Integrins on eggs: the betaC subunit is essential for formation of the cortical actin cytoskeleton in sea urchin eggs.
Eggs of several metazoans have been demonstrated to express integrins; however, their function is unclear. Previous studies have shown that the betaC integrin subunit is expressed on unfertilized sea urchin eggs and proteolytically removed at fertilization. Here we report that the betaC subunit is reexpressed on the egg surface immediately after fertilization. Using morpholino antisense oligonucleotides to block translation, we show that without betaC expression, eggs undergo cleavage resulting in loosely adherent cells that fail to develop beyond a blastula. Without betaC containing integrins, the cortical actin network of the egg does not form, yet contractile rings appear. Coinjection of RNA encoding the betaC or chicken beta1 subunit, but lacking the morpholino target sequence, rescues the cortical actin network and normal embryos result. Coinjection of RNA encoding the betaC subunit lacking the cytoplasmic domain fails to rescue. These studies demonstrate that the cortical actin cytoskeleton is anchored by betaC integrins and contractile ring actin is not. We suggest that one important function of egg integrins is to organize the actin cortex.
Burke
Robert D
RD
Departments of Biology and Biochemistry/Microbiology, University of Victoria, Victoria, BC, Canada. rburke@uvic.ca
Murray
Greg
G
Rise
Matthew
M
Wang
Diana
D
D000199:Actins / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D003599:Cytoskeleton / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000502:physiology; D039641:Integrin beta Chains / Q000502:physiology*; D008845:Microinjections; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010063:Ovum / Q000166:cytology* / Q000502:physiology; D012617:Sea Urchins / Q000196:embryology* / Q000502:physiology
D000199:Actins; D016375:Antisense Elements (Genetics); D039641:Integrin beta Chains