title: pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis.
abstract: Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.
authors.affiliation: Department of Radiology, Okayama University Medical School, Okayama 700-8558, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kuroda
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wang
authors.forename: Shiro
authors.initials: S
authors.lastname: Akaki
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Asaumi
authors.forename: Kohichi
authors.initials: K
authors.lastname: Shibuya
authors.forename: Shoji
authors.initials: S
authors.lastname: Kawasaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Okumura
authors.forename: Susumu
authors.initials: S
authors.lastname: Kanazawa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hiraki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis / Q000276:immunology / Q000502:physiology*; D002467:Cell Nucleus / Q000276:immunology / Q000502:physiology*; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D013997:Time Factors; D019014:fas Receptor / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D019014:fas Receptor