title: Analysis of a nonribosomal peptide synthetase gene from Alternaria brassicae and flanking genomic sequences.
abstract: Very little information is currently available concerning the pathogenic determinants produced by Alternaria brassicae, the causal agent of the blackspot disease of crucifers. We screened a genomic library of this fungus and identified a nonribosomal peptide synthetase (NRPS) gene named AbrePsy1. The complete coding sequence is 22 kbp long and encodes a large protein (792 kDa) showing typical NRPS modular organization. Structural analysis of AbrePsy1 revealed four complete elongation modules, two of which have epimerization domains. In the vicinity of AbrePsy1, a second gene (named AbreAtr1), which encodes an ATP-binding cassette transporter was identified. Increased expression of AbrePsy1 and AbreAtr1 was observed during host-plant infection. However, while physically linked, these two genes are probably not functionally clustered, as their expression patterns differed.
authors.affiliation: Faculté des Sciences, UMR PAVE 77, 2 Bd Lavoisier, 49045, Angers, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Guillemette
authors.forename: Adnane
authors.initials: A
authors.lastname: Sellam
authors.forename: Philippe
authors.initials: P
authors.lastname: Simoneau
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000528:Alternaria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
chemical_list: D018528:ATP-Binding Cassette Transporters; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase