title: Mass spectrometric phenotyping of Val34Leu polymorphism of blood coagulation factor XIII by differential peptide display.
abstract: BACKGROUND
  The Val34Leu mutation in the activation peptide of factor XIII (FXIIIA) correlates with a lower incidence of myocardial infarction and ischemic stroke but an increased risk for hemorrhagic stroke. We describe mass spectrometric detection of the activation peptide variants in human serum.
  
  
  METHODS
  We used differential peptide display (DPD) to compare comprehensive peptide maps from pairs of serum samples from healthy volunteers. Peptides were separated by liquid chromatography, and fractions were subjected to mass spectrometry. Mass spectra of all fractions were combined, giving a peptide map representing a two-dimensional display of peptide masses. After comparison of peptide mass maps, peptides that differentiated FXIIIA phenotypes were identified by mass spectrometry.
  
  
  RESULTS
  Val34Leu polymorphisms of the activation peptide of FXIIIA were identified in 20 serum samples from 10 volunteers by DPD, and their sequences were confirmed by nanoelectrospray-ionization quadrupole time-of-flight mass spectrometry. Analysis of three (V34V, V34L, and L34L) phenotypes was confirmed by allele-specific genotypic analysis in all (n = 10) volunteers.
  
  
  CONCLUSIONS
  DPD provides a simple and easy-to-use phenotype assay with advantages over PCR-based assays in being faster and directly analyzing the compound of interest.
authors.affiliation: BioVisioN AG, Hannover, Germany. h.tammen@biovision-discovery.de
authors.forename: Harald
authors.initials: H
authors.lastname: Tammen
authors.forename: Thomas
authors.initials: T
authors.lastname: Möhring
authors.forename: Markus
authors.initials: M
authors.lastname: Kellmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Pich
authors.forename: Hans H
authors.initials: HH
authors.lastname: Kreipe
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Hess
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005169:Factor VIII / Q000235:genetics*; D006801:Humans; D007930:Leucine / Q000235:genetics*; D008297:Male; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000097:blood*; D010641:Phenotype; D011110:Polymorphism, Genetic; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014633:Valine / Q000235:genetics*
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D005169:Factor VIII; D007930:Leucine; D014633:Valine