Evidence for biological rhythm in spermatogenesis in the pubertal hamster (Mesocricetus auratus): a flow cytometric study.
The number of cells in the S-phase fraction of the cell cycle reflects proliferative activity. Using flow cytometry histograms and the Phoenix M+ cell cycle program, the percent of cells in the S-phase fraction was measured in single cell suspensions prepared from testes of hamsters of different ages. A cyclical pattern with a period of 9 days, superimposed on another rhythm with a 38 day period was observed (p < 0.01) during hamster maturation and it disappeared after the second spermatogenic wave, where the S phase values reached a plateau. It was concluded that maturing animals passed through a stage in which testicular biological rhythm was involved. Therefore it was concluded that it takes approximately two spermatogenic waves before the proliferation rate in the testis reached a steady state.
Vigodner
M
M
Department of Clinical Biochemistry, Sackler Medical School, Tel Aviv University, Ramat Aviv, 69978 Israel.
Lewy
H
H
Lewin
L M
LM
Shochat
L
L
Golan
R
R
D000367:Age Factors; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D006224:Cricetinae; D005434:Flow Cytometry; D008297:Male; D008647:Mesocricetus / Q000502:physiology*; D010507:Periodicity; D012741:Sexual Maturation / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000502:physiology