Differential expression of genes related to levels of mucosal cell proliferation among multiple rat strains by using oligonucleotide microarrays.
Induction levels of cell proliferation, in response to gastric mucosal damage by N-methyl- N'-nitro- N-nitrosoguanidine (MNNG), are different among rat strains and correlate with susceptibility to MNNG-induced gastric carcinogenesis. Here, we used oligonucleotide microarrays to search for genes that show expression levels accordant with the extents of cell proliferation among six rat strains. Expression levels of 8,800 probe sets were analyzed in the pylorus of ACI, LEW, WKY (strains with strong cell proliferation), F344, (ACI x BUF)F1, and BUF rats (strains with weak cell proliferation) after 2-week MNNG treatment. No genes showed complete accordance, and 22 genes showed accordance with one or two exceptions. After confirmation by quantitative RT-PCR, four genes--cellular retinoic acid-binding protein II ( Crabp2), fatty acid binding protein 1 ( Fabp1), progastricsin (pepsinogen C, Pgc), and UDP-glucuronosyltransferase 2 family member 5 ( Ugt2b5)--were found to show good accordance with only one exception. Crabp2, Fabp1, and Ugt2b5 were differentially expressed between ACI and BUF rats both before and after MNNG treatment. Although Crabp2 had been identified as one of the 16 genes differentially expressed between ACI and BUF rats with cDNA-RDA, Fabp1 and Ugt2b5 were newly identified in this study. All three genes are known to be involved in retinoic acid-mediated signaling and could be involved in the control of differential induction of cell proliferation.
Yamashita
Satoshi
S
Carcinogenesis Division, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
Nomoto
Tomoko
T
Ohta
Tsutomu
T
Ohki
Misao
M
Sugimura
Takashi
T
Ushijima
Toshikazu
T
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002455:Cell Division; D017931:DNA Primers; D050556:Fatty Acid-Binding Proteins; D020869:Gene Expression Profiling; D014453:Glucuronosyltransferase / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats / Q000235:genetics*; D011922:Rats, Mutant Strains; D018168:Receptors, Retinoic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D014212:Tretinoin
D002352:Carrier Proteins; D017931:DNA Primers; C493321:Fabp1 protein, rat; D050556:Fatty Acid-Binding Proteins; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D008769:Methylnitronitrosoguanidine; D014212:Tretinoin; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D014453:Glucuronosyltransferase; C091152:Gstt2 protein, rat; D005982:Glutathione Transferase