Photothermal detection of laser-induced damage in single intact cells.
OBJECTIVE
Most of the studies of laser-induced damage do not analyze individual cells. Objective of this work was to evaluate local photo-induced thermal phenomena in single cells at theoretical and experimental levels for developing the method for real-time detection of laser damage in intact cells.


METHODS
Theoretical model of cell-laser interaction assumes local nature of photo-induced thermal effects and describes photodamage through bubble formation. Photothermal (PT) method was suggested for damage detection. Laser-induced damage was verified for individual cells with two techniques through detection of Trypan blue penetration into damaged cell.


RESULTS
Specific PT responses from blast-transformed lymphocytes were identified independently as result of bubble formation and cell damage. Probability of cell damage was obtained for cells as function of laser pulse energy.


CONCLUSIONS
The Laser load test (LLT) was suggested for real-time detection of damage, damage threshold measurement, and investigation of intact single cells.
Lapotko
Dmitri O
DO
International Center, Luikov Heat and Mass Transfer Institute, Minsk, 220072, Belarus. ld@hmti.ac.by
Lukianova
Ekaterina Y
EY
Shnip
Alexander I
AI
D002461:Cell Line, Transformed; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D007834:Lasers / Q000009:adverse effects*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000528:radiation effects*; D008954:Models, Biological; D010782:Photolysis