Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors.
Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g(-1) astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l(-1), astragaloside IV 0.9 mg g(-1)). Polysaccharide yields were similar amongst the different bioreactors (range 25-32 mg g(-1)). The active constituent content of the cells approached that of plant extracts, indicating that large scale hairy root cultures of A. membranaceus has the potential to provide an alternative to plant crops without compromising yield or pharmacological potential.
Du
M
M
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Box 67, No. 530 Lingling Road, Shanghai 200032, PR China. wxj_dm@hotmail.com
Wu
X J
XJ
Ding
J
J
Hu
Z B
ZB
White
K N
KN
Branford-White
C J
CJ
D027885:Astragalus propinquus / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D010865:Pilot Projects; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D012503:Saponins / Q000096:biosynthesis*; D014315:Triterpenes
D011134:Polysaccharides; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A