title: The effect of mechanical load on integrin subunits alpha5 and beta1 in chondrocytes from mature and immature cartilage explants.
abstract: Articular cartilage is subjected to cyclic compressive stresses during joint loading. There is increasing experimental evidence that this loading is essential for the chondrocytes to maintain the functionality of the cartilage extracellular matrix (ECM) and that members of the integrin family of transmembrane receptors may play an important role in signal mechanotransduction between the ECM and chondrocytes. Of particular interest are the integrin subunits alpha5 and beta1, which are known to form the receptor for fibronectin, an important ECM protein, and to be involved in mechanotransduction as well as in the regulation of cytokine production. In this study, we measured the amounts of the integrin subunits alpha5 and beta1 in chondrocytes from young (immature) and adult (mature) bovine articular cartilage explants which were subjected to a continuously applied cyclic compressive stress of 1 MPa for 6 and 24 h. The integrin content per chondrocyte was measured immediately after load cessation by flow cytometry following matrix digestion to release the cells. We found that a mechanical stress induced an increase in the number of integrin subunit alpha5 in immature and mature cartilage but not in the integrin subunit beta1 content. The integrin contents were greatest after 6 h of loading and returned to control levels after 24 h of unloading. The results of this study supply further experimental evidence that chondrocytes respond to changes in their mechanical environment and that the integrin alpha5beta1 may act as a mechanical signal transducer between the chondrocyte and the ECM for the modulation of cellular physiology.
authors.affiliation: Laboratory for Soft Tissue Research, Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, USA. eliana.zaugg@pharma.unizh.ch
authors.forename: Eliana
authors.initials: E
authors.lastname: Lucchinetti
authors.forename: Madhu M
authors.initials: MM
authors.lastname: Bhargava
authors.forename: Peter A
authors.initials: PA
authors.lastname: Torzilli
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D019902:Chondrocytes / Q000378:metabolism*; D046508:Culture Techniques; D039564:Integrin alphaV / Q000378:metabolism*; D019012:Integrin beta1 / Q000378:metabolism*; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D013264:Stifle / Q000166:cytology / Q000378:metabolism; D013314:Stress, Mechanical; D016474:Weight-Bearing / Q000502:physiology*
chemical_list: D039564:Integrin alphaV; D019012:Integrin beta1