title: Gene expression profile favoring phenotypic reversion: a clue for mechanism of tumor suppression by NF-IL6 3'UTR.
abstract: Transfection of cDNA in 3'untranslated region of human nuclear factor for interleukin-6 (NF-IL6 3'UTR) induced tumor suppression in a human hepatoma cell line. cDNA array analysis was used to reveal changes in gene expression profile leading to tumor suppression The results indicate that this suppression was not due to activation of dsRNA-dependent protein kinase, nor to inactivation of oncogenes; rather, all the changes in expression of known genes, induced by NF-IL6 3'UTR cDNA may be ascribed to the suppression of cellular malignancy. Therefore, our results imply that this 3'untranslated region may have played role of a regulator of gene expression profile.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. dgliu@sibs.ac.cn
authors.forename: Ding Gan
authors.initials: DG
authors.lastname: Liu
authors.forename: Qiu Hong
authors.initials: QH
authors.lastname: Jiang
authors.forename: Yun Yi
authors.initials: YY
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Bei Bei
authors.initials: BB
authors.lastname: Fu
authors.forename: Fu Kun
authors.initials: FK
authors.lastname: Zhao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions / Q000378:metabolism*; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics*; D015197:Carcinogenicity Tests; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002999:Clone Cells; D018076:DNA, Complementary / Q000032:analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D020413:3' Untranslated Regions; D022782:CCAAT-Enhancer-Binding Protein-beta; D018076:DNA, Complementary