Expression patterns of claudins, tight junction adhesion molecules, in the inner ear.
Tight junctions (TJs) are indispensable for the establishment of compositionally distinct fluid compartments in the inner ear, but our knowledge of the claudins, TJ adhesion molecules, in the inner ear is still fragmentary. We examined the expression and distribution of claudin-1 to claudin-18 (except for claudin-7, -13 and -17) in the inner ear by immunofluorescence microscopy. In the cochlea, the organ of Corti expressed claudin-1, -2, -3, -9, -10, -12, -14 and -18. In the stria vascularis, claudin-1, -2, -3, -8, -9, -10, -12, -14 and -18 were expressed in the marginal cells, whereas the basal cells were positive only for claudin-11. In Reissner's membrane and the spiral limbus, the expression of claudin-1, -2, -3, -8, -9, -10, -12, -14 and -18 was detected. Furthermore, in the vestibule, claudin-1, -3, -9, -12, -14 and -18 were expressed in the sensory epithelia, whereas in the dark cell area claudin-1, -3, -8, -9, -12, -14 and -18 were detectable. These findings, i.e., very complex expression patterns of claudin species in the inner ear, would reflect the importance and the complexity of the barrier function of TJs in the inner ear.
Kitajiri
Shin-iciro
SI
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8501, Japan. htsukita@mfour.med.kyoto-u.ac.jp
Furuse
Mikio
M
Morita
Kazumasa
K
Saishin-Kiuchi
Yumiko
Y
Kido
Hirofumi
H
Ito
Juichi
J
Tsukita
Shoichiro
S
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D007758:Ear, Inner / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D019108:Tight Junctions / Q000378:metabolism; D014018:Tissue Distribution
D015815:Cell Adhesion Molecules; D008565:Membrane Proteins