title: [Evaluation of the heterogeneous immunoassay (ACMIA) for the measurement of blood cyclosporin on the Behring dimension RXL clinical chemistry analyzer].
abstract: We propose an evaluation of a new heterogeneous immunoassay of cyclosporin on RXL HM Dimension (Dade Behring) for therapeutic cyclosporin monitoring in whole-blood patients transplant. The pretreatment step is performed automatically into the apparatus while it is a manual step with EMIT. Linearity, intra- and inter-day precision, limit of quantification, precision and accuracy of dilution steps and stability into the equipment were studied. We realized the comparison between ACMIA and EMIT methods on whole-blood patients transplant recipients. Heterogeneous immunoassay showed a good linearity between 0 and 500 ng/mL, intra- and inter-day precision with coefficient of variation inferior to 7.2%. We observed reproducible and accurate dilutions of high concentrations (500 to 2,000 ng/mL). The correlation with EMIT technique was correct for different type of transplant (n=55).
authors.affiliation: Laboratoire de pharmacologie et toxicologie, CHU Henri Mondor, 51, avenue du Maréchal de Lattre de Tassigny, 94010 Créteil.
authors.forename: K
authors.initials: K
authors.lastname: Morand
authors.forename: E
authors.initials: E
authors.lastname: Huet
authors.forename: B
authors.initials: B
authors.lastname: Blanchet
authors.forename: A
authors.initials: A
authors.lastname: Astier
authors.forename: A
authors.initials: A
authors.lastname: Hulin
mesh_terms: D016572:Cyclosporine / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods
chemical_list: D016572:Cyclosporine