[Influence of decoction methods and compatibility on content of cinnamaldehyde in Mahuang decoction].
OBJECTIVE
To develop a GC-MS method for the determination of cinnamaldehyde and study on the influence of decoction methods and compatibility on the contents of cinnamaldehyde in Mahuang decoction.


METHODS
Gas chromatography-mass spectrometry (GC-MS) was used. Chromatography/mass spectrometric conditions were: capillary column DB-17 (30 m x 0.5 mm, 0.25 micron), helium as carrier gas, non-split injection, column primary temperature 120 degrees C, 120 degrees C 8 degrees C/min-->250 degrees C, with 1.0 ml/min runoff, and injection volume 1 microliter. The results were analyzed by one-way ANOVA (SPSS).


RESULTS
The calibration curves were linear in the range from 0.0136 mg/ml to 0.136 mg/ml (r = 0.9999). The RSD of precison, stability and reproducibilities were 1.02% (n = 6), 2.57% in 24 h and 4.20%, respectively. The cinnamaldehyde content in the blended Mahuangtang decoction (BMHD) was significantly lower than the one in the separated and mixtured decoction and Ramulus cinnamomi decoction (P < 0.05). The separated and mixtured decoction was higher than Ramulus cinnamomi decoction (P > 0.05).


CONCLUSIONS
The main influencing factor of the cinnamaldehyde content was the compatibility. This method is precise and reliable to study cinnamaldehyde in Mahuang Decoction.
Wei
Fenghuan
F
First Military Medical University, Guangzhou 510515.
Luo
Jiabo
J
Chen
Feilong
F
Wang
Yonggang
Y
D000171:Acrolein / Q000031:analogs & derivatives* / Q000737:chemistry*; D027424:Cinnamomum / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D029790:Ephedra sinica / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013678:Technology, Pharmaceutical / Q000379:methods*
D004365:Drugs, Chinese Herbal; D000171:Acrolein; C012843:cinnamaldehyde