Mutations including IS6110 insertion in the gene encoding the MPB64 protein of Capilia TB-negative Mycobacterium tuberculosis isolates.
A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.
Hirano
Kazue
K
Department of Basic Research, Research Institute of Tuberculosis, Fukujuji Hospital, Kiyose-shi, Tokyo 204-8533, Japan. hirano@jata.or.jp
Aono
Akio
A
Takahashi
Mitsuyoshi
M
Abe
Chiyoji
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002845:Chromatography; D004251:DNA Transposable Elements; D016254:Mutagenesis, Insertional; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C080865:MPB64 protein, Mycobacterium