Taurine prevents acrylonitrile-induced oxidative stress in rat brain.
Acrylonitrile (ACN) is a volatile, toxic liquid used as a monomer in the manufacture of synthetic rubber, styrene plastics, acrylic fiber and adhesives. ACN is a potent neurotoxin and a carcinogen, which produces tumors in rats, particularly gliomas of the brain. A role for free radical-mediated lipid peroxidation in the toxicity of ACN has been suggested. We examined the ability of taurine, an antioxidant amino acid, to attenuate ACN-induced alterations in lipid peroxidation, cellular DNA fragmentation, GSH, vitamin C and vitamin E levels in blood and brain of rats. Rats were administered with ACN at a concentration of 100 ppm in drinking water and sacrificed after 14 and 28 days. The level of lipid peroxidation and the enzymatic and non-enzymatic antioxidants were assayed. The obtained data were compared with those obtained from ACN rats co-treated with taurine for 14 and 28 days. It was observed that taurine treatment counteracted the oxidative stress induced by ACN by reducing the levels of peroxidation, and enhancing the activities of enzymatic and non-enzymatic antioxidants.
Mahalakshmi
Karthikeyan
K
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar--608 002, Tamil Nadu, India.
Pushpakiran
Gullapalli
G
Anuradha
Carani V
CV
D000181:Acrylonitrile / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002374:Catalase / Q000097:blood / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004334:Drug Administration Schedule; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D002273:Carcinogens; D017392:Thiobarbituric Acid Reactive Substances; D013654:Taurine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D024502:alpha-Tocopherol; D000181:Acrylonitrile; D001205:Ascorbic Acid