Fluorescence detection of guanine-adenine transition by a hydrogen bond forming small compound.
In combination with abasic site-containing oligodeoxynucleotides, 2-amino-4-oxopteridine (pterin) can selectively recognize guanine base over other nucleobases accompanied by fluorescence quenching, which allows clear detection of a guanine-adenine transition with the naked eye.
Yoshimoto
Keitaro
K
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Xu
Chun-Yan
CY
Nishizawa
Seiichi
S
Haga
Takanobu
T
Satake
Hiroyuki
H
Teramae
Norio
N
D000225:Adenine / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D006147:Guanine / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011621:Pteridines / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013696:Temperature
D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D011621:Pteridines; D006147:Guanine; D000225:Adenine