Magnetic resonance histology: in situ single cell imaging of receptor cells in an invertebrate (Lolliguncula brevis, Cephalopoda) sense organ.
Utilizing contrast-enhanced MR histology, individual cell bodies were identified in situ and compared one-to-one with conventional histology. The squid Lolliguncula brevis served as a model where the receptor cells of the proprioceptive neck receptor organ were labeled with paramagnetic cobalt(II) ions by conventional cobalt iontophoresis. Stimulated echo images were obtained using a 9.4 T magnet and followed by conventional histologic treatment and light microscopy. Images obtained from both these techniques match well and validate MR histology.
Gozansky
Elliott K
EK
Department of Radiology, The University of Texas Medical Branch, Galveston, TX, USA. elliot.gozansky@utmb.edu
Ezell
Edward L
EL
Budelmann
Bernd U
BU
Quast
Michael J
MJ
D000818:Animals; D003035:Cobalt; D049832:Decapodiformes; D005260:Female; D007202:Indicators and Reagents; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008853:Microscopy / Q000379:methods; D023421:Models, Animal; D011434:Proprioception / Q000502:physiology; D012679:Sense Organs / Q000166:cytology*; D011984:Sensory Receptor Cells / Q000166:cytology
D007202:Indicators and Reagents; D003035:Cobalt