title: Sulphate metabolism of selenate-resistant Schizosaccharomyces pombe mutants.
abstract: Selenate-resistant mutants were obtained from several strains of Schizosaccharomyces pombe. The obtained mutants all belonged to the same genetic complementation group. They were low in sulphate uptake activity and in ATP sulphurylase activity. They grew on medium containing sulphite, thiosulphate, cysteine or glutathione but not methionine as the sole source of sulphur. From these results, the mutants were concluded to carry mutations in the ATP sulphurylase gene. Inability of the mutants to utilize methionine as a sulphur source is rationalized by the absence of the reverse transsulphurylation pathway in this organism; wild type strains must utilize methionine as a sulphur source after it is degraded to give rise to sulphate.
authors.affiliation: Department of Microbiology and Biotechnology, Faculty of Food Science, Budapest Corvinus University, H-1118 Budapest, Somlói ut 14-16, Hungary.
authors.forename: Luca
authors.initials: L
authors.lastname: Bánszky
authors.forename: Tibor
authors.initials: T
authors.lastname: Simonics
authors.forename: Anna
authors.initials: A
authors.lastname: Maráz
mesh_terms: D025141:Drug Resistance, Fungal; D009154:Mutation; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D018036:Selenium Compounds / Q000494:pharmacology; D013430:Sulfate Adenylyltransferase / Q000235:genetics / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D018036:Selenium Compounds; D013431:Sulfates; D013430:Sulfate Adenylyltransferase