title: Structural investigation of proapoptotic peptide by CD and NMR spectroscopy.
abstract: We have performed a systematic investigation of the structural features of the peptides Int (a sequence able to cross cell membranes) and Int-H1(S6A,F8A) (which shows interesting antitumoral properties). After screening in aqueous solution at different ionic strength and pH values, we analyzed the structures of the peptides in different water/trifluoroethanol mixtures by Circular Dichroism and Nuclear Magnetic Resonance techniques.
authors.affiliation: Department of Biophysical Sciences and Technologies M.&O., University of Genoa, c.so Europa 30, Genoa, Italy.
authors.forename: Emy
authors.initials: E
authors.lastname: Pulsinelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Vasile
authors.forename: Laura
authors.initials: L
authors.lastname: Vergani
authors.forename: Silvio
authors.initials: S
authors.lastname: Parodi
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry; D017209:Apoptosis; D002942:Circular Dichroism; D015195:Drug Design; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors; D012996:Solutions; D014270:Trifluoroethanol; D014867:Water; D051153:Wnt Proteins
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012996:Solutions; D051153:Wnt Proteins; D014867:Water; D014270:Trifluoroethanol