Genetic instability in EBV-transformed lymphoblastoid cell lines.
Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.
Mohyuddin
Aisha
A
Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories, G.P.O. Box 2891, Islamabad 44000, Pakistan.
Ayub
Qasim
Q
Siddiqi
Saima
S
Carvalho-Silva
Denise R
DR
Mazhar
Kehkashan
K
Rehman
Sadia
S
Firasat
Sadaf
S
Dar
Ahsana
A
Tyler-Smith
Chris
C
Mehdi
S Qasim
SQ
D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D041322:Chromosomes, Human, Y; D004261:DNA Replication; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation