title: Enhanced oxidative stress and impaired thioredoxin expression in spontaneously hypertensive rats.
abstract: As oxidative stress plays a crucial role in the development and pathogenesis of hypertension, we analyzed the redox (reduction/oxidation) status in tissues from Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and stroke-prone SHR (SHRSP). Expressions of 8-hydroxy-2'-deoxyguanosine, a marker for oxidative stress-induced DNA damage, and protein carbonylation, a marker for oxidation status of proteins, were enhanced in aorta, heart, and kidney from SHR and SHRSP compared with WKY. The expression of redox regulating protein, thioredoxin (TRX), estimated by immunohistochemistry and western blot, and expression of TRX gene estimated by real-time RT-PCR were markedly suppressed in those tissues from SHR and SHRSP compared with WKY. Induction of TRX was impaired after angiotension II treatment in peripheral blood mononuclear cells isolated from SHR and SHRSP compared with those isolated from WKY. Although previous reports have shown that TRX is induced by a variety of oxidative stress in tissues, the present study shows the impaired induction of TRX in tissues from genetically hypertensive rats despite the relative increment of oxidative stress. Redox imbalance in essential organs may play a crucial role in the development and pathogenesis of hypertension.
authors.forename: Masaki
authors.initials: M
authors.lastname: Tanito
authors.forename: Hajime
authors.initials: H
authors.lastname: Nakamura
authors.forename: Yong-Won
authors.initials: YW
authors.lastname: Kwon
authors.forename: Akie
authors.initials: A
authors.lastname: Teratani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masutani
authors.forename: Keisuke
authors.initials: K
authors.lastname: Shioji
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kishimoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohira
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Horie
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013879:Thioredoxins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D000804:Angiotensin II; D013879:Thioredoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine