[Effect of fluoroacetamide on cardiomyocytes of rat and the antidotal effect of acetamide].
OBJECTIVE
To observe the effect of fluoroacetamide on cardiomyocytes of rat and the antidotal effect of acetamide.


METHODS
4 groups of SD rats were treated with various dosages of fluoroacetamid(p.o.) and 2 groups of them were treated with acetamide(i.p.). The changes of cardiomyocytes and serum AST, LDH, CK, CK-MB and HBDH were measured at different intervals after poisoning.


RESULTS
In the group treated with fluoroacetamid 8 mg/kg. bw, serum AST[(589.58 +/- 821.72) U/L], CK[(916.78 +/- 343.55) U/L], HBDH[(504.47 +/- 148.88) U/L] raised obviously compared with control[(187.70 +/- 46.87), (755.65 +/- 498.90), (347.25 +/- 228.40) U/L respectively] (P < 0.01), and the pathological findings such as degeneration, liquefactive necrosis and filtration of inflammatory cells in cardiac muscles were observed 24 hours later, while all the male dead within 3 days. In the group treated with fluoroacetamid 4 mg/kg. bw, serum LDH and HBDH rose significantly compared with control(P < 0.01) 5 day later. On the day of 10, myocardial enzymes restored in all experiment groups with some interstitial fibroblastic proliferation. The pathological changes were reduced in the group treated with acetamide synchronously (100 mg/kg. bw).


CONCLUSIONS
Acute intoxication of fluoroacetamide could damage cardiomyocytes while acetamide could reduce the injury of them, but the injury was reversible. The levels of serum myocardial enzymes could be a usable index for early diagnosis.
Zhu
Guanghua
G
Guangdong Provincial Center for Occupational Disease Prevention and Treatment, Guangzhou 510300, China.
Xia
Lihua
L
Lai
Guanzhao
G
Li
Laiyu
L
Huang
Jianxun
J
Tang
Xiaojiang
X
D000081:Acetamides / Q000494:pharmacology*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000931:Antidotes / Q000494:pharmacology*; D052279:Creatine Kinase, MB Form / Q000097:blood; D005463:Fluoroacetates / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000081:Acetamides; D000931:Antidotes; D005463:Fluoroacetates; C030686:acetamide; C007741:fluoroacetamide; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D052279:Creatine Kinase, MB Form