Dose-related effects of theophylline added to modified Euro-Collins solution used for hypothermic preservation of lung.
OBJECTIVE
To investigate the dose-related effects of theophylline in prevention of ischemia-reperfusion injury of the lung.


METHODS
Experimental study.


METHODS
University hospital.


METHODS
Thirty Wistar rats.


METHODS
In experimental group 1 (G-I) (n = 5) 20 mg/L, in G-II (n = 5) 100 mg/L, in G-III (n = 5) 400 mg/L, and in G-IV (n = 5) 1000 mg/L of theophylline was added to modified Euro-Collins solution and perfused the lungs. Lungs were extracted without an ischemic period in control group 1 (C-I) and perfused with modified Euro-Collins solution in control group 2 (C-II). Lungs were kept in a hypothermic state for 6 hours and then ventilated for 30 minutes with 100% O(2).


RESULTS
Tissue levels of dien congugate (DC) and malonylaldehyde (MDA) were measured. Comparison of 6 groups revealed statistically significant differences for DC and MDA (p < 0.0001 for both comparisons). Both DC and MDA levels of C-II were found to be higher than G-III and G-IV (p = 0.008). DC and MDA levels of G-III and G-IV were significantly lower than G-I and G-II (p = 0.008 for all comparisons).


CONCLUSIONS
The results of this study showed that 400 mg/L and 1000 mg/L of theophylline added to the modified Euro-Collins solution decreased the intermediate products of lipid peroxidation. Theophylline merits further investigation in ischemia-reperfusion studies as a potentially beneficial agent.
Bostanci
Korkut
K
Department of Thoracic Surgery, Istanbul School of Medicine, Istanbul University, 81090 Kozyataği, Istanbul, Turkey.
Toker
Alper
A
Bayrak
Yusuf
Y
Dilege
Sukru
S
Aykac-Toker
Gulcin
G
Senturk
Mert
M
Tugrul
Mehmet
M
D000818:Animals; D001993:Bronchodilator Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006982:Hypertonic Solutions / Q000008:administration & dosage*; D007036:Hypothermia, Induced; D015227:Lipid Peroxidation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control; D013806:Theophylline / Q000494:pharmacology*
D001993:Bronchodilator Agents; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D008315:Malondialdehyde; D013806:Theophylline