title: Bcl10 activates the NF-kappaB pathway through ubiquitination of NEMO.
abstract: The NF-kappaB family of transcription factors is activated in response to many stimuli, including pro-inflammatory cytokines, environmental stresses and, in the case of B and T lymphocytes, by antigenic stimulation. Bcl10 is essential for NF-kappaB activation by T- and B-cell receptors. T and B lymphocytes from Bcl10-deficient mice fail to activate NF-kappaB in response to antigen-receptor stimulation and, as a consequence, are unable to proliferate. Bcl10 overexpression is sufficient to activate NF-kappaB, a process that requires the NF-kappaB essential modulator NEMO (also known as IKK-gamma), which is the regulatory subunit of the IkappaB kinase complex. However, the cellular mechanism by which Bcl10 activates the NF-kappaB pathway remains unclear. Here we show that Bcl10 targets NEMO for lysine-63-linked ubiquitination. Notably, a mutant form of NEMO that cannot be ubiquitinated inhibited Bcl10-induced NF-kappaB activation. Paracaspase and a ubiquitin-conjugating enzyme (UBC13) were both required for Bcl10-induced NEMO ubiquitination and subsequent NF-kappaB activation. Furthermore, short interfering RNAs that reduced the expression of paracaspase and UBC13 abrogated the effects of Bcl10. Thus, the adaptor protein Bcl10 promotes activation of NF-kappaB transcription factors through paracaspase- and UBC13-dependent ubiquitination of NEMO.
authors.affiliation: Department of Molecular Oncology, Genentech Inc. 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Honglin
authors.initials: H
authors.lastname: Zhou
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wertz
authors.forename: Karen
authors.initials: K
authors.lastname: O'Rourke
authors.forename: Mark
authors.initials: M
authors.lastname: Ultsch
authors.forename: Somasekar
authors.initials: S
authors.lastname: Seshagiri
authors.forename: Michael
authors.initials: M
authors.lastname: Eby
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Vishva M
authors.initials: VM
authors.lastname: Dixit
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; D020169:Caspases; D002460:Cell Line; D017353:Gene Deletion; D006801:Humans; D051550:I-kappa B Kinase; D019169:Jurkat Cells; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein; D016328:NF-kappa B / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013379:Substrate Specificity; D025801:Ubiquitin / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D016328:NF-kappa B; D009363:Neoplasm Proteins; D025801:Ubiquitin; D044763:Ubiquitin-Conjugating Enzymes; D017346:Protein Serine-Threonine Kinases; C496559:CHUK protein, human; D051550:I-kappa B Kinase; C496560:IKBKB protein, human; C496565:IKBKE protein, human; D020169:Caspases; C400109:MALT1 protein, human; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein