Identification of a region within SEL1L protein required for tumour growth inhibition.
The ectopic expression of the entire SEL1L cDNA significantly reduces the proliferate activity and aggressive behavior of the human breast carcinoma cell line MCF-7. However, the mechanism responsible for this biological effect remains unclear. A study was initiated to define those regions within SEL1L conferring such antiproliferative properties. Here we report that the region between amino acid residues 659-794 contains a functionally relevant domain since a deletion mutant impairs SEL1L's ability to suppress tumor cell growth. This region contains the Hrd3 motif, tetratricopeptide (TPR)-like SEL1 repeat, a transmembrane region, and a proline-rich tail.
Cattaneo
Monica
M
Institute for Biomedical Technologies (National Research Council), Milan, Italy.
Canton
Cristina
C
Albertini
Alberto
A
Biunno
Ida
I
D002455:Cell Division / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D014162:Transfection
D018076:DNA, Complementary; D011506:Proteins; C117384:SEL1L protein, human