Ribozyme mediated down-regulation of thrombospondin receptor CD36 inhibits the growth of the human osteosarcoma cell line.
Thrombospondin-1 (TSP1) is known to possess tumor suppressor functions. In contradiction, TSP1 enhances the stromal vascularization and growth of certain cancers. A cell adhesion receptor, CD36, has been shown to interact with a ligand TSP1. We studied how CD36 affects the growth of the osteosarcoma cell line (HOS) expressing TSP1. We used the anti-CD36 ribozyme to specifically suppress CD36 gene expression in the HOS. The expression of the CD36 mRNA was significantly suppressed in the ribozyme-introduced cell line (HOS/Rz). The transformant HOS/Rz markedly decreased its growth. The growth of the osteosarcoma cell line HOS may be regulated by autocrine or paracrine loop TSP1 and CD36.
Yamazaki
Hitoshi
H
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Handa
Akiyoshi
A
Nishi
Masatake
M
Tokunaga
Tetsuji
T
Tomisawa
Masashi
M
Hatanaka
Hiroyuki
H
Abe
Yoshiyuki
Y
Kijima
Hiroshi
H
Ueyama
Yoshito
Y
Nakamura
Masato
M
D015703:Antigens, CD / Q000235:genetics; D001483:Base Sequence; D001859:Bone Neoplasms; D018955:CD36 Antigens / Q000235:genetics*; D002455:Cell Division; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012516:Osteosarcoma; D016337:RNA, Catalytic / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
D015703:Antigens, CD; D018955:CD36 Antigens; D016337:RNA, Catalytic; D012333:RNA, Messenger