In vitro transfection of the human vas deferens using DNA-liposome and DNA-neutral lipid complexes.
OBJECTIVE
To transfect the human vas deferens in vitro.


METHODS
Prospective study, description of a procedure.


METHODS
Research center and university hospital.


METHODS
Seven fertile men undergoing vasectomies or vasovasostomies.


METHODS
Human vas deferens pieces were transfected in vitro using the p-GeneGrip gene construction, which codifies for the green fluorescent protein (GFP). Lipofectamine or GenePorter were employed as gene vectors.


METHODS
After vas deferens epithelium transfection, we described the vas deferens foreign gene expression. Maximum transfection occurred in 14.7% of the vas deferens epithelial cells. After using GenePorter, we observed green fluorescent protein gene expression in 40% of samples, which occupied 9.86% of the epithelial area. After Lipofectamine treatment, transgene expression occurred in 33% of the samples and occupied 9.05% of the epithelial area.


CONCLUSIONS
The human vas deferens epithelium has the potential to be modified by gene transfection.
Esponda
Pedro
P
Centro de Investigaciones Biológicas, CSIC, Madrid, Spain. esponda@cib.csic.es
Goldstein
Marc
M
Witkin
Steve S
SS
D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D011446:Prospective Studies; D014162:Transfection / Q000379:methods*; D014649:Vas Deferens / Q000166:cytology / Q000502:physiology*
D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D004247:DNA