title: Solid-phase microextraction and gas chromatography olfactometry analysis of successively diluted samples. A new approach of the aroma extract dilution analysis applied to the characterization of wine aroma.
abstract: The relationship between the composition and the aroma of the wine can be established by using gas chromatography with olfactometric detection (sniffing or GCO), which combines the chromatographic response with the human nose response. To evaluate the contribution of the odor compounds in wine aroma, we designed a new approach of the aroma extract dilution analysis (AEDA) that lies in the GCO analysis of serially diluted wine samples using headspace solid-phase microextraction (HS-SPME) as the extraction technique. The fiber coating used was Flex divinyl-carboxen-polydimethylsiloxane. The method developed was applied to determine the aromatic composition of a red Grenache wine from Priorat (Spain). The method allows 38 important odorants to be determined in the AEDA study, 30 of them precisely identified. These results are similar to those reported by other studies related to this variety of wine. HS-SPME is a suitable technique to obtain representative extracts of wine aroma with several advantages such as simplicity, speediness, and little sample manipulation.
authors.affiliation: Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Avenida Ramón y Cajal 70, E-43005 Tarragona, Spain. qaenol@fe.urv.es
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Martí
authors.forename: Montserrat
authors.initials: M
authors.lastname: Mestres
authors.forename: Cristina
authors.initials: C
authors.lastname: Sala
authors.forename: Olga
authors.initials: O
authors.lastname: Busto
authors.forename: Josep
authors.initials: J
authors.lastname: Guasch
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009812:Odorants / Q000032:analysis*; D012680:Sensitivity and Specificity; D012903:Smell; D014920:Wine / Q000032:analysis*