Ultracytochemical demonstration of glycoproteins in the canine knee synovium.
By various ultracytochemical methods, glycoconjugates of the synoviocytes, the intercellular matrix and the wall of the small capillaries were studied in the synovial intimal tissues of the canine knee joint. Glycoconjugates with vicinal diol groups could be visualized in certain elements of the Golgi complex, lysosomes, vacuoles, the majority of intracellular cytomembranes, the surface coat of the plasma membrane and glycogen particles in type A cells. In type B cells, less-developed Golgi complexes, and fewer lysosomes and vacuoles were present in the cytoplasm than in that of type A cells. In contrast, a large number of cytoplasmic glycogen particles and abundant vicinal diol-containing groups in the surface coat of the plasma membrane became especially obvious in the B cells. Abundant neutral and acidic glycoproteins were observed in fibrous components in the intercellular matrix. In the small capillaries, strongly positive staining intensities for neutral and acidic glycoconjugates were observed in the basement membrane and perivascular connective tissue, as well as in the surface coat of the luminal plasma membrane of the endothelial cells, although to a somewhat weaker degree. Sialic acid, particularly, was notable in the surface coat of the latter cells. In addition, glycoproteins in the type A cells were shown by lectin ultracytochemistry to contain a variety of saccharide residues such as alpha-D-mannose, alpha-D-glucose, alpha-L-fucose, N-acetyl-beta-D-glucosamine, and N-acetyl-neuraminic acid, which were also found in the plasma membrane of the B cells. The properties of the glycoconjugates found are discussed in relation to the basic functions assigned to the synovial membrane of the canine knee joint.
Nagaoka
Daisuke
D
Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Tsukise
Azuma
A
Meyer
Wilfried
W
Hirabayashi
Yoshifumi
Y
D000818:Animals; D002196:Capillaries / Q000648:ultrastructure; D004285:Dogs / Q000033:anatomy & histology*; D005552:Forelimb; D006001:Glycoconjugates / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D007425:Intracellular Membranes / Q000648:ultrastructure; D007596:Joints / Q000648:ultrastructure; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D013583:Synovial Membrane / Q000098:blood supply / Q000166:cytology* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure
D006001:Glycoconjugates; D006023:Glycoproteins