title: Androgen suppresses PML protein expression in prostate cancer CWR22R cells.
abstract: The ability of PML to modulate key suppressive pathways in tumor cells suggests that PML may act as a tumor suppressor. The detailed mechanism of how PML functions in prostate cancer progression, however, remains unknown. Here we demonstrate that in the presence of androgen, PML protein expression can be suppressed in CWR22R prostate cancer cells. Further studies reveal that PML can selectively suppress AR transactivation and PML protein expression positively correlates with increased p21 protein level and enhances p53 transcription ability in CWR22R cells. We also found that PML strongly inhibits CWR22R cell colony formation, while PML siRNA enhances AR activity and CWR22R cell colony formation. Together our results suggest that PML may suppress prostate cancer cell growth by inhibiting AR transactivation and/or enhancing p53 activity.
authors.affiliation: George Whipple Lab for Cancer Research, Department of Pathology, The Cancer Center, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Shauh-Der
authors.initials: SD
authors.lastname: Yeh
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Xie
authors.forename: Saleh
authors.initials: S
authors.lastname: Altuwaijri
authors.forename: Jing
authors.initials: J
authors.lastname: Ni
authors.forename: Yueh-Chiang
authors.initials: YC
authors.lastname: Hu
authors.forename: Yen-Ta
authors.initials: YT
authors.lastname: Chen
authors.forename: Bo-Ying
authors.initials: BY
authors.lastname: Bao
authors.forename: Ching-Hua
authors.initials: CH
authors.lastname: Su
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
mesh_terms: D000728:Androgens; D002455:Cell Division; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000378:metabolism*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014157:Transcription Factors / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
chemical_list: D000728:Androgens; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C070576:PML protein, human