Esterified lipid hydroperoxide-derived modification of protein: formation of a carboxyalkylamide-type lysine adduct in human atherosclerotic lesions.
We have recently identified Nepsilon-azelayllysine (AZL) as a carboxyalkylamide-type novel lysine adduct in the reaction of linoleic acid hydroperoxides with the lysine derivative. To examine the formation of AZL in vivo, a novel monoclonal antibody (mAb19D5) specific to AZL moiety was prepared. The mAb19D5 scarcely recognized oxidized low-density lipoprotein (oxLDL), whereas the treatment of oxLDL with alkali or phospholipase A2 significantly increased the immunoreactivity. Similarly, the immunopositive materials were detected in alkali- or phospholipase A2-treated sections from human atherosclerotic aorta but not in untreated sections. These results suggest that esterified lipid hydroperoxide-derived modification of protein may serve as one mechanism for the oxidative modification of LDL and subsequent formation of atherosclerotic lesions in vivo.
Kawai
Yoshichika
Y
Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Fujii
Hiroyuki
H
Kato
Yoji
Y
Kodama
Michiteru
M
Naito
Michitaka
M
Uchida
Koji
K
Osawa
Toshihiko
T
D000468:Alkalies / Q000378:metabolism; D000577:Amides / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D001011:Aorta / Q000378:metabolism / Q000648:ultrastructure; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D003300:Copper / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D004951:Esterification; D006801:Humans; D007150:Immunohistochemistry; D008054:Lipid Peroxides / Q000737:chemistry* / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2
D000468:Alkalies; D000577:Amides; D000911:Antibodies, Monoclonal; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D003300:Copper; D010741:Phospholipases A; D054467:Phospholipases A2; D008239:Lysine