A first generation bovine BAC-based physical map.
A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105,984 clones) and a part of the CHORI-240 BAC library (26,500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100,923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.
Schibler
Laurent
L
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas, France. schibler@jouy.inra.fr
Roig
Anne
A
Mahé
Marie-Françoise
MF
Save
Jean-Claude
JC
Gautier
Mathieu
M
Taourit
Sead
S
Boichard
Didier
D
Eggen
André
A
Cribiu
Edmond P
EP
D000818:Animals; D002417:Cattle / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D016172:DNA Fingerprinting; D005819:Genetic Markers; D016678:Genome; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D005819:Genetic Markers