title: Establishment of transgenic mice carrying gene encoding human zinc finger protein 191.
abstract: OBJECTIVE
  Human zinc finger protein 191 (ZNF191) was cloned and characterized as a Kruppel-like transcription factor, which might be relevant to many diseases such as liver cancer, neuropsychiatric and cardiovascular diseases. Although progress has been made recently, the biological function of ZNF191 remains largely unidentified. The aim of this study was to establish a ZNF 191 transgenic mouse model, which would promote the functional study of ZNF191.
  
  
  METHODS
  Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. The offsprings were identified by PCR and Southern blot analysis. ZNF 191 gene expression was analyzed by RT-PCR. Transgenic founder mice were used to establish transgenic mouse lineages. The first generation (F1) and the second generation (F2) mice were identified by PCR analysis. Ten-week transgenic mice were used for pathological examination.
  
  
  RESULTS
  Four mice were identified as carrying copies of ZNF191 gene. The results of RT-PCR showed that ZNF 191 gene was expressed in the liver, testis and brain in one of the transgenic mouse lineages. Genetic analysis of transgenic mice demonstrated that ZNF 191 gene was integrated into the chromosome at a single site and could be transmitted stably. Pathological analysis showed that the expression of ZNF 191 did not cause obvious pathological changes in multiple tissues of transgenic mice.
  
  
  CONCLUSIONS
  ZNF 191 transgenic mouse model would facilitate the investigation of biological functions of ZNF191 in vivo.
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Shui-Liang
authors.initials: SL
authors.lastname: Wang
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Gong
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.forename: Bao-Yu
authors.initials: BY
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.forename: Zhu-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Fu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D011247:Pregnancy; D014781:Viscera / Q000473:pathology / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; C457300:ZNF24 protein, human