title: Effect of wine yeast monoculture practice on the biodiversity of non-Saccharomyces yeasts.
abstract: OBJECTIVE
  The objective of this work was to study the effect of the use of Saccharomyces cerevisiae monocultures over the biodiversity of non-Saccharomyces yeasts in wine-producing areas in Chile.
  
  
  RESULTS
  Microvinifications were carried out with grape musts of two areas. In one of them, the fermentation is carried out mainly in a spontaneous manner, whereas in the other the musts are inoculated with commercial yeasts. The isolated yeasts were identified by the internal transcribed (ITS)/restriction fragment length polymorphism technique. In the industrial production area less variability of yeast genera was observed as compared with the traditional area, an observation that is greatest at the end of the fermentation. Furthermore, a study of the production of extracellular enzymes was done. The majority of the yeasts showed at least one of the activities assayed with the exception of beta-glycosidase.
  
  
  CONCLUSIONS
  The results suggest that in the industrialized area the diversity of yeasts is less in the traditional area. Likewise, the potentiality of the non-Saccharomyces yeasts as enzyme producers with industrial interest has been confirmed.
  
  
  CONCLUSIONS
  This study shows the negative effect of the use of monocultures over the biodiversity of yeasts in wine-producing regions.
authors.affiliation: Centro de Estudios en Ciencia y Tecnología de los Alimentos (CECTA), Universidad de Santiago de Chile, Chile. aganga@lauca.usach.cl
authors.forename: M A
authors.initials: MA
authors.lastname: Ganga
authors.forename: C
authors.initials: C
authors.lastname: Martínez
mesh_terms: D000681:Amylases / Q000378:metabolism; D044822:Biodiversity; D002480:Cellulase / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods; D016533:Mycological Typing Techniques; D012441:Saccharomyces cerevisiae / Q000502:physiology; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000201:enzymology / Q000502:physiology*
chemical_list: D000681:Amylases; D002480:Cellulase; D010450:Endopeptidases