SREBP contributes to induction of collagen VI transcription by serum starvation.
Collagen VI is a main extracellular matrix protein whose mutation is linked to myopathic diseases. In myoblasts and other cell types, collagen VI gene transcription peaks during cell-cycle exit that precedes differentiation, upon serum withdrawal or confluence. To get insight into this transcriptional regulation, we characterized a growth arrest responsive region (GARR) in the Col6a1 promoter responsible for this effect. In this work, we identify sterol regulatory element binding protein (SREBP) as a GARR binding protein and provide evidence that SREBP contributes to induction of Col6a1 transcription in serum free conditions. Furthermore, our data unveil a previously unexpected link between extracellular matrix production and LDL signaling.
Ferrari
Alessandra
A
Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padua 35121, Italy.
Maretto
Silvia
S
Girotto
Davide
D
Volpin
Dino
D
Bressan
Giorgio M
GM
D000818:Animals; D015152:Blotting, Northern; D022762:CCAAT-Enhancer-Binding Proteins / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D024142:Collagen Type VI / Q000096:biosynthesis* / Q000235:genetics; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D015723:Gene Library; D005982:Glutathione Transferase / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D032342:Muscle Cells / Q000378:metabolism; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
D022762:CCAAT-Enhancer-Binding Proteins; D024142:Collagen Type VI; D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008077:Lipoproteins, LDL; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D012313:RNA; D005982:Glutathione Transferase