Differential effect of lentil feeding on proteosynthesis rates in the large intestine, liver and muscle of rats.
The aim of this work was to test the hypothesis that the trophic effect of lentil feeding on large intestine results from a stimulation of protein synthesis and to determine whether it interferes with protein metabolism in other splanchnic or peripheral organs. Two groups of growing Sprague Dawley male rats were pair-fed iso-caloric iso-nitrogenous balanced diets containing either cooked lentils (Lens esculenta puyensis) or casein as unique protein source. Protein synthesis rates were measured in vivo, in large intestine, liver and gastrocnemius at the postprandial state. In large intestine, protein and ribonucleic acid contents were higher in the lentil-fed group than in the control group, and the amount of proteins synthesized was also higher (+57%). By contrast, liver protein and ribonucleic acid contents as well as protein synthesis rates were significantly lower in the lentil-fed group than in the control group. In the gastrocnemius muscle protein and ribonucleic acid contents were significantly lower and the amount of protein synthesized was also lower (-18%) in the lentil fed group than in the control group. This study suggests that stimulation of protein synthesis in the large intestine is compensated for by a decrease in liver and muscle.
Combe
Etiennette
E
Human Nutrition Research Centre and Institut National de la Recherche Agronomique, Clermont-Ferrand, France. combe@clermont.inra.fr
Pirman
Tatjana
T
Stekar
Jasna
J
Houlier
Marie-Louise
ML
Mirand
Philippe Patureau
PP
D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D004032:Diet; D004044:Dietary Proteins; D002149:Energy Intake; D007420:Intestine, Large / Q000378:metabolism*; D027806:Lens Plant; D008099:Liver / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012270:Ribosomes / Q000378:metabolism
D004044:Dietary Proteins; D012313:RNA