Some properties of human small heat shock protein Hsp20 (HspB6).
Human heat shock protein of apparent molecular mass 20 kDa (Hsp20) and its mutant, S16D, mimicking phosphorylation by cyclic nucleotide-dependent protein kinases, were cloned and expressed in Escherichia coli. The proteins were obtained in a homogeneous state without utilization of urea or detergents. On size exclusion chromatography at neutral pH, Hsp20 and its S16D mutant were eluted as symmetrical peaks with an apparent molecular mass of 55-60 kDa. Chemical crosslinking resulted in the formation of dimers with an apparent molecular mass of 42 kDa. At pH 6.0, Hsp20 and its S16D mutant dissociated, and were eluted in the form of two peaks with apparent molecular mass values of 45-50 and 28-30 kDa. At pH 7.0-7.5, the chaperone activity of Hsp20 (measured by its ability to prevent the reduction-induced aggregation of insulin or heat-induced aggregation of yeast alcohol dehydrogenase) was similar to or higher than that of commercial alpha-crystallin. Under these conditions, the S16D mutant of Hsp20 possessed lower chaperone activity than the wild-type protein. At pH 6.0, both alpha-crystallin and Hsp20 interacted with denatured alcohol dehydrogenase; however, alpha-crystallin prevented, whereas Hsp20 either did not affect or promoted, the heat-induced aggregation of alcohol dehydrogenase. The mixing of wild-type human Hsp27 and Hsp20 resulted in a slow, temperature-dependent formation of hetero-oligomeric complexes, with apparent molecular mass values of 100 and 300 kDa, which contained approximately equal amounts of Hsp27 and Hsp20 subunits. Phosphorylation of Hsp27 by mitogen activated protein kinase-activated protein kinase 2 was mimicked by replacing Ser15, 78 and 82 with Asp. A 3D mutant of Hsp27 mixed with Hsp20 rapidly formed a hetero-oligomeric complex with an apparent molecular mass of 100 kDa, containing approximately equal quantities of two small heat shock proteins.
Bukach
Olesya V
OV
Department of Biochemistry, School of Biology, Moscow State University, Moscow, Russia.
Seit-Nebi
Alim S
AS
Marston
Steven B
SB
Gusev
Nikolai B
NB
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D019281:Dimerization; D004789:Enzyme Activation; D004926:Escherichia coli / Q000378:metabolism; D050886:HSP20 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D007328:Insulin / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D010766:Phosphorylation; D011489:Protein Denaturation; D011494:Protein Kinases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D038201:alpha-Crystallins / Q000378:metabolism
D003432:Cross-Linking Reagents; D050886:HSP20 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; C494382:HSPB6 protein, human; D006360:Heat-Shock Proteins; D007328:Insulin; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D038201:alpha-Crystallins; D000426:Alcohol Dehydrogenase; D011494:Protein Kinases; D020928:Mitogen-Activated Protein Kinases