title: Purification of highly-active and soluble Escherichia coli sigma 70 polypeptide overproduced at low temperature.
authors.affiliation: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892, USA.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhi
authors.forename: Ding Jun
authors.initials: DJ
authors.lastname: Jin
mesh_terms: D001671:Biochemistry / Q000379:methods*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D012808:Sigma Factor / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases