Neutralizing endogenous IL-6 renders mast cells of the MCT type from lung, but not the MCTC type from skin and lung, susceptible to human recombinant IL-4-induced apoptosis.
Human cord blood-derived mast cells undergo apoptosis upon exposure to recombinant human (rh)IL-4 and become resistant to rhIL-4-induced apoptosis when cultured in the presence of rhIL-6. The current study extends these effects of rhIL-4 to different populations of human mast cells, namely fetal liver-derived mast cells, lung-derived mast cells, and skin-derived mast cells. Endogenous production of IL-6 appears to protect fetal liver-derived mast cells and those of the MC(T) phenotype from rhIL-4-mediated apoptosis, because neutralization of IL-6 renders these mast cells sensitive. In contrast, mast cells of the MC(TC) phenotype from skin and lung were resistant to IL-4-mediated apoptosis, even after neutralization of endogenous IL-6. MC(TC) cells were CD124(low), whereas those of the MC(T) cells were CD124(high). These observations extend the phenotypic differences between MC(T) and MC(TC) types of human mast cells to include different functional responses to IL-4.
Oskeritzian
Carole A
CA
Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA.
Zhao
Wei
W
Pozez
Andrea L
AL
Cohen
Neri M
NM
Grimes
Margaret
M
Schwartz
Lawrence B
LB
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000276:immunology*; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005333:Fetus; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; D015847:Interleukin-4