Visual detection of cysteine and homocysteine.
The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal.
Rusin
Oleksandr
O
Department of Chemistry and Center for Advanced Microstructures and Devices, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
St  Luce
Nadia N
NN
Agbaria
Rezik A
RA
Escobedo
Jorge O
JO
Jiang
Shan
S
Warner
Isiah M
IM
Dawan
Fareed B
FB
Lian
Kun
K
Strongin
Robert M
RM
D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D003545:Cysteine / Q000032:analysis* / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D006710:Homocysteine / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014966:Xanthenes / Q000737:chemistry
D004396:Coloring Agents; D014966:Xanthenes; D006710:Homocysteine; D003545:Cysteine