Expression of Helicobacter pylori Hsp60 protein and its immunogenicity.
OBJECTIVE
To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.


METHODS
Hsp60 DNA was amplified by PCR and inserted into the prokaryote expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal experiments.


RESULTS
DNA sequence analysis showed Hsp60 DNA was the same as GenBank's research. Hsp60 recombinant protein accounted for 27.2% of the total bacterial protein, and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 itself.


CONCLUSIONS
Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection.
Bai
Yang
Y
PLA Institute for Digestive Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Li
Liang-Ren
LR
Wang
Ji-De
JD
Chen
Ye
Y
Jin
Jian-Feng
JF
Zhang
Zhao-Shan
ZS
Zhou
Dian-Yuan
DY
Zhang
Ya-Li
YL
D000818:Animals; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology
D018834:Chaperonin 60; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins