title: Interleukin 1alpha single-nucleotide polymorphism associated with systemic sclerosis.
abstract: OBJECTIVE
  In systemic sclerosis (SSc), constitutive expression of the proinflammatory and fibrogenic cytokine interleukin 1alpha (IL-1alpha) by dermal fibroblasts from the affected skin has been observed. We investigated the association of a single-nucleotide polymorphism at position -889 in the IL-1alpha gene in patients with SSc.
  
  
  METHODS
  Genotyping of IL-1alpha-889 polymorphism was performed in 46 patients with SSc and in 150 healthy controls by polymerase chain reaction with sequence-specific primers. All subjects were unrelated Slovak Caucasians.
  
  
  RESULTS
  In SSc patients, carriers of the IL-1alpha-889 T allele were significantly overrepresented in comparison with controls (63.0% vs 42.0%; p = 0.01, OR 2.3, 95% CI 1.2-4.6). The frequency of the IL-1alpha-889 T allele was increased in SSc patients (38.0%) in comparison with controls (25.7%; p = 0.02).
  
  
  CONCLUSIONS
  The IL-1alpha-889 polymorphism, previously shown to predispose to increased IL-1 protein expression, may be involved in susceptibility to SSc.
authors.affiliation: Department of Immunology, Palacky University, Olomouc, Czech Republic.
authors.forename: Beáta
authors.initials: B
authors.lastname: Hutyrová
authors.forename: Jozef
authors.initials: J
authors.lastname: Lukác
authors.forename: Vladimír
authors.initials: V
authors.lastname: Bosák
authors.forename: Milan
authors.initials: M
authors.lastname: Buc
authors.forename: Roland
authors.initials: R
authors.lastname: du Bois
authors.forename: Martin
authors.initials: M
authors.lastname: Petrek
mesh_terms: D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012595:Scleroderma, Systemic / Q000235:genetics*
chemical_list: D007375:Interleukin-1