Analysis of low molecular weight intracellular associations of a human mannan binding lectin (MBL).
Human mannan binding lectin (MBL) is a member of the collectins, a group of proteins that contain a dual structure with a lectin and a collagenous moieties. The collectins are considered as major actors of innate immunity. We report the presence of low molecular weight intracellular MBL forms in human hepatocytic cell lysates, with binding capacities associated to its lectin and/or its collagen moiety. Competition with D-mannose and with antibodies directed against the lectin binding site of MBL indicate that the 60 kDa form represents an intracellular association of MBL through its lectin moiety. The effects of collagenase or MBL associated serine proteases (MASPs) from a MBL deficient plasma, gave evidence that the 60 KDa form contains also collagen and suggested the binding of a ligand to this collagen part. These results show that this intracellular form of MBL shares binding properties with circulating MBL. The binding potential of the lectin and the collagenous parts of precursor forms of intracellular MBL may suggest that they behave as molecular chaperone. The complexity of MBL structure and functions deserves further investigation on other intracellular forms of MBL.
Dumestre-perard
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Laboratoire d'Immunologie, Université Joseph Fourier JE 2236, Hôpital Sud, CHU Grenoble, Avenue de Kimberley, 38130 Echirolles, France. chantal.dumestre@wanadoo.fr
Ponard
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Colomb
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D017364:Collagenases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008358:Mannose / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism
D008024:Ligands; D037601:Mannose-Binding Lectin; D012697:Serine Endopeptidases; D017364:Collagenases; D008358:Mannose