title: Iron metabolism in mynah birds (Gracula religiosa) resembles human hereditary haemochromatosis.
abstract: Iron overload is a very frequent finding in several animal species and a genetic predisposition is suggested. In one of the most commonly reported species with susceptibility for iron overload (mynah bird), it was recently shown that the cause of this pathophysiology is high uptake and retention of dietary iron. Here we compare susceptible (mynahs) with non-susceptible avian species (chickens) by evaluating iron uptake at the intestinal absorptive cell level. Enterocytes from mynahs and chickens were isolated and uptake of Fe(II) and Fe(III) was studied in vitro. It was found that Fe(III) uptake is much lower than Fe(II) uptake for both species. Although liver iron, present only in hepatocytes, was at least 10-fold higher in mynahs than chickens, enterocyte Fe(II) uptake was considerably higher in mynahs. Fe(II) uptake showed saturation at the studied concentrations in both species. Kinetic studies revealed a three-fold increase in Vmax for mynahs. Calculated values for the uptake kinetics of the probable membrane transporter suggest that mynah bird enterocytes have a significantly higher limiting uptake rate, due to the possible increase in the number of transporters when compared with chicken enterocytes. The susceptibility of this species is due to intestinal iron uptake despite hepatic iron accumulation, implicating a 'mis-sensing' of body iron similarly to human hereditary haemochromatosis.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands. a.mete@vet.uu.nl
authors.forename: A
authors.initials: A
authors.lastname: Mete
authors.forename: H G
authors.initials: HG
authors.lastname: Hendriks
authors.forename: P H M
authors.initials: PH
authors.lastname: Klaren
authors.forename: G M
authors.initials: GM
authors.lastname: Dorrestein
authors.forename: J E
authors.initials: JE
authors.lastname: van Dijk
authors.forename: J J M
authors.initials: JJ
authors.lastname: Marx
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000235:genetics / Q000378:metabolism*; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D020895:Enterocytes / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006432:Hemochromatosis / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D019190:Iron Overload / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D019266:Iron, Dietary / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D020308:Songbirds / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D019266:Iron, Dietary; D007501:Iron