title: Steroids and adrenomedullin growth patterns in human ovarian cancer cells: estrogenic-regulation assay.
abstract: OBJECTIVE
  Adrenomedullin is a multifunctional regulatory peptide with mitogenic and angiogenic capabilities. Objectives in this study were: (1) to describe the effects of adrenomedullin and anti-adrenomedullin antibodies on ovarian carcinoma cell proliferation, and (2) to assess the estradiol regulation of adrenomedullin metabolism.
  
  
  METHODS
  We assessed the effects of estradiol, adrenomedullin, and anti-adrenomedullin antibodies on cell growth in three human ovarian cell lines. RT-PCR was used to assess mRNA expression and Western blots to determine protein levels.
  
  
  RESULTS
  Estradiol stimulates BG-1 and PEO4 cells growth but not PEO14 cells. Adrenomedullin mRNA expression and secretion were not under estrogen control. Adrenomedullin and anti-adrenomedullin antibodies had no growth effects in vitro. Adjunction of anti-adrenomedullin antibodies to estradiol-stimulated cells significantly inhibited their growth.
  
  
  CONCLUSIONS
  Adrenomedullin metabolism is not under estradiol control. Anti-adrenomedullin antibodies display inhibitory effects on cells having high mitogenic activity. This opens the need for additional search toward in vivo specific immunotherapy.
authors.affiliation: Department of Obstetrics and Gynecology (Pr F Laffargue), Hôpital Arnaud de Villeneuve, University of Montpellier, Montpellier, France. pl.giacalone@wanadoo.fr
authors.forename: Pierre-Ludovic
authors.initials: PL
authors.lastname: Giacalone
authors.forename: J P
authors.initials: JP
authors.lastname: Daurés
authors.forename: L'Houcine
authors.initials: L
authors.lastname: Ouafik
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Martin
authors.forename: François
authors.initials: F
authors.lastname: Laffargue
authors.forename: Thierry
authors.initials: T
authors.lastname: Maudelonde
mesh_terms: D053607:Adrenomedullin; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D004958:Estradiol / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010455:Peptides / Q000235:genetics / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000906:Antibodies; D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin; D004958:Estradiol