title: [A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province].
abstract: OBJECTIVE
  To identify subtypes of human immunodeficiency virus 1 (HIV-1) strains and their distribution, infection sources, and the trends of HIV infection in Jiangsu province.
  
  
  METHODS
  Anticongulated bloods from 46 infected persons were collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes. The analysis of consensus sequence, genetic distance and phylogenetic tree were conducted with GCG software.
  
  
  RESULTS
  By the end of 2001, there had been six subtypes of HIV-1 strains identified in Jiangsu province: A, B, B', C, D and E. The predominant subtypes were C (accounting for 40.48%) and B' (accounting for 38.10%). Subtype C accounted for 86.67% among injecting DUs while subtype B' accounted for 91.67% among commercial blood donors and receivers.
  
  
  CONCLUSIONS
  Subtype B'among commercial blood donors was brought to Jiangsu from neighboring provinces. The outbreak of HIV-1 infection among local DUs was caused by subtype C from Xinjiang province. Findings from HIV/AIDS molecular epidemiologic study suggest that it is challenging for Jiangsu to treat patients, apply vaccine, prevent and control AIDS in the future.
authors.affiliation: Jiangsu Province Center for Diseases Prevention and Control, Nanjing 210009, China.
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Cheng-mei
authors.initials: CM
authors.lastname: Jia
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D002681:China / Q000453:epidemiology; D015752:Genes, env / Q000235:genetics; D015658:HIV Infections / Q000097:blood* / Q000453:epidemiology; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA