The detection of bovine lactoferrin binding protein on Toxoplasma gondii.
Lactoferrin (LF), a member of the transferrin (TF) protein family, is an iron-binding protein that is known to interact with bacteria through a specific receptor. We examined the binding of bovine LF (bLF), bovine TF (bTF), and ovotransferrin (OTF) by Toxoplasma gondii using a fluorescence test and the streptavidin-biotin (SAB) method using biotin-streptavidin, and found that bLF, bTF, and OTF bound to the protein components of T. gondii. Furthermore, we confirmed that bLF, bTF, and OTF bound a 42 kDa soluble protein of T. gondii by far Western blot method. These results demonstrated that bLF binding proteins are present on T. gondii.
Tanaka
Tetsuya
T
Dairy Science Laboratory, Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan.
Abe
Yukie
Y
Kim
Woan-Sub
WS
Xuan
Xuenan
X
Nagasawa
Hideyuki
H
Igarashi
Ikuo
I
Kumura
Haruto
H
Shimazaki
Kei-ichi
K
D000818:Animals; D001426:Bacterial Proteins; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D003207:Conalbumin / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D015800:Protozoan Proteins / Q000032:analysis*; D014122:Toxoplasma / Q000166:cytology*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D015800:Protozoan Proteins; C027768:lactoferrin binding proteins, bacterial; D003207:Conalbumin; D007781:Lactoferrin