title: Impaired neuromuscular transmission and skeletal muscle fiber necrosis in mice lacking Na/Ca exchanger 3.
abstract: We produced and analyzed mice deficient for Na/Ca exchanger 3 (NCX3), a protein that mediates cellular Ca(2+) efflux (forward mode) or Ca(2+) influx (reverse mode) and thus controls intracellular Ca(2+) concentration. NCX3-deficient mice (Ncx3(-/-)) present a skeletal muscle fiber necrosis and a defective neuromuscular transmission, reflecting the absence of NCX3 in the sarcolemma of the muscle fibers and at the neuromuscular junction. The defective neuromuscular transmission is characterized by the presence of electromyographic abnormalities, including low compound muscle action potential amplitude, a decremental response at low-frequency nerve stimulation, an incremental response, and a prominent postexercise facilitation at high-frequency nerve stimulation, as well as neuromuscular blocks. The analysis of quantal transmitter release in Ncx3(-/-) neuromuscular junctions revealed an important facilitation superimposed on the depression of synaptic responses and an elevated delayed release during high-frequency nerve stimulation. It is suggested that Ca(2+) entering nerve terminals is cleared relatively slowly in the absence of NCX3, thereby enhancing residual Ca(2+) and evoked and delayed quantal transmitter release during repetitive nerve stimulation. Our findings indicate that NCX3 plays an important role in vivo in the control of Ca(2+) concentrations in the skeletal muscle fibers and at the neuromuscular junction.
authors.affiliation: Institut de Recherches en Biologie Humaine et Moléculaire-Institut de Biologie et de Médecine Moléculaires, and Laboratory of Pharmacology and Therapeutics, Université Libre de Bruxelles, Gosselies, Belgium. ssokolow@ulb.ac.be
authors.forename: Sophie
authors.initials: S
authors.lastname: Sokolow
authors.forename: Mario
authors.initials: M
authors.lastname: Manto
authors.forename: Philippe
authors.initials: P
authors.lastname: Gailly
authors.forename: Jordi
authors.initials: J
authors.lastname: Molgó
authors.forename: Clarisse
authors.initials: C
authors.lastname: Vandebrouck
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Vanderwinden
authors.forename: Andre
authors.initials: A
authors.lastname: Herchuelz
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schurmans
mesh_terms: D000483:Alleles; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004576:Electromyography; D005070:Evans Blue / Q000494:pharmacology; D005091:Exons; D015151:Immunoblotting; D026901:Membrane Transport Proteins; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000473:pathology*; D009336:Necrosis; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012508:Sarcolemma / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000235:genetics* / Q000502:physiology*; D009435:Synaptic Transmission; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D026901:Membrane Transport Proteins; C491101:Slc8a3 protein, mouse; D019831:Sodium-Calcium Exchanger; D005070:Evans Blue; D012313:RNA; D002118:Calcium