Heterologous expression of Zygosaccharomyces rouxii glycerol 3-phosphate dehydrogenase gene (ZrGPD1) and glycerol dehydrogenase gene (ZrGCY1) in Saccharomyces cerevisiae.
We examined the effects of heterologous expression of the open reading frames (ORF) of two genes on salt tolerance and glycerol production in a Saccharomyces cerevisiae strain deficient in glycerol synthesis (gpd1Deltagpd2Delta). When the ORF of the Zygosaccharomyces rouxii glycerol 3-phosphate dehydrogenase gene (ZrGPD1) was expressed under the control of the GAL10 promoter, salt tolerance and glycerol production increased; when the ORF of the glycerol dehydrogenase gene (ZrGCY1) was expressed under the control of the GAL1 promoter, no such changes were observed. Zrgcy1p had a weak effect on glycerol production. These results suggest that Zrgpd1p is the primary enzyme involved in Z. rouxii glycerol production, following a mechanism similar to that of S. cerevisiae (Gpd1p). When the ORFs of the S. cerevisiae glycerol 3-phosphatase gene (GPP2) and ZrGPD1 were simultaneously expressed, glycerol production increased, compared with that in yeast expressing only ZrGPD1.
Watanabe
Yasuo
Y
Department of Biological Resources, Faculty of Agriculture, Ehime Univeristy, Matsuyama, Ehime790-8566, Japan. watanabe@agr.ehime-u.ac.jp
Tsuchimoto
Syoko
S
Tamai
Youichi
Y
D003001:Cloning, Molecular / Q000379:methods; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D007700:Kinetics; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics*; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
D011994:Recombinant Proteins; D012965:Sodium Chloride; D005993:Glycerolphosphate Dehydrogenase; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005990:Glycerol