title: The induction of gene mutations and micronuclei by oxiranes and siloranes in mammalian cells in vitro.
abstract: Oxiranes and siloranes are candidate molecules for the development of composite materials with low shrinkage. Since some of these molecules are highly reactive, they could lead to adverse biological effects from underlying genetic mechanisms. Therefore, we analyzed the formation of micronuclei (chromosomal aberrations) and the induction of gene mutations (HPRT assay) in mammalian cells. The numbers of micronuclei induced by the oxirane di(cyclohexene-epoxidemethyl)ether (Eth-Ep) at low concentrations (10 micro M) were about five-fold higher than controls. The related compound epoxy cyclohexyl methyl-epoxy cyclo-hexane carboxylate (Est-Ep) was less effective. The activity of diglycidylether of bisphenol A (BADGE) was even lower but similar to the most reactive silorane, di-3,4-epoxy cyclohexylmethyl-dimethyl-silane (DiMe-Sil). No induction of micronuclei was detected in the presence of a rat liver homogenate (S9). Est-Ep and Eth-Ep also induced gene mutations. Our analyses indicated low mutagenic potentials of siloranes; however, some oxiranes induced strong effects at two genetic endpoints.
authors.affiliation: Department of Operative Dentistry and Periodontology, University of Regensburg, D-93042 Regensburg, Germany. Helmut.schweikl@klinik.uni-regensburg.de
authors.forename: H
authors.initials: H
authors.lastname: Schweikl
authors.forename: G
authors.initials: G
authors.lastname: Schmalz
authors.forename: W
authors.initials: W
authors.lastname: Weinmann
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002273:Carcinogens / Q000633:toxicity; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003510:Cyclohexanes / Q000633:toxicity; D003764:Dental Materials / Q000633:toxicity*; D004852:Epoxy Compounds / Q000633:toxicity*; D005027:Ethylene Oxide / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D007041:Hypoxanthine Phosphoribosyltransferase / Q000187:drug effects / Q000235:genetics; D008110:Liver Extracts / Q000494:pharmacology; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000235:genetics; D009154:Mutation / Q000187:drug effects* / Q000235:genetics; D051381:Rats; D012821:Silanes / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002273:Carcinogens; D003510:Cyclohexanes; D003764:Dental Materials; D004852:Epoxy Compounds; D005027:Ethylene Oxide; D007041:Hypoxanthine Phosphoribosyltransferase; D008110:Liver Extracts; D012821:Silanes; C470248:di-(3,4-epoxycyclohexylmethyl)dimethylsilane; C470249:epoxy cyclohexyl methyl-epoxy cyclo-hexane carboxylate; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds