title: [Studies on dihydrotestosterone-binding capacity in prostate tissue].
abstract: OBJECTIVE
  To explore the effect of the binding ability of the dihydrotestosterone(DHT) in prostate.
  
  
  METHODS
  Twenty-two normal prostate tissues taken from accident-death corpses without serious diseases, and cytosolic and nuclear fractions were prepared with all the endogenous hormone removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed by adding 3H-DHT.
  
  
  RESULTS
  The average DHT-binding capacity of the DHT-binding protein in prostate was (0.0263 +/- 0.0047) nmol/g wet tissue. The DHT-binding capacities of cytosolic and nuclear fractions were (0.0103 +/- 0.0015) nmol/g wet tissue and (0.0155 +/- 0.0035) nmol/g wet tissue respectively, and the difference between them was very significant(P < 0.01).
  
  
  CONCLUSIONS
  The DHT-binding capacity of the DHT-binding protein in prostate is high and maintaining the high DHT level facilitates the effect of DHT.
authors.affiliation: Department of Urology, Shenzhen People's Hospital, Medical College of Jinan University, Shenzhen, Guangdong 518020, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Jiang
authors.forename: Zhao-Dian
authors.initials: ZD
authors.lastname: Chen
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D013196:Dihydrotestosterone