title: Glutathione S-transferase P1 has protective effects on cell viability against camptothecin.
abstract: Glutathione S-transferase P1 (GSTP1) is one of the important xenobiotic-metabolizing enzymes. It was reported that GSTP1 was overexpressed in malignant tissues, and its expression level was associated with resistance to chemotherapeutics. We carried out transfection of GSTP1 sense and antisense vectors to examine effects of GSTP1 on cell cycle arrest and apoptosis induced by camptothecin in HeLa cells. Transfection of GSTP1 antisense vector induced apoptosis. Camptothecin-induced S- or G2/M arrest was intensified by transfection of GSTP1 antisense vector, and subsequent apoptosis was attenuated by transfection of GSTP1 sense vector. These results suggest that GSTP1 has protective effects against camptothecin-induced cytotoxicity.
authors.affiliation: Department of Radiology, Yokohama City University School of Medicine, Yokohama City, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Hara
authors.forename: Takeo
authors.initials: T
authors.lastname: Ishii
authors.forename: Mina
authors.initials: M
authors.lastname: Fujishiro
authors.forename: Michiaki
authors.initials: M
authors.lastname: Masuda
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ito
authors.forename: Jun
authors.initials: J
authors.lastname: Nakajima
authors.forename: Tomio
authors.initials: T
authors.lastname: Inoue
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuse
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis; D002166:Camptothecin / Q000494:pharmacology*; D002453:Cell Cycle; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016195:G2 Phase; D005822:Genetic Vectors; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008280:Magnetics; D008938:Mitosis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016196:S Phase; D013997:Time Factors; D014162:Transfection; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016376:Oligonucleotides, Antisense; D015262:Xenobiotics; D004247:DNA; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D002166:Camptothecin