title: Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: a high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract.
abstract: A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of approximately 50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Sunnie
authors.initials: S
authors.lastname: Myung
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Myeong Hee
authors.initials: MH
authors.lastname: Moon
authors.forename: John
authors.initials: J
authors.lastname: Taraszka
authors.forename: Rena
authors.initials: R
authors.lastname: Sowell
authors.forename: Stormy
authors.initials: S
authors.lastname: Koeniger
authors.forename: Amy E
authors.initials: AE
authors.lastname: Hilderbrand
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Valentine
authors.forename: Lucy
authors.initials: L
authors.lastname: Cherbas
authors.forename: Peter
authors.initials: P
authors.lastname: Cherbas
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Kaufmann
authors.forename: David F
authors.initials: DF
authors.lastname: Miller
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ewing
authors.forename: C Ray
authors.initials: CR
authors.lastname: Sporleder
authors.forename: David E
authors.initials: DE
authors.lastname: Clemmer
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D029721:Drosophila Proteins; D010455:Peptides