Peptidomics of the locust corpora allata: identification of novel pyrokinins (-FXPRLamides).
The peptidomes of the corpora allata of Locusta migratoria and Schistocerca gregaria were investigated by both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (nanoLC-Q-TOF MSMS). The pyrokinin (-FXPRLamide) family seems to be predominant. In addition to the known pyrokinins, we de novo sequenced four pyrokinins in L. migratoria and five in S. gregaria. In addition, one pyrokinin-like peptide (-PRLamide) was identified in S. gregaria. Besides the -(FX)PRLamides, FLRFamide-1, the allatostatins (A family) and numerous as yet unidentified peptides are also present in the corpora allata.
Clynen
E
E
Laboratory for Developmental Physiology, Genomics and Proteomics, K.U. Leuven, 3000, Leuven, Belgium. Elke.Clynen@bio.kuleuven.ac.be
Baggerman
G
G
Huybrechts
J
J
Vanden Bosch
L
L
De Loof
A
A
Schoofs
L
L
D000595:Amino Acid Sequence; D000818:Animals; D003335:Corpora Allata / Q000737:chemistry*; D006110:Grasshoppers / Q000737:chemistry*; D007301:Insect Hormones / Q000032:analysis / Q000737:chemistry; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis* / Q000737:chemistry; D010449:Peptide Mapping; D040901:Proteomics; D012016:Reference Values; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; C079229:pyrokinin