title: Plastic substrates based separation channels in electromigration techniques.
abstract: Three types of plastic materials (polyester, polyurethane and polymethylmethacrylate) were tested as materials for manufacturing separation columns (polyester and polyurethane capillaries were used) or separation channels (polymethylmethacrylate) in the chip format. A set of 11 fluorescein isothiocyanate amino acid derivatives was used as the test mixture. Using alpha-cyclodextrin additive to the background electrolyte in the case of the chip separation was also tested. The main problem with all plastic separation media was the selectivity of the separation. The best results, practically identical with bare fused silica capillary, were obtained with the polymethylmethacrylate chip, provided that alpha-cyclodextrin in a concentration 40 mmol/l was added to the background electrolyte. An important observation was that in SDS containing background electrolyte all the plastic materials used exhibited a distinct electroosmotic flow, which was ascribe to the sorption of the negatively charged constituents of the background electrolyte to the capillary wall. Regarding the order in which the individual components of the test mixture were brought to the detector only a single change was observed. Histidine migrated in the polystyrene and polymethylmethacrylate separation channels more slowly than in the bare silica or polyurethane based capillaries.
authors.affiliation: Institute of Physiology, Academy of Science of the Czech Republic, Vídenská 1083, 14220 Prague 4, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Charvátová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Deyl
authors.forename: Miroslav
authors.initials: M
authors.lastname: Klevar
authors.forename: Ivan
authors.initials: I
authors.lastname: Miksík
authors.forename: Adam
authors.initials: A
authors.lastname: Eckhardt
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007202:Indicators and Reagents; D008838:Microcomputers; D010969:Plastics / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D012015:Reference Standards; D012822:Silicon Dioxide
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010969:Plastics; D011091:Polyesters; D011140:Polyurethanes; D012822:Silicon Dioxide; D019904:Polymethyl Methacrylate; D016650:Fluorescein-5-isothiocyanate