Coronavirus-induced demyelination occurs in the absence of CD28 costimulatory signals.
Infection of mice with mouse hepatitis virus (MHV) strain A59 results in acute encephalitis, hepatitis, and chronic demyelinating disease. T lymphocytes play an important role in MHV infection, and costimulatory signals are an important component of T cell function. To elucidate the role of the main costimulatory molecule, CD28, in MHV pathogenesis and demyelination, we examined the kinetics of MHV-A59 infection in CD28 knockout mice. MHV-A59-infected CD28 knockout mice developed acute encephalitis and hepatitis, and the same degree of chronic demyelination as normal C57Bl/6 (B6) mice. Thus, CD28, the costimulatory T cell molecule, is not required for MHV infection and MHV-induced demyelination.
Gonzales
Donna M
DM
Division of Neuropathology, Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, 613 Stellar-Chance Laboratory, 422 Curie Boulevard, Philadelphia, PA 19104-6100, USA.
Fu
Li
L
Li
Yun
Y
Das Sarma
Jayasri
J
Lavi
Ehud
E
D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000821:virology; D018106:CD28 Antigens / Q000235:genetics* / Q000502:physiology; D017934:Coronavirus / Q000276:immunology*; D018352:Coronavirus Infections / Q000235:genetics / Q000276:immunology*; D003711:Demyelinating Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D018106:CD28 Antigens