Determination of amino acids in fodders and raw materials using capillary zone electrophoresis.
Two schemes were offered for analysis of amino acid contents in fodders and raw materials for mixed fodders by capillary zone electrophoresis (CZE). The first variant provides express analysis of four technologically important amino acids (lysine, methionine, threonine, cystine) in borate buffer on characteristic absorption of aminogroup (190 nm), with limits of quantitation being on average 0.2%. The second scheme includes pre-capillary derivatization of amino acids using phenylisothiocyanate (PITC) and separation of phenylthiocarbamyl (PTC)-derivatives obtained by CZE with a detection on 254 nm, which allows to widen a list of detectable components up to 19 (without tryptophan) and significantly improve detection limits down to 0.01%. Acid hydrolysis was used for a sample preparation. The results of analysis of fodders were compared using such methods, as CZE, ion exchange chromatography (amino acid analyzer) and reversed-phase (RP)-HPLC (with gradient technique of elution).
Komarova
N V
NV
Lumex Ltd., Moskovsky Prospekt 19, St. Petersburg 198005, Russia. knv@lumex.ru
Kamentsev
J S
JS
Solomonova
A P
AP
Anufrieva
R M
RM
D000596:Amino Acids / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary; D005394:Fish Flour / Q000032:analysis; D006368:Helianthus / Q000737:chemistry; D006868:Hydrolysis; D007202:Indicators and Reagents; D017879:Isothiocyanates; D010944:Plants / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000737:chemistry
D000596:Amino Acids; D007202:Indicators and Reagents; D017879:Isothiocyanates; D011506:Proteins; D013861:Thiocyanates; C005441:phenylisothiocyanate