Chlorate: a reversible inhibitor of proteoglycan sulphation in Chlamydia trachomatis-infected cells.
Sulphated glycosaminoglycans, such as heparan sulphate, have been shown to be essential for the infectivity of many organisms. The aims of this study were to verify the role of sulphated glycosaminoglycans in chlamydial infection and to investigate whether they are present on chlamydia or chlamydial host cells. The effect of undersulphation of host cells and chlamydial elementary bodies was examined using sodium chlorate. Also studied was whether any inhibitory effect was reversible. The results strongly suggest that Chlamydia trachomatis does not produce heparan sulphate and that heparan sulphate of the host cell is necessary and sufficient to mediate chlamydial infection. The essential role played by the sulphate constituents of the host-cell glycosaminoglycan in the infectivity of LGV serovars, and to a lesser extent of serovar E, was also confirmed.
Fadel
Sanaa
S
Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield, S10 2RX, UK.
Eley
Adrian
A
Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield, S10 2RX, UK.
D000818:Animals; D002460:Cell Line; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification / Q000187:drug effects* / Q000472:pathogenicity* / Q000648:ultrastructure; D002704:Chlorates / Q000494:pharmacology*; D006367:HeLa Cells; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011509:Proteoglycans / Q000378:metabolism; D012703:Serotyping; D013455:Sulfur / Q000378:metabolism
D002704:Chlorates; D006497:Heparan Sulfate; D011509:Proteoglycans; D013455:Sulfur; C032706:sodium chlorate