Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome.
The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped.
Liu
Dong P
DP
Jackson Laboratory, Bar Harbor, ME, USA.
Schmidt
Cecilia
C
Billings
Timothy
T
Davisson
Muriel T
MT
D000465:Algorithms; D000818:Animals; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000175:diagnosis* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D005820:Genetic Testing / Q000379:methods*; D005838:Genotype; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity