title: Repeated electroconvulsive shock treatment increases the expression of A kinase anchoring proteins in the rat hippocampus.
abstract: Electroconvulsive shock (ECS) is widely used in the treatment of psychiatric disorders, but its mode of action remains largely unknown. Thus, this study was performed to examine the effect of repeated ECS treatment on the expression of A kinase anchoring proteins (AKAPs) in the brain. Rats were treated with ECS daily for 10 days. The expression of AKAP protein was analyzed by Western blotting, and AKAP mRNA by real-time quantitative RT-PCR. Repeated ECS treatment for 10 days resulted in increases in the levels of the protein and mRNA of AKAP150, yotiao, and ezrin in the rat hippocampus. Thus, repeated ECS treatment is suggested to increase the reactivity of glutamatergic synapses by increasing the expressions of the AKAPs, which can recruit protein kinase A to glutamate receptors.
authors.affiliation: Department of Biochemistry, Seoul National University College of Medicine, Seoul 110-799, South Korea.
authors.forename: Yun-Il
authors.initials: YI
authors.lastname: Lee
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Koo
authors.forename: Chin-Ho
authors.initials: CH
authors.lastname: Cho
authors.forename: MiRan
authors.initials: M
authors.lastname: Seo
authors.forename: Ung Gu
authors.initials: UG
authors.lastname: Kang
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Juhnn
mesh_terms: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D004597:Electroshock / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006624:Hippocampus / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C415703:Akap5 protein, rat; D002352:Carrier Proteins; D012333:RNA, Messenger