title: Topoisomerase I dissociates human immunodeficiency virus type 1 reverse transcriptase from genomic RNAs.
abstract: Both HIV-1 reverse transcriptase (RT) and topoisomerase I bind to structural RNAs and they cooperate to synthesize cDNA during the replication of HIV-1. In this study, we find that human topoisomerase I exclusively dissociated HIV-1 reverse transcriptase, which strongly binds to structural RNAs. Meanwhile, topoisomerase I did not dissociate either HIV-1 nucleocapsid proteins or murine leukemia virus RT which was bound to RNA. We propose that human topoisomerase I may regulate the binding of RT to RNAs and play a pivotal role in HIV-1 replication.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. htakahas@nih.go.jp
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagashima
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurata
mesh_terms: D001483:Base Sequence; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000235:genetics / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000201:enzymology; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020038:RNA, Spliced Leader / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014779:Virus Replication
chemical_list: D015683:Gene Products, gag; D019590:Nucleocapsid Proteins; D020038:RNA, Spliced Leader; D011994:Recombinant Proteins; D012313:RNA; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D004264:DNA Topoisomerases, Type I