Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.
Nucleotide sequence differences in the acetylcholinesterase gene Ace.2 were used to develop an assay to distinguish among North American mosquitoes of the Culex pipiens complex. Taxon-specific polymerase chain reaction primers based on sequence differences within intron 2 of Ace.2 distinguish among the sibling species Cx. pipiens Linneaus and Cx. quinquefasciatus Say and their F1 hybrids. This assay may be used to confirm the species composition of mosquito pools, identify individual specimens collected in arbovirus surveillance programs and other mosquito studies, and define zones of hybridization.
Aspen
Stephen
S
Division of Vector-Borne Infectious Disease, Centers for Disease Control and Prevention, PO Box 2087, Fort Collins, CO 80522, USA.
Savage
Harry M
HM
D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D003465:Culex / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D009656:North America; D016133:Polymerase Chain Reaction
D000110:Acetylcholinesterase