title: Effect of a nucleoside/nucleotide-free diet in rat allogenic small intestinal transplantation.
abstract: The aim of this study is to estimate the effect of nucleoside (NS) and nucleotide (NT) on the recipient and graft immune response after rat allogenic small intestinal transplantation. Seven-week-old Lewis rats were randomly assigned to two groups, including the NS/NT free group ( n=6) and the NS/NT supplemented group ( n=6), according to the diet received. The recipient Lewis rats were each given diet for 12 days, and then, on the nineteenth day of gestation, a 2 cm jejunum from the donor fetal Fischer rat was transplanted into the abdominal wall of the recipient rats using a non-vascular anastomotic technique. The recipient rats were killed on day 2 after transplantation, and then the recipient plasma interleukin-2 (IL-2) level was measured. In addition, the histological findings of the graft were analyzed. The IL-2 level of the NS/NT free group was significantly lower than that of the NS/NT supplemented group. In order to determine the grade of rejection, the morphological findings were blindly graded on a scale of 0-4. The mean grade of the NS/NT free group was also significantly lower than that of the NS/NT supplemented group. The NS/NT free diet is therefore considered to have an immunosuppressive effect on rat allogenic small intestinal transplantation based on the recipient plasma IL-2 levels and the histological findings of the grafts.
authors.affiliation: Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582, Fukuoka, Japan. ogita@pedsurg.med.kyushu-u.ac.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Ogita
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Suita
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Taguchi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Toru
authors.initials: T
authors.lastname: Uesugi
mesh_terms: D034861:Abdominal Wall / Q000601:surgery; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D006084:Graft Rejection / Q000473:pathology; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000097:blood; D007413:Intestinal Mucosa / Q000473:pathology; D007583:Jejunum / Q000276:immunology / Q000473:pathology / Q000637:transplantation*; D009705:Nucleosides / Q000008:administration & dosage*; D009711:Nucleotides / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
chemical_list: D000276:Adjuvants, Immunologic; D015415:Biomarkers; D007376:Interleukin-2; D009705:Nucleosides; D009711:Nucleotides