[Study on the difference in DNA damage of human lymphocyte caused by arsenicals].
OBJECTIVE
To explore whether there is difference in arsenicals-induced DNA damage of human lymphocyte.


METHODS
Lymphocyte were sterilely collected from healthy donor and exposed to sodium arsenite (AsIII), sodium arsenate(AsV) and methyl sodium arsenate(MAsv) at 1,5,10,20 and 50 mumol/L. After incubation of 24 hours, cells were collected by centrifugation and DNA damage was detected by single cell gel electrophoresis (SCGE).


RESULTS
The comet frequency distribution of all groups except 1 mumol/L group of MAsV were significantly different from that of control. The comet length of all groups except 1 mumol/L group of AsV and 1.5 mumol/L groups of MAsV were significantly higher than that of control. There were correlations between the doses of arsenicals and the ratios of comet cell or length of comet(rAsIII = 0.8134, rAsV = 0.8734, rMAsV = 0.8994).


CONCLUSIONS
DNA damage in human lymphocyte were induced by all the three arsenicals. A dose-effect relationship was observed between exposure doses of the same arsenical and DNA damage. With different arsenicals but the same exposure dose, the DNA damage level was as follow: AsIII > AsV > MAsV.
Li
Xin
X
Department of Occupational Health, School of Public Health, China Medical University, Shenyang 110001, China.
Sun
Xiance
X
Sun
Guifan
G
Jin
Yaping
Y
Li
Bing
B
Guo
Xiaoying
X
Liu
Shan
S
D001149:Arsenates / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure
D001149:Arsenates; D018053:Arsenites; C015001:arsenite; C025657:arsenic acid