Effect of gamma irradiation on toxicity and immunogenicity of Androctonus australis hector venom.
An investigation was made of the radiosensitivity of the toxic and immunological properties of Androctonus australis hector venom. This venom was irradiated with two doses of gamma rays (1 and 2 kGy) from a 60Co source. The results showed that venom toxicity was abolished for the two radiation doses (1 and 2 kGy) with, respectively, 10 and 25 times its initial LD50 value. However, irradiated venoms were immunogenic, and the antibodies elicited by them were able to recognize the native venom by enzyme-linked immunosorbent assay. Antisera raised against these toxoids (1 and 2 kGy) had a higher neutralizing capacity and immunoreactivity against all components of native venom than did the antiserum produced against the native venom. The antiserum of rabbits immunized with 2-kGy-irradiated venom was more efficient than 1-kGy-irradiated toxoid antiserum. Indeed, in vivo protection assays showed that the mice immunized with 2-kGy-irradiated venom resisted lethal doses (i.p.) of A. australis hector venom.
Abib
Lila
L
Laboratoire de Biologie Cellulaire ey Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne Bab Ezzouar, Algérie.
Laraba-Djebari
Fatima
F
D000818:Animals; D000997:Antivenins / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005720:Gamma Rays; D007106:Immune Sera / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D007115:Immunization Schedule; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D011817:Rabbits; D012604:Scorpion Venoms / Q000008:administration & dosage / Q000276:immunology* / Q000528:radiation effects* / Q000633:toxicity; D023382:Toxicity Tests, Acute
D000997:Antivenins; D007106:Immune Sera; D012604:Scorpion Venoms