title: [Biologic significance of thyroid transcription factor-1 and surfactant protein expression in pulmonary sclerosing hemangioma].
abstract: OBJECTIVE
  To investigate the biologic significance of thyroid transcription factor-1 (TTF-1) and surfactant protein A (SP-A) and SP-B expression in pulmonary sclerosing hemangioma (PSH).
  
  
  METHODS
  TTF-1, SP-A, SP-B, epithelial membrane antigen (EMA), pancytokeratin (AE(1)/AE(3)), vimentin, CK7, CK5/6, calretinin, S-100, neuron specific enolase (NSE), synaptophysin (Syn), chromogranin A (CgA), CD(34), Factor VIII and smooth muscle actin (SMA) in 42 patients with PSH were examined with immunohistochemistry, while samples from 10 patients were also observed by electron microscope.
  
  
  RESULTS
  Histopathologically, PSH mainly consisted of both surface lining cuboidal cells and pale polygonal cells. Both of them were stained with TTF-1, EMA and vimentin, whereas SP-A, SP-B, pancytokeratin and CK7 were only positive in surface lining cuboidal cells. Syn, NSE, S-100 and CgA showed scattered positivity in these cells. There was no significant difference in the expressions of TTF-1 and EMA between these two cell types (P > 0.05), whereas the difference was significant in the expression of vimentin (P < 0.01). The ultrastructural features cannot differentiate these two cells by electron microscope.
  
  
  CONCLUSIONS
  It is suggested that PSH is derived from primitive respiratory epithelium, and both surface lining cuboidal cells and pale polygonal cells were entity cells of the tumor. Examination of different immunohistochemical markers including TTF-1, SP-A, SP-B, pancytokeratin, EMA and vimentin is helpful in the diagnosis and differential diagnosis of PSH.
authors.affiliation: Department of Pathology, Shanghai Pulmonary Hospital, Shanghai 200433, China.
authors.forename: Xiang-hua
authors.initials: XH
authors.lastname: Yi
authors.forename: Rong-xuan
authors.initials: RX
authors.lastname: Zhang
authors.forename: Jing-yun
authors.initials: JY
authors.lastname: Shi
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Ji
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006391:Hemangioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis; D037701:Pulmonary Surfactant-Associated Protein B / Q000032:analysis; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000032:analysis; D014746:Vimentin / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D018396:Mucin-1; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D014746:Vimentin