[Effects of beta-asaron on gene expression in mouse brain].
OBJECTIVE
To observe effects of beta-asaron on gene expression in BALB/c mouse brain by gene chip.


METHODS
The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft.


RESULTS
Among 4000 genes, there are 15 genes with difference expression (the ratio > 1.9 or < 0.59) in two groups. In beta-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism.


CONCLUSIONS
beta-asaron has effects on multi-target genes in mouse brain.
Fang
Yongqi
Y
First Affiliated Hospital, Guangzhou University, TCM, Guangzhou 510405.
Li
Ling
L
Wu
Qiduan
Q
D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D029064:Araceae / Q000737:chemistry*; D001921:Brain / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010946:Plants, Medicinal / Q000737:chemistry
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D004365:Drugs, Chinese Herbal; C012195:asarone