Glutathione S-transferase P1 has protective effects on cell viability against camptothecin.
Glutathione S-transferase P1 (GSTP1) is one of the important xenobiotic-metabolizing enzymes. It was reported that GSTP1 was overexpressed in malignant tissues, and its expression level was associated with resistance to chemotherapeutics. We carried out transfection of GSTP1 sense and antisense vectors to examine effects of GSTP1 on cell cycle arrest and apoptosis induced by camptothecin in HeLa cells. Transfection of GSTP1 antisense vector induced apoptosis. Camptothecin-induced S- or G2/M arrest was intensified by transfection of GSTP1 antisense vector, and subsequent apoptosis was attenuated by transfection of GSTP1 sense vector. These results suggest that GSTP1 has protective effects against camptothecin-induced cytotoxicity.
Hara
Takamitsu
T
Department of Radiology, Yokohama City University School of Medicine, Yokohama City, Japan.
Ishii
Takeo
T
Fujishiro
Mina
M
Masuda
Michiaki
M
Ito
Takaaki
T
Nakajima
Jun
J
Inoue
Tomio
T
Matsuse
Takeshi
T
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis; D002166:Camptothecin / Q000494:pharmacology*; D002453:Cell Cycle; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016195:G2 Phase; D005822:Genetic Vectors; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008280:Magnetics; D008938:Mitosis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016196:S Phase; D013997:Time Factors; D014162:Transfection; D015262:Xenobiotics / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D016376:Oligonucleotides, Antisense; D015262:Xenobiotics; D004247:DNA; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D002166:Camptothecin