Novel chiral phosphoramidite monomers from (R)- and (S)-1,3-butanediol: synthesis of modified oligonucleotides and binding affinity studies.
Two optically active phosphoramidite monomers for modified oligodeoxyribonucleotides were prepared. These monomers were then introduced into dodecanucleotides in the middle of the sequences. The modified dodecanucleotides were characterized by various analytical methods including MALDI-TOF mass spectrometry and Tm values were obtained to appraise the binding affinity, by measuring change in UV absorbance at 260 nm.
Kim
Su Jeong
SJ
National Research Lab, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Korea.
Kim
Byeang Hyean
BH
D002072:Butylene Glycols / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry*; D013237:Stereoisomerism
D002072:Butylene Glycols; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C028491:1,3-butylene glycol