[Effect of fructose-1,6-diphosphate and dexamethasone on ischemia/reperfusion injury after hemorrhagic shock in rabbits].
OBJECTIVE
To investigate the mechanism of myocardial ischemia/reperfusion injury and the protective effect of fructose-1, 6-diphosphagic (FDP) and dexamethasone (DXM) in hemorrhagic shock in rabbits.


METHODS
Using a hemorrhagic shock model of Wiggers, 48 rabbits were randomly divided into 6 groups. Group I control group; GroupII with drugs given before ischemia phase (divided into 3 groups: FDP I, DXM I and FDP I+ DXM I); Group III with drugs given in reperfusion phase (divided into 2 groups: FDPII and DXMII). The levels of creatine kinase (CK) and troponin I (cTnI) in plasma were measured, and myocyte apoptosis index was assessed.


RESULTS
Baseline levels of CK and cTnI were similar in three groups; CK and cTnI and apoptosis index were lower or with a lower tendency in groupII and in groupIII (P<0.05 or P<0.01); CK and cTnI showed a lower tendency in rise in FDP I and DXM I than in FDPII and even slower in FDP group than in DXM group; CK and cTnI levels rose slower in FDP I+DXM I than in FDP I and DXM I.


CONCLUSIONS
FDP given during ischemia and DXM could effectively protect the myocardium from reperfusion injury following hemorrhagic shock.
Chen
Yong-quan
YQ
Yijishan Hospital, College of Wannan Medical, Wuhu 241001, Anhui, China.
Jin
Xiao-ju
XJ
Tang
Rong-hua
RH
Huang
Wen-bin
WB
Tao
Qing-song
QS
Wang
Bang-an
BA
D000818:Animals; D002317:Cardiovascular Agents / Q000627:therapeutic use; D003402:Creatine Kinase / Q000097:blood; D003907:Dexamethasone / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005260:Female; D005635:Fructosediphosphates / Q000627:therapeutic use*; D005938:Glucocorticoids / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D011817:Rabbits; D011897:Random Allocation; D012771:Shock, Hemorrhagic / Q000150:complications*; D019210:Troponin I / Q000097:blood
D002317:Cardiovascular Agents; D005635:Fructosediphosphates; D005938:Glucocorticoids; D019210:Troponin I; D003907:Dexamethasone; D003402:Creatine Kinase; C029063:fructose-1,6-diphosphate