Sequence selective hydrolysis of linear DNA using conjugates of Zr(IV) complexes and peptide nucleic acids.
A series of conjugates of peptide nucleic acids (PNA) and Zr(IV) complexes was prepared. Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane, cleaves DNA targets sequence selectively in close proximity to the Zr(IV) complex. It was demonstrated that cleavage products are substrates of terminal transferase.
Zelder
Felix H
FH
Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Mokhir
Andriy A
AA
Krämer
Roland
R
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006868:Hydrolysis; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity; D015040:Zirconium / Q000737:chemistry*
D004277:DNA, Single-Stranded; D009942:Organometallic Compounds; D020135:Peptide Nucleic Acids; D015040:Zirconium