A perfused rat brain model maintaining the connection between the central and peripheral nervous systems.
We have developed a new perfused brain model in rats. In this model, the cerebral circulation is separated from the systemic circulation, while the connections between the central and peripheral nervous systems are preserved. After bilateral common carotid, external carotid and vertebral artery ligation, bilateral common carotid arteries were cannulated to infuse rinsed human type O red blood cells mixed with modified Ringer's solution. To drain cerebral venous blood, external jugular veins were cannulated. Normal electrocortical activities were observed on electroencephalograms (EEGs) for more than 1h after the beginning of the perfusion. Somatosensory evoked potentials (SEPs) were also recorded. Direct infusion of pentylenetetrazol (PTZ) into the brain induced epileptic discharges on the EEGs and active dilation of cerebral arterioles, which was accompanied by an increase in systemic blood pressure (BP). The present model, in which we can change cerebral blood flow (CBF) and/or cerebral metabolism without directly affecting the systemic circulation, will provide a new approach to brain research.
Kobayashi
Norio
N
Biophysics Group, Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan.
Hoshi
Yoko
Y
Tamura
Mamoru
M
D000818:Animals; D001187:Artificial Organs; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology*; D017536:Carotid Artery, Common / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery; D002490:Central Nervous System / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D004569:Electroencephalography; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D008297:Male; D023421:Models, Animal; D010433:Pentylenetetrazole / Q000494:pharmacology; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D017933:Peripheral Nervous System / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
D010433:Pentylenetetrazole