Effect of single dose irradiation on human glioblastoma spheroids in vitro.
Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.
Fehlauer
Fabian
F
Department of Radiation Oncology, University of Hamburg, Martinistrasse 52, 20246 Hamburg, Germany. fehlauer@uke.uni-hamburg.de
Stalpers
Lukas J A
LJ
Panayiotides
John
J
Kaaijk
Patricia
P
González González
Dionisio
D
Leenstra
Sieger
S
van der Valk
Paul
P
Sminia
Peter
P
D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D005909:Glioblastoma / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D006801:Humans; D009924:Organ Culture Techniques; D011237:Predictive Value of Tests; D016634:Radiosurgery / Q000379:methods*; D011878:Radiotherapy / Q000379:methods; D016019:Survival Analysis; D016212:Transforming Growth Factor beta / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D014408:Biomarkers, Tumor; D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A