Defective RNA-mediated c-myc gene silencing pathway in Burkitt's lymphoma.
Keeping in view the fact that molecular basis of Burkitt's lymphoma (BL) is poorly understood, we attempted to explore the small interfering RNA (siRNA) mediated c-myc gene regulation using BL-derived EB-3 cell line as archetype cellular model. Such a study revealed that EB-3 cells possess 4-fold higher expression of Dicer gene coupled with 2-fold higher activity of RNA polymerase III than that observed in normal human lymphocytes. siRNAs derived from EB-3 cells had the inherent capacity to suppress c-myc gene expression in normal cells but not in native cells. Based on these findings we have proposed a novel RNA-mediated c-myc gene regulation pathway that may be responsible for BL.
Kaul
Deepak
D
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160 012, India. dkaul_24@hotmail.com
Sikand
Kavleen
K
D002051:Burkitt Lymphoma / Q000235:genetics*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D020868:Gene Silencing; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008954:Models, Biological; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012320:RNA Polymerase III / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D012313:RNA; D012320:RNA Polymerase III