title: Novel spirocyclic trichothecanes, spirotenuipesine A and B, isolated from entomopathogenic fungus, Paecilomyces tenuipes.
abstract: Entomopathogenic fungi forming fruiting bodies have been employed as tonics and antitussives from ancient times. Paecilomyces tenuipes, which is also called Isaria japonica, is a very popular entomopathogenic fungus and is often considered a health food in northeast Asian countries such as China, Korea, and Japan. We cultivated the fruiting bodies of Paecilomyces tenuipes. Among the large-scale cultivations, fruiting body grown in barley grain contained two novel spirocyclic trichothecane derivatives, spirotenuipesine A (1) and B (2), and known trichothecane mycotoxins. Compounds 1 and 2 showed potent activity in neurotrophic factor biosynthesis in glial cells. The isolation of these compounds indicated that P. tenuipes is a promising source for producing various biologically active substances including trichothecanes. It is noteworthy that trichothecane mycotoxins are present in Paecilomyces tenuipes, which is typically used in medicinal health food.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-yama, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kikuchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inatomi
authors.forename: Asami
authors.initials: A
authors.lastname: Haganuma
authors.forename: Norimichi
authors.initials: N
authors.lastname: Nakahata
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D006571:Heterocyclic Compounds / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010143:Paecilomyces / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D006571:Heterocyclic Compounds; D013141:Spiro Compounds; C483569:spirotenuipesine A; C483570:spirotenuipesine B