title: Glucose 6-phosphate dehydrogenase is required for sucrose and trehalose to be efficient osmoprotectants in Sinorhizobium meliloti.
abstract: Inactivation of the zwf gene in Sinorhizobium meliloti induces an osmosensitive phenotype and the loss of osmoprotection by trehalose and sucrose, but not by ectoine and glycine betaine. This phenotype is not linked to a defect in the biosynthesis of endogenous solutes. zwf expression is induced by high osmolarity, sucrose and trehalose, but is repressed by betaine. A zwf mutant is more sensitive than its parental strain to superoxide ions, suggesting that glucose 6-phosphate dehydrogenase involvement in the osmotic response most likely results from the production of reactive oxygen species during osmotic stress.
authors.affiliation: Osmorégulation chez les bactéries, CNRS UMR 6026, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
authors.forename: Lise
authors.initials: L
authors.lastname: Barra
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pica
authors.forename: Kamila
authors.initials: K
authors.lastname: Gouffi
authors.forename: Graham C
authors.initials: GC
authors.lastname: Walker
authors.forename: Carlos
authors.initials: C
authors.lastname: Blanco
authors.forename: Annie
authors.initials: A
authors.lastname: Trautwetter
mesh_terms: D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006982:Hypertonic Solutions; D000455:Medicago sativa; D016296:Mutagenesis; D009586:Nitrogen Fixation; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013395:Sucrose / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D006982:Hypertonic Solutions; D013481:Superoxides; D013395:Sucrose; D024483:Vitamin K 3; D014199:Trehalose; D005954:Glucosephosphate Dehydrogenase