[Identification and typification of the human papilloma virus in women using the "Timely Detection of Cancer" program in Durango, Mexico].
OBJECTIVE
In Mexico, the cervical carcinoma is a public health problem, representing more of 36% of the neoplasms. This carcinoma has been etiologically associated with the human papillomavirus (HPV) infection and the polymerase chain reaction (PCR) has been considered as one of the most sensitive methods for its detection. The aim of this study was to determine the HPV types by PCR-RFLPs in 111 women attending in the DOC programs in Gomez Palacio, Durango.


METHODS
DNA was extracted from cervix-vaginal samples as previously described and HPV were detectad using the primers MY09 and MY11. Viral type was determined according to analysis RFLPs.


RESULTS
The cito-histopatologic diagnoses showed that 93 women did not present cervical alteration: 91 (91.85%) were negative HPV and two (2.15%) were VPH positive (low risk). In contrast, 18 women showed cervical alterations; 17 (94.44%) were VPH positive (11 of low risk and 6 of high risk) and only one did not present HPV infection.


CONCLUSIONS
The distribution of types HPV observed in Gomez Palacio, Durango is according to previous study. This is essential a estimation in the development of strategies for prevention of cervical carcinoma in our region.
Rodríguez-Reyes
E Rosalba
ER
Hospital General de Zona No. 46, Instituto Mexicano del Seguro Social (IMSS), Gómez Palacio, Durango.
Cerda-Flores
Ricardo M
RM
Solís Ríos
N Patricia
NP
Quiñones Pérez
Juan M
JM
Cortés-Gutiérrez
Elva I
EI
D000328:Adult; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000517:prevention & control* / Q000821:virology*