title: Effect of lipopolysaccharide infusion on serum macromineral and vitamin D concentrations in dairy cows.
abstract: Four multiparous lactating cows (175 to 220 d in milk) were used in a 4 x 4 Latin square design to assess the effects of four doses (0.0, 0.5, 1.0, 1.5 microg/kg of body weight) of lipopolysaccharide (LPS; Escherichia coli 0111:B4) on circulating concentrations of macrominerals and vitamin D metabolites. Treatments were dissolved in 100 ml of sterile saline and infused intravenously over a period of 100 min. Blood was sampled immediately before infusion (0 h), at 60-min intervals for 8 h, and at 24 and 48 h postinfusion. Vitamin D metabolites were analyzed in samples collected at 0, 2, 6, 24, and 48 h only. Serum Ca and P concentrations decreased after LPS infusion, but there was no effect on serum magnesium concentration. Plasma 25-OH vitamin D3 and 1,25-(OH)2 vitamin D3 were not affected by LPS infusion; however, when analyzed as 0 vs. all other doses of LPS combined, there was a tendency for plasma 1,25-(OH)2 vitamin D3 concentration to decrease when cows were infused with LPS. The inflammatory response elicited by LPS altered plasma macromineral concentrations, a result that may have important implications for calcium homeostasis and metabolic health of lactating dairy cows.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca 14853, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Waldron
authors.forename: B J
authors.initials: BJ
authors.lastname: Nonnecke
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: R L
authors.initials: RL
authors.lastname: Horst
authors.forename: T R
authors.initials: TR
authors.lastname: Overton
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000097:blood; D002417:Cattle / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D002762:Cholecalciferol / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D007262:Infusions, Intravenous / Q000662:veterinary; D007700:Kinetics; D007774:Lactation / Q000097:blood / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008274:Magnesium / Q000097:blood; D008903:Minerals / Q000097:blood*; D010758:Phosphorus / Q000097:blood; D011897:Random Allocation; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood*
chemical_list: D008070:Lipopolysaccharides; D008903:Minerals; C440499:lipopolysaccharide, Escherichia coli O111 B4; D014807:Vitamin D; D002762:Cholecalciferol; D010758:Phosphorus; D002117:Calcitriol; D008274:Magnesium; D002118:Calcium