title: [Arrangement of the sense and terminating codons of the template in the A-segment of human ribosomes from photocrosslinking data withe oligonucleotide derivatives].
abstract: Oligoribonucleotide derivatives containing Phe codon UUC along with a 3'-flanking sense codon or stop codon carrying a perfluoroarylazido group at G or U were used to study the position of each nucleotide of the latter codon relative to the 18S rRNA in the A site of the 80S ribosome. To place the modified sense or stop codon in the A site, UCC-recognizing tRNA(Phe) was bound in the P site. Regardless of the position in the sense or stop codon, the modified nucleotide crosslinked with invariant dinucleotide A1823/A1824 or nucleotide A1825 in helix 44 close to the 3' end of the 18S rRNA. Located in the second or third position of either codon, the modified G bound with invariant nucleotide G626, which is in the evolutionarily conserved 530 stem-loop segment. The results were collated with the X-ray structure of the bacterial ribosome, and the template codon was assumed to be similarly arranged relative to the small-subunit rRNA in various organisms.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Stiazhkina
authors.forename: M V
authors.initials: MV
authors.lastname: Molotkov
authors.forename: N A
authors.initials: NA
authors.lastname: Demeshkina
authors.forename: K N
authors.initials: KN
authors.lastname: Bulygin
authors.forename: D M
authors.initials: DM
authors.lastname: Graĭfer
authors.forename: M I
authors.initials: MI
authors.lastname: Meshchaninova
authors.forename: M N
authors.initials: MN
authors.lastname: Repkova
authors.forename: A G
authors.initials: AG
authors.lastname: Ven'iaminova
authors.forename: G G
authors.initials: GG
authors.lastname: Karpova
mesh_terms: D001483:Base Sequence; D018388:Codon, Terminator; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D010777:Photochemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D018388:Codon, Terminator; D009843:Oligoribonucleotides; D012336:RNA, Ribosomal, 16S