title: Practical bench comparison of BBL CHROMagar Orientation and standard two-plate media for urine cultures.
abstract: A total of 1023 urine samples sent for routine culture were plated onto sheep blood and MacConkey agars and a BBL CHROMagar Orientation (CO; Becton Dickinson, Cockeysville, Md.) plate, and the results were compared. Of these, 250 urine samples (24%) grew >10000 CFU of one or two putative pathogens/ml and 773 showed no growth (NG), mixed growth of <10000 CFU/ml, or three or more strains (mixed). The CO and conventional medium results agreed completely for 595 cultures with NG or <10000 CFU/ml. An additional 178 urine samples yielded clinically insignificant differences. Both medium sets essentially agreed on quantities and identification for 400 single-pathogen cultures and 9 mixed cultures. With the caveat that CO cannot differentiate Klebsiella, Enterobacter, and Serratia spp., enteric pathogens were identified only by morphology and color on CO. Direct visual differentiation of group B streptococci from lactobacilli is not possible, but lactobacillus cells always exhibited easily recognizable morphology on Gram stain. Of 108 paired organism susceptibility results encompassing 2268 drug-pathogen combinations, there were 3% errors and only 1% very major errors. Use of CO allowed a >50% reduction in inoculation time and a >20% reduction in work-up time. For our laboratory, with 50% "no growth" and ca. 25% significant results (50% Escherichia coli), CO allowed time and workup cost savings for a majority of cultures. A cost analysis (time and supplies for our laboratory) showed that if CO is used alone, the break-even level for CO pricing is US dollars 1.78; if CO and blood agar are both used, the break-even pricing for CO is US dollars 1.53.
authors.affiliation: Department of Pathology. Department of Medicine, Stanford University Medical School, Stanford, California 94305, USA.
authors.forename: Holly A
authors.initials: HA
authors.lastname: D'Souza
authors.forename: Mary
authors.initials: M
authors.lastname: Campbell
authors.forename: Ellen Jo
authors.initials: EJ
authors.lastname: Baron
mesh_terms: D000362:Agar; D001419:Bacteria / Q000254:growth & development*; D001437:Bacteriuria / Q000175:diagnosis / Q000382:microbiology*; D003365:Costs and Cost Analysis; D003470:Culture Media; D006801:Humans
chemical_list: D003470:Culture Media; D000362:Agar