title: [Noninvasive continuous measurement of blood glucose concentration via animal skin].
abstract: In this study we deliberated over the principles and methods and then took the noninvasive continuous measurement of blood glucose concentration through the skin of rabbits. The glucose oxidase sensor was made by covalent immobilization. The best making method of sensor and stable working condition were sifted. Ten female and 10 male adult white rabbits were allocated into the groups of the ante-ultrasound and post-ultrasound, the injection of glucose, and the high and low frequency ultrasounds. After the skin surface was treated by high or low frenquency ultrasound for 5 minutes on the rabbits, obvious changes (P < 0.01) of post-ultrasound and post-injection of glucose were observed by means of glucose oxidase sensor and microcurrent apparatus. After application of ultrasound to the skin of rabbits, the penetration of glucose through the rabbit skin increased obviously. The change of microcurrent signal that was exchanged by the glucose sensor correlated positively with the concentration of glucose of rabbit body. The blood glucose can be tested by the glucose sensor on the skin surface of living animal.
authors.affiliation: Sichuan Cancer Hosiptal, Chengdu 610041.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gao
authors.forename: Suping
authors.initials: S
authors.lastname: Wei
authors.forename: Shibi
authors.initials: S
authors.lastname: Zhang
authors.forename: Junguo
authors.initials: J
authors.lastname: Ran
authors.forename: Li
authors.initials: L
authors.lastname: Gou
authors.forename: Song
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D005260:Female; D005949:Glucose Oxidase; D008297:Male; D011817:Rabbits; D012867:Skin / Q000528:radiation effects; D013010:Sonication
chemical_list: D001786:Blood Glucose; D005949:Glucose Oxidase