Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.
The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.
Aizawa
Hiroyuki
H
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Hu
Shu-Ching
SC
Bobb
Kathryn
K
Balakrishnan
Karthik
K
Ince
Gulayse
G
Gurevich
Inga
I
Cowan
Mitra
M
Ghosh
Anirvan
A
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D050882:CREB-Binding Protein; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D003001:Cloning, Molecular; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D018390:Gene Targeting; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009154:Mutation; D009420:Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
D015220:Calcium Channels; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C481041:Ss18l1 protein, rat; D015534:Trans-Activators; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C494364:Crebbp protein, mouse; C494365:Crebbp protein, rat; D002118:Calcium