title: Drosophila doubletime mutations which either shorten or lengthen the period of circadian rhythms decrease the protein kinase activity of casein kinase I.
abstract: In both mammals and fruit flies, casein kinase I has been shown to regulate the circadian phosphorylation of the period protein (PER). This phosphorylation regulates the timing of PER's nuclear accumulation and decline, and it is necessary for the generation of circadian rhythms. In Drosophila melanogaster, mutations affecting a casein kinase I (CKI) ortholog called doubletime (dbt) can produce short or long periods. The effects of both a short-period (dbt(S)) and long-period (dbt(L)) mutation on DBT expression and biochemistry were analyzed. Immunoblot analysis of DBT in fly heads showed that both the dbt(S) and dbt(L) mutants express DBT at constant levels throughout the day. Glutathione S-transferase pull-down assays and coimmunoprecipitation of DBT and PER showed that wild-type DBT, DBT(S), and DBT(L) proteins can bind to PER equivalently and that these interactions are mediated by the evolutionarily conserved N-terminal part of DBT. However, both the dbt(S) and dbt(L) mutations reduced the CKI-7-sensitive kinase activity of an orthologous Xenopus laevis CKIdelta expressed in Escherichia coli. Moreover, expression of DBT in Drosophila S2 cells produced a CKI-7-sensitive kinase activity which was reduced by both the dbt(S) and dbt(L) mutations. Thus, lowered enzyme activity is associated with both short-period and long-period phenotypes.
authors.affiliation: School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA.
authors.forename: Fabian
authors.initials: F
authors.lastname: Preuss
authors.forename: Jin-Yuan
authors.initials: JY
authors.lastname: Fan
authors.forename: Madhavi
authors.initials: M
authors.lastname: Kalive
authors.forename: Shu
authors.initials: S
authors.lastname: Bao
authors.forename: Eric
authors.initials: E
authors.lastname: Schuenemann
authors.forename: Edward S
authors.initials: ES
authors.lastname: Bjes
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Price
mesh_terms: D000818:Animals; D001483:Base Sequence; D047388:Casein Kinases; D002460:Cell Line; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D017344:Genes, Insect; D066298:In Vitro Techniques; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D056950:Period Circadian Proteins; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D011494:Protein Kinases; D047388:Casein Kinases