[Analysis of scratching behavior in a picryl chloride induced atopic dermatitis model in mice].
To elucidate the actual state of scratching behavior of NC mice noted when PiCl-induced dermatitis occurs, the circadian rhythm in scratching behavior of this mouse model was examined, and the time when scratching behavior, which is useful to evaluate the severity of itch, occurs was assessed. A steroid drug (Prednisolone ointment), which has been confirmed to inhibit dermatitis from worsening, was used to examine whether or not, or how it inhibits scratching behavior in this mouse model. It became clear that scratching behavior increased during a period from the evening to the night in the animals which had not been sensitized (normal animals); compared with the day time, scratching behavior occurred more often in the nighttime. It also became clear that scratching behavior increased in the animals with PiCl-induced dermatitis increase in the frequency of induction of dermatitis, and Prednisolone ointment significantly inhibited scratching behavior in the animals in which dermatitis had been induced with PiCl six times. From these results, it can be said that scratching behavior increases in PiCl-induced mouse atopic dermatitis models correlatively with the increase in the frequency of induction of dermatitis, and steroid drugs decrease the frequency of the scratching behavior. In conclusion, it is strongly suggested that this mouse model is useful for development of therapeutic methods and novel medicinal drugs for atopic dermatitis.
Hirasawa
Yasushi
Y
Nihon Bioresearch Inc. y-hirasawa@nbr.co.jp
Ori
Kazutomo
K
Ohtsu
Shoko
S
Matsui
Yukari
Y
Shimizu
Masayoshi
M
Kyuki
Kohei
K
Higo
Shoichi
S
D000818:Animals; D001522:Behavior, Animal; D003876:Dermatitis, Atopic / Q000503:physiopathology*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010853:Picryl Chloride; D011537:Pruritus / Q000503:physiopathology*
D010853:Picryl Chloride