title: Sequencing of Neisseria meningitidis penA gene: the key to success in defining penicillin G breakpoints.
abstract: Testing of susceptibility to penicillin G by E-test and sequencing of an internal fragment of the penA gene were done for 43 meningococcal strains. Those strains for which the MIC was >/=0.094 micro g/ml showed mosaic alleles, so 0.094 micro g/ml is suggested as the penicillin G intermediate breakpoint when E-test is used.
authors.affiliation: Reference Laboratory for Meningococci, National Center of Microbiology, Institute of Health Carlos III, Madrid, Spain.
authors.forename: Luisa
authors.initials: L
authors.lastname: Arreaza
authors.forename: Celia
authors.initials: C
authors.lastname: Salcedo
authors.forename: Belén
authors.initials: B
authors.lastname: Alcalá
authors.forename: María José
authors.initials: MJ
authors.lastname: Uría
authors.forename: Raquel
authors.initials: R
authors.lastname: Abad
authors.forename: Rocío
authors.initials: R
authors.lastname: Enríquez
authors.forename: Julio A
authors.initials: JA
authors.lastname: Vázquez
mesh_terms: D000483:Alleles; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000187:drug effects / Q000235:genetics*; D010400:Penicillin G / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D011110:Polymorphism, Genetic / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; D000667:Ampicillin; D010400:Penicillin G