title: Effect of heat-treated amphotericin B on renal and fungal cytotoxicity.
abstract: The purpose of this investigation was to determine the cytotoxicity of amphotericin B (AMB; trade name Fungizone [FZ]) following the administration of FZ and a heat-treated form of FZ (HFZ) to LLC-PK(1) pig kidney cells and Cryptococcus neoformans var. gattii cells. HFZ was significantly less toxic to kidney cells than FZ at all concentrations tested. For both FZ and HFZ, the concentration range which resulted in a 50% reduction of the growth of fungal cells was 0.125 to 1 mg/ml. These findings suggest that heat treatment decreases AMB's renal cytotoxicity without modifying its antifungal activity.
authors.affiliation: School of Occupational and Environmental Hygiene, Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
authors.forename: Karen
authors.initials: K
authors.lastname: Bartlett
authors.forename: Edwin
authors.initials: E
authors.lastname: Yau
authors.forename: Scott C
authors.initials: SC
authors.lastname: Hartsel
authors.forename: Alison
authors.initials: A
authors.lastname: Hamer
authors.forename: Gina
authors.initials: G
authors.lastname: Tsai
authors.forename: Dan
authors.initials: D
authors.lastname: Bizzotto
authors.forename: Kishor M
authors.initials: KM
authors.lastname: Wasan
mesh_terms: D000666:Amphotericin B / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects; D005658:Fungi / Q000187:drug effects*; D006358:Hot Temperature; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D018374:LLC-PK1 Cells; D008826:Microbial Sensitivity Tests; D013552:Swine
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B