kappa-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase.
kappa-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K(+). Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer-dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.
Mondal
Kalyani
K
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Roy
Ipsita
I
Gupta
Munishwar N
MN
D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification*; D002351:Carrageenan / Q000737:chemistry*; D011232:Chemical Precipitation; D004396:Coloring Agents / Q000737:chemistry; D008024:Ligands; D011188:Potassium / Q000494:pharmacology; D012995:Solubility / Q000187:drug effects
D004396:Coloring Agents; D008024:Ligands; D002351:Carrageenan; D000426:Alcohol Dehydrogenase; D011188:Potassium