[Effect and mechanism of arsenic trioxide on chemosensitivity of human lung adenocarcinoma cells].
OBJECTIVE
To study the apoptosis and chemosensitive effect of As(2)O(3) on human lung adenocarcinoma cell line A549.


METHODS
MTT colorimetric assay, immunohistochemical method, flow cytometry and RT-PCR were applied to observe the different effects induced by As(2)O(3) with different concentrations on A549 cells.


RESULTS
1, 2 micro mol/L As(2)O(3) increased Fas expression and inhibited the expression of bcl-2, MRP and LRP; G(1) arrest was observed and the cells were more sensitive to cisplatin. A549 cells in the presence of 5 micro mol/L As(2)O(3) showed growth inhibition and up-regulation of bcl-2, MRP and LRP expressions; but their chemosensitivity to cisplatin did not change.


CONCLUSIONS
As(2)O(3) in low concentrations can increase the chemosensitivity of A549 cells by regulating the expression of apoptosis genes, anti-apoptosis genes and drug-resistant genes and affecting the cell cycle progress.
Li
Huai-chen
HC
Department of Respiratory Medicine, Shandong Provincial Hospital, Jinan 250021, China.
Wang
Chun-xia
CX
Huang
Chen
C
Wang
Lan-fang
LF
Mu
Xiao-yan
XY
Jiang
Shu-juan
SJ
Lei
Mao-lu
ML
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D010087:Oxides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein
D000970:Antineoplastic Agents; D000077237:Arsenic Trioxide; D001152:Arsenicals; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins; D010087:Oxides; D019253:Proto-Oncogene Proteins c-bcl-2; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein