title: Target-selected mutagenesis of the rat.
abstract: The rat is one of the most extensively studied model organisms, and with its genome being sequenced, tools to manipulate gene function in vivo have become increasingly important. We here report proof of principle for target-selected mutagenesis as a reverse genetic or knockout approach for the rat.
authors.affiliation: Functional Genomics Group, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Bart M G
authors.initials: BM
authors.lastname: Smits
authors.forename: Josine
authors.initials: J
authors.lastname: Mudde
authors.forename: Ronald H A
authors.initials: RH
authors.lastname: Plasterk
authors.forename: Edwin
authors.initials: E
authors.lastname: Cuppen
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis; D005038:Ethylnitrosourea; D005298:Fertility / Q000235:genetics; D005640:Follicle Stimulating Hormone / Q000235:genetics; D018095:Germ-Line Mutation; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar
chemical_list: D009153:Mutagens; D005640:Follicle Stimulating Hormone; D005038:Ethylnitrosourea