The insulator effect of the 5'HS4 region from the beta-globin chicken locus on the rabbit WAP gene promoter activity in transgenic mice.
Previous studies have shown that the 5'HS4 DNaseI hypersensitive site of the chicken beta-globin locus is endowed with classic insulator activities: (i) it blocks the interaction between promoter and enhancers when it is inserted between them (ii) it confers expression of integrated foreign genes independent of their position in the chromatin. The aim of this present work was to determine whether the 5'HS4 element was able to stimulate the expression level and/or to increase the expression frequency of a luc+ reporter gene controlled by the rabbit WAP gene promoter. Two constructs with 5'HS4 insulator (p5'HS4-WAPluc) or without (pWAPluc) were introduced in mouse fertilised oocytes. All transgenic lines containing the 5'HS4 element (six lines) expressed the transgene whereas only two out of eight lines harbouring the pWAP-luc construct expressed the transgene to a significant level. Moreover, the mean level of expression was seven times higher in p5'HS4WAP-luc lines than in pWAP-luc lines. Even all these benefits on transgene expression, the 5'HS4 element did not confer a copy-dependent expression, did not decrease the ectopic expression of the reporter gene and did not decrease the variability of expression. Thus, the 5'HS4 element does not have all the properties of a perfect insulator on a construct containing the luc+ reporter gene controlled by the rabbit WAP promoter.
Rival-Gervier
Sylvie
S
Unité de Biologie du Développement et Biotechnologies, Institut National de la Recherche Agronomique, F-78352 Jouyv-en-Josas, France. rival@jouy.inra.fr
Pantano
Thais
T
Viglietta
Céline
C
Maeder
Caroline
C
Prince
Sonia
S
Attal
Joe
J
Jolivet
Geneviève
G
Houdebine
Louis-Marie
LM
D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005914:Globins / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008894:Milk Proteins / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits
D008894:Milk Proteins; C024471:whey acidic proteins; D005914:Globins; D008156:Luciferases