title: Principles and methods of affinity cleavage in studying transcription.
authors.affiliation: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, California 95616-5295, USA.
authors.forename: Claude F
authors.initials: CF
authors.lastname: Meares
authors.forename: Saul A
authors.initials: SA
authors.lastname: Datwyler
authors.forename: Brian D
authors.initials: BD
authors.lastname: Schmidt
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Owens
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D001671:Biochemistry / Q000379:methods*; D015153:Blotting, Western; D002614:Chelating Agents / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007501:Iron / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D013824:Thermus / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D002614:Chelating Agents; D003432:Cross-Linking Reagents; D007477:Ions; D010455:Peptides; D011108:Polymers; C042007:1-(4-bromoacetamidobenzyl)EDTA; D004247:DNA; D004492:Edetic Acid; D007501:Iron; D012321:DNA-Directed RNA Polymerases; D003545:Cysteine