title: An association study using AFLP markers and application to a beef cattle breeding population.
abstract: Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University, Kobe 657-8501, Japan. tsuji@ans.kobe-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Sasazaki
authors.forename: H
authors.initials: H
authors.lastname: Mannen
authors.forename: K
authors.initials: K
authors.lastname: Oyama
authors.forename: M
authors.initials: M
authors.lastname: Shojo
authors.forename: F
authors.initials: F
authors.lastname: Mukai
mesh_terms: D000383:Agriculture / Q000379:methods; D000818:Animals; D001824:Body Constitution; D001947:Breeding / Q000379:methods*; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D016002:Discriminant Analysis; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis
chemical_list: D017931:DNA Primers