title: [Manifestation of glutathione S-transferase GSTM1 and GSTT1 in female patients with bleomycin-positive chromosome instability].
abstract: OBJECTIVE
  The purpose of this research is to assess the incidence of gene polymorphisms coding the GSTM1 and GSTT1 enzymes in a population of female patients with chromosome instability.
  
  
  METHODS
  The PCR method was used to determine the genotype for GSTM1 and GSTT1. The breaks per cell and the percentage of damaged cells were calculated. The separation point used to diagnose chromosome instability in tested females was assumed to be 2.5.
  
  
  RESULTS
  In a tested population of 85 females with chromosome instability, the deletion of both the alleles of the GSTT1 gene was observed in 22 females (25% of the group) and deletion of both the alleles of the GSTM1 gene was observed in 42 females (53% of the group). In addition, the incidence of individual genes was calculated for the tested population. The X2 test showed that the differences between the observed and expected values of the tested genes were statistically immaterial, i.e. the likelihood of randomness for these differences exceeded 99%.
  
  
  CONCLUSIONS
  No relationship between the manifestation of genotypes for the GSTM1 and GSTT1 glutathione S-transferases and an increased chromosome instability confirmed with the bleomycin test was proven for a population of females with a neoplastic risk.
authors.affiliation: Katedry i Kliniki Perinatologii i Ginekologii SlAM w Katowicach.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Waksmański
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kamiński
mesh_terms: D000328:Adult; D001761:Bleomycin; D043171:Chromosomal Instability / Q000235:genetics; D025063:Chromosome Disorders / Q000201:enzymology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D001761:Bleomycin; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase