[Arrangement of the sense and terminating codons of the template in the A-segment of human ribosomes from photocrosslinking data withe oligonucleotide derivatives].
Oligoribonucleotide derivatives containing Phe codon UUC along with a 3'-flanking sense codon or stop codon carrying a perfluoroarylazido group at G or U were used to study the position of each nucleotide of the latter codon relative to the 18S rRNA in the A site of the 80S ribosome. To place the modified sense or stop codon in the A site, UCC-recognizing tRNA(Phe) was bound in the P site. Regardless of the position in the sense or stop codon, the modified nucleotide crosslinked with invariant dinucleotide A1823/A1824 or nucleotide A1825 in helix 44 close to the 3' end of the 18S rRNA. Located in the second or third position of either codon, the modified G bound with invariant nucleotide G626, which is in the evolutionarily conserved 530 stem-loop segment. The results were collated with the X-ray structure of the bacterial ribosome, and the template codon was assumed to be similarly arranged relative to the small-subunit rRNA in various organisms.
Stiazhkina
V A
VA
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Molotkov
M V
MV
Demeshkina
N A
NA
Bulygin
K N
KN
Graĭfer
D M
DM
Meshchaninova
M I
MI
Repkova
M N
MN
Ven'iaminova
A G
AG
Karpova
G G
GG
D001483:Base Sequence; D018388:Codon, Terminator; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D010777:Photochemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D014961:X-Ray Diffraction
D018388:Codon, Terminator; D009843:Oligoribonucleotides; D012336:RNA, Ribosomal, 16S