Improved function of rat islets upon co-microencapsulation with Sertoli's cells in alginate/poly-L-ornithine.
The purpose of this study was to assess whether Sertoli's cells would improve functional performance of homologous pancreatic islets within microcapsules. Purified rat Sertoli's cells were co-enveloped with islets in microcapsules that had been fabricated with alginic acid and poly-L-ornithine. Confocal laser microscopy was used to determine any mitogenic effects of Sertoli's cells on islets beta-cells. Insulin secretion from islets, with or without Sertoli's cells, was examined, and grafts of Sertoli's cells with islets in microcapsules into diabetic mice were carried out. Co-incubation of Sertoli's cells with islets resulted in a significant increase in the islet beta-cell mitotic rate, which was coupled with significantly higher insulin release under glucose stimulation, as compared to controls. Grafts of co-microencapsulated Sertoli's cells with islets resulted in prolongation of the achieved normoglycemia in the animals receiving Sertoli's cells with islets as compared to controls that received islets only. Sertoli's cells do promote mitogenic activities upon in vitro co-incubation with islets, whose in vitro functional and in vivo post-transplant consequences were evident. Sertoli's cells could, therefore, be co-microencapsulated with islets for transplantation in diabetic recipients.
Luca
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G
Department of Chemistry and Pharmaceutical Technology of Drugs, University of Perugia, Perugia, Italy. luca@dimisem.med.unipg.it
Calafiore
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R
Basta
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G
Ricci
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M
Calvitti
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M
Neri
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L
Nastruzzi
C
C
Becchetti
E
E
Capitani
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Brunetti
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Rossi
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C
D000464:Alginates; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism / Q000648:ultrastructure; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051379:Mice; D008938:Mitosis; D010455:Peptides; D051381:Rats; D012708:Sertoli Cells / Q000502:physiology / Q000637:transplantation*
D000464:Alginates; D006603:Hexuronic Acids; D007328:Insulin; D010455:Peptides; C008973:polyornithine; D020723:Glucuronic Acid