Kinesin walks hand-over-hand.
Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 +/- 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps.
Yildiz
Ahmet
A
Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, IL 61801, USA.
Tomishige
Michio
M
Vale
Ronald D
RD
Selvin
Paul R
PR
D000255:Adenosine Triphosphate; D002232:Carbocyanines; D019281:Dimerization; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011487:Protein Conformation
D002232:Carbocyanines; D005456:Fluorescent Dyes; D020409:Molecular Motor Proteins; C089187:cyanine dye 3; D000255:Adenosine Triphosphate; D016547:Kinesins