[Experimental study of artificial bone composite of bicoral, rhBMP-2 and PLA in repairing calvarial defects].
OBJECTIVE
To study the effects of artificial bone composite of bicoral, rhBMP-2 and PLA in repairing calvarial critical-size defects.


METHODS
Calvarial defects in 24 rabbits were surgically made and then half of the defects were repaired with the artificial composite bone. Another half of them were repaired with bicoral/PLA composite and served as controls. Four rabbits in each group were sacrificed at 4, 8, 12 weeks after operation, respectively. The treatment effects were evaluated with scanning electron microscopy and mechanical strength testing.


RESULTS
New bone was observed not only in the periphery, but also inside the artificial bone in both groups, but earlier and more new bone formation was observed in treatment group compared with control group. The mechanical strength test showed that the artificial bone in two groups, which had same mechanical strength before implantation, had significant different mechanical strength after operation. The strength of the artificial composite bone was higher than that of controls and was same with normal rabbit calvarial bone.


CONCLUSIONS
The artificial composite bone possess a highly repairing ability, and the healing in bone defects may be accomplished by both osteoinductive and osteoconductive mechanism. The material may be used as a good substitute for bone grafting.
Chen
Xinmei
X
Department of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Mao
Tianqiu
T
Dai
Yimin
Y
D000818:Animals; D037421:Anthozoa; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000627:therapeutic use*; D020007:Implants, Experimental; D019344:Lactic Acid / Q000627:therapeutic use; D011091:Polyesters; D011108:Polymers / Q000627:therapeutic use; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D012886:Skull / Q000293:injuries* / Q000601:surgery; D016212:Transforming Growth Factor beta
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D019344:Lactic Acid; C033616:poly(lactide)