Induction of hepatoma cells migration by phosphoglucose isomerase/autocrine motility factor through the upregulation of matrix metalloproteinase-3.
Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. Presently, we demonstrate that exogenous PGI/AMF stimulates the migration of Huh7 and HepG2 hepatoma cells, but not Hep3B cells. Inhibition of PGI/AMF by PGI/AMF specific inhibitor 5-phospho-D-arabinonate markedly repressed the cellular migration. RT-PCR was used to examine the expression profile of matrix metalloproteinases (MMPs). MMP-3 transcripts, protein level, and secreted form were significantly upregulated in PGI/AMF-treated Huh7 and HepG2 cells, but not in Hep3B cells. MMP-3 inhibition abolished the PGI/AMF-induced cell motility. The observations are consistent with a downstream mediation role of MMP-3 in PGI/AMF-stimulated tumor cell metastasis.
Yu
Feng-Ling
FL
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC.
Liao
Ming-Huei
MH
Lee
Jeng-Woei
JW
Shih
Wen-Ling
WL
D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology*; D002465:Cell Movement; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D019278:Matrix Metalloproteinase 3; D005956:Glucose-6-Phosphate Isomerase