title: Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: a real time scanning tunnelling microscopy study.
abstract: A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C, which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in development of a bio-electrode surface has been predicted.
authors.affiliation: New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QT, UK. rupam@iitk.ac.in
authors.forename: R
authors.initials: R
authors.lastname: Mukhopadhyay
authors.forename: K K
authors.initials: KK
authors.lastname: Lo
authors.forename: L L
authors.initials: LL
authors.lastname: Wong
authors.forename: H A O
authors.initials: HA
authors.lastname: Hill
mesh_terms: D000327:Adsorption; D002021:Buffers; D004800:Enzymes, Immobilized; D005288:Ferredoxins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D002021:Buffers; D004800:Enzymes, Immobilized; D005288:Ferredoxins; C100137:putidaredoxin; D006046:Gold