title: Genotyping of Toxoplasma gondii isolates from chickens from India.
abstract: The present study was undertaken to isolate and genotype Toxoplasma gondii from free-range chickens (Gallus domesticus) from villages in Maharashtra and Tamil Nadu states of central and south India, respectively. Blood, heart, and brain from a total of 741 chickens were examined for T. gondii infection. Antibodies to T. gondii, as assayed with the modified agglutination test (MAT >or = 1:5) were found in 133 (17.9%) chickens. Hearts and brains of 186 chickens were bioassayed in mice. Additionally, hearts and/or brains of most of the seronegative (MAT < 1:5) chickens were fed to 20 T. gondii-free cats, while 32 seropositive chickens (MAT 1:5) were fed to 3 cats. T. gondii was not isolated from any of the chickens by mouse bioassay. Five of the cats that were fed seronegative chickens shed oocysts, while isolates were not obtained from any of the other cats fed seropositive chickens. These five isolates, along with the two that were previously isolated in India through cat bioassay, were genetically analyzed. Genotyping using the SAG 2 locus indicated that two isolates were type II and five were type III. Microsatellite analysis revealed allelic differences between and within the lineages. This is the first report of genetic characterization of any T. gondii isolate from India.
authors.affiliation: United States Department of Agriculture, Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville, MD 20705-2350, USA. kumar@anri.barc.usda.gov
authors.forename: C
authors.initials: C
authors.lastname: Sreekumar
authors.forename: D H
authors.initials: DH
authors.lastname: Graham
authors.forename: E
authors.initials: E
authors.lastname: Dahl
authors.forename: T
authors.initials: T
authors.lastname: Lehmann
authors.forename: M
authors.initials: M
authors.lastname: Raman
authors.forename: D P
authors.initials: DP
authors.lastname: Bhalerao
authors.forename: M C B
authors.initials: MC
authors.lastname: Vianna
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay / Q000662:veterinary; D001921:Brain / Q000469:parasitology; D002415:Cats; D002645:Chickens; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D006321:Heart / Q000469:parasitology; D007194:India; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000469:parasitology*; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan