Root border cells take up and release glucose-C.
OBJECTIVE
Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.


METHODS
Border cells were removed from Zea mays L. root tips, and immersed in (14)C-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.


RESULTS
Glucose uptake by the border cells increased with increasing glucose concentration from 0.2 to 20 mm. At 0.2 mm glucose, uptake was mainly active, as evidenced by the approx. 60 % inhibition with phlorizin. At 2 and 20 mm glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mm glucose. There was no clear efflux/time pattern for heat-killed border cells.


CONCLUSIONS
Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions.
Stubbs
V E C
VE
Plant-Soil Interface Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Standing
D
D
Knox
O G G
OG
Killham
K
K
Bengough
A G
AG
Griffiths
B
B
D001692:Biological Transport; D005947:Glucose / Q000378:metabolism*; D010695:Phlorhizin / Q000494:pharmacology; D018517:Plant Roots / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D003313:Zea mays / Q000166:cytology / Q000502:physiology
D010695:Phlorhizin; D005947:Glucose