title: Laser desorption combined with hyperthermal surface ionization time-of-flight mass spectrometry.
abstract: A setup combining laser desorption of nonvolatile molecules and their aerodynamic acceleration in a supersonic molecular beam followed by hyperthermal surface ionization in a reflectron time-of-flight mass spectrometer is described. While laser desorption performs the intact transfer of the analyte molecules into the gas phase, hyperthermal surface ionization opens up the possibility to efficiently ionize even larger molecules with a small and potentially controlled degree of fragmentation. Being an ionization technique, which is particularly effective for aromatic and heterocyclic compounds, the selectivity can further be increased by tuning the kinetic energy to which the molecules are accelerated in the supersonic beam. The results obtained for several polycyclic aromatic hydrocarbons and biochemical substances show that sufficient acceleration can be achieved even for molecules with a molecular weight above 5000 amu and that HSI preserves its advantageous features even for thermally labile large molecules such as insulin.
authors.affiliation: Department of Computer Science, Mathematics and Natural Sciences, Leipzig University of Applied Sciences, Trufanow Strasse 6, 04105 Leipzig, Germany. p1weick@nawi.htwk-leipzig.de
authors.forename: Christian
authors.initials: C
authors.lastname: Weickhardt
authors.forename: Lars
authors.initials: L
authors.lastname: Draack
authors.forename: Aviv
authors.initials: A
authors.lastname: Amirav