title: Harvesting peripheral blood stem cells from healthy donors on 4th day of cytokine mobilization.
abstract: A pilot study was conducted to evaluate the efficacy of harvesting peripheral blood stem cells from normal healthy donors on day 4 after mobilization with G-CSF at 10 microg/kg for 4 days or a sequential combination of GM-CSF at 10 microg/kg for 2 days and G-CSF at 10 microg/kg for 2 more days. Harvesting over the target dose (>4 x 10(6) kg) of CD34+ cells based on the lst leukapheresis performed on day 4 was possible from 7 (53.8%) out of 13 donors. The 7 matched recipients all exhibited early engraftment, except for one who experienced transplant-related mortality, and no differences were observed with the recipients transplanted with stem cells harvested after a 5-6-day growth-factor (GF) treatment. Accordingly, harvesting from normal healthy donors on day 4 after mobilization treatment with a GF was found to be feasible for allogeneic peripheral blood stem cell transplantation and more economical in terms of the cost of the GF and the donor's commitment.
authors.affiliation: Department of Hematology/Oncology, Kyungpook National University Hospital, Daegu, South Korea.
authors.forename: Sang Kyun
authors.initials: SK
authors.lastname: Sohn
authors.forename: Jong Gwang
authors.initials: JG
authors.lastname: Kim
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Sung
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Jang Soo
authors.initials: JS
authors.lastname: Suh
authors.forename: Kun Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Kyu Bo
authors.initials: KB
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000008:administration & dosage*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis; D019520:Living Donors; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D010865:Pilot Projects; D011994:Recombinant Proteins; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor