Organic hydroperoxide-induced chemiluminescence of follicular fluid and blood serum samples obtained from women pretreated for in vitro fertilization.
The organic hydroperoxide-induced chemiluminescence of follicular fluid obtained from in vitro fertilized patients and its differently separated fractions were evaluated. Peroxidative stress causes a different photo-emission in the samples which alludes to some factors playing a role in the maintenance of the pro-oxidant/antioxidant balance. Interactions between the protein compounds of the samples and the organic hydroperoxide associate with formation of excited species contributing to the distinctive light emission processes. The technique offers a special re-interpretation of the scavenger state relating to the components of follicular fluid.
Török
A
A
Department of Obstetrics and Gynaecology, County Hospital Pécs, Pécs, Hungary. atorok@elender.hu
Németh
P
P
Török
B
B
Berki
T
T
Tinneberg
H-R
HR
Bódis
J
J
D002478:Cells, Cultured; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008163:Luminescent Measurements; D006080:Ovarian Follicle / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism*
D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide