title: Modulation of calcium-dependent chloride secretion by basolateral SK4-like channels in a human bronchial cell line.
abstract: The human bronchial cell line16HBE14o- was used as a model of airway epithelial cells to study the Ca(2+)-dependent Cl(-) secretion and the identity of K(Ca) channels involved in the generation of a favorable driving force for Cl(-) exit. After ionomycin application, a calcium-activated short-circuit current ( I(sc)) developed, presenting a transient peak followed by a plateau phase. Both phases were inhibited to different degrees by NFA, glybenclamide and NPPB but DIDS was only effective on the peak phase. (86)Rb effluxes through both apical and basolateral membranes were stimulated by calcium, blocked by charybdotoxin, clotrimazole and TPA. 1-EBIO, a SK-channel opener, stimulated (86)Rb effluxes. Block of basolateral K(Ca) channels resulted in I(sc) inhibition but, while reduced, I(sc) was still observed if mucosal Cl(-) was lowered. Among SK family members, only SK4 and SK1 mRNAs were detected by RT-PCR. KCNQ1 mRNAs were also identified, but involvement of K(cAMP) channels in Cl(-) secretion was unlikely, since cAMP application had no effect on (86)Rb effluxes. Moreover, chromanol 293B or clofilium, specific inhibitors of KCNQ1 channels, had no effect on cAMP-dependent I(sc). In conclusion, two distinct components of Cl(-) secretion were identified by a pharmacological approach after a Cai2+ rise. K(Ca) channels presenting the pharmacology of SK4 channels are present on both apical and basolateral membranes, but it is the basolateral SK4-like channels that play a major role in calcium-dependent chloride secretion in 16HBE14o- cells.
authors.affiliation: Laboratoire de Physiologie des Membranes cellulaires, Université de Nice-Sophia Antipolis, UMR 6078/CNRS, 06230 Villefranche-sur-Mer, France.
authors.forename: K
authors.initials: K
authors.lastname: Bernard
authors.forename: S
authors.initials: S
authors.lastname: Bogliolo
authors.forename: O
authors.initials: O
authors.lastname: Soriani
authors.forename: J
authors.initials: J
authors.lastname: Ehrenfeld
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D001980:Bronchi / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002713:Chlorine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D006801:Humans; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D015759:Ionomycin / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D024681:Potassium Channels, Calcium-Activated; D020545:Respiratory Mucosa / Q000187:drug effects / Q000502:physiology*; D012413:Rubidium / Q000378:metabolism*
chemical_list: D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496923:KCNN4 protein, human; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D002713:Chlorine; D015759:Ionomycin; D012413:Rubidium; D002118:Calcium