Plant-dependent active biological containment system for recombinant rhizobacteria.
This work describes the construction of the rhizobacterium Pseudomonas putida strain CS-4, which contains an active biological containment (ABC) system that ensures bacterial suicide in the absence of proline. Maize plants exudate proline, which allows CS-4 to colonize the rhizosphere at a similar level to that of the wild-type strain. However, when the plants are removed, the CS-4 population decreased in bulk soil at a higher rate than the wild-type strain.
van Dillewijn
Pieter
P
Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas, Apdo Correos 419, C/Profesor Albareda 1, E-18008 Granada, Spain.
Vílchez
Susana
S
Paz
José A
JA
Ramos
Juan L
JL
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D015169:Colony Count, Microbial; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D009876:Operon; D018272:Porins / Q000235:genetics / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development*; D011994:Recombinant Proteins / Q000378:metabolism; D012988:Soil Microbiology; D003313:Zea mays / Q000254:growth & development / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D008565:Membrane Proteins; D018272:Porins; C080683:PutA protein, Bacteria; D011994:Recombinant Proteins; D011392:Proline; D001616:beta-Galactosidase