title: [The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane].
abstract: OBJECTIVE
  To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane.
  
  
  METHODS
  Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer.
  
  
  RESULTS
  The mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly.
  
  
  CONCLUSIONS
  The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.
authors.affiliation: Institute of Radiation Medicine Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Changzhen
authors.initials: C
authors.lastname: Wang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Cong
authors.forename: Hong
authors.initials: H
authors.lastname: Xian
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Cao
authors.forename: Cunpu
authors.initials: C
authors.lastname: Sun
authors.forename: Ke
authors.initials: K
authors.lastname: Wu
mesh_terms: D000818:Animals; D055590:Electromagnetic Phenomena; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008560:Membrane Fluidity / Q000528:radiation effects*; D008930:Mitochondria, Liver / Q000378:metabolism / Q000528:radiation effects; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats