Administration of bone marrow cells into surgically induced fibrocollagenous tunnels induces angiogenesis in ischemic rat hindlimb model.
We established a comparative model of angiogenic induction in previously formed fibrocollagenous tunnels in rat inner thigh muscles. A unilateral hindlimb chronic ischemia model was performed in male Sprague-Dawley rats. A device was then inserted in the central portion of the inner thigh muscles. Vascularity in the ischemic limb was determined by means of an angiographic score, capillary/fiber ratio, and endothelial proliferation by histochemistry and immunohistochemistry. Autologous transplant of bone marrow, vascular endothelial growth factor (VEGF), or collagen-polyvinylpyrrolidone plus heparin induced significant vascularization of the ischemic hindlimb when compared to saline solution. However, the bone marrow group presented a higher angiographic score than the other two. No differences among groups were observed in capillary/fiber ratio or proliferation, except for the VEGF group, where capillary proliferating cells were significantly higher than in controls. Based on these results, bone marrow-derived progenitor cells may constitute a safe and viable alternative for the induction of therapeutic angiogenesis.
Padilla
Luis
L
Department of Microsurgery and Experimental Surgery, Centro Médico Nacional 20 de Noviembre, I.S.S.SF.T.E., Mexico City, Mexico. lpadilla@issste.gob.mx
Krötzsch
Edgar
E
Schalch
Paul
P
Figueroa
Siegfried
S
Miranda
Adriana
A
Rojas
Enrique
E
Esperante
Sandro
S
Villegas
Fernando
F
de la Garza
Anabel S
AS
Di Silvio
Mauricio
M
D000792:Angiography; D000818:Animals; D001707:Biopsy, Needle; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D006085:Graft Survival; D006614:Hindlimb / Q000098:blood supply; D007150:Immunohistochemistry; D007511:Ischemia / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D008297:Male; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000648:ultrastructure; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011336:Probability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014182:Transplantation, Autologous