title: Proteome analysis of glial cells treated by radiation or hydroperoxide.
abstract: Reactive oxygen species (ROS) give rise to various types of oxidative damage and some responses such as activation of transcription factors and/or repair factors in cells. Proteome analysis using 2-D PAGE and peptide mass fingerprinting is suitable for the study on the post-translational modifications of proteins. When glial cells were exposed to hydroperoxide, the relative abundance of 9 spots changed on 2-D gels, as compared with control gels. MALDI-TOF MS analysis after in-gel digestion revealed that these spots corresponded to at least 3 pairs of proteins. These pairs of protein spots had different isoelectric points each other and were identified as peroxiredoxin II, peroxiredoxin III and calpactin I light chain. It was demonstrated that peroxiredoxins II and III were oxidized to their acidic forms (acidic spots) by hydroperoxide, and thereafter the native forms (basic spots) were recovered as the acidic forms disappeared.
authors.affiliation: Tokyo Metropolitan Institute of Gerontology.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Miura
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kano
authors.forename: Tosifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Shiro
authors.initials: S
authors.lastname: Urano
authors.forename: Shozo
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D043383:Adenosylhomocysteinase / Q000378:metabolism; D000818:Animals; D017306:Annexin A2 / Q000378:metabolism; D001253:Astrocytes / Q000187:drug effects*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology*; D054464:Peroxiredoxins / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017306:Annexin A2; D020543:Proteome; D003513:Cycloheximide; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D010741:Phospholipases A; D043383:Adenosylhomocysteinase