A naturally occurring -263G/C variant of the human AA-NAT gene and overnight melatonin production.
Several lines of evidence show that the daily amount of melatonin produced differs greatly between individuals. Any polymorphism in the gene of arylalkylamine N-acetyltransferase (AA-NAT), a critical enzyme involved in melatonin biosynthesis, may contribute to the variability of melatonin production. The present study investigated the possible association between overnight melatonin excretion and a commonly occurring -263G/C polymorphism in the promoter region of the human AA-NAT gene. However, we found that -263G/C variant had no effect on the overnight 6-OHMS excretion. In this study, individual genotyping for -263G/C was determined by denaturing high performance liquid chromatography (DHPLC) and confirmed by sequencing. The overnight urinary 6-hydroxymelatonin sulfate (6-OHMS) excretion was quantified by enzyme-linked immunosorbent assay (ELISA).
Ying
Grace Wang
GW
Department of Pharmacology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore.
Lee
Caroline Guat Lay
CG
Lee
Edmund Jon Deoon
EJ
D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D001191:Arylamine N-Acetyltransferase; D008550:Melatonin; C012365:6-hydroxymelatonin