Newly identified exons encoding novel variants of p94/calpain 3 are expressed ubiquitously and overlap the alpha-glucosidase C gene.
There are two classes of an intracellular 'modulator protease', calpain: ubiquitous and tissue-specific. p94/calpain 3 is an example of the latter, predominantly expressed in muscle. A defect in the p94 gene causes muscular dystrophy. Here we report that human and mouse p94 genes have a possible novel alternative promoter expressing p94 variants in all tissues examined including human lens epithelial cells. The possible promoter region and the following novel exons overlap the 3' region of the neutral alpha-glucosidase C gene. Unlike p94, the novel p94 variants expressed in COS7 cells do not undergo rapid autolysis, suggesting basic functions different from p94.
Kawabata
Yukiko
Y
Laboratory of Biological Function, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hata
Shoji
S
Ono
Yasuko
Y
Ito
Yoshimasa
Y
Suzuki
Koichi
K
Abe
Keiko
K
Sorimachi
Hiroyuki
H
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002154:Calpain / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005091:Exons / Q000235:genetics*; D020869:Gene Expression Profiling; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D051381:Rats; D000520:alpha-Glucosidases / Q000235:genetics*
D017931:DNA Primers; D020033:Protein Isoforms; D000520:alpha-Glucosidases; D002154:Calpain; C078134:calpain p94