Study of the development of thermoresistance in human pancreatic carcinoma cell lines using proteome analysis.
In order to find candidate proteins that are potentially associated with the thermoresistant phenotype in combination with drug resistance, we analyzed the differential protein expression in vitro in the human pancreatic cancer cell line EPP85-181-P and classical and atypical multidrug-resistant variants and their thermoresistant counterparts using proteomics. This study identifies sets of proteins that may lead to the development of thermoresistance. These results provide a fundamental basis to elucidate the molecular mechanism of thermoresistance and chemoresistance phenomena that may assist the therapy of inoperable cancers.
Poland
Julia
J
Institut für Laboratoriumsmedizin und Pathobiochemie, Universitätsklinikum Charité, Berlin, Germany.
Urbani
Andrea
A
Lage
Hermann
H
Schnölzer
Martina
M
Sinha
Pranav
P
D000220:Adaptation, Biological; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology; D010641:Phenotype; D040901:Proteomics / Q000379:methods*; D013696:Temperature
D009363:Neoplasm Proteins