In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation.
The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.
Zhu
Jie
J
Department of Gene Expression and Development, The Roslin Institute, Midlothian, EH 25 9PS, Scotland, United Kingdom.
King
Tim
T
Dobrinsky
John
J
Harkness
Linda
L
Ferrier
Tricia
T
Bosma
Wim
W
Schreier
Lori L
LL
Guthrie
H David
HD
DeSousa
Paul
P
Wilmut
Ian
I
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D046508:Culture Techniques; D004558:Electric Stimulation; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000502:physiology*; D010060:Ovulation; D010312:Parthenogenesis / Q000502:physiology; D011247:Pregnancy; D013552:Swine / Q000196:embryology*