Adhesion molecule expression patterns indicate activation and recruitment of CD4+ T cells from the lymph node to the peripheral blood of early cutaneous leishmaniasis patients.
Adhesion molecules play a crucial role in cell migration and recruitment. Expression of adhesion molecules that preferentially address cells to inflammatory sites is a critical event in the formation and maintenance of leishmaniasis lesions. In this work, we analyzed the expression of CD11a, CD11b and CD62L, adhesion molecules involved in cell activation and circulation, in CD4+ and CD8+ T cells from peripheral blood and lymph nodes of patients with early cutaneous leishmaniasis. The percentage of expression of CD62L, CD11a and CD11b in total lymphocytes was lower in lymph nodes as compared to peripheral blood. Moreover, differences in adhesion molecule expression between blood and lymph nodes were more striking in CD4+ than CD8+ T cells. Stimulation of PBMC from leishmaniasis patients with soluble Leishmania antigens (SLA) lead to the expansion of CD4+CD62Lhigh cells, CD4+CD11b+ cells and to an increase in the intensity of expression of CD11a in CD4+, but not CD8+ T cells. Our data suggest that early activation events that occur in the lymph nodes of patients recently infected with Leishmania lead to changes in T cell adhesion molecule expression, favoring migration to the periphery and increasing the likelihood of further recruitment to lesion sites.
Costa
Ronaldo P
RP
Laboratory of Cell-Cell Interactions, Department of Morphology, C.P. 486. Av. Antônio Carlos, Universidade Federal de Minas Gerais, Biological Sciences Institute, 6627 Pampulha, Belo Horizonte, CEP30161-970, MG, Brazil.
Gollob
Kenneth J
KJ
Machado
Paulo R L
PR
Bacellar
Olívia A R
OA
Almeida
Roque P
RP
Barral
Aldina
A
Barral-Netto
Manoel
M
Carvalho
Edgar M
EM
Dutra
Walderez O
WO
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007891:Leishmania / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000097:blood* / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation
D015703:Antigens, CD; D000953:Antigens, Protozoan; D015815:Cell Adhesion Molecules