title: The use of mass spectrometry in genomics.
abstract: Recent advances in the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) now permit the near routine analysis of oligonucleotides and intact nucleic acids. These developments have led to the use of mass spectrometry (MS) as a detection platform for genomics studies. Among the various uses of mass spectrometry in genomics, applications focused on the characterization of single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs) are particularly well-suited to MALDI or ESI-based analysis. It is predicted that continued developments in methodology and instrumentation will further improve the capabilities of mass spectrometry for nucleic acid analysis.
authors.affiliation: Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, PO Box 210172, Cincinnati, OH 45221-0172, USA.
authors.forename: Zhaojing
authors.initials: Z
authors.lastname: Meng
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Simmons-Willis
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Limbach
mesh_terms: D001483:Base Sequence; D023281:Genomics / Q000379:methods* / Q000639:trends; D013058:Mass Spectrometry / Q000379:methods / Q000639:trends; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000639:trends; D017423:Sequence Analysis, RNA / Q000379:methods* / Q000639:trends; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000639:trends; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000639:trends
chemical_list: D009696:Nucleic Acids