title: A rapid and specific whole blood HPA-1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb-IIIa complexes.
abstract: The human platelet antigen 1 (HPA-1) system has been implicated in rare but severe diseases, such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and immune platelet refractoriness. We developed a flow cytometry assay for HPA-1 phenotyping using two commercial monoclonal antibodies, P2 and SZ21, directed against glycoprotein (GP) IIb-IIIa and GP IIIa respectively. One hundred and twenty-seven healthy controls were studied and ratios of mean fluorescence intensity for P2 and SZ21 discriminated between HPA-1a homozygotes and heterozygotes. These two monoclonal antibodies, coupled with flow cytometry represent a rapid and reliable tool for platelet HPA-1 typing to aid diagnosis.
authors.affiliation: Laboratoire d'hématologie et des maladies du sang, CHU de Poitiers, hôpital La Miletrie, Poitiers 86021, France. n.sorel@chu-poitiers.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Sorel
authors.forename: Severine
authors.initials: S
authors.lastname: Brabant
authors.forename: Luc
authors.initials: L
authors.lastname: Christiaens
authors.forename: Andre
authors.initials: A
authors.lastname: Brizard
authors.forename: Gerard
authors.initials: G
authors.lastname: Mauco
authors.forename: Laurent
authors.initials: L
authors.lastname: Macchi
mesh_terms: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D039661:Integrin beta3 / Q000276:immunology*; D010641:Phenotype; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C083451:human platelet antigen 1b