title: Immobilization of phospholipase A2 on porous glass and its application for lowering serum cholesterol concentration.
abstract: Phospholipase A2 (PLA2; EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA2 from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50 degrees C, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey. mustafateke@mail.ege.edu.tr
authors.forename: Mustafa
authors.initials: M
authors.lastname: Teke
authors.forename: Seçil
authors.initials: S
authors.lastname: Onal
authors.forename: Ali
authors.initials: A
authors.lastname: Kilinç
authors.forename: Azmi
authors.initials: A
authors.lastname: Telefoncu
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood* / Q000302:isolation & purification; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000627:therapeutic use; D005898:Glass; D006863:Hydrogen-Ion Concentration; D006937:Hypercholesterolemia / Q000628:therapy; D008954:Models, Biological; D010741:Phospholipases A / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D054467:Phospholipases A2; D013552:Swine; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D002784:Cholesterol; D010741:Phospholipases A; D054467:Phospholipases A2