Defining a serological correlate of protection in rabbits for a recombinant anthrax vaccine.
In these studies, a serological correlate of protection against anthrax was identified in New Zealand white (NZW) rabbits that had been given one or two injections of various amounts of recombinant protective antigen (rPA) combined with aluminum hydroxide adjuvant (Alhydrogel). Rabbits were subsequently challenged by the aerosol route with spores of the Ames isolate of Bacillus anthracis. Results suggested that the antibody response, as determined by the quantitative anti-rPA IgG ELISA and toxin neutralizing antibody (TNA) assay, were significant predictors (P<0.0015) of protection against a B. anthracis aerosol spore challenge in rabbits.
Little
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Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702-5011, USA. stephen.little@amedd.army.mil
Ivins
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Fellows
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Pitt
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Norris
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Andrews
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D000276:Adjuvants, Immunologic; D000336:Aerosols; D000536:Aluminum Hydroxide; D000818:Animals; D000881:Anthrax / Q000276:immunology / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001408:Bacillus anthracis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000096:biosynthesis; D007700:Kinetics; D009500:Neutralization Tests; D011817:Rabbits; D013171:Spores, Bacterial / Q000276:immunology; D016019:Survival Analysis; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000336:Aerosols; D022122:Anthrax Vaccines; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D000536:Aluminum Hydroxide