title: Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.
abstract: CHL27, the Arabidopsis homologue to Chlamydomonas Crd1, a plastid-localized putative diiron protein, is required for the synthesis of protochlorophyllide and therefore is a candidate subunit of the aerobic cyclase in chlorophyll biosynthesis. delta-Aminolevulinic acid-fed antisense Arabidopsis plants with reduced amounts of Crd1/CHL27 accumulate Mg-protoporphyrin IX monomethyl ester, the substrate of the cyclase reaction. Mutant plants have chlorotic leaves with reduced abundance of all chlorophyll proteins. Fractionation of Arabidopsis chloroplast membranes shows that Crd1/CHL27 is equally distributed on a membrane-weight basis in the thylakoid and inner-envelope membranes.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Box 951569, Los Angeles, CA 90095, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tottey
authors.forename: Maryse A
authors.initials: MA
authors.lastname: Block
authors.forename: Michael
authors.initials: M
authors.lastname: Allen
authors.forename: Tomas
authors.initials: T
authors.lastname: Westergren
authors.forename: Catherine
authors.initials: C
authors.lastname: Albrieux
authors.forename: Henrik V
authors.initials: HV
authors.lastname: Scheller
authors.forename: Sabeeha
authors.initials: S
authors.lastname: Merchant
authors.forename: Poul Erik
authors.initials: PE
authors.lastname: Jensen
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D015388:Organelles / Q000187:drug effects / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011521:Protochlorophyllide / Q000096:biosynthesis*; D020524:Thylakoids / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D011521:Protochlorophyllide; D000622:Aminolevulinic Acid; D010088:Oxidoreductases