title: Transformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans.
abstract: Transformation systems for Aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of Aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrG) of Aspergillus nidulans. An A. aculeatus mutant which can be transformed effectively by the A. nidulans pyrG gene was isolated as a transformation host. This is the first report of transformation of A. aculeatus.
authors.affiliation: Graduate School of Agriculture and Biological Sciences, and Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Kanamasa
authors.forename: Kana
authors.initials: K
authors.lastname: Yamaoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Sumitani
authors.forename: Motoo
authors.initials: M
authors.lastname: Arai
mesh_terms: D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D001236:Aspergillus oryzae / Q000235:genetics*; D015139:Blotting, Southern; D005822:Genetic Vectors; D009154:Mutation; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D011523:Protoplasts / Q000166:cytology; D011745:Pyrithiamine / Q000737:chemistry; D014170:Transformation, Genetic
chemical_list: D011745:Pyrithiamine; D009964:Orotidine-5'-Phosphate Decarboxylase