title: Fourier transform infrared spectroscopy application to vascular biology: comparative analysis of human internal mammary artery and saphenous vein wall.
abstract: Saphenous vein (SV) and internal mammary artery (IMA) are used for aorto-coronary bypass grafting. IMA is considered to be the graft of choice for coronary revascularization having a long-term patency compared to SV. The aim of this study is to investigate the structure of vascular wall using a new technical approach. We analysed the chemical composition of vessel wall layers (total lipid, lipid ester and protein) of 25 vascular segments (19 SV and 6 IMA) using Fourier transform infrared spectroscopy (FTIR). FTIR analysis showed that in intima layer lipid ester and protein concentration (expressed as arbitrary units) was significantly higher in SV (lipid ester = 0.020 +/- 0.002; protein = 0.449 +/- 0.022) than in IMA (lipid ester = 0.014 +/- 0.002; protein = 0.342 +/- 0.032). Moreover, the percentage of lipid ester on total lipid was significantly higher in SV (intima = 54.7 +/- 2.9%; media = 78.4 +/- 4.9%; adventitia = 83.9 +/- 8.3%) wall layers compared to IMA ones (intima = 37.3 +/- 4.9%; media = 45.4 +/- 3.8; adventitia = 57.1 +/- 4.8). These data suggest that a different chemical composition of wall layers could also be responsible for the morphological modifications observed in SV after grafting.
authors.affiliation: Human Anatomy Laboratory, Medical Sciences Department, University of Eastern Piedmont A. Avogadro, Novara, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Renò
authors.forename: M
authors.initials: M
authors.lastname: Sabbatini
authors.forename: M
authors.initials: M
authors.lastname: Bosetti
authors.forename: G
authors.initials: G
authors.lastname: Laroche
authors.forename: D
authors.initials: D
authors.lastname: Mantovani
authors.forename: M
authors.initials: M
authors.lastname: Cannas
mesh_terms: D000368:Aged; D001026:Coronary Artery Bypass / Q000379:methods; D003324:Coronary Artery Disease / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D023903:Coronary Restenosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis; D008297:Male; D008323:Mammary Arteries / Q000737:chemistry* / Q000166:cytology / Q000637:transplantation; D008875:Middle Aged; D011506:Proteins / Q000032:analysis; D012501:Saphenous Vein / Q000737:chemistry* / Q000166:cytology / Q000637:transplantation; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D011506:Proteins