Influence of growth temperature on lipid and phosphate contents of surface polysaccharides from the antarctic bacterium Pseudoalteromonas haloplanktis TAC 125.
The chemical structural variations induced by different growth temperatures in the lipooligosaccharide and exopolysaccharide components extracted from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125 are described. The increase in phosphorylation with the increase in growth temperature seems to be general, because it happens not only for the lipooligosaccharide but also for the exopolysaccharide. Structural variations in the lipid components of lipid A also occur. In addition, free lipid A is found at both 25 and 4 degrees C but not at 15 degrees C, which is the optimal growth temperature, suggesting a incomplete biosynthesis of the lipooligosaccharide component under the first two temperature conditions.
Corsaro
M Michela
MM
Dipartimento di Chimica Organica e Biochimica, Università Federico II di Napoli, Complesso Universitario Monte S. Angelo, 80126 Naples, Italy. corsaro@unina.it
Lanzetta
Rosa
R
Parrilli
Ermenegilda
E
Parrilli
Michelangelo
M
Tutino
M Luisa
ML
Ummarino
Salvatore
S
D000864:Antarctic Regions; D002236:Carbohydrate Conformation; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000032:analysis*; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010710:Phosphates / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D031161:Pseudoalteromonas / Q000737:chemistry / Q000254:growth & development*; D012623:Seawater / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D008070:Lipopolysaccharides; D010710:Phosphates; D011135:Polysaccharides, Bacterial; C023023:lipid-linked oligosaccharides