Expression of intercellular adhesion molecule-1 (ICAM- 1) during ischemia-reperfusion in human liver tissue allograft: image analysis by confocal laser scanning microscopy.
We studied and quantified the effect of ischemia-reperfusion on expression of intercellular adhesion molecule-1 (ICAM-1) on sinusoidal endothelial cells and hepatocyte plasma membranes by means of confocal laser scanning microscopy imaging. We found that ischemia induced an increase in ICAM-1 expression on sinusoidal endothelial cells and hepatocytes. After reperfusion, ICAM-1 expression was increased on sinusoidal endothelial cells, whereas it was unmodified on hepatocytes. On the other hand, ICAM-1 expression was not correlated to ischemia-reperfusion liver injury. Therefore, hepatocellular ischemia-reperfusion injury could be induced by other causes than immune-mediated damages.
Benkoel
Liliane
L
INSERM U 559, Faculté de Médecine, 27 boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Dodero
Frank
F
Hardwigsen
Jean
J
Benoliel
Anne Marie
AM
Bongrand
Pierre
P
Botta-Fridlund
Danielle
D
Le Treut
Yves Patrice
YP
Chamlian
Albert
A
Lombardo
Dominique
D
D000328:Adult; D002462:Cell Membrane / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D018799:Intercellular Adhesion Molecule-1