[Cytotoxic effect and influence of povidone-iodine on wounds in guinea pig].
Cytotoxicity and effect on guinea pig wounds were compared with three kinds of 10% povidone-iodine solution (isodine, neojodine, J-iodine) and povidone-iodine. It gave the following results: 1. In the Chang conjunctiva cell where remarkable toxicity was observed in the used cell line, the toxicity emphasized was 10 times using povidone-iodine < J-iodine < neojodine < isodine. In addition, a toxic difference was recognized between cells, in the order of Chang conjunctiva > SIRC > FRSK > human fibroblast for isodine and neojodine. 2. As for the influence on cells achromatized in sodium thiosulfate, alteration was not recognized in isodine, J-iodine using half-maximum cytotoxicity concentrations (CC50), and, in neojodine and povidone-iodine, elimination of drug toxicity was recognized in Chang conjunctiva/FRSK cells. 3. Strong toxicity was seen in isodine and neojodine in the toxicity test using colony formation method, however on washing with PBS (-), an easing of the toxicity effect could be seen to 0.01%. 4. Polyoxythylene nonylphenyl ether, NP-10 was the most toxic in the used surfactants in this study, and the toxicity by 100 times when compared with sodium polyoxyethylene lauryl ether sulfate, SBL-2N. Prominent sensitivity was observed in Chang conjunctiva cells with regard to the cell line and subsequently showed toxicity in order of SIRC > FRSK > human fibroblast. Remarkably, the difference between solutions of isodine and J-iodine was made more clear from the 5th culture. 5. In this study, significantly guinea pig wounds, povidone-iodine blocked all examples significantly recognized as dallying by distance between epidermal cells. In addition, isodine was meaningful area. Although their ware not offer the significant differences, the solutions obstructed the sliding of epidermal cells in comparison with povidone-iodine, and a delaying tendency of inflammation was also recognized. The toxic difference between povidone iodine solutions was regarded as the influence of surfactants added to the povidone-iodine. Based on this foundation study, more detailed clinical testing is necessary in determine where sterilization to wounds/mucosa/eyes with solutions containing additive is a suitable panpractice or not.
Iwasawa
Atsuo
A
Showa University Fujigaoka Hospital, Department of Clinical Pathology.
Nakamura
Yoshiko
Y
D000818:Animals; D003228:Conjunctiva / Q000166:cytology; D003713:Dendritic Cells; D006168:Guinea Pigs; D011206:Povidone-Iodine / Q000633:toxicity*; D013501:Surface-Active Agents / Q000494:pharmacology; D014947:Wounds and Injuries / Q000473:pathology*
D013501:Surface-Active Agents; D011206:Povidone-Iodine