[Estimation of telomerase transcriptive activity in the umbilical cord and the mother's venous blood cells].
Stem cells possess the ability of the partition and differentiation into other cells, practically lifelong of the organism. The potential of the self-extraction of these cells practically unrestricted and results probably from the telomerase gene activity, especially subunit hTERT. To the analysis of 11 samples of the umbilical cord and mothers blood received immediately after the childbirth in which one compared the expression of telomerase genes hTERT, TP1, hTR. Four cases ascertained the expression subunit hTERT in the umbilical cord blood at the lack its transcripts in the venous blood of mothers. In one case we found 10-times higher concentration of subunit hTERT in the umbilical cord blood than in the venous blood of the mother. 6 cases did not demonstrate the expression of the hTERT nor in the umbilical cord blood nor in the venous blood of mothers. The transcriptive activity TP1 and hTR did not show differences among investigated groups. Received results put the question or the transcriptive activity subunit hTERT in the umbilical cord blood is a results of the presence of stem cells.
Stojko
Rafał
R
Katedry i Kliniki Połoznictwa i Ginekologii SlAM w Katowicach.
Głogowska
Joanna
J
Wilczok
Adam
A
Mazurek
Urszula
U
Witek
Andrzej
A
Wilczok
Tadeusz
T
D000328:Adult; D004268:DNA-Binding Proteins; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D011247:Pregnancy; D012313:RNA; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D019098:Telomerase / Q000097:blood / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase