Amplified fragment length polymorphism fingerprinting is an effective technique to distinguish streptococcus pneumoniae from other Streptococci and an efficient alternative to pulsed-field gel electrophoresis for molecular typing of pneumococci.
Amplified fragment length polymorphism versus pulsed-field gel electrophoresis was used for fingerprinting of 85 macrolide-resistant pneumococcal isolates identified by using primarily phenotypic methods. Confirmation of identification by 16S rRNA sequencing revealed that 27 isolates were actually nonpneumococci. Amplified fragment length polymorphism but not pulsed-field gel electrophoresis offered simultaneous and accurate discrimination between pneumococci and nonpneumococcal species.
Neeleman
Chris
C
Department of Intensive Care, Radboud University Hospital, Nijmegen, The Netherlands.
Klaassen
Corné H W
CH
de Valk
Hanneke A
HA
de Ruiter
Maaike T
MT
Mouton
Johan W
JW
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013291:Streptococcus / Q000145:classification* / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics
D012336:RNA, Ribosomal, 16S