title: Automated procedure for improving the RNA isolation step in viral load testing for human immunodeficiency virus.
abstract: An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation procedure.
authors.affiliation: Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Yung
authors.initials: Y
authors.lastname: Chan
authors.forename: Steven H
authors.initials: SH
authors.lastname: Fischer
authors.forename: Alan T
authors.initials: AT
authors.lastname: Remaley
mesh_terms: D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification*; D019562:Viral Load
chemical_list: D012367:RNA, Viral