The hypothalamus is involved in the anorexic effect of glucagon-like peptide-1 in chicks.
The present study was carried out to investigate whether the hypothalamus is involved in the anorexic effect of glucagon-like peptide-1 (GLP-1) in chicks. To examine this, Fos expression in the chick hypothalamus were immunohistochemically detected after intracerebroventricular (ICV) injection of 30-pmol GLP-1. ICV injection of GLP-1 stimulated the expression of Fos-like immunoreactive (FLI) cells in the ventromedial hypothalamic nucleus (VMN). When 15-pmol GLP-1 was directly injected into the chick VMN, the chick's food intake was significantly decreased compared with the control treatment. Microinjection of GLP-1 into the (LHA) also inhibited feeding in chicks, although ICV injection of GLP-1 did not stimulate FLI expression in the brain area. These results suggest that VMN and some brain regions are involved in the anorexic effect of GLP-1 in chicks.
Tachibana
Tetsuya
T
United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. tetsu@brs.kyushu-u.ac.jp
Hirofuji
Keiko
K
Matsumoto
Mako
M
Furuse
Mitsuhiro
M
Hasegawa
Shin
S
Yoshizawa
Fumiaki
F
Sugahara
Kunio
K
D000818:Animals; D000855:Anorexia / Q000503:physiopathology*; D002645:Chickens; D005247:Feeding Behavior / Q000502:physiology; D005934:Glucagon / Q000008:administration & dosage / Q000502:physiology*; D052216:Glucagon-Like Peptide 1; D007031:Hypothalamus / Q000166:cytology / Q000502:physiology*; D007276:Injections, Intraventricular; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000502:physiology*; D011498:Protein Precursors / Q000008:administration & dosage / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D014697:Ventromedial Hypothalamic Nucleus / Q000378:metabolism
D010446:Peptide Fragments; D011498:Protein Precursors; D016760:Proto-Oncogene Proteins c-fos; D052216:Glucagon-Like Peptide 1; D005934:Glucagon