Establishment of an enzyme-linked immunosorbent assay for measurement of sotalol.
We have established an enzyme-linked immunosorbent assay suitable for routine monitoring of serum levels of sotalol. Anti-sotalol antibody was obtained by immunizing rabbits with sotalol conjugated with bovine serum albumin using the N-succinimidyl ester method. An enzyme marker was similarly prepared by coupling sotalol with beta-D-galactosidase. The detection limit of sotalol by the enzyme-linked immunosorbent assay was approximately 32 ng/ml with 50-microl samples. This assay was specific for sotalol because of very slight cross-reactivity with 4-(methanesulfonylamino)benzonitrile (1.6%), but none with D,L-isoproterenol. Using this assay, drug levels were easily measured in the serum of rabbits after oral administration of sotalol at a single dose of 3 mg/kg. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of sotalol.
Saita
Tetsuya
T
Faculty of Hospital Pharmacy, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan. saita@post.saga-med.ac.jp
Fujito
Hiroshi
H
Nakano
Yukitaka
Y
Mori
Masato
M
D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D000918:Antibody Specificity; D001711:Biotransformation; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D008297:Male; D011817:Rabbits; D013015:Sotalol / Q000032:analysis* / Q000493:pharmacokinetics; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000737:chemistry
D000319:Adrenergic beta-Antagonists; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D013015:Sotalol; D001616:beta-Galactosidase