title: BAT-25 and BAT-26, two mononucleotide microsatellites, are not sensitive markers of microsatellite instability in acute myeloid leukaemia.
abstract: BAT-25 and BAT-26 are mononucleotide microsatellites with quasi-monomorphic allele length distribution in healthy controls but unstable, shortened alleles in solid organ tumours with a mutator phenotype (RER+). Both markers are highly sensitive and specific for RER+ colorectal cancer. This study evaluated three mononucleotide microsatellites, BAT-25, BAT-26 and BAT-40 (a polymorphic mononucleotide microsatellite) in RER+ acute myeloid leukaemia (AML). Sixteen [six therapy-related AML (t-AML), 10 de novo AML] known RER+ AML, 22 healthy controls and five known RER- AML samples were analysed. In healthy controls and RER- AML, BAT-25 and BAT-26 were quasi-monomorphic and BAT-40 was polymorphic. Of the RER+ AML samples, 0 of 16 had microsatellite instability (MSI) at BAT-25, 0 of 16 had MSI at BAT-26 and three of 16 had MSI at BAT-40. We conclude that BAT-25, BAT-26 and BAT-40 microsatellites are insensitive to RER+ AML. Further studies are required to determine a consensus panel of sensitive microsatellites for use in AML.
authors.affiliation: Division of Haematology, Department of Clinical Laboratory Sciences, University of Nottingham, Clinical Sciences Building, Hucknall Road, Nottingham NG5 1PB, UK. rowena.bainton@nottingham.ac.uk
authors.forename: Rowena D
authors.initials: RD
authors.lastname: Faulkner
authors.forename: Claire H
authors.initials: CH
authors.lastname: Seedhouse
authors.forename: Emma P
authors.initials: EP
authors.lastname: Das-Gupta
authors.forename: Nigel H
authors.initials: NH
authors.lastname: Russell
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D020137:Base Pair Mismatch; D045744:Cell Line, Tumor; D004260:DNA Repair; D005819:Genetic Markers / Q000235:genetics; D006720:Homozygote; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D005819:Genetic Markers