title: The cellular responses of articular cartilage to sharp and blunt trauma.
abstract: OBJECTIVE
  To determine the response of immature articular cartilage to both sharp and blunt trauma in terms of cell death, cell proliferation and matrix synthesis.
  
  
  METHODS
  Blunt wounds were made with a trephine in full depth immature bovine articular cartilage explants which were cut in half through the center of the trephine wound with a sharp scalpel to produce blunt and sharp trauma on the same explant. Explants were maintained in culture for up to 10 days. Prior to fixation at days 2, 5 and 10, medium was supplemented with 10 microCi ml-1 35S-sulphate, [3H]-proline or [3H]-thymidine for 24h to assess matrix synthesis and cell proliferation. Cell death was assessed using a Live/Dead label.
  
  
  RESULTS
  In the case of blunt wounds, a band of cell death was observed adjacent to the lesion edge. Microautoradiography demonstrated little radiolabel incorporation and, therefore, no new matrix synthesis or cell proliferation within this region. In contrast, wounds made with a sharp scalpel showed restricted cell death, with radiolabel incorporation adjacent to the lesion edge at all time points. This demonstrated not only chondrocyte proliferation and new matrix synthesis at the wound margin, but also an up-regulation of matrix synthesis adjacent to the lesion edge.
  
  
  CONCLUSIONS
  In terms of clinical relevance, the use of sharp precise instruments during the surgical management of cartilage defects may be necessary to reduce cell death and promote matrix elaboration at the lesion edge in order to facilitate successful integration.
authors.affiliation: Cardiff School of Biosciences, Cardiff Institute of Tissue Engineering and Repair, Museum Avenue,PO Box 911, CF10 3US, Cardiff, UK.
authors.forename: S N
authors.initials: SN
authors.lastname: Redman
authors.forename: G P
authors.initials: GP
authors.lastname: Dowthwaite
authors.forename: B M
authors.initials: BM
authors.lastname: Thomson
authors.forename: C W
authors.initials: CW
authors.lastname: Archer
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001345:Autoradiography / Q000379:methods; D002358:Cartilage, Articular / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D019902:Chondrocytes / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis; D046508:Culture Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D011392:Proline; D011509:Proteoglycans / Q000096:biosynthesis; D013525:Surgical Instruments; D013936:Thymidine; D015854:Up-Regulation; D014949:Wounds, Nonpenetrating / Q000378:metabolism / Q000503:physiopathology; D014950:Wounds, Penetrating / Q000378:metabolism / Q000503:physiopathology
chemical_list: D011509:Proteoglycans; D003094:Collagen; D011392:Proline; D013936:Thymidine