Sepsis induces DNA fragmentation in rat skeletal muscle.
Recent studies have demonstrated the activation of skeletal muscle DNA fragmentation in some catabolic conditions. In an attempt to elucidate if sepsis (a catabolic state) was also associated with muscle apoptosis, sepsis was induced by cecal ligation and puncture, and the results clearly show an induction of DNA fragmentation in gastrocnemius muscle following the induction of the septic state. Administration of rolipram (an inhibitor of tumour necrosis factor-a (TNF-alpha) synthesis) to septic rats clearly prevented the increased DNA fragmentation, suggesting that TNF-alpha is involved in the activation of the apoptotic events in septic rat skeletal muscle.
Almendro
Vanessa
V
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Barcelona, Spain.
Carbó
Neus
N
Busquets
Sílvia
S
Figueras
Maite
M
Tessitore
Luciana
L
López-Soriano
Francisco J
FJ
Argilés
Josep M
JM
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053938:DNA Fragmentation / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha