Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia.
Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia.
Zhu
Liang
L
Departments of Civil Engineering, National University of Singapore, Singapore.
Ang
Simon
S
Liu
Wen-Tso
WT
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D016829:Giardia lamblia / Q000302:isolation & purification*; D045663:Quantum Dots
D000913:Antibodies, Protozoan; D005456:Fluorescent Dyes