Effect of age on immune parameters and the immune response of dogs to vaccines: a cross-sectional study.
The evaluation of anti-aging intervention strategies in dogs would benefit from reliable quantitative biomarkers of aging. In the present study, the expression of various immune parameters was measured in young and old dogs to identify potential biomarkers of aging. The second goal of the study was to determine the effect of age on the immune response to vaccines. The immune function, including the antibody response to vaccines, was determined in 32 young adult (3.15+/-0.8 years of age) and 33 old dogs (12.1+/-1.3 years of age) of various breeds. Old dogs had a significantly lower lymphocyte proliferative response and a lower percentage of CD4+ T cells and CD45R+/CD4+ T cells, and a higher percentage of CD8+ T cells and a higher concentration of serum and salivary IgA. The most significant differences (P<0.001) occurred in the lymphocyte proliferative responses to ConA and PHA, the CD4:CD8 ratio, and the percentage of CD45R+/CD4+ T cells suggesting that these parameters are potential biomarkers of aging. There was no difference in the percentage of total T and B lymphocytes and the concentration of serum IgM and IgG. Both groups of dogs had protective titers against distemper virus, parvovirus and rabies virus before annual revaccination. The pre-vaccination titer against rabies virus was higher in the old dogs than in the young dogs, and there were no differences in post-vaccination titers against any of the viruses. This suggests that annual vaccination protocols provide adequate protection for old dogs.
HogenEsch
Harm
H
Department of Veterinary Pathobiology, Purdue University, Veterinary Pathology Building, 725 Harrison Street, West Lafayette, IN 47907-2027, USA. hogenesch@purdue.edu
Thompson
Steven
S
Dunham
Anisa
A
Ceddia
Michael
M
Hayek
Michael
M
D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003430:Cross-Sectional Studies; D004285:Dogs / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007132:Immunoglobulin Isotypes / Q000097:blood; D016130:Immunophenotyping / Q000662:veterinary; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D008297:Male; D008934:Mitogens / Q000276:immunology; D009500:Neutralization Tests / Q000662:veterinary; D011819:Rabies Vaccines / Q000276:immunology / Q000494:pharmacology; D012463:Saliva / Q000276:immunology / Q000821:virology; D014611:Vaccination / Q000662:veterinary*
D000914:Antibodies, Viral; D007132:Immunoglobulin Isotypes; D008934:Mitogens; D011819:Rabies Vaccines