Harvesting peripheral blood stem cells from healthy donors on 4th day of cytokine mobilization.
A pilot study was conducted to evaluate the efficacy of harvesting peripheral blood stem cells from normal healthy donors on day 4 after mobilization with G-CSF at 10 microg/kg for 4 days or a sequential combination of GM-CSF at 10 microg/kg for 2 days and G-CSF at 10 microg/kg for 2 more days. Harvesting over the target dose (>4 x 10(6) kg) of CD34+ cells based on the lst leukapheresis performed on day 4 was possible from 7 (53.8%) out of 13 donors. The 7 matched recipients all exhibited early engraftment, except for one who experienced transplant-related mortality, and no differences were observed with the recipients transplanted with stem cells harvested after a 5-6-day growth-factor (GF) treatment. Accordingly, harvesting from normal healthy donors on day 4 after mobilization treatment with a GF was found to be feasible for allogeneic peripheral blood stem cell transplantation and more economical in terms of the cost of the GF and the donor's commitment.
Sohn
Sang Kyun
SK
Department of Hematology/Oncology, Kyungpook National University Hospital, Daegu, South Korea.
Kim
Jong Gwang
JG
Sung
Woo Jin
WJ
Kim
Dong Hwan
DH
Suh
Jang Soo
JS
Lee
Kun Soo
KS
Lee
Kyu Bo
KB
D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000008:administration & dosage*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis; D019520:Living Donors; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D010865:Pilot Projects; D011994:Recombinant Proteins; D013997:Time Factors; D014184:Transplantation, Homologous
D016207:Cytokines; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor