Humanin improves impaired metabolic activity and prolongs survival of serum-deprived human lymphocytes.
Humanin (HN) has been reported to be an endogenous peptide that exerts highly selective neuroprotection against cell death induced by various types of Alzheimer's disease-related insults. We previously proposed the much broader cytoprotective potential of HN from the result that HN suppressed serum-deprivation-induced death of rat pheochromocytoma cells. In this study, we showed that HN also suppressed death of human lymphocytes cultured under serum-deprived condition. Further, we revealed, by assaying metabolic activity and survival rate, that HN was a potent factor capable of increasing the metabolic activity of individual serum-deprived lymphocytes. To our knowledge, there is no report described about a rescue factor that increases the metabolic activity of individual serum-deprived cells and prolongs their survival. This novel feature of HN may enable us to apply this peptide for the management of diseases involving poor metabolic activity, such as mitochondria-related disorders and brain ischemia.
Kariya
Shingo
S
Department of Neurology, Nara Medical University, Kashihara, Nara, Japan. neurology@yahoo.co.jp
Takahashi
Nobuyuki
N
Hirano
Makito
M
Ueno
Satoshi
S
D000328:Adult; D000544:Alzheimer Disease; D017209:Apoptosis; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D004272:DNA, Mitochondrial / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000502:physiology*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D004272:DNA, Mitochondrial; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; D011506:Proteins; C427211:humanin; D004247:DNA; D019833:Genistein