Application of solvent engineering to optimize lipase-catalyzed 1,3-diglyacylcerols by mixture response surface methodology.
1,3-Diacylglycerol (DAG) has been introduced in Japan as a cooking oil under the trade name of Econa to reduce body fat accumulation. Solvent engineering was applied to determine the optimum solvent mixtures for the lipase-catalyzed synthesis of 1,3-DAG by mixture response surface methodology. n-Hexane was required to maintain the lipase activity and the product selectivity could be adjusted by changing the hydrophobicity of reaction medium. The optimum yield (approximately 40%) of 1,3-DAG synthesis was obtained with n-hexane/octane (1:1, v/v).
Liao
Hui-Fen
HF
Department of Medical Research, Mackay Memorial Hospital, 45 Minsheng Road, Tamshui, Taipei County, 251, Taiwan.
Tsai
Wei-Chuan
WC
Chang
Shu-Wei
SW
Shieh
Chwen-Jen
CJ
D002384:Catalysis; D002616:Chemical Engineering / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D004075:Diglycerides / Q000138:chemical synthesis* / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D011786:Quality Control; D012997:Solvents / Q000737:chemistry*; D014304:Triolein / Q000737:chemistry*
D004075:Diglycerides; D006586:Hexanes; D012997:Solvents; D014304:Triolein; C026385:n-hexane; D008049:Lipase