title: PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers.
abstract: In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5'-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.
authors.affiliation: Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
authors.forename: Roman
authors.initials: R
authors.lastname: Kotłowski
authors.forename: Isdore Chola
authors.initials: IC
authors.lastname: Shamputa
authors.forename: Nabil Abdullah
authors.initials: NA
authors.lastname: El Aila
authors.forename: Anna
authors.initials: A
authors.lastname: Sajduda
authors.forename: Leen
authors.initials: L
authors.lastname: Rigouts
authors.forename: Armand
authors.initials: A
authors.lastname: van Deun
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D016172:DNA Fingerprinting; D017931:DNA Primers; D004251:DNA Transposable Elements; D005838:Genotype; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements