Nanoparticles as a vaccine adjuvant of anti-idiotypic antibody against schistosomiasis.
BACKGROUND
The development of new adjuvants for human use has been the focus of attention. This study's aim is to explore the possibility of using nanoparticle Ca nanoparticles (CA) as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis and its protective mechanisms.


METHODS
Nanoparticle CA-NP30 conjugate (CA-NP30) was fabricated. BALB/c mice were immunized actively with CA-NP30 to evaluate its effects of protective immunity on mice. The serum levels of specific IgG, IgG1 and IgG2a antibodies against NP30 and the concentrations of IFN-gamma and IL-4 in supernatant of splenocytes were determined via ELISA.


RESULTS
Nanoparticle CA could enhance significantly the protective immunity of NP30 against infection of Schistosoma japonicum and the worm reduction rose from 36.0% (NP30 alone) to 52.6%. The serum levels of specific IgG, IgG1 and IgG2a antibodies against NP30 increased remarkably, as compared with those of the group immunized with NP30 alone. The concentration of IFN-gamma in supernatant of splenocyte was drastically elevated [the groups immunized with CA-NP30 and NP30 alone were (493.80 +/- 400.74) pg/ml and (39.03 +/- 39.58) pg/ml, respectively], but the concentration of IL-4 showed no significant difference from that of NP30 alone [(27.94 +/- 9.84) pg/ml vs (27.28 +/- 14.44) pg/ml].


CONCLUSIONS
Nanoparticle CA could act as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis. The mechanism could be that CA-NP30 enhances humoral and cellular immune responses in mice.
Feng
Zhen-qing
ZQ
Department of Pathology, Nanjing Medical University, Nanjing 210029, China. bljysh@njmu.edu.cn
Zhong
Shi-gen
SG
Li
Yu-hua
YH
Li
Yun-qian
YQ
Qiu
Zhen-ning
ZN
Wang
Zhu-ming
ZM
Li
Jun
J
Dong
Li
L
Guan
Xiao-hong
XH
D000276:Adjuvants, Immunologic; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000909:Antibodies, Helminth / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology; D012552:Schistosomiasis / Q000517:prevention & control*; D014612:Vaccines
D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D014612:Vaccines