title: [Antithrombotic effects of morpholine and piperazine ring derivatives and their molecular mechanism].
abstract: OBJECTIVE
  To investigate the antithrombotic effects of morpholine and piperazine ring derivatives and their mechanisms.
  
  
  METHODS
  In isolated rat aorta-precontracted with norepinephrine (NE), the vasodilatory effects of compounds with novel structure were investigated. Mice was given kappa-carrageenin i.p. and kept at the temperature of (20-21) degree C.
  
  
  CONCLUSIONS
  Active candidate compounds including MOPMC, 2FBMPC, MPTMBC, DMHPPP and PPVP were shown to antagonize thrombosis at the dose of 1 mg.kg-1 through activating the endothelial target for acetylcholine; while the contrasting compounds MAPC, 4C3FBMOC, mTBMPC, MONVP and MPNVP showed no significant effect on the tension of isolated aorta strips or significant antithrombotic effects at the same dose. The antithrombotic mechanism of novel compounds is not relevant to hemostatic systems or functions of platelet aggregation directly, but they can promote endothelial cells to release tissue-type plasminogen activator (t-PA) and inhibit the activity of plasminogen activator inhibitor-1 (PAI-1).
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Chen
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009025:Morpholines / Q000494:pharmacology*; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D005343:Fibrinolytic Agents; D009025:Morpholines; D010879:Piperazines; D017395:Plasminogen Activator Inhibitor 1; D014665:Vasodilator Agents; D000077489:Piperazine; C037574:morpholine; D010959:Tissue Plasminogen Activator