title: Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase.
abstract: Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-ATP synthases from Escherichia coli into liposomes and measured single-molecule fluorescence resonance energy transfer (FRET) during ATP synthesis and hydrolysis. The gamma subunit rotates stepwise during proton transport-powered ATP synthesis, showing three distinct distances to the b subunits in repeating sequences. The average durations of these steps correspond to catalytic turnover times upon ATP synthesis as well as ATP hydrolysis. The direction of rotation during ATP synthesis is opposite to that of ATP hydrolysis.
authors.affiliation: Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 23 a, 79104 Freiburg, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Diez
authors.forename: Boris
authors.initials: B
authors.lastname: Zimmermann
authors.forename: Michael
authors.initials: M
authors.lastname: Börsch
authors.forename: Marcelle
authors.initials: M
authors.lastname: König
authors.forename: Enno
authors.initials: E
authors.lastname: Schweinberger
authors.forename: Stefan
authors.initials: S
authors.lastname: Steigmiller
authors.forename: Rolf
authors.initials: R
authors.lastname: Reuter
authors.forename: Suren
authors.initials: S
authors.lastname: Felekyan
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Kudryavtsev
authors.forename: Claus A M
authors.initials: CA
authors.lastname: Seidel
authors.forename: Peter
authors.initials: P
authors.lastname: Gräber
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism; D002384:Catalysis; D004735:Energy Transfer; D005453:Fluorescence; D006868:Hydrolysis; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011522:Protons
chemical_list: D011522:Protons; D000266:Adenylyl Imidodiphosphate; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases