title: Frequency of cryptosporidium in childhood diarrhoea--importance of modified acid fast technique.
abstract: BACKGROUND
  One of the important causes of childhood diarrhoea is cryptosporidium, a parasitic pathogen which is usually overlooked. This study was therefore designed to show the importance of modified acid fast stain in the diagnosis of cryptosporidium in childhood diarrhoea.
  
  
  METHODS
  Stool samples from 300 children with prolonged diarrhoea were examined by modified acid fast staining a long with wet mounts in saline and iodine.
  
  
  RESULTS
  Out of 300 samples five (1.7%) were diagnosed as positive for cryptosporidium.
  
  
  CONCLUSIONS
  Results indicate that Cryptosporidiosis exists as a cause of diarrhoeal illness in our society. It was also noted that modified acid fast staining of stool samples is an important, non-invasive and useful diagnostic technique in identification of cryptosporidium.
authors.affiliation: Department of Pathology, Dow Medical College, Karachi. shaheens@cyber.net.pk
authors.forename: Shaheen
authors.initials: S
authors.lastname: Shoaib
authors.forename: Shoaib
authors.initials: S
authors.lastname: Tauheed
authors.forename: Amtul
authors.initials: A
authors.lastname: Hafiz
mesh_terms: D000293:Adolescent; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000453:epidemiology; D003458:Cryptosporidium / Q000302:isolation & purification; D003967:Diarrhea / Q000469:parasitology*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007223:Infant; D008297:Male; D013194:Staining and Labeling / Q000379:methods