title: Aminopeptidases in visceral organs during alterations in body fluid volume and osmolality.
abstract: Enzymatic cleavage of some peptides in the local environment could be included among the mechanisms related to the regulation of hydrosaline balance. In order to examine this hypothesis, we measured representative aminopeptidase activities in visceral organs of rats after applying certain hydrosaline challenges. Decreased levels (about 30%) of particulate puromycin-insensitive-neutral aminopeptidase in the renal medulla and of soluble acid aminopeptidase in the lung were observed under hyperosmolality and hypovolemia. Decreased levels (more than 45%) of particulate type-I-pyroglutamyl aminopeptidase in the heart were observed under altered volemia. These results indicate that aminopeptidases at these anatomical locations might be involved in the regulation of body fluid volume and osmolality.
authors.affiliation: Laboratory of Pharmacology, Instituto Butantan, Av. Vital Brazil 1500, 05503-900, São Paulo, SP, Brazil.
authors.forename: Elaine
authors.initials: E
authors.lastname: Gasparello-Clemente
authors.forename: Luis
authors.initials: L
authors.lastname: Casis
authors.forename: Adolfo
authors.initials: A
authors.lastname: Varona
authors.forename: Javier
authors.initials: J
authors.lastname: Gil
authors.forename: Jon
authors.initials: J
authors.lastname: Irazusta
authors.forename: Paulo Flávio
authors.initials: PF
authors.lastname: Silveira
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001769:Blood / Q000378:metabolism; D001826:Body Fluids / Q000502:physiology*; D001835:Body Weight; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D014781:Viscera / Q000201:enzymology*; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D000626:Aminopeptidases