Detection and characterization of cellular immune responses using peptide-MHC microarrays.
The detection and characterization of antigen-specific T cell populations is critical for understanding the development and physiology of the immune system and its responses in health and disease. We have developed and tested a method that uses arrays of peptide-MHC complexes for the rapid identification, isolation, activation, and characterization of multiple antigen-specific populations of T cells. CD4(+) or CD8(+) lymphocytes can be captured in accordance with their ligand specificity using an array of peptide-MHC complexes printed on a film-coated glass surface. We have characterized the specificity and sensitivity of a peptide-MHC array using labeled lymphocytes from T cell receptor transgenic mice. In addition, we were able to use the array to detect a rare population of antigen-specific T cells following vaccination of a normal mouse. This approach should be useful for epitope discovery, as well as for characterization and analysis of multiple epitope-specific T cell populations during immune responses associated with viral and bacterial infection, cancer, autoimmunity, and vaccination.
Soen
Yoav
Y
Department of Biochemistry, Stanford University, Stanford, California, USA.
Chen
Daniel S
DS
Kraft
Daniel L
DL
Davis
Mark M
MM
Brown
Patrick O
PO
D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D019496:Cancer Vaccines / Q000737:chemistry; D002469:Cell Separation; D000939:Epitopes / Q000737:chemistry; D005434:Flow Cytometry; D005805:Genes, MHC Class I / Q000235:genetics; D006801:Humans; D007107:Immune System / Q000378:metabolism*; D007162:Immunoproteins / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010047:Ovalbumin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology
D019496:Cancer Vaccines; D000939:Epitopes; D007162:Immunoproteins; D008024:Ligands; D010455:Peptides; D010047:Ovalbumin; D002118:Calcium