title: Human synaptobrevin-like 1 gene basal transcription is regulated through the interaction of selenocysteine tRNA gene transcription activating factor-zinc finger 143 factors with evolutionary conserved cis-elements.
abstract: The synaptobrevin-like 1 (SYBL1) gene is ubiquitously expressed and codes for an unusual member of the v-SNAREs molecules implicated in cellular exocytosis. This X-linked gene has the peculiarity of also being present on the Y chromosome in a transcriptional inactive status. Moreover, although ubiquitous, the function of SYBL1 is prominent in specific tissues, such as brain. As a first insight into the molecular mechanisms controlling SYBL1 expression, in this report we describe the extent and role of SYBL1 upstream regions and characterize the binding of trans-acting factors. In vivo foot-printing experiments identify three protected regions. Band shift and transient reporter gene assays indicate a strong role of two of these evolutionary conserved regions in regulating SYBL1 transcription. Because one site is the classical CAAT box, we characterized the binding to the other site of the mammalian homologues of the selenocysteine tRNA gene transcription activating factor (Staf) family, zinc-finger transcription factors, and their role in regulating SYBL1 expression. The results reported here clarify that a Staf-zinc finger family factor, together with the CAAT factor, is the major nuclear protein bound to the SYBL1 promoter region and is responsible for its regulation in HeLa cells, thus identifying the basic control of SYBL1 transcription. In vivo binding of Staf proteins to the SYBL1 promoter is confirmed by chromatin immunoprecipitation assays. Our results identify a fourth mRNA promoter stimulated by a member of the Staf-zinc finger family, the function of which on mRNA polymerase II promoters is still very poorly understood.
authors.affiliation: Institute of Genetics and Biophysics A. Buzzati Traverso, Consiglio Nazionale delle Ricerche, 80131 Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Leva
authors.forename: Maria Immacolata
authors.initials: MI
authors.lastname: Ferrante
authors.forename: Francesca
authors.initials: F
authors.lastname: Demarchi
authors.forename: Antonella
authors.initials: A
authors.lastname: Caravelli
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Matarazzo
authors.forename: Mauro
authors.initials: M
authors.lastname: Giacca
authors.forename: Michele
authors.initials: M
authors.lastname: D'Urso
authors.forename: Maurizio
authors.initials: M
authors.lastname: D'Esposito
authors.forename: Annamaria
authors.initials: A
authors.lastname: Franzé
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000235:genetics; D050683:R-SNARE Proteins; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D050683:R-SNARE Proteins; D015534:Trans-Activators; C121241:VAMP7 protein, human; C114253:ZNF143 protein, human; D004247:DNA