title: Inhibition of acetylcholinesterase activity in the terrestrial isopod Porcellio scaber as a biomarker of organophosphorus compounds in food.
abstract: This paper describes the toxicity of organophosphorus pesticide diazinon in juvenile and adult terrestrial isopods Porcellio scaber (Isopoda, Crustacea). The woodlice were exposed to different concentrations of diazinon added to food (5, 10, 50, and 100 or 150 micrograms/g dry food). Weight change and food assimilation efficiency were determined two and four weeks after the exposure. The activity of acetylcholinesterase (AChE) in surviving animals was measured at the end of the experiment. The results show that woodlice exposed to diazinon do not significantly differ from controls in growth and feeding rate. The reduction of AChE activity was observed at the lowest diazinon exposure (5 and 10 micrograms/g dry food). These results suggest that AChE activity might prove a useful biomarker, indicating low levels of organophosphates in food.
authors.affiliation: Nova Gorica Polytechnic, Nova Gorica, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Stanek
authors.forename: Elizabeta
authors.initials: E
authors.lastname: Gabrijelcic
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
authors.forename: Polonca
authors.initials: P
authors.lastname: Trebse
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D003976:Diazinon / Q000633:toxicity*; D005506:Food Contamination; D007306:Insecticides / Q000633:toxicity*; D033321:Isopoda / Q000187:drug effects / Q000201:enzymology*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D000110:Acetylcholinesterase; D003976:Diazinon