Anticlastogenicity of chlorophyllin in the different cell cycle phases in cultured mammalian cells.
Chlorophyllin (Chln), a sodium-copper salt derivative of chlorophyll, like chlorophyll-a and -b found in green plants, has been studied for its protective action against the carcinogenic effects of various physical and chemical agents and in relation to the mutagenic and clastogenic activities of genotoxic agents. The aim of the present study was to evaluate chlorophyllin in different phases of the cell cycle for clastogenicity and anticlastogenicity, the latter in reversing DNA damage induced by ethyl methane sulfonate (EMS). The test for chromosomal aberrations was performed in cultured mammalian cells (CHO-K1). The three Chln concentrations tested (6.25, 12.5 and 25 microg/ml) were not clastogenic and damage induced by EMS (1240 microg/ml) was reduced in cells treated with Chln as well during S (25-48%) and G2/S (70-80%). The results demonstrate a greater protective effectiveness of Chln against EMS during G2/S.
Negraes
P D
PD
Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, Londrina PR 86051-990, Brazil.
Jordão
B Q
BQ
Vicentini
V E P
VE
Mantovani
M S
MS
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002735:Chlorophyllides / Q000494:pharmacology*; D006224:Cricetinae; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug
D016587:Antimutagenic Agents; D002735:Chlorophyllides; C007020:chlorophyllin