[Sequence detection of HBV-DNA P and C region in HIV/HBV superinfection subjects with drug resistance to HAART].
To further study the resistance of HBV to high activity antiretrovirus therapy(HAART).



HBV-DNA was quantitatively detected by real-time PCR in 36 HIV/HBV superinfection subjects, C region and P region HBV-DNA in high copies HBV-DNA subjects were detected by routine PCR, PCR products were purified and sequenced and compared with the HBV international Genbank using BLSAT softy ware.



HBV-DNA was positive in 4 of 36 patients (11.1%) and another 3 had low copies(<10(4)copies/ml), one had a high HBV-DNA copies (10(7)copies/ml). It's HBV-DNA C region sequence had mutation on 2 sites (nt 2412 T/C; nt 2413 T/C) and 1 mutation P region (nt 741 A/G, also YMDD/YVDD) compared with HBV international Genbak reference sequence.



The HBV resistance to HAART may be related with multiple genetic mutations in the C and P regain of HBV-DNA.
ZHu
Biao
B
The Institute of Infectious Diseases, Zhejiang University, Hangzhou 310003, China.
Wu
Nan-ping
NP
Bader
Armin
A
Brockmeyer
Norbert
N
D023241:Antiretroviral Therapy, Highly Active; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry*; D024882:Drug Resistance, Viral; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D006509:Hepatitis B / Q000188:drug therapy* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation
D004279:DNA, Viral