title: Cl- channels in basolateral TAL membranes. XIX. Cytosolic Cl- regulates mmCIC-Ka and mcCIC-Ka channels.
abstract: We evaluated the effects of culturing mouse MTAL cells under conditions that suppressed steady-state cytosolic Cl- on chloride channels fused into bilayers from basolateral vesicles of cultured MTAL cells. We used two agents to suppress Cl- entry: 10(-6) M PGE2 and 10(-4) M bumetanide. Basolateral Cl- channels from control cultured MTAL cells exhibited the signature characteristics of mmCIC-Ka channels: increased open-time probability (Po) either by raising cytosolic-face [Cl-] or, at 2 mM cytosolic Cl-, by adding (ATP + PKA), and first-order conductance kinetics. Either 10(-6) M PGE2 or 10(-4) M bumetanide in culture media reduced steady-state MTAL cytosolic Cl-. Chloride channels from these cells exhibited characteristics unique to CTAL mcCIC-Ka channels, namely: no augmentation of Po either by raising cytosolic Cl- or with cytosolic (ATP + PKA), and multi-ion occupancy. Semi-quantitative RT-PCR and real-time quantitative PCR showed that culturing MTAL cells with 10(-6) M PGE2 or 10(-4) M bumetanide reduced mRNA levels encoding mmCIC-Ka but not mRNA levels encoding mcCIC-Ka. However, when MTAL cells were cultured under control conditions, and then pre-incubated for 60 minutes with 10(-4) M bumetanide, cytosolic Cl- fell acutely but Cl- channels exhibited characteristics of mmCIC-Ka channels. Thus PGE2 and bumetanide, both of which lower steady-state MTAL cytosolic Cl- concentrations, inhibit either the transcriptional and/or the translational processes for mmCIC-Ka synthesis.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, University of Arkansas College of Medicine, and The Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Winters
authors.forename: M V
authors.initials: MV
authors.lastname: Mikhailova
authors.forename: T E
authors.initials: TE
authors.lastname: Andreoli
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D002034:Bumetanide / Q000494:pharmacology; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000378:metabolism; D008564:Membrane Potentials; D051379:Mice; D010120:Oxytocics / Q000494:pharmacology; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D004232:Diuretics; D008051:Lipid Bilayers; D010120:Oxytocics; D015221:Potassium Channels; D012333:RNA, Messenger; D002034:Bumetanide; D000255:Adenosine Triphosphate; D017868:Cyclic AMP-Dependent Protein Kinases; D015232:Dinoprostone