Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli.
Human interferon-gamma (hIFN-gamma) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l(-1) under both constant (0.12 h(-1)) and variable (0.12-0.52 h(-1)) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-gamma were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-gamma were 0.35 +/- 0.02 g rhIFN-gamma g(-1) dry cell wt and 0.9 +/- 0.05 g rhIFN-gamma l(-1) h(-1) in the variable specific growth rate fed-batch process, respectively.
Khalilzadeh
R
R
Biotechnology Group, Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modarres University, Tehran, P.O. Box 14155-4838, Iran.
Shojaosadati
S A
SA
Bahrami
A
A
Maghsoudi
N
N
D019149:Bioreactors / Q000382:microbiology*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008954:Models, Biological; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; D007371:Interferon-gamma; D005947:Glucose