title: [Clinical and experimental study of 38 cases with trisomy 8].
abstract: OBJECTIVE
  To study the role of trisomy 8 in pathogenesis and progression of hematologic disease with trisomy 8.
  
  
  METHODS
  The clinical data on 38 cases with trisomy 8 were investigated retrospectively. Fluorescence in situ hybridization (FISH) using Spectrum Orange labeled chromosome 8 centromere specific probe was carried out to detect trisomy 8 in 10 cases.
  
  
  RESULTS
  Thirty-two of 38(84.2%) cases with trisomy 8, and fourteen of 17(82.4%) cases with trisomy 8 as the sole chromosome aberration were myeloid disorders such as myelodysplastic syndrome (MDS), acute myelocytic leukemia (AML), chronic myelocytic leukemia (CML). The incidence of trisomy 8 was higher in myeloid disease than in lymphocytic disease (5% vs 1.3%); the incidence of trisomy 8 was higher in acute monocytic leukemia than in other AML (6.1% vs 2.4%), and the incidence of trisomy 8 in chronic myelomonocytic leukemia( CMML) was higher than that in other myelodysplastic syndrome (MDS) (25% vs 13.2%); 17 cases had trisomy 8 as the sole chromosome aberration, 21 cases had other additional chromosome aberrations. The chromosome aberration was confirmed by FISH in 10 cases with trisomy 8 as the sole chromosome aberration. Eleven cases were treated with chemotherapy, among them only 10 cases data were available. Seven cases acquired complete remission but 3 of them were M3, the other 3 cases had no response after two courses of chemotherapy.
  
  
  CONCLUSIONS
  Trisomy 8 may play an important role in the pathogenesis and progression of the hematological disease, especially myeloid disease. Trisomy 8 might be related with differentiation abnormality of monocyte.
authors.affiliation: The First Affiliated Hospital, Institute of Hematology, Zhejiang Universitry, Hangzhou, Zhejiang, 310003 PR China. xuweilai2001@sohu.com
authors.forename: Wei-lai
authors.initials: WL
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Zhi-mei
authors.initials: ZM
authors.lastname: Chen
authors.forename: Ji-yu
authors.initials: JY
authors.lastname: Lou
authors.forename: Yun-biao
authors.initials: YB
authors.lastname: Yu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D014314:Trisomy