Cell cycle regulation of NF-YC nuclear localization.
NF-Y is a trimeric activator with histone fold, HFM, subunits that binds to the CCAAT-box and is required for a majority of cell cycle promoters, often in conjunction with E2Fs. In vivo binding of NF-Y is dynamic during the cell cycle and correlates with gene activation. We performed immunofluorescence studies on endogenous, GFP- and Flag-tagged overexpressed NF-Y subunits. NF-YA, NF-YB are nuclear proteins. Unexpectedly, NF-YC localizes both in cytoplamatic and nuclear compartments and its nuclear localization is determined by the interaction with its heterodimerization partner NF-YB. Most importantly, compartmentalization is regulated during the cell cycle of serum restimulated NIH3T3 cells, accumulating in the nucleus at the onset of S phase. These data point to the control of HFM heterodimerization as an important layer of NF-Y regulation during cell cycle progression.
Frontini
Mattia
M
Dipartimento di Biologia Animale; Universita di Modena e Reggio Emilia, Modena, Italy.
Imbriano
Carol
C
Manni
Isabella
I
Mantovani
Roberto
R
D000818:Animals; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002451:Cell Compartmentation / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins; D006657:Histones / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D032446:Myoblasts / Q000378:metabolism; D041681:NIH 3T3 Cells; D021122:Protein Subunits / Q000378:metabolism
D023081:CCAAT-Binding Factor; D022762:CCAAT-Enhancer-Binding Proteins; D006657:Histones; D008164:Luminescent Proteins; D021122:Protein Subunits; D049452:Green Fluorescent Proteins