The dendritic cell receptor DC-SIGN discriminates among species and life cycle forms of Leishmania.
Infection of dendritic cells by the human protozoal parasite Leishmania is part of its survival strategy. The dendritic cell receptors for Leishmania have not been established and might differ in their interactions among Leishmania species and infective stages. We present evidence that the surface C-type lectin DC-SIGN (CD 209) is a receptor for promastigote and amastigote infective stages from both visceral (Leishmania infantum) and New World cutaneous (Leishmania pifanoi) Leishmania species, but not for Leishmania major metacyclic promastigotes, an Old World species causing cutaneous leishmaniasis. Leishmania binding to DC-SIGN was found to be independent of lipophosphoglycan, the major glycoconjugate of the promastigote plasma membrane. Our findings emphasize the relevance of DC-SIGN in Leishmania-dendritic cell interactions, an essential link between innate and Leishmania-specific adaptive immune responses, and suggest that DC-SIGN might be a therapeutic target for both visceral and cutaneous leishmaniasis
Colmenares
María
M
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Corbí
Angel L
AL
Turco
Salvatore J
SJ
Rivas
Luis
L
D000818:Animals; D001665:Binding Sites / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology*; D006028:Glycosphingolipids / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D037181:Lectins, C-Type / Q000378:metabolism / Q000502:physiology*; D018314:Leishmania infantum / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism; D018320:Leishmania major / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology; D009895:Opsonin Proteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism / Q000502:physiology*; D011971:Receptors, Immunologic / Q000378:metabolism; D013045:Species Specificity; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D006028:Glycosphingolipids; D037181:Lectins, C-Type; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C008290:lipophosphonoglycan