Binding of antibodies to human immunodeficiency virus type 1 (HIV-1)-infected lymphocytes elicited by vaccines and by natural infection.
Binding of antibodies to oligomeric envelope glycoprotein of R5-tropic primary isolates of human immunodeficiency virus type 1 (HIV-1) was studied by flow cytometry using sera from HIV-1 vaccine recipients and clade B and C HIV-1-infected patients, and monoclonal and polyclonal antibodies to neutralizing epitopes of HIV-1. Vaccine recipients received recombinant canarypox virus vaccine expressing HIV-1 gene products, and SF-2 recombinant gp120 subunit vaccine. Anti-gp120 neutralizing antibodies including human monoclonal antibody 2G12 and goat polyclonal anti-serum to V3 loop peptide [peptide T1-SP10MN(A)] bound to HIV-1-infected cells. Sera from vaccine recipients bound to HIV-1-infected cells, but at levels lower than did infected patient sera.
Gorse
Geoffrey J
GJ
St. Louis Department of Veterans Affairs Medical Center, St. Louis University, St. Louis, MO 63110, USA. gorsegj@slu.edu
Patel
Gira B
GB
Arbuckle
J Alan
JA
Belshe
Robert B
RB
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D028121:Canarypox virus / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015686:Gene Products, env / Q000378:metabolism; D006041:Goats / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology
D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015686:Gene Products, env; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic