[Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthma].
OBJECTIVE
To evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma.


METHODS
A model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF), IL-5 in serum, the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope.


RESULTS
IL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF.


CONCLUSIONS
Inhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.
Xu
Feng
F
Medical and Pharmacological, Institute of Yangzhou University, Yangzhou 225001, China.
Zhang
Hong-quan
HQ
D000818:Animals; D017209:Apoptosis; D001249:Asthma / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D001798:Blood Proteins / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006168:Guinea Pigs; D053590:Interleukin 1 Receptor Antagonist Protein; D015848:Interleukin-5 / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D010047:Ovalbumin; D011897:Random Allocation; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012260:Ribonucleases / Q000097:blood / Q000378:metabolism; D012795:Sialoglycoproteins / Q000494:pharmacology*
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D053590:Interleukin 1 Receptor Antagonist Protein; D015848:Interleukin-5; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D010047:Ovalbumin; D012260:Ribonucleases