title: Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells.
abstract: Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants alpha-lipoic acid and coenzyme Q(10). Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants.
authors.affiliation: Neuroscience Research Center, Medical Faculty (Charité), Humboldt University, Berlin, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ernst
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stolzing
authors.forename: Grit
authors.initials: G
authors.lastname: Sandig
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003067:Coenzymes; D045304:Cytochromes c / Q000032:analysis / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006861:Hydrogen Peroxide / Q000633:toxicity; D015151:Immunoblotting; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010269:Paraquat / Q000633:toxicity; D051381:Rats; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology; D014238:Trichloroacetic Acid / Q000032:analysis / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D003067:Coenzymes; D024101:Mitochondrial Proteins; D014451:Ubiquinone; D014238:Trichloroacetic Acid; D008063:Thioctic Acid; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; C024989:coenzyme Q10; D010269:Paraquat