Kinetic studies of the interaction between antitumor antibiotics and DNA using quartz crystal microbalance.
OBJECTIVE
Kinetic studies of the interaction process between antitumor antibiotics, Mitomycin C (MMC) and Bleomycin (BLM), and DNA were performed with a novel analytical method, piezoelectric quartz crystal (PQC) impedance analysis.


METHODS
DNA was directly immobilized on the Au-electrode surface of a piezoelectric quartz crystal by adsorption. The DNA-modified piezoelectric sensor was in contact with Mitomycin C and Bleomycin solution, respectively.


RESULTS
The experimental results demonstrated that antitumor antibiotics concentration had an effect on the interaction. A pseudo-first-order kinetic model for the interaction between antitumor antibiotics and DNA was derived to describe the process. All fitted results were well in agreement with the corresponding experimental results. The kinetic parameters, the interaction rate constants (k(MMC) and k(BLM)), were determined by fitting experimental data to the model. At 37 +/- 0.5 degrees C, the k(MMC) and k(BLM) values obtained were 4.56 (+/-0.02) x 10(-3) and 9.11 (+/-0.02) mM(-1) s(-1), respectively.


CONCLUSIONS
Piezoelectric quartz crystal impedance (PQCI) analysis is a very powerful method to study the kinetic process of antitumor drugs and DNA interaction.
Tian
Lu
L
State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Wei
Wanzhi
W
Mao
Youan
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D017097:Electric Impedance; D007700:Kinetics; D008956:Models, Chemical
D000970:Antineoplastic Agents; D004247:DNA