title: Reversed-phase liquid chromatographic-mass spectrometric determination of microcystin-LR in cyanobacteria blooms under alkaline conditions.
abstract: Reversed-phase HPLC coupled to the atmospheric pressure ionization-electrospray ionization (API-ESI) MS was used for microcystin-LR detection and quantitation in samples of dried Microcystis aeruginosa cells. An alkaline linear gradient (20 mmol/l ammonium hydroxide-acetonitrile, pH 9.7) was used for elution of the toxic peptides. Limit of detection was 1 microg/ml (20 ng per injection) in the scan mode of MS and 0.1 microg/ml (2 ng per injection) in the case of selective ion monitoring.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Werawan
authors.initials: W
authors.lastname: Ruangyuttikarn
authors.forename: Ivan
authors.initials: I
authors.lastname: Miksik
authors.forename: Jeeraporn
authors.initials: J
authors.lastname: Pekkoh
authors.forename: Yuwadee
authors.initials: Y
authors.lastname: Peerapornpisal
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Deyl
mesh_terms: D000468:Alkalies; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins; D013058:Mass Spectrometry; D052998:Microcystins; D010455:Peptides / Q000032:analysis; D010456:Peptides, Cyclic / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000468:Alkalies; D008387:Marine Toxins; D052998:Microcystins; D010455:Peptides; D010456:Peptides, Cyclic; C057862:cyanoginosin LR