Isolation of an ozone-sensitive and jasmonate-semi-insensitive Arabidopsis mutant (oji1).
A novel ozone-sensitive mutant was isolated from Arabidopsis T-DNA tagging lines. This mutant revealed severe foliar injury and higher ethylene emission than the wild type under ozone exposure. The ozone-induced injury and ethylene emission were suppressed by pretreatment with aminoethoxyvinyl glycine, an inhibitor of ethylene biosynthesis, both in this mutant and wild-type plants. Pretreatment with methyl-jasmonate (MeJA) at 10 micro M, however, suppressed the ozone-induced ethylene emission and foliar injury only in the wild-type plants. This mutant was less sensitive to jasmonate than the wild type, estimated by the MeJA-induced inhibition of root elongation and ozone-induced expression of AtVSP1, a jasmonate-inducible gene. Thus, this mutant was named oji1 (ozone-sensitive and jasmonate-insensitive 1). These results suggest that the ozone sensitivity of oji1 is caused by the increase in ozone-induced emission of ethylene as a result of low sensitivity to jasmonate, which plays defensive roles under stress conditions.
Kanna
Machi
M
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501 Japan.
Tamaoki
Masanori
M
Kubo
Akihiro
A
Nakajima
Nobuyoshi
N
Rakwal
Randeep
R
Agrawal
Ganesh Kumar
GK
Tamogami
Shigeru
S
Ioki
Motohide
M
Ogawa
Daisuke
D
Saji
Hikaru
H
Aono
Mitsuko
M
D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D003517:Cyclopentanes / Q000494:pharmacology*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis; D009154:Mutation; D054883:Oxylipins; D010126:Ozone / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010126:Ozone; C072239:methyl jasmonate; C036216:ethylene; C441479:VSP1 protein, Arabidopsis; D010450:Endopeptidases