Cu/Zn superoxide dismutase in yeast mitochondria - a general phenomenon.
Fermentative and respiratory yeast strains of genera Saccharomyces, Kluyveromyces, Pichia, Candida and Hansenula have been investigated for mitochondrial localization of Cu/Zn superoxide dismutase (SOD). Pure mitochondrial fractions were obtained and the specific activities of Cu/Zn and Mn SODs were measured in comparison with those in the corresponding cell-free extracts. The Cu/Zn SOD: Mn SOD ratio in mitochondria and crude extracts was calculated and was considered a specific characteristic of all tested strains. Electrophoretical visualization of SOD patterns provided evidence for possible migration of cytosolic Cu/Zn SOD to mitochondria. The characteristic Cu/Zn SOD profile in mitochondria of all tested strains suggested its ubiquity within the fermentative and respiratory yeasts.
Nedeva
Trayana S
TS
Department of General and Industrial Microbiology, Faculty of Biology, The Sofia University St. Kliment Ohridski, Bulgaria.
Petrova
Ventzislava Y
VY
Zamfirova
Daniela R
DR
Stephanova
Elena V
EV
Kujumdzieva
Anna V
AV
D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008928:Mitochondria / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D013482:Superoxide Dismutase