title: Effect of richlocaine on proliferative activity of osteoblasts and intracellular calcium content in rats.
abstract: We studied the effect of local anesthetic richlocaine on proliferation and intracellular calcium content in cultured osteoblasts from rat parietal bone. In a concentration of 1 mg/ml this drug produced a cytotoxic effect on osteoblasts. In concentrations of 0.01 and 0.001 mg/ml richlocaine in the absence and presence of subtoxic dose of sodium cyanide (0.2 mM) increased the number of osteoblasts by 15.4 and 36.6 or 13.8 and 38.6%, respectively. In a concentration of 1 mg/ml, richlocaine increased the content of cytosolic calcium in osteoblasts by 105%. These effects of richlocaine in low concentrations (0.01 and 0.001 mg/ml) can be related to stimulation of metabolic processes in osteoblasts.
authors.affiliation: Kuban State Medical Academy, Krasnodar; Krasnodar Research Center, Russian Academy of Medical Sciences, Krasnodar Region Administration; Russian State Medical University, Moscow.
authors.forename: A V
authors.initials: AV
authors.lastname: Zadorozhnyi
authors.forename: V L
authors.initials: VL
authors.lastname: Popkov
authors.forename: P A
authors.initials: PA
authors.lastname: Galenko-Yaroshevskii
authors.forename: A V
authors.initials: AV
authors.lastname: Semeikin
mesh_terms: D000779:Anesthetics, Local / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival; D002478:Cells, Cultured; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010294:Parietal Bone / Q000166:cytology; D010880:Piperidines / Q000633:toxicity*; D011042:Poisons / Q000494:pharmacology; D051381:Rats; D012966:Sodium Cyanide / Q000494:pharmacology
chemical_list: D000779:Anesthetics, Local; D010880:Piperidines; D011042:Poisons; C493511:richlocaine; D012966:Sodium Cyanide; D002118:Calcium