Glucose biosensor based on the microcantilever.
Diagnosis and management of diabetes require quantitative and selective detection of blood glucose levels. We report a technique for micromechanical detection of biologically relevant glucose concentrations by immobilization of glucose oxidase (GOx) onto a microcantilever surface. Microfabricated cantilevers have traditionally found utility in atomic force microscope imaging. During the past decade, however, microcantilevers have been increasingly used as transducers in chemical-sensing systems. This paper describes the combination of this technology with enzyme specificity to construct a highly selective glucose biosensor. The enzyme-functionalized microcantilever undergoes bending due to a change in surface stress induced by the reaction between glucose in solution and the GOx immobilized on the cantilever surface. Experiments were carried out under flow conditions. The common interferences for glucose detection in other detection schemes have been tested and have shown to have no effect on the measurement of blood glucose level by this technique.
Pei
Jianhong
J
Life Science Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6123, USA.
Tian
Fang
F
Thundat
Thomas
T
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003920:Diabetes Mellitus / Q000175:diagnosis / Q000628:therapy; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D007834:Lasers; D008832:Microchemistry; D044085:Microfluidics; D018625:Microscopy, Atomic Force; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D014159:Transducers
D005949:Glucose Oxidase; D005947:Glucose