Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain.
Both late-gestation and adult human forebrain contain large numbers of oligodendrocyte progenitor cells (OPCs). These cells may be identified by their A2B5(+)PSA-NCAM(-) phenotype (positive for the early oligodendrocyte marker A2B5 and negative for the polysialylated neural cell adhesion molecule). We used dual-color fluorescence-activated cell sorting (FACS) to extract OPCs from 21- to 23-week-old fetal human forebrain, and A2B5 selection to extract these cells from adult white matter. When xenografted to the forebrains of newborn shiverer mice, fetal OPCs dispersed throughout the white matter and developed into oligodendrocytes and astrocytes. By 12 weeks, the host brains showed extensive myelin production, compaction and axonal myelination. Isolates of OPCs derived from adult human white matter also myelinated shiverer mouse brain, but much more rapidly than their fetal counterparts, achieving widespread and dense myelin basic protein (MBP) expression by 4 weeks after grafting. Adult OPCs generated oligodendrocytes more efficiently than fetal OPCs, and ensheathed more host axons per donor cell than fetal cells. Both fetal and adult OPC phenotypes mediated the extensive and robust myelination of congenitally dysmyelinated host brain, although their differences suggested their use for different disease targets.
Windrem
Martha S
MS
Department of Neurology and Neuroscience, Cornell University Medical College, 1300 York Ave., New York, New York 10021, USA.
Nunes
Marta C
MC
Rashbaum
William K
WK
Schwartz
Theodore H
TH
Goodman
Robert A
RA
McKhann
Guy
G
Roy
Neeta S
NS
Goldman
Steven A
SA
D000328:Adult; D000818:Animals; D003711:Demyelinating Diseases / Q000473:pathology / Q000628:therapy*; D005333:Fetus / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D004676:Myelin Basic Protein / Q000096:biosynthesis*; D009836:Oligodendroglia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*
D004676:Myelin Basic Protein