Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.
CHL27, the Arabidopsis homologue to Chlamydomonas Crd1, a plastid-localized putative diiron protein, is required for the synthesis of protochlorophyllide and therefore is a candidate subunit of the aerobic cyclase in chlorophyll biosynthesis. delta-Aminolevulinic acid-fed antisense Arabidopsis plants with reduced amounts of Crd1/CHL27 accumulate Mg-protoporphyrin IX monomethyl ester, the substrate of the cyclase reaction. Mutant plants have chlorotic leaves with reduced abundance of all chlorophyll proteins. Fractionation of Arabidopsis chloroplast membranes shows that Crd1/CHL27 is equally distributed on a membrane-weight basis in the thylakoid and inner-envelope membranes.
Tottey
Stephen
S
Department of Chemistry and Biochemistry, University of California, Box 951569, Los Angeles, CA 90095, USA.
Block
Maryse A
MA
Allen
Michael
M
Westergren
Tomas
T
Albrieux
Catherine
C
Scheller
Henrik V
HV
Merchant
Sabeeha
S
Jensen
Poul Erik
PE
D000622:Aminolevulinic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D015388:Organelles / Q000187:drug effects / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011521:Protochlorophyllide / Q000096:biosynthesis*; D020524:Thylakoids / Q000378:metabolism
D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D011521:Protochlorophyllide; D000622:Aminolevulinic Acid; D010088:Oxidoreductases