title: [Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts].
abstract: OBJECTIVE
  To study DNA damage of three kinds of gasoline oxygenates.
  
  
  METHODS
  Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts.
  
  
  RESULTS
  In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative.
  
  
  CONCLUSIONS
  Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
authors.affiliation: National Engine Combustion Laboratory, Tianjin University, Tianjin 300072, China.
authors.forename: Chonglin
authors.initials: C
authors.lastname: Song
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
authors.forename: Keming
authors.initials: K
authors.lastname: Liu
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D005561:Formates / Q000633:toxicity*; D008738:Methyl Ethers / Q000633:toxicity*; D051379:Mice
chemical_list: D005561:Formates; D008738:Methyl Ethers; C043243:methyl tert-butyl ether; D000431:Ethanol; C023025:methyl carbonate