Noradrenaline and its end metabolite 3-methoxy-4-hydroxyphenylglycol inhibit lymphocyte chemotaxis: role of alpha- and beta-adrenoreceptors.
The capacity of noradrenaline (NA) and its end metabolite 3-methoxy-4-hydroxyphenylglycol (MHPG) to modulate the chemotaxis of lymphocytes from a primary immunocompetent organ (thymus) and a secondary one (spleen) was investigated over a range of concentrations from 10(-12) M to 10(-5) M. Lymphocyte chemotaxis was evaluated in a Boyden chamber. The results indicated that 10(-5) M of NA inhibits the chemotaxis of lymphocytes from both the immunocompetent organs studied, and that this effect is blocked by either propranolol (10(-6) M) or phentolamine (10(-5) M). Similarly, 10(-5) M of MHPG induced a decrease in the chemotaxis capacity of the lymphocytes. In conclusion, high physiological concentrations of NA and its end metabolite modulate the mobility of lymphocytes, and the participation of both alpha and beta adrenoreceptors is necessary, showing a new aspect of neuroimmune interactions.
García
Juan José
JJ
Department of Physiology, University of Extremadura, Badajoz, Spain.
del Carmen Sáez
María
M
De la Fuente
Mónica
M
Ortega
Eduardo
E
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002633:Chemotaxis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008734:Methoxyhydroxyphenylglycol / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009638:Norepinephrine / Q000494:pharmacology*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D000316:Adrenergic alpha-Agonists; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D008734:Methoxyhydroxyphenylglycol; D011433:Propranolol; D009638:Norepinephrine; D010646:Phentolamine