[A study of aspermia-related genes by genchips and analysis of the RAP1A gene].
OBJECTIVE
To study the differential gene expression profiles between the normal and aspermia human testes by genechips.


METHODS
Probes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H-40s genechips to detect the differential gene expression profiles. A distinctly up-regulated gene RAP1A was analyzed by bibliogrphic retrieval.


RESULTS
Six hundred and twenty-three differential expressed genes were found, among which the distinctly up-regulated gene RAP1A was closely related to human sperm regulation.


CONCLUSIONS
Screening the differential gene expression profiles between the normal and aspermia human testes by genechips can be used in the study of aspermia-related genes.
Yang
Bo
B
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Gao
Xiao-Kang
XK
Wang
He
H
Liu
He-Liang
HL
Chen
Bao-Qi
BQ
Yuan
Jian-Lin
JL
Shao
Guo-Xing
GX
Shao
Chen
C
D000328:Adult; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009845:Oligospermia / Q000235:genetics*; D020827:rap1 GTP-Binding Proteins / Q000235:genetics*
D020827:rap1 GTP-Binding Proteins