Lack of action potential-prolonging effect of terfenadine on rabbit myocardial tissue preparations.
The effects of terfenadine, an antiallergic drug also known for its QT-prolonging and arrhythmogenic activities, on the action potential of isolated myocardial tissue preparations from rabbits were examined with microelectrode techniques. In the Purkinje fibers and atrium, terfenadine concentration dependently decreased the maximum rate of rise (+.V(max)) without affecting other action potential parameters. In the ventricle, terfenadine had little effect on action potential configuration. In the sinoatrial node, terfenadine 20 microM prolonged cycle length mainly through inhibition of +.V(max). Terfenadine 1 microM completely inhibited the human ether a go-go-related gene (HERG) channel current expressed in HEK293 cells in the same experimental solution as in microelectrode experiments. The lack of terfenadine effect on the action potential duration suggests that there are drugs for which the HERG channel inhibitory action underlying in vivo QT prolongation cannot be evaluated based on their action potential-prolonging activity in isolated myocardial tissue preparations.
Masumiya
Haruko
H
Department of Pharmacology, Toho University School of Pharmaceutical Sciences, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Saito
Mariko
M
Ito
Mie
M
Matsuda
Tomoyuki
T
Noguchi
Kazuo
K
Iida-Tanaka
Naoko
N
Tanaka
Hikaru
H
Shigenobu
Koki
K
D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004562:Electrocardiography / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006352:Heart Ventricles / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008839:Microelectrodes; D011690:Purkinje Fibers / Q000187:drug effects; D011817:Rabbits; D016593:Terfenadine / Q000494:pharmacology*
D018926:Anti-Allergic Agents; D018076:DNA, Complementary; D016593:Terfenadine