title: Species-specific transformation of T cells by HV(MNE).
abstract: HV(MNE) is an Epstein-Barr virus (EBV)-like lymphocryptovirus (LCV) originally isolated from a Macaca nemestrina with CD8(+) T cell mycosis fungoides/cutaneous T cell lymphoma (Blood 98 (2001), 2193). HV(MNE) transforms rabbit T cells in vitro and causes T cell lymphoma in New Zealand white rabbits. Here we demonstrate that HV(MNE) also immortalizes T cells from mustached tamarins but not those from owl monkeys, common marmosets, squirrel monkeys, black-capped capuchins, and humans. Cytogenetic and FACS analysis revealed the true origin and T cell lineage of the transformed tamarin T cell lines. Tamarin T cells contained HV(MNE) DNA sequence and displayed a decreased requirement for the IL-2 cytokine for growth. Thus, this EBV-like virus from M. nemestrina differs from the other EBV-like viruses found in nonhuman primates inasmuch as it appears to preferentially transform T cells.
authors.affiliation: Animal Models and Retroviral Vaccines Section, National Cancer Institute, 41/D804, Bethesda, MD 20892-5055, USA.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Ferrari
authors.forename: Liljana
authors.initials: L
authors.lastname: Stevceva
authors.forename: Phillip
authors.initials: P
authors.lastname: Markham
authors.forename: Genoveffa
authors.initials: G
authors.lastname: Franchini
mesh_terms: D000818:Animals; D001029:Aotus trivirgatus; D002144:Callithrix; D002427:Cebidae / Q000145:classification; D002428:Cebus; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D018143:Lymphocryptovirus / Q000472:pathogenicity* / Q000502:physiology; D008213:Lymphocyte Activation; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D012452:Saguinus; D012453:Saimiri; D013045:Species Specificity; D013601:T-Lymphocytes / Q000502:physiology / Q000821:virology*
chemical_list: D004279:DNA, Viral