Nodule-enhanced protease inhibitor gene: emerging patterns of gene expression in nodule development on Sesbania rostrata.
A novel marker for the early stages of nodulation of Sesbania rostrata was found to encode a putative member of the Kunitz family of protease inhibitors (SrPI1). Its expression was enhanced during nodulation, and was not up-regulated by wounding or upon infection with wide host-range pathogens. In situ expression patterns resembled those previously described for functions that may be implicated in delimiting infected nodule tissues from the rest of the plant. Thus, SrPI1 may be a component of a multi-layered barrier that restrains the invading rhizobia.
Lievens
Sam
S
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Gent, Belgium.
Goormachtig
Sofie
S
Holsters
Marcelle
M
D000595:Amino Acid Sequence; D020599:Azorhizobium caulinodans / Q000254:growth & development*; D020171:Botrytis / Q000254:growth & development; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013314:Stress, Mechanical; D013559:Symbiosis / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; C053865:Kunitz-type protease inhibitor, plant; D010455:Peptides; D010940:Plant Proteins