title: Exercise-induced protein kinase C isoform-specific activation in human skeletal muscle.
abstract: We determined whether protein kinase C (PKC) isoforms are redistributed and phosphorylated in response to acute exercise in skeletal muscle. Muscle biopsies were obtained from six healthy subjects (four women, two men; age 25 +/- 1 years) before, during, and after 60 min of one-leg cycle ergometry at approximately 70% VO(2peak). Exercise for 30 and 60 min was associated with a three- and fourfold increase in PKC-zeta/lambda abundance and a four- and threefold increase in phosphorylation, respectively, in total membranes (P < 0.05) and a decrease in PKC-zeta/lambda phosphorylation in cytosolic fractions. During exercise recovery, PKC-zeta/lambda abundance and phosphorylation remained elevated. PKC-zeta/lambda abundance and phosphorylation were increased in nonexercised muscle upon cessation of exercise, indicating a systemic response may contribute to changes in PKC abundance and phosphorylation. Exercise did not change PKC-delta or -epsilon abundance or phosphorylation in either the cytosolic or total membrane fraction. In conclusion, exercise is associated with an isoform-specific effect on PKC. PKC-zeta/lambda are candidate PKC isoforms that may play a role in the regulation of exercise-related changes in metabolic and gene-regulatory responses.
authors.affiliation: Department of Surgical Sciences, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Sebastio
authors.initials: S
authors.lastname: Perrini
authors.forename: Jan
authors.initials: J
authors.lastname: Henriksson
authors.forename: Juleen R
authors.initials: JR
authors.lastname: Zierath
authors.forename: Ulrika
authors.initials: U
authors.lastname: Widegren
mesh_terms: D000328:Adult; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta; C497258:PRKCD protein, human; C497255:PRKCE protein, human