[Relationship between floral differentiation and endogenous hormones and polyamines contents in cucumber cotyledonary nodes cultured in vitro].
Changes of endogenous hormones and polyamines(PAs) contents during floral differentiation (0-6 day) in cucumber cotyledonary nodes cultured in vitro were determined by using HPLC. The results showed that all four endogenous hormones decreased obviously within 0-2 day and increased slightly within 4-5 day after culture. This indicated that low levels of indole-3-acetic acid (IAA), gibberelline(GA3) and abscisin acid(ABA) during 0-2 day were favorable to floral primordia differentiation, while high level of zeatin(ZT) during 3-5 day was favorable to floral organ primordia differentiation. Contents of spermine(Spm), spermidine(Spd) and cadavarine(Cad) were decreased within 0-1 day, but increased within 1-4 day, and then decreased again within 4-5 day after culture. On the contrary, content of putrescine(Put) was increased significantly on the first day and then declined during 1-6 day. Changes of PAs contents indicated that high levels of PAs and Put were favorable to floral primordia differentiation, the increasing of Spm after the second day was favorable to floral organ primordia differentiation, and change of Cad content may be one of the characteristic symbols between floral and vegetative bud differentiation.
Wang
Li Lin
LL
College of Life Sciences, Zhejiang University, School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
Pang
Ji Liang
JL
Hu
Jiang Qin
JQ
Zhu
Mu Yuan
MY
Liang
Hai Man
HM
D015317:Biogenic Polyamines / Q000378:metabolism*; D018553:Cucumis sativus / Q000254:growth & development* / Q000378:metabolism*; D046508:Culture Techniques; D035264:Flowers / Q000254:growth & development / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D015026:Zeatin / Q000378:metabolism
D015317:Biogenic Polyamines; D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D013096:Spermine; C030737:indoleacetic acid; D015026:Zeatin; C007842:gibberellic acid; D013095:Spermidine; D011700:Putrescine