Applications of laser-polarized 129Xe to biomolecular assays.
The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and functionalized 129Xe NMR to in vitro biomolecular assays suggests the potential future use of a functionalized xenon biosensor for in vivo imaging.
Lowery
Thomas J
TJ
Department of Chemistry, University of California at Berkeley, Berkeley, CA 94720, USA.
Rubin
Seth M
SM
Ruiz
E Janette
EJ
Spence
Megan M
MM
Winssinger
Nicolas
N
Schultz
Peter G
PG
Pines
Alexander
A
Wemmer
David E
DE
D006801:Humans; D007834:Lasers; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011487:Protein Conformation; D014979:Xenon Isotopes
D008024:Ligands; D014979:Xenon Isotopes