Effect of UV-C mediated oxidative stress in leukemia cell lines and its relation to ubiquinone content.
UV-C radiation is able to impair cellular functions by directly damaging DNA, and by inducing an increased formation of reactive oxygen species that leads to a condition of oxidative stress. In this study we evaluated different responses to UV insult of two leukemia cell lines, HL-60 and Raji, and the relationship with their CoQ10 content. DNA damage was monitored by means of the alkaline single cell gel electrophoresis (Comet assay); intracellular levels of ROS, mitochondrial depolarization and cell viability was measured by flow cytometry. Raji cells appeared more resistant to the UV insult; moreover, they did not show any increase in ROS content and the extent of mitochondrial depolarisation was much lower than in HL 60 cell line. Raji cells also contained significantly higher levels of CoQ10 and their ability to incorporate and to reduce exogenous CoQ10 added to the culture medium was remarkably elevated compared with HL 60.
Brugè
F
F
Institute of Biochemistry, Università Politecnica delle Marche Medical School, Via Ranieri, Montedago 60131, Ancona, Italy.
Tiano
L
L
Cacciamani
T
T
Principi
F
F
Littarru
G P
GP
D002470:Cell Survival; D003067:Coenzymes; D004249:DNA Damage / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008564:Membrane Potentials; D008928:Mitochondria / Q000648:ultrastructure; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011419:Propidium; D017382:Reactive Oxygen Species / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D014466:Ultraviolet Rays
D003067:Coenzymes; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D011419:Propidium; C024989:coenzyme Q10