title: Comparing genomic expression patterns across species identifies shared transcriptional profile in aging.
abstract: We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging, and show that similar analysis can be applied to a range of biological processes. We found that two highly diverged animals, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, implement a shared adult-onset expression program of genes involved in mitochondrial metabolism, DNA repair, catabolism, peptidolysis and cellular transport. Most of these changes were implemented early in adulthood. Using this approach to search databases of gene expression data, we found conserved transcriptional signatures in larval development, embryogenesis, gametogenesis and mRNA degradation.
authors.affiliation: Program in Neuroscience, University of California, San Francisco, California 94143, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: McCarroll
authors.forename: Coleen T
authors.initials: CT
authors.lastname: Murphy
authors.forename: Sige
authors.initials: S
authors.lastname: Zou
authors.forename: Scott D
authors.initials: SD
authors.lastname: Pletcher
authors.forename: Chen-Shan
authors.initials: CS
authors.lastname: Chin
authors.forename: Yuh Nung
authors.initials: YN
authors.lastname: Jan
authors.forename: Cynthia
authors.initials: C
authors.lastname: Kenyon
authors.forename: Cornelia I
authors.initials: CI
authors.lastname: Bargmann
authors.forename: Hao
authors.initials: H
authors.lastname: Li
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013997:Time Factors