title: Cytosolic phospholipase A2 type IVA is present in human red cells.
abstract: Phospholipase A(2) type IVA (IVAPLA(2)) is a cytosolic enzyme that on activation selectively releases arachidonic acid (AA) from cell membrane phospholipids. Both AA and lysophospholipid, products of the enzymic reaction, can function as signal transducers in cellular interactions. The enzyme is present in most cells, including polymorphs, eosinophils, and platelets. This study used affinity purification to extract IVAPLA(2) from red cell lysate prepared from leukocyte- and platelet-depleted human blood to overcome the masking effect of hemoglobin on Western blot detection. We show that IVAPLA(2) is present in red cells as a 90-kDa protein.
authors.affiliation: Biochemistry Department, Victoria Infirmary, Glasgow, G42 9TY, United Kingdom. glasgow.pla2group@gvic.scot.nhs.uk
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Macdonald
authors.forename: Rose M
authors.initials: RM
authors.lastname: Boyle
authors.forename: Alastair C A
authors.initials: AC
authors.lastname: Glen
authors.forename: David F
authors.initials: DF
authors.lastname: Horrobin
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity; D003600:Cytosol / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D054513:Group IV Phospholipases A2; D006801:Humans; D008970:Molecular Weight; D010741:Phospholipases A / Q000032:analysis / Q000302:isolation & purification*; D054467:Phospholipases A2; D020298:U937 Cells / Q000201:enzymology
chemical_list: D010741:Phospholipases A; D054513:Group IV Phospholipases A2; C516389:PLA2G4A protein, human; D054467:Phospholipases A2