title: Salivary esterase activity and its association with the biodegradation of dental composites.
abstract: Pseudocholinesterase (PCE) and cholesterol esterase (CE) can hydrolyze bisphenylglycidyl dimethacrylate (bisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers. This study will test the hypothesis that enzyme activities showing CE and PCE character are found in human saliva at levels sufficient to hydrolyze ester-containing composites important to restorative denstistry. The study also seeks to ask if the active sites of CE and PCE with respect to composite could be inhibited. Photo-polymerized model composite resin was incubated in PCE and CE solutions, in the presence and absence of a specific esterase inhibitor, phenylmethylsulfonyl fluoride (PMSF). Incubation solutions were analyzed for resin degradation products by high-performance liquid chromatography (HPLC), UV spectroscopy, and mass spectrometry. Saliva was found to contain both hydrolase activities at levels that could degrade composite resins. PMSF inhibited the composite degradation, indicating a material hydrolysis mechanism similar to the enzymes' common function.
authors.affiliation: Restorative Discipline, aculty of Dentistry, University of Toronto, Toronto, ON, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Finer
authors.forename: J P
authors.initials: JP
authors.lastname: Santerre
mesh_terms: D000704:Analysis of Variance; D001673:Biodegradation, Environmental; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002091:Butyrylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003188:Composite Resins / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D012463:Saliva / Q000201:enzymology*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D002787:Sterol Esterase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D002800:Cholinesterase Inhibitors; D003188:Composite Resins; D003764:Dental Materials; D004791:Enzyme Inhibitors; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; D010664:Phenylmethylsulfonyl Fluoride; D002787:Sterol Esterase; D002091:Butyrylcholinesterase