title: Altered interaction of HDAC5 with GATA-1 during MEL cell differentiation.
abstract: The transcription factor GATA-1 plays a significant role in erythroid differentiation and association with CBP stimulates its activity by acetylation. It is possible that histone deacetylases (HDACs) repress the activity of GATA-1. In the present study, we investigated whether class I and class II HDACs interact with GATA-1 to regulate its function and indeed, GATA-1 is directly associated with HDAC3, HDAC4 and HDAC5. The expression profiling and our previous observation that GATA-2 interacts with members of the HDAC family prompted us to investigate further the biological relevance of the interaction between GATA-1 and HDAC5. Coexpression of HDAC5 suppressed the transcriptional potential of GATA-1. Our results demonstrated that GATA-1 and HDAC5 colocalized to the nucleus of murine erythroleukemia (MEL) cells. Furthermore, a portion of HDAC5 moved to the cytoplasm concomitant with MEL cell erythroid differentiation, which was induced by treatment with N,N'-hexamethylenebisacetamide. These observations support the suggestion that control of the HDAC5 nucleocytoplasmic distribution might be associated with MEL cell differentiation, possibly through regulated GATA-1 transactivation.
authors.affiliation: Department of Molecular Medicine, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watamoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Towatari
authors.forename: Yukiyasu
authors.initials: Y
authors.lastname: Ozawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Miyata
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Okamoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Abe
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Saito
mesh_terms: D000818:Animals; D019556:COS Cells; D002454:Cell Differentiation / Q000502:physiology*; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D006655:Histone Deacetylases / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases