[Studies of the relationship of melanocortin receptor 1(MC1R) gene with coat color phenotype in pigs].
Although coat color in pigs has no direct relation with economic traits, it affects economic benefit significantly, coat color selection are widely used in pig breeding and production. PCR-Acc II-RFLP, PCR-BspH I-RFLP and PCR-SSCP were used in combination to analyze genotype at MC1R locus among individuals from 16 full-sib pedigrees and 6 Chinese native breeds including Jinhua, Jiaxing Black, Yushan Black, Leping Spotted, Shanggao Spotted and Shengxian Spotted pig. It was found that the Chinese native pig breeds carry a dominant black allele at MC1R at high frequency, this ED1 allele was suggested to be the major allele controlling black coat color in Chinese native pig breed. In addition, the evidence for a new allele was obtained in Shengxian Spotted pigs by PCR-SSCP analysis. It was reconfirmed from the result of pedigree analysis that ED1 was dominant over EP and e, while EP was incompletely dominant over e.
Deng
Su-Hua
SH
Provincial Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchan 330045, China.
Gao
Jun
J
Ren
Jun
J
Chen
Ke-Fei
KF
Ding
Neng-Shui
NS
Ai
Hua-Shui
HS
Lin
Wan-Hua
WH
Wang
Wen-Jun
WJ
Liu
Bao-Sheng
BS
Lai
Fen-Ju
FJ
Huang
Lu-Sheng
LS
D000483:Alleles; D000818:Animals; D001947:Breeding; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D008297:Male; D010375:Pedigree; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics*; D012880:Skin Pigmentation / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D044102:Receptor, Melanocortin, Type 1; D004247:DNA