title: Effects of different acid and sodium hypochlorite treatments on dentin collagen: a FEISEM analysis.
abstract: OBJECTIVE
  To evaluate if different acid treatments of dentin surface might remove different amount of mineralized dentin, thus exposing and modifying the CF network.
  
  
  METHODS
  Dentin disks prepared from human third molars with a low-speed diamond saw were etched for 15 seconds with the tested acids: citric acid 10%, maleic acid 10%, 2.5% oxalic acid, 35% phosphoric acid and 24% EDTA gel. Specimens were then submitted to a 5% solution of NaOCl for 2 minutes or 5 minutes, fixed, and observed utilizing a field emission in lens SEM (FEISEM). Control specimens were also prepared by omitting the etching agent and/or the NaOCl solution.
  
  
  RESULTS
  The different acid treatments created specific dentin morphological pattern. CF exposure was in relationship with the acid used. The NaOCl solution greatly affects the acid etched dentin by removing the CF, nevertheless the effect of NaOCl was greatly influenced by the previous acid treatment.
authors.affiliation: Clinica Odontostomatologica, Department of Surgical Sciences, University of Trieste, Trieste, Italy. lbreschi@units.it
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
authors.forename: Pietro
authors.initials: P
authors.lastname: Gobbi
authors.forename: Stefano
authors.initials: S
authors.lastname: Chersoni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mazzotti
authors.forename: Carlo
authors.initials: C
authors.lastname: Prati
mesh_terms: D000134:Acid Etching, Dental; D002614:Chelating Agents / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008298:Maleates / Q000494:pharmacology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019815:Oxalic Acid / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D010756:Phosphoric Acids / Q000494:pharmacology; D019163:Reducing Agents / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D008298:Maleates; D016877:Oxidants; D010756:Phosphoric Acids; D019163:Reducing Agents; D019343:Citric Acid; D003094:Collagen; C030272:maleic acid; D019815:Oxalic Acid; D004492:Edetic Acid; D012973:Sodium Hypochlorite