title: Segmental flexibility and avidity of IgM in the interaction of polyvalent antigens.
abstract: We prepared IgG and IgM with identical combining sites to a hapten, (4-hydroxy-3-nitrophenyl)acetic acid (NP), and used surface plasmon resonance to evaluate the association constants (Ka) in interactions of these antibodies (Abs) with antigens (Ags) which differed in the size of carriers and NP valence as well as in the stoichiometry of Ag to Ab in the immune complexes. It was found that IgM was unable to form an Ag1Ab1 complex with the highly haptenated Ag, NP(18.6)-bovine serum albumin (BSA), such that one NP(18.6)-BSA molecule was held by multiple contacts with Fab arms from five subunits, although IgM was capable of forming an Ag4Ab1 complex in which each subunit was bound to one NP(18.6)-BSA molecule. IgM was superior to IgG in interactions with large Ags of low hapten density. The Ka values of IgM to these Ags were estimated to be approximately 1x10(9) M(-1), about 20-fold higher than those of IgG. Reduction of inter-subunit and inter-chain disulfide bonds resulted in a decrease in Ka values to large Ags but no change in those to small Ags.
authors.affiliation: Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.
authors.forename: Toranosuke
authors.initials: T
authors.lastname: Tobita
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oda
authors.forename: Takachika
authors.initials: T
authors.lastname: Azuma
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D006241:Haptens / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates / Q000276:immunology; D011485:Protein Binding / Q000276:immunology
chemical_list: D000941:Antigens; D006241:Haptens; D007075:Immunoglobulin M; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid