title: Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata.
abstract: Neuropeptides of the capa-gene are typical of the abdominal neurosecretory system of insects. In this study, we investigated these peptides in two widely distributed and large pest flies, namely Musca domestica and Neobellieria bullata. Using a combination of MALDI-TOF and ESI-QTOF mass spectrometry, periviscerokinins and a pyrokinin were analyzed from single perisympathetic organ preparations. The species-specific peptide sequences differ remarkably between the related dipteran species. These differences could make it possible to develop peptide-analogs with group- or species-specific efficacy.
authors.affiliation: Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, USA. b6prre@pan.zoo.uni-jena.de
authors.forename: Reinhard
authors.initials: R
authors.lastname: Predel
authors.forename: William K
authors.initials: WK
authors.lastname: Russell
authors.forename: Shane E
authors.initials: SE
authors.lastname: Tichy
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Nachman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004175:Diptera / Q000737:chemistry*; D029721:Drosophila Proteins / Q000737:chemistry*; D006793:Houseflies / Q000737:chemistry*; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry*; D009490:Neurosecretory Systems / Q000737:chemistry; D016415:Sequence Alignment; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D009479:Neuropeptides; C455043:capa protein, Drosophila