Metabolic alterations of toxic and nonessential elements by the treatment of Sempervivum tectorum extract in a hyperlipidemic rat model.
A hyperlipidemic rat model was used to examine the therapeutic effect of Sempervivum tectorum plant extract on the metabolic alterations of Al, As, B, Ba, Cd, Hg, Ni, Pb, and Ti in the liver and bile. Hyperlipidemia was produced by lipogenic diet and alcohol and verified by morphological investigation of the liver with the aid of light and an electron microscope. Element concentration in the liver and bile were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The concentration values in the liver higher than the detection limit (Al, Ba, Ni, Ti) were unambiguous. Significant differences were found for the four groups at p < 0.05 level (ANOVA). A significant difference was observed between Al and B concentration in the bile fluids of the 4 groups (p < 0.05). The excretion of Al and Ti into the bile fluid increased significantly (p < 0.05). Following the administration of S. tectorum extract to rats with hyperlipidemia, the excretion of Al, B and Ba increased, whereas the excretion of Ti decreased significantly (p < 0.05). The favorable action of the extract (protecting the liver in hyperlipidemic rats) was verified by morphological studies, and its detoxicating property was shown by the elimination of Al, Ba, Ni, and Ti from the liver.
Szentmihályi
Klára
K
Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary. szklari@chemres.hu
Fehér
Erzsébet
E
Vinkler
Péter
P
Kéry
Agnes
A
Blázovics
Anna
A
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism; D029627:Crassulaceae / Q000737:chemistry*; D004032:Diet; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D022781:Hepatocytes / Q000187:drug effects / Q000648:ultrastructure; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008658:Inactivation, Metabolic; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008670:Metals / Q000032:analysis / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D028761:Withholding Treatment
D000960:Hypolipidemic Agents; D008670:Metals; D010936:Plant Extracts