title: Suppressive activity of macrolide antibiotics on nitric oxide production from nasal polyp fibroblasts in vitro.
abstract: The influence of macrolide antibiotics on nitric oxide (NO) generation was examined using human nasal polyp fibroblasts (NPFs) in vitro. Addition of roxithromycin (RXM) at a concentration of > 7.5 microg/ml to cell cultures was shown to suppress NO production in response to stimulation with 25.0 ng/ml tumor necrosis factor (TNF)-alpha. However, jyosamycin (JM) did not suppress NO production from NPFs induced by TNF-alpha stimulation in vitro, even when added to cell cultures at a concentration of 20.0 microg/ml. We then examined the influence of RXM on inducible nitric oxide synthase (iNOS) mRNA expression in NPFs. Addition of RXM at a dose of 7.5 microg/ml to cell cultures caused reduction of iNOS mRNA expression, which was enhanced by TNF-alpha stimulation in vitro.
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan. asanok@med.showa-u.ac.jp
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Asano
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Kamakazu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hisamitsu
authors.forename: Harumi
authors.initials: H
authors.lastname: Suzaki
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D015570:Josamycin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D015575:Roxithromycin / Q000008:administration & dosage / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015575:Roxithromycin; D009569:Nitric Oxide; D015570:Josamycin