Involvement of CRF but not NPY in the anxiety regulation via NMDA receptors.
The study attempts to evaluate whether neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) are involved in anxiogenic and anxiolytic reactions induced by NMDA receptor ligands. The animals were given MK-801 (1 mg/kg, ip), a non-competitive NMDA-receptor antagonist, which acts as anxiolytic agent, or NMDA (15 mg/kg, ip), which has an anxiogenic effect. The anxiogenic or anxiolytic actions of these compounds were evaluated in the plus-maze test. The animals, which were given MK-801, were administered BIBO 3304 (130 ng/0.5 microl/site) intraamygdalarly and the animals which were given NMDA were administered alpha-helical CRF (500 ng/0.5 microl/site). BIBO 3304 did not attenuate MK-801-induced anxiolysis and alpha-helical CRF abolished NMDA-induced anxiogenesis. Our results show that anxiogenic effect of NMDA is mediated via CRF1 receptors and anxiolytic action of MK-801 is not dependent on Y1 receptors.
Wierońska
Joanna M
JM
Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, PL 31-343 Kraków, Poland.
Szewczyk
Bernadeta
B
Pałucha
Agnieszka
A
Brański
Piotr
P
Smiałowska
Maria
M
D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001007:Anxiety / Q000188:drug therapy / Q000378:metabolism*; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008024:Ligands; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D016202:N-Methylaspartate / Q000494:pharmacology; D009478:Neuropeptide Y / Q000378:metabolism*; D017433:Protein Structure, Secondary; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors
D014151:Anti-Anxiety Agents; D008024:Ligands; D009478:Neuropeptide Y; D018019:Receptors, Corticotropin-Releasing Hormone; D016194:Receptors, N-Methyl-D-Aspartate; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D003346:Corticotropin-Releasing Hormone; D001120:Arginine; C117416:BIBO 3304