title: Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
abstract: Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
authors.affiliation: Genome Institute of Singapore, Singapore, Republic of Singapore.
authors.forename: Lisa F P
authors.initials: LF
authors.lastname: Ng
authors.forename: Michelle
authors.initials: M
authors.lastname: Wong
authors.forename: Susie
authors.initials: S
authors.lastname: Koh
authors.forename: Eng-Eong
authors.initials: EE
authors.lastname: Ooi
authors.forename: King-Fai
authors.initials: KF
authors.lastname: Tang
authors.forename: Hoe-Nam
authors.initials: HN
authors.lastname: Leong
authors.forename: Ai-Ee
authors.initials: AE
authors.lastname: Ling
authors.forename: Lora V
authors.initials: LV
authors.lastname: Agathe
authors.forename: Jenny
authors.initials: J
authors.lastname: Tan
authors.forename: Edison T
authors.initials: ET
authors.lastname: Liu
authors.forename: Ee-Chee
authors.initials: EC
authors.lastname: Ren
authors.forename: Lee-Ching
authors.initials: LC
authors.lastname: Ng
authors.forename: Martin L
authors.initials: ML
authors.lastname: Hibberd
mesh_terms: D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D014766:Viremia / Q000821:virology*
chemical_list: D012367:RNA, Viral