title: Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase.
abstract: A truncated version of the spinosyn polyketide synthase comprising the loading module and the first four extension modules fused to the erythromycin thioesterase domain was expressed in Saccharopolyspora erythraea. A novel pentaketide lactone product was isolated, identifying cryptic steps of spinosyn biosynthesis and indicating the potential of this approach for the biosynthetic engineering of spinosyn analogues. A pathway for the formation of the tetracyclic spinosyn aglycone is proposed.
authors.affiliation: Biotica Technology Ltd, Building 5, Chesterford Research Park, Little Chesterford, Nr Saffron Walden, Essex, UK CB10 1XL. Christine.Martin@biotica.co.uk
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Martin
authors.forename: Máire C
authors.initials: MC
authors.lastname: Timoney
authors.forename: Rose M
authors.initials: RM
authors.lastname: Sheridan
authors.forename: Steven G
authors.initials: SG
authors.lastname: Kendrew
authors.forename: Barrie
authors.initials: B
authors.lastname: Wilkinson
authors.forename: James C
authors.initials: JC
authors.lastname: Staunton
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
mesh_terms: D000595:Amino Acid Sequence; D018942:Macrolides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D016930:Saccharopolyspora / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D018942:Macrolides; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C114883:spinosyn A; C114884:spinosyn D