title: Application of molecular beam deflection time-of-flight mass spectrometry to peptide analysis.
abstract: The application of molecular beam deflection time-of-flight mass spectrometry (MBD-TOFMS) to peptide identification is described. The technique permits a simultaneous measurement of molecular mass and electric dipole susceptibility. The mass and susceptibility are not strongly correlated, and the results can be presented as a two-dimensional map. The susceptibility provides a useful way to disperse isobaric and isomeric peptides, and at least for small peptides, the susceptibility is significantly different for different amino acid sequences. Results for peptides in the mass range 1000-2300 Da show that the mass and susceptibility lead to a higher identification score than mass spectra alone.
authors.affiliation: Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579, Université Lyon I et CNRS, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. antoine@lasim.univ-lyon1.fr
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Antoine
authors.forename: Isabelle
authors.initials: I
authors.lastname: Compagnon
authors.forename: Driss
authors.initials: D
authors.lastname: Rayane
authors.forename: Michel
authors.initials: M
authors.lastname: Broyer
authors.forename: Philippe
authors.initials: P
authors.lastname: Dugourd
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sommerer
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.forename: David
authors.initials: D
authors.lastname: Pippen
authors.forename: Frederick C
authors.initials: FC
authors.lastname: Hagemeister
authors.forename: Martin F
authors.initials: MF
authors.lastname: Jarrold
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D021542:Amino Acids, Neutral / Q000737:chemistry; D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D004151:Dipeptides / Q000032:analysis; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006096:Gramicidin / Q000032:analysis; D007986:Luteinizing Hormone / Q000032:analysis; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011487:Protein Conformation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D021542:Amino Acids, Neutral; D004151:Dipeptides; D010455:Peptides; D000804:Angiotensin II; D006096:Gramicidin; D007986:Luteinizing Hormone; D000803:Angiotensin I