A set of commercially available fluorescent in-situ hybridization probes efficiently detects cytogenetic abnormalities in patients with chronic lymphocytic leukemia.
OBJECTIVE
To investigate a simplified panel of fluorescent in-situ hybridization (FISH) probes for evaluation of patients with chronic lymphocytic leukemia (CLL) and to correlate results from this technique with known prognostic factors.


METHODS
We retrospectively reviewed the FISH and conventional cytogenetic results, and clinical and laboratory data of 44 patients with CLL.


RESULTS
FISH was more sensitive than conventional cytogenetics in detecting genomic aberrations (75% vs. 16%, P < 0.0001). Trisomy 12 was significantly correlated with the cell surface marker of CD38 expression (P = 0.0017).


CONCLUSIONS
This FISH panel reliably detects prognostically important genomic abnormalities in CLL and is suitable for widespread use.
Goorha
Salil
S
Department of Internal Medicine, Division of Clinical Oncology, University of Utah School of Medicine, Salt Lake City, Utah, USA.
Glenn
Martha J
MJ
Drozd-Borysiuk
Elizabeth
E
Chen
Zhong
Z
D036541:ADP-ribosyl Cyclase / Q000096:biosynthesis; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D002881:Chromosomes, Human, Pair 12; D015342:DNA Probes; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D018570:Risk Assessment; D014314:Trisomy
D015703:Antigens, CD; D015342:DNA Probes; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1