(-)-3,5-Dicaffeoyl-muco-quinic acid isolated from Aster scaber contributes to the differentiation of PC12 cells: through tyrosine kinase cascade signaling.
Aster scaber T. (Asteraceae) has been used in traditional Korean and Chinese medicine to treat bruises, snakebites, headaches, and dizziness. (-)-3,5-Dicaffeoyl-muco-quinic acid (DQ) isolated from A. scaber induced neurite outgrowth in PC12 cells. It has been reported that the activation of the extracellular signal regulated kinase 1/2 (Erk 1/2) and phosphoinositide 3 (PI3) kinase plays a crucial role in the NGF-induced differentiation of PC12 cells. This study showed that the effect of DQ on neurite outgrowth is mediated via the Erk 1/2 and PI3 kinase-dependent pathways like NGF. Furthermore, DQ stimulated the phosphorylation of Trk A. Overall, DQ elicited the differentiation of PC12 cells through Trk A phosphorylation followed by Erk 1/2 and PI3 kinase activation.
Hur
Jin Young
JY
Department of Herbal Pharmacology, Graduate School of East-West Medical Science, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Lee
Pyeongjae
P
Kim
Hocheol
H
Kang
Insug
I
Lee
Kang Ro
KR
Kim
Sun Yeou
SY
D000818:Animals; D031173:Aster Plant / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D020917:Receptor, trkA / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D002726:Chlorogenic Acid; D020917:Receptor, trkA; C100434:3,5-dicaffeoylquinic acid