title: Telomerase activity in chronic inflammatory bowel disease.
abstract: Patients with ulcerative colitis have an increased risk of developing colorectal cancer. Telomerase appears to be associated with cellular immortality and might serve as a diagnostic and prognostic marker in carcinogenesis. To test this hypothesis we measured telomerase by a score from 0 (no activity) to 4 (very high activity) in specimens obtained surgically from seven patients with adenocarcinoma of the colon. Intraindividual comparison was made among normal tissue (mean score +/- SD: 0.7 +/- 1.0), tissues adjacent to the tumor (2.7 +/- 0.8), and the tumor center (3.0 +/- 1.0). In addition, from 18 patients with ulcerative colitis, 10 patients with Crohn's disease, and 14 patients without chronic inflammatory bowel disease, biopsies were collected from normal and inflamed areas of the colon. Independent of the duration (0-32 years), grade, and location of the diseases, the telomerase activities were comparable, ranging from 0.4 to 1.0 in ulcerative colitis and from 0.3 to 0.6 in Crohn's disease and averaging 0.4 in controls. Apparently low telomerase activities are present in the mucosa of all patients and the enzyme is not yet upregulated in the potentially premalignant state of active ulcerative colitis, dismissing its prognostic value as an early tumor marker for this disorder.
authors.affiliation: Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Auerbachstr. 112, D-70376 Stuttgart, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Kleideiter
authors.forename: Ulrike
authors.initials: U
authors.lastname: Friedrich
authors.forename: Alexandra
authors.initials: A
authors.lastname: Möhring
authors.forename: Siegfried
authors.initials: S
authors.lastname: Walker
authors.forename: Emil
authors.initials: E
authors.lastname: Höring
authors.forename: Karlernst
authors.initials: K
authors.lastname: Maier
authors.forename: Peter
authors.initials: P
authors.lastname: Fritz
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Thon
authors.forename: Ulrich
authors.initials: U
authors.lastname: Klotz
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic; D003093:Colitis, Ulcerative / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D019098:Telomerase / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D019098:Telomerase