title: Real-time blood plasma polymerase chain reaction for management of disseminated adenovirus infection.
abstract: We evaluated the usefulness of quantifying blood plasma adenovirus DNA loads for the management of adenovirus infection. Quantification of adenovirus A, B, and C DNA loads was done with real-time polymerase chain reaction (PCR) assays. Blood plasma specimens obtained from 44 immunocompromised patients were screened prospectively with this method. PCR findings for 36 patients were negative, and none of the patients developed disseminated adenoviral disease. PCR findings for 8 patients were positive; all 8 had invasive adenoviral disease and were treated with cidofovir. Sequential measurements of adenovirus DNA loads were performed to monitor the effect of cidofovir therapy. Decrease in the blood plasma DNA load was significantly higher in patients with a good response to cidofovir than in patients with a poor response and was therefore correlated with survival. Detection of adenovirus DNA in blood plasma appears to be useful for identifying patients at risk for invasive disease. Moreover, quantification of adenovirus DNA loads in plasma is helpful for monitoring the efficacy of antiviral therapy.
authors.affiliation: Laboratoire de Virologie, Centre Hospitalo-Universitaire Necker-Enfants Malades, Paris, France. marianne.leruez@nck.ap-hop-paris.fr
authors.forename: Marianne
authors.initials: M
authors.lastname: Leruez-Ville
authors.forename: Véronique
authors.initials: V
authors.lastname: Minard
authors.forename: Florence
authors.initials: F
authors.lastname: Lacaille
authors.forename: Agnès
authors.initials: A
authors.lastname: Buzyn
authors.forename: Eric
authors.initials: E
authors.lastname: Abachin
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blanche
authors.forename: François
authors.initials: F
authors.lastname: Freymuth
authors.forename: Christine
authors.initials: C
authors.lastname: Rouzioux
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification* / Q000502:physiology; D000258:Adenovirus Infections, Human / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D000293:Adolescent; D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004279:DNA, Viral / Q000097:blood*; D016903:Drug Monitoring; D006801:Humans; D007223:Infant; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D003596:Cytosine; D000077404:Cidofovir