Microtubules-associated intracellular localization of the NH2-terminal cellular prion protein fragment.
By utilizing double-labeled fluorescent cellular prion protein (PrPC), we revealed that the NH2-terminal and COOH-terminal PrPC fragments exhibit distinct distribution patterns in mouse neuroblastoma neuro2a (N2a) cells and HpL3-4, a hippocampal cell line established from prnp gene-ablated mice [Nature 400 (1999) 225]. Of note, the NH2-terminal PrPC fragment, which predominantly localized in the intracellular compartments, congregated in the cytosol after the treatment with a microtubule depolymerizer (nocodazole). Truncated PrPC with the amino acid residues 1-121, 1-111, and 1-91 in mouse (Mo) PrP exhibited a proper distribution profile, whereas those with amino acid residues 1-52 and 1-33 did not. These data indicate the microtubules-associated intracellular localization of the NH2-terminal PrPC fragment containing at least the 1-91 amino acid residues.
Hachiya
Naomi S
NS
Department of Cortical Function Disorders, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan.
Watanabe
Kota
K
Sakasegawa
Yuji
Y
Kaneko
Kiyotoshi
K
D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D018621:PrPC Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D014404:Tubulin / Q000378:metabolism
D008164:Luminescent Proteins; D018621:PrPC Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins; D004247:DNA; D015739:Nocodazole