The NMR-derived solution structure of a new cationic antimicrobial peptide from the skin secretion of the anuran Hyla punctata.
Amphibian skin secretions constitute an important source of molecules for antimicrobial drug research in order to combat the increasing resistance of pathogens to conventional antibiotics. Among the various types of substances secreted by the dermal granular amphibian glands, there is a wide range of peptides and proteins, often displaying potent antimicrobial activities and providing an effective defense system against parasite infection. In the present work, we report the NMR solution structure and the biological activity of a cationic 14-residue amphiphilic alpha-helical polypeptide named Hylaseptin P1 (HSP1), isolated from the skin secretion of the hylid frog Hyla punctata. The peptide antimicrobial activity was verified against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, whereas no significant lytic effect was detected toward red or white blood cells.
Prates
Maura V
MV
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília-DF, Brazil.
Sforça
Maurício L
ML
Regis
Wiliam C B
WC
Leite
José R S A
JR
Silva
Luciano P
LP
Pertinhez
Thelma A
TA
Araújo
Antônio L T
AL
Azevedo
Ricardo B
RB
Spisni
Alberto
A
Bloch
Carlos
C
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology*; D001001:Anura / Q000378:metabolism*; D002176:Candida albicans / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism; D007477:Ions; D007962:Leukocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D011522:Protons; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D013997:Time Factors
D023181:Antimicrobial Cationic Peptides; D007477:Ions; D010455:Peptides; D011522:Protons