Proteome profiling and its use in metabolic and cellular engineering.
Proteome profiling of microorganisms makes it possible to generate valuable knowledge that can be used for the development of metabolic and cellular engineering strategies, which consequently are used to enhance the yield and productivity of native or foreign bioproducts and to modify cellular properties to improve mid-stream and down-stream processes. Advances in two-dimensional gel electrophoresis technology combined with mass spectrometry allow the creation of global scale proteome contents which can be used to elucidate valuable information on the dynamics of the metabolic, signaling and regulatory networks apart from understanding the physiological changes. In this paper, we review the approaches of exploiting the proteome profiling results to the development of the strategies for the metabolic and cellular engineering of microorganisms.
Han
Mee-Jung
MJ
Metabolic and Biomolecular Engineering National Research Laboratory, Korean Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Lee
Sang Yup
SY
D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011108:Polymers; D020543:Proteome; D011994:Recombinant Proteins; C111948:poly(hydroxyethanoate)