[The distribution of calmodulin with midbody and its involvement in cytokinesis regulation].
Calmodulin (CaM) is a major cytoplasmic Ca2+ receptor and performs a multiplicity of functions in the cell. By using GFP-CaM fusion protein, we have studied the detailed dynamic redistribution of CaM during cytokinesis in HeLa cells. CaM associates with midbody in late cytokinesis phase. When the cells were treated with Ca2+/CaM inhibitor W7, the dissolving of the midbody was delayed. Moreover, we have found that gamma-tubulin colocalized with CaM at the midbody during cytokinesis. W7 treatment could affect the dissociation of gamma-tubulin from midbody. These results suggest that CaM may involve in the regulation of midbody microtubules disassembly and may thus affect the completion of cytokinesis.
Yu
Yue Yue
YY
Life Science School of Nanjing Normal University, Nanjing 210097.
Dai
Gu
G
Chen
Jie
J
Wen
Chuan Jun
CJ
Zhao
Dong Hong
DH
Li
Chao Jun
CJ
D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002453:Cell Cycle; D002455:Cell Division / Q000502:physiology; D018385:Centrosome / Q000378:metabolism / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
D002147:Calmodulin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D014404:Tubulin; D049452:Green Fluorescent Proteins; C017967:W 7