title: Neonatal-mouse infectivity of intact Cryptosporidium parvum oocysts isolated after optimized in vitro excystation.
abstract: We reexamined the finding of Neumann et al. that intact Cryptosporidium parvum oocysts obtained after in vitro excystation were infectious for neonatal CD-1 mice. We used both established excystation protocols and our own protocol that maximized excystation. Although intact oocysts isolated after any of three protocols were infectious for neonatal CD-1 mice, the infectivity of intact oocysts isolated with our optimized excystation protocol was significantly lower than the infectivity of intact oocysts isolated after established protocols or from fresh oocysts. Excystation should not be considered a valid measure of C. parvum viability, given that it is biologically implausible for oocysts to be nonviable and yet infectious.
authors.affiliation: Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California-Davis, Tulare, California 93274, USA.
authors.forename: L
authors.initials: L
authors.lastname: Hou
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Dunbar
authors.forename: R
authors.initials: R
authors.lastname: Moeller
authors.forename: B
authors.initials: B
authors.lastname: Palermo
authors.forename: E R
authors.initials: ER
authors.lastname: Atwill
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D003457:Cryptosporidiosis / Q000469:parasitology / Q000473:pathology; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D008856:Microscopy, Fluorescence; D033661:Oocysts / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity*