title: Evolutionarily conserved expression pattern and trans-regulating activity of Xenopus p51/p63.
abstract: p51/p63, a member of the p53 gene family, is structurally conserved among a wide range of organisms, although the transactivator (TA) and N-terminally truncated (deltaN) isotype producing property seems to vary. Since p51/p63 is thought to play important roles in skin, limb, and craniofacial development in mammals, we examined Xenopus laevis larval and adult tissues for expression of p51/p63. Temporal analyses indicated enhanced transcription of the deltaN form of p51/p63 in premetamorphosis phase (at stage 44-48). p51/p63-positive cells in the inner layer of larval skin expanded to the suprabasal layers during the stratification. The epithelium of limb buds and the maxillofacial ectodermal tissues in tadpoles had a high level expression of p51/p63. The cloned deltaN-A/gamma type Xenopus p51/p63 exhibited a dominant-negative activity against the human TA-A/gamma isotype in a reporter assay. These results suggest that tissue-specific p51/p63-inducing mechanism and isotype-specific transcriptional regulator activities of p51/p63 are conserved between mammals and frogs.
authors.affiliation: Molecular Pathophysiology, Department of Moleculo-genetic Sciences, Division of Biomedical Laboratory Sciences, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Tomimori
authors.forename: Iyoko
authors.initials: I
authors.lastname: Katoh
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Kurata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okuyama
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kamiyama
authors.forename: Yoji
authors.initials: Y
authors.lastname: Ikawa
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D007814:Larva / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014018:Tissue Distribution; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D014157:Transcription Factors; D015533:Transcriptional Activation; D014162:Transfection; D025521:Tumor Suppressor Proteins; D014981:Xenopus / Q000254:growth & development / Q000378:metabolism*; D029867:Xenopus Proteins
chemical_list: D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C488640:TP63 protein, Xenopus; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D029867:Xenopus Proteins