Effect of carnosine administration on the immune response of rabbit to Schistosoma mansoni antigens.
SDS-PAGE separation of soluble worm antigen preparation (SWAP), cercarial antigen preparation (CAP), and soluble egg antigen (SEA) of Schistosoma mansoni showed obvious qualitative and quantitative differences. The shared polypeptides of the three stages of S. mansoni were 116, 72.768 and 32.367 kDa under reducing conditions. The different anti-sera raised in rabbits against the different stages of antigens were recognized by electroimmune transfer blotting (EITB). Each of the 3 groups separated eight bands. Carnosine treatment of rabbits immunized with SWAP, CAP or SEA resulted in the disappearance of two bands in SWAP group and one band in CAP group in comparison with the non-treated immunized groups. This indicated that the carnosine modulated immune response of rabbits against S. mansoni antigens.
Soliman
Kawther
K
Department of Biochemistry, Faculty of Medicine, Cairo University, Egypt. solimank2002@yahoo.com
Abou-El Dobal
Sanaa
S
Marei
Naema
N
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000187:drug effects; D000947:Antigens, Helminth / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D002336:Carnosine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D012550:Schistosoma mansoni / Q000187:drug effects / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D002336:Carnosine