title: Streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs.
abstract: Canine intestinal lactic acid bacterium (LAB) population in four fistulated dogs was cultured and enumerated using MRS agar. LAB levels ranging from 1.4x10(6) to 1.5x10(7) CFU ml(-1) were obtained in jejunal chyme. In the fecal samples 7.0x10(7) and 2.0x10(8) CFU g(-1) were detected. Thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16S and 23S rDNA restriction fragment length polymorphism patterns (ribotyping) and 16S rDNA sequence analysis. According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both feces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected.
authors.affiliation: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, P.O. Box 57, University of Helsinki, Finland. minna.rinkinen@helsinki.fi
authors.forename: Minna L
authors.initials: ML
authors.lastname: Rinkinen
authors.forename: Joanna M K
authors.initials: JM
authors.lastname: Koort
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: Elias
authors.initials: E
authors.lastname: Westermarck
authors.forename: K Johanna
authors.initials: KJ
authors.lastname: Björkroth
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D004285:Dogs; D005243:Feces / Q000382:microbiology*; D005402:Fistula; D007583:Jejunum / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid