Intestinal intussusception associated with adenovirus infection in Mexican children.
Formalin-fixed intestinal tissue specimens from 12 Mexican pediatric patients with intussusception were examined for the presence of adenovirus. Four patients (33%) had detectable adenovirus antigen in epithelial cells as determined by using immunohistochemical analysis. Two of the patients with positive immunohistochemical results had antigens in dendritic and mononuclear inflammatory cells, and 3 patients had positive results for species C adenovirus by in situ hybridization using adenovirus species-specific probes (A-F). A real-time polymerase chain reaction assay specific for species C (nonenteric) adenoviruses was used to confirm immunohistochemical results and to amplify adenovirus DNA for sequencing. A sequence similar to that for adenovirus serotype 1 was found in 1 patient, serotype 2 in another, and serotype 6 in a third; in the fourth patient, the sequence was indeterminate between serotypes 2 and 6. The assays used in this study proved useful for the identification of species C adenoviruses in formalin-fixed specimens from Mexican pediatric patients with intussusception.
Guarner
Jeannette
J
Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G32, Atlanta, GA 30333, USA.
de Leon-Bojorge
Beatriz
B
Lopez-Corella
Eduardo
E
Ferebee-Harris
Tara
T
Gooding
Linda
L
Garnett
C T
CT
Shieh
Wun-Ju
WJ
Dawson
Jacqueline
J
Erdman
Dean
D
Zaki
Sherif R
SR
D000257:Adenoviridae Infections / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D007443:Intussusception / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D000956:Antigens, Viral; D004279:DNA, Viral