Detection of a set of peptide vaccine candidates for use in HLA-A31+ epithelial cancer patients.
The molecular basis of host-tumor interaction in HLA-A31+ cancer patients has not been well understood. This lack of clarification is hampering the development of specific immunotherapies for these patients. This study aimed to identify a set of CTL-epitope peptides applicable for the specific immunotherapy of cancer patients with HLA-A31 allele. HLA-A31 allele is expressed in 5-10% of the world population, with the highest expression among Brazilian Amerinds (65%), and the lowest in the Eskimo population (0%). We report herein four cDNAs encoding CTL-epitopes and 7 epitope peptides with the ability to induce HLA-A31-restricted CTLs cytotoxic to tumor cell lines in the peripheral blood mononuclear cells of HLA-A31+ cancer patients. These peptides might be useful for the development of a peptide-based immunotherapy for HLA-A31+ cancer patients.
Takedatsu
Hiroko
H
Department of Immunology, Kurume University School of Medicine, Kurume, Japan.
Shichijo
Shigeki
S
Azuma
Kouichi
K
Takedatsu
Hidetoshi
H
Sata
Michio
M
Itoh
Kyogo
K
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D015152:Blotting, Northern; D019556:COS Cells; D019496:Cancer Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000939:Epitopes / Q000737:chemistry; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008969:Molecular Sequence Data; D009375:Neoplasms, Glandular and Epithelial / Q000208:ethnology / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism; D022223:Vaccines, Subunit / Q000737:chemistry*
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018076:DNA, Complementary; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C069527:HLA-A31 antigen; D010455:Peptides; D012333:RNA, Messenger; D022223:Vaccines, Subunit