Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica.
Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.
Igasaki
Tomohiro
T
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, 305-8687 Japan. iga@ffpri.affrc.go.jp
Akashi
Noriko
N
Ujino-Ihara
Tokuko
T
Matsubayashi
Yoshikatsu
Y
Sakagami
Youji
Y
Shinohara
Kenji
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D032485:Cryptomeria / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D036361:Peptide Hormones; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
D018076:DNA, Complementary; C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011092:Polyethylene Glycols