[Pharmacokinetics of genistein in beagle dogs].
OBJECTIVE
To study the pharmacokinetics of genistein in Beagle dogs.


METHODS
Genistein, suspended in 0.5% CMC-Na solution, was orally administered to Beagle dogs at the dose of 5.34 mg.kg-1. At various time intervals, 1.5 mL of blood was drawn from the vein of dogs in their front legs. At the same time, urine and feces were collected. After the collection, the feces were homogenized with physiological saline (to 1 g feces, 10 mL physiological saline were added). The genistein in plasma, urine and homogenized feces was extracted twice by vortexing with 2.0 mL mixture of methyl tert-butyl ether and pentane (8:2). The organic phase was transferred into tubes and evaporated in ventilation cabinet. The residue was dissolved in 50 microL of methanol and 20 microL of the solution was drawn and detected by high-performance liquid chromatography. The pharmacokinetic parameter was calculated by 3P97 software.


RESULTS
The plasma concentration-time curve was fitted to a one-open-compartment model. The peak time was 0.29 h, and the elimination half-life was 0.52 h. After genistein was administered, 10.79% of genistein were excreted from urine and 21.55% from feces within 24 h. It was also found that 13.00% genistein were excreted from urine and 52.46% from feces within 60 h.


CONCLUSIONS
It showed that the speed of absorption and elimination of genistein was high in Beagle dog, and genistein was mainly excreted in the form of parent compound in urine and feces.
Zhou
Si-yuan
SY
Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China.
Mei
Qi-bing
QB
Yang
Xing-bin
XB
Li
Xin
X
Hu
Yu-zhen
YZ
Wang
Jian-bo
JB
D000818:Animals; D016588:Anticarcinogenic Agents / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005243:Feces / Q000737:chemistry; D019833:Genistein / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
D016588:Anticarcinogenic Agents; D019833:Genistein