Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors.
Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.
Lucarelli
Fausto
F
Dipartimento di Chimica, Universita' degli Studi di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, Firenze, Italy. fausto.lucarelli@unifi.it
Marrazza
Giovanna
G
Turner
Anthony P F
AP
Mascini
Marco
M
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000138:chemical synthesis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D006046:Gold; D006801:Humans; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D014159:Transducers
D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D002244:Carbon; D006046:Gold; D004247:DNA