In vitro protein refolding by chromatographic procedures.
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
Li
Ming
M
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China.
Su
Zhi-Guo
ZG
Janson
Jan-Christer
JC
D018833:Chaperonins / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D017510:Protein Folding; D020673:Protein Renaturation; D014508:Urea / Q000737:chemistry
D008081:Liposomes; D011092:Polyethylene Glycols; D014508:Urea; D018833:Chaperonins