title: A study on the fabrication of chemically modified bacteriorhodopsin film and its photochemical properties.
abstract: Chemically modified bacteriorhodopsin (BR) films embedded in gelatin matrix were fabricated. It was found that chemically modified BR/gelatin film remained the original conformation of BR and possessed homogeneity. Then, the photochemical conversion from all-trans O state to 9-cis P state in the chemically modified BR film/gelatin was investigated. For comparison purpose, the same conversion in the wild-type BR (BRwt) film was also presented here. To our know, it is the first report to show that the absorption changes of the P state in the chemically modified BR film were larger than in the BRwt film upon illumination with red light at the low actinic power density.
authors.affiliation: Institute of Chemistry, Center for Molecular Science, Key Laboratory of Colloid and Interface Sciences, The Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Zhang
authors.forename: Baofang
authors.initials: B
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001436:Bacteriorhodopsins / Q000138:chemical synthesis* / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry; D007477:Ions; D011487:Protein Conformation; D015202:Protein Engineering; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D007477:Ions; D001436:Bacteriorhodopsins; D005780:Gelatin