Identification and sequence of seventy-nine new transcripts expressed in hemocytes of Ciona intestinalis, three of which may be involved in characteristic cell-cell communication.
Ascidian is a useful experimental animal for studying body planning principles and host defense mechanisms employed by the phylum chordata. Toward this goal, genome and cDNA/EST projects of Ciona intestinalis have been undertaken. Using cDNAs and ESTs derived from Ciona hemocytes, we identified 79 possible hemocyte-preferential transcripts and determined the cDNA sequence of each clone. The amino acid sequence of each encoded polypeptide was predicted as well. Among these cDNAs, we identified three transcripts that may be involved in characteristic cell-cell communication in Ciona. These transcripts encoded leucine-rich repeat-containing RP105-like, IL-17 receptor/similar expression to FGF-like, and ectodysplasin-like polypeptide of the tunmr necrosis factor famlly, and they are expressed abundantly in hemocytes.
Terajima
Daichi
D
Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.
Yamada
Shigeyuki
S
Uchino
Ryuji
R
Ikawa
Shuntaro
S
Ikeda
Makoto
M
Shida
Kazuhito
K
Arai
Yoichi
Y
Wang
Hong-Gang
HG
Satoh
Nori
N
Satake
Masanobu
M
D000818:Animals; D002450:Cell Communication; D002938:Ciona intestinalis / Q000235:genetics*; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D006434:Hemocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012333:RNA, Messenger