Whole blood samples as an alternative to serum for detection of immunity to measles virus by ELISA.
The aim of this study was to evaluate enzyme linked immunosorbent assay (ELISA) as a testing strategy for detection of antibodies against measles virus from microquantities of blood soaked onto filter paper. We studied 165 healthy children in the age group of 1 to 2 years, attending the outpatient department of pediatrics. Two sets of samples were collected from each child. One by venipuncture and the other on Whatman filter paper-3 discs of 20 mm size by finger or heel prick so that each strip is completely soaked with blood on both sides. These were tested for measles virus antibodies by ELISA using Melotest measles IgG commercial ELISA kit manufactured by Melotec S. A. (Barcelona, Spain). The resulting sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the filter paper (FP) ELISA compared to serum ELISA was 100, 90, 97.8, and 100%, respectively. The correlation coefficient r = 0.93% (p < 0.001) and the agreement between the two techniques was 98% as calculated by the Kappa statistical method. The present study has found filter paper testing by ELISA to be a promising qualitative technique for detection of immunity against measles.
Chakravarti
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A
Department of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospital, New Delhi, India. dochak@yahoo.com
Rawat
D
D
Yadav
Sangita
S
D000914:Antibodies, Viral / Q000032:analysis*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007109:Immunity / Q000502:physiology; D008297:Male; D008457:Measles / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D008459:Measles virus / Q000276:immunology*; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000379:methods
D000914:Antibodies, Viral