[A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province].
OBJECTIVE
To identify subtypes of human immunodeficiency virus 1 (HIV-1) strains and their distribution, infection sources, and the trends of HIV infection in Jiangsu province.


METHODS
Anticongulated bloods from 46 infected persons were collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes. The analysis of consensus sequence, genetic distance and phylogenetic tree were conducted with GCG software.


RESULTS
By the end of 2001, there had been six subtypes of HIV-1 strains identified in Jiangsu province: A, B, B', C, D and E. The predominant subtypes were C (accounting for 40.48%) and B' (accounting for 38.10%). Subtype C accounted for 86.67% among injecting DUs while subtype B' accounted for 91.67% among commercial blood donors and receivers.


CONCLUSIONS
Subtype B'among commercial blood donors was brought to Jiangsu from neighboring provinces. The outbreak of HIV-1 infection among local DUs was caused by subtype C from Xinjiang province. Findings from HIV/AIDS molecular epidemiologic study suggest that it is challenging for Jiangsu to treat patients, apply vaccine, prevent and control AIDS in the future.
Yang
Hai-tao
HT
Jiangsu Province Center for Diseases Prevention and Control, Nanjing 210009, China.
Xing
Hui
H
Jia
Cheng-mei
CM
Liang
Hao
H
Xu
Xiao-qin
XQ
Wei
Ming
M
Li
Lei
L
Chen
Zhao
Z
Shao
Yi-ming
YM
D002681:China / Q000453:epidemiology; D015752:Genes, env / Q000235:genetics; D015658:HIV Infections / Q000097:blood* / Q000453:epidemiology; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA