Measurement of red cell velocity in microvessels using particle image velocimetry (PIV).
A new technique using particle image velocimetry (PIV) has been developed to evaluate the detailed velocity profiles of red cells flowing in microvessels. The microcirculation in rat mesentery was directly observed using high-speed videomicroscopy, and the images of red cells flowing in the mesenteric arterioles were recorded simultaneously with the arterial blood pressure. Based on the high-speed videomicroscopic images obtained, velocity vectors in single or branched arterioles were evaluated to obtain velocity profiles across the cross-section of arterioles. It was shown that in single and straight arterioles the velocity profile was blunt with a pit at the central region, and its pit was marked in bifurcation. The present technique enables us to analyze red cell velocity profiles up to 0.8 microm in the spatial resolution and 1 msec in the time interval.
Nakano
Atushi
A
Department of Vascular Physiology, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan. nakano@ri.ncvc.go.jp
Sugii
Yasuhiko
Y
Minamiyama
Motomu
M
Niimi
Hideyuki
H
D000818:Animals; D001160:Arterioles; D001783:Blood Flow Velocity; D018056:Hemorheology / Q000295:instrumentation*; D007091:Image Processing, Computer-Assisted; D008297:Male; D008833:Microcirculation; D018715:Microscopy, Video; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation