Group IB phospholipase A2 from Pseudonaja textilis.
Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they showed procoagulant property. cDNA cloning and characterization indicated the presence of two isoforms of PLA(2) proteins in a single snake, each containing the "pancreatic loop," characteristic of group IB phospholipase A(2). The genomic cloning also confirmed the presence of two genes each containing four exons that are interrupted by three introns. Phylogenetic analysis showed that the venom group IB PLA(2) gene is primitive and could have evolved from the same ancestor as the mammalian and venom group IA PLA(2) genes. In the present study, we report that the Pt-PLA2 gene could be responsible for the production of PL1, 2, and 3 possibly via RNA editing process.
Armugam
Arunmozhiarasi
A
Department of Biochemistry, Faculty of Medicine, National University of Singapore 8 Medical Drive, Singapore 117597, Singapore.
Gong
NanLing
N
Li
XiaoJie
X
Siew
Phui Yee
PY
Chai
Siaw Ching
SC
Nair
Ramkishen
R
Jeyaseelan
Kandiah
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001777:Blood Coagulation / Q000187:drug effects; D002384:Catalysis; D002942:Circular Dichroism; D004546:Elapid Venoms / Q000201:enzymology*; D017815:Elapidae; D005796:Genes; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D010802:Phylogeny; D021122:Protein Subunits; D011517:Prothrombin Time; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013918:Thrombin Time
D004546:Elapid Venoms; D007527:Isoenzymes; D021122:Protein Subunits; D010741:Phospholipases A; D054467:Phospholipases A2