Biophysical properties of Kv3.1 channels in SH-SY5Y human neuroblastoma cells.
Biophysical properties of delayed rectifier K channels in the human neuroblastoma SH-SY5Y were established using patch clamp recordings. The whole cell K+ conductance activated at membrane potentials positive to -20 mV. The midpoint of current activation was 9.6 +/- 5.1 mV, the equivalent charge was 3.7 +/-.6. Whole-cell currents inactivated slightly with time constants of 700 ms and 5 s. The K+ currents were sensitive to micromolar concentrations of TEA and 4-aminopyridine. RT-PCR experiments amplified a cDNA fragment specific for human Kv3.1 channels. Activation gating parameters in outside-out patches were shifted by approximately 14 mV in the hyperpolarizing direction.
Friederich
P
P
Universitätsklinik für Anästhesiologie, University of Hamburg, Martinistrasse 52, 20251 Hamburg, FRG. patrick.friederich@zmnh.uni-hanburg.de
Dilger
J P
JP
Isbrandt
D
D
Sauter
K
K
Pongs
O
O
Urban
B W
BW
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002460:Cell Line; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D016678:Genome; D006801:Humans; D007700:Kinetics; D009479:Neuropeptides / Q000737:chemistry / Q000502:physiology*; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D011188:Potassium / Q000737:chemistry; D015221:Potassium Channels / Q000737:chemistry / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051670:Shaw Potassium Channels; D013997:Time Factors
D018076:DNA, Complementary; D009479:Neuropeptides; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051670:Shaw Potassium Channels; D015761:4-Aminopyridine; D011188:Potassium