Determination of phenolic constituents of biological interest in red wine by capillary electrophoresis with electrochemical detection.
A simultaneous determination of trans-resveratrol, (-)-epicatechin, and (+)-catechin in red wine by capillary electrophoresis with electrochemical detection (CE-ED) is reported. The effects of the potential of the working electrode, pH and concentration of running buffer, separation voltage, and injection time on CE-ED were investigated. Under the optimum conditions, the analytes could be separated in a 100 mmol/L borate buffer (pH 9.2) within 20 min. A 300 microm diameter carbon disk electrode has a good response at +0.85 V (vs SCE) for all analytes. The response was linear over 3 orders of magnitude with detection limit (S/N = 3) ranging from 2 x 10(-7) to 5 x 10(-7) g/mL for all analytes. This method has been used for the determination of these analytes in red wine without enrichment, and the assay result was satisfactory.
Peng
Youyuan
Y
Department of Chemistry, East China Normal University, North Zhongshan Road, Shanghai 200062, People's Republic of China.
Chu
Qingcui
Q
Liu
Fanghua
F
Ye
Jiannong
J
D002392:Catechin / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis; D014920:Wine / Q000032:analysis*
D010636:Phenols; D013267:Stilbenes; D002392:Catechin; D000077185:Resveratrol