Distribution and expression of non-muscle myosin light chain kinase in rabbit livers.
OBJECTIVE
To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.


METHODS
Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.


RESULTS
The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.


CONCLUSIONS
nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.
Zhu
Hua-Qing
HQ
Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China.
Wang
Yuan
Y
Hu
Ruo-Lei
RL
Ren
Bin
B
Zhou
Qing
Q
Jiang
Zhi-Kui
ZK
Gui
Shu-Yu
SY
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011817:Rabbits
D009219:Myosin-Light-Chain Kinase; C482520:non-muscle myosin light chain kinase, rabbit