Evaluation of DNA damage in a population of bats (Chiroptera) residing in an abandoned monazite mine.
Ionising radiation has the ability to induce DNA damage. While the effects of high doses of radiation of short duration have been well documented, the biological effects of long-term exposure to low doses are poorly understood. This study evaluated the clastogenic effects of low dose ionising radiation on a population of bats (Chiroptera) residing in an abandoned monazite mine. Bats were sampled from two chambers in the mine, where external radiation levels measured around 20 microSv/h (low dose) and 100 microSv/h (higher dose), respectively. A control group of bats was sampled from a cave with no detectable radiation above normal background levels. The micronucleus assay was used to evaluate residual radiation damage in binucleated lymphocytes and showed that the micronucleus frequency per 500 binucleated lymphocytes was increased in the lower radiation-exposed group (17.7) and the higher radiation-exposed group (27.1) compared to the control group (5.3). This study also showed that bats exposed to radiation presented with an increased number of micronuclei per one thousand reticulocytes (2.88 and 10.75 in the lower and high radiation-exposed groups respectively) when compared to the control group (1.7). The single-cell gel electrophoresis (comet) assay was used as a means of evaluating clastogenecity of exposure to radiation at the level of individual cells. Bats exposed to radiation demonstrated increased DNA damage as shown by the length of the comet tails and showed an increase in cumulative damage. The results of the micronucleus and the comet assays indicated not only a statistically significant difference between test and control groups (P<0.001), but also a dose-dependent increase in DNA damage (P<0.001). These assays may thus be useful in evaluating the potential clastogenecity of exposure to continuous low doses of ionising radiation.
Meehan
Kathleen A
KA
Faculty of Applied Sciences, Cape Technikon, P.O. Box 652, Cape Town 8000, South Africa. meehank@ctech.ac.za
Truter
Ernest J
EJ
Slabbert
Jacobus P
JP
Parker
M Iqbal
MI
D000818:Animals; D002685:Chiroptera / Q000235:genetics*; D020552:Comet Assay; D004249:DNA Damage; D008214:Lymphocytes / Q000528:radiation effects; D008674:Metals, Rare Earth / Q000633:toxicity*; D015162:Micronucleus Tests; D008906:Mining; D012156:Reticulocytes / Q000528:radiation effects
D008674:Metals, Rare Earth; C015370:monazite