Inhibitory effects of hyssop (Hyssopus officinalis) extracts on intestinal alpha-glucosidase activity and postprandial hyperglycemia.
It has been known that Hyssopus officinalis (hyssop) is a herb that grows in the wild and is a source of natural antioxidants. We previously reported that a-glucosidase inhibitors, (2S, 3S)1-O-beta-D-6'-O-cinnamoylglucopyranosyl-3-(3", 5"-dimethoxy-4"-hydroxyphenyl)-1,2,3-propanetriol and (2S, 3S)1-O-beta-D-glucopranosyl-3-(3", 5"-dimethoxy-4"-hydroxyphenyl)-1,2,3-propanetriol, from the dry leaves of hyssop, were isolated. This study examined the alpha-glucosidase inhibitory effects of hyssop extracts on intestinal carbohydrate absorption in rat everted gut sac and carbohydrate-loaded hyperglycemia in mice. In the everted gut sac experiment, 10 mM sucrose- and 5 mM maltose-treated increases in glucose concentration in the serosal compartment were inhibited in the presence of 0.5 and 1.0 mg/ mL hyssop extracts, although a 10 mM glucose-induced increase in serosal glucose was not inhibited by the extracts. Additionally, hyperglycemia in sucrose- and maltose-loaded mice was significantly suppressed at an early stage, within 30 to 60 min by oral pre-administration of 300 and 100 mg/kg hyssop extracts, respectively. These findings suggest that hyssop extracts inhibited the digestion of complex carbohydrates, but not that of absorbable monosaccharide, and might be a useful supplemental food for hyperglycemia.
Miyazaki
Hiroyuki
H
Northern Advancement Center for Science and Technology, Sapporo 001-0021, Japan. hmiya@rakuno.ac.jp
Matsuura
Hideyuki
H
Yanagiya
Chikako
C
Mizutani
Junya
J
Tsuji
Masayoshi
M
Ishihara
Chiaki
C
D000818:Animals; D050260:Carbohydrate Metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005502:Food; D005947:Glucose / Q000032:analysis / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D006943:Hyperglycemia / Q000517:prevention & control*; D007408:Intestinal Absorption / Q000187:drug effects; D007422:Intestines / Q000201:enzymology*; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008320:Maltose / Q000008:administration & dosage; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000008:administration & dosage; D000520:alpha-Glucosidases / Q000378:metabolism
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D013395:Sucrose; D008320:Maltose; D000520:alpha-Glucosidases; D005947:Glucose