D008168:Lung / Q000473:pathology; D042282:Microdissection / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D012334:RNA, Neoplasm / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*; D013961:Thyroid Gland / Q000473:pathology
chemical_list: D012334:RNA, Neoplasm