A myosin inhibitor impairs auxin-induced cell division.
The role of myosins for auxin-induced cell division was probed using the inhibitor 2,3-butanedione monoxime in the tobacco cell line VBI-0, where cell elongation and division are axially aligned under the control of auxin. A morphometric analysis revealed that cell division is blocked in a dose-dependent manner, whereas cell expansion continued. In addition, the polarity of terminal cells was impaired resulting in malformed, pear-shaped cells. Early effects of the inhibitor are aberrant features of the cytoarchitecture including a block of vesicle transport, a diffuse broadening of cross walls, and the disorganization of the actin cytoskeleton. The findings are discussed with respect to a possible role of myosins as link between vesicle flow and signal control of cell division.
Holweg
Carola
C
Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, 79104 Freiburg, Federal Republic of Germany.
Honsel
Anne
A
Nick
Peter
P
D000199:Actins / Q000187:drug effects / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D003931:Diacetyl / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000502:physiology; D009218:Myosins / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology*
D000199:Actins; D007210:Indoleacetic Acids; D009218:Myosins; D003931:Diacetyl