Effect of petrolatum coating on the rate of occlusion of ameroid constrictors in the peritoneal cavity.
OBJECTIVE
To document rate of closure and degree of inflammation associated with petrolatum coated (PCA) and non-coated ameroid constrictors (NCA) in the peritoneal cavity.


METHODS
Experimental study.


METHODS
18 Sprague-Dawley rats.


METHODS
Thirty-six ameroid constrictors (AC; 5 mm) were digitally scanned and luminal area measured. Rats were anesthetized, and 1 PCA and 1 NCA were inserted in the peritoneal cavity by median celiotomy. Rats were euthanatized at 2 weeks (6 rats), 4 weeks (6), or 6 weeks (6) after surgery. AC were harvested, digitally scanned, and luminal area determined. Inflammation associated with the AC was subjectively graded (1-5). The effects of petrolatum coating on luminal area measurements and inflammatory score were statistically analyzed.


RESULTS
Closure of AC occurred most rapidly during the first 2 weeks, but luminal area decreased only 32% at 6 weeks after implantation. There was no significant difference in rate of closure for PCA compared to NCA at 2, 4, or 6 weeks. Inflammation scores were not significantly different between PCA and NCA.


CONCLUSIONS
Petrolatum coating did not slow the rate of closure of AC in the peritoneal cavity.


CONCLUSIONS
The lack of closure of AC supports the conclusion that vascular attenuation is not dependent on luminal constriction alone. Petrolatum coating did not slow the rate of casein expansion and is unlikely to be clinically useful.
Adin
Christopher A
CA
Department of Surgical and Radiological Sciences, Center for Laboratory Animal Services, School of Veterinary Medicine, University of California--Davis, Davis, CA, USA. adinc@mail.vetmed.ufl.edu
Gregory
Clare R
CR
Kyles
Andrew E
AE
Griffey
Stephen M
SM
Kendall
Lon
L
D000818:Animals; D001672:Biocompatible Materials; D002364:Caseins; D002371:Cat Diseases / Q000601:surgery; D002415:Cats; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000601:surgery; D004285:Dogs; D020100:Hydrogels; D006975:Hypertension, Portal / Q000601:surgery / Q000662:veterinary*; D008297:Male; D009823:Ointment Bases; D010529:Peritoneal Cavity / Q000601:surgery*; D010577:Petrolatum; D011168:Portal System / Q000002:abnormalities* / Q000601:surgery; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013537:Sutures / Q000662:veterinary*; D014945:Wound Healing
D001672:Biocompatible Materials; D002364:Caseins; D020100:Hydrogels; D009823:Ointment Bases; D010577:Petrolatum; C009719:ameroid