A simple protocol for isolation of fungal DNA.
Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.
Tendulkar
Sachin R
SR
Department of Microbiology and Biotechnology Center, Faculty of Science, M.S. University of Baroda, Vadodara 390002 (Gujarat), India.
Gupta
Archna
A
Chattoo
Bharat B
BB
D004271:DNA, Fungal / Q000737:chemistry* / Q000302:isolation & purification*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D020082:Magnaporthe / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D008832:Microchemistry / Q000379:methods*; D008872:Microwaves; D011829:Radiation Dosage
D004271:DNA, Fungal