[Manifestation of glutathione S-transferase GSTM1 and GSTT1 in female patients with bleomycin-positive chromosome instability].
OBJECTIVE
The purpose of this research is to assess the incidence of gene polymorphisms coding the GSTM1 and GSTT1 enzymes in a population of female patients with chromosome instability.


METHODS
The PCR method was used to determine the genotype for GSTM1 and GSTT1. The breaks per cell and the percentage of damaged cells were calculated. The separation point used to diagnose chromosome instability in tested females was assumed to be 2.5.


RESULTS
In a tested population of 85 females with chromosome instability, the deletion of both the alleles of the GSTT1 gene was observed in 22 females (25% of the group) and deletion of both the alleles of the GSTM1 gene was observed in 42 females (53% of the group). In addition, the incidence of individual genes was calculated for the tested population. The X2 test showed that the differences between the observed and expected values of the tested genes were statistically immaterial, i.e. the likelihood of randomness for these differences exceeded 99%.


CONCLUSIONS
No relationship between the manifestation of genotypes for the GSTM1 and GSTT1 glutathione S-transferases and an increased chromosome instability confirmed with the bleomycin test was proven for a population of females with a neoplastic risk.
Waksmański
Bogdan
B
Katedry i Kliniki Perinatologii i Ginekologii SlAM w Katowicach.
Kamiński
Kazimierz
K
D000328:Adult; D001761:Bleomycin; D043171:Chromosomal Instability / Q000235:genetics; D025063:Chromosome Disorders / Q000201:enzymology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
D001761:Bleomycin; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase