title: Transcriptional and translational regulation of zebrafish connexin 55.5 (zf.Cx.55.5) and connexin 52.6 (zf.Cx52.6).
abstract: Zebrafish connexin 55.5 (zf.Cx55.5) and connexin 52.6 (zf.Cx52.6) show highly restricted expression patterns in the nervous system. Both connexins are confined to subsets of neurons in the fish retina. In order to get initial answers to the questions of pattern definition in neuronal subsets, we elucidated molecular mechanisms responsible for their expression. Different upstream DNA fragments were subcloned into a pGL3-basic vector and transiently transfected in HeLa and N2A cells. Luciferase activity showed the presence of two putative promoter elements in zfCx55.5 and a promoter element in zfCx52.6 that showed different promoter activities in HeLa and N2A cells. Moreover, fusion constructs of zfCx55.5 with EGFP revealed the presence of a new isoform with an additional short exon I.
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Germany.
authors.forename: Mahbob-Ul
authors.initials: MU
authors.lastname: Hussain
authors.forename: Marian
authors.initials: M
authors.lastname: Kremer
authors.forename: Georg
authors.initials: G
authors.lastname: Zoidl
authors.forename: Rolf
authors.initials: R
authors.lastname: Dermietzel
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics
chemical_list: D017630:Connexins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins