title: [Using chamber technique for studying the permeability of dexamethasone sodium phosphate liposome through rabbit colon mucosa in vitro].
abstract: OBJECTIVE
  To investigate the features of liposome-mediated drug permeability through the colon mucosa in vitro.
  
  
  METHODS
  Dexamethasone sodium phosphate liposome (DSP) was prepared using orthogonal design. With in vitro Ussing chamber experiment, the accumulated amount of DSP permeated through rabbit colon mucosa into the receptor chamber and the distribution amount in the colon were determined at different time points during the experiment.
  
  
  RESULTS
  Compared with the prepared DSP solution, DSP liposome decreased the permeated amount of DSP through rabbit colon mucosa. The apparent permeability coefficient (Papp, x10(-6), cm/s1) of the DSP was 31.95+/-7.65, significantly lower than that of DSP solution (88.61+/-18.61). On the other hand, the distribution amount of DSP liposome in the colon mucosa was significantly higher than that of the DSP solution both at 120 and 300 min.
  
  
  CONCLUSIONS
  DSP liposome dosage form may induce less toxicity with better therapeutic effect in the treatment of colitis, which is worthy of further exploitation.
authors.affiliation: Department of Pharmaceutics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. lgfnf@fimmu.com
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
authors.forename: Jian-hai
authors.initials: JH
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Dan
authors.initials: D
authors.lastname: Guo
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: Wang
authors.forename: Lian-bin
authors.initials: LB
authors.lastname: Hou
mesh_terms: D000818:Animals; D003106:Colon / Q000378:metabolism*; D003907:Dexamethasone / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004337:Drug Carriers; D007413:Intestinal Mucosa / Q000378:metabolism*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D010316:Particle Size; D010539:Permeability; D011817:Rabbits
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D003907:Dexamethasone