Extracellular and intracellular volume variations during postural change measured by segmental and wrist-ankle bioimpedance spectroscopy.
Extracellular (ECW) and intracellular (ICW) volumes were measured using both segmental and wrist-ankle (W-A) bioimpedance spectroscopy (5-1000 kHz) in 15 healthy subjects (7 men, 8 women). In the 1st protocol, the subject, after sitting for 30 min, laid supine for at least 30 min. In the second protocol, the subject, who had been supine for 1 hr, sat up in bed for 10 min and returned to supine position for another hour. Segmental ECW and ICW resistances of legs, arms and trunk were measured by placing four voltage electrodes on wrist, shoulder, top of thigh and ankle and using Hanai's conductivity theory. W-A resistances were found to be very close to the sum of segmental resistances. When switching from sitting to supine (protocol 1), the mean ECW leg resistance increased by 18.2%, that of arm and W-A by 12.4%. Trunk resistance also increased but not significantly by 4.8%. Corresponding increases in ICW resistance were smaller for legs (3.7%) and arm (-0.7%) but larger for the trunk (21.4%). Total body ECW volumes from segmental measurements were in good agreement with W-A and Watson anthropomorphic correlation. The decrease in total ECW volume (when supine) calculated from segmental resistances was at 0.79 l less than the W-A one (1.12 l). Total ICW volume reductions were 3.4% (segmental) and 3.8% (W-A). Tests of protocol 2 confirmed that resistance and fluid volume values were not affected by a temporary position change.
Fenech
Marianne
M
Department of Biological Engineering, Technological University of Compiegne, 60205 Compiègne Cedex, France.
Jaffrin
Michel Y
MY
D000328:Adult; D000842:Ankle; D000886:Anthropometry / Q000379:methods; D001132:Arm / Q000502:physiology; D001825:Body Fluid Compartments / Q000502:physiology; D001834:Body Water / Q000502:physiology*; D017097:Electric Impedance; D045604:Extracellular Fluid / Q000502:physiology*; D005110:Extracellular Space / Q000502:physiology; D005260:Female; D018495:Fluid Shifts / Q000502:physiology*; D006801:Humans; D007424:Intracellular Fluid / Q000502:physiology*; D042541:Intracellular Space / Q000502:physiology; D007866:Leg / Q000502:physiology; D008297:Male; D010992:Plethysmography, Impedance / Q000379:methods*; D011187:Posture / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*; D013909:Thorax / Q000502:physiology; D014953:Wrist