title: Mdm2 mRNA expression in salivary gland tumour cell lines.
abstract: BACKGROUND
  Murine double minute 2 (mdm2) is a cellular protooncogene, which, in conditions of overexpression or amplification, is capable of inactivating the functions of p53, leading to tumorigenesis. Immunoexpression of mdm2 in salivary gland tumours was previously found; however, it was necessary to find out if mdm2 was overexpressed. The aim of this study was to analyse the mRNA expression of mdm2 in salivary gland neoplasms and to correlate it to immunoexpression of p53 and p21 proteins.
  
  
  METHODS
  Specimens of different salivary gland neoplasms were obtained from surgical resections, and cell lineages derived from these tissues were established. RNA extraction was performed and mRNA expression was investigated using reverse transcription-polymerase chain reaction (RT-PCR). Cellular expression of p53 and p21 proteins was investigated by immunofluorescence technique.
  
  
  RESULTS
  Increased expression of mdm2 was found in the majority of cell lines analysed.
  
  
  CONCLUSIONS
  Comparing all results, we postulated that overexpression of mdm2 is related to the tumorigenesis and/or tumour progression of salivary gland neoplasms.
authors.affiliation: Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Lineu Prestes 2227, Cidade Universitária, CEP 05508-900, São Paulo, SP, Brazil. amantesso@hotmail.com
authors.forename: Andrea
authors.initials: A
authors.lastname: Mantesso
authors.forename: Silvia Vanessa Lourenço
authors.initials: SV
authors.lastname: Loducca
authors.forename: Israel
authors.initials: I
authors.lastname: Bendit
authors.forename: Bernardo
authors.initials: B
authors.lastname: Garicochea
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: de Araújo
mesh_terms: D008949:Adenoma, Pleomorphic / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism; D006801:Humans; D009208:Myoepithelioma / Q000378:metabolism; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D051736:Proto-Oncogene Proteins c-mdm2; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)