Decreased agglutinability of human erythrocytes by attachment of methoxy polyethylene glycol and the effect on erythrocyte oxygen-carrying ability.
In the interest of creating readily transfusable blood, we have developed a method of covalently bonding methoxy polyethylene gylcol (mPEG) to the erythrocyte membrane to mask antigens which would otherwise cause immunorejection in unmatched blood transfusions. Previous studies have reported the ability of mPEG to mask erythrocyte antigens without significantly affecting viability, morphology, deformability, and other membrane functions. Our spectrophotometric analysis of modified erythrocytes has shown that as well as its other properties, mPEG-coated red blood cells can still take up oxygen, while remaining antigenically silent.
Ong
Karolyn
K
Department of Physiology and Philippine General Hospital, University of the Philippines-College of Medicine, Ermita, Manila, Philippines.
Dimacali
Kristine
K
Quintos
Ricardo
R
D001803:Blood Transfusion; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000276:immunology; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000032:analysis*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012203:Rh Isoimmunization / Q000517:prevention & control; D012204:Rh-Hr Blood-Group System / Q000737:chemistry / Q000276:immunology
D010108:Oxyhemoglobins; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D010100:Oxygen