Analysis of gene expression profiles in largemouth bass exposed to 17-beta-estradiol and to anthropogenic contaminants that behave as estrogens.
Novel molecular based methods are being developed to study changes in gene expression in wildlife exposed to anthropogenic chemicals. Gene arrays, in particular, are useful tools that can be used to simultaneously monitor hundreds to thousands of genes within a single experiment, giving an investigator the ability to determine how exposure affects multiple metabolic pathways. These methods are thought to be both sensitive and able to reveal biochemical mechanisms of action. A largemouth bass (LMB) array containing 132 genes has been designed to study the impact of gene expression in male fish exposed to 17-beta estradiol or to the compounds 4-nonylphenol (4-NP) or 1,1-dichloro-2, 2-bis (p-chlorophenyl) ethylene (p,p'-DDE). The results of these experiments demonstrate distinct gene expression patterns in LMB exposed to these compounds.
Larkin
P
P
Department of Biochemistry and Molecular Biology, Center for Biotechnology, University of Florida, PO Box 100156 HC, 1600 Archer Road, MSB Building, Room MG-42, Gainesville, FL 32610, USA.
Sabo-Attwood
T
T
Kelso
J
J
Denslow
N D
ND
D000818:Animals; D000835:Animals, Wild; D001492:Bass / Q000235:genetics* / Q000502:physiology; D004781:Environmental Exposure; D004958:Estradiol / Q000633:toxicity*; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D014874:Water Pollutants, Chemical; D004958:Estradiol