Differential prevalence of HIV type 1 subtype B and CRF01_AE among different sexual transmission groups in Tokyo, Japan, as revealed by subtype-specific PCR.
We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain re-action (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRFO1_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype Band 12 with CRFO1_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRFO1_AE are evenly predominant in a heterosexually transmitted group.
Kato
Shingo
S
Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. skato@sc.itc.keio.ac.jp
Saito
Rie
R
Hiraishi
Yoshiyuki
Y
Kitamura
Naoto
N
Matsumoto
Tomoko
T
Hanabusa
Hideji
H
Kamakura
Mitsuhiro
M
Ikeda
Yasuo
Y
Negishi
Masayoshi
M
D005260:Female; D015658:HIV Infections / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020010:Heterosexuality; D006716:Homosexuality; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011159:Population Surveillance; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014041:Tokyo