title: Assays and affinity purification of biotinylated and nonbiotinylated forms of double-tagged core RNA polymerase II from Saccharomyces cerevisiae.
authors.affiliation: NCI Center for Cancer Research, National Cancer Institute, Frederick Cancer Research and Development Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702, USA.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Kireeva
authors.forename: Lucyna
authors.initials: L
authors.lastname: Lubkowska
authors.forename: Natalia
authors.initials: N
authors.lastname: Komissarova
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kashlev
mesh_terms: D001671:Biochemistry / Q000379:methods*; D019921:Biotinylation; D019731:Carbon-Nitrogen Ligases / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D011485:Protein Binding; D012319:RNA Polymerase II / Q000737:chemistry*; D012097:Repressor Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012685:Sepharose / Q000737:chemistry; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D006639:Histidine; D009532:Nickel; D004247:DNA; D012685:Sepharose; D005982:Glutathione Transferase; D012319:RNA Polymerase II; D019731:Carbon-Nitrogen Ligases; C058125:birA protein, E coli