title: The inhibitory effect of trilinolein on norepinephrine-induced beta-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes.
abstract: The myocardial protective effects of trilinolein, isolated from the traditional Chinese herb Sanchi (Panax notoginseng), are thought to be related to its antioxidant activity. However, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. In the present study, we investigated the effect of trilinolein on norepinephrine (NE)-induced protein synthesis in cardiomyocytes. Cultured neonatal rat cardiomyocytes were stimulated with NE, then protein content, [(3)H]-leucine incorporation, and beta-myosin heavy chain (beta-MyHC) promoter activity were examined. The effect of trilinolein on NE-induced intracellular reactive oxygen species (ROS) generation was measured with a redox- sensitive fluorescent dye (2',7'-dichlorofluorescin diacetate) and extracellular signal-regulated kinase (ERK) phosphorylation by Western blotting. Trilinolein inhibited NE-increased protein synthesis, beta-MyHC promoter activity, and intracellular ROS. Both trilinolein and the antioxidant, N-acetyl-cysteine, decreased NE- and H(2)O(2)-induced protein synthesis, beta-MyHC promoter activity, and ERK phosphorylation. These data indicate that trilinolein inhibits NE-induced protein synthesis via attenuation of ROS generation in cardiomyocytes.
authors.affiliation: Graduate Institute of Medical Sciences, Taipei Medical University, Wan Fang Hospital, No. 111 Hsing-Lung Road, Sec. 3, Wen Shan District, Taipei 117, Taiwan, ROC.
authors.forename: Ju-Chi
authors.initials: JC
authors.lastname: Liu
authors.forename: Paul
authors.initials: P
authors.lastname: Chan
authors.forename: Jin-Jer
authors.initials: JJ
authors.lastname: Chen
authors.forename: Horng-Mo
authors.initials: HM
authors.lastname: Lee
authors.forename: Wen-Sen
authors.initials: WS
authors.lastname: Lee
authors.forename: Neng-Lang
authors.initials: NL
authors.lastname: Shih
authors.forename: Yen-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Hong-Jye
authors.initials: HJ
authors.lastname: Hong
authors.forename: Tzu-Hurng
authors.initials: TH
authors.lastname: Cheng
mesh_terms: D000111:Acetylcysteine / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry / Q000494:pharmacology*; D024723:Ventricular Myosins / Q000235:genetics* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D016166:Free Radical Scavengers; D016877:Oxidants; D017382:Reactive Oxygen Species; D014280:Triglycerides; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases; D024723:Ventricular Myosins; D018995:Myosin Heavy Chains; C009564:trilinolein; D000111:Acetylcysteine; D009638:Norepinephrine