title: Hierarchical chromatin structure of Schizosaccharomyces pombe revealed by atomic force microscopy.
abstract: Many structural studies on higher eukaryotic chromatin have been carried out, but chromatin structure in fungi remains unclear. Schizosaccharomyces pombe has been used for investigations of chromosome function; however, the structural details of S. pombe chromatin have not been clarified owing to its small nucleus. We used atomic force microscopy for nano-scale imaging of chromatin isolated from S. pombe. Topographic images indicated that nuclear chromatin contained at least three hierarchical structures: large-scale chromatin fibers, spherical domains in the fibers, and nodules in the domains. The average diameters of the domain and the nodule were 363 +/- 85.2 nm and 46.2 +/- 9.30 nm. Each structure comprising the hierarchy was similar to higher eukaryotic chromatin thus far observed, despite definite differences in chromatin organization at the nucleosomal level. The presence of histone H1 suggested that there might be an alternative to compensate for histone H1 lacking in S. pombe.
authors.affiliation: Food Engineering Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba 305-8642, Japan. tkobo@affrc.go.jp
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kobori
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yoshino
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Ohtani
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D002843:Chromatin / Q000302:isolation & purification / Q000648:ultrastructure*; D006657:Histones / Q000235:genetics / Q000502:physiology; D018625:Microscopy, Atomic Force; D012568:Schizosaccharomyces / Q000648:ultrastructure*
chemical_list: D002843:Chromatin; D006657:Histones