Interaction assay of oligosaccharide with lectins using glycosylasparagine.
Glycosyl amino acids having natural glycan were useful for the interaction assay of oligosaccharides. A glycochip containing the whole structure of an oligosaccharide was easily prepared by the immobilization of the glycosyl amino acid. Furthermore, fluorescence probes were introduced into the glycosyl amino acid while maintaining the whole structure of oligosaccharide. By using these labeled oligosaccharides, fluorescence polarization (FP) and fluorescence correlation spectroscopy (FCS) analyzed the carbohydrate-lectin interaction in a solution assay system.
Mizuno
Mamoru
M
The Noguchi Institute, 1-8-1, Kaga, Itabashi-ku, 173-0003, Tokyo, Japan. mmizuno@noguchi.or.jp
Noguchi
Midori
M
Imai
Mie
M
Motoyoshi
Tetsuya
T
Inazu
Toshiyuki
T
D000818:Animals; D001216:Asparagine / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005260:Female; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology
D037102:Lectins; D009844:Oligosaccharides; D001216:Asparagine