title: General transcriptional repression by polyglutamine disease proteins is not directly linked to the presence of inclusion bodies.
abstract: By using direct immunocytochemistry of BrU incorporated to RNA in the nuclei, we evaluated the effect of mutant huntingtin and ataxin-1 on general transcription in primary cortical and cerebellar neurons. Our quantitative analyses clearly showed that these mutant polyglutamine disease proteins repress general transcription. In addition, we found that general transcription level was almost similar in inclusion body-positive and -negative neurons. The result suggests that presence of inclusion body is not essential for repressing general transcription in contrast to its reported role for suppressing specific gene transcription in the polyglutamine disease pathology.
authors.affiliation: Department of Molecular Therapeutics, Tokyo Metropolitan Institute for Neuroscience, 2-6, Musashi-dai, Fuchu, Tokyo 183-8526, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Hoshino
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tagawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Okuda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okazawa
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000067696:Ataxin-1; D000067528:Ataxins; D001973:Bromodeoxyuridine / Q000378:metabolism; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D020271:Heredodegenerative Disorders, Nervous System / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000071058:Huntingtin Protein; D002479:Inclusion Bodies / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C000593749:ATXN1 protein, human; D000067696:Ataxin-1; D000067528:Ataxins; C000593751:Atxn1 protein, rat; D002087:Butyrates; C086055:HTT protein, human; C499524:Htt protein, rat; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010455:Peptides; D012097:Repressor Proteins; C097188:polyglutamine; D001973:Bromodeoxyuridine