Target-selected mutagenesis of the rat.
The rat is one of the most extensively studied model organisms, and with its genome being sequenced, tools to manipulate gene function in vivo have become increasingly important. We here report proof of principle for target-selected mutagenesis as a reverse genetic or knockout approach for the rat.
Smits
Bart M G
BM
Functional Genomics Group, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Mudde
Josine
J
Plasterk
Ronald H A
RH
Cuppen
Edwin
E
D000818:Animals; D004252:DNA Mutational Analysis; D005038:Ethylnitrosourea; D005298:Fertility / Q000235:genetics; D005640:Follicle Stimulating Hormone / Q000235:genetics; D018095:Germ-Line Mutation; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar
D009153:Mutagens; D005640:Follicle Stimulating Hormone; D005038:Ethylnitrosourea