A cyclo peptide activates signaling events and promotes growth and the production of the bone matrix.
The interaction of bone cells and their underlying extracellular matrix impacts biological processes such as maintenance of tissue integrity. The biological recognition of the extracellular matrix by attached cells is mediated by the activity of integrins that recognize adhesive-specific domains. The most widely recognized adhesive motif is the RGD sequence, common to many of the adhesive matrix molecules. Here, we show that cyclo DFKRG which was previously selected to increase cell adhesion of human bone marrow stromal cells (HBMSC), increases both cell differentiation and mineralization through activation of tyrosine kinases, focal adhesion kinase (p(125)FAK) and Mitogen Activated Protein (MAP) kinases.
Pallu
S
S
INSERM U.577, Université Bordeaux Victor Segalen, Bordeaux 2, 33076 Bordeaux Cedex, France.
Bareille
R
R
Dard
M
M
Kessler
H
H
Jonczyk
A
A
Vernizeau
M
M
Amédée-Vilamitjana
J
J
D001857:Bone Matrix / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002127:Calcium Isotopes; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
D002127:Calcium Isotopes; D010456:Peptides, Cyclic; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases