Dual activation of the Drosophila toll pathway by two pattern recognition receptors.
The Toll-dependent defense against Gram-positive bacterial infections in Drosophila is mediated through the peptidoglycan recognition protein SA (PGRP-SA). A mutation termed osiris disrupts the Gram-negative binding protein 1 (GNBP1) gene and leads to compromised survival of mutant flies after Gram-positive infections, but not after fungal or Gram-negative bacterial challenge. Our results demonstrate that GNBP1 and PGRP-SA can jointly activate the Toll pathway. The potential for a combination of distinct proteins to mediate detection of infectious nonself in the fly will refine the concept of pattern recognition in insects.
Gobert
Vanessa
V
Unité Propre de Recherche 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, F67084 Strasbourg Cedex, France.
Gottar
Marie
M
Matskevich
Alexey A
AA
Rutschmann
Sophie
S
Royet
Julien
J
Belvin
Marcia
M
Hoffmann
Jules A
JA
Ferrandon
Dominique
D
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017344:Genes, Insect; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006094:Gram-Positive Bacteria / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism; D006999:Hypocreales / Q000502:physiology; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors
D002352:Carrier Proteins; D004251:DNA Transposable Elements; C054756:DptA protein, Drosophila; D029721:Drosophila Proteins; C480085:Gnbp1 protein, Drosophila; D019476:Insect Proteins; D011956:Receptors, Cell Surface; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors; C118897:peptidoglycan recognition protein; C086179:spz protein, Drosophila; C091227:DRS protein, Drosophila; D012697:Serine Endopeptidases; C460880:psh protein, Drosophila