title: Susceptibility testing of sequential isolates of Aspergillus fumigatus recovered from treated patients.
abstract: Two-hundred sequential Aspergillus fumigatus isolates recovered from 26 immunocompromised patients with invasive aspergillosis or bronchial colonization were tested for their in vitro susceptibility to posaconazole, itraconazole, voriconazole, terbinafine and amphotericin B. Twenty-one patients were treated with amphotericin B and/or itraconazole. Antifungal susceptibilities of the isolates recovered before treatment were not significantly different from those of isolates recovered after the onset of antifungal therapy. The highest MICs were 0.125, 0.5, 0.5, 1 and 1 microg ml(-1) for posaconazole, itraconazole, voriconazole, terbinafine and amphotericin B, respectively. It is concluded that the emergence of resistance in A. fumigatus during antifungal therapy with amphotericin B or itraconazole is an uncommon phenomenon.
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Eric
authors.initials: E
authors.lastname: Dannaoui
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Joseph
authors.initials: J
authors.lastname: Meletiadis
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Tortorano
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Françoise
authors.initials: F
authors.lastname: Symoens
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Nicole
authors.initials: N
authors.lastname: Nolard
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Maria-Anna
authors.initials: MA
authors.lastname: Viviani
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Marie-Antoinette
authors.initials: MA
authors.lastname: Piens
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Lebeau
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Verweij
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.forename: Renée
authors.initials: R
authors.lastname: Grillot
authors.affiliation: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, 69373 Lyon Cedex 08, France 2Department of Medical Microbiology, University Medical Center St Radboud, 6500 HB Nijmegen, The Netherlands 3Istituto di Igiene e Medicina Preventiva, Università degli Studi -IRCCS Ospedale Maggiore, 20122 Milan, Italy 4Scientific Institute of Public Health, B-1050 Brussels, Belgium 5Laboratoire Interactions Cellulaires Parasite-Hôte, Université Joseph Fourier Grenoble I, 38706 Grenoble Cedex, France 6European Research Group on Biotypes and Genotypes of Aspergillus fumigatus.
authors.lastname: And The Ebga Network
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology / Q000627:therapeutic use*; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001228:Aspergillosis / Q000188:drug therapy* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000187:drug effects*; D025141:Drug Resistance, Fungal; D006801:Humans; D016867:Immunocompromised Host; D017964:Itraconazole / Q000494:pharmacology / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B