[Luminous plant and animals or the expression of aequorin and "chameleon" probes: a new light in calcium signaling].
Calcium ion is a universal second messenger in numerous cell physiological processes. The paper describes the structure and the activation mechanisms of the bioluminescent (aequorin) and fluorescent based GFP calcium sensitive probes (Cameleon) and the data obtained with such probes in genetically transformed animal and vegetal organisms. The importance of these in vivo Ca2+ imaging molecules in the understanding of calcium signalling is discussed.
Thuleau
Patrice
P
UMR CNRS-UPS 5546, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, F-31326 Castanet-Tolosan.
Leclerc
Catherine
C
Xiong
Tou-Cheu
TC
Mazars
Christian
C
Leclerc
Catherine
C
Moreau
Marc
M
D000331:Aequorin / Q000378:metabolism*; D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000378:metabolism
D008164:Luminescent Proteins; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D000331:Aequorin