title: Multiple EphB receptor tyrosine kinases shape dendritic spines in the hippocampus.
abstract: Here, using a genetic approach, we dissect the roles of EphB receptor tyrosine kinases in dendritic spine development. Analysis of EphB1, EphB2, and EphB3 double and triple mutant mice lacking these receptors in different combinations indicates that all three, although to varying degrees, are involved in dendritic spine morphogenesis and synapse formation in the hippocampus. Hippocampal neurons lacking EphB expression fail to form dendritic spines in vitro and they develop abnormal spines in vivo. Defective spine formation in the mutants is associated with a drastic reduction in excitatory glutamatergic synapses and the clustering of NMDA and AMPA receptors. We show further that a kinase-defective, truncating mutation in EphB2 also results in abnormal spine development and that ephrin-B2-mediated activation of the EphB receptors accelerates dendritic spine development. These results indicate EphB receptor cell autonomous forward signaling is responsible for dendritic spine formation and synaptic maturation in hippocampal neurons.
authors.affiliation: Division of Biomedical Sciences, University of California Riverside, Riverside, CA 92521, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Henkemeyer
authors.forename: Olga S
authors.initials: OS
authors.lastname: Itkis
authors.forename: Michelle
authors.initials: M
authors.lastname: Ngo
authors.forename: Peter W
authors.initials: PW
authors.lastname: Hickmott
authors.forename: Iryna M
authors.initials: IM
authors.lastname: Ethell
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003712:Dendrites / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D015536:Down-Regulation / Q000235:genetics; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D051379:Mice; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009154:Mutation / Q000235:genetics; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D011940:Receptor Aggregation / Q000235:genetics; D036225:Receptor, EphB1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D036183:Receptor, EphB2 / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D036223:Receptor, EphB3 / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D036081:Receptors, Eph Family / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000201:enzymology* / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D005680:gamma-Aminobutyric Acid; D036225:Receptor, EphB1; D036183:Receptor, EphB2; D036223:Receptor, EphB3; D036081:Receptors, Eph Family