title: Determination of glutathione peroxidase activity in human milk.
abstract: An analytical method for determining glutathione peroxidase (GPx) (EC 1.11.1.9) activity in whole blood has been adapted to human milk samples. The values obtained for precision (relative standard deviation: 8.4%), linearity and accuracy (recovery: 90.4%) indicate the adequacy of the method for the mentioned purpose. The method was applied to 11 human milk samples obtained in the range from 6 to 135 days post partum. The resulting GPx activities (83.3 +/- 23.6 U/L) did not correlate to the selenium content of the samples, though a reciprocal correlation was found between the duration of lactation and milk GPx activity.
authors.affiliation: Nutrition and Food Chemistry Faculty of Pharmacy, Valencia University, Burjassot, Valencia, Spain.
authors.forename: Angeles
authors.initials: A
authors.lastname: Torres
authors.forename: Rosaura
authors.initials: R
authors.lastname: Farré
authors.forename: Maria Jesús
authors.initials: MJ
authors.lastname: Lagarda
authors.forename: Javier
authors.initials: J
authors.lastname: Monleón
mesh_terms: D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D007774:Lactation / Q000502:physiology; D008895:Milk, Human / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
chemical_list: D005979:Glutathione Peroxidase