Cell cycle dysregulation induced by cytoplasm of Lactococcus lactis ssp lactis in SNUC2A, a colon cancer cell line.
The anticancer effect of cytoplasmic fraction from Lactococcus lactis ssp. lactis, which had showed strong antiproliferative activity against SNUC2A human colon cancer cell line in the previous study, was investigated. The proliferation of SNUC2A was inhibited by the treatment with cytoplasmic fraction of Lactococcus lactis ssp. lactis in a dose-dependent and partially reversible manner. After exposure to the cytoplasmic fraction of Lc. lactis for 72 h, strong antiproliferative activity was efficiently induced through S-phase accumulation in SNUC2A cells. Analysis of cell cycle regulatory proteins demonstrated that the cytoplasmic fraction enhanced the levels of p21CIP1 and cyclin A, decreased cyclin E protein, and slightly reduced the activity of cyclin-dependent kinase 2 (CDK2).
Kim
Ji Yeon
JY
Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Woo
Hee Jong
HJ
Kim
Young-Suk
YS
Kim
Kyoung Heon
KH
Lee
Hyong Joo
HJ
D016203:CDC2 Protein Kinase / Q000032:analysis; D042846:CDC2-CDC28 Kinases / Q000032:analysis / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis; D002455:Cell Division; D002457:Cell Extracts / Q000494:pharmacology*; D002458:Cell Fractionation; D003110:Colonic Neoplasms / Q000473:pathology*; D019925:Cyclin A / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D051357:Cyclin-Dependent Kinase 2; D050759:Cyclin-Dependent Kinase Inhibitor p21; D050760:Cyclin-Dependent Kinase Inhibitor p27; D016213:Cyclins / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry*; D013294:Lactococcus lactis / Q000648:ultrastructure*; D016196:S Phase; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000032:analysis
D018797:Cell Cycle Proteins; D002457:Cell Extracts; D019925:Cyclin A; D019927:Cyclin E; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D016203:CDC2 Protein Kinase; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2