Effects of recombinant human growth hormone and nandrolone phenylpropionate on the healing of ischemic colon anastomosis in rats.
OBJECTIVE
Recombinant human growth hormone and nandrolone phenylpropionate are two different anabolic agents. This study was designed to investigate the effects of these anabolic agents on the healing of ischemic colon anastomosis in rats.


METHODS
Seventy adult male Wistar rats were divided into five groups (n = 14). Group I was the sham laparotomy group. In the other groups, surgical procedures consisting of transsection and anastomosis were made at a distance 3 cm from the peritoneal reflection. Group II was the nonischemic control group. Ischemic colon model was produced in the remaining groups. Group III was the untreated control group. Groups IV and V received recombinant human growth hormone and nandrolone phenylpropionate, respectively. Bursting pressure and hydroxyproline levels were measured on the third and seventh postoperative days to evaluate anastomotic healing.


RESULTS
Recombinant human growth hormone increased both collagen deposition and bursting pressure significantly at postoperative Days 3 and 7 compared with the sham and untreated control groups (P < 0.005). When compared with the untreated control, nandrolone phenylpropionate significantly increased collagen deposition at postoperative Days 3 and 7 (P < 0.005) and bursting pressure only at postoperative Day 3 (P < 0.005).


CONCLUSIONS
Recombinant human growth hormone has more favorable therapeutic effects on the healing of ischemic colonic anastomoses than nandrolone phenylpropionate. Recombinant human growth hormone also improves healing of nonischemic colonic anastomosis.
Yarimkaya
Ali
A
Department of General Surgery, SSK Hospital, Karamursel, Turkey.
Apaydin
Berat
B
Unal
Ethem
E
Karabicak
Ilhan
I
Aydogan
Fatih
F
Uslu
Ezel
E
Erginoz
Ethem
E
Artis
Tarik
T
Eyuboglu
Erhun
E
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000098:blood supply / Q000601:surgery*; D004195:Disease Models, Animal; D019382:Human Growth Hormone / Q000494:pharmacology*; D007511:Ischemia / Q000209:etiology / Q000517:prevention & control; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
D019382:Human Growth Hormone; C015413:nandrolone acetate; D009277:Nandrolone