title: A C. elegans CLIC-like protein required for intracellular tube formation and maintenance.
abstract: The Caenorhabditis elegans excretory canal is composed of a single elongated and branched cell that is tunneled by an inner lumen of apical character. Loss of the exc-4 gene causes a cystic enlargement of this intracellular tube. exc-4 encodes a member of the chloride intracellular channel (CLIC) family of proteins. EXC-4 protein localizes to various tubular membranes in distinct cell types, including the lumenal membrane of the excretory tubes. A conserved 55-amino acid domain enables EXC-4 translocation from the cytosol to the lumenal membrane. The tubular architecture of this membrane requires EXC-4 for both its formation and maintenance.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Berry
authors.forename: Hannes E
authors.initials: HE
authors.lastname: Bülow
authors.forename: David H
authors.initials: DH
authors.lastname: Hall
authors.forename: Oliver
authors.initials: O
authors.lastname: Hobert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology* / Q000254:growth & development / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D018118:Chloride Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006358:Hot Temperature; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D010873:Pinocytosis; D011401:Promoter Regions, Genetic; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: C464459:CLIC4 protein, human; D029742:Caenorhabditis elegans Proteins; D018118:Chloride Channels; C480086:EXC-4 protein, C elegans; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins