Effect of richlocaine on proliferative activity of osteoblasts and intracellular calcium content in rats.
We studied the effect of local anesthetic richlocaine on proliferation and intracellular calcium content in cultured osteoblasts from rat parietal bone. In a concentration of 1 mg/ml this drug produced a cytotoxic effect on osteoblasts. In concentrations of 0.01 and 0.001 mg/ml richlocaine in the absence and presence of subtoxic dose of sodium cyanide (0.2 mM) increased the number of osteoblasts by 15.4 and 36.6 or 13.8 and 38.6%, respectively. In a concentration of 1 mg/ml, richlocaine increased the content of cytosolic calcium in osteoblasts by 105%. These effects of richlocaine in low concentrations (0.01 and 0.001 mg/ml) can be related to stimulation of metabolic processes in osteoblasts.
Zadorozhnyi
A V
AV
Kuban State Medical Academy, Krasnodar; Krasnodar Research Center, Russian Academy of Medical Sciences, Krasnodar Region Administration; Russian State Medical University, Moscow.
Popkov
V L
VL
Galenko-Yaroshevskii
P A
PA
Semeikin
A V
AV
D000779:Anesthetics, Local / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival; D002478:Cells, Cultured; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010294:Parietal Bone / Q000166:cytology; D010880:Piperidines / Q000633:toxicity*; D011042:Poisons / Q000494:pharmacology; D051381:Rats; D012966:Sodium Cyanide / Q000494:pharmacology
D000779:Anesthetics, Local; D010880:Piperidines; D011042:Poisons; C493511:richlocaine; D012966:Sodium Cyanide; D002118:Calcium