title: Cortical spreading depression blocks the hyperthermic reaction induced by orexin A.
abstract: This experiment tested the effect of cortical spreading depression on the sympathetic and thermogenic effects induced by orexin A. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before and 5 h after an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle. The same variables were monitored in rats with cortical spreading depression, induced by an application of cotton pellets soaked with 2 M KCl to the frontal cortex. The results show that orexin A increases the sympathetic firing rate, IBAT and colonic temperatures and heart rate. The increases in firing rate, IBAT and colonic temperatures are blocked by cortical spreading depression, while the increase in heart rate is not affected by cortical spreading depression. These findings suggest that the cerebral cortex is involved in the control of the orexin A-induced hyperthermia. Furthermore, we suggested the name "hyperthermine A," as additional denomination of "orexin A."
authors.affiliation: Department of Experimental Medicine, Section of Human Physiology, Second University of Naples, via Costantinopoli 16, 80138 Naples, Italy. marcellino.monda@unina2.it
authors.forename: M
authors.initials: M
authors.lastname: Monda
authors.forename: A N
authors.initials: AN
authors.lastname: Viggiano
authors.forename: A L
authors.initials: AL
authors.lastname: Viggiano
authors.forename: F
authors.initials: F
authors.lastname: Fuccio
authors.forename: V
authors.initials: V
authors.lastname: De Luca
mesh_terms: D002001:Adipose Tissue, Brown / Q000294:innervation; D000818:Animals; D002352:Carrier Proteins / Q000008:administration & dosage*; D002541:Cerebral Decortication; D013181:Cortical Spreading Depression; D006339:Heart Rate / Q000187:drug effects; D006979:Hyperthermia, Induced; D007276:Injections, Intraventricular; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D019579:Neocortex / Q000187:drug effects* / Q000502:physiology; D009479:Neuropeptides / Q000008:administration & dosage*; D000068797:Orexins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D022722:Thermogenesis / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; C000596229:HCRT protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins