The sharing of target-epitopes between human anti-ABO hemagglutinating and anti-Pig (xeno) endothelium or anti-Pig thyroglobulin antibodies.
To select congenial pairs between donor-pig and recipient-human for the future xenotransplantation, the levels of xeno-IgM natural antibodies (NAb) were analyzed in healthy subjects and hemodialysis patients by ELISA tests, which target swine-derived crude endothelial cells (P16N) or proteins (thyroglobulin; TG). The total IgM concentration was lower in hemodialysis patients than in healthy subjects, but there was no difference in IgM NAb titer between the two groups. Individuals with non-B blood types (A, O) exibited significantly higher IgM NAb titer compared with those with B blood types (B, AB). A blood type individuals showed higher killing activity against P16N than those with B, AB or O types with a statistical significance. Sera from A blood type, after being absorbed with red blood cells (RBC) from B blood type, decreased their IgM titer against TG to the level of sera from B blood type. Meanwhile, sera from A blood type significantly decreased hemagglutinin titer against B-RBC after passage through a TG-coated affinity column. We conclude that human anti-B-RBC and anti-Pig xeno NAb have certain common binding epitopes, which might be a branched B carbohydrate structure.
Morita
Naokatsu
N
Department of Nephrology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Hagihara
Masao
M
Kanai
Nobuyuki
N
Hiraga
Seigo
S
Saito
Akira
A
Sakai
Hideto
H
Tsuji
Kimiyoshi
K
D000017:ABO Blood-Group System / Q000276:immunology*; D000042:Absorption; D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood*; D000920:Antibody-Dependent Cell Cytotoxicity; D016022:Case-Control Studies; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006384:Hemagglutination / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007518:Isoantibodies / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D006435:Renal Dialysis; D013552:Swine; D013954:Thyroglobulin / Q000276:immunology*
D000017:ABO Blood-Group System; D000910:Antibodies, Heterophile; D000939:Epitopes; D007075:Immunoglobulin M; D007518:Isoantibodies; D013954:Thyroglobulin