A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.
Tse
Winston C
WC
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Boger
Dale L
DL
D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D009429:Netropsin / Q000737:chemistry / Q000378:metabolism; D009757:Nylons / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D009757:Nylons; D009838:Oligodeoxyribonucleotides; D013844:Thiazoles; D009429:Netropsin; D004247:DNA