title: In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation.
abstract: The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.
authors.affiliation: Department of Gene Expression and Development, The Roslin Institute, Midlothian, EH 25 9PS, Scotland, United Kingdom.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.forename: Tim
authors.initials: T
authors.lastname: King
authors.forename: John
authors.initials: J
authors.lastname: Dobrinsky
authors.forename: Linda
authors.initials: L
authors.lastname: Harkness
authors.forename: Tricia
authors.initials: T
authors.lastname: Ferrier
authors.forename: Wim
authors.initials: W
authors.lastname: Bosma
authors.forename: Lori L
authors.initials: LL
authors.lastname: Schreier
authors.forename: H David
authors.initials: HD
authors.lastname: Guthrie
authors.forename: Paul
authors.initials: P
authors.lastname: DeSousa
authors.forename: Ian
authors.initials: I
authors.lastname: Wilmut
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D046508:Culture Techniques; D004558:Electric Stimulation; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000502:physiology*; D010060:Ovulation; D010312:Parthenogenesis / Q000502:physiology; D011247:Pregnancy; D013552:Swine / Q000196:embryology*