Effect of animal bedding on rat liver endosome acidification.
Animal beddings, such as pine products, and environmental factors are known to induce liver drug-metabolizing cytochrome P450 enzymes. We observed that a change to pine-based rat bedding altered baseline and cAMP-stimulated rates of acidification in rat liver endosomes, apparently by decreasing ATP-dependent proton transport in the presence and absence of chloride. Although cAMP altered phosphorylation of protein kinase B and extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and p38 mitogen-activated protein kinases, changes in housing conditions did not affect baseline or cAMP-stimulated values of these or other selected signaling molecules. We conclude that compounds in rat bedding may alter not only drug metabolism, but also aspects of endocytosis.
Buddaraju
Ajay Kumar V
AK
Medical Service, 11R Research Service, Ann Arbor VA Hospital, 2215 Fuller Road, Ann Arbor, Michigan 48105, USA.
Van Dyke
Rebecca W
RW
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D001513:Beds; D002772:Cholera Toxin / Q000008:administration & dosage / Q000633:toxicity; D000242:Cyclic AMP / Q000378:metabolism; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006799:Housing, Animal; D006863:Hydrogen-Ion Concentration; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011920:Rats, Inbred WF; D015398:Signal Transduction; D013047:Specific Pathogen-Free Organisms; D014934:Wood
D000255:Adenosine Triphosphate; D002772:Cholera Toxin; D000242:Cyclic AMP