Cell-permeable small molecule probes for site-specific labeling of proteins.
We have successfully synthesized a number of small molecule probes designed for site-specific labeling of N-terminal cysteine-containing proteins expressed in live cells. Their utility for site-specific, covalent modifications of proteins was successfully demonstrated with purified proteins in vitro, and with live bacterial cells in vivo.
Yeo
Dawn S Y
DS
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
Srinivasan
Rajavel
R
Uttamchandani
Mahesh
M
Chen
Grace Y J
GY
Zhu
Qing
Q
Yao
Shao Q
SQ
D002463:Cell Membrane Permeability; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005786:Gene Expression Regulation; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins; D003545:Cysteine