Isolation of auxin efflux carrier cDNA from cucumber.
Cucumber seedlings display not only gravitropism but also peg formation in response to gravity. Gravimorphogenesis is mediated by auxin distribution. As first step to reveal the mechanism that regulates auxin distribution by auxin efflux, we isolated five partial cDNAs of auxin efflux carriers by RT-PCR method. In addition, we isolated two full-length cDNAs (CsPIN2, CsPIN3) from a cucumber cDNA library. CsPIN2, AtPIN3, AtPIN4 and AtPIN7 fall within the same clade. CsPIN3, AtPIN1 and CsPIN1 fall within the same clade. CsPIN5, CsPIN6 and AtPIN2 fall within the same clade. Our phylogenetic analysis of PIN in cucumber and Arabidopsis indicates that cucumber may diversify CsPIN protein compared with AtPIN protein.
Hotta
Takuya
T
Graduate School of Life Science, Tohoku University.
Kim
Dai-Hee
DH
Kamada
Motoshi
M
Fujii
Nobuharu
N
Kim
Kyung-Min
KM
Takahashi
Hideyuki
H
D029681:Arabidopsis Proteins; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D018553:Cucumis sativus / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant; D006112:Gravitation; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins; D010802:Phylogeny; D018514:Plant Structures / Q000254:growth & development
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis