title: 3D structure of the influenza virus polymerase complex: localization of subunit domains.
abstract: The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP-mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.
authors.affiliation: Centro Nacional de Biotecnologìa, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Area
authors.forename: Jaime
authors.initials: J
authors.lastname: Martín-Benito
authors.forename: Pablo
authors.initials: P
authors.lastname: Gastaminza
authors.forename: Eva
authors.initials: E
authors.lastname: Torreira
authors.forename: José M
authors.initials: JM
authors.lastname: Valpuesta
authors.forename: José L
authors.initials: JL
authors.lastname: Carrascosa
authors.forename: Juan
authors.initials: J
authors.lastname: Ortín
mesh_terms: D007091:Image Processing, Computer-Assisted; D009980:Influenza A virus / Q000201:enzymology* / Q000235:genetics; D008854:Microscopy, Electron; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
chemical_list: C061290:PA protein, influenza viruses; C057027:PB2 protein, Influenzavirus A; D021122:Protein Subunits; D012261:Ribonucleoproteins; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase