title: Isolation of an ozone-sensitive and jasmonate-semi-insensitive Arabidopsis mutant (oji1).
abstract: A novel ozone-sensitive mutant was isolated from Arabidopsis T-DNA tagging lines. This mutant revealed severe foliar injury and higher ethylene emission than the wild type under ozone exposure. The ozone-induced injury and ethylene emission were suppressed by pretreatment with aminoethoxyvinyl glycine, an inhibitor of ethylene biosynthesis, both in this mutant and wild-type plants. Pretreatment with methyl-jasmonate (MeJA) at 10 micro M, however, suppressed the ozone-induced ethylene emission and foliar injury only in the wild-type plants. This mutant was less sensitive to jasmonate than the wild type, estimated by the MeJA-induced inhibition of root elongation and ozone-induced expression of AtVSP1, a jasmonate-inducible gene. Thus, this mutant was named oji1 (ozone-sensitive and jasmonate-insensitive 1). These results suggest that the ozone sensitivity of oji1 is caused by the increase in ozone-induced emission of ethylene as a result of low sensitivity to jasmonate, which plays defensive roles under stress conditions.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501 Japan.
authors.forename: Machi
authors.initials: M
authors.lastname: Kanna
authors.forename: Masanori
authors.initials: M
authors.lastname: Tamaoki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kubo
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Nakajima
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tamogami
authors.forename: Motohide
authors.initials: M
authors.lastname: Ioki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogawa
authors.forename: Hikaru
authors.initials: H
authors.lastname: Saji
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Aono
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D003517:Cyclopentanes / Q000494:pharmacology*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis; D009154:Mutation; D054883:Oxylipins; D010126:Ozone / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010126:Ozone; C072239:methyl jasmonate; C036216:ethylene; C441479:VSP1 protein, Arabidopsis; D010450:Endopeptidases