title: [Detecting HPV DNA in tissues of external genital squamous cell carcinoma in situ by PCR-RFLP technique].
abstract: OBJECTIVE
  To study the relationship between the infection of human papillomavirus (HPV) and external genital squamous cell carcinoma in situ.
  
  
  METHODS
  HPV DNA was detected with the consensus primer by polymerase chain reaction (PCR) and its type was determined by restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  HPV DNA was detected and HPV16 was the most frequently identified type in 29 (56.9%) of 51 cases of external genital squamous cell carcinoma in situ. HPV DNA was positive in 22 (55%) of 40 cases of bowenoid papulosis, among which 20 were of HPV16 type, one of HPV31 type, and one of HPV6 + 16 type. HPV DNA was positive in all 5 cases (100%) of Bowen's disease, among which 4 were HPV16 type and one of HPV6 + 16 type. HPV DNA was positive in 2 (33.3%) of 6 Queyrat erythroplasia cases and all were of HPV16 type.
  
  
  CONCLUSIONS
  HPV16 infection is strongly associated with the external genital squamous cell carcinoma in situ including bowenoid papulosis, Bowen's disease, and Queyrat erythroplasia.
authors.affiliation: Department of Dermatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. wangjbhe@263.net
authors.forename: Jia-bi
authors.initials: JB
authors.lastname: Wang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Man
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Fang
mesh_terms: D000328:Adult; D000368:Aged; D001913:Bowen's Disease / Q000821:virology; D002278:Carcinoma in Situ / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D004919:Erythroplasia / Q000821:virology; D005260:Female; D005833:Genital Neoplasms, Female / Q000821:virology*; D005834:Genital Neoplasms, Male / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D010412:Penile Neoplasms / Q000821:virology; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections / Q000821:virology*; D014846:Vulvar Neoplasms / Q000821:virology
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral