Automated procedure for improving the RNA isolation step in viral load testing for human immunodeficiency virus.
An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation procedure.
Xu
Min
M
Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA.
Chan
Yung
Y
Fischer
Steven H
SH
Remaley
Alan T
AT
D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification*; D019562:Viral Load
D012367:RNA, Viral