The use of hydrolysis and hairpin probes in real-time PCR.
In this review we discuss the limitations of traditional PCR methods and introduce real-time PCR, a fluorescence-based detection system, as a method that overcomes these limitations. We describe the technologies involved in real-time PCR including the design of hydrolysis and hairpin probes and the practical applications of such systems.
McChlery
Susan M
SM
North Glasgow University Hospital NHS Trust, Department of Microbiology, House on the Hill, Stobhill Hospital, Balornock Road, Glasgow, G21 3UW.
Clarke
Stuart C
SC
D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012313:RNA