title: Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus.
abstract: Resistin is an adipocyte-derived peptide that might play a role in obesity and insulin resistance. However, its role in humans is largely unclear. Although many studies have measured the expression of human resistin in tissues, the circulating concentrations of resistin and its relation to metabolic parameters in humans are unknown. We developed an ELISA for human resistin and measured plasma concentrations in aged individuals with or without type 2 diabetes mellitus. To validate the results of plasma resistin concentrations in our subjects, plasma adiponectin concentrations were also determined, which were higher in nondiabetic subjects than in type 2 diabetic patients and correlated with the homeostasis model assessment for insulin resistance (HOMA-IR). Log-transformed plasma resistin concentrations (log-resistin) were higher in diabetic patients compared with normal individuals (0.50 +/- 0.39 vs. 0.28 +/- 0.51 ng/ml; P < 0.001), and this difference was significant after controlling for gender and body mass index. Log-resistin did not show a significant correlation with HOMA-IR, waist circumference, body mass index, blood pressure, or total cholesterol. The plasma glucose concentration was an independent factor associated with log-resistin. In conclusion, plasma resistin concentrations are elevated in patients with type 2 diabetes, but are not associated with insulin resistance or obesity.
authors.affiliation: KOMED Institute for Life Science, Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea.
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Youn
authors.forename: Kang-Yeol
authors.initials: KY
authors.lastname: Yu
authors.forename: Hong Je
authors.initials: HJ
authors.lastname: Park
authors.forename: Nam Seok
authors.initials: NS
authors.lastname: Lee
authors.forename: Sung Shik
authors.initials: SS
authors.lastname: Min
authors.forename: Moon Yeon
authors.initials: MY
authors.lastname: Youn
authors.forename: Young Min
authors.initials: YM
authors.lastname: Cho
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Park
authors.forename: Seong Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Hong Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D001824:Body Constitution; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006706:Homeostasis; D006729:Hormones, Ectopic / Q000097:blood*; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D052243:Resistin
chemical_list: D000911:Antibodies, Monoclonal; D001786:Blood Glucose; D006729:Hormones, Ectopic; C499624:RETN protein, human; D052243:Resistin; D002784:Cholesterol