Cluster analysis of genes with significant change in expression in cells conditioned to survive TBOOH.
Immortal murine lens epithelial cells, alphaTN4-1 have been conditioned to survive H2O2, H cells, or TBOOH, T cells, at concentrations that will cause cataract in vitro. Since H cells are killed by TBOOH but T cells survive H2O2, it is of interest to examine the gene expression of these cell lines. We now report the results of cluster analysis of genes whose expression is significantly changed by TBOOH. The analysis has revealed a small group of antioxidative defense genes that contribute to the survival of T and H cells when exposed to oxidative stress.
Li
Dayu
D
Ma
Wanchao
W
Sun
Fang
F
Pavlidis
Paul
P
Spector
Abraham
A
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016000:Cluster Analysis; D004847:Epithelial Cells / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007908:Lens, Crystalline / Q000187:drug effects*; D051379:Mice; D018384:Oxidative Stress; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
D020122:tert-Butylhydroperoxide