title: MIC-A genotypes 4/5.1 and 9/9 are positively associated with type 1 diabetes mellitus in Brazilian population.
abstract: The aim of our study was to evaluate the frequencies of MIC-A alleles and genotypes in Brazilian patients with type 1 diabetes mellitus (T1DM) and healthy controls. MHC class I chain-related gene-A (MIC-A) has been shown to be associated with susceptibility to T1DM in different populations. We analyzed the DNA samples from 86 patients and 201 healthy controls for MIC-A by PCR amplification, and fragment sizes were determined in an ABI prism DNA sequencer. We found increased frequencies of two genotypes, MIC-A 4/5.1 (pc = 0.006; OR, 6.1) and 9/9 (pc = 0.045; OR, 5.75), in our patients (mean diagnosis age, 11.70 years; SD, 8.86; mean age, 20.44 years; SD, 12.17) compared with the controls (median age, 26.41 years; SD, 8.87).
authors.affiliation: Department of Biochemistry, Faculty of Biology, University of Bucharest, Bucharest, Romania.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tica
authors.forename: Liene
authors.initials: L
authors.lastname: Nikitina-Zake
authors.forename: Eduardo
authors.initials: E
authors.lastname: Donadi
authors.forename: Carani B
authors.initials: CB
authors.lastname: Sanjeevi
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005805:Genes, MHC Class I; D005838:Genotype; D006801:Humans
chemical_list: D017931:DNA Primers