title: [Establishment of transgenic mice for HRX-EEN fusion gene].
abstract: OBJECTIVE
  To study the biological function of fusion gene HRX-EEN and its role in leukemogenesis, and to provide an ideal animal model for anti-leukemia drug screening.
  
  
  METHODS
  HRX-EEN fusion gene was constructed by use of three different DNA fragments, and it was inserted into hCG transgenic vector. G(0) transgenic mice were obtained by microinjection of the recombined DNA into the pronucleus of zygotes, followed by implantation of the injected zygotes into pseudopregnant mice. The integration of the transgene was tested by PCR and its expression by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The sequence of recombined HRX-EEN gene was confirmed by sequencing. PCR testing revealed a total of 7 G(0) transgenic mice, these mice were then mated with C57 wild type mice. Except mouse No. 35 that died, the others all had their F1 offsprings. From these 6 lines of transgenic mice, HRX-EEN gene was found to be stably expressed in 3 lines by RT-PCR. Up to now, all transgenic mice expressing the fusion gene have no obvious abnormal phenotypes.
  
  
  CONCLUSIONS
  A transgenic mice model in which the HRX-EEN fusion gene can be stably expressed has been established.
authors.affiliation: Department of Medical Genetics, Shanghai Hematology Institute, Shanghai Second Medical University, Shanghai, 200025 PR China.
authors.forename: Yue-ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Qiu-hua
authors.initials: QH
authors.lastname: Huang
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Sai-juan
authors.initials: SJ
authors.lastname: Chen
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhu-gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Lu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008822:Mice, Transgenic; D051788:Myeloid-Lymphoid Leukemia Protein; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C105159:SH3GL1 protein, human; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase; C497423:Kmt2a protein, mouse