Relationship between malondialdehyde level and glutathione peroxidase activity in diabetic rats.
BACKGROUND
This investigation describes the relationship between glutathione peroxidase activities, an antioxidant enzyme, and the oxidative status in diabetic rat blood.


METHODS
Malondialdehyde level and glutathione peroxidase activity were measured by spectrophotometric techniques.


CONCLUSIONS
Malondialdehyde content in the diabetic rats group was increased compared to that in the controls [3.08+/-0.32 (mean+/-S.E.) vs. 1.15+/-0.29 mM/g hemoglobin, P>0.01]. Glutathione peroxidase activity in the diabetic rats group was increased compared to that in the control [10.27+/-1.39 (mean+/-S.E.) vs. 3.14+/-0.38 micromol NADPH/min/g hemoglobin, P>0.01]. Our results show a positive correlation between serum glutathione peroxidase and malondialdehyde concentration in diabetic rats.
Qujeq
Durdi
D
Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. dqujeq@hotmail.com
Aliakbarpour
Hamid Reza
HR
Kalavi
Khodaberdi
K
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry / Q000379:methods
D000975:Antioxidants; D001786:Blood Glucose; D008315:Malondialdehyde; D005979:Glutathione Peroxidase