title: Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.
abstract: An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
authors.affiliation: Applied Microbiology, Lund Institute of Technology, Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Petra
authors.initials: P
authors.lastname: Wolffs
authors.forename: Halfdan
authors.initials: H
authors.lastname: Grage
authors.forename: Oskar
authors.initials: O
authors.lastname: Hagberg
authors.forename: Peter
authors.initials: P
authors.lastname: Rådström
mesh_terms: D002021:Buffers; D004259:DNA-Directed DNA Polymerase / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011336:Probability; D015203:Reproducibility of Results
chemical_list: D002021:Buffers; D004259:DNA-Directed DNA Polymerase