Methods to detect antifibrillarin antibodies in patients with systemic sclerosis (SSc): a comparison.
Autoantibodies against nucleolar antigens are common in systemic sclerosis (SSc). They include autoantibodies against fibrillarin (Fb), which are serological markers for SSc. Fb is associated with the evolutionally-conserved box C/D of small nucleolar RNAs (snoRNAs). We compared indirect immunofluorescence (IIF), Western blot (WB), and immunoprecipitation (IPP) of total small RNAs assays to determine which of these techniques is most specific for the detection of snoRNPs. We also examined the frequency and specificity of autoantibodies from SSc patients to snoRNAs, snRNAs, and scRNAs, and concluded that 1) IIF can not determine autoantibody specificity against Fb, 2) 36% of SSc sera were false-negative by WB, and 3) by IPP, anti-Fb autoantibodies from SSc patients can bind U3, U8, U13, U15, and U22 snoRNAs.
García
Josefina Huerta
JH
Department of Molecular Biochemistry, Centro de Biología Experimental, Universidad Autónoma de Zacatecas, Guadalupe, Mexico.
Osuna
Monica Delgado
MD
Castrejon
Filiberto Martinez
FM
Enriquez
Laura Guzman
LG
Reyes
Pedro A
PA
Hermosillo
J Jesus Cortes
JJ
D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D015153:Blotting, Western; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007159:Immunologic Tests; D011233:Precipitin Tests; D020537:RNA, Small Nucleolar / Q000032:analysis; D012595:Scleroderma, Systemic / Q000276:immunology*
D001323:Autoantibodies; D002868:Chromosomal Proteins, Non-Histone; D020537:RNA, Small Nucleolar; C047458:fibrillarin