title: [The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology].
abstract: OBJECTIVE
  To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.
  
  
  METHODS
  The expression of Bcl-2 protein in the early stage of acute myocardial ischemia/reperfusion(I/R) in rats were studyed with immunohistochemistry.
  
  
  RESULTS
  There was no expression of Bcl-2 protein in control group. The expression of Bcl-2 protein began to increase in myocytes 0.5 h after I/R in the ischemic areas and the expression of Bcl-2 protein in inner layer was stronger than in outer layer. The expression of Bcl-2 protein appears firstly in the inner layer of myocardium and extended gradually toward the outer layer 1 h after I/R. It was found that the expression of Bcl-2 protein in outer layer was stronger than in inner layer, then the expression of Bcl-2 protein became weaken gradually.
  
  
  CONCLUSIONS
  Bcl-2 gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.
authors.affiliation: Department of Forensic Pathology, Wannan Medical College, Wuhu 241001, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Yong-liang
authors.initials: YL
authors.lastname: Hu
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D000818:Animals; D016757:Death, Sudden, Cardiac / Q000209:etiology; D005260:Female; D005554:Forensic Medicine; D007150:Immunohistochemistry; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2