title: Establishment of cell lines from a primary hepatocellular carcinoma and its metastatis.
abstract: Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world with a very poor prognosis that has been associated with tumor metastasis. The molecular mechanism of HCC metastasis is still unclear. In this study, we established cell lines from a primary tumor (H2-P) and its metastasis (H2-M). G-banding karyotyping, comparative genomic hybridization, and fluorescence in situ hybridization were applied to study these two cell lines and the results demonstrated that they are of the same origin. These cell lines provide a very useful tool to identify genetic alterations associated with HCC metastasis.
authors.affiliation: Department of Clinical Oncology, The University of Hong Kong, Room 109, 1/F, School of Chinese Medicine Building, 10 Sassoon Road, Hong Kong, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Wen
authors.forename: Jonathan S T
authors.initials: JS
authors.lastname: Sham
authors.forename: Weisheng
authors.initials: W
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Wai Mui
authors.initials: WM
authors.lastname: Tjia
authors.forename: Jie-Fu
authors.initials: JF
authors.lastname: Huang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Wei-Fen
authors.initials: WF
authors.lastname: Zeng
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Guan
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology* / Q000556:secondary; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured