[Different effects of several signal pathway after liver transplantation].
OBJECTIVE
To explorer the change of several signal pathway and their signal after liver transplantation.


METHODS
Classified 34 punctured donor liver samples and 10 normal liver samples as A (no rejection) groups, B (mild/moderate acute rejection) groups, C (serious acute rejection) groups, D (chronic rejection/fibrosis) groups and E (control) groups, MAPK, Ras and p53 were performed immunohistochemistry analysis and image analysis. MAPK and Ras were performed in situ hybridizition. Then image analysis was performed.


RESULTS
The protein expression of MAPK, Ras, increase by turns of A, B and C groups (1.42+/-0.28, 3.88+/-0.87, 6.68+/-0.57 in MAPK; 1.27+/-0.12, 2.80+/-0.30, 3.93+/-0.20 in Ras; corresponding), and decrease by turns of D and E groups (1.49+/-0.37, 0.88+/-0.20 in MAPK; 1.47+/-0.21, 1.01+/-0.12 in Ras; corresponding, F=178.39 in MAPK and 320.59 in Ras, groups B, C vs groups A, D, E, P<0.001 in MAPK and Ras), The protein expression of p53 is higher in treated groups (The results of groups A to E are 2.09+/-0.13, 2.39+/-0.11, 2.03+/-0.19, 2.26+/-0.18 and 0.35+/-0.08, corresponding, F=360.08, groups E vs groups A, B, C, D, P<0.001). Expression of MAPK, Ras mRNA is as same as that of protein.


CONCLUSIONS
The MAPKs pathway has role in rejection response after liver transplantation. And it seemed that the MAPKs and p53 are one regulation mechanism for protecting the hepatocyte from damage after liver transplantation.
Li
Guo-xun
GX
Institut of Organ transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Gong
Nian-qiao
NQ
Ye
Qi-fa
QF
Guo
Hui
H
D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016031:Liver Transplantation; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis; D015398:Signal Transduction / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D018631:ras Proteins / Q000032:analysis
D016159:Tumor Suppressor Protein p53; D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins