Ultracompact autocorrelator for multiphoton microscopy.
Pulse temporal characterization is a fundamental task when operating a Ti:Sapphire ultrafast laser system for multiphoton microscopy applications. In the present report, an ultracompact autocorrelator setup and a simple procedure is reported to perform pulse width measurements at the focal plane of the microscope objective without the need of any further instrumentation, aside from a few optical elements, since the confocal microscope, detection, data acquisition, processing, and displaying capabilities are used.
Quercioli
F
F
Istituto Nazionale di Ottica Applicata, Biophotonics Laboratory, Largo Enrico Fermi 6, 50125 Firenze, Italy. quercioli@inoa.it
Ghirelli
A
A
Tiribilli
B
B
Vassalli
M
M
D004867:Equipment Design; D007368:Interferometry / Q000295:instrumentation; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D017785:Photons; D012680:Sensitivity and Specificity; D013997:Time Factors