A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco.
Hydrogen peroxide plays a central role in launching the defense response during stress in plants. To establish a molecular profile provoked by a sustained increase in hydrogen peroxide levels, catalase-deficient tobacco plants (CAT1AS) were exposed to high light (HL) intensities over a detailed time course. The expression kinetics of >14000 genes were monitored by using transcript profiling technology based on cDNA-amplified fragment length polymorphism. Clustering and sequence analysis of 713 differentially expressed transcript fragments revealed a transcriptional response that mimicked that reported during both biotic and abiotic stresses, including the up-regulation of genes involved in the hypersensitive response, vesicular transport, posttranscriptional processes, biosynthesis of ethylene and jasmonic acid, proteolysis, mitochondrial metabolism, and cell death, and was accompanied by a very rapid up-regulation of several signal transduction components. Expression profiling corroborated by functional experiments showed that HL induced photoinhibition in CAT1AS plants and that a short-term HL exposure of CAT1AS plants triggered an increased tolerance against a subsequent severe oxidative stress.
Vandenabeele
Steven
S
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
Van Der Kelen
Katrien
K
Dat
James
J
Gadjev
Ilya
I
Boonefaes
Tom
T
Morsa
Stijn
S
Rottiers
Pieter
P
Slooten
Luit
L
Van Montagu
Marc
M
Zabeau
Marc
M
Inze
Dirk
D
Van Breusegem
Frank
F
D016923:Cell Death / Q000187:drug effects; D005784:Gene Amplification; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008027:Light; D011110:Polymorphism, Genetic; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic / Q000187:drug effects*
D006861:Hydrogen Peroxide