Morphological evidence of reinnervation of the baroreceptive regions in sinoaortic-denervated rats.
1. The arterial baroreflex (ABR) plays an important role in the maintenance of the stability of blood pressure. Sinoaortic denervation (SAD) destroys the integrity of the reflex arc and produces severe organ damage in rats. However, partial recovery of ABR function has been observed following chronic denervation. The aim of the present study was to determine whether there was morphological evidence of reinnervation of the aortic arch and carotid sinus following SAD. 2. A substantial body of physiological and morphological evidence suggests that substance P (SP) may be a neurotransmitter contained in first-order sensory baroreceptor afferents; therefore, the patterns of vascular SP and neurofilament (NF) immunoreactive (IR) innervation of the aortic arch and carotid sinus were investigated in the present study. 3. Ten-week-old male Sprague-Dawley rats underwent SAD or sham operation. Whole mounts of carotid bifurcation and aortic arch were prepared for immunohistochemical study at various time points (1, 9 and 16 weeks after operation). 4. The results of computerized image analysis show that the mean density of NF- and SP-IR nerves of SAD rats 9 and 16 weeks after operation increased gradually and significantly compared with that of rats 1 week after operation. 5. In conclusion, the results indicate that there is reinnervation of the aortic arch and carotid sinus by NF- and SP-IR fibres in SAD rats, which may be the morphological basis for the partial restoration of ABR function over time after SAD.
Cai
Guo-Jun
GJ
Department of Pharmacology, Second Military Medical University, Shanghai, China.
Li
Ling
L
Xie
He-Hui
HH
Xu
Jia-Jun
JJ
Miao
Chao-Yu
CY
Su
Ding-Feng
DF
D000818:Animals; D001013:Aorta, Thoracic / Q000294:innervation* / Q000601:surgery / Q000648:ultrastructure; D017704:Baroreflex / Q000502:physiology; D002346:Carotid Sinus / Q000294:innervation* / Q000601:surgery / Q000648:ultrastructure; D003714:Denervation / Q000379:methods; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D009475:Neurons, Afferent / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011311:Pressoreceptors / Q000033:anatomy & histology / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D016900:Neurofilament Proteins; D013373:Substance P