[Isolation and purification of bovine testicular hyaluronidase].
We developed a single-step method for chromatographic purification of the hyaluronidase extract on Sepharose blue, which produced high yield of the enzyme (95%) with high purity (40). Purification of hyaluronidase allowed us to obtain biological preparations standardized by the protein composition and enzyme activity and useful for basic and applied researches.
Barsukov
A K
AK
Udmurt State University, Izhevsk, 426034 Russia.
Kozhevnikova
O V
OV
Khokhriakova
A V
AV
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification*; D008297:Male; D011786:Quality Control; D012685:Sepharose; D013737:Testis / Q000201:enzymology*
D012685:Sepharose; D006821:Hyaluronoglucosaminidase