Techniques to analyze the HIV-1 Tat and TAR RNA-dependent recruitment and activation of the cyclin T1: CDK9 (P-TEFb) transcription elongation factor.
Gomes
Nathan
N
Regulatory Biology Laboratory Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA.
Garber
Mitchell E
ME
Jones
Katherine A
KA
D001671:Biochemistry / Q000379:methods*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002845:Chromatography; D056906:Cyclin T; D016213:Cyclins / Q000737:chemistry; D015696:Gene Products, tat / Q000235:genetics*; D005982:Glutathione Transferase / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D042925:Positive Transcriptional Elongation Factor B / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry; D012319:RNA Polymerase II / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D015533:Transcriptional Activation; D054322:tat Gene Products, Human Immunodeficiency Virus
C503684:CCNT1 protein, human; D056906:Cyclin T; D016213:Cyclins; D015696:Gene Products, tat; D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D012313:RNA; D005982:Glutathione Transferase; D042925:Positive Transcriptional Elongation Factor B; D012319:RNA Polymerase II