Establishment of OC3 oral carcinoma cell line and identification of NF-kappa B activation responses to areca nut extract.
BACKGROUND
Cell lines derived from oral squamous cell carcinoma (OSCC) exposed to variable etiological factors can bestow advantages in understanding the molecular and cellular alterations pertaining to environmental impacts. Most OSCC cell lines have been established from smoker patients or areca chewing/smoker patients, carrying the genomic alterations in p53.


METHODS
A new cell line, oral carcinoma 3 (OC3), was established from an OSCC in a long-term areca (betel) chewer who does not smoke. Cellular and molecular features of OC3 were determined by variable assays.


RESULTS
The cultured monolayer cells were mainly polygonal and had the expression of cytokeratin 14. The chromosomal analysis using comparative genomic hybridization has revealed the gain in chromosomes 1q, 5q, and 8q, the loss in 4q, 6p, and 8p as well as the gain of entire chromosome 20. Loss of heterozygosity and instability in multiple microsatellite markers in chromosome 4q were also noted. OC3 cells bear wild-type p53 coding sequence and have a high level of p53 expression. Its p21 expression was similar to that in normal human oral keratinocyte (NHOK). Interestingly, activation of nuclear factor kappa B (NF-kappa B) in OC3 cells following the treatment of areca nut extract was observed.


CONCLUSIONS
OC3 cell line could be valuable in understanding the genetic impairments and phenotypic changes associated with areca in oral keratinocyte.
Lin
Shu-Chun
SC
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan. sclin@ym.edu.tw
Liu
Chung-Ji
CJ
Chiu
Chun-Po
CP
Chang
Shun-Ming
SM
Lu
Suu-Yi
SY
Chen
Yann-Jang
YJ
D001114:Areca / Q000009:adverse effects*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D002869:Chromosome Aberrations; D002894:Chromosomes, Human, Pair 4 / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000633:toxicity; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D016328:NF-kappa B; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53