ABRF-ESRG'03: analysis of a PVDF-bound known protein with a homogeneous amino-terminus.
The Association of Biomolecular Resource Facilities 2003 Edman Sequencing Research Group (ABRF-ESRG'03) sample is the 15th in a series of studies designed to allow participating members to evaluate their abilities to analyze the N-terminus of a protein or peptide using automated Edman degradation chemistry. It is a follow-up study to the ESRG'02 sample, which was a single protein with a heterogeneous N-terminus. Both the 2002 and 2003 samples were obtained from the same protein complex and were resolved by SDS-PAGE followed by electrophoretic transfer to PVDF membrane. The ABRF-ESRG'03 sample had an apparent molecular weight of 49 kDa and a single N-terminus, with initial yields of approximately 2 pmol. Participants were asked to sequence 25 residues and return their results to the ESRG for analysis along with two completed surveys and an area/pmol table for repetitive and initial yield calculations. Data for 46 responses are presented which include initial yields, repetitive yields, sequencer performance, and ability to identify the protein.
Buckel
Scott D
SD
Amgen, Boulder, CO, USA.
Cook
Richard G
RG
Crawford
J Myron
JM
Denslow
Nancy
N
Fernandez
Joseph
J
Madden
Ben J
BJ
Neveu
John M
JM
D000595:Amino Acid Sequence; D003625:Data Collection; D016208:Databases, Factual; D004591:Electrophoresis, Polyacrylamide Gel; D011145:Polyvinyls; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D005465:Fluorocarbon Polymers
D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers