Modified location of the major histocompatibility protein Kb by co-delivery with VP22 protein.
We report the construction of an expression vector pVP22::Kb that encodes a chimeric protein composed of VP22 and murine major histocompatibility complex class I molecule, Kb. Flow cytometry assays show that VP22 modifies normal cell surface localization of Kb protein.
Barbé-Tuana
Florencia María
FM
Programa de Pós-Graduação em Ciências Médicas: Nefrologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Machado
Denise Cantarelli
DC
Saitovitch
David
D
D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection / Q000379:methods*; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism*
D015395:Histocompatibility Antigens Class I; D015778:Minor Histocompatibility Antigens; C490628:Mr1 protein, mouse; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; C434023:tegument protein VP22, bovine herpesvirus 1