A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver.
We previously reported a new in vivo model named as "GFP/CCl(4) model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl(4) induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver.
Yamamoto
Naoki
N
Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, Minami Kogushi 1-1-1, Ube, Yamaguchi 755-8505, Japan.
Terai
Shuji
S
Ohata
Shinya
S
Watanabe
Tomomi
T
Omori
Kaoru
K
Shinoda
Koh
K
Miyamoto
Koji
K
Katada
Toshiaki
T
Sakaida
Isao
I
Nishina
Hiroshi
H
Okita
Kiwamu
K
D000418:Albumins / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000145:classification* / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride / Q000633:toxicity; D002454:Cell Differentiation; D002469:Cell Separation; D005260:Female; D005333:Fetus / Q000166:cytology / Q000276:immunology; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000276:immunology; D022781:Hepatocytes / Q000166:cytology / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008099:Liver / Q000276:immunology* / Q000293:injuries* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics
D000418:Albumins; D000911:Antibodies, Monoclonal; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D002251:Carbon Tetrachloride; D017493:Leukocyte Common Antigens