[Examination of sex-hormonal activity of some additives for PVDC film].
Stabilizers (epoxidized linseed oil and epoxidized soybean oil) and plasticizers (acetyl tributyl citrate, diacetyl monolauryl glyceride and dibutyl sebacate) commonly used in polyvinylidene chloride (PVDC) films and extracts of such films were investigated for estrogenic and androgenic activity by means of estrogen receptor (ER) and androgen receptor (AR) competitive ligand-binding assays. Further, in in vivo experiments, ovariectomized Sprague-Dawley rats were observed for uterine wet weight change, uterine endometrium hyperplasia and vaginal mucosa cornification, following administration of each test compound or extract orally (0.5 or 500 mg/kg) or subcutaneously (0.5 or 100 mg/kg). No significant response or change was observed with any of the test compounds or extracts, either in vitro or in vivo. The results thus indicate that neither the stabilizers and plasticizers used in PVDC films, nor their extracts, exert sex-hormonal activity.
Ohta
Minoru
M
Japan Hygienic Association of Vinylidene Chloride: 1-14-7, Nishi-shimbashi, Minato-ku, Tokyo 105-0003, Japan.
Oshima
Shozo
S
Iwasa
Toshio
T
Ito
Naofumi
N
Morii
Masashi
M
Morino
Masayoshi
M
Nakamura
Tadashi
T
Nagai
Kenji
K
D000728:Androgens / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D001667:Binding, Competitive; D002951:Citrates / Q000494:pharmacology; D003998:Dicarboxylic Acids / Q000494:pharmacology; D004967:Estrogens / Q000032:analysis / Q000494:pharmacology*; D005079:Excipients / Q000032:analysis / Q000494:pharmacology*; D005260:Female; D010968:Plasticizers / Q000032:analysis / Q000494:pharmacology*; D011143:Polyvinyl Chloride / Q000031:analogs & derivatives* / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D014599:Uterus / Q000187:drug effects
D000728:Androgens; D002951:Citrates; D003998:Dicarboxylic Acids; D004967:Estrogens; D005079:Excipients; D010968:Plasticizers; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; C014953:2-acetyltributylcitrate; C055481:dibutyl sebacate; C029547:polyvinylidene chloride; D011143:Polyvinyl Chloride