Effect of complex amino acid imbalance on growth of tumor in tumor-bearing rats.
OBJECTIVE
To investigate the effect of complex amino acid imbalance on the growth of tumor in tumor-bearing (TB) rats.


METHODS
Sprague-Dawley (SD) rats underwent jejunostomy for nutritional support. A suspension of Walker-256 carcinosarcoma cells was subcutaneously inoculated. TB rats were randomly divided into groups A, B, C and D according to the formula of amino acids in enteral nutritional solutions, respectively. TB rats received jejunal feedings supplemented with balanced amino acids (group A), methionine-depleted amino acids (group B), valine-depleted amino acids (group C) and methionine- and valine-depleted complex amino acid imbalance (group D) for 10 days. Tumor volume, inhibitory rates of tumor, cell cycle and life span of TB rats were investigated.


RESULTS
The G0/G1 ratio of tumor cells in group D (80.5 +/- 9.0)% was higher than that in groups A, B and C which was 67.0 +/- 5.1%, 78.9 +/- 8.5%, 69.2 +/- 6.2%, respectively (P<0.05). The ratio of S/G2M and PI in group D were lower than those in groups A, B and C. The inhibitory rate of tumor in groups B, C and D was 37.2%, 33.3% and 43.9%, respectively (P<0.05). The life span of TB rats in group D was significantly longer than that in groups B, C, and A.


CONCLUSIONS
Methionine/valine-depleted amino acid imbalance can inhibit tumor growth. Complex amino acids of methionine and valine depleted imbalance have stronger inhibitory effects on tumor growth.
He
Yin-Cheng
YC
Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China. w030508h@public.wh.hb.cn
Wang
Yuan-Hong
YH
Cao
Jun
J
Chen
Ji-Wei
JW
Pan
Ding-Yu
DY
Zhou
Ya-Kui
YK
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002279:Carcinoma 256, Walker / Q000473:pathology*; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D007582:Jejunostomy; D008017:Life Expectancy; D018529:Nutritional Support; D051381:Rats; D017207:Rats, Sprague-Dawley
D000596:Amino Acids