Pretreatment effect of adenosine on activation of NF-kappaB and level of TNF-alpha during myocardial ischemia and reperfusion in rats.
OBJECTIVE
To study the pretreatment effect of adenosine on NF-kappaB nuclear activity in ischemia/reperfusion myocardium in rats.


METHODS
Eighteen healthy male S-D rats were randomly divided into three groups. Group I was the control group. The other two groups were subjected to 30 minutes of ischemia, followed by 2 hours of reperfusion. In group III, adenosine was given 40 microg.kg(-1).min(-1) 30 minutes before coronary artery occlusion. The NF-kappaB in nuclear was extracted and measured with western blot analysis. TNF-alpha levels in myocardium were measured with enzyme-linked immunosorbent assay (ELISA) system. All the data were recorded with mean +/- SEM, differences at the 95% confidence level were considered significant.


RESULTS
NF-kappaB activity in the nucleus significantly increased after ischemia/reperfusion and TNF-alpha levels changed. Adenosine significantly inhibited NF-kappaB activity in nucleus, and concomitantly decreased the level of TNF-alpha in myocardium.


CONCLUSIONS
Adenosine modulation of NF-kappaB activation may be the cellular molecular mechanism of decreasing of TNF-alpha. The cardioprotective action of adenosine may be involved in the differential modulation of NF-kappaB activation during myocardial ischemia/reperfusion.
Ke
Jian-juan
JJ
Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China. kjj428@sohu.com
Wang
Yan-lin
YL
Li
Jian-guo
JG
Wu
Jing
J
Feng
Xiao-bo
XB
D000241:Adenosine / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D016001:Confidence Intervals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D017202:Myocardial Ischemia / Q000628:therapy*; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D016328:NF-kappa B / Q000032:analysis* / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000187:drug effects
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000241:Adenosine