Synthesis of molecularly imprinted polymer with 7-chloroethyl-theophylline-immobilized silica gel as template and its molecular recognition function.
By reaction of 7-chloroethyl-theophylline with aminopropylsilanized silica gel we synthesized a 7-chloroethyl-theophylline-immobilized silica gel as template molecule and prepared a molecularly imprinted polymer (MIP-Si), which had special recognition sites to 7-chloroethyl-theophylline. A conventional molecularly imprinted polymer (MIP) using 7-chloroethyl-theophylline as template was also prepared for comparison. Binding abilities to 7-chloroethyl-theophylline and its structural analogs revealed that the MIP-Si shows much higher binding speed and much more binding capacity than the MIP does.
Zhang
Yuhui
Y
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Tong
Aijun
A
Li
Longdi
L
D019281:Dimerization; D005456:Fluorescent Dyes / Q000494:pharmacology; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D013806:Theophylline / Q000031:analogs & derivatives* / Q000737:chemistry*; D013997:Time Factors; D014466:Ultraviolet Rays
D005456:Fluorescent Dyes; D011108:Polymers; D013806:Theophylline; C057232:7-(2-chloroethyl)theophylline; D012825:Silicon