title: Applications of laser-polarized 129Xe to biomolecular assays.
abstract: The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and functionalized 129Xe NMR to in vitro biomolecular assays suggests the potential future use of a functionalized xenon biosensor for in vivo imaging.
authors.affiliation: Department of Chemistry, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Lowery
authors.forename: Seth M
authors.initials: SM
authors.lastname: Rubin
authors.forename: E Janette
authors.initials: EJ
authors.lastname: Ruiz
authors.forename: Megan M
authors.initials: MM
authors.lastname: Spence
authors.forename: Nicolas
authors.initials: N
authors.lastname: Winssinger
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
authors.forename: Alexander
authors.initials: A
authors.lastname: Pines
authors.forename: David E
authors.initials: DE
authors.lastname: Wemmer
mesh_terms: D006801:Humans; D007834:Lasers; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011487:Protein Conformation; D014979:Xenon Isotopes
chemical_list: D008024:Ligands; D014979:Xenon Isotopes