Expression and characterization of recombinant NH2-terminal cell binding fragment of vitronectin in E. coli.
A recombinant peptide fragment of vitronectin (rVN143), that includes the Arg-Gly-Asp (RGD) cell recognition site, was expressed in Escherichia coli using a prokaryotic expression system. The addition of recombinant rVN143 peptide enhances cell adhesion and proliferation similar (approximately 70%) to those of native VN.
Jang
Jun-Hyeog
JH
Intellectual Biointerface Engineering Center, Seoul National University, College of Dentistry, Seoul 110-768, Korea.
Koak
Jai-Young
JY
Kim
Sun-Chul
SC
Hwang
Jung-Hee
JH
Lee
Jai-Bong
JB
Jang
Ik-Tae
IT
Chung
Chong-Pyoung
CP
Heo
Seong-Joo
SJ
D001665:Binding Sites; D040761:Biomimetic Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D013329:Structure-Activity Relationship; D019096:Vitronectin / Q000096:biosynthesis* / Q000145:classification / Q000494:pharmacology*
D010446:Peptide Fragments; D019096:Vitronectin