title: Regulation of matrix metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1 by dichloroacetic acid in human fibroblasts from normal peritoneum and adhesions.
abstract: OBJECTIVE
  To examine the role of stimulation of aerobic metabolism on the differential expression of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), which are differentially regulated in fibroblasts isolated from normal human peritoneum and adhesions.
  
  
  METHODS
  Tissue culture study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Human fibroblasts cultures from normal peritoneum and adhesions that were exposed to dichloroacetic acid (DCA; 0 and 100 microg/mL) for 24 hours under normal and hypoxic conditions.
  
  
  METHODS
  Real-time reverse-transcription polymerase chain reaction of MMP-1, TIMP-1, and beta-actin.
  
  
  RESULTS
  Dichloroacetic acid stimulated peritoneal fibroblast MMP-1 mRNA expression under normoxic conditions; this stimulation was lost during hypoxia. In adhesion fibroblasts, DCA increased MMP-1 mRNA expression; this effect was reversed by hypoxia. Expression of TIMP-1 mRNA was insignificantly increased by DCA in normal peritoneal and adhesion fibroblasts under normoxic conditions; however under hypoxic conditions, DCA reduced TIMP-1 mRNA expression from both.
  
  
  CONCLUSIONS
  Regulation of metabolic activity of peritoneal cells may provide a target for future interventions for reduction of development of postoperative adhesions, particularly as it relates to healing of peritoneal sites that did not previously have adhesions as opposed to sites that underwent lysis of preexistent adhesions.
authors.affiliation: Department of Obstetrics and Gynecology, Wayne State University/Detroit Medical Center, Hutzel Hospital, Detroit, Michigan 48201, USA. mdiamond@med.wayne.edu
authors.forename: Michael P
authors.initials: MP
authors.lastname: Diamond
authors.forename: Eslam
authors.initials: E
authors.lastname: El-Hammady
authors.forename: Rona
authors.initials: R
authors.lastname: Wang
authors.forename: Ghassan
authors.initials: G
authors.lastname: Saed
mesh_terms: D015687:Cell Hypoxia; D002478:Cells, Cultured; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D000267:Tissue Adhesions / Q000473:pathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D003999:Dichloroacetic Acid; D020781:Matrix Metalloproteinase 1