Molecular cloning and expression of bottlenose dolphin (Tursiops truncatus) interleukin-8.
The bottlenose dolphin interleukin (IL)-8 cDNA was molecularly cloned. The dolphin IL-8 has an open reading frame of 303-bp encoding 101 amino acids. The homology of the amino acid sequence with that of other species was: sheep, 89.1%; cattle, 88.1%; pig, 85.1%; dog, 85.1%; horse, 79.2%; human, 74.5%; and macaque, 72.3%. The amino acid sequence suggested that dolphin IL-8 was a CXC chemokine. The recombinant dolphin IL-8 protein was recognized with anti-ovine IL-8 monoclonal antibody.
Itou
Takuya
T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Yoshida
Yuuki
Y
Shoji
Yoko
Y
Sugisawa
Hitoshi
H
Endo
Tomoko
T
Sakai
Takeo
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004289:Dolphins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D016209:Interleukin-8