title: In vivo conjugation of nasal lavage proteins by hexahydrophthalic anhydride.
abstract: Hexahydrophthalic anhydride (HHPA), an industrially important chemical, is a highly allergenic compound. The aim of this work was to identify proteins in nasal lavage fluid (NLF) that form adducts with HHPA. Such bindings may induce production of specific immunoglobulin E (IgE) or affect physiological mechanisms of the proteins. NLF was obtained from HHPA-exposed volunteers, workers and exposed guinea pigs. HHPA-binding proteins were visualized with immunoblotting using a polyclonal antiserum against HHPA. The proteins were excised from sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, digested with trypsin and identified by tandem mass spectrometry (MS/MS) and database searches. The antiserum was found to be specific for HHPA-bound proteins. In vivo formed HHPA-binding proteins in humans were identified as antileukoproteinase, immunoglobulin G (IgG), immunoglobulin A (IgA), serum albumin and lactoferrin. In addition, several proteins binding to HHPA were found in NLFs from guinea pigs but these could not be identified from database searches. Hypotheses for development of airways diseases by adduction of this allergenic compound to the NLF proteins in humans were established.
authors.affiliation: Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, Lund University Hospital, SE-221 85, Lund, Sweden. gunvor.johannesson@ymed.lu.se
authors.forename: Gunvor
authors.initials: G
authors.lastname: Johannesson
authors.forename: Christian
authors.initials: C
authors.lastname: Lindh
authors.forename: Jörn
authors.initials: J
authors.lastname: Nielsen
authors.forename: Birgitta
authors.initials: B
authors.lastname: Björk
authors.forename: Seema
authors.initials: S
authors.lastname: Rosqvist
authors.forename: Bo A G
authors.initials: BA
authors.lastname: Jönsson
mesh_terms: D000818:Animals; D004853:Epoxy Resins / Q000378:metabolism* / Q000494:pharmacology*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D010796:Phthalic Anhydrides / Q000378:metabolism* / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D011506:Proteins / Q000378:metabolism*
chemical_list: D004853:Epoxy Resins; D010796:Phthalic Anhydrides; D011506:Proteins; C005752:araldite