Partial loss-of-function alleles reveal a role for GNOM in auxin transport-related, post-embryonic development of Arabidopsis.
The Arabidopsis GNOM gene encodes an ARF GDP/GTP exchange factor involved in embryonic axis formation and polar localisation of the auxin efflux regulator PIN1. To examine whether GNOM also plays a role in post-embryonic development and to clarify its involvement in auxin transport, we have characterised newly isolated weak gnom alleles as well as trans-heterozygotes of complementing strong alleles. These genotypes form a phenotypic series of GNOM activity in post-embryonic development, with auxin-related defects, especially in the maintenance of primary root meristem activity and in the initiation and organisation of lateral root primordia. Our results suggest a model for GNOM action mediating auxin transport in both embryogenesis and post-embryonic organ development.
Geldner
Niko
N
ZMBP, Entwicklungsgenetik, Universität Tübingen, Auf der Morgenstelle 3, D-72076 Tuebingen, Germany.
Richter
Sandra
S
Vieten
Anne
A
Marquardt
Sebastian
S
Torres-Ruiz
Ramon A
RA
Mayer
Ulrike
U
Jürgens
Gerd
G
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001693:Biological Transport, Active; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018522:Gravitropism / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D007210:Indoleacetic Acids / Q000378:metabolism; D018519:Meristem / Q000254:growth & development; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development
D029681:Arabidopsis Proteins; C088087:GNOM protein, Arabidopsis; D020662:Guanine Nucleotide Exchange Factors; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators