Origins of protein stability revealed by comparing crystal structures of TATA binding proteins.
The crystal structure of TATA binding protein (TBP) from a mesothermophilic archaeon, Sulfolobus acidocaldarius, has been determined at a resolution of 2.0 A with an R factor of 20.9%. By comparing this structure with the structures of TBPs from a hyperthermophilic archaeon and mesophilic eukaryotes, as well as by comparing amino acid sequences of TBPs from archaea, covering a wide range of optimum growth temperatures, two significant determinants of the stability of TBP have been identified: increasing the interior hydrophobicity by interaction between three residues, Val, Leu, and Ile, with further differentiation of the surface, and increasing its hydrophilicity and raising the cost of unfolding. These findings suggest directions along which the stability of TBP can be engineered.
Koike
Hideaki
H
National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Center 6-10, Higashi 1-1-1, Tsukuba 305-8566, Japan.
Kawashima-Ohya
Yoshie
Y
Yamasaki
Tomoko
T
Clowney
Lester
L
Katsuya
Yoshio
Y
Suzuki
Masashi
M
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000235:genetics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016945:Sulfolobus acidocaldarius / Q000737:chemistry* / Q000235:genetics; D035181:TATA-Box Binding Protein / Q000737:chemistry*; D013696:Temperature
D035181:TATA-Box Binding Protein