Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
Ng
Lisa F P
LF
Genome Institute of Singapore, Singapore, Republic of Singapore.
Wong
Michelle
M
Koh
Susie
S
Ooi
Eng-Eong
EE
Tang
King-Fai
KF
Leong
Hoe-Nam
HN
Ling
Ai-Ee
AE
Agathe
Lora V
LV
Tan
Jenny
J
Liu
Edison T
ET
Ren
Ee-Chee
EC
Ng
Lee-Ching
LC
Hibberd
Martin L
ML
D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D014766:Viremia / Q000821:virology*
D012367:RNA, Viral