Glucocorticoid-induced thymocyte apoptosis in wall lizard Hemidactylus flaviviridis.
The aim of the present study is to demonstrate the phenomenon and mechanism of GC-induced cell death of thymocytes in wall lizard Hemidactylus flaviviridis. Lizard thymocytes were treated in vitro with different concentration of corticosterone (CS) for varying durations and the genomic DNA was analyzed both by agarose gel electrophoresis and flow cytometry. Corticosterone induced DNA fragmentation in a dose-dependent manner, as the DNA laddering was more prominent at 10(-14) M than at lower concentrations. However, CS could activate the programmed cell death of thymocytes only when incubated for 48 h and not at 3, 6, 12, or 24 h. Similar results were obtained following the flow cytometric analysis. The highest apoptotic cell death was noted at 10(-14) M concentration. CS-induced programmed cell death was decreased dramatically when the thymocytes were preincubated with RU 486 (10(-9) ng /ml) for 24 h, suggesting the classical receptor-mediated genomic pathway of glucocorticoid action in inducing apoptosis of thymocytes in wall lizard H. flaviviridis.
Hareramadas
B
B
Comparative Endocrinology laboratory, Department of Zoology, University of Delhi (North Campus), 110 007, India.
Rembhotkar
G W
GW
Rai
U
U
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology / Q000502:physiology; D004247:DNA / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005260:Female; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology* / Q000502:physiology; D008116:Lizards / Q000502:physiology*; D015735:Mifepristone / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology*
D005938:Glucocorticoids; D015735:Mifepristone; D004247:DNA; D003345:Corticosterone