[Detection of GGT-II by dot-ELISA with monoclonal antibody in the diagnosis of hepatocellular carcinoma].
OBJECTIVE
Hepatoma-specific gamma- glutamyltransferase isoenzyme II(GGT-II) is considered as the best hepatoma marker except alpha-fetoprotein (AFP), but there is no simple and easy method to determine it now. The purpose of this study was to explore the value of detection of GGT-II by dot-ELISA with monoclonal antibody in the diagnosis of hepatocellular carcinoma (HCC).


METHODS
GGT-II was purified and then BALB/c mouse was immunized. The monoclonal antibody against GGT-II was raised by the hybridoma technique. Serum GGT-II was detected in 123 cases with hepatocellular carcinoma and 164 cases with various benign liver diseases using both dot-ELISA and electrophoresis simultaneously.


RESULTS
The positive rate of serum GGT-II in HCC by dot-ELISA was 71.5%, which was not significantly different from that by electrophoresis (76.4%). However, the false positive rates of GGT-II by dot-ELISA in liver cirrhosis (23.7%) and chronic hepatitis (27.1%) were significantly higher than those by electrophoresis (10.0% and 8.4% for liver cirrhosis and chronic hepatitis, respectively).


CONCLUSIONS
Detection of GGT-II by dot-ELISA with monoclonal antibody is helpful for the diagnosis of HCC, but its diagnostic specificity deserves to be improved.
Ni
Run-Zhou
RZ
Department of Gastroenterology, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu, 226001, P.R.China. nirz@public.nt.js.cn
Huang
Jie-Fei
JF
Xiao
Ming-Bing
MB
Zhang
Pei-Yun
PY
Meng
Xian-Yong
XY
D000328:Adult; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D005723:gamma-Glutamyltransferase / Q000097:blood* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D005723:gamma-Glutamyltransferase