Comparative analysis on the 5'-untranslated region of bovine viral diarrhea virus isolated in Korea.
Samples of 249 bovine abortuses, one intestine, and four diarrheal stools from 254 cows were collected, and bovine viral diarrhea virus (BVDV) was detected by reverse transcription-polymerase chain reaction (RT-PCR). Subsequently, virus isolation was preformed with PCR-positive samples, and then PCR product of 5(')-untranslated region (UTR) of BVDV isolate was sequenced and analyzed. Among the samples collected, 20 (from 17 bovine abortuses, one intestine, and two diarrheal stools) were positive for BVDV RT-PCR; four BVDVs (from two bovine abortuses, one intestine, and one diarrheal stool) were isolated. When the four isolates were biotyped in cell culture, one BVDV isolate from a bovine abortus was cytopathic and the others were non-cytopathic. In addition, three isolates were genotyped as BVDV-1 and one isolate from a diarrheal stool as BVDV-2. In phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea.
Park
J S
JS
Department of Veterinary Medicine Virology Lab, The Xenotransplantation Research Center, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, 151-742, Seoul, Republic of Korea.
Moon
H J
HJ
Lee
B C
BC
Hwang
W S
WS
Yoo
H S
HS
Kim
D Y
DY
Park
B K
BK
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D007723:Korea; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D020121:5' Untranslated Regions; D012367:RNA, Viral