title: Notes on theory and experimental conditions behind two-photon excitation microscopy.
abstract: This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.
authors.affiliation: Department of Physics, University of Genoa, 16146 Genoa, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Esposito
authors.forename: Federico
authors.initials: F
authors.lastname: Federici
authors.forename: Cesare
authors.initials: C
authors.lastname: Usai
authors.forename: Fabio
authors.initials: F
authors.lastname: Cannone
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Chirico
authors.forename: Maddalena
authors.initials: M
authors.lastname: Collini
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro
mesh_terms: D004247:DNA / Q000648:ultrastructure*; D049673:History, 20th Century; D018613:Microscopy, Confocal / Q000266:history / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000266:history / Q000379:methods*; D008962:Models, Theoretical; D017785:Photons; D012680:Sensitivity and Specificity; D014584:User-Computer Interface
chemical_list: D004247:DNA