Quantification of metallothionein-like proteins in the mussel Mytilus galloprovincialis using RP-HPLC fluorescence detection.
A HPLC-fluorescence method, using the fluorophore SBD-F (ammonium-7-fluorobenz-2-oxa-1,3-diazole-4-sulfonate), was adapted for the quantification of metallothioneins and their isoforms from the Moroccan mussel Mytilus galloprovincialis. The method was first optimized using a rabbit liver metallothionein. The effects of EDTA, tris(2-carboxyethyl)phosphine, and SBD-F on the labeling efficiency were studied. The optimized method was then applied to evaluate the amount of metallothionein in the mussels either exposed to cadmium in the laboratory or collected from the Casablanca coast, Morocco. The concentrations of metallothioneins measured in the field samples describe the degree of contamination of the sites and are reflected by distinct isoform patterns.
El Ghazi
Issam
I
Martin-Luther-University Halle-Wittenberg, Department Biochemistry/Biotechnology, Division of Ecology and Plant-Biochemistry, Kurt-Mothes-Strasse 3, 06120 Halle, Germany.
Menge
Sieglinde
S
Miersch
Juergen
J
Chafik
Abdelghani
A
Benhra
Ali
A
Elamrani
M Khalid
MK
Krauss
Gerd-Joachim
GJ
D000818:Animals; D049872:Bivalvia; D002104:Cadmium / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D004784:Environmental Monitoring; D005453:Fluorescence; D008668:Metallothionein / Q000032:analysis*; D011817:Rabbits; D014018:Tissue Distribution; D014873:Water Pollutants / Q000633:toxicity
D014873:Water Pollutants; D002104:Cadmium; D008668:Metallothionein