The mechanism of Polydatin in shock treatment.
Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM-1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC-EC adhesion, increase [Ca2+]i in MC with enhancement of MC contraction extent, activate KATP channels of VSMC, and decrease pHi value and [Ca2+]i of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.
Zhao
Ke-Seng
KS
Department of Pathophysiology, First Military Medical University, Guangzhou 510515, China. zhaoks@fimmu.com
Jin
Chunhua
C
Huang
Xuliang
X
Liu
Jie
J
Yan
Wen Sheng
WS
Huang
Qiaobing
Q
Kan
Wenhong
W
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008833:Microcirculation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D018408:Patch-Clamp Techniques; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D012771:Shock, Hemorrhagic / Q000188:drug therapy*; D012772:Shock, Septic / Q000188:drug therapy / Q000503:physiopathology; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D005960:Glucosides; D015221:Potassium Channels; D013267:Stilbenes; D018799:Intercellular Adhesion Molecule-1; D014700:Verapamil; C058229:polydatin