[Expression of constructed eukaryotic vectors carrying encoding gene of soluble human interleukin-1 receptor in mammalian cells].
OBJECTIVE
This study is to make sure if the constructed pcDNA3 carrying encoding gene of sIL-1R can be expressed in mammalian cells in vitro.


METHODS
COS-1 cells and CHO cells were respectively transfected with recombinant plasmid pcDNA3/sIL-1R by liposome. The protein expression products were detected by ELISA.


RESULTS
The results indicated that the protein expression products could be detected in the cell plasma and the cell culture supermatant. The expression level in experimental groups was much higher than that in control groups(P < 0.05).


CONCLUSIONS
The constructed pcDNA3/sIL-1R can express interest protein in mammalian cells and this establishes the basis for future investigation on gene therapy of periodonititis and other inflammatory diseases.
Xu
Yan
Y
Department of Oral Medicine, College of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Li
Peng
P
Zhang
Yunhui
Y
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005057:Eukaryotic Cells / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D011995:Recombination, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D018076:DNA, Complementary; D017472:Receptors, Interleukin-1; D011993:Recombinant Fusion Proteins