Development of a monoclonal antibody capable of detecting prolamine in wheat and oats.
A monoclonal antibody (MAb) capable of detecting the gliadin in wheat flour has been developed. By immunoblot, the monoclonal antibody recognized three bands in the membranes after electrophoresis in an SDS PAGE of the alcohol-soluble proteins of wheat, and only one band in the alcohol extract of the oat flour. ELISA technique can be applied using this monoclonal antibody in the detection of gliadin and avenin in samples from wheat or triticale. The characterization of the antibody shows that it is an IgM class. Sensitivity by ELISA to the gliadin was 80 ng/ml.
Gil García
J R
JR
Instituto de Biotecnología, Facultad de Ciencias, Campus Fuentenueva, Universidad de Granada, 18071 Granada, Spain.
Solano
Jennifer
J
Brazil-Dos Santos
F
F
Osuna
A
A
D000438:Alcohols / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D018554:Avena / Q000737:chemistry*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000737:chemistry; D006825:Hybridomas / Q000378:metabolism; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010665:Phenylpropanolamine / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry; D055315:Prolamins; D012680:Sensitivity and Specificity; D013997:Time Factors; D014908:Triticum / Q000737:chemistry
D000438:Alcohols; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D010940:Plant Proteins; D055315:Prolamins; D010665:Phenylpropanolamine; D005903:Gliadin