Fourier transform infrared microscopic imaging: effects of estrogen and estrogen deficiency on fracture healing in rat femurs.
Infrared spectroscopic imaging with 6-10 microm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.
Ouyang
Hao
H
Rutgers University, Department of Chemistry, Newark, New Jersey 07102, USA.
Sherman
Pamela J
PJ
Paschalis
Eleftherios P
EP
Boskey
Adele L
AL
Mendelsohn
Richard
R
D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D004967:Estrogens / Q000008:administration & dosage* / Q000172:deficiency*; D005260:Female; D005264:Femoral Fractures / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology*; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D004967:Estrogens