Establishing insulin 1 and insulin 2 knockout congenic strains on NOD genetic background.
As insulin is a major autoantigen in autoimmune diabetes and because the insulin gene region locus in humans has been linked to diabetes risk, we have bred insulin gene knockouts onto the NOD mouse. Mice differ from humans in terms that they express two nonallelic genes of insulin. Insulin 2 is the murine homologue of the human insulin gene and is located on mouse chromosome 7. Insulin 1 is thought to have evolved by a gene duplication event, lacks the second intron of the insulin 2 gene, and is located on mouse chromosome 19. The differential thymic expression of the insulin gene may be important for central tolerance induction. Here, we present the initial establishment of congenic knockouts and characterization of the congenic intervals corresponding to insulin 1 and insulin 2 knockout genes on mouse chromosome 19 and 7, respectively.
Paronen
Johanna
J
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.
Moriyama
Hiroaki
H
Abiru
Norio
N
Sikora
Kamila
K
Melanitou
Evie
E
Babu
Sunanda
S
Bao
Fei
F
Liu
Edwin
E
Miao
Dongmei
D
Eisenbarth
George S
GS
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007328:Insulin / Q000235:genetics / Q000502:physiology*; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout
D017931:DNA Primers; D007328:Insulin