title: [Altered cholecystokinin-induced calcium signal in streptozotocin-induced diabetic rat pancreatic acini].
abstract: OBJECTIVE
  Pancreatic acini of streptozotocin (STZ)-induced diabetic rats release amylase less than normal acini on cholecystokinin (CCK) stimulation. Pancreatic enzyme secretion has been closely related to the intracellular calcium concentration ([Ca2(+)](i)) of the acinar cell. In the present study, sequential changes of the intracellular calcium signal which probably underlie the altered enzyme secretion in response to CCK-8 were investigated using pancreatic acini from diabetic rats.
  
  
  METHODS
  Diabetic rats were prepared by single intravenous injection of STZ (70 mg/kg). Stimulating experiments with CCK-8 were performed 7 days later. Pancreatic acini were isolated by collagenase digestion. Amylase release and [Ca2(+)](i) were measured by colorimethod and calcium imaging, respectively. The geometry of intracellular calcium signal was analyzed.
  
  
  RESULTS
  Normal acini exhibited concentration-dependent [Ca2(+)](i) increase and regular oscillatory calcium signal on CCK-8 stimulation. Amylase release was also concentration-dependent. However, diabetic acini showed significantly less [Ca2(+)](i) increase, prolonged time to peak [Ca2(+)](i), decreased calcium spikes number, and decreased amylase release compared with normal acini. The decreased [Ca2(+)](i) in diabetic acini was restored significantly by insulin treatment.
  
  
  CONCLUSIONS
  Relatively decreased amylase release in diabetic pancreatic acini in response to CCK, appears to be associated with altered calcium signal due to insulin deficiency.
authors.affiliation: Department of Physiology, The Catholic University of Korea College of Medicine, Socho-gu, Seoul, Korea.
authors.forename: Myung-Jun
authors.initials: MJ
authors.lastname: Kim
authors.forename: Gyeong-Ryul
authors.initials: GR
authors.lastname: Ryu
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Sung
authors.forename: Do Sik
authors.initials: DS
authors.lastname: Min
authors.forename: Duck-Joo
authors.initials: DJ
authors.lastname: Rhie
authors.forename: Shin Hee
authors.initials: SH
authors.lastname: Yoon
authors.forename: Sang June
authors.initials: SJ
authors.lastname: Hahn
authors.forename: Yang-Hyeok
authors.initials: YH
authors.lastname: Jo
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012844:Sincalide / Q000494:pharmacology*
chemical_list: D000681:Amylases; D012844:Sincalide