Correlations between pulp properties of eucalyptus clones and leaf volatiles using automated solid-phase microextraction.
Analysis of biogenic volatile organic compounds (BVOC) of 14 Eucalyptus clones has been performed using an automated headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography (GC)/ion trap mass spectrometry (ITMS) method. Correlations between pulp properties of Eucalyptus clones and the BVOC of their leaf headspaces were studied. The compounds alpha-terpineol and the sesquiterpene beta-eudesmol were positively correlated with S5, a property related to the hemicelluose content in the pulp. Qualitative results obtained with automated HS-SPME were sufficient to group together the same species and related hybrids through cluster analysis and were confirmed through principal component analysis. A preliminary separation of the essential oils of Eucalyptus dunnii through comprehensive two-dimensional gas chromatography (GC x GC) showed approximately 580 peaks compared to approximately 60 in a typical GC/ITMS first-dimension chromatogram. The potential of HS-SPME coupled to GC x GC to improve the separation of Eucalyptus volatiles and other plant essential oils looks extremely promising for new applications of unsupervised learning methods.
Zini
Cláudia A
CA
Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
De Assis
Teotônio F
TF
Ledford
Edward B
EB
Dariva
Claudio
C
Fachel
Jandyra
J
Christensen
Eva
E
Pawliszyn
Janusz
J
D001322:Autoanalysis; D001947:Breeding; D002849:Chromatography, Gas; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D010944:Plants / Q000737:chemistry* / Q000235:genetics*; D012641:Selection, Genetic; D045787:Sesquiterpenes, Eudesmane / Q000032:analysis; D013729:Terpenes / Q000032:analysis
D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D045787:Sesquiterpenes, Eudesmane; D013729:Terpenes; C016775:alpha-terpineol; C051082:beta-eudesmol