Distribution analysis of IS231-like sequences among Bacillus thuringiensis serovars inferred from restriction fragment length polymorphisms.
The distribution of IS231 has been analyzed in Bacillus thuringiensis serovars. A 723-bp HaeII conserved fragment from IS231M has been used as a probe against EcoRI-digested B. thuringiensis total DNA to yield serovar-specific hybridization profiles. The approach was useful at revealing the extent of distribution of IS231-like sequences between and within strains. Of the 88 B. thuringiensis strains tested, 70 showed hybridization banding patterns that comprised between one and 20 distinct bands. These 70 B. thuringiensis strains were grouped based on banding pattern similarities. Interestingly, intraserovar strains did not necessarily cluster together.
Joung
Kwang-Bo
KB
Agriculture and Agri-Food Canada, Research Centre, 430 Gouin Blvd., Saint-Jean-sur-Richelieu, Québec, Canada, J3B 3E6.
Côté
Jean-Charles
JC
D001413:Bacillus thuringiensis / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics / Q000302:isolation & purification; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial