Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats.
OBJECTIVE
To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats.


METHODS
Male Sprague-Dawley rats with 30% total body surface area (TBSA) III degree scald were employed as the model. In this study rhGH was used at the dose of 2 IU/kg(-1)d(-1). An everted sleeve of intestine 4 cm long obtained from mid-jejunum was securely incubated in Kreb's solution with radioactive dipeptide (3H-glycylsarcosine, 3H-Gly-Sar, 10 microCi/ml) at 37 degrees C for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald rats.


RESULTS
Abundant blood supply to intestine and mesentery was observed in normal and scald rats administered rhGH, while less supply of blood to intestine and mesentery was observed in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P=0.1926), while the uptake was significantly increased (P=0.0253). The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P=0.0082, 0.0391).


CONCLUSIONS
Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH.
Sun
Bing-Wei
BW
Department of General Surgery, School of Medicine, Nanjing University, Nanjing 210002, Jiangsu Province, China. sunbinwe@hotmail.com
Zhao
Xiao-Chen
XC
Wang
Guang-Ji
GJ
Li
Ning
N
Li
Jie-Shou
JS
D000818:Animals; D001692:Biological Transport; D002056:Burns / Q000473:pathology* / Q000503:physiopathology; D002352:Carrier Proteins / Q000378:metabolism*; D004151:Dipeptides / Q000378:metabolism / Q000493:pharmacokinetics; D019382:Human Growth Hormone / Q000494:pharmacology; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D000074011:Peptide Transporter 1; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D027981:Symporters
D002352:Carrier Proteins; D004151:Dipeptides; D000074011:Peptide Transporter 1; C082186:SLC15A1 protein, human; C489940:Slc15a1 protein, rat; D027981:Symporters; D019382:Human Growth Hormone; C004194:glycylsarcosine