Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon.
Infection of hepatitis B virus (HBV) continues to be a significant health problem. alpha interferon (IFN-alpha) and gamma interferon (IFN-gamma) have been proven to be effective in inhibiting HBV replication. To study the global effect of HBV persistent existence on IFN induced cellular gene expression, cDNA microarrays dotted with 14 112 human genes were used to examine the transcriptional changes between an HBV DNA transfected cell line (HepG2.2.15) and its parental cell line (HepG2) after the treatment of IFN-alpha or IFN-gamma for 6 h. The results showed that many genes related to cell cycle, proliferation, apoptosis and new ESTs were regulated by IFN-alpha and/or IFN-gamma. Many genes involved in kinase and signal transduction, transcription regulation, antigen presentation and processing were differentially regulated between these two cell lines post IFN-alpha or IFN-gamma treatment. Interestingly, several IFN-differentially regulated genes, such as MyD88 and Diubiquitin, were found to inhibit HBV gene expression, and MyD88 was proved to inhibit HBV replication. Taken together, our results revealed the global effects of HBV persistent existence on IFN-induced cellular gene expression. The novel antiviral genes identified by microarray could be potentially developed as new anti-HBV drugs or for novel therapies.
Xiong
Wei
W
Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Wang
Xun
X
Liu
Xiao-Ying
XY
Xiang
Li
L
Zheng
Ling-Jie
LJ
Liu
Jiang-Xia
JX
Yuan
Zheng-Hong
ZH
D045744:Cell Line, Tumor / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D015203:Reproducibility of Results; D014162:Transfection
D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D016898:Interferon-alpha; D007371:Interferon-gamma; D007372:Interferons