title: Identification of a region within SEL1L protein required for tumour growth inhibition.
abstract: The ectopic expression of the entire SEL1L cDNA significantly reduces the proliferate activity and aggressive behavior of the human breast carcinoma cell line MCF-7. However, the mechanism responsible for this biological effect remains unclear. A study was initiated to define those regions within SEL1L conferring such antiproliferative properties. Here we report that the region between amino acid residues 659-794 contains a functionally relevant domain since a deletion mutant impairs SEL1L's ability to suppress tumor cell growth. This region contains the Hrd3 motif, tetratricopeptide (TPR)-like SEL1 repeat, a transmembrane region, and a proline-rich tail.
authors.affiliation: Institute for Biomedical Technologies (National Research Council), Milan, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Cattaneo
authors.forename: Cristina
authors.initials: C
authors.lastname: Canton
authors.forename: Alberto
authors.initials: A
authors.lastname: Albertini
authors.forename: Ida
authors.initials: I
authors.lastname: Biunno
mesh_terms: D002455:Cell Division / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D011506:Proteins; C117384:SEL1L protein, human