Pyridoxine 5'-phosphate oxidase is a candidate gene responsible for hypertension in Dahl-S rats.
To identify candidate genes responsible for hypertension in Dahl salt-sensitive rats (Dahl-S), an oligonucleotide microarray analysis was performed to find differentially expressed genes in kidneys of Dahl-S and Lewis rats. We obtained 101 F2 male rats from Dahl-S and Lewis rats and performed precise measurements of blood pressure (BP) and heart rate by telemetric monitoring at 14 weeks of age after 9 weeks of salt-loading. The correlation analysis between genotypes of differentially expressed genes and BP in F2 rats indicated that pyridoxine 5'-phosphate oxidase (Pnpo) and catecholamine-O-methyltransferease (Comt) showed a highly significant association with BP. However, in the case of Comt, the Dahl-S genotype correlated with low BP. Short/branched chain acyl-CoA dehydrogenase and Sah also showed a significant association with systolic blood pressure. The present study provided evidence that Pnpo is a candidate gene responsible for hypertension in Dahl-S rats.
Okuda
Tomohiko
T
National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
Sumiya
Toshiki
T
Iwai
Naoharu
N
Miyata
Toshiyuki
T
D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure; D015152:Blotting, Northern; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D005838:Genotype; D006339:Heart Rate; D006973:Hypertension; D007668:Kidney / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D051381:Rats; D020303:Rats, Inbred Dahl; D011917:Rats, Inbred Lew; D013686:Telemetry
D000429:Alcohol Oxidoreductases; C050708:pyridoxine 5-dehydrogenase