Validation and application of a liquid chromatography-tandem mass spectrometric method for the determination of SCH 211803 in rat and monkey plasma using automated 96-well protein precipitation.
A rapid, sensitive, specific, accurate, and reproducible automated liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the quantitative determination of 1'-(2-amino-3-methylbenzoyl)-4-[[[(3-chlorophenyl)sulfonyl]phenyl]methyl]-1,4'-bipiperidine hydrochloride (SCH 211803) in plasma has been developed. The method was validated in rat and monkey plasma over the concentration range of 0.5-250 ng/ml using 2H(4)-SCH 211803 as the internal standard (IS). Automated 96-well plate protein precipitation (PP) with acetonitrile (ACN) was used for sample processing. The method employed a Betasil C18 column with a fast gradient for the separation of analyte and internal standard from the plasma matrix and a triple quadrupole mass spectrometer operated in positive ion multiple reaction monitoring (MRM) mode for detection. The method was used for the determination of SCH 211803 plasma concentrations to support pre-clinical studies.
Yang
Liyu
L
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033-1300, USA.
Wu
Ning
N
Clement
Robert P
RP
Rudewicz
Patrick J
PJ
D000818:Animals; D001331:Automation; D005260:Female; D000882:Haplorhini; D018727:Muscarinic Antagonists / Q000097:blood*; D010880:Piperidines / Q000097:blood*; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C483563:1'-(2-amino-3-methylbenzoyl)-4-(((3-chlorophenyl)sulfonyl)phenyl)methyl-1,4'-bipiperidine hydrochloride; D018727:Muscarinic Antagonists; D010880:Piperidines; D011506:Proteins