title: Miniaturized pH biosensors based on electrochemically modified electrodes with biocompatible polymers.
abstract: Potentiometric pH sensors based on linear polyethylenimine (L-PEI) and linear polypropylenimine(L-PPI), two synthetic enzymes and biocompatible polymers, films were prepared by electropolymerization of three different monomers: ethylenediamine (EDA), 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA) in order to be used in clinical, dermatological and biological applications, such as in vivo analysis. In a first step a biosensor was tested which consisted in a platinum wire protruded from glass sheath. The polymer film coated on these platinum electrodes showed good linear potentiometric responses to pH changes from pH 3 to 10. Resulting electrodes present both good reversibility and good stability versus time. The effect of the different polymer film thicknesses to potentiometric responses was also studied. This study allowed us to develop a miniaturized pH biosensor in the second step. This sensor was fabricated using photo-lithography, followed by sputtering and lift-off processes, and it included an electronic detection system. We have also successfully studied the potentiometric responses to pH changes of this device over a period of 1 month, and so we propose this new pH micro-biosensor as an alternative to classical pH sensors currently used in dermatology.
authors.affiliation: LCMI, UFR Sciences, Université de Franche-Comté, 16 route de Gray, Besançon 25030, France. boris.lakard@univ-fcomte.fr
authors.forename: Boris
authors.initials: B
authors.lastname: Lakard
authors.forename: Guillaume
authors.initials: G
authors.lastname: Herlem
authors.forename: Michel
authors.initials: M
authors.lastname: de Labachelerie
authors.forename: William
authors.initials: W
authors.lastname: Daniau
authors.forename: Gilles
authors.initials: G
authors.lastname: Martin
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Jeannot
authors.forename: Laurent
authors.initials: L
authors.lastname: Robert
authors.forename: Bernard
authors.initials: B
authors.lastname: Fahys
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D008904:Miniaturization; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011126:Polypropylenes; C443641:poly(propyleneimine); D011094:Polyethyleneimine