title: Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene.
abstract: The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host we used a polyketide synthase-defective mutant strain of Streptomyces fradiae Tü2717 which is not able to produce urdamycin A. Our results indicate that four glycosyltransferases are responsible for the formation of the hexasaccharide side chain of landomycin A.
authors.affiliation: Albert-Ludwigs-University of Freiburg, Pharmaceutical Biology, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: von Mulert
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
authors.forename: Carsten
authors.initials: C
authors.lastname: Hofmann
authors.forename: Almuth
authors.initials: A
authors.lastname: Mayer
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics*; D005810:Multigene Family; D009154:Mutation; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D001426:Bacterial Proteins; D009097:Multienzyme Complexes; C064648:landomycin A; D016695:Glycosyltransferases