PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers.
In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5'-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.
Kotłowski
Roman
R
Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
Shamputa
Isdore Chola
IC
El Aila
Nabil Abdullah
NA
Sajduda
Anna
A
Rigouts
Leen
L
van Deun
Armand
A
Portaels
Françoise
F
D016172:DNA Fingerprinting; D017931:DNA Primers; D004251:DNA Transposable Elements; D005838:Genotype; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004251:DNA Transposable Elements