Triplex-forming oligonucleotide target sequences in the human genome.
The existence of sequences in the human genome which can be a target for triplex formation, and accordingly are candidates for anti-gene therapies, has been studied by using bioinformatics tools. It was found that the population of triplex-forming oligonucleotide target sequences (TTS) is much more abundant than that expected from simple random models. The population of TTS is large in all the genome, without major differences between chromosomes. A wide analysis along annotated regions of the genome allows us to demonstrate that the largest relative concentration of TTS is found in regulatory regions, especially in promoter zones, which suggests a tremendous potentiality for triplex strategy in the control of gene expression. The dependence of the stability and selectivity of the triplexes on the length of the TTS is also analysed using knowledge-based rules.
Goñi
J Ramon
JR
Molecular Modelling and Bioinformatics Unit, Institut de Recerca Biomédica, Parc Científic de Barcelona, Josep Samitier 1-5, Barcelona 08028, Spain.
de la Cruz
Xavier
X
Orozco
Modesto
M
D019295:Computational Biology; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011336:Probability; D011401:Promoter Regions, Genetic / Q000235:genetics
D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA