Decolorization of azo dyes by Rhodobacter sphaeroides.
Rhodobacter sphaeroides AS1.1737 decolorized more than 90% of several azo dyes (200 mg dyes l(-1)) in 24 h. The optimal culture conditions were: anaerobic illumination (1990 1x), peptone as carbon source, temperature 35-40 degrees C and pH 7-8. Intracellular crude enzyme from this strain had azoreductase activity, optimized temperature as 45-50 degrees C, and decolorization kinetics which were consistent with a ping-pong mechanism.
Song
Zhi-yong
ZY
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, PR China. songzhy232@yahoo.com.cn
Zhou
Ji-ti
JT
Wang
Jing
J
Yan
Bin
B
Du
Cui-hong
CH
D001391:Azo Compounds / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D003116:Color; D004396:Coloring Agents / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D009601:Nitroreductases; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*; D018508:Water Purification / Q000379:methods*
D001391:Azo Compounds; D004396:Coloring Agents; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase