Kv1 currents mediate a gradient of principal neuron excitability across the tonotopic axis in the rat lateral superior olive.
Principal neurons of the lateral superior olive (LSO) detect interaural intensity differences by integration of excitatory projections from ipsilateral bushy cells and inhibitory inputs from the medial nucleus of the trapezoid body. The intrinsic membrane currents active around firing threshold will form an important component of this binaural computation. Whole cell patch recording in an in vitro brain slice preparation was employed to study conductances regulating action potential (AP) firing in principal neurons. Current-clamp recordings from different neurons showed two types of firing pattern on depolarization, one group fired only a single initial AP and had low input resistance while the second group fired multiple APs and had a high input resistance. Under voltage-clamp, single-spiking neurons showed significantly higher levels of a dendrotoxin-sensitive, low threshold potassium current (ILT). Block of ILT by dendrotoxin-I allowed single-spiking cells to fire multiple APs and indicated that this current was mediated by Kv1 channels. Both neuronal types were morphologically similar and possessed similar amounts of the hyperpolarization-activated nonspecific cation conductance (Ih). However, single-spiking cells predominated in the lateral limb of the LSO (receiving low frequency sound inputs) while multiple-firing cells dominated the medial limb. This functional gradient was mirrored by a medio-lateral distribution of Kv1.1 immunolabelling. We conclude that Kv1 channels underlie the gradient of LSO principal neuron firing properties. The properties of single-spiking neurons would render them particularly suited to preserving timing information.
Barnes-Davies
Margaret
M
Department of Cell Physiology & Pharmacology, University of Leicester, PO Box 138, Leicester LE1 9HN, UK.
Barker
Matthew C
MC
Osmani
Fatima
F
Forsythe
Ian D
ID
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count / Q000379:methods; D004546:Elapid Venoms / Q000494:pharmacology; D066298:In Vitro Techniques; D051662:Kv1.1 Potassium Channel; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009847:Olivary Nucleus / Q000187:drug effects / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats
D004546:Elapid Venoms; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; C108582:dendrotoxin I; D051662:Kv1.1 Potassium Channel