A Dickkopf- 3-related gene is expressed in differentiating nematocytes in the basal metazoan Hydra.
In vertebrate development the Dickkopf protein family carries out multiple functions and is represented by at least four different genes with distinct biological activities. In invertebrates such as Drosophila and Caenorhabditis, Dickkopf genes have so far not been identified. Here we describe the identification and characterization of a Dickkopf gene with a deduced amino acid sequence closely related to that of chicken Dkk-3 in the basal metazoan Hydra. HyDkk-3 appears to be the only Dickkopf gene in Hydra. The gene is expressed in the gastric region in nematocytes at a late differentiation stage. In silico searches of EST and genome databases indicated the absence of Dkk genes from the protostomes Drosophila and Caenorhabditis, whereas within the deuterostomes, a Dkk-3 gene could be identified in the genome of the urochordate Ciona intestinalis. The results indicate that at an early stage of evolution of multicellularity Dickkopf proteins have already played important roles as developmental signals. They also suggest that vertebrate Dkk-1, 2 and 4 may have originated from a common ancestor gene of Dkk-3.
Fedders
Henning
H
Zoological Institute, Christian-Albrechts-University Kiel, Olshausenstrasse 40, 24098, Kiel, Germany.
Augustin
René
R
Bosch
Thomas C G
TC
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans; D018925:Chemokines; D002645:Chickens; D004330:Drosophila; D004625:Embryo, Nonmammalian / Q000196:embryology; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D006829:Hydra / Q000196:embryology / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment
D048868:Adaptor Proteins, Signal Transducing; D018925:Chemokines; C498624:DKK3 protein, human; C405061:Dkk3 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins