Haplotypes of CYP3A4 and their close linkage with CYP3A5 haplotypes in a Japanese population.
In order to identify single nucleotide polymorphisms (SNPs) and haplotype frequencies of CYP3A4 in a Japanese population, the distal enhancer and proximal promoter regions, all exons, and the surrounding introns were sequenced from genomic DNA of 416 Japanese subjects. We found 24 SNPs, including 17 novel ones: two in the distal enhancer, four in the proximal promoter, one in the 5'-untranslated region (UTR), seven in the introns, and three in the 3'-UTR. The most common SNP was c.1026+12G>A (IVS10+12G>A), with a 0.249 frequency. Four non-synonymous SNPs, c.554C>G (p.T185S, CYP3A4(*)16), c.830_831insA (p.E277fsX8, (*)6), c.878T>C (p.L293P, (*)18), and c.1088 C>T (p.T363M, (*)11) were found with frequencies of 0.014, 0.001, 0.028, and 0.002, respectively. No SNP was found in the known nuclear transcriptional factor-binding sites in the enhancer and promoter regions. Using these 24 SNPs, 16 haplotypes were unambiguously identified, and nine haplotypes were inferred by aid of an expectation-maximization-based program. In addition, using data from 186 subjects enabled a close linkage to be found between CYP3A4 and CYP3A5 SNPs, especially among the SNPs at c.1026+12 in CYP3A4 and c.219-237 (IVS3-237, a key SNP site for CYP3A5(*)3), c.865+77 (IVS9+77) and c.1523 in CYP3A5. This result suggested that CYP3A4 and CYP3A5 are within the same gene block. Haplotype analysis between CYP3A4 and CYP3A5 revealed several major haplotype combinations in the CYP3A4-CYP3A5 block. Our findings provide fundamental and useful information for genotyping CYP3A4 (and CYP3A5) in the Japanese, and probably Asian populations.
Fukushima-Uesaka
Hiromi
H
Project Team for Pharmacogenetics, National Institute of Health Sciences, Tokyo, Japan.
Saito
Yoshiro
Y
Watanabe
Hidemi
H
Shiseki
Kisho
K
Saeki
Mayumi
M
Nakamura
Takahiro
T
Kurose
Kouichi
K
Sai
Kimie
K
Komamura
Kazuo
K
Ueno
Kazuyuki
K
Kamakura
Shiro
S
Kitakaze
Masafumi
M
Hanai
Sotaro
S
Nakajima
Toshiharu
T
Matsumoto
Kenji
K
Saito
Hirohisa
H
Goto
Yu-ichi
Y
Kimura
Hideo
H
Katoh
Masaaki
M
Sugai
Kenji
K
Minami
Narihiro
N
Shirao
Kuniaki
K
Tamura
Tomohide
T
Yamamoto
Noboru
N
Minami
Hironobu
H
Ohtsu
Atsushi
A
Yoshida
Teruhiko
T
Saijo
Nagahiro
N
Kitamura
Yutaka
Y
Kamatani
Naoyuki
N
Ozawa
Shogo
S
Sawada
Jun-ichi
J
D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005787:Gene Frequency; D008040:Genetic Linkage; D006239:Haplotypes; D006801:Humans; D007564:Japan; D020641:Polymorphism, Single Nucleotide
D003577:Cytochrome P-450 Enzyme System; C104464:CYP3A protein, human; C510164:CYP3A5 protein, human; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human