Characteristics of chitosanases from Aspergillus fumigatus KB-1.
Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were 60 degrees C for chitosanase land 70 degrees C for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.
Eom
Tae Kyoung
TK
College of Pharmacy, Ewha Womans University, Seoul, 120-750, Korea.
Lee
Kang Man
KM
D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000302:isolation & purification; D002686:Chitin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D048271:Chitosan; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism
D010446:Peptide Fragments; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase