Local RAD50 gene delivery induces regression of preformed porcine coronary in-stent neointimal hyperplasia.
BACKGROUND
Recently, we observed that overexpression of human RAD50 (hRAD50) induced p21-dependent cytotoxicity in various cultured cells, and rat and mouse tumor models. This study investigated the characteristics of endothelial cell (EC) death by hRAD50 and the potential utility of hRAD50 in the development of gene therapies for vascular restenosis.


METHODS
We studied the effects of transient hRAD50 gene transfer using nonliposomal lipid on the survival of primary cultured human coronary arterial EC and smooth muscle cells (SMC). Palmaz-Schatz stents were deployed in two epicardial coronary arteries in each pig (n = 10). Two weeks later, the patency of the stented arteries was documented by coronary angiography, and the hRAD50 construct or empty vector mixed with lipid was delivered to one of the stented arteries in each pig using a Dispatch catheter. Coronary angiography was repeated 2 weeks after gene delivery and histological examination was performed.


RESULTS
Lipid-mediated hRAD50 gene transfer resulted in the death of EC and SMC. It also increased endothelial nitric oxide synthase (eNOS) expression and nitrite production as well as p21 expression. Pretreatment with NOS and pan-caspase inhibitors completely prevented EC death by hRAD50. In the hRAD50-delivered arteries, the percentage of diameter stenosis, neointimal area, and pathologic area of stenosis were significantly smaller than in the control arteries. eNOS expression increased in the hRAD50-delivered arteries. Systemic hematologic and chemical values were not affected by gene delivery.


CONCLUSIONS
Significant regression of preformed in-stent neointimal hyperplasia was induced by local hRAD50 gene delivery to stented porcine coronary arteries without apparent systemic toxicity.
Ahn
Young Keun
YK
Department of Cardiovascular Medicine, Chonnam National University Medical School, Kwangju 501-190, South Korea.
Kook
Hyun
H
Jeong
Myung Ho
MH
Ahn
Kyu Youn
KY
Cho
Jeong Gwan
JG
Park
Jong Chun
JC
Kang
Jung Chaee
JC
Kim
Kyung Keun
KK
D017766:Acid Anhydride Hydrolases; D000818:Animals; D018929:Cell Culture Techniques; D016923:Cell Death; D017023:Coronary Angiography; D023903:Coronary Restenosis / Q000628:therapy*; D045643:DNA Repair Enzymes / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D042783:Endothelial Cells; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006965:Hyperplasia; D008055:Lipids; D009130:Muscle, Smooth / Q000166:cytology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D015607:Stents; D013552:Swine
D004268:DNA-Binding Proteins; D008055:Lipids; D019001:Nitric Oxide Synthase; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D016283:Proto-Oncogene Proteins p21(ras); D045643:DNA Repair Enzymes