Induction of a bystander chromosomal damage of He-ion microbeams in mammalian cells.
We report here a bystander effect in chromosomal damage using He-ion microbeam. Human-hamster hybrid cells were irradiated with a precision He-ion microbeam generated by the Columbia microbeam system. When 20% of the cells were exposed to single He ion, the incidence of cells with chromatid-type breaks detected with the PCC technique was covered wide range from 0 to 6 breaks per cell. In contrast, the distribution showed a mixed two-peak pattern, such as non-exposed and all-cell exposed patterns, under the condition of assuming no bystander effect by treating with an effective inhibitor of cell-cell communication. These findings provide clear evidence that single He-ion irradiated cells can induce bystander chromosomal alterations in neighboring cells not directly hit by He ion.
Suzuki
Masao
M
ISRL, NIRS.
Zhou
Hongning
H
Hei
Tom K
TK
Tsuruoka
Chizuru
C
Fujitaka
Kazunobu
K
D000818:Animals; D024201:Bystander Effect; D016466:CHO Cells / Q000528:radiation effects; D002467:Cell Nucleus / Q000528:radiation effects; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11 / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D020450:Heavy Ions; D006371:Helium; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D016296:Mutagenesis
D006371:Helium