The lipocalin 24p3, which is an essential molecule in IL-3 withdrawal-induced apoptosis, is not involved in the G-CSF withdrawal-induced apoptosis.
Many hematopoietic cells undergo apoptosis when deprived of specific cytokines. Lipocalin 24p3, reported to be induced in hematopoietic cells by interleukin 3 (IL-3) depletion, induces hematopoietic cell apoptosis despite the presence of IL-3. As granulocyte colony stimulating factor (G-CSF) depletion also induces the apoptosis of G-CSF-dependent cell line cells, we examined the effect of 24p3 on the apoptotic function induced by G-CSF depletion. 24p3 was induced by the depletion of IL-3, but not G-CSF, in cytokine-dependent cell lines. Although 24p3 suppressed growth induced by IL-3, it did not influence G-CSF-dependent cell growth. These observations show that 24p3 is not involved in the G-CSF withdrawal-induced apoptosis, although it is essential in IL-3 withdrawal-induced apoptosis.
Kamezaki
Kenjirou
K
The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Shimoda
Kazuya
K
Numata
Akihiko
A
Aoki
Kenichi
K
Kato
Kouji
K
Takase
Ken
K
Nakajima
Hideaki
H
Ihara
Kenji
K
Haro
Takashi
T
Ishikawa
Fumihiko
F
Imamura
Rie
R
Miyamoto
Toshihiro
T
Nagafuji
Koji
K
Gondo
Hisashi
H
Hara
Toshiro
T
Harada
Mine
M
D000209:Acute-Phase Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D017077:Culture Media, Conditioned; D015870:Gene Expression; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism*; D000071068:Lipocalin-2; D054834:Lipocalins; D051379:Mice; D009504:Neutrophils / Q000166:cytology; D015513:Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins
D000209:Acute-Phase Proteins; D017077:Culture Media, Conditioned; D007377:Interleukin-3; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; C071332:Lcn2 protein, mouse; D016179:Granulocyte Colony-Stimulating Factor