In vivo substrate specificity of periplasmic disulfide oxidoreductases.
In Escherichia coli, a family of periplasmic disulfide oxidoreductases catalyzes correct disulfide bond formation in periplasmic and secreted proteins. Despite the importance of native disulfide bonds in the folding and function of many proteins, a systematic investigation of the in vivo substrates of E. coli periplasmic disulfide oxidoreductases, including the well characterized oxidase DsbA, has not yet been performed. We combined a modified osmotic shock periplasmic extract and two-dimensional gel electrophoresis to identify substrates of the periplasmic oxidoreductases DsbA, DsbC, and DsbG. We found 10 cysteine-containing periplasmic proteins that are substrates of the disulfide oxidase DsbA, including PhoA and FlgI, previously established DsbA substrates. This technique did not detect any in vivo substrates of DsbG, but did identify two substrates of DsbC, RNase I and MepA. We confirmed that RNase I is a substrate of DsbC both in vivo and in vitro. This is the first time that DsbC has been shown to affect the in vivo function of a native E. coli protein, and the results strongly suggest that DsbC acts as a disulfide isomerase in vivo. We also demonstrate that DsbC, but not DsbG, is critical for the in vivo activity of RNase I, indicating that DsbC and DsbG do not function identically in vivo. The absence of substrates for DsbG suggests either that the in vivo substrate specificity of DsbG is more limited than that of DsbC or that DsbG is not active under the growth conditions tested. Our work represents one of the first times the in vivo substrate specificity of a folding catalyst system has been systematically investigated. Because our methodology is based on the simple assumption that the absence of a folding catalyst should cause its substrates to be present at decreased steady-state levels, this technique should be useful in analyzing the substrate specificity of any folding catalyst or chaperone for which mutations are available.
Hiniker
Annie
A
Program in Cellular and Molecular Biology, University of Michigan, 830 N. University, Ann Arbor, MI 48109-1048, USA.
Bardwell
James C A
JC
D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry; D013058:Mass Spectrometry; D010088:Oxidoreductases / Q000737:chemistry; D019897:Periplasm / Q000201:enzymology* / Q000378:metabolism; D033903:Periplasmic Proteins / Q000737:chemistry; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000737:chemistry*; D017510:Protein Folding; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D013379:Substrate Specificity; D013997:Time Factors; D014238:Trichloroacetic Acid / Q000737:chemistry
D004220:Disulfides; D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D014238:Trichloroacetic Acid; D010088:Oxidoreductases; C109831:DsbG protein, E coli; D012259:Ribonuclease, Pancreatic; D019704:Protein Disulfide-Isomerases; D003545:Cysteine