title: Real-time nucleic acid sequence-based amplification in nanoliter volumes.
abstract: Real-time nucleic acid sequence-based amplification (NASBA) is an isothermal method specifically designed for amplification of RNA. Fluorescent molecular beacon probes enable real-time monitoring of the amplification process. Successful identification, utilizing the real-time NASBA technology, was performed on a microchip with oligonucleotides at a concentration of 1.0 and 0.1 microM, in 10- and 50-nL reaction chambers, respectively. The microchip was developed in a silicon-glass structure. An instrument providing thermal control and an optical detection system was built for amplification readout. Experimental results demonstrate distinct amplification processes. Miniaturized real-time NASBA in microchips makes high-throughput diagnostics of bacteria, viruses, and cancer markers possible, at reduced cost and without contamination.
authors.affiliation: NorChip AS, Industriveien 8, 3490 Klokkarstua, Norway. Anja.Gulliksen@norchip.com
authors.forename: Anja
authors.initials: A
authors.lastname: Gulliksen
authors.forename: Lars
authors.initials: L
authors.lastname: Solli
authors.forename: Frank
authors.initials: F
authors.lastname: Karlsen
authors.forename: Henrik
authors.initials: H
authors.lastname: Rogne
authors.forename: Eivind
authors.initials: E
authors.lastname: Hovig
authors.forename: Trine
authors.initials: T
authors.lastname: Nordstrøm
authors.forename: Reidun
authors.initials: R
authors.lastname: Sirevåg
mesh_terms: D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D021161:Self-Sustained Sequence Replication / Q000379:methods*