Fecal immunoglobulin A antibodies in dogs infected or vaccinated with canine coronavirus.
Fecal secretory immunoglobulin A (IgA) antibodies in dogs infected or vaccinated with canine coronavirus (CCV) were evaluated by an enzyme-linked immunosorbent assay. The study was carried out with 32 fecal samples collected just before inoculation and at 28 days postinoculation. Five groups were studied: naturally infected dogs, experimentally infected dogs, dogs inoculated with a modified live (ML) CCV vaccine by the intramuscular route, dogs inoculated with an ML CCV vaccine by the oronasal route, and dogs given an inactivated CCV vaccine. Both the naturally and the experimentally infected dogs developed high levels of fecal IgAs. Interestingly, dogs inoculated with the ML CCV vaccine by the oronasal route developed levels of fecal IgA that were higher than those observed in the dogs inoculated with the same CCV vaccine by the intramuscular route or those observed in dogs inoculated with the inactivated vaccine. A relationship between the level of fecal IgAs to CCV and the degree of protection against CCV infection was observed.
Decaro
Nicola
N
Department of Animal Health and Well-Being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy. n.decaro@veterinaria.uniba.it
Pratelli
Annamaria
A
Tinelli
Antonella
A
Martella
Vito
V
Camero
Michele
M
Buonavoglia
Domenico
D
Tempesta
Maria
M
Caroli
Anna Maria
AM
Buonavoglia
Canio
C
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017939:Coronavirus, Canine / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology* / Q000517:prevention & control; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D007070:Immunoglobulin A / Q000032:analysis*; D014765:Viral Vaccines / Q000008:administration & dosage
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D014765:Viral Vaccines