title: Genistein replacement therapy for vasodilation disorder in bilateral ovariectomized rats.
abstract: The objective of this study is to examine the effects of genistein on endothelial dysfunction in bilateral ovariectomized rats. Female Wistar rats were subjected to a bilateral ovariectomy (OVX rat). The animals were divided into three groups: sham treated with vehicle (DMSO 100 microliters/day, Shamveh), OVX treated with vehicle (DMSO 100 microliters/day, OVXveh), and OVX treated with genistein (0.25 mg/kg BW/day, OVXgen). Mean arterial pressure (MAP), heart rate (HR), body weight (BW), uterine weight and plasma E2 were monitored at 4-week after the treatment. We investigated the endothelium-dependent and -independent vasorelaxation by using acetylcholine (Ach 10(-6) M) and sodium nitroprusside (SNP 10(-7) M), respectively. The experimental results indicated that the uterine weights of all OVX rats were significantly decreased as compared to the sham groups (OVXveh = 0.007+/-0.004 g, OVXgen =0.003+/-0.001 g, Shamveh =0.017+/-0.001 g). MAP of OVXveh group was significantly increased compared to the Sham group (OVXveh=139.99+/-7.50 mmHg, Shamveh =118.10+/-19.33 mmHg, p<0.05). No significant increase in MAP was observed in OVXgen (OVXgen =123.33+/-8.61 mmHg; p<0.05). HR showed no significant difference among those groups. The present study of vasodilator responses demonstrated only the significant decrease in endothelium-dependent, not for endothelium-independence, in OVX rats, while the treatment of genistein could significantly attenuate this abnormality (OVXveh =3.03+/-3.99%, Shamveh =45.46+/-3.59%, OVXgen =33.52+/-3.25% in % change of vessel diameter). The present findings suggest that genistein could be used as a therapeutic agent for menopausal vascular complications.
authors.affiliation: Interdepartment of Physiology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Khemapech
authors.forename: K
authors.initials: K
authors.lastname: Monsiri
authors.forename: S
authors.initials: S
authors.lastname: Patumraj
authors.forename: P
authors.initials: P
authors.lastname: Siriviriyakul
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology; D004958:Estradiol / Q000097:blood; D005260:Female; D019833:Genistein / Q000627:therapeutic use*; D006339:Heart Rate / Q000187:drug effects; D007082:Ileum / Q000098:blood supply; D007529:Isoflavones / Q000627:therapeutic use*; D009599:Nitroprusside / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D048789:Phytoestrogens; D028321:Plant Preparations / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000187:drug effects; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; D009599:Nitroprusside; D004958:Estradiol; D019833:Genistein; D000109:Acetylcholine; D009638:Norepinephrine