title: In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site.
abstract: Fat grafting is an unpredictable procedure that continues to challenge the field of plastic surgery due to irregular resorption. Applications for this procedure are broad in both reconstructive and cosmetic plastic surgery. Fat grafts are carefully obtained and manipulated to obtain better graft takes and results, yet there is no universal agreement on what constitutes an ideal methodology. The present study examines adipocyte viability from four commonly used donor sites in five subjects. No statistical differences in adipocyte viability were demonstrated among abdominal fat, thigh fat, flank fat, or knee fat donor sites that were immediately removed and untreated (p < 0.225). In addition, no differences were observed in representative tissue samples that were removed and centrifuged (thigh, p = 0.508; knee, p = 0.302; flank, p = 0.088; abdomen, p = 0.533). On the basis of these quantitative data, neither harvest location nor centrifugation demonstrated any advantage in terms of lipocyte viability. Fat tissue transfers from these common sites may be considered equal, and centrifugation does not appear to enhance immediate fat tissue viability before implantation.
authors.affiliation: Department of Plastic Surgery, University of Texas Southwestern Medical Center, Dallas 75390-9132, USA. rod.rohrich@utsouthwestern.edu
authors.forename: Rod J
authors.initials: RJ
authors.lastname: Rohrich
authors.forename: Evan S
authors.initials: ES
authors.lastname: Sorokin
authors.forename: Spencer A
authors.initials: SA
authors.lastname: Brown
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D002498:Centrifugation; D005562:Formazans; D006085:Graft Survival; D006801:Humans; D007202:Indicators and Reagents; D015187:Lipectomy; D020858:Tissue and Organ Harvesting
chemical_list: D005562:Formazans; D007202:Indicators and Reagents