title: Prolactin prevents acute stress-induced hypocalcemia and ulcerogenesis by acting in the brain of rat.
abstract: Stress causes hypocalcemia and ulcerogenesis in rats. In rats under stressful conditions, a rapid and transient increase in circulating prolactin (PRL) is observed, and this enhanced PRL induces PRL receptors (PRLR) in the choroid plexus of rat brain. In this study we used restraint stress in water to elucidate the mechanism by which PRLR in the rat brain mediate the protective effect of PRL against stress-induced hypocalcemia and ulcerogenesis. We show that rat PRL acts through the long form of PRLR in the hypothalamus. This is followed by an increase in the long form of PRLR mRNA expression in the choroid plexus of the brain, which provides protection against restraint stress in water-induced hypocalcemia and gastric erosions. We also show that PRL induces the expression of PRLR protein and corticotropin-releasing factor mRNA in the paraventricular nucleus. These results suggest that the PRL levels increase in response to stress, and it moves from the circulation to the cerebrospinal fluid to act on the central nervous system and thereby plays an important role in helping to protect against acute stress-induced hypocalcemia and gastric erosions.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Mie University, Japan. t-fuji@doc.medic.mie-u.ac.jp
authors.forename: Takahiko
authors.initials: T
authors.lastname: Fujikawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Soya
authors.forename: Kellie L K
authors.initials: KL
authors.lastname: Tamashiro
authors.forename: Randall R
authors.initials: RR
authors.lastname: Sakai
authors.forename: Bruce S
authors.initials: BS
authors.lastname: McEwen
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakai
authors.forename: Masato
authors.initials: M
authors.lastname: Ogata
authors.forename: Ikukatsu
authors.initials: I
authors.lastname: Suzuki
authors.forename: Kunio
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000208:Acute Disease; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002831:Choroid Plexus / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D006996:Hypocalcemia / Q000209:etiology / Q000517:prevention & control*; D007101:Immersion; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D008297:Male; D008968:Molecular Conformation; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D011388:Prolactin / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011981:Receptors, Prolactin / Q000737:chemistry / Q000235:genetics; D012149:Restraint, Physical; D013276:Stomach Ulcer / Q000209:etiology / Q000517:prevention & control*; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011388:Prolactin; D003346:Corticotropin-Releasing Hormone