title: [Genotype of human calicivirus from infants with diarrhea in Guangzhou city in 2001].
abstract: OBJECTIVE
  To understand the characteristics of human calicivirus (HuCV) infection in infants with diarrhea in Guangzhou city and to study genotype of the virus.
  
  
  METHODS
  The authors collected fecal specimens from 22 children with acute nonbacterial gastroenteritis from November to December, 2001. HuCV was detected from the specimens by RT-PCR. The PCR products were cloned into the PMD18-T cloning vector and sequenced.
  
  
  RESULTS
  HCV was detected from the specimens of 2 cases (9%, 2/22). The nucleotide sequence analysis revealed that the virus strains belonged to genotype 2 of Norwalk-like viruses.
  
  
  CONCLUSIONS
  HuCV is one of the pathogens causing diarrhea in infants and young children in Guangzhou area. HuCV infection occurred sporadically in autumn and winter.
authors.affiliation: Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xie
authors.forename: Zhao-yin
authors.initials: ZY
authors.lastname: Fang
authors.forename: Si-tang
authors.initials: ST
authors.lastname: Gong
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.forename: Wen-ji
authors.initials: WJ
authors.lastname: Ou
authors.forename: Gen-ping
authors.initials: GP
authors.lastname: Wan
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Lai
authors.forename: Cui-juan
authors.initials: CJ
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002139:Caliciviridae / Q000235:genetics*; D017250:Caliciviridae Infections / Q000150:complications* / Q000821:virology; D002681:China; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003968:Diarrhea, Infantile / Q000209:etiology*; D004403:Dysentery / Q000209:etiology*; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral