title: Purification and crystallization of haemoglobin from donkey (Equus asinus).
abstract: Haemoglobin acts as an important protein for oxygen carrier in all living beings. Purification of donkey haemoglobin was done using PEG 4000 as a separating medium. Crystallization was achieved using hanging drop vapor diffusion method using 2.8 M phosphate buffer, pH 6.8. Data collection was done using mar345 image plate detector system. The crystals grown under 2.8 M phosphate buffer are monoclinic with space group C2 and cell dimensions a=107.664 A, b=63.084 A, c=54.042 A, and beta=111.747 degrees.
authors.affiliation: Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India.
authors.forename: D
authors.initials: D
authors.lastname: Balasundaresan
authors.forename: K
authors.initials: K
authors.lastname: Saraboji
authors.forename: M N
authors.initials: MN
authors.lastname: Ponnuswamy
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods*; D018397:Equidae; D006454:Hemoglobins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013329:Structure-Activity Relationship
chemical_list: D006454:Hemoglobins