Temperature regulates bacterial protein production: possible role in rosacea.
Facial skin temperature is higher for patients with rosacea. Papules and pustules might arise because bacteria behave differently at these warmer temperatures. We sought to: (1) compare bacteria from facial skin of patients with rosacea with that of control subjects; and (2) grow these bacteria at 30 degrees C and 37 degrees C to compare growth curves and secreted proteins. Bacteria isolated from pustules/skin surfaces of patients with rosacea and skin surfaces of control subjects were identified and cultured at 37 degrees C and 30 degrees C. Secreted proteins were separated by electrophoresis. We found that Staphylococcus epidermidis isolated from patients with rosacea was consistently beta-hemolytic, whereas that from control subjects were nonhemolytic. Bacteria from patients with rosacea grew at the same rate and to the same stationary phase whether cultured at 37 degrees C or 30 degrees C. Isolates from patients with rosacea secreted more proteins, and generally more of each protein at 37 degrees C compared with 30 degrees C. In conclusion, bacteria isolated from patients with rosacea secrete different proteins and different amounts of protein at different temperatures.
Dahl
Mark V
MV
Department of Dermatology, Mayo Clinic Scottsdale, Scottsdale, Arizona, USA.
Ross
Amy J
AJ
Schlievert
Patrick M
PM
D001426:Bacterial Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D006460:Hemolysin Proteins / Q000032:analysis*; D006801:Humans; D012393:Rosacea / Q000503:physiopathology*; D012881:Skin Temperature; D013212:Staphylococcus epidermidis / Q000502:physiology*
D001426:Bacterial Proteins; D006460:Hemolysin Proteins