Differences in the chemical coding of nerve fibres supplying major populations of neurons between the caudal mesenteric ganglion and anterior pelvic ganglion in the male pig.
The present study was designed to investigate and to compare the chemical coding of nerve fibres supplying major populations of neurons in the caudal mesenteric (CaMG) and anterior pelvic (APG) ganglion in juvenile male pigs (n=5) using double-labelling immunofluorescence. The co-existence patterns of some biologically active substances including tyrosine hydroxylase (TH) and vesicular acetylcholine transporter (VAChT) as well as vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP), Leu5-enkephalin (LENK) and serotonin (5-HT) were analysed under a confocal laser scanning microscope. Profound differences in the neurochemical features of the nerve terminals between the ganglia were observed. Moreover, there were also distinct differences in the chemical coding of nerve fibres associated with the particular populations and subpopulations of neurons within the ganglia. In both ganglia, nearly all adrenergic and cholinergic neurons were supplied with VAChT-positive nerve fibres (putative preganglionic fibres). However, in the CaMG, they were more numerous and, in contrast to the APG, many of them also stained for VIP. In the APG, a great number of nerve terminals expressed immunoreactivity to SP and CGRP (putative collaterals of sensory neurons). Interestingly, they densely supplied almost exclusively adrenergic neurons. SP-positive nerve fibres were moderate in number in the CaMG, but, in addition to VAChT-IR nerve terminals, the most numerous populations of nerve fibres in this ganglion were those expressing highly colocalized immunoreactivities to CGRP and LENK, and those which stained for 5-HT (putative processes of enteric neurons). However, these fibres supplied almost exclusively larger, intensely stained for TH and clustered adrenergic neurons. This diversity of the nerve terminals reflects the complexity of nerve circuits involved in the innervation of structures supplied by neurons in the porcine CaMG and APG. It also demonstrates the importance of nerve inputs for the proper function of autonomic neurons and thus their target tissues.
Kaleczyc
Jerzy
J
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland. jerzyk@uwm.edu.pl
Sienkiewicz
Waldemar
W
Klimczuk
Magdalena
M
Czaja
Krzysztof
K
Lakomy
Mirosław
M
D000818:Animals; D017776:Autonomic Pathways / Q000166:cytology* / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005725:Ganglia, Autonomic / Q000166:cytology* / Q000378:metabolism; D007001:Hypogastric Plexus / Q000166:cytology* / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D034421:Sus scrofa / Q000033:anatomy & histology* / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D014781:Viscera / Q000294:innervation / Q000502:physiology
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D018377:Neurotransmitter Agents; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D012701:Serotonin; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004743:Enkephalin, Leucine; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide