[Detection of carbonic anhydrase IX gene expression in renal cell carcinoma with reverse transcription-PCR].
OBJECTIVE
To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC).


METHODS
MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis.


RESULTS
Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues.


CONCLUSIONS
RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.
Jiang
Yao-dong
YD
Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jyd@fimmu.com
Zheng
Shao-bin
SB
Wang
Zhan-hui
ZH
D000951:Antigens, Neoplasm / Q000235:genetics*; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D000951:Antigens, Neoplasm; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases