Microcapsules based on glycinin-sodium dodecyl sulfate complex coacervation.
Microcapsules processed by complex coacervation were prepared using hexadecane for the oil phase and glycinin (a soybean storage protein)-sodium dodecyl sulfate (SDS) as the main wall-forming material. The study underlines the essential role of SDS, which, by the way of [glycinin(+)-SDS(-)] insoluble complex formation, allowed the precipitation of proteins around oil droplets. Moreover, particular attention was attributed to the study of suitable conditions of glycinin cross-linking with glutaraldehyde. The reticulation step was performed at pH 4.0 and it was observed that the precipitated state of proteins increased considerably the efficiency of the cross-linking reaction. Analysis of the reactional medium after each main step of the process (emulsification, complex coacervation, cross-linking) allowed the follow-up and characterization of microcapsule formation. Optimization of different process parameters such as glycinin concentration, glycinin/SDS/glutaraldehyde ratios, pH and the kinetics of cross-linking allowed the encapsulation of the totality of oil and the use of more than 98% of initially introduced proteins for the microcapsule wall formation.
Lazko
J
J
GEPEA UMR-CNRS 6144, BP 406, F-44602 St-Nazaire Cedex, France.
Popineau
Y
Y
Renard
D
D
Legrand
J
J
D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems / Q000379:methods; D005916:Globulins / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012995:Solubility; D030262:Soybean Proteins
D002214:Capsules; D005916:Globulins; D030262:Soybean Proteins; D012967:Sodium Dodecyl Sulfate; C011474:glycinin