[Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts].
OBJECTIVE
To study DNA damage of three kinds of gasoline oxygenates.


METHODS
Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts.


RESULTS
In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative.


CONCLUSIONS
Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
Song
Chonglin
C
National Engine Combustion Laboratory, Tianjin University, Tianjin 300072, China.
Zhang
Zhifu
Z
Chen
Xue
X
Zhang
Yanfeng
Y
Wang
Chunhua
C
Liu
Keming
K
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D005561:Formates / Q000633:toxicity*; D008738:Methyl Ethers / Q000633:toxicity*; D051379:Mice
D005561:Formates; D008738:Methyl Ethers; C043243:methyl tert-butyl ether; D000431:Ethanol; C023025:methyl carbonate