title: Moenomycin-resistance is associated with vancomycin-intermediate susceptibility in Staphylococcus aureus.
abstract: We have previously isolated a vancomycin-intermediate susceptibility mutant from methicillinresistant Staphylococcus aureus(MRSA) strain COL, and demonstrated the increased glycan-chain length and the decreased moenomycin-susceptibility. To further investigate the relationship between the resistance to vancomycin and to moenomycin, we isolated moenomycin-resistant mutants (4-16 fold higher compared to the parent) from 5 MRSA and 2 methicillin-sensitive S. aureus(MSSA) strains. The MRSA mutants showed a decreased susceptibility to vancomycin (2-4 fold), teicoplanin (2-4 fold) and an increased susceptibility to methicillin (2-8 fold). MSSA strains also showed similar results with those of MRSA strains except that there was no alteration of methicillin susceptibility. Among the mutants, three mutants including two MRSA mutants and one MSSA mutant were analyzed by electron microscopy, and they showed thickened cell walls compared to those of the parents. The glycan-chain length of the peptidoglycan of the mutant was shown to be slightly longer than that of the parent, but the muropeptide profile was very similar. The expression levels of all PBPs were similar to those of the parent. Furthermore, the nucleotide sequences of sgtA, sgtB and pbp2 in the mutant were identical to those of the parent. These results indicate that the moenomycin-resistance is closely associated with vancomycin-intermediate susceptibility in S. aureus.
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi, Hiroshima, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nishi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Komatsuzawa
authors.forename: Sakuo
authors.initials: S
authors.lastname: Yamada
authors.forename: Tamaki
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohara
authors.forename: Kouji
authors.initials: K
authors.lastname: Ohta
authors.forename: Masaru
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Takenori
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000032:analysis / Q000235:genetics; D008248:Lysostaphin / Q000494:pharmacology; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis / Q000235:genetics; D009154:Mutation; D009844:Oligosaccharides / Q000494:pharmacology*; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000737:chemistry; D010458:Peptidyl Transferases / Q000032:analysis / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D017334:Teicoplanin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D009844:Oligosaccharides; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D011134:Polysaccharides; D017334:Teicoplanin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008248:Lysostaphin; C419802:moenomycin; D008712:Methicillin