title: [Study of ex vivo expansion of mouse hematopoietic stem/progenitor cells and the use of them for hematopoiesis reconstitution].
abstract: OBJECTIVE
  To explore the rapid neutrophil engraftment and long-term hematopoietic reconstitution.
  
  
  METHODS
  Mononuclear cells (MNCs) were isolated from 5-Fu treated male BDF1 mouse bone marrow and CD(34)(+)/c-kit(+) cells were selected from the MNCs by using MoFlo Cell Sorter. MNCs and CD(34)(+)/c-kit(+) cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) in a two-step expansion. The expanded cells were then transplanted into sublethally irradiated female BDF1 mice.
  
  
  RESULTS
  Co-culture with MSCs resulted in 10.8-, 4.8-, 65.9- and 38.8-fold increases yields of median total nucleated cells, CD(34)(+) cells, GM-CFC and HPP-CFC, respectively, as for the MNCs culture, and 76.1-, 2.9-, 71.7- and 51.8-fold increase respectively for the CD(34)(+)/c-kit(+) cell culture. The expanded cells could rapidly engraft in the sublethally, irradiated mice, reconstitute their hematopoiesis, and be detected in the recipients bone marrow 2 months after transplantation.
  
  
  CONCLUSIONS
  Hematopoietic stem/progenitor cells co-cultures with MSCs in two-step expansion could increase expansion yields of total nucleated cells, GM-CFC and HPP-CFC. The availability of increased numbers of expanded cells may result in more rapid engraftment of neutrophils following infusion to transplant recipients.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310012, China.
authors.forename: Jin-fu
authors.initials: JF
authors.lastname: Wang
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Qiu
authors.forename: Jenny
authors.initials: J
authors.lastname: Harrintong
authors.forename: Ian K
authors.initials: IK
authors.lastname: McNiece
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008297:Male; D051379:Mice
chemical_list: D018952:Antigens, CD34