[Clinical significance and correlation between elevated serum TR6 and lympho-metastasis in gastric cancer].
OBJECTIVE
To evaluate the value of serum TR(6) for the diagnosis and TNM classification in patients with gastric carcinoma.


METHODS
Serum TR(6) levels were measured using ELISA method in 31 gastric cancer patients, 19 patients with nonmalignant conditions and 29 healthy individuals. TR(6) expression in tumor mass was studied with immunohistochemistry. TR(6) gene copy number in tumor tissues was evaluated by real time PCR.


RESULTS
Ninety-seven point nine percent (47 of 48 cases) of healthy individuals and patients with nonmalignant conditions were serum TR(6)-negative. In contrast, 71% (22 of 31 cases) of gastric cancer patients were serum TR(6)-positive. Serum TR(6) positiveness was closely correlated with tumor differentiation status and TNM classification. TR(6) gene amplification did not occur in gastric carcinoma.


CONCLUSIONS
Serum TR(6) levels were correlated significantly with TNM stage and histopathological type of tumor. This can help to determine the pre-operative TNM classification and to choose the optimal extent of lymph node dissection for gastric cancer.
Wu
Yu-lian
YL
Department of Surgery, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Yu
Jun-xiu
JX
Shen
Hong-wei
HW
Han
Bing
B
Gao
Shun-liang
SL
D006801:Humans; D008207:Lymphatic Metastasis; D008562:Membrane Glycoproteins / Q000097:blood*; D009367:Neoplasm Staging; D011956:Receptors, Cell Surface / Q000097:blood*; D018124:Receptors, Tumor Necrosis Factor; D053323:Receptors, Tumor Necrosis Factor, Member 6b; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology
D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D053323:Receptors, Tumor Necrosis Factor, Member 6b; C505911:TNFRSF6B protein, human