Determination of trivalent methylated arsenicals in rat urine by liquid chromatography-inductively coupled plasma mass spectrometry after solvent extraction.
A method for the determination of trivalent arsenicals in urine was examined. Trivalent arsenicals, extracted as complexes with diethylammonium diethyldithiocarbamate (DDDC) into carbon tetrachloride, were determined by liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS). The trivalent methylated arsenicals monomethylarsonous acid (MMA(III)), dimethylarsinous acid (DMA(III)), and trimethylarsine (TMA) were detected in urine of rats that had received dimethylarsinic acid (DMA(V)) or monomethylarsonic acid (MMA(V)) at concentration of 200 microg ml(-1) in drinking water for 24 weeks. This method is the first to permit quantification of trivalent methylated arsenicals in urine without significant changes in concentration during storage or pretreatment.
Okina
Masako
M
Department of Preventive Medicine and Environmental Health, Osaka City University Medical School, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan. m-okina@med.osaka-cu.ac.jp
Yoshida
Kaoru
K
Kuroda
Koichi
K
Wanibuchi
Hideki
H
Fukushima
Shoji
S
Endo
Ginji
G
D000818:Animals; D001152:Arsenicals / Q000008:administration & dosage / Q000652:urine*; D002101:Cacodylic Acid / Q000008:administration & dosage; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents
D001152:Arsenicals; D012997:Solvents; D002101:Cacodylic Acid; C020300:monomethylarsonic acid