[Effect of acrylamide on creatine kinase and adenosine triphosphate in brain of mice and its significance].
OBJECTIVE
To explore the changes of brain energy metabolism following acrylamide (ACR) poisoning.


METHODS
Creatie kinase (CK), adenosine triphosphate (ATP), adenosine diphosphate(ADP), adenosine 5'-monophosphate(AMP) and glucose contents in brain were observed in O1a mice and 6J mice following ACR intoxication by enzyme analytical method.


RESULTS
ATP, CK and glucose levels decreased transiently in O1a mice, while ATP level in 6J mice was significantly decreased (1.76 mumol/g, P < 0.01), as compared to the control (2.53 mumol/g) but ADP and AMP were increased, glucose was decreased. The activity of CK in poisoned group (1.13 mumol/g, P < 0.01) was lower than that of control (3.16 mumol/g and lasted for 5 weeks).


CONCLUSIONS
The influence of ACR on O1a mice was slight and reversible but on 6J mice was severe and lasting. There was severe damage to the potential energy supply compensation, which might be the biochemical basis of neuron damage induced by acrylamide.
He
Qiuyue
Q
Department of Neurology, Shenzhen Red Cross Hospital, Shenzhen 518035, China.
Han
Manfu
M
Rao
Mingli
M
D020106:Acrylamide / Q000506:poisoning*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003402:Creatine Kinase / Q000032:analysis*; D004734:Energy Metabolism / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D051379:Mice
D020106:Acrylamide; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D005947:Glucose