Imparting temperature sensitivity and attenuation in ferrets to A/Puerto Rico/8/34 influenza virus by transferring the genetic signature for temperature sensitivity from cold-adapted A/Ann Arbor/6/60.
The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced ts loci exhibited both ts and att phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.
Jin
Hong
H
MedImmune Vaccines, Inc., Mountain View, California 94043, USA. jinh@medimmune.com
Zhou
Helen
H
Lu
Bin
B
Kemble
George
G
D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D004195:Disease Models, Animal; D005289:Ferrets; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000821:virology; D009154:Mutation; D011995:Recombination, Genetic; D012137:Respiratory System / Q000821:virology*; D013696:Temperature; D014613:Vaccines, Attenuated; D014764:Viral Proteins / Q000235:genetics*
D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1