Binding of imidazole-derived nucleosides to a CG base pair.
Novel imidazole nucleosides with substituents of different flexibility were studied for their binding to a CG Watson-Crick base pair by (1)H NMR spectroscopy in an aprotic solvent. Thermodynamic data as determined by titration experiments at different temperatures reveal the influence of the substituent on the enthalpy and entropy of complex formation and thus on the strength of binding.
Purwanto
Maria G M
MG
Institut für Chemie und Biochemie, Ernst-Moritz-Arndt-Universität Greifswald, Soldmannstrasse 16, D-17489 Greifswald, Germany.
Weisz
Klaus
K
D020029:Base Pairing; D007093:Imidazoles / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D013696:Temperature
D007093:Imidazoles; D009705:Nucleosides