Identification of the primary electron donor in PS II of the Chl d-dominated cyanobacterium Acaryochloris marina.
The primary electron donor of photosystem (PS) II in the chlorophyll (Chl) d-dominated cyanobacterium Acaryochloris marina was confirmed by delayed fluorescence (DF) and further proved by pigment contents of cells grown under several light intensities. The DF was found only in the Chl a region, identical to Synechocystis sp. PCC 6803, and disappeared following heat treatment. Pigment analyses indicated that at least two Chl a molecules were present per each two pheophytin a molecules, and these Chl a molecules are assigned to P(D1) and P(D2). These findings clearly indicate that Chl a is required for water oxidation in PS II.
Mimuro
Mamoru
M
Department of Technology and Ecology, Hall of Global Environmental Research, Kyoto University, 606-8501, Kyoto, Japan. mamo_mi@bio.kyoto-u.ac.jp
Akimoto
Seiji
S
Gotoh
Takanori
T
Yokono
Makio
M
Akiyama
Machiko
M
Tsuchiya
Tohru
T
Miyashita
Hideaki
H
Kobayashi
Masami
M
Yamazaki
Iwao
I
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis / Q000737:chemistry*; D000077194:Chlorophyll A; D000458:Cyanobacteria / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D004579:Electron Transport; D005453:Fluorescence; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; C107509:chlorophyll d; D000077194:Chlorophyll A