[Preparation of the microarray for detecting hepatitis D virus].
OBJECTIVE
To prepare the microarray for detection of hepatitis D virus (HDV).


METHODS
Several pairs of specific PCR primers were designed according to the conserved region of HDV genome. The DNA microarray were prepared by blotting the PCR products onto the surface of glass slides with the use of robotics, and restriction display PCR (RD-PCR) was employed to label the samples.


RESULTS
Sequences analysis showed that the products of PCR amplification were the specific gene fragments of HDV. Hybridization signals on the gene chip demonstrated good specificity and sensitivity of the microarray for HDV detection.


CONCLUSIONS
Microarray-based clinical HDV detection can be sensitive and effective.
Sun
Zhao-hui
ZH
Institute of Molecular Oncology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
Zheng
Wen-ling
WL
Zhang
Bao
B
Shi
Rong
R
Ma
Wen-li
WL
D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood
D012367:RNA, Viral