title: [Mitochondrial DNA polymorphism reveals a genetic differentiation between ethnic groups in the population of Jerba].
abstract: Jerba is an island situated in the South-East of Tunisia were some ethnic groups (Arabs, Berbers, Blacks, Jewishs and others) cohabit for centuries. The religion and cultural differences have represented an obstacle to a mixture between these groups. In order to evaluate the genetic differentiation between the muslim groups (Arabs, Berbers and Blacks), we have analysed the polymorphism of a mitochondrial DNA coding region. The cytochrome oxydase coding region (COII) was amplified by PCR in 57 Arabs, 42 Berbers and 16 Blacks. The amplified products were analysed by Restriction Fragment Length Polymorphism (RFLP). Genetic distances were calculated by using the AMOVA program. The values of these distances were significantly different between Arabs and Blacks, and between Berbers and Blacks but not between Arabs and Berbers. So That, to refine the evaluation of genetic diversity between Arabs and Berbers, we have analysed the polymorphism of a second mitochondrial coding region which encodes for the fifth unit of NADH deshydrogenase (ND5). Eleven haplotypes were defined from the resulting data of mitochondrial COII and ND5 polymorphism and a significant genetic distance between Arabs and Berbers was computed.
authors.affiliation: Laboratoire d'Anthropologie Biologique, CNRS, Musée de l'Homme Paris.
authors.forename: B
authors.initials: B
authors.lastname: Yacoubi Loueslati
authors.forename: H
authors.initials: H
authors.lastname: Ennafaa
authors.forename: M
authors.initials: M
authors.lastname: Ben Amor
authors.forename: E
authors.initials: E
authors.lastname: Heyer
authors.forename: A
authors.initials: A
authors.lastname: Langaney
authors.forename: F
authors.initials: F
authors.lastname: Ben Ayed
authors.forename: A
authors.initials: A
authors.lastname: Ben Ammar Elgaaied
mesh_terms: D000704:Analysis of Variance; D018912:Arabs / Q000235:genetics*; D044383:Black People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007514:Islam; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D014416:Tunisia
chemical_list: D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV