Chronic allergy to dietary ovalbumin induces lymphocyte migration to rat small intestinal mucosa that is inhibited by MAdCAM-1.
Few models have described a chronic food allergy with morphological changes in the intestinal mucosa. Here we established an ovalbumin (OVA)-induced, cell-mediated, allergic rat model and examined lymphocyte migration in the gut. Brown Norway rats were intraperitoneally sensitized to OVA and then given 10 mg OVA/day by gastric intubation for 6 wk. Lymphocyte subsets and adhesion molecules were examined immunohistochemically, and the migration of T lymphocytes to microvessels of Peyer's patches and villus mucosa was observed by using an intravital microscope. Serum OVA-specific IgG and IgE levels were increased in animals repeatedly exposed to OVA. Significant villus atrophy and increased crypt depth was accompanied by increased infiltration of T lymphocytes in the small intestinal mucosa of the group given OVA. Expression of rat mast cell protease II and of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was also increased in these groups. The administration of anti-MAdCAM-1 antibody significantly attenuated the OVA-induced changes in the mucosal architecture and in CD4 T lymphocyte infiltration. Intravital observation demonstrated that in rats with a chronic allergy, T lymphocytes significantly accumulated in villus microvessels as well as in Peyer's patches via a MAdCAM-1-dependent process. Our model of chronic food allergy revealed that lymphocyte migration was increased with MAdCAM-1 upregulation.
Ogawa
Toshiko
T
School of Medicine, Keio Univ., 35 Shinanomichi, Shijuku-Ku, Tokyo 160-8582, Japan.
Miura
Soichiro
S
Tsuzuki
Yoshikazu
Y
Ogino
Takashi
T
Teramoto
Ken
K
Inamura
Toshiaki
T
Watanabe
Chikako
C
Hokari
Ryota
R
Nagata
Hiroshi
H
Ishii
Hiromasa
H
D000818:Animals; D000906:Antibodies / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement; D002908:Chronic Disease; D004032:Diet; D005512:Food Hypersensitivity / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000276:immunology; D007136:Immunoglobulins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000098:blood supply / Q000503:physiopathology; D007421:Intestine, Small / Q000098:blood supply / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D016131:Lymphocyte Subsets / Q000473:pathology; D008214:Lymphocytes; D008297:Male; D008871:Microvilli; D009088:Mucoproteins / Q000378:metabolism*; D010047:Ovalbumin / Q000008:administration & dosage / Q000009:adverse effects*; D010581:Peyer's Patches / Q000098:blood supply; D051381:Rats; D011914:Rats, Inbred BN; D012697:Serine Endopeptidases / Q000378:metabolism; D014699:Venules / Q000503:physiopathology
D000906:Antibodies; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C504268:Madcam1 protein, rat; D009088:Mucoproteins; D010047:Ovalbumin; D012697:Serine Endopeptidases; C057818:cytotoxic T lymphocyte-specific serine protease