Cloning and sequence analysis of the Candida utilis HIS3 gene.
A DNA fragment, carrying the Candida utilis HIS3 gene, has been isolated from a genomic DNA library by complementation of the E. coli hisB mutant. Its nucleotide sequence was determined and it predicts a single open reading frame of 675 bp (224 aa). The deduced amino acid sequence is highly homologous to other yeast and fungi HIS3 genes.
Basabe
Liliana
L
Center for Genetic Engineering and Biotechnology, Agriculture Division, 31 Ave /158 & 190, PO Box 6162, Havana 10600, Cuba. liliana.basabe@cigb.edu.cu
Domínguez
Iveldris
I
Chávez
Francisco P
FP
D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase