[Evaluation of interaction between drugs and ordered phospholipid membrane by immobilized artificial membrane chromatography].
OBJECTIVE
To investigate the interaction between drugs and ordered phospholipid membrane using immobilized artificial membrane chromatography (IAMC).


METHODS
IAMC was used to determine the interaction drugs with phospholipid membrane, expressed as membrane affinity (lg kIAM). An n-octanol/buffer system was also employed as the reference hydrophobicity (lg Do/w,7.4).


RESULTS
Within the range of used acetonitrile percentages (phi) 0-30% in mobile phase, retention index (lg kIAM) showed excellent correlation with phi. Intercepts of fitted straight lines between lg kIAM and phi were comparable but slopes were much different for the three organic modifiers (acetonitrile, ethanol and methanol). Effects by adding CH2 substituent on lipophilicity difference (delta lg kIAM and delta lg Do/w,7.4) were similar for p-hydroxyl benzoic methyl ester to butyl ester, whereas different for p-hydroxylbenzoic acid to methyl ester.


CONCLUSIONS
IAMC system is a convenient, efficient and rapid tool for determining membrane interaction.
Sun
Jin
J
Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. sunjin66@21cn.com
Cheng
Gang
G
He
Zhong-gui
ZG
Wang
Shu-jun
SJ
D008567:Membranes, Artificial; D000442:Octanols / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*
D008567:Membranes, Artificial; D000442:Octanols; D004364:Pharmaceutical Preparations; D010743:Phospholipids