Expression of transcripts encoding AMPA receptor subunits and associated postsynaptic proteins in the macaque brain.
Glutamate is the primary excitatory neurotransmitter in the central nervous system, regulating numerous cellular signaling pathways and controlling the excitability of central synapses both pre- and postsynaptically. Localization, cell surface expression, and activity-dependent regulation of glutamate receptors in both neurons and glia are performed and maintained by a complex network of protein-protein interactions associated with targeting, anchoring, and spatially organizing synaptic proteins at the cell membrane. Using in situ hybridization, we examined the expression of transcripts encoding the AMPA receptor subunits (GluR1-GluR4) and a family of AMPA-related intracellular proteins. We focused on PDZ-proteins that are involved in the regulated pool and anchoring AMPA subunits to the cell membrane (PICK1, syntenin), and those maintaining the constitutive pool of AMPA receptors at the glutamatergic synapse (NSF, stargazin). In addition, we studied a fifth protein, KIAA1719, with high homology to the rat PDZ protein ABP, associated with the clustering of AMPA receptors at the glutamate synapse. The AMPA subunits showed significant differences in regional expression, especially in the neocortex, thalamus, striatum, and cerebellum. The expression of other proteins, even those related to a specific AMPA subunit (such as ABP and PICK1 to GluR2 and GluR3), often had different distributions, whereas others (like NSF) are ubiquitously distributed in the brain. These results suggest that AMPA subunits and related intracellular proteins are differentially distributed in the macaque brain, and in numerous structures there are significant mismatches, suggesting additional functional properties of the associated intracellular proteins..
Beneyto
Monica
M
Mental Health Research Institute and Department of Psychiatry, University of Michigan, Ann Arbor, Michigan 48109, USA. mbeneyto@umich.edu
Meador-Woodruff
James H
JH
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D003598:Cytoskeletal Proteins; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008254:Macaca nemestrina / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D018091:Receptors, AMPA / Q000235:genetics*; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D013570:Synaptic Membranes / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics; D053676:Syntenins; D033921:Vesicular Transport Proteins
C491912:CACNG2 protein, human; C491911:Cacng2 protein, rat; D015220:Calcium Channels; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C498298:PICK1 protein, rat; D021122:Protein Subunits; D012333:RNA, Messenger; D018091:Receptors, AMPA; C109621:SDCBP protein, human; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D053676:Syntenins; D033921:Vesicular Transport Proteins; D018698:Glutamic Acid