Resveratrol inhibits cell proliferation and induces apoptosis of human breast carcinoma MCF-7 cells.
Resveratrol, which is found in grapes and wine, has been reported to have a variety of important pharmacological effects including anti-inflammatory, anti-platelet, and anti-carcinogenetic properties. In this study, using the human breast cancer cell line MCF-7, we have analyzed a possible mechanism by which resveratrol could interfere with cell cycle control and induce cell death. Resveratrol treatment of MCF-7 cells resulted in a dose-dependent inhibition of the cell growth and the cells accumulated at the S phase transition of the cell cycle at low concentrations, but high concentrations do not induce S phase accumulation. The anti-proliferative effects of resveratrol were associated with a marked inhibition of cyclin D and cyclin-dependent kinase (Cdk) 4 proteins, and induction of p53 and Cdk inhibitor p21WAF1/CIP. Growth suppression by resveratrol was also due to apoptosis, as seen by the appearance of a sub-G1 fraction and chromatin condensation. In addition, the apoptotic process involves activation of caspase-9, a decrease of Bcl-2 as well as Bcl-XL levels, and an increase of Bax levels.
Kim
Young-Ae
YA
Department of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine, San 45-1, Yangjung-dong, Pusanjin-gu, Busan 614-052, Korea.
Choi
Byung Tae
BT
Lee
Yong Tae
YT
Park
Dong Il
DI
Rhee
Sook-Hee
SH
Park
Kun-Young
KY
Choi
Yung Hyun
YH
D016588:Anticarcinogenic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D008517:Phytotherapy; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000633:toxicity*; D027843:Vitis; D014920:Wine
D016588:Anticarcinogenic Agents; D017931:DNA Primers; D009363:Neoplasm Proteins; D013267:Stilbenes; D000077185:Resveratrol