title: Differential prevalence of HIV type 1 subtype B and CRF01_AE among different sexual transmission groups in Tokyo, Japan, as revealed by subtype-specific PCR.
abstract: We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain re-action (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRFO1_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype Band 12 with CRFO1_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRFO1_AE are evenly predominant in a heterosexually transmitted group.
authors.affiliation: Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. skato@sc.itc.keio.ac.jp
authors.forename: Shingo
authors.initials: S
authors.lastname: Kato
authors.forename: Rie
authors.initials: R
authors.lastname: Saito
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hiraishi
authors.forename: Naoto
authors.initials: N
authors.lastname: Kitamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Hideji
authors.initials: H
authors.lastname: Hanabusa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kamakura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Negishi
mesh_terms: D005260:Female; D015658:HIV Infections / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020010:Heterosexuality; D006716:Homosexuality; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011159:Population Surveillance; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014041:Tokyo