title: Development of a sheathless interface between reversed-phase capillary HPLC and ICPMS via a microflow total consumption nebulizer for selenopeptide mapping.
abstract: A sheathless interface based on a total consumption micronebulizer operating at flow rates in the range 0.5-7.5 microL min(-1) was developed between capillary HPLC and ICPMS. It allowed the efficient nebulization and transport into the plasma of mobile phases containing up to 100% organic solvent without either cooling the spray chamber or oxygen addition. The coupled system was applied to selenopeptide mapping in a protein fraction isolated from a selenized yeast extract. The detection limits were 150 (80Se) and 200 fg (82Se) for a quadrupole instrument with and without a collision cell, respectively, which is a factor 100-150 less than that reported elsewhere for HPLC-ICPMS. The minimal peak broadening ( approximately 5 s at the half-height) allowed baseline resolution of a mixture containing more than 30 selenopeptides, many of which could not be separated using the conventional HPLC-ICPMS coupling.
authors.affiliation: Group of Bio-Inorganic Analytical Chemistry, CNRS UMR 5034, Hélioparc, 2, Av. Pr. Angot, F-64053 Pau, France.
authors.forename: Dirk
authors.initials: D
authors.lastname: Schaumlöffel
authors.forename: Jorge
authors.initials: J
authors.lastname: Ruiz Encinar
authors.forename: Ryszard
authors.initials: R
authors.lastname: Łobiński
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008832:Microchemistry / Q000379:methods; D009330:Nebulizers and Vaporizers; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D012643:Selenium / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D010455:Peptides; D012643:Selenium