title: Diagnosis of intra-oral MALT lymphoma using seminested polymerase chain reaction.
abstract: Diagnosis of mucosa-associated lymphoid tissue (MALT) lymphoma based on histological examination alone is difficult. We report three patients with histologically suspected MALT lymphoma who developed lymphoproliferative lesions of the sublingual gland. Seminested polymerase chain reaction (PCR) analysis applied to formalin-fixed and paraffin-embedded specimens showed clonal rearrangement of immunoglobulin heavy chain genes in two patients and a polyclonal characteristic in one. The clinical findings and Southern blot analysis confirmed the accuracy of the diagnosis. The molecular method described can be applied routinely to processed specimens to obtain helpful information for the diagnosis of low-grade malignancies of lymphoproliferative disorders, such as MALT lymphoma.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Medical University Kasumigaura Hospital, 3-20-1 Chuo Ami-machi, Inashiki-gun, Ibaraki 300-0395, Japan. khonda@tokyo-med.ac.jp
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Honda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kusama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takagi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sekine
authors.forename: Masayuki
authors.initials: M
authors.lastname: Noguchi
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Chiba
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology; D002999:Clone Cells / Q000473:pathology; D005260:Female; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis*; D008875:Middle Aged; D009060:Mouth Floor / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D013362:Sublingual Gland Neoplasms / Q000175:diagnosis; D013365:Submandibular Gland Neoplasms / Q000175:diagnosis
chemical_list: D007143:Immunoglobulin Heavy Chains