title: Intracellular trafficking during liver regeneration. Alterations in late endocytic and transcytotic pathways.
abstract: OBJECTIVE
  Liver growth, induced by partial hepatectomy of the organ is a precisely regulated process during which a radical reorganisation of metabolism occurs as the hepatocytes become committed to enter the cell cycle. Recent studies have shown the importance of the endocytic compartment in the control of lipid and protein intracellular trafficking but also in the control of the signal transduction events, which eventually will trigger the initiation of DNA synthesis and the subsequent cell division.
  
  
  METHODS
  We isolated endosomes at different times after partial hepatectomy in male rats and compared with endosomes isolated from sham-operated animals. Also, bile was collected and analysed by 2D-gel electrophoresis.
  
  
  RESULTS
  The amount of late endosomes isolated from regenerating livers decreased, concomitant with decreased cathepsin D specific enzyme activity. Furthermore, secretion of horseradish peroxidase, pIgA and transferrin increased in the pre-replicative phase of liver regeneration.
  
  
  CONCLUSIONS
  At the early stages of liver regeneration, the hepatocellular transport pathway towards degradation (late endosomes and lysosomal pathway) decreases, but the transcytosis and the bile secretion of several major proteins increases.
authors.affiliation: Departament de Biologia Cellular, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036 Barcelona, Spain.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Fernández
authors.forename: Silvia
authors.initials: S
authors.lastname: Turró
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ingelmo-Torres
authors.forename: Carlos
authors.initials: C
authors.lastname: Enrich
authors.forename: Albert
authors.initials: A
authors.lastname: Pol
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D001692:Biological Transport; D001769:Blood / Q000378:metabolism*; D002402:Cathepsin D / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D006498:Hepatectomy / Q000379:methods; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D007425:Intracellular Membranes / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011184:Postoperative Period; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D006735:Horseradish Peroxidase; D002402:Cathepsin D