title: Regulation of squamous cell carcinoma antigen production by E-cadherin mediated cell-cell adhesion in squamous cell carcinoma cell line.
abstract: Squamous cell carcinoma antigen (SCCA) is a useful tumor marker for diagnosis and management of squamous cell carcinoma. It is well known that cell-cell adhesion is important for progression of cancer. However, it is not clarified whether cell-cell adhesion affects SCCA production in squamous cell carcinoma. The present study was, therefore, undertaken to investigate whether E-cadherin-mediated cell-cell adhesion affects SCCA production in squamous cell carcinoma of the uterine cervix. SKG-IIIa cells or CaSki cells, cervical squamous cell carcinoma cell lines, were treated with anti-E-cadherin antibodies (1 microg/ml) up to 72 h. The cells were dissociated, and SCCA content in the cytosol and SCCA mRNA levels were significantly decreased compared to the control group treated with mouse IgG. Secondly, the signaling pathway for SCCA production mediated by E-cadherin was examined. Phosphatidylinositol (PI) 3-kinase is a well-known mediator of E-cadherin-mediated biological events. The treatment with a PI 3-kinase inhibitor suppressed SCCA production in SKG-IIIa cells. It is concluded that E-cadherin mediated cell-cell adhesion maintains SCCA production through PI 3-kinase in squamous cell carcinoma.
authors.affiliation: Division of Obstetrics and Gynecology, Department of Reproductive Pediatric and Infectious Science, Yamaguchi University School of Medicine, 1-1-1 Minami-kogushi, Ube 755-8505, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Shugo
authors.initials: S
authors.lastname: Nawata
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sueoka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Murakami
authors.forename: Osamu
authors.initials: O
authors.lastname: Takeda
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Numa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sugino
mesh_terms: D000912:Antibodies, Neoplasm / Q000494:pharmacology; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology*; D015820:Cadherins / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000503:physiopathology; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015843:Serpins / Q000276:immunology / Q000502:physiology*; D002583:Uterine Cervical Neoplasms
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015820:Cadherins; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D019869:Phosphatidylinositol 3-Kinases