title: Responses of cultured human airway epithelial cells treated with diesel exhaust extracts will vary with the engine load.
abstract: Epidemiologic evidence suggests that increased morbidity and mortality are associated with the concentrations of ambient air particulate matter (PM). Many sources contribute to the particulate fraction of ambient air pollution, including diesel exhaust particulates (DEP). Diesel exhaust also contributes gas-phase pollutants to the atmosphere, and gaseous copollutants may influence the toxicity of PM. The composition of diesel exhaust varies greatly depending on the engine load conditions as well as other factors. To determine whether different diesel exhaust composition can affect lung cell resposes, the effects of of diesel exhaust extracts derived from different engine loads were examined on normal human bronchial epithelial cells (NHBE) in vitro. Diesel exhaust was collected into chilled impingers containing phosphate-buffered saline (PBS). Cultured NHBE cells were treated with 0 to 500 microg/well extract from approximately 0% engine load (termed low load or LL) or extract from approximately 75% engine load (termed high load or HL) for 24 h. The HL extract was cytotoxic at 500 microg compared to controls as measured by (51)Cr release. Production of the neutrophil chemotaxin interleukin 8 (IL-8) was decreased 4.7-fold in cells treated with 500 microg LL extract, whereas cells treated with 500 microg HL extract showed a 2.4-fold increase in IL-8 release. Production of the inflammatory and immune system mediator prostaglandin E(2) (PGE(2)) was increased up to 2.5-fold in cells treated with HL extract, but unchanged with other treatments. Melittin stimulation of cells showed that the LL extract had an inhibitory effect on PGE(2) release at 500 microg. Differences in carbonyl content of the extracts were found by high performance liquid chromatography/mass spectroscopy HPLC/MS, with the HL extract having more intermediate size carbonyls (i.e. with six to nine carbons). The data suggest that the response of NHBE cells to treatment with diesel exhaust will vary depending on the constituent components of the exhaust.
authors.affiliation: National Health and Environmmental Effects Research Laboratory, ORD, U.S. EPA, Research Triangle Park, NC, USA. madden.michael@epa.gov
authors.forename: Michael C
authors.initials: MC
authors.lastname: Madden
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Dailey
authors.forename: Jacqueline G
authors.initials: JG
authors.lastname: Stonehuerner
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Harris
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D000397:Air Pollution; D001980:Bronchi / Q000166:cytology / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D012140:Respiratory Tract Diseases / Q000139:chemically induced*; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D001335:Vehicle Emissions