title: Acyl-CoA dehydrogenases and acyl-CoA oxidases. Structural basis for mechanistic similarities and differences.
abstract: Acyl-CoA dehydrogenases and acyl-CoA oxidases are two closely related FAD-containing enzyme families that are present in mitochondria and peroxisomes, respectively. They catalyze the dehydrogenation of acyl-CoA thioesters to the corresponding trans-2-enoyl-CoA. This review examines the structure of medium chain acyl-CoA dehydrogenase, as a representative of the dehydrogenase family, with respect to the catalytic mechanism and its broad chain length specificity. Comparing the structures of four other acyl-CoA dehydrogenases provides further insights into the structural basis for the substrate specificity of each of these enzymes. In addition, the structure of peroxisomal acyl-CoA oxidase II from rat liver is compared to that of medium chain acyl-CoA dehydrogenase, and the structural basis for their different oxidative half reactions is discussed.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA. jjkim@mcw.edu
authors.forename: Jung-Ja P
authors.initials: JJ
authors.lastname: Kim
authors.forename: Retsu
authors.initials: R
authors.lastname: Miura
mesh_terms: D044944:Acyl-CoA Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D042962:Acyl-CoA Oxidase / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D044944:Acyl-CoA Dehydrogenases; D042962:Acyl-CoA Oxidase