title: Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis.
abstract: Messenger RNA polyadenylation is one of the processes that control gene expression in all eukaryotic cells and tissues. In mice, two forms of the regulatory polyadenylation protein CstF-64 are found. The gene Cstf2 on the X chromosome encodes this form, and it is expressed in all somatic tissues. The second form, tauCstF-64 (encoded by the autosomal gene Cstf2t), is expressed in a more limited set of tissues and cell types, largely in meiotic and postmeiotic male germ cells and, to a smaller extent, in brain. We report here that whereas CstF-64 and tauCstF-64 expression in rat tissues resembles their expression in mouse tissues, significant differences also are found. First, unlike in mice, in which CstF-64 was expressed in postmeiotic round and elongating spermatids, rat CstF-64 was absent in those cell types. Second, unlike in mice, tauCstF-64 was expressed at significant levels in rat liver. These differences in expression suggest interesting differences in X-chromosomal gene expression between these two rodent species.
authors.affiliation: Department of Cell Biology & Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.
authors.forename: A Michelle
authors.initials: AM
authors.lastname: Wallace
authors.forename: Toni L
authors.initials: TL
authors.lastname: Denison
authors.forename: Ebtesam N
authors.initials: EN
authors.lastname: Attaya
authors.forename: Clinton C
authors.initials: CC
authors.lastname: MacDonald
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D039241:Cleavage Stimulation Factor / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D008297:Male; D008540:Meiosis; D051379:Mice / Q000378:metabolism*; D008815:Mice, Inbred Strains; D051381:Rats / Q000378:metabolism*; D017208:Rats, Wistar; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D039241:Cleavage Stimulation Factor; C492659:Cstf1 protein, rat; C492658:Cstf2t protein, mouse