Coordination of meiotic recombination, pairing, and synapsis by PHS1.
Pairing, synapsis, and recombination are prerequisites for accurate chromosome segregation in meiosis. The phs1 gene in maize is required for pairing to occur between homologous chromosomes. In the phs1 mutant, homologous chromosome synapsis is completely replaced by synapsis between nonhomologous partners. The phs1 gene is also required for installation of the meiotic recombination machinery on chromosomes, as the mutant almost completely lacks chromosomal foci of the recombination protein RAD51. Thus, in the phs1 mutant, synapsis is uncoupled from recombination and pairing. The protein encoded by the phs1 gene likely acts in a multistep process to coordinate pairing, recombination, and synapsis.
Pawlowski
Wojciech P
WP
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. wpawlows@nature.berkeley.edu
Golubovskaya
Inna N
IN
Timofejeva
Ljudmilla
L
Meeley
Robert B
RB
Sheridan
William F
WF
Cande
W Zacheus
WZ
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D023902:Chromosome Pairing; D032461:Chromosomes, Plant / Q000502:physiology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018744:DNA, Plant / Q000378:metabolism; D004268:DNA-Binding Proteins; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling / Q000379:methods; D008540:Meiosis; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D016415:Sequence Alignment; D013573:Synaptonemal Complex / Q000378:metabolism / Q000648:ultrastructure; D016615:Telomere / Q000502:physiology; D003313:Zea mays / Q000235:genetics* / Q000502:physiology
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012341:RNA, Ribosomal, 5S; D051135:Rad51 Recombinase