title: [Study on exons 3 and 4 of alpha-synuclein gene in Chinese familial Parkinson disease patients].
abstract: OBJECTIVE
  To study the relationship of Chinese familial Parkinson disease with alpha-synuclein gene.
  
  
  METHODS
  Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis(PCR-HA) were employed to detect the abnormal mobilization in the familial Parkinson disease and sporadic Parkinson disease patients, then it was verified by gene sequencing.
  
  
  RESULTS
  No mutation was found in alpha-synuclein gene exons 3 and 4 by PCR-SSCP together with PCR-HA. An inserted c and an inserted t were found in intron 4, position 23 and position 67 respectively.
  
  
  CONCLUSIONS
  (1) Exons 3 and 4 of alpha-synuclein gene are not the mutational hot spots of Chinese familial Parkinson disease. (2) Two polymorphisms were found in intron 4 of alpha-synuclein gene. They are 23 ins c and 67 ins t.
authors.affiliation: Department of Neurology, the First Affiliated Hospital, San Yat-sen University, Guangzhu, Guangdong, 510080 PR China. chenxi73@163.com
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Yue-ting
authors.initials: YT
authors.lastname: Liu
authors.forename: Jin-ru
authors.initials: JR
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Xu
authors.forename: Yong-hui
authors.initials: YH
authors.lastname: Pan
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Meng
authors.forename: Shi-hui
authors.initials: SH
authors.lastname: Xing
mesh_terms: D000328:Adult; D000368:Aged; D005091:Exons; D005260:Female; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010300:Parkinson Disease / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D051843:Synucleins; D051844:alpha-Synuclein
chemical_list: D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein