Utilizing three-dimensional fluorescence's red-shift cascade effect to monitor mycobacterium PRY-1 degradation of aged petroleum.
Samples of Mycobacterium PRY-1 inoculated motor oil are subjected to three-dimensional fluorescence spectroscopy to document the shifting of excitation/emission maxima as the solutions undergo serial dilutions. Effects such as self-quenching of individual polycyclic aromatic hydrocarbons (PAHs) and energy transfer between PAHs combine to produce large red-shifts in the resulting fluorescence emission spectra. This process is repeated over a series of weeks and is compared to preceding spectra to gauge the microbial degradation of the petroleum. Results show a two-fold, or 75%, PAH contaminant degradation by Mycobacterium PRY-1 over a 140-day growth period.
Sinski
Joseph F
JF
Bellarmine University, Department of Chemistry, 2001 Newberg Road, Louisville, Kentucky 40205, USA. jsinski@bellarmine.edu
Compton
Bradley S
BS
Perkins
B Shea
BS
Nicoson
Michael C
MC
D001673:Biodegradation, Environmental; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D009161:Mycobacterium / Q000378:metabolism*; D010578:Petroleum / Q000032:analysis* / Q000382:microbiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004785:Environmental Pollutants; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons