Complete sequencing and characterization of 21,243 full-length human cDNAs.
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.
Ota
Toshio
T
Helix Research Institute, 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan.
Suzuki
Yutaka
Y
Nishikawa
Tetsuo
T
Otsuki
Tetsuji
T
Sugiyama
Tomoyasu
T
Irie
Ryotaro
R
Wakamatsu
Ai
A
Hayashi
Koji
K
Sato
Hiroyuki
H
Nagai
Keiichi
K
Kimura
Kouichi
K
Makita
Hiroshi
H
Sekine
Mitsuo
M
Obayashi
Masaya
M
Nishi
Tatsunari
T
Shibahara
Toshikazu
T
Tanaka
Toshihiro
T
Ishii
Shizuko
S
Yamamoto
Jun-ichi
J
Saito
Kaoru
K
Kawai
Yuri
Y
Isono
Yuko
Y
Nakamura
Yoshitaka
Y
Nagahari
Kenji
K
Murakami
Katsuhiko
K
Yasuda
Tomohiro
T
Iwayanagi
Takao
T
Wagatsuma
Masako
M
Shiratori
Akiko
A
Sudo
Hiroaki
H
Hosoiri
Takehiko
T
Kaku
Yoshiko
Y
Kodaira
Hiroyo
H
Kondo
Hiroshi
H
Sugawara
Masanori
M
Takahashi
Makiko
M
Kanda
Katsuhiro
K
Yokoi
Takahide
T
Furuya
Takako
T
Kikkawa
Emiko
E
Omura
Yuhi
Y
Abe
Kumi
K
Kamihara
Kumiko
K
Katsuta
Naoko
N
Sato
Kazuomi
K
Tanikawa
Machiko
M
Yamazaki
Makoto
M
Ninomiya
Ken
K
Ishibashi
Tadashi
T
Yamashita
Hiromichi
H
Murakawa
Katsuji
K
Fujimori
Kiyoshi
K
Tanai
Hiroyuki
H
Kimata
Manabu
M
Watanabe
Motoji
M
Hiraoka
Susumu
S
Chiba
Yoshiyuki
Y
Ishida
Shinichi
S
Ono
Yukio
Y
Takiguchi
Sumiyo
S
Watanabe
Susumu
S
Yosida
Makoto
M
Hotuta
Tomoko
T
Kusano
Junko
J
Kanehori
Keiichi
K
Takahashi-Fujii
Asako
A
Hara
Hiroto
H
Tanase
Tomo-o
TO
Nomura
Yoshiko
Y
Togiya
Sakae
S
Komai
Fukuyo
F
Hara
Reiko
R
Takeuchi
Kazuha
K
Arita
Miho
M
Imose
Nobuyuki
N
Musashino
Kaoru
K
Yuuki
Hisatsugu
H
Oshima
Atsushi
A
Sasaki
Naokazu
N
Aotsuka
Satoshi
S
Yoshikawa
Yoko
Y
Matsunawa
Hiroshi
H
Ichihara
Tatsuo
T
Shiohata
Namiko
N
Sano
Sanae
S
Moriya
Shogo
S
Momiyama
Hiroko
H
Satoh
Noriko
N
Takami
Sachiko
S
Terashima
Yuko
Y
Suzuki
Osamu
O
Nakagawa
Satoshi
S
Senoh
Akihiro
A
Mizoguchi
Hiroshi
H
Goto
Yoshihiro
Y
Shimizu
Fumio
F
Wakebe
Hirokazu
H
Hishigaki
Haretsugu
H
Watanabe
Takeshi
T
Sugiyama
Akio
A
Takemoto
Makoto
M
Kawakami
Bunsei
B
Yamazaki
Masaaki
M
Watanabe
Koji
K
Kumagai
Ayako
A
Itakura
Shoko
S
Fukuzumi
Yasuhito
Y
Fujimori
Yoshifumi
Y
Komiyama
Megumi
M
Tashiro
Hiroyuki
H
Tanigami
Akira
A
Fujiwara
Tsutomu
T
Ono
Toshihide
T
Yamada
Katsue
K
Fujii
Yuka
Y
Ozaki
Kouichi
K
Hirao
Maasa
M
Ohmori
Yoshihiro
Y
Kawabata
Ayako
A
Hikiji
Takeshi
T
Kobatake
Naoko
N
Inagaki
Hiromi
H
Ikema
Yasuko
Y
Okamoto
Sachiko
S
Okitani
Rie
R
Kawakami
Takuma
T
Noguchi
Saori
S
Itoh
Tomoko
T
Shigeta
Keiko
K
Senba
Tadashi
T
Matsumura
Kyoka
K
Nakajima
Yoshie
Y
Mizuno
Takae
T
Morinaga
Misato
M
Sasaki
Masahide
M
Togashi
Takushi
T
Oyama
Masaaki
M
Hata
Hiroko
H
Watanabe
Manabu
M
Komatsu
Takami
T
Mizushima-Sugano
Junko
J
Satoh
Tadashi
T
Shirai
Yuko
Y
Takahashi
Yukiko
Y
Nakagawa
Kiyomi
K
Okumura
Koji
K
Nagase
Takahiro
T
Nomura
Nobuo
N
Kikuchi
Hisashi
H
Masuho
Yasuhiko
Y
Yamashita
Riu
R
Nakai
Kenta
K
Yada
Tetsushi
T
Nakamura
Yusuke
Y
Ohara
Osamu
O
Isogai
Takao
T
Sugano
Sumio
S
D002904:Chromosomes, Human, 21-22 and Y; D002890:Chromosomes, Human, Pair 20; D019295:Computational Biology; D018076:DNA, Complementary; D006801:Humans; D016366:Open Reading Frames; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger