[Bone formation in vitro and in vivo by human bone marrow-derived mesenchymal stem cells].
OBJECTIVE
To culture and study the osteogenic characteristics of human bone marrow-derived mesenchymal stem cells (hBMMSCs).


METHODS
hBMMSCs were separated and cultured from human iliac crest marrow. Growth kinetics of hBMMSCs was studied by growth curve. Under the osteoinductive culture, osteogenic differentiation of hBMMSCs was tested by alkaline phosphatase (ALP). Osteogenic functions of hBMMSCs in vitro and in vivo were also respectively detected by von Kossa stain and by transplanting hydroxyapatite/tricalcium phosphate ceramics (HA/TCP) with hBMMSCs.


RESULTS
hBMMSCs were cultured successfully. The growth curve of the second passage of BMMSCs indicated that the time of population doublings was about 3.5 days. The results of ALP stain were evident by the significant increase in ALP activity after hBMMSCs cultured in osteoinductive medium. Some mineralized nodules were detected by von Kossa stain at nineteenth day of osteoinductive culture. In vivo assay, histological evalution showed bone formation in 3 months after grafts of HA/TCP with hBMMSCs.


CONCLUSIONS
Osteoinductive solution can induce hBMMSCs to differentiate osteogenetic cell lines. Mineralized nodules and bone formation were found in vitro and in vivo assay. The results demonstrate that hBMMSCs have the potential for osteogenesis.
Wang
Yi-xiang
YX
Research Laboratory of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China.
Li
Sheng-lin
SL
Wu
Deng-cheng
DC
Zhang
Kui-hua
KH
Yu
Shi-feng
SF
Cui
Sheng-xian
SX
D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010012:Osteogenesis
D000469:Alkaline Phosphatase