[Free radicals in mercury-resistant bacteria indicate a novel metabolic pathway].
A mercury resistant-soil bacterium P.10.15, identified as a close relative of Pseudomonas veronii, was shown to accumulate a specific compound in the stationary phase of growth. This compound is converted to a long-lived free radical under oxidizing conditions, as registered by its EPR signal at room temperature. The compound was purified by ion-exchange and gel-filtration chromatography and identified by mass spectroscopy, 2D NMR, and EPR as a trisaccharide beta-D-GlcpNOH,CH3-(1-->6)-alpha-D-Glcp-(1-->1)-alpha-D-Glcp, or, in other words, as 6-O-(2-deoxy-2-[N-methyl]hydroxylamino-beta-D- glucopyranosyl)-alpha-alpha-trehalose, previously discovered in Micrococcus luteus (lysodeikticus) and named lysodektose. The compound is suggested to be a novel intermediate of a previously unknown basic metabolic pathway of trehalose transformation in bacteria, a potential target for antibacterial drug development.
Ostrovskiĭ
D N
DN
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia. ost@inbi.ras.ru
Demina
G R
GR
Biniukov
V I
VI
Shashkov
A S
AS
Schloter
M
M
D002850:Chromatography, Gel; D024881:Drug Resistance, Bacterial; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism; D013058:Mass Spectrometry; D008628:Mercury / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000187:drug effects* / Q000378:metabolism*; D012988:Soil Microbiology; D014199:Trehalose / Q000378:metabolism; D014312:Trisaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D005609:Free Radicals; D014312:Trisaccharides; C063139:lysodektose; D014199:Trehalose; D008628:Mercury