alpha3beta1 integrin-CD151, a component of the cadherin-catenin complex, regulates PTPmu expression and cell-cell adhesion.
The beta1 family of integrins has been primarily studied as a set of receptors for the extracellular matrix. In this paper, we define a novel role for alpha3beta1 integrin in association with the tetraspanin CD151 as a component of a cell-cell adhesion complex in epithelial cells that directly stimulates cadherin-mediated adhesion. The integrin-tetraspanin complex affects epithelial cell-cell adhesion at the level of gene expression both by regulating expression of PTPmu and by organizing a multimolecular complex containing PKCbetaII, RACK1, PTPmu, beta-catenin, and E-cadherin. These findings demonstrate how integrin-based signaling can regulate complex biological responses at multiple levels to determine cell morphology and behavior.
Chattopadhyay
Nibedita
N
Division of Nephrology, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Wang
Zemin
Z
Ashman
Leonie K
LK
Brady-Kalnay
Susann M
SM
Kreidberg
Jordan A
JA
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D019204:GTP-Binding Proteins; D039201:Integrin alpha3beta1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D046911:Macromolecular Substances; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D064546:Protein Kinase C beta; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D060185:Tetraspanin 24; D015534:Trans-Activators / Q000378:metabolism; D051176:beta Catenin
D015703:Antigens, CD; C554715:CD151 protein, human; C495267:CTNNB1 protein, mouse; D015820:Cadherins; C554716:Cd151 protein, mouse; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D039201:Integrin alpha3beta1; D007797:Laminin; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D060185:Tetraspanin 24; D015534:Trans-Activators; D051176:beta Catenin; C121346:laminin 1; D011493:Protein Kinase C; D064546:Protein Kinase C beta; C516862:PTPRN protein, human; D017027:Protein Tyrosine Phosphatases; C516863:Ptprn protein, mouse; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8; D019204:GTP-Binding Proteins