title: Investigations into an outbreak of tuberculosis in a flock of sheep in contact with tuberculous cattle.
abstract: Tuberculosis, associated with Mycobacterium bovis infection, occurs infrequently in sheep. A sheep flock, which was potentially exposed to a high level of infection from in-contact tuberculous cattle, was examined for evidence of infection. Six sheep that had given a positive reaction to the comparative intradermal tuberculin test were examined post mortem. Tuberculous lesions were present in four of these sheep. Lesion morphology and distribution in the sheep was similar to that in cattle. M. bovis was cultured from the lesions and the isolates were strain typed by spoligotyping and variable number of tandem repeats (VNTR) typing. Tuberculin-reacting sheep also reacted positively to an assay for in vitro release of interferon-gamma. This paper describes the first report of an outbreak of tuberculosis in sheep in either Britain or Ireland. The report describes immunology and pathology findings and, using molecular typing techniques, suggests that the sheep had been infected from in-contact cattle.
authors.affiliation: Veterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland, 43 Beltany Road, Omagh, County Tyrone, BT78 5NF, UK. frank.malone@dardni.gov.uk
authors.forename: F E
authors.initials: FE
authors.lastname: Malone
authors.forename: E C
authors.initials: EC
authors.lastname: Wilson
authors.forename: J M
authors.initials: JM
authors.lastname: Pollock
authors.forename: R A
authors.initials: RA
authors.lastname: Skuce
mesh_terms: D000818:Animals; D002417:Cattle; D003612:Dairying; D004196:Disease Outbreaks / Q000662:veterinary*; D018562:Disease Transmission, Infectious / Q000662:veterinary*; D005260:Female; D007371:Interferon-gamma / Q000097:blood; D007428:Intradermal Tests / Q000662:veterinary; D007494:Ireland / Q000453:epidemiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000635:transmission*; D014380:Tuberculosis, Bovine / Q000635:transmission*
chemical_list: D007371:Interferon-gamma