Cloning, characterization and comparative analysis of pig plasma apolipoprotein A-IV.
Pig apolipoprotein (apo) A-IV cDNA was cloned, characterized and compared to the human ortholog. Mature porcine apo A-IV consists of 362 amino acids and displays a 75.6% sequence identity with human protein. Pig apo A-IV is the smallest reported mammalian apo A-IV because it lacks the repeated motifs of glutamine and glutamic acid at the carboxyl terminus. A phylogenic tree of apo A-IV mammalian proteins reveals that porcine apo A-IV is more closely related to humans and primates than to rodents. This protein is highly hydrophobic and is mainly associated with lipoproteins.
Navarro
María A
MA
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, E-50013 Zaragozza, Spain.
Acín
Sergio
S
Iturralde
María
M
Calleja
Lucía
L
Carnicer
Ricardo
R
Guzmán-García
Mario A
MA
González-Ramón
Nieves
N
Mata
Pedro
P
Isabel
Beatriz
B
López-Bote
Clemente J
CJ
Lampreave
Fermín
F
Piñeiro
Andrés
A
Osada
Jesús
J
D000595:Amino Acid Sequence; D000818:Animals; D001054:Apolipoproteins A / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000235:genetics*
D001054:Apolipoproteins A; D018076:DNA, Complementary; C022205:apolipoprotein A-IV