title: AmSUT1, a sucrose transporter in collection and transport phloem of the putative symplastic phloem loader Alonsoa meridionalis.
abstract: A sucrose (Suc) transporter cDNA has been cloned from Alonsoa meridionalis, a member of the Scrophulariaceae. This plant species has an open minor vein configuration and translocates mainly raffinose and stachyose in addition to Suc in the phloem (C. Knop, O. Voitsekhovskaja, G. Lohaus [2001] Planta 213: 80-91). These are typical properties of symplastic phloem loaders. For functional characterization, AmSUT1 cDNA was expressed in bakers' yeast (Saccharomyces cerevisiae). Substrate and inhibitor specificities, energy dependence, and Km value of the protein agree well with the properties measured for other Suc transporters of apoplastic phloem loaders. A polyclonal antiserum against the 17 N-terminal amino acids of the A. meridionalis Suc transporter AmSUT1 was used to determine the cellular localization of the AmSUT1 protein. Using fluorescence labeling on sections from A. meridionalis leaves and stems, AmSUT1 was localized exclusively in phloem cells. Further histological characterization identified these cells as companion cells and sieve elements. p-Chloromercuribenzenesulfonic acid affected the sugar exudation of cut leaves in such a way that the exudation rates of Suc and hexoses decreased, whereas those of raffinose and stachyose increased. The data presented indicate that phloem loading of Suc and retrieval of Suc in A. meridionalis are at least partly mediated by the activity of AmSUT1 in addition to symplastic phloem loading.
authors.affiliation: Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Abteilung Biochemie der Pflanze, Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Knop
authors.forename: Ruth
authors.initials: R
authors.lastname: Stadler
authors.forename: Norbert
authors.initials: N
authors.lastname: Sauer
authors.forename: Gertrud
authors.initials: G
authors.lastname: Lohaus
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004274:DNA, Recombinant / Q000235:genetics; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D028083:Scrophulariaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D004274:DNA, Recombinant; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D013439:Sulfhydryl Reagents; C078729:sucrose transport protein, plant; D002731:4-Chloromercuribenzenesulfonate