[Expression of human telomerase reverse transcriptase and bcl-2 in ameloblastoma].
OBJECTIVE
To study the expression of telomerase reverse transcriptase (hTERT) and bcl-2 in ameloblastoma (AB), METHODS: hTERT mRNA in 54 cases of AB (primary AB 31 cases, recurrent AB 17 cases, malignant AB 4 cases) and 7 cases of oral normal mucosa was detected by in situ hybridization, and bcl-2 by S-P method.


RESULTS
The expression of hTERT mRNA was negative or weak in normal oral mucosa (14.3%), moderate or strong in AB (94.4%). There was a significant difference in these two groups (P < 0.001). The difference between the expressions of hTERT in primary, recurrent and malignant AB was significant (P < 0.05). The positive ratio of bcl-2 in AB and normal oral mucosa was respectively 88.0%, 44.4%. There was a significant statistical difference in these two groups (P < 0.001). hTERT mRNA was stronger in recurred or malignantly transformed AB (P < 0.05).


CONCLUSIONS
The expression of hTERT and bcl-2 is stronger in recurred or malignantly transformed AB, and it could be used as an indicator of AB prognosis. Telomerase activity and bcl-2 expression play an important role in genesis and development of AB.
Wang
Jie
J
Department of Pathology, College of Stomatology, China Medical University, Shenyang 110002, China.
Zhong
Ming
M
Wang
Zhaoyuan
Z
D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000201:enzymology* / Q000378:metabolism; D014408:Biomarkers, Tumor; D002648:Child; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D007573:Jaw Neoplasms / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000201:enzymology / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D019098:Telomerase