title: Effects of rhinovirus infection on the adherence of Streptococcus pneumoniae to cultured human airway epithelial cells.
abstract: To examine the effects of rhinovirus (RV) infection on the adherence of Streptococcus pneumoniae to human tracheal epithelial cells, cells were infected with RV-14, and S. pneumoniae were added to the culture medium. The number of S. pneumoniae adhering to epithelial cells increased after RV infection. Y-24180, a specific inhibitor of the platelet-activating factor receptor (PAF-R); PAF; and the pyrrolidine derivative of dithiocarbamate, an inhibitor of transcription factor nuclear factor-kappaB (NF-kappaB), decreased the number of S. pneumoniae adhering to cells after RV-14 infection. RV-14 infection increased PAF-R expression and the activation of NF-kappaB and promoter-specific transcription factor 1. These findings suggest that RV-14 infection stimulates S. pneumoniae adhesion to airway epithelial cells via increases in PAF-Rs that are partly mediated through activation of transcription factors. Increased adherence of S. pneumoniae may be one of the reasons that pneumonia develops after RV infection.
authors.affiliation: Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishizuka
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Yamaya
authors.forename: Tomoko
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Takahashi
authors.forename: Shiroh
authors.initials: S
authors.lastname: Ida
authors.forename: Takahiko
authors.initials: T
authors.lastname: Sasaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Inoue
authors.forename: Kiyohisa
authors.initials: K
authors.lastname: Sekizawa
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Nishimura
authors.forename: Hidetada
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology; D010850:Picornaviridae Infections; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D011008:Pneumococcal Infections / Q000382:microbiology*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism / Q000382:microbiology; D012229:Rhinovirus / Q000472:pathogenicity*; D013296:Streptococcus pneumoniae / Q000472:pathogenicity / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology; D014774:Virulence
chemical_list: D016328:NF-kappa B; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D014157:Transcription Factors; C039351:platelet activating factor receptor