title: [Stability studies of sulfated polyguluronic acid ester].
abstract: OBJECTIVE
  To test the stability of marine polysaccharide drug sulfated polyguluronic acid ester.
  
  
  METHODS
  Four methods including high performance gel chromatography (HPGC), poly-acrylamide gel electrophoresis (PAGE), UV scan of absorbance between 200 and 800 nm and gelatin nephelometry were established. Samples were tested in high temperature, high humidity, strong light and accelerated test conditions. The methods were used to test the changes of the parameters including molecular weight, molecular weight distribution, absorbance between 200 and 800 nm, free sulfate, with which we could estimate the stability of sulfated polyguluronic acid ester could be estimated.
  
  
  RESULTS
  The four methods were suitable to test the stability of sulfated polyguluronic acid ester and the sample were stable in the conditions as before except in high temperature.
  
  
  CONCLUSIONS
  Sulfated polyguluronic acid ester has good stability.
authors.affiliation: Marine Drug and Food Institute, Ocean University of Qing Dao, Qingdao 266003, China. zzq0127@hotmail.com
authors.forename: Zhen-qing
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Hua-shi
authors.initials: HS
authors.lastname: Guan
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature
chemical_list: D011135:Polysaccharides, Bacterial; C111091:polyguluronic acid