[Application of polymorphism of HLA-DR locus in personal identification].
The aim of this paper was to present the results of investigation of the high polymorphic HLA-DR locus by the PCR-SSP method. Genomic DNA was isolated from whole blood, tissue samples (muscles and brains). HLA-DR alleles were typed using the DYNAL DR "low resolution" SSP kit. PCR products were separated on agarose gel with ethidium bromide and estimated in ultraviolet light. Phenotypic and genotypical rates of blood and tissues have been analysed. After electrophoresis in 228 DNA samples 39 phenotypes and 10 alleles were identified. The most frequent phenotypes of the HLA-DR locus were 5/6, 6/6 and 3/5. The high frequency of HLA-DR 6, DR 5 and DR 7 alleles have been found. No deviations from the Hardy-Weinberg equilibrium were observed. The HLA-DR locus has a relatively high information content (PIC), power of discrimination (PD) and mean exclusion chance (MEC). The molecular PCR-SSP method allows for very rapid and highly discriminatory HLA analysis. We found the PCR-SSP technique very useful in routine HLA-DR identification.
Wysocka
Joanna
J
Katedry i Zakładu Medycyny Sadowej AM w Gdańsku.
Szczerkowska
Zofia
Z
D000483:Alleles; D016172:DNA Fingerprinting / Q000379:methods*; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013997:Time Factors
D006684:HLA-DR Antigens