title: Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification.
abstract: We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.
authors.affiliation: Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors.forename: Marian
authors.initials: M
authors.lastname: Iwamoto
authors.forename: Debabrata
authors.initials: D
authors.lastname: Banerjee
authors.forename: Lata G
authors.initials: LG
authors.lastname: Menon
authors.forename: Anna
authors.initials: A
authors.lastname: Jurkiewicz
authors.forename: Pulivarthi H
authors.initials: PH
authors.lastname: Rao
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Kemeny
authors.forename: Yuman
authors.initials: Y
authors.lastname: Fong
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Jhanwar
authors.forename: Richard
authors.initials: R
authors.lastname: Gorlick
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Bertino
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000556:secondary*; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D005784:Gene Amplification; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000556:secondary*; D008175:Lung Neoplasms / Q000235:genetics* / Q000556:secondary*; D016133:Polymerase Chain Reaction; D013940:Thymidylate Synthase / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D014157:Transcription Factors; D013940:Thymidylate Synthase