[Study on making pneumoconiosis model and method of lung lavage in rabbit].
OBJECTIVE
To provide an intact rabbit pneumoconiosis model and a method of lung lavage for studying the pathogenesis and treatment of pneumoconiosis.


METHODS
The dust particles(< or = 5 microns diameter) were poured into the trachea of rabbit by trachea spile. The lung lavage of rabbit was carried out by the improved trachea catheter method.


RESULTS
The rabbit lungs exposed to coal dust showed many black dust spots and there were proliferation of fibroblasts. The rabbit lungs exposed to silica dust showed nodules and several fusing nodules. The X-ray film showed that there were small irregular shadows, or fusing together into flake-like or round shadows in both lungs. The recovery rate of the lung lavage was 89%-94%.


CONCLUSIONS
The model made by trachea spile and the method of lung lavage by the improved trachea catheter were simple, safe and without being injured.
Xu
Dong
D
Department of Occupational Health and Toxicology, Zhengzhou University, Zhengzhou, 450052, China.
Wu
Yiming
Y
Wang
Peng
P
Xu
Yubao
Y
Meng
Aimin
A
Li
Hong
H
Liu
Zhangxian
Z
Zhu
Ran
R
D000818:Animals; D018893:Bronchoalveolar Lavage / Q000379:methods*; D003031:Coal; D004195:Disease Models, Animal; D004391:Dust; D008168:Lung / Q000473:pathology; D011009:Pneumoconiosis / Q000209:etiology*; D011817:Rabbits; D012822:Silicon Dioxide
D003031:Coal; D004391:Dust; D012822:Silicon Dioxide