title: A first generation bovine BAC-based physical map.
abstract: A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105,984 clones) and a part of the CHORI-240 BAC library (26,500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100,923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.
authors.affiliation: Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas, France. schibler@jouy.inra.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Schibler
authors.forename: Anne
authors.initials: A
authors.lastname: Roig
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Mahé
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Save
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gautier
authors.forename: Sead
authors.initials: S
authors.lastname: Taourit
authors.forename: Didier
authors.initials: D
authors.lastname: Boichard
authors.forename: André
authors.initials: A
authors.lastname: Eggen
authors.forename: Edmond P
authors.initials: EP
authors.lastname: Cribiu
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D016172:DNA Fingerprinting; D005819:Genetic Markers; D016678:Genome; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers