Aminopeptidases in visceral organs during alterations in body fluid volume and osmolality.
Enzymatic cleavage of some peptides in the local environment could be included among the mechanisms related to the regulation of hydrosaline balance. In order to examine this hypothesis, we measured representative aminopeptidase activities in visceral organs of rats after applying certain hydrosaline challenges. Decreased levels (about 30%) of particulate puromycin-insensitive-neutral aminopeptidase in the renal medulla and of soluble acid aminopeptidase in the lung were observed under hyperosmolality and hypovolemia. Decreased levels (more than 45%) of particulate type-I-pyroglutamyl aminopeptidase in the heart were observed under altered volemia. These results indicate that aminopeptidases at these anatomical locations might be involved in the regulation of body fluid volume and osmolality.
Gasparello-Clemente
Elaine
E
Laboratory of Pharmacology, Instituto Butantan, Av. Vital Brazil 1500, 05503-900, São Paulo, SP, Brazil.
Casis
Luis
L
Varona
Adolfo
A
Gil
Javier
J
Irazusta
Jon
J
Silveira
Paulo Flávio
PF
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001769:Blood / Q000378:metabolism; D001826:Body Fluids / Q000502:physiology*; D001835:Body Weight; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D014781:Viscera / Q000201:enzymology*; D014882:Water-Electrolyte Balance / Q000502:physiology*
D000626:Aminopeptidases