MLH1p and MLH3p localize to precociously induced chiasmata of okadaic-acid-treated mouse spermatocytes.
With the phosphatase inhibitor, okadaic acid, we induce the precocious onset of the chiasmate stage and under those conditions show that the recombination nodules, MLH1 and MLH3 foci, are localized to the chiasmata. It is concluded that MLH1/3 foci are appropriate markers for the studies of crossovers/chiasmata development and distribution at late meiotic prophase.
Marcon
Edyta
E
Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada.
Moens
Peter
P
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D003434:Crossing Over, Genetic; D005260:Female; D005819:Genetic Markers; D008297:Male; D008540:Meiosis; D051379:Mice; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D019319:Okadaic Acid / Q000494:pharmacology*; D011418:Prophase / Q000235:genetics; D011995:Recombination, Genetic; D016196:S Phase / Q000235:genetics; D013090:Spermatocytes / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013573:Synaptonemal Complex / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D005819:Genetic Markers; C501485:Mlh1 protein, mouse; C492407:Mlh3 protein, mouse; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D019319:Okadaic Acid; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins