Development of a microtiter plate ELISA and a dipstick ELISA for the determination of the organophosphorus insecticide fenthion.
In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA showed an IC(50) value of 1.2 microg/L with a detection limit of 0.1 microg/L. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides. The use of the dipstick format using Immunodyne as a support membrane allowed the quick visual detection of fenthion in concentrations >10 microg/L. The IC(50) value of the dipstick format using reflectance detection was 15 microg/L with a detection limit of 0.5 microg/L. The recoveries of fenthion from spiked vegetable samples using the two formats without any prior enrichment or cleanup steps were 87-116%.
Cho
Young Ae
YA
Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea.
Kim
Yoo Jung
YJ
Hammock
Bruce D
BD
Lee
Yong Tae
YT
Lee
Hye-Sung
HS
D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005284:Fenthion / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D011786:Quality Control; D011934:Reagent Strips
D007306:Insecticides; D011934:Reagent Strips; D005284:Fenthion