[Basic study of biodegradable controlled release chemotherapy].
This study sought to assess the biocompatibility of P(DA-SA)-Adriamycin, a new controlled-release chemotherapy system, in rabbit brain, and to examine its controlled release effect both in vitro and in vivo and its curative effects in vitro. The reaction of animal brain to the implanted P(DA-SA) or P(DA-SA)-Adriamycin was observed. The controlled-release profiles in phosphate buffer solutions and in rabbit brain were measured by UV spectrometry. Then, through flow cytometer, the rate of apoptosis in cultured glioma cells was tested. The reaction of rabbit brain to P(DA-SA) polymer was moderate and not significantly different from that to Gelfoam. The controlled-release rate of P(DA-SA)-Adriamycin in vitro and in vivo was stable and the duration of controlled-release of P(DA-SA)-Adriamycin spanned three weeks. The rate for apoptosis of glioma cells of P(DA-SA)-Adriamycin group was 69.9%, which was significantly higher than that of the control group. In conclusion, P (DA-SA)-Adriamycin controlled release chemotherapy system that bears curative effect has favorable controlled-release effect and good biocompatibility in rabbit brain. This system has potential value in treatment of malignant brain tumor.
Zhang
Tao
T
Department of Neurosurgery, Tongji Hospitol, Huazhong University of Science and Technology, Wuhan 430030.
Zhang
Zhiqiang
Z
Liu
Zonglin
Z
Liu
Shixin
S
Zhang
Yihu
Y
Zhou
Zhibin
Z
Lei
Ting
T
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D003652:Decanoic Acids / Q000378:metabolism; D004317:Doxorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000378:metabolism; D004343:Drug Implants; D008422:Materials Testing; D011091:Polyesters / Q000378:metabolism; D011817:Rabbits; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D003652:Decanoic Acids; D004337:Drug Carriers; D004343:Drug Implants; D011091:Polyesters; D004317:Doxorubicin