Determination of isosorbide-5-mononitrate in human plasma by high-resolution gas chromatography.
A method for the determination of isosorbide-5-mononitrate (5-ISMN) in human plasma by capillary gas chromatography with electron-capture detection was developed and applied to clinical samples. 9-Fluorenone was used as an internal standard, ethyl acetate was employed for liquid-liquid extraction. The advantage of the extraction procedure is the possibility of a direct injection of the plasma extract, without solvent removal/reconstitution of the sample. The precision and accuracy of the method were satisfactory in the concentration range 10-1600 ng/ml. The lower limit of quantification was 10 ng/ml.
Pastera
J
J
Institute of Experimental Biopharmaceutics, Joint Research Center of PRO.MED.CS Praha a.s. and Academy of Sciences of the Czech Republic, Czech Republic. pastera@uebf.cas.cz
Vyslouzil
L
L
Kvetina
J
J
D002849:Chromatography, Gas; D004129:Dimethylpolysiloxanes; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D007548:Isosorbide Dinitrate / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012828:Silicones; D012996:Solutions; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
D004129:Dimethylpolysiloxanes; D005449:Fluorenes; D007202:Indicators and Reagents; D012828:Silicones; D012996:Solutions; D014665:Vasodilator Agents; C013830:baysilon; C028401:9-fluorenone; D007548:Isosorbide Dinitrate; C030397:isosorbide-5-mononitrate