title: Changes in properties of glutamate transport in Trichoderma viride vegetative mycelia upon adaptation to glutamate as carbon source.
abstract: The U-(14)C-labelled glutamate uptake was measured in both sucrose- and glutamate-grown mycelia of Trichoderma viride. The biomass yield was five-fold lower with glutamate as a sole carbon source. The rate of glutamate transport measured at a glutamate concentration of 1 mM remained unchanged in glutamate-grown mycelia whereas the properties of the glutamate transport were substantially changed compared to sucrose-grown mycelia. The glutamate uptake in both sucrose- and glutamate-grown mycelia was inhibited by an uncoupler (3,3',4',5-tetrachlorosalicylanilide) but the inhibitory efficiency was higher in the latter. The affinity of the permease to glutamate increased approximately five-fold in the glutamate-grown mycelia (about 76 microM compared to about 16 microM). The pH optimum for glutamate uptake was 4 in sucrose-grown mycelia but the glutamate-grown mycelia had two pH optima, one at pH 4 and the second between pH 6 and 7. The inhibition of glutamate uptake by other amino acids yielded different inhibitory patterns in the two mycelia under study. The glutamate uptake in mycelia of different ages also showed differences in both transport rate and temporal pattern. The results show that the growth of mycelia on glutamate led to the appearance of an additional permease with different properties and suggest that only this permease is operating in mycelia grown on glutamate.
authors.affiliation: Department of Biochemistry and Microbiology, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Richard
authors.initials: R
authors.lastname: Pokorný
authors.forename: Daniela
authors.initials: D
authors.lastname: Hudecová
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Burgstaller
authors.forename: L'udovít
authors.initials: L
authors.lastname: Varecka
mesh_terms: D000222:Adaptation, Physiological; D001692:Biological Transport; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D018698:Glutamic Acid / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D014242:Trichoderma / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D018698:Glutamic Acid; D013395:Sucrose; D002244:Carbon