Correlated expression of inducible nitric oxide synthase and P53, Bax in benign and malignant diseased gallbladder.
Our goal has been to investigate the expression and correlated significance of inducible nitric oxide synthase (iNOS) and P53, Bax in benign and malignant gallbladder diseases. We detected the expression of iNOS, P53 and Bax in the gallbladder wall by SP immunohistochemistry in 16 cases of chronic cholecystitis, 11 cases of chronic cholecystitis with adenomyoma and 24 cases of gallbladder adenocarcinoma. The percentage of positively marked tumor cells was counted under microscope and the intensity of immunoreactivity was graded. SPSS10.0 statistical software was applied for statistical analysis. In this study, we found that: (1) Both benign and malignant diseased gallbladder wall expressed iNOS and Bax. Compared to benign diseased gallbladders, their expression in adenocarcinoma was decreased (p < 0.05), P53 was expressed strongly only in nuclei of adenocarcinoma cells of some cases. (2) In benign and malignant diseased gallbladders, iNOS expression was related positively to Bax (p < 0.01), the expression of P53 and Bax had a negative relationship (p < 0.01). The results suggested that both chronic cholecystitis and chronic cholecystitis with adenomyoma carry the risk of becoming malignant, especially the latter. NO is an important mediated molecule in cancer, there are intimate relationships between gallbladder cancer and apoptosis.
Zhang
Ming
M
Department of Anatomy, Medical College of Zhejiang University, 353 Yan An Road, Hangzhou, Zhejiang 310006, China. jbxue001@zju.edu.cn
Pan
Jian-Wei
JW
Ren
Tian-Rong
TR
Zhu
You-Fa
YF
Han
Yong-Jian
YJ
Kühnel
Wolfgang
W
D000208:Acute Disease; D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis; D002763:Cholecystectomy; D002764:Cholecystitis / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002908:Chronic Disease; D005706:Gallbladder Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009092:Mucous Membrane / Q000201:enzymology / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II