Oocyte transfer in mares with intrauterine or intraoviductal insemination using fresh, cooled, and frozen stallion semen.
The objectives were to compare embryo development rates after oocyte transfer with: (1) intrauterine or intraoviductal inseminations of fresh semen versus intraoviductal insemination of frozen semen; (2) intraoviductal versus intrauterine inseminations of cooled semen. In Experiment I, oocytes were transferred into the oviduct, and recipients were inseminated into the uterus with 1 x 10(9) fresh spermatozoa, or into the oviduct with 2 x 10(5) fresh or frozen-thawed spermatozoa. In Experiment II, semen was cooled to 5 degrees C before intrauterine insemination with 2 x 10(9) spermatozoa or intraoviductal inseminations of 2 x 10(5) spermatozoa (deposited with the oocytes). In Experiment I, embryo development rates were similar (P>0.05) for intrauterine versus intraoviductal inseminations when fresh semen was used (8/14, 57% and 9/11, 82%, respectively). However, embryo development rates were lower (P<0.05) when frozen spermatozoa were placed within the oviduct (1/12, 8%). In Experiment II, embryo development rates were higher (P<0.05) when cooled semen was used for intrauterine (19/23, 83%) versus intraoviductal (4/16, 25%) inseminations. We concluded that intraoviductal insemination can be successfully performed using fresh spermatozoa. However, the use of cooled and frozen spermatozoa for intraoviductal inseminations was less successful, and needs further investigation.
Coutinho da Silva
M A
MA
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA. macoutinho@yahoo.com
Carnevale
E M
EM
Maclellan
L J
LJ
Preis
K A
KA
Seidel
G E
GE
Squires
E L
EL
D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation; D005314:Embryonic and Fetal Development; D005187:Fallopian Tubes; D005260:Female; D006736:Horses; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male; D018587:Oocyte Donation / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D014599:Uterus