Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes.
BACKGROUND
Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes.


METHODS
Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects.


RESULTS
This ELISA has high specificity and sensitivity over the concentration of range 0.5-100 ng/ml with good percentage recovery (97.1 +/- 4.7%) and reproducibility (within-day assay, CV = 4.8-8.6%; between-day assay, CV = 5.6-9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean +/- S.E.: 20.8 +/- 0.7 vs. 14.9 +/- 0.5 ng/ml, p < 0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects (r = 0.412, p < 0.0003) and patients with type 2 diabetes (r = 0.395, p < 0.0001).


CONCLUSIONS
Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans.
Fujinami
Aya
A
Department of Clinical Chemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan.
Obayashi
Hiroshi
H
Ohta
Kiyoe
K
Ichimura
Toshiko
T
Nishimura
Masataka
M
Matsui
Hidehito
H
Kawahara
Yuji
Y
Yamazaki
Masahiro
M
Ogata
Masakazu
M
Hasegawa
Goji
G
Nakamura
Naoto
N
Yoshikawa
Toshikazu
T
Nakano
Koji
K
Ohta
Mitsuhiro
M
D000273:Adipose Tissue / Q000378:metabolism; D000906:Antibodies / Q000276:immunology; D001835:Body Weight; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006729:Hormones, Ectopic / Q000097:blood* / Q000276:immunology; D006801:Humans; D052243:Resistin
D000906:Antibodies; D006729:Hormones, Ectopic; C499624:RETN protein, human; D052243:Resistin