title: Expression of endoglin in choroidal neovascularization.
abstract: Endoglin (CD105) is a membrane protein involved in the TGF-beta receptor signalling pathway with predominant expression by proliferating endothelial cells. The aim of this study is to analyze the expression of Endoglin in choroidal neovascularization membranes (CNVM) and to compare it to the overall proliferative status of CNVM. Thirty surgically excised CNVM, secondary to age-related macular degeneration, were investigated using light microscopic immunohistochemistry and confocal immunofluorescence microscopy using verified antibodies directed against the endothelial cell markers Endoglin, von Willebrand factor (vWF) and CD34 and the proliferation marker Ki-67. Donor eyes were used as controls. A selective expression of CD34 and vWF as well as Endoglin was found in endothelial cells. Endoglin expression was elevated in vascular endothelial cells contained within CNVM, but a moderate Endoglin expression could also be visualized in quiescent CD34 and vWF positive ocular vasculature. Ki-67 positive cells were detected in CNVM, but these were rarely endothelial cells. Endoglin expression in endothelial cells of CNVM is increased, but rarely associated with a concomitant expression of the proliferation marker Ki-67. The elevated expression of Endoglin in surgically excised CNVM suggests a persisting post-mitotic activation in an advanced stage of this neovascular tissue.
authors.affiliation: Department of Vitreoretinal Surgery, Center of Ophthalmology, Eberhard-Karls University Tuebingen, Schleichstrasse 12-15, FRG 72070 Tuebingen, Germany. salvatore.grisanti@uni-tuebingen.de
authors.forename: Salvatore
authors.initials: S
authors.lastname: Grisanti
authors.forename: Serap
authors.initials: S
authors.lastname: Canbek
authors.forename: Edwin
authors.initials: E
authors.lastname: Kaiserling
authors.forename: Anne
authors.initials: A
authors.lastname: Adam
authors.forename: Bart
authors.initials: B
authors.lastname: Lafaut
authors.forename: Faik
authors.initials: F
authors.lastname: Gelisken
authors.forename: Peter
authors.initials: P
authors.lastname: Szurman
authors.forename: Sigrid
authors.initials: S
authors.lastname: Henke-Fahle
authors.forename: Jolanta
authors.initials: J
authors.lastname: Oficjalska-Mlynczak
authors.forename: Karl Ulrich
authors.initials: KU
authors.lastname: Bartz-Schmidt
mesh_terms: D015703:Antigens, CD; D018952:Antigens, CD34 / Q000378:metabolism; D002455:Cell Division; D020256:Choroidal Neovascularization / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D000071063:Endoglin; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism; D008268:Macular Degeneration / Q000150:complications; D018613:Microscopy, Confocal; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D005136:Eye Proteins; D019394:Ki-67 Antigen; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D014841:von Willebrand Factor