Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling.
OBJECTIVE
Decellularization of aortic valve allografts in advance of transplantation is a promising approach to overcome immune-induced early graft failure. In this study the effects of in vitro cell extraction on extracellular matrix molecules and in vivo remodeling of decellularized aortic valves were investigated in a heterotopic aortic valve rat implantation model.


METHODS
Rat aortic valve conduits were decellularized by a 2-step detergent-enzymatic extraction method involving sodium dodecyl sulfate in combination with RNase and DNase. Cellular and acellular allogeneic (2x, n = 4) and syngeneic valve grafts (2x, n = 3) were grafted infrarenally into the descending aorta for 21 days. Immunohistochemical techniques were used to study extracellular matrix constitution (elastin, collagen, fibronectin, and chondroitin sulfate) and cellular infiltration.


RESULTS
The decellularization procedure resulted in a complete loss of all cellular structures from the entire valve conduit with minimal damage to the extracellular matrix. All transplanted cellular allografts became deformed, swollen, and acellular with major changes in extracellular matrix structure. The transplanted decellularized allografts, however, retained normal preserved valve leaflets comparable to transplanted cellular and acellular syngeneic grafts. With the exception of cellular syngeneic grafts, all other grafts showed retrovalvular thrombi.


CONCLUSIONS
Damage to the valves caused by decellularization technique is much less than the damage caused by the recipient's immune response. In vitro removal of viable cells in (cryopreserved) homografts may decrease graft failure. Seeding with autologous or major histocompatibility complex-matched donor endothelial cells will be necessary to diminish damage induced by an absent blood-tissue barrier.
Grauss
R W
RW
Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands.
Hazekamp
M G
MG
van Vliet
S
S
Gittenberger-de Groot
A C
AC
DeRuiter
M C
MC
D000818:Animals; D001021:Aortic Valve / Q000166:cytology / Q000637:transplantation*; D003902:Detergents; D005109:Extracellular Matrix / Q000737:chemistry; D005260:Female; D008297:Male; D017830:Octoxynol; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014357:Trypsin
D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D014357:Trypsin