Differentiation of mouse embryonic stem cells into pancreatic and hepatic cells.
Here, we present efficient strategies to differentiate ES cells either into pancreatic or into hepatic cell types. We recommend a strategy to select nestin+ cells, an early progenitor cell type with high developmental plasticity, followed by differentiation induction with specific growth and extracellular matrix factors into pancreatic and hepatic cell types. Cells differentiating via nestin+ cells into the pancreatic and hepatic lineage expressed tissue-specific genes. Proteins characteristic for mature endocrine pancreatic or hepatic cells were synthesized and released. Further, a histotypic "spinner" culture system was introduced to generate mature insulin- and albumin-producing cells at high efficiency.
Kania
Gabriela
G
In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, Gatersleben, D-06466, Germany.
Blyszczuk
Przemyslaw
P
Czyz
Jaroslaw
J
Navarrete-Santos
Anne
A
Wobus
Anna M
AM
D000418:Albumins / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009419:Nerve Tissue Proteins; D064231:Nestin; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
D000418:Albumins; D007328:Insulin; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin