title: Pancolonic indigo carmine dye spraying for the detection of dysplasia in ulcerative colitis.
abstract: OBJECTIVE
  Colonoscopic surveillance for cancer in longstanding extensive ulcerative colitis relies heavily on non-targeted mucosal biopsies. Chromoendoscopy can aid detection of subtle mucosal abnormalities. We hypothesised that routine pancolonic indigo carmine dye spraying would improve the macroscopic detection of dysplasia and reduce the dependence on non-targeted biopsies.
  
  
  METHODS
  One hundred patients with longstanding extensive ulcerative colitis attending for colonoscopic surveillance underwent "back to back" colonoscopies. During the first examination, visible abnormalities were biopsied, and quadrantic non-targeted biopsies were taken every 10 cm. Pancolonic indigo carmine (0.1%) was used during the second colonoscopic examination, and any additional visible abnormalities were biopsied.
  
  
  RESULTS
  Median extubation times for the first and second colonoscopies were 11 and 10 minutes, respectively. The non-targeted biopsy protocol detected no dysplasia in 2904 biopsies. Forty three mucosal abnormalities (20 patients) were detected during the pre-dye spray colonoscopy of which two (two patients) were dysplastic: both were considered to be dysplasia associated lesions/masses. A total of 114 additional abnormalities (55 patients) were detected following dye spraying, of which seven (five patients) were dysplastic: all were considered to be adenomas. There was a strong trend towards statistically increased dysplasia detection following dye spraying (p = 0.06, paired exact test). The targeted biopsy protocol detected dysplasia in significantly more patients than the non-targeted protocol (p = 0.02, paired exact test).
  
  
  CONCLUSIONS
  No dysplasia was detected in 2904 non-targeted biopsies. In comparison, a targeted biopsy protocol with pancolonic chromoendoscopy required fewer biopsies (157) yet detected nine dysplastic lesions, seven of which were only visible after indigo carmine application. Careful mucosal examination aided by pancolonic chromoendoscopy and targeted biopsies of suspicious lesions may be a more effective surveillance methodology than taking multiple non-targeted biopsies.
authors.affiliation: Wolfson Unit for Endoscopy and Department of Gastroenterology, St Mark's Hospital, Watford Road, Harrow, Middlesex HA1 3UJ, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Rutter
authors.forename: B P
authors.initials: BP
authors.lastname: Saunders
authors.forename: G
authors.initials: G
authors.lastname: Schofield
authors.forename: A
authors.initials: A
authors.lastname: Forbes
authors.forename: A B
authors.initials: AB
authors.lastname: Price
authors.forename: I C
authors.initials: IC
authors.lastname: Talbot
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D003093:Colitis, Ulcerative / Q000473:pathology*; D003106:Colon / Q000473:pathology*; D003113:Colonoscopy; D004396:Coloring Agents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007203:Indigo Carmine; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D004396:Coloring Agents; D007203:Indigo Carmine