title: Immunohistochemical analysis of centromere protein F expression in buccal and gingival squamous cell carcinoma.
abstract: Centromere protein F (CENP-F) expression (localization and characteristics) in relation to tumor clinicopathological parameters was immunohistochemically examined and evaluated in 47 archival biopsy specimens of buccal and gingival squamous cell carcinomas (SCC). Centromere protein F expression was detected in 79% of the samples. An increase in the labeling index (LI) with WHO grading was obtained (P < 0.05). Correlations were obtained between the CENP-F LI and tumor size (P < 0.05). Immunoelectron microscopy showed CENP-F nuclear staining as punctate or fine dots. The present study shows that CENP-F expression and detection of a more specific cell subpopulation presents a theoretical advantage for the analysis of the precise cell cycle of G2 to M cells, compared to Ki-67.
authors.affiliation: Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. tatampa@tmd.ac.jp
authors.forename: Renelson L
authors.initials: RL
authors.lastname: Esguerra
authors.forename: Lin
authors.initials: L
authors.lastname: Jia
authors.forename: Tomoatsu
authors.initials: T
authors.lastname: Kaneko
authors.forename: Kei
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Norihiko
authors.initials: N
authors.lastname: Okada
authors.forename: Minoru
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D056741:Cyclin D; D016213:Cyclins / Q000378:metabolism; D005260:Female; D005881:Gingiva / Q000378:metabolism / Q000473:pathology*; D005887:Gingival Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology*; D009367:Neoplasm Staging; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D002868:Chromosomal Proteins, Non-Histone; D056741:Cyclin D; D016213:Cyclins; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; C094772:centromere protein F