[Cloning and sequence analysis of Chlamydia trachomatis heat shock protein 10].
OBJECTIVE
To obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.


METHODS
cHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.


RESULTS
cHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.


CONCLUSIONS
cHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.
Zhang
Hong-Ye
HY
Clinical Laboratory, Nanjing Yuhua Hospital, Nanjing, Jiangsu 210012, China.
Lu
Jin-Chun
JC
D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018835:Chaperonin 10 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000276:immunology; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018835:Chaperonin 10