Myoblast migration is prevented by a calpain-dependent accumulation of MARCKS.
Calpains, also called calcium activated neutral cysteine proteases are presently known to play pivotal roles in physiological and biological phenomena such as signal transduction, cell spreading and motility, apoptosis, regulation of cell cycle and regulation of muscle cell differentiation. Concerning this last point, calpains have been shown to play a crucial role during the earlier myogenesis. In this study we have analyzed the involvement of calpains during an important step of myogenesis: myoblast migration. Our findings show that myoblast migration was drastically reduced when the expression of micro- and m-calpain was decreased. We have also observed that MARCKS (myristoylated alanine rich C kinase substrate), a protein localized at focal adhesion sites, was significantly accumulated when the expression levels of calpains were decreased. Also, using phorbol myristate acetate, (an activator of PKC) and plasmids carrying the full-length cDNA of MARCKS or a cDNA fragment lacking the phosphorylation site domain, we demonstrated that normal myoblast migration is dependent on MARCKS phosphorylation and localization.
Dedieu
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Laboratoire Biosciences de l'Aliment, ISTAB USC-INRA 429, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France.
Mazères
G
G
Poussard
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Brustis
J-J
JJ
Cottin
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P
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001665:Binding Sites / Q000235:genetics; D002154:Calpain / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D015151:Immunoblotting; D047908:Intracellular Signaling Peptides and Proteins; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D010766:Phosphorylation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D047908:Intracellular Signaling Peptides and Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D002154:Calpain; D013755:Tetradecanoylphorbol Acetate