Performance and use of a ribonucleotide reductase herpes simplex virus type-specific serological assay.
In response to the increasingly evident need for herpes simplex virus (HSV) serotype-specific serologic assays that rely on proteins other than glycoprotein-G (gG), we developed a rapid serologic assay that is based on type-specific epitopes within the large subunit of HSV ribonucleotide reductase (R1). The assay (Au-2 enzyme-linked immunosorbent assay [ELISA]) uses an HSV type 2 (HSV-2) R1 peptide antigen. It provides a reliable method for detecting serotype-specific antibody to a protein other than gG-2. The Au-2 ELISA has high sensitivity and specificity as determined by direct comparison to Western blotting, a widely accepted "gold standard," and to ELISA with an HSV-1 R1 peptide (Au-1). The use of the Au-2 ELISA in conjunction with the gG-2-based assays will improve the sensitivity and specificity of serologic diagnosis and patient management.
Wales
S Q
SQ
AuRx, Inc., Glen Burnie, Maryland 21061, USA.
Smith
C C
CC
Wachsman
M
M
Calton
G
G
Aurelian
L
L
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006558:Herpes Genitalis / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D006561:Herpes Simplex / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000201:enzymology* / Q000276:immunology*; D006801:Humans; D012264:Ribonucleotide Reductases / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*
D012264:Ribonucleotide Reductases