The mouse Murr1 gene is imprinted in the adult brain, presumably due to transcriptional interference by the antisense-oriented U2af1-rs1 gene.
The mouse Murr1 gene contains an imprinted gene, U2af1-rs1, in its first intron. U2af1-rs1 shows paternal allele-specific expression and is transcribed in the direction opposite to that of the Murr1 gene. In contrast to a previous report of biallelic expression of Murr1 in neonatal mice, we have found that the maternal allele is expressed predominantly in the adult brain and also preferentially in other adult tissues. This maternal-predominant expression is not observed in embryonic and neonatal brains. In situ hybridization experiments that used the adult brain indicated that Murr1 gene was maternally expressed in neuronal cells in all regions of the brain. We analyzed the developmental change in the expression levels of both Murr1 and U2af1-rs1 in the brain and liver, and we propose that the maternal-predominant expression of Murr1 results from transcriptional interference of the gene by U2af1-rs1 through the Murr1 promoter region.
Wang
Youdong
Y
Department of Biomolecular Sciences, Saga Medical School, Saga 849-8501, Japan.
Joh
Keiichiro
K
Masuko
Sadahiko
S
Yatsuki
Hitomi
H
Soejima
Hidenobu
H
Nabetani
Akira
A
Beechey
Colin V
CV
Okinami
Satoshi
S
Mukai
Tsunehiro
T
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001921:Brain / Q000378:metabolism; D018392:Genomic Imprinting; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF
D048868:Adaptor Proteins, Signal Transducing; C103799:Commd1 protein, mouse; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse