Generation of EST and microarray resources for functional genomic studies on chicken intestinal health.
Expressed sequenced tags (ESTs) and microarray resources have a great impact on the ability to study host response in mice and humans. Unfortunately, these resources are not yet available for domestic farm animals. The aim of this study was to provide genomic resources to study chicken intestinal health, in particular malabsorption syndrome (MAS), which affects mainly the intestine. Therefore a normalized and subtracted cDNA library containing more than 7000 clones was prepared. Randomly chosen clones were sequenced for control purposes. New ESTs were found and multiple ESTs not identified in the chicken intestine before were observed. The number of non-specific ESTs in this cDNA library was low. Based on this normalized and subtracted library a cDNA microarray was made. In a preliminary hybridization experiment with the microarray, genes were identified to be up- or downregulated in MAS infected chickens. This indicates that the generated resources are valuable tools to investigate chicken intestinal health by whole genome expression analysis approaches.
van Hemert
Saskia
S
Institute for Animal Science and Health, Lelystad, The Netherlands. saskia.vanhemert@wur.nl
Ebbelaar
Bastiaan H
BH
Smits
Mari A
MA
Rebel
Johanna M J
JM
D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000502:physiology; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D007583:Jejunum / Q000378:metabolism / Q000502:physiology*; D008286:Malabsorption Syndromes / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger