Microemulsion electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine.
A selective and sensitive microemulsion electrokinetic chromatography with laser-induced fluorescence detection method was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. By a series of optimization, a running buffer composed of 20 mM borate + microemulsion (23.3 mM Sodium dodecyl sulfate/180.85 mM 1-butanol/16.4 mM n-heptane) +8% acetonitrile was applied for the separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.058-11.58 microg.mL(-1) (correlation coefficient: 0.9993 for E, 0.9995 for PE), and the detection limits for E and PE were 5.3 and 3.9 ng.mL(-1). The method was applied to the analysis of the two alkaloids in Chinese traditional herbal preparations with recoveries in the range of 96.9-105.4%.
Zhang
Jiyou
J
Department of Chemistry, Lanzhou University, Lanzhou, China.
Xie
Jianping
J
Liu
Jiaqin
J
Tian
Jianniao
J
Chen
Xingguo
X
Hu
Zhide
Z
D000470:Alkaloids / Q000032:analysis; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D004365:Drugs, Chinese Herbal / Q000032:analysis; D004655:Emulsions; D004809:Ephedrine / Q000032:analysis*; D005453:Fluorescence; D007834:Lasers; D012680:Sensitivity and Specificity
D000470:Alkaloids; D002021:Buffers; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D004809:Ephedrine