Establishment of transgenic mice carrying gene encoding human zinc finger protein 191.
OBJECTIVE
Human zinc finger protein 191 (ZNF191) was cloned and characterized as a Kruppel-like transcription factor, which might be relevant to many diseases such as liver cancer, neuropsychiatric and cardiovascular diseases. Although progress has been made recently, the biological function of ZNF191 remains largely unidentified. The aim of this study was to establish a ZNF 191 transgenic mouse model, which would promote the functional study of ZNF191.


METHODS
Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. The offsprings were identified by PCR and Southern blot analysis. ZNF 191 gene expression was analyzed by RT-PCR. Transgenic founder mice were used to establish transgenic mouse lineages. The first generation (F1) and the second generation (F2) mice were identified by PCR analysis. Ten-week transgenic mice were used for pathological examination.


RESULTS
Four mice were identified as carrying copies of ZNF191 gene. The results of RT-PCR showed that ZNF 191 gene was expressed in the liver, testis and brain in one of the transgenic mouse lineages. Genetic analysis of transgenic mice demonstrated that ZNF 191 gene was integrated into the chromosome at a single site and could be transmitted stably. Pathological analysis showed that the expression of ZNF 191 did not cause obvious pathological changes in multiple tissues of transgenic mice.


CONCLUSIONS
ZNF 191 transgenic mouse model would facilitate the investigation of biological functions of ZNF191 in vivo.
Li
Jian-Zhong
JZ
Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
Chen
Xia
X
Yang
Hua
H
Wang
Shui-Liang
SL
Gong
Xue-Lian
XL
Feng
Hao
H
Guo
Bao-Yu
BY
Yu
Long
L
Wang
Zhu-Gang
ZG
Fu
Ji-Liang
JL
D000818:Animals; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D011247:Pregnancy; D014781:Viscera / Q000473:pathology / Q000502:physiology
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; C457300:ZNF24 protein, human