title: Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin.
abstract: We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expression analyses show that the hht2-11 mutation confers pleiotropic transcriptional defects and that many of the genes it affects are normally controlled by the Swi/Snf chromatin remodeling complex. Furthermore, we show that Swi/Snf occupancy at two promoters, PHO84 and SER3, is reduced in hht2-11 mutants. Detailed studies of the PHO84 promoter suggest that the hht2-11 mutation impairs Swi/Snf association with chromatin in a direct fashion. Taken together, our results strongly suggest that the integrity of the globular domain of histone H3 is an important determinant in the ability of Swi/Snf to associate with chromatin.
authors.affiliation: Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Duina
authors.forename: Fred
authors.initials: F
authors.lastname: Winston
mesh_terms: D019943:Amino Acid Substitution; D001483:Base Sequence; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007295:Inositol Phosphates / Q000096:biosynthesis; D046911:Macromolecular Substances; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D006657:Histones; D007295:Inositol Phosphates; D046911:Macromolecular Substances; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators