Fine mapping of the circling (cir) gene on the distal portion of mouse chromosome 9.
Circling mice manifest profound deafness, head-tossing, and bi-directional circling behavior, which they inherit in autosomal recessive manner. Histologic examination of the inner ear reveals abnormalities of the region around the organ of Corti, spiral ganglion neurons, and outer hair cells. A genetic linkage map was constructed for an intraspecific backcross between cir and C57BL/6J mice. The cir gene was mapped to a region between D9Mit116/D9Mit15 and D9Mit38 on mouse chromosome (Chr) 9. Estimated distances between cir and D9Mit116, and between cir and D9Mit38 were 0.70 +/- 0.40 and 0.23 +/- 0.23 cM, respectively. Order of the markers was defined as follows: centromere - D9Mit182 - D9Mit51/D9Mit79/D9Mit310 - D9Mit212/D184 - D9Mit116/D9Mit15 - cir - D9Mit38 - D9Mit20 - D9Mit243 - D9Mit16 - D9Mit55/D9Mit125 - D9Mit281. On the basis of genetic mapping, we constructed a yeast artificial chromosome (YAC) contig across the cir region. The cir gene is located between the lactotransferrin (ltf) and microtubule-associated protein (map4) genes. The distal portion of mouse Chr 9 encompassing the cir region is homologous with human chromosome 3p21, which contains the Deafness, form B: Autosomal Recessive Deafness (DFNB6) locus. Therefore, the circling mouse is a potential animal model for DFNB6 deafness in humans.
Cho
Kyoung In
KI
Catholic Research Institutes of Medical Science, Catholic Medical College, 505 Banpo-Dong, Seocho-Ku, Seoul 137-701, Korea.
Lee
Jeong Woong
JW
Kim
Kil Soo
KS
Lee
Eun Ju
EJ
Suh
Jun-Gyo
JG
Lee
Ho-Joon
HJ
Kim
Hyun Taek
HT
Hong
Sung Hwa
SH
Chung
Won Ho
WH
Chang
Kyu Tae
KT
Hyun
Byung Hwa
BH
Oh
Yang-Seok
YS
Ryoo
Zae Young
ZY
D000818:Animals; D001522:Behavior, Animal; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002893:Chromosomes, Human, Pair 3; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping; D003433:Crosses, Genetic; D003638:Deafness / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005808:Genes, Recessive; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008817:Mice, Mutant Strains / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics