title: Kinetic studies of the interaction between antitumor antibiotics and DNA using quartz crystal microbalance.
abstract: OBJECTIVE
  Kinetic studies of the interaction process between antitumor antibiotics, Mitomycin C (MMC) and Bleomycin (BLM), and DNA were performed with a novel analytical method, piezoelectric quartz crystal (PQC) impedance analysis.
  
  
  METHODS
  DNA was directly immobilized on the Au-electrode surface of a piezoelectric quartz crystal by adsorption. The DNA-modified piezoelectric sensor was in contact with Mitomycin C and Bleomycin solution, respectively.
  
  
  RESULTS
  The experimental results demonstrated that antitumor antibiotics concentration had an effect on the interaction. A pseudo-first-order kinetic model for the interaction between antitumor antibiotics and DNA was derived to describe the process. All fitted results were well in agreement with the corresponding experimental results. The kinetic parameters, the interaction rate constants (k(MMC) and k(BLM)), were determined by fitting experimental data to the model. At 37 +/- 0.5 degrees C, the k(MMC) and k(BLM) values obtained were 4.56 (+/-0.02) x 10(-3) and 9.11 (+/-0.02) mM(-1) s(-1), respectively.
  
  
  CONCLUSIONS
  Piezoelectric quartz crystal impedance (PQCI) analysis is a very powerful method to study the kinetic process of antitumor drugs and DNA interaction.
authors.affiliation: State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Tian
authors.forename: Wanzhi
authors.initials: W
authors.lastname: Wei
authors.forename: Youan
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D017097:Electric Impedance; D007700:Kinetics; D008956:Models, Chemical
chemical_list: D000970:Antineoplastic Agents; D004247:DNA