[Molecular-genetic polymorphisms of cultivars of common hops (Humulus lupulus L.) using ISSR-PCR analysis].
Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulus L.) was studied using the ISSR-PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for certain cultivars were revealed. Based on the coefficient of dissimilarity values, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR-PCR analysis in selective studies aimed at the classification and identification of common hop cultivars are discussed.
Danilova
T V
TV
Timiryazev Agricultural Academy, Moscow, 127550 Russia.
Danilov
S S
SS
Karlov
G I
GI
D027582:Humulus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic