Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression.
The Ets family transcription factor PU.1 is required for development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Mouse bone marrow-derived hematopoietic progenitor cells are programmed to differentiate into mast cells, when the cells are maintained in the presence of pokeweed mitogen-stimulated spleen-conditioned medium. However, by retroviral introduction of PU.1 cDNA, the progenitor cells expressed MHC class II, CD11b, CD11c, and F4/80, and acquired the ability to stimulate T cells. Furthermore, PU.1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1.
Nishiyama
Chiharu
C
Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. chinishi@med.juntendo.ac.jp
Nishiyama
Makoto
M
Ito
Tomonobu
T
Masaki
Shigehiro
S
Maeda
Keiko
K
Masuoka
Nobutaka
N
Yamane
Hisakazu
H
Kitamura
Toshio
T
Ogawa
Hideoki
H
Okumura
Ko
K
D000818:Animals; D017951:Antigen Presentation; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D039481:CD11b Antigen / Q000096:biosynthesis; D039521:CD11c Antigen / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis*; D000099232:Proto-Oncogene Protein Spi-1
D000943:Antigens, Differentiation; D039481:CD11b Antigen; D039521:CD11c Antigen; D018076:DNA, Complementary; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C036763:monocyte-macrophage differentiation antigen