title: A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product.
authors.affiliation: Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary.
authors.forename: Zsolt B
authors.initials: ZB
authors.lastname: Nagy
authors.forename: Ferenc
authors.initials: F
authors.lastname: Felföldi
authors.forename: László
authors.initials: L
authors.lastname: Tamás
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
mesh_terms: D004252:DNA Mutational Analysis; D004262:DNA Restriction Enzymes; D008722:Methods; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D004262:DNA Restriction Enzymes