title: Expression of Dmrt1 in a turtle with temperature-dependent sex determination.
abstract: There is a variety of sex determining mechanisms among vertebrates. Many reptiles possess temperature-dependent sex determination (TSD), in which the incubation temperature of the egg determines the sex of the hatchling. The red-eared slider turtle, Trachemys scripta has often been used as a model system for examining the physiology of TSD. In the current study, the expression of Dmrt1 was examined during TSD in this turtle. Dmrt1 is a putative regulator of sex determination/differentiation and has been identified in a variety of vertebrates, including fishes, amphibians, reptiles, birds, and mammals. Specifically, Dmrt1 has been shown to be up-regulated in a male-specific pattern during embryonic development in many vertebrates. In the current study, the expression patterns of Dmrt1 were examined in the developing adrenal-kidney-gonad complexes of T. scripta during embryonic development. Using a quantitative competitive RT-PCR, Dmrt1 was shown to be up-regulated during the thermosensitive period of sex determination in males. In contrast, levels of Dmrt1 remained low in females throughout the thermosensitive period. These data suggest that the up-regulation of Dmrt1 may play a role in male sex determination/sex differentiation during TSD in T. scripta.
authors.affiliation: Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294-1170, USA.
authors.forename: C
authors.initials: C
authors.lastname: Murdock
authors.forename: T
authors.initials: T
authors.lastname: Wibbels
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005314:Embryonic and Fetal Development; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D019849:Sex Determination Processes; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014426:Turtles / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
chemical_list: C110547:DMRT1 protein; D012333:RNA, Messenger; D014157:Transcription Factors