title: Cellular polyamines of the acidophilic, thermophilic and thermoacidophilic archaebacteria, Acidilobus, Ferroplasma, Pyrobaculum, Pyrococcus, Staphylothermus, Thermococcus, Thermodiscus and Vulcanisaeta.
abstract: Cellular polyamines of newly isolated acidophilic, thermophilic and thermoacidophilic archaebacteria were investigated for the chemotaxonomic significance of polyamine distribution profiles. In addition to spermidine, spermine and agmatine, a quaternary branched penta-amine, N(4)-bis(aminopropyl)spermidine, was found in thermophilic Thermococcus waiotapuensis, Thermococcus aegaeus and Pyrococcus glycovorans belonging to the order Thermococcales. An acidophilic euryarchaeon, Ferroplasma acidiphilum located in the order Thermoplasmatales, contained spermidine and agmatine. Norspermidine, spermidine, norspermine and spermine were found in thermoacidophilic Acidilobus aceticus and thermophilic Thermodiscus maritimus located in the order Desulfurococcales, and in thermophilic Pyrobaculum arsenaticum, Pyrobaculum oguniense, Vulcanisaeta distributa and Vulcanisaeta souniana belonging to the order Thermoproteales; however, the four genera differ on their tetra- and penta-amine levels. Thermophilic Staphylothermus hellenicus belonging to Desulfurococcales contained caldopentamine, caldohexamine and N1-acetylcaldopentamine in addition to norspermidine, spermidine and norspermine. This is the first report on the occurrence of acetylated penta-amine in nature.
authors.affiliation: Gunma University School of Health Sciences, Showa-machi, Maebashi, Gunma 371-8514, Japan. khamana@health.gunma-u.ac.jp
authors.forename: Koei
authors.initials: K
authors.lastname: Hamana
authors.forename: Takehiko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Hosoya
authors.forename: Masaru
authors.initials: M
authors.lastname: Niitsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019615:Crenarchaeota / Q000737:chemistry* / Q000145:classification; D019605:Euryarchaeota / Q000737:chemistry* / Q000145:classification; D011073:Polyamines / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D011073:Polyamines