title: [Study on the inhibition of alpha-glucosidase by soyasaponins].
abstract: OBJECTIVE
  To study the inhibitory effects of soyasaponins on alpha-glucosidase (EC3.2.1.20).
  
  
  METHODS
  Soyasaponins were isolated by ODS column chromatography and high-performance liquid chromatography (HPLC) from hypocotyls of soybean. The inhibitory activities of each component of soyasaponins against alpha-glucosidase were tested by colorimetric method.
  
  
  RESULTS
  Soyasaponins showed potent inhibitory activities against alpha-glucosidase. Group B, group E and DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins showed stronger potency, which were non-competitive inhibitors of alpha-glucosidase with IC50 values of 10-40 mumol/L. While group A saponins showed a little lower potency with IC50 values of about 2 mmol/L.
  
  
  CONCLUSIONS
  The results suggest soyasaponins, which exhibit inhibitory effects on alpha-glucosidase, seem physiologically useful for suppressing postprandial hyperglycemia in patients with diabetes mellitus.
authors.affiliation: Yanbian University, Yanji 133000.
authors.forename: Jishu
authors.initials: J
authors.lastname: Quan
authors.forename: Xuezhe
authors.initials: X
authors.lastname: Yin
authors.forename: Ming
authors.initials: M
authors.lastname: Jin
authors.forename: Minghua
authors.initials: M
authors.lastname: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D012503:Saponins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013662:Tea / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D012503:Saponins; D013662:Tea