Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes.
The effect of 30.16 GHz millimeter wave (MMW) exposure at 1.0 and 3.5 mW/cm2 on gap junction intercellular communication (GJIC) was studied in cultured HaCaT keratinocytes, using the fluorescence recovery after photobleaching (FRAP) technique and laser confocal scanning microscopy to follow the intracellular movement of 5,6-carboxyfluorescein diacetate dye. While MMW exposure alone for 1 h at either 1.0 or 3.5 mW/cm2 did not affect GJIC, MMW exposure in combination with 5 ng/ml TPA treatment reversed TPA induced suppression of GJIC. Exposure at 1.0 mW/cm2 resulted in a partial reversal, and exposure at 3.5 mW/cm2 resulted in essentially full reversal of the TPA suppression.
Chen
Q
Q
Bioelectromagnetics Laboratory, Institute of Industrial and Environmental Hygiene, Zhejiang University School of Medicine, Hangzhou, China. cq203@zjuem.zju.edu.cn
Zeng
Q L
QL
Lu
D Q
DQ
Chiang
H
H
D002450:Cell Communication / Q000187:drug effects* / Q000528:radiation effects*; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance / Q000528:radiation effects; D036681:Fluorescence Recovery After Photobleaching; D017629:Gap Junctions / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D018613:Microscopy, Confocal; D008872:Microwaves; D010703:Phorbol Esters / Q000494:pharmacology*; D011829:Radiation Dosage
D010703:Phorbol Esters