Absence of correlation between glycated hemoglobin and lipid composition of erythrocyte membrane in type 2 diabetic patients.
Correlation of glycated hemoglobin (HbA(1c)) level with degrees of certain peroxidative changes in erythrocyte membrane lipids in diabetic patients have been reported. In the present study, peroxidation of erythrocyte lipids was assessed by changes in tocopherols (Toc), phospholipids (PL), and malondialdehyde (MDA). Membrane cholesterol, Toc, and PL were determined from the same lipid extract. Toc and cholesterol were measured simultaneously by high-performance liquid chromatography (HPLC), and each PL class was determined by a single HPLC elution with ultraviolet light (UV) detection. The detection of PL with UV depends primarily on double bonds in fatty acids and shows a decrease in fatty acids by peroxidation. Changes in Toc and each PL were calculated on the basis of cholesterol and SM, respectively, since cholesterol and sphingomyelin (SM) in the cell membrane are not prone to peroxidation. MDA was measured by an HPLC method with fluorescence detection. These methods for assessment for peroxidation of membrane lipids in intact erythrocytes were validated by experiments with 2, 2-azobis(2-amidinopropane)dihydrochloride (AAPH) and tert-butylhydroperoxide (tBHP); nevertheless, significant differences in the levels of Toc, each PL class, and MDA between a high-HbA(1c) group and a low-HbA(1c) group were not detected.
Mawatari
Shiro
S
Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women's University, Japan.
Saito
Kazuyuki
K
Murakami
Kaori
K
Fujino
Takehiko
T
D000328:Adult; D016718:Arachidonic Acid / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004281:Docosahexaenoic Acids / Q000097:blood; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005227:Fatty Acids / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010714:Phosphatidylethanolamines / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D010743:Phospholipids / Q000097:blood; D013109:Sphingomyelins / Q000097:blood; D024505:Tocopherols / Q000097:blood; D024502:alpha-Tocopherol / Q000097:blood; D024504:gamma-Tocopherol / Q000097:blood; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D016718:Arachidonic Acid; D002784:Cholesterol; D004281:Docosahexaenoic Acids; D005227:Fatty Acids; D006442:Glycated Hemoglobin; D008055:Lipids; D008315:Malondialdehyde; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D013109:Sphingomyelins; D024505:Tocopherols; D024502:alpha-Tocopherol; D024504:gamma-Tocopherol; D020122:tert-Butylhydroperoxide