Immunostaining with dissociable antibody microarrays.
The availability of a large number of biological materials such as cDNA, antibodies, recombinant proteins, and tissues has promoted the development of microarray technologies that make use of these materials in high-throughput screening assays. However, because microarray technologies have been less successful in examining proteins than DNA and mRNA, there is a need for improved protein microarray systems. To address this need, we developed an antibody microarray-based immunostaining method that can analyze the properties of a large number of proteins simultaneously. In this method, antibodies are arrayed and immobilized on a solid support and cells bearing antigens of interest are attached to a second support. Apposition of the two supports allows the antibodies to dissociate from the array support and bind to the cellular antigens. After separation of the supports, antigen-bound antibodies can be detected by standard secondary antibody techniques. These "dissociable" antibody arrays were used to detect both the expression and subcellular localization of a large number of specific proteins in various cultured cell types.
Wang
Yingjian
Y
Hypromatrix Inc, Worcester, MA 01604, USA. ywang@hypomatrix.com
D000906:Antibodies; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D040081:Protein Array Analysis; D013194:Staining and Labeling
D000906:Antibodies