title: Herpes simplex virus type 1 inoculation enhances hippocampal excitability and seizure susceptibility in mice.
abstract: Herpes simplex virus type 1 (HSV-1) is the major pathogen related to epilepsy. However, little is known about the pathogenesis of HSV-1-associated epilepsy. Here, we report that corneal inoculation of mice with HSV-1 induces acute spontaneous behavioural and electrophysiological seizures and chronically increases hippocampal excitability and seizure susceptibility. In slices from infected mice, the surviving hippocampal CA3 pyramidal neurons exhibited a more depolarizing resting membrane potential concomitant with an increase in membrane input resistance. They also had a lower threshold for generating synchronized bursts and a decrease in the amplitude of afterhyperpolarization (AHP) than did controls. These results suggest that a direct change in the excitability of the hippocampal CA3 neuronal network could play an important role in facilitating the development of acute seizures and subsequent epilepsy.
authors.affiliation: Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Hung-Ming
authors.initials: HM
authors.lastname: Wu
authors.forename: Chiung-Chun
authors.initials: CC
authors.lastname: Huang
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Ying-Ching
authors.initials: YC
authors.lastname: Liang
authors.forename: Jing-Jane
authors.initials: JJ
authors.lastname: Tsai
authors.forename: Ching-Liang
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Kuei-Sen
authors.initials: KS
authors.lastname: Hsu
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D015984:Causality; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D017097:Electric Impedance; D020803:Encephalitis, Herpes Simplex / Q000150:complications* / Q000503:physiopathology; D004827:Epilepsy / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D004833:Epilepsy, Temporal Lobe / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D007608:Kainic Acid; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009434:Neural Pathways / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000276:immunology / Q000473:pathology / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D007608:Kainic Acid