title: Release of peptide YY by fat in the proximal but not distal gut depends on an atropine-sensitive cholinergic pathway.
abstract: Peptide YY (PYY) is released when PYY cells in short term culture are exposed to fat suggesting that this peptide may be released by fat in the distal gut without neural stimulation. PYY is also released by fat in the proximal 1/2 of small intestine. To test the hypothesis that the release of PYY by fat in the proximal but not distal intestine may depend on an atropine-sensitive, cholinergic pathway, PYY levels were compared in four dogs equipped with duodenal and mid-intestinal fistulas when 60 mM oleate was perfused into either the proximal (between fistulas) or distal (beyond mid-intestinal fistula) 1/2 of gut at 2 ml/min for 120 min with intravenous administration of saline or atropine. We found that, when fat was confined to the proximal 1/2 of the intestine, PYY release was reduced following intravenous atropine when compared with saline (p<0.01). However, when fat was confined to the distal 1/2 of the intestine, PYY release was not affected by the intravenous atropine. We conclude that PYY release by fat in the proximal but not distal intestine depends on an atropine-sensitive, cholinergic pathway.
authors.affiliation: GI Motility Program and Section of Nutrition, Department of Medicine, Cedars-Sinai Medical Center, CSMC Burns and Allen Research Institute, Los Angeles, CA 90048, USA. henry.lin@cshs.org
authors.forename: Henry C
authors.initials: HC
authors.lastname: Lin
authors.forename: Ian L
authors.initials: IL
authors.lastname: Taylor
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002799:Cholinergic Fibers / Q000378:metabolism*; D004064:Digestive System; D004285:Dogs; D007275:Injections, Intravenous; D007412:Intestinal Fistula; D007421:Intestine, Small / Q000166:cytology* / Q000378:metabolism; D019301:Oleic Acid / Q000008:administration & dosage / Q000494:pharmacology; D019894:Peptide YY / Q000097:blood / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D019894:Peptide YY; D019301:Oleic Acid; D012965:Sodium Chloride; D001285:Atropine