Localization of dopamine D1-receptor to A-type horizontal cells in the rabbit retina by single cell RT-PCR.
Dopamine is an important neurotransmitter regulating light-dark adaptation in the retina. The effect of dopamine is widespread and dependent on the localization of its receptors. We performed single cell RT-PCR on A-type horizontal cells visually selected from dissociated rabbit retinal neurons. Dopamine D1-receptor mRNA was positively identified. Subsequent sequencing of the fragment showed 82% homology with rat D1 receptor and 87% homology with human D1 receptor. This study supported previous observations that dopamine regulated A-type horizontal cell coupling via D1 receptors at the level of transcription.
Li
Hui
H
Institute of Neuroscience and Shanghai Research Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Gaughwin
Phil
P
Li
Ning
N
He
Shigang
S
D000221:Adaptation, Ocular / Q000235:genetics; D000818:Animals; D002469:Cell Separation; D003623:Dark Adaptation / Q000235:genetics; D004298:Dopamine / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism*; D011817:Rabbits; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D009435:Synaptic Transmission / Q000235:genetics
D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D004298:Dopamine