title: Mutations including IS6110 insertion in the gene encoding the MPB64 protein of Capilia TB-negative Mycobacterium tuberculosis isolates.
abstract: A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.
authors.affiliation: Department of Basic Research, Research Institute of Tuberculosis, Fukujuji Hospital, Kiyose-shi, Tokyo 204-8533, Japan. hirano@jata.or.jp
authors.forename: Kazue
authors.initials: K
authors.lastname: Hirano
authors.forename: Akio
authors.initials: A
authors.lastname: Aono
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Chiyoji
authors.initials: C
authors.lastname: Abe
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002845:Chromatography; D004251:DNA Transposable Elements; D016254:Mutagenesis, Insertional; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C080865:MPB64 protein, Mycobacterium