title: Putrescine, cadaverine, and indole production by bacteria isolated from wild and aquacultured penaeid shrimp stored at 0, 12, 24, and 36 degrees C.
abstract: Putrescine, cadaverine, and indole production capabilities of bacteria isolated from wild domestic and aquacultured Ni-caraguan penaeid shrimp in progressive decomposition states were evaluated. The numbers and types of microorganisms responsible for the production of putrescine, cadaverine, and indole in wild and aquacultured shrimp increased with increasing decomposition temperature and time. Throughout the storage experiments, mean aerobic plate counts (log/g) ranged from 4.5 to 9.7 and 4.5 to 9.0 for domestic and Nicaraguan shrimp, respectively. Vibrio spp. were more prominent in Nicaraguan shrimp (Litopenaeus vannamei) than in domestic shrimp (Litopenaeus setiferus and Litopenaeus brasiliensis). The only amine-producing (putrescine) microorganism isolated from wild and aquacultured shrimp at all temperatures of decomposition (0, 12, 24, and 36 degrees C) was Shewanella putrefaciens. On the basis of putrescine production by S. putrefaciens at 0 and 12 degrees C and putrescine production by S. putrefaciens, Vibrio spp., and Morganella morganii at 24 and 36 degrees C, putrescine should be considered a potential chemical indicator of decomposition in shrimp.
authors.affiliation: Aquatic Food Products Laboratory, Food Science and Human Nutrition Department, PO Box 110370, University of Florida, Gainesville, Florida 32611-0370, USA. rbenner@cfsan.fda.gov
authors.forename: R A
authors.initials: RA
authors.lastname: Benner
authors.forename: W F
authors.initials: WF
authors.lastname: Staruszkiewicz
authors.forename: W S
authors.initials: WS
authors.lastname: Otwell
mesh_terms: D000818:Animals; D000835:Animals, Wild; D017756:Aquaculture; D002103:Cadaverine / Q000032:analysis / Q000096:biosynthesis*; D015169:Colony Count, Microbial; D007211:Indoles / Q000032:analysis; D020613:Morganella morganii / Q000302:isolation & purification / Q000378:metabolism; D033561:Penaeidae / Q000382:microbiology*; D011700:Putrescine / Q000032:analysis / Q000096:biosynthesis*; D020592:Shewanella / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014733:Vibrio / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D007211:Indoles; C030374:indole; D002103:Cadaverine; D011700:Putrescine