title: RPA regulates telomerase action by providing Est1p access to chromosome ends.
abstract: Replication protein A (RPA) is a highly conserved single-stranded DNA-binding protein involved in DNA replication, recombination and repair. We show here that RPA is present at the telomeres of the budding yeast Saccharomyces cerevisiae, with a maximal association in S phase. A truncation of the N-terminal region of Rfa2p (associated with the rfa2Delta40 mutated allele) results in severe telomere shortening caused by a defect in the in vivo regulation of telomerase activity. Cells carrying rfa2Delta40 show impaired binding of the protein Est1p, which is required for telomerase action. In addition, normal telomere length can be restored by expressing a Cdc13-Est1p hybrid protein. These findings indicate that RPA activates telomerase by loading Est1p onto telomeres during S phase. We propose a model of in vivo telomerase action that involves synergistic action of RPA and Cdc13p at the G-rich 3' overhang of telomeric DNA.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Centre Nationale de la Recherche Scientifique, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Vera
authors.initials: V
authors.lastname: Schramke
authors.forename: Pierre
authors.initials: P
authors.lastname: Luciano
authors.forename: Vanessa
authors.initials: V
authors.lastname: Brevet
authors.forename: Sylvine
authors.initials: S
authors.lastname: Guillot
authors.forename: Yves
authors.initials: Y
authors.lastname: Corda
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Longhese
authors.forename: Eric
authors.initials: E
authors.lastname: Gilson
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
mesh_terms: D004261:DNA Replication; D004271:DNA, Fungal; D004268:DNA-Binding Proteins / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D051716:Replication Protein A; D016196:S Phase / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000737:chemistry; D034501:Telomere-Binding Proteins / Q000378:metabolism
chemical_list: C452219:Cdc13 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D051716:Replication Protein A; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; C099024:EST1 protein, S cerevisiae; D019098:Telomerase