title: Ex vivo selection of recipient-type alloantigen-specific CD4(+)CD25(+) immunoregulatory T cells for the control of graft-versus-host disease after allogeneic hematopoietic stem-cell transplantation.
abstract: Allogeneic hematopoietic stem-cell transplantation (HSCT) is the treatment of choice for many malignant and nonmalignant hematologic disorders. Donor T cells present in the hematopoietic stem-cell transplant improve engraftment and immune reconstitution and contribute to the graft-versus-leukemia effect, but are also responsible for the life-threatening graft-versus-host disease (GVHD). CD4(+)CD25(+) immunoregulatory T cells, which play a pivotal role in preventing organ-specific diseases, can also modulate GVHD if administered in equal numbers of T cells at the time of grafting. In this article, the authors describe a procedure of ex vivo selection and expansion of regulatory T cells specific for recipient-type alloantigens. These expanded regulatory T cells controlled GVHD. Their therapeutic use in HSCT should allow specific suppression of the activation of donor alloreactive T cells involved in GVHD while preserving the beneficial effects of other T cells.
authors.affiliation: Biologie et Thérapeutique des Pathologies Immunitaires, Hôpital Pitié-Salpêtrière, Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Trenado
authors.forename: Sylvain
authors.initials: S
authors.lastname: Fisson
authors.forename: Eric
authors.initials: E
authors.lastname: Braunberger
authors.forename: David
authors.initials: D
authors.lastname: Klatzmann
authors.forename: Benoît L
authors.initials: BL
authors.lastname: Salomon
authors.forename: José L
authors.initials: JL
authors.lastname: Cohen
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation*; D006086:Graft vs Host Disease / Q000209:etiology* / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D014184:Transplantation, Homologous / Q000009:adverse effects*
chemical_list: D007519:Isoantigens; D015375:Receptors, Interleukin-2