HB-GAM/Pleiotrophin and Midkine are differently expressed and distributed during retinoic acid-induced neural differentiation of P19 cells.
HB-GAM/Pleiotrophin and Midkine (MK) are developmentally-regulated proteins with putative functions during cell growth and differentiation. Using the P19 cell which is a model to study the events associated with early development, we examined the expression and cellular localization of HB-GAM and MK during neural differentiation of P19 cells induced by retinoic acid (RA). The temporal expressions of HB-GAM and MK transcripts and both the levels and cellular localizations of the corresponding proteins appeared dramatically different. MK mRNA, already expressed in untreated P19 cells, was transiently increased by exposure to RA and then largely down regulated. More interestingly, HB-GAM which was not detected in untreated P19 cells was strongly expressed after 2 days of RA treatment and this expression persists throughout the duration of the culture suggesting that it could be involved in different aspects of this differentiation process.
Brunet-de Carvalho
Nicole
N
INSERM U 440/ UPM, Signalisation et Différenciation Cellulaires dans les Systèmes Nerveux et Musculaire, Institut du Fer à Moulin, 17 rue du Fer à Moulin F-75005 Paris, France.
Raulais
Daniel
D
Rauvala
Heikki
H
Souttou
Boussad
B
Vigny
Marc
M
D000818:Animals; D015152:Blotting, Northern; D018236:Carcinoma, Embryonal / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007641:Keratolytic Agents / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D000078792:Midkine; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D053670:Syndecan-3; D013997:Time Factors; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*
D002352:Carrier Proteins; D016207:Cytokines; D018076:DNA, Complementary; D007641:Keratolytic Agents; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; D011994:Recombinant Proteins; C508301:SDC3 protein, human; C508302:Sdc3 protein, mouse; D053670:Syndecan-3; C066845:pleiotrophin; D000078792:Midkine; D014212:Tretinoin; D004247:DNA; D002795:Choline O-Acetyltransferase