[Some aspects of determination of the sensitivity of Mycobacterium tuberculosis to ethambutol using a semiliquid medium].
In addition to the earlier developed rapid technique for determining the drug resistance of Mycobacterium tuberculosis (MBT) to essential antituberculous drugs by using semiliquid medium (SLM), the author proposes a procedure for determining their resistance to ethambutol. She shows it necessary to use the drug only as a powder. The mean minimum suppressing concentrations of ethambutol in the semiliquid medium have been determined for stock cultures, sensitive and resistant strains, which are 3.5 +/- 2.5, 4.5 +/- 1.4, 8.5 +/- 1.6 micrograms/ml. Parallel determination of the sensitivity of 91 MBT strains by using the standard Löwenstein-Jensen medium and SLM has yielded the optimum concentration of ethambutol (5 micrograms/ml), which is marked by the highest (84.4%) percent of coincidence of results.
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D000995:Antitubercular Agents / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004977:Ethambutol / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011208:Powders; D013607:Tablets; D013997:Time Factors
D000995:Antitubercular Agents; D003470:Culture Media; D011208:Powders; D013607:Tablets; D004977:Ethambutol