Dedifferentiation of lineage-committed cells by a small molecule.
Combinatorial libraries were screened for molecules that induce mouse myogenic lineage committed cells to dedifferentiate in vitro. A 2,6-disubstituted purine, reversine, was discovered that induces lineage reversal of C2C12 cells to become multipotent progenitor cells which can redifferentiate into osteoblasts and adipocytes. This and other such molecules are likely to provide new insights into the molecular mechanisms that control cellular dedifferentiation and may ultimately be useful to in vivo stem cell biology and therapy.
Chen
Shuibing
S
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Zhang
Qisheng
Q
Wu
Xu
X
Schultz
Peter G
PG
Ding
Sheng
S
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D019070:Cell Lineage; D051379:Mice; D008970:Molecular Weight; D009025:Morpholines / Q000737:chemistry / Q000494:pharmacology*; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects; D011687:Purines / Q000737:chemistry / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
D009025:Morpholines; D011687:Purines; C484369:2-(4-morpholinoanilino)-6-cyclohexylaminopurine