An automatic method for the determination of carcinogenic 1-nitropyrene in extracts from automobile exhaust particulate matter.
An automatic method for the determination of carcinogenic 1-nitropyrene in extracts from automobile exhaust particulate matter has been developed. Organic matter was extracted from particulate matter and the extract was concentrated. Then the extract was injected into a two-dimensional (2D) high-performance liquid chromatograph (HPLC) with a fluorescence detection system. To improve the sensitivity a large sample volume (300 microl) was applied to the HPLC system using the double on-column focusing technique. To achieve full automation and high selectivity a reduction column packed with platinum black was introduced after the 2D HPLC system using a C18 column as the primary column and a pentabromobenzyl column as the secondary column. This HPLC system could determine 0.01-3,000 ng ml(-1) of 1-nitropyrene in an extract from diesel and gasoline exhaust particulate matter at 40 min intervals.
Murahashi
Tsuyoshi
T
Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, 607-8414 Kyoto, Japan. tmu@bj8.so-net.ne.jp
Tsuruga
Fumiko
F
Sasaki
Sousuke
S
D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D001330:Electronic Data Processing; D011721:Pyrenes / Q000032:analysis*; D012680:Sensitivity and Specificity; D001335:Vehicle Emissions / Q000032:analysis*
D002273:Carcinogens; D011721:Pyrenes; D001335:Vehicle Emissions; C032668:1-nitropyrene