title: [Calprotectin and total protein in gingival crevicular fluid during experimental gingivitis].
abstract: OBJECTIVE
  To measure GCF calprotectin and total protein for evaluating whether the calprotectin could be a sensitive marker for the initial inflammation of gingiva.
  
  
  METHODS
  Eleven young male subjects with healthy gingiva, who had no systemic diseases, were selected for this study. GCF samples (4 teeth /person) were collected with strips of filter paper at baseline (0 day), on the 7 th, 14 th, 21 st days (without oral hygiene), and 28 th day (7 days after reestablishing oral hygiene) during experimental gingivitis. The amount of calprotectin was measured by ELISA. The amount of total protein was assessed with protein-dye binding assay.
  
  
  RESULTS
  The amount of calprotectin increased during the experimental gingivitis, and reached the highest level on the 21 st day. After oral hygiene was reestablished it reduced to the level of baseline. The amount of total protein had the same tendency as calprotectin.
  
  
  CONCLUSIONS
  The amount of calprotectin and the total protein in GCF can reflect the initial inflammation of gingiva.
authors.affiliation: Department of Periodontology,Peking University Stomatological Hospital, Beijing 100081, China. zhaoyb2000@sina.com
authors.forename: Yibing
authors.initials: Y
authors.lastname: Zhao
authors.forename: Huanxin
authors.initials: H
authors.lastname: Meng
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis*; D008297:Male; D011506:Proteins / Q000032:analysis*
chemical_list: D039841:Leukocyte L1 Antigen Complex; D011506:Proteins