Comparison of methods for sample preparation of individual rat cerebrospinal fluid samples prior to two-dimensional polyacrylamide gel electrophoresis.
Different pre-treatment methods have been compared for two-dimensional mapping of individual rat cerebrospinal fluid samples based on acetone, trichloroacetic acid/acetone and methanol/acetone precipitation of proteins. Acetone precipitation following incubation with DTT gave the highest protein recovery (72%) and the largest number of protein spots (92 +/- 4) as well as minimizing the time taken.
Garcia-Rodriguez
Sonia
S
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González s/n, 41012 Seville, Spain.
Castilla
Sandro Argüelles
SA
Machado
Alberto
A
Ayala
Antonio
A
D000096:Acetone / Q000737:chemistry*; D000818:Animals; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D011232:Chemical Precipitation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014238:Trichloroacetic Acid / Q000737:chemistry*
D002556:Cerebrospinal Fluid Proteins; D000096:Acetone; D014238:Trichloroacetic Acid