title: A naturally occurring -263G/C variant of the human AA-NAT gene and overnight melatonin production.
abstract: Several lines of evidence show that the daily amount of melatonin produced differs greatly between individuals. Any polymorphism in the gene of arylalkylamine N-acetyltransferase (AA-NAT), a critical enzyme involved in melatonin biosynthesis, may contribute to the variability of melatonin production. The present study investigated the possible association between overnight melatonin excretion and a commonly occurring -263G/C polymorphism in the promoter region of the human AA-NAT gene. However, we found that -263G/C variant had no effect on the overnight 6-OHMS excretion. In this study, individual genotyping for -263G/C was determined by denaturing high performance liquid chromatography (DHPLC) and confirmed by sequencing. The overnight urinary 6-hydroxymelatonin sulfate (6-OHMS) excretion was quantified by enzyme-linked immunosorbent assay (ELISA).
authors.affiliation: Department of Pharmacology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore.
authors.forename: Grace Wang
authors.initials: GW
authors.lastname: Ying
authors.forename: Caroline Guat Lay
authors.initials: CG
authors.lastname: Lee
authors.forename: Edmund Jon Deoon
authors.initials: EJ
authors.lastname: Lee
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D001191:Arylamine N-Acetyltransferase; D008550:Melatonin; C012365:6-hydroxymelatonin