title: Genetic instability in EBV-transformed lymphoblastoid cell lines.
abstract: Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.
authors.affiliation: Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories, G.P.O. Box 2891, Islamabad 44000, Pakistan.
authors.forename: Aisha
authors.initials: A
authors.lastname: Mohyuddin
authors.forename: Qasim
authors.initials: Q
authors.lastname: Ayub
authors.forename: Saima
authors.initials: S
authors.lastname: Siddiqi
authors.forename: Denise R
authors.initials: DR
authors.lastname: Carvalho-Silva
authors.forename: Kehkashan
authors.initials: K
authors.lastname: Mazhar
authors.forename: Sadia
authors.initials: S
authors.lastname: Rehman
authors.forename: Sadaf
authors.initials: S
authors.lastname: Firasat
authors.forename: Ahsana
authors.initials: A
authors.lastname: Dar
authors.forename: Chris
authors.initials: C
authors.lastname: Tyler-Smith
authors.forename: S Qasim
authors.initials: SQ
authors.lastname: Mehdi
mesh_terms: D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D041322:Chromosomes, Human, Y; D004261:DNA Replication; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation