Steroids and adrenomedullin growth patterns in human ovarian cancer cells: estrogenic-regulation assay.
OBJECTIVE
Adrenomedullin is a multifunctional regulatory peptide with mitogenic and angiogenic capabilities. Objectives in this study were: (1) to describe the effects of adrenomedullin and anti-adrenomedullin antibodies on ovarian carcinoma cell proliferation, and (2) to assess the estradiol regulation of adrenomedullin metabolism.


METHODS
We assessed the effects of estradiol, adrenomedullin, and anti-adrenomedullin antibodies on cell growth in three human ovarian cell lines. RT-PCR was used to assess mRNA expression and Western blots to determine protein levels.


RESULTS
Estradiol stimulates BG-1 and PEO4 cells growth but not PEO14 cells. Adrenomedullin mRNA expression and secretion were not under estrogen control. Adrenomedullin and anti-adrenomedullin antibodies had no growth effects in vitro. Adjunction of anti-adrenomedullin antibodies to estradiol-stimulated cells significantly inhibited their growth.


CONCLUSIONS
Adrenomedullin metabolism is not under estradiol control. Anti-adrenomedullin antibodies display inhibitory effects on cells having high mitogenic activity. This opens the need for additional search toward in vivo specific immunotherapy.
Giacalone
Pierre-Ludovic
PL
Department of Obstetrics and Gynecology (Pr F Laffargue), Hôpital Arnaud de Villeneuve, University of Montpellier, Montpellier, France. pl.giacalone@wanadoo.fr
Daurés
J P
JP
Ouafik
L'Houcine
L
Martin
Pierre-Marie
PM
Laffargue
François
F
Maudelonde
Thierry
T
D053607:Adrenomedullin; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D004958:Estradiol / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010455:Peptides / Q000235:genetics / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D000906:Antibodies; D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin; D004958:Estradiol