Protein profile of symbiotic bacteria Mesorhizobium loti MAFF303099 in mid-growth phase.
Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications.
Kajiwara
Hideyuki
H
Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. kajiwara@affrc.go.jp
Kaneko
Takayo
T
Ishizaka
Masami
M
Tajima
Shigeyuki
S
Kouchi
Hiroshi
H
D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D000070116:Lotus / Q000382:microbiology; D020560:Proteobacteria / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis / Q000502:physiology
D020543:Proteome