Hypoglycemic effect of Rehmannia glutinosa oligosaccharide in hyperglycemic and alloxan-induced diabetic rats and its mechanism.
The hypoglycemic and anti-diabetic effect of Rehmannia glutinosa oligosaccharide (ROS) in glucose-induced hyperglycemic and alloxan-induced diabetic rats and its mechanism was investigated in this paper. It was found that pretreatment of ROS in normal rats with 100 mg/kg for 3 days, i.p., induced a partial prevention of hyperglycemia caused by glucose (2g/kg, i.p.), while when hyperglycemia was induced in adrenalectomized (ADX) rats, the preventive effect of ROS on hyperglycemia was lost. In alloxan-induced diabetic rats, ROS (100 mg/kg for 15 days, i.p.) showed a significant decrease in blood glucose level and hepatic glucose-6-phosphatase activity with an increase in hepatic glycogen content. Furthermore, ROS raised plasma insulin level and lowered plasma corticosterone level in alloxan-induced diabetic rats. The results indicated that oligosaccharide of Rehmannia glutinosa Libosch. exerted a significant hypoglycemic effect in normal and alloxan-induced diabetic rats. The regulatory mechanism of ROS on glucose metabolism was adrenal dependent and had a close relation with the neuroendocrine system.
Zhang
Ruxue
R
Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050, PR China. empriqqq@public.lz.gs.cn
Zhou
Jinhuang
J
Jia
Zhengping
Z
Zhang
Yongxiang
Y
Gu
Guoming
G
D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003345:Corticosterone / Q000037:antagonists & inhibitors / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005260:Female; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors; D006943:Hyperglycemia / Q000188:drug therapy*; D007328:Insulin / Q000096:biosynthesis / Q000097:blood; D008112:Liver Glycogen / Q000096:biosynthesis; D008516:Medicine, Chinese Traditional; D009844:Oligosaccharides / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D032267:Rehmannia
D001786:Blood Glucose; D007328:Insulin; D008112:Liver Glycogen; D009844:Oligosaccharides; D010936:Plant Extracts; D000496:Alloxan; D005952:Glucose-6-Phosphatase; D003345:Corticosterone