title: The -3826 A-->G variant of the uncoupling protein-1 gene diminishes postprandial thermogenesis after a high fat meal in healthy boys.
abstract: This study investigated whether the -3826 A-->G nucleotide variant of the uncoupling protein-1 (UCP1) gene is correlated with postprandial thermogenesis after a high fat meal in children. Healthy boys, aged 8-11 yr, were examined for resting energy expenditure and the thermic effect of a meal (TEM), which were measured by indirect calorimetry for 180 min after a high fat (70% fat, 20% carbohydrate, and 10% protein, providing 30% of the daily energy requirement) and a high carbohydrate meal (20% fat, 70% carbohydrate, and 10% protein). The sympatho-vagal activities were assessed by means of spectral analysis of the heart rate variability during the same period. Children were genotyped for UCP1 polymorphism by applying a PCR-restriction fragment length polymorphism using buccal samples. There was no reaction of sympathetic activity to the high carbohydrate meal in either the GG allele or the AA+AG group and no significant difference in TEM. However, after the high fat meal, sympathetic responses were found in both groups; further, the GG allele group showed significantly lower TEM than the AA+AG group. In conclusion, despite fat-induced sympathetic stimulation, GG allele carriers have a lowered capacity of TEM in response to fat intake, suggesting that such impaired UCP1-linked thermogenesis can have adverse effects on the regulation of body weight.
authors.affiliation: Laboratory of Applied Physiology, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Nagai
authors.forename: Naoki
authors.initials: N
authors.lastname: Sakane
authors.forename: Linda Massako
authors.initials: LM
authors.lastname: Ueno
authors.forename: Taku
authors.initials: T
authors.lastname: Hamada
authors.forename: Toshio
authors.initials: T
authors.lastname: Moritani
mesh_terms: D000225:Adenine; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006147:Guanine; D006801:Humans; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D024101:Mitochondrial Proteins; D011110:Polymorphism, Genetic / Q000235:genetics; D019518:Postprandial Period; D012016:Reference Values; D013564:Sympathetic Nervous System / Q000502:physiology; D022722:Thermogenesis; D013997:Time Factors; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1; D006147:Guanine; D000225:Adenine