Immobilization of phospholipase A2 on porous glass and its application for lowering serum cholesterol concentration.
Phospholipase A2 (PLA2; EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA2 from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50 degrees C, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.
Teke
Mustafa
M
Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey. mustafateke@mail.ege.edu.tr
Onal
Seçil
S
Kilinç
Ali
A
Telefoncu
Azmi
A
D000818:Animals; D002784:Cholesterol / Q000097:blood* / Q000302:isolation & purification; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000627:therapeutic use; D005898:Glass; D006863:Hydrogen-Ion Concentration; D006937:Hypercholesterolemia / Q000628:therapy; D008954:Models, Biological; D010741:Phospholipases A / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D054467:Phospholipases A2; D013552:Swine; D013696:Temperature
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D002784:Cholesterol; D010741:Phospholipases A; D054467:Phospholipases A2