Radiation sterilisation of cultured human brain tumour cells for clinical immune tumour therapy.
The aim is to investigate the radiosensitivity of noninfected cultured human glioma cells to ascertain that intracutaneously administered cells are viable enough to produce interferon-gamma but not able to proliferate. Cell cultures were established from five patients undergoing brain tumour surgery. By karyotyping, we found four malignant (three glioblastoma multiforme (GBM), one giant cell glioma) and one normal. The cells were irradiated with (137)Cs-gamma rays at absorbed dose levels of 0, 20, 40, 60, 80, 100 and 120 Gy. The fraction of viable cells was examined by MTT incorporation assay. The average of the data obtained from three GBM cell cultures was fitted to an exponential model. The parameters were: extrapolation number n=0.85+/-0.10, mean lethal dose D(0)=12.4+/-3.2 Gy and an additional uncertainty parameter deltaS=0.14+/-0.03. By setting deltaS=0, the corresponding values of the parameters were n=0.86+/-0.16 and D(0)=30.0+/-8.1 Gy. The rate of proliferation was examined by (3)H-thymidine incorporation. The average of the proliferation data obtained from three GBM cell cultures was fitted to an exponential model yielding n=0.943+/-0.005 and D(0)=5.8+/-0.5 Gy for deltaS=0.057+/-0.005, and by setting deltaS=0, n=1.00+/-0.02 and D(0)=8.4+/-1.6 Gy. No outgrowth of plated cells was observed after 4 weeks at an absorbed dose of 100 Gy. This absorbed dose is recommended for irradiation of 2 x 10(6) glioma cells used for clinical immunisation.
Bauréus-Koch
C
C
Department of Radiation Physics, Lund University Hospital, Klinikgatan 7, SE 221 85 Lund, Sweden.
Nyberg
G
G
Widegren
B
B
Salford
L G
LG
Persson
B R R
BR
D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division; D002470:Cell Survival; D002588:Cesium Radioisotopes / Q000627:therapeutic use; D015316:Genetic Therapy / Q000379:methods*; D005909:Glioblastoma / Q000473:pathology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007167:Immunotherapy; D007371:Interferon-gamma / Q000096:biosynthesis*; D011836:Radiation Tolerance; D013242:Sterilization / Q000379:methods; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured
D002588:Cesium Radioisotopes; D014316:Tritium; D007371:Interferon-gamma; D013936:Thymidine