Expression of claudin-7 and -8 along the mouse nephron.
Claudins are integral membrane proteins of the tight junction that determine the magnitude and selectivity of paracellular permeability in epithelial tissues. The mammalian renal tubule exhibits considerable heterogeneity in the permeability properties of its different segments. To determine the nephron segment localization of claudin-7 and -8, immunofluorescence staining of mouse kidney sections was performed using isoform-specific antibodies. Claudin-8 was found to be expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons. This pattern of expression is consistent with the putative role of claudin-8 as a paracellular cation barrier. By contrast, claudin-7 was found in the same nephron segments as claudin-8, but it was expressed primarily at the basolateral membrane.
Li
Wing Y
WY
Univ. of Southern California Keck School of Medicine, Div. of Nephrology, 2025 Zonal Ave, RMR 406, Los Angeles, CA 90033, USA.
Huey
Catherine L
CL
Yu
Alan S L
AS
D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D057167:Claudins; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008138:Loop of Henle / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D009399:Nephrons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D019108:Tight Junctions / Q000378:metabolism
D057167:Claudins; D008565:Membrane Proteins; D000450:Aldosterone; C502011:claudin 8