title: Identification of a sex pheromone component of Pseudococcus cryptus.
abstract: A sex pheromone component of Pseudococcus cryptus has been isolated and identified. The crude pheromone extract obtained by airborne collection was fractionated by liquid chromatography (LC) on Florisil, and further purified by high performance liquid chromatography and preparative Gas Chromatography (GC). The pheromone component was shown to be an ester, the alcohol part of which was identical to the known alcohol moiety of the pheromone of Planococcus citri. The chemical structure was determined to be 3-isopropenyl-2,2-dimethylcyclobutylmethyl 3-methyl-3-butenoate by MS and 1H NMR analyses. The absolute configuration of the pheromone was assigned as (1R,3R) by comparison of the retention time of the alcohol derived from the P. cryptus pheromone with those of the alcohol derived from P. citri pheromone, and a synthetic sample of alcohol enriched in the (1R,3R)-enantiomer, using a chiral GC stationary phase. The structure of the pheromone was confirmed by synthesis, and by bioassays in a glasshouse.
authors.affiliation: Department of Citrus Research, Kuchinotsu, National Institute of Fruit Tree Science, Kuchinotsu, Nagasaki 859-2501, Japan. gaityuu@affrc.go.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Arai
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugie
authors.forename: Syuntaro
authors.initials: S
authors.lastname: Hiradate
authors.forename: Shigefumi
authors.initials: S
authors.lastname: Kuwahara
authors.forename: Noriaki
authors.initials: N
authors.lastname: Itagaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakahata
mesh_terms: D000818:Animals; D001681:Biological Assay; D002087:Butyrates / Q000737:chemistry* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003503:Cyclobutanes / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007313:Insecta / Q000737:chemistry* / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D012724:Sex Attractants / Q000737:chemistry* / Q000494:pharmacology
chemical_list: C482762:3-isopropenyl-2,2-dimethylcyclobutylmethyl 3-methyl-3-butenoate; D002087:Butyrates; D003503:Cyclobutanes; D012724:Sex Attractants