Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase.
Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4 degrees C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.
Song
Aimin
A
Division of Hematology and Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California-Davis, 4501 X Street, Sacramento, CA 95817, USA.
Wang
Xiaobing
X
Zhang
Jinhua
J
Marík
Jan
J
Lebrilla
Carlito B
CB
Lam
Kit S
KS
D019921:Biotinylation; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides