Development of a high-throughput screen for protein catalysts: application to the directed evolution of antibody aldolases.
Gildersleeve
Jeff
J
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Varvak
Alex
A
Atwell
Shane
S
Evans
Doug
D
Schultz
Peter G
PG
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D022801:Complementarity Determining Regions; D019020:Directed Molecular Evolution; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011506:Proteins / Q000737:chemistry*
D017151:Antibodies, Catalytic; D022801:Complementarity Determining Regions; D011506:Proteins; D000446:Aldehyde-Lyases