A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im.
Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-dependent manner. Elevated levels of CFI(m) suppressed cleavage at the primary poly(A) site of the pre-mRNA encoding the 68 kDa subunit of CFI(m). CFI(m)-mediated suppression of poly(A) site cleavage was dependent upon the presence of three copies of an RNA element initially identified by CFI(m)-SELEX. These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor.
Brown
Kirk M
KM
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA.
Gilmartin
Gregory M
GM
D020413:3' Untranslated Regions / Q000378:metabolism*; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D026723:Polyadenylation; D021122:Protein Subunits / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism*
D020413:3' Untranslated Regions; D021122:Protein Subunits; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C450562:cleavage factor Im, human; D039221:mRNA Cleavage and Polyadenylation Factors