Digital chemospectrophotographic identification of intracellular hyperlipidemia in diabetic endometrial epithelial cells: structural and metabolic basis of organoatrophy.
OBJECTIVE
Digital chemospectrophotographic (DCSP) microscopic analysis and evaluation methodology applicable for enhanced cytopathological analysis of diabetes-induced, cytohyperlipidemia-associated cellular involution in endometrial epithelial and stromal tissues that promotes reproductive dysfunction and organoatrophy.


METHODS
Combined light microscopy (LM), transmission electron microscopy (TEM) and described DCSP evaluation of endometrial samples collected from control (+/?) and genetically diabetic (db/db) C57BL/KsJ, hyperglycemic-hyperinsulinemic (type II) mice, designed to enhance the intracellular localization of chemically specified triglyceride and free fatty acid depositions, on progressive reproductive tract atrophy and cellular involution indices.


RESULTS
Compared to both the LM and TEM analysis of cytopathological changes associated with diabetes-induced endometrial involution and reproductive dysfunction, the application of DCSP provided enhanced pathovisual analysis of chemical-specific metabolic alterations and cytoplasmic structural changes which accompany cytohyperlipidemia-induced endometrial epithelial cell apoptosis and reproductive tract atrophy.


CONCLUSIONS
DCSP analysis provides an enhanced analytical method for the evaluation of cytoplasmic changes associated with the expression of genomic-, endocrine- or metabolic-based disease states by providing intercytoplasmic specific chemical or metabolic substrate alterations to be identified from conventional pathocellular preparations without requiring the use of exogenous ligand binding or fluorescent methodologies, allowing for a more complete metabolic and cellular evaluation of cytoplasmic indices associated with organoatrophy.
Garris
Bryan L
BL
Division of Cell Biology and Biophysics, Schools of Medicine and Biological Sciences, University of Missouri-Kansas City, Kansas City, Mo. 64110, USA. garrisd@umkc.edu
Garris
David R
DR
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004717:Endometrium / Q000473:pathology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006949:Hyperlipidemias / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D002479:Inclusion Bodies / Q000473:pathology / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D013053:Spectrophotometry