Chlamydia pneumoniae induces the expression of inhibitor of apoptosis 2 (c-IAP2) in a human monocytic cell line by an NF-kappaB-dependent pathway.
Members of the inhibitor of apoptosis protein family are important factors that regulate apoptotic cell death. As demonstrated both by RT-PCR and Western Blot analysis C. pneumoniae infection of the human monocytic cell line Mono Mac 6 induces the expression of mRNA and protein of the cellular inhibitor of apoptosis 2 (c-IAP2). Blocking NF-kappaB DNA-binding activity by the proteasome inhibitor MG-132 results in decrease of C. pneumoniae-induced c-IAP2 expression. Therefore, C. pneumoniae may exploit the NF-kappaB pathway to induce expression of an antiapoptotic host cell protein that may contribute to intracellular survival of the pathogen.
Wahl
Christian
C
Department of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany. christian.wahl@medizin.uni-ulm.de
Maier
Sonja
S
Marre
Reinhard
R
Essig
Andreas
A
D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002690:Chlamydia Infections / Q000235:genetics / Q000378:metabolism*; D016993:Chlamydophila pneumoniae; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D016328:NF-kappa B; D011506:Proteins; D012333:RNA, Messenger; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde