[Use of molecular markers for differentiation of cultivated strains of oyster and button mushrooms].
Analysis of commercial strains of two edible mushrooms, Pleurotus ostreatus and Agaricus bisporus, using PCR and isozyme electrophoresis techniques allowed us to differentiate groups of genetically similar and distant strains. Among the commercial strains of P. ostreatus, the level of genetic variation was higher suggesting a broader genetic basis employed in breeding of this mushroom. The cultivars and hybrids of, A. bisporus, showed a higher level of homology. The isozyme markers (nonspecific esterase, leucinaminopeptidase, and phosphoglucoisomerase) are recommended for identification of the commercial strains of edible mushrooms.
Shnyreva
A V
AV
Department of Mycology and Algology, Lomonosov Moscow State University, Moscow, 119992 Russia. shnyreva@herba.msu.ru
Belokon'
Iu S
IuS
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M M
MM
D000364:Agaricus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004950:Esterases / Q000235:genetics; D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D007931:Leucyl Aminopeptidase / Q000235:genetics; D020076:Pleurotus / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D017931:DNA Primers; D005819:Genetic Markers; D004950:Esterases; D007931:Leucyl Aminopeptidase; D005956:Glucose-6-Phosphate Isomerase