[Study on the angiogenic ability of tumor fibroblasts].
OBJECTIVE
To explore the role of fibroblasts derived from tumor in the tumor angiogenesis.


METHODS
Two-well co-culture system were used to detect the expression of MMP-9, TGF-beta1, TN and bcl-2 in L929-H22 cells, and their ability of promoting angiogenesis of ECV304 cells and invasion of MDA-MB-231 cells respectively, which were established in our laboratory before. Then their adhesion and the effect of their supernatant on H22 cells proliferation were analysed.


RESULTS
Compared with L929 cells, the adhesion potential of L929-H22 cells increased (F>or=104.32, P<0.001), with the higher level of expression of MMP-9, bcl-2, TN, and TGF-beta1 in L929-H22 cells in creased (t>or=3.3055, P<0.01). L929-H22 and L929 cells enhanced the invasiveness of human mammary cancer MDA-MB-231 cells through artificial basement membrane (Matrigel) 1.21 and 0.48 times respectively (F=266.3, P<0.001). L929-H22 cells induced morphogenesis of ECV304 cells. L929-H22 stimulated endothelial cells to form more and longer tubes than L929 did (F>or=23.75, P<0.01). 25% CM of L929-H22 cells stimulated the growth of H22 cells (F=266.30, P<0.05).


CONCLUSIONS
The results suggested that fibroblasts in tumors secrete more growth factors and angiogenic factors to promote the angiogenesis and invasion of solid tumors.
Zhu
Hong-mei
HM
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Tang
Wei-xue
WX
Zhou
Wei-ping
WP
D000818:Animals; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000098:blood supply*; D009389:Neovascularization, Pathologic / Q000209:etiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis
D019253:Proto-Oncogene Proteins c-bcl-2; D016212:Transforming Growth Factor beta; D020780:Matrix Metalloproteinase 9