Protein product encoded by a human novel gene E9730 enhances AP-1 activity through interacting with Jab1.
A novel human gene, named E9730 (a clone number of fetal liver cDNA library), has been identified from more than 14,000 expressed sequence tags (ESTs) based on our large scale sequencing of human fetal liver cDNA libraries. Although sequencing of this novel human gene indicates that it is a leucine zipper protein, the function of E9730 and its homongous genes among species is unknown yet. To find out physiological functional clue of E9730, the yeast two-hybrid system was used to screen the E9730-interacting protein(s), and one clone containing a cDNA insert with almost the entire coding sequence (amino acids 39 C335) of human Jab1 (Jun-activating domain binding protein 1) that interacted specifically with E9730 was identified. A specific association between Jab1 and E9730 was shown by co-immunoprecipitation and co-localization experiments. Furthermore, the data indicated that E9730 appeared to enhance Jab1-induced AP-1 activity in a concentration-dependent manner and Jab1 may be involved in the intracellular signaling transduction from E9730 to AP-1.
Wang
Zhao-Qing
ZQ
Department of System Biology, Beijing Institute of Radiation Medicine, Chinese Human National Center at Beijing, Beijing 100850, China. hefc@nic.bmi.ac.cn
Wei
Han-Dong
HD
He
Fu-Chu
FC
D000595:Amino Acid Sequence; D000818:Animals; D000075686:COP9 Signalosome Complex; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000196:embryology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C484587:E9730 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D010447:Peptide Hydrolases; C501488:COPS5 protein, human; D000075686:COP9 Signalosome Complex