cDNA cloning, genomic structure, chromosomal mapping, and expression analysis of parotid secretory protein in pig.
A novel cDNA has been isolated from pig parotid glands by 3' and 5' rapid amplification of cDNA ends and designated parotid secretory protein (PSP). The open reading frame of this cDNA covers 714 bases, encoding 238 amino acids, which show 56% identity with human PSP at the level of the primary protein structure. The PSP genomic sequence comprises eight exons and seven introns, is approximately 22 kb in size, determined by sequencing, and maps to pig chromosome 17q21-q23. RT-PCR, dot blot, and Northern blot analyses demonstrated that PSP is strongly expressed in parotid glands, but is not present in heart, liver, lung, kidney, muscle, or stomach. A search for functionally significant protein motifs revealed consensus sequences for casein kinase II phosphorylation and N-myristoylation. We observed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu-X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is similar to the leucine zipper.
Yin
Hai Fang
HF
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China.
Zhao
Zhi Hui
ZH
Fan
Bao Liang
BL
Liu
Zhao Liang
ZL
Lu
Wei
W
Liu
Yu Fang
YF
Li
Ning
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D020869:Gene Expression Profiling; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism; D017433:Protein Structure, Secondary; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa / Q000235:genetics*; D026801:Synteny
C045623:BPIFA2 protein, human; D018076:DNA, Complementary; D012471:Salivary Proteins and Peptides; D004247:DNA