Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai.
Microsatellite markers have been developed for a variety of abalones, and locus-specific homozygote excesses at population level have been recorded for microsatellite loci. To ascertain whether null alleles exist at microsatellite loci in the Pacific abalone, we studied the mode of inheritance of 7 microsatellite loci in 4 families with a reciprocal cross of 2 females x 2 males. All loci segregated codominantly, but only 3 loci ( Hdh1321, Hdh78, and Hdd108C) conformed to Mendelian segregation and can be used for parental analysis and population genetic studies. When null alleles were considered, 2 loci (Hdh1761 and Hdh1457) confirmed Mendelian expectations in all families, while the remaining 2 loci (Hdd114B and Hdd229) showed deviation from Mendelian segregation in at least one family even though null alleles were considered. These results indicated the need to test the inheritance pattern for microsatellite markers in abalones before using them for population genetic of parentage analysis.
Li
Qi
Q
Fisheries College Ocean University of China, Qingdao 266003, China.
Park
Choulji
C
Kobayashi
Toshimasa
T
Kijima
Akihiro
A
D000818:Animals; D003433:Crosses, Genetic; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005828:Genetics, Population; D040582:Inheritance Patterns / Q000235:genetics*; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics*; D008974:Mollusca / Q000235:genetics*
D017931:DNA Primers