Oxygen metabolism of the liver during an HA clamp: HV saturation and free radicals.
OBJECTIVE
To clarify changes in the hepatic oxygen metabolism and tissue damage resulting from oxygen-derived free radical generation from polymorphonuclear cells during a hepatic arterial clamp.


METHODS
Subjects were 32 male Wistar rats. Hepatic tissue blood flow, and hepatic venous chemiluminescence, indicating oxygen-derived free radicals from polymorphonuclear cells, and liver lipid peroxide were measured, and hepatic and portal venous blood gas analysis were performed before and after 130 minutes of hepatic arterial clamping.


RESULTS
Hepatic tissue blood flow decreased by hepatic arterial clamp. The values of hepatic arterial oxygen pressure (HTBF), hepatic venous oxygen saturation (ShvO2), and O2 contents after hepatic arterial clamp were lower than those before hepatic arterial clamp (P = 0.035, 0.024, and 0.028, respectively). Hepatic venous chemiluminescence decreased and the lipid peroxide level of the liver increased by hepatic arterial clamp (P = 0.001).


CONCLUSIONS
ShvO2 is useful for the evaluation of hepatic oxygen metabolism and hepatic tissue blood flow during acute hepatic arterial clamp. This condition should prepare the following tissue damage due to oxygen-derived free radicals from polymorphonuclear cells.
Moriwaki
Yoshihiro
Y
Critical Care and Emergency Center, Yokohama City University Medical Center, 4-57 Urafune-cho, Minami-ku, Yokohama, 232-0024, Japan.
Sugiyama
Mitsugi
M
Uchida
Keiji
K
Kosuge
Takayuki
T
Karube
Norihisa
N
Toyoda
Hiroshi
H
Yamamoto
Toshiro
T
Hasegawa
Satoshi
S
Kanaya
Koji
K
Matsuda
Goro
G
Yamagishi
Shigeru
S
Yoshida
Kenichi
K
D000818:Animals; D003250:Constriction; D005609:Free Radicals / Q000097:blood; D000860:Hypoxia / Q000503:physiopathology; D007511:Ischemia / Q000503:physiopathology*; D008054:Lipid Peroxides / Q000097:blood*; D008099:Liver / Q000098:blood supply*; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000276:immunology; D018384:Oxidative Stress / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology
D005609:Free Radicals; D008054:Lipid Peroxides