Diffusion of single cardiac ryanodine receptors in lipid bilayers is decreased by annexin 12.
Diffusion of cardiac ryanodine receptors (RyR2) in lipid bilayers was characterized. RyR2 location was monitored by imaging fluo-3 fluorescence due to Ca2+ flux through RyR2 channels or fluorescence from RyR2 conjugated with Alexa 488 or containing green fluorescent protein. Single channel currents were recorded to ensure that functional channels were studied. RyR2 exhibited an apparent diffusion coefficient (DRyR) of 1.2 x 10(-8) cm2 s(-1) and a mean path length of 5.0 microm. Optimal use of optical methods for analysis of RyR2 channel function requires that RyR2 diffusion be limited. Therefore, we tested the effect of annexin 12, which interacts with anionic phospholipids in a Ca2+-dependent manner. Addition of annexin 12 (0.25-4.0 microM) to the trans side of bilayers containing an 80:20 ratio of phosphatidylethanolamine/phosphatidylserine decreased RyR2 diffusion in a concentration-dependent manner. Annexin 12 (2 microM) decreased the apparent DRyR 683-fold from 1.2-10(-8) to 1.8 x 10(-11) cm2 s(-1) and the mean path length 10-fold from 5.0 to 0.5 micro m without obvious changes in the conductance of the native bilayer or in activation of RyR2 channels by Ca2+ or suramin. Thus, annexin 12 may provide a useful tool for optimizing optical analysis of RyR2 channels in lipid bilayers.
Peng
S
S
Department of Pharmacology, University of Nevada, Reno, Nevada 89557, USA.
Publicover
N G
NG
Airey
J A
JA
Hall
J E
JE
Haigler
H T
HT
Jiang
D
D
Chen
S R Wayne
SR
Sutko
J L
JL
D000818:Animals; D017302:Annexins / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D004058:Diffusion; D004553:Electric Conductivity; D008051:Lipid Bilayers / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry*
C488832:ANXB12 protein, Hydra attenuata; D017302:Annexins; D008051:Lipid Bilayers; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium