Potential of laser immunoassay for detection of HIV in human blood serum and urine.
The potential of Light Scattering Immunoassay (LIA) for detection of HIV in human blood serum has been explored by monitoring the agglutination of antigen coated polystyrene particles by dynamic light scattering. ELISA tested human sera having HIV, TB, Filaria along with normal sera have been analyzed using two specific synthetic peptide antigen (SP1, SP2) and one nonspecific peptide antigen (NSP). Few paired human sera and urine samples and nonspecific (of nonHIV diseases) urine samples have also been tested using the same antigens to check the possibility of replacement of sera by urine.
Saxena
Anita
A
School of Physical Sciences, Jawaharlal Nehru University, New Delhi, India.
Roy
K B
KB
Bohidar
H B
HB
D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000097:blood / Q000652:urine; D005368:Filariasis / Q000175:diagnosis; D015488:HIV Antigens / Q000097:blood* / Q000652:urine*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000652:urine; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis
D000936:Antigen-Antibody Complex; D015488:HIV Antigens