[The quantity analysis of reverse transcriptase in porcine endogenous retrovirus expressed in Banna minipig inbred].
Quantitative RT(reverse transcriptase) assay was established to detect the reverse transcriptase in plasma of thirty-four Chinese Banna minipig inbred in this work. The protocol was given in the RT kit (Roche), using HIV-1 as the positive control of the kit and supernatant of PK-15 as the PERV positive control respectively. The results show that positive reverse transcriptase reaction can be detected in the plasma of the pigs, but the levels are much lower than that of HIV-1 and lower than that of PERV in supernatant of PK-15.
Li
Shengfu
S
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital of Sichuan University, Chengdu 610041.
Yu
Ping
P
Zhang
Li
L
Bu
Hong
H
Li
Youping
Y
Zeng
Yangzhi
Y
D000818:Animals; D017849:Animals, Inbred Strains; D020077:Endogenous Retroviruses / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000097:blood*; D013552:Swine / Q000097:blood / Q000821:virology; D013556:Swine, Miniature / Q000097:blood / Q000821:virology*
D012194:RNA-Directed DNA Polymerase