Neutral, acidic, and basic derivatives of anthranilamide that confer different formal charge to reducing oligosaccharides.
To facilitate the use of oligosaccharides as analytical tools in biological studies, we have designed, synthesized, and conjugated to maltosaccharides a novel series of homologous small fluorescent moieties that differ in formal charge. These moieties are amide derivatives of anthranilic acid: uncharged N-(2-aminobenzoyl)glycinamide (ABGlyAmide; 2), acidic N,N-dimethyl-N(')-(2-aminobenzoyl)ethylenediamine (ABGlyDIMED; 3), and basic N-(2-aminobenzoyl)glycine (ABGly; 1). Routes for synthesis and optimal reaction conditions for glycoconjugation by conventional reductive amination are presented, as is the compatibility of these adducts with common analytical and preparative chromatographic methods, including RP-HPLC and HPAEC-PAD. These novel anthranilic acid derivatives confer both fluorescence and defined charge to oligosaccharides, and so enhance the repertoire of chromatographic and analytical methods for which anthranilic acid can be used. Furthermore, because glucosaccharides have rigid solution structure, these small fluorescent adducts with different formal charge are ideal tools for molecular sizing studies of membrane pores.
Locke
Darren
D
Department of Pharmacology and Physiology, New Jersey Medical School, UMDNJ, 185 South Orange Avenue, Newark, NJ 07103, USA. lockeda@umdnj.edu
Bevans
Carville G
CG
Wang
Lai Xi
LX
Zhang
Ye
Y
Harris
Andrew L
AL
Lee
Yuan C
YC
D000143:Acids / Q000737:chemistry; D000327:Adsorption; D000588:Amines / Q000737:chemistry; D002236:Carbohydrate Conformation; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012996:Solutions / Q000737:chemistry; D013057:Spectrum Analysis; D055672:Static Electricity; D062367:ortho-Aminobenzoates / Q000138:chemical synthesis / Q000737:chemistry*
D000143:Acids; D000588:Amines; D007477:Ions; D009844:Oligosaccharides; D012996:Solutions; D062367:ortho-Aminobenzoates; C000219:anthranilamide