Maturity of pericytes in cerebral neocapillaries induced by growth factors: fluorescence immuno-histochemical analysis using confocal laser microscopy.
The maturity of pericytes in cerebral neocapillaries induced by two different growth factors: basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF), was examined using an immunohistochemical staining technique. Cerebral angiogenesis was induced in mice by implanting a sandwich system of bFGF/PDGF gel and nylon-mesh over the exposed cortex. On 28th day after incubation, a small volume of cerebral tissue with the nylon-mesh was isolated and stained using tetramethyl rhodamine isothiocyanate (TRITC)-labeled secondary antibody to the primary antibody against NG_2 proteoglycan and fluorescein isothiocyanate (FITC)-labeled Griffonia simplicifolia (GS)-lectin. Using a confocal laser microscopic system, we observed the cerebral neocapillaries on the upper surface of the nylon-mesh and evaluated the maturity of pericytes stained with NG_2 based on the fluorescence immunohistological images. The pericyte appeared rich in neocapillaries induced by PDGF. It was suggested that pericytes might play a key role in the regulation of blood flow in neovessels.
Jariyapongskul
Amporn
A
Department of Vascular Physiology, National Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
Nakano
Atushi
A
Yamaguchi
Saburo
S
Nageswari
Kolammal
K
Niimi
Hideyuki
H
D000818:Animals; D000941:Antigens / Q000032:analysis; D002196:Capillaries / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002540:Cerebral Cortex / Q000098:blood supply* / Q000187:drug effects; D004343:Drug Implants; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D031295:Griffonia; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D020286:Pericytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D037121:Plant Lectins / Q000032:analysis; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D011509:Proteoglycans / Q000032:analysis; D013526:Surgical Mesh; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D004343:Drug Implants; D016222:Fibroblast Growth Factor 2; D037121:Plant Lectins; D010982:Platelet-Derived Growth Factor; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C045475:Griffonia simplicifolia lectins