title: [Microbiological and molecular genetic characterization of Mycobacterium tuberculosis strains isolated from patients with multiorgan and generalized tuberculosis].
abstract: The paper presents the microbiological and molecular genetic characteristics of Mycobacterium tuberculosis (MBT) isolated from multiple lesion foci in 26 patients with multiorgan and generalized tuberculosis. Cultures of MBT of the family Beijing were isolated from the pathological specimens taken from 17 (65.4%) patients; those with individual genotypes were in 9 (34.6%) patients. The study indicated that cultured MBT from different types of pathological material from 25 of the 26 patients had identical spoligotypes irrespective of their clinical diagnosis. At the same time minor profile IS6110 changes might occur in the Mycobacteria of a patient during persistence. It may be concluded that multiple foci of tuberculous affliction in the patients are of unique origin and have the mechanism of development, associated with endogenous reactivation of infection.
authors.forename: T F
authors.initials: TF
authors.lastname: Otten
authors.forename: O V
authors.initials: OV
authors.lastname: Narvskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Oleĭnik
authors.forename: I V
authors.initials: IV
authors.lastname: Mokrousov
authors.forename: E V
authors.initials: EV
authors.lastname: Limeshchenko
authors.forename: A A
authors.initials: AA
authors.lastname: Viazovaia
authors.forename: B I
authors.initials: BI
authors.lastname: Vishnevskiĭ
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D013166:Spondylitis / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology*; D014388:Tuberculosis, Lymph Node / Q000382:microbiology; D014394:Tuberculosis, Osteoarticular / Q000382:microbiology; D014399:Tuberculosis, Spinal / Q000382:microbiology; D014401:Tuberculosis, Urogenital / Q000382:microbiology; D014556:Urine / Q000382:microbiology