title: Time course of efferent fiber and spiral ganglion cell degeneration following complete hair cell loss in the chinchilla.
abstract: Ethacrynic acid (EA) is known to interact with aminoglycoside antibiotics such as gentamicin (GM). In the chinchilla, co-administration of GM and EA can produce hair cell lesions ranging from a small loss of outer hair cells (OHCs) in the base of the cochlea to complete destruction of all hair cells, depending on dosing parameters. Although hair cell loss has been characterized, little is known about the fate of efferent fibers or spiral ganglion neurons (SGNs) in this model. To study the time course of efferent fiber and SGN loss, chinchillas were injected with GM (125 mg/kg IM) followed immediately by EA (40 mg/kg IV). Estimates of efferent fiber loss and density changes were made after 3 days or 1, 2, 3, or 4 weeks of survival. Estimates of SGN loss and density changes were made after 15 days or 1, 2, 4, or 6 months of survival. Cochlear function was rapidly abolished and all cochlear hair cells were missing within 24 h after treatment. Inner hair cells (IHCs) in the middle turn of the cochlea died earlier than cells in the apex or base, and OHCs in Rows 1 and 2 died earlier than OHCs in Row 3. Degeneration of efferent nerve fibers began 3-7 days post-injection, versus 15-30 days for SGNs, and the loss of efferent fibers was essentially complete within 1 month, versus 2-4 months for SGNs. The rapid time course of efferent fiber and SGN loss in the chinchilla may make it a practical model for studying mechanisms of neural loss and survival in the mammalian inner ear.
authors.affiliation: Center for Hearing and Deafness, University at Buffalo, 215 Parker Hall, Buffalo, NY 14214, USA. mcfadden@buffalo.edu
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McFadden
authors.forename: Dalian
authors.initials: D
authors.lastname: Ding
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jiang
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Salvi
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D002682:Chinchilla; D003055:Cochlear Microphonic Potentials / Q000187:drug effects; D004525:Efferent Pathways / Q000473:pathology*; D004976:Ethacrynic Acid / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006198:Hair Cells, Auditory / Q000473:pathology*; D006319:Hearing Loss, Sensorineural / Q000139:chemically induced / Q000503:physiopathology; D007150:Immunohistochemistry; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology*; D009925:Organ of Corti / Q000166:cytology / Q000187:drug effects; D012835:Silver Nitrate / Q000378:metabolism; D013136:Spiral Ganglion / Q000187:drug effects / Q000473:pathology*; D013997:Time Factors
chemical_list: D005839:Gentamicins; D012835:Silver Nitrate; D000110:Acetylcholinesterase; D004976:Ethacrynic Acid