title: STK15/Aurora-A expression in primary breast tumors is correlated with nuclear grade but not with prognosis.
abstract: BACKGROUND
  DNA amplification on chromosome 20q13 is commonly detected in breast carcinoma and is correlated with poor prognosis. STK15 maps to this amplicon. The objective of the current study was to use immunohistochemistry to determine STK15 expression in primary breast tumors. The authors also explored whether STK15 was a prognostic factor for breast carcinoma by comparing the level of STK15 gene expression with clinical parameters that are known prognostic factors for the disease.
  
  
  METHODS
  Archival mastectomy and lumpectomy specimens, randomly selected, were immunohistochemically stained to determine the STK15 gene expression level. The clinical parameters of these same patients were reviewed retrospectively and analyzed for correlations with STK15 expression level, based on a positive-versus-negative scoring system.
  
  
  RESULTS
  Of the 112 human breast tumor specimens analyzed, 26% stained positively for STK15 by immunohistochemistry. Of the tumors, that stained positively 62.1% had a well-to-moderately differentiated nuclear grade. The correlation between STK15 staining and nuclear grade was nearly statistically significant (P = 0.05). No association was found between STK15 staining and tumor size, lymph node status, or hormone receptor status. Analysis of recurrence-free survival and overall survival rates also failed to reveal a statistically significant difference between the two groups.
  
  
  CONCLUSIONS
  STK15 expression by immunohistochemistry was noted in approximately one-fourth of primary breast tumors. STK15 expression was associated with nuclear grade, but no correlation was found between the other clinical parameters evaluated. Furthermore, no differences were found in survival rates when they were analyzed by level of STK15 staining.
authors.affiliation: Department of Breast Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA. mroyce@mdanderson.org
authors.forename: Melanie E
authors.initials: ME
authors.lastname: Royce
authors.forename: Weiya
authors.initials: W
authors.lastname: Xia
authors.forename: Aysegul A
authors.initials: AA
authors.lastname: Sahin
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katayama
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Johnston
authors.forename: Gabriel
authors.initials: G
authors.lastname: Hortobagyi
authors.forename: Subrata
authors.initials: S
authors.lastname: Sen
authors.forename: Mien-Chie
authors.initials: MC
authors.lastname: Hung
mesh_terms: D000328:Adult; D000368:Aged; D064096:Aurora Kinase A; D064127:Aurora Kinases; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008408:Mastectomy; D015412:Mastectomy, Segmental; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics*; D012189:Retrospective Studies
chemical_list: C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases