Capillary electrophoresis method for simultaneous determination of penicillin G, procaine and dihydrostreptomycin in veterinary drugs.
Capillary electrophoresis method for identification and simultaneous determination of procaine, dihydrostreptomycin and penicillin G, present in multiantibiotic veterinary preparations, was elaborated. The influence of pH (5.0-9.75) and concentration of disodium tetraborate decahydrate in running buffers (0.02-0.1 M) as well as temperatures (25-40 degrees C) on separation efficacy were analyzed. For quantitative analysis, 0.08 M borate buffer (pH 8.0) at 35 degrees C and 15 kV were chosen. Method was validated, selectivity, precision, linearity, LOD, LOQ, accuracy and specificity of capillary zone electrophoresis (CZE) were evaluated.
Michalska
Katarzyna
K
Antibiotics and Microbiology Department, National Institute of Public Health, 30-34 Chelmska Street, 00-725 Warsaw, Poland. tyski@il.waw.pl
Pajchel
Genowefa
G
Tyski
Stefan
S
D002021:Buffers; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis*; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D010402:Penicillin G Procaine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D019155:Veterinary Drugs / Q000032:analysis
D002021:Buffers; D007202:Indicators and Reagents; D019155:Veterinary Drugs; D010402:Penicillin G Procaine; D004096:Dihydrostreptomycin Sulfate