title: The content of heat shock protein 47 (HSP47), a collagen-specific stress protein, changes with gravitational conditions in skeletal muscle.
abstract: It is well known that unloading of skeletal muscle with spaceflight or tail suspension leads rat soleus muscle atrophy. Previously, we reported that one of small heat shock protein (sHSP), alpha B-crystallin shows an early dramatic decrease in atrophied rat soleus muscle (Atomi et al, 1991). In this report, we focused to study the gravitational responses of another HSP, which may be reactive to the gravity. HSP47, a collagen-specific stress protein, has been postulated to be a collagen-specific molecular chaperone localized in the ER (Nagata et al, 1992). Western blot analysis revealed that HSP47 in slow skeletal muscle decreases at 5 days after tail suspension (TS) and increased at 5 days recovery after 10 days of TS as compared with the control level. Hypothetically, HSP47 in slow soleus muscle increases at 5 days after hypergravity (HG) induced by the centrifugation. The content of HSP47 in soleus muscle was strongly affected by gravity conditions.
authors.affiliation: Department of Life Sciences, The Graduate School of Arts & Sciences, The University of Tokyo.
authors.forename: Asami
authors.initials: A
authors.lastname: Oguro
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakurai
authors.forename: Mayumi
authors.initials: M
authors.lastname: Otawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Okuno
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Atomi
mesh_terms: D000818:Animals; D050958:HSP47 Heat-Shock Proteins / Q000378:metabolism*; D019417:Hindlimb Suspension; D018471:Hypergravity; D008297:Male; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation
chemical_list: D050958:HSP47 Heat-Shock Proteins