NapGH components of the periplasmic nitrate reductase of Escherichia coli K-12: location, topology and physiological roles in quinol oxidation and redox balancing.
Nap (periplasmic nitrate reductase) operons of many bacteria include four common, essential components, napD, napA, napB and napC (or a homologue of napC ). In Escherichia coli there are three additional genes, napF, napG and napH, none of which are essential for Nap activity. We now show that deletion of either napG or napH almost abolished Nap-dependent nitrate reduction by strains defective in naphthoquinone synthesis. The residual rate of nitrate reduction (approx. 1% of that of napG+ H+ strains) is sufficient to replace fumarate reduction in a redox-balancing role during growth by glucose fermentation. Western blotting combined with beta-galactosidase and alkaline phosphatase fusion experiments established that NapH is an integral membrane protein with four transmembrane helices. Both the N- and C-termini as well as the two non-haem iron-sulphur centres are located in the cytoplasm. An N-terminal twin arginine motif was shown to be essential for NapG function, consistent with the expectation that NapG is secreted into the periplasm by the twin arginine translocation pathway. A bacterial two-hybrid system was used to show that NapH interacts, presumably on the cytoplasmic side of, or within, the membrane, with NapC. As expected for a periplasmic protein, no NapG interactions with NapC or NapH were detected in the cytoplasm. An in vitro quinol dehydrogenase assay was developed to show that both NapG and NapH are essential for rapid electron transfer from menadiol to the terminal NapAB complex. These new in vivo and in vitro results establish that NapG and NapH form a quinol dehydrogenase that couples electron transfer from the high midpoint redox potential ubiquinone-ubiquinol couple via NapC and NapB to NapA.
Brondijk
T Harma C
TH
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
Nilavongse
Arjaree
A
Filenko
Nina
N
Richardson
David J
DJ
Cole
Jeffrey A
JA
D001120:Arginine / Q000737:chemistry; D001483:Base Sequence; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D009573:Nitrites / Q000378:metabolism; D009876:Operon; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D033903:Periplasmic Proteins / Q000032:analysis / Q000502:physiology*; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013347:Subcellular Fractions / Q000201:enzymology; D020798:Two-Hybrid System Techniques; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism*
D029968:Escherichia coli Proteins; D009573:Nitrites; D033903:Periplasmic Proteins; D011993:Recombinant Fusion Proteins; D014451:Ubiquinone; D001120:Arginine; D010088:Oxidoreductases; C487715:NapG protein, E coli; C487716:NapH protein, E coli; D009565:Nitrate Reductases; C487717:NapC protein, E coli; D050901:Nitrate Reductase; C494404:napA protein, E coli; C003741:ubiquinol