Identification of low-dye-binding (ldb) mutants of Saccharomyces cerevisiae.
We have completed the identification of Saccharomyces cerevisiae genes that are defective in previously isolated ldb (low-dye-binding) mutants. This was done by complementation of the mutant's phenotype with DNA fragments from a genomic library and by running standard tests of allelism with single-gene deletion mutants of similar phenotype. The results were as follows: LDB2 is allelic to ERD1; LDB4 to SPC72; LDB5 to RLR1; LDB6 to GON7/YJL184W; LDB7 to YBL006C; LDB9 to ELM1; LDB10 to CWH36; LDB11 to COG1; LDB12 to OCH1; LDB13 to VAN1; LDB14 to BUD32; and LDB15 to PHO85. Since the precise function of some of the genes is not known, these data may contribute to the functional characterization of the S. cerevisiae genome.
Corbacho
Isaac
I
Department of Microbiology, University of Extremadura, 06071 Badajoz, Spain.
Olivero
Isabel
I
Hernández
Luis M
LM
D000483:Alleles; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D016681:Genome, Fungal; D015698:Genomic Library; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013172:Spores, Fungal
D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins