Convergent responses of Barrington's nucleus neurons to pelvic visceral stimuli in the rat: a juxtacellular labelling study.
Barrington's nucleus impacts on bladder and distal colon function and relays pelvic visceral information to the forebrain. This study investigated processing of information from the bladder and the distal colon by Barrington's nucleus in the rat. The responses of individual Barrington's nucleus neurons to bladder and/or colon distention were characterized using extracellular recording and the recorded neurons were identified using juxtacellular labelling. Most neurons within Barrington's nucleus (79%) were activated by bladder distention, consistent with its role as a pontine micturition centre. Although no neurons were selectively responsive to colon distention, the majority of bladder-responsive neurons (73%) were also activated by colon distention. In a second study, Barrington's nucleus neurons were characterized with respect to their response to colon distention and their immunoreactivity for the stress-related neuropeptide corticotropin-releasing factor (CRF). Of 30 labelled neurons in the central part of Barrington's nucleus, 53% were activated by colon distention and 63% of these were CRF-ir. This is the first report demonstrating that Barrington's nucleus neurons are responsive to colon distention. The results provide evidence for convergence of information from the bladder and the colon onto individual Barrington's nucleus neurons. Taken with evidence that many Barrington's nucleus neurons are synaptically linked to the bladder and colon, the present study suggests a role for these neurons in coordinating peripheral parasympathetic and central responses to both viscera and implicate CRF as a neurotransmitter in this function. Dysfunctions in this circuit may underlie the coexistence of colon and bladder symptoms observed in functional bowel disorders.
Rouzade-Dominguez
Marie-Laure
ML
The Children's Hospital of Philadelphia, 34th and Civic Center Blvd, 402 Abramson Pediatric Research Center, Philadelphia, PA 19104, USA.
Pernar
Luise
L
Beck
Sheryl
S
Valentino
Rita J
RJ
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives*; D048429:Cell Size / Q000502:physiology; D003106:Colon / Q000294:innervation* / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D003672:Defecation / Q000502:physiology; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D007001:Hypogastric Plexus / Q000166:cytology / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology; D011149:Pons / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000502:physiology; D001743:Urinary Bladder / Q000294:innervation* / Q000502:physiology; D014554:Urination / Q000502:physiology; D017833:Visceral Afferents / Q000166:cytology* / Q000502:physiology
C070614:neurobiotin; D001710:Biotin; D003346:Corticotropin-Releasing Hormone