title: [Preparation of the microarray for detecting hepatitis D virus].
abstract: OBJECTIVE
  To prepare the microarray for detection of hepatitis D virus (HDV).
  
  
  METHODS
  Several pairs of specific PCR primers were designed according to the conserved region of HDV genome. The DNA microarray were prepared by blotting the PCR products onto the surface of glass slides with the use of robotics, and restriction display PCR (RD-PCR) was employed to label the samples.
  
  
  RESULTS
  Sequences analysis showed that the products of PCR amplification were the specific gene fragments of HDV. Hybridization signals on the gene chip demonstrated good specificity and sensitivity of the microarray for HDV detection.
  
  
  CONCLUSIONS
  Microarray-based clinical HDV detection can be sensitive and effective.
authors.affiliation: Institute of Molecular Oncology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Sun
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood
chemical_list: D012367:RNA, Viral