title: Flow cytometry and T-cell response monitoring after smallpox vaccination.
abstract: Orthopoxvirus zoonosis or smallpox as result of bioterrorism or biological warfare represents a risk for epidemic spread. By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox. When the high similarity between the orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures.
authors.affiliation: National Institute for Infectious Diseases "Lazzaro Spallanzani," Rome, Italy. poccia@inmi.it
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Poccia
authors.forename: Cristiana
authors.initials: C
authors.lastname: Gioia
authors.forename: Carla
authors.initials: C
authors.lastname: Montesano
authors.forename: Federico
authors.initials: F
authors.lastname: Martini
authors.forename: Douglas
authors.initials: D
authors.lastname: Horejsh
authors.forename: Concetta
authors.initials: C
authors.lastname: Castilletti
authors.forename: Leopoldo paolo
authors.initials: Lp
authors.lastname: Pucillo
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Capobianchi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ippolito
mesh_terms: D000956:Antigens, Viral / Q000302:isolation & purification*; D005434:Flow Cytometry; D006801:Humans; D012899:Smallpox / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D012900:Smallpox Vaccine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D000956:Antigens, Viral; D012900:Smallpox Vaccine