[Establishment of protein spectrum of nasopharyngeal carcinoma cell line HNE-1 by two-dimensional polyacrylamide gel electrophoresis].
OBJECTIVE
To establish optimized two-dimensional (2-D) polyacrylamide gel electrophoresis (PAGE) for displaying the full-scale protein expression spectrum of nasopharyngeal carcinoma (NPC) cell line HNE-1, which can be instrumental for proteomic study of NPC.


METHODS
Large-scale HNE-1 cell culture was carried out from which the total protein was extracted with optimized method and separated by isoelectric focusing with linear and non-linear immobilized pH gradient (IPG) strips between different pH ranges followed by vertical SDS-PAGE. The 2-D maps of the proteins of HNE-1 cell line were then established.


RESULTS
2-D maps of the total protein of HNE-1 cell line were effectively displayed on IPG strips of different pH ranges and with optimized 2-D electrophoresis.


CONCLUSIONS
With IPG strips with overlapping pH ranges, improvement of the protein separation can be achieved in 2-D electrophoresis.
Xu
Shou-jun
SJ
Department of Pathology, First Military Medical University, Guangzhou 510515, China. ygzhi@fimmu.com
Li
Xin
X
Lü
Li-chun
LC
Li
Xue-hua
XH
D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*
D009363:Neoplasm Proteins