Pathological change of articular cartilage in the mandibular head treated with immunosuppressant FK 506.
While several reports have documented immunosuppressant-induced osteoporosis, the exact mechanism of the pathological change of the joint remains to be clarified. In the present study, we have demonstrated the pathological change of the articular cartilage in the mandibular head of five Sprague-Dawley rats administered with the immunosuppressant FK 506 for 28 days. Three-dimensional micro-computed tomography of the mandibular heads in treated rats showed a significant decrease in trabecular bone volume compared to control rats. Histological observation revealed atrophic change of the articular cartilage. Immunohistological observation using anti-proliferative cell nuclear antibody (PCNA), type I, II, and type X collagen antibodies showed significantly decreased proliferation and differentiation of chondrocytes in the articular cartilage compared with the control group (p<0.05). Tartrate-resistant acid phosphatase (TRAP) staining revealed no significant difference in the numbers of osteoclasts at the chondro-osseous junction. Thus, FK 506 administration inhibited chondrogenic cell proliferation and differentiation and might cause osteoporotic change of subcartilage trabecular bone that subsequently forms in the mandibular head.
Ueno
T
T
Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata, Okayama, Japan. ueno@md.okayama-u.ac.jp
Kagawa
T
T
Kanou
M
M
Fujii
T
T
Fukunaga
J
J
Mizukawa
N
N
Sugahara
T
T
Yamamoto
T
T
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects* / Q000473:pathology*; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016559:Tacrolimus / Q000494:pharmacology*; D014057:Tomography, X-Ray Computed
D007166:Immunosuppressive Agents; D016559:Tacrolimus