title: Overexpression of gastrin and c-met protein involved in human gastric carcinomas and intestinal metaplasia.
abstract: Many studies have investigated the expression of c-met and c-erbB2 protein in human gastric adenocarcinomas, but the expression of gastrin protein in human gastric cancer and the relationship between gastrin and c-met are unknown. We have constructed a tissue microarray containing 408 formalin-fixed and paraffin-embedded human tissue blocks, including tissues containing intestinal metaplasia (IM, n=72) and primary tumors (n=232), as well as normal gastric mucosa (n=104) from patients with gastric cancer. Immunohistochemistry (IHC) was used for detecting gastrin, c-met and c-erbB2 proteins. Gastrin was detected in 13.5% (7/52) and c-met in 15.3% (11/72) of IM cases. In gastric carcinomas, 48.4% (103/213) of cases expressed gastrin, 68.8% (148/215) expressed c-met, and 5.5% (11/200) expressed c-erbB2. Gastrin and c-met protein expression were significantly higher in gastric tumor tissue than in IM (P<0.0001). Overexpression of c-erbB2 protein was detected in gastric carcinomas but not in normal gastric mucosa (P<0.05). Expression of gastrin and c-met protein was associated (P<0.01), but no significant difference was found on the changes of gastrin, c-met and c-erbB2 expression in gastric cancer with tumor stage, grade of differentiation or tumor type. These results indicate that gastrin and c-met play a role in the early process during malignant transformation of the gastric mucosa.
authors.affiliation: Beijing Laboratory of Molecular Oncology, Peking University School of Oncology, Beijing, P.R. China.
authors.forename: Zhuobin
authors.initials: Z
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Jiafu
authors.initials: J
authors.lastname: Ji
authors.forename: Guibin
authors.initials: G
authors.lastname: Yang
authors.forename: Fulian
authors.initials: F
authors.lastname: Hu
authors.forename: Jingsheng
authors.initials: J
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.forename: Zhian
authors.initials: Z
authors.lastname: Gao
authors.forename: Ailian
authors.initials: A
authors.lastname: Zhao
authors.forename: Jiyou
authors.initials: J
authors.lastname: Li
authors.forename: Youyong
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D003937:Diagnosis, Differential; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D008679:Metaplasia / Q000473:pathology*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000473:pathology* / Q000601:surgery
chemical_list: D005755:Gastrins; D019859:Proto-Oncogene Proteins c-met