title: DNA-templated self-assembly of protein and nanoparticle linear arrays.
abstract: Self-assembling DNA tiling lattices represent a versatile system for nanoscale construction. Self-assembled DNA arrays provide an excellent template for spatially positioning other molecules with increased relative precision and programmability. Here we report an experiment using a linear array of DNA triple crossover tiles to controllably template the self-assembly of single-layer or double-layer linear arrays of streptavidin molecules and streptavidin-conjugated nanogold particles through biotin-streptavidin interaction. The organization of streptavidin and its conjugated gold nanoparticles into periodic arrays was visualized by atomic force microscopy and scanning electron microscopy.
authors.affiliation: Department of Computer Science, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Hanying
authors.initials: H
authors.lastname: Li
authors.forename: Sung Ha
authors.initials: SH
authors.lastname: Park
authors.forename: John H
authors.initials: JH
authors.lastname: Reif
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010316:Particle Size; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D001710:Biotin; D006046:Gold; D004247:DNA; D019809:Streptavidin