Multiharmonic-generation biopsy of skin.
Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.
Sun
Chi-Kuang
CK
Department of Electrical Engineering and Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei 10617, Taiwan. sun@cc.ee.ntu.edu.tw
Chen
Cheng-Chi
CC
Chu
Shi-Wei
SW
Tsai
Tsung-Han
TH
Chen
Yung-Chih
YC
Lin
Bai-Ling
BL
D000818:Animals; D001706:Biopsy / Q000379:methods*; D020405:Dermis / Q000473:pathology; D004423:Ear / Q000473:pathology; D007834:Lasers; D051379:Mice; D017785:Photons; D012867:Skin / Q000473:pathology*