Hydrodynamic properties of oxidized extracellular polysaccharides from Erwinia chrysanthemi spp.
The molecular weights of the native polysaccharides of Erwinia chrysanthemi strains range from 1.8 to 7.1 x 10(6) and their hydrodynamic properties are those of polydisperse, polyanionic biopolymers with pseudoplastic, non-thixotropic flow characteristics in aqueous solutions. The effect on the hydrodynamic properties of the polysaccharides by adding carboxyl groups to increase the charge density is studied, with particular reference to their molecular weight (MW), viscosity and conformation. In general, it is found that periodate oxidation of the extracellular polysaccharides of E. chrysanthemi strains, Ech9Sm6 and Ech6S+, introduces little change in the hydrodynamic properties of the resulting polyaldehydes. However, bromine oxidation at neutral pH of the polyaldehydes results in polycarboxylate biopolymers that show significant reduction in MW and viscosity, but they are still characteristic polyanions.
Yang
Byung Yun
BY
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Ding
Qiong
Q
Montgomery
Rex
R
D000447:Aldehydes / Q000737:chemistry; D001704:Biopolymers; D001966:Bromine / Q000737:chemistry; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D016974:Dickeya chrysanthemi / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007477:Ions; D008027:Light; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism*; D010504:Periodic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012542:Scattering, Radiation; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000447:Aldehydes; D001704:Biopolymers; D007477:Ions; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D014867:Water; D010504:Periodic Acid; C009288:metaperiodate; D010100:Oxygen; D001966:Bromine