Acquisition of host plasmin activity by the Swine pathogen Streptococcus suis serotype 2.
In this study, the plasminogen-binding activity of Streptococcus suis serotype 2 was investigated. Bound human plasminogen was activated by purified streptokinase, urokinase, or Streptococcus dysgalactiae subsp. equisimilis culture supernatant. Both human and porcine plasminogen were bound by S. suis. Binding was inhibited by epsilon-aminocaproic acid, and the plasminogen receptor was heat and sodium dodecyl sulfate resistant. One of the receptors was identified as glyceraldehyde-3-phosphate dehydrogenase. S. suis-associated plasmin activity was capable of activating free plasminogen, which in turn could contribute to degradation of fibronectin. This is the first report on the plasminogen-binding activity of S. suis. Further studies may reveal a contribution of this activity to the virulence of S. suis.
Jobin
Marie-Claude
MC
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada.
Brassard
Julie
J
Quessy
Sylvain
S
Gottschalk
Marcelo
M
Grenier
Daniel
D
D000818:Animals; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005341:Fibrinolysin / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010958:Plasminogen / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000254:growth & development / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D003470:Culture Media; D010958:Plasminogen; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010960:Plasminogen Activators; D005341:Fibrinolysin