title: Studies of the Escherichia coli Rsd-sigma70 complex.
abstract: Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. westbll@rockefeller.edu
authors.forename: Lars F
authors.initials: LF
authors.lastname: Westblade
authors.forename: Leopold L
authors.initials: LL
authors.lastname: Ilag
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Powell
authors.forename: Annie
authors.initials: A
authors.lastname: Kolb
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.forename: Stephen J W
authors.initials: SJ
authors.lastname: Busby
mesh_terms: D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism; D012808:Sigma Factor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C112239:Rsd protein, E coli; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases