title: Protein synthesis is necessary for dendritic spine proliferation in adult brain slices.
abstract: Dendritic spines, small protrusions from dendritic shafts, receive most of the excitatory synapses in cortical regions. Spines are highly plastic structures that can be rapidly produced or lost in response to a wide array of internal and external stimuli, and they proliferate in acute slice preparations [J. Neurosci. 19 (1999) 2876]. The goal of the present study was to determine if protein synthesis is necessary for this spine proliferation. We found that the addition of protein synthesis inhibitors to acute slices (in which spines otherwise proliferate) blocked new spine growth. Furthermore, a population of longer spines was observed after 2 h but these did not develop during protein synthesis blockade. These data suggest that protein synthesis is necessary for new spine growth in acute brain slice preparations and support literature suggesting that newly produced spines develop from filopodia-like protrusions.
authors.affiliation: Program in Neuroscience, College of Medicine, Florida State University, Tallahassee, FL 32306-4340, USA. orenda@neuro.fsu.edu
authors.forename: Orenda L
authors.initials: OL
authors.lastname: Johnson
authors.forename: Charles C
authors.initials: CC
authors.lastname: Ouimet
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count; D002455:Cell Division; D048429:Cell Size / Q000187:drug effects / Q000528:radiation effects; D022081:Cell Surface Extensions / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008872:Microwaves; D009473:Neuronal Plasticity / Q000187:drug effects / Q000528:radiation effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
chemical_list: D011500:Protein Synthesis Inhibitors; D011506:Proteins; D011691:Puromycin; D003513:Cycloheximide