Photometric assay for measuring the intracellular concentration of branched-chain amino acids in bacteria.
The changes in intracellular pool of branched-chain amino acids (BCAA) regulate different physiological processes in bacteria. Up to date, the only available photometric test for measuring BCAA concentration was adapted for blood and plasma samples in diagnostic purposes. We have modified this method for use on bacterial cells, and tested its applicability on several model organisms: Lactococcus lactis, Bacillus subtilis and Escherichia coli.
Petranovic
Dina
D
Laboratoire de Génétique Microbienne, CRJ-INRA, F-78352 CEDEX, Jouy en Josas, France. dina@jouy.inra.fr
Mijakovic
Ivan
I
D000597:Amino Acids, Branched-Chain / Q000032:analysis*; D001412:Bacillus subtilis / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D013294:Lactococcus lactis / Q000737:chemistry*; D010783:Photometry / Q000379:methods*
D000597:Amino Acids, Branched-Chain