Structural analysis of sphingophospholipids derived from Sphingobacterium spiritivorum, the type species of genus Sphingobacterium.
The unique feature of the genus Sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. As major cellular lipid components, five kinds of sphingophospholipids were purified from Sphingobacterium spiritivorum ATCC 33861(T), the type species of genus Sphingobacterium. They were identified as ceramide phosphorylethanolamines (CerPE-1 and CerPE-2), ceramide phosphoryl-myo-inositols (CerPI-1 and CerPI-2), and ceramide phosphorylmannose (CerPM-1). The ceramide of CerPE-1, CerPI-1, and CerPM-1 was composed of 15-methylhexadecasphinganine (isoheptadeca sphinganine, iso-C17:0) and 13-methyltetradecanoic acid (isopentadecanoic acid, iso-C15:0), whereas that of CerPE-2 and CerPI-2 was composed of isoheptadeca sphinganine and 2-hydroxy-13-methyltetradecanoic acid (2-hydroxy isopentadecanoic acid, 2-OH iso-C15:0). These sphingophospholipids were also found in cellular lipids of Sphingobacterium multivorum ATCC 33613(T), Sphingobacterium mizutaii ATCC 33299(T), Sphingobacterium faecium IFO 15299(T), Sphingobacterium thalpophilum ATCC 43320(T), and Sphingobacterium antarcticum ATCC 51969(T). To our knowledge, the existence of CerPM-1 is a novel sphingophospholipid through eukaryotic and prokaryotic cells.
Naka
Takashi
T
Department of Host Defense, Osaka City University Graduate School of Medicine, Japan. tnaka@med.osaka-cu.ac.jp
Fujiwara
Nagatoshi
N
Yano
Ikuya
I
Maeda
Shinji
S
Doe
Matsumi
M
Minamino
Miki
M
Ikeda
Norikazu
N
Kato
Yoshiko
Y
Watabe
Kazuhito
K
Kumazawa
Yoshio
Y
Tomiyasu
Ikuko
I
Kobayashi
Kazuo
K
D002518:Ceramides / Q000032:analysis; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D045249:Sphingobacterium / Q000737:chemistry*; D013107:Sphingolipids / Q000737:chemistry* / Q000302:isolation & purification
D002518:Ceramides; D005227:Fatty Acids; D010743:Phospholipids; D013107:Sphingolipids; C047140:isopentadecanoic acid