[The relationship between the peripheral blood of CD61, CD63, PAC-1 and the transplant kidney function].
OBJECTIVE
To explore the relationships between the peripheral blood levels of CD61, CD63, PAC-1 and the incidence of acute rejection and tubular necrosis after renal transplantation, and recovery of the graft function.


METHODS
The peripheral blood levels of CD61, CD63, and PAC-1 of 86 patients with uremia in different stages before and after transplantations were analyzed by flow cytometry. The patients were divided into three groups: (1) twenty-nine patients with normal grafts function, (2) hirty with acute rejection and (3) twenty-seven with acute tubular necrosis. The patients with acute rejection were randomly divided into treatment group with anticoagulants and cntrol group.


RESULTS
The peripheral blood levels of CD61, CD63 and PAC-1 significantly increased (P < 0.05) in the patients with acute rejection, in comparison with those with normal grafts function and those with acute tubular necrosis. The peripheral blood levels of CD61, CD63 and PAC-1 in patients with acute rejection in anticoagulants therapy was lower, recovery time of the grafts function was shorter, one-year survival rates of patients and grafts were higher, as compared with those of controls.


CONCLUSIONS
The patients with acute rejection have significantly high peripheral blood levels of CD61, CD63 and PAC-1 before transplantation, however, these values in patients with acute tubular necrosis are not high, this suggesting that acute rejection might relate to platelet activation, while acute tubular necrosis might not relate to it. After anticoagulants therapy in patients with acute rejection, the grafts function might recover faster and their one-year survival rates and grafts might be higher in those with CD61, CD63 and PAC-1 decreasing remarkably.
Zhang
Yong
Y
Department of Urology, Chaoyang Hospital Beijing, Beijing 100020, China.
Guan
De-lin
DL
Xia
Cheng-qing
CQ
Han
Zhi-you
ZY
Xu
Jian-jun
JJ
Gao
Ju-zhong
JZ
Wu
Ke-rang
KR
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood*; D054641:Dual Specificity Phosphatase 2; D005260:Female; D006084:Graft Rejection; D006801:Humans; D039661:Integrin beta3 / Q000097:blood*; D007668:Kidney / Q000503:physiopathology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins; D054648:Protein Phosphatase 2; D017027:Protein Tyrosine Phosphatases / Q000097:blood*; D060149:Tetraspanin 30
D015703:Antigens, CD; C554586:CD63 protein, human; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D054648:Protein Phosphatase 2; C517032:DUSP2 protein, human; D054641:Dual Specificity Phosphatase 2; D017027:Protein Tyrosine Phosphatases