[Quantity estimation of the E2/E6 HPV gene products ratio can be a prognostic marker for the stage of the cervical cancer carcinogenesis].
Cervical cancerogenesis is presently considered to be connected with human papillomavirus (HPV) infection of cervical epithelial cells. Genomic integration of an HPV DNA into epithelial cells is a probable factor increasing cervical carcinoma incidence.


OBJECTIVE
Of the study was to evaluate the calculated ratios of HPV 16 gene amplification products with multiplex PCR. We estimated quantitive ratios of HPV 16 genes: E6, E1, E2, and L1.


METHODS
Ninety three cervical carcinoma cases were included into the analysis after detecting DNA HPV16 presence in primary lesion. We isolated DNA from cervical smears.


RESULTS
We discovered statistically significant difference in low E2/E6 (< 0.4) amplification ratio patients staged IIa and in stages more advanced than IIa (p = 0.03). Moreover, we observed high E2/E6 amplification ratio in patients negative for lymph-node metastases (according to postoperative pathological report).
Łukaszuk
Krzysztof
K
Zakładu Endokrynologii Ginekologicznej, Instytut Połoznictwa i Chorób Kobiecych, AM, Pracowni Biologii Molekularnej, Niepubliczny Zakład Opieki Zdrowotnej INVICTA w Gdańsku.
Liss
Joanna
J
Emerich
Janusz
J
Wójcikowski
Czesław
C
D014408:Biomarkers, Tumor / Q000032:analysis*; D036022:Capsid Proteins; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000235:genetics; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D012097:Repressor Proteins; D012307:Risk Factors; D014412:Tumor Virus Infections / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000821:virology
D014408:Biomarkers, Tumor; D036022:Capsid Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C093478:E1 protein, Human papillomavirus 16; C054855:E2 protein, Human papillomavirus type 16; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; C063317:L1 protein, Human papillomavirus type 16