Effect of phosphophoryn on rhBMP-2-induced bone formation.
In the present study, dentin phosphophoryn (DPP) derived from fresh bovine dentin was evaluated as a co-factor for recombinant human bone morphogenetic protein-2 (rhBMP-2) in rhBMP-2-induced bone formation in rats. A 5 microg amount of Escherichia coli-derived rhBMP-2 variant was combined with DPP cross-linked to type I collagen (2.4 microg DPP/360 microg collagen), acting as carrier. Next, rhBMP-2/DPP/collagen composites were implanted by onlay-grafting beneath the cranial periosteum in 4-week-old Wistar rats. Rats were sacrificed at 2 and 3 weeks after implantation. Throughout the experimental period, rhBMP-2/DPP/collagen composite induced more bone formation than the rhBMP-2/collagen composite. Moreover, the degradation rate of rhBMP-2/DPP/collagen composite in rat was faster than that of rhBMP-2/collagen composite. Neither DPP/collagen composite nor collagen alone conducted bone formation even at 3 weeks postimplantation. These results indicate that the bone-inducing activity of rhBMP-2 is enhanced by DPP as a co-factor of rhBMP-2 in vivo.
Saito
Takashi
T
Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 061-0293, Japan. t-saito@hoku-iryo-u.ac.jp
Kobayashi
Fumito
F
Fujii
Takeo
T
Bessho
Kazuhisa
K
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002417:Cattle; D003188:Composite Resins / Q000494:pharmacology; D003804:Dentin / Q000737:chemistry*; D004337:Drug Carriers; D004357:Drug Synergism; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D010521:Periosteum / Q000187:drug effects / Q000502:physiology; D010750:Phosphoproteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D003188:Composite Resins; D004337:Drug Carriers; D010750:Phosphoproteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C017630:phosphophoryn