Growth restoration in azuki bean and maize seedlings by removal of hypergravity stimuli.
Hypergravity stimuli, gravitational acceleration of more than 1 x g, decrease the growth rate of azuki bean epicotyls and maize coleoptiles and mesocotyls by decreasing the cell wall extensibility via an increase in the molecular mass of matrix polysaccharides. An increase in the pH in the apoplastic fluid is hypothesized to be involved in the processes of the increase in the molecular mass of matrix polysaccharides due to hypergravity. However, whether such physiological changes by hypergravity are induced by normal physiological responses or caused by physiological damages have not been elucidated. In the present study, we examined the effects of the removal of hypergravity stimuli on growth and the cell wall properties of azuki bean and maize seedlings to clarify whether the effects of hypergravity stimuli on growth and the cell wall properties are reversible or irreversible. When the seedlings grown under hypergravity conditions at 300 x g for several hours were transferred to 1 x g conditions, the growth rate of azuki bean epicotyls and maize coleoptiles and mesocotyls greatly increased within a few hours. The recovery of growth rate of these organs was accompanied by an immediate increase in the cell wall extensibility, a decrease in the molecular mass of matrix polysaccharides, and an increase in matrix polysaccharide-degrading activities. The apoplastic pH also decreased promptly upon the removal of hypergravity stimuli. These results suggest that plants regulate the growth rate of shoots reversibly in response to hypergravity stimuli by changing the cell wall properties, by which they adapt themselves to different gravity conditions. This study also revealed that changes in growth and the cell wall properties under hypergravity conditions could be recognized as normal physiological responses of plants. In addition, the results suggest that the effects of microgravity on plant growth and cell wall properties should be reversible and could disappear promptly when plants are transferred from microgravity to 1 x g. Therefore, plant materials should be fixed or frozen on orbit for detecting microgravity-induced changes in physiological parameters after recovering the materials to earth in space experiments.
Soga
K
K
Department of Biological Sciences, Osaka City University, Sumiyoshi-ku, Osaka, Japan. soga@sci.osaka-cu.ac.jp
Wakabayashi
K
K
Kamisaka
S
S
Hoson
T
T
D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D018548:Cotyledon / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D044644:Endo-1,3(4)-beta-Glucanase / Q000378:metabolism; D007887:Fabaceae / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018471:Hypergravity; D018546:Hypocotyl / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism
D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; C007916:hemicellulose; D044644:Endo-1,3(4)-beta-Glucanase