Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.
Tamaki
Yoh
Y
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Hirata
Haruyuki
H
Takabatake
Noriyuki
N
Bork
Sabine
S
Yokoyama
Naoaki
N
Xuan
Xuenan
X
Fujisaki
Kozo
K
Igarashi
Ikuo
I
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001403:Babesia / Q000235:genetics* / Q000276:immunology; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017125:Genes, Protozoan; D006734:Horse Diseases / Q000175:diagnosis* / Q000276:immunology; D006736:Horses; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins