HuBMSC-MCP, a novel member of mitochondrial carrier superfamily, enhances dendritic cell endocytosis.
A novel member of mitochondrial carrier superfamily has been identified from human bone marrow stromal cells (BMSC) and designated as human BMSC-derived mitochondrial carrier protein (HuBMSC-MCP). It encodes a 321 amino-acid protein with three tandem related domains of about 100 amino acids. Each domain contains two hydrophobic stretches, which are thought to span the membrane as alpha-helices. Distant relationship analysis indicates that the protein is highly conserved between species from Caenorhabditis elegans to human. HuBMSC-MCP gene is mapped to chromosome 11p11. HuBMSC-MCP mRNA expression is detectable in various human tissues and cell lines. By confocal imaging, HuBMSC-MCP is localized to mitochondria and also detected in the pseudopodial protrusion of human breast adenocarcinoma MCF-7 cells. When transfected into dendritic cells (DC), HuBMSC-MCP could enhance DCs endocytotic capacity. Thus, HuBMSC-MCP is a phylogenetically conserved and widely expressed mitochondrial carrier protein which perhaps associates with mitochondrial oxidative phosphorylation.
Wang
Baomei
B
Institute of Immunology, Second Military Medical University, Shanghai, PR China.
Li
Nan
N
Sui
Lili
L
Wu
Yanfeng
Y
Wang
Xiaojian
X
Wang
Qingqing
Q
Xia
Dajing
D
Wan
Tao
T
Cao
Xuetao
X
D000595:Amino Acid Sequence; D001854:Bone Marrow Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000737:chemistry / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism; D009928:Organ Specificity; D017386:Sequence Homology, Amino Acid; D017154:Stromal Cells / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; C483480:SLC25A33 protein, human