Overexpression of LRP12, a gene contained within an 8q22 amplicon identified by high-resolution array CGH analysis of oral squamous cell carcinomas.
Chromosome 8q amplification is a common event observed in cancer. In this study, we used high-resolution array comparative genomic hybridization to resolve two neighboring regions on 8q that are both amplified in oral cancer. One region (at 8q24) contains the MYC oncogene, which is frequently overexpressed in many cancers, while the other region (at 8q22) represents a novel amplicon. The alignment of array comparative genomic hybridization profiles of 20 microdissected oral squamous cell carcinomas (OSCCs) revealed a approximately 5 Mbp region of frequent copy number alteration. This region harbors 16 known genes. Gene expression analysis comparing 15 microdissected OSCC with 16 normal epithelium samples revealed overexpression specific to LRP12 but not the neighboring genes, dihydropyrimidinase and FOG2, suggesting that LRP12 may function as an oncogene in oral tumors.
Garnis
Cathie
C
British Columbia Cancer Research Centre, and Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada. cgarnis@bccancer.bc.ca
Coe
Bradley P
BP
Zhang
Lewei
L
Rosin
Miriam P
MP
Lam
Wan L
WL
D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002898:Chromosomes, Human, Pair 8; D005784:Gene Amplification; D018628:Gene Dosage; D016259:Genes, myc; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011230:Precancerous Conditions / Q000235:genetics
D014408:Biomarkers, Tumor; C483993:LRP12 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1