DNA hybridization in nanostructural molecular assemblies enables detection of gene mutations without a fluorescent probe.
We have developed a simple single nucleotide polymorphisms (SNPs) analysis utilizing DNA hybridization in nanostructural molecular assemblies. The novel technique enables the detection of a single-base mismatch in a DNA sequence without a fluorescent probe. This report describes for the first time that DNA hybridization occurs in the nanostructural molecular assemblies (termed reverse micelles) formed in an organic medium. The restricted nanospace in the reverse micelles amplifies the differences in the hybridization rate between mismatched and perfectly matched DNA probes. For a model system, we hybridized a 20-mer based on the p53 gene sequence to 20-mer complementary oligonucleotides with various types of mismatches. Without any DNA labeling or electrochemical apparatus, we successfully detected the various oligonucleotide mismatches by simply measuring the UV absorbance at 260 nm.
Maruyama
Tatsuo
T
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Park
Lian-Chun
LC
Shinohara
Toshimitsu
T
Goto
Masahiro
M
D004252:DNA Mutational Analysis / Q000379:methods*; D006801:Humans; D008823:Micelles; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D015345:Oligonucleotide Probes; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D008823:Micelles; D015345:Oligonucleotide Probes; D012997:Solvents; D016159:Tumor Suppressor Protein p53