title: Ectopic localization of putative AII amacrine cells in the outer plexiform layer of the developing FVB/N mouse retina.
abstract: The FVB/N mouse is a model of retinitis pigmentosa which shows a rapid loss of photoreceptors during early postnatal (P) life. We investigated the cellular localization of glycine transporter 1 (GlyT-1) in the developing FVB/N mouse retina. In control retinas, the developmental pattern of GlyT-1-immunoreactive amacrine cells was well in accordance with a previous report. However, in the FVB/N mouse retina, some GlyT-1-labeled amacrine cells sent their processes into the outer plexiform layer (OPL) from P14 onward. From P21 onward, GlyT-1-labeled cells were visible in the OPL. These cells were further characterized by double-label immunofluorescence experiments with an antiserum against disabled 1 (Dab-1), and showed Dab-1 immunoreactivity, indicating that these cells are putative AII amacrine cells. These results clearly demonstrate that AII amacrine cells have the potential capacity to respond to photoreceptor degeneration by migrating or sprouting their processes into the OPL in the developing FVB/N mouse retina.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, 137-701 Seoul, Korea.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Lim
authors.forename: Su-Ja
authors.initials: SJ
authors.lastname: Oh
authors.forename: Jin-Woong
authors.initials: JW
authors.lastname: Chung
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Rickman
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Moon
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
mesh_terms: D025042:Amacrine Cells; D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002828:Choristoma / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D019084:Fluorescent Antibody Technique, Indirect; D050487:Glycine Plasma Membrane Transport Proteins; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D012160:Retina / Q000254:growth & development / Q000473:pathology*; D012164:Retinal Diseases / Q000378:metabolism / Q000473:pathology*; D012174:Retinitis Pigmentosa / Q000378:metabolism / Q000473:pathology*
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D050487:Glycine Plasma Membrane Transport Proteins; C493267:Slc6a9 protein, mouse