[Photodynamic inactivation of porphyrin sensitized T7 phages: efficiency and mechanism of action].
We investigated the efficiency and the mechanism of action of two glycoconjugated tetraphenyl porphyrins in their photoreaction with T7 bacteriophage. Both types of porphyrins sensitized the photoinactivation of T7, but the slopes of inactivation kinetics were markedly different. Our result suggests that both type I and type II reaction play a role in the virus inactivation. Optical melting studies revealed structural changes in the protein part but not in the DNA of the photo-chemically treated nucleoprotein complex. Polymerase chain reaction (PCR) analysis failed to demonstrate any DNA damage.
Egyeki
Marianna
M
Semmelweis Egyetem, Biofizikai és Sugárbiológiai Intézet, Budapest, POB 263.-1444.
Turóczy
Gergely
G
Tóth
Katalin
K
Fekete
Andrea
A
Maillard
Philippe
P
Csík
Gabriella
G
D017123:Bacteriophage T7 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D001483:Base Sequence; D017931:DNA Primers; D010084:Oxidation-Reduction; D010778:Photochemotherapy; D016133:Polymerase Chain Reaction; D011166:Porphyrins / Q000494:pharmacology*; D038301:Virus Inactivation / Q000187:drug effects / Q000528:radiation effects
D017931:DNA Primers; D011166:Porphyrins