[Molecular chaperone GRP75 reprove cells from injury caused by glucose deprivation].
Glucose-regulated proteins 75(grp75) is a member of hsp70 family. The expression of grp75 is upregulated during glucose starvation (such as ischemia). To evaluate grp75 function, CHL cells were cultured with glucose-free media for 20 h (A) and glucose-free media for 12 h + glucose-containing media for 8 h (ischemia reperfusion) (B). A constructed rat grp75 cDNA expression vector (pcDNA/grp75) was transfected into CHL cells and a cell strain that stably overexpressed grp75 was obtained. The transfected cells and untransfected cells(control group) were cultured with A or B. By MTT, LDH leakage measurement and flow cytometry analysis, growth rate of untransfected cells in B is significantly lower than that in glucose-containing media for 20 h (C) (p < 0.05) and A (p < 0.05). Growth rate of transfected cells is apparently higher than that of control group in B (p < 0.01). LDH liberation percentage of untransfected cells in B is obviously higher than that in C(p < 0.01) and it is not different from A(p > 0.05). LDH liberation percentage of transfected cells is apparently lower than that of control group in B(p < 0.01). Apoptosis of transfected cells is obviously lower by flow cytometry analysis. These results provide evidence for the cytoprotective function of grp75 during glucose starving and ischemia reperfusion.
Gao
Cui Xiang
CX
College of Life Science, Nanjing Normal University, Nanjing 210097.
Zhang
Shuang Quan
SQ
Yin
Zhimin
Z
Liu
Wen
W
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D006224:Cricetinae; D019610:Cytoprotection; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D005947:Glucose / Q000172:deficiency*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007511:Ischemia / Q000150:complications; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism; D014162:Transfection
D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C018551:glucose-regulated proteins; D007770:L-Lactate Dehydrogenase; D005947:Glucose