Comparison of gene expression between left atria and left ventricles from non-diseased humans.
We examine the reliability and accuracy of gene array technology in analyzing differences in gene expression between human non-diseased left atrium and left ventricle. We have used cDNA gene arrays and validated those data by carefully designed quantitative real-time polymerase chain reaction (PCR). We have identified pitfalls using cDNA gene array technology based on comparisons with other gene array studies and with changes reported for the levels of expression of the genes corresponding to these cDNAs. The high error rate reported here underscores the cautionary comments reported by others in this field.
Tsubakihara
Masako
M
Institute for Biomedical Research, University of Sydney, Sydney, Australia. masako@anatomy.usyd.edu.au
Williams
Neal K
NK
Keogh
Anne
A
dos Remedios
Cristobal G
CG
D016275:Atrial Function / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009626:Terminology as Topic; D016276:Ventricular Function / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D012333:RNA, Messenger