Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228.
Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene (katL) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli.
Abriouel
Hikmate
H
Institute of Biotechnology and Molecular Biology, Federal Research Centre for Nutrition, D-76131 Karlsruhe, Germany.
Herrmann
Anette
A
Stärke
Joachim
J
Yousif
Nuha M K
NM
Wijaya
Agus
A
Tauscher
Bernhard
B
Holzapfel
Wilhelm
W
Franz
Charles M A P
CM
D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D016983:Enterococcus / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006427:Hemin / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics; D008461:Meat Products / Q000382:microbiology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D006427:Hemin; D002374:Catalase