title: Filamentous marine fungi as producers of O-glycosylhydrolases: beta-1,3-glucanase from Chaetomium indicum.
abstract: Ninety fungal strains (42 species) isolated from marine habitats were studied for their ability to produce extracellular enzymes. Cultural filtrates of these strains were shown to contain a series of glycosidases (beta-glucosidases, N-acetyl-beta-glucosaminidases, beta-galactosidases alpha-mannosidases) and glucanases (1,3-beta-glucanases, amylases) which varied with habitat. The level of activity depended on the species of fungi. Several promising strains capable of producing both individual enzymes and a set of enzymes for splitting carbohydrate-containing compound have been isolated. Optimal conditions for growth of Chaetomium indicum and for biosynthesis of beta-1,3-glucanase were determined. beta-1,3-Glucanase was isolated using ion-exchange chromatography, ultrafiltration, and gel filtration. The presence of 2 enzyme forms was shown; both forms were exo-beta-1,3-glucanases.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022, Vladivostok-22, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Burtseva
authors.forename: N S
authors.initials: NS
authors.lastname: Verigina
authors.forename: V V
authors.initials: VV
authors.lastname: Sova
authors.forename: M V
authors.initials: MV
authors.lastname: Pivkin
authors.forename: T N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D002597:Chaetomium / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005843:Geography; D043326:Glucan 1,3-beta-Glucosidase / Q000096:biosynthesis* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010140:Pacific Ocean; D013379:Substrate Specificity; D013696:Temperature; D014462:Ultrafiltration
chemical_list: D043326:Glucan 1,3-beta-Glucosidase