title: The effect of dietary lysine deficiency on the immune response to Newcastle disease vaccination in chickens.
abstract: The effect of lysine deficiency on chicken immune function was evaluated using broiler chickens fed a diet with lysine at 67% of the control diet (1.24% lysine). The evaluation of humoral immune function was conducted by measuring the antibody production to a live Newcastle disease virus (NDV) vaccination using the hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA). The cellular immune function was evaluated through the use of cutaneous basophil hypersensitivity test. The antibody response to NDV vaccination was reduced in broiler chickens fed a lysine-deficient diet when measured by ELISA but not when measured by HI. The cell-mediated immune response was also reduced by lysine deficiency.
authors.affiliation: Department of Poultry Science, College of Agriculture, The University of Georgia, Athens, GA 30602, USA.
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: J E
authors.initials: JE
authors.lastname: Sander
authors.forename: N M
authors.initials: NM
authors.lastname: Dale
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology; D002645:Chickens; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D008239:Lysine / Q000172:deficiency*; D009521:Newcastle Disease / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D012016:Reference Values; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines
chemical_list: D014765:Viral Vaccines; D008239:Lysine