title: Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column.
abstract: A rapid HPLC-DAD determination of phenols in apple using an RP monolithic column is reported. Because of the hydrodynamic advantages offered by this kind of column and the use of acidified acetonitrile as eluent, assays of apple extracts can be performed in <21 min. Assays of pulp and peel extracts were carried out without the need for time-consuming sample pretreatment except filtration. Several flavanols, hydroxycinnamic acids, dihydrochalcones, and six quercetin glycosides were identified and quantified. A seventh quercetin derivative, two chalcone-related compounds, and three hydroxycinnamic derivatives were also found. Peels proved to be richer in phenols than pulps, the former being composed mainly of (-)-epicatechin, procyanidin B2, chlorogenic acid, phloridzin, hyperin, and avicularin. In pulps, where the chlorogenic acid was the principal phenolic compound, quercetin glycosides were found in very low amounts.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università di Bologna, 40127 Bologna, Italy. chinnici@agrsci.unibo.it
authors.forename: Fabio
authors.initials: F
authors.lastname: Chinnici
authors.forename: Anna
authors.initials: A
authors.lastname: Gaiani
authors.forename: Nadia
authors.initials: N
authors.lastname: Natali
authors.forename: Claudio
authors.initials: C
authors.lastname: Riponi
authors.forename: Sergio
authors.initials: S
authors.lastname: Galassi
mesh_terms: D000097:Acetonitriles; D044946:Biflavonoids; D002392:Catechin; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D044945:Proanthocyanidins
chemical_list: D000097:Acetonitriles; D044946:Biflavonoids; D044948:Flavonols; D010636:Phenols; D044945:Proanthocyanidins; C479580:procyanidin B2; D002392:Catechin; C032159:acetonitrile