Neutrophil and monocyte adhesion molecules in bronchopulmonary dysplasia, and effects of corticosteroids.
OBJECTIVE
To study a longitudinal change in the expression of adhesion molecules CD11b, CD18, and CD62L on neutrophils and monocytes in very low birth weight babies who develop respiratory distress syndrome, to compare these levels between bronchopulmonary dysplasia (BPD) and non-BPD infants, and to assess the effect of corticosteroid treatment on these adhesion molecules.


METHODS
Of 40 eligible neonates, 11 neonates were oxygen dependent at 36 weeks (BPD 36 weeks), 16 infants were oxygen dependent at 28 days, but not at 36 weeks (BPD d28), and 13 infants did not develop BPD. Seventeen neonates received a six day course of steroid treatment. Expression of CD11b, CD18, and CD62L was measured on neutrophils and monocytes in arterial blood on days 1, 3, 7, 14, 21, and 28, and before and 2-3 days after initiation of dexamethasone treatment by flow cytometry.


RESULTS
CD18 expression on neutrophils and monocytes and CD62L on neutrophils, measured as mean fluorescent intensity, was significantly decreased in BPD neonates compared to non-BPD neonates on days 1-28. Dexamethasone treatment significantly decreased CD11b, CD18, and CD62L expression on neutrophils, and CD11b and CD18L expression on monocytes.


CONCLUSIONS
Decreased CD18 expression on neutrophils and monocytes, and decreased CD62L expression on neutrophils, measured as mean fluorescent intensity during the first four weeks of life in micropremies may be risk factors and early predictors of BPD. Dexamethasone use was associated with decreased expression of CD11b, CD18, and CD62L.
Ballabh
P
P
Division of Neonatology, New York Presbyterian Hospital, Weill Medical College of Cornell University, New York, USA. Pballabh@msn.com
Simm
M
M
Kumari
J
J
Krauss
A N
AN
Jain
A
A
Califano
C
C
Lesser
M L
ML
Cunningham-Rundles
S
S
D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D015703:Antigens, CD / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001997:Bronchopulmonary Dysplasia / Q000097:blood / Q000276:immunology*; D039481:CD11b Antigen / Q000097:blood; D018821:CD18 Antigens / Q000097:blood; D003907:Dexamethasone / Q000627:therapeutic use*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005453:Fluorescence; D006801:Humans; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D019041:L-Selectin / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D015703:Antigens, CD; D015415:Biomarkers; D039481:CD11b Antigen; D018821:CD18 Antigens; D019041:L-Selectin; D003907:Dexamethasone