Flow cytometry and T-cell response monitoring after smallpox vaccination.
Orthopoxvirus zoonosis or smallpox as result of bioterrorism or biological warfare represents a risk for epidemic spread. By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox. When the high similarity between the orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures.
Poccia
Fabrizio
F
National Institute for Infectious Diseases "Lazzaro Spallanzani," Rome, Italy. poccia@inmi.it
Gioia
Cristiana
C
Montesano
Carla
C
Martini
Federico
F
Horejsh
Douglas
D
Castilletti
Concetta
C
Pucillo
Leopoldo paolo
Lp
Capobianchi
Maria Rosaria
MR
Ippolito
Giuseppe
G
D000956:Antigens, Viral / Q000302:isolation & purification*; D005434:Flow Cytometry; D006801:Humans; D012899:Smallpox / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D012900:Smallpox Vaccine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000956:Antigens, Viral; D012900:Smallpox Vaccine