title: Redesigning the folding energetics of a model three-helix bundle protein by site-directed mutagenesis.
abstract: Because of their limited size and complexity, de novo designed proteins are particularly useful for the detailed investigation of folding thermodynamics and mechanisms. Here, we describe how subtle changes in the hydrophobic core of a model three-helix bundle protein (GM-0) alter its folding energetics. To explore the folding tolerance of GM-0 toward amino acid sequence variability, two mutant proteins (GM-1 and GM-2) were generated. In the mutants, cavities were created in the hydrophobic core of the protein by either singly (GM-1; L35A variant) or doubly (GM-2; L35A/I39A variant) replacing large hydrophobic side chains by smaller Ala residues. The folding of GM-0 is characterized by two partially folded intermediate states exhibiting characteristics of molten globules, as evidenced by pressure-unfolding and pressure-assisted cold denaturation experiments. In contrast, the folding energetics of both mutants, GM-1 and GM-2, exhibit only one folding intermediate. Our results support the view that simple but biologically important folding motifs such as the three-helix bundle can reveal complex folding plasticity, and they point to the role of hydrophobic packing as a determinant of the overall stability and folding thermodynamic of the helix bundle.
authors.affiliation: Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
authors.forename: Dahabada H J
authors.initials: DH
authors.lastname: Lopes
authors.forename: Alex
authors.initials: A
authors.lastname: Chapeaurouge
authors.forename: Gavin A
authors.initials: GA
authors.lastname: Manderson
authors.forename: Jonas S
authors.initials: JS
authors.lastname: Johansson
authors.forename: Sérgio T
authors.initials: ST
authors.lastname: Ferreira
mesh_terms: D019791:Guanidine / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D011312:Pressure; D011489:Protein Denaturation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D011506:Proteins; D019791:Guanidine