title: The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas.
abstract: By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.
authors.affiliation: Lab of Cancer Genetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
authors.forename: Jindong
authors.initials: J
authors.lastname: Chen
authors.forename: Weng-Onn
authors.initials: WO
authors.lastname: Lui
authors.forename: Michele D
authors.initials: MD
authors.lastname: Vos
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Clark
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Schoumans
authors.forename: Sok Kean
authors.initials: SK
authors.lastname: Khoo
authors.forename: David
authors.initials: D
authors.lastname: Petillo
authors.forename: Todd
authors.initials: T
authors.lastname: Lavery
authors.forename: Jun
authors.initials: J
authors.lastname: Sugimura
authors.forename: Dewi
authors.initials: D
authors.lastname: Astuti
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Susumu
authors.initials: S
authors.lastname: Kagawa
authors.forename: Eamonn R
authors.initials: ER
authors.lastname: Maher
authors.forename: Catharina
authors.initials: C
authors.lastname: Larsson
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Alberts
authors.forename: Hiro-omi
authors.initials: HO
authors.lastname: Kanayama
authors.forename: Bin Tean
authors.initials: BT
authors.lastname: Teh
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019175:DNA Methylation; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; C454042:RASSF5 protein, human; C095362:limbic system-associated membrane protein; D020559:Monomeric GTP-Binding Proteins