[Stimulation of DNA repair in human cells damaged by UV and ionizing radiation with soluble growth factors of photomodified blood].
Exposure of a small skin area of volunteers to UV light in 1 minimal erythemal dose is accompanied by rapid appearance in the circulating blood of soluble factors able to restore proliferation of X-ray-damaged autologous lymphocytes, to decrease frequency of chromosome breaks, and to stimulate unscheduled DNA synthesis. The appearance of such an activity in the blood can be also induced without skin irradiation. For this, one volume of a directly UV-irradiated blood is to be mixed in vitro with 10-fold volumes of intact blood, thus modeling the in vivo situation, when a small amount of transcutaneously UV-irradiated blood mixes with intact blood in the circulation. It has been found that the platelet-derived growth factor and epidermal growth factor, added at physiological concentrations to the culture medium, decrease chromosome break frequency in X-damaged cells.
Zubanova
O I
OI
Institute of Cytology RAS, St. Petersburg. zubanova@mail.cytspb.rssi.ru
Snopov
S A
SA
Mikhel'son
V M
VM
Samoĭlova
K A
KA
D000328:Adult; D004247:DNA / Q000096:biosynthesis* / Q000097:blood; D004260:DNA Repair; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D012995:Solubility; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014965:X-Rays / Q000009:adverse effects*
D006133:Growth Substances; D004247:DNA