title: [Electron microscopic observation of primary cultured laterally spreading tumor cell line].
abstract: OBJECTIVE
  To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary culture.
  
  
  METHODS
  The cells isolated from a rectum LST specimen obtained by endoscopic mucosal resection was primary cultured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocarcinoma and normal mucosa of the rectum.
  
  
  RESULTS
  Scanning and transmission electron microscopes both revealed numerous microvilli covering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes. Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli.
  
  
  CONCLUSIONS
  The cultured LST cells are highly malignant.
authors.affiliation: Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Yu-jing
authors.initials: YJ
authors.lastname: Han
authors.forename: Zhuo-sheng
authors.initials: ZS
authors.lastname: Lai
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Zhi-tao
authors.initials: ZT
authors.lastname: Zhou
authors.forename: Dian-yuan
authors.initials: DY
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000648:ultrastructure*; D045744:Cell Line, Tumor; D006801:Humans; D008854:Microscopy, Electron; D012004:Rectal Neoplasms / Q000648:ultrastructure*