Immunohistochemical and electron-microscopic characteristics of secretory cardiomyocytes in experimental myocardial infarction.
OBJECTIVE
To study immunohistochemical and electron-microscopic features of secretory cardiomyocytes in experimental myocardial infarction (MI).


METHODS
Totally 15 hearts of dogs with experimental MI were studied. For electron-microscopic study materials were collected from left atrium, and its several parts (appendix, lateral and frontal walls). For immunohistochemical analysis of atrial natriuretic factor (ANF) incubation in monoclone immune serum was used (standard monoclonal serum and primary mice antibodies, Immunon). For statistic processing we used Chi-square test (criterion of Pearson agreement).


RESULTS
Immunohistochemical and electron-microscopic investigation after 24 hours from experimental myocardial infarction indicated increase of specific activity of secretory cardiomyocytes and after 48 hours decrease of secretion of ANF while cardiomyopathy appears. After 72 hours, blockade of ANF secretion with decompensation of secretory cardiomyocytes occurred.


CONCLUSIONS
Imunohistochemical investigations and analysis of submicroscopic structures of secretory cardiomyocytes after experimental MI showed, that cells were functionally active 24 hours after myocardial infarction with further (48 hours and 72 hours of MI) decrease in amount and impairement of activity.
Azizov
V A
VA
Department of Internal Medicine, Azerbaijan Medical University, Baku, Azerbaijan.
Muradova
S R
SR
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D004195:Disease Models, Animal; D004285:Dogs; D006325:Heart Atria / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
D009320:Atrial Natriuretic Factor