[The formation of transgenic mice with hemangiomas].
OBJECTIVE
To set up the animal model of hemangioma by microinjecting the PyMT transgenic DNA.


METHODS
Constructing the transgenic PyMT gene, and microinjecting it into fertilized embryos which were transferred to pseudopregnant recipients then. Observing the phenotype of the newborn-mice, detecting the integration of transgenic DNA by PCR, and analyzing the histological morphon of the neoplasm of the mice.


RESULTS
The transgenic DNA was proved to be right and has been microinjected into 579 fertilized embryos, 62 mice were born. Within the 62 mice, one mouse was found being the phenotype of hemangioma. PyMT gene was expressed in the total DNA of the transgenic mouse by PCR.


CONCLUSIONS
It could be a good way to build animal model of hemangioma with transgenic PyMT DNA.
Xu
Qing
Q
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Zhang
Zhi-yuan
ZY
Chen
Wan-tao
WT
Yang
Wen-jun
WJ
Wang
Zhu-gang
ZG
Wang
Long
L
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology*; D005260:Female; D006391:Hemangioma / Q000209:etiology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections
D000952:Antigens, Polyomavirus Transforming