Interaction of plasminogen activator inhibitor-2 and proteasome subunit, beta type 1.
The apoptosis protection by plasminogen activator inhibitor -2(PAI-2) is dependent on a 33 amino acids fragment between helix C and D of PAI-2 which is probably may be due to the interaction of PAI-2 with unknown intracellular proteins. In this study we used the fragment between helix C and D of PAI-2 as bait to screen a HeLa cells cDNA library constructed during apoptosis in a yeast two-hybrid system and retrieved a clone that encodes 241 amino acids of proteasome (prosome, macropain) subunit, beta type 1(PSMbeta1) which plays important roles in NF-kappaB activation. GST-pulldown experiments confirmed the interaction between PAI-2 and PSMB1 in vitro. These data suggest that the antiapoptosis activity of PAI-2 is probably related to its interaction with PSMbeta1.
Fan
Jing
J
Department of Molecular Genetics, Shanghai Medical School of Fudan University, Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai 200032, China. yszhu@shmu.edu.cn
Zhang
Yu-Qing
YQ
Li
Ping
P
Hou
Min
M
Tan
Li
L
Wang
Xia
X
Zhu
Yun-Song
YS
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology*; D001665:Binding Sites; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000378:metabolism*; D017396:Plasminogen Activator Inhibitor 2 / Q000737:chemistry* / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D009097:Multienzyme Complexes; D017396:Plasminogen Activator Inhibitor 2; D021122:Protein Subunits; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex