[Suppression of nitric oxide on cytotoxicity of Lomustine in glioma cell line BT- 325].
To investigate the effect of nitric oxide (NO) on cytotoxicity of Lomustine (CCNU) in vitro.



CCNU was used to treat human glioma cell line BT-325 with different concentration of cytokines or NO donors, NO levels was measured by Griess assay and cell survival was evaluated by MTT assay.



(1) Pretreatment with IL-1 beta and LPS markedly suppressed CCNU cytotoxicity in BT-325 cells with a significant increase in NO production (P<0.05). This function could be inhibited by L-NAME. (2) DETA NONOate suppressed cytotoxicity of CCNU to BT  -325 cells in a dose-dependent manner (P<0.05). (3) CCNU co-cultured with SNAP for 24 h showed higher cytotoxic to BT-325 cells(P<0.05).



NO partly suppresses cytotoxicity of Lomustine, which might be associated with chemoresistance of BT-325 cells against CCNU in vitro. NO can also slow the degradation of CCNU in water solution.
Gao
Run-lin
RL
College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Zeng-liang
ZL
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008130:Lomustine / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*
D018906:Antineoplastic Agents, Alkylating; D007375:Interleukin-1; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D008130:Lomustine; D019331:NG-Nitroarginine Methyl Ester