title: Determination of binary pesticide mixtures by an acetylcholinesterase-choline oxidase biosensor.
abstract: In this study, acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on poly(2-hydroxyethyl methacrylate) (pHEMA) membranes to construct a biosensor for the detection of anti-cholinesterase compounds. pHEMA membranes were prepared with the addition of SnCl(4) to achieve the desired porosity. Immobilization of the enzymes was done by surface attachment via epichlorohydrin (Epi) and Cibacron Blue F3G-A (CB) activation. Enzyme immobilized membrane was used in the detection of anti-cholinesterase activity of aldicarb (AS), carbofuran (CF) and carbaryl (CL), as well as two mixtures, (AS+CF) and (AS+CL). The total anti-cholinesterase activity of binary pesticide mixtures was found to be lower than the sum of the individual inhibition values.
authors.affiliation: Departments of Biological Sciences and Biotechnology, Middle East Technical University, 06531, Ankara, Turkey. koek@mpip-mainz.mpg.de
authors.forename: Fatma N
authors.initials: FN
authors.lastname: Kok
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D000448:Aldicarb / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D012721:Carbaryl / Q000032:analysis; D002235:Carbofuran / Q000032:analysis; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D045424:Complex Mixtures / Q000032:analysis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004791:Enzyme Inhibitors / Q000032:analysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D020099:Coated Materials, Biocompatible; D045424:Complex Mixtures; D004791:Enzyme Inhibitors; D010575:Pesticides; D000448:Aldicarb; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; D012721:Carbaryl; D002235:Carbofuran