title: [Isolation and purification of bovine testicular hyaluronidase].
abstract: We developed a single-step method for chromatographic purification of the hyaluronidase extract on Sepharose blue, which produced high yield of the enzyme (95%) with high purity (40). Purification of hyaluronidase allowed us to obtain biological preparations standardized by the protein composition and enzyme activity and useful for basic and applied researches.
authors.affiliation: Udmurt State University, Izhevsk, 426034 Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Barsukov
authors.forename: O V
authors.initials: OV
authors.lastname: Kozhevnikova
authors.forename: A V
authors.initials: AV
authors.lastname: Khokhriakova
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification*; D008297:Male; D011786:Quality Control; D012685:Sepharose; D013737:Testis / Q000201:enzymology*
chemical_list: D012685:Sepharose; D006821:Hyaluronoglucosaminidase