The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas.
By positional cloning, we identified two breakpoint-spanning genes in a familial clear cell renal cell carcinoma (CCRCC)-associated t(1;3)(q32.1;q13.3): LSAMP and NORE1 (RASSF1 homolog). Both genes are downregulated in 9 of 9 RCC cell lines. While the NORE1A promoter predominantly presents partial methylation in 6 of the cell lines and 17/53 (32%) primary tumors, the LSAMP promoter is completely methylated in 5 of 9 cell lines and in 14/53 (26%) sporadic and 4 familial CCRCCs. Expression of LSAMP and NORE1A proteins in CCRCC cell lines inhibited cell proliferation. These characteristics indicate that LSAMP and NORE1A may represent new candidate tumor suppressors for CCRCC.
Chen
Jindong
J
Lab of Cancer Genetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Lui
Weng-Onn
WO
Vos
Michele D
MD
Clark
Geoffrey J
GJ
Takahashi
Masayuki
M
Schoumans
Jacqueline
J
Khoo
Sok Kean
SK
Petillo
David
D
Lavery
Todd
T
Sugimura
Jun
J
Astuti
Dewi
D
Zhang
Chun
C
Kagawa
Susumu
S
Maher
Eamonn R
ER
Larsson
Catharina
C
Alberts
Arthur S
AS
Kanayama
Hiro-omi
HO
Teh
Bin Tean
BT
D048868:Adaptor Proteins, Signal Transducing; D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019175:DNA Methylation; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; C454042:RASSF5 protein, human; C095362:limbic system-associated membrane protein; D020559:Monomeric GTP-Binding Proteins