Characterization in maize of ZmTIP2-3, a root-specific tonoplast intrinsic protein exhibiting aquaporin activity.
The characterization of the tonoplast intrinsic protein ZmTIP2-3 cDNA isolated from maize roots is reported. ZmTIP2-3 belongs to the TIP2 group according to the present nomenclature. The aquaporin function of ZmTIP2-3 protein was demonstrated using expression in X. laevis oocytes. Northern blot analyses revealed that ZmTIP2-3 was specifically expressed in roots. Salt and water stresses induced the accumulation of ZmTIP2-3 transcripts. By contrast, no effect of ABA was observed. An oscillation of ZmTIP2-3 transcript amount during the day-night cycle was observed with some typical features of genes regulated by a circadian mechanism.
Lopez
Félicie
F
Institut de Biotechnologie des Plantes, Bât. 630, CNRS/UMR 8618, Université Paris-Sud, F-91405 Orsay Cedex, France.
Bousser
Agnès
A
Sissoëff
Igor
I
Hoarau
Jackson
J
Mahé
Aline
A
D020346:Aquaporins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D020346:Aquaporins; D017931:DNA Primers; D010940:Plant Proteins; D018749:RNA, Plant; D012337:RNA, Ribosomal, 18S