title: Ischemic heart disease induces upregulation of endothelin receptor mRNA in human coronary arteries.
abstract: Endothelin has been implicated in the pathogenesis of ischemic heart disease and congestive heart failure. The aims were to quantify endothelin type A (ETA) and type B (ETB) receptor mRNA levels in human coronary arteries from patients with ischemic heart disease, congestive heart failure and controls using real-time polymerase chain reaction (real-time PCR). In addition, the suitability of organ culture as a model mimicking endothelin receptor changes in cardiovascular disease was evaluated by in vitro pharmacology and real-time PCR. Endothelin ETA and ETB receptor mRNA levels were significantly higher in arteries from patients with ischemic heart disease (0.23+/-0.04 and 0.35+/-0.06) as compared to congestive heart failure (0.09+/-0.02 and 0.07+/-0.01) and controls (0.08+/-0.02 and 0.08+/-0.01). After organ culture, the endothelin ETB receptor mRNA levels were elevated, and the sarafotoxin 6c-induced vasoconstriction was more efficacious. Increased endothelin receptor activity may contribute to the increased vascular tone and development of atherosclerotic disease in ischemic heart disease in man.
authors.affiliation: Department of Internal Medicine, Division of Experimental Vascular Research, BMC A13, Lund University Hospital, SE-221 84, Lund, Sweden. angelica.wackenfors@med.lu.se
authors.forename: Angelica
authors.initials: A
authors.lastname: Wackenfors
authors.forename: Malin
authors.initials: M
authors.lastname: Emilson
authors.forename: Richard
authors.initials: R
authors.lastname: Ingemansson
authors.forename: Tibor
authors.initials: T
authors.lastname: Hortobagyi
authors.forename: Delia
authors.initials: D
authors.lastname: Szok
authors.forename: Janos
authors.initials: J
authors.lastname: Tajti
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vecsei
authors.forename: Lars
authors.initials: L
authors.lastname: Edvinsson
authors.forename: Malin
authors.initials: M
authors.lastname: Malmsjö
mesh_terms: D003331:Coronary Vessels / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000819:agonists / Q000096:biosynthesis*; D044022:Receptor, Endothelin A / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D044023:Receptor, Endothelin B / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B