title: Electrodes modified with monoolein cubic phases hosting laccases for the catalytic reduction of dioxygen.
abstract: An enzyme-catalyzed process has been used for dioxygen monitoring. The enzymes were two different laccases (p-diphenol:dioxygen oxidoreductases), chosen as catalysts for dioxygen reduction. The laccases were immobilized in a liquid crystalline cubic phase formed with monoolein. The structures of the cubic phases, both with and without enzymes, were established using small-angle X-ray scattering. The catalytic reduction of dioxygen was performed using a glassy carbon electrode modified with cubic phases containing the enzymes. The modified electrode was used as a dioxygen sensing system, based on the increasing reduction current of a suitable electrochemical probe in the presence of dioxygen.
authors.affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland, and Equipe Physico-chimie des Colloïdes, UMR 7565 CNRS, Faculté des Sciences, Université Henri Poincaré Nancy 1, BP 239, 54506 Vandoeuvre-lès-Nancy, Cedex, France.
authors.forename: Paweł
authors.initials: P
authors.lastname: Rowiński
authors.forename: Renata
authors.initials: R
authors.lastname: Bilewicz
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Stébé
authors.forename: Ewa
authors.initials: E
authors.lastname: Rogalska
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry; D005989:Glycerides / Q000737:chemistry*; D006873:Hydroquinones / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis*; D011803:Quinine / Q000031:analogs & derivatives* / Q000737:chemistry; D013696:Temperature
chemical_list: D005989:Glycerides; D006873:Hydroquinones; C070445:quininone; D011803:Quinine; C005953:monoolein; D042845:Laccase; D010100:Oxygen; C031927:hydroquinone