title: [Study on DNA methylation status of WT1 gene promoter in leukemia cell].
abstract: OBJECTIVE
  To analyse the WT1 expression and its DNA methylation status of its promoter domain.
  
  
  METHODS
  The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
  
  
  RESULTS
  WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.
  
  
  CONCLUSIONS
  DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.
authors.affiliation: Department of Hematology, PLA General Hospital, Beijing 100853, China.
authors.forename: Quan-shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Han
authors.forename: Chun-ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Fang-ding
authors.initials: FD
authors.lastname: Lou
mesh_terms: D045744:Cell Line, Tumor; D019175:DNA Methylation; D016162:Genes, Wilms Tumor; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic