P fimbria-specific B cell responses in patients with urinary tract infection.
Local immune response may be important in defense against urinary tract infection (UTI). P fimbria, an important virulence factor of Escherichia coli, is a noteworthy candidate for use in a vaccine against pyelonephritis (PN). Eleven patients with PN and 14 patients with lower urinary tract infection (LUTI) caused by E. coli were studied for mucosa-derived antibody-secreting cells (ASCs) and for urinary antibodies. In the 10 patients with P-fimbriated (P+) E. coli, an ASC response to P fimbria was found in 5 of 5 patients who had PN and 1 of 5 patients who had LUTI. The response to P fimbria was stronger among patients with P+ PN than among patients with PN caused by non-P-fimbriated E. coli (P-) (P<.001) or patients with P+ LUTI (P<.001). The response to P fimbria was also stronger than the response to outer-membrane protein A among all patients with PN. P fimbria-specific urinary immunoglobulin A antibody levels were higher among patients with P+ PN than those with P- PN. The results show a P fimbria-specific local immune response, which further encourages the use of P fimbria in locally administrable UTI vaccines.
Kantele
Anu
A
Division of Infectious Diseases, Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland. anu.kantele@hus.fi
Möttönen
Timo
T
Ala-Kaila
Kyösti
K
Arvilommi
Heikki S
HS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000652:urine; D001402:B-Lymphocytes / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology* / Q000517:prevention & control / Q000652:urine; D029968:Escherichia coli Proteins / Q000276:immunology; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology; D011704:Pyelonephritis / Q000276:immunology; D014552:Urinary Tract Infections / Q000276:immunology* / Q000517:prevention & control / Q000652:urine
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C078167:OMPA outer membrane proteins