Localization of the 31-amino-acid endothelin-1 in hamster tissue.
Endothelin (ET)-1(1-31) is a novel vasoconstrictor peptide produced by human mast cell chymase, which selectively cleaves big ET-1 at the Try(31)-Gly(32) bond. We investigated the localization of ET-1(1-31) in various hamster tissues by immunohistochemistry and compared it to the distribution of ET-1(1-21). We found that the localization and amount of ET-1(1-31) were different from those of ET-1(1-21) in each tissue. ET-1(1-31)-like immunoreactivities (IR) in the heart, lung, and adrenal gland were observed in the same areas as ET-1(1-21) but were significantly weaker, suggesting that ET-1(1-31) might play a role only in mast cell/chymase-related pathological conditions in these tissues. In the liver, ET-1(1-31)-like IR was strongly detected in Kupffer cells where ET-1(1-21)-like IR was seen more weakly. In the kidney, ET-1(1-31)-like IR was slightly higher than ET-1(1-21). These results suggest that ET-1(1-31) might have physiological roles distinct from those of ET-1(1-21) in some hamster tissues.
Kakui
Sae
S
Department of Nutrition, School of Medicine, Tokushima University, Tokushima, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan
Mawatari
Kazuaki
K
Ohnishi
Takamasa
T
Niwa
Yasuharu
Y
Tanoue
Naomi
N
Harada
Nagakatsu
N
Takahashi
Akira
A
Izumi
Keisuke
K
Nakaya
Yutaka
Y
D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D053818:Chymases; D006224:Cricetinae; D019332:Endothelin-1 / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism*; D007120:Immunochemistry; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D014018:Tissue Distribution
D019332:Endothelin-1; D010446:Peptide Fragments; D010455:Peptides; C114932:endothelin-1 (1-31); D012697:Serine Endopeptidases; D053818:Chymases