Molecular characterization of Enterocytozoon bieneusi in cattle indicates that only some isolates have zoonotic potential.
In this study, 338 fecal samples were analyzed for Enterocytozoon bieneusi from cattle farms in Florida, Maryland, Michigan, New York, North Carolina, Pennsylvania, Virginia, and Portugal. The internal transcribed spacer region (392 bp) of the rRNA gene of E. bieneusi was amplified using a nested PCR protocol. Thirty-two E. bieneusi-PCR positive samples were sequenced. A high degree of genetic polymorphism, represented by five distinct genotypes (BEB1-BEB5), was found among the E. bieneusi isolates from cattle. Most of the isolates formed a distinct cluster consisting of only the four cattle genotypes, but six isolates of a genotype clustered together with E. bieneusi genotypes from humans and other domestic animals. Therefore, only some E. bieneusi isolates from cattle may be of public health importance.
Sulaiman
Irshad M
IM
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
Fayer
Ron
R
Yang
Chunfu
C
Santin
Monica
M
Matos
Olga
O
Xiao
Lihua
L
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology* / Q000635:transmission; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D020459:Genes, rRNA; D005838:Genotype; D016881:Microsporidiosis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011174:Portugal; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014481:United States; D015047:Zoonoses
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer