A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor.
Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster.
Li
Yong-quan
YQ
College of Life Science, Zhejiang University, Hongzhou 310027, China. lyq@m.hz.zj.cn
Chen
Pei-lin
PL
Chen
Shi-fei
SF
Wu
Dan
D
Zheng
Jing
J
D000880:Anthraquinones / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005809:Genes, Regulator / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D011353:Prodigiosin; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones