[Studies on dihydrotestosterone-binding capacity in prostate tissue].
OBJECTIVE
To explore the effect of the binding ability of the dihydrotestosterone(DHT) in prostate.


METHODS
Twenty-two normal prostate tissues taken from accident-death corpses without serious diseases, and cytosolic and nuclear fractions were prepared with all the endogenous hormone removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed by adding 3H-DHT.


RESULTS
The average DHT-binding capacity of the DHT-binding protein in prostate was (0.0263 +/- 0.0047) nmol/g wet tissue. The DHT-binding capacities of cytosolic and nuclear fractions were (0.0103 +/- 0.0015) nmol/g wet tissue and (0.0155 +/- 0.0035) nmol/g wet tissue respectively, and the difference between them was very significant(P < 0.01).


CONCLUSIONS
The DHT-binding capacity of the DHT-binding protein in prostate is high and maintaining the high DHT level facilitates the effect of DHT.
Jiang
Hong-Tao
HT
Department of Urology, Shenzhen People's Hospital, Medical College of Jinan University, Shenzhen, Guangdong 518020, China.
Chen
Zhao-Dian
ZD
D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding
D013196:Dihydrotestosterone