title: Correlations between pulp properties of eucalyptus clones and leaf volatiles using automated solid-phase microextraction.
abstract: Analysis of biogenic volatile organic compounds (BVOC) of 14 Eucalyptus clones has been performed using an automated headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography (GC)/ion trap mass spectrometry (ITMS) method. Correlations between pulp properties of Eucalyptus clones and the BVOC of their leaf headspaces were studied. The compounds alpha-terpineol and the sesquiterpene beta-eudesmol were positively correlated with S5, a property related to the hemicelluose content in the pulp. Qualitative results obtained with automated HS-SPME were sufficient to group together the same species and related hybrids through cluster analysis and were confirmed through principal component analysis. A preliminary separation of the essential oils of Eucalyptus dunnii through comprehensive two-dimensional gas chromatography (GC x GC) showed approximately 580 peaks compared to approximately 60 in a typical GC/ITMS first-dimension chromatogram. The potential of HS-SPME coupled to GC x GC to improve the separation of Eucalyptus volatiles and other plant essential oils looks extremely promising for new applications of unsupervised learning methods.
authors.affiliation: Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Cláudia A
authors.initials: CA
authors.lastname: Zini
authors.forename: Teotônio F
authors.initials: TF
authors.lastname: De Assis
authors.forename: Edward B
authors.initials: EB
authors.lastname: Ledford
authors.forename: Claudio
authors.initials: C
authors.lastname: Dariva
authors.forename: Jandyra
authors.initials: J
authors.lastname: Fachel
authors.forename: Eva
authors.initials: E
authors.lastname: Christensen
authors.forename: Janusz
authors.initials: J
authors.lastname: Pawliszyn
mesh_terms: D001322:Autoanalysis; D001947:Breeding; D002849:Chromatography, Gas; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D010944:Plants / Q000737:chemistry* / Q000235:genetics*; D012641:Selection, Genetic; D045787:Sesquiterpenes, Eudesmane / Q000032:analysis; D013729:Terpenes / Q000032:analysis
chemical_list: D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D045787:Sesquiterpenes, Eudesmane; D013729:Terpenes; C016775:alpha-terpineol; C051082:beta-eudesmol