Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose.
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% +/- 7% on Matrigel. Lipid volume surpassed 4.5 x 10(3) microm3 cell(-1) for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3 x 10(3) microm2 cell(-1). These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
O'Connor
Kim C
KC
Department of Chemical Engineering, Tulane University, Boggs Center, Room 300, New Orleans, LA 70118, USA. koc@tulane.edu
Song
Hong
H
Rosenzweig
Nitsa
N
Jansen
David A
DA
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000328:Adult; D001940:Breast / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D005240:Feasibility Studies; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008875:Middle Aged; D011509:Proteoglycans / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D004338:Drug Combinations; D005353:Fibronectins; D007797:Laminin; D008055:Lipids; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen