[Fluconazole resistance in Candida albicans assayed by PCR fingerprinting with M13 prime].
OBJECTIVE
To understand the molecular and genetic mechanism underlying fluconazole resistance in Candida albicans by PCR fingerprinting with M13 primer.


METHODS
Paper disc diffusion method was employed for assay of fluconazole resistance in 41 clinical isolates of Candida albicans, followed by PCR fingerprinting with M13 primer to study the gel patterns with cluster analysis using neighbor joining (NJ) method performed with RAPD200 software.


RESULTS
Of the 41 clinical isolates, 11 strains (26.8%) were fluconazole-sensitive, 8 (19.5%) fluconazole-dependent and 22 (53.7%) fluconazole-resistance. Two to twelve bands could be observed among these strains, and the gel patterns revealed by cluster analysis were associated with the reactions of the strains against fluconazole and the location of infection.


CONCLUSIONS
There is high prevalence of fluconazole resistance in clinical Candida albicans isolates, and PCR fingerprinting with M13 primer is convenient for assay of fluconazole resistance and molecular epidemiological study of Candida albicans.
Wang
Ying-Bin
YB
Department of Cell Biology, First Military Medical University, Guangzhou 510515, China. wangybgz@163.net
Wang
Hong
H
Guo
Hui-Yu
HY
Zhao
Yong-Zhong
YZ
Luo
Shen-Qiu
SQ
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000032:analysis; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole