Polymorphism of the MICA gene and risk for oral submucous fibrosis.
BACKGROUND
Oral submucous fibrosis (OSF), an insidious, pre-cancerous, chronic disease that may affect the entire oral cavity and sometimes extend to the pharynx has been reported to be associated with immune function. The major histocompatibility complex (MHC) class I chain-related gene A (MICA) is expressed by keratinocytes and other epithelial cells, and its encoded protein interacts with gamma/delta T-cells localized in the submucosa. The MICA gene has a triplet repeat (GCT) polymorphism in the transmembrane domain resulting in five distinct allelic patterns.


METHODS
We analyzed MICA polymorphism in 80 OSF patients and 351 randomly selected unrelated controls by using the ABI Prism 377-18 DNA sequencer (Applied Biosystems) to analyze the sample DNA PCR products. The number of microsatellite repeats was estimated with Genescan 672 software (Applied Biosystems) with a standard size marker of GS-350 TAMRA.


RESULTS
The phenotype frequency of allele A6 of MICA in subjects with OSF was significantly higher than that in controls (OR = 3.48; 95% CI = 1.8-6.71; P = 0.0002), as was the frequency of the allele (OR = 2.65; 95% CI = 1.44-4.86; P = 0.001).


CONCLUSIONS
The results suggest that allele A6 in MICA might confer a risk for OSF.
Liu
Chung-Ji
CJ
Departments of Oral and maxillofacial surgery, Taipei Mackay Memorial Hospital, School of Dentistry, National Yang-Ming University, Taipei, Taiwan. cjliu@ms2.mmh.org.tw
Lee
Yann-Jinn
YJ
Chang
Kuo-Wei
KW
Shih
Yi-Ning
YN
Liu
Hsin-Fu
HF
Dang
Ching-Wen
CW
D000328:Adult; D000368:Aged; D000483:Alleles; D016001:Confidence Intervals; D003596:Cytosine; D005260:Female; D005787:Gene Frequency; D006147:Guanine; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D016017:Odds Ratio; D009914:Oral Submucous Fibrosis / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D012737:Sex Factors; D013941:Thymine
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D008565:Membrane Proteins; D006147:Guanine; D003596:Cytosine; D013941:Thymine