title: Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles.
abstract: Fe3O4 nanoparticles cast on pyrolytic graphite (PG) electrodes were used to immobilize hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP). The Fe3O4 nanoparticles provided a favorable microenvironment for the proteins to directly transfer electrons with electrodes. The protein-Fe3O4 films were used to electrochemically catalyze the reduction of oxygen, trichloroacetic acid, nitrite and hydrogen peroxide, and showed a potential applicability in fabricating biosensors. Transmission electron microscopy (TEM), UV-visible absorption and reflectance absorption infrared (RAIR) spectroscopy, and cyclic and square wave voltammetry, were used to characterize the films.
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Cao
authors.forename: Pingli
authors.initials: P
authors.lastname: He
authors.forename: Naifei
authors.initials: N
authors.lastname: Hu
mesh_terms: D015374:Biosensing Techniques; D001709:Biotechnology; D004563:Electrochemistry / Q000379:methods; D005290:Ferric Compounds; D006420:Hemeproteins; D008832:Microchemistry / Q000379:methods
chemical_list: D005290:Ferric Compounds; D006420:Hemeproteins; C000499:ferric oxide