Investigation of killer cell immunoglobulinlike receptor gene diversity: I. KIR2DL4.
Polymerase chain reaction-sequence-specific oligonucleotide probes typing procedures identifying alleles of the killer immunoglobulin-like gene (KIR2DL4) have been established. The methods, designed around the specific amplification of the D0 and D2 domains of this gene, produce discrimination of KIR2DL4 alleles. The methods have been applied to a healthy Northern Irish control group, establishing frequencies for this Caucasian population. Additionally, the KIR2DL4 allele status of cell line DNA and CEPH families, from the 13th International Histocompatibility Workshop and local families, have also been investigated.
Williams
Fionnuala
F
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland. fionnuala.williams@bll.n-i.nhs.uk
Meenagh
Ashley
A
Sleator
Carole
C
Middleton
Derek
D
D000483:Alleles; D002460:Cell Line; D003462:Cuba; D005006:Ethnicity / Q000235:genetics; D005091:Exons / Q000235:genetics; D005602:France; D005787:Gene Frequency; D006723:Hong Kong; D006801:Humans; D009660:Northern Ireland; D009850:Oman; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR; D054345:Receptors, KIR2DL4; D013019:South Africa; D044465:White People / Q000235:genetics
C515079:KIR2DL4 protein, human; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054345:Receptors, KIR2DL4