[Inter-laboratory evaluation studies of notified ELISA methods for allergic substances (egg)].
Inter-laboratory evaluation studies were conducted for the notified ELISA methods for allergic substances (Egg). Standard extracts of egg spiked in extracts of sausage, sauce, cookie, bread and cereal at a level of 5-20 ng/mL as the sample solution were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of all three ELISA methods using an Egg Protein ovalbumin ELISA Kit (ovalbumin kit), an Egg Protein ovomucoid ELISA Kit (ovomucoid kit) and a FASTKIT Egg ELISA kit (Egg ELISA kit) were mostly less than 10%. Mean recoveries of the standard extract of egg were over 40% in the three ELISA methods. Repeatability relative standard deviations of egg standard solution in five food extracts were in the ranges of 18.7-25.5%, 18.6-41.8%, 21.3-43.3% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. Reproducibility relative standard deviations of egg standard solution in five food extracts were 16.8-35.1%, 19.6-35.8%, 24.7-51.1% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. The detection limits of all the ELISA methods were 4-5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of egg protein levels in extracts of sausage, sauce, cookie, bread and cereal.
Akiyama
Hiroshi
H
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Isuzugawa
Kazuto
K
Harikai
Naoki
N
Watanabe
Hiroko
H
Iijima
Ken
K
Yamakawa
Hirohito
H
Mizuguchi
Yamato
Y
Yoshikawa
Reiji
R
Yamamoto
Miho
M
Sato
Hidetaka
H
Watai
Masatoshi
M
Arakawa
Fumihiro
F
Ogasawara
Takeshi
T
Nishihara
Rikuka
R
Kato
Hisashi
H
Yamauchi
Atsushi
A
Takahata
Yoshihisa
Y
Morimatsu
Fumiki
F
Mamegoshi
Shinichi
S
Muraoka
Shiroo
S
Honjoh
Tsutomu
T
Watanabe
Takahiro
T
Wakui
Chiseko
C
Imamura
Tomoaki
T
Toyoda
Masatake
M
Maitani
Tamio
T
D000485:Allergens / Q000032:analysis*; D004527:Egg Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005504:Food Analysis / Q000592:standards*; D015203:Reproducibility of Results
D000485:Allergens; D004527:Egg Proteins