title: A new human natural killer leukemia cell line, IMC-1. A complex chromosomal rearrangement defined by spectral karyotyping: functional and cytogenetic characterization.
abstract: A new human IL-2 dependent leukemic cell line with a natural killer (NK) cell phenotype, IMC-1, was established from an adult patient with aggressive NK cell leukemia. The IMC-1 cell line expresses the CD56, CD2, CD11a, CD38 and HLA-DR cell surface antigens, whereas the CD16 and CD8 antigens expressed on the primary leukemic blasts from which the cell line was derived were lost after 7 and 28 weeks of culture, respectively. The IMC-1 cell line displays functional NK cytotoxicity and lyses target cells in a non-MHC restricted, antibody-independent manner with equal or superior efficiency to freshly isolated NK cells. Cytogenetic analysis at presentation and after 55 weeks in culture revealed complex structural and numerical abnormalities, defined by classic G-banding and by spectral karyotyping (SKY). Three apparently intact copies of chromosome 8 occurred in the diagnostic bone marrow specimen; the cell line also contains three copies of chromosome 8 but each was structurally altered. The development and detailed characterization of this new NK leukemic cell line will facilitate biologic and functional studies of NK cells and chromosomal aberrations potentially important in leukemic transformation.
authors.affiliation: Department of Pathology, University of New Mexico School of Medicine, 900 Camino de Salud NE, Albuquerque, NM 87131-5636, USA.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Chen
authors.forename: Margaret
authors.initials: M
authors.lastname: Whalen
authors.forename: Arthur
authors.initials: A
authors.lastname: Bankhurst
authors.forename: Cordelia E
authors.initials: CE
authors.lastname: Sever
authors.forename: Rashmi
authors.initials: R
authors.lastname: Doshi
authors.forename: David
authors.initials: D
authors.lastname: Hardekopf
authors.forename: Karen
authors.initials: K
authors.lastname: Montgomery
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Willman
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D045744:Cell Line, Tumor / Q000737:chemistry / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics / Q000648:ultrastructure*; D003602:Cytotoxicity, Immunologic; D017809:Fatal Outcome; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007694:Killer Cells, Natural / Q000737:chemistry / Q000166:cytology*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology*; D014314:Trisomy
chemical_list: D000951:Antigens, Neoplasm