Determination of bisphenol A and 4-nonylphenol in human milk using alkaline digestion and cleanup by solid-phase extraction.
A highly sensitive and selective method based on alkaline digestion for the simultaneous determination of bisphenol A (BPA) and 4-nonylphenol (NP) was developed. The method consists of digestion of the matrix with ethanolic KOH, extraction with diethyl ether under a mild alkaline condition, cleaning with successive aminopropyl (NH2) cartridges and derivatization followed by a GC-MS analysis. The assay accuracies, expressed as recoveries, were 82 - 113% for BPA and 89 - 97% for NP. The limits of detection of BPA and NP were 0.09 ng/g and 0.50 ng/g, respectively. The procedure will be reliable for the trace analysis of BPA and NP in human milk, since alkaline digestion can diminish their documented association with protein.
Otaka
Hiroaki
H
National Environmental Research and Training Institute, Ministry of the Environment, 3-3 Namiki, Tokorozawa, Saitama 359-0042, Japan. hiroaki_otaka@env.go.jp
Yasuhara
Akio
A
Morita
Masatoshi
M
D000468:Alkalies; D001559:Benzhydryl Compounds; D005591:Chemical Fractionation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008895:Milk, Human / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D000468:Alkalies; D001559:Benzhydryl Compounds; D010636:Phenols; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds