Induction of protective immunity by primed B-1 cells in Toxoplasma gondii -infected B cell-deficient mice.
We examined the role of B-1 cells in protection against Toxoplasma gondii infection using B cell-deficient mice (muMT mice). We found that primed but not naïve B-1 cells from wild-type C57BL/6 mice protected B cell-deficient recipients from challenge infection. All muMT mice transferred with primed B-1 cells survived more than 5 months after T. gondii infection, whereas 100% of muMT mice transferred with naïve B-1 cells succumbed by 18 days after infection. Additionally, high expression of both T help (Th) 1- and Th2-type cytokines and a high level of nitric oxide production were observed in T. gondii-infected muMT mice transferred with primed B-1 cells. Thus, it was clearly demonstrated that B-1 cells play an important role in host protection against T. gondii infection in muMT mice.
Chen
Mei
M
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Inohana, Chiba, Japan.
Mun
Hye-Seong
HS
Piao
Lian-Xun
LX
Aosai
Fumie
F
Norose
Kazumi
K
Mohamed
Rabie M
RM
Belal
Usama S
US
Fang
Hao
H
Ahmed
Azza K
AK
Kang
Hyun-Kyu
HK
Matsuzaki
Goro
G
Kitamura
Daisuke
D
Yano
Akihiko
A
D019264:Adoptive Transfer; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D018664:Interleukin-12 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology; D016019:Survival Analysis; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000469:parasitology*
D016207:Cytokines; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D009569:Nitric Oxide; D007371:Interferon-gamma