title: Inhibition of matrix metalloproteinase-2 activity by siderophores of Pseudomonas species.
abstract: To obtain a novel matrix metalloproteinase (MMP) inhibitor produced by bacteria, we have focused on the chelating activity of siderophores. Several siderophore-producing bacteria were isolated from soil using chrome azurol S agar plates and then the effect of siderophores on MMP-2 activity was assayed by gelatin zymography. The results showed that partially purified siderophores from ten isolated strains inhibited MMP-2 activity. Among these strains, two were non-fluorescent and eight were fluorescent Pseudomonas species. From these eight strains, pyoverdine-type siderophores were detected. The Zn(2+)-chelating activity of these siderophores correlated with the inhibition of MMP-2 activity. Therefore, it is considered that siderophores such as pyoverdines inhibit MMP-2 activity by chelating Zn(2+) on the active site of MMP-2.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, 305-8572 Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Akutsu-Shigeno
authors.forename: T
authors.initials: T
authors.lastname: Nakajima-Kambe
authors.forename: S
authors.initials: S
authors.lastname: Inomata
authors.forename: N
authors.initials: N
authors.lastname: Nomura
authors.forename: T
authors.initials: T
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Uchiyama
mesh_terms: D001665:Binding Sites; D002853:Chromatography, Liquid; D062385:Hydroxybenzoates; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D015032:Zinc / Q000737:chemistry
chemical_list: D062385:Hydroxybenzoates; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides; D017262:Siderophores; C015076:chrome azurol S; C042453:pyoverdin; D020778:Matrix Metalloproteinase 2; D015032:Zinc