title: The transcriptome of adult female Anopheles darlingi salivary glands.
abstract: Anopheles (Nyssorhynchus) darlingi is an important malaria vector in South and Central America; however, little is known about molecular aspects of its biology. Genomic and proteomic analyses were performed on the salivary gland products of Anopheles darlingi. A total of 593 randomly selected, salivary gland-derived cDNAs were sequenced and assembled based on their similarities into 288 clusters. The putative translated proteins were classified into three categories: (S) secretory products, (H) housekeeping products and (U) products with unknown cell location and function. Ninety-three clusters encode putative secreted proteins and several of them, such as an anophelin, a thrombin inhibitor, apyrases and several new members of the D7 protein family, were identified as molecules involved in haematophagy. Sugar-feeding related enzymes (alpha-glucosidases and alpha-amylase) also were found among the secreted salivary products. Ninety-nine clusters encode housekeeping proteins associated with energy metabolism, protein synthesis, signal transduction and other cellular functions. Ninety-seven clusters encode proteins with no similarity with known proteins. Comparison of the sequence divergence of the S and H categories of proteins of An. darlingi and An. gambiae revealed that the salivary proteins are less conserved than the housekeeping proteins, and therefore are changing at a faster evolutionary rate. Tabular and supplementary material containing the cDNA sequences and annotations are available at http://www.ncbi.nlm.nih.gov/projects/Mosquito/A_darlingi_sialome/
authors.affiliation: University of California, Irvine, Department of Molecular Biology and Biochemistry, Irvine, CA 92697-3900, USA.
authors.forename: E
authors.initials: E
authors.lastname: Calvo
authors.forename: J
authors.initials: J
authors.lastname: Andersen
authors.forename: I M
authors.initials: IM
authors.lastname: Francischetti
authors.forename: M
authors.initials: M
authors.lastname: deL  Capurro
authors.forename: A G
authors.initials: AG
authors.lastname: deBianchi
authors.forename: A A
authors.initials: AA
authors.lastname: James
authors.forename: J M C
authors.initials: JM
authors.lastname: Ribeiro
authors.forename: O
authors.initials: O
authors.lastname: Marinotti
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001938:Brazil; D018076:DNA, Complementary / Q000145:classification*; D030541:Databases, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D012463:Saliva / Q000737:chemistry*; D012469:Salivary Glands / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary