title: Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells.
abstract: Age-related decrease in bone formation is well described. However, the cellular causes are not known. Thus, we have established cultures of bone marrow stromal cells (MSC) from young (aged 18-29 years, n = 6) and old (aged 68-81 years, n = 5) donors. MSC were serially passaged until reaching maximal life span. Cell growth, markers of cellular senescence, and osteogenic and adipogenic potential were determined in early-passage and late-passage cells established from young and old donors. MSC from old donors exhibited a decreased maximal life span compared with cells from young donors (24 +/- 11 population doublings [PD] vs 41 +/- 10 PD, P < 0.05) and mean PD rate was lower in old donor cells (0.05 +/- 0.02 PD/day) compared with young donor cells (0.09 +/- 0.02 PD/day) (P < 0.05). No differences were detected in number of senescence-associated beta-galactosidase positive (SA beta-gal+) cells and mean telomere length in early-passage cells obtained from young and old donors. However, MSC from old donors exhibited accelerated senescence evidenced by increased number of SA beta-gal+ cells per PD as compared with young (4% per PD vs 0.4% per PD, respectively). MSC from young and old donors were able to form similar amounts of mineralized matrix in vitro and of normal lamellar bone in vivo. In adipogenic medium similar numbers of adipocytes formed in cultures of young and old donors. In conclusion, aging is associated with decreased proliferative capacity of osteoprogenitor cells, suggesting that decreased osteoblastic cell number, and not function, leads to age-related decrease in bone formation.
authors.affiliation: University Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Karin
authors.initials: K
authors.lastname: Stenderup
authors.forename: Jeannette
authors.initials: J
authors.lastname: Justesen
authors.forename: Christian
authors.initials: C
authors.lastname: Clausen
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D048429:Cell Size; D017690:Cell Transplantation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D005260:Female; D006801:Humans; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D040521:Subcutaneous Tissue / Q000033:anatomy & histology; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D015415:Biomarkers; D019098:Telomerase; D001616:beta-Galactosidase