Heat and cold-induced male sterility in Drosophila buzzatii: genetic variation among populations for the duration of sterility.
Here we studied three phenotypic traits in Drosophila buzzatii that are strongly effected by temperature, and are expected to be closely associated with fitness in nature. The traits measured were thermal threshold of male sterility, time for males to gain fertility when reared at a sterility-inducing temperature and transferred to 25 degrees C on eclosion and survival after development. The last two traits were measured under four temperature regimes, constant 12 degrees C, 25 degrees C, 31 degrees C, and fluctuating 25 degrees C (18 h) and 38 degrees C (6 h). We looked for genetic variation in these traits and relations among them in four lines of D. buzzatii originating from Argentina and Tenerife. The thermal threshold of heat-induced male sterility was found to lie within the range of 30.0-31.0 degrees C. When measuring the time for males to gain fertility, males reared at a nonstressful temperature (25 degrees C) were fertile 58-67 h after emergence with only minor differences among lines. When reared constant 31 degrees C, males were fertile 174-225 h after hatching. The Argentinean lines were significantly faster in recovering from sterility than were the lines from Tenerife. When reared in a fluctuating temperature regime, differences among lines increased, dividing the lines into three significantly different groups, with a sterility period of 135-215 h. When reared at 12 degrees C from the pupal stage, males were fertile after 106-130 h with significant difference in the variance but not in the mean duration of sterility. Significant differences in viability were found among development temperatures, but not among lines, and viability and the duration of sterility seem to be genetically independent.
Vollmer
J H
JH
Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, 8000 Aarhus C, Denmark.
Sarup
P
P
Kaersgaard
C W
CW
Dahlgaard
J
J
Loeschcke
V
V
D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D003080:Cold Temperature; D004330:Drosophila / Q000502:physiology*; D005298:Fertility / Q000502:physiology*; D014644:Genetic Variation; D006358:Hot Temperature; D008297:Male; D011153:Population / Q000235:genetics