[Determination of polymorphic DNA sequences STR-PCR in decomposed human tissues].
This paper reports the possibility of DNA profiling obtained from soft tissues influenced by the decomposition process: heart, kidney, liver collected during autopsy. As a control DNA, profile from blood was determined. DNA was extracted by the phenol-chloroform method. Amplification was performed with the use of the GenePrintSTR Multiplex (CSF1PO, TPOX, TH01) system, Promega and AmpF[symbol: see text]STR Identifier, Applied Biosystems. PCR products of the CTT system were separated by electrophoresis on denaturing polyacrylamide gels and visualized by silver staining. The 16 loci of AmpF[symbol: see text]STR Identifier products were analyzed by capillary electrophoresis on an ABI PRISM 310 sequencer. The results from both methods were compared. Electrophoresis of the CTT products showed clear results for DNA extracted from blood and decomposed tissues, particularly from the heart and kidney. The capillary electrophoresis method gave a positive signal for all 16 loci of AmpF[symbol: see text]STR Identifier for DNA extracted from heart, kidney and blood. Worse results similar to the manual method were obtained for DNA extracted from the liver. The soft tissues, also decomposed by putrefaction can be a useful source of genomic DNA in personal identification and paternity testing.
Piasecka-Pazik
Dorota
D
Katedry i Zakładu Medycyny Sadowej AM w Gdańsku.
Szczerkowska
Zofia
Z
D001344:Autopsy; D001801:Blood Stains; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics; D013997:Time Factors
D004247:DNA