Islet cell engraftment and control of diabetes in rats after transplantation of pig pancreatic anlagen.
The insufficient supply of tissue, loss posttransplantation, and limited potential for expansion of beta-cells restrict the use of islet allotransplantation for diabetes. A way to overcome the supply and expansion problems is to xenotransplant embryonic tissue. We have shown that whole rat pancreatic anlagen isotransplanted into the omentum of rats, or xenotransplanted into costimulatory blocked mice, undergo growth and differentiate into islets surrounded by stoma without exocrine tissue. Isotransplants normalize glucose tolerance in diabetic hosts. Here, we show that embryonic day 29 porcine pancreas transplanted into the omentum of adult diabetic rats undergoes endocrine tissue differentiation over 20 wk and normalizes body weights and glucose tolerance. Unlike rat-to-rodent transplants, individual alpha- and beta-cells engraft without a stromal component, and no immunosuppression is required for pig-to-rat transplants. Herein is described a novel means to effect the xenotransplantation of individual islet cells across a highly disparate barrier.
Rogers
Sharon A
SA
Renal Division, Department of Medicine, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
Chen
Feng
F
Talcott
Mike
M
Hammerman
Marc R
MR
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D005260:Female; D005951:Glucose Tolerance Test; D007165:Immunosuppression Therapy; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000196:embryology* / Q000502:physiology*; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000502:physiology*; D009852:Omentum / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014183:Transplantation, Heterologous; D015430:Weight Gain / Q000502:physiology
D007328:Insulin; D012333:RNA, Messenger