Phosphotyrosine phosphoepitopes can be rapidly analyzed by coexpression of a tyrosine kinase in bacteria with a T7 bacteriophage display library.
Khati
M
M
Department of Metabolic Medicine, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.
Pillay
T S
TS
D000818:Animals; D000906:Antibodies; D019677:Artificial Gene Fusion; D001419:Bacteria / Q000235:genetics*; D017123:Bacteriophage T7 / Q000235:genetics*; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D019151:Peptide Library; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
D000906:Antibodies; D000939:Epitopes; D019151:Peptide Library; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases