Determination of glutathione peroxidase activity in human milk.
An analytical method for determining glutathione peroxidase (GPx) (EC 1.11.1.9) activity in whole blood has been adapted to human milk samples. The values obtained for precision (relative standard deviation: 8.4%), linearity and accuracy (recovery: 90.4%) indicate the adequacy of the method for the mentioned purpose. The method was applied to 11 human milk samples obtained in the range from 6 to 135 days post partum. The resulting GPx activities (83.3 +/- 23.6 U/L) did not correlate to the selenium content of the samples, though a reciprocal correlation was found between the duration of lactation and milk GPx activity.
Torres
Angeles
A
Nutrition and Food Chemistry Faculty of Pharmacy, Valencia University, Burjassot, Valencia, Spain.
Farré
Rosaura
R
Lagarda
Maria Jesús
MJ
Monleón
Javier
J
D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D007774:Lactation / Q000502:physiology; D008895:Milk, Human / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
D005979:Glutathione Peroxidase