Substance P immunoreactive nerve fibres in the domestic chick ankle joint before and after acute urate arthritis.
Substance P immunoreactive (SP-IR) nerve fibres were identified using fluorescence immunohistochemistry and the length of the labelled individual nerve fibres was measured using confocal microscopy of whole mounts of the lateral wall of the ankle joint in 2-week-old domestic chicks. In the normal ankle there was an extensive network of SP-IR fibres in the synovial and subsynovial tissue of the joint capsule. Fours hours after injection of sodium urate into the joint space the joint was inflamed and there was a significant reduction in the labelled nerve fibres. These results demonstrate experimentally that painful gouty arthritis produces a neurogenic depletion of substance P from peripheral nerve fibres in the synovial and subsynovial tissue.
Lunam
C A
CA
Department of Anatomy and Histology, School of Medicine, Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia.
Gentle
M J
MJ
D000818:Animals; D018771:Arthralgia / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D001168:Arthritis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D002645:Chickens; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006073:Gout / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism* / Q000473:pathology; D009619:Nociceptors / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013373:Substance P / Q000378:metabolism*; D013583:Synovial Membrane / Q000294:innervation* / Q000473:pathology / Q000503:physiopathology; D013640:Tarsal Joints / Q000294:innervation* / Q000473:pathology / Q000503:physiopathology; D014527:Uric Acid
D014527:Uric Acid; D013373:Substance P