Frequencies at three polymorphic sites of interleukin-10 gene promoter in Brazilian renal recipients.
We genotyped by PCR-RFLP 141 renal patients (77 men and 64 women with ages between 12 and 58, predominantly Caucasians) for allelic variants of three polymorphic sites of the interleukin-10 gene promoter, previously showed to be associated with different production of IL-10 cytokine: -1082 (G/A), -819 (C/T), and -592 (C/A). These polymorphisms may confer flexibility in the common immune responses and influence the outcome of allo-responses after transplantation. Our aim was to determine the frequencies of three functional polymorphic sites -1082, -819, and -592 of the interleukin-10 gene promoter in renal recipients of Curitiba, Paraná. Paraná State is located in southern Brazil, and its capital is Curitiba with approximately 1.5 million inhabitants. Genotypes were classified as follows: "low" IL-10 producer genotypes (ATA/ATA, ACC/ATA, ACC/ACC), "intermediate" genotypes (GCC/ACC, GCC/ATA), and "high" IL-10 producer genotype (GCC/GCC). In our population we observed linkage disequilibrium between alleles -819C and -592C and this haplotypic combination was more frequent (65%) than -819T and -592A. We found significantly reduced frequency of the genotype and haplotype responsible for high production of interleukin-10, maybe because we have a selected group (only renal patients) or maybe because Brazilian people are very heterogeneous (miscegenational), which may differ from other groups.
Plothow
A
A
Departamento de Genética, Centro Politécnico, Setor de Ciências Biológicas, Universidade Federal do Paraná, Caixa Postal 19071, 81531-990 Curitiba, PR, Brazil.
Benvenutti
R
R
Contieri
F L C
FL
Bicalho
M G
MG
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001938:Brazil; D002648:Child; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016030:Kidney Transplantation / Q000276:immunology*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D044465:White People
D016753:Interleukin-10