5-Aminolevulinic acid-induced fluorescence in bronchial tumours: dependency on the patterns of tumour invasion.
5-ALA-induced protoporphyrin IX (PPIX) fluorescence kinetics was quantified by fluorescence microscopy in three-dimensional organ co-cultures of human bronchial epithelium, which were infiltrated by four different lung tumour cell lines (EPLC-M31, LCLC-103H, NCI-H125 and NCI-H841). Corresponding fluorescence measurements were performed in monolayer cultures of these tumour cell lines and BEAS-2B cells as a model for normal bronchial epithelium by flow cytometry. Significant differences of fluorescence intensities (FI) between the tumours were detected in organ co-cultures as well as in single cell measurements. Relative FI values in organ co-cultures (FI(EPLC-32M1)>FI(LCLC-H103)>FI(NCI-H125)>FI(NCI-H841)) did not correspond to the measurements in single cells (FI(LCLC-H103)>FI(NCI-H125)>FI(NCI-H841)>FI(EPLC-32M1)). Histology of organ co-cultures revealed different patterns of invasion and tumour cell densities depending on the tumour type. After correction of FI in the co-cultures to tumour cell density the correlation coefficient for fluorescence values between both models increased considerably. Thus, additionally to distinctive features of 5-ALA metabolism, patterns of tumour invasion may be a factor determining 5-ALA-induced fluorescence. Considering these results, a pronounced heterogeneity of 5-ALA-induced fluorescence might be expected in different bronchial tumours in vivo. This could interfere with the diagnostic reliability of 5-ALA-induced fluorescence for early tumour detection.
Gamarra
F
F
Medizinische Klinik--Innenstadt, Klinikum der Ludwig-Maximilians-Universität, Ziemssenstr. 1, 80336 München, Germany.
Lingk
P
P
Marmarova
A
A
Edelmann
M
M
Hautmann
H
H
Stepp
H
H
Baumgartner
R
R
Huber
R M
RM
D000622:Aminolevulinic Acid / Q000737:chemistry*; D001984:Bronchial Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000473:pathology; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology; D009361:Neoplasm Invasiveness; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*
D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid