title: [Identification on an antagonistic rhizobacterium X3 from rhizosphere of cucumber].
abstract: Classical physiological and biochemical methods, Biolog, and 16S rDNA analysis were used to identify the strain X3 which could control cucumber seedling damping-off. Physiological and biochemical identification showed that it belonged to Pseudomanas aeruginosa, but Biolog identification showed it was P. spinosa. 16S rDNA determination and analysis was used for further identification, which showed that the 16S rDNA sequence of X3 shared 93.7% homologies with published sequence of P. aeruginosa from GenBank, and both sequences constituted a branch in Phylogenetic tree. Based on these results, it is considered that the strain X3 belongs to one strain of P. aeruginosa.
authors.affiliation: Zhejiang University, Hangzhou 310029, China. mailahshen@sohu.com
authors.forename: Aihua
authors.initials: A
authors.lastname: Shen
authors.forename: Bingxin
authors.initials: B
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Lirong
authors.initials: L
authors.lastname: Shen
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D018553:Cucumis sativus / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000737:chemistry; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S