title: Expression and characterization of recombinant NH2-terminal cell binding fragment of vitronectin in E. coli.
abstract: A recombinant peptide fragment of vitronectin (rVN143), that includes the Arg-Gly-Asp (RGD) cell recognition site, was expressed in Escherichia coli using a prokaryotic expression system. The addition of recombinant rVN143 peptide enhances cell adhesion and proliferation similar (approximately 70%) to those of native VN.
authors.affiliation: Intellectual Biointerface Engineering Center, Seoul National University, College of Dentistry, Seoul 110-768, Korea.
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak
authors.forename: Sun-Chul
authors.initials: SC
authors.lastname: Kim
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Hwang
authors.forename: Jai-Bong
authors.initials: JB
authors.lastname: Lee
authors.forename: Ik-Tae
authors.initials: IT
authors.lastname: Jang
authors.forename: Chong-Pyoung
authors.initials: CP
authors.lastname: Chung
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
mesh_terms: D001665:Binding Sites; D040761:Biomimetic Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D013329:Structure-Activity Relationship; D019096:Vitronectin / Q000096:biosynthesis* / Q000145:classification / Q000494:pharmacology*
chemical_list: D010446:Peptide Fragments; D019096:Vitronectin