Comparative study of the effects of isatin, an endogenous MAO-inhibitor, and selegiline on bradykinesia and dopamine levels in a rat model of Parkinson's disease induced by the Japanese encephalitis virus.
We previously reported that exogenously administered isatin, an endogenous monoamine oxidase (MAO) inhibitor, significantly increased acetylcholine (ACh) and dopamine (DA) levels in the rat striatum. Selegiline [(-)-deprenil] was developed as a MAO-B inhibitor more than 30 years ago and widely used in the treatment of Parkinson's disease. Effects of isatin or selegiline were investigated in Japanese encephalitis virus (JEV)-induced post-encephalitic parkinsonism rats by a pole test for detecting motor activity and by the determination of biogenic amine levels. Motor activity of JEV-induced rats receiving isatin (100 mg/kg per day for 1 week, i.p.) or selegiline (0.2 mg/kg per day for 1 week, i.p.) was significantly improved compared with that of untreated JEV-infected rats. Both isatin and selegiline prevented the decrease in striatal DA levels in JEV-rats. The increased turnover of DA (DOPAC/DA) induced by JEV was significantly inhibited by isatin, but not by selegiline. These results suggested that exogenously administered isatin and selegiline can improve JEV-induced parkinsonism by increasing DA concentrations in the striatum.
Hamaue
N
N
The Research Institute of Personalized Health Sciences, Health Sciences University of Hokkaido, 061-0293 Ishikari-Tobetsu, Japan.
Minami
M
M
Terado
M
M
Hirafuji
M
M
Endo
T
T
Machida
M
M
Hiroshige
T
T
Ogata
A
A
Tashiro
K
K
Saito
H
H
Parvez
S H
SH
D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D004664:Encephalitis Virus, Japanese; D018476:Hypokinesia / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D007510:Isatin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D010300:Parkinson Disease / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D051381:Rats; D012642:Selegiline / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use
D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D007510:Isatin; D004298:Dopamine