title: [Effect of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD34+ CD38- hematopoietic primitive progenitor cells].
abstract: OBJECTIVE
  To investigate the effects of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD(34)(+) hematopoietic stem/progenitor cells in cord blood.
  
  
  METHODS
  Retroviral vectors containing Tpo or IL-11 gene were constructed and used to transfect the stromal cell line HFCL. Tpo and/or IL-11 mRNA was assayed by Northern blot. Non-modified stromal cells were used, CD(34)(+) hematopoietic stem/progenitor cells from cord blood were expanded on gene-modified stromal cells for 7 days. The phenotype of CD(34)(+)CD(38)(-) primitive progenitors was detected by flow cytometry.
  
  
  RESULTS
  HFCL expressed Tpo and/or IL-11 mRNA after transfected by the retroviral vectors. The percentages of CD(34)(+)CD(38)(-) primitive progenitors in the cultures of Tpo, IL-11 and Tpo + IL-11 modified HFCL were (1.8 +/- 0.24)%, (1.62 +/- 0.23)%, and (2.45 +/- 0.28)%, respectively, which were higher than that in the control [(0.8 +/- 0.23)%].
  
  
  CONCLUSIONS
  The stromal cells modified by Tpo and/or IL-11 gene were able to enhance ex vivo expansion of CD(34)(+) and CD(34)(+)CD(38)(-) hematopoietic stem/progenitor cells from cord blood.
authors.affiliation: Chongqing Children's Hospital, Chongqing 400014, China.
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Lu
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Pei
authors.forename: Ya-de
authors.initials: YD
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.forename: Ci-xian
authors.initials: CX
authors.lastname: Bai
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000032:analysis*; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34 / Q000032:analysis*; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D017370:Interleukin-11 / Q000235:genetics*; D008562:Membrane Glycoproteins; D017154:Stromal Cells / Q000502:physiology; D013926:Thrombopoietin / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D017370:Interleukin-11; D008562:Membrane Glycoproteins; D013926:Thrombopoietin; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1