Detection of the putative cis-region involved in the induction by a Pyricularia oryzae elicitor of the promoter of a gene encoding phenylalanine ammonia-lyase in rice.
A rice PAL (phenylalanine ammonia-lyase) gene sequence ( rPAL-P5), which is highly similar to and likely the same as a previously described rice ZB8PAL gene, including the 5'-upstream and exon I coding regions of PAL, was isolated using PCR amplification. The expression of several PALs, including rPAL-P5, was strongly induced following inoculation with Pyricularia oryzae or treatment with a P. oryzae elicitor. To identify the promoter region induced by the P. oryzae elicitor, we constructed and subsequently transformed rPAL-P5 promoter deletion series into rice calli using particle bombardment. Results from both elicitor-inducible reporter gene and gel mobility shift assays demonstrated that the sequence -349 to -256 of the rPAL-P5 promoter includes a cis-element involved in the induction of P. oryzae.
Wang
L
L
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871 Beijing, PR China.
An
C
C
Qian
W
W
Liu
T
T
Li
J
J
Chen
Z
Z
D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020082:Magnaporthe / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid
D018744:DNA, Plant; D010650:Phenylalanine Ammonia-Lyase