Induction of PYPAF1 during in vitro maturation of mouse mast cells.
Coculture of mouse bone marrow-derived immature mast cells (BMMC) with Swiss 3T3 fibroblasts in the presence of stem cell factor (SCF) promotes morphological and functional maturation toward a connective tissue mast cell (CTMC)-like phenotype, which is accompanied by increased expression of several unique genes. Here we report the molecular identification of one of them, mast cell maturation-associated inducible gene (MMIG)-1. The MMIG-1 cDNA encodes a 117-kDa cytosolic protein that comprises an N-terminal PYRIN domain, a central nucleotide-binding domain, and nine C-terminal leucine-rich repeats. MMIG-1 shows >85% sequence similarity to human cryopyrin/PYPAF1, a causal gene for familial cold urticaria and Muckle-Wells syndrome. MMIG-1 was distributed in the cytosol of CTMC-like differentiated BMMC. MMIG-1 underwent alternative splicing in the leucine-rich repeats and each variant was induced differently in BMMC during coculture. Moreover, its expression was increased in the ears of mice with experimental atopic dermatitis. Thus, MMIG-1, a likely mouse PYPAF1 ortholog, may play a role in mast cell-directed inflammatory diseases.
Kikuchi-Yanoshita
Rei
R
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555.
Taketomi
Yoshitaka
Y
Koga
Kumiko
K
Sugiki
Toshihiko
T
Atsumi
Yohei
Y
Saito
Takanori
T
Ishii
Shin-Ichi
S
Hisada
Masato
M
Suzuki-Nishimura
Tamiko
T
Uchida
Masaatsu K
MK
Moon
Tae-Chul
TC
Chang
Hyeun-Wook
HW
Sawada
Masatsugu
M
Inagaki
Naoki
N
Nagai
Hiroichi
H
Murakami
Makoto
M
Kudo
Ichiro
I
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D003001:Cloning, Molecular; D018920:Coculture Techniques; D018076:DNA, Complementary / Q000235:genetics; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000235:genetics; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
D002352:Carrier Proteins; D018076:DNA, Complementary; D004139:Dinitrofluorobenzene; D012333:RNA, Messenger; C492716:Nlrp3 protein, mouse; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein