title: Polymerase chain reaction for detection of patent infections of Echinococcus granulosus ("sheep strain") in naturally infected dogs.
abstract: Polymerase chain reaction (PCR) for the identification of eggs of the tapeworm Echinococcus granulosus ("sheep strain") was evaluated with primers derived from mitochondrial sequences. Specificity of these primers was confirmed by investigating DNA of other strains of E. granulosus and of 14 helminth species which inhabit the intestines of dogs. This PCR assay was used to investigate 131 purged dogs from Kazakhstan. Eighteen dogs harboured Echinococcus worms, ten of them in mixed infections with Taenia spp. Coproantigen detection was positive in 15 and taeniid eggs could be recovered from 13 of these specimens. Eight of the egg-containing samples were positive in the PCR for E. granulosus and four in a Echinococcus multilocularis -specific PCR revealing one mixed infection. Egg-containing faeces from two dogs harbouring both Taenia spp. and Echinococcus spp. were negative in both PCRs. The combination of egg isolation and PCR will also be of value in epidemiological studies when investigating environmental samples.
authors.affiliation: Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland.
authors.forename: Sasa
authors.initials: S
authors.lastname: Stefanić
authors.forename: Block S
authors.initials: BS
authors.lastname: Shaikenov
authors.forename: Peter
authors.initials: P
authors.lastname: Deplazes
authors.forename: Anke
authors.initials: A
authors.lastname: Dinkel
authors.forename: Paul R
authors.initials: PR
authors.lastname: Torgerson
authors.forename: Alexander
authors.initials: A
authors.lastname: Mathis
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis / Q000662:veterinary*; D004446:Echinococcus / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013622:Taeniasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial