[Immunoscintigraphy of anti-type IV collagenase monoclonal antibody in nude mice bearing human lung cancer xenograft].
OBJECTIVE
Matrix metalloproteinases (MMPs)play the important role in many steps of tumor growth and metastasis. Type IV collagenase, which is a key member of MMPs family, has been viewed as a promising target in tumor study. The aim of this study was to evaluate the tumor-specific distribution of the anti-type IV collagenase monoclonal antibody (mAb) 3G11 by radioimaging in tumor-bearing nude mice.


METHODS
MAb 3G11 purified by affinity chromatography was labeled with either 131- or 125- iodide by the Iodogen method. Immunoreactivity of mAb 3G11 was determined by ELISA. (131)I-labeled 3G11 was incubated in three different media at 37 Celsius degree and its in vitro stability was tested. Normal BALB/c mice were injected intravenously with 388.5 kBq per mouse of (125)I-labeled 3G11 to explore the pharmacokinetic patterns. The scintigraphic images of human lung carcinoma PG xenografts grown subcutaneously in BALB/c nude mice were made after intravenously administrating of 6.44 MBq per mouse of (131)I-labeled 3G11.


RESULTS
MAb 3G11 was more than 98% in purity via affinity purification. The immunoreactivity of mAb 3G11 decreased by approximately 10%-20% after cold iodination. Patterns of blood clearance of mAb 3G11 was defined as two-compartment model, with T(1/2alpha) and T(1/2beta) calculated to be 7.2 h and 345.2 h, respectively. (131)I-labeled 3G11 was almost stable in vitro for 72 h. A clear image of the xenografted tumor was obtained at 72 h, and it further improved at 120 h.


CONCLUSIONS
MAb 3G11 showed high specificity and affinity with tumor tissue through scintiscanning.
Dai
Yao
Y
Institute of Medicinal Biotechnology,Chinese Academy of Medical Science and Peking Union Medical College, Beijing,100050, PR China.
Jia
Bing
B
Wang
Fan
F
Du
Jin
J
Shang
Bo-Yang
BY
Zhen
Yong-Su
YS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung; D017364:Collagenases / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D007456:Iodine Isotopes; D008175:Lung Neoplasms; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D016719:Radioimmunodetection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007456:Iodine Isotopes; D017364:Collagenases