Detailed criteria for the assessment of clinical symptoms in a new murine model of severe allergic conjunctivitis.
OBJECTIVE
The aims of this research were to: (1) generate a rapid protocol for the sensitization of rodents to a defined allergen without footpad injections yet leading to both acute- and late-phase hypersensitivity reactions in the ocular surface; and (2) define detailed criteria for the assessment of clinical symptoms in the acute-phase response.


METHODS
With the approved methods for the use of experimental animals in research and existing sensitization protocols as a starting point, we developed and tested a new protocol with respect to its ability to generate an acute- and late-phase response on ocular challenge. Clinical symptoms were assessed by a trained ophthalmologist under masked conditions, and late-phase responses determined by histologic analysis of conjunctival tissue sections.


RESULTS
A new protocol for the rapid sensitization of mice, avoiding footpad injections, yet yielding both acute- and late-phase allergic responses, was developed. Detailed criteria for the assessment of disease severity were established and tested.


CONCLUSIONS
This protocol establishes a murine model of allergic conjunctivitis that will be useful for both the study of the molecular and cellular basis of allergic reactions in the ocular surface and the testing of new therapies for this disease.
Nakamura
Takao
T
Department of Immunology, Institutes of Ophthalmology and Child Health, University College London, University of London, London, UK.
Toda
Masako
M
Ohbayashi
Masaharu
M
Ono
Santa Jeremy
SJ
D031164:Ambrosia / Q000276:immunology; D000818:Animals; D003233:Conjunctivitis, Allergic / Q000503:physiopathology*; D004195:Disease Models, Animal; D006968:Hypersensitivity, Delayed / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007114:Immunization / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains; D011058:Pollen / Q000276:immunology; D012720:Severity of Illness Index