title: Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics.
abstract: Alumina ceramics have excellent mechanical and biocompatible properties, but are bioinert and hence have no bone-bonding properties. We took a tissue-engineering approach in an attempt to modify the ceramic surface and so provide an osteogenic/osteoconductive milieu. We obtained human bone marrow mesenchymal cells from four donors and then cultured the cells for two weeks on alumina ceramic in the presence of beta-glycerophosphate, ascorbic acid and dexamethasone. The cells showed extensive alkaline phosphatase staining and mineralization, as evidenced by Alizarin Red S staining and calcein uptake. Biochemical analyses revealed high levels of alkaline phosphatase activity, osteocalcin expression and calcium content. This data indicates the appearance of active osteoblasts that are concomitant with bone matrix formation, i.e., in vitro cultured bone. The cultured bone/alumina composites should prevent the aseptic loosening of all-alumina ceramic joints or the detachment of implanted alumina ceramics, and thus could have clinical significance in orthopedic reconstructive surgery.
authors.affiliation: Department of Orthopedic Surgery, Nippon Medical School, Tokyo, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kitamura
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Funaoka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takakura
authors.forename: Hiromoto
authors.initials: H
authors.lastname: Ito
mesh_terms: D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D000537:Aluminum Oxide; D001854:Bone Marrow Cells / Q000166:cytology*; D002118:Calcium / Q000032:analysis; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015675:Osteocalcin; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide; D002118:Calcium