Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
Wolffs
Petra
P
Applied Microbiology, Lund Institute of Technology, Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden.
Grage
Halfdan
H
Hagberg
Oskar
O
Rådström
Peter
P
D002021:Buffers; D004259:DNA-Directed DNA Polymerase / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011336:Probability; D015203:Reproducibility of Results
D002021:Buffers; D004259:DNA-Directed DNA Polymerase