Isolation and characterization of the canine serotonin receptor 1B gene (htr1B).
The serotonin receptor 1B gene (htr1B) has been suggested to be implicated in mental disorders in both humans and other species. We have isolated a canine bacterial artificial chromosome (BAC) clone containing htr1B, revealed the coding and surrounding DNA sequence of canine htr1B and designed primer sets for genomic sequencing of the gene. A mutation scan in 10 dogs revealed five single nucleotide polymorphisms in the htr1B coding sequence. By random sequencing of subclones of the BAC a polymorphic microsatellite repeat was found. We found evidence for at least four extended haplotypes in six dogs of the same breed. The chromosomal localization of the gene was confirmed by fluorescence in situ hybridisation and radiation hybrid mapping. This work provides a starting point for mutation scans and association studies on dogs with behavioural problems.
van den Berg
Linda
L
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, 3584 CM, Utrecht, The Netherlands. L.vandenBerg@vet.uu.nl
Imholz
Sandra
S
Versteeg
Serge A
SA
Leegwater
Peter A J
PA
Zijlstra
Carla
C
Bosma
Anneke A
AA
van Oost
Bernard A
BA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs / Q000235:genetics*; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping; D044302:Receptor, Serotonin, 5-HT1B / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C470302:HTR1B protein, human; D044302:Receptor, Serotonin, 5-HT1B; D004247:DNA