Transformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans.
Transformation systems for Aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of Aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrG) of Aspergillus nidulans. An A. aculeatus mutant which can be transformed effectively by the A. nidulans pyrG gene was isolated as a transformation host. This is the first report of transformation of A. aculeatus.
Kanamasa
Shin
S
Graduate School of Agriculture and Biological Sciences, and Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Japan.
Yamaoka
Kana
K
Kawaguchi
Takashi
T
Sumitani
Jun-ichi
J
Arai
Motoo
M
D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D001236:Aspergillus oryzae / Q000235:genetics*; D015139:Blotting, Southern; D005822:Genetic Vectors; D009154:Mutation; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D011523:Protoplasts / Q000166:cytology; D011745:Pyrithiamine / Q000737:chemistry; D014170:Transformation, Genetic
D011745:Pyrithiamine; D009964:Orotidine-5'-Phosphate Decarboxylase