title: Chromium (III) enhanced diamine silver staining of proteins and DNA in gels.
abstract: Chromium (III) enhanced the sensitivities of diamine silver staining of four proteins between 6- and 50-fold over that of the Coomassie Brilliant Blue (CBB)-chromium modified thiosulfate-silver staining method (Zhou et al. Biotechnology Letters, 2002, 24: 1561-1567). Using six dsDNA fragments, the detection limits of this new method was 10 to 30 pg per band, being 10- to 25-fold more sensitive than previous methods.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
authors.forename: Zhi-dong
authors.initials: ZD
authors.lastname: Zhou
authors.forename: Wang-yi
authors.initials: WY
authors.lastname: Liu
authors.forename: Min-qian
authors.initials: MQ
authors.lastname: Li
mesh_terms: D002857:Chromium; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D003959:Diamines; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003959:Diamines; D011506:Proteins; D012394:Rosaniline Dyes; D002857:Chromium; D012834:Silver; D004247:DNA; C004692:coomassie Brilliant Blue