title: Use of design of experiments to optimize high-throughput semipreparative LC and LC/MS methods.
abstract: This paper describes an investigation into decreasing the run time for high-throughput semipreparative RP-HPLC methods without compromising the resolution. Experimental design was used to devise a small set of experiments in which factors, including solvent flow rate, solvent/column temperature, at-column dilution, and run time were varied systematically. The results were analyzed by means of multiple regression and partial least squares to generate a model relating the factors to the results, showing which factors are important. The model was then used to determine the optimal conditions.
authors.affiliation: Chemical & Screening Sciences, Wyeth Research, 401 N. Middletown Road, Pearl River, New York 10965, USA. coledc@wyeth.com
authors.forename: Derek C
authors.initials: DC
authors.lastname: Cole
authors.forename: Nicholas
authors.initials: N
authors.lastname: Pagano
authors.forename: Michael F
authors.initials: MF
authors.lastname: Kelly
authors.forename: John
authors.initials: J
authors.lastname: Ellingboe