Utilization of aminoaromatic acids by a methanogenic enrichment culture and by a novel Citrobacter freundii strain.
Following incubation of mesophilic methanogenic floccular sludge from a lab-scale upflow anaerobic sludge bed reactor used to treat cattle manure wastewater, a stable 5-aminosalicylate-degrading enrichment culture was obtained. Subsequently, a Citrobacter freundii strain, WA1, was isolated from the 5-aminosalicylate-degrading methanogenic consortium. The methanogenic enrichment culture degraded 5-aminosalicylate completely to CH4, CO2 and NH4+, while C. freundii strain WA1 reduced 5-aminosalicylate with simultaneous deamination to 2-hydroxybenzyl alcohol during anaerobic growth with electron donors such as pyruvate, glucose or serine. When grown on pyruvate, C. freundii WA1 converted 3-aminobenzoate to benzyl alcohol and also reduced benzaldehyde to benzyl alcohol. Pyruvate was fermented to acetate, CO2, H2 and small amounts of lactate, succinate and formate. Less lactate (30%) was produced from pyruvate when C. freundii WA1 grew with 5-aminosalicylate as co-substrate.
Savelieva
Olga
O
Department of Microbiology, Moscow State University, 119899 Moscow, Russia. osavelieva@hotbox.ru
Kotova
Irina
I
Roelofsen
Wim
W
Stams
Alfons J M
AJ
Netrusov
Alexander
A
D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D001547:Benzaldehydes / Q000378:metabolism; D019905:Benzyl Alcohol / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D016971:Citrobacter freundii / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D007700:Kinetics; D019804:Mesalamine / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D008969:Molecular Sequence Data; D012694:Serine / Q000378:metabolism; D012722:Sewage / Q000382:microbiology
D001547:Benzaldehydes; D002264:Carboxylic Acids; D004275:DNA, Ribosomal; D006841:Hydrocarbons, Aromatic; D012722:Sewage; D002245:Carbon Dioxide; D012694:Serine; D019804:Mesalamine; D000641:Ammonia; D006859:Hydrogen; D005947:Glucose; D019905:Benzyl Alcohol; D008697:Methane; C032175:benzaldehyde