title: Molecular cloning, genomic organization and developmental expression of the Xenopus laevis hyaluronan synthase 3.
abstract: The content of hyaluronan (HA), a polymer of the extracellular matrix involved in a variety of physiological and pathological processes, depends on the activity of synthetic (HAS) and degrading enzymes. Since HA is also involved in embryogenesis, we have used Xenopus as a model organism because information is available for HAS1 and HAS2, but not for HAS3. We report the sequence of xlHAS3 mRNA, its genomic organization and its expression in adult tissues as well as during embryonic development. Interestingly, evidence from in situ hybridization indicates that xlHAS3 expression is restricted to the developing inner ear and cement gland. In addition, we have correlated the expression pattern of the enzymes involved in HA metabolism with the HA content during development.
authors.affiliation: Dipartimento di Biologia Strutturale e Funzionale, Via J.H. Dunant 3, Varese, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Vigetti
authors.forename: Manuela
authors.initials: M
authors.lastname: Viola
authors.forename: Rosalba
authors.initials: R
authors.lastname: Gornati
authors.forename: Michela
authors.initials: M
authors.lastname: Ori
authors.forename: Irma
authors.initials: I
authors.lastname: Nardi
authors.forename: Alberto
authors.initials: A
authors.lastname: Passi
authors.forename: Giancarlo
authors.initials: G
authors.lastname: De Luca
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D007758:Ear, Inner / Q000196:embryology / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes / Q000235:genetics; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000235:genetics; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D029867:Xenopus Proteins; D006820:Hyaluronic Acid; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; C491342:HAS3 protein, Xenopus; C491096:Has2 protein, Xenopus; D000076002:Hyaluronan Synthases; C456262:Hyal2 protein, Xenopus; D006821:Hyaluronoglucosaminidase