title: [Crosslinking bovine pericardial bioprosthetic heart valves by phthalocyanine sensitized photooxidation reaction].
abstract: OBJECTIVE
  To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.
  
  
  METHODS
  Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.
  
  
  RESULTS
  Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.
  
  
  CONCLUSIONS
  Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Ying-wu
authors.initials: YW
authors.lastname: Tang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liang
authors.forename: Jian-ye
authors.initials: JY
authors.lastname: Zhou
authors.forename: Jing-wei
authors.initials: JW
authors.lastname: Cui
authors.forename: Sheng-shou
authors.initials: SS
authors.lastname: Hu
authors.forename: Fu-shi
authors.initials: FS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006350:Heart Valve Prosthesis; D007211:Indoles; D054833:Isoindoles; D010496:Pericardium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003432:Cross-Linking Reagents; D007211:Indoles; D054833:Isoindoles; C013647:phthalocyanine