title: Coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans.
abstract: The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated. By investigation of the activities of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in the soluble fraction of G. oxydans, and cloning and expression of genes in Escherichia coli, it was found that both G6PDH and 6PGDH have NAD/NADP dual coenzyme specificities. It was suggested that the pentose phosphate pathway is responsible for NADH regeneration in G. oxydans.
authors.affiliation: AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki-shi, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Tonouchi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
mesh_terms: D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D020600:Gluconobacter oxydans / Q000201:enzymology* / Q000235:genetics; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003067:Coenzymes; D009243:NAD; D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase