title: Effects of the molecular properties of mixed solvents on the elution of alkyl benzoates in RPLC.
abstract: The retention behavior and mechanism of methyl, ethyl, propyl, isopropyl, buthyl and isobuthyl benzoates have been studied at different eluent compositions of aqueous mixtures with water-soluble organic solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile (AN), 1,4-dioxane and tetrahydrofuran (THF)) in RPLC. The retention of the solutes is discussed based on the solvent composition, solvent polarity (ETN value), preferential solvation, hydrogen bonding and solvent clusters of the eluents. The smaller ETN values and the larger preferential solvation of the mixed solvent eluted the solutes faster. The IR spectra of HDO suggested that the solvents, except for methanol and ethanol, break the hydrogen bonding between water molecules, resulting in fast elution of the solutes. Based upon the results, we chose an optimum solvent composition for the separation of benzoates and applied it to the determination of the benzoates in clove.
authors.affiliation: Department of Health and Nutrition Science, Faculty of Health and Social Welfare Science, Nishikyushu University, Kanzaki-machi, Saga 842-8585, Japan. midori@nisikyu-u.ac.jp
authors.forename: Midori
authors.initials: M
authors.lastname: Yasuda
authors.forename: Tamiyoshi
authors.initials: T
authors.lastname: Sonda
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hiraoka
authors.forename: Aki
authors.initials: A
authors.lastname: Horita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tabata
mesh_terms: D001565:Benzoates / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D015386:Hazardous Substances / Q000032:analysis; D006860:Hydrogen Bonding; D012997:Solvents / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry
chemical_list: D001565:Benzoates; D015386:Hazardous Substances; D012997:Solvents