title: The insulator effect of the 5'HS4 region from the beta-globin chicken locus on the rabbit WAP gene promoter activity in transgenic mice.
abstract: Previous studies have shown that the 5'HS4 DNaseI hypersensitive site of the chicken beta-globin locus is endowed with classic insulator activities: (i) it blocks the interaction between promoter and enhancers when it is inserted between them (ii) it confers expression of integrated foreign genes independent of their position in the chromatin. The aim of this present work was to determine whether the 5'HS4 element was able to stimulate the expression level and/or to increase the expression frequency of a luc+ reporter gene controlled by the rabbit WAP gene promoter. Two constructs with 5'HS4 insulator (p5'HS4-WAPluc) or without (pWAPluc) were introduced in mouse fertilised oocytes. All transgenic lines containing the 5'HS4 element (six lines) expressed the transgene whereas only two out of eight lines harbouring the pWAP-luc construct expressed the transgene to a significant level. Moreover, the mean level of expression was seven times higher in p5'HS4WAP-luc lines than in pWAP-luc lines. Even all these benefits on transgene expression, the 5'HS4 element did not confer a copy-dependent expression, did not decrease the ectopic expression of the reporter gene and did not decrease the variability of expression. Thus, the 5'HS4 element does not have all the properties of a perfect insulator on a construct containing the luc+ reporter gene controlled by the rabbit WAP promoter.
authors.affiliation: Unité de Biologie du Développement et Biotechnologies, Institut National de la Recherche Agronomique, F-78352 Jouyv-en-Josas, France. rival@jouy.inra.fr
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rival-Gervier
authors.forename: Thais
authors.initials: T
authors.lastname: Pantano
authors.forename: Céline
authors.initials: C
authors.lastname: Viglietta
authors.forename: Caroline
authors.initials: C
authors.lastname: Maeder
authors.forename: Sonia
authors.initials: S
authors.lastname: Prince
authors.forename: Joe
authors.initials: J
authors.lastname: Attal
authors.forename: Geneviève
authors.initials: G
authors.lastname: Jolivet
authors.forename: Louis-Marie
authors.initials: LM
authors.lastname: Houdebine
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005914:Globins / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008894:Milk Proteins / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits
chemical_list: D008894:Milk Proteins; C024471:whey acidic proteins; D005914:Globins; D008156:Luciferases