Studies on the involvement of bradykinin using enalapril and 2-mercaptoethanol in ischemia-reperfusion induced myocardial infarction in albino rats.
The effects of bradykinin were evaluated using the ACE inhibitor enalapril and the APP inhibitor, 2-mercaptoethanol alone and in combination in rats with experimental myocardial infarction. Myocardial infarction was produced by occlusion of the left anterior descending coronary artery for 30 min followed by 4 h of reperfusion. Infarct size was measured by the TTC stain method. Lipid peroxide levels in serum and heart tissue were estimated by the methods developed by Yagi and Ohkawa et al., respectively. A lead II electrocardiogram was monitored throughout the experiment. With the combined inhibition of both the enzymes ACE and APP, a better cardioprotection was observed when compared to individual inhibition of the enzymes, suggesting the involvement of bradykinin during experimental myocardial infarction.
Akula
A
A
Pharmacology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India. annapurnaa@rediffmail.com
Veeravalli
K K
KK
Kota
M K
MK
Routhu
K V
KV
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000627:therapeutic use*; D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use*; D001920:Bradykinin / Q000502:physiology*; D003331:Coronary Vessels / Q000502:physiology; D004562:Electrocardiography / Q000187:drug effects; D004656:Enalapril / Q000627:therapeutic use*; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008623:Mercaptoethanol / Q000627:therapeutic use*; D009203:Myocardial Infarction / Q000473:pathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D008054:Lipid Peroxides; D008623:Mercaptoethanol; D004656:Enalapril; D001920:Bradykinin