Platelet microparticles induce angiogenesis in vitro.
Platelet microparticles (PMP) are endogenous substances generated during the coagulation process in a hypercoagulable state. This study demonstrated that PMP promote the proliferation and survival, migration, and tube formation in human umbilical vein endothelial cells (HUVEC). Heat-treated PMP did not significantly decrease the angiogenic activity in HUVEC compared with that of the untreated PMP. Meanwhile when PMP were treated with activated charcoal, a procedure known to remove the lipid growth factors, the angiogenic activity was significantly reduced. These results suggest that the lipid component(s) of the PMP may be major active factor(s) and that protein component(s) may be minor contributor(s). PMP were also shown to augment endothelial progenitor cell differentiation in peripheral blood mononuclear cells. In addition, PMP-stimulated proliferation, chemotaxis and tube formation of the HUVEC was mediated via the Pertussis toxin-sensitive G protein, extracellular signal-regulated kinase and the phosphoinositide 3-kinase pathway. Herein, a new action of PMP was demonstrated to be a potent angiogenic stimulator. It is expected that in pathological states such as a growing tumour, PMP shed from the circulating platelets may reach adequate concentrations and that the elevated levels of PMP could contribute to florid formation of new blood vessels.
Kim
Hyun Kyung
HK
Hematologic Malignancies Branch, Division of Special Cancers, Research Institute & Hospital, National Cancer Centre, 809 Madu, Ilsan, Goyang, Gyeonggi 411-769, Republic of Korea. lukekhk@ncc.re.kr
Song
Kyung Soon
KS
Chung
Jun-Ho
JH
Lee
Kyoung Rhan
KR
Lee
Se-Na
SN
D017209:Apoptosis; D001777:Blood Coagulation; D001792:Blood Platelets; D002196:Capillaries / Q000166:cytology; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D002633:Chemotaxis; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic; D013234:Stem Cells / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D042461:Vascular Endothelial Growth Factor A