Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase.
A truncated version of the spinosyn polyketide synthase comprising the loading module and the first four extension modules fused to the erythromycin thioesterase domain was expressed in Saccharopolyspora erythraea. A novel pentaketide lactone product was isolated, identifying cryptic steps of spinosyn biosynthesis and indicating the potential of this approach for the biosynthetic engineering of spinosyn analogues. A pathway for the formation of the tetracyclic spinosyn aglycone is proposed.
Martin
Christine J
CJ
Biotica Technology Ltd, Building 5, Chesterford Research Park, Little Chesterford, Nr Saffron Walden, Essex, UK CB10 1XL. Christine.Martin@biotica.co.uk
Timoney
Máire C
MC
Sheridan
Rose M
RM
Kendrew
Steven G
SG
Wilkinson
Barrie
B
Staunton
James C
JC
Leadlay
Peter F
PF
D000595:Amino Acid Sequence; D018942:Macrolides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D016930:Saccharopolyspora / Q000235:genetics*; D016415:Sequence Alignment
D018942:Macrolides; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C114883:spinosyn A; C114884:spinosyn D