title: Plasma circulating fibrinogen stability and moderate beer consumption.
abstract: MODERATE BEER CONSUMPTION (MBC) IS CARDIOPROTECTIVE: it positively influences plasma lipid levels and plasma antioxidant activity in beer-consuming individuals. The connection between MBC and blood coagulation is not clearly defined. Forty-two volunteers were equally divided into experimental (EG) and control (CG) groups following coronary bypass surgery. For 30 consecutive days, only patients of the EG consumed 330 mL of beer per day (about 20 g of alcohol). A comprehensive clinical investigation of 42 patients was done. Blood samples were collected before and after the investigation for a wide range of laboratory tests. The plasma fibrinogen was denatured with 8 M urea and intrinsic fluorescence (IF), hydrophobicity and differential scanning calorimetry (DSC) were used to reveal possible qualitative changes. After 30 days of moderate beer consumption, positive changes in the plasma lipid levels, plasma anticoagulant and plasma antioxidant activities were registered in patients of the EG group. In 17 out of 21 patients of the same group, differences in plasma circulating fibrinogen's (PCF), secondary and tertiary structures were found. The stability of fibrinogen, expressed in thermodynamic parameters, has shown that the loosening of the structure takes place under ethanol and urea denaturation. Also fluorescence stability of PCF was decreased. No changes in the lipid levels, anticoagulant and antioxidant activity or changes in PCF were detected in patients of CG. In conclusion, for the first time after a short term of moderate beer consumption some qualitative changes in the plasma circulating fibrinogen were detected: differences in the emission peak response, fluorescence intensity and all thermodynamic data. Together, with the decrease in the PCF concentration it may lead to an elevation of the blood anticoagulant activity.
authors.affiliation: Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University - Hadassah Medical School, P.O.B. 12065, Jerusalem 91120, Israel. gorin@cc.huji.ac.il
authors.forename: Shela
authors.initials: S
authors.lastname: Gorinstein
authors.forename: Abraham
authors.initials: A
authors.lastname: Caspi
authors.forename: Marina
authors.initials: M
authors.lastname: Zemser
authors.forename: Imanuel
authors.initials: I
authors.lastname: Libman
authors.forename: Ivan
authors.initials: I
authors.lastname: Goshev
authors.forename: Simon
authors.initials: S
authors.lastname: Trakhtenberg
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis; D001515:Beer / Q000009:adverse effects; D002152:Calorimetry, Differential Scanning; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000601:surgery; D004355:Drug Stability; D005340:Fibrinogen / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D000975:Antioxidants; D008055:Lipids; D005340:Fibrinogen