Liquid chromatographic determination of fumonisins B1, B2, and B3 in corn silage.
Corn silage was dried, ground, and then extracted with 0.1 M ethylenediaminetetraacetic acid. The filtrate was applied to a FumoniTest immunoaffinity column. Fumonisins were derivatized with naphthalene-2,3-dicarboxaldehyde, separated on a C(18) liquid chromatographic column, and detected by fluorescence. The detection limits for fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 50, 25, and 25 ng/g of dried silage, respectively. Recoveries of fumonisin B(1), fumonisin B(2), and fumonisin B(3) from wet and dried corn silage spiked over the range of 100-5000 ng/g averaged 91-106%. The method was applied to corn silage samples collected from the midwestern area of the United States during 2001-2002. Of 89 corn silage samples, fumonisin B(1), fumonisin B(2), and fumonisin B(3) were found in 86 (97%), 64 (72%), and 51 (57%) of the samples. The mean positive levels of fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 615, 93, and 51 ng/g, respectively, in dried silage. This suggests that fumonisins may be frequent low level contaminants in corn silage.
Kim
Eun-Kyung
EK
Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 North University Street, Peoria, Illinois 61604, USA.
Maragos
Chris M
CM
Kendra
David F
DF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis*; D009281:Naphthalenes; D012820:Silage / Q000032:analysis*; D012997:Solvents; D003313:Zea mays / Q000737:chemistry*
D037341:Fumonisins; D009281:Naphthalenes; D012997:Solvents; C056934:fumonisin B2; C073063:fumonisin B3; C056933:fumonisin B1; C027717:2,3-naphthalenedicarboxaldehyde