Ethnic variation of the P53 codon 72 polymorphism, HPV persistence, and cervical cancer risk.
Association between the p53 codon 72 polymorphism and cervical cancer remains unresolved. We determined the association between the polymorphism and risk of human papillomavirus (HPV) persistence. The polymorphism was detected by restriction enzyme digestion following p53 amplification and HPV detection by the PGMY 09/11 primer set followed by reverse line blot hybridization: 3371 samples were analysed. HPV persistence was assessed on a subset of samples collected at baseline, four and 10 months (n =442). Highly significant differences were observed between ethnic groups (P <0.005). No associations were found between P53 arginine and cytological grade in women infected with any HPV or any oncogenic HPV, despite adjustment for ethnicity. These results were sustained even when HPV-negative women were used as controls. Persistence for any or oncogenic HPV infection was not associated with the polymorphism, irrespective or ethnicity adjustment. Our findings do not support a role for this polymorphism conferring elevated risk for HPV-related disease.
Inserra
P
P
University of Arizona Cancer Center, Tucson, AZ 85724, USA. Pinser@dcc.edu
Abrahamsen
M
M
Papenfuss
M
M
Giuliano
A R
AR
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000208:ethnology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014412:Tumor Virus Infections / Q000208:ethnology / Q000235:genetics; D014481:United States; D002583:Uterine Cervical Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000821:virology
D003062:Codon; D016159:Tumor Suppressor Protein p53