Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from eastern Slovakia.
Ixodes ricinus ticks (20 males, 20 females and 20 nymphs) collected in Kosice, Slovakia were examined for the presence of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato (s.l.) by PCR. 38.3 % of the tested ticks carried single infection of B. burgdorferi s.l. and 8.3 % were infected with A. phagocytophilum. Double infection of both pathogens was detected in 5 % of tested ticks. These results indicate that both B. burgdorferi s.l. and A. phagocytophilum co-circulate in the enzootic sites of Eastern Slovakia and may cause co-infection in humans.
Derdakova
Marketa
M
Faculty of Medicine, P. J. Safarik University, Srobarova 2, 041 80 Kosice, Slovak Republic. monickah@pobox.sk
Halanova
Monika
M
Stanko
Michal
M
Stefancikova
Asteria
A
Cislakova
Lydia
L
Pet'ko
Branislav
B
D041081:Anaplasma phagocytophilum / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004197:Disease Reservoirs; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018154:Slovakia / Q000453:epidemiology
D017931:DNA Primers; D004269:DNA, Bacterial