title: Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228.
abstract: Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene (katL) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli.
authors.affiliation: Institute of Biotechnology and Molecular Biology, Federal Research Centre for Nutrition, D-76131 Karlsruhe, Germany.
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.forename: Anette
authors.initials: A
authors.lastname: Herrmann
authors.forename: Joachim
authors.initials: J
authors.lastname: Stärke
authors.forename: Nuha M K
authors.initials: NM
authors.lastname: Yousif
authors.forename: Agus
authors.initials: A
authors.lastname: Wijaya
authors.forename: Bernhard
authors.initials: B
authors.lastname: Tauscher
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Holzapfel
authors.forename: Charles M A P
authors.initials: CM
authors.lastname: Franz
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D016983:Enterococcus / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006427:Hemin / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics; D008461:Meat Products / Q000382:microbiology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D006427:Hemin; D002374:Catalase