title: Transplantation of cultured human corneal endothelial cells.
abstract: OBJECTIVE
  To investigate the feasibility of corneal reconstruction utilizing cultured human corneal endothelial cells (HCECs).
  
  
  METHODS
  We cultivated HCECs using culture dishes pre-coated with bovine corneal endothelial extracellular matrix. The effect of donor age on HCECs was investigated. We reconstructed corneas using cultured HCECs and human corneal stroma, then examined their functioning. The possibility of porcine corneal stroma as a carrier of cultured HCECs was investigated.
  
  
  RESULTS
  The older the donor, the more frequently large senescent cells appeared in the passaged HCECs. The density of HCECs on the reconstructed cornea reached 2500 cells/mm2. The potential difference in the reconstructed and normal corneas was 0.30 mV and 0.40 mV, respectively; this indicates that the pump function of the reconstructed corneas is 75% of that of normal corneas. Porcine corneal stroma expressing little xenosugar antigen alpha-gal epitope induced no superacute rejection but mild cellular rejection when transplanted into corneas of animals possessing natural antibody to alpha-gal epitope.
  
  
  CONCLUSIONS
  To reconstruct corneas that are the same as, or superior to, normal corneas, innovation is necessary in the methods used for culturing and seeding HCECs. Porcine corneal stroma is promising as a carrier of HCECs instead of human corneal stroma, the supply of which is limited. The validity of porcine corneal stroma, acellularized to prevent retrovirus infection, should be evaluated.
authors.affiliation: Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan. amanos-tky@umin.ac.jp
authors.forename: Shiro
authors.initials: S
authors.lastname: Amano
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000032:analysis; D002417:Cattle; D002452:Cell Count; D017690:Cell Transplantation; D002478:Cells, Cultured; D002675:Child, Preschool; D003315:Cornea / Q000473:pathology / Q000601:surgery*; D003319:Corneal Stroma / Q000276:immunology / Q000637:transplantation; D004728:Endothelium, Corneal / Q000166:cytology*; D006084:Graft Rejection; D006801:Humans; D007223:Infant; D019651:Plastic Surgery Procedures; D013552:Swine; D014312:Trisaccharides / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D014312:Trisaccharides; C076569:alpha-galactosyl epitope