Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L.
A cell differentiation bioassay measuring alkaline phosphatase activity was developed using osteoblast-like UMR 106 cell line as a model. The effect of fractions and compounds of Psoralea corylifolia L. extract on osteoblastic differentiation was investigated. The fractions or compounds were co-cultured with cells for 48 h, the cellular ALP activity was then measured. The crude ethanol extract of Psoralea corylifolia L. increased ALP activity by 39.5% at a concentration of 0.1 mg/ml, and the activity was concentrated in the ethyl acetate fraction, which produced a maximum ALP activity increase of 38.2%. A significant activity was observed for a flavonoid, corylin, providing 30.0% increment in ALP. These effects suggest that Psoralea corylifolia L. extract, its ethyl acetate fraction and corylin would stimulate bone formation.
Xiong
Z
Z
School of Pharmacy, Shenyang Pharmaceutical University, China.
Wang
D
D
Xu
Y
Y
Li
F
F
D000085:Acetates; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D000431:Ethanol; D005638:Fruit / Q000737:chemistry; D007202:Indicators and Reagents; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D029907:Psoralea / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012997:Solvents
D000085:Acetates; D007202:Indicators and Reagents; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; C007650:ethyl acetate; D000469:Alkaline Phosphatase