title: Localization of the 31-amino-acid endothelin-1 in hamster tissue.
abstract: Endothelin (ET)-1(1-31) is a novel vasoconstrictor peptide produced by human mast cell chymase, which selectively cleaves big ET-1 at the Try(31)-Gly(32) bond. We investigated the localization of ET-1(1-31) in various hamster tissues by immunohistochemistry and compared it to the distribution of ET-1(1-21). We found that the localization and amount of ET-1(1-31) were different from those of ET-1(1-21) in each tissue. ET-1(1-31)-like immunoreactivities (IR) in the heart, lung, and adrenal gland were observed in the same areas as ET-1(1-21) but were significantly weaker, suggesting that ET-1(1-31) might play a role only in mast cell/chymase-related pathological conditions in these tissues. In the liver, ET-1(1-31)-like IR was strongly detected in Kupffer cells where ET-1(1-21)-like IR was seen more weakly. In the kidney, ET-1(1-31)-like IR was slightly higher than ET-1(1-21). These results suggest that ET-1(1-31) might have physiological roles distinct from those of ET-1(1-21) in some hamster tissues.
authors.affiliation: Department of Nutrition, School of Medicine, Tokushima University, Tokushima, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan
authors.forename: Sae
authors.initials: S
authors.lastname: Kakui
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Mawatari
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Niwa
authors.forename: Naomi
authors.initials: N
authors.lastname: Tanoue
authors.forename: Nagakatsu
authors.initials: N
authors.lastname: Harada
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Izumi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakaya
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D053818:Chymases; D006224:Cricetinae; D019332:Endothelin-1 / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism*; D007120:Immunochemistry; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D019332:Endothelin-1; D010446:Peptide Fragments; D010455:Peptides; C114932:endothelin-1 (1-31); D012697:Serine Endopeptidases; D053818:Chymases