[Statistical fluctuations in processes of gene expression regulation: consideration of the problem from point of view of statistical mechanics].
The regulation of gene expression is a basic problem of biology. In some cases, the gene activity is regulated by specific binding of regulatory proteins to DNA. In terms of statistical mechanics, this binding is described as the process of adsorption of ligands on the one-dimensional lattice and has a probability nature. As a random physical process, the adsorption of regulatory proteins on DNA introduces a noise to the regulation of gene activity. We derived equations, which make it possible to estimate this noise in the case of the binding of the lac repressor to the operator and showed that these estimates correspond to experimental data. Many ligands are able to bind nonspecifically to DNA. Nonspecific binding is characterized by a lesser equilibrium constant but a greater number of binding sites on the DNA, as compared with specific binding. Relations are presented, which enable one to estimate the probability of the binding of a ligand on a specific site and on nonspecific sites on DNA. The competition between specific and nonspecific binding of regulatory proteins plays a great role in the regulation of gene activity. Similar to the one-dimensional "lattice gas" of particles, ligands adsorbed on DNA produce "one-dimensional" pressure on proteins located at the termini of free regions of DNA. This pressure, an analog of osmotic pressure, may be of importance in processes leading to changes in chromatin structure and activation of gene expression.
Nechipurenko
Iu D
IuD
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Physical Department, Lomonosov Moscow State University, Bioanaliticheskie Tekhnologii Company, Moscow, Russia.
Wolf
A M
AM
Gurskiĭ
G V
GV
D000327:Adsorption; D004247:DNA / Q000378:metabolism*; D005786:Gene Expression Regulation; D008024:Ligands; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism
D008024:Ligands; D012097:Repressor Proteins; D004247:DNA