title: Assignment of Neisseria meningitidis serogroups A, C, W135, and Y anticapsular total immunoglobulin G (IgG), IgG1, and IgG2 concentrations to reference sera.
abstract: Meningococcal serogroup-specific immunoglobulin G (IgG), IgG1, and IgG2 concentrations were assigned to three reference sera, CDC 1992, 89-SF, and 96/562, for meningococcal serogroups A, C, Y, and W135 via the method of cross standardization. The sum of the serogroup-specific IgG1 and IgG2 concentrations determined for the four meningococcal serogroups showed good agreement with the serogroup-specific IgG either determined here or as previously represented. Following the assignment of meningococcal serogroup-specific IgG1 and IgG2 concentration to these reference sera, a meningococcal serogroup-specific IgG1 and IgG2 enzyme-linked immunosorbent assay protocol was developed. The serogroup A and C specific subclass distribution of a panel of adult sera collected following vaccination with any combination of meningococcal serogroup C conjugate, bivalent, or tetravalent polysaccharide vaccines was determined. For the determination of serogroup W135 and Y specific subclass distribution, an adolescent panel 28 days following a single dose of either tetravalent polysaccharide or conjugate vaccine was used. The sum of the serogroup-specific IgG1 and IgG2 showed strong correlation with the serogroup-specific total IgG determined. The assignment here of IgG1 and IgG2 subclasses to these reference sera will allow more detailed evaluation of meningococcal conjugate and polysaccharide vaccines.
authors.affiliation: Vaccine Evaluation Department, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester M13 9WZ, United Kingdom. helen.joseph@hpa.org.uk
authors.forename: Helen
authors.initials: H
authors.lastname: Joseph
authors.forename: Paul
authors.initials: P
authors.lastname: Balmer
authors.forename: Mike
authors.initials: M
authors.lastname: Bybel
authors.forename: Thomas
authors.initials: T
authors.lastname: Papa
authors.forename: Robert
authors.initials: R
authors.lastname: Ryall
authors.forename: Ray
authors.initials: R
authors.lastname: Borrow
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008589:Meningococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000276:immunology; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology*; D038381:Neisseria meningitidis, Serogroup A / Q000145:classification / Q000276:immunology; D038542:Neisseria meningitidis, Serogroup C / Q000145:classification / Q000276:immunology; D038582:Neisseria meningitidis, Serogroup W-135 / Q000145:classification / Q000276:immunology; D038581:Neisseria meningitidis, Serogroup Y / Q000145:classification / Q000276:immunology; D012015:Reference Standards; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines