A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.
Deletions of three yeast genes, SET2, CDC73, and DST1, involved in transcriptional elongation and/or chromatin metabolism were used in conjunction with genetic array technology to screen approximately 4700 yeast deletions and identify double deletion mutants that produce synthetic growth defects. Of the five deletions interacting genetically with all three starting mutations, one encoded the histone H2A variant Htz1 and three encoded components of a novel 13 protein complex, SWR-C, containing the Snf2 family ATPase, Swr1. The SWR-C also copurified with Htz1 and Bdf1, a TFIID-interacting protein that recognizes acetylated histone tails. Deletions of the genes encoding Htz1 and seven nonessential SWR-C components caused a similar spectrum of synthetic growth defects when combined with deletions of 384 genes involved in transcription, suggesting that Htz1 and SWR-C belong to the same pathway. We show that recruitment of Htz1 to chromatin requires the SWR-C. Moreover, like Htz1 and Bdf1, the SWR-C promotes gene expression near silent heterochromatin.
Krogan
Nevan J
NJ
Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6.
Keogh
Michael-Christopher
MC
Datta
Nira
N
Sawa
Chika
C
Ryan
Owen W
OW
Ding
Huiming
H
Haw
Robin A
RA
Pootoolal
Jeffrey
J
Tong
Amy
A
Canadien
Veronica
V
Richards
Dawn P
DP
Wu
Xiaorong
X
Emili
Andrew
A
Hughes
Timothy R
TR
Buratowski
Stephen
S
Greenblatt
Jack F
JF
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D015825:Chromosomes, Fungal; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D035165:Transcription Factors, General / Q000235:genetics / Q000378:metabolism; D035602:Transcriptional Elongation Factors
C104439:CDC73 protein, S cerevisiae; D004268:DNA-Binding Proteins; D006657:Histones; D046911:Macromolecular Substances; D009687:Nuclear Proteins; C499336:SMARCA1 protein, human; C084011:SMARCA2 protein, human; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D008780:Methyltransferases; C448996:Set2 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C084108:SMARCA4 protein, human; C499333:SNF2 protein, S cerevisiae; D004265:DNA Helicases