title: Gene expression in sheep skin and wool (hair).
abstract: We sequenced 2939 ESTs from fetal and adult sheep skin. Stages of gestation were picked to coincide with the major events in skin appendage (wool follicle) formation. Clustering analysis generated a nonredundant set of ESTs 2435 strong (83% nonredundant). Approximately 24% of these gave no hit to NCBI build 29 of the human genome, while 35% were tentatively classified by putative function based on BLASTX hits with a p(N) of <10(-4). In addition to bioinformatics analysis of our ESTs and gene mapping, we have generated a large EST spatial expression data set using in situ hybridization. One thousand one hundred forty-two ESTs have been used for in situ localization; about 31% are from adult sheep skin, 39% from late gestation fetal sheep skin, and 30% from midgestation fetal sheep skin. These probes have been used in over 3000 hybridization experiments. In this report, we summarize the results of in situs on adult sheep skin.
authors.affiliation: CSIRO Livestock Industries, St. Lucia, Queensland, Australia. david.adelson@tamu.edu
authors.forename: D L
authors.initials: DL
authors.lastname: Adelson
authors.forename: G R
authors.initials: GR
authors.lastname: Cam
authors.forename: U
authors.initials: U
authors.lastname: DeSilva
authors.forename: I R
authors.initials: IR
authors.lastname: Franklin
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D018859:Hair Follicle / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D017403:In Situ Hybridization; D017422:Sequence Analysis, DNA; D034561:Sheep, Domestic / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D012867:Skin / Q000196:embryology / Q000378:metabolism*; D014935:Wool / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
chemical_list: D018076:DNA, Complementary