title: Luminescence spectroscopic studies of trimethinecyanines substituted in polymethine chain with nucleic acids and proteins.
abstract: The series of symmetrical beta-substituted and alpha,gamma-substituted trimethinecyanine dyes were studied for their absorption and fluorescent characteristics in unbound state and in the presence of nucleic acids and proteins. It was shown that beta-substituted and alpha,gamma-bridged trimethinecyanines containing extended heterocyclic systems or N-phenyl as well as N-cyclohexyl substituents demonstrate increased affinity to proteins. At the same time the presence of both N-phenyl and N-cyclohexyl substituents leads to the decrease of the dye fluorescence intensity in complexes with nucleic acids. For trimethinecyanines similarly to unsymmetrical monomethines the presence of N-omega-hydroxy alkyl substituents results in the increase of fluorescence intensity of dye-DNA complex and the emission decrease of dye-RNA complex.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
authors.forename: V B
authors.initials: VB
authors.lastname: Kovalska
authors.forename: M Yu
authors.initials: MY
authors.lastname: Losytskyy
authors.forename: S M
authors.initials: SM
authors.lastname: Yarmoluk
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D004126:Dimethylformamide / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D049449:Luminescence; D008956:Models, Chemical; D008958:Models, Molecular; D009696:Nucleic Acids / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D002232:Carbocyanines; D004396:Coloring Agents; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012709:Serum Albumin; D012313:RNA; D004126:Dimethylformamide