A rapid and specific whole blood HPA-1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb-IIIa complexes.
The human platelet antigen 1 (HPA-1) system has been implicated in rare but severe diseases, such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and immune platelet refractoriness. We developed a flow cytometry assay for HPA-1 phenotyping using two commercial monoclonal antibodies, P2 and SZ21, directed against glycoprotein (GP) IIb-IIIa and GP IIIa respectively. One hundred and twenty-seven healthy controls were studied and ratios of mean fluorescence intensity for P2 and SZ21 discriminated between HPA-1a homozygotes and heterozygotes. These two monoclonal antibodies, coupled with flow cytometry represent a rapid and reliable tool for platelet HPA-1 typing to aid diagnosis.
Sorel
Nathalie
N
Laboratoire d'hématologie et des maladies du sang, CHU de Poitiers, hôpital La Miletrie, Poitiers 86021, France. n.sorel@chu-poitiers.fr
Brabant
Severine
S
Christiaens
Luc
L
Brizard
Andre
A
Mauco
Gerard
G
Macchi
Laurent
L
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D039661:Integrin beta3 / Q000276:immunology*; D010641:Phenotype; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C083451:human platelet antigen 1b