A structural study of the retinal photoreceptor, plexiform and ganglion cell layers following exposure to UV-B and UV-C radiation in the albino rat.
Over the last two decades, ultraviolet radiation levels (UV), reaching the Earth's surface, have been increasing at a rate of 1.5% per each 1% loss of the ozone layer. Moreover, artificial UV-sources have also proliferated and contributed to the rising UV-stress that many organisms have to face. To assess how the vertebrate retina responds to an exposure of short wavelength UV, we focused our attention on the rat retina, observing photoreceptor (containing outer and inner segments of rods and cones), inner plexiform, and ganglion cell layers by light and transmission electron microscopy using conventional and cytochemical techniques. We analyzed how cells of the layers in question responded to a 30 min exposure to UV-C and UV-B radiation with doses of 7200 and 590 J/cm(2), respectively. The results show that there are significant changes in the nuclei and cytoplasmic organelles of the exposed retinae when compared with those of the unexposed controls. The changes include an increase in heterochromatin, distension of rough endoplasmic reticulum, mitochondrial disruptions, and increases in the number of myelin bodies. The recorded morphological changes, especially those of the ganglion cells, are suggestive of apoptotic processes and show that the exposure of vertebrate retina to wavelengths ranging from 254 to 312 nm can produce alterations that are likely to impact negatively on the retina's proper functioning.
de Oliveira Miguel
Nadia Campos
NC
Departamento de Histologia e Embriologia, Centro de Ciencias da Saude (CCS), Instituto de Ciencias Biomedicas (ICB), Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brazil.
Meyer-Rochow
Victor Benno
VB
Allodi
Silvana
S
D000818:Animals; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D010786:Photoreceptor Cells / Q000528:radiation effects / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000528:radiation effects* / Q000648:ultrastructure*; D012165:Retinal Ganglion Cells / Q000528:radiation effects / Q000648:ultrastructure; D014466:Ultraviolet Rays / Q000009:adverse effects*