Semi-quantitative expression analysis of ephrin mRNAs in the deafferented hippocampus.
To search for gene expression changes probably responsible for deafferentation-induced reorganization in the brain, we have analyzed the expression of mRNAs for ephrin-A1, -A2, -A3, -A5 and -B1 in the rat hippocampus following transection of the entorhinal afferents by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). We found that their expression in the deafferented hippocampus increased significantly by 7 days, reached the maximum at 14 days and almost recovered to control levels by 60 days post-lesion. It is notable that the up-regulation of ephrin mRNAs occurs during the reorganization in the deafferented hippocampus, suggesting that ephrins may be involved in the plasticity events of the adult brain after lesion.
Wang
Yan
Y
Key Laboratory of Neurobiology, Shanghai Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Ying
Guoxin
G
Liu
Xin
X
Zhou
Changfu
C
D000704:Analysis of Variance; D000818:Animals; D015152:Blotting, Northern; D002422:Causalgia; D003714:Denervation / Q000379:methods; D036342:Ephrins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007839:Functional Laterality; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism*; D017403:In Situ Hybridization; D009473:Neuronal Plasticity; D019580:Perforant Pathway; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D036342:Ephrins; D012333:RNA, Messenger; D040121:Semaphorin-3A