title: On the pathogenicity of attenuated Schistosoma mansoni cercariae released from metabolically disturbed Biomphalaria alexandrina.
abstract: Biomphalaria alexandrina were treated with sublethal concentrations (LC10) of dry powdered leaves Solanum nigrum or whole dry Ambrosia maritima. The two plants affected the development of schistosome parasite within snails by disturbing the glycolytic flux, the most important metabolic pathway for schistosome-infected snails. Attenuated cercariae released from treated snails were used to infect male albino mice to evaluate their pathogenicity compared to control cercariae shed from untreated snails. The mean number of worms established declined from about 70 +/- 17.6 worms/mouse infected with control cercariae to 23.4 +/- 20.33 and 14.18 +/- 10.06 worms/mouse infected with S. nigrum and A. maritima-attenuated cercariae respectively. Most males and females detected in these animals measured 0.4-0.6 and 0.6-1.2 mm respectively compared to 1.2-1.4 and 1.4-1.7 mm in males and females released from mice infected with normal cercariae. Egg count in the liver and intestine of mice infected with attenuated cercariae was remarkably lower showing reduced fecundity of worms developed from attenuated cercariae. Number and size of granulomatous reactions showed remarkable reduction in attenuated cercariae-infected mice. Biochemical analyses for pathogenicity achieved with attenuated cercariae showed that while serum aspartate and alanine aminotransferases (AST &ALT) were more or less similar, depleted glycogen and elevated lipid peroxides were normalized when compared to those infected with normal cercariae.
authors.affiliation: Department of Medicinal Chemistry, National Research Centre, Dokki, Cairo, Egypt. Aelansarynrc50@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: El-Ansary
authors.forename: A M
authors.initials: AM
authors.lastname: Mohamed
authors.forename: S S
authors.initials: SS
authors.lastname: Mahmoud
authors.forename: S
authors.initials: S
authors.lastname: El-Bardicy
mesh_terms: D031164:Ambrosia / Q000737:chemistry*; D000818:Animals; D001700:Biomphalaria / Q000378:metabolism* / Q000469:parasitology; D005260:Female; D005298:Fertility / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D006790:Host-Parasite Interactions / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008975:Molluscacides / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000472:pathogenicity*; D012555:Schistosomiasis mansoni / Q000378:metabolism / Q000469:parasitology* / Q000517:prevention & control; D032324:Solanum nigrum / Q000737:chemistry*
chemical_list: D008975:Molluscacides; D010936:Plant Extracts