[The study of pollutant's effects on macrophages and its significance in biological monitoring].
This study aimed to investigate the effects of environmental pollutant sulfite and inorganic mercury on the peritoneal macrophages (PM phi) of mice and further explore the significance of macrophage as a biomarker in biological monitoring. PM phi were taken from the peritoneal cavity of mice and cultivated with Na2SO3 and HgCl2 respectively in vitro, and then morphologic changes were detected under light microscope and electron microscope. Also the function of PM phi was detected in yielding nitric oxide (NO), reducing MTT and phagocytizing. After affected by the pollutants, marked morphologic changes of PM phi were observed, and NO reducing, cell vigor and phagocytizing of PM phi were obviously inhibited (p < 0.05 or p < 0.01). And some PM phi were necrotic caused by HgCl2 at high concentration(10(-4) mol/L). The results suggested that PM phi of mice was damaged distinctly by using Na2SO3 and HgCl2 which showed sulfite and inorganic mercury directly impacted on non-special defensive function of macrophages. Meanwhile the experiment indicated that macrophage could be used as an effective biomarker in biological monitoring of environmental contamination.
Guan
Qiong Feng
QF
Institute of Cell Biology, Zhejiang University, Hangzhou 310031.
Li
Ji Cheng
JC
D000818:Animals; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D013447:Sulfites / Q000633:toxicity*
D004785:Environmental Pollutants; D013447:Sulfites; D009569:Nitric Oxide; D008627:Mercuric Chloride; C025026:sodium sulfite