Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications.
Magnetic hydrophilic non-porous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of several kinds of magnetite. It was found that some components used in the preparation of magnetic carriers interfered with polymerase chain reaction (PCR). Influence of non-magnetic and magnetic microspheres, including magnetite nanoparticles and various components used in their synthesis, on the PCR course was thus investigated. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interfering magnetic microspheres. The method enabled verification of the incorporation of magnetite nanoparticles in the particular methacrylate-based polymer microspheres and evaluation of suitability of their application in PCR. Preferably, electrostatically stabilized colloidal magnetite (ferrofluid) should be used in the design of new magnetic methacrylate-based microspheres by dispersion polymerization.
Spanová
Alena
A
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Horák
Daniel
D
Soudková
Eva
E
Rittich
Bohuslav
B
D001644:Bifidobacterium / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004852:Epoxy Compounds; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D004852:Epoxy Compounds; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate; C007870:glycidyl methacrylate