title: Isolation of Arcobacter species from animal feces.
abstract: A previously developed Arcobacter isolation protocol for poultry skin and meat was validated for the isolation of Arcobacter from feces of livestock animals. Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. The limit of detection of quantitative and qualitative analysis was 10(2) and 10(0) cfu g(-1) feces, respectively. From fecal samples collected at slaughterhouse, Arcobacter was isolated from 43.9% of porcine, 39.2% of bovine, 16.1% of ovine and 15.4% of equine samples. All three animal-associated Arcobacter species were isolated and levels up to 10(3) cfu g(-1) feces were determined.
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Ellen
authors.initials: E
authors.lastname: van Driessche
authors.forename: Kurt
authors.initials: K
authors.lastname: Houf
authors.forename: Jan
authors.initials: J
authors.lastname: van Hoof
authors.forename: Lieven
authors.initials: L
authors.lastname: De Zutter
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D000003:Abattoirs; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D020640:Arcobacter / Q000302:isolation & purification*; D002417:Cattle; D005243:Feces / Q000382:microbiology*; D006736:Horses; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods / Q000592:standards; D015995:Prevalence; D015203:Reproducibility of Results; D013552:Swine