title: Overexpression of leptin mRNA in mesenteric adipose tissue in inflammatory bowel diseases.
abstract: BACKGROUND
  Leptin, a protein with a cytokine-like structure, is produced predominantly by adipocytes. It appears to play a key role in immune responses by increasing the secretion of Th1 and pro-inflammatory cytokines. As fat-wrapping is a characteristic feature of Crohn's disease (CD), and as increased leptin levels have been reported in animal models of intestinal inflammation, this study investigated whether mesenteric adipose tissue could be a source of leptin in human inflammatory bowel disease (IBD).
  
  
  OBJECTIVE
  To quantify the expression of leptin mRNA in mesenteric adipose tissue of patients with CD or ulcerative colitis (UC).
  
  
  METHODS
  Specimens were obtained from mesenteric white adipose tissue close to healthy and inflammatory small intestine and/or colon in patients with CD or UC and, for controls, from apparently healthy mesentery of patients operated for carcinoma of the right colon. The expression of leptin mRNA was assessed by reverse transcription-competitive polymerase chain reaction.
  
  
  RESULTS
  Leptin mRNA levels were significantly higher in mesenteric adipose tissue of CD and UC patients than in controls (P<0.05). In CD and UC, concentrations were not significantly different in mesenteric fat specimens, whether contiguous to macroscopically normal or grossly abnormal intestine.
  
  
  CONCLUSIONS
  This study provides the first evidence of a novel abnormality of the mesentery of patients with IBD. Overexpression of leptin mRNA in mesenteric adipose tissue may contribute to (a) the inflammatory process, (b) enhancement of mesenteric TNF alpha expression in CD (as recently reported), and/or (c) the anorexia frequently reported during flares of IBD.
authors.affiliation: Pôle Digestif et CIC-INSERM, CHU Nantes et INRA, 44093 Nantes Cedex 1.
authors.forename: Maryse
authors.initials: M
authors.lastname: Barbier
authors.forename: Hubert
authors.initials: H
authors.lastname: Vidal
authors.forename: Pierre
authors.initials: P
authors.lastname: Desreumaux
authors.forename: Laurent
authors.initials: L
authors.lastname: Dubuquoy
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bourreille
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Colombel
authors.forename: Christine
authors.initials: C
authors.lastname: Cherbut
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Galmiche
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D020738:Leptin / Q000235:genetics*; D008297:Male; D008643:Mesentery; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*
chemical_list: D020738:Leptin; D012333:RNA, Messenger