A novel immortalized human endometrial stromal cell line with normal progestational response.
Obtaining primary human endometrial stromal cells (HESCs) for in vitro studies is limited by the scarcity of adequate human material and the inability to passage these cells in culture for long periods. Immortalization of these cells would greatly facilitate studies; however, the process of immortalization often results in abnormal karyotypes and aberrant functional characteristics. To meet this need, we have introduced telomerase into cultured HESCs to prevent the normal shortening of telomeres observed in adult somatic cells during mitosis. We have now developed and analyzed a newly immortalized HESC line that contains no clonal chromosomal structural or numerical abnormalities. In addition, when compared with the primary unpassaged parent cells, the new cell line displayed similar biochemical endpoints after treatment with ovarian steroids. Classical decidualization response to estradiol plus medroxyprogesterone acetate were seen in both morphologically, and progestin was seen to induce or regulate the expression of IGF binding protein-1, fibronectin, prolactin, tissue factor, plasminogen activator inhibitor-1, and Fas/Fas ligand. In summary, an immortalized HESC line has been developed that is karyotypically, morphologically, and phenotypically similar to the primary parent cells, and it is a powerful and consistent resource for in vitro work.
Krikun
Graciela
G
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, P.O. Box 208063, New Haven, Connecticut 06520-8063, USA. graciela.krikun@yale.edu
Mor
Gil
G
Alvero
Ayesha
A
Guller
Seth
S
Schatz
Frederick
F
Sapi
Eva
E
Rahman
Mizanur
M
Caze
Rebeca
R
Qumsiyeh
Mazin
M
Lockwood
Charles J
CJ
D002461:Cell Line, Transformed; D003656:Decidua / Q000502:physiology; D004717:Endometrium / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology; D053222:Fas Ligand Protein; D005260:Female; D005353:Fibronectins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics; D007621:Karyotyping; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D011372:Progestins / Q000494:pharmacology*; D011388:Prolactin / Q000235:genetics; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D019098:Telomerase / Q000378:metabolism; D013925:Thromboplastin / Q000235:genetics; D019014:fas Receptor / Q000235:genetics
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D005353:Fibronectins; D018970:Insulin-Like Growth Factor Binding Protein 1; D008562:Membrane Glycoproteins; D017395:Plasminogen Activator Inhibitor 1; D011372:Progestins; D019014:fas Receptor; D004958:Estradiol; D011388:Prolactin; D013925:Thromboplastin; D017258:Medroxyprogesterone Acetate; D019098:Telomerase