title: The genetic basis of a flower color polymorphism in the common morning glory (Ipomoea purpurea).
abstract: The common morning glory (Ipomoea purpurea) is highly polymorphic for flower color. Part of this phenotypic variation is due to allelic variation at the P locus. This locus determines whether flowers will be purple or pink, where purple is dominant to pink. We have determined that the anthocyanin biosynthetic gene flavonoid 3'-hydroxylase (f3'h) corresponds to the P locus. In the pink allele of f3'h there is a large insertion in the third exon, which results in the production of a truncated transcript. This shortened transcript produces a nonfunctional F3'H enzyme, resulting in the production of pink flowers rather than purple. In addition, we describe a polymerase chain reaction (PCR)-based assay that can be used to determine the genotype of a plant at this locus.
authors.affiliation: Biology Department, Duke University, Box 90338, Durham, NC 27708, USA. bzufall@smith.edu
authors.forename: R A
authors.initials: RA
authors.lastname: Zufall
authors.forename: M D
authors.initials: MD
authors.lastname: Rausher
mesh_terms: D001483:Base Sequence; D002855:Chromatography, Thin Layer; D020090:Chromosome Segregation / Q000235:genetics; D003116:Color; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D018744:DNA, Plant; D035264:Flowers / Q000235:genetics*; D005816:Genetic Complementation Test; D005838:Genotype; D027741:Ipomoea / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C038888:flavonoid 3'-hydroxylase