Enhanced post-ischaemic recovery in rabbit kidney after pretreatment with an indeno-indole compound and ascorbate monitored in vivo by 31P magnetic resonance spectroscopy.
OBJECTIVE
To investigate whether combined pretreatment with lipid- and water-soluble antioxidants gave better restoration of energy phosphates after ischaemia-reperfusion of rabbit kidneys than single pretreatment with a lipid-soluble antioxidant.


METHODS
Thirteen New Zealand white rabbits were used for the study. Changes in energy phosphates were measured in vivo using volume-selective 31P magnetic resonance spectroscopy. The indeno-indole compound H290/51 was chosen as a lipid-soluble antioxidant and ascorbate as a water-soluble antioxidant.


RESULTS
The combined pretreatment led to significantly better restoration of the beta-adenosine triphosphate:inorganic phosphate ratio after 60 min of ischaemia and 120 min of reperfusion compared with the single pretreatment. Analyses of blood pressure and blood gas changes showed that the beneficial effect of combined pretreatment was not caused by a better general condition of the animals in that group but by a direct effect on the kidneys.


CONCLUSIONS
Combined pretreatment with lipid- and water-soluble antioxidants leads to better restoration of energy phosphates in rabbit kidneys subjected to ischaemia-reperfusion compared with single pretreatment with a lipid-soluble antioxidant.
Jonsson
Olof
O
Department of Urology, Lundberg and Wallenberg Laboratory, Sahlgrenska University Hospital, Göteborg, Sweden. olof.jonsson@vgregion.se
Lindgård
Ann
A
Fae
Anita
A
Gondalia
Jagdish
J
Aneman
Anders
A
Soussi
Bassam
B
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D007211:Indoles / Q000494:pharmacology*; D007511:Ischemia / Q000175:diagnosis / Q000188:drug therapy*; D007674:Kidney Diseases / Q000175:diagnosis / Q000188:drug therapy*; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008991:Monitoring, Physiologic; D010759:Phosphorus Isotopes; D011336:Probability; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D012995:Solubility
D000975:Antioxidants; C088233:H290-51; D007211:Indoles; D010759:Phosphorus Isotopes; D001205:Ascorbic Acid