[Influence of physiologic 17 beta-estradiol concentrations on gene E6 expression in HVP type 18 in vitro].
Many recent studies indicate that long term use of contraceptives is a strong risk factor in the development of cervical cancer. Steroid hormones, in persistent papilloma virus infection act on various levels, one of them is enhancing transforming activity of the virus. The aim of the study was to estimate if physiological concentrations of 17 beta-estradiol could influence expression of viral transforming genes. HeLa cell lines were incubated with three different physiological concentrations and and on the third day of incubation the level of E6 gene expression was determined. Results show no differences in expression between the control culter, and cultures incubated with physiological concentrations. It indicates that normal levels of 17 beta-estradiol don't play role in transforming process but it also shows need to analyse higher levels of hormones by quantitative analyses in prospective studies.
Dziubińska-Parol
Izabella
I
Zakładu Genetyki Medycznej AM w Lublinie.
Gasowska
Urszula
U
Rzymowska
Jolanta
J
Kwaśniewska
Anna
A
D003278:Contraceptives, Oral, Hormonal / Q000009:adverse effects; D003588:Cytopathogenic Effect, Viral; D004268:DNA-Binding Proteins; D004958:Estradiol / Q000009:adverse effects* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000378:metabolism*; D027383:Papillomaviridae / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors; D014412:Tumor Virus Infections / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced* / Q000821:virology
D003278:Contraceptives, Oral, Hormonal; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D004958:Estradiol