title: Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system.
abstract: HOX genes are well known as master control genes in embryonic morphogenesis. We hypothesized that HOX genes give cells spatial information to maintain tissue- or organ-specificity in adult body and that the deregulated expression of HOX genes results in tumor development. We established a comprehensive analysis system to quantify expression of 39 human HOX genes based on the real-time reverse transcription PCR (RT-PCR) method. Analysis of 39 HOX genes of 20 normal adult organs by this system revealed that 5' HOX genes were expressed in organs in the caudal parts of the body, and that the more caudal regions the more numbers of HOX genes were expressed. It was also found that the expression patterns of HOX genes were more similar in the adjacent genes on the same cluster rather than in those belonging to the same paralogs. Compared with normal thyroid tissues, thyroid cancer cell lines showed the altered expression of some HOX genes, especially Abd-B homeobox family genes. Our results showed that HOX genes were organ-specifically expressed in adult body and that the deregulated expressions of Abd-B family genes were implicated in thyroid tumor development.
authors.affiliation: Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hamada
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Murakawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Takada
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Tada
authors.forename: Ikuko
authors.initials: I
authors.lastname: Nogami
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Hayashi
authors.forename: Shoji
authors.initials: S
authors.lastname: Nakamori
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katoh
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Moriuchi
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003199:Computer Systems; D015870:Gene Expression; D020869:Gene Expression Profiling; D005801:Genes, Homeobox; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D000199:Actins; D012333:RNA, Messenger; D005993:Glycerolphosphate Dehydrogenase