Effect of venipuncture site on hematologic and serum biochemical parameters in marginated tortoise (Testudo marginata).
Blood samples were obtained from the dorsal coccygeal vein and the brachial vein of five adult (four females and one male) and two subadult males of marginated tortoise (Testudo marginata) and hematologic and biochemical parameters were compared. Significant differences were found for red blood cell count, hematocrit, hemoglobin concentration, total proteins, uric acid, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, calcium, and phosphorus, which were greater in the brachial vein samples. Hemodilution due to lymph was observed when collecting blood from the dorsal coccygeal vein, and it is thought to be the cause of the differences found. This research documented that the brachial vein is a more reliable and consistent venipuncture site than dorsal coccygeal vein in marginated tortoise.
López-Olvera
Jorge Ramón
JR
Servei d'Ecopatologia de Fauna Salvatge, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain.
Montané
Jordi
J
Marco
Ignasi
I
Martínez-Silvestre
Albert
A
Soler
Joaquim
J
Lavín
Santiago
S
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary; D001800:Blood Specimen Collection / Q000662:veterinary; D002118:Calcium / Q000097:blood; D004906:Erythrocyte Count / Q000662:veterinary; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D006454:Hemoglobins; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D018962:Phlebotomy / Q000379:methods / Q000662:veterinary*; D010758:Phosphorus / Q000097:blood; D011188:Potassium / Q000097:blood; D012016:Reference Values; D014426:Turtles / Q000097:blood*; D014680:Veins
D006454:Hemoglobins; D010758:Phosphorus; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D011188:Potassium; D002118:Calcium