Bioconversion of compactin into pravastatin by Streptomyces sp.
Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l(-1) from 750 mg compactin l(-1) in 24 h. By intermittent feeding of compactin into the culture medium, both the compactin concentration and its conversion increased to 2000 mg l(-1) and 1000 mg pravastatin l(-1), respectively, with the conversion rate of 10 mg l(-1) h(-1). Continuous feeding of compactin increased production of pravastatin to 15 mg l(-1) h(-1).
Park
Joo-Woong
JW
Biotechnology Laboratory, Youngjin Pharmaceutical Co. Ltd., Pyungtaek-Si 451-860, Korea.
Lee
Joo-Kyung
JK
Kwon
Tae-Jong
TJ
Yi
Dong-Hee
DH
Kim
Young-Jun
YJ
Moon
Seong-Hoon
SH
Suh
Hyun-Hyo
HH
Kang
Sang-Mo
SM
Park
Yong-Il
YI
D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D017035:Pravastatin / Q000096:biosynthesis*; D013302:Streptomyces / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
C012258:mevastatin; D003577:Cytochrome P-450 Enzyme System; D008148:Lovastatin; D017035:Pravastatin