The planar cell polarity protein Strabismus promotes Pins anterior localization during asymmetric division of sensory organ precursor cells in Drosophila.
Cell fate diversity is generated in part by the unequal segregation of cell-fate determinants during asymmetric cell division. In the Drosophila bristle lineage, the sensory organ precursor (pI) cell is polarized along the anteroposterior (AP) axis by Frizzled (Fz) receptor signaling. We show here that Fz localizes at the posterior apical cortex of the pI cell prior to mitosis, whereas Strabismus (Stbm) and Prickle (Pk), which are also required for AP polarization of the pI cell, co-localize at the anterior apical cortex. Thus, asymmetric localization of Fz, Stbm and Pk define two opposite cortical domains prior to mitosis of the pI cell. At mitosis, Stbm forms an anterior crescent that overlaps with the distribution of Partner of Inscuteable (Pins) and Discs-large (Dlg), two components of the anterior Dlg-Pins-Galphai complex that regulates the localization of cell-fate determinants. At prophase, Stbm promotes the anterior localization of Pins. By contrast, Dishevelled (Dsh) acts antagonistically to Stbm by excluding Pins from the posterior cortex. We propose that the Stbm-dependent recruitment of Pins at the anterior cortex of the pI cell is a novel read-out of planar cell polarity.
Bellaïche
Yohanns
Y
Ecole Normale Supérieure, UMR 8542, 46, rue d'Ulm, 75230 Paris, Cedex 05, France.
Beaudoin-Massiani
Olivia
O
Stuttem
Isabella
I
Schweisguth
François
F
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D004330:Drosophila / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D051157:Frizzled Receptors; D017344:Genes, Insect; D049452:Green Fluorescent Proteins; D060588:LIM Domain Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008938:Mitosis; D008954:Models, Biological; D008969:Molecular Sequence Data; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012679:Sense Organs / Q000166:cytology* / Q000502:physiology; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology; D014714:Vertebrates
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D060588:LIM Domain Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; C111638:Vang protein, Drosophila; C058784:fz protein, Drosophila; C121600:pk protein, Drosophila; C070350:dlg1 protein, Drosophila; D049452:Green Fluorescent Proteins