title: Chemical and toxicological evaluation of methanol extract of Cuscuta reflexa Roxb. stem and Corchorus olitorius Linn. seed on hematological parameters and hepatorenal functions in mice.
abstract: Methanol extract of Cuscuta reflexa Roxb. stem (MECR) contain flavonoids (0.2%) and Corchorus olitorius Linn. seed (MECO) was found to contain steroids and cardenolide glycosides. Effects of multiple weekly dose of MECR (25, 50, 75 mg/kg, i.p.) and MECO (15, 20, 25 mg/kg, i.p.) on liver and kidney functions and hematological parameters in mice were studied. No significant alteration of RBC count and hemoglobin content was observed in all dose level of treatment in MECR and MECO treated mice whereas significant increase of clotting time was seen in moderate and high doses in both case. MECR and MECO both caused significant increase in WBC count only in high dose level of treatment. Both the extracts in medium and high dose level increased SGOT, SGPT, NPN and plasma cholesterol significantly. Serum alkaline phosphatase and total bilirubin were also increased by both moderate and high dose level of treatments in MECR and MECO treated mice respectively. Low dose of both the extract did not exhibit any significant change of creatinine and serum protein level. But high dose level of MECR and MECO significantly increased creatinine level. Increase in plasma cholesterol may be due to decrease in cholesterol catabolism owing to liver dysfunction of due to the intake of MECO itself as it was found to be steroid in nature. Elevated level of SGOT, SGPT and serum alkaline phosphatase activity in moderate and high dose level of weekly treated mice may be due to improper liver function following the treatment. Increased urea, non protein nitrogen and creatinine content in blood have been observed with impaired renal function. The slightly higher toxicity in case of MECO treated mice may be due to the presence of cardenolide glycosides in the ME of C. olitorius seed. However, low doses of MECR and MECO (25 and 15 mg/kg, i.p. respectively) did not exhibit any remarkable change on liver and kidney functions and hematological parameters.
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, West Bengal, India.
authors.forename: Upal Kanti
authors.initials: UK
authors.lastname: Mazumder
authors.forename: Malaya
authors.initials: M
authors.lastname: Gupta
authors.forename: Dilipkumar
authors.initials: D
authors.lastname: Pal
authors.forename: Shiladitya
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects; D001774:Blood Chemical Analysis; D032381:Corchorus / Q000737:chemistry* / Q000633:toxicity; D034182:Cuscuta / Q000737:chemistry* / Q000633:toxicity; D007668:Kidney / Q000187:drug effects*; D007677:Kidney Function Tests; D007958:Leukocyte Count; D008099:Liver / Q000187:drug effects*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D012639:Seeds / Q000737:chemistry
chemical_list: D010936:Plant Extracts