Inhibition of B(a)P induced strand breaks in presence of curcumin.
Incidence of cancer at different sites may be related to oxidative damage to host genome by genotoxicants. These oxidative actions may be modified by phytochemicals present in foods. The non-nutritive dietary constituents which possess antimutagenic property appear to be promising chemopreventive agents. This study reports the protective effect of curcumin on B(a)P induced DNA damage in human peripheral blood lymphocyte cells. The study group consisted of 10 male smokers, 10 non-smokers and 10 non-smoking females aged between 25 and 45. The DNA damage was assessed using comet assay. In all the groups curcumin showed a dose-dependent inhibitory effect. The effect appeared to be sex dependent. There was no correlation between DNA damage and GST-Mu levels and levels of micronutrients namely Vitamins A, E and beta carotene. The results of this study are in line with our earlier observations on turmeric/curcumin as a potential chemopreventer.
Polasa
K
K
National Institute of Nutrition, Indian Council of Medical Research, Hyderabad 500007, India. kpolasa@yahoo.com
Naidu
A N
AN
Ravindranath
I
I
Krishnaswamy
K
K
D000328:Adult; D000975:Antioxidants / Q000032:analysis; D001564:Benzo(a)pyrene / Q000633:toxicity*; D020552:Comet Assay; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged
D000975:Antioxidants; D001564:Benzo(a)pyrene; D005982:Glutathione Transferase; D003474:Curcumin