[Effect of laminin on karyotypic variability in cell line of Indian muntjac skin fibroblasts].
The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblasts cell line M and karyotypic variant of this line M' on cultivation on a laminin 2/4 coated surface. In cell line M, cultivated on the laminin-coated surface for 4 and 14 days, and in karyotypic variant M', cultivated for 2, 4 and 14 days, the character of cell distribution for the chromosome number has changed. These changes involve a significant decrease in frequency of cells with model numbers of chromosomes, and an increase in frequency of cells with lower chromosome numbers. As a result, new modal chromosome numbers form. The frequency of cells with 4 chromosomes increases significantly; as a rule, such cells are absent in the control cell variants. Many new additional structural variants of the karyotype (SVK) appear. Detachment of cells M' from the laminin-coated surface followed by a 2 day cultivation on a hydrophilic surface, commonly used for routine cell cultivation, does not restore the control cell distribution for chromosomal number. The frequency of chromosomal aberrations on cultivation of the laminin-coated surface does not change relatively to controls. The observed alterations seem to be due to both disturbances of mitotic apparatus and selection of SVK, which are more advantageous to changed culture conditions of the cell population.
Polianskaia
G G
GG
Institute of Gytology RAS, St. Petersburg.
Goriachaia
T S
TS
Pinaev
G P
GP
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects; D002875:Chromosomes / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure; D014644:Genetic Variation; D007621:Karyotyping; D007797:Laminin / Q000494:pharmacology*; D018668:Muntjacs / Q000235:genetics*; D012867:Skin / Q000648:ultrastructure*
D007797:Laminin