[Effect of allitridi on cyclin D1 and p27(Kip1) protein expression in gastric carcinoma BGC823 cells].
OBJECTIVE
Previous study showed that allitridi markedly suppresses cellular proliferation, blocks cell cycle at G(1) phase, and induces cell apoptosis of human gastric cancer BGC823 cell. During the investigation of its molecular mechanisms and target genes, the authors found that protein expression of cyclin D1 and p27(Kip1) play an important role in an allitridi-induced G(1) arrest. This study was designed to explore the effect of allitridi on the expression of cyclin D1 and p27(Kip1) in BGC823 cells.


METHODS
After total RNA and total protein in allitridi-treated (25 microg/ml) and allitridi-untreated BGC823 cells were abstained, Western blot analysis was performed to determine the protein levels of cyclin D1 and p27(Kip1), and RT-PCR was performed to determine the mRNA levels of cyclin D1 and p27(Kip1).


RESULTS
After treated with 25 microg/ml allitridi for 24 hours, the protein levels of cyclin D1 of BGC823 cells were downregulated and the protein levels of p27(Kip1) were upregulated, and this changes were more obvious after 48 hours,while their mRNA levels remained unchanged.


CONCLUSIONS
Allitridi may influence the protein expression of cyclin D1 and p27(Kip1) in BGC823 cells, while do not influence their mRNA transcription.
Lan
Hong
H
Beijing Laboratory of Molecular Oncology, Beijing Institute for Cancer Research, School of Oncology, Peking University, Beijing, PR China.
Lu
You-Yong
YY
D000498:Allyl Compounds / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D050760:Cyclin-Dependent Kinase Inhibitor p27; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
D000498:Allyl Compounds; D000975:Antioxidants; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D013440:Sulfides; D025521:Tumor Suppressor Proteins; C042577:diallyl trisulfide; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27