title: [Preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin].
abstract: OBJECTIVE
  To study the preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin(BSA-NP-GL) targeting to hepatocytes.
  
  
  METHODS
  The bovine serum albumin nanoparticles (BSA-NP) were prepared by desolvation process. Glycyrrhizin (GL) was oxidized by sodium periodate to be conjugated to surface reactive amino groups (SRAG) of the BSA-NP. The SRAG were quantified by spectrophotometric method using 2, 4, 6-trinitrobenzenesulfonic acid(TNBS). Glycyrrhetinic acid(GA) hydrolyzed from GL, which was on the surface of BSA-NP-GL was assayed by HPLC after isolation by sephadex G-50. Both methods were used to verify the conjugation achieved. HPLC was used to determine surface density of GL on BSA-NP-GL.
  
  
  RESULTS
  The amount of SRAG of the BSA-NP-GL decreased by 19.6% compared with normal BSA-NP. The amount of GL molecule was 9.2% of the total determined SRAG of BSA-NP. The mean diameter of the BSA-NP-GL was 73 nm with round shape. The stability of BSA-NP-GL was constant when it was stored at 25 degrees C and 37 degrees C during 10 days.
  
  
  CONCLUSIONS
  BSA-NP-GL was successfully prepared, which is considered to establish an experimental foundation for further research on its ability for targeting to hepatocytes.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Sheng-jun
authors.initials: SJ
authors.lastname: Mao
authors.forename: Shi-xiang
authors.initials: SX
authors.lastname: Hou
authors.forename: Liang-ke
authors.initials: LK
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.forename: Yue-qi
authors.initials: YQ
authors.lastname: Bi
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D019695:Glycyrrhizic Acid / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D003432:Cross-Linking Reagents; D012710:Serum Albumin, Bovine; D019695:Glycyrrhizic Acid