title: Mechanism of chorismate synthase. Role of the two invariant histidine residues in the active site.
abstract: Chorismate synthase catalyzes the last step in the common shikimate pathway leading to aromatic compounds such as the aromatic amino acids. The reaction consists of the 1,4-anti-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate to yield chorismate. Although this reaction does not involve a net redox change, the enzyme has an absolute requirement for reduced flavin mononucleotide, which is not consumed during the reaction. Two invariant histidine residues are found in the active site of the enzyme: His(17) and His(106). Using site-directed mutagenesis, both histidines were replaced by alanine, reducing the activity 10- and 20-fold in the H106A and H17A mutant protein, respectively. Based on the characterization of the two single mutant proteins, it is proposed that His(106) serves to protonate the monoanionic reduced FMN, whereas His(17) protonates the leaving phosphate group of the substrate. An enzymatic reaction mechanism in keeping with the experimental results is presented.
authors.affiliation: Swiss Federal Institute of Technology Zürich, Department of Agricultural and Food Sciences, Universitätstrasse 2, CH-8092 Zürich, Switzerland.
authors.forename: Karina
authors.initials: K
authors.lastname: Kitzing
authors.forename: Sigrid
authors.initials: S
authors.lastname: Auweter
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Amrhein
authors.forename: Peter
authors.initials: P
authors.lastname: Macheroux
mesh_terms: D020134:Catalytic Domain; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006639:Histidine; D007700:Kinetics; D009492:Neurospora crassa / Q000201:enzymology; D010084:Oxidation-Reduction; D019761:Phosphorus-Oxygen Lyases / Q000032:analysis* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D006639:Histidine; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases