[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx].
In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.
Zhang
Ling
L
Cancer Research Institute, Xiangya Medical School of Central South University, Changsha 410078, China.
Lan
Ke
K
Feng
Xiang-Ling
XL
Qiao
Gui-Lin
GL
Shen
Xin-Ming
XM
Shi
Yi-Min
YM
Li
Hong
H
Yao
Kai-Tai
KT
D000818:Animals; D002460:Cell Line; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009305:Nasopharynx / Q000737:chemistry / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013270:Stomach / Q000737:chemistry; D014059:Tongue / Q000737:chemistry / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins