A distinctive ribonuclease from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum.
A ribonuclease with an N-terminal sequence distinct from other mushroom ribonucleases was isolated from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. The ribonuclease was adsorbed on DEAE-cellulose and Q-Sepharose, and unadsorbed on CM-Sepharose. It possessed a molecular mass of 42 kDa as judged by gel filtration by fast protein liquid chromatography on Superdex 75 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its molecular mass was similar to that of straw mushroom ribonuclease but much higher compared with those of other mushroom ribonucleases. The ribonuclease was unique among mushroom ribonucleases in that it exhibited the highest potency toward poly(U), followed by poly(A). Its activity toward poly(G) and poly(C) was about one-half of that toward poly(A) and one-quarter of that toward poly(U). A pH of 4.0 and a temperature of 60 degrees C were required for optimal activity of the enzyme. The optimum pH was low compared with those reported for other mushroom ribonucleases.
Wang
H X
HX
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
Ng
T B
TB
Chiu
S W
SW
D000363:Agaricales / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D003636:DEAE-Cellulose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts; D017434:Protein Structure, Tertiary; D020940:Reishi / Q000201:enzymology*; D012260:Ribonucleases / Q000737:chemistry*; D012265:Ribonucleotides / Q000737:chemistry; D012685:Sepharose / Q000494:pharmacology; D013045:Species Specificity; D013696:Temperature
D005656:Fungal Proteins; D010936:Plant Extracts; D012265:Ribonucleotides; D012685:Sepharose; D003636:DEAE-Cellulose; D012260:Ribonucleases