Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin.
Two new genes in the cytochrome P450 (CYP6) family 6 with complete coding sequences were cloned and sequenced from deltamethrin-resistant Anopheles minimus, a major malaria vector in Thailand. CYP6P5 encodes a protein of 508 amino acids, while CYP6AA2 contains 505 residues. Each encoded protein contains a hydrophobic N-terminal region and a highly conserved heme-binding region typical of P450s. Alignments of deduced amino acid sequences with other insect P450 genes indicate a high degree of identity to insect CYP6 genes. Comparative mRNA expression studies using semi-quantitative RT-PCR analysis indicated that the relative amount of CYP6AA2 transcript was greater in the deltamethrin-resistant An. minimus compared to the susceptible strain. The expression of CYP6AA2 in deltamethrin-resistant mosquitoes is associated with development of deltamethrin resistance in An. minimus mosquito. The CYP6P5 transcript is equally expressed in both resistant and susceptible mosquitoes.
Rongnoparut
Pornpimol
P
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Boonsuepsakul
Soamrutai
S
Chareonviriyaphap
Theeraphap
T
Thanomsing
Naowarat
N
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004351:Drug Resistance; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009570:Nitriles; D011722:Pyrethrins / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System