In vitro gene delivery by a novel human calcitonin (hCT)-derived carrier peptide.
Gene therapy still awaits a broader application, since safe and efficient gene delivery is a major problem. Also for the investigation of signal transduction and intracellular trafficking, delivery systems for hydrophilic macromolecules that are easy to use are needed. Several peptide-based delivery systems have been developed during the last years. We present here a novel carrier peptide derived from human calcitonin that is capable of transfecting human neuroblastoma cells by complex formation with a plasmid. Because of the peptide's physiological origin, cytotoxic effects are not expected.
Krauss
Ulrike
U
Institute of Biochemistry, University of Leipzig, Brüderstrasse 34, 04103 Leipzig, Germany.
Müller
Martin
M
Stahl
Michael
M
Beck-Sickinger
Annette G
AG
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002116:Calcitonin / Q000008:administration & dosage* / Q000235:genetics; D045744:Cell Line, Tumor; D006224:Cricetinae; D004337:Drug Carriers / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage* / Q000235:genetics
D004337:Drug Carriers; D010455:Peptides; D002116:Calcitonin