title: Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.
abstract: A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yoh
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Hirata
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Takabatake
authors.forename: Sabine
authors.initials: S
authors.lastname: Bork
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001403:Babesia / Q000235:genetics* / Q000276:immunology; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017125:Genes, Protozoan; D006734:Horse Diseases / Q000175:diagnosis* / Q000276:immunology; D006736:Horses; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins