title: Soy protein diet ameliorates renal nitrotyrosine formation and chronic nephropathy induced by puromycin aminonucleoside.
abstract: It has been shown that reactive oxygen species are involved in chronic puromycin aminonucleoside (PAN) induced nephrotic syndrome (NS) and that a 20% soy protein diet reduces renal damage in this experimental model. The purpose of the present work was to investigate if a 20% soy protein diet is able to modulate kidney nitrotyrosine formation and the activity of renal antioxidant enzymes (catalase, glutathione peroxidase, Cu,Zn- or Mn-superoxide dismutase) which could explain, at least in part, the protective effect of the soy protein diet in rats with chronic NS induced by PAN. Four groups of rats were studied: (1) Control rats fed 20% casein diet, (2) Nephrotic rats fed 20% casein diet, (3) Control rats fed 20% soy protein diet, and (4) Nephrotic rats fed 20% soy protein diet. Chronic NS was induced by repeated injections of PAN and rats were sacrificed at week nine. The soy protein diet ameliorated proteinuria, hypercholesterolemia, and the increase in serum creatinine and blood urea nitrogen observed in nephrotic rats fed 20% casein diet. Kidney nitrotyrosine formation increased in nephrotic rats fed 20% casein diet and this increase was ameliorated in nephrotic rats fed 20% soy protein diet. However, the soy protein diet was unable to modulate the antioxidant enzymes activities in control and nephrotic rats fed 20% soy protein diet. Food intake was similar in the two diet groups. The protective effect of a 20% soy protein diet on renal damage in chronic nephropathy induced by PAN was associated with the amelioration in the renal nitrotyrosine formation but not with the modulation of antioxidant enzymes.
authors.affiliation: Department of Biology, Faculty of Chemistry, Universidad Nacional Autónoma de México (UNAM), 04510, D.F., México, Mexico
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverrí
authors.forename: Diana
authors.initials: D
authors.lastname: Barrera
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: Omar N
authors.initials: ON
authors.lastname: Medina-Campos
authors.forename: Cristino
authors.initials: C
authors.lastname: Cruz
authors.forename: Fernanda
authors.initials: F
authors.lastname: Murguía
authors.forename: César
authors.initials: C
authors.lastname: Juárez-Nicolás
authors.forename: Ricardo
authors.initials: R
authors.lastname: Correa-Rotter
authors.forename: Nimbe
authors.initials: N
authors.lastname: Torres
authors.forename: Armando R
authors.initials: AR
authors.lastname: Tovar
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D004032:Diet; D004435:Eating / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D007677:Kidney Function Tests; D008055:Lipids / Q000097:blood; D008297:Male; D011507:Proteinuria / Q000139:chemically induced / Q000378:metabolism; D011692:Puromycin Aminonucleoside / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D030262:Soybean Proteins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: D000963:Antimetabolites; D000975:Antioxidants; D008055:Lipids; D030262:Soybean Proteins; C002744:3-nitrotyrosine; D014443:Tyrosine; D011692:Puromycin Aminonucleoside; D002784:Cholesterol; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase