title: Protein profile of symbiotic bacteria Mesorhizobium loti MAFF303099 in mid-growth phase.
abstract: Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications.
authors.affiliation: Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. kajiwara@affrc.go.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kajiwara
authors.forename: Takayo
authors.initials: T
authors.lastname: Kaneko
authors.forename: Masami
authors.initials: M
authors.lastname: Ishizaka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kouchi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D000070116:Lotus / Q000382:microbiology; D020560:Proteobacteria / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis / Q000502:physiology
chemical_list: D020543:Proteome