Functional differentiation of peroxisomes revealed by expression profiles of peroxisomal genes in Arabidopsis thaliana.
It is well known that peroxisomal matrix proteins contain one of two targeting signals, PTS1 and PTS2. We comprehensively surveyed genes related to peroxisomal function and biogenesis in the entire Arabidopsis genome sequence. Here, we identified 256 gene candidates of PTS1- and PTS2-containing proteins and another 30 genes of non-PTS-containing proteins. Of these, only 29 proteins have been reported to be functionally characterized as peroxisomal proteins in higher plants. We extensively investigated expression profiles of genes described above in various organs of Arabidopsis: Statistical analyses of these expression profiles revealed that peroxisomal genes could be divided into five groups. One group showed ubiquitous expression in all organs examined, while the other four were classified as showing organ-specific expression in seedlings, cotyledons, roots and in both cotyledons and leaves. These data proposed more detailed description of differentiation of plant peroxisomes.
Kamada
Tomoe
T
Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan.
Nito
Kazumasa
K
Hayashi
Hiroshi
H
Mano
Shoji
S
Hayashi
Makoto
M
Nishimura
Mikio
M
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018548:Cotyledon / Q000235:genetics / Q000502:physiology; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D006038:Glyoxylates / Q000378:metabolism; D020676:Glyoxysomes / Q000235:genetics / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000145:classification / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018514:Plant Structures / Q000235:genetics / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D004798:Enzymes; D006038:Glyoxylates; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; C031150:glyoxylic acid