title: In vitro comparison of Essiac and Flor-Essence on human tumor cell lines.
abstract: Essiac (ES) and Flor-Essence (FE) are two herbal teas widely taken by North American cancer patients during chemo- and radiation therapy. In vitro studies on the antiproliferative and differentiation inducing activities of these teas were performed. ES and FE showed negligible antiproliferative activity on Jurkat leukemia cells. Both herbal teas inhibited 50% (IC50) of MCF7 breast cancer cell growth at 1/10 dilution. The IC50 was about 1/40 and 1/10 dilution of FE and ES respectively for MDA-MB-468 human breast cancer cells. The IC50 for HL60 cells was at 1/10 dilution of FE and less than 1/10 dilution of ES. ES at 1/10 dilution induced expression of non-specific esterase in 16% of HL60 cells, compared to about 5% in FE treated cells and untreated controls. ES treatment of HL60 cells induced 47-67% nitroblue tetrazolium positive staining cells compared to 24.6+/-3.1% in cells treated with 1/10 dilution of FE. Flow cytometry analysis showed that both ES and FE treatment between 1/10 and 1/100 dilutions only slightly affected the cell cycle progression of MCF7, MDA-MB-468, Jurkat and HL60 cells. Our data show that both ES and FE herbal teas demonstrated antiproliferative and differentiation inducing properties in vitro only at high concentrations. Further research is needed to elucidate the in vivo activities.
authors.affiliation: Center for Complementary Medicine Research, BC Research Institute for Children's and Women's Health, Room L306, 4480 Oak Street, Vancouver, British Columbia V5Z 4H4, Canada. jtai@interchange.ubc.ca
authors.forename: Joseph
authors.initials: J
authors.lastname: Tai
authors.forename: Susan
authors.initials: S
authors.lastname: Cheung
authors.forename: Stephen
authors.initials: S
authors.lastname: Wong
authors.forename: Christopher
authors.initials: C
authors.lastname: Lowe
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001628:Beverages; D001943:Breast Neoplasms; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; C111756:Essiac; D010936:Plant Extracts; C488083:flor-essence