title: Fourier transform infrared microscopic imaging: effects of estrogen and estrogen deficiency on fracture healing in rat femurs.
abstract: Infrared spectroscopic imaging with 6-10 microm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.
authors.affiliation: Rutgers University, Department of Chemistry, Newark, New Jersey 07102, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Ouyang
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Sherman
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Paschalis
authors.forename: Adele L
authors.initials: AL
authors.lastname: Boskey
authors.forename: Richard
authors.initials: R
authors.lastname: Mendelsohn
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D004967:Estrogens / Q000008:administration & dosage* / Q000172:deficiency*; D005260:Female; D005264:Femoral Fractures / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology*; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D004967:Estrogens