title: The detection of bovine lactoferrin binding protein on Toxoplasma gondii.
abstract: Lactoferrin (LF), a member of the transferrin (TF) protein family, is an iron-binding protein that is known to interact with bacteria through a specific receptor. We examined the binding of bovine LF (bLF), bovine TF (bTF), and ovotransferrin (OTF) by Toxoplasma gondii using a fluorescence test and the streptavidin-biotin (SAB) method using biotin-streptavidin, and found that bLF, bTF, and OTF bound to the protein components of T. gondii. Furthermore, we confirmed that bLF, bTF, and OTF bound a 42 kDa soluble protein of T. gondii by far Western blot method. These results demonstrated that bLF binding proteins are present on T. gondii.
authors.affiliation: Dairy Science Laboratory, Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yukie
authors.initials: Y
authors.lastname: Abe
authors.forename: Woan-Sub
authors.initials: WS
authors.lastname: Kim
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Haruto
authors.initials: H
authors.lastname: Kumura
authors.forename: Kei-ichi
authors.initials: K
authors.lastname: Shimazaki
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D003207:Conalbumin / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D015800:Protozoan Proteins / Q000032:analysis*; D014122:Toxoplasma / Q000166:cytology*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D015800:Protozoan Proteins; C027768:lactoferrin binding proteins, bacterial; D003207:Conalbumin; D007781:Lactoferrin