In vivo interaction between CDKA and eIF4A: a possible mechanism linking translation and cell proliferation.
In a proteomics-based screen for proteins interacting with cyclin-dependent protein kinase (CDK), we have identified a novel CDK complex containing the eukaryotic translation initiation factor, eIF4A. Reciprocal immunoprecipitations using antibodies against eIF4A indicate that the interaction is specific. The CDKA-eIF4A complex is abundant in actively proliferating and growing cells but is absent from cells that have ceased dividing. The CDKA-eIF4A complex contains kinase activity that is sensitive to the CDK-specific inhibitor roscovitine. This interaction points to a possible molecular mechanism linking cell proliferation with translational control.
Hutchins
Andrew P
AP
Department of Cell and Developmental Biology, John Innes Centre, NR4 7UH, Norwich, UK.
Roberts
Gethin R
GR
Lloyd
Clive W
CW
Doonan
John H
JH
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology*; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D039601:Eukaryotic Initiation Factor-4A / Q000737:chemistry / Q000378:metabolism*; D011233:Precipitin Tests / Q000379:methods; D014176:Protein Biosynthesis / Q000502:physiology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D011687:Purines / Q000494:pharmacology; D000077546:Roscovitine; D014026:Nicotiana / Q000166:cytology / Q000201:enzymology
D004791:Enzyme Inhibitors; D047428:Protein Kinase Inhibitors; D011687:Purines; D000077546:Roscovitine; D011494:Protein Kinases; C102333:histone H1 kinase; D018844:Cyclin-Dependent Kinases; D039601:Eukaryotic Initiation Factor-4A