[Effect of nitrite-anions on Ca2+ transport into the sarcolemma of myometrium].
The influence of nitrite-anions physiological concentration on Ca2+ input into vesicles was investigated when using the "outside-out" vesicles of myometrial plasmalemma and 45Ca2+. It was established that nitrite-anions increased Ca(2+)-permeability of plasmalemma and increased the affinity of cation-transport system. The effects are probably connected with reversible modification of glutamate residues that bound and transported Ca2+ within the membrane. These findings showed that nitrite-anions are competitive activators of the passive calcium transport. On the other hand the decrease of Ca2+ affinity for the transport system under transmembrane proton scattering by the membrane, by rapid dissipation of transmembrane delta pH. It may be possible that the dissipation of transmembrane proton gradient changed the conformation of calcium transport system that calls the difference of kinetic mechanism of NO2- action in case of delta pH = 0 and delta pH = 1.5 on vesicle membranes.
Danylovych
Iu V
IuV
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
D000818:Animals; D000838:Anions; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D017136:Ion Transport; D007700:Kinetics; D009215:Myometrium / Q000378:metabolism*; D009573:Nitrites / Q000494:pharmacology*; D012508:Sarcolemma / Q000378:metabolism*; D013552:Swine
D000838:Anions; D002132:Calcium Radioisotopes; D009573:Nitrites; C041349:potassium nitrite; D002118:Calcium