title: Identification of a metallothionein in Synechococcus by capillary electrophoresis hyphenated with inductively coupled plasma mass spectrometry.
abstract: A home-made system hyphenating capillary electrophoresis with an inductively coupled plasma mass spectrometer (CE-ICP-MS) for cadmium speciation of protein-binding and free cadmium ions in solution is presented. The CE-ICP-MS interface consisted of an acrylic block with an internal volume ca. 20 microL in which a platinum electrode, a capillary column, and a connection to an ICP nebulizer were inserted. A make-up electrolyte solution containing 50 mmol L(-1) Tris-HCl buffer solution (pH 9.0) was continuously flowed through the interface to the ICP nebulizer. The separation of free Cd ions, Cd-cysteine, and Cd bounded to metallothionein (MT) isoforms from rabbit liver was carried out by capillary electrophoresis, and the analytes were detected by ICP-MS. The feasibility to isolate metallothionein compounds extracted from the cyanobacterium Synechococcus PCC7942 was demonstrated. The Cd binding proteins were induced in Synechococcus PCC7942 and further analyzed by CE ICP-MS.
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário 303, 13416-000, Piracicaba, SP, Brazil.
authors.forename: André F
authors.initials: AF
authors.lastname: Lavorante
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Giné
authors.forename: Ana Paula G
authors.initials: AP
authors.lastname: Gervasio
authors.forename: Carlos E S
authors.initials: CE
authors.lastname: Miranda
authors.forename: Marli F
authors.initials: MF
authors.lastname: Fiore
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Bellato
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
mesh_terms: D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000458:Cyanobacteria / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008668:Metallothionein / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits
chemical_list: D002104:Cadmium; D008668:Metallothionein