title: Effect of gravity stress on fidelity of DNA double-strand break repair.
abstract: DNA double strand break (DSB) causes many cytotoxic effects such as cellular lethality, somatic mutation, and carcinogenesis. Fidelity of DSB repair is a important factor that determines the quality of genomic stability. It is known that the most of DSBs are properly repaired on the earth, however, little is known whether those are rejoined at the same fidelity even under the space environment. One of the DSB repair pathway, homologous recombination (HR), allows the cells to repair their DSBs with error free. Therefore, the efficiency of HR is a good index to assess the fidelity of DSB repair. In order to clarify the effect of gravity stress on HR pathway, we established a cell line that can detect a site-specific DNA repair via HR. The cells carrying a reporter construct for HR were incubated under hypergravity condition after induction of site specific DSB. Our preliminary results suggest that the gravity stress may affect the HR efficiency.
authors.affiliation: Dept. Environmental Sciences, Faculty of Science, Ibaraki Univ.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tauchi
authors.forename: Eigo
authors.initials: E
authors.lastname: Matsumoto
authors.forename: Kenta
authors.initials: K
authors.lastname: Iijima
authors.forename: Daisuke
authors.initials: D
authors.lastname: Mochizuki
authors.forename: Kenshi
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ichimasa
mesh_terms: D018797:Cell Cycle Proteins; D002460:Cell Line; D002470:Cell Survival; D004249:DNA Damage; D004260:DNA Repair; D006112:Gravitation; D006801:Humans; D018471:Hypergravity; D009687:Nuclear Proteins; D011995:Recombination, Genetic
chemical_list: D018797:Cell Cycle Proteins; C112156:NBN protein, human; D009687:Nuclear Proteins