title: Expression of aquaporins in the cochlea and endolymphatic sac of guinea pig.
abstract: It has been shown that the aquaporin (AQP) family forms membrane pores selectively permeable for water and some small solutes such as glycerol and urea, and thus plays important roles in regulating the fluid in many organs involved in fluid transport such as kidney, lung and brain. The aims of the present study were to investigate the cellular localization and its significance of aquaporins (AQPs) in various subregions of the cochlea and endolymphatic sac of guinea pig. The expression patterns of AQP1, 2, 3 and 4 were immunolabeled with the specific polyclonal rabbit antibodies against the rat AQP1, 2, 3 and 4. Our immunohistochemical examination showed that in the cochlea, AQP1, 3 and 4 were widely distributed in various locations including stria vascularis, spiral ligament, the organ of Corti and spiral ganglion in the similar patterns except that AQP3 in the stria vascularis was lightly weaker than AQP1 and AQP4. AQP2 was labeled only in Reissner's membrane. In the endolymphatic sac, AQP1, AQP3 and AQP4 were strongly expressed in the epithelial cells and subepithelial cells similarly with the exception that AQP3 was lightly weaker than AQP1 and AQP4. No AQP2 immunoreactivity was detected in the endolymphatic sac. Theses results suggest that different members of the AQP family in the labyrinth may work in concert to regulate endolymph and to maintain homeostasis in the inner ear.
authors.affiliation: Department of Otolaryngology and Head-Neck Surgery, Daping Hospital, Third Military Medical University, Chongqing, PR China. zhongsx@online.cq.cn
authors.forename: Shi-Xun
authors.initials: SX
authors.lastname: Zhong
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000378:metabolism*; D003051:Cochlea / Q000378:metabolism*; D004712:Endolymphatic Sac / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D015221:Potassium Channels / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism
chemical_list: D020346:Aquaporins; D015221:Potassium Channels; D015222:Sodium Channels