title: Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.
abstract: Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.
authors.affiliation: Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Hong
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Fischer
authors.forename: Ayush
authors.initials: A
authors.lastname: Verma
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Goodman
authors.forename: Todd
authors.initials: T
authors.lastname: Emrick
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D005227:Fatty Acids / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D011487:Protein Conformation; D018036:Selenium Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D019187:Cadmium Compounds; D005227:Fatty Acids; D018036:Selenium Compounds; C016173:undecanoic acid; D011092:Polyethylene Glycols; C058667:cadmium selenide; D002918:Chymotrypsin; C117608:alpha-chymotrypsin