Effects of Azadirachta indica extract on gastric ulceration and acid secretion in rats.
The effect of Azadirachta indica extract on gastric ulceration was studied in albino rats. Azadirachta indica extract (100-800 mg/kg p.o., 100-25 mg/kg i.p.) significantly inhibited gastric ulceration induced by indomethacin (40 mg/kg). Administration of 800 mg/kg p.o. and 250 mg/kg i.p. caused 100% cytoprotection against indomethacin (40mg/kg, i.p.)-induced gastric ulceration. This action was accompanied by a dose-dependent decrease in total gastric acidity. In order to investigate the probable mechanism of Azadirachta indica antiulcer activity, the effect of the extract alone and in combination with histamine (1mg/kg) and cimetidine (0.12 mg/kg) on gastric acid secretion in situ was studied. Azadirachta indica (250 mg/kg) significantly inhibited the basal and histamine-induced gastric acid secretion. Cimetidine seemed to augment Azadirachta indica inhibition of gastric acid secretion. The results suggest that the stem bark extract of Azadirachta indica possesses antiulcer agents, which probably act via histamine H(2) receptor.
Raji
Yinusa
Y
Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria. raji_ui@yahoo.com
Ogunwande
Isiaka Ajani
IA
Osadebe
Caleb Amaechi
CA
John
Godwin
G
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use* / Q000633:toxicity; D031601:Azadirachta; D002927:Cimetidine / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D005744:Gastric Acid / Q000378:metabolism*; D006632:Histamine / Q000627:therapeutic use; D007213:Indomethacin; D008297:Male; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000627:therapeutic use / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D023382:Toxicity Tests, Acute
D000897:Anti-Ulcer Agents; D010936:Plant Extracts; D002927:Cimetidine; D006632:Histamine; D007213:Indomethacin