title: [Colony-stimulating factor-1 receptor in rat dental follicle cells].
abstract: OBJECTIVE
  To study localization and expression of CSF-1 receptor protein, in order to discover the CSF-1 and IL-1alpha effects on CSF-1 receptor mRNA levels and to determine if the autocrine effect is inhibited through the CSF-1 receptor.
  
  
  METHODS
  Immunolocalization of CSF-1 receptor in the cultured dental follicle cells and in mandibles of the post-natal rats from day 1 to 11 were performed. The effects of different concentrations of CSF-1, IL-1alpha on CSF-1 receptor gene expression were detected by means of RT-PCR.
  
  
  RESULTS
  Cultured dental follicle cells were immunostained for the CSF-1 receptor. In vivo, immunostaining showed that the CSF-1 receptor was present in the dental follicle of the first mandibular molar at early post-natally and was either absent or greatly reduced by day 11 post-natally. High concentrations of cvCSF-1 reduced the gene expression of the CSF-1 receptor. IL-1alpha had no effects on CSF-1 receptor mRNA levels.
  
  
  CONCLUSIONS
  The expression of CSF-1 receptor reaches a peak early post-natally in the dental follicle of the first mandibular molar of the rat and then subsequently declines. High concentrations of CSF-1 inhibits the expression of CSF-1 receptor, IL-1alpha has no effect on the expression of CSF-1 receptor mRNA.
authors.affiliation: Department of Operative and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
authors.forename: Hai-jing
authors.initials: HJ
authors.lastname: Gu
authors.forename: Jun-qi
authors.initials: JQ
authors.lastname: Ling
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003795:Dental Sac / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis* / Q000235:genetics
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor