title: Flavivirus isolations from mosquitoes collected from western Cape York Peninsula, Australia, 1999-2000.
abstract: After the 1st appearance of Japanese encephalitis virus (JE) on mainland Australia in 1998, a study was undertaken to investigate whether JE had become established in enzootic transmission cycles on western Cape York Peninsula. Adult mosquitoes were collected during the late wet season from Kowanyama and Pormpuraaw in April 1999, and Pormpuraaw and Barr's Yard in April 2000. Despite processing 269,270 mosquitoes for virus isolation, no isolates of JE were obtained. However, other flaviviruses comprising Murray Valley encephalitis virus, Kunjin virus, Alfuy virus, and Kokobera virus (KOK) were isolated. Isolates of the alphaviruses Ross River virus, Barmah Forest virus (BF), and Sindbis virus (SIN) also were obtained. The majority (88%) of isolates were from members of the Culex sitiens subgroup. Single isolates of KOK, BF, and SIN were obtained from Ochlerotatus vigilax, Oc. normanensis, and Anopheles bancroftii, respectively. The isolations of flaviviruses during the late wet season indicate that conditions were suitable for flavivirus activity in the area. No evidence was found to suggest that JE has become established in enzootic transmission cycles on western Cape York, although study sites and field trips were limited.
authors.affiliation: Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Johansen
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Nisbet
authors.forename: Paul
authors.initials: P
authors.lastname: Zborowski
authors.forename: Andrew F
authors.initials: AF
authors.lastname: van den Hurk
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ritchie
authors.forename: John S
authors.initials: JS
authors.lastname: Mackenzie
mesh_terms: D000524:Alphavirus / Q000302:isolation & purification; D000818:Animals; D000852:Anopheles / Q000821:virology; D003465:Culex / Q000821:virology; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D018068:Encephalitis Virus, Murray Valley / Q000302:isolation & purification; D044969:Ochlerotatus / Q000821:virology; D011793:Queensland; D012398:Ross River virus / Q000302:isolation & purification; D012845:Sindbis Virus / Q000302:isolation & purification; D014902:West Nile virus / Q000302:isolation & purification