title: Cell-permeable small molecule probes for site-specific labeling of proteins.
abstract: We have successfully synthesized a number of small molecule probes designed for site-specific labeling of N-terminal cysteine-containing proteins expressed in live cells. Their utility for site-specific, covalent modifications of proteins was successfully demonstrated with purified proteins in vitro, and with live bacterial cells in vivo.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Dawn S Y
authors.initials: DS
authors.lastname: Yeo
authors.forename: Rajavel
authors.initials: R
authors.lastname: Srinivasan
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D002463:Cell Membrane Permeability; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005786:Gene Expression Regulation; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins; D003545:Cysteine