title: Cellular requirements for CRM1 import and export.
abstract: We studied the cellular requirements for the translocation of CRM1 (exportin 1) between the nucleus and the cytoplasm. CRM1 import requires neither ATP, Ran, Ran-dependent GTP hydrolysis, nor a particular temperature. CRM1 and importin beta compete with each other during their import. Thus, CRM1 is able to enter the nucleus in a manner similar to importinbeta. In contrast, the in vivo export of CRM1 involves ATP-consuming step(s).
authors.affiliation: Department of Molecular Virology, Institute for Genetic Medicine, Hokkaido University, Hokkaido 060-0815.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Masami
authors.initials: M
authors.lastname: Yamada
authors.forename: Naoto
authors.initials: N
authors.lastname: Mabuchi
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Shida
mesh_terms: D021581:Active Transport, Cell Nucleus; D000255:Adenosine Triphosphate / Q000172:deficiency / Q000378:metabolism; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D028884:Karyopherins / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D013696:Temperature; D028961:beta Karyopherins / Q000378:metabolism; D020931:ran GTP-Binding Protein / Q000378:metabolism; D000097606:Exportin 1 Protein
chemical_list: D000255:Adenosine Triphosphate; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D028961:beta Karyopherins; D020931:ran GTP-Binding Protein; D000097606:Exportin 1 Protein