title: Immunohistochemical study of chronological and photo-induced aging skins using the antibody raised against D-aspartyl residue-containing peptide.
abstract: BACKGROUND
  Biologically uncommon D-aspartyl residues have been reported in the elderly tissues such as tooth, eye lens, aorta, and brain. We have previously prepared the antibody against D-aspartyl residue-containing peptide and found that it reacted with elastotic material of actinic elastosis.
  
  
  METHODS
  Immunoreactivity of the normal skins obtained from sun-exposed and sun-protected skins of varied ages with this antibody was studied.
  
  
  RESULTS
  In the sun-exposed skins, the antibody showed negative reaction with the skin specimens of young donors, whereas it reacted with elastotic materials of actinic elastosis of the elderly. In the sun-protected skins, the antibody recognized elastic fiber-like structures and inner layer of vessels found from the mid to lower dermis of old donors but showed no positive reaction to skin specimens of young donors.
  
  
  CONCLUSIONS
  The results suggest that the antibody is a potent marker for chronological and ultraviolet (UV)-induced skin aging. Unusual eosinophilic bodies seen in the superficial dermis in the sun-exposed area of the elderly skins were also immunoreactive with the antibody, suggesting that the eosinophilic bodies resulted from UV-induced skin damage.
authors.affiliation: Department of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Miura
authors.forename: Norihiro
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Takehiko
authors.initials: T
authors.lastname: Komatsu
authors.forename: Shingo
authors.initials: S
authors.lastname: Tajima
authors.forename: Akira
authors.initials: A
authors.lastname: Kawada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Noriko
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000528:radiation effects*; D015415:Biomarkers / Q000032:analysis; D002648:Child; D026603:D-Aspartic Acid / Q000276:immunology / Q000378:metabolism*; D005148:Facial Dermatoses / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D026603:D-Aspartic Acid