title: [Experimental study on constructing small-caliber artery by tissue engineering approach].
abstract: OBJECTIVE
  To investigate the possibility of constructing small-caliber artery by means of tissue engineering.
  
  
  METHODS
  Cell-PGA mixtures were made by separately seeding 1 x 10(7) smooth muscle cells and 5 x 10(6) endothelial cells isolated from neonate umbilicus onto PGA scaffold, the cell-PGA constructs were wrapped around a silicone tube before its implantation subcutaneously to nude mice and the mice were sacrificed in 2 and 6 weeks. The tissue engineered artery (TEA) were examined both grossly and immunohistochemically.
  
  
  RESULTS
  The gross appearance of TEA was similar to that of the natural counterparts; histologic and immunohistochemical analyses of the neoformed tissues revealed a typical artery structure, including the presence of EC at the luminal surface and the presence of SMC and collagen in the wall.
  
  
  CONCLUSIONS
  TEA with histology similar to natural vessel can be constructed by tissue engineering.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Tissue Engineering Research Center of Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan-zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Jin-jun
authors.initials: JJ
authors.lastname: Chen
authors.forename: De-min
authors.initials: DM
authors.lastname: Yin
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhi-cheng
authors.initials: ZC
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Yi-lin
authors.initials: YL
authors.lastname: Cao
mesh_terms: D000818:Animals; D001158:Arteries / Q000601:surgery* / Q000648:ultrastructure; D042783:Endothelial Cells / Q000648:ultrastructure; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*