title: [Effect of elastic stress on expression of corebinding factor a 1 mRNA in human periodontal ligament cells in vitro].
abstract: OBJECTIVE
  To investigate the osteoblast-like characteristics of human periodontal ligament cells affected by elastic stress in vitro, and the role of corebinding factor a 1 (cbfa1) in alveolar bone formation during orthodontic tooth movement.
  
  
  METHODS
  Rat dig-labeled cbfa1 cDNA probe was prepared from SD rat osteoblasts cultured in vitro. Human periodontal ligament cells were cultured on the elastic bottom plate and stimulated by elastic stress using mechanical loading system for cultured cells in vitro. The expression of cbfa1 mRNA was detected by in situ hybridization method.
  
  
  RESULTS
  Cbfa1 mRNA express in human periodontal ligament cells stimulated by elastic stress and did not express in normal human periodontal ligament cells.
  
  
  CONCLUSIONS
  It is suggested that elastic stress plays a role in the differentiation process from human periodontal ligament cells to osteoblast-like cells. Cbfa1 is a transcription factor in alveolar bone remodeling during orthodontic tooth movement.
authors.affiliation: Department of Orthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Yin-zhong
authors.initials: YZ
authors.lastname: Duan
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
mesh_terms: D000293:Adolescent; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins / Q000235:genetics*; D004548:Elasticity; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C406688:core binding factor alpha