title: Induction of PYPAF1 during in vitro maturation of mouse mast cells.
abstract: Coculture of mouse bone marrow-derived immature mast cells (BMMC) with Swiss 3T3 fibroblasts in the presence of stem cell factor (SCF) promotes morphological and functional maturation toward a connective tissue mast cell (CTMC)-like phenotype, which is accompanied by increased expression of several unique genes. Here we report the molecular identification of one of them, mast cell maturation-associated inducible gene (MMIG)-1. The MMIG-1 cDNA encodes a 117-kDa cytosolic protein that comprises an N-terminal PYRIN domain, a central nucleotide-binding domain, and nine C-terminal leucine-rich repeats. MMIG-1 shows >85% sequence similarity to human cryopyrin/PYPAF1, a causal gene for familial cold urticaria and Muckle-Wells syndrome. MMIG-1 was distributed in the cytosol of CTMC-like differentiated BMMC. MMIG-1 underwent alternative splicing in the leucine-rich repeats and each variant was induced differently in BMMC during coculture. Moreover, its expression was increased in the ears of mice with experimental atopic dermatitis. Thus, MMIG-1, a likely mouse PYPAF1 ortholog, may play a role in mast cell-directed inflammatory diseases.
authors.affiliation: Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555.
authors.forename: Rei
authors.initials: R
authors.lastname: Kikuchi-Yanoshita
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Taketomi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Koga
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Sugiki
authors.forename: Yohei
authors.initials: Y
authors.lastname: Atsumi
authors.forename: Takanori
authors.initials: T
authors.lastname: Saito
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Ishii
authors.forename: Masato
authors.initials: M
authors.lastname: Hisada
authors.forename: Tamiko
authors.initials: T
authors.lastname: Suzuki-Nishimura
authors.forename: Masaatsu K
authors.initials: MK
authors.lastname: Uchida
authors.forename: Tae-Chul
authors.initials: TC
authors.lastname: Moon
authors.forename: Hyeun-Wook
authors.initials: HW
authors.lastname: Chang
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Sawada
authors.forename: Naoki
authors.initials: N
authors.lastname: Inagaki
authors.forename: Hiroichi
authors.initials: H
authors.lastname: Nagai
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kudo
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D003001:Cloning, Molecular; D018920:Coculture Techniques; D018076:DNA, Complementary / Q000235:genetics; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000235:genetics; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D004139:Dinitrofluorobenzene; D012333:RNA, Messenger; C492716:Nlrp3 protein, mouse; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein