Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons. Modulation during neuronal apoptosis.
Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC1 for Rb-binding site, removing HDAC1 from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis.
Barbato
Christian
C
Istituto di Neurobiologia e Medicina Molecolare, CNR, Viale Marx 43, 00137 Rome, Italy.
Corbi
Nicoletta
N
Canu
Nadia
N
Fanciulli
Maurizio
M
Serafino
Annalucia
A
Ciotti
MariaTeresa
M
Libri
Valentina
V
Bruno
Tiziana
T
Amadoro
Giuseppina
G
De Angelis
Roberta
R
Calissano
Pietro
P
Passananti
Claudio
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002531:Cerebellum / Q000201:enzymology / Q000378:metabolism; D000071877:Checkpoint Kinase 1; D002522:Chlorocebus aethiops; D004285:Dogs; D006801:Humans; D009154:Mutation; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016160:Retinoblastoma Protein / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
D016160:Retinoblastoma Protein; D016875:tau Proteins; D011494:Protein Kinases; D000071877:Checkpoint Kinase 1