[A simple method to detect disaccharides deficiency].
A simple method, easy to perform during an endoscopic procedure, fast and inexpensive, that allows detecting deficiencies in lactase, sucrase or maltase activities is presented. Briefly, method consists in placing a duodenal biopsy sample in an adequate vial containing lactose, sucrose or maltose solution during a few minutes, and then, adding a few drops of a glucose reactive from commercial origin. Presence of any enzymatic activity is demonstrated when released glucose from any of the disaccharides chosen reacts with the second reactive, turning solution to a red colour. Its utility is discussed and compared with other diagnostic methods.
Laguens
M
M
Centro Gastroenterológico Platense, Calle 3 No 1095 (1900), La Plata, Argentina.
Collura
J
J
Marrone
R
R
Diogurdi
E
E
Lequerica
J
J
Camaño
M
M
D004796:Clinical Enzyme Tests / Q000379:methods*; D003124:Colorimetry; D004186:Disaccharidases / Q000172:deficiency*; D004384:Duodenoscopy; D004386:Duodenum / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D007785:Lactose / Q000172:deficiency; D008297:Male; D008320:Maltose / Q000172:deficiency; D013393:Sucrase / Q000172:deficiency
D008320:Maltose; D004186:Disaccharidases; D013393:Sucrase; D007785:Lactose