Erythrocyte zinc and carbonic anhydrase levels in nondialyzed chronic kidney disease patients.
OBJECTIVE
The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in nondialyzed chronic kidney disease patients, and observe the relationship with acidosis, zinc, anemia, and iron supplementation.


METHODS
Erythrocyte CA concentrations were measured in nondialyzed patients (n = 38) using enzyme-linked immunosorbent assays (ELISA). The zinc concentration was determined by atomic absorption spectrophotometer.


CONCLUSIONS
The CA levels were observed to be increased in the patients. The mean erythrocyte zinc concentration was also high and the levels of zinc in plasma were baseline values. Correlation was found between CAI and erythrocytes Zn (r = 0.46; P = 0.003), but there was no correlation with ferritin or pH and bicarbonate. The CA levels did not change after iron supplementation, but the zinc erythrocyte levels were reduced. It is concluded that the CA erythrocyte concentration in CKD patients is increased but this cannot be explained by iron deficiency or acidosis.
Mafra
Denise
D
Laboratory of Minerals, Faculty of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil. dmafra@terra.com.br
Cozzolino
Silvia M F
SM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000740:Anemia / Q000097:blood; D002256:Carbonic Anhydrases / Q000097:blood*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology*; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood; D007674:Kidney Diseases / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013054:Spectrophotometry, Atomic / Q000379:methods; D015032:Zinc / Q000097:blood*
D007501:Iron; D002256:Carbonic Anhydrases; D015032:Zinc