Changes in Salmonella enterica serovar Oranienburg viability caused by NaCl-induced osmotic stress is related to DNA relaxation by the H-NS protein during host infection.
The NaCl sensitivity of Salmonella enterica serovar Oranienburg strains depends on their origin. We found previously that food- and patient-origin isolates in an outbreak were, respectively, NaCl-resistant and NaCl-sensitive, and the NaCl-resistant strain of food-origin isolates became NaCl-sensitive after passage of the strain through mice [FEMS Microbiol. Lett. 212 (2002) 87]. Here, we report that this phenotypic difference is mainly dependent on topological changes regulated by H-NS, a bacterial histone-like nucleoid protein that binds non-specifically to DNA. That is, this phenotypic difference was caused by changes in DNA topology during infection of the host. Based on these findings, we propose this mechanism has a key role in promoting the survival of Salmonella under osmotic stress.
Asakura
Hiroshi
H
Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080-8555, Hokkaido, Japan
Kawamoto
Keiko
K
Shirahata
Toshikazu
T
Makino
Sou-ichi
S
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000502:physiology*; D049832:Decapodiformes / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D009997:Osmotic Pressure; D010957:Plasmids / Q000378:metabolism; D012478:Salmonella Food Poisoning / Q000382:microbiology; D012480:Salmonella Infections / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D012808:Sigma Factor / Q000096:biosynthesis / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013154:Spleen / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D012965:Sodium Chloride