[Effects of testosterone on GDNF mRNA expression in rat ventral prostate].
OBJECTIVE
To investigate the effects of testosterone on GDNF(glial cell-derived neurotrophic factor) expression in rat ventral prostate.


METHODS
Twenty-four male Sprague-Dawley rats were randomized into 3 groups, group A(n = 8, sham operation, uncastrated controls), group B (n = 8, castrated), group C (n = 8, castrated and given testosterone undecanoate (TU) 50 mg/kg by intramuscular injection). Ventral prostate tissues removed from adult male rats at 3 days after operation were analyzed for the expression of GDNF mRNA by semiquantitative RT-PCR assay.


RESULTS
The ventral prostate tissues shrank remarkably in the castrated rats, and prostatic hyperplasia accurred in those that had received both castration and testosterone undecanoate. GDNF mRNA expressed in the normal rats ventral prostates, and the prostatic expression of GDNF mRNA decreased significantly at 3 days after castration(P < 0.05), but increased significantly (P < 0.05) in those that had received both castration and TU.


CONCLUSIONS
The gene expression of glial cell-derived neurotrophic factor in the ventral prostate of rats is dependent on testosterone, and GDNF is involved in the growth of rat ventral prostate.
Zheng
Xiang-Yi
XY
Department of Urology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310003, China.
Xie
Li-Ping
LP
Qin
Jie
J
Chen
Zhao-Dian
ZD
D000818:Animals; D002369:Castration; D015870:Gene Expression / Q000187:drug effects; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology
D051100:Glial Cell Line-Derived Neurotrophic Factor; D012333:RNA, Messenger; D013739:Testosterone; C010792:testosterone undecanoate