[Role of tissue factor in the invasive ability of cultured human colon carcinoma cells].
OBJECTIVE
To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT-29).


METHODS
The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT-29 cells with sense/antisense TFcDNA transfection.


RESULTS
HT-29 cells with sense-TFcDNA transfection showed increased TF expression and invasive ability compared with the cells without transfection, but HT-29 cells with antisense-TFcDNA transfection got the contrary change.


CONCLUSIONS
TF can increase the invasive ability of HT-29 cells in vitro.
Yao
Hongwei
H
Department of General Surgery Peking University First Hospital Beijing 100034 China. docyhw@sina.com
Wan
Yuanlian
Y
Ye
Jingming
J
Wu
Nan
N
Ju
Xiaoming
X
Zhu
Jing
J
D003110:Colonic Neoplasms / Q000473:pathology*; D016373:DNA, Antisense / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000502:physiology*; D014162:Transfection
D016373:DNA, Antisense; D013925:Thromboplastin