[Effect of Ca2+ ions on hydrodynamic properties of pentamer and decamer of C-reactive protein in solution].
The molecular mass and sedimentation coefficient of native C-reactive protein in solution were determined by analytical ultracentrifugation in the presence and absence of calcium ions. Pentameric C-reactive protein was shown to be the major macroscopic form of this protein in solution. The removal of calcium ions from solution caused decompaction of the protein accompanied by changes in its hydrodynamic parameters. The sedimentation coefficient s20(0), w of pentameric C-reactive protein in solution containing 2 mM--Ca2+ (6.6S) exceeded that for C-reactive protein in solution containing 2 mM EDTA (6.4S). Analysis of average molecular masses Mw and Mz obtained from sedimentation data demonstrated that the solution of highly purified protein was not homogeneous. As shown by intermolecular crosslinking, the solution also contained the 241-kDa decamer of C-reactive protein (9.5S) as a separate macroscopic form, whose share hardly reached 10% in the presence of 2 mM Ca2+ and increased after removal of calcium ions. The decamers were shown to result from intermolecular association of the pentamers.
Blizniukov
O P
OP
State Research Center, Institute of Immunology, Russian Ministry of Health, Moscow, 115478 Russia. blisnukov@mail.ru
Kozmin
L D
LD
Falikova
V V
VV
Martynov
A I
AI
Tishchenko
V M
VM
D002097:C-Reactive Protein / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D007477:Ions; D008970:Molecular Weight; D012996:Solutions
D007477:Ions; D012996:Solutions; D002097:C-Reactive Protein; D004492:Edetic Acid; D002118:Calcium