title: Characterization in maize of ZmTIP2-3, a root-specific tonoplast intrinsic protein exhibiting aquaporin activity.
abstract: The characterization of the tonoplast intrinsic protein ZmTIP2-3 cDNA isolated from maize roots is reported. ZmTIP2-3 belongs to the TIP2 group according to the present nomenclature. The aquaporin function of ZmTIP2-3 protein was demonstrated using expression in X. laevis oocytes. Northern blot analyses revealed that ZmTIP2-3 was specifically expressed in roots. Salt and water stresses induced the accumulation of ZmTIP2-3 transcripts. By contrast, no effect of ABA was observed. An oscillation of ZmTIP2-3 transcript amount during the day-night cycle was observed with some typical features of genes regulated by a circadian mechanism.
authors.affiliation: Institut de Biotechnologie des Plantes, Bât. 630, CNRS/UMR 8618, Université Paris-Sud, F-91405 Orsay Cedex, France.
authors.forename: Félicie
authors.initials: F
authors.lastname: Lopez
authors.forename: Agnès
authors.initials: A
authors.lastname: Bousser
authors.forename: Igor
authors.initials: I
authors.lastname: Sissoëff
authors.forename: Jackson
authors.initials: J
authors.lastname: Hoarau
authors.forename: Aline
authors.initials: A
authors.lastname: Mahé
mesh_terms: D020346:Aquaporins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
chemical_list: D020346:Aquaporins; D017931:DNA Primers; D010940:Plant Proteins; D018749:RNA, Plant; D012337:RNA, Ribosomal, 18S