title: Amplified fragment length polymorphism fingerprinting is an effective technique to distinguish streptococcus pneumoniae from other Streptococci and an efficient alternative to pulsed-field gel electrophoresis for molecular typing of pneumococci.
abstract: Amplified fragment length polymorphism versus pulsed-field gel electrophoresis was used for fingerprinting of 85 macrolide-resistant pneumococcal isolates identified by using primarily phenotypic methods. Confirmation of identification by 16S rRNA sequencing revealed that 27 isolates were actually nonpneumococci. Amplified fragment length polymorphism but not pulsed-field gel electrophoresis offered simultaneous and accurate discrimination between pneumococci and nonpneumococcal species.
authors.affiliation: Department of Intensive Care, Radboud University Hospital, Nijmegen, The Netherlands.
authors.forename: Chris
authors.initials: C
authors.lastname: Neeleman
authors.forename: Corné H W
authors.initials: CH
authors.lastname: Klaassen
authors.forename: Hanneke A
authors.initials: HA
authors.lastname: de Valk
authors.forename: Maaike T
authors.initials: MT
authors.lastname: de Ruiter
authors.forename: Johan W
authors.initials: JW
authors.lastname: Mouton
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013291:Streptococcus / Q000145:classification* / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S