C-fos expression in rat brain nuclei following incisor tooth movement.
In the rat experimental model, molar tooth movement induced by Waldo's method is known to cause a temporally and spatially defined pattern of brain neuronal activation. Since orthodontic correction usually involves the entire dental arch, we used a spring-activated appliance to extend the investigation to incisors, and we included brain regions related to antinociception. Adjustment of the non-activated appliance on incisors resulted in c-fos expression in the dorsal raphe, peri-aqueductal gray matter, and the locus coeruleus, in addition to trigeminal sensory subnuclei and the parabrachial nucleus, where neuronal activation has already been detected in previous studies on molar tooth movement. Appliance activation with a 70-g force resulted in a further increase in Fos-immunoreactive neurons in the trigeminal sensory subnucleus caudalis and in the dorsal raphe. This result suggests that there is a recruitment of neurons related to nociception and to antinociception when tooth movement is increased.
Magdalena
C M
CM
Department of Morphology, Stomatology and Physiology, Dental School of Ribeirão Preto, University of São Paulo, Avenida do Café s/n, CEP 14040-904, Ribeirão Preto, SP, Brazil.
Navarro
V P
VP
Park
D M
DM
Stuani
M B S
MB
Rocha
M J A
MJ
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D016762:Genes, fos / Q000187:drug effects / Q000235:genetics*; D007180:Incisor / Q000473:pathology*; D007649:Ketamine / Q000494:pharmacology; D008125:Locus Coeruleus / Q000378:metabolism / Q000473:pathology; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D009619:Nociceptors / Q000378:metabolism / Q000648:ultrastructure; D009967:Orthodontic Appliances; D010146:Pain / Q000235:genetics; D010487:Periaqueductal Gray / Q000378:metabolism / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D011999:Recruitment, Neurophysiological / Q000235:genetics; D012154:Reticular Formation / Q000378:metabolism / Q000473:pathology; D014087:Tooth Movement Techniques / Q000295:instrumentation; D014278:Trigeminal Nuclei / Q000378:metabolism / Q000473:pathology; D014991:Xylazine / Q000494:pharmacology
D000700:Analgesics; D016760:Proto-Oncogene Proteins c-fos; D014991:Xylazine; D007649:Ketamine