title: Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development.
abstract: We cloned and characterized a novel member of the tenascin family of extracellular matrix proteins--the murine orthologue of zebrafish tenascin-W. Full-length recombinant tenascin-W was expressed and purified from mammalian cell cultures. Rotary shadowing followed by electron microscopy showed that tenascin-W forms hexabrachions. We studied its expression during development and in the adult by immunohistochemistry, in situ hybridization and immunoblotting. Tenascin-W is expressed during palate formation, osteogenesis and smooth muscle development. In the adult, tenascin-W is found in the kidney, cardiac semilunar valves, corneal limbus and periosteum. Tenascin-W and tenascin-C expression overlap in many of these areas. Bone-morphogenic-protein-2 treated C2C12 cells secrete tenascin-W and are able to adhere to and to extend actin-rich processes on a tenascin-W substratum. In vitro, cells bind to tenascin-W in an RGD-dependent manner. This adhesion is increased by transfection of alpha8 integrin, which localizes with tenascin-W in the periosteum and kidney.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Scherberich
authors.forename: R P
authors.initials: RP
authors.lastname: Tucker
authors.forename: E
authors.initials: E
authors.lastname: Samandari
authors.forename: M
authors.initials: M
authors.lastname: Brown-Luedi
authors.forename: D
authors.initials: D
authors.lastname: Martin
authors.forename: R
authors.initials: R
authors.lastname: Chiquet-Ehrismann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000196:embryology / Q000378:metabolism*; D009928:Organ Specificity; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019063:Tenascin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D019063:Tenascin; D029961:Zebrafish Proteins; C477504:tenascin-N protein, mouse; C112411:tnn protein, zebrafish