title: Epididymal sperm from the African buffalo (Syncerus caffer) can be frozen successfully with AndroMed and with Triladyl but the addition of bovine seminal plasma is detrimental.
abstract: Numerous diseases are carried and can be transmitted from the African buffalo (Syncerus caffer) to livestock. Buffaloes free of specific diseases (BFSD) are thus in demand amongst game farmers. Current BFSD derive from a small genetic pool and hence there is a special interest in bringing new genetic material into such herds. In this study epididymal sperm from 16 mature African buffalo bulls was frozen with Triladyl and AndroMed extender (Minitüb, Tiefenbach, Germany) with and without addition of bovine seminal plasma. Post-thaw motility, longevity and acrosomal integrity were compared. In all but one animal, post-thaw motility was higher, although not always significant, if sperm was frozen with Triladyl than with AndroMed. Seminal plasma was detrimental to the post-thaw motility. Neither semen extender nor seminal plasma had an influence on post-thaw acrosomal integrity. It can be concluded that bovine seminal plasma at a concentration of 10% is detrimental rather than beneficial for the post-thaw motility of African buffalo sperm. Even though being inferior AndroMed does, however, have the advantage that it is a defined semen extender and therefore clearly has a lower risk of contamination.
authors.affiliation: Section of Reproduction, University of Pretoria, Onderstepoort, South Africa. f.herold@gmx.at
authors.forename: F C
authors.initials: FC
authors.lastname: Herold
authors.forename: J E
authors.initials: JE
authors.lastname: Aurich
authors.forename: D
authors.initials: D
authors.lastname: Gerber
mesh_terms: D000818:Animals; D002020:Buffaloes; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D004822:Epididymis / Q000166:cytology*; D006358:Hot Temperature; D008297:Male; D012661:Semen / Q000502:physiology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents