Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.
Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.
Hong
Rui
R
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Fischer
Nicholas O
NO
Verma
Ayush
A
Goodman
Catherine M
CM
Emrick
Todd
T
Rotello
Vincent M
VM
D019187:Cadmium Compounds / Q000737:chemistry*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D005227:Fatty Acids / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D011487:Protein Conformation; D018036:Selenium Compounds / Q000737:chemistry*; D013499:Surface Properties
D019187:Cadmium Compounds; D005227:Fatty Acids; D018036:Selenium Compounds; C016173:undecanoic acid; D011092:Polyethylene Glycols; C058667:cadmium selenide; D002918:Chymotrypsin; C117608:alpha-chymotrypsin