Effect of a nucleoside/nucleotide-free diet in rat allogenic small intestinal transplantation.
The aim of this study is to estimate the effect of nucleoside (NS) and nucleotide (NT) on the recipient and graft immune response after rat allogenic small intestinal transplantation. Seven-week-old Lewis rats were randomly assigned to two groups, including the NS/NT free group ( n=6) and the NS/NT supplemented group ( n=6), according to the diet received. The recipient Lewis rats were each given diet for 12 days, and then, on the nineteenth day of gestation, a 2 cm jejunum from the donor fetal Fischer rat was transplanted into the abdominal wall of the recipient rats using a non-vascular anastomotic technique. The recipient rats were killed on day 2 after transplantation, and then the recipient plasma interleukin-2 (IL-2) level was measured. In addition, the histological findings of the graft were analyzed. The IL-2 level of the NS/NT free group was significantly lower than that of the NS/NT supplemented group. In order to determine the grade of rejection, the morphological findings were blindly graded on a scale of 0-4. The mean grade of the NS/NT free group was also significantly lower than that of the NS/NT supplemented group. The NS/NT free diet is therefore considered to have an immunosuppressive effect on rat allogenic small intestinal transplantation based on the recipient plasma IL-2 levels and the histological findings of the grafts.
Ogita
Keiko
K
Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582, Fukuoka, Japan. ogita@pedsurg.med.kyushu-u.ac.jp
Suita
Sachiyo
S
Taguchi
Tomoaki
T
Nakamura
Masatoshi
M
Uesugi
Toru
T
D034861:Abdominal Wall / Q000601:surgery; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D006084:Graft Rejection / Q000473:pathology; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000097:blood; D007413:Intestinal Mucosa / Q000473:pathology; D007583:Jejunum / Q000276:immunology / Q000473:pathology / Q000637:transplantation*; D009705:Nucleosides / Q000008:administration & dosage*; D009711:Nucleotides / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000276:Adjuvants, Immunologic; D015415:Biomarkers; D007376:Interleukin-2; D009705:Nucleosides; D009711:Nucleotides