EIN3-dependent regulation of plant ethylene hormone signaling by two arabidopsis F box proteins: EBF1 and EBF2.
The plant hormone ethylene regulates a wide range of developmental processes and the response of plants to stress and pathogens. Genetic studies in Arabidopsis led to a partial elucidation of the mechanisms of ethylene action. Ethylene signal transduction initiates with ethylene binding at a family of ethylene receptors and terminates in a transcription cascade involving the EIN3/EIL and ERF families of plant-specific transcription factors. Here, we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors. EBF1 overexpression results in plants insensitive to ethylene. In contrast, plants carrying the ebf1 and ebf2 mutations display a constitutive ethylene response and accumulate the EIN3 protein in the absence of the hormone. Our work places EBF1 and EBF2 within the genetic framework of the ethylene-response pathway and supports a model in which ethylene action depends on EIN3 protein stabilization.
Potuschak
Thomas
T
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084 Cédex, Strasbourg, France.
Lechner
Esther
E
Parmentier
Yves
Y
Yanagisawa
Shuichi
S
Grava
Sandrine
S
Koncz
Csaba
C
Genschik
Pascal
P
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D044783:F-Box Proteins / Q000235:genetics / Q000302:isolation & purification*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins; D011956:Receptors, Cell Surface / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics / Q000302:isolation & purification*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C481044:EBF1 protein, Arabidopsis; C481045:EBF2 protein, Arabidopsis; C119963:EIN2 protein, Arabidopsis; C106987:EIN3 protein, Arabidopsis; D005030:Ethylenes; D044783:F-Box Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C093680:ethylene-responsive element binding protein; C036216:ethylene; D044843:SKP Cullin F-Box Protein Ligases