Susceptibility of non-linear systems as an approach to metabolic responses.
BACKGROUND
Recent developments of several analytical techniques and fast sampling procedures are making it possible to study non-linear dynamics on an automated basis. Approaches that suggest experimental setups and fully exploit the data are needed. To prevent unreliable fits of parameters to experimental data, model-independent methods would be advantageous. One such method consists in representing the response of a system by means of a transformation or functional, of an excitation, i.e. a flux of a metabolite.


RESULTS
A functional of unknown form can be expanded in series if its functional derivatives are known. An algorithm for calculating such generalized derivatives from impulse-perturbation experiments was developed. The only assumption was that the implicit kinetics is time-independent, i.e. the system is time-invariant. The method is illustrated on a part of fructose catabolism. A reaction network that involves 14 metabolites and 11 enzymes and includes several branches and feedback loops is considered. It is shown that the method provides good approximations to the responses of this complex system.


BACKGROUND
FUNDER is available from http://bbm1.ucm.es/torralba/funder/down/


BACKGROUND
http://bbm1.ucm.es/torralba/funder/
Torralba
Antonio S
AS
Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, 28040, Madrid, Spain. torralba@bbm1.ucm.es
D000465:Algorithms; D002384:Catalysis; D005246:Feedback / Q000502:physiology*; D005632:Fructose / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology; D008660:Metabolism / Q000502:physiology*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000502:physiology; D017711:Nonlinear Dynamics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000502:physiology; D013269:Stochastic Processes
D009097:Multienzyme Complexes; D005632:Fructose