title: Genomic expression pattern in Saccharomyces cerevisiae cells in response to high hydrostatic pressure.
abstract: Gene expression patterns in response to hydrostatic pressure were determined by whole genome microarray hybridization. Functional classification of the 274 genes affected by pressure treatment of 200 MPa for 30 min revealed a stress response expression profile. The majority of the >2-fold upregulated genes were involved in stress defense and carbohydrate metabolism while most of the repressed ones were in cell cycle progression and protein synthesis categories. Furthermore, uncharacterized genes were among the 10 highest expressed sequences and represented 45% of the total upregulated genes. The results of this study revealed a hydrostatic pressure-specific stress response pattern and suggested interesting information about the mechanisms involved in adaptation of cells to a high-pressure environment.
authors.affiliation: Departamento de Ciências Fisiológicas, CBM, UFES, 29090-040, ES, Vitória, Brazil. pat@npd.ufes.br
authors.forename: Patricia M B
authors.initials: PM
authors.lastname: Fernandes
authors.forename: Tatiana
authors.initials: T
authors.lastname: Domitrovic
authors.forename: Camilla M
authors.initials: CM
authors.lastname: Kao
authors.forename: Eleonora
authors.initials: E
authors.lastname: Kurtenbach
mesh_terms: D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D016681:Genome, Fungal; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006874:Hydrostatic Pressure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger