[The mechanism of Radix Salviae Miltiorrhizae Injection on the inhibition of smooth muscle cell proliferation induced by H2O2].
OBJECTIVE
To observe the effect of Radix Salviae Miltiorrhizae Injection (RSMI) on proliferating cell nuclear angiten (PCNA), NF-kappa B and cell cycle phase of vascular smooth muscle cell(VSMC) induced by H2O2 in vitro.


METHODS
H2O2 was added to the medium for culturing rat aortic VSMC as extraneous reactive oxygen species (ROS). The protein expression of PCNA and NF-kappa B was examined with immunocytochemical technique in VSMC, and cell cycle phase analysis was used to detect proliferation of VSMC.


RESULTS
The result showed that the protein expression of PCNA and NF-kappa B became stonger, the ratio of S phase cells became higher in H2O2 group. RSMI could increase the percentage of the cells in G0/G1 phase, decrease the percentage of the cells in S phase and obviously inhibit the expression of PCNA and NF-kappa B proteins.


CONCLUSIONS
The expression of PCNA and NF-kappa B was inhibited and the transition from G0 phase to S phase was blocked, which may be one of the key mechanism of RSMI on the inhibition of VSMC proliferation induced by H2O2.
Du
Xianhua
X
Yunyang Medical College, Shiyan, Hubei 442000.
Yang
Fangju
F
Wang
Weimin
W
Gong
Yingxia
Y
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis; D010936:Plant Extracts; D010946:Plants, Medicinal / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen