Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model.
Recombinant Bombyx mori nucleopolyhedroviruses (BmNPV) displaying the immunodominant ectodomains of fusion glycoprotein (F) of Peste des petitis ruminants virus (PPRV) and the hemagglutinin protein (H) of Rinderpest virus (RPV), on the budded virions as well as the surface of the infected host cells have been constructed. The F and H protein sequences were inserted in-frame within the amino-terminal region of BmNPV envelope glycoprotein GP64 expressing under the strong viral polyhedrin (polh) promoter. We improved the recombinant virus selection in BmNPV by incorporating the green fluorescent protein gene (gfp) as selection marker under a separate promoter within the transfer cassette harboring the desired genes. Following infection of the insect larvae or the host-derived BmN cells with these recombinant BmNPVs, the expressed GP64 fusion proteins were displayed on the host cell surface and the budded virions. The antigenic epitopes of the recombinant proteins were properly displayed and the recombinant virus particles induced immune response in mice against PPRV or RPV.
Rahman
Md Masmudur
MM
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Shaila
M S
MS
Gopinathan
Karumathil P
KP
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D012831:Bombyx / Q000821:virology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006389:Hemagglutinins, Viral; D007114:Immunization; D007814:Larva / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism*; D012302:Rinderpest virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
D000914:Antibodies, Viral; C096524:F protein, Peste-des-petits-ruminants virus; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C079749:hemagglutinin protein, Rinderpest virus