Age-dependent decline of in vitro migration (basal and stimulated by IGF-1 or insulin) of human vascular smooth muscle cells.
Since biological aging causes a decrease in functions such as cell proliferation, we have studied the possible effect of age on the migration capacity of human vascular smooth muscle cells (SMCs). To this aim, the migration activity of cultured SMCs from arteries of male human donors ranging in age from 43-77 years was determined in a Boyden chamber, under basal conditions and after insulin-like growth factor-1 (IGF-1) or insulin stimulation. Migration activity decreased with donor age (r2 = 87%, 85%, and 78%, respectively). IGF-1 and insulin significantly reduced the age-dependent relationship observed in basal conditions, so that, comparing young with old, both IGF-1 and insulin stimulated SMC migration similarly, although the effect of age remained in absolute terms. In this article, we conclude that the age-dependent decline of migration activity--similar to what has already been shown for SMC proliferation--may be part of the biological ageing phenotype, which is not overcome by hormone stimulation.
Ruiz-Torres
Antonio
A
University Research Institute of Ageing, Hospital de la Princesa, Madrid, Spain. aruto@arrakis.es
Lozano
Rosario
R
Melón
José
J
Carraro
Raffaele
R
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012307:Risk Factors; D012680:Sensitivity and Specificity
D007328:Insulin; D007334:Insulin-Like Growth Factor I