title: High resolution HLA-DRB1 identification of a Caucasian population.
abstract: Polymerase chain reaction-sequence-specific oligonucleotide probes typing methods have been applied to 1000 individuals from the Northern Ireland population to give human leukocyte antigen DRB1 (HLA-DRB1) allele assignment. HLA-DRB1 allele frequencies and four-locus haplotypes (A/B/C/DR) for this Caucasian population, based on HLA class I and class II allele assignment, are now presented. No significant deviations from Hardy-Weinberg proportions were observed. The HLA-C locus exhibited marginal evidence of selection (p<0.03, uncorrected one-sided test) in the direction of balancing selection; the HLA-A, -B, and -DRB1 allele frequency distributions were compatible with expectations under a neutral model (which does not mean that selection is not operating). Evidence for selection was seen on haplotypes HLA-A*010101-B*0801-DRB1*030101 and HLA-A*290201-B*440301-DRB1*070101 based on their patterns of linkage disequilibrium.
authors.affiliation: Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland. fionnuala.williams@bll.n-i.nhs.uk
authors.forename: Fionnuala
authors.initials: F
authors.lastname: Williams
authors.forename: Ashley
authors.initials: A
authors.lastname: Meenagh
authors.forename: Rich
authors.initials: R
authors.lastname: Single
authors.forename: Mark
authors.initials: M
authors.lastname: McNally
authors.forename: Philip
authors.initials: P
authors.lastname: Kelly
authors.forename: Mark P
authors.initials: MP
authors.lastname: Nelson
authors.forename: Diogo
authors.initials: D
authors.lastname: Meyer
authors.forename: Alex
authors.initials: A
authors.lastname: Lancaster
authors.forename: Glenys
authors.initials: G
authors.lastname: Thomson
authors.forename: Derek
authors.initials: D
authors.lastname: Middleton
mesh_terms: D000483:Alleles; D003062:Codon / Q000235:genetics; D004739:England / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D015810:Linkage Disequilibrium; D008163:Luminescent Measurements; D008957:Models, Genetic; D009660:Northern Ireland; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012606:Scotland / Q000208:ethnology; D044465:White People / Q000235:genetics*
chemical_list: D003062:Codon; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015345:Oligonucleotide Probes