[Camouflage of HLA-I antigen in lymphocyte surface].
The objective of this study was to investigate the method and effect of blocking the specific reaction between lymphocyte HLA-I antigen and its antibody. The lymphocytes were disposed with 12 mmol/L methoxypolyethelene glycol benzotriazol carbonate (mPEG-BTC) in concentration gradient in PBS (pH 7.4) at 22 degrees C. The effect of the modified lymphocytes was detected by microlymphocytotoxicity assay. The results showed that lymphocytes modified by mPEG-BTC did not react with related HLA-I antibodies in microcytotoxicity test. It is suggested that the specific reaction between HLA-I antigen of lymphocyte and HLA-I antibodies can be completely camouflaged by mPEG-BTC in PBS (pH 7.4) under 22 degrees C room temperature.
Zhang
Yin-Ze
YZ
Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. zyz200157@sohu.com
Li
Wei
W
Zhou
Hua-You
HY
Xia
Rong
R
Lan
Jong-Cai
JC
Zhang
Yang-Pei
YP
Zhang
Zhi-Xin
ZX
D000937:Antigen-Antibody Reactions; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D015395:Histocompatibility Antigens Class I; D014230:Triazoles; D011092:Polyethylene Glycols; C012771:benzotriazole; C028210:monomethoxypolyethylene glycol