title: Sequence, distribution and quantification of the motilin precursor in the cat.
abstract: Due to motilin's relation to the migrating motor complex (MMC), the physiology of motilin has been mostly studied in man and dog. The cat does not have an MMC pattern, and little is known about cat motilin. Therefore we identified the cat motilin precursor (GenBank accession no. AF127917) and developed a quantitative polymerase chain reaction (PCR) to explore its distribution in the gastrointestinal tract and in the central nervous system (CNS). The precursor is closely related to the dog precursor and consists of an open reading frame of 348bp encoding the signal peptide (25 amino acids), the motilin sequence (22 amino acids) and the motilin associated peptide (69 amino acids). One amino acid of the signal peptide was subject to gene polymorphism. Quantification of motilin messenger RNA (mRNA) was for the first time achieved. It is most abundant in the gastrointestinal tract, with the highest concentration in the duodenum, the lowest in the colon and is not detectable in the corpus. However an important expression was also observed in several regions of the CNS, except the striatum and cerebral cortex. The highest level was in the hypothalamus (although 23-fold lower than in the duodenum), the lowest level in the pons. Moderate levels were found in the thyroid. These data suggest that the physiological role of motilin may extend beyond its effect on gastrointestinal motility.
authors.affiliation: Center for Pathophysilogy, Gastroenterological Research, University of Leuven, Gasthuisberg ON, B-3000 Leuven, Belgium.
authors.forename: Luo
authors.initials: L
authors.lastname: Xu
authors.forename: Inge
authors.initials: I
authors.lastname: Depoortere
authors.forename: Leen
authors.initials: L
authors.lastname: Thielemans
authors.forename: Zhong
authors.initials: Z
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Tang
authors.forename: Theo L
authors.initials: TL
authors.lastname: Peeters
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002415:Cats; D041981:Gastrointestinal Tract; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D009037:Motilin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D009037:Motilin