In vitro release of mitomycin C from collagen implants.
OBJECTIVE
To study Mitomycin C Loaded Collagen Implant (CI) pharmacokinetics behaviour in vitro.


METHODS
The CI were incubated for 15 minutes in different MMC loading solutions with the following concentrations: 0.03 mg/mL (n = 9), 0.3 mg/mL (n = 10) and 3.0 mg/mL (n = 10). The loaded CI were transferred in 100 micro L of 0.9% NaCl. Aqueous flow of 5 micro L/min was simulated. The MMC concentrations of the samples were determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined.


RESULTS
The CI absorbed on average a MMC dose of 0.054, 0.530 and 6.090 micro g when incubated in the different MMC loading solutions containing 0.03 mg/mL, 0.3 mg/mL, 3.0 mg/mL of MMC, respectively. In the release experiments, the mean total dose delivered by CI was 0.0493, 0.585 and 5.291 micro g. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast MMC dissolution. The half-life of the 3 series was 8.8, 10.1 and 10.5 min.


CONCLUSIONS
Commercially available CI can be loaded with MMC, and could provide relatively slower release than sponge delivery of MMC. Clinical implications of these results warrants further studies.
Zimmerman
C
C
Department of Clinical Pharmacology, University Hospital, Basel, Switzerland.
Drewe
J
J
Flammer
J
J
Shaarawy
T
T
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism*; D004337:Drug Carriers; D006207:Half-Life; D020007:Implants, Experimental; D016685:Mitomycin / Q000493:pharmacokinetics*; D012590:Sclera / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D013552:Swine
D004337:Drug Carriers; D012965:Sodium Chloride; D016685:Mitomycin; D003094:Collagen