Isolation and characterization of a Pti1 homologue from soybean.
A full-length gene GmPti1 was identified from soybean in an EST sequencing project by its homology to tomato Pti1. It encoded a protein of 366 amino acids. RT-PCR analysis showed that the GmPti1 expression was induced by salicylic acid and wounding. The deduced amino acid sequence had a Ser/Thr/Tyr kinase domain. GmPti1 protein was expressed in E. coli as an MBP fusion, purified by amylose resin and examined for its autophosphorylation ability. The phosphorylation assay in vitro showed that GmPti1 had kinase activity in the presence of Mn2+. These results demonstrated that GmPti1 represented a new Pti1-like gene, unlike the two published genes sPti1a and sPti1b, which encoding proteins had no autophosphorylation ability.
Tian
Ai-Guo
AG
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Luo
Guang-Zuo
GZ
Wang
Yong-Jun
YJ
Zhang
Jin-Song
JS
Gai
Jun-Yi
JY
Chen
Shou-Yi
SY
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D010940:Plant Proteins; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics*
D017931:DNA Primers; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases