IL-1 beta-mediated neuropeptide and immediate early gene mRNA induction is defective in Lewis hypothalamic cell cultures.
We previously found that Lewis (LEW/N) hypothalamic cells respond to interleukin-1beta (IL-1beta) with reduced corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) peptide synthesis and secretion compared to Fischer (F344/N) cells. To investigate whether this peptide hyporesponsiveness in LEW/N cells is secondary to their deficient mRNA expression, temporal mRNA expression patterns of CRH, AVP, and several hypothalamic neuropeptides induced by IL-1beta in LEW/N and F344/N hypothalamic dissociated cell cultures were delineated by quantitative real-time polymerase chain reaction (RT-PCR). To investigate the molecular mechanisms underlying neuropeptide mRNA induction in cells of both strains, temporal mRNA expression patterns of immediate early genes (IEGs) and several signal transduction-associated molecules were also examined. We found that LEW/N hypothalamic cells were hyporesponsive to IL-1beta induction of neuropeptide and IEG mRNA, while LEW/N cells transcribed more IL-1 receptor and inducible nitric oxide synthase (iNOS) compared to F344N/N cells, suggesting that LEW/N and F344/N hypothalamic cells are differentially activated by IL-1beta.
Wei
Rongtai
R
Integrative Neural Immune Program, NIMH, NIH, 36 Convent Drive, Building 36, Room 1A23, Bethesda, MD 20892-4020, USA.
Sternberg
Esther M
EM
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D017781:Genes, Immediate-Early; D007031:Hypothalamus / Q000378:metabolism*; D007375:Interleukin-1 / Q000502:physiology*; D009479:Neuropeptides / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D013045:Species Specificity
D007375:Interleukin-1; D009479:Neuropeptides; D012333:RNA, Messenger