[Crosslinking bovine pericardial bioprosthetic heart valves by phthalocyanine sensitized photooxidation reaction].
OBJECTIVE
To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.


METHODS
Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.


RESULTS
Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.


CONCLUSIONS
Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.
Lin
Lin
L
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Tang
Ying-wu
YW
Liang
Qiang
Q
Zhou
Jian-ye
JY
Cui
Jing-wei
JW
Hu
Sheng-shou
SS
Zhang
Fu-shi
FS
D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006350:Heart Valve Prosthesis; D007211:Indoles; D054833:Isoindoles; D010496:Pericardium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D003432:Cross-Linking Reagents; D007211:Indoles; D054833:Isoindoles; C013647:phthalocyanine