Identification of in vivo pellicle constituents by analysis of serum immune responses.
Human acquired enamel pellicle is composed of molecules that selectively adsorb from saliva onto tooth surfaces and provides a protective interface between the tooth enamel and the oral environment. To identify the micro-amounts of components present in pellicle, we immunized mice with in vivo-formed human acquired enamel pellicle and analyzed the serum immune responses. Selective reactivities of the serum (OD > 1.0 above background) against albumin, amylase, carbonic anhydrase II, sIgA, IgG, IgM, lactoferrin, lysozyme, proline-rich proteins, statherin, histatin 1, and mucous glycoprotein 1 were observed. We further confirmed the presence of proline-rich proteins, lactoferrin, lysozyme, and carbonic anhydrase II by probing in vivo pellicle with specific polyclonal anti-sera. The polyclonal antibody approach provided a powerful method for the identification of various pellicle proteins, including some which show mineral homeostasis or antimicrobial activity.
Li
J
J
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, USA.
Helmerhorst
E J
EJ
Troxler
R F
RF
Oppenheim
F G
FG
D000276:Adjuvants, Immunologic; D000681:Amylases / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000097:blood*; D024402:Carbonic Anhydrase II / Q000032:analysis; D044622:Dental Pellicle / Q000737:chemistry* / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D054808:Histatins; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D055257:Mucin-5B; D009077:Mucins / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010748:Phosphopeptides / Q000032:analysis; D011392:Proline / Q000032:analysis; D055232:Proline-Rich Protein Domains; D012471:Salivary Proteins and Peptides / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis
D000276:Adjuvants, Immunologic; D000906:Antibodies; D006023:Glycoproteins; D054808:Histatins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C524578:MUC5B protein, human; D055257:Mucin-5B; D009077:Mucins; D010455:Peptides; D010748:Phosphopeptides; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D012709:Serum Albumin; C068074:HTN1 protein, human; D011392:Proline; D000681:Amylases; D009113:Muramidase; D007781:Lactoferrin; D024402:Carbonic Anhydrase II