title: Accuracy of pulse oximetry readings in an animal model of low perfusion caused by emerging pneumonia and sepsis.
abstract: OBJECTIVE
  To test the effects of low perfusion caused by emerging sepsis on the performance of two new pulse oximetry techniques: Masimo SET in comparison with Nellcor Oxismart XL.
  
  
  METHODS
  Cohort study with random allocation of two pulse oximetry devices to two sensor sites. SETTING. University animal research facility.
  
  
  METHODS
  Twenty-five adult, anesthetized, ventilated rabbits.
  
  
  METHODS
  Pneumonia/sepsis was induced by tracheal instillation of E. coli.
  
  
  RESULTS
  Oxygen saturation was measured by pulse oximetry (SpO(2)) and recorded continuously until death. Arterial oxygen saturation (SaO(2)) was measured hourly by CO oximetry and whenever a difference of >5% between the devices occurred. SpO(2) sensors were positioned at both forelegs and switched hourly. There was no difference in total signal dropout time [median 3.8 min (range 0.4-66.6 min) vs 3.3 min (range 0-94.5 min), Masimo SET vs Oxismart XL]. There were fewer episodes with a false SpO(2) reading [1 (range 0-7) vs 2 (range 0-17)] using the Masimo SET vs the Oxismart XL as verified by CO oximetry; p<0.05. Average bias (SpO(2)-SaO(2)) was significantly different between the two devices, and variability of bias values increased across time with both devices.
  
  
  CONCLUSIONS
  Both devices were capable to measure SpO(2) during most of the experimental time in this model of low perfusion and therefore appear to be highly sensitive to pick up a signal; however, low perfusion caused by emerging sepsis may result in inaccurate measurements with both devices. These episodes were less common with the Masimo SET vs the Oxismart XL.
authors.affiliation: Division of Neonatology and Pediatric Critical Care, Department of Pediatrics, Children's Hospital, University of Ulm, 89070 Ulm, Germany. helmut.hummler@medizin.uni-ulm.de
authors.forename: Helmut D
authors.initials: HD
authors.lastname: Hummler
authors.forename: Anja
authors.initials: A
authors.lastname: Engelmann
authors.forename: Frank
authors.initials: F
authors.lastname: Pohlandt
authors.forename: Josef
authors.initials: J
authors.lastname: Högel
authors.forename: Axel R
authors.initials: AR
authors.lastname: Franz
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D015331:Cohort Studies; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000150:complications; D023421:Models, Animal; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D018410:Pneumonia, Bacterial / Q000209:etiology / Q000503:physiopathology*; D011817:Rabbits; D011897:Random Allocation; D012129:Respiratory Function Tests; D018805:Sepsis / Q000209:etiology / Q000503:physiopathology*
chemical_list: D010100:Oxygen