A recombination event, induced in ovo, between a low passage infectious bronchitis virus field isolate and a highly embryo adaptedvaccine strain.
The possibility of genomic recombination among different strains of infectious bronchitis virus (IBV) was examined in eve by coinfecting specific pathogen free embryonating chicken eggs with commonly used, embryo-adapted vaccine strains of IBV (Arkansas, Massachusetts, and Connecticut), and a Delaware-072-like field virus isolated from a layer farm in Minnesota. Recombination was observed between th e Massachusetts and the Delaware-072-like strains of the virus. The recombination event was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR) using a combination of specific primers designed to flank a known recombination hot spot of the viral genomic sequence that codes for the S1 subunit of the spike envelope protein. The use of these primers allowed the detection of viruses that have undergone recombination around this hot spot. Cloning and sequencing of the RT-PCR product obtained was performed to confirm these results.
Estevez
Carlos
C
Poultry Diagnostic and Research Center, Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-4875, USA.
Villegas
Pedro
P
El-Attrache
John
J
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000821:virology*; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary; D004625:Embryo, Nonmammalian / Q000821:virology*; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology; D011995:Recombination, Genetic; D019444:Vaccines, DNA; D014765:Viral Vaccines
D019444:Vaccines, DNA; D014765:Viral Vaccines