Comparative activities of cecropin A, melittin, and cecropin A-melittin peptide CA(1-7)M(2-9)NH2 against multidrug-resistant nosocomial isolates of Acinetobacter baumannii.
The in vitro activity of three polycationic peptides, cecropin A, melittin, and cecropin A-melittin hybrid peptide CA(1-7)M(2-9)NH2, alone and in combination with various clinically used antimicrobial agents, was investigated against 32 nosocomial isolates of Acinetobacter baumannii. Antimicrobial activities were measured by MIC, MBC and bacterial killing assay. The peptides demonstrated different ranges of inhibitory values: overall, the organisms were more susceptible to CA(1-7)M(2-9)NH2 (MIC range, 0.25-16 mg/l) than to cecropin A (0.50-32 mg/l) and melittin (0.50-32 mg/l). Synergy was observed when CA(1-7)M(2-9)NH2 and melittin were combined with beta-lactam antibiotics.
Giacometti
Andrea
A
Institute of Infectious Diseases and Public Health, University of Ancona, c/o Ospedale Regionale, via Conca, I-60020 Ancona, Italy. anconacmi@interfree.it
Cirioni
Oscar
O
Kamysz
Wojciech
W
D'Amato
Giuseppina
G
Silvestri
Carmela
C
Del Prete
Maria Simona
MS
Łukasiak
Jerzy
J
Scalise
Giorgio
G
D000150:Acinetobacter / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology*; D018432:Drug Resistance, Multiple; D008555:Melitten / Q000737:chemistry / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C435762:cecropin A(1-7)melittin(2-9); D008555:Melitten; C031161:cecropin A