title: Relationships in subtribe Diocleinae (Leguminosae; Papilionoideae) inferred from internal transcribed spacer sequences from nuclear ribosomal DNA.
abstract: The complete sequences of nuclear ribosomal DNA (nrDNA) internal transcribed spacer regions (ITS/5.8S) were determined for species belonging to six genera from the subtribe Diocleinae as well as for the anomalous genera Calopogonium and Pachyrhizus. Phylogenetic trees constructed by distance matrix, maximum parsimony and maximum likelihood methods showed that Calopogonium and Pachyrhizus were outside the clade Diocleinae (Canavalia, Camptosema, Cratylia, Dioclea, Cymbosema, and Galactia). This finding supports previous morphological, phytochemical, and molecular evidence that Calopogonium and Pachyrhizus do not belong to the subtribe Diocleinae. Within the true Diocleinae clade, the clustering of genera and species were congruent with morphology-based classifications, suggesting that ITS/5.8S sequences can provide enough informative sites to allow resolution below the genus level. This is the first evidence of the phylogeny of subtribe Diocleinae based on nuclear DNA sequences.
authors.affiliation: Laboratório de Citogenética e Genética Molecular, Departamento de Biologia, Bloco 906, Centro de Ciências, Universidade Federal do Ceará (UFC), Av. Humberto Monte, s/n. 60.451-970, Fortaleza-Ceará, Brazil.
authors.forename: Eduardo S
authors.initials: ES
authors.lastname: Varela
authors.forename: João P M S
authors.initials: JP
authors.lastname: Lima
authors.forename: Alexsandro S
authors.initials: AS
authors.lastname: Galdino
authors.forename: Luciano da S
authors.initials: Lda S
authors.lastname: Pinto
authors.forename: Walderly M
authors.initials: WM
authors.lastname: Bezerra
authors.forename: Edson P
authors.initials: EP
authors.lastname: Nunes
authors.forename: Maria A O
authors.initials: MA
authors.lastname: Alves
authors.forename: Thalles B
authors.initials: TB
authors.lastname: Grangeiro
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D016384:Consensus Sequence; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D007887:Fabaceae / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal