title: Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4.
abstract: The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A.
authors.affiliation: Physical Chemistry Division, Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana 10600, Cuba.
authors.forename: Carlos
authors.initials: C
authors.lastname: Martínez-Fleites
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tarbouriech
authors.forename: Miguel
authors.initials: M
authors.lastname: Ortiz-Lombardia
authors.forename: Edward
authors.initials: E
authors.lastname: Taylor
authors.forename: Armando
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ramírez
authors.forename: Lázaro
authors.initials: L
authors.lastname: Hernández
authors.forename: Gideon J
authors.initials: GJ
authors.lastname: Davies
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D041821:Gluconacetobacter / Q000201:enzymology*; D006602:Hexosyltransferases / Q000737:chemistry*
chemical_list: D006602:Hexosyltransferases; C021513:levansucrase