title: Global analysis of shear stress-responsive genes in vascular endothelial cells.
abstract: DNA microarray gene expression analysis was conducted in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) exposed to laminar or turbulent shear stress. Approximately 3% of the total 5600 gene in HUVECs and HCAECs increased their expression more than two-fold or decreased it to less than half the static control in response to an arterial level of laminar shear stress (15 dynes/cm(2) for 24 hours). The proportions of shear-stress-responsive genes decreased to around 2% under the venous level of laminar shear stress (1.5 dynes/cm(2)) in both cell lines. Turbulent shear stress of 1.5 dynes/cm(2) altered the expression of 1.1% of all genes in the HCAECs. Laminar shear stress, but not turbulent shear stress, decreased the expression of a number of genes involved in DNA synthesis and the cell cycle in both HUVECs and HCAECs. Clustering analysis showed a variety of temporal profiles of gene expression in HUVECs exposed to laminar shear stress of 15 dynes/cm(2) for 3, 6, 12, 24, and 48 hours. Turbulent shear stress affected expression of many genes that play a role in vascular remodeling, including genes encoding plasminogen activators and their inhibitor, endothelin-1, transforming growth factor-beta, collagen type IV, and ephrin A1.
authors.affiliation: Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Ohura
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Sigeru
authors.initials: S
authors.lastname: Ichioka
authors.forename: Takaaki
authors.initials: T
authors.lastname: Sokabe
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakatsuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Baba
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shibata
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakatsuka
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Harii
authors.forename: Youichiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Takahide
authors.initials: T
authors.lastname: Kohro
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Joji
authors.initials: J
authors.lastname: Ando
mesh_terms: D001161:Arteriosclerosis / Q000235:genetics* / Q000503:physiopathology*; D002478:Cells, Cultured; D016000:Cluster Analysis; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011673:Pulsatile Flow; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology