title: A map of the interactome network of the metazoan C. elegans.
abstract: To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.
authors.affiliation: Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Armstrong
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertin
authors.forename: Hui
authors.initials: H
authors.lastname: Ge
authors.forename: Stuart
authors.initials: S
authors.lastname: Milstein
authors.forename: Mike
authors.initials: M
authors.lastname: Boxem
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Vidalain
authors.forename: Jing-Dong J
authors.initials: JD
authors.lastname: Han
authors.forename: Alban
authors.initials: A
authors.lastname: Chesneau
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Debra S
authors.initials: DS
authors.lastname: Goldberg
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Monica
authors.initials: M
authors.lastname: Martinez
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Rual
authors.forename: Philippe
authors.initials: P
authors.lastname: Lamesch
authors.forename: Lai
authors.initials: L
authors.lastname: Xu
authors.forename: Muneesh
authors.initials: M
authors.lastname: Tewari
authors.forename: Sharyl L
authors.initials: SL
authors.lastname: Wong
authors.forename: Lan V
authors.initials: LV
authors.lastname: Zhang
authors.forename: Gabriel F
authors.initials: GF
authors.lastname: Berriz
authors.forename: Laurent
authors.initials: L
authors.lastname: Jacotot
authors.forename: Philippe
authors.initials: P
authors.lastname: Vaglio
authors.forename: Jérôme
authors.initials: J
authors.lastname: Reboul
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hirozane-Kishikawa
authors.forename: Qianru
authors.initials: Q
authors.lastname: Li
authors.forename: Harrison W
authors.initials: HW
authors.lastname: Gabel
authors.forename: Ahmed
authors.initials: A
authors.lastname: Elewa
authors.forename: Bridget
authors.initials: B
authors.lastname: Baumgartner
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Rose
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Yu
authors.forename: Stephanie
authors.initials: S
authors.lastname: Bosak
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Sequerra
authors.forename: Andrew
authors.initials: A
authors.lastname: Fraser
authors.forename: Susan E
authors.initials: SE
authors.lastname: Mango
authors.forename: William M
authors.initials: WM
authors.lastname: Saxton
authors.forename: Susan
authors.initials: S
authors.lastname: Strome
authors.forename: Sander
authors.initials: S
authors.lastname: Van Den Heuvel
authors.forename: Fabio
authors.initials: F
authors.lastname: Piano
authors.forename: Jean
authors.initials: J
authors.lastname: Vandenhaute
authors.forename: Claude
authors.initials: C
authors.lastname: Sardet
authors.forename: Mark
authors.initials: M
authors.lastname: Gerstein
authors.forename: Lynn
authors.initials: L
authors.lastname: Doucette-Stamm
authors.forename: Kristin C
authors.initials: KC
authors.lastname: Gunsalus
authors.forename: J Wade
authors.initials: JW
authors.lastname: Harper
authors.forename: Michael E
authors.initials: ME
authors.lastname: Cusick
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Roth
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D019143:Evolution, Molecular; D017238:Genes, Helminth; D023281:Genomics; D016366:Open Reading Frames; D010641:Phenotype; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: D029742:Caenorhabditis elegans Proteins; D020543:Proteome