title: [An easier method for generating recombinant adenovirus containing CD/TK fusion gene driven by vascular endothelial growth factor promoter].
abstract: OBJECTIVE
  To efficiently construct the recombinant adenovirus containing CD/TK fusion gene driven by vascular endothelial growth factor (VEGF) promoter using improved homologous recombination in bacteria.
  
  
  METHODS
  Chemical transformation, instead of electroporation, of the plasmid pAdEasy-1 into E.coli BJ5183 strain was performed to prepare BJ5183pAdEasy-1 as the competent bacterium, in which pAdEasy-1 and pAdtrack-VEGFP-CDglyTK were recombined. Finally, the recombinant adenovirus of AdVEGFP-CDglyTK was constructed by transfecting 293 cells with linearized adenoviral genomes of pAdEasy-VEGFP-CDglyTK.
  
  
  RESULTS
  This new transformation procedure generated recombinant plasmids in a yield of 60% (6/10), and the adenovirus AdVEGFP-CDglyTK was harvested 7-12 d after transfection.
  
  
  CONCLUSIONS
  The improved homologous recombination in bacteria is efficient, convenient and easy to be carried out.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Huang
authors.forename: Zong-hai
authors.initials: ZH
authors.lastname: Huang
authors.forename: Jian-fa
authors.initials: JF
authors.lastname: Chen
authors.forename: Fu-xiang
authors.initials: FX
authors.lastname: Tang
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Yang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zheng
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Che
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D043525:Cytosine Deaminase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D013937:Thymidine Kinase; D043525:Cytosine Deaminase