A domain-specific usherin/collagen IV interaction may be required for stable integration into the basement membrane superstructure.
Usherin is a basement membrane protein encoded by the gene associated with Usher syndrome type IIa, the most common deaf/blind disorder. This report demonstrates a specific interaction between type IV collagen and usherin in the basement membrane, with a 1:1 stoichiometry for binding. Genetic and biochemical approaches were used to explore the role of type IV collagen binding in usherin function. We demonstrate binding occurs between the LE domain of usherin and the 7S domain of type IV collagen. A purified fusion peptide comprising the first four LE modules was shown to compete with full-length recombinant usherin for type IV collagen binding. However, synonymous fusion peptides with single amino acid substitutions resulting from missense mutations that were known to cause Usher syndrome type IIa in humans, failed to compete. Only mutations in loop b of the LE domain abolished binding activity. Co-immunoprecipitation and western blot analysis of testicular basement membranes from the Alport mouse model show a 70% reduction in type IV collagen is associated with a similar reduction in usherin, suggesting the usherin/collagen (IV) interaction stabilizes usherin in the basement membrane. Thus, the domain-specific interaction between usherin and type IV collagen appears essential to usherin stability in vivo, and loss of this interaction may result in Usher pathology in humans.
Bhattacharya
Gautam
G
Usher Syndrome Center, Boys Town National Research Hospital, 555 No. 30th Street, Omaha, Nebraska, 68131, USA.
Kalluri
Raghu
R
Orten
Dana J
DJ
Kimberling
William J
WJ
Cosgrove
Dominic
D
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D016253:Microscopy, Immunoelectron; D008961:Models, Structural; D009154:Mutation; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020349:Surface Plasmon Resonance
D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; C112735:USH2A protein, human; C491810:Ush2a protein, mouse