Standard and digestive metabolism in the banded water snake, Nerodia fasciata fasciata.
Estimating energy costs by respirometry is fundamental to many studies of the ecology, behavior and evolution of reptiles. However, traditional respirometry procedures seldom incorporate objective techniques for removal of outliers from estimates of metabolic parameters. We demonstrate how computer-automated respirometry equipment, which records many respiratory measurements over short intervals, can be coupled with mathematical procedures to produce robust estimates of pre- and post-prandial metabolism in banded water snakes (Nerodia fasciata fasciata). Standard metabolic rate of N. f. fasciata was estimated to be 1.21 ml O2/h (mass = 30.21 +/- 0.74 g) at 25 degrees C. After ingestion of a fish equaling 20% of their body mass, snakes exhibited a fivefold increase in metabolic rate with peak O2 consumption rate (VO2) reaching 6.5 ml O2/h. Total cost of digestion was 5.44 kJ, equivalent to approximately 21% of the energy in the meal. Repeated measurements of metabolism in the same individuals revealed that our methods yielded similar results, even when individuals exhibited different patterns of VO2 variation between respiratory trials. Our results underscore the importance of obtaining many VO2 measurements, coupled with objective removal of outlier values from estimates of metabolic rate, especially when metabolic values are to be interpreted in a comparative context.
Hopkins
W A
WA
Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA. hopkins@srel.edu
Roe
J H
JH
Philippi
T
T
Congdon
J D
JD
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology; D017812:Colubridae / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D004734:Energy Metabolism; D010101:Oxygen Consumption; D019518:Postprandial Period; D013997:Time Factors