title: [Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis].
abstract: OBJECTIVE
  To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.
  
  
  METHODS
  Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.
  
  
  RESULTS
  67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.
  
  
  CONCLUSIONS
  Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.
authors.affiliation: General Hospital of Beijing Coal Mineral Bureau, Beijing 102308, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Hongmin
authors.initials: H
authors.lastname: Li
authors.forename: Dongjin
authors.initials: D
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Bai
authors.initials: B
authors.lastname: Feng
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Guoyang
authors.initials: G
authors.lastname: Wang
authors.forename: Sumei
authors.initials: S
authors.lastname: Li
authors.forename: Huixin
authors.initials: H
authors.lastname: Han
mesh_terms: D003031:Coal; D024881:Drug Resistance, Bacterial; D006801:Humans; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012830:Silicotuberculosis / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology
chemical_list: D003031:Coal