Polyelectrolyte-stabilized glucose biosensor based on woodceramics as electrode.
OBJECTIVE
Different biosensors for early diagnosis of diabetes have been discussed increasingly. We demonstrate the applicability of a glucose biosensor for rapid and quantitative determination of glucose in experimental sample solutions.


METHODS
Polyethylenimine (PEI)-stabilized glucose oxidase (GOD) was used for the construction of stable glucose biosensor utilizing a simple manufacture procedure. The stabilized glucose oxidase was immobilized by physical adsorption into activated porous and conductive carbonaceous material-woodceramics that served as the immobilization matrix and the electrochemical transducer. The optimum parameters for the construction of the biosensor and the influences of pH and temperature on the biosensor response were explored.


RESULTS
Optimum results were obtained using soaking solutions of 2300 U/ml GOD, 10 mg/ml mediator toluidine blue O (TBO), and 2.0%, w/v PEI. The sensitivity of the resulting biosensor for glucose was 1.22 microA/mM, with a linear response in the concentration range of 0.5-7 mM. The sensor-to-sensor reproducibility was good, the relative standard deviation (R.S.D.) being less than 8.0%. The storage and operational stabilities of the glucose biosensor were good.


CONCLUSIONS
The glucose biosensor provides reproducible and quantitative information on glucose within minutes and is applicable for the detection of glucose in experimental samples.
Qian
Jun-Min
JM
State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ailisuo@hotmail.com
Suo
Ai-Li
AL
Yao
Yu
Y
Jin
Zhi-Hao
ZH
D001234:Aspergillus niger / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D007700:Kinetics; D012680:Sensitivity and Specificity; D013696:Temperature
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose