title: [Interrelation between thrombin structure and its stability].
abstract: Data concerning peculiarities of fermentative nature and structure of thrombin in water-salt solution have been generalized; regularities of stabilizing effect made on thrombin by various polyols and other substances have been analyzed. It has been shown that formation of thrombin optimum macrostructure is one of the methods of its stabilization. Presence of different dissolving additives changes this enzymes hydration and this affects its stability and activity. There exist some systems to stabilize thrombin solutions. The systems consist of various salts, low-molecular and high-molecular polyols, surfactants, protein chain, composition buffer, etc. It has been shown that optimal concentrations of polyols, buffer salts and surfactants, as well as protein interaction increase considerably thrombin stability, preserving secondary structure even under its low concentration in the solution.
authors.affiliation: Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv.
authors.forename: M V
authors.initials: MV
authors.lastname: Kolodzeĭskaia
authors.forename: G L
authors.initials: GL
authors.lastname: Volkov
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D004356:Drug Storage; D004795:Enzyme Stability; D006801:Humans; D013379:Substrate Specificity; D013917:Thrombin / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D013917:Thrombin