title: A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces.
abstract: A new design for a high pressure MALDI-FTMS instrument is described and initial data are shown. The instrument incorporates a large, 10 cm x 10 cm, sample translation stage to accommodate and position the MALDI target. The new instrument allows coupling to a wide variety of surface techniques such as gel electrophoresis or surface plasmon resonance. Coupling to thin layer chromatography is shown. Furthermore, a new nozzle design allows high pressure collisional cooling sufficient to stabilize gangliosides while minimizing the gas load on the system.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA. poconnor@bu.edu
authors.forename: Peter B
authors.initials: PB
authors.lastname: O'Connor
authors.forename: Bogdan A
authors.initials: BA
authors.lastname: Budnik
authors.forename: Vera B
authors.initials: VB
authors.lastname: Ivleva
authors.forename: Parminder
authors.initials: P
authors.lastname: Kaur
authors.forename: Susanne C
authors.initials: SC
authors.lastname: Moyer
authors.forename: Jason L
authors.initials: JL
authors.lastname: Pittman
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
mesh_terms: D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*
chemical_list: D005732:Gangliosides