Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: a real time scanning tunnelling microscopy study.
A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C, which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in development of a bio-electrode surface has been predicted.
Mukhopadhyay
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New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QT, UK. rupam@iitk.ac.in
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D000327:Adsorption; D002021:Buffers; D004800:Enzymes, Immobilized; D005288:Ferredoxins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016958:Pseudomonas putida / Q000201:enzymology*
D002021:Buffers; D004800:Enzymes, Immobilized; D005288:Ferredoxins; C100137:putidaredoxin; D006046:Gold