Phenotype of peroxisome proliferator-activated receptor-alpha(PPARalpha)deficient mice on mixed background fed high fat diet.
Considerable controversy exists in determining the role of peroxisome proliferator-activated receptor-alpha PPARalpha) on obesity. Previous reports demonstrated that PPARalpha is a critical modulator of lipid homeostasis, but the overt, obese phenotypic characterization in the strain of PPAR deficient (PPARalpha-/-) mice is influenced by other factors, including diet and genetics. Therefore, it is necessary to establish the phenotypic characterization of PPARalpha-/- mice prior to the obesity-related study. In this study, we observed phenotype of PPARalpha-/- mice on mixed genetic background (C57BL/6Nx129/Sv) fed a high fat diet for 16 weeks. PPARalpha-/- mice, regardless of sex, raised body growth rate significantly comparing with wild type and showed male-specific fatty change in the liver. They were shown to lack hepatic induction of PPARalpha target genes encoding enzymes for fatty acid beta-oxidation.
Kim
Bang Hyun
BH
Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Korea.
Won
Young Suk
YS
Kim
Eun-Young
EY
Yoon
Mijung
M
Nam
Ki-Taek
KT
Oh
Goo Taeg
GT
Kim
Dae-Yong
DY
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D003433:Crosses, Genetic; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D012313:RNA / Q000737:chemistry / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D014280:Triglycerides; D012313:RNA; D002784:Cholesterol