title: T-complex polypeptide-1 interacts with the erythrocyte cytoskeleton in response to elevated temperatures.
abstract: Chaperonins are double ring complexes composed of highly conserved 60-kDa protein subunits that are divided into two subgroups. Group II chaperonins are found in archaea and the cytoplasm of eukarya and are believed to function like other chaperonins as part of a protein folding system. We report here that human erythrocytes contain the group II chaperonin T-complex polypeptide 1 (TCP-1) and that this complex translocates from the cytoplasm to the cytoskeleton in response to heat treatment in the absence of overt cell damage. Identification as TCP-1 was determined by immunodetection for TCP-1alpha and corroborated by mass spectroscopy peptide sequencing. Direct visualization by immunofluorescence confirmed peripherally localized TCP-1 in response to heat treatment. Temperatures ranging from 37-50 degrees C were demonstrated to have distinct kinetic profiles of induced translocation. Heat-induced binding was shown by Triton shell analysis to be specifically associated with the cytoskeletal proteins. Furthermore, the binding was reversible following removal of the stimulatory condition. A stabilizing process is hypothesized based on the known interactions of chaperonins.
authors.affiliation: LifeCell Corporation, Branchburg, New Jersey 08876, USA. cwagner@lifecell.com
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Wagner
authors.forename: Irene Y
authors.initials: IY
authors.lastname: Lu
authors.forename: Michael H
authors.initials: MH
authors.lastname: Hoffman
authors.forename: Wendell Q
authors.initials: WQ
authors.lastname: Sun
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Trent
authors.forename: Jerome
authors.initials: J
authors.lastname: Connor
mesh_terms: D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D011485:Protein Binding; D021381:Protein Transport; D013696:Temperature
chemical_list: D056404:Chaperonin Containing TCP-1; D018833:Chaperonins