[Changes of hypothalamus and peripheral orphanin in fetal rats with intrauterine ischemia and hypoxia].
OBJECTIVE
To evaluate the role of orphanin in the perinatal ischemia-hypoxia.


METHODS
The concentration of hypothalamus and peripheral orphanin were measured by radioimmunoassay. Animal model of perinatal ischemia-hypoxia was set up by ligating uterine vessels. All the rats were delivered by cesarean section and scored according to respiration, heart beat, skin color, muscle tone and reflex after delivery.


RESULTS
(1) The levels of orphanin in hypothalamus and peripheral blood in group B were (114 +/- 21) pg/g and (58 +/- 11) ng/L respectively. In group A, the were (71 +/- 14) pg/g and (31 +/- 7) ng/L respectively, the levels of orphanin in group B increased significantly when compared with the group A (P < 0.05). In control group, the levels of orphanin were (48 +/- 9) pg/g and (19 +/- 4) ng/L. The levels of orphanin in group A and B were significantly higher than those in the control group (P < 0.01, P < 0.05). (2) The Apgar scores in groups A and B were significantly decreased than that in control group (P < 0.01). The group A pups had significantly better scores than the group B (P < 0.05).


CONCLUSIONS
The perinatal ischemia and hypoxia was associated with orphanin, the changes of orphanin levels may play an important role in the pathophysiological changes in perinatal ischemia and hypoxia.
Gu
Hang
H
Department of Obstetrics and Gynecology, Affiliated Changhai Hospital of the Second Military Medical University, Shanghai 200433, China.
Hu
Dian
D
Hong
Xin-ru
XR
Li
Wei
W
Cui
Ying
Y
Hui
Ning
N
Sha
Jin-yan
JY
D000818:Animals; D000831:Animals, Newborn; D001034:Apgar Score; D005260:Female; D007031:Hypothalamus / Q000737:chemistry*; D000860:Hypoxia / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D018847:Opioid Peptides / Q000032:analysis* / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000098:blood supply*; D000097629:Nociceptin
D018847:Opioid Peptides; D000097629:Nociceptin