An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans.
In the nematode Caenorhabditis elegans, the gonad acquires two U-shaped arms through the directed migration of its distal tip cells (DTCs), which are located at the tip of the growing gonad arms. A member of the ADAM (a disintegrin and metalloprotease) family, MIG-17, regulates directional migration of DTCs: MIG-17 is synthesized and secreted from the muscle cells of the body wall, and diffuses to the gonad where it is required for DTC migration. The mig-23 mutation causes defective migration of DTCs and interacts genetically with mig-17. Here, we report that mig-23 encodes a membrane-bound nucleoside diphosphatase (NDPase) required for glycosylation and proper localization of MIG-17. Our findings indicate that an NDPase affects organ morphogenesis through glycosylation of the MIG-17 ADAM protease.
Nishiwaki
Kiyoji
K
RIKEN Center for Developmental Biology and PRESTO, Japan Science and Technology Corporation, Chuo-ku, Kobe 650-0047, Japan. nishiwak@cdb.riken.go.jp
Kubota
Yukihiko
Y
Chigira
Yuko
Y
Roy
Samir Kumar
SK
Suzuki
Maho
M
Schvarzstein
Mara
M
Jigami
Yoshifumi
Y
Hisamoto
Naoki
N
Matsumoto
Kunihiro
K
D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence / Q000235:genetics; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002465:Cell Movement / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D019483:Disintegrins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006031:Glycosylation; D006066:Gonads / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D032342:Muscle Cells / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D038081:Organogenesis / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D019483:Disintegrins; D006023:Glycoproteins; C410348:MIG-17 protein, C elegans; D008666:Metalloendopeptidases; D017766:Acid Anhydride Hydrolases; C481223:MIG-23 protein, C elegans; C023837:nucleoside-diphosphatase