title: A gene expressed exclusively in acute B lymphoblastic leukemias.
abstract: Representational difference analysis, a cDNA subtraction approach, was used to identify genes that are expressed in acute leukemia but not in normal hematological tissues. We isolated a cDNA fragment from a cell line derived from a B cell acute lymphoblastic leukemia bearing two Philadelphia chromosomes. The cDNA derives from an orphan gene that was named BLACE. BLACE is located in region 7q36 and encodes a major 5.3-kb transcript and several alternatively spliced minor transcripts. Significant expression of BLACE was detected by RT-PCR and quantitative RT-PCR in bone marrow samples from B cell acute lymphoblastic leukemia patients. BLACE was not or was scarcely expressed in other types of hematological malignancies, in normal tissues, and in solid tumors.
authors.affiliation: Laboratoire d'Immunologie des Tumeurs, Institut Paoli-Calmettes, Université de la Méditerranée, Marseille, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Vialle-Castellano
authors.forename: Sandra
authors.initials: S
authors.lastname: Laduron
authors.forename: Etienne
authors.initials: E
authors.lastname: De Plaen
authors.forename: Edgar
authors.initials: E
authors.lastname: Jost
authors.forename: Sophie
authors.initials: S
authors.lastname: Dupont
authors.forename: Geneviève
authors.initials: G
authors.lastname: Ameye
authors.forename: Lucienne
authors.initials: L
authors.lastname: Michaux
authors.forename: Pierre
authors.initials: P
authors.lastname: Coulie
authors.forename: Daniel
authors.initials: D
authors.lastname: Olive
authors.forename: Thierry
authors.initials: T
authors.lastname: Boon
authors.forename: Nicolas
authors.initials: N
authors.lastname: van Baren
mesh_terms: D017398:Alternative Splicing; D015152:Blotting, Northern; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA
chemical_list: C491840:BLACE lncRNA, human; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger