[Genetic analysis of Yersinia pestis strains isolated in China].
OBJECTIVE
The strains of Yersinia pestis isolated in different period and different natural foci in China were analyzed.


METHODS
Traditional and molecular biological methods were used. Rhamnose fermentation, rRNA gene copy number, nitrite reduction, and the glycerol fermentation were important characters for typing, and pulse field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) profile could reflect the genetic distance between the strains.


RESULTS
The strains could be divided into 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.


CONCLUSIONS
The characters of strains were described; the genetic relationship of different types, their evolution, and the forming and shift of plague natural foci were analyzed.
Yu
Dong-zheng
DZ
Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Hai
Rong
R
Dong
Xing-qi
XQ
Li
Min
M
Xia
Lian-xu
LX
Shi
Xian-ming
XM
Wei
Jian-chun
JC
Cui
Bai-zhong
BZ
Wang
Peng
P
Sun
Lian-zhi
LZ
Zhang
Zhi-kai
ZK
Hu
Yuan
Y
Zhang
En-min
EM
D002681:China; D030541:Databases, Genetic; D016521:Electrophoresis, Gel, Pulsed-Field; D040961:Genetic Drift; D005843:Geography; D009154:Mutation; D019105:Random Amplified Polymorphic DNA Technique; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification