title: Inositol 1,4,5-triphosphate receptor protein immunohistochemistry of cerebellar Purkinje cells in two dogs with hypoglycemia.
abstract: Immunohistochemical study was performed on cerebellar Purkinje cells of two dogs with hypoglycemia using an antibody against the inositol 1,4,5-triphosphate receptor that is identical to the cerebellar Purkinje cell glycoprotein P(400) (P(400)/InsP(3)R). In the cerebellar neocortex of an acute case of hypoglycemia, the P(400)/InsP(3)R staining of hypoglycemic Purkinje cells was heterogeneous: some peripheral dendrites, including spiny branchlets, were negative and others were stained with various intensities, although Purkinje cells were morphologically intact by hematoxylin and eosin (HE) stain. In a chronic case of hypoglycemia, almost all the dendrites of Purkinje cells of both the neo- and archicortex of the cerebellum were not stained with the P(400)/InsP(3)R antibody. This is in contrast to the normal dog where Purkinje cell bodies, axons, and dendrites, including spiny branchlets, are intensely stained by the P(400)/InsP(3)R antibody. These results suggest that P(400)/InsP(3)R immunolabeling of Purkinje cells decreased, despite their morphology being preserved by HE stain, and that the function of P(400)/InsP(3)R, especially in spiny branchlets that receive inputs originating from axon terminals of parallel fibers, may be impaired in hypoglycemia.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami 4-101, Koyamacho, Tottorishi, Tottori 680-8553, Japan. morita@muses.tottori-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Sawada
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Miyata
authors.forename: E
authors.initials: E
authors.lastname: Ohama
mesh_terms: D027322:Amino Acid Transport System X-AG / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015220:Calcium Channels / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology*; D004285:Dogs; D007003:Hypoglycemia / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors; D011689:Purkinje Cells / Q000276:immunology / Q000473:pathology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology*
chemical_list: D027322:Amino Acid Transport System X-AG; D000906:Antibodies; D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear