[Application of ISSR technology in genetic diversity detection of jute].
The genetic diversity among 27 accessions of Corchorus, including 10 Jute species, was investigated with ISSR technique. 283 DNA bands were amplified with 25 ISSR primers, among which, 263 (92.85%) were polymorphic, with 10.48 bands per primer in average. A further systemic cluster analysis indicated that the accessions could be clustered into three groups, and the group II (including two cultispecies and their close wild species) was obviously genetically different from the groups I and III (including eight wild species). Moreover, 16 accessions in group II presented a higher intraspecific genetic resemblance, while 11 accessions among groups I & III showed an abundant interspecific genetic diversity. After synthesized the relevant findings of morphology and DNA classification, it's found that C. urticifolius could be one of the original wild species, C. tilaculariszic was a variation of C. tilaculari, and Tian Jute could be an untitled wild species.
Qi
Jianmin
J
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Qijm863@sina.com
Zhou
Dongxin
D
Wu
Weiren
W
Lin
Lihui
L
Wu
Jianmei
J
Fang
Pingping
P
D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D014644:Genetic Variation; D010944:Plants / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers