title: Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica.
abstract: Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.
authors.affiliation: Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, 305-8687 Japan. iga@ffpri.affrc.go.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Igasaki
authors.forename: Noriko
authors.initials: N
authors.lastname: Akashi
authors.forename: Tokuko
authors.initials: T
authors.lastname: Ujino-Ihara
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Matsubayashi
authors.forename: Youji
authors.initials: Y
authors.lastname: Sakagami
authors.forename: Kenji
authors.initials: K
authors.lastname: Shinohara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D032485:Cryptomeria / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D036361:Peptide Hormones; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D018076:DNA, Complementary; C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011092:Polyethylene Glycols