Capsid-dependent and -independent postentry restriction of primate lentivirus tropism in rodent cells.
Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential sensitivity to infection by vesicular stomatitis virus G-pseudotyped lentiviruses and that differences between human immunodeficiency virus type 1 and simian immunodeficiency virus (SIVmac) infectivity are sometimes, but not always, governed by determinants in capsid-p2. In at least one case, resistance to SIVmac infection could be eliminated by saturation of target cells with noninfectious SIVmac particles. However, cross-saturation experiments and analysis of Fv1-null cells engineered to express natural or artificial Fv1 proteins revealed that lentivirus restriction in mouse cells is independent of Fv1. Overall, these findings indicate that novel restriction factors in rodents can modulate sensitivity to specific primate lentiviruses.
Hatziioannou
Theodora
T
Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York 10021, USA.
Cowan
Simone
S
Bieniasz
Paul D
PD
D000818:Animals; D002213:Capsid / Q000378:metabolism*; D002460:Cell Line; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology; D015498:HIV-2 / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D017958:Lentiviruses, Primate / Q000472:pathogenicity* / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D011506:Proteins / Q000378:metabolism; D012377:Rodentia / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
C103189:Fv1 protein, mouse; C028391:G protein, vesicular stomatitis virus; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D011506:Proteins; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins