Expression, reconstitution, and mutation of recombinant Streptomycescoelicolor NiSOD.
Nickel-dependent superoxide dismutases (NiSODs) represent a novel solution to controlling the deleterious effects of reactive oxygen species derived from superoxide in biology. The expression of recombinant Streptomyces coelicolor NiSOD and its in vitro processing and reconstitution to yield fully active enzyme is reported. The results of studies of NiSODs involving mutations in two putative nickel binding ligands are also reported. These studies show that mutation of M28, a strictly conserved residue and one of only three S-donor ligands in the enzyme, has no measurable effect on the spectroscopic or catalytic properties of the enzyme. In contrast, mutation of the strictly conserved N-terminal H residue has dramatic effects on both the spectroscopic and catalytic properties. These results provide insights into structural and mechanistic aspects of the novel nickel-containing reactive site.
Bryngelson
Peter A
PA
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Arobo
Sumonu E
SE
Pinkham
Jennifer L
JL
Cabelli
Diane E
DE
Maroney
Michael J
MJ
D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D009532:Nickel / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D011994:Recombinant Proteins; D009532:Nickel; D013482:Superoxide Dismutase