title: [Difference in lymphocyte heat shock protein 71 (HSP71) expression in soldiers of armed police forces from different climatic zones].
abstract: OBJECTIVE
  To analyze the difference between basal and heat-inducible levels of lymphocyte heat shock protein 71 (HSP71) expression in soldiers from Beijing, Zhengzhou and Guangzhou.
  
  
  METHODS
  Flow cytometry and Comet assay were used to detect the level of HSP71 and DNA damage respectively.
  
  
  RESULTS
  Comet assay showed that there was no significant DNA damage before and after heat stress at 41 degrees C for 1 h, and also no difference found among the 3 climatic zones(P > 0.05). HSP71 of all soldiers in the 3 zones elevated after stress (P < 0.05). The basal and heat-inducible levels of HSP71 in Beijing soldiers(845.87 +/- 135.60 and 1254. 47 +/- 239.05 mean fluorescence intensity respectively) were higher than those in Guangzhou soldiers(702.73 +/- 184.70 and 861.72 +/- 225.12 mean fluorescence intensity respectively) (P < 0.05).
  
  
  CONCLUSIONS
  The differences of lymphocyte HSP71 expression before and after heat stress among the soldiers from Beijing, Zhengzhou and Guangzhou suggest that basal and heat-inducible levels of lymphocyte HSP71 expression may be considered as a valuable index to evaluate heat tolerance of soldiers in different climatic zones.
authors.affiliation: Chengdu Wujing Hospital, Chengdu 610000, China.
authors.forename: Huasong
authors.initials: H
authors.lastname: Zeng
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Xiao
authors.forename: Xiujing
authors.initials: X
authors.lastname: Jing
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Ke
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Tan
authors.forename: Fangze
authors.initials: F
authors.lastname: Li
authors.forename: Tangchun
authors.initials: T
authors.lastname: Wu
mesh_terms: D002980:Climate; D020552:Comet Assay; D004249:DNA Damage; D005434:Flow Cytometry; D018882:Heat Stress Disorders / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008889:Military Personnel; D016495:Police
chemical_list: D006360:Heat-Shock Proteins