Mid-infrared spectroscopic measurement of ionic dissociative materials in the metabolic pathway.
We determine the pH dependency of the mid-infrared spectra in aqueous solution of the organic dissociative materials in the metabolic pathway: saccharide phosphates (G6P, F6P), adenosine, and its phosphates (ATP, ADP, AMP). The series of molar absorbance spectra for these reagents were obtained in a pH range of about 2 to 11 with a Fourier transform infrared (FT-IR) spectrometer equipped with a horizontal diamond attenuated total reflection (ATR) sampling accessory. We also provide a method of infrared spectral extraction of ionic dissociative materials by performing a linear least-square fitting utilizing the formulas of ionic dissociation equilibrium shift, and we obtain the infrared spectrum of each ionic species of the dissociative materials: G6P-, G6P2-; F6P-, F6P2-; ATP2-, ATP3-, ATP4-; ADP-, ADP2-, ADP3-; AMP, AMP-, AMP2-; and adenosine+, adenosine0. The infrared spectral structure of each ionic species of the dissociative materials in the metabolic pathway are discussed. Additionally, the possibility for a quantification system of the concentrations of the organic dissociative materials in varying pH is suggested.
Nakanishi
Kenichi
K
Department of Sustainable Resource Science, Faculty of Bioresources, Mie University, 1515 Kamihama-cho, Tsu, Mie, 514-8507 Japan.
Hashimoto
Atsushi
A
Pan
Tao
T
Kanou
Mikihito
M
Kameoka
Takaharu
T
D000244:Adenosine Diphosphate / Q000032:analysis / Q000737:chemistry; D000249:Adenosine Monophosphate / Q000032:analysis / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000032:analysis / Q000737:chemistry; D005636:Fructosephosphates / Q000032:analysis / Q000737:chemistry; D019298:Glucose-6-Phosphate / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008660:Metabolism; D015203:Reproducibility of Results; D012996:Solutions / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*
D005636:Fructosephosphates; D007477:Ions; D012996:Solutions; D000249:Adenosine Monophosphate; D019298:Glucose-6-Phosphate; D000244:Adenosine Diphosphate; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate