title: Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice.
abstract: Site-specific recombinase systems (Cre-loxP, Flp-FRT, and phi C31-att) are transforming both forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be induced in vitro or in vivo, these systems have incited a wave of new biology, advancing our understanding of gene function, genetic relationships, development, and disease.
authors.affiliation: Harvard Medical School, Department of Genetics, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Branda
authors.forename: Susan M
authors.initials: SM
authors.lastname: Dymecki
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D016678:Genome; D019426:Integrases / Q000235:genetics; D051379:Mice / Q000235:genetics*; D023421:Models, Animal; D008967:Molecular Biology / Q000379:methods* / Q000639:trends; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D045522:Recombinases / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D045522:Recombinases; D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases