Sleep deprivation effects on growth factor expression in neonatal rats: a potential role for BDNF in the mediation of delta power.
The sleeping brain differs from the waking brain in its electrophysiological and molecular properties, including the expression of growth factors and immediate early genes (IEG). Sleep architecture and homeostatic regulation of sleep in neonates is distinct from that of adults. Hence, the present study addressed the question whether the unique homeostatic response to sleep deprivation in neonates is reflected in mRNA expression of the IEG cFos, brain-derived nerve growth factor (BDNF), and basic fibroblast growth factor (FGF2) in the cortex. As sleep deprivation is stressful to developing rats, we also investigated whether the increased levels of corticosterone would affect the expression of growth factors in the hippocampus, known to be sensitive to glucocorticoid levels. At postnatal days 16, 20, and 24, rats were subjected to sleep deprivation, maternal separation without sleep deprivation, sleep deprivation with 2 h recovery sleep, or no intervention. mRNA expression was quantified in the cortex and hippocampus. cFos was increased after sleep deprivation and was similar to control level after 2 h recovery sleep irrespective of age or brain region. BDNF was increased by sleep deprivation in the cortex at P20 and P24 and only at P24 in the hippocampus. FGF2 increased during recovery sleep at all ages in both brain regions. We conclude that cortical BDNF expression reflects the onset of adult sleep-homeostatic response, whereas the profile of expression of both growth factors suggests a trophic effect of mild sleep deprivation.
Hairston
Ilana S
IS
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA. Ilana.Hairston@stanford.edu
Peyron
Christelle
C
Denning
Daniel P
DP
Ruby
Norman F
NF
Flores
Judith
J
Sapolsky
Robert M
RM
Heller
H Craig
HC
O'Hara
Bruce F
BF
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism / Q000502:physiology; D015152:Blotting, Northern / Q000379:methods; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D016009:Chi-Square Distribution; D003345:Corticosterone / Q000097:blood; D003700:Delta Rhythm; D004576:Electromyography / Q000379:methods; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D016762:Genes, fos / Q000502:physiology; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism; D008027:Light; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D020318:Rats, Long-Evans; D012892:Sleep Deprivation / Q000378:metabolism* / Q000503:physiopathology; D014851:Wakefulness / Q000502:physiology; D020813:Receptor, trkB
D019208:Brain-Derived Neurotrophic Factor; D003345:Corticosterone; D016222:Fibroblast Growth Factor 2; D006133:Growth Substances; D008562:Membrane Glycoproteins; D011505:Protein-Tyrosine Kinases; D012333:RNA, Messenger; C064371:Ntrk2 protein, mouse; D020813:Receptor, trkB