QTL analysis of variation in male courtship song characters in Drosophila virilis.
We have used a quantitative trait locus (QTL) mapping approach to study the genetic basis of differences between two Drosophila virilis strains representing extreme phenotypes in two song characters, the number of pulses in a pulse train (PN) and the length of a pulse train (PTL). Variation in these characters among 520 F2 males was studied by single-marker analysis and composite interval mapping (CIM) using a recombination linkage map constructed for 26 microsatellite markers. In single-marker analysis, two adjacent microsatellite markers on the third chromosome, msat19 and vir84 explained 13.8 and 12.4% of the variation in PN and 9.9 and 6.5% of the variation in PTL, respectively. CIM analysis revealed significant QTLs affecting PN, located on the X and the second, third and fourth chromosome of D. virilis, while variation in PTL was attributable to QTLs located only on the third chromosome.
Huttunen
S
S
Department of Biology, University of Oulu, Finland. susanna.huttunen@oulu.fi
Aspi
J
J
Hoikkala
A
A
Schlötterer
C
C
D000818:Animals; D002874:Chromosome Mapping; D003380:Courtship; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D017344:Genes, Insect; D005819:Genetic Markers; D014644:Genetic Variation; D008297:Male; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D014828:Vocalization, Animal / Q000502:physiology*
D005819:Genetic Markers