title: [Fluconazole resistance in Candida albicans assayed by PCR fingerprinting with M13 prime].
abstract: OBJECTIVE
  To understand the molecular and genetic mechanism underlying fluconazole resistance in Candida albicans by PCR fingerprinting with M13 primer.
  
  
  METHODS
  Paper disc diffusion method was employed for assay of fluconazole resistance in 41 clinical isolates of Candida albicans, followed by PCR fingerprinting with M13 primer to study the gel patterns with cluster analysis using neighbor joining (NJ) method performed with RAPD200 software.
  
  
  RESULTS
  Of the 41 clinical isolates, 11 strains (26.8%) were fluconazole-sensitive, 8 (19.5%) fluconazole-dependent and 22 (53.7%) fluconazole-resistance. Two to twelve bands could be observed among these strains, and the gel patterns revealed by cluster analysis were associated with the reactions of the strains against fluconazole and the location of infection.
  
  
  CONCLUSIONS
  There is high prevalence of fluconazole resistance in clinical Candida albicans isolates, and PCR fingerprinting with M13 primer is convenient for assay of fluconazole resistance and molecular epidemiological study of Candida albicans.
authors.affiliation: Department of Cell Biology, First Military Medical University, Guangzhou 510515, China. wangybgz@163.net
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Guo
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Shen-Qiu
authors.initials: SQ
authors.lastname: Luo
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000032:analysis; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole