[Mechanisms of acetylcholine-dependent production of H2O2 and NO2- by stromal cells of endometrium].
The metabolism of NO(NO2-) and H2O2(O2-) by stroma cells of pig endometrium is NAD(P)H and glutathione-dependent process. The efficiency of biosynthesis and utilization of these metabolites appreciably depends on the state of SH-groups of the conforming ferment systems. And the reversible oxidation of SH-groups (maybe by the reaction products) results in the drop of biosynthesis rate. The NO and H2O2 metabolism is also defined by the state of oxidative metabolism of arachidonic acid (depending on salicylate), and also intensity of a course of redox-processes on plasmalemma (is regulated by cytochrome c). The NO2- biosynthesis by stroma cells is strongly inhibited by the agents, which super produce H2O2(O2-) (salicylate and cytochrome c). The NO(NO2-) and H2O2(O2-) metabolism at stimulation by acetylcholine is of cyclic character, and the infringement of any link during biosynthesis or utilization of these compositions results in losses of cyclicity. In contrast to this the formation of nitrosoglutathione with time achieves the saturation, which reflects its buffer and depositing with respect to NO function and permits to consider formation of the latter as one of mechanisms of effective utilization of NO(NO2-)--by the stroma cells.
Danylovych
Iu V
IuV
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv. kinet@biochem.kiev.ua
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism*; D066298:In Vitro Techniques; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism*
D009566:Nitrates; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D000109:Acetylcholine