Hepatocellular apoptosis after hepatectomy in obstructive jaundice in rats.
OBJECTIVE
To investigate the hepatocellular apoptosis after hepatectomy in obstructive jaundice and biliary decompression rats.


METHODS
After bile duct ligation for 7 days, rats were randomly divided into OB group in which the rats underwent 70% hepatectomy, OB-CD group in which the rats underwent hepatectomy accompanied by choledochoduodenostomy, CD-Hx group in which the rats underwent choledochoduodenostomy and then received 70% hepatectomy on the fifth day after biliary decompression. The control group (Hx group) only underwent hepatectomy.


RESULTS
The level of total serum bilirubin and serum enzymes was significantly lower in CD-Hx group than in OB-CD and OB groups on day 1, 3 and 5 after hepatectomy. The apoptotic index was significantly lower in CD-Hx group than in OB-CD and OB groups on day 3 and 5. The oligonucleosomal DNA fragments and Caspase-3 activity were also lower in CD-Hx group than in OB-CD and OB groups 3 days after hepatectomy, without differences between CD-Hx and Hx groups.


CONCLUSIONS
Hepatocellular apoptosis plays vital roles in jaundice rats, and biliary decompression is more effective in treatment of patients with severe jaundice before operation.
Wang
De-Sheng
DS
Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, Shannxi Province, China. wangdesh@163.com
Dou
Ke-Feng
KF
Li
Kai-Zong
KZ
Gao
Zhi-Qing
ZQ
Song
Zhen-Shun
ZS
Liu
Zheng-Cai
ZC
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001663:Bilirubin / Q000097:blood; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002768:Choledochostomy; D004195:Disease Models, Animal; D006498:Hepatectomy; D041781:Jaundice, Obstructive / Q000097:blood / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D001663:Bilirubin