title: Increased CD11b neutrophil expression in Budd-Chiari syndrome or portal vein thrombosis secondary to polycythaemia vera.
abstract: Budd-Chiari syndrome and portal vein thrombosis (BCS/PVT) are frequently associated with polycythaemia vera (PV). In an attempt to elucidate the mechanisms of BCS/PVT secondary to PV (T-PV), CD11b neutrophil expression, neutrophil oxidative burst and platelet-neutrophil complexes (PNC) were assessed in 17 such patients. Three groups served as controls: BCS/PVT not secondary to PV (T-nPV; n = 20), PV without thrombosis (PV-nT; n = 16), and healthy controls (HC; n = 20). Baseline CD11b expression (in mean fluorescence intensity units) was 101 [95% confidence interval (CI): 79-128] in T-PV patients, versus 25 (95% CI: 18-35) in T-nPV, 59 (95% CI: 43-80) in PV-nT, and 34 (95% CI: 25-48) in HC (P < 0.001). After N-formyl-L-methionyl-L-leucyl-L-phenylalanine activation, T-PV patients also showed higher CD11b values: 190 (95% CI: 151-238), versus 55 (95% CI: 41-72) in T-nPV, 111 (95% CI: 81-153) in PV-nT, and 77 (95% CI: 63-95) in HC (P < 0.001). In BCS/PVT, CD11b neutrophil expression had 90% specificity and 100% sensitivity for the association with PV. Finally, PV patients had higher oxidative burst and PNC than T-nPV patients or HC (P < 0.05). These results support a role for neutrophils in BCS/PVT secondary to PV and indicate that neutrophil CD11b expression could be of use for PV screening in BCS/PVT patients.
authors.affiliation: Haematology Department, Institute of Haematology and Oncology, Hospital Clínic, IDIBAPS, University of Barcelona, Villaroel 170, 08036 Barcelona, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Alvarez-Larrán
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: García-Pagán
authors.forename: Juan G
authors.initials: JG
authors.lastname: Abraldes
authors.forename: Eduardo
authors.initials: E
authors.lastname: Arellano
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Reverter
authors.forename: Jaume
authors.initials: J
authors.lastname: Bosch
authors.forename: Francisco
authors.initials: F
authors.lastname: Cervantes
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis; D006502:Budd-Chiari Syndrome / Q000209:etiology / Q000276:immunology*; D039481:CD11b Antigen / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D011087:Polycythemia Vera / Q000150:complications / Q000276:immunology*; D011169:Portal Vein; D012372:ROC Curve; D020246:Venous Thrombosis / Q000209:etiology / Q000276:immunology
chemical_list: D015415:Biomarkers; D039481:CD11b Antigen