title: Cloning and characterization of acetohydroxyacid synthase from Bacillus stearothermophilus.
abstract: Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105 Israel.
authors.forename: Iris
authors.initials: I
authors.lastname: Porat
authors.forename: Michael
authors.initials: M
authors.lastname: Vinogradov
authors.forename: Maria
authors.initials: M
authors.lastname: Vyazmensky
authors.forename: Chung-Dar
authors.initials: CD
authors.lastname: Lu
authors.forename: David M
authors.initials: DM
authors.lastname: Chipman
authors.forename: Ahmed T
authors.initials: AT
authors.lastname: Abdelal
authors.forename: Ze'ev
authors.initials: Z
authors.lastname: Barak
mesh_terms: D000095:Acetolactate Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D008969:Molecular Sequence Data; D021122:Protein Subunits; D014633:Valine / Q000494:pharmacology
chemical_list: D021122:Protein Subunits; D000095:Acetolactate Synthase; D014633:Valine