Guar seed beta-mannan synthase is a member of the cellulose synthase super gene family.
Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (ManS), that makes the beta-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase-like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in beta-glycan formation in plants.
Dhugga
Kanwarpal S
KS
Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, 7300 NW 62nd Avenue, Johnston, IA 50131, USA. Kanwarpal.Dhugga@Pioneer.Com
Barreiro
Roberto
R
Whitten
Brad
B
Stecca
Kevin
K
Hazebroek
Jan
J
Randhawa
Gursharn S
GS
Dolan
Maureen
M
Kinney
Anthony J
AJ
Tomes
Dwight
D
Nichols
Scott
S
Anderson
Paul
P
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D020134:Catalytic Domain; D002482:Cellulose / Q000096:biosynthesis; D031293:Cyamopsis / Q000201:enzymology* / Q000235:genetics; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D005690:Galactose / Q000031:analogs & derivatives; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D008351:Mannans / Q000096:biosynthesis* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000201:enzymology*; D013025:Glycine max / Q000235:genetics; D014170:Transformation, Genetic
D008351:Mannans; C081692:beta-1,4-mannan; C012990:galactomannan; D002482:Cellulose; D005964:Glucosyltransferases; D008364:Mannosyltransferases; C021542:mannan synthetase; D008358:Mannose; D005690:Galactose