[Changes of phosphorylation states of Cx43 protein in CCL-64 cells induced by SiO2].
OBJECTIVE
To investigate whether the cellular gap junctional communication(GJIC) down-regulation in alveolar epithelial cells (CCL-64 cells) induced by silica-stimulated pulmonary alveolar macrophages (PAM) supernatant is related with the phosphorylation states of connexin 43(Cx43) protein.


METHODS
Western-blot analysis was used to identify phosphorylated Cx43 species.


RESULTS
Western-blot analyses of SiO2- and phorbol 12-myristate 13-acetate(TPA)-treated CCL-64 cells showed the same phosphorylation states of Cx43 as the control group. There were no Cx43 protein in nucleus of CCL-64 cells.


CONCLUSIONS
The inhibition on GJIC induced by SiO2 and TPA in CCL-64 cells may not be brought about by altering the phosphorylation states of Cx43.
Wu
Weiwei
W
Women's Hospital, Zhejiang University, School of Medicine, Hangzhou 310006, China.
Mao
Guogen
G
D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D018031:Connexin 43 / Q000378:metabolism*; D017629:Gap Junctions / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008907:Mink; D010766:Phosphorylation; D012822:Silicon Dioxide / Q000633:toxicity*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018031:Connexin 43; D012822:Silicon Dioxide; D013755:Tetradecanoylphorbol Acetate