[Therapeutic effect of secretive endostatin eukaryotic expressing plasmid on mouse hepatoma].
OBJECTIVE
To construct and express secretive endostatin eukaryotic plasmid for treatment of hepatoma.


METHODS
Mouse Igk signal peptide sequence was synthesized and cloned into pcDNA3.1 with endostatin gene. The supernant of BHK-21 transfected with recombinant was used to culture ECV304. The proliferation of latter was evaluated by MTT assay. H22 was inoculated intramusclely, then naked DNA of endostatin plasmid was injected into the inoculation site. Tumors were dissected and weighted after treatments. All data was analyzed by SPSS10.0.


RESULTS
The supernant of BHK-21 transfected with recombinant can inhibit the proliferation of ECV304 by 29.2%. Tumor weight lighter after injected with naked pSecES (1.34 g+/-0.96g) compared with naked pcDNA3.1 (2.70g+/-0.82g) and saline (3.73g+/-1.41g).


CONCLUSIONS
The endostatin eukaryotic plasmid was constructed and it can be used for gene therapy on hepatoma.
Li
Pei-yuan
PY
Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Lin
Ju-sheng
JS
Feng
Zuo-hua
ZH
Zhang
Hui
H
Zhou
He-jun
HJ
Zhang
Jin-yan
JY
D000818:Animals; D043169:Endostatins / Q000235:genetics* / Q000378:metabolism; D015316:Genetic Therapy; D008114:Liver Neoplasms, Experimental / Q000628:therapy*; D008297:Male; D051379:Mice; D010957:Plasmids
D043169:Endostatins