Identification of residues of the kid toxin involved in autoregulation of the parD system.
The toxin-antitoxin system parD (kis kid) of plasmid R1 is coregulated by the coordinated action of its two gene products. Here we describe the isolation and the in vivo characterization of three single-amino-acid changes in the Kid toxin, G4E, C74Y, and E91K, that affect the coregulatory activity but preserve the toxicity of the protein.
Lemonnier
Marc
M
Centro de Investigaciones Biológicas (CSIC), 28040 Madrid, Spain.
Santos-Sierra
Sandra
S
Pardo-Abarrio
Consolación
C
Díaz-Orejas
Ramón
R
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006706:Homeostasis; D009154:Mutation; D010957:Plasmids
D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C489746:ParD protein, E coli; C489749:ParD protein, Plasmid R1; C473072:kid toxin protein, E coli plasmid R1; C078613:parD protein, Bacteria