title: A model of aluminum exposure associated with lipid peroxidation in rat brain.
abstract: We have developed a rat model to investigate the relationship between aluminum exposure and aluminum accumulation, and with oxidative damage in brain tissues. Intraperitoneal injections of aluminum lactate for 7 wk (the total aluminum dosage per rat was approx 100 mg) significantly increased aluminum levels in the brain. The concentration of lipid peroxidation products (thiobarbituric acid-reactive substances [TBARS]) also increased in the brain following aluminum lactate injections. No significant correlations between the concentrations of aluminum and of TBARS were found in the whole brain. Subcellular analysis revealed that aluminum lactate injections led to a significant increase in the concentration of aluminum in the mitochondrial fraction but had no significant effect on the concentration of peroxides in any subcellular fraction. These results suggest that aluminum accumulation induced by the aluminum lactate administration associates with the acceleration of lipid peroxidation in rat brain. Furthermore, these data indicate that the pro-oxidant effect of aluminum may be indirect and concentration independent. The experimental conditions used here provide an animal model of aluminum accumulation in the brain that should prove useful for further investigations of the mechanisms of aluminum neurotoxicity.
authors.affiliation: Department of Environmental Biology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogasawara
authors.forename: Eri
authors.initials: E
authors.lastname: Ohata
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishii
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Shinzo
authors.initials: S
authors.lastname: Tanabe
mesh_terms: D017607:Aluminum Compounds / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D007274:Injections, Intraperitoneal; D007773:Lactates / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017607:Aluminum Compounds; D007773:Lactates; C104223:aluminum lactate