title: [Experimental study in vitro on toxicology and cytocompatibility of collagen/hydroxylapatite (CHA) composite material as part of tracheal prosthesis].
abstract: Cytotoxicity and cytocompatibility remains the principal theme for biomaterials application in medicine. The purpose of this study was to investigate the cytotoxicity and cytocompatibility of collagen/hydroxyapatite(CHA) composite material in vitro in order to provide useful scientific basis for clinical use. Cellular cultivation in vitro and MTT assay were conducted for evaluating the composite material's influence on the morphology, growth and proliferation of cultured cell(L-929 cell). The hemolysis test was also performed for evaluating the impact on the function and metabolism of erythrocyte. These results demonstrated that the CHA composite material had no cytotoxicity and no hemolytic effect, and it might not be harmful to the morphology of the L-929 cell. The growth and proliferation of the L-929 cell could not be inhibited significantly. The cytotoxicity score of the composite material was grade 0. The hemolysis rate was 1.85%. In conclusion, collagen/hydroxyapatite (CHA) composite material might have good cytocompatibility and be safe for clinical use.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003. shcan111@yahoo.com.cn
authors.forename: Hongcan
authors.initials: H
authors.lastname: Shi
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Xu
authors.forename: Xiong
authors.initials: X
authors.lastname: Qin
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhao
authors.forename: Kang
authors.initials: K
authors.lastname: Sun
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002478:Cells, Cultured; D003094:Collagen / Q000633:toxicity*; D006882:Hydroxyapatites / Q000633:toxicity*; D051379:Mice; D019736:Prostheses and Implants; D018675:Toxicity Tests; D014132:Trachea
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; D003094:Collagen