[The primary study on the anti-HBV effect of whole recombinant yeast].
OBJECTIVE
Based on the immunologic character of Pichia pastoris yeast, a new therapeutic vaccine, whole recombinant yeast, was used to explore a new way to activate cell-mediated anti-viral immunity.


METHODS
The recombinant plasmids, pPIC9K/S and PIC9K/hsp(1-370)-S, were constructed by inserting the gene encoding HBsAg, HSP70 (1-370) -HBsAg into vector pPIC9K and then the recombinants were transfected into Pichia pastoris yeast,GS115, respectively. Then that recombinant yeast immunized BALB/C mice were detected for humoral and cellular immunity to HBsAg.


RESULTS
Recombinant yeast successfully activated the humoral immunity to HBsAg in mice, but failed to activate the cellular immunity.


CONCLUSIONS
The whole recombinant yeast can be used as vaccine, but need further study for optimal way of immunization.
Zeng
Yan
Y
The Institute for Viral Hepatitis, Chongqing University of Medical Science, Chongqing 400010, China.
Cai
Da-chuan
DC
Peng
Ming-li
ML
Ren
Hong
H
D000818:Animals; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000235:genetics*; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D018840:HSP70 Heat-Shock Proteins; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D014614:Vaccines, Synthetic