title: [Studies of the relationship of melanocortin receptor 1(MC1R) gene with coat color phenotype in pigs].
abstract: Although coat color in pigs has no direct relation with economic traits, it affects economic benefit significantly, coat color selection are widely used in pig breeding and production. PCR-Acc II-RFLP, PCR-BspH I-RFLP and PCR-SSCP were used in combination to analyze genotype at MC1R locus among individuals from 16 full-sib pedigrees and 6 Chinese native breeds including Jinhua, Jiaxing Black, Yushan Black, Leping Spotted, Shanggao Spotted and Shengxian Spotted pig. It was found that the Chinese native pig breeds carry a dominant black allele at MC1R at high frequency, this ED1 allele was suggested to be the major allele controlling black coat color in Chinese native pig breed. In addition, the evidence for a new allele was obtained in Shengxian Spotted pigs by PCR-SSCP analysis. It was reconfirmed from the result of pedigree analysis that ED1 was dominant over EP and e, while EP was incompletely dominant over e.
authors.affiliation: Provincial Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchan 330045, China.
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Deng
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Chen
authors.forename: Neng-Shui
authors.initials: NS
authors.lastname: Ding
authors.forename: Hua-Shui
authors.initials: HS
authors.lastname: Ai
authors.forename: Wan-Hua
authors.initials: WH
authors.lastname: Lin
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Wang
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Liu
authors.forename: Fen-Ju
authors.initials: FJ
authors.lastname: Lai
authors.forename: Lu-Sheng
authors.initials: LS
authors.lastname: Huang
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D008297:Male; D010375:Pedigree; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics*; D012880:Skin Pigmentation / Q000235:genetics*; D013552:Swine / Q000235:genetics*
chemical_list: D044102:Receptor, Melanocortin, Type 1; D004247:DNA