Techniques for studying the oxygen-sensitive transcription factor FNR from Escherichia coli.
A large variety of techniques can be adapted for use with oxygen-sensitive samples. The growth of cells and in vivo analyses, as well as protein purification and in vitro assays, can be executed either by performing necessary steps in anaerobic environments (ranging from simple closed containers to the anaerobic chamber) or by circumventing the need for anaerobiosis with the use of oxygen-resistant protein variants.
Sutton
Victoria R
VR
Department of Biomolecular Chemistry, University of Wisconsin, 574 Medical Science Center, 1300 University Avenue, Madison, Wisconsin 53706, USA.
Kiley
Patricia J
PJ
D001671:Biochemistry / Q000379:methods*; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D010100:Oxygen / Q000378:metabolism*
D003470:Culture Media; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D010100:Oxygen