[Functional reconstruction with tissue engineered myoblast in facial muscle of rat].
OBJECTIVE
The purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.


METHODS
Using purified, subcultured myoblast of neonatal rats and type I collagen gels as extracellular matrix (ECM) and scaffold, tissue engineered muscle was transplanted in the face of syngeneic nutured rats.


RESULTS
Tissue engineered myoblast generated and differentiated in vitro were observed with microscope. Myoblast fused each other and formed myofibers in the face of rats, nerve fibers and vessels regeneration could also be found in some samples. Postoperative electromiographs showed the myofibers were active when stimulating the nerve trunk that innervates the engineered facial muscle.


CONCLUSIONS
Tissue engineered method is hopeful to be used as a new technique to reconstruct defects of facial muscle.
Huang
Guilin
G
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Li
Longjiang
L
Wen
Yuming
Y
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D005152:Facial Muscles / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D005260:Female; D005782:Gels; D008297:Male; D032446:Myoblasts / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures; D023822:Tissue Engineering
D024042:Collagen Type I; D005782:Gels