[Transduction efficiency of recombinant adeno-associated virus 2 in human umbilical cord blood CD34+ hematopoietic stem/progenitor cells].
To investigate the transduction efficiency of recombinant adeno-associated virus 2 (rAAV-2) in human umbilical cord blood CD34(+) hematopoietic stem/progenitor cells, the CD34(+) cells sorted by the method of magnetic cell sorting from human cord blood were infected with the rAAV-2 expressing the green fluorescent protein (GFP) gene. After transduction for 19 hours, the expression of GFP was detected under fluorescence microscope. The results showed that 43% CD34(+) cells expressed the GFP gene at a multiplicity of infection of 2 x 10(5). It is concluded that the rAAV-2 can transduce human cord blood CD34(+) hematopoietic stem/progenitor cells efficiently.
Chen
Yan
Y
Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
Chen
Fang-Ping
FP
Peng
Jian-Qiang
JQ
Wu
Xiao-Bing
XB
Wu
Xin-Hua
XH
Fu
Gan
G
D018952:Antigens, CD34 / Q000032:analysis*; D000229:Dependovirus / Q000235:genetics*; D005312:Fetal Blood / Q000166:cytology*; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D018952:Antigens, CD34; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins