title: Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics.
abstract: Reactive oxygen species (ROS) produced either endogenously or exogenously can attack lipid, protein and nucleic acid simultaneously in the living cells. In nuclear and mitochondrial DNA, 8-hydroxydeoxyguanosine (8-OHdG), an oxidized nucleoside of DNA, is the most frequently detected and studied DNA lesion. Upon DNA repair, 8-OHdG is excreted in the urine. Numerous evidences have indicated that urinary 8-OHdG not only is a biomarker of generalized, cellular oxidative stress but might also be a risk factor for cancer, atherosclerosis and diabetes. For example, elevated level of urinary 8-OHdG has been detected in patients with various cancers. In human atherosclerotic plaques, there were increased amounts of oxidatively modified DNA and 8-OHdG. Elevated urinary 8-OHdG and leukocyte DNA were also detected in diabetic patients with hyperglycemia, and the level of urinary 8-OHdG in diabetes correlated with the severity of diabetic nephropathy and retinopathy. We have discussed various methods for determining 8-OHdG in the tissue and urine, including HPLC with and without extraction, and ELISA. Using the ELISA we developed, we found that the normal range of urinary 8-OHdG for females was 43.9 +/- 42.1 ng/mg creatinine and 29.6 +/- 24.5 ng/mg creatinine for males, respectively. We found that the normal value between females and males is significantly different (p < 0.001).
authors.affiliation: Department of Pathology, University of Utah Health Science Center, Salt Lake City, UT, USA.
authors.forename: Lily L
authors.initials: LL
authors.lastname: Wu
authors.forename: Chiuan Chian
authors.initials: CC
authors.lastname: Chiou
authors.forename: Pi Yueh
authors.initials: PY
authors.lastname: Chang
authors.forename: James T
authors.initials: JT
authors.lastname: Wu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D001161:Arteriosclerosis / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism / Q000652:urine*; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism / Q000652:urine; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000652:urine; D018384:Oxidative Stress; D012307:Risk Factors
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine