Herpes simplex virus type 1 inoculation enhances hippocampal excitability and seizure susceptibility in mice.
Herpes simplex virus type 1 (HSV-1) is the major pathogen related to epilepsy. However, little is known about the pathogenesis of HSV-1-associated epilepsy. Here, we report that corneal inoculation of mice with HSV-1 induces acute spontaneous behavioural and electrophysiological seizures and chronically increases hippocampal excitability and seizure susceptibility. In slices from infected mice, the surviving hippocampal CA3 pyramidal neurons exhibited a more depolarizing resting membrane potential concomitant with an increase in membrane input resistance. They also had a lower threshold for generating synchronized bursts and a decrease in the amplitude of afterhyperpolarization (AHP) than did controls. These results suggest that a direct change in the excitability of the hippocampal CA3 neuronal network could play an important role in facilitating the development of acute seizures and subsequent epilepsy.
Wu
Hung-Ming
HM
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Huang
Chiung-Chun
CC
Chen
Shun-Hua
SH
Liang
Ying-Ching
YC
Tsai
Jing-Jane
JJ
Hsieh
Ching-Liang
CL
Hsu
Kuei-Sen
KS
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D015984:Causality; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D017097:Electric Impedance; D020803:Encephalitis, Herpes Simplex / Q000150:complications* / Q000503:physiopathology; D004827:Epilepsy / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D004833:Epilepsy, Temporal Lobe / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D007608:Kainic Acid; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009434:Neural Pathways / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000276:immunology / Q000473:pathology / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism
D014764:Viral Proteins; D007608:Kainic Acid