Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala.
Pichia anomala is an emerging nosocomial pathogen and there is a need for methods that distinguish between different P. anomala strains. In the typing of several clinical as well as non-clinical P. anomala strains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of several P. anomala strains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing of P. anomala strains.
Sutar
Rajeshwari
R
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
David
Joseph K
JK
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
Ganesan
K
K
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
Ghosh
Anup K
AK
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
Singhi
Sunit
S
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
Chakrabarti
Arunaloke
A
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
Bachhawat
Anand K
AK
Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.
D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D021901:DNA, Intergenic / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D005516:Food Microbiology; D016469:Fungemia / Q000382:microbiology*; D014644:Genetic Variation; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000382:microbiology; D010843:Pichia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021901:DNA, Intergenic; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S