title: Guar seed beta-mannan synthase is a member of the cellulose synthase super gene family.
abstract: Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (ManS), that makes the beta-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase-like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in beta-glycan formation in plants.
authors.affiliation: Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, 7300 NW 62nd Avenue, Johnston, IA 50131, USA. Kanwarpal.Dhugga@Pioneer.Com
authors.forename: Kanwarpal S
authors.initials: KS
authors.lastname: Dhugga
authors.forename: Roberto
authors.initials: R
authors.lastname: Barreiro
authors.forename: Brad
authors.initials: B
authors.lastname: Whitten
authors.forename: Kevin
authors.initials: K
authors.lastname: Stecca
authors.forename: Jan
authors.initials: J
authors.lastname: Hazebroek
authors.forename: Gursharn S
authors.initials: GS
authors.lastname: Randhawa
authors.forename: Maureen
authors.initials: M
authors.lastname: Dolan
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Kinney
authors.forename: Dwight
authors.initials: D
authors.lastname: Tomes
authors.forename: Scott
authors.initials: S
authors.lastname: Nichols
authors.forename: Paul
authors.initials: P
authors.lastname: Anderson
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D020134:Catalytic Domain; D002482:Cellulose / Q000096:biosynthesis; D031293:Cyamopsis / Q000201:enzymology* / Q000235:genetics; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D005690:Galactose / Q000031:analogs & derivatives; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D008351:Mannans / Q000096:biosynthesis* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000201:enzymology*; D013025:Glycine max / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D008351:Mannans; C081692:beta-1,4-mannan; C012990:galactomannan; D002482:Cellulose; D005964:Glucosyltransferases; D008364:Mannosyltransferases; C021542:mannan synthetase; D008358:Mannose; D005690:Galactose