Cryopreservation of spermatozoa from closed colonies, and inbred, spontaneous mutant, and transgenic strains of rats.
We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F3441 DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196 degrees C. After thawing at 37 degrees C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.
Nakatsukasa
Ena
E
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Kashiwazaki
Naomi
N
Takizawa
Akiko
A
Shino
Masao
M
Kitada
Kazuhiro
K
Serikawa
Tadao
T
Hakamata
Yoji
Y
Kobayashi
Eiji
E
Takahashi
Riichi
R
Ueda
Masatsugu
M
Nakashima
Tatsuyuki
T
Nakagata
Naomi
N
D000818:Animals; D030801:Animals, Genetically Modified; D001947:Breeding; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005298:Fertility / Q000502:physiology*; D005328:Fetal Viability / Q000502:physiology; D007315:Insemination, Artificial; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D051381:Rats; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains; D012662:Semen Preservation; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa