[Neuroprotective effect of monoamine oxidase monoclonal antibody against vasogenic brain edema in rats].
OBJECTIVE
To observe the therapeutic effect of monoamine oxidase (MAO) monoclonal antibody (mAb) for vasogenic brain edema (VBE) in rats.


METHODS
A total of 75 Wistar rats were randomized into non-edema, non-treated edema, saline-treated edema, mannitol-treated edema and MAO mAb-treated groups. Rat models of VBE were established by intraperitoneal injection of phenylephrine in the latter 4 groups. Brain water content in the gray and white matter was measured respectively with a moisture analyzer, and the permeability of the blood-brain barrier (BBB) determined by Evan's blue (EB) extravasation method.


RESULTS
MAO mAb administration significantly reduced the brain water content in the gray and white matter as well as the permeability of BBB (P<0.01), Which was especially effective for the white matter, producing results comparable with those of the non-edema group (P>0.05). MAO mAb markedly alleviated brain edema, with better dehydrating effect on the white matter than mannitol (P<0.01), which reduced the water content of the brain gray and white matter undiscriminatingly and showed poor effect on the permeability of BBB.


CONCLUSIONS
The pathogenesis of BBB permeability changes in VBE is related to the activity of monoamine oxidase, and MAO mAb has selective therapeutic effect on VBE, which is especially obvious on brain white matter.
Li
Qiang
Q
Department of Neurology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Lu
Bing-xun
BX
Wang
Xin-yu
XY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001812:Blood-Brain Barrier; D001929:Brain Edema / Q000378:metabolism / Q000628:therapy*; D002199:Capillary Permeability; D008297:Male; D008995:Monoamine Oxidase / Q000276:immunology*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D018696:Neuroprotective Agents; D008995:Monoamine Oxidase