title: Application of solvent engineering to optimize lipase-catalyzed 1,3-diglyacylcerols by mixture response surface methodology.
abstract: 1,3-Diacylglycerol (DAG) has been introduced in Japan as a cooking oil under the trade name of Econa to reduce body fat accumulation. Solvent engineering was applied to determine the optimum solvent mixtures for the lipase-catalyzed synthesis of 1,3-DAG by mixture response surface methodology. n-Hexane was required to maintain the lipase activity and the product selectivity could be adjusted by changing the hydrophobicity of reaction medium. The optimum yield (approximately 40%) of 1,3-DAG synthesis was obtained with n-hexane/octane (1:1, v/v).
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, 45 Minsheng Road, Tamshui, Taipei County, 251, Taiwan.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Liao
authors.forename: Wei-Chuan
authors.initials: WC
authors.lastname: Tsai
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Chang
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
mesh_terms: D002384:Catalysis; D002616:Chemical Engineering / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D004075:Diglycerides / Q000138:chemical synthesis* / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D011786:Quality Control; D012997:Solvents / Q000737:chemistry*; D014304:Triolein / Q000737:chemistry*
chemical_list: D004075:Diglycerides; D006586:Hexanes; D012997:Solvents; D014304:Triolein; C026385:n-hexane; D008049:Lipase