title: No evidence for linkage and/or association of human alpha2-HS glycoprotein gene with bone mineral density variation in Chinese nuclear families.
abstract: Osteoporosis is an important health problem in the world. Alpha2-HS glycoprotein (AHSG) is involved in bone formation and metabolism and has been considered as an important candidate gene for osteoporosis. In this study, we simultaneously tested linkage and/or association of the AHSG gene with the variation of bone mineral density (BMD), an important risk factor for osteoporosis. A sample of 1,260 subjects from 401 Chinese nuclear families (including both parents and their daughters) were studied. The daughters' ages ranged from 20 to 45 years. All the subjects were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) at polymorphic Sac I site inside the exon 7 of the AHSG gene. This polymorphism involves a nucleotide substitution of C to G at the middle nucleotide of the codon at amino acid position 238, resulting in the replacement of threonine (ACC) with serine (AGC). BMD was measured at the lumbar spine and hip region by dual-energy X-ray absorptiometry (DXA). Using the QTDT (quantitative trait transmission disequilibrium test), we found no significant results for association or linkage between the AHSG gene and BMD variation at the spine or hip. Our data provided no evidence to support the AHSG gene as a quantitative trait locus (QTL) for the BMD variation in a Chinese population.
authors.affiliation: Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR China.
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Liu
authors.forename: D K
authors.initials: DK
authors.lastname: Jiang
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.forename: M X
authors.initials: MX
authors.lastname: Li
authors.forename: Y J
authors.initials: YJ
authors.lastname: Qin
authors.forename: W X
authors.initials: WX
authors.lastname: Jian
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: H W
authors.initials: HW
authors.lastname: Deng
mesh_terms: D015502:Absorptiometry, Photon; D000328:Adult; D044466:Asian People / Q000235:genetics*; D001798:Blood Proteins / Q000235:genetics*; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005260:Female; D008040:Genetic Linkage; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009679:Nuclear Family; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein; D004247:DNA