title: Stimulation of midgut stem cell proliferation by Manduca sexta alpha-arylphorin.
abstract: Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the observed stem cell proliferation. SDS-PAGE analysis of the protein results in a single band of 77 kDa. Sequences of tryptic peptides from this protein are identical to internal sequences of the storage hexamer alpha-arylphorin. The alpha-arylphorin isolated by our procedure represents a small fraction of the total arylphorin present in the fat body extract. However, it alone seems responsible for the stimulation of mitotic activity in H. virescens midgut stem cells.
authors.affiliation: Insect Biocontrol Laboratory, Plant Sciences Institute, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA. blackbm@ba.ars.usda.gov
authors.forename: Michael B
authors.initials: MB
authors.lastname: Blackburn
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: Loeb
authors.forename: Edward
authors.initials: E
authors.lastname: Clark
authors.forename: Howard
authors.initials: H
authors.lastname: Jaffe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004064:Digestive System / Q000166:cytology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D005216:Fat Body / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D018402:Manduca / Q000166:cytology* / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D006023:Glycoproteins; D019476:Insect Proteins; D010446:Peptide Fragments; C041137:storage proteins, Insecta