title: Quantitation of 5HT3 receptors in forebrain of serotonin transporter deficient mice.
abstract: Mice deficient in the serotonin transporter (5HTT) display highly elevated extracellular 5HT levels. 5HT exerts ist effects via at least fourteen different cloned 5HT receptors located pre- and postsynaptically. In contrast to the other 5HT receptors, the 5HT3 receptor is a ionotropic receptor with ligand-gated cation channel function. Since G-protein-coupled 5HT receptors show extensive adaptive changes in 5HTT-deficient mice, we investigated whether 5HT3 receptors are also altered in these mice. Using quantitative autoradiography, we found that 5HT3 receptors are upregulated in frontal cortex (+46%), parietal cortex (+42%), and in stratum oriens of the CA3 region of the hippocampus (+18%) of 5HTT knockout mice. Changes in 5HT3 receptor mRNA expression, as determined by quantitative in situ hybridisation, were less pronounced. The adaptive changes of 5HT3 receptor expression constitute a part of the complex regulatory pattern of 5HT receptors in 5HTT knockout mice.
authors.affiliation: Department of Psychiatry and Psychotherapy, University of Wuerzburg, Germany. rainald.moessner@mail.uni-wuerzburg.de
authors.forename: R
authors.initials: R
authors.lastname: Mössner
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
authors.forename: T
authors.initials: T
authors.lastname: Hennig
authors.forename: J
authors.initials: J
authors.lastname: Benninghoff
authors.forename: M
authors.initials: M
authors.lastname: Gerlach
authors.forename: P
authors.initials: P
authors.lastname: Riederer
authors.forename: J
authors.initials: J
authors.lastname: Deckert
authors.forename: K P
authors.initials: KP
authors.lastname: Lesch
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins; D016548:Prosencephalon / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044406:Receptors, Serotonin, 5-HT3 / Q000096:biosynthesis* / Q000235:genetics; D050486:Serotonin Plasma Membrane Transport Proteins
chemical_list: D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D044406:Receptors, Serotonin, 5-HT3; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse