title: Comparison of Th1/Th2 cytokine profiles of initial wound healing of rats induced by PDCM and e-PTFE.
abstract: Because periodontal regeneration is vital in the modern treatment of periodontal defects, artificial membranes have become a key component for predictable outcomes. Herein a new ranking system to analyze the expression of different cytokines around regenerative membranes is introduced. Porcine dermal collagen membranes (PDCMs) and GORE-TEX (e-PTFE) membranes were used for implantation in a Sprague Dawley rat model. Tissue samples were harvested at three time intervals (7, 10, and 14 days); then an immunohistochemical ranking process was conducted to determine the intensity of the selected Th1/Th2 cytokines [Th1: interleukin-2 (IL-2) and interferone-gamma (IFN-gamma); Th2: IL-4, IL-10, and IL-13]. The results show that the intensities of IL-2 cytokine in PDCM groups were slightly higher than those of e-PTFE groups but without statistical significance. The level of interferon-gamma in PDCM groups was lower than that of e-PTFE groups, but also without significant differences. However, expressions of Th2 cytokines (IL-4, IL-10, and IL-13) induced by e-PTFE were generally higher than those of PDCM and control groups at all times (Mann-Whitney U test, p < 0.05). In a comparison of the mean ratio of IL-2/IL-4 with the use of the Mann-Whitney U test, data for the PDCM group were generally higher than those of the e-PTFE group, with statistical significance at all time intervals (p < 0.05). A descending order of the intensity ratio of IL-2/IL-4 was PDCM groups > control groups > e-PTFE groups. These findings indicate that the cytokine profiles of PDCM, in connection with the GTR technique, demonstrate a higher trend toward Th1-dominated responses and may protect against periodontal tissue destruction, as compared to the Th2-dominated responses of e-PTFE.
authors.affiliation: Department of Periodontics, Graduate Institute of Oral Rehabilitation, College of Oral Medicine, Taipei Medical University, Taiwan. jackson@tmu.edu.tw
authors.forename: Hsein-Kun
authors.initials: HK
authors.lastname: Lu
authors.forename: Meng-Tsung
authors.initials: MT
authors.lastname: Ko
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Wu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology; D016207:Cytokines / Q000187:drug effects*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000187:drug effects; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D018417:Th1 Cells / Q000187:drug effects*; D018418:Th2 Cells / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma; D011138:Polytetrafluoroethylene; D003094:Collagen