[Polymorphism of the STR system LPL in the Silesian population].
This paper shows the results of locus LPL in the population of Upper Silesia. The examinations were performed in a group of 147 unrelated adults. The following gene frequencies were observed: LPL*9 = 0.0578; LPL* 10 = 0.4048; LPL* 11 = 0.2517, LPL* 12 = 0.2449 i LPL* 13 = 0.0408. DNA was isolated using Blood DNA Prep Plus of A&A Biotechnology. Amplification was carried out in a Perkin Elmer GeneAmp PCR System 2400 thermal cycler, using the GenePrint STR System LPL (8p22) of Promega Corporation, Madison, WI, USA. PCR products were electrophoretically separated by high-resolution polyacrylamide gel--GDG of Perkin Elmer. Gels were stained by the silver method. Test chi 2, exact and Carmody's were used for statistical estimation. The identification values of the system are: PD, Ht, PM, MEC, PIC, MEP. The analysis of studies has shown that the examined population is in the genetic equilibrium conformable to the Hardy-Weinberg's principle. The estimated gene frequencies are similar to those observed in two Polish populations.
Droździok
Kornelia
K
Katedry Medycyny Sadowej Slaskiej AM w Katowicach.
Kabiesz
Jadwiga
J
D000328:Adult; D000483:Alleles; D001801:Blood Stains; D005787:Gene Frequency / Q000235:genetics*; D005799:Genes, Dominant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics*; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012016:Reference Values; D020080:Tandem Repeat Sequences / Q000235:genetics*; D044465:White People / Q000235:genetics
D005819:Genetic Markers; D008071:Lipoprotein Lipase