title: Aptamers that recognize the lipid moiety of the antibiotic moenomycin A.
abstract: Moenomycin A is an amphiphilic phosphoglycolipid antibiotic that interferes with the transglycosylation step in peptidoglycan biosynthesis. The antibiotic consists of a branched pentasaccharide moiety, connected to the moenocinol lipid via a glycerophosphate linker. We have previously described the selection of aptamers that require the lipid group and the disaccharide epitopes of the oligosaccharide moiety for moenomycin binding. Here we report that the enriched moenomycin-binding library contains sequences that evolved for specific recognition of the unpolar lipid group of the antibiotic. These results suggest that the evolution of hydrophobic binding pockets in RNA molecules may be much more common than previously assumed.
authors.affiliation: Universität Hamburg, Abteilung Biochemie und Molekularbiologie, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Betat
authors.forename: Stefan
authors.initials: S
authors.lastname: Vogel
authors.forename: Marc
authors.initials: M
authors.lastname: Struhalla
authors.forename: Hans-Heinrich
authors.initials: HH
authors.lastname: Förster
authors.forename: Michael
authors.initials: M
authors.lastname: Famulok
authors.forename: Peter
authors.initials: P
authors.lastname: Welzel
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001455:Bambermycins / Q000096:biosynthesis / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D016384:Consensus Sequence; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D018604:Epitope Mapping; D005994:Glycerophosphates / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008055:Lipids / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000032:analysis / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004187:Disaccharides; D005994:Glycerophosphates; D006017:Glycolipids; D008024:Ligands; D008055:Lipids; D009844:Oligosaccharides; D010761:Phosphorus Radioisotopes; D001455:Bambermycins; C437243:moenomycin A; D012313:RNA