Neuronal calcium sensor-1 is expressed by dorsal root ganglion cells, is axonally transported to central and peripheral terminals, and is concentrated at nodes.
Neuronal calcium sensor-1 (NCS-1) is a member of the EF-hand calcium-binding protein superfamily which has been implicated in the modulation of a number of neuronal functions. In this study we have examined the expression of NCS-1 in adult rat dorsal root ganglion (DRG) neurons. NCS-1 immunoreactivity was present in most DRG neurons, including many calcitonin gene-related peptide (CGRP) expressing ones. NCS-1 showed some colocalization with the synaptic vesicle protein synaptophysin and underwent both anterograde and retrograde axonal transport. NCS-1 immunoreactivity was also present in the dorsal horn of the spinal cord, and in peripheral cutaneous terminals innervating blood vessels, where it was coexpressed with CGRP. In addition, NCS-1 in peripheral nerves was concentrated at nodes and adjoining paranodes. These results suggest novel roles for NCS-1, particularly in relation to channel function at nodes and to the peripheral release of vasoactive peptides.
Averill
S
S
Neuroscience Centre, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK.
Robson
L G
LG
Jeromin
A
A
Priestley
J V
JV
D000818:Animals; D001370:Axonal Transport; D001808:Blood Vessels / Q000294:innervation; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051580:Neuronal Calcium-Sensor Proteins; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism; D011901:Ranvier's Nodes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000378:metabolism; D012867:Skin / Q000294:innervation; D013116:Spinal Cord / Q000378:metabolism; D016708:Synaptophysin
D002135:Calcium-Binding Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D016708:Synaptophysin; C101464:frequenin calcium sensor proteins; D015740:Calcitonin Gene-Related Peptide