[Electron microscopic observation of primary cultured laterally spreading tumor cell line].
OBJECTIVE
To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary culture.


METHODS
The cells isolated from a rectum LST specimen obtained by endoscopic mucosal resection was primary cultured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocarcinoma and normal mucosa of the rectum.


RESULTS
Scanning and transmission electron microscopes both revealed numerous microvilli covering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes. Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli.


CONCLUSIONS
The cultured LST cells are highly malignant.
Han
Yu-jing
YJ
Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Lai
Zhuo-sheng
ZS
Wang
Ya-dong
YD
Jiang
Bo
B
Zhou
Zhi-tao
ZT
Zhou
Dian-yuan
DY
D000230:Adenocarcinoma / Q000648:ultrastructure*; D045744:Cell Line, Tumor; D006801:Humans; D008854:Microscopy, Electron; D012004:Rectal Neoplasms / Q000648:ultrastructure*