title: Expression of mitochondrial HMGCoA synthase and glutaminase in the colonic mucosa is modulated by bacterial species.
abstract: The expression of the colonic mitochondrial 3-hydroxy 3-methyl glutaryl CoA (mHMGCoA) synthase, a key control site of ketogenesis from butyrate, is lower in germ-free (GF) than in conventional (CV) rats. In contrast, the activity of glutaminase is higher. The objective of this study was to investigate whether the intestinal flora can affect gene expression through short chain fatty acid (SCFA) and butyrate production. GF rats were inoculated with a conventional flora (Ino-CV) or with a bacterial strain producing butyrate (Clostridium paraputrificum, Ino-Cp) or not (Bifidobacterium breve, Ino-Bb). In the Ino-CV rats, mHMGCoA synthase expression was restored to the CV values 2 days after the inoculation, i.e. concomitantly with SCFA production. In the Ino-Cp group, but not in the Ino-Bb group, mHMGCoA synthase and glutaminase were expressed at the level observed in the CV rats. These data suggest that the intestinal flora, through butyrate production, could control the expression of colonic mHMGCoA synthase and glutaminase. These modifications in gene expression by butyrate in vivo seem unrelated to a modification of histone acetylation.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France. Claire.cherbuy@jouy.inra.fr
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Claude
authors.initials: C
authors.lastname: Andrieux
authors.forename: Edith
authors.initials: E
authors.lastname: Honvo-Houeto
authors.forename: Muriel
authors.initials: M
authors.lastname: Thomas
authors.forename: Catherine
authors.initials: C
authors.lastname: Ide
authors.forename: Nathalie
authors.initials: N
authors.lastname: Druesne
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaumontet
authors.forename: Béatrice
authors.initials: B
authors.lastname: Darcy-Vrillon
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
mesh_terms: D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D001644:Bifidobacterium / Q000502:physiology*; D002087:Butyrates / Q000378:metabolism; D003013:Clostridium / Q000502:physiology*; D003106:Colon / Q000201:enzymology / Q000528:radiation effects; D017931:DNA Primers; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005720:Gamma Rays; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005972:Glutaminase / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics*; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000382:microbiology / Q000528:radiation effects; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002087:Butyrates; D017931:DNA Primers; D005230:Fatty Acids, Nonesterified; D006657:Histones; D012333:RNA, Messenger; D006904:Hydroxymethylglutaryl-CoA Synthase; D005972:Glutaminase