title: Lysophosphatidic acid stimulates calcium transients in enteric glia.
abstract: The enteric nervous system plays an integral role in the gastrointestinal tract. Within this intricate network, enteric glia are crucial in the maintenance of normal bowel function, yet their signaling mechanisms are poorly understood. Enteric glia, and not enteric neurons, selectively responded to lysophosphatidic acid (LPA), a product of phosphatidylcholine metabolism, with dose-dependent calcium (Ca(2+)) signaling over a range from 100 pM to 10 microM. The elicited calcium transients involved both the mobilization of intracellular Ca(2+) stores and the influx of extracellular Ca(2+) as LPA signals were obliterated following the depletion of intracellular Ca(2+) and attenuated by the removal of Ca(2+) from the perfusion buffer. Pretreatment with pertussis toxin (100 ng/ml) reduced the magnitude of LPA Ca(2+) transients (95+/-20 nM vs 168+/-17 nM for controls). Repetitive exposure yielded diminished responsiveness, with a 25% reduction in [Ca(2+)](i) between first and second exposures. Inhibition of the inositol 1,4,5-trisphosphate (IP(3)) receptor with 200 microM 2-aminoethoxydiphenylborate (2APB) abolished LPA signals. RT-PCR analysis demonstrated the presence of two LPA-coupled endothelial differentiation gene (EDG) receptor mRNAs (EDG-2 and EDG-7) in myenteric plexus primary cultures. EDG-2 expression in glial cells of the ENS was confirmed immunocytochemically.
authors.affiliation: Department of Surgery, University of Michigan, 2101 Taubman Center, 1500 East Medical Center Drive, 48109-0346, Ann Arbor, MI, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Segura
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: R A
authors.initials: RA
authors.lastname: Cowles
authors.forename: L
authors.initials: L
authors.lastname: Xiao
authors.forename: T R
authors.initials: TR
authors.lastname: Lin
authors.forename: C
authors.initials: C
authors.lastname: Logsdon
authors.forename: M W
authors.initials: MW
authors.lastname: Mulholland
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017615:Enteric Nervous System / Q000187:drug effects* / Q000378:metabolism; D006168:Guinea Pigs; D008246:Lysophospholipids / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D049312:Receptors, Lysophospholipid
chemical_list: D008246:Lysophospholipids; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid