[Deoxyribonuclease I gene expression in systemic lupus erythematosus patients].
OBJECTIVE
To observe whether deoxyribonuclease I (DNASE1) gene expression and its DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis. An analysis was also made to disclose whether specific single nucleotide polymorphisms (SNPs) haplotype had effects onDNASE1 gene expression and its alternatively spliced transcripts.


RESULTS
DNASE1 gene expression was higher in SLE patients than in normal controls (P<0.001), and in patients it was found to be of no relationship with SLE disease activity index score. However, it was increased in female patients. Capillary electrophoresis revealed that the pattern of alternatively spliced transcripts in patients was not the same as that in normal controls. Moreover, it seemed that different SNPs haplotype combination might show different transcript pattern in SLE patients.


CONCLUSIONS
In SLE patients, DNASE1 gene expression is abnormal and there are alternatively spliced transcripts different from those in normal controls. DNASE1 gene is a critical factor in the pathogenesis of SLE.
Feng
Xue-bing
XB
Department of Rheumatology, Affiliated Renji Hospital, Shanghai Second Medical University, Shanghai, 200001 PR China.
Shen
Nan
N
Chen
Shun-le
SL
Hua
Jin
J
Sun
Li
L
Qian
Jie
J
D000293:Adolescent; D000328:Adult; D017398:Alternative Splicing; D003850:Deoxyribonuclease I / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D003850:Deoxyribonuclease I