Transcriptional silencing of the RUNX3 gene by CpG hypermethylation is associated with lung cancer.
RUNX family transcription factors are integral components of TGF-beta signaling pathways and have been implicated in cell cycle regulation, differentiation, apoptosis, and malignant transformation. It was noted previously that allele loss and loss of expression of RUNX3 are causally involved in gastric carcinogenesis. Our results demonstrate that RUNX3 is inactivated by aberrant DNA methylation in approximately 19% of lung cancer cell lines and 24% of primary lung cancer specimens. RUNX3 methylation is tumor-specific, since it is not observed in surrounding normal lung tissues. Our results suggest that loss of RUNX3 expression by DNA hypermethylation is frequently associated with the evolution of lung cancer.
Li
Qing-Lin
QL
Department of Biochemistry, School of Medicine and Institute for Tumor Research, Chungbuk National University, Cheongju, Republic of Korea.
Kim
Hye-Ryeon
HR
Kim
Wun-Jae
WJ
Choi
Joong-Kook
JK
Lee
Yong Hee
YH
Kim
Hwan-Mook
HM
Li
Long Shan
LS
Kim
Hoguen
H
Chang
Joon
J
Ito
Yoshiaki
Y
Youl Lee
Kwang
K
Bae
Suk-Chul
SC
D001483:Base Sequence; D045744:Cell Line, Tumor; D050677:Core Binding Factor Alpha 3 Subunit; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D037941:Silencer Elements, Transcriptional / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D050677:Core Binding Factor Alpha 3 Subunit; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C493729:Runx3 protein, human; D014157:Transcription Factors