Demineralization in enamel and hydroxyapatite aggregates at increasing ionic strengths.
Subsurface demineralization of dental enamel is a curious feature of both in vivo and in vitro lesion formation. Numerous explanations have been proposed to explain this. One general hypothesis is that subsurface demineralization in enamel and synthetic hydroxyapatite (HAP) aggregates may result from the phenomenon of coupled diffusion between the inward transport of acid and the outward transport of dissolution products. The aim of this study was to test the validity of this explanation. Inert electrolyte was added to demineralizing solutions in order to reduce electrostatic coupling between the diffusive flows that occur during lesion formation. Scanning microradiography (SMR) was used to examine surface layer formation, and to measure the rate of mineral loss at increasing ionic strengths. It was found that surface layer formation was significantly reduced as the concentration of inert electrolyte was increased. Further, the rate of mineral loss from the developing lesion increased as the concentration of inert electrolyte (and therefore the ionic strength) in the demineralizing solution increased. It is concluded that electrostatic coupling between counter diffusing acid and dissolution products during lesion formation can significantly influence the mineral concentration within the surface layer.
Anderson
P
P
Centre for Oral Growth and Development, Dental Biophysics Section, Medical Sciences Building, Queen Mary, University of London, Mile End Road, E1 4NS, London, UK. p.anderson@qmul.ac.uk
Bollet-Quivogne
F R G
FR
Dowker
S E P
SE
Elliott
J C
JC
D003743:Dental Enamel / Q000187:drug effects; D003747:Dental Enamel Solubility / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000737:chemistry*; D004572:Electrolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008852:Microradiography; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D017001:Tooth Demineralization / Q000378:metabolism*
D011189:Potassium Chloride; D017886:Durapatite