1-Methyl-3-nitropyridine: an efficient oxidant of NADH in non-enzymatic and enzyme-mediated processes.
It is shown that NADH can be effectively oxidized by 1-methyl-3-nitropyridine in non-enzymatic and enzyme-mediated processes. Mechanistic issues of these reactions are discussed. These processes seem to contribute to the observed cytotoxicity of 1-methyl-3-nitropyridine. A key role of 1-methyl-3-nitropyridinyl radical formed in the enzyme-mediated processes is emphasized.
Wieczorkowska
Marzena
M
Institute of Applied Radiation Chemistry, Technical University, Zeromskiego 116, 90-924 Lodz, Poland.
Zielonka
Jacek
J
Celińska
Joanna
J
Adamus
Jan
J
Ciesielska
Ewa
E
Marcinek
Andrzej
A
Szmigiero
Leszek
L
Gebicki
Jerzy
J
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005609:Free Radicals / Q000378:metabolism; D007700:Kinetics; D007939:Leukemia L1210; D009243:NAD / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011726:Pyridinium Compounds / Q000378:metabolism / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
C488480:1-methyl-3-nitropyridinium; D005609:Free Radicals; D016877:Oxidants; D011726:Pyridinium Compounds; D009243:NAD; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D009245:NADH Dehydrogenase; D010100:Oxygen