title: [In vitro experimental study of gene therapy for ovarian cancer with thymidine kinase gene of herpes simplex virus mediated by a non-viral GE7 delivery system].
abstract: OBJECTIVE
  To investigate gene transfer efficiency of a novel target non-viral vector GE7 and effects of herpes simplex virus thymidine kinase (HSV(1)-tk)/ganciclovir (GCV) mediated by it in vitro.
  
  
  METHODS
  The epidermal growth factor receptor (EGF-R) target gene delivery system GE7 was constructed. Human ovarian cancer cell line CAOV3 was transfected in vitro with beta-galactosidase (beta-gal) as reporter gene and HSV(1)-tk gene as therapeutic gene using this gene delivery system. By means of the assay of X-gal staining, Northern blotting, cell growth-inhibiting curve and so on, the transferring efficiency of exogenous genes and killing effects are observed.
  
  
  RESULTS
  It showed that gene transfer efficiency is over 80%. When 10 mg/L GCV was put into ovarian cells transfected with HSV(1)-tk gene, 95% of cells were killed, and the apoptosis ratio reached up to 30.
  
  
  CONCLUSIONS
  The GE7 gene delivery system is an effective and safe delivery system. GE7/HSV(1)-tk/GCV therapeutic gene system is appraising for ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Cong-jian
authors.initials: CJ
authors.lastname: Xu
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Jin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Fei-han
authors.initials: FH
authors.lastname: Dai
authors.forename: Jun-song
authors.initials: JS
authors.lastname: Han
authors.forename: Pei-kun
authors.initials: PK
authors.lastname: Tian
authors.forename: Jian-ren
authors.initials: JR
authors.lastname: Gu
mesh_terms: D002455:Cell Division; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D015774:Ganciclovir / Q000627:therapeutic use; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D010051:Ovarian Neoplasms / Q000628:therapy*; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D013937:Thymidine Kinase; D066246:ErbB Receptors; D015774:Ganciclovir