[Effect of tumor necrosis factor-alpha and interleukin-1beta on bovine pulmonary arterial endothelial cell apoptosis].
OBJECTIVE
To investigate the effect of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) on apoptosis of bovine pulmonary arterial endothelial cells (BPEC).


METHODS
BPECs were incubated with TNF-alpha, IL-1beta or the combination of TNF-alpha and IL-1beta. BEPCs were also pretreated with TNF-alpha monoclonal antibody or Ac-DEVD-CHO (caspase-3 inhibitor), and followed by incubation with TNF-alpha. The apoptotic rate was measured by flow cytometry (Annexin V-FITC and PI staining).


RESULTS
(1) TNF-alpha induced apoptosis in a dose-dependent manner after a 24 h incubation. With the increase of the TNF-alpha (500, 1,000, 2,000 U/ml respectively), the BPEC apoptotic rate [(8.21 +/- 0.70)%, (9.63 +/- 0.71)%, (17.43 +/- 1.99)%, respectively] was significantly higher than that of the control group [(3.09 +/- 0.08)%, P < 0.05]. (2) TNF-alpha induced apoptosis was time dependent. After incubated with TNF-alpha 2,000 U/ml, the BPEC apoptotic rate [(6.72 +/- 0.38)%, (7.72 +/- 1.66)%, (12.95 +/- 0.32)%, (17.70 +/- 1.79)%, P < 0.05] increased significantly with time [6, 12, 24, 36 h, respectively] of TNF-alpha incubation. (3) The BPEC apoptotic rate of anti-TNF-alpha monoclonal antibody group [(7.78 +/- 0.21)%] or the Ac-DEVD-CHO group [(7.32 +/- 0.11)%] was significantly higher than the TNF-alpha group alone [(10.59 +/- 0.49)%, P < 0.01], but the combination of TNF-alpha and IL-1beta group had no such significant effect (P > 0.05).


CONCLUSIONS
It is TNF-alpha but not IL-1beta that induces pulmonary arterial endothelial cell apoptosis in acute lung injury (ALI). There is no synergistic effect between IL-1beta and TNF-alpha in the induction of BPEC apoptosis.
Nong
Ling-bo
LB
Guangzhou Institute of Respiratory Diseases, First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China.
Xiao
Zheng-lun
ZL
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D007375:Interleukin-1 / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects*; D012128:Respiratory Distress Syndrome / Q000209:etiology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha