[The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology].
OBJECTIVE
To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.


METHODS
The expression of Bcl-2 protein in the early stage of acute myocardial ischemia/reperfusion(I/R) in rats were studyed with immunohistochemistry.


RESULTS
There was no expression of Bcl-2 protein in control group. The expression of Bcl-2 protein began to increase in myocytes 0.5 h after I/R in the ischemic areas and the expression of Bcl-2 protein in inner layer was stronger than in outer layer. The expression of Bcl-2 protein appears firstly in the inner layer of myocardium and extended gradually toward the outer layer 1 h after I/R. It was found that the expression of Bcl-2 protein in outer layer was stronger than in inner layer, then the expression of Bcl-2 protein became weaken gradually.


CONCLUSIONS
Bcl-2 gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.
Wang
Feng
F
Department of Forensic Pathology, Wannan Medical College, Wuhu 241001, China.
Hu
Yong-liang
YL
Li
Yong-hong
YH
D000818:Animals; D016757:Death, Sudden, Cardiac / Q000209:etiology; D005260:Female; D005554:Forensic Medicine; D007150:Immunohistochemistry; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D019253:Proto-Oncogene Proteins c-bcl-2