title: [Study on DNA polymerase beta gene mutation in human cervical cancer].
abstract: OBJECTIVE
  To investigate whether DNA polymerase beta (POLB) gene mutations occur in human cervical cancer (CC).
  
  
  METHODS
  To collect fresh specimens from 34 cervical cancer and examine the mutation of POLB gene using reverse transcription polymerase chain reaction (RT-PCR), single strand conformation polymorphism (SSCP) analysis and sequence analysis. According to their histological grading, the 34 cases of CC were divided into three groups: 9 cases of grade I (G(1)), 14 cases of grade II (G(2)), 11 cases of grade III (G(3)).
  
  
  RESULTS
  POLB mutations were detected in the tissues of CC. The mutation of POLB is related with the histological differentiated of the CC. The mutated rates in low differentiated cancer were significantly higher than those in moderate and high ones, and the difference was significant (P < 0.05). The sequencing of the PCR product showed an A to G chang at nucleotide 660, which resulted in a substitution of the amino acid from arginine to glycin at codon 182.
  
  
  CONCLUSIONS
  There were POLB mutations in the tissues of CC, and this may be related with the development of the CC. The mutations impaired the catalytic activity of POLB. As a result, this may lead to observed accumulation of mutations in tumor cells.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Han
authors.forename: Yu-huan
authors.initials: YH
authors.lastname: Qiao
authors.forename: Zi-ming
authors.initials: ZM
authors.lastname: Dong
authors.forename: Hui-rong
authors.initials: HR
authors.lastname: Shi
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Zhao
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
mesh_terms: D019951:DNA Polymerase beta / Q000235:genetics*; D005260:Female; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology
chemical_list: D019951:DNA Polymerase beta