Genetic diversity and population structure of Mycobacterium tuberculosis in Casablanca, a Moroccan city with high incidence of tuberculosis.
Although lower-resource countries have by far the highest burden of tuberculosis, knowledge of Mycobacterium tuberculosis population structure and genetic diversity in these regions remains almost nonexistent. In this paper, 150 Moroccan M. tuberculosis isolates circulating in Casablanca were genotyped by random amplified polymorphic DNA analysis using 10 different primers and by mycobacterial interspersed repetitive units-variable number of tandem repeats typing at 12 loci. The population genetic tests revealed a basically clonal structure for this population, without excluding rare genetic exchanges. Genetic analysis also showed a notable genetic polymorphism for the species M. tuberculosis, a weak cluster individualization, and an unexpected genetic diversity for a population in such a high-incidence community. Phylogenetic analyses of this Moroccan sample also supported that these isolates are genetically heterogeneous.
Tazi
Loubna
L
Génétique des Maladies Infectieuses, UMR CNRS-IRD 9926, IRD, 34394 Montpellier Cedex 5, France. ltazi@uci.edu
El Baghdadi
Jamila
J
Lesjean
Sarah
S
Locht
Camille
C
Supply
Philip
P
Tibayrenc
Michel
M
Bañuls
Anne-Laure
AL
D014644:Genetic Variation; D006801:Humans; D015994:Incidence; D009018:Morocco / Q000453:epidemiology; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology