title: [Role of tissue factor in the invasive ability of cultured human colon carcinoma cells].
abstract: OBJECTIVE
  To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT-29).
  
  
  METHODS
  The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT-29 cells with sense/antisense TFcDNA transfection.
  
  
  RESULTS
  HT-29 cells with sense-TFcDNA transfection showed increased TF expression and invasive ability compared with the cells without transfection, but HT-29 cells with antisense-TFcDNA transfection got the contrary change.
  
  
  CONCLUSIONS
  TF can increase the invasive ability of HT-29 cells in vitro.
authors.affiliation: Department of General Surgery Peking University First Hospital Beijing 100034 China. docyhw@sina.com
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yao
authors.forename: Yuanlian
authors.initials: Y
authors.lastname: Wan
authors.forename: Jingming
authors.initials: J
authors.lastname: Ye
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ju
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D003110:Colonic Neoplasms / Q000473:pathology*; D016373:DNA, Antisense / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000502:physiology*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D013925:Thromboplastin