A Method to Identify p62's UBA Domain Interacting Proteins.
The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective. Using the rabbit reticulocyte lysate in vitro expression cloning system, we have successfully identified eleven proteins that interact with p62's UBA domain, and the majority of the eleven proteins are associated with neurodegenerative disorders, such as Alzheimer's disease. Therefore, p62 may play a novel regulatory role through its UBA domain. Our approach provides an easy route to the characterization of UBA domain interacting proteins and its application will unfold the important roles that the UBA domain plays.
Pridgeon
Julia W.
JW
Department of Biological Sciences, Program in Cellular and Molecular Biosciences. 331 Funchess Hall, Auburn University, Auburn, AL 36849. USA.
Geetha
Thangiah
T
Wooten
Marie W.
MW