Simultaneous determination of fatty, dicarboxylic and amino acids based on derivatization with isobutyl chloroformate followed by gas chromatography-positive ion chemical ionization mass spectrometry.
Gas chromatography-mass spectrometry (GC-MS) with positive ion chemical ionization (PICI) using isobutane as reagent gas was applied for analysis of isobutoxycarbonyl/isobutyl derivatives of 13 fatty, 6 dicarboxylic and 13 amino acids in a single run. For all investigated compounds (except several amino acids) the quasimolecular ions [MH](+) were registered. Asparagine underwent fragmentation via decarboxylation followed by elimination of OC(4)H(9) ([M-117](+)), whereas serine and tyrosine produced the cluster ions [M+C(4)H(9)OCO](+). Estimated detection limits were 6-250 pg in the total ion current (TIC) mode and 3-10 times lower using the selected-ion monitoring (SIM) mode.
Sobolevsky
Tim G
TG
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1 Building 3, 119992, Moscow, Russia. sobolevsky@environment.chem.msu.ru
Revelsky
Alexander I
AI
Revelsky
Igor A
IA
Miller
Barbara
B
Oriedo
Vincent
V
D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D003998:Dicarboxylic Acids / Q000032:analysis* / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D005561:Formates / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D012996:Solutions
D000596:Amino Acids; D003998:Dicarboxylic Acids; D005227:Fatty Acids; D005561:Formates; D012996:Solutions; C039311:isobutyl chloroformate