title: Comparative kinetics of azathioprine and metazathioprine mercaptolysis in presence of physiological thiols.
abstract: Kinetic parameters of mercaptolysis of azathioprine (AZA) and metazathioprine (MAZA) to 6-mercaptopurine in phosphate buffer, pH 7.4, under the influence of physiological thiols (glutathione and cysteine) at 25 degrees, 30 degrees and 37 degrees C were determined and compared. It comes out that the mercaptolysis of MAZA is significantly faster under the influence of both mentioned thiols if compared to that reaction of AZA. Furthermore, the mercaptolysis of MAZA and AZA proceeded significantly faster under the influence of cysteine than on the glutathione heterolysis.
authors.affiliation: Department of Physical Pharmacy and Pharmacokinetics, University of Medical Sciences, 6 Swiecickiego Str., 60 781 Poznań, Poland. mchrzan@am.poznan.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Chrzanowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Hałas
authors.forename: Marta
authors.initials: M
authors.lastname: Kuehn
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Hermann
mesh_terms: D001379:Azathioprine / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D015122:Mercaptopurine / Q000737:chemistry; D011687:Purines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D011687:Purines; D013438:Sulfhydryl Compounds; C481458:methazathioprine; D015122:Mercaptopurine; D003545:Cysteine; D001379:Azathioprine