Analysis of radiation-induced hydrocarbons and 2-alkylcyclobutanones from dried shrimps (Penaeus aztecus).
We investigated the usefulness of hydrocarbons and 2-alkylcyclobutanones as markers for irradiated, dried shrimps. A method was developed to detect the irradiation of dried shrimps (Penaeus aztecus) by identifying radiation-induced hydrocarbons and 2-alkylcyclobutanones extracted from dried shrimps, which were separated by Florisil column chromatography and identified by a method using gas chromatography/mass spectrometry. Radiation-induced hydrocarbons and 2-alkylcyclobutanones are formed from the fatty acids of the irradiated fats. The quantity of the hydrocarbons and 2-alkylcyclobutanones formed from some fatty acids is related to the composition of fatty acids in a sample. The concentrations of hydrocarbons and 2-alkylcyclobutanones increased with radiation dose. The major hydrocarbons in the irradiated, dried shrimps were 1-tetradecene and pentadecane from palmitic acid; small amounts of heptadecane and 1-hexadecene from stearic acid; and 8-heptadecene and 1,7-hexadecadiene from oleic acid. 2-(5'-Tetradecenyl)cyclobutanone, one of the 2-alkylcyclobutanones, was present at the highest concentration. The radiation-induced hydrocarbons and 2-alkylcyclobutanones were detected at radiation doses of 0.5 kGy and greater. These compounds were not detected in nonirradiated, dried shrimps.
Kim
Kyong-Su
KS
Department of Food and Nutrition, Chosun University, Gwangju, Korea 501-759. kskim@mail.chosun.ac.kr
Seo
Hye-Young
HY
Lee
Jeong-Min
JM
Park
Eun-Ryong
ER
Kim
Jun-Hyoung
JH
Hong
Cheul-Hee
CH
Byun
Myung-Woo
MW
D000818:Animals; D002074:Butanones / Q000032:analysis*; D004307:Dose-Response Relationship, Radiation; D005227:Fatty Acids / Q000528:radiation effects; D005514:Food Irradiation; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000032:analysis*; D033561:Penaeidae / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage
D002074:Butanones; D005227:Fatty Acids; D006838:Hydrocarbons