Feasibility of using low-volume tissue samples for gene expression profiling of advanced non-small cell lung cancers.
OBJECTIVE
The majority of patients with non-small cell lung cancer (NSCLC) present at an advanced clinical stage, when surgery is not a recommended therapeutic option. In such cases, tissues for molecular research are usually limited to the low-volume samples obtained at the time of diagnosis, usually via fine-needle aspiration (FNA). We tested the feasibility of performing gene expression profiling of advanced NSCLCs using amplified RNA from lung FNAs.


RESULTS
A total of 46 FNAs was tested, of which 18 yielded RNA of sufficient quality for microarray analysis. Expression profiles of these 18 samples were compared with profiles of 17 pairs of tumor and normal lung tissues that had been surgically obtained. Using a variety of unsupervised and supervised analytical approaches, we found that the FNA profiles were highly distinct from the normal samples and similar to the tumor profiles.


CONCLUSIONS
We conclude that when RNA amplification is successful, gene expression profiles from NSCLC FNAs can determine malignancy and suggest that with additional refinement and standardization of sample collection and RNA amplification protocols, it will be possible to conduct additional and more detailed molecular analysis of advanced NSCLC using lung FNAs.
Lim
Elaine H
EH
Department of Hematology-Oncology, National University Hospital, Singapore. mdclime@nus.edu.sg
Aggarwal
Amit
A
Agasthian
Thirugananam
T
Wong
Poo-Sing
PS
Tan
Christie
C
Sim
Eugene
E
Tan
Lenny
L
Goh
Poh-Sun
PS
Wang
Shih-Chang
SC
Khoo
Kay-Leong
KL
Mukherjee
Amartya
A
Khoo
See-Meng
SM
Chua
Gerald
G
Nilsson
Barbro
B
Lee
Kang-Hoe
KH
Tan
Patrick
P
D001707:Biopsy, Needle; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012313:RNA