Dynamic changes of capillarization and peri-sinusoid fibrosis in alcoholic liver diseases.
OBJECTIVE
To investigate the dynamic changes of capillarization and peri-sinusoid fibrosis in an alcoholic liver disease model induced by a new method.


METHODS
Male SD rats were randomly divided into 6 groups, namely normal, 4 d, 2 w, 4 w, 9 w and 11 w groups. The animals were fed with a mixture of alcohol for designated days and then decollated, and their livers were harvested to examine the pathological changes of hepatocytes, hepatic stellate cells, sinusoidal endothelial cells, sinusoid, peri-sinusoid. The generation of three kinds of extra cellular matrix was also observed.


RESULTS
The injury of hepatocytes became severer as modeling going on. Under electronic microscope, fatty vesicles and swollen mitochondria in hepatocytes, activated hepatic stellate cells with fibrils could been seen near or around it. Fenestrae of sinusoidal endothelial cells were decreased or disappeared, sinusoidal basement was formed. Under light microscopy typical peri-sinusoid fibrosis, gridding-like fibrosis, broaden portal areas, hepatocyte's fatty and balloon denaturation, iron sediment, dot necrosis, congregated lymphatic cells and leukocytes were observed. Type I collagen showed an increasing trend as modeling going on, slightly recovered when modeling stopped for 2 weeks. Meanwhile, type IV collagen decreased rapidly when modeling began and recovered after modeling stopped for 2 weeks. Laminin increased as soon as modeling began and did not recover when modeling stopped for 2 weeks.


CONCLUSIONS
The pathological changes of the model were similar to that of human ALD, but mild in degree. It had typical peri-sinusoid fibrosis, however, capillarization seemed to be instable. It may be related with the reduction of type IV collagen in the basement of sinusoid during modeling.
Xu
Guang-Fu
GF
Digestive Department of the Affiliated Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China. guangfuxu@hotmail.com
Wang
Xin-Yue
XY
Ge
Gui-Ling
GL
Li
Peng-Tao
PT
Jia
Xu
X
Tian
De-Lu
DL
Jiang
Liang-Duo
LD
Yang
Jin-Xiang
JX
D000818:Animals; D002196:Capillaries / Q000473:pathology; D024042:Collagen Type I / Q000032:analysis; D024141:Collagen Type IV / Q000032:analysis; D004195:Disease Models, Animal; D005355:Fibrosis; D022781:Hepatocytes / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D007150:Immunohistochemistry; D008102:Liver Circulation; D008108:Liver Diseases, Alcoholic / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D024042:Collagen Type I; D024141:Collagen Type IV