A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.
Synapse-specific facilitation requires rapamycin-dependent local protein synthesis at the activated synapse. In Aplysia, rapamycin-dependent local protein synthesis serves two functions: (1) it provides a component of the mark at the activated synapse and thereby confers synapse specificity and (2) it stabilizes the synaptic growth associated with long-term facilitation. Here we report that a neuron-specific isoform of cytoplasmic polyadenylation element binding protein (CPEB) regulates this synaptic protein synthesis in an activity-dependent manner. Aplysia CPEB protein is upregulated locally at activated synapses, and it is needed not for the initiation but for the stable maintenance of long-term facilitation. We suggest that Aplysia CPEB is one of the stabilizing components of the synaptic mark.
Si
Kausik
K
Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York State Psychiatric Institute, 722 West 168th Street, New York, NY 10032, USA. ks560@columbia.edu
Giustetto
Maurizio
M
Etkin
Amit
A
Hsu
Ruby
R
Janisiewicz
Agnieszka M
AM
Miniaci
Maria Conchetta
MC
Kim
Joung-Hun
JH
Zhu
Huixiang
H
Kandel
Eric R
ER
D000199:Actins / Q000235:genetics; D000818:Animals; D001048:Aplysia; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017774:Long-Term Potentiation / Q000502:physiology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology; D020033:Protein Isoforms / Q000378:metabolism; D020871:RNA Stability / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D029867:Xenopus Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors
D000199:Actins; C490388:Cpeb1 protein, Xenopus; D018076:DNA, Complementary; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors; D029867:Xenopus Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D011061:Poly A; D012701:Serotonin; D017868:Cyclic AMP-Dependent Protein Kinases; D022021:Tacrolimus Binding Proteins