title: [Detection and significance of succinate dehydrogenase of sperm mitochondria].
abstract: OBJECTIVE
  To detect succinate dehydrogenase (SDH) of sperm mitochondria by an improved method, and to evaluate the relationship between SDH and the motility and viability of sperm.
  
  
  METHODS
  In 46 fertile and infertile men (aged from 25 to 36), the motility, viability and mitochondrial SDH of sperm were detected by computer-assisted semen analysis system, dead/live sperm molecular fluorescent probe and an improved cytochemical method. Then, the correlation between the motility and viability of sperm and the positive percentage of sperm mitochondrial SDH were analyzed.
  
  
  RESULTS
  In the 46 fertile and infertile men, the motility and viability of sperm were (67.33 +/- 7.37)% and (79.78 +/- 7.65)%, and the positive percentage of sperm mitochondrial SDH was (74.74 +/- 8.29)%. The motility and viability of sperm and positive percentage of sperm mitochondrial SDH had significant correlation (r = 0.901, P < 0.01; r = 0.876, P < 0.01; r = 0.917, P < 0.01).
  
  
  CONCLUSIONS
  Detection of sperm mitochondrial SDH has significance in evaluation of sperm mitochondrial function and may serve as an assisted marker of sperm viability.
authors.affiliation: Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Xue
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Shang
authors.forename: Jian
authors.initials: J
authors.lastname: Fu
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Chen
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Shi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002470:Cell Survival; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007248:Infertility, Male / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D013385:Succinate Dehydrogenase