[Effect of Sinorhizobium fredii YC4 symbiotic plasmid amplification on nod factors and symbiotic N-fixation].
Sinorhizobium fredii YC4 can form nitrogen-fixing nodules on soybean (Glycine max) and wild soybean (G. soja). It can produce unique lipochitooligosaccharide nod factors (LCOs), in comparison with the other four strains of S. fredii. The constitution of LCOs produced by YC4 contained more hydrophobic substitutions detected by Thin-layer chromatography (TLC) of 14C-labeled nod factors. A spontaneous mutant termed YSC3 amplified in the symbiotic plasmid was isolated from YC4, which can produce more amount of LCOs than its parental strain at 28 degrees C, and showed a difference in the construction of LCOs. Nodulation test indicated that YSC3 only formed ineffective nodules on soybean G. soja.
Miao
Lihong
L
Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. miaolih2001@163.com
Zhou
Junchu
J
D008070:Lipopolysaccharides / Q000096:biosynthesis*; D009586:Nitrogen Fixation; D010957:Plasmids; D020579:Sinorhizobium / Q000235:genetics*; D013025:Glycine max / Q000382:microbiology*; D013559:Symbiosis; D013696:Temperature
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides