title: A simple parafilm M-based method for blood-feeding Aedes aegypti and Aedes albopictus (Diptera: Culicidae).
abstract: A simple device for blood-feeding Aedes (Stegomyia) mosquitoes is described. Parafilm M is stretched and pressed into fiberglass window screen to form a packet for holding warmed blood. The method has been used successfully to maintain Aedes albopictus colonies for >2 yr. In typical blood-feeding trials, 53 +/- 0.8% (+/-SE) of Aedes aegypti (Linnaeus) and 31 +/- 6% of Ae. albopictus (Skuse) fed to repletion.
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN 47405, USA. mictseng@bio.indiana.edu
authors.forename: Michelle
authors.initials: M
authors.lastname: Tseng
mesh_terms: D000330:Aedes / Q000145:classification* / Q000469:parasitology / Q000502:physiology*; D000818:Animals; D001695:Biology / Q000295:instrumentation / Q000379:methods*; D005247:Feeding Behavior; D012756:Sheep / Q000097:blood*