Cryopreservation of the unicellular marine alga, Nannochloropsis oculata.
In microalgal culture collections, as in many biological resource centres, cryoconservation is the most attractive method for the long-term, secure storage of living material. Nannochloropsis oculata, a marine unicellular alga, is of interest in the field of biotechnology due to its high lipid content. Of various cryoprotectants tested for their toxicity and for their ability to prevent cryoinjury, glycerol (final concentration 1.1 M) was the most efficient. When glycerol-treated cultures were submitted to a strictly regulated cooling rate (-3 degrees C min(-1)), they attained the control culture density within 13 d after thawing.
Poncet
Jean-Marc
JM
Laboratoire de Biologie et Biotechnologies Marines, Université de Caen Basse-Normandie, BP 5186, 14032 Caen, France. jm_poncet@yahoo.com
Véron
Benoît
B
D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D056890:Eukaryota / Q000145:classification / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D005990:Glycerol / Q000494:pharmacology; D008386:Marine Biology / Q000379:methods*; D000432:Methanol / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology
D003451:Cryoprotective Agents; D003470:Culture Media; D011392:Proline; D005990:Glycerol; D000432:Methanol; D004121:Dimethyl Sulfoxide