title: Interaction assay of oligosaccharide with lectins using glycosylasparagine.
abstract: Glycosyl amino acids having natural glycan were useful for the interaction assay of oligosaccharides. A glycochip containing the whole structure of an oligosaccharide was easily prepared by the immobilization of the glycosyl amino acid. Furthermore, fluorescence probes were introduced into the glycosyl amino acid while maintaining the whole structure of oligosaccharide. By using these labeled oligosaccharides, fluorescence polarization (FP) and fluorescence correlation spectroscopy (FCS) analyzed the carbohydrate-lectin interaction in a solution assay system.
authors.affiliation: The Noguchi Institute, 1-8-1, Kaga, Itabashi-ku, 173-0003, Tokyo, Japan. mmizuno@noguchi.or.jp
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mizuno
authors.forename: Midori
authors.initials: M
authors.lastname: Noguchi
authors.forename: Mie
authors.initials: M
authors.lastname: Imai
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Motoyoshi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Inazu
mesh_terms: D000818:Animals; D001216:Asparagine / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005260:Female; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology
chemical_list: D037102:Lectins; D009844:Oligosaccharides; D001216:Asparagine