title: [Clinical significance and correlation between elevated serum TR6 and lympho-metastasis in gastric cancer].
abstract: OBJECTIVE
  To evaluate the value of serum TR(6) for the diagnosis and TNM classification in patients with gastric carcinoma.
  
  
  METHODS
  Serum TR(6) levels were measured using ELISA method in 31 gastric cancer patients, 19 patients with nonmalignant conditions and 29 healthy individuals. TR(6) expression in tumor mass was studied with immunohistochemistry. TR(6) gene copy number in tumor tissues was evaluated by real time PCR.
  
  
  RESULTS
  Ninety-seven point nine percent (47 of 48 cases) of healthy individuals and patients with nonmalignant conditions were serum TR(6)-negative. In contrast, 71% (22 of 31 cases) of gastric cancer patients were serum TR(6)-positive. Serum TR(6) positiveness was closely correlated with tumor differentiation status and TNM classification. TR(6) gene amplification did not occur in gastric carcinoma.
  
  
  CONCLUSIONS
  Serum TR(6) levels were correlated significantly with TNM stage and histopathological type of tumor. This can help to determine the pre-operative TNM classification and to choose the optimal extent of lymph node dissection for gastric cancer.
authors.affiliation: Department of Surgery, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Wu
authors.forename: Jun-xiu
authors.initials: JX
authors.lastname: Yu
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Shen
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Shun-liang
authors.initials: SL
authors.lastname: Gao
mesh_terms: D006801:Humans; D008207:Lymphatic Metastasis; D008562:Membrane Glycoproteins / Q000097:blood*; D009367:Neoplasm Staging; D011956:Receptors, Cell Surface / Q000097:blood*; D018124:Receptors, Tumor Necrosis Factor; D053323:Receptors, Tumor Necrosis Factor, Member 6b; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology
chemical_list: D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D053323:Receptors, Tumor Necrosis Factor, Member 6b; C505911:TNFRSF6B protein, human