Imaging of (13)C-labeled glucose and sorbitol in bovine lens by (1)H-detected (13)C nuclear magnetic resonance spectroscopy.
Bovine lenses were incubated in a solution containing [1-(13)C]glucose (50 mM) for 1, 2 and 4 days. Spectroscopic images of [1-(13)C]glucose and [1-(13)C]sorbitol were constructed using (1)H-detected gradient-enhanced heteronuclear multiple-quantum coherence (GE-HMQC) in a 2.0-tesla magnetic field. Accumulations of [1-(13)C]glucose and [1-(13)C]sorbitol were mainly observed at the periphery of the lens. Their distributions corresponded to the cortex. (1)H-detected (13)C nuclear magnetic resonance (NMR) spectroscopic imaging by GE-HMQC successfully demonstrated the distribution of [1-(13)C]glucose and [1-(13)C]sorbitol at the periphery of bovine lenses.
Sawada
Tomoko
T
Department of Ophthalmology, Shiga University of Medical Science, Shiga, Japan. tsawada@belle.shiga-med.ac.jp
Nakamura
Jiro
J
Nishida
Yasuhiro
Y
Kani
Kazutaka
K
Okamoto
Ryohei
R
Morikawa
Shigehiro
S
Inubushi
Toshiro
T
D000818:Animals; D002417:Cattle; D005947:Glucose / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D023421:Models, Animal; D013012:Sorbitol / Q000378:metabolism*
D013012:Sorbitol; D005947:Glucose