title: Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line.
abstract: This study was designed to determine the antiproliferative, apoptotic and antioxidative properties of crude methanolic extract (CME) from the pericarp of Garcinia mangostana (family Guttiferae) using human breast cancer (SKBR3) cell line as a model system. SKBR3 cells were cultured in the presence of CME at various concentrations (0-50 microg/ml) for 48 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyl tetrazolium bromide (MTT) assay. CME showed a dose-dependent inhibition of cell proliferation with ED(50) of 9.25+/-0.64 microg/ml. We found that antiproliferative effect of CME was associated with apoptosis on breast cancer cell line by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, CME at various concentrations and incubation times were also found to inhibit ROS production. These investigations suggested that the methanolic extract from the pericarp of Garcinia mangostana had strong antiproliferation, potent antioxidation and induction of apoptosis. Thus, it indicates that this substance can show different activities and has potential for cancer chemoprevention which were dose dependent as well as exposure time dependent.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Mahidol University, Sri Ayudthaya Road, Rajdhevee, Bangkok 10400, Thailand. pypmk@mahidol.ac.th
authors.forename: Primchanien
authors.initials: P
authors.lastname: Moongkarndi
authors.forename: Nuttavut
authors.initials: N
authors.lastname: Kosem
authors.forename: Sineenart
authors.initials: S
authors.lastname: Kaslungka
authors.forename: Omboon
authors.initials: O
authors.lastname: Luanratana
authors.forename: Narongchai
authors.initials: N
authors.lastname: Pongpan
authors.forename: Neelobol
authors.initials: N
authors.lastname: Neungton
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D029760:Garcinia; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species