Therapy with dendritic cells influences the spontaneous resorption rate in the CBA/J x DBA/2J mouse model.
OBJECTIVE
DBA/2J-mated CBA/J female mice are prone to a high incidence of fetal abortions. This fetal wastage can be dramatically reduced by immunizing the female mice with BALB/c, but not with DBA/2J spleen cells during early gestation. Nevertheless, the underlying mechanisms remain to be elucidated. Recently, dendritic cells (DC) have been described at the feto-maternal interface in the human uterus. In this work, we studied the effect of adoptive transfer of DC on the maintenance of pregnancy in the CBA/J x DBA/2J model.


METHODS
Bone marrow-derived DC were generated from virgin female CBA/J mice (6-8 weeks old). CBA/J females were inoculated with DC twice before mating. Four different experimental groups were included: (i) no treatment control, (ii) mice injected with culture medium [granulocyte-macrophage colony-stimulating factor (GM-CSF)], (iii) immunized with DC and (iv) immunized with paternal DBA/2J antigens lisate-pulsed DC, n = 5.


RESULTS
The control abortion rate was 23.8%, and with GM-CSF alone was 17.6%. Following inoculation of syngeneic DC abortion rates were reduced to 2.2%, but protection was short-lived. Abortion rates with DC pulsed with DBA/2J antigens was 5%. Serum of interleukin (IL)-6 levels were lower in the latter two groups up to the time of abortion. The kinetics of immunoglobulin G asymmetric antibodies synthesis was modified, but there was no correlation between asymmetric antibodies production and the lowering of abortions rates.


CONCLUSIONS
Syngeneic DC prevented abortions and this was linked to a decrease in IL-6 levels, but not with levels of asymmetric antibodies.
Blois
Sandra
S
IDEHU, Institute of Humoral Immunity Studies, National Council of Scientific and Technological Research (CONICET), University of Buenos Aires, Buenos Aires, Argentina. sblois@ffyb.uab.ar
Alba Soto
Catalina D
CD
Olmos
Sofia
S
Chuluyan
Eduardo
E
Gentile
Teresa
T
Arck
Petra C
PC
Margni
Ricardo A
RA
D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D002478:Cells, Cultured; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation*; D020964:Embryo Loss / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy*; D005260:Female; D005434:Flow Cytometry; D015850:Interleukin-6 / Q000097:blood; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010641:Phenotype; D011247:Pregnancy
D000906:Antibodies; D015850:Interleukin-6