Chromosome-protein interactions in polyomavirus virions.
In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.
Carbone
Mariarosaria
M
Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, 00161 Rome, Italy.
Ascione
Giuseppe
G
Chichiarelli
Silvia
S
Garcia
Marie-Isabelle
MI
Eufemi
Margherita
M
Amati
Paolo
P
D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism*; D002945:Cisplatin / Q000378:metabolism; D003432:Cross-Linking Reagents; D051379:Mice; D011120:Polyomavirus / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*; D019065:Virus Assembly
D036022:Capsid Proteins; D003432:Cross-Linking Reagents; D002945:Cisplatin