title: Quantification of metallothionein-like proteins in the mussel Mytilus galloprovincialis using RP-HPLC fluorescence detection.
abstract: A HPLC-fluorescence method, using the fluorophore SBD-F (ammonium-7-fluorobenz-2-oxa-1,3-diazole-4-sulfonate), was adapted for the quantification of metallothioneins and their isoforms from the Moroccan mussel Mytilus galloprovincialis. The method was first optimized using a rabbit liver metallothionein. The effects of EDTA, tris(2-carboxyethyl)phosphine, and SBD-F on the labeling efficiency were studied. The optimized method was then applied to evaluate the amount of metallothionein in the mussels either exposed to cadmium in the laboratory or collected from the Casablanca coast, Morocco. The concentrations of metallothioneins measured in the field samples describe the degree of contamination of the sites and are reflected by distinct isoform patterns.
authors.affiliation: Martin-Luther-University Halle-Wittenberg, Department Biochemistry/Biotechnology, Division of Ecology and Plant-Biochemistry, Kurt-Mothes-Strasse 3, 06120 Halle, Germany.
authors.forename: Issam
authors.initials: I
authors.lastname: El Ghazi
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Menge
authors.forename: Juergen
authors.initials: J
authors.lastname: Miersch
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Chafik
authors.forename: Ali
authors.initials: A
authors.lastname: Benhra
authors.forename: M Khalid
authors.initials: MK
authors.lastname: Elamrani
authors.forename: Gerd-Joachim
authors.initials: GJ
authors.lastname: Krauss
mesh_terms: D000818:Animals; D049872:Bivalvia; D002104:Cadmium / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D004784:Environmental Monitoring; D005453:Fluorescence; D008668:Metallothionein / Q000032:analysis*; D011817:Rabbits; D014018:Tissue Distribution; D014873:Water Pollutants / Q000633:toxicity
chemical_list: D014873:Water Pollutants; D002104:Cadmium; D008668:Metallothionein