Induction of T cell anergy by the treatment with IL-10-treated dendritic cells.
In order to apply for reducing graft versus host disease in allogeneic stem cell transplantation, the study concerning the induction of specific T cell anergy was designed. Normal allogeneic lymphocytes, which were co-cultured with IL-10-treated immature dendritic cells in the first mixed leukocyte culture (MLC), were cultured with mature dendritic cells of the same origin as IL-10-treated immature dendritic cells in the second MLC. By co-culturing with IL-10-treated immature dendritic cells, the response of normal lymphocytes to mature dendritic cells cultured from the same individual as that of IL-10-treated dendritic cells was markedly reduced, compared with the lymphocytes cultured with non-treated dendritic cells or IL-10-treated dendritic cells from a third party individual. The present study demonstrated that antigen specific T cell anergy was generated by priming allogeneic lymphocytes with IL-10-treated immature dendritic cells. These data suggested the applicability of IL-10-treated recipient dendritic cells for the induction of recipient cell-specific donor T cell anergy in donor graft.
Zheng
Zhiyin
Z
First Department of Internal Medicine, Faculty of Medicine, Niigata University, 2-746, Asahimachi, Niigata, Japan.
Narita
Miwako
M
Takahashi
Masuhiro
M
Liu
Aichun
A
Furukawa
Tatsuo
T
Toba
Ken
K
Aizawa
Yoshifusa
Y
D017635:Clonal Anergy / Q000276:immunology*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D016753:Interleukin-10