Effects of oxidant-induced injury on heme oxygenase and glutathione in cultured aortic endothelial cells from atherosclerosis-susceptible and -resistant Japanese quail.
Recent studies on cultured aortic endothelial cells (AECs) from atherosclerosis-susceptible (SUS) and -resistant (RES) strains of Japanese quail suggest that differences in atherosclerosis susceptibility between RES and SUS may be due to differences in endothelial heme oxygenase (HO) and antioxidant components. We have now investigated the effects of oxidant-induced injury on HO and glutathione (GSH) in AECs from SUS and RES quail. We report that cultured AECs from SUS and RES birds differ in their response to oxidative stress. AECs from the SUS strain cells are more susceptible than those from the RES strain to oxidative stress induced by tert-butylhydroperoxide, as judged by lower HO activity, HO-1 expression, ferritin and GSH levels. Aortic endothelial cells from SUS birds also showed higher levels of catalytic iron, TBARS production and LDH release compared with RES cells, indicating that SUS AECs are more susceptible to oxidative stress than cells from the resistant strain. Furthermore, independently of genetic status, AECs from old birds have higher TBARS and lower levels of HSP70 induction than AECs from younger birds, suggesting that aging is associated with a decreased ability of AECs to respond to oxidative stress, and this may be relevant to the permissive effect of aging on the process of atherogenesis. Our results indicate that genetic factors and endogenous antioxidant systems in the blood vessel wall may be important in determining the susceptibility of vascular cells to oxidative stress and atherosclerotic plaque formation.
Hoekstra
Kenneth A
KA
Faculty of Agricultural Sciences, University of British Columbia, Vancouver, BC, Canada.
Godin
David V
DV
Kurtu
Jamal
J
Cheng
Kimberly M
KM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001011:Aorta / Q000166:cytology* / Q000473:pathology; D001161:Arteriosclerosis / Q000473:pathology*; D001761:Bleomycin / Q000494:pharmacology; D015153:Blotting, Western; D002384:Catalysis; D002460:Cell Line; D002478:Cells, Cultured; D003370:Coturnix; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000096:biosynthesis; D020022:Genetic Predisposition to Disease; D005978:Glutathione / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006335:Heart Injuries / Q000473:pathology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D007770:L-Lactate Dehydrogenase; D015227:Lipid Peroxidation; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D001761:Bleomycin; D005293:Ferritins; D007501:Iron; D007770:L-Lactate Dehydrogenase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D005978:Glutathione