[The effects of epidermal growth factor on the wound healing of deep partial thickness burn in rats].
OBJECTIVE
To observe the effects of recombinant human epidermal growth factor (rhEGF) on deep partial thickness burn wound healing.


METHODS
The rats were inflicted with deep partial thickness burn. The wounds were either treated with rhEGF, rhEGF with heparin or isotonic saline, respectively. Wound healing time was observed. Wound healing rate, water content and hydroxyproline (OHP) content and type I/III collagen ratio in the wound tissue were determined. Cellular DNA cycle analysis and histological examination were processed.


RESULTS
After the application of rhEGF, burn wound healing time was shortened by two days and wound content of OHP was increased (P < 0.05), with decreased collagen type I/III ratio (P < 0.05). As a result, granulation was enhanced and cellular DNA replication was accelerated (P < 0.05). Heparin addition could augment the effects of rhEGF, especially on wound healing time (2 days shorter) and granulation.


CONCLUSIONS
The wound healing time of the deep partial thickness burn could be accelerated markedly by topical application of rhEGF, but early application did not show obvious effects. The addition of heparin might further accelerate wound healing.
Zheng
Jun
J
Department of Burns and Plastic Surgery, Xiang Ya Hospital, Central South University, Changsha 410008, Hu Nan Province, P.R. China.
Huang
Xiao-Yuan
XY
Wei
Xing
X
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D006493:Heparin