title: Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe.
abstract: Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay was developed for the detection of B. anthracis. After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis.
authors.affiliation: Clinical Research Management, North Royalton, Ohio 44133, USA.
authors.forename: William
authors.initials: W
authors.lastname: Hurtle
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bode
authors.forename: David A
authors.initials: DA
authors.lastname: Kulesh
authors.forename: Rebecca Susan
authors.initials: RS
authors.lastname: Kaplan
authors.forename: Jeff
authors.initials: J
authors.lastname: Garrison
authors.forename: Deanna
authors.initials: D
authors.lastname: Bridge
authors.forename: Michelle
authors.initials: M
authors.lastname: House
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Frye
authors.forename: Bonnie
authors.initials: B
authors.lastname: Loveless
authors.forename: David
authors.initials: D
authors.lastname: Norwood
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D027081:DNA Gyrase / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D027081:DNA Gyrase