Laser desorption and imaging of proteins from ice via UV femtosecond laser pulses.
We have employed 200-fs, 400-nm laser pulses to desorb intact protein molecular ions directly from a frozen aqueous matrix. The resulting spectra obtained using a variety of proteins varying in molecular weight from 1060 (bradykinin) to 5778 Da (insulin) are compatible with those obtained with traditional matrix-assisted laser desorption/ionization experiments. High-quality spectra could be generated using a fluence of 4.0-9.0 J/cm2 to desorb proteins from an aqueous solution frozen onto metal substrates with a sensitivity in the femtomole range. Although the mechanism behind this effect is still not clear, we speculate that it involves explosive boiling of the ice layer due to rapid heating of the substrate. Imaging experiments conducted on the ice layer suggest that the yield of protein is approximately independent of the film thickness and is very reproducible from shot to shot. The results are particularly significant since they open the possibility of examining a range of biomaterials directly from the in vivo aqueous environment.
Berry
Jamal I
JI
Department of Chemistry and Materials Research Institute, Pennsylvania State University, University Park, Pennsylvania 16801, USA.
Sun
Shixin
S
Dou
Yusheng
Y
Wucher
Andreas
A
Winograd
Nicholas
N
D007053:Ice / Q000032:analysis*; D036103:Nanotechnology / Q000379:methods; D011506:Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
D007053:Ice; D011506:Proteins