title: Distribution and expression of non-muscle myosin light chain kinase in rabbit livers.
abstract: OBJECTIVE
  To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.
  
  
  METHODS
  Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.
  
  
  RESULTS
  The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.
  
  
  CONCLUSIONS
  nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China.
authors.forename: Hua-Qing
authors.initials: HQ
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruo-Lei
authors.initials: RL
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Ren
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Jiang
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Gui
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011817:Rabbits
chemical_list: D009219:Myosin-Light-Chain Kinase; C482520:non-muscle myosin light chain kinase, rabbit