Production and characterization of monoclonal antibodies with specificity for human HLA-G isoforms.
Production of monoclonal antibodies to HLA-G, a nonpolymorphic antigen of non-classical HLA class I, is of basic and clinical importance. In the present study, monoclonal antibodies were prepared which recognize different membrane bound and soluble isoforms of HLA-G, following immunization of BALB/c mice with a synthetic peptide. Use of this peptide (23 residues), which is present in the alpha1 domain of HLA-G, was previously demonstrated to provide antibodies useful for recognition of HLA-G isoforms in human placenta. Antibody-producing hybridomas were screened by an indirect one-step ELISA method. A clone designated 5E6H7, secreted antibodies useful in immunostaining studies involving both surface HLA-G of placental tissues and soluble forms of this antigen in human sera. In addition, unreactivity of this antibody with human lymphocytes and sections of normal human skin was observed by immunofluorescence microscopy, thus demonstrating its specificity for HLA-G.
Yari
Fatemeh
F
Department of Immunology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
Hosseini
Ahmad Zavaran
AZ
Nemat-Gorgani
Mohsen
M
Sareh
Shahla
S
Khorramizadeh
Mohammad Reza
MR
Mansouri
Parvin
P
Nejad
Anoshirvan Kazem
AK
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000096:biosynthesis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000918:Antibody Specificity; D046508:Culture Techniques; D005260:Female; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000276:immunology; D020033:Protein Isoforms
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D020033:Protein Isoforms