Effects of different dietary oils on inflammatory mediator generation and fatty acid composition in rat neutrophils.
Virgin olive oil (VOO) compared with fish oil (FO) and evening primrose oil (PO) on the ability of stimulated leukocytes to produce inflammatory mediators was investigated in rats. Weaned Wistar rats were fed a basal diet (BD) (2% by weight of corn oil) or diets containing 15% by weight of VOO, PO, or FO. After 8 weeks, glycogen-elicited peritoneal polymorphonuclear leukocytes, mainly neutrophils, were isolated. The calcium-ionophore stimulated neutrophils (2.5 x 10(6) cells/mL) obtained from rats fed the different oils produced a higher release of lysosomal enzymes (beta-glucuronidase, lysozyme, and myeloperoxidase [MPO]) compared with those fed BD. The production of reactive oxygen species (ROS) in response to the stimulant, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), by neutrophils from the VOO group (15.44 nmol of O(2)(-) and 6.56 nmol of H(2)O(2)) was similar to the BD group (12.01 nmol O(2)(-) and 8.49 nmol H(2)O(2)) and significantly lower than the PO (20.90 nmol O(2)(-) and 10.84 nmol H(2)O(2)) and FO (20.93 nmol O(2)(-) and 12.79 nmol H(2)O(2)) groups. The cyclooxygenase-derived eicosanoid production was reduced by the lipid enrichment of the diets. Whereas the generation of prostaglandin E(2) (PGE(2)) was significantly decreased in VOO (5.40 ng/mL), PO (4.95 ng/mL), and FO (1.44 ng/mL) groups compared with BD (8.19 ng/mL), thromboxane B(2) (TXB(2)) reduction was especially significant in neutrophils from the FO diet group (14.67 ng/mL compared with 26.69 ng/mL from BD). These experimental data suggest that FO and PO, as well as VOO, could be considered a valuable strategy in preventing the generation of some inflammatory mediators.
de La Puerta Vázquez
R
R
Departamento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain.
Martínez-Domínguez
E
E
Sánchez Perona
J
J
Ruiz-Gutiérrez
V
V
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D000001:Calcimycin / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D015777:Eicosanoids / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis*; D005228:Fatty Acids, Essential / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism; D006003:Glycogen / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D019787:Linoleic Acid / Q000032:analysis; D008041:Linoleic Acids; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism*; D031665:Oenothera biennis; D019301:Oleic Acid / Q000032:analysis; D000069463:Olive Oil; D010537:Peritoneum / Q000166:cytology; D009195:Peroxidase / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood; D017965:gamma-Linolenic Acid / Q000032:analysis
D004042:Dietary Fats, Unsaturated; D015777:Eicosanoids; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D005395:Fish Oils; D008041:Linoleic Acids; D000069463:Olive Oil; D010938:Plant Oils; D017382:Reactive Oxygen Species; D013481:Superoxides; D016718:Arachidonic Acid; D019301:Oleic Acid; D000001:Calcimycin; C028498:evening primrose oil; D013929:Thromboxane B2; D017965:gamma-Linolenic Acid; D006003:Glycogen; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase; D009113:Muramidase; D005966:Glucuronidase; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate