[Study on DNA polymerase beta gene mutation in human cervical cancer].
OBJECTIVE
To investigate whether DNA polymerase beta (POLB) gene mutations occur in human cervical cancer (CC).


METHODS
To collect fresh specimens from 34 cervical cancer and examine the mutation of POLB gene using reverse transcription polymerase chain reaction (RT-PCR), single strand conformation polymorphism (SSCP) analysis and sequence analysis. According to their histological grading, the 34 cases of CC were divided into three groups: 9 cases of grade I (G(1)), 14 cases of grade II (G(2)), 11 cases of grade III (G(3)).


RESULTS
POLB mutations were detected in the tissues of CC. The mutation of POLB is related with the histological differentiated of the CC. The mutated rates in low differentiated cancer were significantly higher than those in moderate and high ones, and the difference was significant (P < 0.05). The sequencing of the PCR product showed an A to G chang at nucleotide 660, which resulted in a substitution of the amino acid from arginine to glycin at codon 182.


CONCLUSIONS
There were POLB mutations in the tissues of CC, and this may be related with the development of the CC. The mutations impaired the catalytic activity of POLB. As a result, this may lead to observed accumulation of mutations in tumor cells.
Han
Li-ping
LP
Department of Obstetrics and Gynecology, the First Affliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Qiao
Yu-huan
YH
Dong
Zi-ming
ZM
Shi
Hui-rong
HR
Zhao
Guo-qiang
GQ
Liu
Dong
D
D019951:DNA Polymerase beta / Q000235:genetics*; D005260:Female; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology
D019951:DNA Polymerase beta