title: Potent cross-group neutralization of primary human immunodeficiency virus isolates with monoclonal antibodies--implications for acquired immunodeficiency syndrome vaccine.
abstract: Human immunodeficiency virus type 1 (HIV-1) is phylogenetically classified into groups and clades (or subtypes). Human neutralizing monoclonal antibodies (nMAbs), originally isolated from individuals infected with HIV-1 group M-clade B, neutralized not only primary HIV-1 clade B isolates in vitro but also primary isolates of other group M clades (A, C, D, E, and F). This corrected the previously held notion that primary HIV-1 isolates are resistant to neutralizing antibodies. Here we show that anti-HIV-1 group M-clade B nMAbs potently neutralized primary isolates of the phylogenetically distant HIV-1 group O. We and others have previously shown that passive immunization with human nMAbs protected adult or neonatal primates against infection with simian-human immunodeficiency virus strains encoding HIV-1 group M-clade B envelope genes. The in vitro cross-group neutralization shown here underscores the broad potential of these nMAbs against divergent virus variants and the relevance of their epitopes in the design of acquired immunodeficiency syndrome vaccines.
authors.affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Flavia
authors.initials: F
authors.lastname: Ferrantelli
authors.forename: Moiz
authors.initials: M
authors.lastname: Kitabwalla
authors.forename: Robert A
authors.initials: RA
authors.lastname: Rasmussen
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Cao
authors.forename: Ting-Chao
authors.initials: TC
authors.lastname: Chou
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Gabriela
authors.initials: G
authors.lastname: Stiegler
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cavacini
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Joseph
authors.initials: J
authors.lastname: Cotropia
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Ugen
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Ruprecht
mesh_terms: D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D015195:Drug Design; D014644:Genetic Variation; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D009500:Neutralization Tests
chemical_list: D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D015483:HIV Antibodies