Lack of genotoxicity of potassium iodate in the alkaline comet assay and in the cytokinesis-block micronucleus test. Comparison to potassium bromate.
Iodine could be added to the diet of human population in the form of iodide or iodate but iodate had not been adequately tested for genotoxicity and carcinogenicity. In the present study, genotoxic effects of potassium iodate were evaluated in vitro using the alkaline comet assay and the cytokinesis-block micronucleus assay on CHO cells and compared to halogenate salt analogues potassium bromate and chlorate and also to their respective reduced forms (potassium iodide, bromide and chloride). The results showed that the comet assay failed to detect the presence of DNA damage after a treatment of cells by potassium iodate for concentrations up to 10 mM. This absence of primary DNA damage was confirmed in the cytokinesis-block micronucleus assay. In the same way, results showed that potassium chlorate as well as potassium iodide, bromide and chloride did not induced DNA damage in the alkaline comet assay for doses up to 10 mM. By contrast, potassium bromate exposure led to an increase in both DNA damage and frequency of micronucleated cells. The repair of bromate-induced DNA damage was incomplete 24 h after the end of treatment. These results seem to indicate that potassium bromate would induce DNA damage by several mechanisms besides oxidative stress.
Poul
J M
JM
AFSSA, Laboratoire d'Etudes et de Recherches sur les Médicaments Vétérinaires et les Désinfectants, Unité de Toxicologie Alimentaire, BP 90203, 35302 Cedex Fougères, France. jm.poul@fougeres.afssa.fr
Huet
S
S
Godard
T
T
Sanders
P
P
D000818:Animals; D001959:Bromates / Q000008:administration & dosage / Q000633:toxicity*; D016466:CHO Cells / Q000187:drug effects; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007452:Iodates / Q000008:administration & dosage / Q000633:toxicity*; D015162:Micronucleus Tests; D017680:Potassium Compounds / Q000008:administration & dosage / Q000633:toxicity*
D001959:Bromates; D007452:Iodates; D017680:Potassium Compounds; C019536:potassium bromate; C039693:potassium iodate