title: Infection of human astrocytes and glioblastoma cells with Toxoplasma gondii: monocyte chemotactic protein-1 secretion and chemokine expression in vitro.
abstract: In immunocompromised hosts, disruption of toxoplasmic cysts and conversion from bradyzoites to tachyzoites occur in brain. In these areas, infiltrates of mononuclear cells are observed. In the murine toxoplasmosis model, recent data suggest that chemokines may play a role in leukocyte recruitment in the central nervous system (CNS). This study analyzed the monocyte chemotactic protein-1 (MCP-1) secretion and chemokine expression after Toxoplasma gondii infection of human astrocytes, glioblastoma cells (U373) and fibroblasts (MRC5) in vitro. T. gondii infection of these CNS cells, astrocytes and glioblastoma cells significantly increased MCP-1 secretion, particularly for astrocytes. In our cellular models, the pattern of chemokine gene expression is dominated by MCP-1 expression. MCP-1 mRNAs were also quantified by real-time-PCR (LightCycler). The behavior of cells studied after T. gondii infection was different (invasion and growth) and the cell mechanisms of chemokine regulation could be dependent on the type of cells infected, while MCP-1 may contribute to the cell recruitment during human cerebral reactivation of T. gondii.
authors.affiliation: Interactions Cellulaires Parasite-Hôte, EA UJF 2940, Faculté de Médecine, Université J. Fourier, La Tronche, 38000 Grenoble, France. MPBrenierPinchart@chu-grenoble.fr
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Brenier-Pinchart
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Blanc-Gonnet
authors.forename: Patrice N
authors.initials: PN
authors.lastname: Marche
authors.forename: François
authors.initials: F
authors.lastname: Berger
authors.forename: François
authors.initials: F
authors.lastname: Durand
authors.forename: Pierre
authors.initials: P
authors.lastname: Ambroise-Thomas
authors.forename: Hervé
authors.initials: H
authors.lastname: Pelloux
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000469:parasitology*; D015152:Blotting, Northern / Q000379:methods; D001932:Brain Neoplasms / Q000473:pathology; D002452:Cell Count / Q000379:methods; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000378:metabolism*; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism / Q000469:parasitology; D005909:Glioblastoma / Q000473:pathology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D014122:Toxoplasma / Q000502:physiology*
chemical_list: D018932:Chemokine CCL2; D018925:Chemokines; D012333:RNA, Messenger