An optimization of isolation of early hematopoietic cells from heparinized cadaveric organ donors.
Heparinized cadaveric organ donors are a potential source of hematopoietic cells for transplantation purposes. The aim of this study was to optimize the harvest of early hematopoietic cells from cadaver donors. Accordingly, we noticed that bone marrow cells harvested from cadaver donors should be resuspended in RPMI or Iscove's medium supplemented with heparin or ACD. Bags with harvested marrow should contain 20% atmosphere air during short-term storage/transportation. Finally, we noticed that BMMNC survive short-term storage better if the collected marrow is not depleted of erythrocytes.
Machaliński
B
B
Department of General Pathology, Pomeranian Medical University, Al Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Paczkowski
M
M
Kawa
M
M
Paczkowska
E
E
Ostrowski
M
M
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D001926:Brain Death; D002102:Cadaver; D002423:Cause of Death; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods
D000925:Anticoagulants; D003470:Culture Media; D006493:Heparin; D004492:Edetic Acid