Assessment of a pyrogallol red technique for total protein measurement in the cerebrospinal fluid of dogs.
The measurement of protein concentration in the cerebrospinal fluid is a basic analytical method in neurology. In this study, a pyrogallol red technique using a human albumin calibrator previously validated in human medicine was tested for canine samples, and the results were compared with those obtained using urine test strips. Pyrogallol red significantly (P<0.05) but moderately underestimated purified dog albumin and globulins. The imprecision of the technique was low: intra- and between-series coefficients of variation were 1.6 and 4.3 per cent at protein concentrations of about 0.3 g/litre. Over 49 samples, there was good agreement between the pyrogallol red and test strip results (r=0.63), especially for low and high protein concentrations, but misclassifications were observed with '+' test strip readings.
Behr
S
S
Département des Sciences Biologiques et Fonctionnelles & UMR Physiopathologie et Toxicologie Expérimentales, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex, France.
Trumel
C
C
Palanché
F
F
Braun
J P
JP
D000818:Animals; D016022:Case-Control Studies; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D004396:Coloring Agents; D004283:Dog Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D004285:Dogs; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D011748:Pyrogallol / Q000031:analogs & derivatives*
D002556:Cerebrospinal Fluid Proteins; D004396:Coloring Agents; D011748:Pyrogallol; C058731:pyrogallol sulfonphthalein