NF-kappaB activation at implantation window of the mouse uterus.
OBJECTIVE
Nuclear factor kappa B (NF-kappaB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-kappaB at implantation window is unknown.


METHODS
Activation of NF-kappaB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-kappaB, was analyzed by immunohistochemistry.


RESULTS
NF-kappaB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-kappaB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells.


CONCLUSIONS
NF-kappaB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.
Nakamura
Hitomi
H
Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan.
Kimura
Tadashi
T
Ogita
Kazuhide
K
Nakamura
Takafumi
T
Takemura
Masahiko
M
Shimoya
Koichiro
K
Koyama
Sinsuke
S
Tsujie
Tomoko
T
Koyama
Masayasu
M
Murata
Yuji
Y
D000818:Animals; D024202:Electrophoretic Mobility Shift Assay; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism*; D011247:Pregnancy; D014599:Uterus / Q000378:metabolism* / Q000502:physiology
D016328:NF-kappa B