A transient RNA interference assay system using Arabidopsis protoplasts.
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
An
Chung-Il
CI
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Sawada
Aki
A
Fukusaki
Ei-ichiro
E
Kobayashi
Akio
A
D017360:Arabidopsis / Q000235:genetics*; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D011523:Protoplasts / Q000378:metabolism; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D014162:Transfection
D017931:DNA Primers; D012330:RNA, Double-Stranded