Transcript abundance of rml1, encoding a putative GT1-like factor in rice, is up-regulated by Magnaporthe grisea and down-regulated by light.
We isolated and sequenced both genomic DNA and cDNA clones, which encoded a putative GT1-like protein with 385 amino acids, from cultivated rice (Oryza sativa ssp. indica). This protein shows significant amino acid sequence similarities with trihelix DNA-binding GT-1a/B2F and GT-1 factors that were identified in dicot plants. Northern blotting analysis indicated that the transcript of the rice GT-1 factor in seedling was up-regulated by the rice blast fungus Magnaporthe grisea, down-regulated by various continuous light conditions and expressed rhythmically in light/dark cycles. This GT1-like factor gene was therefore designated as rml1 (rice gene regulated by M. grisea and light). The putative RML1 protein, encoded by this single copy gene, is thus identified as a new member of the plant-specific GT family of transcription factors in rice.
Wang
Rong
R
National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
Hong
Guofan
G
Han
Bin
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D020082:Magnaporthe / Q000254:growth & development*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000382:microbiology / Q000528:radiation effects; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000528:radiation effects; D015854:Up-Regulation
D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D018749:RNA, Plant