Notes on theory and experimental conditions behind two-photon excitation microscopy.
This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.
Esposito
Alessandro
A
Department of Physics, University of Genoa, 16146 Genoa, Italy.
Federici
Federico
F
Usai
Cesare
C
Cannone
Fabio
F
Chirico
Giuseppe
G
Collini
Maddalena
M
Diaspro
Alberto
A
D004247:DNA / Q000648:ultrastructure*; D049673:History, 20th Century; D018613:Microscopy, Confocal / Q000266:history / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000266:history / Q000379:methods*; D008962:Models, Theoretical; D017785:Photons; D012680:Sensitivity and Specificity; D014584:User-Computer Interface
D004247:DNA