Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes.
We report the differentiation of human adipose tissue stem cells (ATSCs) to take on cardiomyocyte properties following transient exposure to a rat cardiomyocyte extract. Reversibly permeabilized ATSCs were incubated for 1h in a nuclear and cytoplasmic extract of rat cardiomyocytes, resealed with CaCl(2), and cultured. Three weeks after exposure to extract, ATSCs expressed several cardiomyocyte markers including sarcomeric alpha-actinin, desmin, and cardiac troponin I, and displayed targeted expression of the gap junction protein connexin 43. Formation of binucleated and striated cells, and spontaneous beating in culture were also observed. A low proportion of intact ATSCs exposed to the extract also showed signs of alpha-actinin and connexin 43 expression. Additional evidence of differentiation was provided by induction of expression of nuclear lamin A/C, a marker of terminally differentiated cells, and a remarkable increase in cell cycle length. Together with our previous data, this study suggests that alteration of cell fate using cellular extracts may be applied to multiple cell types. Cell extracts may also prove useful for investigating the molecular mechanisms of stem cell differentiation.
Gaustad
Kristine G
KG
Institute of Medical Biochemistry, University of Oslo, Oslo, Norway.
Boquest
Andrew C
AC
Anderson
Brent E
BE
Gerdes
A Martin
AM
Collas
Philippe
P
D000185:Actinin / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D018031:Connexin 43 / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D014966:Xanthenes / Q000494:pharmacology
D004396:Coloring Agents; D018031:Connexin 43; D018076:DNA, Complementary; D014966:Xanthenes; D000185:Actinin; C034657:Texas red; D001973:Bromodeoxyuridine