[A study on the adduct characteristics of styrene and DNA].
OBJECTIVE
To explore the adduct characteristics of styrene and DNA.


METHODS
The adduct reactions between styrene, urinary mandalic acid(MA), phenylglyoxalic acid(PGA), mercapturic acid of styrene (UMA) and DNA were studied by ultraviolet spectral analysis. The SO-DNA adducts by 32P-post labeled method, the chemical structures of SO-DNA adducts by GC-MS and NMR were also studied.


RESULTS
SO combined with DNA at O6, N2 positions of dGMP to form six adducts, but styrene, urinary mandalic acid, phenylglyoxalic acid and mercapturic acid of styrene did not react with DNA to form adduct.


CONCLUSIONS
Styrene formed adduct with DNA through its active center metabolite--SO after entering the body. SO combined with DNA at O6, N2 positions of dGMP to form adducts. If these DNA adducts are not repaired or are mis-repaired before cell duplication, the gene mutation and chemical damage would happen. No adduct reactions are seen among other metabolites of styrene.
Shao
Hua
H
Shandong Institute of Labor Health and Occupational Disease, Jinan, Shandon 250062, China.
Li
Jie
J
Shi
Yikang
Y
D000111:Acetylcysteine / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism*; D004260:DNA Repair; D006038:Glyoxylates / Q000378:metabolism; D006801:Humans; D008333:Mandelic Acids / Q000378:metabolism; D020058:Styrene / Q000378:metabolism*
D018736:DNA Adducts; D006038:Glyoxylates; D008333:Mandelic Acids; C012482:phenylglyoxylic acid; D020058:Styrene; D004247:DNA; C037938:mandelic acid; D000111:Acetylcysteine