[Influence of inhibitors of nitric oxide synthase and L-arginine on the survival rate in a rat model of traumatic shock].
OBJECTIVE
To investigate the influence of inhibitors of nitric oxide synthase (NOS) and L-arginine on the survival rate in a rat model of traumatic shock.


METHODS
A rat model of traumatic shock was established by fracturing the posterior limb of the rat. Nomega-nitro-L-arginine-methyl ester (L-NAME, non-selective NOS inhibitor, 10 mg/kg), amino- guanidine (AG, selective inducible nitric oxide synthase (iNOS) inhibitor, 100 mg/kg) and L-arginine (L-Arg, the precursor of nitric oxide (NO) synthesis 100 mg/kg) were injected intravenously during resuscitation,survival time and survival rate were observed.


RESULTS
There were no significant changes in survival time and 24-hour survival rate between L-NAME ((23.80+/-9.09) hours and 40 percent) and control group (18.78+/-4.64)hours and 10 percent, both P>0.05); the survival time of AG group (28.72+/-6.25) hours and L-Arg group (30.64+/-8.77) hours prolonged apparently (both P<0.01), and 24-hour survival rate was also increased (both 80 percent, both P<0.01).


CONCLUSIONS
Selective iNOS inhibitor AG and L-Arg exert beneficial effects on after traumatic shock rats.
Huang
Zong-hai
ZH
Department of General Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong, China.
Feng
Hao-miao
HM
Huang
Xu-liang
XL
Sun
Ying-gang
YG
Lin
Hong-wu
HW
D000818:Animals; D001120:Arginine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D019331:NG-Nitroarginine Methyl Ester / Q000627:therapeutic use; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012774:Shock, Traumatic / Q000188:drug therapy* / Q000401:mortality; D015996:Survival Rate; D016896:Treatment Outcome
D004791:Enzyme Inhibitors; D001120:Arginine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester