title: Expression of hypoxia-inducible factor 1alpha in human normal, benign, and malignant prostate tissue.
abstract: OBJECTIVE
  To investigate hypoxia-inducible factor 1alpha (HIF-1alpha) protein expression in normal prostates (NP), benign prostatic glandular hyperplasia (BPH), and prostate adenocarcinoma (Pca).
  
  
  METHODS
  HIF-1alpha protein expression was determined by immunohistochemistry in formalin-fixed and paraffin-embedded specimens obtained from 13 cases of NP, 28 cases of BPH, and 34 cases of Pca. In cases of Pca, the relationship between HIF-1alpha protein expression and certain clinicopathological factors, such as clinicopathologic stage and Gleason score, was evaluated.
  
  
  RESULTS
  NP manifested no immunoreactivity, whereas Pca and BPH showed significantly increased HIF-1alpha protein expression. A significantly higher expression was observed in Pca specimens compared with BPH samples. In Pca, no significant relationship between HIF-1alpha protein expression and clinicopathological factors was found.
  
  
  CONCLUSIONS
  Our findings of increased HIF-1alpha protein expression in BPH and Pca specimens suggests the potential role of this protein in BPH and Pca.
authors.affiliation: Department of Urology, Saga Medical School, Saga 849-8501, Japan.
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Du
authors.forename: Chisato
authors.initials: C
authors.lastname: Fujiyama
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Zenjiro
authors.initials: Z
authors.lastname: Masaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004268:DNA-Binding Proteins / Q000032:analysis*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D011467:Prostate / Q000737:chemistry*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000737:chemistry*; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; C497442:HIF1A protein, human; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors