title: AthaMap: an online resource for in silico transcription factor binding sites in the Arabidopsis thaliana genome.
abstract: Gene expression is controlled mainly by the binding of transcription factors to regulatory sequences. To generate a genomic map for regulatory sequences, the Arabidopsis thaliana genome was screened for putative transcription factor binding sites. Using publicly available data from the TRANSFAC database and from publications, alignment matrices for 23 transcription factors of 13 different factor families were used with the pattern search program Patser to determine the genomic positions of more than 2.4 x 10(6) putative binding sites. Due to the dense clustering of genes and the observation that regulatory sequences are not restricted to upstream regions, the prediction of binding sites was performed for the whole genome. The genomic positions and the underlying data were imported into the newly developed AthaMap database. This data can be accessed by positional information or the Arabidopsis Genome Initiative identification number. Putative binding sites are displayed in the defined region. Data on the matrices used and on the thresholds applied in these screens are given in the database. Considering the high density of sites it will be a valuable resource for generating models on gene expression regulation. The data are available at http://www.athamap.de.
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany.
authors.forename: Nils Ole
authors.initials: NO
authors.lastname: Steffens
authors.forename: Claudia
authors.initials: C
authors.lastname: Galuschka
authors.forename: Martin
authors.initials: M
authors.lastname: Schindler
authors.forename: Lorenz
authors.initials: L
authors.lastname: Bülow
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hehl
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology; D030541:Databases, Genetic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D023281:Genomics; D016247:Information Storage and Retrieval; D020407:Internet; D008969:Molecular Sequence Data; D020218:Response Elements / Q000235:genetics*; D016415:Sequence Alignment; D012984:Software; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors