Arrays of immobilized oligonucleotides--contributions to nucleic acids technology.
The interactions of nucleic acids technology and the technology of arrayed nucleic acids are described, showing the interdependence of nucleic acids chemistry, surface chemistry, (micro-) technology and the requirements of bio-medical applications. The methods and problems of the production of large numbers of oligonucleotides as well as the methods of arraying oligonucleotides are highlighted. The basic approaches, in-situ synthesis and postsynthetic immobilization, are described with a special emphasis on the postsynthetic immobilization of ready-made oligonucleotides on support materials. Techniques for the detection of nucleic acids interactions on arrays are outlined in brief.
Seliger
H
H
University of Ulm, Sektion Chemische Funktionen in Biosystemen, Ulm, Germany. Hartmut.Seliger@physik.uni-ulm.de
Hinz
M
M
Happ
E
E
D000327:Adsorption; D001709:Biotechnology / Q000379:methods; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D004867:Equipment Design; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties
D015342:DNA Probes; D009841:Oligonucleotides