title: Structural analysis of sphingophospholipids derived from Sphingobacterium spiritivorum, the type species of genus Sphingobacterium.
abstract: The unique feature of the genus Sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. As major cellular lipid components, five kinds of sphingophospholipids were purified from Sphingobacterium spiritivorum ATCC 33861(T), the type species of genus Sphingobacterium. They were identified as ceramide phosphorylethanolamines (CerPE-1 and CerPE-2), ceramide phosphoryl-myo-inositols (CerPI-1 and CerPI-2), and ceramide phosphorylmannose (CerPM-1). The ceramide of CerPE-1, CerPI-1, and CerPM-1 was composed of 15-methylhexadecasphinganine (isoheptadeca sphinganine, iso-C17:0) and 13-methyltetradecanoic acid (isopentadecanoic acid, iso-C15:0), whereas that of CerPE-2 and CerPI-2 was composed of isoheptadeca sphinganine and 2-hydroxy-13-methyltetradecanoic acid (2-hydroxy isopentadecanoic acid, 2-OH iso-C15:0). These sphingophospholipids were also found in cellular lipids of Sphingobacterium multivorum ATCC 33613(T), Sphingobacterium mizutaii ATCC 33299(T), Sphingobacterium faecium IFO 15299(T), Sphingobacterium thalpophilum ATCC 43320(T), and Sphingobacterium antarcticum ATCC 51969(T). To our knowledge, the existence of CerPM-1 is a novel sphingophospholipid through eukaryotic and prokaryotic cells.
authors.affiliation: Department of Host Defense, Osaka City University Graduate School of Medicine, Japan. tnaka@med.osaka-cu.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Naka
authors.forename: Nagatoshi
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Ikuya
authors.initials: I
authors.lastname: Yano
authors.forename: Shinji
authors.initials: S
authors.lastname: Maeda
authors.forename: Matsumi
authors.initials: M
authors.lastname: Doe
authors.forename: Miki
authors.initials: M
authors.lastname: Minamino
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ikeda
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kato
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Watabe
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: Ikuko
authors.initials: I
authors.lastname: Tomiyasu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D002518:Ceramides / Q000032:analysis; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D045249:Sphingobacterium / Q000737:chemistry*; D013107:Sphingolipids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D002518:Ceramides; D005227:Fatty Acids; D010743:Phospholipids; D013107:Sphingolipids; C047140:isopentadecanoic acid