[Improvement of the method for the indication of the causative agent of anthrax].
A combined method for the indication of the causative agent of anthrax (Bacillus anthracis), including the preparation of the material to be tested, the exposure of the magneto-imunosorbent in the sample, cultivation in selective medium, DNA extraction with subsequent testing in the polymerase chain reaction with primers to genes cap, pag and chromosomal sequence Ba813, the registration and interpretation of results, has been developed. All determinations, including the preparation of samples, last not more than 6 hours. The indication of B. anthracis by the proposed method makes it possible not only to confirm its presence in the sample Under test, but also to evaluate its epidemic potential.
Abgarian
A G
AG
Research Institute for Plague Control, Stavropol.
Eremenko
E I
EI
Efremenko
V I
VI
Afans'ev
E N
EN
Zharnikova
I V
IV
D000881:Anthrax / Q000209:etiology / Q000382:microbiology*; D001408:Bacillus anthracis / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction; D012641:Selection, Genetic
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial