Rapid and efficient isolation of highly specific antibodies from an antiserum against a pool of proteins.
Antibodies with desired specificity to proteins of interest provide important and versatile tools for detecting and localizing specific proteins in organisms. With the rapidly increasing number of genes cloned, the demand for antibodies to the gene products is increasing greatly. We developed a procedure to isolate highly specific antibodies to an insect intestinal mucin (IIM) from a polyclonal antiserum, which served as a "library of antibodies," by using an E. coli lysate of the IIM cDNA clone. This procedure allows rapid and efficient isolation of target protein specific antibodies from a polyclonal antiserum made against a pool of antigens.
Wang
P
P
Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456, USA. pw15@cornell.edu
Granados
R R
RR
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D015153:Blotting, Western; D004926:Escherichia coli; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology; D008856:Microscopy, Fluorescence; D009077:Mucins / Q000276:immunology; D011506:Proteins / Q000276:immunology*
D000906:Antibodies; D007106:Immune Sera; D019476:Insect Proteins; D009077:Mucins; D011506:Proteins