title: Immunohistochemical evaluation of mononuclear infiltrates in canine lupoid onychodystrophy.
abstract: Claw biopsy samples of 11 dogs with lupoid onychodystrophy were evaluated. They were stained with hematoxylin and eosin and with antibodies against CD 3 as a T-cell marker, BLA 36 and HM 57 (CD 79alpha) as B-cell markers, and lysozyme, Mac 387, and major histocompatibility complex (MHC) class II as a marker for histiocytes using an immunoperoxidase and avidin-biotin technique. Inflammatory cells were counted in five high-power fields. The inflammatory infiltrate comprised predominantly B cells and T cells. Macrophages were typically only present in small numbers. CD 3, BLA 36, lysozyme, and MHC class II preserved significant antigenicity during formalin fixation and short decalcification for 24-48 hours, whereas CD 79alpha and particularly Mac 387 seemed to be more susceptible to denaturation by the decalcification process.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA. ralf.mueller@med.vetmed.uni-muenchen.de
authors.forename: R S
authors.initials: RS
authors.lastname: Mueller
authors.forename: K
authors.initials: K
authors.lastname: West
authors.forename: S V
authors.initials: SV
authors.lastname: Bettenay
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002452:Cell Count; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005534:Foot Diseases / Q000276:immunology / Q000662:veterinary*; D006644:Histiocytes / Q000276:immunology; D006724:Hoof and Claw / Q000276:immunology* / Q000473:pathology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000662:veterinary*
chemical_list: D000906:Antibodies