Effects of lipid-esterified conjugated linoleic acid isomers on platelet function: evidence for stimulation of platelet phospholipase activity.
The effects of four conjugated linoleic acid (CLA) isomers on in vitro collagen-induced human platelet aggregation and thromboxane (TXB(2), the inactive metabolite of the proaggregatory TXA(2)) production were examined. As the free fatty acid (FFA), 9t, 11t-CLA was the most effective inhibitor of these two processes (I(50)s of 2.2 and 4 microM, respectively) and the 9c, 11c-CLA was the least effective (I(50)s of 8.3 and 37 microM) of the isomers tested. When platelets were preesterified with either 25 microM 9t, 11t-CLA or 9c, 11c-CLA, CLA incorporation in total platelet lipids increased from 0.24% to 0.31% and 0.38%, and most of this increase was found to be in the phosphatidyl choline and phosphatidyl ethanolamine subclasses. The decrease in arachidonic acid (AA) content in total fatty acids or phospholipids was an order of magnitude greater. Furthermore, no significant differences between platelets prelabeled with either 9t, 11t- or 9c, 11c-CLA in the inhibition of collagen-induced aggregation and TXB(2) formation were observed. However, platelets prelabeled with 9c, 11c-CLA stimulated basal TXB(2) production (4-fold) which was not observed with platelets pretreated with either 9t, 11t-CLA, linoleic acid or stearic acid. This enhancement was associated with a 2.4-5-fold increase in the release of endogenous AA. Our results suggest that the presence of a conjugated cis, cis double bond appears to change the lipid environment sufficiently to stimulate the basal platelet phospholipase activity, which in turn increases the formation of TXB(2).
Al-Madaney
May M
MM
Department of Biochemistry and Molecular Biology, The George Washington University, Washington, DC 20037, USA.
Kramer
John K G
JK
Deng
Zeyuan
Z
Vanderhoek
Jack Y
JY
D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007536:Isomerism; D008041:Linoleic Acids / Q000494:pharmacology; D044243:Linoleic Acids, Conjugated / Q000737:chemistry / Q000494:pharmacology*; D010740:Phospholipases / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000378:metabolism
C094849:10,12-octadecadienoic acid; D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D010975:Platelet Aggregation Inhibitors; C046938:9,11-linoleic acid; D016718:Arachidonic Acid; D013929:Thromboxane B2; D003094:Collagen; D010740:Phospholipases