Diagnostic relevance of Langerin detection in cells from bronchoalveolar lavage of patients with pulmonary Langerhans cell histiocytosis, sarcoidosis and idiopathic pulmonary fibrosis.
The diagnosis of pulmonary Langerhans cell histiocytosis might be refined by demonstrating reliability of a new cell marker, i.e., Langerin (CD207), used on bronchoalveolar lavage fluid. For this purpose, we collected material from patients with this disease and also with sarcoidosis and idiopathic pulmonary fibrosis as controls. In addition to the immunocytochemical detection of Langerin, we examined the expression profiles of CD1a and the macrophage tandem-repeat mannose receptor (CD206). To test accessibility of Langerin, a C-type lectin, for mannosides, we employed reverse lectin histochemistry using mannose-containing neoglycoproteins. The analysis revealed a significantly increased percentage of CD1a- and Langerin-positive cells in pulmonary Langerhans cell histiocytosis in comparison with both other studied diseases. No expression of the 175-kDa mannose-binding lectin (CD206) in Langerhans cells was observed. Evidently, binding sites on the cells were not accessible for the mannose-containing neoglycoligand. These results provide evidence for the usefulness of Langerin-directed immuno- and glycohistochemical monitoring of bronchoalveolar lavage fluid in the diagnosis of pulmonary Langerhans cell histiocytosis.
Smetana
Karel
K
Institute of Anatomy, Charles University, 1st Faculty of Medicine, Unemocnice 3, 128 00, Prague 2, Czech Republic. ksmet@lf1.cuni.cz
Mericka
Otakar
O
Saeland
Sem
S
Homolka
Jirí
J
Brabec
Jirí
J
Gabius
Hans-Joachim
HJ
D000328:Adult; D015703:Antigens, CD; D018949:Antigens, CD1 / Q000096:biosynthesis; D000954:Antigens, Surface / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D003937:Diagnosis, Differential; D005260:Female; D006646:Histiocytosis, Langerhans-Cell / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000096:biosynthesis / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011658:Pulmonary Fibrosis / Q000175:diagnosis*; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis*
D015703:Antigens, CD; D018949:Antigens, CD1; D000954:Antigens, Surface; C419393:CD1a antigen; C121916:CD207 protein, human; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface