Serum from dogs Infected with Babesia gibsoni inhibits maturation of reticulocytes and erythrocyte 5'-nucleotidase activity in vitro.
Erythrocyte 5'-nucleotidase is thought to be involved in the maturation of erythrocytes. In the present study, in vitro incubation of canine erythrocytes demonstrated that significant inhibition of 5'-nucleotidase activity occurred in the presence of serum from dogs infected with Babesia gibsoni, when the enzyme was assayed with cytidine 5'-monophosphate (5'-CMP) and inosine 5'-monophosphate (5'-IMP) as substrates. The multiplication of B. gibsoni in in vitro culture also resulted in a significant decrease in the enzyme activity of erythrocytes in the culture. Furthermore, the infected serum and 5'-CMP retarded the maturation of canine reticulocytes in vitro. These results suggested that nucleotides such as 5'-CMP and 5'-IMP might accumulate in young erythrocytes and/or serum in dogs infected with B. gibsoni as a result of decreased activity of erythrocyte 5'-nucleotidase, resulting in the delayed maturation of reticulocytes.
Hossain
Mohammad Alamgir
MA
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Yamato
Osamu
O
Yamasaki
Masahiro
M
Jeong
Ja-Ryong
JR
Chang
Hye-Sook
HS
Maede
Yoshimitsu
Y
D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors / Q000097:blood*; D000818:Animals; D001403:Babesia / Q000472:pathogenicity*; D001404:Babesiosis / Q000097:blood / Q000662:veterinary*; D016922:Cellular Senescence / Q000502:physiology; D004283:Dog Diseases / Q000097:blood / Q000469:parasitology*; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology / Q000469:parasitology*; D012156:Reticulocytes / Q000469:parasitology*
D015720:5'-Nucleotidase