Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: a high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract.
A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of approximately 50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.
Myung
Sunnie
S
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Lee
Young Jin
YJ
Moon
Myeong Hee
MH
Taraszka
John
J
Sowell
Rena
R
Koeniger
Stormy
S
Hilderbrand
Amy E
AE
Valentine
Stephen J
SJ
Cherbas
Lucy
L
Cherbas
Peter
P
Kaufmann
Thomas C
TC
Miller
David F
DF
Mechref
Yehia
Y
Novotny
Milos V
MV
Ewing
Michael A
MA
Sporleder
C Ray
CR
Clemmer
David E
DE
D000818:Animals; D029721:Drosophila Proteins / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D029721:Drosophila Proteins; D010455:Peptides