title: [Functional reconstruction with tissue engineered myoblast in facial muscle of rat].
abstract: OBJECTIVE
  The purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.
  
  
  METHODS
  Using purified, subcultured myoblast of neonatal rats and type I collagen gels as extracellular matrix (ECM) and scaffold, tissue engineered muscle was transplanted in the face of syngeneic nutured rats.
  
  
  RESULTS
  Tissue engineered myoblast generated and differentiated in vitro were observed with microscope. Myoblast fused each other and formed myofibers in the face of rats, nerve fibers and vessels regeneration could also be found in some samples. Postoperative electromiographs showed the myofibers were active when stimulating the nerve trunk that innervates the engineered facial muscle.
  
  
  CONCLUSIONS
  Tissue engineered method is hopeful to be used as a new technique to reconstruct defects of facial muscle.
authors.affiliation: Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Huang
authors.forename: Longjiang
authors.initials: L
authors.lastname: Li
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D005152:Facial Muscles / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D005260:Female; D005782:Gels; D008297:Male; D032446:Myoblasts / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D005782:Gels