Studies of biological functions in blood cells from individuals with large platelets.
The present study was performed to explore differences in the expression of P-selectin and IL-8 production of blood cells in healthy individuals with large size platelets (MI-families) as compared to people having normal size platelets. A positive correlation between LPS-induced IL-8 production per platelet in whole blood and mean platelet volume (MPV) was found in the large platelet group (R=0.74, P<0.05). When the large and normal groups were combined the correlation was nearly, but not quite significant (R=0.46, P<0.06). There was also a positive correlation between sP-selectin and MPV (R=0.42, P<0.05). Furthermore, IL-8 in serum was positively correlated to sP-selectin in serum (R=0.68, P<0.005). sP-selectin baseline values in citrated plasma correlated significantly with values found in serum (R=0.72, P<0.0005), indicating that sP-selectin in blood originates from the platelets rather than from endothelial cells. Significant correlations were also found in both groups between P-selectin and CD40L (R=0.44, P<0.05) and P-selectin and RANTES (R=0.44, P<0.05). A significant correlation was also found between PDGF and RANTES (R=0.44, P<0.05). Our results suggest that larger platelets enhance the production of IL-8 more than normally sized platelets. This phenomenon is probably mediated through P-selectin exposed on platelets.
Breimo
Einar S
ES
Department of Biochemistry, Institute of Medical Biology, University of Tromsø, Norway. einarbr@fagmed.uti.no
Østerud
Bjarne
B
D000328:Adult; D001772:Blood Cell Count; D001773:Blood Cells / Q000187:drug effects / Q000502:physiology*; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D023201:CD40 Ligand / Q000097:blood; D048429:Cell Size / Q000502:physiology; D018946:Chemokine CCL5 / Q000097:blood; D003094:Collagen / Q000494:pharmacology; D003627:Data Interpretation, Statistical; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D019007:P-Selectin / Q000097:blood*; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D018946:Chemokine CCL5; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D019007:P-Selectin; D010982:Platelet-Derived Growth Factor; D014409:Tumor Necrosis Factor-alpha; C433788:lipopolysaccharide, E. coli O26-B6; C075278:platelet-derived growth factor A; D023201:CD40 Ligand; D003094:Collagen; D004837:Epinephrine