title: Calcium signaling through phospholipase C activates dendritic cells to mature and is necessary for the activation and maturation of dendritic cells induced by diverse agonists.
abstract: Calcium is an important second messenger in the phospholipase C (PLC) signal transduction pathway. Calcium signaling is involved in many biological processes, including muscle contraction, cellular activation, and cellular proliferation. Dendritic cell (DC) maturation is induced by many different stimuli, including bacterial lipopolysaccharide (LPS), bacterial toxins, inflammatory cytokines, prostaglandins, as well as calcium mobilization. In the present study, we determined the role of the PLC signal transduction pathway in the activation and maturation of human monocyte-derived DCs (MDDCs) induced by diverse agonists. We found that signaling through PLC activates MDDCs to mature and is necessary for LPS, cholera toxin, dibutyryl-cyclic AMP, prostaglandin E2, and the calcium ionophore A23187 to induce MDDC maturation. The results of the present study along with the results of other studies indicate that multiple signaling pathways are involved in the activation of DCs and that inhibition of any of these pathways inhibits the maturation of DCs.
authors.affiliation: Division of Vaccine Research, Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 21201, USA.
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Bagley
authors.forename: Sayed F
authors.initials: SF
authors.lastname: Abdelwahab
authors.forename: Robert G
authors.initials: RG
authors.lastname: Tuskan
authors.forename: George K
authors.initials: GK
authors.lastname: Lewis
mesh_terms: D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D020013:Calcium Signaling; D002454:Cell Differentiation / Q000187:drug effects; D002772:Cholera Toxin / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D000001:Calcimycin; D003994:Bucladesine; D019284:Thapsigargin; D002772:Cholera Toxin; D010738:Type C Phospholipases; D015232:Dinoprostone