Microemulsion electrokinetic chromatography of drugs varying in charge and hydrophobicity: I. Impact of parameters on separation performance evaluated by multiple linear regression models.
The separation of anionic, cationic and neutral drugs in microemulsion electrokinetic chromatography (MEEKC) was studied with a statistical experimental design. The concentration of sodium dodecyl sulfate (SDS, surfactant), 1-butanol (co-surfactant) and borate buffer and the factors Brij 35 (surfactant), 2-propanol (organic solvent) and cassette temperature were varied simultaneously, while the parameters pH (9.2), the concentration of octane (oil, 0.8% w/w), the voltage (10 kV) and the dimension of the fused-silica capillary, were kept constant. Eight different model substances were chosen with different hydrophobicities. Two of the analytes were positively charged, two were negatively charged, and the remaining four were neutral or close to neutral at the pH explored. The importance of each parameter on the separation window, the plate height and the retention factor for each of the analytes was studied by means of multiple linear regression (MLR) models. A new response was evaluated for anions, the quotient between the effective mobility in the microemulsion and the effective mobility in the corresponding buffer. Factors affecting selectivity changes were also explored, and it was found that SDS and 2-propanol had the largest effect on selectivity.
Harang
Valérie
V
Analytical Development, Pharmaceutical and Analytical R&D, AstraZeneca, Södertälje, Sweden.
Eriksson
Jessica
J
Sänger-van de Griend
Cari E
CE
Jacobsson
Sven P
SP
Westerlund
Douglas
D
D019840:2-Propanol; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification*; D012044:Regression Analysis; D012967:Sodium Dodecyl Sulfate; D055672:Static Electricity; D013696:Temperature
D002021:Buffers; D004364:Pharmaceutical Preparations; D012967:Sodium Dodecyl Sulfate; D019840:2-Propanol