title: Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.
abstract: The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.
authors.affiliation: Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Morishima
authors.forename: Minjun
authors.initials: M
authors.lastname: Chung
authors.forename: Ka Wing
authors.initials: KW
authors.lastname: Ng
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Brambilla
authors.forename: David R
authors.initials: DR
authors.lastname: Gretch
mesh_terms: D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic