title: Prostasomes as zinc ligands in human seminal plasma.
abstract: Prostasomes are small vesicles, containing zinc, secreted by prostate in human seminal plasma and showing a physiological role on sperm properties. In this study, the possible correspondence between prostasomes and a prostatic high molecular weight protein complex, recently indicated as zinc ligand, has been investigated. Isolated prostasomes, examined by scanning electron microscopy, were dialysed to evaluate their zinc binding capacity. Furthermore, seminal plasma Sephadex G-75 elution was carried out before and after prostasome removal. Prostasome preparations, containing typical vesicles of 50-500 nm, showed a positive correlation between their zinc and protein levels. They were able to take up zinc against gradient. Furthermore, the seminal zinc amount, bound to the high molecular weight proteins, was strongly reduced in the free-prostasome sample with respect to the total seminal plasma. This study suggested the correspondence between the prostasomes and a high-sized zinc ligand complex of prostatic origin. Therefore, it demonstrated, for the first time, the zinc binding capacity of prostasomes, a new property which could be related to their biological functions.
authors.affiliation: Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rende, Cosenza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vivacqua
authors.forename: L
authors.initials: L
authors.lastname: Siciliano
authors.forename: M
authors.initials: M
authors.lastname: Sabato
authors.forename: A
authors.initials: A
authors.lastname: Palma
authors.forename: A
authors.initials: A
authors.lastname: Carpino
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008297:Male; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011467:Prostate / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008024:Ligands; C017800:zinc-binding protein; D015032:Zinc