title: Imaging of (13)C-labeled glucose and sorbitol in bovine lens by (1)H-detected (13)C nuclear magnetic resonance spectroscopy.
abstract: Bovine lenses were incubated in a solution containing [1-(13)C]glucose (50 mM) for 1, 2 and 4 days. Spectroscopic images of [1-(13)C]glucose and [1-(13)C]sorbitol were constructed using (1)H-detected gradient-enhanced heteronuclear multiple-quantum coherence (GE-HMQC) in a 2.0-tesla magnetic field. Accumulations of [1-(13)C]glucose and [1-(13)C]sorbitol were mainly observed at the periphery of the lens. Their distributions corresponded to the cortex. (1)H-detected (13)C nuclear magnetic resonance (NMR) spectroscopic imaging by GE-HMQC successfully demonstrated the distribution of [1-(13)C]glucose and [1-(13)C]sorbitol at the periphery of bovine lenses.
authors.affiliation: Department of Ophthalmology, Shiga University of Medical Science, Shiga, Japan. tsawada@belle.shiga-med.ac.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sawada
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakamura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kani
authors.forename: Ryohei
authors.initials: R
authors.lastname: Okamoto
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Morikawa
authors.forename: Toshiro
authors.initials: T
authors.lastname: Inubushi
mesh_terms: D000818:Animals; D002417:Cattle; D005947:Glucose / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D023421:Models, Animal; D013012:Sorbitol / Q000378:metabolism*
chemical_list: D013012:Sorbitol; D005947:Glucose