[DNA microarray for the detection of Yersinia pesits].
OBJECTIVE
To develop a DNA microarray technique for fast diagnosis of plague.


METHODS
Restriction display polymerase chain reaction (RD-PCR) and hybridization with fluorescently labeled cy5 were employed for detecting the sample DNA of Yersinia pestis, Yersinia pseudotuberculosis and Yersinia enterocolitica.


RESULTS
The prepared microarray is capable of distinguishing Yersinia pestis from Yersinia pseudotuberculosis and Yersinia enterocolitica in the same genus.


CONCLUSIONS
The constructed DNA microarray is an effective diagnostic tool for the detection of Yersinia pestis.
Huang
Hai
H
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Dong
Xing-qi
XQ
Zhang
Bao
B
Wu
Qinghua
Q
Zheng
Wen-ling
WL
D005453:Fluorescence; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015010:Yersinia pestis / Q000302:isolation & purification*