title: Comparison of sampling techniques for detection of Arcobacter butzleri from chickens.
abstract: Arcobacter butzleri is a causative agent of human enteritis that has been recently differentiated from the genus Campylobacter. Previous work suggests that its transmission to humans is likely through a foodborne route with a substantial tendency to be located on poultry carcasses. For reducing the incidence of this pathogen on commercial poultry, improved protocols are needed to sample and identify A. butzleri from infected birds prior to slaughter. The purpose of this study was to compare sampling methods for this emerging pathogen from chickens that were artificially inoculated per os with A. butzleri. We tested three sampling techniques commonly used to determine the microbiological quality of poultry: cloacal swabs, fecal samples, and environmental surface (drag) swabs collected when birds were 3, 5, or 7 wk old. These samples were cultured in Johnson-Murano enrichment broth and analyzed by PCR. Results indicate that environmental surface swabs yielded the highest recovery percentage. A detection rate of 75 to 100% was observed for each sampling period (age of chicken). Additionally, A. butzleri could not be isolated from the intestinal tract (jejunum, ileum, cecum, colorectum) of inoculated birds.
authors.affiliation: Department of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061, USA. jeifert@vt.edu
authors.forename: J D
authors.initials: JD
authors.lastname: Eifert
authors.forename: R M
authors.initials: RM
authors.lastname: Castle
authors.forename: F W
authors.initials: FW
authors.lastname: Pierson
authors.forename: C T
authors.initials: CT
authors.lastname: Larsen
authors.forename: C R
authors.initials: CR
authors.lastname: Hackney
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D002988:Cloaca / Q000382:microbiology; D004777:Environment; D005243:Feces / Q000382:microbiology; D006799:Housing, Animal; D007422:Intestines / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*