Continuous measurement of glutamate and hydrogen peroxide using a microfabricated biosensor for studying the neurotoxicity of tributyltin.
We first measured the effects of trace levels of an endocrine disruptor, tributyltin (TBT), on the secretion response from nerve cells using a microfabricated biosensor designed for the continuous measurement of L-glutamate and hydrogen peroxide. We observed higher and long-lasting glutamate and hydrogen peroxide concentrations from the cells when cultured rat cortical neurons were exposed to TBT. Glutamate and hydrogen peroxide release was induced even when we reduced the TBT concentration to 10 nM. This concentration is about two orders of magnitude lower than the concentration that induced apoptosis-like cell death. We also report on the effects of NMDA and non-NMDA receptor antagonists, which can help us to understand the mechanism of TBT neurotoxicity.
Kurita
Ryoji
R
NTT Advanced Technology, 3-1 Morinosato-Wakamiva, Atsugi, Kanagawa 243-0124, Japan.
Hayashi
Katsuyoshi
K
Torimitsu
Keiichi
K
Niwa
Osamu
O
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004566:Electrodes; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000032:analysis* / Q000378:metabolism; D015386:Hazardous Substances / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D044085:Microfluidics / Q000295:instrumentation; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D014220:Trialkyltin Compounds / Q000633:toxicity*
D018691:Excitatory Amino Acid Antagonists; D015386:Hazardous Substances; D014220:Trialkyltin Compounds; D018698:Glutamic Acid; C011559:tributyltin; D006861:Hydrogen Peroxide