title: Defective RNA-mediated c-myc gene silencing pathway in Burkitt's lymphoma.
abstract: Keeping in view the fact that molecular basis of Burkitt's lymphoma (BL) is poorly understood, we attempted to explore the small interfering RNA (siRNA) mediated c-myc gene regulation using BL-derived EB-3 cell line as archetype cellular model. Such a study revealed that EB-3 cells possess 4-fold higher expression of Dicer gene coupled with 2-fold higher activity of RNA polymerase III than that observed in normal human lymphocytes. siRNAs derived from EB-3 cells had the inherent capacity to suppress c-myc gene expression in normal cells but not in native cells. Based on these findings we have proposed a novel RNA-mediated c-myc gene regulation pathway that may be responsible for BL.
authors.affiliation: Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160 012, India. dkaul_24@hotmail.com
authors.forename: Deepak
authors.initials: D
authors.lastname: Kaul
authors.forename: Kavleen
authors.initials: K
authors.lastname: Sikand
mesh_terms: D002051:Burkitt Lymphoma / Q000235:genetics*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D020868:Gene Silencing; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008954:Models, Biological; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012320:RNA Polymerase III / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D012313:RNA; D012320:RNA Polymerase III