title: The mechanism of Polydatin in shock treatment.
abstract: Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM-1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC-EC adhesion, increase [Ca2+]i in MC with enhancement of MC contraction extent, activate KATP channels of VSMC, and decrease pHi value and [Ca2+]i of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.
authors.affiliation: Department of Pathophysiology, First Military Medical University, Guangzhou 510515, China. zhaoks@fimmu.com
authors.forename: Ke-Seng
authors.initials: KS
authors.lastname: Zhao
authors.forename: Chunhua
authors.initials: C
authors.lastname: Jin
authors.forename: Xuliang
authors.initials: X
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Wen Sheng
authors.initials: WS
authors.lastname: Yan
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Huang
authors.forename: Wenhong
authors.initials: W
authors.lastname: Kan
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008833:Microcirculation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D018408:Patch-Clamp Techniques; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D012771:Shock, Hemorrhagic / Q000188:drug therapy*; D012772:Shock, Septic / Q000188:drug therapy / Q000503:physiopathology; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D005960:Glucosides; D015221:Potassium Channels; D013267:Stilbenes; D018799:Intercellular Adhesion Molecule-1; D014700:Verapamil; C058229:polydatin