title: [A study on the relationship between urinary 1-hydroxypyrene level and early genetic effect among coke oven workers].
abstract: OBJECTIVE
  To investigate the relationship between the urinary 1-hydroxypyrene level and cytokinesis-block micronucleus and the olive moment of comet assay in peripheral blood lymphocyte in coke oven workers.
  
  
  METHODS
  One hundred and thirty-three workers from a coke plant and 28 referents without occupational PAH exposure were recruited in this study. Urinary level of 1-hydroxypyrene was measured by alkaline hydrolysis combined with high performance liquid chromatography as an internal exposure dose, and the DNA and chromosomal damage of peripheral blood lymphocyte were evaluated with comet assay and cytokinesis-block micronucleus method. Personal information including occupational history, age, sex, smoking and alcohol drinking, was collected by questionnaire.
  
  
  RESULTS
  There existed a good correlationship between the urinary level of 1-hydroxypyrene and frequency of micronuclei per 1 000 binucleated cells or the olive moment of comet assay in the study subjects, after adjusting for sex, age, smoking and alcohol drinking (r > 0.25, P < 0.01). One hundred and sixty-one subjects were divided into three groups by their urine 1-hydroxypyrene level (expressed as 0.30 - 2.44, 2.45 - 7.09 and 7.10 - 33.10 micro mol/mol Cr), and the geometric means of their urinary levels of 1-hydroxypyrene were 1.14, 4.32 and 12.49 micro mol/mol Cr, respectively. After adjusting for age, sex, smoking and alcohol drinking by multiple nonparametric analysis of covariance, the median of olive moment of comet assay in the group of 7.10 - 33.10 micro mol/mol Cr was 3.67, significantly higher than that in the groups of 0.30 - 2.44 and 2.45 - 7.09; and the micronuclei frequencies in the groups of 2.45 - 7.09 and 7.10 - 33.10 micro mol/mol Cr were 8.00 per thousand and 7.50 per thousand, respectively, significantly higher than that in the group of 0.30 - 2.44 micro mol/mol Cr (6.00 per thousand ).
  
  
  CONCLUSIONS
  The comet assay of peripheral blood lymphocyte was more suitable to detect the PAHs-induced early genotoxicity, than the cytokinesis-block micronucleus.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Leng
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zheng
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Wen-Zhong
authors.initials: WZ
authors.lastname: Zhang
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Li
authors.forename: Zu-Fei
authors.initials: ZF
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Zhong-Xu
authors.initials: ZX
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Feng-Sheng
authors.initials: FS
authors.lastname: He
mesh_terms: D000328:Adult; D003077:Coke / Q000009:adverse effects*; D020552:Comet Assay; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000009:adverse effects*; D011721:Pyrenes / Q000378:metabolism*
chemical_list: D003077:Coke; D011721:Pyrenes; C033146:1-hydroxypyrene