Antioxidative responses of tobacco expressing a bacterial glutathione reductase.
Reports on stress response of tobacco expressing a bacterial glutathione reductase (GR) do not agree. To clarify this situation we investigated several parameters using the tobacco BelW3 line and its transformant BelW3gor expressing an E. coli GR. This alteration in the activity of GR led to an ambiguous modification of the antioxidative system. In contrast to the wild type, the transgenic tobacco suffered lipid peroxidation under moderate light intensities, while it was found to be more resistant towards oxidative stress induced by paraquat or hydrogen peroxide. Transcript levels for violaxanthin deepoxidase and cytosolic Cu-Zn-superoxide dismutase were strongly reduced in BelW3gor plants as compared to BelW3.
Lederer
Barbara
B
Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-78457 Konstanz, Germany.
Böger
Peter
P
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005980:Glutathione Reductase / Q000235:genetics* / Q000378:metabolism; D008027:Light; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D000975:Antioxidants; D012333:RNA, Messenger; D018749:RNA, Plant; D002374:Catalase; D005980:Glutathione Reductase