Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.
The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).
Castang
Sandra
S
Composante INSA de l'Unité de Microbiologie et Génétique, UMR 5122 CNRS-UCBL-INSA, Bâtiment A. Lwoff, 10 Rue R. Dubois, 69622 Villeurbanne, France.
Shevchik
Vladimir E
VE
Hugouvieux-Cotte-Pattat
Nicole
N
Legrand
Pierre
P
Haser
Richard
R
Gouet
Patrice
P
D003460:Crystallization; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016974:Dickeya chrysanthemi / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006046:Gold / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D006046:Gold; D011133:Polysaccharide-Lyases; C023911:pectate lyase