title: Different time course of changes in sarcoplasmic reticulum and myosin isoforms in rat soleus muscle at early stage of hyperthyroidism.
abstract: OBJECTIVE
  We studied changes in the expression of myosin and sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and in Ca2+ uptake and release rates by the SR in rat slow-twitch soleus muscles treated with thyroid hormone (3,5,3'-triiodo-l-thyronine, T3) for 1, 3, 7 and 21 days.
  
  
  RESULTS
  The content of fast SR Ca2+-ATPase isoform SERCA1a protein significantly increased as early as 3 days of T3 treatment, while a rise in fast myosin heavy chain isoforms was found only 21 days after the onset of T3 administration. T3-induced slow-to-fast transitions were much less pronounced in myosin light chain than in myosin heavy chain. Moreover, in vitro measurements of SR Ca2+-handling capacity with indo-1 indicated that increases in uptake rate preceded those of release rate (7 vs. 21 days).
  
  
  CONCLUSIONS
  These data suggest that changes in the myosin and SERCA expressions seem not to occur in a coordinated manner at the early stage of hyperthyroidism and that intervention with T3 may temporarily produce slow-twitch type I fibres which contain, in addition to slow SERCA2a isoform, the appreciable amounts of fast SERCA1a isoform. Moreover, it is speculated that some fibres in the hyperthyroid soleus muscles may temporarily acquire unique contractile properties distinct from normal fast and slow fibres.
authors.affiliation: Graduate School of Medical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Inashima
authors.forename: S
authors.initials: S
authors.lastname: Matsunaga
authors.forename: I
authors.initials: I
authors.lastname: Nara
authors.forename: H
authors.initials: H
authors.lastname: Kajihara
authors.forename: M
authors.initials: M
authors.lastname: Wada
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001835:Body Weight; D002118:Calcium; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D006980:Hyperthyroidism / Q000378:metabolism*; D007536:Isomerism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013997:Time Factors; D014284:Triiodothyronine / Q000008:administration & dosage
chemical_list: D018994:Myosin Light Chains; D014284:Triiodothyronine; D018995:Myosin Heavy Chains; D009218:Myosins; D000252:Calcium-Transporting ATPases; D002118:Calcium