Transgenic tobacco and apple plants expressing biotin-binding proteins are resistant to two cosmopolitan insect pests, potato tuber moth and lightbrown apple moth, respectively.
Tobacco (Nicotiana tabacum cv. Samsun) and apple (Malus x domestica cv. Royal Gala) plants expressing avidin or strepavidin were produced using Agrobacterium tumefaciens-mediated transformation. ELISA assays showed that avidin expression ranged from 3.1 to 4.6 microM in tobacco and from 1.9 to 11.2 microM in apple and streptavidin expression ranged from 11.4 to 24.5 microM in tobacco and from 0.4 to 14.6 microM in apple. Expressed at these levels, both biotin-binding proteins conferred a high level of insect resistance on transformed tobacco plants to larval potato tuber moth (PTM), Phthorimaea operculella (Zeller) (fam. Gelechiidae) and on apple plants to larvae of the lightbrown apple moth (LBAM) Epiphyas postvittana (Walker) (fam. Tortricidae). More than 90% of PTM larvae died on tobacco plants expressing either avidin or streptavidin genes within 9 days of inoculation. Mortality of LBAM larvae was significantly higher (P < 0.05) on three avidin-expressing (89.6, 84.9 and 80.1%) and two streptavidin-expressing (90 and 82.5%) apple plant lines than on non-transformed control plants (14.1%) after 21 days. Weight of LBAM larvae was also significantly reduced by feeding on all apple shoots expressing avidin and on apple shoots expressing streptavidin at levels of 3.8 microM and above.
Markwick
Ngaire P
NP
The Horticulture and Food Research Institute of New Zealand Ltd, 120 Mt Albert Road, Auckland, New Zealand. nmarkwick@hortresearch.co.nz
Docherty
Lisa C
LC
Phung
Margaret M
MM
Lester
Melissa T
MT
Murray
Colleen
C
Yao
Jia-Long
JL
Mitra
Deepali S
DS
Cohen
Daniel
D
Beuning
Lesley L
LL
Kutty-Amma
Sumathi
S
Christeller
John T
JT
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000818:Animals; D001360:Avidin / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D027845:Malus / Q000235:genetics* / Q000469:parasitology; D009036:Moths / Q000235:genetics / Q000378:metabolism; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D019809:Streptavidin / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000469:parasitology; D014170:Transformation, Genetic
D002352:Carrier Proteins; C017071:biotin-binding proteins; D001360:Avidin; D019809:Streptavidin