Protective efficacy of hepatitis E virus DNA vaccine administered by gene gun in the cynomolgus macaque model of infection.
The protective efficacy of a DNA vaccine against hepatitis E virus (HEV) infection was tested in cynomolgus macaques (cynos) vaccinated with a plasmid containing a full-length HEV open-reading frame 2 (ORF2) sequence (Burmese strain) and subsequently challenged with a heterologous strain of HEV (Mexican strain). Cynos administered vaccine by gene gun developed antibodies to HEV (anti-HEV), whereas cynos administered vaccine by intradermal injections and cynos administered a mock DNA construct did not develop anti-HEV. Anti-HEV-positive cynos were protected from HEV infection after challenge with an inoculum that produced infection in the anti-HEV-negative cynos. These results indicate that DNA vaccine with HEV ORF2 administered by gene gun is protective against a heterologous viral challenge.
Kamili
Saleem
S
Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30084, USA.
Spelbring
John
J
Carson
Dorrie
D
Krawczynski
Krzysztof
K
D000818:Animals; D019470:Biolistics / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood; D016751:Hepatitis E / Q000517:prevention & control*; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology*; D008251:Macaca; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
D006508:Hepatitis Antibodies; D019444:Vaccines, DNA; D014761:Viral Hepatitis Vaccines