Loss of CCAAT/enhancer binding protein delta promotes chromosomal instability.
The transcription factor CCAAT/enhancer binding protein delta (Cebpd, also known as C/EBPdelta, CRP3, CELF, NF-IL6beta) is implicated in diverse cellular functions such as the acute phase response, adipocyte differentiation, learning and memory, and mammary epithelial cell growth control. Here, we report that lack of Cebpd causes genomic instability and centrosome amplifications in primary embryonic fibroblasts derived from 129S1 mice. Upon spontaneous immortalization, Cebpd-deficient fibroblasts acquire transformed features such as impaired contact inhibition and reduced serum dependence. These data identify a novel role for Cebpd in the maintenance of chromosomal stability and suggest a potential tumor suppressor function in vivo.
Huang
A-Mei
AM
Regulation of Cell Growth Laboratory, PO Box B, Frederick, MD 21702, USA.
Montagna
Cristina
C
Sharan
Shikha
S
Ni
Yajun
Y
Ried
Thomas
T
Sterneck
Esta
E
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D051743:CCAAT-Enhancer-Binding Protein-delta; D022762:CCAAT-Enhancer-Binding Proteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D043171:Chromosomal Instability; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D007621:Karyotyping; D051379:Mice / Q000196:embryology; D018345:Mice, Knockout / Q000196:embryology; D041681:NIH 3T3 Cells; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*
D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins; C497253:Cebpd protein, mouse; D014157:Transcription Factors; D051743:CCAAT-Enhancer-Binding Protein-delta