title: Assessment of expressions of heat shock protein (HSP 72) and apoptosis after ArF excimer laser ablation of the cornea.
abstract: We immunohistochemically studied expressions of inducible heat shock protein 70 (HSP 72) and apoptosis of corneas ablated with an ArF excimer laser. The temperature of corneal surfaces and laser-induced optical emission spectra were measured in real time as direct physical parameters related to the ablation mechanism. To the best of the authors' knowledge, there have been no experimental studies regarding the influence of physical parameters directly related to the ablation mechanism on corneal reactions at the cell level after laser ablation. The expression of HSP 72 was mainly localized in the regenerative epithelium, which was confirmed to be caused by laser ablation. The HSP 72 positive cell ratios had a correlation with thermal dose, which was derived from the measured time courses of temperature. Expressions of both HSP 72 and apoptosis depended on the thermal dose and elapsed time after ablation. HSP 72 and apoptosis could be seen up to a few hundred micrometers into the stroma, only at a fluence with an optical breakdown emission. This could have been caused by shock waves induced by the optical breakdown.
authors.affiliation: Department of Medical Engineering, National Defense Medical College, Saitama, Japan. kobako@cc.ndmc.ac.jp
authors.forename: Miya
authors.initials: M
authors.lastname: Ishihara
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sato
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Arai
authors.forename: Minoru
authors.initials: M
authors.lastname: Obara
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001128:Argon; D001831:Body Temperature / Q000502:physiology; D003315:Cornea / Q000378:metabolism* / Q000528:radiation effects* / Q000601:surgery; D004307:Dose-Response Relationship, Radiation; D005459:Fluorides; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D017075:Laser Coagulation / Q000379:methods*; D007834:Lasers; D011817:Rabbits; D011829:Radiation Dosage; D013817:Thermography / Q000379:methods; D014018:Tissue Distribution
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C047730:argon fluoride; D001128:Argon; D005459:Fluorides