Development of cultivated mucosal epithelial sheet transplantation for ocular surface reconstruction.
Regenerative medicine via tissue engineering is a newly developed medical field based on the use of somatic stem cells to generate biological substitutes and improve tissue functions. The achievement of these objectives depends on two important factors: stem cells, which are high in proliferability and differentiability, and the substrates that support them. In order to reconstruct the ocular surface in patients with severe ocular surface diseases, we decided to investigate the feasibility of human amniotic membrane as an epithelial carrier, and found that denuded amniotic membrane was the most appropriate substrate for this purpose. To develop the surgical treatment using denuded amniotic membrane as a carrier, we established the tissue engineering system for making transplantable epithelial sheets of either corneal or oral mucosal epithelial cells for ocular surface reconstruction.
Kinoshita
Shigeru
S
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. skinoshi@ophth.kpu-m.ac.jp
Nakamura
Takahiro
T
D000650:Amnion / Q000166:cytology; D000818:Animals; D002478:Cells, Cultured; D003315:Cornea / Q000601:surgery*; D065306:Corneal Injuries; D004847:Epithelial Cells / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000166:cytology / Q000637:transplantation*; D005240:Feasibility Studies; D005612:Freeze Drying; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D013508:Ophthalmologic Surgical Procedures / Q000379:methods; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*