Glucose 6-phosphate dehydrogenase is required for sucrose and trehalose to be efficient osmoprotectants in Sinorhizobium meliloti.
Inactivation of the zwf gene in Sinorhizobium meliloti induces an osmosensitive phenotype and the loss of osmoprotection by trehalose and sucrose, but not by ectoine and glycine betaine. This phenotype is not linked to a defect in the biosynthesis of endogenous solutes. zwf expression is induced by high osmolarity, sucrose and trehalose, but is repressed by betaine. A zwf mutant is more sensitive than its parental strain to superoxide ions, suggesting that glucose 6-phosphate dehydrogenase involvement in the osmotic response most likely results from the production of reactive oxygen species during osmotic stress.
Barra
Lise
L
Osmorégulation chez les bactéries, CNRS UMR 6026, Université de Rennes I, Campus de Beaulieu, Av. du Général Leclerc, 35042 Rennes, France.
Pica
Nathalie
N
Gouffi
Kamila
K
Walker
Graham C
GC
Blanco
Carlos
C
Trautwetter
Annie
A
D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006982:Hypertonic Solutions; D000455:Medicago sativa; D016296:Mutagenesis; D009586:Nitrogen Fixation; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013395:Sucrose / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*; D024483:Vitamin K 3 / Q000494:pharmacology
D006982:Hypertonic Solutions; D013481:Superoxides; D013395:Sucrose; D024483:Vitamin K 3; D014199:Trehalose; D005954:Glucosephosphate Dehydrogenase