title: Oxygen metabolism of the liver during an HA clamp: HV saturation and free radicals.
abstract: OBJECTIVE
  To clarify changes in the hepatic oxygen metabolism and tissue damage resulting from oxygen-derived free radical generation from polymorphonuclear cells during a hepatic arterial clamp.
  
  
  METHODS
  Subjects were 32 male Wistar rats. Hepatic tissue blood flow, and hepatic venous chemiluminescence, indicating oxygen-derived free radicals from polymorphonuclear cells, and liver lipid peroxide were measured, and hepatic and portal venous blood gas analysis were performed before and after 130 minutes of hepatic arterial clamping.
  
  
  RESULTS
  Hepatic tissue blood flow decreased by hepatic arterial clamp. The values of hepatic arterial oxygen pressure (HTBF), hepatic venous oxygen saturation (ShvO2), and O2 contents after hepatic arterial clamp were lower than those before hepatic arterial clamp (P = 0.035, 0.024, and 0.028, respectively). Hepatic venous chemiluminescence decreased and the lipid peroxide level of the liver increased by hepatic arterial clamp (P = 0.001).
  
  
  CONCLUSIONS
  ShvO2 is useful for the evaluation of hepatic oxygen metabolism and hepatic tissue blood flow during acute hepatic arterial clamp. This condition should prepare the following tissue damage due to oxygen-derived free radicals from polymorphonuclear cells.
authors.affiliation: Critical Care and Emergency Center, Yokohama City University Medical Center, 4-57 Urafune-cho, Minami-ku, Yokohama, 232-0024, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Moriwaki
authors.forename: Mitsugi
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Keiji
authors.initials: K
authors.lastname: Uchida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kosuge
authors.forename: Norihisa
authors.initials: N
authors.lastname: Karube
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toyoda
authors.forename: Toshiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Koji
authors.initials: K
authors.lastname: Kanaya
authors.forename: Goro
authors.initials: G
authors.lastname: Matsuda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamagishi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D003250:Constriction; D005609:Free Radicals / Q000097:blood; D000860:Hypoxia / Q000503:physiopathology; D007511:Ischemia / Q000503:physiopathology*; D008054:Lipid Peroxides / Q000097:blood*; D008099:Liver / Q000098:blood supply*; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000276:immunology; D018384:Oxidative Stress / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology
chemical_list: D005609:Free Radicals; D008054:Lipid Peroxides