[The use of the [13C]/[12C] ratio for the assay of the microbial oxidation of hydrocarbons].
The study deals with a comparative analysis of the relative abundances of the carbon isotopes 12C and 13C in the metabolites and biomass of the Burkholderia sp. BS3702 and Pseudomonas putida BS202-p strains capable of utilizing aliphatic (n-hexadecane) and aromatic (naphthalene) hydrocarbons as sources of carbon and energy. The isotope composition of the carbon dioxide, biomass, and exometabolites produced during the growth of Burkholderia sp. BS3702 on n-hexadecane (delta 13C = -44.6 +/- 0.2@1000) were characterized by the isotope effects delta 13CCO2 = -50.2 +/- 0.4@1000, delta 13Cbiom = -46.6 +/- 0.4@1000 and delta 13Cexo = -41.5 +/- 0.4@1000, respectively. The isotope composition of the carbon dioxide, biomass, and exometabolites produced during the growth of the same bacterial strain on naphthalene (delta 13C = -21 +/- 0.4@1000) were characterized by the isotope effects delta 13CCO2 = -24.1 +/- 0.4@1000, delta 13Cbiom = -19.2 +/- 0.4@1000 and delta 13Cexo = -19.1 +/- 0.4@1000, respectively. The possibility of using the isotope composition of metabolic carbon dioxide for the rapid monitoring of the microbial degradation of petroleum hydrocarbons in the enviroment is discussed.
Ziakun
A M
AM
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia. zyakun@ibpm.pushchino.ru
Kosheleva
I A
IA
Zakharchenko
V N
VN
Kudriavtseva
A I
AI
Peshenko
V A
VA
Filonov
A E
AE
Boronin
A M
AM
D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
D002247:Carbon Isotopes; D006838:Hydrocarbons