Evolutionary characteristics of HIV type 1 variants resistant to protease inhibitors in the absence of drug-selective pressure.
To understand the evolutionary characteristics of HIV-1 variants resistant to protease inhibitors (PI), the replicating plasma virus was analyzed in three patients shifted to PI-sparing regimen after virological failure. The dynamic features of carryover mutations associated with PI resistance in the absence of selective pressure on the protease gene indicate that viral variants resistant to reverse transcriptase inhibitors and bearing mutations of the protease sequence can maintain efficient replication capacity in vivo.
Boeri
Enzo
E
Diagnostica and Ricerca San Raffaele, Laoratorio di Virologia, Centro San Luigi, I.R.C.C.S. Istituto Scientifico San Raffaele, 20 20127 Milan, Italy. enzo.boer@hsr.it
Gianotti
Nicola
N
Canducci
Filippo
F
Hasson
Hamid
H
Giudici
Barbara
B
Castagna
Antonella
A
Lazzarin
Adriano
A
Clementi
Massimo
M
D024882:Drug Resistance, Viral / Q000235:genetics*; D019143:Evolution, Molecular; D016333:HIV Protease / Q000235:genetics*; D017320:HIV Protease Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D010802:Phylogeny
D017320:HIV Protease Inhibitors; D016333:HIV Protease