[The relationship between IL-13 gene polymorphism and the levels of serum IL-13 and serum eosinophil cation protein in asthmatic children].
OBJECTIVE
To explore the relationship between IL-3 gene polymorphism and the levels of serum IL-3 and eosinophil cation protein (ECP) for understanding the role of IL-3 gene polymorphism in the mechanism of childhood asthma.


METHODS
The method of restriction fragment length polymorphism (RFLP) was adopted in detecting +1923 site polymorphism of IL-13 gene in intron 3 region, ELISA was employed in detecting the level of serum IL-13, and fluorescent enzyme-linked immunoassay was used to detect the level of serum ECP.


RESULTS
The frequency distribution of TT, TC genotypes of IL-13 Intron 3+1923 site in asthmatic children was higher than that of CC genotype in normal control (P<0.05), and the levels of serum IL-13 and ECP of TT, TC genotypes were significantly higher than those of CC genotype respectively (P<0.01).


CONCLUSIONS
The close relationship of IL-3 gene polymorphism with the levels of serum IL-13 and ECP suggests that IL-3 gene polymorphism may play an important role in the mechanism of childhood asthma.
Sun
Hai-ping
HP
Huai'an Maternal and Children Health Hospital, Huai'an, Jiangsu, 223002 PR China. hasunhp@tom.com
Chen
Jie-qing
JQ
Guo
Xi-rong
XR
Chen
Rong-hua
RH
D001249:Asthma / Q000097:blood / Q000235:genetics*; D001798:Blood Proteins; D002648:Child; D002675:Child, Preschool; D047091:Eosinophil Granule Proteins; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D018793:Interleukin-13 / Q000097:blood / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D012260:Ribonucleases / Q000097:blood*
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018793:Interleukin-13; D012260:Ribonucleases