Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus.
Resistin is an adipocyte-derived peptide that might play a role in obesity and insulin resistance. However, its role in humans is largely unclear. Although many studies have measured the expression of human resistin in tissues, the circulating concentrations of resistin and its relation to metabolic parameters in humans are unknown. We developed an ELISA for human resistin and measured plasma concentrations in aged individuals with or without type 2 diabetes mellitus. To validate the results of plasma resistin concentrations in our subjects, plasma adiponectin concentrations were also determined, which were higher in nondiabetic subjects than in type 2 diabetic patients and correlated with the homeostasis model assessment for insulin resistance (HOMA-IR). Log-transformed plasma resistin concentrations (log-resistin) were higher in diabetic patients compared with normal individuals (0.50 +/- 0.39 vs. 0.28 +/- 0.51 ng/ml; P < 0.001), and this difference was significant after controlling for gender and body mass index. Log-resistin did not show a significant correlation with HOMA-IR, waist circumference, body mass index, blood pressure, or total cholesterol. The plasma glucose concentration was an independent factor associated with log-resistin. In conclusion, plasma resistin concentrations are elevated in patients with type 2 diabetes, but are not associated with insulin resistance or obesity.
Youn
Byung-Soo
BS
KOMED Institute for Life Science, Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea.
Yu
Kang-Yeol
KY
Park
Hong Je
HJ
Lee
Nam Seok
NS
Min
Sung Shik
SS
Youn
Moon Yeon
MY
Cho
Young Min
YM
Park
Young Joo
YJ
Kim
Seong Yeon
SY
Lee
Hong Kyu
HK
Park
Kyong Soo
KS
D000368:Aged; D000911:Antibodies, Monoclonal; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D001824:Body Constitution; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006706:Homeostasis; D006729:Hormones, Ectopic / Q000097:blood*; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D052243:Resistin
D000911:Antibodies, Monoclonal; D001786:Blood Glucose; D006729:Hormones, Ectopic; C499624:RETN protein, human; D052243:Resistin; D002784:Cholesterol