title: Effect of single dose irradiation on human glioblastoma spheroids in vitro.
abstract: Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.
authors.affiliation: Department of Radiation Oncology, University of Hamburg, Martinistrasse 52, 20246 Hamburg, Germany. fehlauer@uke.uni-hamburg.de
authors.forename: Fabian
authors.initials: F
authors.lastname: Fehlauer
authors.forename: Lukas J A
authors.initials: LJ
authors.lastname: Stalpers
authors.forename: John
authors.initials: J
authors.lastname: Panayiotides
authors.forename: Patricia
authors.initials: P
authors.lastname: Kaaijk
authors.forename: Dionisio
authors.initials: D
authors.lastname: González González
authors.forename: Sieger
authors.initials: S
authors.lastname: Leenstra
authors.forename: Paul
authors.initials: P
authors.lastname: van der Valk
authors.forename: Peter
authors.initials: P
authors.lastname: Sminia
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D005909:Glioblastoma / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D006801:Humans; D009924:Organ Culture Techniques; D011237:Predictive Value of Tests; D016634:Radiosurgery / Q000379:methods*; D011878:Radiotherapy / Q000379:methods; D016019:Survival Analysis; D016212:Transforming Growth Factor beta / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A