title: Heterologous expression of Zygosaccharomyces rouxii glycerol 3-phosphate dehydrogenase gene (ZrGPD1) and glycerol dehydrogenase gene (ZrGCY1) in Saccharomyces cerevisiae.
abstract: We examined the effects of heterologous expression of the open reading frames (ORF) of two genes on salt tolerance and glycerol production in a Saccharomyces cerevisiae strain deficient in glycerol synthesis (gpd1Deltagpd2Delta). When the ORF of the Zygosaccharomyces rouxii glycerol 3-phosphate dehydrogenase gene (ZrGPD1) was expressed under the control of the GAL10 promoter, salt tolerance and glycerol production increased; when the ORF of the glycerol dehydrogenase gene (ZrGCY1) was expressed under the control of the GAL1 promoter, no such changes were observed. Zrgcy1p had a weak effect on glycerol production. These results suggest that Zrgpd1p is the primary enzyme involved in Z. rouxii glycerol production, following a mechanism similar to that of S. cerevisiae (Gpd1p). When the ORFs of the S. cerevisiae glycerol 3-phosphatase gene (GPP2) and ZrGPD1 were simultaneously expressed, glycerol production increased, compared with that in yeast expressing only ZrGPD1.
authors.affiliation: Department of Biological Resources, Faculty of Agriculture, Ehime Univeristy, Matsuyama, Ehime790-8566, Japan. watanabe@agr.ehime-u.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Syoko
authors.initials: S
authors.lastname: Tsuchimoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D007700:Kinetics; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics*; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D012965:Sodium Chloride; D005993:Glycerolphosphate Dehydrogenase; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005990:Glycerol