title: Polyelectrolyte-stabilized glucose biosensor based on woodceramics as electrode.
abstract: OBJECTIVE
  Different biosensors for early diagnosis of diabetes have been discussed increasingly. We demonstrate the applicability of a glucose biosensor for rapid and quantitative determination of glucose in experimental sample solutions.
  
  
  METHODS
  Polyethylenimine (PEI)-stabilized glucose oxidase (GOD) was used for the construction of stable glucose biosensor utilizing a simple manufacture procedure. The stabilized glucose oxidase was immobilized by physical adsorption into activated porous and conductive carbonaceous material-woodceramics that served as the immobilization matrix and the electrochemical transducer. The optimum parameters for the construction of the biosensor and the influences of pH and temperature on the biosensor response were explored.
  
  
  RESULTS
  Optimum results were obtained using soaking solutions of 2300 U/ml GOD, 10 mg/ml mediator toluidine blue O (TBO), and 2.0%, w/v PEI. The sensitivity of the resulting biosensor for glucose was 1.22 microA/mM, with a linear response in the concentration range of 0.5-7 mM. The sensor-to-sensor reproducibility was good, the relative standard deviation (R.S.D.) being less than 8.0%. The storage and operational stabilities of the glucose biosensor were good.
  
  
  CONCLUSIONS
  The glucose biosensor provides reproducible and quantitative information on glucose within minutes and is applicable for the detection of glucose in experimental samples.
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ailisuo@hotmail.com
authors.forename: Jun-Min
authors.initials: JM
authors.lastname: Qian
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Suo
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Jin
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D007700:Kinetics; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose