title: Development of a microtiter plate ELISA and a dipstick ELISA for the determination of the organophosphorus insecticide fenthion.
abstract: In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA showed an IC(50) value of 1.2 microg/L with a detection limit of 0.1 microg/L. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides. The use of the dipstick format using Immunodyne as a support membrane allowed the quick visual detection of fenthion in concentrations >10 microg/L. The IC(50) value of the dipstick format using reflectance detection was 15 microg/L with a detection limit of 0.5 microg/L. The recoveries of fenthion from spiked vegetable samples using the two formats without any prior enrichment or cleanup steps were 87-116%.
authors.affiliation: Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Young Ae
authors.initials: YA
authors.lastname: Cho
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Lee
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005284:Fenthion / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D011786:Quality Control; D011934:Reagent Strips
chemical_list: D007306:Insecticides; D011934:Reagent Strips; D005284:Fenthion