[Genotype of human calicivirus from infants with diarrhea in Guangzhou city in 2001].
OBJECTIVE
To understand the characteristics of human calicivirus (HuCV) infection in infants with diarrhea in Guangzhou city and to study genotype of the virus.


METHODS
The authors collected fecal specimens from 22 children with acute nonbacterial gastroenteritis from November to December, 2001. HuCV was detected from the specimens by RT-PCR. The PCR products were cloned into the PMD18-T cloning vector and sequenced.


RESULTS
HCV was detected from the specimens of 2 cases (9%, 2/22). The nucleotide sequence analysis revealed that the virus strains belonged to genotype 2 of Norwalk-like viruses.


CONCLUSIONS
HuCV is one of the pathogens causing diarrhea in infants and young children in Guangzhou area. HuCV infection occurred sporadically in autumn and winter.
Xie
Jian-ping
JP
Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China.
Fang
Zhao-yin
ZY
Gong
Si-tang
ST
Zhu
Bing
B
Ou
Wen-ji
WJ
Wan
Gen-ping
GP
Lai
Gui-xiang
GX
He
Cui-juan
CJ
D001483:Base Sequence; D002139:Caliciviridae / Q000235:genetics*; D017250:Caliciviridae Infections / Q000150:complications* / Q000821:virology; D002681:China; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003968:Diarrhea, Infantile / Q000209:etiology*; D004403:Dysentery / Q000209:etiology*; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral