title: Effect of fetal macrosomia on human placental glucose transport and utilization in insulin-treated gestational diabetes.
abstract: The aim of this study was to compare glucose transport and utilization in human placentae from pregnancies affected by insulin-treated GDM with and without macrosomia, and from non-diabetic control pregnancies. Placental lobules were perfused for 4 h. Maternal D-glucose concentration was 4, 8, 16, or 24 mM while the fetal D-glucose was maintained at 3mM. 14C-D-glucose and 3H-L-glucose were infused into the maternal circulation. Radioactivity, D-glucose and L-lactate levels were measured in the fetal and maternal effluent perfusates. Glucose uptake from the maternal perfusate, and transfer to the fetal effluent were not significantly different between groups. Insulin-treated GDM group without macrosomia had reduced glucose utilization compared to the control group while the insulin-treated GDM group with macrosomia did not. Lactate release into the fetal effluent was significantly reduced in both insulin-treated GDM groups compared to the control group. In conclusion, placental glucose utilization is different between insulin-treated GDM placentae with and without fetal macrosomia.
authors.affiliation: Department of Pharmacology, Monash University, Clayton, Australia.
authors.forename: Roger G
authors.initials: RG
authors.lastname: King
authors.forename: David T D
authors.initials: DT
authors.lastname: Osmond
authors.forename: Shaun P
authors.initials: SP
authors.lastname: Brennecke
authors.forename: Neil M
authors.initials: NM
authors.lastname: Gude
mesh_terms: D000328:Adult; D001692:Biological Transport; D001786:Blood Glucose / Q000032:analysis; D016640:Diabetes, Gestational / Q000188:drug therapy* / Q000378:metabolism*; D005260:Female; D005320:Fetal Macrosomia / Q000378:metabolism*; D005865:Gestational Age; D005947:Glucose / Q000378:metabolism*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000627:therapeutic use*; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D001786:Blood Glucose; D005947:Glucose; D006442:Glycated Hemoglobin; D007328:Insulin