Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.
BACKGROUND
Leukotrienes are inflammatory mediators generated from arachidonic acid (polyunsaturated n-6 fatty acid) by the enzyme 5-lipoxygenase. Since atherosclerosis involves arterial inflammation, we hypothesized that a polymorphism in the 5-lipoxygenase gene promoter could relate to atherosclerosis in humans and that this effect could interact with the dietary intake of competing 5-lipoxygenase substrates.


METHODS
We determined 5-lipoxygenase genotypes, carotid-artery intima-media thickness, and markers of inflammation in a randomly sampled cohort of 470 healthy, middle-aged women and men from the Los Angeles Atherosclerosis Study. Dietary arachidonic acid and marine n-3 fatty acids (including a competing 5-lipoxygenase substrate that reduces the production of inflammatory leukotrienes) were measured with the use of six 24-hour recalls of food intake.


RESULTS
Variant 5-lipoxygenase genotypes (lacking the common allele) were found in 6.0 percent of the cohort. Mean (+/-SE) intima-media thickness adjusted for age, sex, height, and racial or ethnic group was increased by 80+/-19 microm (95 percent confidence interval, 43 to 116; P<0.001) among carriers of two variant alleles, as compared with carriers of the common (wild-type) allele. In multivariate analysis, the increase in intima-media thickness among carriers of two variant alleles (62 microm, P<0.001) was similar in this cohort to that associated with diabetes (64 microm, P=0.01), the strongest common cardiovascular risk factor. Increased dietary arachidonic acid significantly enhanced the apparent atherogenic effect of genotype, whereas increased dietary intake of n-3 fatty acids blunted the effect. Finally, the plasma level of C-reactive protein, a marker of inflammation, was increased by a factor of 2 among carriers of two variant alleles as compared with that among carriers of the common allele.


CONCLUSIONS
Variant 5-lipoxygenase genotypes identify a subpopulation with increased atherosclerosis. The observed diet-gene interactions further suggest that dietary n-6 polyunsaturated fatty acids promote, whereas marine n-3 fatty acids inhibit, leukotriene-mediated inflammation that leads to atherosclerosis in this subpopulation.
Dwyer
James H
JH
Department of Preventive Medicine, Keck School of Medicine, University of Southern California, CA 91803-8000, USA. jimdwye@usc.edu
Allayee
Hooman
H
Dwyer
Kathleen M
KM
Fan
Jing
J
Wu
Huiyun
H
Mar
Rebecca
R
Lusis
Aldons J
AJ
Mehrabian
Margarete
M
D000328:Adult; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D001161:Arteriosclerosis / Q000097:blood / Q000235:genetics* / Q000473:pathology; D002339:Carotid Arteries / Q000033:anatomy & histology; D008078:Cholesterol, LDL / Q000097:blood; D004032:Diet; D005227:Fatty Acids / Q000097:blood / Q000494:pharmacology; D005260:Female; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000235:genetics; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D012307:Risk Factors; D017539:Tunica Intima / Q000033:anatomy & histology; D017540:Tunica Media / Q000033:anatomy & histology
D008078:Cholesterol, LDL; D005227:Fatty Acids; D001094:Arachidonate 5-Lipoxygenase