[Effect of fluoroaluminate on the ATP-hydrolysing and Ca2+-transporting activity of the purified Ca2+, Mg2+-ATPase of myometrium cell plasma membranes].
Fluoroaluminate, known modulator of G-proteins, inhibits ATP-hydrolase activity of purified solubilized Ca2+, Mg(2+)-ATPase from myometrium cell plasma membranes and Ca(2+)-transporting activity of this enzyme reconstituted into azolectin liposomes: 10 mM NaF plus 10 microM AlCl3 inhibited the primary activity by 95% and--by 81%. Inhibition of purified both solubilized and reconstituted Ca2+, Mg(2+)-ATPases by fluoroaluminate evidences for the possibility of direct interaction AlF4- with this enzyme without involvement of G-protein. The sensitivity to fluoroaluminate of sarcolemmal Ca2+, Mg(2+)-ATPase from myometrium is similar to that of Ca2+, Mg(2+)-ATPase from stomach smooth muscle.
Slinchenko
N M
NM
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. slinchenko@biochem.kiev.ua
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005461:Fluorine / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009215:Myometrium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013270:Stomach / Q000187:drug effects / Q000201:enzymology
C053629:fluoroaluminum; D005461:Fluorine; D000255:Adenosine Triphosphate; D000535:Aluminum; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium