title: Serodiagnosis of chronic and acute Chagas' disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries.
abstract: An enzyme-linked immunosorbent assay to diagnose Chagas' disease by a serological test was performed with Trypanosoma cruzi recombinant antigens (JL8, MAP, and TcPo). High sensitivity (99.4%) and specificity (99.3%) were obtained when JL8 was combined with MAP (JM) and tested with 150 serum samples from chagasic and 142 nonchagasic individuals. Moreover, JM also diagnosed 84.2% of patients in the acute phase of T. cruzi infection.
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, UNIFESP, São Paulo, Brazil. eumezawa@usp.br
authors.forename: Eufrosina S
authors.initials: ES
authors.lastname: Umezawa
authors.forename: Alejandro O
authors.initials: AO
authors.lastname: Luquetti
authors.forename: Gabriela
authors.initials: G
authors.lastname: Levitus
authors.forename: Carlos
authors.initials: C
authors.lastname: Ponce
authors.forename: Elisa
authors.initials: E
authors.lastname: Ponce
authors.forename: Diana
authors.initials: D
authors.lastname: Henriquez
authors.forename: Susana
authors.initials: S
authors.lastname: Revollo
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
authors.forename: Octavio
authors.initials: O
authors.lastname: Sousa
authors.forename: Baldip
authors.initials: B
authors.lastname: Khan
authors.forename: José Franco
authors.initials: JF
authors.lastname: da Silveira
mesh_terms: D000208:Acute Disease; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000175:diagnosis*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D011994:Recombinant Proteins