Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells.
We have recently described a novel, stable human immunodeficiency virus type 1 (HIV-1) vector packaging system, STAR. High-titre HIV-1 vectors bearing gammaretrovirus envelopes (Env) are continuously produced from STAR cells. Here we compare the properties of such vectors, with the amphotropic murine leukaemia virus (MLV-A) Env, a modified gibbon ape leukaemia virus (GALV) Env and two modified versions of the cat endogenous retrovirus RD114 Env, produced from STAR cells, to transiently produced HIV-1 vectors with vesicular stomatitis virus G protein (VSV-G). Our results indicate that gammaretrovirus pseudotypes from STAR cells are relatively stable at 37 degrees C and are resistant to inactivation by freeze/thaw cycling or incubation with human sera. HIV-1(VSV-G) was, however, sensitive to freeze/thaw when harvested in serum-free media and was readily inactivated in human sera. Furthermore, the titre of 'gamma-retrovirus' pseudotypes, but not HIV-1(VSV-G), could be increased by the use of a combination of polybrene and spinoculation. All pseudotypes could be efficiently concentrated, but soluble gammaretrovirus Env could act as an inhibitor of infection.
Strang
B L
BL
Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London, London, UK.
Ikeda
Y
Y
Cosset
F-L
FL
Collins
M K L
MK
Takeuchi
Y
Y
D019149:Bioreactors; D001709:Biotechnology; D002460:Cell Line; D002498:Centrifugation; D003080:Cold Temperature; D017988:Gammaretrovirus / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006583:Hexadimethrine Bromide; D006358:Hot Temperature; D006801:Humans; D007239:Infections / Q000628:therapy; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; D006583:Hexadimethrine Bromide