title: Establishment of a two-dimensional electrophoresis map for Neospora caninum tachyzoites by proteomics.
abstract: Expressed proteins and antigens from Neospora caninum tachyzoites were studied by two-dimensional gel electrophoresis and immunoblot analysis combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Thirty-one spots corresponding to 20 different proteins were identified from N. caninum tachyzoites by peptide mass fingerprinting. Six proteins were identified from a N. caninum database (NTPase, 14-3-3 protein homologue, NcMIC1, NCDG1, NcGRA1 and NcGRA2), and 11 proteins were identified in closely related species using the T. gondii database (HSP70, HSP60, pyruvate kinase, tubulin alpha- and beta-chain, putative protein disulfide isomerase, enolase, actin, fructose-1,6-bisphosphatase, lactate dehydrogenase and glyceradehyde-3-phosphate dehydrogenase). One hundred and two antigen spots were observed using pH 4-7 IPG strips on immunoblot profiles. Among them, 17 spots corresponding to 11 antigenic proteins were identified from a N. caninum protein map. This study involved the construction of in-depth protein maps for N. caninum tachyzoites, which will be of value for studies of its pathogenesis, drug and vaccine development, and phylogenetic studies.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, 900 Gazwa-Dong, Jinju, Gyeong-nam 660-701, South Korea.
authors.forename: Eung Goo
authors.initials: EG
authors.lastname: Lee
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Yong Seung
authors.initials: YS
authors.lastname: Shin
authors.forename: Gee Wook
authors.initials: GW
authors.lastname: Shin
authors.forename: Myung Deuk
authors.initials: MD
authors.lastname: Suh
authors.forename: Dae Yong
authors.initials: DY
authors.lastname: Kim
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Gon Sup
authors.initials: GS
authors.lastname: Kim
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D007525:Isoelectric Focusing; D018688:Neospora / Q000737:chemistry* / Q000276:immunology; D020543:Proteome / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000913:Antibodies, Protozoan; D000954:Antigens, Surface; D020543:Proteome; D015800:Protozoan Proteins