title: Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue.
abstract: A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated.
authors.affiliation: Department of Psychological Medicine, Institute of Psychiatry, UK. k.pennington@iop.kcl.ac.uk
authors.forename: Kyla
authors.initials: K
authors.lastname: Pennington
authors.forename: Emma
authors.initials: E
authors.lastname: McGregor
authors.forename: Clare L
authors.initials: CL
authors.lastname: Beasley
authors.forename: Ian
authors.initials: I
authors.lastname: Everall
authors.forename: David
authors.initials: D
authors.lastname: Cotter
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dunn
mesh_terms: D001923:Brain Chemistry; D004229:Dithiothreitol; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007525:Isoelectric Focusing; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins; D004229:Dithiothreitol