title: [Amplification of brain-derived neurotrophic factor receptor trkB gene and construction of its eukaryotic expression vector].
abstract: OBJECTIVE
  To construct a recombinant eukaryotic expression vector of rat brain-derived neurotrophic factor receptor trkB gene.
  
  
  METHODS
  Using the total RNA extracted from rat brain tissue as the template, the trkB gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with a pair of specific primers containing the restriction sites of EcoRI and BamHI. The amplified fragment of trkB gene was digested with EcoRI and BamHI, and then subcloned into cloning vector pMD18-T and then expression vector pEGFP-C2. The recombinant plasmid was identified by restriction endonuclease analysis and PCR.
  
  
  RESULTS
  The amplified DNA fragment was about 1 461 bp in length, and enzyme digestion and PCR analysis showed that trkB gene had been successfully cloned into the vectors pMD18-T and pEGFP-C2.
  
  
  CONCLUSIONS
  The trkB gene of rat has been successfully amplified and cloned into the eukaryotic expression vector pEGFP-C2.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. arteries@263.net
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Qiu
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Qin
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D010957:Plasmids; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020813:Receptor, trkB