Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer.
The optimal chromatographic conditions for the separation of the syn- and anti-geometric isomers of cefuroxime axetil applying RP-HPLC and micellar liquid chromatography (MLC) methods were investigated. The possibility to separate diastereoisomers of syn- and anti-cefuroxime axetil was observed. Investigations were performed using three columns, two classical silicas and one with hybrid particle technology. Three aqueous-organic and one micellar mobile phases were used. The best results were achieved using micellar mobile phase. Optimization study was performed using different micellar mobile phases. MLC method is sensitive and applicable in purity and stability testing.
Zivanovic
Ljiljana
L
Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia, Yugoslavia.
Ivanovic
Ivana
I
Vladimirov
Sote
S
Zecevic
Mira
M
D019840:2-Propanol / Q000737:chemistry; D002444:Cefuroxime / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary; D007202:Indicators and Reagents; D007536:Isomerism; D012965:Sodium Chloride / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents
D007202:Indicators and Reagents; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D019840:2-Propanol; D002444:Cefuroxime; C040738:cefuroxime axetil