In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines.
The modification of cellular DNA by environmental substances is thought to be a crucial event in chemical induced carcinogenesis. Among the environmental carcinogens, aromatic amines are known for the fact that they can induce several types of cancers through the formation of so-called DNA adducts. We took advantage of the potential of the SELEX method to select for highly specific RNA ligands that recognize specific genotoxic aromatic amines. The aromatic amine 4,4'-methylenedianiline (MDA) was used as a target. Following in vitro selection, we obtained specific MDA-binding RNA molecules based on an affinity chromatography assay. These results open the possibility of using the SELEX technique to generate RNA molecules as diagnostic tools for the detection of DNA damaging compounds and ultimately DNA adducts.
Brockstedt
Ulrike
U
Centre de recherche, Hôpital Sainte-Justine 3175 Côte Sainte-Catherine, Montréal, Que., Canada H3T 1C5.
Uzarowska
Anna
A
Montpetit
Alexandre
A
Pfau
Wolfgang
W
Labuda
Damian
D
D000588:Amines / Q000737:chemistry* / Q000633:toxicity; D001483:Base Sequence; D002273:Carcinogens / Q000737:chemistry* / Q000633:toxicity; D002846:Chromatography, Affinity; D018736:DNA Adducts / Q000737:chemistry / Q000235:genetics; D019020:Directed Molecular Evolution; D066298:In Vitro Techniques; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification*
D000588:Amines; D002273:Carcinogens; D018736:DNA Adducts; D012313:RNA