Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates.
Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.
Kaul
Zeenia
Z
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 3058562, Japan.
Yaguchi
Tomoko
T
Kaul
Sunil C
SC
Hirano
Takashi
T
Wadhwa
Renu
R
Taira
Kazunari
K
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology; D019187:Cadmium Compounds / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D002477:Cells / Q000166:cytology / Q000378:metabolism*; D003116:Color; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008163:Luminescent Measurements; D024101:Mitochondrial Proteins; D036103:Nanotechnology; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D055095:Optics and Photonics; D010316:Particle Size; D011789:Quantum Theory; D012509:Sarcoma / Q000378:metabolism / Q000473:pathology; D018036:Selenium Compounds / Q000737:chemistry; D019809:Streptavidin / Q000276:immunology
D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D018840:HSP70 Heat-Shock Proteins; C492001:HSPA9 protein, human; D008024:Ligands; D024101:Mitochondrial Proteins; D018036:Selenium Compounds; C080352:mortalin; D019809:Streptavidin