title: [Evaluation of interaction between drugs and ordered phospholipid membrane by immobilized artificial membrane chromatography].
abstract: OBJECTIVE
  To investigate the interaction between drugs and ordered phospholipid membrane using immobilized artificial membrane chromatography (IAMC).
  
  
  METHODS
  IAMC was used to determine the interaction drugs with phospholipid membrane, expressed as membrane affinity (lg kIAM). An n-octanol/buffer system was also employed as the reference hydrophobicity (lg Do/w,7.4).
  
  
  RESULTS
  Within the range of used acetonitrile percentages (phi) 0-30% in mobile phase, retention index (lg kIAM) showed excellent correlation with phi. Intercepts of fitted straight lines between lg kIAM and phi were comparable but slopes were much different for the three organic modifiers (acetonitrile, ethanol and methanol). Effects by adding CH2 substituent on lipophilicity difference (delta lg kIAM and delta lg Do/w,7.4) were similar for p-hydroxyl benzoic methyl ester to butyl ester, whereas different for p-hydroxylbenzoic acid to methyl ester.
  
  
  CONCLUSIONS
  IAMC system is a convenient, efficient and rapid tool for determining membrane interaction.
authors.affiliation: Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. sunjin66@21cn.com
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: Zhong-gui
authors.initials: ZG
authors.lastname: He
authors.forename: Shu-jun
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D008567:Membranes, Artificial; D000442:Octanols / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D000442:Octanols; D004364:Pharmaceutical Preparations; D010743:Phospholipids