title: The role of glutathione in yeast dehydration tolerance.
abstract: Among the factors that affect cell resistance against dehydration, oxidation is considered to be of great importance. In this work, we verified that both control and glutathione deficient mutant strains were much more oxidized after dehydration. Moreover, cells lacking glutathione showed a twofold higher increase in oxidation and lipid peroxidation than the control strain. While glucose 6-phosphate dehydrogenase and glutathione reductase activities did not change in response to dehydration in the control strain, the mutant strain gsh1 (glutathione deficient) showed a reduction of 50% in both activities, which could explain the high levels of oxidation shown by gsh1 cells. In conformity with these results, the mutant lacking GSH1 showed a high sensitivity to dehydration. Furthermore, the addition of glutathione to gsh1 cells restored survival rates to the levels of the control strain. We conclude that glutathione plays a significant role in the maintenance of intracellular redox balance during dehydration.
authors.affiliation: Departamento Bioqui;mica, Instituto de Química, UFRJ, Rio de Janeiro, Brazil.
authors.forename: Aline de Souza
authors.initials: Ade S
authors.lastname: Espindola
authors.forename: Débora Silva
authors.initials: DS
authors.lastname: Gomes
authors.forename: Anita Dolly
authors.initials: AD
authors.lastname: Panek
authors.forename: Elis Cristina Araujo
authors.initials: EC
authors.lastname: Eleutherio
mesh_terms: D003681:Dehydration / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D005978:Glutathione