[Polymorphism analysis of Haemophilus influenzae strains isolated from healthy children with respect to amplification reaction].
The random-amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints for 44 isolates H. influenzae obtained from healthy children. Problems with reproducibility and discriminatory power, frequently cited in the literature, were overcome by optimization procedure allowing to achieve reliable conditions for H. influenzae analysed. Particular parameters of RAPD fingerprinting were evaluated with respect to selection of best working primer, DNA polymerase and DNA concentration for amplification pattern. This study proved high sensitivity and efficiency of optimized RAPD profiling applicable for searching the epidemiology traces.
Nowaczek
Aneta
A
Zakład Badania Surowic i Szczepionek PZH w Warszawie.
Augustynowicz
Ewa
E
Slusarczyk
Janusz
J
D002675:Child, Preschool; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D011044:Poland; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity