Cloning and functional characterization of resistin-like molecule gamma.
Resistin, a recently discovered hormone that may play a crucial role in obesity-associated diabetes, is the founding member of a novel family of cysteine-rich proteins that are secreted by specific cell types. Three other members of this family have been described to date and were termed resistin-like molecules (RELMs). Here we describe the cloning and functional characterization of RELMgamma. The mouse RELMgamma-cDNA encodes a protein of 117 amino acids that contains a signal peptide leading to secretion of the protein. By Northern blotting the RELMgamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELMgamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELMgamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.
Schinke
Thorsten
T
Department of Trauma and Reconstructive Surgery, Hamburg University, 20246 Hamburg, Germany.
Haberland
Michael
M
Jamshidi
Alaleh
A
Nollau
Peter
P
Rueger
Johannes M
JM
Amling
Michael
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D005819:Genetic Markers; D018922:HL-60 Cells; D006729:Hormones, Ectopic / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution; D014162:Transfection
D000596:Amino Acids; D018076:DNA, Complementary; D005819:Genetic Markers; D006729:Hormones, Ectopic; D021382:Protein Sorting Signals; D012333:RNA, Messenger; C482058:resistin-like gamma, mouse; D003545:Cysteine