title: Control of mouse hils1 gene expression during spermatogenesis: identification of regulatory element by transgenic mouse.
abstract: Histone H1-like protein in spermatids 1 (Hils1) is a testis- specific histone H1-like protein exclusively expressed in haploid spermatids and should be involved in chromatin remodeling during mouse spermatogenesis. Spatial and temporal regulation of the hils1 gene expression would be critical for the formation of functional sperm, controlled at both transcriptional and translational levels. Previously, we reported that transcripts of the hils1 gene are exclusively expressed in mouse testis from 23 days of age whereas the Hils1 protein is not detected until 28 days of age, suggesting that hils1 is a member of a class of translationally regulated genes. By analyzing transgenic mice, we could demonstrate that 318-base pair (bp) 5'-proximal region corresponding to the first 70-bp proximal TATA-less promoter, and 248 bp of 5'-untranslated region is sufficient to confer testis- and spermatid-specific transcription as well as posttranscriptional control of the mouse hils1 gene in vivo.
authors.affiliation: Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Iguchi
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yamada
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nishimura
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Nishimune
mesh_terms: D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D005453:Fluorescence; D005786:Gene Expression Regulation; D016678:Genome; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D007202:Indicators and Reagents / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; C494057:Hils1 protein, mouse; D007202:Indicators and Reagents; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins