Detection of ischemia by PCO2 before adenosine triphosphate declines in skeletal muscle.
OBJECTIVE
Ischemia is a serious problem in clinical medicine, and effective methods are needed to detect ischemia before the injury becomes irreversible. In experimental studies on several organs, PCO2 was found to increase rapidly after the onset of supply-dependent anaerobic metabolism. A shortcoming of these studies was that PCO2 was not correlated with tissue concentrations of lactate and the energy status in the cell. Thus, in this study we have measured tissue concentrations of lactate, phosphocreatine, and adenosine triphosphate. We hypothesized that during ischemic conditions, PCO2 reflects lactate generation in the cell and not exhausted energy stores per se. If this is the case, PCO2 can be used to detect ischemia before the energy stores are depleted. Consequently, therapy can be instituted at a time when the organ is salvageable.


METHODS
Prospective laboratory study.


METHODS
University research laboratory.


METHODS
Seven pigs.


METHODS
In a porcine model, gluteal skeletal muscles with no-flow ischemia were examined. PCO2 was measured both in situ and in vitro at increasing periods of time. Concomitantly, tissue lactate, adenosine triphosphate, and phosphocreatine were analyzed.


RESULTS
Tissue surface CO2 tension (PtCO2) increased rapidly after onset of ischemia. From a baseline of 63 +/- 3 torr (8.4 +/- 1.2 kPa) under aerobic conditions, it increased to 157 +/- 6 torr (21 +/- 2.2 kPa) after 30 mins of ischemia and 386 +/- 9 torr (51.5 +/- 3 kPa) at 120 mins. The rapid increase of PtCO2 correlated well with increasing values of lactate (r2 >.9) in the tissue. Adenosine triphosphate was essentially unchanged for 45 mins after onset of ischemia, after which it declined. Phosphocreatine decreased earlier than adenosine triphosphate in accordance with the notion that high-energy phosphate groups are transferred from phosphocreatine to adenosine triphosphate.


CONCLUSIONS
In this porcine model of skeletal muscle ischemia, PtCO2 correlates well with tissue lactate and increases long before the energy stores of phosphocreatine and most notably adenosine triphosphate are severely reduced. Thus, PtCO2 could be monitored to detect and treat earlier stages of ischemia.
Kvarstein
Gunnvald
G
Department of Anesthsiology, Rikshospitalet, Oslo, Norway.
Mirtaheri
Peyman
P
Tønnessen
Tor Inge
TI
D000255:Adenosine Triphosphate / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis*; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000175:diagnosis* / Q000517:prevention & control; D007773:Lactates / Q000032:analysis; D016014:Linear Models; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000503:physiopathology; D010101:Oxygen Consumption / Q000502:physiology; D011336:Probability; D012680:Sensitivity and Specificity; D034421:Sus scrofa
D007773:Lactates; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate