Roles of SWI/SNF and HATs throughout the dynamic transcription of a yeast glucose-repressible gene.
Eucaryotic gene expression requires chromatin-remodeling activities. We show by time-course studies that transcriptional induction of the yeast glucose-regulated SUC2 gene is rapid and shows a striking biphasic pattern, the first phase of which is partly mediated by the general stress transcription factors Msn2p/Msn4p. The SWI/SNF ATP-dependent chromatin-remodeling complex associates with the promoter in a similar biphasic manner and is essential for both phases of transcription. Two different histone acetyltransferases, Gcn5p and Esa1p, enhance the binding of SWI/SNF to the promoter during early transcription and are required for optimal SUC2 induction. Gcn5p is recruited to SUC2 simultaneously with SWI/SNF, whereas Esa1p associates constitutively with the promoter. This study reveals an unusual transcription pattern of a metabolic gene and suggests a novel strategy by which distinct chromatin remodelers cooperate for the dynamic activation of transcription.
Geng
Fuqiang
F
Program in Molecular and Cellular Biology, Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY, USA.
Laurent
Brehon C
BC
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D016193:G1 Phase; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D051548:Histone Acetyltransferases; D007700:Kinetics; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D043324:beta-Fructofuranosidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D002843:Chromatin; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase