Analysis of the distribution and regulation of three representative subtilase genes in sapstaining fungi.
In order to grow in wood, sapstaining fungi produce multiple proteases. Previously we have shown that three groups of subtilases appear to be present in sapstaining fungi; however, it is unknown whether these groups have distinct physiological roles. A representative gene from each of the three groups was chosen and the copy number and presence of homologous genes in other sapstaining fungi were determined. As well, the expressional regulation of these genes was determined in response to available nutrients, exogenous pH, and culture age. Gene homologues in the Ofloc1 group were common in Ophiostoma species. However, homologues from the Opic group were found in only certain Ophiostoma species. Cr group homologues were found in all of the species tested, except for Ophiostoma piceae. The expression of opil1, an Ofloc1 group gene, was induced by BSA, regulated by pH, and expressed within 12h of induction by BSA. The expression of the opic gene, an Opic group gene, was induced by BSA but required the removal of either nitrogen or carbon repression, was also regulated by pH, and was expressed within 24h of BSA induction. The Cr group gene opil2 was expressed under all conditions tested.
Hoffman
Brad
B
Department of Wood Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
Breuil
Colette
C
D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013381:Subtilisins / Q000235:genetics* / Q000378:metabolism*
D004271:DNA, Fungal; D005656:Fungal Proteins; D000644:Quaternary Ammonium Compounds; D012331:RNA, Fungal; D012710:Serum Albumin, Bovine; D013381:Subtilisins; D005947:Glucose