title: Resistance to pseudorabies virus infection in transformed cell lines expressing a soluble form of porcine herpesvirus entry mediator C.
abstract: Porcine herpesvirus entry mediator C (HveC) is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine HveC mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) and bovine herpesvirus type 1 (BHV-1). In order to assess the antiviral potential of a soluble form of porcine HveC, Vero cells were transformed with the chimeric gene expressing a fusion protein (PHveCIg) consisting of an extracellular domain of porcine HveC and the Fc portion of human IgG1. The transformed cell lines expressing PHveCIg showed marked resistance to PRV infection. Resistance to infection by other alphaherpesviruses (HSV-1 and BHV-1) was also observed in the transformed cell line. The present results demonstrate that a soluble form of porcine HveC is able to exert a significant antiviral effect against pseudorabies virus and other alphaherpesvirus infection in vitro.
authors.affiliation: Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Etsuro
authors.initials: E
authors.lastname: Ono
authors.affiliation: Sankyo Labo Service Corporation, Tokyo 132-0023, Japan.|Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Amagai
authors.affiliation: Gene Techno Science, Sapporo 062-8517, Japan.|Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Yoshino
authors.affiliation: Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taharaguchi
authors.affiliation: Division of Molecular Immunology, Research Section of Molecular Pathogenesis, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Inobe
authors.affiliation: Division of Molecular Immunology, Research Section of Molecular Pathogenesis, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Uede
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D007242:Herpesvirus 1, Bovine / Q000472:pathogenicity; D018259:Herpesvirus 1, Human / Q000472:pathogenicity; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000472:pathogenicity*; D049673:History, 20th Century; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry; D011557:Pseudorabies / Q000517:prevention & control*; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000378:metabolism*; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D013552:Swine; D014709:Vero Cells; D010948:Viral Plaque Assay
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human