Restricted expression and photic induction of a novel mouse regulatory factor X4 transcript in the suprachiasmatic nucleus.
The regulatory factor X (RFX) family of transcription factors is characterized by a unique and highly conserved 76-amino acid residue DNA-binding domain. Mammals have five RFX genes, but the physiological functions of their products are unknown, with the exception of RFX5. Here a mouse RFX4 transcript was identified that encodes a peptide of 735 amino acids, including the DNA-binding domain. Its expression was localized in the suprachiasmatic nucleus, the central pacemaker site of the circadian clock. Also, light exposure was found to induce its gene expression in a subjective night-specific manner. Polyclonal antibodies were prepared, and an 80-kDa band was detected in the suprachiasmatic nucleus by Western hybridization. A histochemical study showed a localization of the products in the nucleus. This is the first report on mouse RFX4, which contains the RFX DNA-binding motif. Our investigation may provide clues to the physiological function of RFX4.
Araki
Ryoko
R
Transcriptome Research Center, National Institute of Radiological Sciences, Chiba-shi, Chiba 263-8555, Japan.
Takahashi
Hirokazu
H
Fukumura
Ryutaro
R
Sun
Fuyan
F
Umeda
Nanae
N
Sujino
Mitsugu
M
Inouye
Shin-Ichi T
ST
Saito
Toshiyuki
T
Abe
Masumi
M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D019556:COS Cells; D002940:Circadian Rhythm; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008027:Light; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D000071701:Regulatory Factor X Transcription Factors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010455:Peptides; D012333:RNA, Messenger; D000071701:Regulatory Factor X Transcription Factors; C479564:Rfx4 protein, mouse; D014157:Transcription Factors; D012313:RNA; D004247:DNA