title: Monoclonal antibodies to the recombinant protein TmpA of the Treponema pallidum.
abstract: Spleen cells from BALB/c mice immunized with recombinant TmpA were fused with mouse myeloma cells (P3/X63-Ag8), and five hybridomas secreting monoclonal antibodies were obtained. These hybridomas specifically recognize TmpA and do not cross-react with other molecules such as recombinant HBsAg of HBV and synthetic HCV core peptides. The monoclonal antibodies were IgG1 subclass and ascitic fluid from these hybridomas was purified by affinity chromatography on Protein A-Sepharose CL-4B column to isolate the IgG1 active fraction. The affinity constant of these monoclonal antibodies ranged from 6.4 x 10(8) and 1.73 x 10(10) M(-1).
authors.affiliation: Laboratorio de Anticuerpos Monoclonales, Centro de Inmunoensayo, Calle 134 y ave. 25, Apartado 6653, Cubanacán, Playa, Havana City, Cuba.
authors.forename: Adys I
authors.initials: AI
authors.lastname: Brito Moreno
authors.forename: Carmen
authors.initials: C
authors.lastname: Acosta Bas
authors.forename: Maya
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Ileana B
authors.initials: IB
authors.lastname: Baluja Conde
authors.forename: Sadys
authors.initials: S
authors.lastname: Feal Carballo
authors.forename: Luisa
authors.initials: L
authors.lastname: Martínez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology*; D002846:Chromatography, Affinity; D000939:Epitopes; D006825:Hybridomas / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013587:Syphilis / Q000175:diagnosis; D014210:Treponema pallidum / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D007074:Immunoglobulin G; D008565:Membrane Proteins; D010455:Peptides; D011994:Recombinant Proteins; C098083:TmpA protein, Treponema pallidum