Augmentation by 2-mercaptoethanol of in vitro anti-TNP antibody production induced by butyrate plus IL-2 in murine splenic B cells.
We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.
Gohda
Eiichi
E
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama, Japan.
Okamura
Takayuki
T
Aoyama
Eriko
E
Yamamoto
Itaru
I
D000107:Acetylation; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D002087:Butyrates / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D005232:Fatty Acids, Volatile / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000008:administration & dosage / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008623:Mercaptoethanol / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013438:Sulfhydryl Compounds / Q000008:administration & dosage / Q000494:pharmacology
D002087:Butyrates; D005232:Fatty Acids, Volatile; D006657:Histones; D007376:Interleukin-2; D008070:Lipopolysaccharides; D013438:Sulfhydryl Compounds; C048761:trinitrophenyl-lipopolysaccharide; D008623:Mercaptoethanol