title: Characterisation of Hungarian porcine circovirus 2 genomes associated with PMWS and PDNS cases.
abstract: The authors report the data of the first survey on the incidence of post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) in Hungary. A PCR method specific for the detection of porcine circovirus 2 (PCV-2) was developed, which proved to be suitable for diagnostic purposes. PCR screening of organ samples from pigs suspected to be affected with PMWS or PDNS revealed the presence of PCV-2 in 80% of the cases. Six PCV-2 genomes from Hungarian isolates were completely sequenced. Phylogenetic comparison with all the available PCV-2 sequences showed that porcine circoviruses circulating in Hungary are more variable than in several other European countries. Two Hungarian strains clustered together with the Spanish strains forming a distinct group; two others fell in a common group with the French, UK, and Dutch strains, whereas another two strains showed the closest relationship to two of the three known German PCV-2 sequences.
authors.affiliation: Central Veterinary Institute, H-1581 Budapest, PO Box 2, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Dán
authors.forename: T
authors.initials: T
authors.lastname: Molnár
authors.forename: I
authors.initials: I
authors.lastname: Biksi
authors.forename: R
authors.initials: R
authors.lastname: Glávits
authors.forename: M
authors.initials: M
authors.lastname: Shaheim
authors.forename: B
authors.initials: B
authors.lastname: Harrach
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D016678:Genome; D006814:Hungary / Q000453:epidemiology; D015994:Incidence; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*; D019282:Wasting Syndrome / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral