title: Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD.
abstract: Two general pathways for cell death have been defined, apoptosis and necrosis. Previous studies in Jurkat cells have demonstrated that the Fas-associated death domain (FADD) is required for Fas-mediated signaling to apoptosis and necrosis. Here we developed L929rTA cell lines that allow Tet-on inducible expression and FK506-binding protein (FKBP)-mediated dimerization of FADD, FADD-death effector domain (FADD-DED), or FADD-death domain (FADD-DD). We show that expression and dimerization of FADD leads to necrosis. However, pretreatment of the cells with the Hsp90 inhibitor geldanamycin, which leads to proteasome-mediated degradation of receptor interacting protein 1 (RIP1), reverts FKBP-FADD-induced necrosis to apoptosis. Expression and dimerization of FADD-DD mediates necrotic cell death. We found that FADD-DD is able to bind RIP1, another protein necessary for Fas-mediated necrosis. Expression and dimerization of FADD-DED initiates apoptosis. Remarkably, in the presence of caspase inhibitors, FADD-DED mediates necrotic cell death. Coimmunoprecipitation studies revealed that FADD-DED in the absence procaspase-8 C/A is also capable of recruiting RIP1. However, when procaspase-8 C/A and RIP1 are expressed simultaneously, FADD-DED preferentially recruits procaspase-8 C/A.
authors.affiliation: Molecular Signalling and Cell Death Unit, Department of Molecular Biomedical Research, the Flanders Interuniversity Institute for Biotechnology (VIB) and Gent University, Technologiepark 927, B-9052 Zwijnaarde, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Vanden Berghe
authors.forename: Geert
authors.initials: G
authors.lastname: van Loo
authors.forename: Xavier
authors.initials: X
authors.lastname: Saelens
authors.forename: Maria
authors.initials: M
authors.lastname: Van Gurp
authors.forename: Greet
authors.initials: G
authors.lastname: Brouckaert
authors.forename: Michael
authors.initials: M
authors.lastname: Kalai
authors.forename: Wim
authors.initials: W
authors.lastname: Declercq
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D053181:Caspase 8; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D019281:Dimerization; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004792:Enzyme Precursors / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein; D005354:Fibrosarcoma; D005434:Flow Cytometry; D015870:Gene Expression; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors; D006801:Humans; D051379:Mice; D009336:Necrosis; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D011506:Proteins / Q000378:metabolism; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D011994:Recombinant Proteins; D015398:Signal Transduction / Q000502:physiology*; D022021:Tacrolimus Binding Proteins / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D004792:Enzyme Precursors; C505533:FADD protein, human; C505534:Fadd protein, mouse; D053200:Fas-Associated Death Domain Protein; D018841:HSP90 Heat-Shock Proteins; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C506249:RIPK1 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; C506251:Ripk1 protein, mouse; C505530:CASP8 protein, human; C505526:Casp8 protein, mouse; D053181:Caspase 8; D020169:Caspases; D022021:Tacrolimus Binding Proteins