Direct IBD mapping: identical-by-descent mapping without genotyping.
Direct identical-by-descent (IBD) mapping is a technique, that combines genomic mismatch scanning (GMS) and DNA microarray technology, for mapping regions shared IBD between two individuals without locus-by-locus genotyping or sequencing. The lack of reagents has limited its widespread application. In particular, two key reagents have been limiting, 1). mismatch repair proteins MutS, L and H, and 2). genomic microarrays for identifying the genomic locations of the GMS-selected IBD fragments. Here, we describe steps that optimized the procedure and resources that will facilitate the development of direct IBD mapping.
Smirnov
Denis
D
Department of Genetics, University of Pennsylvania, Philadelphia, PA 19104, USA.
Bruzel
Alan
A
Morley
Michael
M
Cheung
Vivian G
VG
D020137:Base Pair Mismatch; D002874:Chromosome Mapping; D005192:Family Health; D005826:Genetics, Medical / Q000379:methods; D015894:Genome, Human; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis