title: A diabetes-related epitope of GAD65: a major diabetes-related conformational epitope on GAD65.
abstract: The 65-kDa isoform of glutamic acid decarboxylase (GAD65) is a major autoantigen in type 1 diabetes, and most patients have serum antibodies reactive with conformational epitopes on the GAD65 molecule. The aims of this study were to prepare mutants of GAD65 to further localize the type 1 diabetes epitope in the region of the PEVKEK loop of GAD65 and to identify the particular amino acids within the epitope that are recognized by autoimmune diabetes sera.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Myers
authors.forename: Gustavo
authors.initials: G
authors.lastname: Fenalti
authors.forename: Robyn
authors.initials: R
authors.lastname: Gray
authors.forename: Marita
authors.initials: M
authors.lastname: Scealy
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Tong
authors.forename: Ossama
authors.initials: O
authors.lastname: El-Kabbani
authors.forename: Merrill J
authors.initials: MJ
authors.lastname: Rowley
mesh_terms: D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006801:Humans; D007527:Isoenzymes / Q000276:immunology*; D011487:Protein Conformation
chemical_list: D001323:Autoantibodies; D000939:Epitopes; D007527:Isoenzymes; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2