[Detection and significance of succinate dehydrogenase of sperm mitochondria].
OBJECTIVE
To detect succinate dehydrogenase (SDH) of sperm mitochondria by an improved method, and to evaluate the relationship between SDH and the motility and viability of sperm.


METHODS
In 46 fertile and infertile men (aged from 25 to 36), the motility, viability and mitochondrial SDH of sperm were detected by computer-assisted semen analysis system, dead/live sperm molecular fluorescent probe and an improved cytochemical method. Then, the correlation between the motility and viability of sperm and the positive percentage of sperm mitochondrial SDH were analyzed.


RESULTS
In the 46 fertile and infertile men, the motility and viability of sperm were (67.33 +/- 7.37)% and (79.78 +/- 7.65)%, and the positive percentage of sperm mitochondrial SDH was (74.74 +/- 8.29)%. The motility and viability of sperm and positive percentage of sperm mitochondrial SDH had significant correlation (r = 0.901, P < 0.01; r = 0.876, P < 0.01; r = 0.917, P < 0.01).


CONCLUSIONS
Detection of sperm mitochondrial SDH has significance in evaluation of sperm mitochondrial function and may serve as an assisted marker of sperm viability.
Xue
Xiao-Ping
XP
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Shang
Xue-Jun
XJ
Fu
Jian
J
Chen
Yong-Gang
YG
Shi
Yi-Chao
YC
D000328:Adult; D016022:Case-Control Studies; D002470:Cell Survival; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007248:Infertility, Male / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D013385:Succinate Dehydrogenase