Involvement of tumor necrosis factor-alpha and interleukin-8 in antigen-induced arthritis of the rabbit temporomandibular joint.
BACKGROUND
In temporomandibular joint (TMJ) arthritis, knowledge is limited about the source of the inflammatory mediators. The aim of this study was to investigate the immunohistochemical role of tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) in the development of the antigen-induced arthritis of the rabbit TMJ.


METHODS
Unilateral TMJ arthritis was induced in 28 adult rabbits. From 6 h to 6 weeks after induction of arthritis, the topology of TNF-alpha and IL-8 was observed.


RESULTS
Positive reaction for TNF-alpha of synovial cells was observed within 3 days after induction and at 3 weeks after induction. TNF-alpha positive vascular endothelial cells and chondrocytes were identified throughout the observation period. IL-8 was detected only during the acute stage.


CONCLUSIONS
The cytokines TNF-alpha and IL-8 were observed in specific cells depending on the stage. TNF-alpha was particularly related with angiogenesis and cartilage destruction and IL-8 was involved in the acute stage of inflammation.
Sukedai
Miho
M
First Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 2-6-1 Manazuru, Kitakyushu 803-8580, Japan. miho-s@kyu-dent.ac.jp
Tominaga
Kazuhiro
K
Habu
Manabu
M
Matsukawa
Akihiro
A
Nishihara
Tatsuji
T
Fukuda
Jinichi
J
D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology; D019902:Chondrocytes / Q000378:metabolism; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D009389:Neovascularization, Pathologic; D011817:Rabbits; D013705:Temporomandibular Joint Disorders / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha