A new method of measuring the antioxidant activity of polyphenols using cumene hydroperoxide.
BACKGROUND
It is well known that polyphenols lower the risk of chronic diseases such as heart disease and cancer. We describe a new method using cumene hydroperoxide (CHP) to determine the antioxidant activity of polyphenols. CHP is considered to be a model reactive lipid peroxidation product.


METHODS
The method was based on two reactions: the reduction of a known concentration of CHP by polyphenols and the reaction of the remaining CHP with a methylene blue derivative (10-N-methylcarbamoyl-3,7 dimethylamino-10H-phenothiazine) in the presence of haemoglobin. Methylene blue was formed as a result of the two reactions and then measured by absorbance at 675 nm. We named this assay the 'cumene hydroperoxide/haemoglobin.methylene blue (CHP/Hb.MB) method'. We examined 13 polyphenols and nine compounds known to be antioxidants.


RESULTS
Among the 13 polyphenols, milicetin, with the largest number of hydroxyl groups, had the highest antioxidant activity, followed by cyanidin, pelargonidin and quercetin. It is suggested that the increase in the number of hydroxyl groups induced a higher antioxidant activity. Within-run coefficients of variation were 3.6% and 3.5% at the mean antioxidant activity of 50 micromol/L and 151 micromol/L, respectively.


CONCLUSIONS
We conclude that this CHP/Hb.MB assay is capable of measuring the antioxidant activity of polyphenols.
Sugita
Osamu
O
Niigata College of Nursing, Joetsu-City 943-0147, Japan. sugita@niigata-cn.ac.jp
Ishizawa
Nobuhito
N
Matsuto
Takayuki
T
Okada
Masahiko
M
Kayahara
Norihiko
N
D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001555:Benzene Derivatives / Q000032:analysis / Q000737:chemistry*; D002624:Chemistry, Clinical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology; D059808:Polyphenols; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D001555:Benzene Derivatives; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011794:Quercetin; C007164:cumene hydroperoxide