title: SS33410, an inhibitor of V-ATPase, blocks intracellular protein transport of the VSV-G protein in the Golgi compartment.
abstract: An earlier report suggested that SS33410, structurally related to folimycin and bafilomycin A(1), blocked secretion of the glycoprotein (G protein) of vesicular stomatitis virus (VSV) into the medium and, instead, G protein was accumulated intracellulary. To identify the inhibition site of SS33410 in intracellular protein transport, I have analyzed the oligosaccharide chain structure of the intracellularly accumulated G protein. In SS33410-treated VSV-infected cells, G protein oligosaccharide was suggested to have a composition of GlcNAc-Man(5)-GlcNAc(2) as analyzed by Bio-Gel P-4 column chromatography following digestion with alpha-mannosidase, beta-N-acetylhexosaminidase, and then with alpha-mannosidase. SS33410 specifically inhibited vacuolar-type ATPase (V-ATPase). These studies thus suggest that SS33410 blocks the intracellular protein transport before the step of trimming by mannosidase II, which is confined to the medial Golgi compartment.
authors.affiliation: Department of Microbiology, College of Medicine, Inje University, Busan, Korea. daehyun@ijnc.inje.ac.kr
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Seog
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D006056:Golgi Apparatus / Q000201:enzymology*; D008361:Mannosidases / Q000378:metabolism; D025942:Oligosaccharides, Branched-Chain / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D013302:Streptomyces; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D043323:alpha-Mannosidase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D025942:Oligosaccharides, Branched-Chain; D014759:Viral Envelope Proteins; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D043323:alpha-Mannosidase; D025262:Vacuolar Proton-Translocating ATPases