title: Comparative analysis on the 5'-untranslated region of bovine viral diarrhea virus isolated in Korea.
abstract: Samples of 249 bovine abortuses, one intestine, and four diarrheal stools from 254 cows were collected, and bovine viral diarrhea virus (BVDV) was detected by reverse transcription-polymerase chain reaction (RT-PCR). Subsequently, virus isolation was preformed with PCR-positive samples, and then PCR product of 5(')-untranslated region (UTR) of BVDV isolate was sequenced and analyzed. Among the samples collected, 20 (from 17 bovine abortuses, one intestine, and two diarrheal stools) were positive for BVDV RT-PCR; four BVDVs (from two bovine abortuses, one intestine, and one diarrheal stool) were isolated. When the four isolates were biotyped in cell culture, one BVDV isolate from a bovine abortus was cytopathic and the others were non-cytopathic. In addition, three isolates were genotyped as BVDV-1 and one isolate from a diarrheal stool as BVDV-2. In phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea.
authors.affiliation: Department of Veterinary Medicine Virology Lab, The Xenotransplantation Research Center, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, 151-742, Seoul, Republic of Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: H J
authors.initials: HJ
authors.lastname: Moon
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
authors.forename: W S
authors.initials: WS
authors.lastname: Hwang
authors.forename: H S
authors.initials: HS
authors.lastname: Yoo
authors.forename: D Y
authors.initials: DY
authors.lastname: Kim
authors.forename: B K
authors.initials: BK
authors.lastname: Park
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D007723:Korea; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral