Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.
The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed.
Sims
Andrew H
AH
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Robson
Geoffrey D
GD
Hoyle
David C
DC
Oliver
Stephen G
SG
Turner
Geoffrey
G
Prade
Rolf A
RA
Russell
Hugh H
HH
Dunn-Coleman
Nigel S
NS
Gent
Manda E
ME
D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000302:isolation & purification; D000431:Ethanol / Q000378:metabolism; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D005943:Gluconeogenesis / Q000235:genetics; D005947:Glucose / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results
D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D006038:Glyoxylates; D000431:Ethanol; D005947:Glucose