title: Requirement for fibroblast growth factor 10 or fibroblast growth factor receptor 2-IIIb signaling for cecal development in mouse.
abstract: Epithelial-mesenchymal interactions are critical for the formation of gastrointestinal buds such as the cecum from the midgut, but the mechanisms regulating this process remain unclear. To investigate this problem, we have studied the temporal and spatial expression of key genes known to orchestrate branching morphogenesis. At E10.5, Fibroblast growth factor 10 (Fgf10) is specifically expressed in the mesenchyme above the future cecal epithelial bud, whereas Fgfr2b is found throughout the gut epithelium. From E11.5 onwards, Fgf10 expression is found throughout the cecum mesenchyme. Other relevant signaling molecules such as Sonic hedgehog, Wnt2b, and Tbx4 transcripts are found throughout the gut epithelium, including the cecum. Epithelial expression is also seen for Sprouty2, but only from E14.5 onwards. By contrast, Bone morphogenetic 4 (Bmp4) and Pitx2 are specifically expressed in the mesenchyme of the cecal bud at E11.5. Abrogation of either Fgf10 or Fgfr2b leads to similar phenotypes characterized by an arrest of epithelial invasion into the cecal mesenchymal tissue. However, a bud of undifferentiated cecal mesenchymal tissue is maintained throughout development. Our results further indicate that mesenchymal FGF10 acts mostly through the epithelial FGFR2b receptor; thereby triggering invasion of the midgut epithelium into the adjacent mesenchyme via an increased rate of epithelial proliferation at the tip of the cecum. Thus, FGF10 signaling via FGFR2b appears to be critical in the extension of the epithelium into the mesenchyme during cecal development.
authors.affiliation: Division of Developmental Biology, Department of Surgery, USC Keck School of Medicine and the Saban Research Institute of Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Burns
authors.forename: T J
authors.initials: TJ
authors.lastname: Fairbanks
authors.forename: F
authors.initials: F
authors.lastname: Sala
authors.forename: S
authors.initials: S
authors.lastname: De Langhe
authors.forename: A
authors.initials: A
authors.lastname: Mailleux
authors.forename: J P
authors.initials: JP
authors.lastname: Thiery
authors.forename: C
authors.initials: C
authors.lastname: Dickson
authors.forename: N
authors.initials: N
authors.lastname: Itoh
authors.forename: D
authors.initials: D
authors.lastname: Warburton
authors.forename: K D
authors.initials: KD
authors.lastname: Anderson
authors.forename: S
authors.initials: S
authors.lastname: Bellusci
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002432:Cecum / Q000033:anatomy & histology / Q000196:embryology*; D016923:Cell Death / Q000502:physiology; D004848:Epithelium / Q000196:embryology; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008648:Mesoderm / Q000502:physiology; D051379:Mice / Q000196:embryology* / Q000502:physiology; D008817:Mice, Mutant Strains; D009024:Morphogenesis; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D000199:Actins; C496464:Fgf10 protein, mouse; D051526:Fibroblast Growth Factor 10; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor