Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins.
Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel. To help overcome this limitation, we recommend a modification
Rainwater
David L
DL
Department of Genetics, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245-0549, USA. david@darwin.sfbr.org
Moore
Perry H
PH
Gamboa
Israel O
IO
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D005782:Gels / Q000737:chemistry; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification*; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification; D008722:Methods; D012680:Sensitivity and Specificity; D013395:Sucrose
D005782:Gels; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D013395:Sucrose