title: [Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats].
abstract: OBJECTIVE
  To explore the pathogenesis of postburn intestinal mucosal injury in scalded rats.
  
  
  METHODS
  The rats inflicted with full thickness burn were employed as the model. The two-dimensional electrophoresis (2-DE) was employed to identify the down-regulating proteins from the differential proteins in scalded rats. Spot detection and matching were performed with Image Master 2D Elite. Mass spectrometry was performed on Bruker BIFLEX III TOF.
  
  
  RESULTS
  There are 34 points of proteins in intestinal mucosa in scalded rats which were down-regulated at 6 and 12 postburn hours. Among them 22 proteins were employed for the identification and analysis. Mitochondrial aconitase, alpha-propionyl -CoA carboxylase heavy chain A of F1-ATPase in rat liver, Troponin short-chain of hydroxyacyl-coenzyme A dehydrogenase, alpha-subunit of P-electronic transferring flavoprotein (ETF) were down-regulating proteins correlated with mitochondria in intestinal mucosa in severely scalded rats. Triosephosphate isomerase 1 and cytosolic epoxide hydrolase were down-regulating proteins participated in metabolism of scalded rats. Fibromodulin, dynein-like protein, Troponin-2 and myosin light chain 3 alkali (MLC) were down-regulating proteins correlated with cellular skeletal protein. Glucocorticoid-inducible protein, nuclear factor1-B2, BRCA1, transcriptive factor EB (estradiol benzoate), beta2 subunit of G-protein, N-methyl-D-aspartate receptor1 (NMDAR) were down-regulating proteins participated in postburn regulation of intestinal mucosa in scalded rats. T-cell receptor-V-delta 6 and Ig heavy chain V region protein 1 were down-regulating proteins correlated with the immunomodulation of intestinal mucosa in scalded rats.
  
  
  CONCLUSIONS
  The down-regulating proteins of intestinal mucosa in scalded rats exhibited close relationship with mitochondria.
authors.affiliation: Department of Surgery, No.6 Hospital, Beijing 100007, P.R. China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Sun
authors.forename: Qin-Xue
authors.initials: QX
authors.lastname: Ding
authors.forename: Zhe
authors.initials: Z
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D006728:Hormones / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000378:metabolism
chemical_list: D016207:Cytokines; D006728:Hormones; D020543:Proteome