[Possibilities of investigation and clinical application of adult human stem cells].
Multipotent adult tissue stem cells have high plasticity and transdifferentiation ability. The stem cell therapy can be the solution of curing many severe diseases such as osteogenesis imperfecta, hepatic failure, heart muscle damage after myocardial infarction, I-type diabetes, variety of central nervous system disorders such as brain injury, stroke, Parkinson's disease and other neurodegenerative disorders. Isolation of certain types of stem cells is solved nowadays, but low frequency of these cells and lack of special identification markers make their isolation and search more difficult. The more and more developed in vitro cell culture technologies, the widespread macromolecule amplification and examination techniques of molecular biology and the gene technology tools for genetic modification of stem (and other) cells contribute to research and therapeutic applications of stem cells. Transfer of new genetic material to stem cells and expression of the gene product in daughter cells--because missing or damaged genes can be replaced--is an exiting approach of the treatment of congenital (enzyme deficiencies) and acquired human diseases (cancers).
Galamb
Orsolya
O
Semmelweis Egyetem, Altalános Orvostudományi Kar, II. sz. Belgyógyászati Klinika MTA-Semmelweis Egyetem Gasztroenterológiai és Endokrinológiai Kutató Csoport, Budapest. orsg@freemail.hu
Molnár
Béla
B
Sipos
Ferenc
F
Tulassay
Zsolt
Z
D000328:Adult; D005784:Gene Amplification; D005822:Genetic Vectors; D015894:Genome, Human; D006412:Hematopoietic Stem Cells; D006801:Humans; D053685:Laser Therapy; D059630:Mesenchymal Stem Cells; D042282:Microdissection / Q000295:instrumentation; D020411:Oligonucleotide Array Sequence Analysis; D033581:Stem Cell Transplantation; D013234:Stem Cells