[Structural and functional state of microsomal membranes in the rat brain cortex during long-term ethanol consumption].
The range of the Na+, K(+)-ATPase functional stability in microsomal fraction of rat brain cortex under long-term chronic ethanol (15%, v/v) consumption was ascertained. The enzyme activity decreased only after 15 months of alcoholisation on the background of the stable structural membrane characteristics (on the basis of the intrinsic and 1-anilinonaphthalene-8-sulfonate fluorescence parameters) and SH-content in postmitochondrial supernatant. The cellular homeostatic mechanisms under ethanol effect are discussed.
Mishchuk
D O
DO
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Kaplia
A A
AA
D000284:Administration, Oral; D000437:Alcoholism / Q000201:enzymology / Q000503:physiopathology*; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology*; D002540:Cerebral Cortex / Q000201:enzymology / Q000502:physiology*; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage; D005260:Female; D008861:Microsomes / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D000817:Anilino Naphthalenesulfonates; D000431:Ethanol; C027132:1-anilino-8-naphthalenesulfonate; D000254:Sodium-Potassium-Exchanging ATPase