[Evaluation criterion for nuclear chromatin state of human spermatozoa using flow cytometry--a fertility test].
On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.
Semenova
E V
EV
St. Petersburg Nuclear Physics Institute RAS, St. Petersburg.
Drobchenko
E A
EA
Filatov
M V
MV
D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D015203:Reproducibility of Results; D013094:Spermatozoa / Q000166:cytology*
D002843:Chromatin