title: Experimental Corynebacterium pseudotuberculosis mastitis in dairy cows.
abstract: The inoculation of 2000 colony-forming units of Corynebacterium pseudotuberculosis into one teat canal of each of three cows resulted in severe, chronic, pyogranulomatous mastitis. Within three days the cows had a reduced haematocrit, haemoglobin concentration and red cell count. The anaemia was initially normocytic, normochromic and non-regenerative, and was associated with a brief peak of neutrophilia; a regenerative response became evident two to three weeks later. Clinical signs of mastitis appeared seven to 14 days after the inoculation, with a peak of high fever, more severe anaemia, a second peak of neutrophilia and the complete cessation of milk production from all quarters; extensive and severe pyogranulomatous mastitis developed in the inoculated quarters. No other lesions were detected postmortem, and C pseudotuberculosis was cultured from the affected quarters but not from the supramammary lymph nodes and viscera.
authors.affiliation: School of Veterinary Medicine, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Aroch
authors.forename: A
authors.initials: A
authors.lastname: Harmelin
authors.forename: A
authors.initials: A
authors.lastname: Saran
authors.forename: D
authors.initials: D
authors.lastname: Levin
authors.forename: N Y
authors.initials: NY
authors.lastname: Shpigel
mesh_terms: D000740:Anemia / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D003354:Corynebacterium Infections / Q000097:blood / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000472:pathogenicity*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000473:pathology; D008414:Mastitis, Bovine / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D008892:Milk / Q000166:cytology / Q000382:microbiology; D009504:Neutrophils / Q000473:pathology