title: Expression of intercellular adhesion molecule-1 (ICAM- 1) during ischemia-reperfusion in human liver tissue allograft: image analysis by confocal laser scanning microscopy.
abstract: We studied and quantified the effect of ischemia-reperfusion on expression of intercellular adhesion molecule-1 (ICAM-1) on sinusoidal endothelial cells and hepatocyte plasma membranes by means of confocal laser scanning microscopy imaging. We found that ischemia induced an increase in ICAM-1 expression on sinusoidal endothelial cells and hepatocytes. After reperfusion, ICAM-1 expression was increased on sinusoidal endothelial cells, whereas it was unmodified on hepatocytes. On the other hand, ICAM-1 expression was not correlated to ischemia-reperfusion liver injury. Therefore, hepatocellular ischemia-reperfusion injury could be induced by other causes than immune-mediated damages.
authors.affiliation: INSERM U 559, Faculté de Médecine, 27 boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Liliane
authors.initials: L
authors.lastname: Benkoel
authors.forename: Frank
authors.initials: F
authors.lastname: Dodero
authors.forename: Jean
authors.initials: J
authors.lastname: Hardwigsen
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Benoliel
authors.forename: Pierre
authors.initials: P
authors.lastname: Bongrand
authors.forename: Danielle
authors.initials: D
authors.lastname: Botta-Fridlund
authors.forename: Yves Patrice
authors.initials: YP
authors.lastname: Le Treut
authors.forename: Albert
authors.initials: A
authors.lastname: Chamlian
authors.forename: Dominique
authors.initials: D
authors.lastname: Lombardo
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D018799:Intercellular Adhesion Molecule-1