A general strategy to convert the MerR family proteins into highly sensitive and selective fluorescent biosensors for metal ions.
The MerR family metal-regulatory proteins are converted into fluorescent biosensors that can detect different metal ions with high sensitivity and selectivity.
Chen
Peng
P
Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, Illinois 60637, USA.
He
Chuan
C
D001426:Bacterial Proteins / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D002412:Cations; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; C051124:MerR protein, Bacteria; D008670:Metals; D004247:DNA