title: Arf GAPs: multifunctional proteins that regulate membrane traffic and actin remodelling.
abstract: The ADP-ribosylation factor (Arf) Arf GTPase-activating proteins (GAPs) are a family of proteins that induce hydrolysis of GTP bound to Arf. A conserved domain containing a zinc finger motif mediates catalysis. The substrate, Arf.GTP, affects membrane trafficking and actin remodelling. Consistent with activity as an Arf regulator, the Arf GAPs affect both of these pathways. However, the Arf GAPs are likely to have Arf-independent activities that contribute to their cellular functions. Structures of the Arf GAPs are diverse containing catalytic, protein-protein interaction and lipid interaction domains in addition to the Arf GAP domain. Some Arf GAPs have been identified and characterized on the basis of activities other than Arf GAP. Here, we describe the Arf GAP family, enzymology of some members of the Arf GAP family and known functions of the proteins. The results discussed illustrate roles for both Arf-dependent and -independent activities in the regulation of cellular architecture.
authors.affiliation: Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building. 37 Room 4118, Bethesda, MD 20892, USA. randazzo@helix.nih.gov
authors.forename: Paul A
authors.initials: PA
authors.lastname: Randazzo
authors.forename: Dianne S
authors.initials: DS
authors.lastname: Hirsch
mesh_terms: D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000199:Actins; D020690:GTPase-Activating Proteins; D020727:ADP-Ribosylation Factors