title: Intrathecal production of Chlamydia pneumoniae-specific high-affinity antibodies is significantly associated to a subset of multiple sclerosis patients with progressive forms.
abstract: The purpose of this study was to provide further insight into the effective relevance of the association between Chlamydia pneumoniae and MS. We evaluated by ELISA technique cerebrospinal fluid (CSF) and serum levels of anti-C. pneumoniae IgG in 46 relapsing-remitting (RR), 14 secondary progressive (SP) and 11 primary progressive (PP) MS patients grouped according to clinical and Magnetic Resonance Imaging (MRI) evidence of disease activity. Fifty-one patients with other inflammatory neurological disorders (OIND) and 52 with non-inflammatory neurological disorders (NIND) were used as controls. A C. pneumoniae-specific intrathecal IgG synthesis as detected by the relative specific index was present in a small proportion of MS (17%), OIND (22%) and NIND (2%) patients and was significantly more frequent in MS and in OIND than in NIND (p<0.001) and in SP and PP MS than in RR MS patients (p<0.02). Among the patients with C. pneumoniae-specific intratecally produced antibodies, CSF high-affinity anti-C. pneumoniae IgG were found in the majority of SP or PP MS, occasionally in OIND, but not in RR MS and NIND patients. These findings confirm that the presence of a humoral immune response to C. pneumoniae within the central nervous system (CNS) is not selectively restricted to MS, but is shared by several inflammatory neurological conditions. In addition, our results suggest that an intrathecal production of C. pneumoniae-specific high-affinity IgG can occur in a subset of patients with MS progressive forms in which a C. pneumoniae brain chronic persistent infection may play an important pathogenetic role.
authors.affiliation: Multiple Sclerosis Center, Department of Neurology, University of Ferrara, Arcispedale S. Anna, Corso della Giovecca 203, I-44100, Ferrara, Italy. henryfai@tin.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Fainardi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Castellazzi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Casetta
authors.forename: Rosario
authors.initials: R
authors.lastname: Cultrera
authors.forename: Luca
authors.initials: L
authors.lastname: Vaghi
authors.forename: Enrico
authors.initials: E
authors.lastname: Granieri
authors.forename: Carlo
authors.initials: C
authors.lastname: Contini
mesh_terms: D000328:Adult; D000917:Antibody Formation / Q000276:immunology; D001921:Brain / Q000000981:diagnostic imaging / Q000276:immunology / Q000382:microbiology; D002690:Chlamydia Infections / Q000134:cerebrospinal fluid* / Q000150:complications* / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000472:pathogenicity*; D004660:Encephalitis / Q000134:cerebrospinal fluid / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000382:microbiology*; D011859:Radiography
chemical_list: D007074:Immunoglobulin G