title: Evaluation of cell culture flasks designed for experiment under altered gravity-vector conditions.
abstract: Cell culture flasks applicable for altered gravity conditions, such as centrifugation, clino-rotation or microgravity in space, were manufactured for trial. The flask has flat polystyrene surface for monolayer culture and gas-permeable film window on the opposite face. The space in-between consists the culture chamber to be filled with liquid medium. To reduce the water loss and bubble formation in the culture fluid, another gas permeable window was placed on top to form a space where distilled water may be filled. The double-decker culture flask can be used for both space and ground-based experiments in common.
authors.affiliation: ISS Science Project Office, ISAS, JAXA.
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Gyotoku
authors.forename: Mutsumu
authors.initials: M
authors.lastname: Nagase
authors.forename: Noboru
authors.initials: N
authors.lastname: Ando
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tanigaki
authors.forename: Muneo
authors.initials: M
authors.lastname: Takaoki
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D019411:Clinical Laboratory Techniques / Q000295:instrumentation*; D004867:Equipment Design; D018470:Gravity, Altered; D012106:Research / Q000295:instrumentation*; D013026:Space Flight / Q000295:instrumentation; D014893:Weightlessness