Embryo development characteristics in Robertsonian and reciprocal translocations: a comparison of results with non-translocation cases.
The effect of translocations on embryo development was evaluated and results were compared in terms of embryo development with those of embryos obtained from standard intracytoplasmic sperm injection (ICSI) cycles. In 23 translocation carriers with 34 cycles, fertilization, pronuclear morphology scoring (PMS), developmental arrest, cleavage and blastocyst formation were evaluated and compared with embryos obtained from non-translocation cases undergoing ICSI (n = 98 cycles). In 28 cycles, preimplantation genetic diagnosis (PGD) was performed on prezygotes (first and second polar body biopsy for female carriers; n = 3) or on embryos having seven or more blastomeres (blastomere biopsy; n = 25). In six cycles for four couples, probes for translocated chromosomes were not available, so PGD could not be performed. Overall, in translocation cases, a lower fertilization rate, a higher rate of retarded embryo development, and a lower rate of blastocyst formation were observed compared with embryos of non-translocation cases. Fluorescence in-situ hybridization (FISH) analysis showed a 70.9% abnormality rate for reciprocal translocations and 55.0% for Robertsonian translocations respectively. In cases with Robertsonian and reciprocal translocation carriers, the probability of poor embryo development, which may be a result of high segregation abnormalities, may negatively affect the outcome of assisted reproductive techniques. This poor prognosis should also be considered when genetic counselling for translocation is given.
Findikli
N
N
Istanbul Memorial Hospital, Reproductive Endocrinology and ART Unit, Piyalepasa Bulvari, 80270, Okmeydani, Istanbul, Turkey. necatif@hotmail.com
Kahraman
S
S
Kumtepe
Y
Y
Donmez
E
E
Biricik
A
A
Sertyel
S
S
Berkil
H
H
Melil
S
S
D001706:Biopsy; D001755:Blastocyst / Q000502:physiology; D002970:Cleavage Stage, Ovum; D046508:Culture Techniques; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009865:Oocytes; D010062:Ovulation Induction; D019836:Preimplantation Diagnosis; D020554:Sperm Injections, Intracytoplasmic; D020858:Tissue and Organ Harvesting; D014178:Translocation, Genetic