A group of related cDNAs encoding secreted proteins from Hessian fly [Mayetiola destructor (Say)] salivary glands.
A group of cDNAs has been isolated and characterized from Hessian fly [Mayetiola destructor (Say)] salivary glands. Members in this group appear to encode proteins with secretion signal peptides at the N-terminals. The mature putative proteins are small, basic proteins with calculated molecular weights that ranged from 8.5 to 10 kDa, and isoelectric points from 9.92 to 10.90. Sequence analysis indicated a strong selection for mutations that generate amino acid changes within the coding region. Northern blot analysis revealed that these genes are expressed only in the first instar larvae, a critical stage that determines if the interaction between a specific Hessian fly biotype and a specific wheat cultivar is compatible. Genomic analysis demonstrated that multiple copies of similar genes are clustered within a short region on chromosome 2A. This is the same arm in which two avirulence genes have been mapped.
Chen
M-S
MS
USDA-ARS, Plant Science and Entomology Research Unit, Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. mchen@ksu.edu
Fellers
J P
JP
Stuart
J J
JJ
Reese
J C
JC
Liu
X
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial; D004175:Diptera / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics; D012463:Saliva / Q000737:chemistry*; D012469:Salivary Glands / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology