title: [Human placenta derived adherent cells support in vitro expansion of umbilical cord blood CD34+ cells].
abstract: The objective of this study was to elucidate the effect of human placenta adherent cells (hPDAC) on expansion of human umbilical cord blood CD34(+) cells in vitro. hPDAC was isolated and characterized in human placenta tissue by using enzyme-digesting method and flow cytometry. A co-culture system was established with hPDAC and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of hPDAC and SCF, IL-3, IL-6 and FL. The number of total nucleated cells, CFC and CD34(+) cells were repeatedly counted in culture for 4 weeks. The results showed that the hPDAC displayed fibroblast-like morphology, and were positive for CD29, CD44, CD166, HLA-ABC and UEA-1, and negative for CD34, CD45 and HLA-DR. Functionally, ex vivo expansion of CD34(+) cells on feeder layer of placental adherent cells was significantly higher than that no feeder layer group. SCF + IL-3 + IL-6 + FL + hPDAC manifested the most potent combination, with the number of total nucleated cells increasing by (126.0 +/- 6.7)-fold, progenitor cells (CFC) (49.8 +/- 1.7)-fold and CD34(+) cells (8.3 +/- 1.65)-fold. It is concluded that placental adherent cells could support hematopoiesis in vitro and work as a suitable feeder layer for cord blood stem/progenitor cell expansion.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. zhangyi@nic.bmi.ac.cn
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.forename: Pei-Hsien
authors.initials: PH
authors.lastname: Tang
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D002448:Cell Adhesion; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D010920:Placenta / Q000166:cytology*
chemical_list: D018952:Antigens, CD34