Using the Ras Recruitment System to identify PP2A-B55-interacting proteins.
The RRS system facilitated the discovery of hitherto unknown interactions with the PP2A-B55 subunit. The advantages of the system lie in its ability to identify interactions that may not be detected by traditional yeast two-hybrid systems. The RRS can thus provide a complementary genetic approach to the identification of protein-protein interactions.
Barr
Haim M
HM
Gonda Center for Molecular Biology, B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O. Box 9649, Bat-Galim, Haifa, 31096, Israel.
Sharf
Rakefet
R
Kleinberger
Tamar
T
D001665:Binding Sites; D002455:Cell Division; D007202:Indicators and Reagents; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D018631:ras Proteins
D007202:Indicators and Reagents; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D010749:Phosphoprotein Phosphatases; D018631:ras Proteins