[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].
The cDNA of human B lymphocyte stimulator C-terminal peptide (C-BLyS) was amplified by nested PCR from cDNA library of human fetal brain. The expression plasmid pT7450-C-BLyS was constructed and transformed into E. coli BL21 CodonPlus (DE3) RIL which can recognize many rare codons. The C-BLyS protein was expressed as inclusion body in E. coli BL21 CodonPlus (DE3) RIL and the inclusion body of C-BLyS was denatured and then refolded by dialysis and purified by ion exchange chromatography. The refolded and purified C-BLyS can specifically bind with BCMA-Fc, a fusion protein of BLyS receptor and human IgG1 Fc. Furthermore, C-BLyS is proved to be an effective stimulator in mouse splenocytes proliferation in vitro and effective immunostimulant in vivo.
Tian
Rui-Yang
RY
Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China.
Han
Wei
W
Yu
Ying
Y
Chen
Yue
Y
Yu
Gu-Song
GS
Yang
Sheng-Li
SL
Gong
Yi
Y
D000818:Animals; D053264:B-Cell Activating Factor; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D017510:Protein Folding; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
D053264:B-Cell Activating Factor; C120197:BLyS receptor; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008565:Membrane Proteins; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C505719:TNFSF13B protein, human; C505720:Tnfsf13b protein, mouse; D014409:Tumor Necrosis Factor-alpha; D009113:Muramidase