Analysis of five differentially expressed gene families in fast elongating cotton fiber.
Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0 C20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family --AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer protein may contribute directly to the elongation and development of fiber cells.
Feng
Jian-Xun
JX
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Ji
Sheng-Jian
SJ
Shi
Yong-Hui
YH
Xu
Yu
Y
Wei
Gang
G
Zhu
Yu-Xian
YX
D000595:Amino Acid Sequence; D032561:Cotton Fiber; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D015533:Transcriptional Activation / Q000235:genetics
D010940:Plant Proteins