Antipyretic effect of acetaminophen by inhibition of glutamate release after staphylococcal enterotoxin A fever in rabbits.
The present study was designed to determine whether the inhibition of glutamate release in organum vasculosum laminae terminalis (OVLT) of rabbit brain by acetaminophen might be protective in a whole-animal model of staphylococcal enterotoxin A (SEA) fever. Unanesthetized rabbits were administered intravenously with SEA, and both colonic temperature (Tco) and glutamate release in OVLT were measured simultaneously. The glutamate release in OVLT was measured with a microdialysis probe previously implanted. Both the Tco and glutamate release in OVLT were simultaneously increased following intravenous administration of SEA. The SEA-induced rise in both the Tco and the levels of glutamate release in OVLT were suppressed by pretreatment with intravenous injection of acetaminophen (1, 5 or 10 mg/kg). Furthermore, treatment of OVLT with acetaminophen (50-150 microg) attenuated the fever-like hyperthermia induced by intra-OVLT injection of glutamate. Our results show acetaminophen may reduce glutamate release in OVLT and result in antipyresis.
Huang
Wu-Tein
WT
Department of Health Care Administration, Di Wan College of Management, Madou, Tainan 721, Taiwan.
Wang
Jhi-Joung
JJ
Lin
Mao-Tsun
MT
D000082:Acetaminophen / Q000494:pharmacology*; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions / Q000502:physiology; D004768:Enterotoxins; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D018698:Glutamic Acid / Q000378:metabolism* / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011817:Rabbits
D018712:Analgesics, Non-Narcotic; D004768:Enterotoxins; D000082:Acetaminophen; C026066:enterotoxin A, Staphylococcal; D018698:Glutamic Acid