Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis.
Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin-a, microcystin-LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1-4 microg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.
Vasas
Gábor
G
Department of Botany, Faculty of Science, University of Debrecen, Debrecen, Hungary.
Gáspár
Attila
A
Páger
Csilla
C
Surányi
Gyula
G
Máthé
Csaba
C
Hamvas
Márta M
MM
Borbely
George
G
D000470:Alkaloids; D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification*; D000087522:Cyanobacteria Toxins; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D008387:Marine Toxins / Q000032:analysis / Q000302:isolation & purification*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis / Q000302:isolation & purification; D014326:Tropanes; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D014873:Water Pollutants / Q000032:analysis
D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D014326:Tropanes; D014873:Water Pollutants; C089595:cylindrospermopsin; D014498:Uracil; C509783:anatoxin a; C057862:cyanoginosin LR