title: Molecular diagnosis of cerebral aspergillosis by sequence analysis with panfungal polymerase chain reaction.
abstract: The authors describe a patient with osteosarcoma in whom a brain abscess developed after autologous peripheral stem cell transplantation. Serologic markers of fungal infection were negative, but fungal DNA was detected in the cerebrospinal fluid (CSF) by panfungal polymerase chain reaction (PCR) assay using primers derived from fungal 18S ribosomal RNA (rRNA) genes. The sequence of PCR products on the panfungal assay was identical to the 18S rRNA genes of Aspergillus species. The combination of sequence analysis and panfungal PCR assay could be useful in the diagnosis of cerebral aspergillosis.
authors.affiliation: Department of Pediatrics, National Defense Medical College, 3-2 Namik Tokorozawa, Saitama 359-8513, Japan. hotkomatsu@hotmail.com
authors.forename: Haruki
authors.initials: H
authors.lastname: Komatsu
authors.forename: Tomoo
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Ayano
authors.initials: A
authors.lastname: Inui
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Horiuchi
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Hashizume
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sogo
authors.forename: Isao
authors.initials: I
authors.lastname: Sekine
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D004271:DNA, Fungal / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D020953:Neuroaspergillosis / Q000175:diagnosis*; D012516:Osteosarcoma / Q000150:complications / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D014182:Transplantation, Autologous
chemical_list: D004271:DNA, Fungal