title: [DNA microarray for the detection of Yersinia pesits].
abstract: OBJECTIVE
  To develop a DNA microarray technique for fast diagnosis of plague.
  
  
  METHODS
  Restriction display polymerase chain reaction (RD-PCR) and hybridization with fluorescently labeled cy5 were employed for detecting the sample DNA of Yersinia pestis, Yersinia pseudotuberculosis and Yersinia enterocolitica.
  
  
  RESULTS
  The prepared microarray is capable of distinguishing Yersinia pestis from Yersinia pseudotuberculosis and Yersinia enterocolitica in the same genus.
  
  
  CONCLUSIONS
  The constructed DNA microarray is an effective diagnostic tool for the detection of Yersinia pestis.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
authors.forename: Xing-qi
authors.initials: XQ
authors.lastname: Dong
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D005453:Fluorescence; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015010:Yersinia pestis / Q000302:isolation & purification*