Enzyme activity--it's all about image.
Unraveling the functional roles of proteins is a major challenge facing the postgenome researcher. Advances towards this goal have been made through the development of both chemical and biochemical tools for monitoring protein activity. Recently, a myriad of fluorescence-based imaging tools have emerged for in vitro, in vivo and whole animal applications. These tools have provided methods to monitor the spatial and temporal distribution of proteins and bioorganic molecules dynamically. Here, recent advances in chemical and biochemical techniques that allow the detection of enzymatic activity within intact cells and in vivo are reviewed. Such technologies have the potential to be integrated into drug-development programs to facilitate both the functional validation of pharmaceutical targets and the treatment of human disease.
Baruch
Amos
A
Department of Chemical Proteomics, Celera Genomics, 180 Kimball Way, South San Francisco, CA 94080, USA. baruchan@celera.com
Jeffery
Douglas A
DA
Bogyo
Matthew
M
D000818:Animals; D010450:Endopeptidases / Q000378:metabolism; D004798:Enzymes / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D010770:Phosphotransferases / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
D004798:Enzymes; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D010770:Phosphotransferases; D010450:Endopeptidases