title: Molecular cloning and expression of bottlenose dolphin (Tursiops truncatus) interleukin-8.
abstract: The bottlenose dolphin interleukin (IL)-8 cDNA was molecularly cloned. The dolphin IL-8 has an open reading frame of 303-bp encoding 101 amino acids. The homology of the amino acid sequence with that of other species was: sheep, 89.1%; cattle, 88.1%; pig, 85.1%; dog, 85.1%; horse, 79.2%; human, 74.5%; and macaque, 72.3%. The amino acid sequence suggested that dolphin IL-8 was a CXC chemokine. The recombinant dolphin IL-8 protein was recognized with anti-ovine IL-8 monoclonal antibody.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sugisawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Endo
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004289:Dolphins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D016209:Interleukin-8