Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs.
In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.
Brouta
Frédéric
F
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Descamps
Frédéric
F
Vermout
Sandy
S
Monod
Michel
M
Losson
Bertrand
B
Mignon
Bernard
B
D032761:Animal Experimentation; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000917:Antibody Formation / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology; D003881:Dermatomycoses / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007111:Immunity, Cellular / Q000276:immunology*; D045726:Metalloproteases / Q000032:analysis / Q000276:immunology*; D008864:Microsporum / Q000201:enzymology* / Q000276:immunology; D010447:Peptide Hydrolases / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D011994:Recombinant Proteins; D045726:Metalloproteases; D010447:Peptide Hydrolases; C035855:keratinase