Effect of fermentation broth and biosurfactants on mass transfer during liquid-liquid extraction.
Mass transfer rates in liquid-liquid extraction processes can be seriously affected by the presence of surface-active contaminants. This is especially true of applications of a biotechnological origin, where the microorganism used in the process may produce the surface-active contaminants. An investigation into the effects of soluble and insoluble fermentation broth components on mass transfer using chloramphenicol extraction into octanol as the model system was conducted. Soluble components produced during fermentation were found to adsorb to the interface, where they reduced the overall mass transfer coefficient by up to 70%. After fractionation it was found that components in the weight range from 10-30 kDa had the greatest effect on mass transfer. Protein and phospholipid compounds of similar size were found to reduce the overall mass transfer coefficient to a similar extent to the broth components at concentrations around 0.001mg/l. The biomass produced during the fermentation also reduced mass transfer substantially, and it is likely that this was due to physical blockage of the interface.
Pursell
Mark R
MR
Department of Chemical Engineering and Chemical Technology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London, United Kingdom SW7 2BY.
Mendes-Tatsis
M Alcina
MA
Stuckey
David C
DC
D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002701:Chloramphenicol / Q000096:biosynthesis / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D008567:Membranes, Artificial; D009038:Motion; D010865:Pilot Projects; D012212:Rheology / Q000379:methods; D012996:Solutions; D013302:Streptomyces / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism; D014462:Ultrafiltration / Q000379:methods*
D003470:Culture Media; D008567:Membranes, Artificial; D012996:Solutions; D013501:Surface-Active Agents; D002701:Chloramphenicol