title: The yeast Zygosaccharomyces bailii: a new host for heterologous protein production, secretion and for metabolic engineering applications.
abstract: Molecular tools for the production of heterologous proteins and metabolic engineering applications of the non-conventional yeast Zygosaccharomyces bailii were developed. The combination of Z. bailii's resistance to relatively high temperature, osmotic pressure and low pH values, with a high specific growth rate renders this yeast potentially interesting for exploitation for biotechnological purposes as well as for the understanding of the biological phenomena and mechanisms underlying the respective resistances. Looking forward to these potential applications, here we present the tools required for the production and the secretion of different heterologous proteins, and one example of a metabolic engineering application of this non-conventional yeast, employing the newly developed molecular tools.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Branduardi
authors.forename: Minoska
authors.initials: M
authors.lastname: Valli
authors.forename: Luca
authors.initials: L
authors.lastname: Brambilla
authors.forename: Michael
authors.initials: M
authors.lastname: Sauer
authors.forename: Lilia
authors.initials: L
authors.lastname: Alberghina
authors.forename: Danilo
authors.initials: D
authors.lastname: Porro
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D020068:Zygosaccharomyces / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D001616:beta-Galactosidase