HuB localizes to polysomes and alters C/EBP-beta expression in 3T3-L1 adipocytes.
The RNA binding protein HuB was ectopically expressed in 3T3-L1 preadipocytes and localized primarily to the nucleus, as the cells differentiated HuB redistributed to the cytosol and on analysis localized to the dense polysomes. Electron micrographs confirm association of HuB with the ribosomes in the adipocytes consistent with a proposed role in control of translation and mRNA stability. Examination of the expression of C/EBP-beta in the cells expressing HuB relative to the parental 3T3-L1 adipocytes demonstrated an alteration in the LAP to LIP ratio. The data support a role for endogenous Hu proteins in the differentiation process, potentially affecting the rate of differentiation by controlling the concentration of the dominant negative transcription inhibitor, LIP.
Gantt
Kira R
KR
Department of Biochemistry and Molecular Biology, The Brody School of Medicine, Greenville, NC 27858, USA.
Jain
Renu G
RG
Dudek
Ronald W
RW
Pekala
Phillip H
PH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D015153:Blotting, Western; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D005799:Genes, Dominant; D008024:Ligands; D051379:Mice; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D013997:Time Factors; D014162:Transfection
D022782:CCAAT-Enhancer-Binding Protein-beta; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; C086811:ELAVL2 protein, human; D008024:Ligands; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins