Structural and functional characterization of pi bulges and other short intrahelical deformations.
We data-mined the Protein Data Bank for short intrahelical deformations, including pi bulges. These are defined as a contiguous stretch of intrahelical residues deviating from the standard alpha-helical i-->i-4 hydrogen bonding pattern, bilaterally flanked by at least one alpha-helical turn resulting in a helix kink of less than 40 degrees. We find that such motifs exist in 4.7% of a PDB subset filtered by quality metrics (resolution <2.5 A, R-factor <0.25, sequence identity <35%). These are typically characterized by at least one i-->i-5 main chain hydrogen bond, with energetically favorable main chain dihedral angles, followed by a variable number of main chain carbonyl groups that do not accept intrahelical main chain hydrogen bonds. Their stabilization commonly occurs via hydrogen bonding to water molecules or polar groups. Numerous deformations are implicated in basic yet vital functional roles, commonly as ligand binding site contributors.
Cartailler
Jean-Philippe
JP
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.
Luecke
Hartmut
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D018949:Antigens, CD1 / Q000378:metabolism; D001436:Bacteriorhodopsins / Q000378:metabolism; D030562:Databases, Protein; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D011487:Protein Conformation; D013314:Stress, Mechanical
D018949:Antigens, CD1; C419394:CD1b antigen; D010716:Phosphatidylinositols; D001436:Bacteriorhodopsins