Crystal structure of hemoglobin from the maned wolf (Chrysocyon brachyurus) using synchrotron radiation.
Crystal structure of hemoglobin isolated from the Brazilian maned wolf (Chrysocyon brachyurus) was determined using standard molecular replacement technique and refined using maximum-likelihood and simulated annealing protocols to 1.87A resolution. Structural and functional comparisons between hemoglobins from the Chrysocyon brachyurus and Homo sapiens are discussed, in order to provide further insights in the comparative biochemistry of vertebrate hemoglobins.
Fadel
Valmir
V
Departamento de Física-IBILCE-UNESP. CEP 15054-000, Sao José do Rio Preto, SP, Brazil.
Canduri
Fernanda
F
Olivieri
Johnny R
JR
Smarra
André L S
AL
Colombo
Marcio F
MF
Bonilla-Rodriguez
Gustavo O
GO
de Azevedo
Walter F
WF
D000818:Animals; D001938:Brazil; D003460:Crystallization; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D006639:Histidine; D007501:Iron; D010108:Oxyhemoglobins / Q000737:chemistry; D011487:Protein Conformation; D017356:Synchrotrons; D019596:Wolves; D014961:X-Ray Diffraction
D006454:Hemoglobins; D010108:Oxyhemoglobins; D006639:Histidine; D007501:Iron