[Characteristics of cellular immunity in children with acute viral hepatitis A].
OBJECTIVE
To study the populational composition of lymphocytes and the specific features of production of cytokines in children with acute viral hepatitis A (AVHA).


METHODS
128 patients aged 11 to 14 years who had AVHA, moderate AVHA being in 83.5% were examined. In 87.2% of the children, the disease was cyclic. The etiology of the disease was verified by simultaneously detecting anti-HAV IgM (enzyme immunoassay) and by the presence of HAV RNA (polymerase chain reaction) in the blood. Peripheral lymphocytes (CD) were phenotypes in the indirect immunofluorescence test using monoclonal antibodies; cytokines were determined by the enzyme immunoassay; serum beta 2-microglobulin was done by radioimmunoassay.


RESULTS
The children with AVHA were found to have elevated levels of tumor necrosis beta-factor, interleukin-1 beta (IL-1 beta), and IL-4 in the icteric period, as well as a decrease in their levels at convalescence. IL-6 was detected in individual patients only in the first 3 days of the icteric period. The peak of AVHA was characterized by relative lymphocytosis, by decreases in the counts of T helper/inductor cells and natural killer cells, by increases in the count of CD25 cells, convalescence, by preserved lymphocytosis, by the increased levels of T lymphocytes that carry the markers CD3, CD4, CD8, CD16, CD25, and CD95, and by higher toxicity of beta 2-microglobulin.
Budarina
N A
NA
Belaia
O F
OF
Chulanov
V P
VP
Paĭmanov
N V
NV
Pak
S G
SG
D000208:Acute Disease; D000293:Adolescent; D015703:Antigens, CD / Q000276:immunology*; D002648:Child; D006506:Hepatitis A / Q000097:blood / Q000276:immunology* / Q000821:virology; D035921:Hepatitis A Antigens / Q000276:immunology*; D017957:Hepatitis A Virus, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007375:Interleukin-1 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D007378:Interleukins / Q000097:blood / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D001613:beta 2-Microglobulin / Q000097:blood / Q000276:immunology
D015703:Antigens, CD; D035921:Hepatitis A Antigens; D007375:Interleukin-1; D007378:Interleukins; D012367:RNA, Viral; D014409:Tumor Necrosis Factor-alpha; D001613:beta 2-Microglobulin; D015847:Interleukin-4