Ecabet sodium attenuates reactive oxygen species produced by neutrophils after priming with bacterial lipopolysaccharides.
The pathogenic roles of reactive oxygen species (ROS) have been implicated in ulcerative colitis (UC). The aim of this study was to examine the effects of ecabet sodium on ROS produced by human neutrophils, particularly after being primed by bacterial lipopolysaccharides (LPS). Neutrophils were isolated from six healthy volunteers. Each well of a 96-well microplate received neutrophil suspension (1.0 x 10(5) cells) and the plates were incubated at 37 degrees C for 30 min with or without E. coli LPS (f.c. 0.001 ng/ micro L). Ecabet sodium (f.c. 0-5.0 mg/mL) was added before starting or after finishing the incubation. Neutrophils were stimulated by opsonized zymosan (OZ; 1.0 mg/mL) or calcium ionophore (A21837; 0.3 micro mol/L) and luminol-dependent chemiluminescence response was measured using a Lumi Box H-1000. Ecabet sodium attenuated ROS production at a concentration of 5.0 mg/mL (p < 0.05) in LPS-primed neutrophils. However, attenuating effects were not significantly different when ecabet sodium was added before or after the incubation with E. coli LPS. Ecabet sodium may have some attenuating effects on ROS produced by human neutrophils even after neutrophils are primed by bacterial LPS. These results may explain, in part, the therapeutic effects of ecabet sodium for UC.
Munakata
Wataru
W
Department of Hygiene, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Liu
Qiang
Q
Shimoyama
Tadashi
T
Sawaya
Manabu
M
Umeda
Takashi
T
Sugawara
Kazuo
K
D045784:Abietanes / Q000494:pharmacology*; D004926:Escherichia coli / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*
D045784:Abietanes; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; C061681:ecabet; D008165:Luminol