Characterization of a new mRNA species from the human histamine N-methyltransferase gene.
Histamine N-methyltransferase (HNMT), a cytosolic histamine-metabolizing enzyme, is the only known product of the 50-kb human HNMT. Here, a detailed investigation of HNMT products revealed the existence of a new brain mRNA product of HNMT. This species, named HNMT-Short (HNMT-S), encodes a 126-amino-acid protein. Northern blot analysis detected HNMT-S mRNA (1.0 kb) in placenta, but not in several other human tissues. In addition, unlike the known HNMT cDNA, HNMT-S cDNA did not result in histamine-methylating activity after transfection into COS-7 cells. These studies show that HNMT-S is a new mRNA species and putative protein product from HNMT. The physiological role of HNMT-S remains to be investigated.
Barnes
William G
WG
Center for Neuropharmacology and Neuroscience, Albany Medical College MC-136, Albany, NY 12208, USA.
Grinde
Ellinor
E
Crawford
Dana R
DR
Herrick-Davis
Katharine
K
Hough
Lindsay B
LB
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006632:Histamine / Q000378:metabolism; D006637:Histamine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D007527:Isoenzymes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D006632:Histamine; D006637:Histamine N-Methyltransferase