Inhibition of matrix metalloproteinase-2 activity by siderophores of Pseudomonas species.
To obtain a novel matrix metalloproteinase (MMP) inhibitor produced by bacteria, we have focused on the chelating activity of siderophores. Several siderophore-producing bacteria were isolated from soil using chrome azurol S agar plates and then the effect of siderophores on MMP-2 activity was assayed by gelatin zymography. The results showed that partially purified siderophores from ten isolated strains inhibited MMP-2 activity. Among these strains, two were non-fluorescent and eight were fluorescent Pseudomonas species. From these eight strains, pyoverdine-type siderophores were detected. The Zn(2+)-chelating activity of these siderophores correlated with the inhibition of MMP-2 activity. Therefore, it is considered that siderophores such as pyoverdines inhibit MMP-2 activity by chelating Zn(2+) on the active site of MMP-2.
Shinozaki
Y
Y
Institute of Applied Biochemistry, University of Tsukuba, 305-8572 Tsukuba, Ibaraki, Japan.
Akutsu-Shigeno
Y
Y
Nakajima-Kambe
T
T
Inomata
S
S
Nomura
N
N
Nakahara
T
T
Uchiyama
H
H
D001665:Binding Sites; D002853:Chromatography, Liquid; D062385:Hydroxybenzoates; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D015032:Zinc / Q000737:chemistry
D062385:Hydroxybenzoates; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides; D017262:Siderophores; C015076:chrome azurol S; C042453:pyoverdin; D020778:Matrix Metalloproteinase 2; D015032:Zinc