Effects of rhinovirus infection on the adherence of Streptococcus pneumoniae to cultured human airway epithelial cells.
To examine the effects of rhinovirus (RV) infection on the adherence of Streptococcus pneumoniae to human tracheal epithelial cells, cells were infected with RV-14, and S. pneumoniae were added to the culture medium. The number of S. pneumoniae adhering to epithelial cells increased after RV infection. Y-24180, a specific inhibitor of the platelet-activating factor receptor (PAF-R); PAF; and the pyrrolidine derivative of dithiocarbamate, an inhibitor of transcription factor nuclear factor-kappaB (NF-kappaB), decreased the number of S. pneumoniae adhering to cells after RV-14 infection. RV-14 infection increased PAF-R expression and the activation of NF-kappaB and promoter-specific transcription factor 1. These findings suggest that RV-14 infection stimulates S. pneumoniae adhesion to airway epithelial cells via increases in PAF-Rs that are partly mediated through activation of transcription factors. Increased adherence of S. pneumoniae may be one of the reasons that pneumonia develops after RV infection.
Ishizuka
Satoshi
S
Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai, Japan.
Yamaya
Mutsuo
M
Suzuki
Tomoko
T
Takahashi
Hidenori
H
Ida
Shiroh
S
Sasaki
Takahiko
T
Inoue
Daisuke
D
Sekizawa
Kiyohisa
K
Nishimura
Hidekazu
H
Sasaki
Hidetada
H
D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology; D010850:Picornaviridae Infections; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D011008:Pneumococcal Infections / Q000382:microbiology*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism / Q000382:microbiology; D012229:Rhinovirus / Q000472:pathogenicity*; D013296:Streptococcus pneumoniae / Q000472:pathogenicity / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology; D014774:Virulence
D016328:NF-kappa B; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D014157:Transcription Factors; C039351:platelet activating factor receptor