Tissue immunoassay for 19F-tagged 5-hydroxytryptophan.
A new tool for magnetic resonance, L-6-heptafluorobutyryl-5-hydroxytryptophan, was synthesized and investigated using an antibody to perfluoroalkyl moieties developed previously. To be useful as an imaging agent, the compound must cross the blood brain barrier and then be concentrated in vesicles in serotonergic neurons in order to accumulate in sufficient quantity for in vivo detection to be possible. The novel imaging compound was administered in ova to domestic chicks (Gallus domestics) to investigate the bioavailability and uptake dynamics of the compound in this model organism. Typical immunoassay methods were ineffective, so a new technique was developed which binds amines and amino acids to the walls of acid-functionalized cuvettes. The first study established the presence of higher quantities of the tags in neural and liver tissue than in heart tissue. A second study investigated regional differences, with the midbrain containing more tagged compounds than the frontal lobe sample, and the frontal lobe sample containing more than the occipital or cerebellum samples. These studies demonstrate that the compound follows the pathway of endogenous serotonin. A third study investigated uptake dynamics of the novel compound. Maximum concentration of the tagged molecule in the brain was achieved three days after injecting Incubation Day 14 eggs, suggesting that it bioaccumulates in vivo. This new immunoassay technique used to detect the novel compound in tissue samples demonstrated good repeatability.
Dingman
Sherry
S
Marist College, Poughkeepsie, New York 12601, USA. Sherry.Dingman@marist.edu
Hurlburt
Lara
L
Thomas
Rhys
R
Guo
Congyuan
C
D006916:5-Hydroxytryptophan / Q000031:analogs & derivatives / Q000032:analysis / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D000818:Animals; D001812:Blood-Brain Barrier / Q000196:embryology / Q000378:metabolism; D001923:Brain Chemistry; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism; D002642:Chick Embryo / Q000378:metabolism*; D005461:Fluorine; D005466:Fluorocarbons / Q000032:analysis / Q000138:chemical synthesis / Q000493:pharmacokinetics; D005625:Frontal Lobe / Q000196:embryology / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D007554:Isotopes; D008099:Liver / Q000196:embryology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008636:Mesencephalon / Q000196:embryology / Q000378:metabolism; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
C488379:6-heptafluorobutyryl-5-hydroxytryptophan; D005466:Fluorocarbons; D007554:Isotopes; D005461:Fluorine; D012701:Serotonin; D006916:5-Hydroxytryptophan