title: Purification, crystallization and preliminary X-ray diffraction studies on pyruvate phosphate dikinase from maize.
abstract: Pyruvate phosphate dikinase (PPDK) from maize catalyzes the reversible conversion of ATP, orthophosphate and pyruvate to AMP, pyrophosphate and PEP. In higher plants, this enzyme is believed to be involved in the C(4) dicarboxylic acid pathway. PPDK was crystallized by the vapour-diffusion method using polyethylene glycol as a precipitant. The crystals belong to the orthorhombic space group C2, with unit-cell parameters a = 108.2, b = 100.2, c = 108.4 A, beta = 96.5 degrees, and diffract to 2.3 A using SPring-8 synchrotron radiation.
authors.affiliation: Kinetic Crystallography Research Team, RIKEN Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan.
authors.forename: Tsugumi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohki
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Oda
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Kanzo
authors.initials: K
authors.lastname: Sakata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kato
mesh_terms: D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D011772:Pyruvate, Orthophosphate Dikinase / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics
chemical_list: D018744:DNA, Plant; D011994:Recombinant Proteins; D011772:Pyruvate, Orthophosphate Dikinase