Expression, purification, crystallization and preliminary crystallographic analysis of the diarrhoea-causing and virulence-determining region of rotaviral nonstructural protein NSP4.
The region spanning the tetrameric coiled-coil domain and the interspecies-variable virulence-determining region of the cytoplasmic tail of rotaviral nonstructural protein NSP4 has been crystallized. The crystals belong to space group I222, with unit-cell parameters a = 30.70, b = 38.07, c = 181.62 A, and contain two molecules in the asymmetric unit. Diffraction data have been collected utilizing a MAR imaging plate to a resolution of 2.2 A. The tetramer is generated by the crystallographic dyad along the c axis.
Deepa
R
R
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Jagannath
M R
MR
Kesavulu
M M
MM
Durga Rao
C
C
Suguna
K
K
D000818:Animals; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins; D037521:Virulence Factors