title: Recovery of natural fertility after grafting of cryopreserved germinative tissue in ewes subjected to radiotherapy.
abstract: OBJECTIVE
  To recover natural fertility of ewes that were subjected to ovarian failure induced by radiotherapy with an autologous orthotopic graft of cryopreserved germinative tissue.
  
  
  METHODS
  Experimental surgery study.
  
  
  METHODS
  University hospital unit.
  
  
  METHODS
  Adult ewes.
  
  
  METHODS
  Four ewes were submitted to right oophorectomy and posterior dissecting and freezing of the germinative tissue. Afterward, they were administered radiotherapy to induce infertility on the remaining left ovary. Later, two of the ewes had the thawed fragments of the right ovary injected inside the cortex of the irradiated left ovary in a "sowing" procedure that eliminated the need for sutures.
  
  
  METHODS
  Recovery of fertility in ewes after transplantation of germinative tissue into the ovary destroyed by radiotherapy.
  
  
  RESULTS
  The ewes were housed with fertile rams. Six months following the grafting, the rams impregnated the transplanted ewes. More than 2 years after radiotherapy, the nongrafted (control) ewes have not become pregnant.
  
  
  CONCLUSIONS
  Intracortical grafting of the germinative tissue circumvents the obstacle of vascular anastomosis with autologous ovarian implants. Patients could benefit from the subcortical grafting of germinative tissue in one of the ovaries, recovering fertility after radiotherapy treatment for malignancy.
authors.affiliation: Materbaby-Reprodução Humana e Genética, Maringá, Brazil. almodin@irapida.com.br
authors.forename: Carlos Gilberto
authors.initials: CG
authors.lastname: Almodin
authors.forename: Vania Cibeli
authors.initials: VC
authors.lastname: Minguetti-Câmara
authors.forename: Hugo
authors.initials: H
authors.lastname: Meister
authors.forename: Alvaro Pigatto
authors.initials: AP
authors.lastname: Ceschin
authors.forename: Edgar
authors.initials: E
authors.lastname: Kriger
authors.forename: José Octávio Haggi Rodrigues
authors.initials: JO
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy*; D006080:Ovarian Follicle / Q000502:physiology / Q000637:transplantation*; D010052:Ovariectomy; D010053:Ovary / Q000473:pathology / Q000502:physiology / Q000528:radiation effects*; D011247:Pregnancy; D011878:Radiotherapy / Q000009:adverse effects*; D012756:Sheep; D014184:Transplantation, Homologous