A map of the interactome network of the metazoan C. elegans.
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.
Li
Siming
S
Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
Armstrong
Christopher M
CM
Bertin
Nicolas
N
Ge
Hui
H
Milstein
Stuart
S
Boxem
Mike
M
Vidalain
Pierre-Olivier
PO
Han
Jing-Dong J
JD
Chesneau
Alban
A
Hao
Tong
T
Goldberg
Debra S
DS
Li
Ning
N
Martinez
Monica
M
Rual
Jean-François
JF
Lamesch
Philippe
P
Xu
Lai
L
Tewari
Muneesh
M
Wong
Sharyl L
SL
Zhang
Lan V
LV
Berriz
Gabriel F
GF
Jacotot
Laurent
L
Vaglio
Philippe
P
Reboul
Jérôme
J
Hirozane-Kishikawa
Tomoko
T
Li
Qianru
Q
Gabel
Harrison W
HW
Elewa
Ahmed
A
Baumgartner
Bridget
B
Rose
Debra J
DJ
Yu
Haiyuan
H
Bosak
Stephanie
S
Sequerra
Reynaldo
R
Fraser
Andrew
A
Mango
Susan E
SE
Saxton
William M
WM
Strome
Susan
S
Van Den Heuvel
Sander
S
Piano
Fabio
F
Vandenhaute
Jean
J
Sardet
Claude
C
Gerstein
Mark
M
Doucette-Stamm
Lynn
L
Gunsalus
Kristin C
KC
Harper
J Wade
JW
Cusick
Michael E
ME
Roth
Frederick P
FP
Hill
David E
DE
Vidal
Marc
M
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D019143:Evolution, Molecular; D017238:Genes, Helminth; D023281:Genomics; D016366:Open Reading Frames; D010641:Phenotype; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D029742:Caenorhabditis elegans Proteins; D020543:Proteome