Intravital microscopy for the study of mouse microcirculation in anti-inflammatory drug research: focus on the mesentery and cremaster preparations.
Intravital microscopy is an extremely useful tool used as a qualitative and quantitative way of observing leukocyte-endothelial cell interactions in-vivo. This present article reviews the methods of the technique of intravital microscopy, in particular focussing on the mesentery and cremaster preparations. It focuses on how to actually carry out the experiments required to directly observe and localize the changes in the function of the microcirculation. Where necessary the reader is asked to refer to a selection of highly acclaimed publications, which should enable the reader to truly appreciate, and if necessary perform, the technique of intravital microscopy.
Gavins
Felicity N E
FN
The William Harvey Research Institute, Bart's and The Royal London, Queen Mary School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, UK. F_gavins@yahoo.co.uk
Chatterjee
Bristi E
BE
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015195:Drug Design; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D051379:Mice; D008833:Microcirculation / Q000187:drug effects; D008853:Microscopy / Q000379:methods*; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000098:blood supply*; D009131:Muscle, Smooth, Vascular / Q000098:blood supply*; D013737:Testis / Q000098:blood supply
D000893:Anti-Inflammatory Agents