title: Toll-like receptor gene family and TIR-domain adapters in Danio rerio.
abstract: The toll-like family of receptors (TLR) is an ancient pattern recognition receptor family, conserved from insects to mammals. We have identified in zebrafish (Danio rerio) 19 putative TLR variants, the orthologs of mammalian TLR2-5, 7-9, a fish specific receptor type group and three putative splice variants. One receptor is very close to mammalian TLR1, 6 and 10 and seems to be their common ancestor. However, in contrast to the pufferfish, Fugu rubripes, we found two receptors homologous to TLR4, showing that lack of TLR4 is not general for fish. In addition, we identified two members close to mammalian TLR8 and five members close to FuguTLR21 and goldfish TLR, a TLR group which now has only been found in fish. By RT-PCR we showed that all TLR are widely expressed in adult tissues, but also at different stages of development. All these TLRs contain very conserved toll/interleukin-1 receptor (TIR) domains able to interact with TIR-domain of adapter molecules. We demonstrate here that TIR-domain containing adapters MyD88 and SARM are present in zebrafish, showing that TLR adapter molecules are highly conserved in evolution.
authors.affiliation: Dynamique des Interactions Système Immunitaire et Cancer, Unité INSERM 517, Faculté des Sciences Gabriel, 6, bld Gabriel, F-21800 Dijon, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Jault
authors.forename: Laurent
authors.initials: L
authors.lastname: Pichon
authors.forename: Johanna
authors.initials: J
authors.lastname: Chluba
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051200:Toll-Like Receptor 8; D051193:Toll-Like Receptors; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology
chemical_list: D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051200:Toll-Like Receptor 8; D051193:Toll-Like Receptors