Green tea polyphenol (-)-epigallocatechin-3-gallate protects rat PC12 cells from apoptosis induced by serum withdrawal independent of P13-Akt pathway.
Our recent studies have demonstrated that green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) exerts neuroprotective/neurorescue effects against B-amyloid toxicity and protects neuronal cells from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridinium ion (MPP+) and 6-hydroxydopamine in vitro, or from N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- (MPTP-) induced nigral dopaminergic neuronal loss in mice. In the present study, we report that EGCG (0.1 and 1 microM) significantly protects rat pheochromocytoma PC12 cells from apoptosis induced by serum support withdrawal, suggesting that EGCG may play a role in the growth of PC12 cells, where it stimulates survival-promoting pathways.
Mandel
Silvia
S
Eve Topf and USA National Parkinson Foundation Centers of Excellence for Neurodegenerative Diseases Research, Technion-Faculty of Medicine, Haifa 31096, Israel.
Reznichenko
Lydia
L
Amit
Tamar
T
Youdim
Moussa B H
MB
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats
D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D002392:Catechin; C045651:epigallocatechin gallate; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases