Genotype and allele frequencies of polymorphic cytochromes P450 CYP1A2 and CYP2E1 in Mexicans.
CYP1A2 and CYP2E1 are two of the main cytochrome P450 isoforms involved in the metabolism of commonly used drugs and xenobiotic compounds considered to be responsible for or possible participants in the development of several human diseases. Individual susceptibility to developing these pathologies relies, among other factors, on genetic polymorphism which depends on ethnic differences, as the frequency of mutant genotypes varies in different human populations. Thus the aim of this study was to investigate the frequency of CYP1A2 5'-flanking region and CYP2E1 Rsa I/Pst I polymorphisms in Mexicans by PCR-RFLP methods. The DNA of 159 subjects was analysed and mutant allele frequencies of 30% for CYP2E1 Rsa I/Pst I sites and 43% for CYP1A2 5'-flanking region were found. These frequencies are higher than those previously reported for other human populations.
Mendoza-Cantú
Ania
A
Sección de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, San Pedro Zacatenco, México.
Castorena-Torres
Fabiola
F
Bermudez
Mario
M
Martínez-Hernández
Roberto
R
Ortega
Arturo
A
Salinas
Juan E
JE
Albores
Arnulfo
A
D000293:Adolescent; D000328:Adult; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D019392:Cytochrome P-450 CYP2E1; D019388:Cytochrome P-450 CYP1A2