A semi-micromethod for determination of oxalate in human plasma.
An enzymatic semi-micromethod for oxalate determination in human plasma was elaborated. The principle of the method depends on the oxalate isolation from deproteinized plasma, following determination by the calorimetric oxalate oxidase-peroxidase-indamine system. This method protects against internal oxalate losses and excludes an interference of contaminations. Results, obtained by this method, were reliable and ideally suited for use as real normal values (less than or equal to 6 microM) of oxalate content in the plasma of healthy individuals. The elaborated method, which can assay plasma oxalate accurately in normal individuals as well as in hyperoxalemic conditions is superior to those previously used. The procedure of semi-micromethod does not require expensive equipments and apparatus: it is simple and easy to perform in every laboratory and takes little time.
Porowski
Tadeusz
T
Clinic of Children Diseases Medical Academy, 15-274 Bialystok, 17 Waszyngtona Str., Poland. tporowski@pb.bialystok.pl
Gałasiński
Władysław
W
D000465:Algorithms; D000818:Animals; D002138:Calibration; D003124:Colorimetry; D006801:Humans; D010070:Oxalates / Q000097:blood*; D010088:Oxidoreductases; D012016:Reference Values; D015203:Reproducibility of Results; D013552:Swine
D010070:Oxalates; D010088:Oxidoreductases; C020598:oxalate oxidase