The C289G and C418R missense mutations cause rapid sequestration of human Parkin into insoluble aggregates.
Mutations in the parkin gene are responsible for autosomal recessive parkinsonism. The disease-linked missense mutations are highly concentrated in the RING-IBR-RING domains of Parkin. In this study, we investigated the consequences of several missense parkin gene mutations in cell culture. We have demonstrated that two of these mutations (C289G and C418R), which replace consensus cysteine residues in the RING domains, significantly decrease the solubility of Parkin in cells. Upon overexpression, the presumably misfolded proteins formed cytoplasmic aggregates that concentrated into large perinuclear inclusion bodies when proteasome activity was inhibited. This process required active microtubule-dependent retrograde transport, as previously reported for aggresome formation. These results provide information on the molecular basis of the loss of function caused by mutations of critical residues in Parkin. They also contribute to our understanding of the cellular mechanism underlying the aggregation of mutant Parkin.
Gu
Wen-Jie
WJ
INSERM U 289, Hôpital de la Salpêtrière, 75013 Paris, France.
Corti
Olga
O
Araujo
Francisco
F
Hampe
Cornelia
C
Jacquier
Sandrine
S
Lücking
Christoph B
CB
Abbas
Nacer
N
Duyckaerts
Charles
C
Rooney
Thomas
T
Pradier
Laurent
L
Ruberg
Merle
M
Brice
Alexis
A
D000818:Animals; D019556:COS Cells; D003546:Cysteine Endopeptidases / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000235:genetics* / Q000378:metabolism; D046911:Macromolecular Substances; D008870:Microtubules / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D020125:Mutation, Missense / Q000235:genetics*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D046988:Proteasome Endopeptidase Complex; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000502:physiology; D012995:Solubility; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D014452:Ubiquitins / Q000378:metabolism
D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D014452:Ubiquitins; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex