Reversed-phase liquid chromatographic-mass spectrometric determination of microcystin-LR in cyanobacteria blooms under alkaline conditions.
Reversed-phase HPLC coupled to the atmospheric pressure ionization-electrospray ionization (API-ESI) MS was used for microcystin-LR detection and quantitation in samples of dried Microcystis aeruginosa cells. An alkaline linear gradient (20 mmol/l ammonium hydroxide-acetonitrile, pH 9.7) was used for elution of the toxic peptides. Limit of detection was 1 microg/ml (20 ng per injection) in the scan mode of MS and 0.1 microg/ml (2 ng per injection) in the case of selective ion monitoring.
Ruangyuttikarn
Werawan
W
Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Miksik
Ivan
I
Pekkoh
Jeeraporn
J
Peerapornpisal
Yuwadee
Y
Deyl
Zdenek
Z
D000468:Alkalies; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins; D013058:Mass Spectrometry; D052998:Microcystins; D010455:Peptides / Q000032:analysis; D010456:Peptides, Cyclic / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000468:Alkalies; D008387:Marine Toxins; D052998:Microcystins; D010455:Peptides; D010456:Peptides, Cyclic; C057862:cyanoginosin LR