A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor.
A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIHml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed.
Garden
Steven R
SR
Department of Plant, Soil Science, School of Biological Sciences, Cruickshank Building, University of Aberdeen, Aberdeen AB24 3UU, UK.
Doellgast
George J
GJ
Killham
Kenneth S
KS
Strachan
Norval J C
NJ
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001780:Blood Coagulation Tests / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013917:Thrombin / Q000032:analysis*
D013917:Thrombin