title: Suppressive silver methods--a tool for identifying axotomy-induced neuron degeneration.
abstract: Suppressive silver methods evolved from empirical observations about 50 years ago that argyrophilia of normal nerve fibers can be suppressed by a short period of oxidation of tissue sections, whereas degenerating nerve fibers in the same preparations were still clearly visible. Based on this property, suppressive silver impregnation became the main technique for investigating pathways in the central nervous system until the early 1970s. Suppressive silver methods were also found to visualize degenerating nerve cell bodies, in addition to degenerating nerve fibers. This possibility has given these methods an important place among current tools for identifying neuronal degeneration in trauma, disease and toxicity. In this article we demonstrate and review the usefulness of suppressive silver methods in identifying neurons undergoing degeneration as a result of peripheral or central axon injury in immature animals. The documentation is based on previously published data from experiments in which silver impregnation was used to demonstrate degeneration of motoneurons following pure motor axon injury or mixed peripheral nerve injury, as well as on new results on degeneration-induced argyrophilia in the inferior olive following cerebellar lesions. We find that silver precipitates resulting from these injuries are localized either to the entire neuronal cytoplasm, to a few (typically two) intranuclear bodies, or to both sites. The findings are discussed in relation to morphological features of apoptosis, necrosis and retrograde neuronal responses. We suggest that suppressive silver methods allow visualization of different processes of neuronal degeneration, and therefore may be a useful adjunct for identifying axotomy-induced neuronal degeneration.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Retzius väg 8, B2:5, SE-17177 Stockholm, Sweden. Gunnar.Grant@neuro.ki.se
authors.forename: Gunnar
authors.initials: G
authors.lastname: Grant
authors.forename: H
authors.initials: H
authors.lastname: Holländer
authors.forename: H
authors.initials: H
authors.lastname: Aldskogius
mesh_terms: D000818:Animals; D019771:Axotomy / Q000379:methods*; D006801:Humans; D009410:Nerve Degeneration / Q000473:pathology*; D009474:Neurons / Q000737:chemistry / Q000473:pathology*; D016622:Silver Staining / Q000379:methods*