Cloning cattle.
Over the past six years, hundreds of apparently normal calves have been cloned worldwide from bovine somatic donor cells. However, these surviving animals represent less than 5% of all cloned embryos transferred into recipient cows. Most of the remaining 95% die at various stages of development from a predictable pattern of placental and fetal abnormalities, collectively referred to as the "cloning-syndrome." The low efficiency seriously limits commercial applicability and ethical acceptance of somatic cloning and enforces the development of improved cloning methods. In this paper, we describe our current standard operating procedure (SOP) for cattle cloning using zona-free nuclear transfer. Following this SOP, the output of viable and healthy calves at weaning is about 9% of embryos transferred. Better standardization of cloning protocols across and within research groups is needed to separate technical from biological factors underlying low cloning efficiency.
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Reproductive Technologies, AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand. bjorn.oback@agresearch.co.nz
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D000818:Animals; D001755:Blastocyst / Q000378:metabolism / Q000502:physiology; D002417:Cattle; D002453:Cell Cycle; D002459:Cell Fusion / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D005347:Fibroblasts / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D015044:Zona Pellucida / Q000502:physiology