Mitochondrial DNA heteroplasmy in calves cloned by using adult somatic cell.
Adult somatic cell cloned calves were produced by somatic cell nuclear transfer prepared by fusion of cultured ear fibroblast from a Holstein cow into enucleated oocytes of Luxi Yellow cow. In order to determinate the source of mitochondrial DNA of cloned calves, we designed the breed-specific PCR primers by aligning the known D-loop sequences of Bos taurus and analyzed the displacement loop sequences of five live cloned calves by breed-specific primers PCR. The results demonstrated that mtDNA originated from Holstein breed and that from Luxi breed co-exist in all five live calves.
Han
Zhi-Ming
ZM
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Chen
Da-Yuan
DY
Li
Jin-Song
JS
Sun
Qing-Yuan
QY
Wan
Qiu-Hong
QH
Kou
Zhao-Hui
ZH
Rao
Gang
G
Lei
Lei
L
Liu
Zhong-Hua
ZH
Fang
Sheng-Guo
SG
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D019976:Cloning, Organism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D053652:Nuclear Transfer Techniques; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D004272:DNA, Mitochondrial