Chromium (III) enhanced diamine silver staining of proteins and DNA in gels.
Chromium (III) enhanced the sensitivities of diamine silver staining of four proteins between 6- and 50-fold over that of the Coomassie Brilliant Blue (CBB)-chromium modified thiosulfate-silver staining method (Zhou et al. Biotechnology Letters, 2002, 24: 1561-1567). Using six dsDNA fragments, the detection limits of this new method was 10 to 30 pg per band, being 10- to 25-fold more sensitive than previous methods.
Zhou
Zhi-dong
ZD
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Liu
Wang-yi
WY
Li
Min-qian
MQ
D002857:Chromium; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D003959:Diamines; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D003959:Diamines; D011506:Proteins; D012394:Rosaniline Dyes; D002857:Chromium; D012834:Silver; D004247:DNA; C004692:coomassie Brilliant Blue