title: Effects of viscoelastic material on the corneal endothelial cells in trabeculectomy with adjunctive mitomycin-C.
abstract: We evaluated the change of corneal endothelium after trabeculectomy with mitomycin-C soaking (0.2 mg/ml, 2.4 +/- 1.2 minutes) and the effect of viscoelastic material in reducing this change. In randomly selected cases, 0.05 ml of sodium hyaluronate(Healon) was injected into anterior chamber (Healon group, n = 20), and same amount of balanced salt solution was injected in the other eyes (Control, n = 18) at the beginning of surgery. There were no differences in clinical variables and specular microscopy result between 2 groups before surgery. After surgery, the change of endothelial cells were significantly reduced in Healon group (cell density; -2.5 +/- 1.6%, variability of cell area; 5.8 +/- 2.5%, and percentage of hexagonal cell; -2.2 +/- 0.7%) compared to control group (-7.7 +/- 6.0%, 8.9 +/- 4.4%, and -4.8 +/- 3.5% respectively, p < 0.01). In trabeculectomy with mitomycin-C, the damage to the corneal endothelium was reduced significantly by injecting the viscoelastic material without significant complication.
authors.affiliation: Department of Ophthalmology, College of Medicine, Chungnam National University, Daejon, Korea.
authors.forename: Dong-Bin
authors.initials: DB
authors.lastname: Shin
authors.forename: Seung-Bok
authors.initials: SB
authors.lastname: Lee
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Kim
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry; D000287:Administration, Topical; D000328:Adult; D000368:Aged; D000963:Antimetabolites / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D004548:Elasticity; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D005260:Female; D005901:Glaucoma / Q000601:surgery*; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D007430:Intraoperative Care; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000008:administration & dosage*; D012189:Retrospective Studies; D014130:Trabeculectomy; D014783:Viscosity
chemical_list: D000276:Adjuvants, Immunologic; D000963:Antimetabolites; D016685:Mitomycin; D006820:Hyaluronic Acid