title: Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals.
abstract: In mammals, the Y-located SRY gene is known to induce testis formation from the indifferent gonad. A related gene, SOX9, also plays a critical role in testis differentiation in mammals, in birds and reptiles. It is now assumed that SRY acts upstream of SOX9 in the sex determination cascade, but the regulatory link which should exist between these two genes remains unknown. Studies on XX sex reversal in polled goats (PIS mutation: Polled Intersex Syndrome) have led to the discovery of a female-specific locus crucial for ovarian differentiation. This genomic region is composed of at least two genes, FOXL2 and PISRT1, which share a common transcriptional regulatory region, PIS. In this review, we present the expression pattern of these PIS-regulated genes in mice. The FOXL2 expression profile of mice is similar to that described in goats in accordance with a conserved role of this ovarian differentiating gene in mammals. On the contrary, the PISRT1 expression profile is different between mice and goats, suggesting different mechanisms of the primary switch in the testis determination process within mammals. A model based on two different modes of SOX9 regulation in mice and other mammals is proposed in order to integrate our results into the current scheme of gonad differentiation.
authors.affiliation: Biologie du Développement et Reproduction, INRA Bât J. Poly, Jouy-en-Josas, France.
authors.forename: M
authors.initials: M
authors.lastname: Pannetier
authors.forename: N
authors.initials: N
authors.lastname: Servel
authors.forename: J
authors.initials: J
authors.lastname: Cocquet
authors.forename: N
authors.initials: N
authors.lastname: Besnard
authors.forename: C
authors.initials: C
authors.lastname: Cotinot
authors.forename: E
authors.initials: E
authors.lastname: Pailhoux
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012734:Disorders of Sex Development; D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D005786:Gene Expression Regulation; D006041:Goats; D006066:Gonads / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D008322:Mammals / Q000235:genetics*; D051379:Mice; D009154:Mutation; D009687:Nuclear Proteins; D012045:Regulatory Sequences, Nucleic Acid; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; C498893:Foxl2 protein, mouse; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors