Increased electronegative charge of serum low-density lipoprotein in patients with diabetes mellitus.
BACKGROUND
Patients with diabetes mellitus have been reported to show increased serum levels of modified low-density lipoprotein (LDL), including glycosylated, oxidized and small, dense LDL. This change has been suggested to represent an important risk factor for diabetic macroangiopathy. A common characteristic shared by these modified LDL species is the increase in electronegative charge on particle surfaces, which can be detected by agarose gel electrophoresis as "LDL charge modified frequency" (LDL-CMF) determined from the relative mobility of LDL fraction.


METHODS
LDL-CMF was measured in the sera from 129 outpatients with type 2 diabetes mellitus and compared with the data from 34 normal subjects.


RESULTS
The LDL fraction from diabetics migrates more closely to the anode side as compared with that from normal subjects. The LDL-CMF measured in diabetics, 5.5+/-8.1%, was significantly (p<0.0001) higher than 0.6+/-3.4% in normal subjects. Serum LDL-CMF showed significant positive correlations with triglyceride at r=0.552 (p<0.0001) and malondialdehyde modified LDL at r=0.390 (p<0.0001), as well as systolic blood pressure, body mass index, fasting plasma glucose, hemoglobin A(1c), total cholesterol, free fatty acid (FFA) and homeostasis model assessment ratio. It showed negative correlations with high-density lipoprotein and total superoxide dismutase activity.


CONCLUSIONS
The results indicate that LDL-CMF reflects the degree of serum LDL modification in diabetics and can be regarded as an important risk factor for diabetic macroangiopathy.
Yano
Masao
M
Central Clinical Laboratories, Mitsui Memorial Hospital, 1 Kanda Izumi-cho, Tokyo 101-8643, Chiyoda-ku, Japan. yano-m@mitsuihosp.or.jp
Inoue
Minoru
M
Maehata
Eisuke
E
Shiba
Teruo
T
Yamakado
Minoru
M
Hirabayashi
Yoji
Y
Taniyama
Matsuo
M
Suzuki
Seiji
S
D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood* / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000097:blood*; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010084:Oxidation-Reduction; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D008315:Malondialdehyde; D013482:Superoxide Dismutase