Restriction enzyme-generated siRNA (REGS) vectors and libraries.
Small interfering RNA (siRNA) technology facilitates the study of loss of gene function in mammalian cells and animal models, but generating multiple siRNA vectors using oligonucleotides is slow, inefficient and costly. Here we describe a new, enzyme-mediated method for generating numerous functional siRNA constructs from any gene of interest or pool of genes. To test our restriction enzyme-generated siRNA (REGS) system, we silenced a transgene and two endogenous genes and obtained the predicted phenotypes. REGS generated on average 34 unique siRNAs per kilobase of sequence. REGS enabled us to create enzymatically a complex siRNA library (>4 x 10(5) clones) from double-stranded cDNA encompassing known and unknown genes with 96% of the clones containing inserts of the appropriate size.
Sen
George
G
Department of Molecular Pharmacology, 269 Campus Drive, CCSR 4225A Stanford University School of Medicine, Stanford, California 94305, USA.
Wehrman
Tom S
TS
Myers
Jason W
JW
Blau
Helen M
HM
D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D020868:Gene Silencing / Q000502:physiology; D005822:Genetic Vectors; D015698:Genomic Library; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*
D034741:RNA, Small Interfering; D004262:DNA Restriction Enzymes