title: Regulation of Ca2+-dependent K+ current by alphavbeta3 integrin engagement in vascular endothelium.
abstract: Interactions between endothelial cells and extracellular matrix proteins are important determinants of endothelial cell signaling. Endothelial adhesion to fibronectin through alpha(v)beta(3) integrins or the engagement and aggregation of luminal alpha(v)beta(3) receptors by vitronectin triggers Ca2+ influx. However, the underlying signaling mechanisms are unknown. The electrophysiological basis of alpha(v)beta(3) integrin-mediated changes in endothelial cell Ca2+ signaling was studied using whole cell patch clamp and microfluorimetry. The resting membrane potential of bovine pulmonary artery endothelial cells averaged -60 +/- 3 mV. In the absence of intracellular Ca2+ buffering, the application of soluble vitronectin (200 microg/ml) resulted in activation of an outwardly rectifying K+ current at holding potentials from -50 to +50 mV. Neither a significant shift in reversal potential (in voltage clamp mode) nor a change in membrane potential (in current clamp mode) occurred in response to vitronectin. Vitronectin-activated current was significantly inhibited by pretreatment with the alpha(v)beta(3) integrin antibody LM609 by exchanging extracellular K+ with Cs+ or by the application of iberiotoxin, a selective inhibitor of large-conductance, Ca2+-activated K+ channels. With intracellular Ca2+ buffered by EGTA in the recording pipette, vitronectin-activated K+ current was abolished. Fura-2 microfluorimetry revealed that vitronectin induced a significant and sustained increase in intracellular Ca2+ concentration, although vitronectin-induced Ca2+ current could not be detected. This is the first report to show that an endothelial cell ion channel is regulated by integrin activation, and this K+ current likely plays a crucial role in maintaining membrane potential and a Ca2+ driving force during engagement and activation of endothelial cell alpha(v)beta(3) integrin.
authors.affiliation: Department of Medical Physiology, Texas A&M University System Health Science Center, College Station, TX 77843-1114, USA.
authors.forename: Junya
authors.initials: J
authors.lastname: Kawasaki
authors.forename: George E
authors.initials: GE
authors.lastname: Davis
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Davis
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D002586:Cesium / Q000737:chemistry; D004533:Egtazic Acid / Q000737:chemistry; D004594:Electrophysiology; D004730:Endothelium, Vascular / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D016257:Fura-2 / Q000494:pharmacology; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D007477:Ions / Q000737:chemistry; D008856:Microscopy, Fluorescence; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology; D011188:Potassium / Q000737:chemistry*; D011485:Protein Binding; D015398:Signal Transduction; D013997:Time Factors; D019096:Vitronectin / Q000378:metabolism
chemical_list: D005353:Fibronectins; D039302:Integrin alphaVbeta3; D007477:Ions; D010455:Peptides; D019096:Vitronectin; D002586:Cesium; D004533:Egtazic Acid; C064719:iberiotoxin; D011188:Potassium; D002118:Calcium; D016257:Fura-2