Altered autonomic control in rat intestine due to both infection with Anisakis simplex and incubation with the parasite's crude extract.
Anisakis simplex IgE may bring on allergic responses such as angioedema, vomiting, and urticaria from eating seafood, but it is not the only etiology. Induced cholinergic hyperreactivity or adrenergic blockade in the target tissue can cause these diseases nonimmunologically also. Here we studied the effects on normal intestinal motility of brief A. simplex infections and in vitro exposures to the parasite's extract (CE). Each approach was evaluated according to its ability to induce cholinergic hyperreactivity or adrenergic blockade in rat duodenum (RD), jejunum (RJ), and ileum (RI) in vitro. Additionally, bolus propulsion in RD, RJ, and RI was evaluated with time in vivo utilizing animals infected 4 h previously with A. simplex larvae (L3) vs sham animals. Tissues, after inoculation of 1, 5, 10, and 20 L3, exhibited time- and dose-dependent motility changes after carbachol (Ch) and noradrenaline (NA), justifying our using herein rats from the fourth hour of infection with 20 L3. We observed a persistent, yet differential effect of the infection on RD, RJ, and RI responses to Ch or NA. It caused cholinergic (muscarinic) hyperreactivity in RD only, and adrenergic blockade in all other parts, and consequently increased the transit index in RD, not in RJ or RI. In contrast, exposing RD, RJ, and RI to CE persistently increased both parameters, amplitude of twitches and muscular tone, in all, albeit that, here also, responses to Ch and NA were CE dose dependent. Interestingly, sensitivity to CE was in the order RI > RJ > RD, the reverse situation of that observed during active infection. Thus, previously viable A. simplex L3, after digestion, can exert bystander disturbance in autonomic control in the whole intestine. Our findings demonstrate that A. simplex L3, alive or dead, can induce cholinergic hyperactivity and adrenergic blockade in the whole small intestine and, as a consequence, gastrointestinal symptoms. Significantly, they may do so long before parasite-specific IgE is detectably induced or despite the occurrence of such IgE.
Sánchez-Monsálvez
I
I
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
De Armas-Serrá
C
C
Bernadina
W
W
Rodríguez-Caabeiro
F
F
D000316:Adrenergic alpha-Agonists / Q000008:administration & dosage; D000707:Anaphylaxis / Q000209:etiology / Q000503:physiopathology; D000799:Angioedema / Q000209:etiology / Q000503:physiopathology; D000818:Animals; D017129:Anisakiasis / Q000503:physiopathology*; D017163:Anisakis / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity*; D000909:Antibodies, Helminth / Q000032:analysis; D002217:Carbachol / Q000008:administration & dosage; D018679:Cholinergic Agonists / Q000008:administration & dosage; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000187:drug effects / Q000503:physiopathology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007082:Ileum / Q000187:drug effects / Q000503:physiopathology; D007411:Intestinal Diseases, Parasitic / Q000503:physiopathology*; D007422:Intestines / Q000187:drug effects / Q000469:parasitology / Q000503:physiopathology*; D007583:Jejunum / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D017747:Seafood / Q000469:parasitology; D014020:Tissue Extracts / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D000909:Antibodies, Helminth; D018679:Cholinergic Agonists; D014020:Tissue Extracts; D002217:Carbachol; D009638:Norepinephrine