title: Adenosine 5'-(gamma-thio) triphosphate (ATPgammaS) stimulates both P2Y receptors linked to inositol phosphates production and cAMP accumulation in bovine adrenocortical fasciculata cells.
abstract: The study was aimed to investigate the existence of at least two kinds of P2Y receptors linked to steroidogenesis in bovine adrenocortical fasciculata cells (BAFCs). Extracellular nucleotides facilitated steroidogenesis in BAFCs. The potency order was UTP > adenosine 5'-(gamma-thio) triphosphate (ATPgammaS) > ATP > 2-methylthio ATP (2MeSATP) > adenosine 5'-(beta-thio) diphosphate (ADPbetaS) > alpha,beta-methylene ATP (alpha,beta-me-ATP), beta,gamma-methylene ATP (beta,gamma -me-ATP). ATPgammaS (10-100 microM) remarkably stimulated both total inositol phosphates (IPs) production and cyclic AMP (cAMP) accumulation. Competitive displacement experiments by using [35S]ATPgammaS as a radioactive ligand in BAFCs showed that the potency under these unlabelled ligands was ATPgammaS > ATP > ADPbetaS > 2MeSATP > UTP > alpha,beta-me-ATP, beta,gamma-me-ATP. These suggest that two different binding sites of [35S]ATPgammaS, namely P2Y receptors, exist in BAFCs, and that these receptors are linked to steroidogenesis via distinct second messenger systems in the cells.
authors.affiliation: Department of Pharmacology I, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato, Tokyo 105-8461, Japan. hnishi@jikei.ac.jp
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Nishi
authors.forename: Seiji
authors.initials: S
authors.lastname: Hori
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Niitsu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawamura
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D007295:Inositol Phosphates / Q000096:biosynthesis*; D018048:Receptors, Purinergic P2 / Q000187:drug effects* / Q000378:metabolism; D015383:Zona Fasciculata / Q000166:cytology / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; D018048:Receptors, Purinergic P2; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D000242:Cyclic AMP