title: Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay.
abstract: A fully automated chemiluminescent microparticle immunoassay (Architect HBsAg QT) was used for the detection and quantitation of hepatitis B surface antigen (HBsAg). The assay is capable of processing up to 800 HBsAg tests per hour. The concentration of HBsAg is determined by utilizing a previously generated Architect HBsAg calibration curve. Architect HBsAg QT sensitivity was found to be around 0.2ng/ml which is equivalent or superior to other known and commercially available enzyme immunoassays and/or chemiluminescent immunoassays. We performed a quantitative study of HBsAg, HBeAg, HBV-DNA and HBV-DNA polymerase in over 733 sera obtained from 43 chronic hepatitis B carriers. Serum HBsAg levels detected by Architect HBsAg QT were found to be higher in HBeAg-positive than in anti-HBe-positive HBV chronic carriers and correlated with the level of serum HBV-DNA and HBV-DNA polymerase.
authors.affiliation: The Laboratory for Clinical Investigation, Osaka University Hospital, 2-15 Yamadaoka, Suita-shi, 565-0871, Osaka, Japan. exit@hp-lab.med.osaka-u.ac.jp
authors.forename: Matsuo
authors.initials: M
authors.lastname: Deguchi
authors.forename: Naoko
authors.initials: N
authors.lastname: Yamashita
authors.forename: Masanori
authors.initials: M
authors.lastname: Kagita
authors.forename: Seishi
authors.initials: S
authors.lastname: Asari
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
authors.forename: Takahiko
authors.initials: T
authors.lastname: Tsuchida
authors.forename: Kazushige
authors.initials: K
authors.lastname: Iinuma
authors.forename: Isa K
authors.initials: IK
authors.lastname: Mushahwar
mesh_terms: D001331:Automation; D002353:Carrier State / Q000821:virology; D004279:DNA, Viral / Q000097:blood; D004259:DNA-Directed DNA Polymerase / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006513:Hepatitis B e Antigens / Q000097:blood; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D008863:Microspheres; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D004259:DNA-Directed DNA Polymerase