Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.
An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and uninfected horses. The agreement with enzyme-linked immunosorbent assay results was 96.7% in the detection of field sera. The results suggest that BeICT is rapid, simple, reliable, and suitable for use to detect B. equi infection in the field.
Huang
Xiaohong
X
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Xuan
Xuenan
X
Xu
Longshan
L
Zhang
Shoufa
S
Yokoyama
Naoaki
N
Suzuki
Naoyoshi
N
Igarashi
Ikuo
I
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001404:Babesiosis / Q000175:diagnosis / Q000662:veterinary*; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D015800:Protozoan Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000913:Antibodies, Protozoan; C111468:EMA-2 protein, Babesia equi; D015800:Protozoan Proteins; D011994:Recombinant Proteins