Chromosome positional effects of gene expressions after cellular senescence.
Normal human fibroblasts stop dividing after a limited number of cell divisions termed cellular senescence. Telomere shortening has been shown to be the main factor that causes cellular senescence, however, the molecular mechanism of how telomere shortening causes cellular senescence is unclear. Here we analyze the relationship between gene expressions and their chromosomal locations during cellular senescence. It appears that the expression of genes located in chromosome 4 is preferentially altered after senescence. Moreover, we identify four chromosomal loci in which gene expressions are affected by senescence. Finally, we show that there is no preferential alteration of telomere-proximal genes during cellular senescence, implying that cellular senescence is not caused by derepression of telomere-proximal genes.
Chen
Hung-Lin
HL
Institute of Biopharmaceutical Science, National Yang-Ming University, Shih-Pai, 112 Taipei, Taiwan, ROC.
Lu
Ching-You
CY
Hsu
Yi-Hsin
YH
Lin
Jing-Jer
JJ
D002453:Cell Cycle; D002460:Cell Line; D016922:Cellular Senescence; D002894:Chromosomes, Human, Pair 4; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction; D016615:Telomere / Q000648:ultrastructure; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D018076:DNA, Complementary; D001616:beta-Galactosidase