Sequencing of Neisseria meningitidis penA gene: the key to success in defining penicillin G breakpoints.
Testing of susceptibility to penicillin G by E-test and sequencing of an internal fragment of the penA gene were done for 43 meningococcal strains. Those strains for which the MIC was >/=0.094 micro g/ml showed mosaic alleles, so 0.094 micro g/ml is suggested as the penicillin G intermediate breakpoint when E-test is used.
Arreaza
Luisa
L
Reference Laboratory for Meningococci, National Center of Microbiology, Institute of Health Carlos III, Madrid, Spain.
Salcedo
Celia
C
Alcalá
Belén
B
Uría
María José
MJ
Abad
Raquel
R
Enríquez
Rocío
R
Vázquez
Julio A
JA
D000483:Alleles; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000187:drug effects / Q000235:genetics*; D010400:Penicillin G / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D011110:Polymorphism, Genetic / Q000235:genetics
D000900:Anti-Bacterial Agents; D010406:Penicillins; D000667:Ampicillin; D010400:Penicillin G