[Correlation between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability in rats].
To investigate the relationship between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability of rats.



Using a novel-object recognition model and Morris water maze,the novel-object recognition ability and spatial memory of SD rats were ranked, and grouped as the high (top 20 %) and the low (bottom 20%), respectively. NR1 subunit protein levels in hippocampus were measured by quantitative immunoblotting with NR1 subunit specific antibody.



The level of NR1 subunit protein in hippocampus in the high novel-object recognition ability group was 60% (P<0.01), higher than that in the low one, and in the high spatial memory group it was 45.4 % (P<0.05), higher than that in the low one, respectively.



The basic expression level of NR1 subunit protein in hippocampus is related to novel-object recognition ability and spatial memory of rats.
Xu
Shu-jun
SJ
Department of Neurobiology, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Shen
Hai-qing
HQ
Chen
Zhong
Z
Zhu
Li-jun
LJ
Zhu
Zhao-yang
ZY
Luo
Jian-hong
JH
D000818:Animals; D006624:Hippocampus / Q000737:chemistry* / Q000502:physiology; D015151:Immunoblotting; D007858:Learning; D008297:Male; D018782:Maze Learning; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000502:physiology
C409466:NR1 NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate