Transcription factors and nuclear receptors interact with the SWI/SNF complex through the BAF60c subunit.
Transcriptional activity relies on coregulators that modify chromatin structure or serve as bridging factors between transcription factors and the basal transcription machinery. We identified a new coregulator of peroxisome proliferator-activated receptor gamma, BRG1/Brm-associated factor of 60 kDa, subunit c2 (BAF60c2), in a yeast two-hybrid screen of a human adipose tissue cDNA library. BAF60c2 represents a new isoform of BAF60c, a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex. This new isoform as well as the previously identified protein, renamed BAF60c1, is localized primarily in the cell nucleus and is expressed in a wide variety of tissues. Both BAF60c isoforms bind to several nuclear receptors and transcription factors of various families. BAF60c proteins interact in a ligand-independent manner with peroxisome proliferator-activated receptor gamma and enhance its transcriptional activity. Both isoforms are enriched in the central nervous system and also modulate the transcriptional activity of retinoic acid-related orphan receptor alpha1. In conclusion, BAF60c represents a new coregulator that constitutes an important anchoring point by which the SWI/SNF complex is recruited to nuclear receptors and other transcription factors.
Debril
Marie-Bernard
MB
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP10142, 67404 Illkirch cedex, France.
Gelman
Laurent
L
Fayard
Elisabeth
E
Annicotte
Jean-Sébastien
JS
Rocchi
Stéphane
S
Auwerx
Johan
J
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002868:Chromosomal Proteins, Non-Histone; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D021122:Protein Subunits; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D021122:Protein Subunits; D018160:Receptors, Cytoplasmic and Nuclear; C109786:SMARCD1 protein, human; C491649:SMARCD3 protein, human; D014157:Transcription Factors