Subcellular localization of IRS-1 in cell proliferation and differentiation.
The type 1 insulin-like growth factor receptor (IGF-IR) and its docking protein, insulin receptor substrate-1 (IRS-1), play important roles in cell transformation, cell differentiation and aging. IRS-1 and other IRS proteins can, under certain conditions, localize to the nuclei of cells, where they undergo interactions with nuclear and nucleolar proteins. In this study, we confirm and extend these observations, demonstrating that IRS-1 is preferentially nuclear in growing cells. Differentiation and inhibition of ribosomal RNA synthesis cause subcellular redistribution of IRS-1 and other nuclear proteins to the cytoplasm.
Tu
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X
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia 19107, USA.
Wu
A
A
Maiorana
A
A
Baserga
R
R
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D004622:Embryo, Mammalian; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology*; D021381:Protein Transport; D012335:RNA, Ribosomal / Q000235:genetics
C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D010750:Phosphoproteins; D012335:RNA, Ribosomal