[The methodological specificity of PCR-test of gene p53 in plasma and blood cell DNA of oncological patients].
The evaluation of mutations in the p53 gene is of a big value for the laboratory diagnostics in oncology. The p53 gene was analyzed, within the present case study, in blood plasma, in which tumor-origin DNAs were detected, as well as in blood cells and in tumor tissue. Optimal conditions were defined for polymerase chain reaction (PCR) with primers. The adequacy of the conditions selected for PCR and for SSCP-electrophoresis, when used in the analysis of mutations in exons 5-8, gene p53, was shown by the method of direct sequencing. The first results on revealing the frequency of gene-p53 mutations were obtained for some of the oncology diseases in the studied population.
Bartnovskiĭ
A E
AE
Belokhvostov
A S
AS
Abramov
A A
AA
Likhin
F A
FA
D001773:Blood Cells / Q000378:metabolism; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D005091:Exons; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000097:blood / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded