title: PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A.
abstract: Spontaneous polymyxin-resistant mutants of Pseudomonas aeruginosa were isolated. The mutations responsible for this phenotype were mapped to a two-component signal transduction system similar to PmrAB of Salmonella enterica serovar Typhimurium. Lipid A of these mutants contained aminoarabinose, an inducible modification that is associated with polymyxin resistance. Thus, P. aeruginosa possesses a mechanism that induces resistance to cationic antimicrobial peptides in response to environmental conditions.
authors.affiliation: Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington 98195, USA.
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Moskowitz
authors.forename: Robert K
authors.initials: RK
authors.lastname: Ernst
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
mesh_terms: D023181:Antimicrobial Cationic Peptides; D001089:Arabinose / Q000031:analogs & derivatives* / Q000737:chemistry*; D001426:Bacterial Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008050:Lipid A / Q000737:chemistry*; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000494:pharmacology*; D011112:Polymyxin B / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D023083:beta-Defensins / Q000494:pharmacology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; C081140:C18G peptide; C106641:DEFB4A protein, human; D008050:Lipid A; D010455:Peptides; C409039:PmrB protein, bacteria; D011506:Proteins; D014157:Transcription Factors; C016982:aminoarabinose; D023083:beta-Defensins; C094880:protegrin-1; D001089:Arabinose; D011112:Polymyxin B