[Establishment of transgenic mice for HRX-EEN fusion gene].
OBJECTIVE
To study the biological function of fusion gene HRX-EEN and its role in leukemogenesis, and to provide an ideal animal model for anti-leukemia drug screening.


METHODS
HRX-EEN fusion gene was constructed by use of three different DNA fragments, and it was inserted into hCG transgenic vector. G(0) transgenic mice were obtained by microinjection of the recombined DNA into the pronucleus of zygotes, followed by implantation of the injected zygotes into pseudopregnant mice. The integration of the transgene was tested by PCR and its expression by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The sequence of recombined HRX-EEN gene was confirmed by sequencing. PCR testing revealed a total of 7 G(0) transgenic mice, these mice were then mated with C57 wild type mice. Except mouse No. 35 that died, the others all had their F1 offsprings. From these 6 lines of transgenic mice, HRX-EEN gene was found to be stably expressed in 3 lines by RT-PCR. Up to now, all transgenic mice expressing the fusion gene have no obvious abnormal phenotypes.


CONCLUSIONS
A transgenic mice model in which the HRX-EEN fusion gene can be stably expressed has been established.
Sun
Yue-ping
YP
Department of Medical Genetics, Shanghai Hematology Institute, Shanghai Second Medical University, Shanghai, 200025 PR China.
Xiong
Hui
H
Wang
Yang
Y
Wang
Long
L
Huang
Qiu-hua
QH
Zhang
Qing-hua
QH
Kong
Hui
H
Zhang
Li-jun
LJ
Chen
Sai-juan
SJ
Chen
Zhu
Z
Wang
Zhu-gang
ZG
Lu
Zhen-yu
ZY
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008822:Mice, Transgenic; D051788:Myeloid-Lymphoid Leukemia Protein; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C105159:SH3GL1 protein, human; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase; C497423:Kmt2a protein, mouse