title: Mitochondrial DNA heteroplasmy in calves cloned by using adult somatic cell.
abstract: Adult somatic cell cloned calves were produced by somatic cell nuclear transfer prepared by fusion of cultured ear fibroblast from a Holstein cow into enucleated oocytes of Luxi Yellow cow. In order to determinate the source of mitochondrial DNA of cloned calves, we designed the breed-specific PCR primers by aligning the known D-loop sequences of Bos taurus and analyzed the displacement loop sequences of five live cloned calves by breed-specific primers PCR. The results demonstrated that mtDNA originated from Holstein breed and that from Luxi breed co-exist in all five live calves.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Han
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Wan
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Kou
authors.forename: Gang
authors.initials: G
authors.lastname: Rao
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: Fang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D019976:Cloning, Organism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D053652:Nuclear Transfer Techniques; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial