title: [Microcytomorphometric video-image detection of nuclear chromatin in ovarian cancer].
abstract: Technology of detection of tissue preparates precisious evaluates contents of nuclear chromatine, largeness and shape of cellular nucleus, indicators of mitosis, DNA index, ploidy, phase-S fraction and other parameters. Methods of detection of picture are: microcytomorphometry video-image (MCMM-VI), flow, double flow and activated by fluorescence. Diagnostic methods of malignant neoplasm of ovary are still nonspecific and not precise, that is a reason of unsatisfied results of treatment.
  
  
  OBJECTIVE
  Evaluation of microcytomorphometric measurements of nuclear chromatine histopathologic tissue preparates (HP) of ovarian cancer and comparison to normal ovarian tissue.
  
  
  METHODS
  Estimated 10 paraffin embedded tissue preparates of serous ovarian cancer, 4 preparates mucinous cancer and 2 cases of tumor Kruckenberg patients operated in Clinic of Perinatology and Gynaecology Silesian Medical Academy in Zabrze in period 2001-2002, MCMM-VI estimation based on computer aided analysis system: microscope Axioscop 20, camera tv JVCTK-C 1380, CarlZeiss KS Vision 400 rel.3.0 software. Following MCMM-VI parameters assessed: count of pathologic nucleus, diameter of nucleus, area, min/max diameter ratio, equivalent circle diameter (Dcircle), mean of brightness (mean D), integrated optical density (IOD = area x mean D), DNA index and 2.5 c exceeding rate percentage (2.5 c ER%). MCMM-VI performed on the 160 areas of 16 preparates of cancer and 100 areas of normal ovarian tissue. Statistical analysis was performed by used t-Student test.
  
  
  RESULTS
  We obtained stastistically significant higher values parameters of nuclear chromatine, DI, 2.5 c ER of mucinous cancer and tumor Kruckenberg comparison to serous cancer. MCMM-VI parameters of chromatine malignant ovarian neoplasm were statistically significantly higher than normal ovarian tissue.
  
  
  CONCLUSIONS
  Cytometric and karyometric parametres of nuclear chromatine estimated MCMM-VI are useful in the diagnostics and prognosis of ovarian cancer.
authors.affiliation: Katedry i Kliniki Perinatologii i Ginekologii w Zabrzu Sl. A.M.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Grzonka
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kamiński
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kaźmierczak
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry*; D002843:Chromatin / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted; D007620:Karyometry; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000648:ultrastructure*; D010053:Ovary / Q000737:chemistry / Q000648:ultrastructure; D013997:Time Factors; D014743:Videotape Recording
chemical_list: D002843:Chromatin; D004273:DNA, Neoplasm