title: A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor.
abstract: Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster.
authors.affiliation: College of Life Science, Zhejiang University, Hongzhou 310027, China. lyq@m.hz.zj.cn
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Li
authors.forename: Pei-lin
authors.initials: PL
authors.lastname: Chen
authors.forename: Shi-fei
authors.initials: SF
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005809:Genes, Regulator / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D011353:Prodigiosin; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones