title: Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.
abstract: The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).
authors.affiliation: Composante INSA de l'Unité de Microbiologie et Génétique, UMR 5122 CNRS-UCBL-INSA, Bâtiment A. Lwoff, 10 Rue R. Dubois, 69622 Villeurbanne, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Castang
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Shevchik
authors.forename: Nicole
authors.initials: N
authors.lastname: Hugouvieux-Cotte-Pattat
authors.forename: Pierre
authors.initials: P
authors.lastname: Legrand
authors.forename: Richard
authors.initials: R
authors.lastname: Haser
authors.forename: Patrice
authors.initials: P
authors.lastname: Gouet
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016974:Dickeya chrysanthemi / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006046:Gold / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D006046:Gold; D011133:Polysaccharide-Lyases; C023911:pectate lyase