Biotelemetry transmitter implantation in rodents: impact on growth and circadian rhythms.
The implantation of a biotelemetry transmitter for core body temperature (T(c)) and motor activity (MA) measurements is hypothesized to have effects on growth and circadian rhythmicity depending on animal body-to-transmitter (B:T) size ratio. This study examined the impact of transmitter implantation (TM) on body weight, food intake (FI), water intake (WI), and circadian T(c) and MA rhythms in mice (23.8 +/- 0.04 g) and rats (311.5 +/- 5.1 g) receiving no treatment (NT), anesthesia, laparotomy (LAP), and TM. The B:T size ratio was 6:1 and 84:1 for mice and rats, respectively. In mice, body weight required 14 days to recover to presurgical levels and never attained the level of the other groups. FI recovered in 3 days, whereas WI never reached presurgical levels. Rat body weight did not decrease below presurgical levels. FI and WI recovered to presurgical levels in rats by day 2 postsurgery. Anesthesia decreased mouse body weight for 1 wk, but was without effect in rats. LAP significantly decreased body weight for 5 days in mice and 1 day in rats, showing a significant effect of the surgical procedure in the absence of TM in both species. Circadian T(c) and MA rhythms were evident within the first week in both species, indicating dissociation between circadian rhythmicity and recovery of growth variables. Cosinor analysis showed a TM effect on T(c) min, T(c) max, mesor, amplitude, and period of mice, whereas only the amplitude of the rhythm was affected in rats. These data indicate that a large B:T size ratio is associated with minimization of the adverse effects of surgical implantation. We recommend that B:T size ratio, recovery of presurgical body weight, and display of a robust circadian T(c) and MA rhythm be established before collection of biotelemetry data collection under an experimental paradigm.
Leon
Lisa R
LR
U.S. Army Research Institute of Environmental Medicine, Thermal and Mountain Medicine Division, 42 Kansas Street, Natick, MA 01760-5007, USA. lisa.leon@na.amedd.army.mil
Walker
Larry D
LD
DuBose
David A
DA
Stephenson
Lou A
LA
D000818:Animals; D001824:Body Constitution; D001831:Body Temperature / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D004326:Drinking / Q000502:physiology; D004435:Eating / Q000502:physiology; D020007:Implants, Experimental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000254:growth & development / Q000502:physiology*; D013686:Telemetry / Q000295:instrumentation* / Q000379:methods*