title: Simple and reliable method to precipitate proteins from bacterial culture supernatant.
abstract: A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.
authors.affiliation: CREATOGEN AG, D-86156 Augsburg, Germany.
authors.forename: Randolph B
authors.initials: RB
authors.lastname: Caldwell
authors.forename: Claus T
authors.initials: CT
authors.lastname: Lattemann
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001431:Bacteriological Techniques; D011232:Chemical Precipitation; D017077:Culture Media, Conditioned / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000737:chemistry; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D011748:Pyrogallol; C044659:molybdate; C058731:pyrogallol sulfonphthalein; D008982:Molybdenum; D000432:Methanol