[Characteristic of saccharose fermentation by pathogenic Escherichia coli strains--phenotypic and genotypic elements].
The purpose of the study was to characterize fermentation of sucrose by Escherichia coli strains and to answer why some of these strains doesn't utilize this disaccharide. Investigations included 16 E. coli strains. Only 5 of these strains utilized sucrose. Genotypic analysis demonstrated the presence of cscB gene (encoding the sucrase permease which catalyzes transport of sucrose through the plasma membrane of the cell) in 5 strains of E. coli and cscA gene (encoding an enzyme sucrase that catalyzes the utilization of sucrose) in 6 strains of E. coli. These 5 of E. coli strains which possessed a chromosomally encoded sucrose metabolic pathway utilized sucrose with a different time. 3 of them destroyed this disaccharide after 24 h and 2 of them destroyed it after 48 h. Ten of E. coli strains hadn't cscA gene and 11 of them had not cscB genes. The lack of these genes can be the prove that it is not possible for 11 of E. coli strains to synthesize sucrose permease and for 10 of them to synthesize sucrase and it may be the reason of not utilize disaccharide sucrose by these bacteria.
Skwark
Małgorzata
M
Katedra Immunologii i Mikrobiologii Wydziału Biotechnologii i Hodowli Zwierzat Akademii Rolniczej w Szczecinie.
Czyzewska
Izabela
I
D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005285:Fermentation; D005838:Genotype; D026901:Membrane Transport Proteins / Q000235:genetics; D010641:Phenotype; D013393:Sucrase / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*
D026901:Membrane Transport Proteins; D013395:Sucrose; D013393:Sucrase