title: Synergistic effect of CMP/KDO synthase inhibitors with antimicrobial agents on inhibition of production and release of Vero toxin by enterohaemorrhagic Escherichia coli O157:H7.
abstract: Synergistic effect of CMP/KDO synthase inhibitors in LPS biosynthesis of Gram-negative bacteria with kanamycin (KM) and fosfomycin (FOM) on the production and release of Vero toxins (VTs) by Escherichia coli O157 was evaluated in vitro. While CMP/KDO synthase inhibitors, KM and FOM showed no inhibitory effect on the production/release of VTs by themselves alone, both KM and FOM showed the remarkable inhibition of VT2 release through synergistic collaboration with CMP:KDO synthase inhibitor.
authors.affiliation: Microbial Chemistry Research Center, 3-14-23 Kamiosaki, Shinagawa-ku, 141-0021, Tokyo, Japan.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Kondo
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Doi
authors.forename: Hayamitsu
authors.initials: H
authors.lastname: Adachi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nishimura
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000201:enzymology; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D022603:Shiga Toxins / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D022603:Shiga Toxins; D009713:Nucleotidyltransferases; C028984:3-deoxy-manno-octulosonate cytidyltransferase