Prediction of the structure and function of AstA and AstB, the first two enzymes of the arginine succinyltransferase pathway of arginine catabolism.
Arginine succinyltransferase and succinylarginine dihydrolase catalyze the first two steps of arginine catabolism by the arginine succinyltransferase pathway. This route is the only major arginine catabolic pathway in Escherichia coli including its pathogenic strains O157 and CFT073. We have used fold recognition tools and identified novel homologies between each of these two enzymes and proteins of known three-dimensional structure: arginine succinyltransferase belongs to the acyl-CoA N-acyltransferase superfamily and succinylarginine dihydrolase belongs to the amidinotransferase superfamily. These findings shed light on the structures, catalytic mechanisms and evolution of diverse enzymes involved in arginine catabolism.
Shirai
Hiroki
H
Department of Biochemistry, University of Cambridge, Old Addenbrooks Site, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
Mizuguchi
Kenji
K
D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000579:Amidinotransferases / Q000235:genetics; D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D001426:Bacterial Proteins; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein
D001426:Bacterial Proteins; D001120:Arginine; D000579:Amidinotransferases; D000217:Acyltransferases; C047082:arginine succinyltransferase; D006867:Hydrolases; C109401:aruB protein, Pseudomonas aeruginosa