title: Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei.
abstract: The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02) without alteration of its specificity.
authors.affiliation: Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de médecine, Université de la Méditerranée, 13385 Marseille cedex 05, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Fenollar
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000192:Actinomycetales / Q000235:genetics / Q000302:isolation & purification*; D016680:Genome, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D008061:Whipple Disease / Q000382:microbiology*