[Conditions of contrast ultrasound that affect the proliferation of rat smooth muscle cells].
OBJECTIVE
To investigate the effect of an echo-contrast agent on the proliferation of rat smooth muscle cells under different ultrasound conditions.


METHODS
The vascular smooth muscle cells of rats were cultured with echo-contrast agent in 96-well plates, followed by exposure to ultrasound of different conditions. Trypan blue staining was performed 48 h later, and the proliferation of the cells observed by MTT assay.


RESULTS
No cytotoxicity was found by trypan blue staining when the mechanical index of ultrasound was below 0.75. Compared with the control cells, the proliferation of the smooth muscle cells was decreased following the exposure as the mechanical index of ultrasound increased. The most obvious inhibition of cell proliferation was resulted when the microbubble concentration was 20% for a 60-second exposure.


CONCLUSIONS
The inhibition of the vascular smooth muscle cell proliferation by echo-contrast agent destruction is correlated with the mechanical index of the ultrasound, concentration of the echo-contrast agent, and exposure time of ultrasound.
Xiu
Jian-cheng
JC
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. xiujc@fimmu.com
Ou
Wen-chao
WC
Xie
Jin-guo
JG
Yang
Li
L
Bin
Jian-ping
JP
Zha
Dao-gang
DG
Liu
Yi-li
YL
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003287:Contrast Media / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014465:Ultrasonics
D003287:Contrast Media