title: A prolyl endopeptidase-inhibiting antioxidant from Phyllanthus ussurensis.
abstract: A prolyl endopeptidase inhibitor was isolated from the ethyl acetate soluble fraction of Phyllanthus ussurensis. The active compound was identified as an ellagitannin, corilagin. It was shown to non-competitively inhibit prolyl endopeptidase (PEP) with the IC50 value of 1.17x10(-6) microM. The Ki value was 6.70x10(-7) M. Corilagin was less inhibitory to other serine proteases such as chymotrypsin, trypsin, and elastase, indicating that it was relatively a specific inhibitor of PEP. Corilagin also effectively inhibited reactive oxygen species such as hydroxide and superoxide anion radical, hydrogen peroxide, and DPPH. Especially, corilagin showed potent scavenging activity on the superoxide anion radical in the ESR method (IC50 = 3.79x10(-6) M) as well as xanthine oxidase system.
authors.affiliation: Department of Food Technology, College of Agriculture and Life Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Daegu 702-701, Korea.
authors.forename: Shin-Kyo
authors.initials: SK
authors.lastname: Chung
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Nam
authors.forename: So-Young
authors.initials: SY
authors.lastname: Jeon
authors.forename: Sang-In
authors.initials: SI
authors.lastname: Kim
authors.forename: Hee-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Tai Ho
authors.initials: TH
authors.lastname: Chung
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Song
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D029803:Phyllanthus; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases