Identification of amino acid residues crucial for chemokine receptor dimerization.
Chemokines coordinate leukocyte trafficking by promoting oligomerization and signaling by G protein-coupled receptors; however, it is not known which amino acid residues of the receptors participate in this process. Bioinformatic analysis predicted that Ile52 in transmembrane region-1 (TM1) and Val150 in TM4 of the chemokine receptor CCR5 are key residues in the interaction surface between CCR5 molecules. Mutation of these residues generated nonfunctional receptors that could not dimerize or trigger signaling. In vitro and in vivo studies in human cell lines and primary T cells showed that synthetic peptides containing these residues blocked responses induced by the CCR5 ligand CCL5. Fluorescence resonance energy transfer showed the presence of preformed, ligand-stabilized chemokine receptor oligomers. This is the first description of the residues involved in chemokine receptor dimerization, and indicates a potential target for the modification of chemokine responses.
Hernanz-Falcón
Patricia
P
Department of Immunology and Oncology, National Center of Biotechnology, Campus Universitario de Cantoblanco, E-28049 Madrid, Spain.
Rodríguez-Frade
José Miguel
JM
Serrano
Antonio
A
Juan
David
D
del Sol
Antonio
A
Soriano
Silvia F
SF
Roncal
Fernando
F
Gómez
Lucio
L
Valencia
Alfonso
A
Martínez-A
Carlos
C
Mellado
Mario
M
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D020836:Protein Structure, Quaternary; D019713:Receptors, CCR5 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D000596:Amino Acids; D008024:Ligands; D009842:Oligopeptides; D019713:Receptors, CCR5; D011994:Recombinant Proteins