A nucleobase that releases reporter tags upon DNA oxidation.
We have developed a novel nucleosbase, edaG, that efficiently releases various reporter units upon one-electron oxidation. The edaG-selective degradation of ODNs was achieved by various mild oxidizing agents. The oxidant-dependent molecular releasing technique is quite useful not only for DNA-based drug releasing systems but also for the detection of long-range hole transport through DNA without time-consuming analysis.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan.
Tanaka
Kazuo
K
Saito
Isao
I
D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D005453:Fluorescence; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005029:Ethylenediamines; D009705:Nucleosides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D006147:Guanine; C031234:ethylenediamine; D004247:DNA