A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds.
A spontaneous point mutation in pilQ (pilQ1) resulted in phenotypic suppression of a hemoglobin (Hb) receptor mutant (hpuAB mutant), allowing gonococci to grow on Hb as the sole source of iron. PilQ, formerly designated OMP-MC, is a member of the secretin family of proteins located in the outer membrane and is required for pilus biogenesis. The pilQ1 mutant also showed decreased piliation and transformation efficiency. Insertional inactivation of pilQ1 resulted in the loss of the Hb utilization phenotype and decreased entry of free heme. Despite the ability of the pilQ1 mutant to use Hb for iron acquisition and porphyrin, there was no demonstrable binding of Hb to the cell surface. The pilQ1 mutant was more sensitive to the toxic effect of free heme in growth medium and hypersensitive to the detergent Triton X-100 and multiple antibiotics. Double mutation in pilQ1 and tonB had no effect on these phenotypes, but a double pilQ1 pilT mutant showed a reduction in Hb-dependent growth and decreased sensitivity to heme and various antimicrobial agents. Insertional inactivation of wild-type pilQ also resulted in reduced entry of heme, Triton X-100, and some antibiotics. These results show that PilQ forms a channel that allows entry of heme and certain antimicrobial compounds and that a gain-of function point mutation in pilQ results in TonB-independent, PilT-dependent increase of entry.
Chen
Ching-ju
CJ
Department of Medicine. Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7031, USA. chencj@med.unc.edu
Tobiason
Deborah M
DM
Thomas
Christopher E
CE
Shafer
William M
WM
Seifert
H Steven
HS
Sparling
P Frederick
PF
D000251:Adenosine Triphosphatases / Q000502:physiology; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D001426:Bacterial Proteins / Q000502:physiology; D036781:Fimbriae Proteins / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000502:physiology; D006418:Heme / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology; D020409:Molecular Motor Proteins / Q000502:physiology; D009344:Neisseria gonorrhoeae / Q000737:chemistry*; D010641:Phenotype; D017354:Point Mutation; D021381:Protein Transport; D014169:Transformation, Bacterial
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D008565:Membrane Proteins; D020409:Molecular Motor Proteins; C084295:pilQ protein, bacteria; C057616:tonB protein, Bacteria; D036781:Fimbriae Proteins; D006418:Heme; D000251:Adenosine Triphosphatases