[Effect of ethylene glycol on antioxidative enzymes and lipid peroxidation activity in erythrocytes].
Investigations were aimed at demonstrating changes in the activity of enzymes forming an antioxidative barrier and oxidative stress parameters in erythrocytes of rats which were administrated ethylene glycol during 4 weeks. Superoxide dismutase activity (CuZn-SOD) was evaluated by the Misra and Fridovich method, catalase (EC1.11.1.6.) by the Beers and Sizer method and malonyl dialdehyde concentration (MDA) with Placer et all were assessed in the obtained material through the evaluation of TBARS compounds concentration. In the 4th week of the experiment a decrease in catalase (9.3 U/gHb to 5.7 U/gHb) and superoxide dismutase (2378 U/gHb to 1759 U/gHb) activity was observed. Malonyl dialdehyde concentration increased from the initial 0.14 mumol/gHb to 0.24 mumol/gHb. The investigations carried out have demonstrated that long-term intoxication with ethylene glycol leads to a constant generation of free radicals (increase of MDA concentration) and gradual exhaustion of the antioxidative system.
Jurczyk
Agnieszka P
AP
Katedry i Zakładu Medycyny Sadowej AM w Lodzi.
Gałecki
Piotr
P
Jankowska
Beata
B
Meissner
Ewa
E
Szram
Stefan
S
Fijałkowski
Paweł
P
Błaszczyk
Jan
J
Kedziora
Józef
J
Smigielski
Janusz
J
D000818:Animals; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D019855:Ethylene Glycol / Q000633:toxicity; D016166:Free Radical Scavengers / Q000097:blood*; D006442:Glycated Hemoglobin / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D013482:Superoxide Dismutase / Q000097:blood*; D013997:Time Factors
D002374:Catalase; D019855:Ethylene Glycol; D016166:Free Radical Scavengers; D006442:Glycated Hemoglobin; D008315:Malondialdehyde; D013482:Superoxide Dismutase