A diabetes-related epitope of GAD65: a major diabetes-related conformational epitope on GAD65.
The 65-kDa isoform of glutamic acid decarboxylase (GAD65) is a major autoantigen in type 1 diabetes, and most patients have serum antibodies reactive with conformational epitopes on the GAD65 molecule. The aims of this study were to prepare mutants of GAD65 to further localize the type 1 diabetes epitope in the region of the PEVKEK loop of GAD65 and to identify the particular amino acids within the epitope that are recognized by autoimmune diabetes sera.
Myers
Mark A
MA
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Fenalti
Gustavo
G
Gray
Robyn
R
Scealy
Marita
M
Tong
Jonathan C
JC
El-Kabbani
Ossama
O
Rowley
Merrill J
MJ
D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006801:Humans; D007527:Isoenzymes / Q000276:immunology*; D011487:Protein Conformation
D001323:Autoantibodies; D000939:Epitopes; D007527:Isoenzymes; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2