Cloning and characterization of a Caenorhabditis elegans cDNA encoding a new insulin/IGF-like peptide.
A Caenorhabditis elegans cDNA encoding a new insulin/IGF-like peptide was cloned and examined. The predicted peptide shows significant sequence similarity with the peptide Ceinsulin-1 reported previously and contains a characteristic insertion consisting of three residues in the putative B domain as with the Ceinsulin-1. The gene expression pattern during development is almost identical to that of Ceinsulin-1. The predicted tertiary structure of the peptide is quite similar to that of Ceinsulin-1, and their predicted receptor-recognition surfaces also closely match. These facts suggest that both peptides could recognize the same receptor.
Kawano
Tsuyoshi
T
Department of Biological and Environmental Chemistry, Faculty of Agriculture, Tottori University, Japan. kawano@muses.tottori-u.ac.jp
Takuwa
Kyoko
K
Ishiguro
Masaji
M
Nakajima
Terumi
T
Kimura
Yasuo
Y
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D007328:Insulin / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D007328:Insulin; C424903:ins-1 protein, C elegans; D007334:Insulin-Like Growth Factor I