High resolution HLA-DRB1 identification of a Caucasian population.
Polymerase chain reaction-sequence-specific oligonucleotide probes typing methods have been applied to 1000 individuals from the Northern Ireland population to give human leukocyte antigen DRB1 (HLA-DRB1) allele assignment. HLA-DRB1 allele frequencies and four-locus haplotypes (A/B/C/DR) for this Caucasian population, based on HLA class I and class II allele assignment, are now presented. No significant deviations from Hardy-Weinberg proportions were observed. The HLA-C locus exhibited marginal evidence of selection (p<0.03, uncorrected one-sided test) in the direction of balancing selection; the HLA-A, -B, and -DRB1 allele frequency distributions were compatible with expectations under a neutral model (which does not mean that selection is not operating). Evidence for selection was seen on haplotypes HLA-A*010101-B*0801-DRB1*030101 and HLA-A*290201-B*440301-DRB1*070101 based on their patterns of linkage disequilibrium.
Williams
Fionnuala
F
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland. fionnuala.williams@bll.n-i.nhs.uk
Meenagh
Ashley
A
Single
Rich
R
McNally
Mark
M
Kelly
Philip
P
Nelson
Mark P
MP
Meyer
Diogo
D
Lancaster
Alex
A
Thomson
Glenys
G
Middleton
Derek
D
D000483:Alleles; D003062:Codon / Q000235:genetics; D004739:England / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D015810:Linkage Disequilibrium; D008163:Luminescent Measurements; D008957:Models, Genetic; D009660:Northern Ireland; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012606:Scotland / Q000208:ethnology; D044465:White People / Q000235:genetics*
D003062:Codon; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015345:Oligonucleotide Probes