title: Evaluation of liquid chromatography column retentivity using macromolecular probes. IV. Poly(ethylene glycol) bonded phase.
abstract: Interaction properties of the novel HPLC silica gel-poly(ethylene glycol) (PEG) bonded phase were evaluated applying polymeric test substances, viz. polystyrenes, poly(methyl methacrylate)s, poly(ethylene oxide)s and poly(2-vinyl pyridine)s, and eluents of different polarities. Silanols on the silica gel surface are well shielded by the PEG phase, and silanophilic adsorption of macromolecules is suppressed in comparison with most silica C(18) bonded phases. The adsorption of solutes on the -OH groups of the PEG phase seems to be low as well. The partition of macromolecules in favor of the PEG phase is inferior to that observed in case of the silica C(18) phases. The volume of the PEG bonded phase is small and it is supposed that the PEG chains assume flat conformation on the silica gel surface.
authors.affiliation: Laboratory of Liquid Chromatography, Polymer Institute, Dubravska Cesta 9, Slovak Academy of Sciences, SK-842 36 Bratislava, Slovakia. dusan.berek@savba.sk
authors.forename: Dusan
authors.initials: D
authors.lastname: Berek
authors.forename: Raniero
authors.initials: R
authors.lastname: Mendichi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005663:Furans / Q000032:analysis; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000032:analysis; D011137:Polystyrenes / Q000737:chemistry; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005663:Furans; D011137:Polystyrenes; C018674:tetrahydrofuran; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D019904:Polymethyl Methacrylate