title: Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus.
abstract: Pigs were immunised with recombinant BCG (rBCG) expressing a truncated form of GP5 (lacking the first 30 NH(2)-terminal residues) (rBCGGP5) and M protein (rBCGM) of porcine reproductive and respiratory syndrome virus (PRRSV). At 30 days post-inoculation (dpi), pigs inoculated with rBCGGP5 and rBCGM developed a specific humoral immune response against the viral proteins, as detected by commercial ELISA and Western blot tests, and at 60 dpi, three out of five animals developed neutralizing antibodies with titers ranging from 1:4 to 1:8. At 67 dpi, an IFN-gamma response against BCG antigens, but not against the viral proteins, was detected by ELISPOT in inoculated pigs. Following challenge with a pathogenic strain of PRRSV, pigs inoculated with rBCG showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Reginaldo G
authors.initials: RG
authors.lastname: Bastos
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: Raúl G
authors.initials: RG
authors.lastname: Barletta
authors.forename: Allan R
authors.initials: AR
authors.lastname: Doster
authors.forename: Eric
authors.initials: E
authors.lastname: Nelson
authors.forename: Federico
authors.initials: F
authors.lastname: Zuckermann
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Osorio
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation / Q000276:immunology; D001500:BCG Vaccine / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D001831:Body Temperature; D004797:Enzyme-Linked Immunosorbent Assay; D007111:Immunity, Cellular / Q000276:immunology; D008198:Lymph Nodes / Q000821:virology; D014066:Palatine Tonsil / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000276:immunology* / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000276:immunology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*; D019562:Viral Load; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001500:BCG Vaccine; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C114789:glycoprotein 5, PRRSV