[Establishment of a mouse model of cyclophosphamide-induced thrombocytopenia and determination of platelet function in this model].
OBJECTIVE
To establish a mouse model of acute hematopoietic failure and explore the pathological basis of platelet changes in bone marrow suppression.


METHODS
An initial large dose of cyclophosphamide (200 mg/kg) was injected through the tail veins of the mice, follow by daily intraperitoneal injection starting on the next day for 7 d. The number and morphology of the cells in the peripheral blood and bone marrow were observed by means of cell counting and smear, respectively, with the platelet aggregation determined using ADP. The coagulation time was measure by turbidimetry.


RESULTS
The amount of platelets, erythrocytes, leukocytes and the nucleated cells in mice bone marrow was significantly lowered in the mouse models in comparison with the control mice, and the normal hematopoietic tissues were depressed. The blood platelets in the models were lowered by 49%, an amplitude of declination significantly greater than that occurring in the erythrocytes and leukocytes (by 28% and 25% respectively). Though the mean platelet volume and percent platelet aggregation underwent no obvious changes, the coagulation time was significantly shortened.


CONCLUSIONS
The methods we described for establishing mouse models of hematopoietic failure induced by cyclophosphamide is rapid and highly efficient, and may facilitate ready preparation of mouse models of thrombocytopenia which respond strongly to cyclophosphamide without blood platelets function impairment.
Zhang
Qing
Q
Key Laboratory of Genetic Engineering of Education Ministry, Sun Yat-sun University, Guangzhou 510275, China. ls80@zsu.edu.cn
Peng
Hong-juan
HJ
Yu
Xiao-bing
XB
Xu
Pei-ling
PL
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D001856:Bone Marrow Examination; D003520:Cyclophosphamide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013921:Thrombocytopenia / Q000097:blood / Q000139:chemically induced*
D003520:Cyclophosphamide