title: Irish strains of Neisseria meningitidis: characterisation using multilocus sequence typing.
abstract: A total of 56 Neisseria meningitidis strains are analysed using multilocus sequence typing (MLST). Twenty-nine distinct sequence types (STs) were identified, eight of which were new. Four known hypervirulent clones--ST-11 (electrophoretic type [ET]-37) complex, ST-44 complex (lineage 3), ST-32 (ET-5) complex and ST-8 complex (cluster A4)--were identified by MLST in 35 disease-associated and four carrier strains. Two other clones (ST-22 complex and ST-269 complex) were identified in nine disease-associated and one carrier strain. The remaining strains were heterogeneous. Additional sequencing within the FumC gene further distinguished the ET-15 clone within the ST-11 (ET-37) clonal complex. This resolution of isolates into genetic clones by MLST enhances the more traditional techniques of serotyping and serosubtyping. The data obtained established that hyperendemic meningococcal disease in Ireland could be attributed to strains belonging to four major hypervirulent clones, all of which account for elevated levels of disease worldwide. The extra information provided by MLST will be used to study the population structure and epidemiology of N. meningitidis and will allow a comparison of Irish strains with those circulating globally.
authors.affiliation: Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Temple Street, Dublin 1, Ireland. karen.murphy@tsch.ie
authors.forename: K M
authors.initials: KM
authors.lastname: Murphy
authors.forename: K A
authors.initials: KA
authors.lastname: O'Donnell
authors.forename: A B
authors.initials: AB
authors.lastname: Higgins
authors.forename: C
authors.initials: C
authors.lastname: O'Neill
authors.forename: M T
authors.initials: MT
authors.lastname: Cafferkey
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008589:Meningococcal Infections / Q000382:microbiology*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000145:classification*; D017422:Sequence Analysis, DNA / Q000379:methods; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial