title: Intestinal intussusception associated with adenovirus infection in Mexican children.
abstract: Formalin-fixed intestinal tissue specimens from 12 Mexican pediatric patients with intussusception were examined for the presence of adenovirus. Four patients (33%) had detectable adenovirus antigen in epithelial cells as determined by using immunohistochemical analysis. Two of the patients with positive immunohistochemical results had antigens in dendritic and mononuclear inflammatory cells, and 3 patients had positive results for species C adenovirus by in situ hybridization using adenovirus species-specific probes (A-F). A real-time polymerase chain reaction assay specific for species C (nonenteric) adenoviruses was used to confirm immunohistochemical results and to amplify adenovirus DNA for sequencing. A sequence similar to that for adenovirus serotype 1 was found in 1 patient, serotype 2 in another, and serotype 6 in a third; in the fourth patient, the sequence was indeterminate between serotypes 2 and 6. The assays used in this study proved useful for the identification of species C adenoviruses in formalin-fixed specimens from Mexican pediatric patients with intussusception.
authors.affiliation: Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G32, Atlanta, GA 30333, USA.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Guarner
authors.forename: Beatriz
authors.initials: B
authors.lastname: de Leon-Bojorge
authors.forename: Eduardo
authors.initials: E
authors.lastname: Lopez-Corella
authors.forename: Tara
authors.initials: T
authors.lastname: Ferebee-Harris
authors.forename: Linda
authors.initials: L
authors.lastname: Gooding
authors.forename: C T
authors.initials: CT
authors.lastname: Garnett
authors.forename: Wun-Ju
authors.initials: WJ
authors.lastname: Shieh
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Dawson
authors.forename: Dean
authors.initials: D
authors.lastname: Erdman
authors.forename: Sherif R
authors.initials: SR
authors.lastname: Zaki
mesh_terms: D000257:Adenoviridae Infections / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D007443:Intussusception / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral