Generation of a complete set of human telomeric band painting probes by chromosome microdissection.
Chromosomal rearrangements involving telomeric bands have been frequently detected in many malignancies and congenital diseases. To develop a useful tool to study chromosomal rearrangements within the telomeric band effectively and accurately, a whole set of telomeric band painting probes (TBP) has been generated by chromosome microdissection. The intensity and specificity of these TBPs have been tested by fluorescence in situ hybridization and all TBPs showed strong and specific signals to target regions. TBPs of 6q and 17p were successfully used to detect the loss of the terminal band of 6q in a hepatocellular carcinoma cell line and a complex translocation involving the 17p terminal band in a melanoma cell line. Meanwhile, the TBP of 21q was used to detect a de novo translocation, t(12;21), and the breakpoint at 21q was located at 21q22.2. Further application of these TBPs should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements involving telomeric bands.
Hu
Liang
L
Department of Clinical Oncology, The University of Hong Kong, Room 109, School of Chinese Medicine Building, 10 Sassoon Road, Hong Kong, China.
Sham
Jonathan S T
JS
Tjia
Wai Mui
WM
Tan
Yue-qiu
YQ
Lu
Guang-xiu
GX
Guan
Xin-Yuan
XY
D045744:Cell Line, Tumor; D002871:Chromosome Banding / Q000379:methods*; D019457:Chromosome Breakage; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002891:Chromosomes, Human, Pair 21; D002896:Chromosomes, Human, Pair 6; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016615:Telomere / Q000235:genetics*; D014178:Translocation, Genetic
D015342:DNA Probes