The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA.
The nuclear pore protein Nup153 is important for the transport of protein and RNA between the nucleus and cytoplasm. Recently, a novel RNA binding domain (RBD) was mapped within the N-terminal region of Nup153; however, the determinants of RNA association were not characterized. Here we have tested a range of RNAs with different general features to better understand targets recognized by this domain. We have found that the RBD associates with single-stranded RNA with little sequence preference. These results provide new information about a novel RNA binding domain and suggest new models to consider for the contribution of Nup153 to nucleocytoplasmic transport.
Ball
Jennifer R
JR
Department of Oncological Sciences, Huntsman Cancer Institute, The University of Utah, Salt Lake City, Utah 84112, USA.
Dimaano
Christian
C
Ullman
Katharine S
KS
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D017124:Conserved Sequence; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
D028861:Nuclear Pore Complex Proteins; D016601:RNA-Binding Proteins; D012313:RNA