[Study of blue light induced DNA damage of retinal pigment epithelium(RPE) cells and the protection of vitamin C].
To evaluate protection of vitamin C on blue light-induced DNA damage of human retinal pigment epithelium (RPE) cells. The cultured RPE cells were divided into 3 groups: Control group (no blue light exposure), blue light exposure group (blue light exposure for 20 minutes) and blue light exposure + vitamin C group (blue light exposure + 100 mumol/L vitamin C). Travigen's comet assay kit and Euclid comet assay software were used to assay the DNA damage levels. The DNA percentage in the tail of electrophoretogram in the three groups were 18.44%, 54.42% and 32.43% respectively (p < 0.01). Tail moments were 8.2, 48.3, and 18.4 respectively (p < 0.01). Blue light could induce DNA damage to RPE cells but vitamin C could protect the RPE cells from the blue light-induced DNA damage.
Zhou
Jian Wei
JW
Second Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310009. zj219@columbia.edu
Ren
Guo Liang
GL
Zhang
Xiao Ming
XM
Zhu
Xi
X
Lin
Hai Yan
HY
Zhou
Ji Lin
JL
D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D008027:Light / Q000009:adverse effects*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D020011:Protective Agents / Q000494:pharmacology*
D020011:Protective Agents; D001205:Ascorbic Acid