Isolation and characterisation of resistant-to-fermentation carbohydrate polymers from cultures of Rhizopus nigricans grown on agrofood waste materials.
Rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. In all cultures, a carbohydrate polymer fraction remained resistant to fermentation. These fractions were isolated in gram amounts and characterised. The molecular weight distribution of the fractions and its sugar composition resembles those of the hairy-regions of the pectins. In the fractions, four main carbohydrates were found: 4-7 mol% Rha, 42-59 mol% Ara, 7-14 mol% Gal, 17-33 mol% GalA.
Hellín
P
P
Department of Food Science and Technology and Human Nutrition, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Laencina
J
J
Ros
J M
JM
D000383:Agriculture / Q000379:methods; D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D003247:Conservation of Natural Resources; D005285:Fermentation / Q000502:physiology; D019649:Food Industry / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D007220:Industrial Waste / Q000517:prevention & control; D010936:Plant Extracts / Q000378:metabolism*; D011096:Polygalacturonase / Q000378:metabolism; D012233:Rhizopus / Q000145:classification / Q000378:metabolism*; D018505:Waste Management / Q000379:methods*
D002241:Carbohydrates; D007220:Industrial Waste; D010936:Plant Extracts; D011096:Polygalacturonase