Fourier transform infrared spectroscopy as a new tool for characterization of mollicutes.
Fourier transform infrared (FT-IR) spectroscopy is a convenient physico-chemical technique to investigate various cell materials. Bacteria of class Mollicutes, identified by conventional methods, as Mycoplasma, Acholeplasma and Ureaplasma genera were characterized using this method. A data set of 74 independent experiments corresponding to fourteen reference strains of Mollicutes was examined by FT-IR spectroscopy to attempt a spectral characterization based on the biomolecular structures. In addition to the separation of Mollicutes within the lipidic region into five main clusters corresponding to the three phylogenetic groups tested, FT-IR spectroscopy allowed a fine discrimination between strains belonging to the same species by using selective spectral windows, particularly in the 1200-900 cm(-1) saccharide range. The results obtained by FT-IR were in good agreement with both taxonomic and phylogenetic classifications of tested strains. Thus, this technique appears to be a useful tool and an accurate mean for a rapid characterization of Mollicutes observed in humans.
Melin
Anne-Marie
AM
INSERM U577-Groupe de Chimie Bio-Organique, France.
Allery
Annie
A
Perromat
Annie
A
Bébéar
Christiane
C
Déléris
Gérard
G
de Barbeyrac
Bertille
B
D002241:Carbohydrates / Q000737:chemistry; D016000:Cluster Analysis; D010710:Phosphates / Q000737:chemistry; D010802:Phylogeny; D025341:Principal Component Analysis; D011506:Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D008973:Tenericutes / Q000737:chemistry* / Q000145:classification
D002241:Carbohydrates; D010710:Phosphates; D011506:Proteins