title: The diversity of vacA and cagA genes of Helicobacter pylori in East Asia.
abstract: It has been reported that Helicobacter pylori infection with the type I strain, which expresses the VacA and CagA antigens, is associated with duodenal ulcer. We examined the diversity of vacA and cagA genes in 143 isolates obtained from patients with duodenal ulcer or chronic gastritis in East Asia (two different areas of Japan, Fukui and Okinawa, and also in Hangzhou, China) by polymerase chain reaction (PCR) and sequence analysis. Diversities of cagA and vacA genes were detected in East Asia. The prevalence of cagA-positive H. pylori was significantly different between Fukui and Okinawa (P=0.0032). The prevalence of Western type CagA was significantly higher in Okinawa than in Fukui (P<0.0001). However, there was no significant association between the genotype of cagA and clinical outcome. In Japan, the predominant vacA genotype was s1c/m1b. In contrast, in Hangzhou, the predominant vacA genotype was s1c/m2, and they were all East Asian CagA-positive. These findings suggest that a distinct distribution of the vacA and cagA genotypes is present in East Asia, regardless of clinical outcome.
authors.affiliation: Second Department of Internal Medicine, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.forename: Shiho
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Akiyo
authors.initials: A
authors.lastname: Yamakawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohtani
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Keida
authors.forename: Hideaki
authors.initials: H
authors.lastname: Higashi
authors.forename: Masanori
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Jianmin
authors.initials: J
authors.lastname: Si
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
mesh_terms: D000328:Adult; D000483:Alleles; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002681:China; D004381:Duodenal Ulcer / Q000382:microbiology*; D005202:Asia, Eastern; D005260:Female; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori