title: [Expression and significance of substance P, neurofilament-H in glomus tumors with chronic pain].
abstract: OBJECTIVE
  To observe the expression and distribution of substance P (SP), neurofilament-H (NFH) in glomus tumors with chronic pain, and to discuss the process of chronic pain and the relationship with pain degree.
  
  
  METHODS
  Twenty-seven patients diagnosed as glomus tumor with chronic pain were enrolled as case group, and divided into light pain symptomatic group (LPSG) (n = 12) and severe pain symptomatic group (SPSG) (n = 15) according to clinical manifestations. Control group (CG) were enrolled by 30 patients with amputated extremities or hands after trauma. Immunohistochemical methods were used to determine the expression of SP, NFH which were detected quantitatively by computer graph analysis system too.
  
  
  RESULTS
  The positive expression and distribution of SP, NFH existed in all the three groups and SPSG expression level was the highest [Grayscale Value(SP) (143.3 +/- 7.5), Grayscale Value(NFH) (167.7 +/- 4.4)], LPSG followed [Grayscale Value(SP) (156.2 +/- 8.2), Grayscale Value(NFH) (194.8 +/- 4.0)], control group was the third [Grayscale Value(SP) (208.2 +/- 16.6), Grayscale Value(NFH) (225.1 +/- 8.3)]; The difference of expression level among three groups was significant [SPSG vs LPSG (P(SP) = 0.002, P(NFH) < 0.0001), SPSG vs CG (P(SP) < 0.0001, P(NFH) < 0.0001), LPSG vs CG (P(SP) < 0.0001, P(NFH) < 0.0001)]. The findings of Pearson product-moment correlation analysis between quantitative grayscale value of SP, NFH respectively and pain score in all the patients with glomus tumor showed linear negative correlation (r(SP) = -0.8974, P(SP) = 0.000001; r(NFH) = -0.6545, P(NFH) = 0.000212).
  
  
  CONCLUSIONS
  SP is the mainly afferent pain transmitter in the process of chronic pain in glomus tumor, and NFH plays an important role in pain-transmitted activities.
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
authors.forename: Jun-song
authors.initials: JS
authors.lastname: Wu
authors.forename: Zong-jian
authors.initials: ZJ
authors.lastname: Huang
authors.forename: Jun-fu
authors.initials: JF
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Ma-rong
authors.initials: MR
authors.lastname: Fang
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Pang
authors.forename: He-de
authors.initials: HD
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D002908:Chronic Disease; D005260:Female; D005918:Glomus Tumor / Q000737:chemistry / Q000503:physiopathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000032:analysis*; D010148:Intractable Pain / Q000209:etiology*; D013373:Substance P / Q000032:analysis*
chemical_list: D016900:Neurofilament Proteins; C051774:neurofilament protein H; D013373:Substance P