Validation of a simplified method for determination of cimetidine in human plasma and urine by liquid chromatography with ultraviolet detection.
A HPLC method was developed for determination of cimetidine in human plasma and urine. Plasma samples were alkalinized followed by liquid extraction with water-saturated ethyl acetate then evaporated under nitrogen. The extracts were reconstituted in mobile phase and injected onto a C(18) reversed-phase column; UV detection was set at 228 nm. Urine samples were diluted with an internal standard/mobile phase mixture (1:9) prior to injection. The lower limit of quantification in plasma and urine were 100 ng/ml and 10 microg/ml, respectively; intra- and inter-day coefficients of variation were </=4.2%. Advantages of this validated assay include a readily available internal standard, simplified plasma extraction and urine dilution methods, and applicability to clinical studies investigating the renal handling of cimetidine.
Iqbal
Tahira
T
Renal Clinical Pharmacology Lab, Department of Pharmacy Practice and Science, University of Maryland, School of Pharmacy, 100 N. Penn St., AHB Rm. 540-D, Baltimore, MD 21201, USA.
Karyekar
Chetan S
CS
Kinjo
Minori
M
Ngan
Gilbert C
GC
Dowling
Thomas C
TC
D000897:Anti-Ulcer Agents / Q000097:blood* / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D002927:Cimetidine / Q000097:blood* / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000897:Anti-Ulcer Agents; D002927:Cimetidine