Incorporation of 14C-palmitic acid into phospholipids in the isolated rat uterus. Effect of exogenous ovarian steroids and gonadotrophin-induced ovulation.
Effect of different hormonal stage on incorporation of (1-14C)palmitic acid (PA) into the uterine phospholipids (PL) has been studied. Virgin female Wistar rats were divided into the following groups: ovariectomized 7 and 28 days, untreated; ovariectomized received a single injection of estradiol, ovarietomized received a single injection of progesterone (after estradiol-priming), intact cycling females received pregnant mares serum gonadotrophin, intact cycling females injected human chorionic gonadotrophin after PMSG-priming. Uteri horns were incubated a buffer containing 14C-PA. Lipids were extracted and fractionated using thin-layer chromatography. Estradiol increased PL-content, incorporation of (1-14C)-PA into the PL, while progesterone inhibited E-dependent effect, Gonadotrophin-induced ovulation provoked increase in uterine PL-content and remained a stimulatory signal for incorporation of (1-14C)-PA.
Swiatecka
Jolanta
J
Kliniki Ginekologii Akademii Medycznej w Białymstoku.
Zebrowska
Janina
J
Laudański
Piotr
P
D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods; D019308:Palmitic Acid / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D010743:Phospholipids; D019308:Palmitic Acid; D011374:Progesterone; D004958:Estradiol