title: [Molecular chaperone GRP75 reprove cells from injury caused by glucose deprivation].
abstract: Glucose-regulated proteins 75(grp75) is a member of hsp70 family. The expression of grp75 is upregulated during glucose starvation (such as ischemia). To evaluate grp75 function, CHL cells were cultured with glucose-free media for 20 h (A) and glucose-free media for 12 h + glucose-containing media for 8 h (ischemia reperfusion) (B). A constructed rat grp75 cDNA expression vector (pcDNA/grp75) was transfected into CHL cells and a cell strain that stably overexpressed grp75 was obtained. The transfected cells and untransfected cells(control group) were cultured with A or B. By MTT, LDH leakage measurement and flow cytometry analysis, growth rate of untransfected cells in B is significantly lower than that in glucose-containing media for 20 h (C) (p < 0.05) and A (p < 0.05). Growth rate of transfected cells is apparently higher than that of control group in B (p < 0.01). LDH liberation percentage of untransfected cells in B is obviously higher than that in C(p < 0.01) and it is not different from A(p > 0.05). LDH liberation percentage of transfected cells is apparently lower than that of control group in B(p < 0.01). Apoptosis of transfected cells is obviously lower by flow cytometry analysis. These results provide evidence for the cytoprotective function of grp75 during glucose starving and ischemia reperfusion.
authors.affiliation: College of Life Science, Nanjing Normal University, Nanjing 210097.
authors.forename: Cui Xiang
authors.initials: CX
authors.lastname: Gao
authors.forename: Shuang Quan
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Yin
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D006224:Cricetinae; D019610:Cytoprotection; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D005947:Glucose / Q000172:deficiency*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007511:Ischemia / Q000150:complications; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C018551:glucose-regulated proteins; D007770:L-Lactate Dehydrogenase; D005947:Glucose