Immobilization of Pseudomonas delafieldii with magnetic polyvinyl alcohol beads and its application in biodesulfurization.
Pseudomonas delafieldii was immobilized in magnetic polyvinyl alcohol (PVA) beads using a hydrophilic magnetic fluid, which was prepared by a co-precipitation method. The beads had distinct super-paramagnetic properties and were compared with immobilized cells in non-magnetic PVA beads. Their desulfurizing activity was increased slightly from 8.7 to 9 mmol sulfur kg(-1) (dry cell) h(-1). The main advantages was that the magnetic immobilized cells maintain a high desulfurization activity and remain in good shape after 7 times of repeated use, while the non-magnetic immobilized cells could only be used for 5 times. Furthermore, the magnetic immobilized cells could be easily collected or separated magnetically from the biodesulfurization reactor.
Shan
Guo-Bin
GB
Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
Xing
Jian-Min
JM
Luo
Ming-Fang
MF
Liu
Hui-Zhou
HZ
Chen
Jia-Yong
JY
D001422:Bacterial Adhesion / Q000502:physiology*; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D008280:Magnetics; D008863:Microspheres; D011142:Polyvinyl Alcohol; D011549:Pseudomonas / Q000302:isolation & purification / Q000502:physiology*; D013455:Sulfur / Q000378:metabolism*
D020099:Coated Materials, Biocompatible; D013455:Sulfur; D011142:Polyvinyl Alcohol