[Stability studies of sulfated polyguluronic acid ester].
OBJECTIVE
To test the stability of marine polysaccharide drug sulfated polyguluronic acid ester.


METHODS
Four methods including high performance gel chromatography (HPGC), poly-acrylamide gel electrophoresis (PAGE), UV scan of absorbance between 200 and 800 nm and gelatin nephelometry were established. Samples were tested in high temperature, high humidity, strong light and accelerated test conditions. The methods were used to test the changes of the parameters including molecular weight, molecular weight distribution, absorbance between 200 and 800 nm, free sulfate, with which we could estimate the stability of sulfated polyguluronic acid ester could be estimated.


RESULTS
The four methods were suitable to test the stability of sulfated polyguluronic acid ester and the sample were stable in the conditions as before except in high temperature.


CONCLUSIONS
Sulfated polyguluronic acid ester has good stability.
Zhang
Zhen-qing
ZQ
Marine Drug and Food Institute, Ocean University of Qing Dao, Qingdao 266003, China. zzq0127@hotmail.com
Zhao
Xia
X
Guan
Hua-shi
HS
D002850:Chromatography, Gel / Q000379:methods; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature
D011135:Polysaccharides, Bacterial; C111091:polyguluronic acid