Neonatal deafening causes changes in Fos protein induced by cochlear electrical stimulation.
The influence of neonatal deafness on cochlear electrically evoked Fos expression in the auditory brainstem was examined. Newborn rats were deafened by systemic injection of kanamycin, 1 mg/g daily for 12 days. At 4, 5, 6 or 8 weeks of age, these animals received cochlear electrical stimulation with a basal monopolar electrode for 90 minutes. Age-matched untreated control animals received similar stimulation. Experimental and control animals were assessed for spiral ganglion cell densities and Fos immunoreactive staining in the central nucleus of the inferior colliculus. Spiral ganglion cell assessments showed significant decreases in spiral ganglion cell densities in deafened rats compared to age-matched controls, at 5 weeks of age in lower turns and 6 and 8 weeks in all turns. Cochlear electrical stimulation induced Fos immunoreactive staining in the nucleus of auditory brain stem neurons in treatment and control groups. A significantly greater number of Fos immunoreactive neurons was found in the contralateral central nucleus of inferior colliculus in 5, 6 and 8 week old deafened animals compared to age-matched controls. The increases were larger with a longer duration of deafness. These results suggest that there are changes in auditory processing as a consequence of neonatal deafness.
Nagase
Shigeyo
S
Kresge Hearing Research Institute, Department of Otolaryngology, University of Michigan, Ann Arbor, MI 48109, USA.
Mukaida
Masahiro
M
Miller
Josef M
JM
Altschuler
Richard A
RA
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001306:Auditory Pathways / Q000503:physiopathology*; D003051:Cochlea / Q000293:injuries / Q000601:surgery; D003054:Cochlear Implants / Q000592:standards; D003638:Deafness / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D004558:Electric Stimulation; D007839:Functional Laterality / Q000502:physiology; D007150:Immunohistochemistry; D007245:Inferior Colliculi / Q000378:metabolism / Q000503:physiopathology*; D007612:Kanamycin; D007805:Language Development Disorders / Q000503:physiopathology; D009410:Nerve Degeneration / Q000209:etiology / Q000503:physiopathology*; D009431:Neural Conduction / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000473:pathology* / Q000503:physiopathology
D016760:Proto-Oncogene Proteins c-fos; D007612:Kanamycin