Evaluation of recombination repair pathways in thermal radiosensitization.
Thermal radiosensitization has been shown to cause inhibition of repair of sublethal and potentially lethal damage and DNA DSBs. In this study we assessed thermal radiosensitization in mutants deficient in homologous recombinational (HR) repair and nonhomologous end joining repair (NHEJ). Using cells of the mouse wild-type embryo fibroblast cell line MEF and its Ku80(-/-) derivative that is deficient in NHEJ, we showed that thermal radiosensitization is the same in both cell lines. Further studies with cells of the wild-type CHO-AA8 cell line and its derivative IRS(ISF), which is deficient in HR, also showed comparable thermal radiosensitization in both cell lines. Further experiments using cells of chicken DT40 cell lines also showed comparable thermal radiosensitization between the wild-type HR mutant Rad54, the NHEJ mutant Ku70, and the double mutant Rad 54-Ku70. These results indicate that the HR and NHEJ pathways may not be targets for thermal radiosensitization.
Raaphorst
G P
GP
Medical Physics Department, Ottawa Regional Cancer Centre, Ottawa, Ontario K1H 1C4, Canada. graaphorst@orcc.on.ca
Maude-Leblanc
J
J
Li
L
L
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002645:Chickens; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000502:physiology* / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000172:deficiency*; D006358:Hot Temperature; D051379:Mice; D011836:Radiation Tolerance / Q000528:radiation effects*; D011995:Recombination, Genetic / Q000528:radiation effects*
D004268:DNA-Binding Proteins