title: Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body.
abstract: Photoluminescent semiconductor quantum dots (QDs) are novel nanometer-size probes that have found bioimaging. Here we imaged a cell line of mouse lymphocytes. QDs were actively taken into the target cells by endocytotic pathways. The fluorescence of QDs held in the endosomes could be studied for more than a week and remained stable luminescence against cell activation induced by concanavalin A, phytohemagglutinin, phorbol myristate acetate, and calcium ionophore A23187. These results suggested that QD-labeling was stable and did not affect either cell activation or cell function. When QD-labeled cells were intravenously injected into mouse, they remained in the peripheral blood in a concentration of approximately 10% up to 5 days after injection using both fluorescence microscopy and flow cytometry. In addition, approximately 20% of QDs were detected in the kidneys, liver, lung, and spleen and could still be observed 7 days after injection. These results suggested that fluorescent probes of QDs might be useful as bioimaging tools for tracing target cells over the period of a week in vivo.
authors.affiliation: Department of Medical Ecology and Informatics, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hoshino
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hanaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D045744:Cell Line, Tumor; D017690:Cell Transplantation / Q000379:methods; D011992:Endosomes / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009928:Organ Specificity; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D014408:Biomarkers, Tumor