title: Spatial comparison between wall shear stress measures and porcine arterial endothelial permeability.
abstract: A better understanding of how hemodynamic factors affect the integrity and function of the vascular endothelium is necessary to appreciate more fully how atherosclerosis is initiated and promoted. A novel technique is presented to assess the relation between fluid dynamic variables and the permeability of the endothelium to macromolecules. Fully anesthetized, domestic swine were intravenously injected with the albumin marker Evans blue dye, which was allowed to circulate for 90 min. After the animals were euthanized, silicone casts were made of the abdominal aorta and its iliac branches. Pulsatile flow calculations were subsequently made in computational regions derived from the casts. The distribution of the calculated time-dependent wall shear stress in the external iliac branches was directly compared on a point-by-point basis with the spatially varying in vivo uptake of Evans blue dye in the same arteries. The results indicate that in vivo endothelial permeability to albumin decreases with increasing time-average shear stress over the normal range. Additionally, endothelial permeability increases slightly with oscillatory shear index.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Himburg
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Grzybowski
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Hazel
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: LaMack
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Li
authors.forename: Morton H
authors.initials: MH
authors.lastname: Friedman
mesh_terms: D000418:Albumins / Q000493:pharmacokinetics; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism* / Q000502:physiology; D002199:Capillary Permeability; D016319:Corrosion Casting; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology; D005070:Evans Blue; D005260:Female; D007083:Iliac Artery / Q000378:metabolism* / Q000502:physiology; D046911:Macromolecular Substances; D008955:Models, Cardiovascular; D011673:Pulsatile Flow; D013314:Stress, Mechanical; D013552:Swine; D013997:Time Factors
chemical_list: D000418:Albumins; D046911:Macromolecular Substances; D005070:Evans Blue