Transdermal iontophoresis of 5-fluorouracil combined with electroporation and laser treatment.
The influence of iontophoresis and other physical enhancement methods such as electroporation and erbium:yttrium-aluminum-garnet (YAG) laser on the skin permeation of 5-fluorouracil (5-FU) was examined. Iontophoresis increased the in vitro transdermal transport of both the anionic and non-ionic forms of 5-FU. A combination of electroporation pretreatment and subsequent iontophoresis resulted in a higher permeation of 5-FU than either technique alone. It appeared that electroporation treatment exerted a disruptive influence on the stratum corneum (SC). The SC layers in the skin were partly ablated by the laser, resulting in a great enhancement effect on the skin permeation of 5-FU. Application of iontophoresis further increased the drug permeation across laser-pretreated skin. The laser was consistently the most potent technique to enhance 5-FU delivery among the physical enhancement methods examined in this study.
Fang
Jia-You
JY
Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan. fajy@mail.cgu.edu.tw
Hung
Chi-Feng
CF
Fang
Yi-Ping
YP
Chan
Te-Fu
TF
D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage*; D066298:In Vitro Techniques; D007478:Iontophoresis; D007834:Lasers; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D012869:Skin Absorption; D013997:Time Factors
D005472:Fluorouracil