Characterization of the bovine BCL2L1 gene and related pseudogenes.
We have amplified and characterized partial regions of exons 2 and 3 of the bovine BCL2L1 gene, one of the anti-apoptotic members of the B-cell lymphoma 2 gene family. Cloning and sequencing of the amplified products revealed the existence of several BCL2L1-related sequences, including the bovine BCL2L1 gene and various processed pseudogenes. The bovine BCL2L1 gene revealed two polymorphic nucleotide sequences that resulted in two protein variants, with amino acid replacements at positions 60 and 69. In addition, we report three bovine BCL2L1-related sequences (BCL2L1psi) that probably correspond to intronless processed pseudogenes. These BCL2L1psi pseudogene sequences have accumulated multiple substitutions, deletions and insertions that translated into stop codons or changed the open reading frame of the functional gene. We provide evidence suggesting that the retro-transposition event that originated these processed pseudogenes took place before the divergence of the Cervidae and Bovidae families.
Amills
M
M
Laboratory of Immunogenetics, University of Illinois at Urbana-Champaign, IL, USA. marcel.amills@uab.es
Bouzat
J L
JL
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002417:Cattle / Q000145:classification / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D005091:Exons; D005784:Gene Amplification; D006801:Humans; D010802:Phylogeny; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics*; D011817:Rabbits; D051020:bcl-X Protein
C494811:BCL2L1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein