title: Express detection of nonylphenol in water samples by fluorescence polarization immunoassay.
abstract: The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on: 1. omega-(4-hydroxyphenyl)nonanoic or omega-(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety.Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against omega-(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 microg mL(-1 )and assay dynamic range of 18-300 microg mL(-1).
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.
authors.forename: Julia N
authors.initials: JN
authors.lastname: Yakovleva
authors.forename: Anna Yu
authors.initials: AY
authors.lastname: Lobanova
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Shutaleva
authors.forename: Maria A
authors.initials: MA
authors.lastname: Kourkina
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Mart'ianov
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
mesh_terms: D003429:Cross Reactions; D016231:Fluorescence Polarization Immunoassay; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D010636:Phenols; D014874:Water Pollutants, Chemical; C025256:nonylphenol