title: Shifting foci of hematopoiesis during reconstitution from single stem cells.
abstract: To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the spleen and bone marrow (BM), at a frequency that correlated with BM compartment size. Initially detected foci could expand locally, seed other sites in BM or spleen, and/or recede with different kinetics. These studies reveal dynamic and variable patterns of engraftment from highly purified HSC and indicate that the final overall contribution of individual HSC to hematopoietic chimerism does not depend on the specific site of initial engraftment and expansion.
authors.affiliation: Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Cao
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Wagers
authors.forename: Andreas
authors.initials: A
authors.lastname: Beilhack
authors.forename: Joan
authors.initials: J
authors.lastname: Dusich
authors.forename: Michael H
authors.initials: MH
authors.lastname: Bachmann
authors.forename: Robert S
authors.initials: RS
authors.lastname: Negrin
authors.forename: Irving L
authors.initials: IL
authors.lastname: Weissman
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Contag
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002678:Chimera; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases