title: Immunodetection of osteoadherin in murine tooth extracellular matrices.
abstract: An antiserum was generated from synthetic peptides highly conserved between different mammalian species to immunolocalise the small leucine-rich proteoglycan osteoadherin (OSAD) in murine teeth. In 19-day-old embryos of rats and mice, a positive staining was found in incisor predentin and alveolar bone surrounding developing incisors and molars. In newborns, OSAD was detected at the tip of the first molar cusp where it accumulated in predentin concomitantly with odontoblast differentiation. In 2-day-old rats and mice, in the first molar, immunostaining revealed positive predentin, enamel matrix close to the apical pole of ameloblasts and a strong signal in dentin. At this stage, OSAD was detected in predentin in the second molar. Ultrastructural immunocytochemistry showed gold particles associated with collagen fibres in predentin and in foci at the dentin mineralisation front. Gold particles were also detected near the secretory pole of ameloblasts where enamel crystallites elongate. No staining was detected in pulp tissue and dental follicle. Restriction of OSAD expression to the extracellular matrix of bone, dentin and enamel suggests a role of this proteoglycan in the organisation of mineralised tissues.
authors.affiliation: Laboratoire du Développement des Tissus Dentaires, EA 1892, IFR 62, Faculté d'Odontologie, Rue G. Paradin, 69372, Lyon cedex 08, France.
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Couble
authors.forename: Françoise
authors.initials: F
authors.lastname: Bleicher
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Farges
authors.forename: Simone
authors.initials: S
authors.lastname: Peyrol
authors.forename: Marion
authors.initials: M
authors.lastname: Lucchini
authors.forename: Henry
authors.initials: H
authors.lastname: Magloire
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Staquet
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000378:metabolism; D000918:Antibody Specificity; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000737:chemistry / Q000196:embryology / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014070:Tooth / Q000737:chemistry* / Q000196:embryology / Q000648:ultrastructure; D014083:Tooth Germ / Q000737:chemistry / Q000196:embryology / Q000648:ultrastructure
chemical_list: D000906:Antibodies; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; C113360:osteoadherin