Determination of new derivatives of genistein in culture media by liquid chromatography.
Methods for determination of genistein and its four new analogues in culture media have been developed to support studies on their potential anticancer activities. The investigated compounds were extracted from the media using liquid-liquid extraction with appropriate solvent. After evaporation of organic solvents each of the dry extracts was reconstituted in appropriate mobile phase. Reversed-phase HPLC was applied to quantitative determining of tested compounds. The methods are specific, sensitive and technically simple. They were used to evaluate concentration level of investigated compounds in experiments with human promyelocytic leukemia cells (HL-60 cell line).
Ksycińska
Hanna
H
Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793, Warsaw, Poland. hanka@ifarm.waw.pl
Sobik
Barbara
B
Popiołkiewicz
Joanna
J
Polkowski
Krzysztof
K
Krzeczyński
Piotr
P
Ramza
Jan
J
Pucko
Wieslaw
W
Grynkiewicz
Grzegorz
G
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D019833:Genistein / Q000032:analysis*; D018922:HL-60 Cells; D006801:Humans; D012680:Sensitivity and Specificity; D012997:Solvents
D003470:Culture Media; D012997:Solvents; D019833:Genistein