title: Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons.
abstract: In cultured rat hippocampal neurons, overexpression of Homer1a/Vesl-1S, an inducible protein upregulated by seizure or long-term potentiation, caused a reduction of punctate distribution of a postsynaptic protein Homer1c/Vesl-1L, without significant decrease in its total amount. Clusters of F-actin were also decreased. Treatments of cells with BDNF or a proteasome inhibitor, which cause increase in the expression level of endogenous Homer1a, also resulted in the reduction of Homer1c puncta. These results indicate that the accumulation of Homer1a, either exogenously expressed or endogenously induced, caused redistribution and dispersion of postsynaptic clusters of Homer1c and F-actin, suggesting an important role of Homer1a in synaptic remodeling.
authors.affiliation: Department of Biology, Faculty of Science, Graduate School of Science, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Inoue
authors.forename: Naoki
authors.initials: N
authors.lastname: Honkura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kato
authors.forename: Sachie
authors.initials: S
authors.lastname: Ogawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Udo
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inokuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Suchie
authors.initials: S
authors.lastname: Ogawa
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D000072233:Homer Scaffolding Proteins; D017774:Long-Term Potentiation / Q000235:genetics; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism; D009473:Neuronal Plasticity / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000235:genetics / Q000378:metabolism; D013570:Synaptic Membranes / Q000235:genetics / Q000378:metabolism*; D009435:Synaptic Transmission / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000199:Actins; D019208:Brain-Derived Neurotrophic Factor; D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D009097:Multienzyme Complexes; D009479:Neuropeptides; D020033:Protein Isoforms; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex