In vivo antigen presentation.
Monoclonal antibodies specific for defined peptide-MHC complexes are now being used to physically detect T-cell receptor ligands. These reagents have resulted in the identification of the cells that present antigen in lymphoid and non-lymphoid tissues after various forms of antigen administration. In addition, recent advances in real-time imaging technology have begun to measure the rate and directionality of T-cell movement relative to antigen-presenting cells in lymph nodes, shedding light on the earliest events in T-cell activation in a physiological setting.
Germain
Ronald N
RN
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Drive, MSC-1892, Bethesda, MD 20892-1892, USA. RGERMAIN@niaid.nih.gov
Jenkins
Marc K
MK
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000032:analysis / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D008856:Microscopy, Fluorescence; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D011948:Receptors, Antigen, T-Cell