title: Cellular and humoral responses to collagen-polyvinylpyrrolidone administered during short and long periods in humans.
abstract: Collagen, particularly type I, and its related derivatives have been extensively employed in many areas of pharmacology. The present study was performed to determine the safety of collagen-polyvinylpyrrolidone (collagen-PVP) by in vitro and in vivo studies. Sera and peripheral blood cells from healthy donors without treatment and patients treated with collagen-PVP were evaluated. We observed that the biodrug does not stimulate lymphoproliferation or DNA damage in vitro, nor does it induce human anti-porcine type I collagen or anti-collagen-PVP antibodies in vivo. Furthermore, no hepatic or renal metabolic dysfunctions were observed when collagen-PVP was administered by intradermal or intramuscular routes in short- or long-term treatments. In conclusion, the present work shows that no cellular damage or immunological adverse effects (cellular and humoral) occurred during collagen-PVP treatment, even after more than 400 weeks of consecutive administrations.
authors.affiliation: Department of Cellular Biology, Instituto de Investigaciones Biomédicas, UNAM, Ciudad Universitaria, Mexico City, Mexico.
authors.forename: Janette
authors.initials: J
authors.lastname: Furuzawa-Carballeda
authors.forename: Emilio
authors.initials: E
authors.lastname: Rojas
authors.forename: Mahara
authors.initials: M
authors.lastname: Valverde
authors.forename: Irma
authors.initials: I
authors.lastname: Castillo
authors.forename: Lino
authors.initials: L
authors.lastname: Diaz de León
authors.forename: Edgar
authors.initials: E
authors.lastname: Krötzsch
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage* / Q000009:adverse effects / Q000276:immunology*; D024042:Collagen Type I / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004334:Drug Administration Schedule; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D011205:Povidone / Q000008:administration & dosage* / Q000009:adverse effects; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D012882:Skin Tests; D018709:Statistics, Nonparametric; D013552:Swine
chemical_list: D024042:Collagen Type I; C120488:collagen-PVP; D003094:Collagen; D011205:Povidone