title: Capillary electrophoresis method for simultaneous determination of penicillin G, procaine and dihydrostreptomycin in veterinary drugs.
abstract: Capillary electrophoresis method for identification and simultaneous determination of procaine, dihydrostreptomycin and penicillin G, present in multiantibiotic veterinary preparations, was elaborated. The influence of pH (5.0-9.75) and concentration of disodium tetraborate decahydrate in running buffers (0.02-0.1 M) as well as temperatures (25-40 degrees C) on separation efficacy were analyzed. For quantitative analysis, 0.08 M borate buffer (pH 8.0) at 35 degrees C and 15 kV were chosen. Method was validated, selectivity, precision, linearity, LOD, LOQ, accuracy and specificity of capillary zone electrophoresis (CZE) were evaluated.
authors.affiliation: Antibiotics and Microbiology Department, National Institute of Public Health, 30-34 Chelmska Street, 00-725 Warsaw, Poland. tyski@il.waw.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michalska
authors.forename: Genowefa
authors.initials: G
authors.lastname: Pajchel
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
mesh_terms: D002021:Buffers; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis*; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D010402:Penicillin G Procaine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D019155:Veterinary Drugs; D010402:Penicillin G Procaine; D004096:Dihydrostreptomycin Sulfate