title: Method of detecting beta-lactam antibiotic induced vancomycin resistant MRSA (BIVR).
abstract: Despite the fact that the combination of vancomycin and a beta-lactam antibiotic are known to act synergistically on vancomycin-susceptible Staphylococcus aureus (VSSA), some MRSA have emerged showing antagonism to the combination of vancomycin and a beta-lactam antibiotic. These MRSA are called beta-lactam antibiotic-induced vancomycin resistant MRSA (BIVR). A method based on this antagonistic phenomenon has been devised to detect BIVR strains. The method inhibits the VSSA strain but allows the BIVR strain to grow. Forty-six commercially available beta-lactam antibiotics induced the vancomycin-resistance. Using this detection method, 717 MRSA clinical isolates obtained from eight institutes throughout Japan were thus screened and 6.3% of these were detected as BIVR when judged at 48 h.
authors.affiliation: Department of Infection Control Lab, The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-0072, Japan. hanaki@lisci.kitasaro-u.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hanaki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Syuichi
authors.initials: S
authors.lastname: Nomura
authors.forename: Isao
authors.initials: I
authors.lastname: Haraga
authors.forename: Ariaki
authors.initials: A
authors.lastname: Nagayama
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sunakawa
mesh_terms: D004336:Drug Antagonism; D006801:Humans; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000502:physiology*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D047090:beta-Lactams; D014640:Vancomycin