The effects of lectins on indomethacin-induced small intestinal ulceration.
Growth factors, such as epidermal growth factor and keratinocyte growth factor, have considerable therapeutic potential for repairing mucosal injury in the intestine when given systemically. Recently, several lectins have been shown to have trophic effects on the intestine when given orally. We examined the effects of phytohaemagglutinin (PHA) and concanavalin A (Con-A) on indomethacin-induced intestinal injury in rat. Five-week-old rats were randomized to four groups (n=5), and intestinal injury was induced by indomethacin injection in three of these groups. Elemental diet (ED) feeding was then commenced. The groups were thus ED feeding/indomethacin untreated (control group), ED feeding/indomethacin treated (ED group), 0.1% PHA-supplemented ED feeding/indomethacin treated (PHA group) and 0.1% Con-A-supplemented ED feeding/indomethacin treated (Con-A group). After 7 days of feeding, macroscopic inflammatory scores, mucosal permeability, myeloperoxidase (MPO) activities and cell proliferation were determined. Macroscopic inflammatory scores, mucosal permeability and MPO activities were significantly lower in both lectin groups than that in control group. Twenty-four hour excretion rate of phenolsulphonphthalein was significantly lower in both lectin groups than that in ED group. Cell proliferation of the small intestine was significantly increased by both lectins. Lectin supplementation can induce ulcer healing following indomethacin-induced damage.
Yasuoka
Takashi
T
Department of Gastroenterology, Shiga University of Medical Science, Otsu, Japan. yasuoka@belle.shiga-med.ac.jp
Sasaki
Masaya
M
Fukunaga
Tetsuya
T
Tsujikawa
Tomoyuki
T
Fujiyama
Yoshihide
Y
Kushima
Ryouji
R
Goodlad
Robert A
RA
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000627:therapeutic use; D005526:Food, Formulated; D007213:Indomethacin; D007410:Intestinal Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects; D037102:Lectins / Q000627:therapeutic use*; D008297:Male; D010539:Permeability / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010835:Phytohemagglutinins / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014456:Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
D037102:Lectins; D010835:Phytohemagglutinins; D003208:Concanavalin A; D009195:Peroxidase; D007213:Indomethacin