Mechanosensitive duodenal afferents contribute to vagal modulation of inflammation in the rat.
Noxious stimuli inhibit inflammation by activating neuroendocrine stress axes, an effect that is potently attenuated by ongoing activity in subdiaphragmatic vagal afferents. Because this vagal afferent activity is carried in the coeliac and coeliac accessory branches of the subdiaphragmatic vagus, we tested the hypothesis that the activity arises from vagal afferents that innervate a proximal segment of the gastrointestinal tract. Surgical removal of the duodenum, but not the stomach, produces a marked (six orders of magnitude) leftward shift in the dose-response curve for intraplantar capsaicin-induced inhibition of synovial plasma extravasation induced by the potent inflammatory mediator bradykinin, in the knee joint; this is similar in magnitude to the inhibition produced by subdiaphragmatic or by coeliac plus coeliac accessory branch vagotomy. Fasting, to unload mechanically sensitive polymodal afferents in the proximal gastrointestinal tract, produces a similar leftward shift in the dose-response curve for the inhibitory effect of capsaicin, an effect that is reversed by balloon distension in the duodenum in fasted rats, while balloon distension postvagotomy had no effect. These results suggest that activation of mechanically sensitive vagal afferents in the duodenum contributes vagal afferent activity that modulates neuroendocrine control of the inflammatory response.
Miao
Frederick Jia-Pei
FJ
NIH Pain Center, University of California at San Francisco, San Francisco, CA 94143-0440, USA.
Green
Paul G
PG
Levine
Jon D
JD
D000818:Animals; D001920:Bradykinin; D002211:Capsaicin / Q000494:pharmacology; D002404:Catheterization; D004386:Duodenum / Q000294:innervation* / Q000601:surgery; D004558:Electric Stimulation; D005215:Fasting / Q000502:physiology; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D007719:Knee Joint; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014628:Vagotomy; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
D002211:Capsaicin; D001920:Bradykinin