title: Evaluation of fluorescence spectroscopy methods for mapping melted regions of DNA along the transcription pathway.
authors.affiliation: Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003-9336, USA.
authors.forename: Craig T
authors.initials: CT
authors.lastname: Martin
authors.forename: Andrea
authors.initials: A
authors.lastname: Ujvári
authors.forename: Chihua
authors.initials: C
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D005456:Fluorescent Dyes; D008954:Models, Biological; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012313:RNA / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014158:Transcription, Genetic; D014764:Viral Proteins
chemical_list: D005456:Fluorescent Dyes; D014764:Viral Proteins; D012313:RNA; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases