title: [Detection of carbonic anhydrase IX gene expression in renal cell carcinoma with reverse transcription-PCR].
abstract: OBJECTIVE
  To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC).
  
  
  METHODS
  MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis.
  
  
  RESULTS
  Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues.
  
  
  CONCLUSIONS
  RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.
authors.affiliation: Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jyd@fimmu.com
authors.forename: Yao-dong
authors.initials: YD
authors.lastname: Jiang
authors.forename: Shao-bin
authors.initials: SB
authors.lastname: Zheng
authors.forename: Zhan-hui
authors.initials: ZH
authors.lastname: Wang
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D000951:Antigens, Neoplasm; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases