title: In vitro protein refolding by chromatographic procedures.
abstract: In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Su
authors.forename: Jan-Christer
authors.initials: JC
authors.lastname: Janson
mesh_terms: D018833:Chaperonins / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D017510:Protein Folding; D020673:Protein Renaturation; D014508:Urea / Q000737:chemistry
chemical_list: D008081:Liposomes; D011092:Polyethylene Glycols; D014508:Urea; D018833:Chaperonins