Aedes albopictus is a natural vector of Dirofilaria immitis in Italy.
Investigations were carried out in Padova town (Veneto region, NE Italy) to define the actual role of Aedes albopictus in the natural transmission of Dirofilaria nematodes, and to assess the risk that its presence might represent for veterinary and medical health. During summer 2000-2002 daytime captures of human-attracted mosquitoes were carried out in three areas of the town. The presence of filarial parasites in mosquitoes was evaluated by PCR, and sequencing confirmed species assessment. DNA extraction was performed separately on pools of the insect abdomen and thorax-head, to discriminate between Dirofilaria infected/infective specimens. A total of 2721 mosquitoes were caught and A. albopictus was the most abundant species (2534). Filarial DNA was found in 27.5% (19/69) of the abdomen pools formed with mosquitoes collected in summer 2000, and in 11.1% (16/144) and 4.9% (6/123) thorax-head pools coming from samplings 2001 and 2002, respectively. Filarial DNA was belonging to D. immitis and all studied areas harboured infective specimens. These results prove A. albopictus as natural vector of D. immitis in Italy. Moreover, they support the hypothesis that the presence of the mosquito could affect the transmission pattern of canine heartworm disease in urban environment and, considering the aggressive anthropophylic behaviour of the species (30-48 bites/h) proven in Padova town, could enhance the circulation of filarial nematodes from animals to humans.
Cancrini
G
G
Dipartimento di Scienze di Sanità Pubblica, Sez. Parassitologia, Università "La Sapienza", P. le Aldo Moro 2, 00185 Rome, Italy. gabriella.cancrini@uniromal.it
Frangipane di Regalbono
A
A
Ricci
I
I
Tessarin
C
C
Gabrielli
S
S
Pietrobelli
M
M
D000330:Aedes / Q000469:parasitology*; D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004183:Dirofilaria immitis / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000469:parasitology / Q000635:transmission*; D005260:Female; D006801:Humans; D007299:Insect Bites and Stings / Q000469:parasitology; D007303:Insect Vectors / Q000469:parasitology*; D007558:Italy; D016133:Polymerase Chain Reaction; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
D018509:DNA, Helminth; D012341:RNA, Ribosomal, 5S