Human aurora-B binds to a proteasome alpha-subunit HC8 and undergoes degradation in a proteasome-dependent manner.
Human Aurora/Ipl1-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome alpha-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.
Shu
Fengjue
F
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
Guo
Shuguang
S
Dang
Yongjun
Y
Qi
Meiyan
M
Zhou
Guangjin
G
Guo
Zekun
Z
Zhang
Ying
Y
Wu
Chaoqun
C
Zhao
Shouyuan
S
Yu
Long
L
D064107:Aurora Kinase B; D064127:Aurora Kinases; D002453:Cell Cycle; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008938:Mitosis; D009097:Multienzyme Complexes / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D009097:Multienzyme Complexes; D005982:Glutathione Transferase; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D003546:Cysteine Endopeptidases; C488165:PSMA3 protein, human; D046988:Proteasome Endopeptidase Complex