Serum alcohol concentrations in trauma patients determined by immunoassay versus gas chromatography.
There has been an extensive discussion in the forensic toxicology community regarding the effect of trauma on the enzymatic method for ethanol analysis. There is a paucity of information in the literature that addresses this question. This study was designed to compare the Dade Behring Dimension enzymatic method with the reliable gas chromatographic method. The blood samples collected at the same time from trauma patients were analyzed by both methods. The result of the study shows no significant quantitative difference between the enzymatic and gas liquid chromatographic (GLC) methods. Also, the enzymatic method did not show any false positive ethanol in cases where the GLC method showed a negative finding.
Winek
Charles L
CL
Pittsburgh Criminalistics Laboratory, Duquesne University, 1320 Fifth Avenue, Pittsburgh, PA 15219, USA. drwinek@aol.com
Wahba
Wagdy W
WW
Windisch
Rita M
RM
Winek
Charles L
CL
D002492:Central Nervous System Depressants / Q000097:blood*; D002849:Chromatography, Gas; D000431:Ethanol / Q000097:blood*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D014947:Wounds and Injuries / Q000097:blood*
D002492:Central Nervous System Depressants; D000431:Ethanol