Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material.
The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region.
Nicholls
John
J
Department of Pathology, The University of Hong Kong, Hong Kong.
Hahn
Peter
P
Kremmer
Elisabeth
E
Fröhlich
Thomas
T
Arnold
Georg J
GJ
Sham
Jonathan
J
Kwong
Dora
D
Grässer
Friedrich A
FA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D001706:Biopsy; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020031:Epstein-Barr Virus Infections / Q000821:virology; D005814:Genes, Viral; D006689:Hodgkin Disease / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000821:virology*; D016411:Lymphoma, T-Cell, Peripheral / Q000821:virology*; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009856:Oncogene Proteins, Viral; D014763:Viral Matrix Proteins