title: Size and structure characterization of ethylhydroxyethyl cellulose by the combination of field-flow fractionation with other techniques. Investigation of ultralarge components.
abstract: Ethylhydroxyethyl cellulose (EHEC) of three different viscosity classes (EHEC I, II, and III) was analyzed by programmed cross-flow asymmetrical flow field-flow fractionation coupled to multiangle light scattering and refractive index detectors to determine their size and molar mass distribution. Two size populations were detected in the two lower viscosity classes, EHEC I and II, one high molar mass and one ultrahigh molar mass (UHM). The two covered molar masses from 10(4) up to 10(9) g X mol(-1). The highest viscosity class EHEC III was less size-dispersed covering molar masses from 5 x 10(5) to 5 x 10(7) g.mol(-1). Filtering of the EHEC II solution removed small amounts of compact UHM material. Enzyme treatments were performed on EHEC II to further characterize it. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and anion ion-exchange chromatography coupled to pulsed amperometric detection showed that the UHM component contained EHEC.
authors.affiliation: Department of Technical Analytical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Andersson
authors.forename: Bengt
authors.initials: B
authors.lastname: Wittgren
authors.forename: Herje
authors.initials: H
authors.lastname: Schagerlöf
authors.forename: Dane
authors.initials: D
authors.lastname: Momcilovic
authors.forename: Karl-Gustav
authors.initials: KG
authors.lastname: Wahlund
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D008968:Molecular Conformation; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014783:Viscosity
chemical_list: C462399:ethyl(hydroxyethyl)cellulose; D002482:Cellulose