title: Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons. Modulation during neuronal apoptosis.
abstract: Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC1 for Rb-binding site, removing HDAC1 from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis.
authors.affiliation: Istituto di Neurobiologia e Medicina Molecolare, CNR, Viale Marx 43, 00137 Rome, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Barbato
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Corbi
authors.forename: Nadia
authors.initials: N
authors.lastname: Canu
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fanciulli
authors.forename: Annalucia
authors.initials: A
authors.lastname: Serafino
authors.forename: MariaTeresa
authors.initials: M
authors.lastname: Ciotti
authors.forename: Valentina
authors.initials: V
authors.lastname: Libri
authors.forename: Tiziana
authors.initials: T
authors.lastname: Bruno
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Amadoro
authors.forename: Roberta
authors.initials: R
authors.lastname: De Angelis
authors.forename: Pietro
authors.initials: P
authors.lastname: Calissano
authors.forename: Claudio
authors.initials: C
authors.lastname: Passananti
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002531:Cerebellum / Q000201:enzymology / Q000378:metabolism; D000071877:Checkpoint Kinase 1; D002522:Chlorocebus aethiops; D004285:Dogs; D006801:Humans; D009154:Mutation; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016160:Retinoblastoma Protein / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016160:Retinoblastoma Protein; D016875:tau Proteins; D011494:Protein Kinases; D000071877:Checkpoint Kinase 1