Interactive effects of dietary palm oil concentration and water temperature on lipid digestibility in rainbow trout, Oncorhynchus mykiss.
An experiment was conducted to evaluate the interactive effects of dietary crude palm oil (CPO) concentration and water temperature on lipid and FA digestibility in rainbow trout. Four isolipidic diets with 0, 5, 10, or 20% (w/w) CPO, at the expense of fish oil, were formulated and fed to groups of trout maintained at water temperatures of 7, 10, or 15 degrees C. The apparent digestibility (AD) of the FA, measured using yttrium oxide as an inert marker, decreased with increasing chain length and increased with increasing unsaturation within each temperature regimen irrespective of CPO level fed to the fish. PUFA of the n-3 series were preferentially absorbed compared to n-6 PUFA in all diet and temperature treatments. Except for a few minor FA, a significant (P < 0.05) interaction between diet and temperature effects on FA digestibility was found. Increasing dietary levels of CPO lead to significant reductions in the AD of saturates and, to a lesser extent, also of the other FA. Lowering water temperature reduced total saturated FA digestibility in trout regardless of CPO level. Based on the lipid class composition of trout feces, this reduction in AD of saturates was due in part to the increasing resistance of dietary TAG to digestion. Increasing CPO level and decreasing water temperature significantly increased TAG content in trout fecal lipids, with saturates constituting more than 60% of the FA composition. Total monoene and PUFA digestibilities were not significantly affected by water temperature in fish fed up to 10% CPO in their diet. The potential impact of reduced lipid and FA digestibility in cold-water fish fed diets supplemented with high levels of CPO on fish growth performance requires further research.
Ng
Wing-Keong
WK
Fish Nutrition Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia. wkng@usm.my
Campbell
Patrick J
PJ
Dick
James R
JR
Bell
J Gordon
JG
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004063:Digestion / Q000187:drug effects / Q000502:physiology*; D008055:Lipids / Q000032:analysis / Q000145:classification; D017686:Oncorhynchus mykiss; D000073878:Palm Oil; D010938:Plant Oils / Q000494:pharmacology*; D013696:Temperature; D014867:Water
D004042:Dietary Fats, Unsaturated; D008055:Lipids; D010938:Plant Oils; D014867:Water; D000073878:Palm Oil