title: Immunocompetent hosts as a reservoir of pneumocystis organisms: histological and rt-PCR data demonstrate active replication.
abstract: The present study was conducted to further examine recent data suggesting that pneumocystosis could be transmitted between patients and healthcare workers in the hospital environment, as has been proven with Pneumocystis-infected SCID mice and immunocompetent Balb/c mice. Using an experimental design (i.e., SCID-Balb/c mouse airborne transmission system), the present work found that healthy host-to-healthy host transmission of Pneumocystis organisms can occur, and that 'second' healthy contacts are able to transmit the infectious organisms to immunocompromised hosts. Further tests designed to explore the behavior of Pneumocystis organisms in the lungs of immunocompetent hosts were performed using histological and molecular approaches (e.g. testing the expression of both cyclin-dependent serine-threonine kinase and heat-shock 70 protein in Pneumocystis). The results showed Pneumocystis organisms were able to replicate in the lungs of immunocompetent hosts, which indicates these hosts are a reservoir for Pneumocystis spp.
authors.affiliation: Ecology of Parasitism, Lille Pasteur Institute, 1 rue du Prof-Calmette BP245, 59019, Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Chabé
authors.forename: E
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: C
authors.initials: C
authors.lastname: Creusy
authors.forename: L
authors.initials: L
authors.lastname: Fleurisse
authors.forename: N
authors.initials: N
authors.lastname: Respaldiza
authors.forename: D
authors.initials: D
authors.lastname: Camus
authors.forename: I
authors.initials: I
authors.lastname: Durand-Joly
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000032:analysis; D001707:Biopsy, Needle; D002353:Carrier State; D004271:DNA, Fungal / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D007121:Immunocompetence; D016867:Immunocompromised Host / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009894:Opportunistic Infections / Q000382:microbiology; D045363:Pneumocystis carinii / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000276:immunology / Q000473:pathology / Q000635:transmission*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D000908:Antibodies, Fungal; D004271:DNA, Fungal