title: Drosophila melanogaster larval hemolymph protein mapping.
abstract: With the completion of the genome sequence of Drosophila melanogaster the importance of constructing a proteome map is to be considered. Therefore, with the application of recent advances in proteomic analysis approaches, a protein map of D. melanogaster larvae hemolymph proteins was obtained using 2-DE in the range of pH 3-10. After Coomassie colloidal detection of 289 spots, a total of 105 were excised from the gel and digested with trypsin. Identification was done based on a combination of MALDI-TOF/TOF MS and MS/MS spectra. The 99 proteins identified using this approach include a large number of metabolic enzymes, translational apparatus components, and structural proteins. Among these we emphasize the identification of proteins with molecular chaperone properties (heat shock proteins and PPIases) and protein spots involved in defense responses such as antioxidant and immunological defense mechanisms (thioredoxin, prophenoloxidase, and serine proteases), as well as in signal transduction pathways.
authors.affiliation: Department of Chemistry, University of Aveiro, Aveiro, Portugal.
authors.forename: Sofia de Morais
authors.initials: Sde M
authors.lastname: Guedes
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.forename: Kenneth
authors.initials: K
authors.lastname: Tomer
authors.forename: M Rosário M
authors.initials: MR
authors.lastname: Domingues
authors.forename: A J Ferrer
authors.initials: AJ
authors.lastname: Correia
authors.forename: Francisco
authors.initials: F
authors.lastname: Amado
authors.forename: Pedro
authors.initials: P
authors.lastname: Domingues
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030562:Databases, Protein; D029721:Drosophila Proteins / Q000032:analysis / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006458:Hemolymph / Q000737:chemistry* / Q000378:metabolism*; D007814:Larva / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029721:Drosophila Proteins; D020543:Proteome