Transplantation of highly purified peripheral-blood CD34+ progenitor cells from related and unrelated donors in children with nonmalignant diseases.
Transplantation of allogeneic stem cells is currently the only curative treatment for some nonmalignant pediatric diseases. We investigated whether transplantation of purified CD34(+) stem cells prevents acute and chronic GvHD and reduces transplant-related mortality. A total of 25 pediatric patients with nonmalignant diseases underwent allogeneic transplantation from 26 donors (matched related n=4, matched or partially matched unrelated n=14, mismatched related n=8). All grafts were purified peripheral-blood CD34(+) stem cells mobilized with G-CSF. Patients received a median of 12.9 x 10(6) CD34(+) progenitor cells with a median of 6.1 x 10(3) contaminating T-lymphocytes per kilogram of body weight. No post transplant immunosuppressive drugs were given for prophylaxis of GvHD. Engraftment was seen in 21 patients. Three patients engrafted after a second transplant and one patient failed to engraft. Two patients had autologous reconstitution 1.5 years post transplant and one of them was successfully retransplanted. No acute GvHD >grade II was seen, and only two patients developed limited, chronic GvHD. In all, 22 patients (88%) are alive with a median follow-up of 3.7 years. In total, 19 patients (76%) are free of disease or of progression. Transplantation of highly purified peripheral-blood CD34(+) stem cells is associated with low toxicity in patients with nonmalignant diseases.
Lang
P
P
Children's University Hospital, Tübingen, Germany.
Klingebiel
T
T
Bader
P
P
Greil
J
J
Schumm
M
M
Schlegel
P-G
PG
Eyrich
M
M
Mueller-Weihrich
S
S
Niethammer
D
D
Handgretinger
R
R
D000293:Adolescent; D000740:Anemia / Q000401:mortality / Q000628:therapy*; D018952:Antigens, CD34; D001782:Blood Donors; D002648:Child; D002675:Child, Preschool; D006085:Graft Survival; D006086:Graft vs Host Disease; D006801:Humans; D007107:Immune System / Q000502:physiology; D007223:Infant; D008212:Lymphocyte Depletion; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D012038:Regeneration; D016019:Survival Analysis; D014184:Transplantation, Homologous
D018952:Antigens, CD34