Current applications of flow cytometry in the diagnosis of primary immunodeficiency diseases.
BACKGROUND
To review the applications of flow cytometry in the diagnosis and management of primary immunodeficiency disease.


METHODS
Articles describing the use of flow cytometry in the diagnosis of several primary immunodeficiency diseases were obtained through the National Library of Medicine database.


METHODS
Publications that described novel and known applications of flow cytometry in primary immunodeficiency disease were selected. Review articles were included. Articles describing the different immunodeficiency diseases and methods of diagnosis were also selected.


METHODS
Approximately 100 data sources were analyzed, and those with the most relevant information were selected.


RESULTS
The diagnosis of many primary immunodeficiency diseases requires the use of several laboratory tests. Flow cytometry has become an important part of the workup of individuals suspected to have such a disorder. Knowledge of the pathogenesis of many of these diseases continues to increase, hence we acquire a better understanding of the laboratory tests that may be helpful in diagnosis.


CONCLUSIONS
Flow cytometry is applicable in the initial workup and subsequent management of several primary immunodeficiency diseases. As our understanding of the pathogenesis and management of these diseases increases, the use of many of these assays may become routine in hospitals.
Illoh
Orieji C
OC
Department of Pathology, University of Virginia Health System, Charlottesville 22908, USA. oci8t@virginia.edu
D015703:Antigens, CD / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000175:diagnosis* / Q000276:immunology; D016130:Immunophenotyping; D016897:Respiratory Burst
D015703:Antigens, CD