title: Effect of phytosterols and phytostanols on the solubilization of cholesterol by dietary mixed micelles: an in vitro study.
abstract: The effect of a plant sterol, beta-sitosterol (SI), and a plant stanol, sitostanol (SS), on the solubilization of cholesterol (CH) by model dietary mixed micelles was examined under in vitro conditions with the use of gas chromatography, isothermal titration calorimetry, NMR spectroscopy and cryogenic transmission electron microscopy techniques. Free SI and SS were shown to reduce the concentration of CH in dietary mixed micelles via a dynamic competition mechanism. CH, SI and SS affect the microstructure of lipid vesicles and influence the process of amphiphilic self-assembly of nutrients in the gut with the formation of dietary mixed micelles in a similar manner. Therefore, substitution of CH by phytosterols and phytostanols in the diet does not lead to the notable changes in the mechanism of dietary mixed micelle formation and does not affect the process of the intestinal transport of nutrients and drugs via the micellar diffusion mechanism. Our experimental findings demonstrate that the introduction of plant sterols and plant stanols into the diet is clearly beneficial for the reduction of the intestinal uptake of cholesterol. Due to the limited capacity of dietary mixed micelles to embody hydrophobic sterol/stanol molecules, the micellar concentration of cholesterol is reduced and hence, its transport towards the intestinal brush border membrane decreases.
authors.affiliation: Foods Research Centre, Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands. sergey.melnikov@unilever.com
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Mel'nikov
authors.forename: Jack W M
authors.initials: JW
authors.lastname: Seijen ten Hoorn
authors.forename: Astrid P A M
authors.initials: AP
authors.lastname: Eijkelenboom
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry; D002151:Calorimetry / Q000379:methods; D002784:Cholesterol / Q000737:chemistry*; D002791:Cholesterol, Dietary / Q000493:pharmacokinetics; D002849:Chromatography, Gas; D020285:Cryoelectron Microscopy; D000960:Hypolipidemic Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D019301:Oleic Acid / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D012995:Solubility; D013816:Thermodynamics
chemical_list: D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D000960:Hypolipidemic Agents; D008823:Micelles; D010713:Phosphatidylcholines; D012855:Sitosterols; D019301:Oleic Acid; C025473:gamma-sitosterol; D002784:Cholesterol; C021255:stigmastanol