title: [Changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue].
abstract: OBJECTIVE
  To investigate the changes of sugar chain structures of alkaline phosphatase (ALP) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (HCC).
  
  
  METHODS
  The binding ratios of ALP from 9 normal liver tissues, 16 hepatoma tissues and 16 noncancerous tissues surrounding hepatoma were analysed by affinity chromatography on various lectin columns including leukoagglutinating phytohemagglutinin (L-PHA), lentil lectin (LCA), Datura stramonium agglutinin (DSA), erythroagglutinating phytohemagglutinin (E-PHA) and Sambucus nigra bark agglutinin (SNA).
  
  
  RESULTS
  The binding ratios of ALP on L-PHA (22.94%+/-5.30%), DSA (55.97%+/-13.72%), LCA (38.16%+/-8.87%), E-PHA (11.56%+/-4.81%) and SNA (69.80%+/-13.71%) in HCC tissues were significantly increased (P<0.01) compared with that in normal liver tissues (L-PHA 5.89%+/-2.75%, DSA 36.20%+/-11.58%, LCA 17.90%+/-6.71%, E-PHA 5.38%+/-2.20%, SNA 57.32%+/-11.27%), respectively. t values between the two groups were 8.94, 3.64, 5.94, 3.62 and 2.32, respectively. L-PHA-binding ratio (25.84%+/-4.67%) of ALP in HCC with invasiveness was significantly higher than that (18.10%+/-3.64%) without invasiveness (t=3.71, P<0.01).
  
  
  CONCLUSIONS
  The changes of ALP sugar chain structures occur in HCC tissue. b1-6 branching sugar chain structure of ALP is related to the invasiveness of HCC.
authors.affiliation: Department of Clinical Laboratory, Hua Shan Hospital, Shanghai 200040, China.
authors.forename: Guo-qian
authors.initials: GQ
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Xu
authors.forename: Wan-zhong
authors.initials: WZ
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.forename: Bing
authors.initials: B
authors.lastname: Su
authors.forename: Hui-qi
authors.initials: HQ
authors.lastname: Liang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D002846:Chromatography, Affinity; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D009361:Neoplasm Invasiveness
chemical_list: D002241:Carbohydrates; D037102:Lectins; D000469:Alkaline Phosphatase