A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces.
A new design for a high pressure MALDI-FTMS instrument is described and initial data are shown. The instrument incorporates a large, 10 cm x 10 cm, sample translation stage to accommodate and position the MALDI target. The new instrument allows coupling to a wide variety of surface techniques such as gel electrophoresis or surface plasmon resonance. Coupling to thin layer chromatography is shown. Furthermore, a new nozzle design allows high pressure collisional cooling sufficient to stabilize gangliosides while minimizing the gas load on the system.
O'Connor
Peter B
PB
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA. poconnor@bu.edu
Budnik
Bogdan A
BA
Ivleva
Vera B
VB
Kaur
Parminder
P
Moyer
Susanne C
SC
Pittman
Jason L
JL
Costello
Catherine E
CE
D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*
D005732:Gangliosides